LOCUS       DAVBNO010000001        75386 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVBNO010000001 DAVBNO010000000
VERSION     DAVBNO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 75386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75386
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.001"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(141..1010)
                     /locus_tag="VQ846_000001"
     CDS             complement(141..1010)
                     /locus_tag="VQ846_000001"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET3074673.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            complement(1012..2298)
                     /locus_tag="VQ846_000002"
     CDS             complement(1012..2298)
                     /locus_tag="VQ846_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET3074674.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(2449..3186)
                     /gene="mecA"
                     /locus_tag="VQ846_000003"
     CDS             complement(2449..3186)
                     /gene="mecA"
                     /locus_tag="VQ846_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET3074675.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(3386..4420)
                     /locus_tag="VQ846_000004"
     CDS             complement(3386..4420)
                     /locus_tag="VQ846_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET3074676.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(4449..5882)
                     /locus_tag="VQ846_000005"
     CDS             complement(4449..5882)
                     /locus_tag="VQ846_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET3074677.1"
                     /translation="MIDGKRLLFSLTIVSYTLTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            complement(5898..6884)
                     /locus_tag="VQ846_000006"
     CDS             complement(5898..6884)
                     /locus_tag="VQ846_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET3074678.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAKKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGYAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(6886..7989)
                     /locus_tag="VQ846_000007"
     CDS             complement(6886..7989)
                     /locus_tag="VQ846_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET3074679.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFNVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(7979..8824)
                     /locus_tag="VQ846_000008"
     CDS             complement(7979..8824)
                     /locus_tag="VQ846_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET3074680.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKKVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(9103..10377)
                     /gene="psr"
                     /locus_tag="VQ846_000009"
     CDS             complement(9103..10377)
                     /gene="psr"
                     /locus_tag="VQ846_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET3074681.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(10374..11222)
                     /gene="pheA"
                     /locus_tag="VQ846_000010"
     CDS             complement(10374..11222)
                     /gene="pheA"
                     /locus_tag="VQ846_000010"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET3074682.1"
                     /translation="MKIAYLGPKGSFSHHVVQIAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(11219..11695)
                     /locus_tag="VQ846_000011"
     CDS             complement(11219..11695)
                     /locus_tag="VQ846_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET3074683.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(11688..12971)
                     /gene="aroA"
                     /locus_tag="VQ846_000012"
     CDS             complement(11688..12971)
                     /gene="aroA"
                     /locus_tag="VQ846_000012"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET3074684.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMLGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSSTLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(13063..13401)
                     /locus_tag="VQ846_000013"
     CDS             complement(13063..13401)
                     /locus_tag="VQ846_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET3074685.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAHTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(13412..14515)
                     /locus_tag="VQ846_000014"
     CDS             complement(13412..14515)
                     /locus_tag="VQ846_000014"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET3074686.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(14525..15691)
                     /gene="aroC"
                     /locus_tag="VQ846_000015"
     CDS             complement(14525..15691)
                     /gene="aroC"
                     /locus_tag="VQ846_000015"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET3074687.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(15701..16768)
                     /locus_tag="VQ846_000016"
     CDS             complement(15701..16768)
                     /locus_tag="VQ846_000016"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET3074688.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            complement(16787..17641)
                     /locus_tag="VQ846_000017"
     CDS             complement(16787..17641)
                     /locus_tag="VQ846_000017"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET3074689.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATDTNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            complement(17631..18308)
                     /locus_tag="VQ846_000018"
     CDS             complement(17631..18308)
                     /locus_tag="VQ846_000018"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET3074690.1"
                     /translation="MKLIVSVMPRSLEEAQALDDTRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(18305..19468)
                     /locus_tag="VQ846_000019"
     CDS             complement(18305..19468)
                     /locus_tag="VQ846_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET3074691.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHHFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(20155..21789)
                     /locus_tag="VQ846_000020"
     CDS             complement(20155..21789)
                     /locus_tag="VQ846_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074692.1"
                     /translation="MRLKVIKKLVDINILYSSQDANLANPRKKQAKNTGKKVNVSARV
                     LSSYIFSSLLMLIFFSTIAIHFPFEEMPVYFSFMVAILLVIAFSTSLTAFYNVFYESK
                     DLASYSPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVTLVTVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSIEAILGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLPSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPILTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(21770..22519)
                     /locus_tag="VQ846_000021"
     CDS             complement(21770..22519)
                     /locus_tag="VQ846_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3074693.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRKDNPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            22698..24152
                     /locus_tag="VQ846_000022"
     CDS             22698..24152
                     /locus_tag="VQ846_000022"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET3074694.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAEDAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHMEAFQVIEVDPVDRTVELGEPFTINGWTSFTFYGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKSVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQQDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(24241..26859)
                     /gene="alaS"
                     /locus_tag="VQ846_000023"
     CDS             complement(24241..26859)
                     /gene="alaS"
                     /locus_tag="VQ846_000023"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET3074695.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLVIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDVVAGKL"
     gene            complement(26881..27366)
                     /locus_tag="VQ846_000024"
     CDS             complement(26881..27366)
                     /locus_tag="VQ846_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET3074696.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(27641..27880)
                     /locus_tag="VQ846_000025"
     CDS             complement(27641..27880)
                     /locus_tag="VQ846_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074697.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(28141..29211)
                     /locus_tag="VQ846_000026"
     CDS             complement(28141..29211)
                     /locus_tag="VQ846_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET3074698.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIKGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(29208..29981)
                     /locus_tag="VQ846_000027"
     CDS             complement(29208..29981)
                     /locus_tag="VQ846_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3074699.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(29978..30784)
                     /locus_tag="VQ846_000028"
     CDS             complement(29978..30784)
                     /locus_tag="VQ846_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3074700.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITFGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(30765..31922)
                     /locus_tag="VQ846_000029"
     CDS             complement(30765..31922)
                     /locus_tag="VQ846_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3074701.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVVF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(32082..32987)
                     /gene="murB"
                     /locus_tag="VQ846_000030"
     CDS             complement(32082..32987)
                     /gene="murB"
                     /locus_tag="VQ846_000030"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET3074702.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(33099..33851)
                     /locus_tag="VQ846_000031"
     CDS             complement(33099..33851)
                     /locus_tag="VQ846_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HET3074703.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFTKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(33861..34592)
                     /gene="budA"
                     /locus_tag="VQ846_000032"
     CDS             complement(33861..34592)
                     /gene="budA"
                     /locus_tag="VQ846_000032"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET3074704.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKESGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADIATHQPEYSRDNV
                     AGTIVGFWTPQIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            34734..36005
                     /locus_tag="VQ846_000033"
     CDS             34734..36005
                     /locus_tag="VQ846_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET3074705.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFDQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRAILDLAAKYSVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNTIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(36025..36851)
                     /locus_tag="VQ846_000034"
                     /pseudo
     CDS             complement(36025..36851)
                     /locus_tag="VQ846_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            complement(37008..37661)
                     /gene="phoU"
                     /locus_tag="VQ846_000035"
     CDS             complement(37008..37661)
                     /gene="phoU"
                     /locus_tag="VQ846_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET3074706.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(37673..38431)
                     /gene="pstB"
                     /locus_tag="VQ846_000036"
     CDS             complement(37673..38431)
                     /gene="pstB"
                     /locus_tag="VQ846_000036"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET3074707.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(38444..39247)
                     /gene="pstB"
                     /locus_tag="VQ846_000037"
     CDS             complement(38444..39247)
                     /gene="pstB"
                     /locus_tag="VQ846_000037"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET3074708.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(39258..40142)
                     /gene="pstA"
                     /locus_tag="VQ846_000038"
     CDS             complement(39258..40142)
                     /gene="pstA"
                     /locus_tag="VQ846_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET3074709.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(40132..41049)
                     /gene="pstC"
                     /locus_tag="VQ846_000039"
     CDS             complement(40132..41049)
                     /gene="pstC"
                     /locus_tag="VQ846_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET3074710.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(41049..41927)
                     /gene="pstS"
                     /locus_tag="VQ846_000040"
     CDS             complement(41049..41927)
                     /gene="pstS"
                     /locus_tag="VQ846_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET3074711.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(42501..43805)
                     /locus_tag="VQ846_000041"
     CDS             complement(42501..43805)
                     /locus_tag="VQ846_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET3074712.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(43806..44579)
                     /locus_tag="VQ846_000042"
     CDS             complement(43806..44579)
                     /locus_tag="VQ846_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET3074713.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLASFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(44569..44847)
                     /locus_tag="VQ846_000043"
     CDS             complement(44569..44847)
                     /locus_tag="VQ846_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET3074714.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(44847..45248)
                     /locus_tag="VQ846_000044"
     CDS             complement(44847..45248)
                     /locus_tag="VQ846_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET3074715.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQQ
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(45458..46075)
                     /locus_tag="VQ846_000045"
     CDS             complement(45458..46075)
                     /locus_tag="VQ846_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET3074716.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLPIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(46065..46838)
                     /locus_tag="VQ846_000046"
     CDS             complement(46065..46838)
                     /locus_tag="VQ846_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET3074717.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(46849..47586)
                     /locus_tag="VQ846_000047"
     CDS             complement(46849..47586)
                     /locus_tag="VQ846_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET3074718.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWKEIKKA"
     gene            complement(47591..48721)
                     /locus_tag="VQ846_000048"
     CDS             complement(47591..48721)
                     /locus_tag="VQ846_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET3074719.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLNLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(48805..49194)
                     /locus_tag="VQ846_000049"
     CDS             complement(48805..49194)
                     /locus_tag="VQ846_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET3074720.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(49210..49593)
                     /locus_tag="VQ846_000050"
     CDS             complement(49210..49593)
                     /locus_tag="VQ846_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET3074721.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(49594..50382)
                     /locus_tag="VQ846_000051"
     CDS             complement(49594..50382)
                     /locus_tag="VQ846_000051"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET3074722.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(50375..51310)
                     /locus_tag="VQ846_000052"
     CDS             complement(50375..51310)
                     /locus_tag="VQ846_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET3074723.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINIADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(51440..51616)
                     /locus_tag="VQ846_000053"
     CDS             complement(51440..51616)
                     /locus_tag="VQ846_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET3074724.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(51756..52463)
                     /locus_tag="VQ846_000054"
     CDS             complement(51756..52463)
                     /locus_tag="VQ846_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET3074725.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            52614..53642
                     /gene="queA"
                     /locus_tag="VQ846_000055"
     CDS             52614..53642
                     /gene="queA"
                     /locus_tag="VQ846_000055"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET3074726.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKMETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(54303..54692)
                     /locus_tag="VQ846_000056"
     CDS             complement(54303..54692)
                     /locus_tag="VQ846_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET3074727.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(54746..55437)
                     /locus_tag="VQ846_000057"
                     /pseudo
     CDS             complement(54746..55437)
                     /locus_tag="VQ846_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
     gene            complement(56152..56703)
                     /locus_tag="VQ846_000058"
     CDS             complement(56152..56703)
                     /locus_tag="VQ846_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3074728.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSICNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(57053..57877)
                     /gene="nadE"
                     /locus_tag="VQ846_000059"
     CDS             complement(57053..57877)
                     /gene="nadE"
                     /locus_tag="VQ846_000059"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET3074729.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(57874..59334)
                     /locus_tag="VQ846_000060"
     CDS             complement(57874..59334)
                     /locus_tag="VQ846_000060"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET3074730.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(59450..59938)
                     /locus_tag="VQ846_000061"
     CDS             complement(59450..59938)
                     /locus_tag="VQ846_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074731.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(59928..60137)
                     /locus_tag="VQ846_000062"
     CDS             complement(59928..60137)
                     /locus_tag="VQ846_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3074732.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(60523..61003)
                     /locus_tag="VQ846_000063"
                     /pseudo
     CDS             complement(60523..61003)
                     /locus_tag="VQ846_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(61163..61897)
                     /locus_tag="VQ846_000064"
     CDS             complement(61163..61897)
                     /locus_tag="VQ846_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3074733.1"
                     /translation="MRIKEKTNNINGGIKNVSKHYGHSIILKDINFALNKGEIVGLAG
                     RNGVGKSTLMKILVQNNQPTSGNIISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGV
                     DYNQERFRCLIQELDLTQSINKKVKTYSLGTKQKLALLLTLVTEPDILILDEPTNGLD
                     IESSQIVLAVLKKLALHENVGILISSHKLEDIEEICERVLFLENGLLTFQKVGKDSHN
                     FLFEIAFSSATDRDIFITKQEFWDIV"
     gene            complement(62232..62702)
                     /locus_tag="VQ846_000065"
     CDS             complement(62232..62702)
                     /locus_tag="VQ846_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HET3074734.1"
                     /translation="MDAYLESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQ
                     LFGARRKIPEYKFVAEVISYDDATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDL
                     HDAKGNKIDRAPNPMELLTIKVPQPVQAGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            complement(62723..62962)
                     /locus_tag="VQ846_000066"
     CDS             complement(62723..62962)
                     /locus_tag="VQ846_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET3074735.1"
                     /translation="MRDANRGGCSQSCRWKYDLYDMPFGKERKSLQGEIPEEFSMSAV
                     DMSMIDHIPDMIENGVDNLKIEGRMEWSLFTTYQQ"
     gene            complement(63211..63522)
                     /locus_tag="VQ846_000067"
     CDS             complement(63211..63522)
                     /locus_tag="VQ846_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074736.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(63537..64823)
                     /locus_tag="VQ846_000068"
     CDS             complement(63537..64823)
                     /locus_tag="VQ846_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET3074737.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDATQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQAGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            65446..65628
                     /locus_tag="VQ846_000069"
     CDS             65446..65628
                     /locus_tag="VQ846_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET3074738.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            65638..65988
                     /locus_tag="VQ846_000070"
     CDS             65638..65988
                     /locus_tag="VQ846_000070"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET3074739.1"
                     /translation="MPNSESTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYGDSAWEDLRAVTGID
                     LLSILTDIANKKQG"
     gene            65996..67186
                     /locus_tag="VQ846_000071"
     CDS             65996..67186
                     /locus_tag="VQ846_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET3074740.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     DLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(67701..68693)
                     /locus_tag="VQ846_000072"
     CDS             complement(67701..68693)
                     /locus_tag="VQ846_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3074741.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQSNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            68853..70607
                     /locus_tag="VQ846_000073"
     CDS             68853..70607
                     /locus_tag="VQ846_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3074742.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAALTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            70591..72312
                     /locus_tag="VQ846_000074"
     CDS             70591..72312
                     /locus_tag="VQ846_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3074743.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILINGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            72323..72907
                     /locus_tag="VQ846_000075"
     CDS             72323..72907
                     /locus_tag="VQ846_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET3074744.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            72907..73602
                     /locus_tag="VQ846_000076"
     CDS             72907..73602
                     /locus_tag="VQ846_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET3074745.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VVGLIVGGLIWLK"
     gene            73590..74954
                     /locus_tag="VQ846_000077"
     CDS             73590..74954
                     /locus_tag="VQ846_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3074746.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKTSPFVFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT        23234 a        16037 c        13688 g        22427 t
ORIGIN      
        1 acaacctcaa aacactgttt tgagcagcct gcggctagct tcctagtttg ctctttgatt
       61 ttcattgagt ataaacttct tttcacaaaa aggatatgaa accctctggt caagagtttc
      121 cccatcctat cttctattct ttattttgct tctacacgga caccttgggt atcaactctc
      181 aagtcatgca gttttccttt gaaaggttgc ttttccaatt ctgccttaat tgttggcatc
      241 ttgtcatgag aagccagaac cataactgtc ggcccagcac cagaaaggta ggttgcatag
      301 gccccatttt ctttggtcac ttgcttaatc atcgcaaatt ctcttaccaa gtcctgacga
      361 tagcgctcat ggaagaggtc tccctcgatt gcttgcccag cggtcaccat gtctcctgcc
      421 aacaaggcag caaccgctac attggcgata gaacttgcag caacagcttc cttataagac
      481 aattttttag gcaagacact acggctgtcg cgagtacgta attcatagtt tggaatgtaa
      541 gctagaaaat cacactctgg aaagtctgct acgatagcag agacttgccc ttcaacagaa
      601 cttgcaataa cgagattacc ataaatggct ggagccacat tgtcaggatg cccttcaatc
      661 ttggtcgcta actgcaattt ttcatggtct gataagttga gttgacccag ttggttggct
      721 agttcaatcc cagcaacgat aaccgagctg gaagaaccca aaccgcgcgc caaagggaca
      781 tcactggtca ttttcaagcg tcttggttgc aagtctggta caatttgcaa agcgattttg
      841 agcaagagat tacgctcatc atgtggaatc catttgccaa tctggtgttc aatcagccac
      901 tcatctcgtt cttcgcagac ctcaatttga agatacttgg ttacagctac accgaccgag
      961 tcaaaacctg gcccgatatt ggcactggtt gcaggtacaa taatcttcat cttattctcc
     1021 tagcaccttg aaggtattca agaggtcgaa ttctgaaacc ttcttcaatt cagctgagac
     1081 attttcaagc tgggctttat taatcttgtg tgtgatgata acgacacgcg ccttgtcacc
     1141 ctctttgcca tcttgaagga tttgcttaaa ggaaatatct tgagcattga agatttcagc
     1201 caacttcaag acctgacctt ttgagtctag agccaagatt gagaaatagt agtttgcttt
     1261 gacatcttca ggatttgcca agaccaagtc acggctatat tcgttgaagt ctttgccaat
     1321 agtaccatca ttcaaacgac gaacgatacg gacaatatca gctacaacac ttgttgcagt
     1381 tggtttttga ccagcacctg gtccgtagta catagactca ccaataccga tagattctac
     1441 aaagacagcg ttcattacgc cattcacact agcaagtggg tgcgctttag gtaggaaggt
     1501 tggagtcact tctgcagcaa tacctgaaga agtttcctca atagaaccaa ccaatttcac
     1561 tacgtaacca agctcttgag ctacagctac gtcttctggt gtgatattgc ggattccctt
     1621 gtgggctaca tcatcaaagg caatcttcat gccaaaggca aattggctca aaataaccat
     1681 cttgtaggct gcatcaatcc catctacgtc attcgtcgga tcgctttctg caaatcctag
     1741 acgttgtgct tccgcaagag catcatcgta agaccagcct tcttccacca tcttggtcac
     1801 catgaagttg gaagttccgt tgactactcc aagcacgcgc gtaattttat cagaagccaa
     1861 ggaatttgct aaagtacgaa gaattggaat cccaccagca actgctgctt cgtagtaaag
     1921 tgctaccttg ttagcttgag cgatttctag caattctgcg ccatggacag ctaaaaggtc
     1981 cttgttagca gtaacaacgt gttttccagc ttccaaggca cgagtgataa aggttttagc
     2041 aggctcaata cgccccatca attccactac gatagtaata tcctggtctg ataaaatatc
     2101 atccacattg gttacaaagt taaagtcatt ccctgctgca agcaagcgat ttttttcatc
     2161 ttcatccttg accaatacct tagcaacttt gatatctgaa tgtgctgatt gattaatttt
     2221 tcctccattt tcctttagga ggaaaggcac accacttgca acggtaccaa atcctagtaa
     2281 agcaatttta actgtcatct ttatctcctc gaaattttct aatatagcca ttataacaga
     2341 attttgtgaa aattcctatt atagtaaatc actatttcag tataaaaaga aaaaacgaat
     2401 cagacgattc gctcttctta aaatctgaaa atagctttcc agaaaggatt agccgatttt
     2461 ttgcagattg agcactgcat cgtgactcat caagacttga ccatactctt gtaagactga
     2521 gcgactgata tcactatcgt ctgcaaactc gcgcatacgg gccaacagcc aagctggata
     2581 tgggcttgga tgattttcaa tatccactaa aatggtcaaa taatagcgct cgttcatttt
     2641 gtagagttca gaagtttcca tttcaaaagt cactgtcttg gcaaaagcta ccaagtcagc
     2701 caacttagca aaagaaagga tgtagtagat gtaaggttct ttcttactct cagcttcttg
     2761 ttcagcctgc tcttgctctt cttccttggc ttcaacttgc tcaagagatt gaatggcttc
     2821 gatatcatcc ttggttttgt ctgctatgct tttttccagg gttttgataa attcatctgg
     2881 agacatttga gccaattctt ccatatctgg caaatccgat aagtcttcaa aatctagatt
     2941 ttggtcaatc tttgacttgg tcacaaagac atctacctta tcaggttttg gagtcacacg
     3001 gaagctcaac atgcctgtat ccagaaagct atcaggcatc tctagctcat ccaagatagc
     3061 ataaaagaac tcttctgttt tttcttgagg aacgagaaag tcagcaatct ccattccacg
     3121 atccatcaaa tcctctaaag acatcgtgat ttttaaagtt gtatcactaa tttgtttcat
     3181 tttcattgct agtaacctca tactttcagt tctatctatt atactagatt tttacgattt
     3241 tatcaaaaga aggctcctct atacggatag attttcccta gggtctttct ataggagact
     3301 ccaaaagaaa atttctgcag acagatagaa aaagccttca aaatcggctc ttagccgatt
     3361 ttgaagacct tatacatcag aatacttata atttaaaggt tgctacaccg aggatagaac
     3421 gatttaagtt tctgagaatt tgaagacttt gctcaaattt cttataacga gtcactccgt
     3481 actcttcaac aagaaggact gtatctcttt ccaaaagaga tgatacatcc tgtaaatcta
     3541 caaaatgcat tccttttaaa gcttcttgac tctgtttcaa tttatctagg atagctttat
     3601 ttgagctaac gatggtcaat tcctgtccag tatttttgta tgacaaaaca tctgctaggt
     3661 tagcaattgt tgtaatctct gttacaaaat caatttgata ctgagaaaaa tcacctactc
     3721 tattgattgt tggattaaag agataaacta acacatttcc catcacaacc aaaatcacac
     3781 aaaccactcc aataacaact aaacgaagaa tcagattttt cacatttaag ccaagcgctg
     3841 tttcaccatt tgcgttcaat tctttagagt tgatggtttc cagtttttca attttcacat
     3901 ttgcataggc atgtttaaat ttctcgatca acccatcaat ttttttctct aacaagtcat
     3961 tggcatcttt acttgatgtc aaaattttca caccaacccc tgcatcgtca atcatatagt
     4021 agacggtcaa ttttttccac caatagtcat tcgttgaatt tttcaaggtt gtttctgtcg
     4081 tgtctaattc actggcaatt tttttcaact cactgggttc tacatcattg aaaagataag
     4141 ctccattcaa attaccatca atcaatttcc cataaaaatc actataacca ccaatttgat
     4201 gattcaaaat cgttttggcc gactcttttg gaggagtgat tttatagata agataagttg
     4261 aataacttgt tgtatctttg acagtgtttt tattcctaac tgctttaatt gtaaatggta
     4321 cagcaatgag agcaaataaa gcgatgagag ctaaaatatt tgcttttcgc tttttataaa
     4381 gatttgcaaa caaatcagct actgaataat gttcaaacat gatttttttc tcctttgttt
     4441 agtagatact agttttcctt tgtaagcatt tttgctacaa atataatcac aagaacaatt
     4501 ccccagaatt gcattgtaaa taaattgaag aaactttctg aaaagctgct tcttggcata
     4561 aagaatagat tattcaagat gagtagggat aaagcaaata ggattgtcct tgagcgatag
     4621 gctacttgca gcatggctat aaataatacg ccgagtaaga aactaagcag aaagactcca
     4681 atcataccat agtcggtata caactccatg atataactac ttccgatacc atgccctttc
     4741 aagtattcct tgttcaagac aagataggat agattgtggg cataactatt actatcaata
     4801 gctagttcca cactattggt tgtatgttca aaggcttttc ctccgaaaat ggctcccaaa
     4861 ctcccccttg caaaataatc aagaacagga ccaaaagtaa aattacggaa atctcggtaa
     4921 gggaggctac tgttaaatag aaaacctcga gccagaacac caaaactagt tccttgttta
     4981 tagataaagt caagtaagat atcccagaaa cctgtatggg aaacttggac attatcccgt
     5041 acataattaa gtactcccat cgctaacatg agaataggag aacctacaaa aatcgctaac
     5101 ttttctttaa acccaatcca ttttcctttt tcagtttgct cccgcataaa gtaataaaca
     5161 aaagcaaata aaatacttaa aataaaggga tttcgtgtcc caattgccaa atgaatagta
     5221 ttagctgcaa taaaggagac aagcactgct gtggcctgca atttctttgg cttggttgcc
     5281 agatacatac acattgcata gaccgtaaag gtagacaaaa tgtaggtaaa ataaggcagt
     5341 ttactttcaa aatttgcata gtaggcatag taggaagtct gcaaacgata caagagccgt
     5401 tcaaataacc gaatgaaata gaaaggatag gttagaagaa aaactcctag tgatacaaag
     5461 cgtaaccgct tgatataaac ctcttttaga gaatttccta tatttgctac ttttattttc
     5521 ttcctagcta tgaagtaacg agccaggacg cctcctgtgg tcaagcccag aatcgaaacc
     5581 atgacaacta taaaggcaaa acgataggct attggatgat aggtatccaa agcaccatcc
     5641 ctaaaataat caatggtcgg tcttgatacc agaaatacaa aaatggttaa atagaaaata
     5701 aaatggatta agtaatactt gatatcattc caacaagcaa ttaagctact aaccaacaag
     5761 aacaataaag tagaaagtaa gctaacatta ttattattaa acagatacac aattccactt
     5821 actagcgtca aggtataact gactatggtc aaactaaata ataatcgttt cccatcaatc
     5881 acttggtcac ccccgttcta atgtaatttt ttagattttt caatattttt cagtaataag
     5941 aatcggtata aggaaatatt tatgaatagg gccaaagcac taattcttct ccccttacgg
     6001 aaaattggat tcctagaaat agcaaaagca tagcctttta aaaaacgatg aatctgagaa
     6061 taggcttcaa actgtttata ctgatcatct agcaacatct tatccagaat aaagaagtga
     6121 gcataggcca atctgaaaaa agcgacctct ttcaagtcag gatagttttt cacaacttca
     6181 ttataaaact tttggtagat atcaatatag gctaaatcct tctttgcata gggtttggtc
     6241 gtaatactat cccctctatg gaaatagtaa taatagggtt tagtattaac cacatacttc
     6301 ttggccaact tgattaaatc aaaatggtaa taggcatctt cgtaaatcaa ccccttagga
     6361 aaggataggg cagttgcaat ttgtctcttg attagcttat tgcaaatcgt cccaggtatt
     6421 ttttcaccta tgaggtattc ctttagaaat gtttgagaat cacagacaaa atagtcatcc
     6481 tgattggctg actgtgggct ttcatcatta gcatagacat tcatgacacc acagcttgaa
     6541 acatccgcat cttcttgaac taattgctca tataagctct gaatcatttc tggatggata
     6601 taatcatctg agtcaataaa aatcagataa tccccgtgag cctgcttcat cccatcattt
     6661 cgtgcttgcg acaatccttc gttcttttta tgaagcactg acaccctgtc atcttgttca
     6721 gcgattgaat cacacaagcg accactttca tctgttgcac catcatcaac aagaataatt
     6781 tccagatttt gataggtctg cttctgaatg gaagctatcg atttttctag gtactgcgcc
     6841 acattataga ctggcacaat cacactaatt aatgcagttt ccatgctact cctctaatag
     6901 tttttctact tgttcgattt gttttgtaat tgtaaattgt tgaatgaatt ggctagcctc
     6961 atcgacatta aagtttgagg cagaagtcat gtaattagta atcgcctgag ctgcctcttg
     7021 attgctctca atgatttgtc caaatcgtcc ttcttgggat aattcctcag cccctccaac
     7081 gtccgtagag ataaaaggga gtcccagact caaggcctcc acatacactc caggaaagcc
     7141 ttcttgttta gacatagaca agagaacttt cgtctgagat aaatactgat aaggattttt
     7201 ttgataacca aggaaatgta catagtcctc aatctcatac tctttgactc gttttttcag
     7261 ttcctcttcc atatcaccag ccccgataaa atagagatga tagttttttc cctcttggtg
     7321 taataatcgt atcacttcca ctacacggtc agaaccctta ttttcctcaa tccgtccgat
     7381 agtacagata ctttgaggag caatctcgat atcgatcttc tcttgagatt tttctagaat
     7441 agtctgaaaa tcatatccat tgtagattgt ctgtaattta gaagcataat ctggataaac
     7501 ttccttgata gaattgctgg tcttttttga aatccctaca attgtattcg cagcatccaa
     7561 ctggcttcta tgtgattctc ttttagagct atccttaaga agttcttcaa tacttccatg
     7621 aatccaagat atcttcttga cttctcttct tttagagaac aacagtggtg gattcataat
     7681 ggtaaaagaa acttcaacat cataatcatc ttttacaagc aaacgacgag tcagtcttgg
     7741 aaaataaatt ctcattctcc acaaaaaagc tcgtaaccat ctggtttggc gataatcttg
     7801 aagggatttt aaaatgcgta catgctttgg aacagattca tatcccttgt caaagtgctc
     7861 catttcaaga atatcaatat catacttttc tggatccaga tttgaaacaa tggttgatag
     7921 aatcttctct gcaccacctc caagagaaaa agaccacata aaaaataaga tttttttctt
     7981 agccaccata ttctcccttg tattctgtat aagacttatc catatcagcg atgacagcat
     8041 catgatgcgg taccttcttg tctgctggtg gaggcgtcat ataatcccca aaagcagttc
     8101 tgagatagac atcatagccg attggaatag gcatctctgt tccttcaaat ggcaagaaaa
     8161 gattgtcttc aaaagacgtg attgggtact tgtttctcat gtagccagga cctgagcata
     8221 attctgtaat gccatcacaa tcagccaaat catacttagt catttctttc tcagcttttt
     8281 tccagatgcg ataacggaga gattttggag tcaaacccag taaaatgcga cttccccatt
     8341 tcatgagagc accatgcttt tctggaatag tttgcgcaca aaagagtgaa taaatcaagg
     8401 cccaacgaac ctgttttttc cgctcagctg gatttttcgg ataataatcc aaaggcaaaa
     8461 catccaaggc cagaccatgt ggcaaatcca aatcctgctg ataaggcttg atacaggtgg
     8521 tttccttgtc acgaatggta ataaaaagat tacgatcaac aaaatccttg tgactctttg
     8581 acaagaaata acgttcatct gcataacgag gccataattc tgctaatttc tcataatctt
     8641 tacgaggcat aaaaaagtct aggtcgtcgt cccaaggaat aaatcccttg tttcgaaggg
     8701 caccaatagc gcctccgcca cagagataac agagcaaatc atgttcttta caaaaggcca
     8761 caaaatattc agccatctcc agactacgag cctgaattgc ttttaaatca gtcatattgt
     8821 tcattattct ttctatcgta tcgtttcatt ataccacaaa caaggggtga aaatctattg
     8881 cagactgtaa aaaatcaaag cctgactgct atccaaatag ctatcaaact ttgatttttc
     8941 tgtcttatac tcttcgaaaa tctcttcaaa ccacgtcagc ttcaccttgc cgtaggtata
     9001 ggtaactgac ttcgtcagtc ttatctacaa cctcaaaact gtgtttttag cagcctgcgg
     9061 ctagcttcct agtttgcact ttgattttca ttgagtatta tcttatctca agcccatttg
     9121 agcgagcttg gtttgatatt tgttttgatc aaccagcaag cccaagcctc cataaacatc
     9181 ataggcatct acccagtcac ccagttctgg aatcgtcaat ttttcaatac cattttttgc
     9241 tccatccaaa acagataaac cgtttgttag gaggaaagta tagggtacgt tggttgaggt
     9301 catagcaaaa acctttccaa gagcttcaga accagtgaaa agtttagtgg gatctttaat
     9361 ttgctctaaa attgctgtta aaacttgttg ctgtcttttt gtacggccgt aatccgcctc
     9421 atcatcatca cggaaacgag cataattgag cagggtcgag ccattcatct gctgttttcc
     9481 gactttaatg gtttgggttg gagactcagt ctcggtagcg tataaatcat ctccgactgt
     9541 agcttctgtt aggggacgcc cattcaatgt tgaaaattga gcatcaatcg tcaccccatc
     9601 agggaaaagc gtgtcaatcg ctgtggcaaa ggcctggaaa tcaaccaagg cgtagtactt
     9661 aatgtccaag tcaaaattat ctttcaagac ttggcgaacc atttctgccc ctttttgccc
     9721 ctcttgttct cctaactcgt aggctacgtt taacttgtta tctgtctgtt ttctaccgtt
     9781 aatcacttga ctataaccat ctatatagac caaattatca cgcatgaaac tgactagctt
     9841 cattttctta tctgagcccc cgacatttaa taccataata gagtcagttc gtgtctcaac
     9901 actgttctgg ccgattcgac catcagtacc catgattaaa atattaactc catctctagt
     9961 gtcctgacca ttaaagactt ctacttgagc tgcccgggca tcagcagttt tctttgcgct
    10021 agcatcttgg taaccacgca aaaacatgaa taccatggcc aaagccacac agaccaaaag
    10081 tgaaaaaatc accataaaaa ttcgtttaag acggagcttc cgtcttttct tttttggagg
    10141 gaaagagagt gcttgtgatt tggattgtga gcgactccgg ttcgcatagc ttggtaagtc
    10201 aacctgctct tctctttctt gttccaagct agagctacta tttcccctag caagagttag
    10261 cttttcttgc aaataggcaa actcattttt ttctctctca ttgagatagt gaatattttt
    10321 tagcaaataa tcataacgca actgctcatg atgacttaag ggattttctt tactcatctt
    10381 ctctcctttc catggtctga tattggataa ataggatagg cacccagaat tttatactgg
    10441 attccaatcg cttctaattc tttttgggca aagtggacca agtccttatc ggtataatcc
    10501 acatcgataa tgaaaaagta ttcacccagt gctgtcttga gtggacgact ttcaattttt
    10561 gtcaagtcaa ttcctcgcca agcaaaggtc gacagggcct tataaagtgc acctggaagg
    10621 ttgtcaggta atgtcaaggc caaactcatc ttttcagttt gtgcttgcaa gggaatacta
    10681 ggcttttcag ctcctagaac ccagaaacgt gtgaaattgg cttccatttc ctgaatatcc
    10741 tcggcaatca gttccaatcc atattcttca gcagaacttc taggtgcaac tgctgcaaag
    10801 ggctggtctg gatgttcgga aataaaacgg gccgcataag ctgtactagc tgttacctcg
    10861 atttgagcct ctggatattg ttcatcgatg aatttctttc cttgagccaa ggcctgtgga
    10921 tgtgaaaaaa tcttttcaat cttagtatgg cctggaacca ccatcaactg ctgatgaata
    10981 ggctgaacga tttctgctac tgcttggatg tgagcctgat gaaaaagata gtccaaggtt
    11041 tcatgaacac taccctcaat agaattttca actggcacca cagaatagtc cactaatcct
    11101 tgctcataag ccttgatgac atctgtaatg ttagcaaaag cctgcaattc ctcatgagga
    11161 aaagctatct gcacaacgtg gtgtgaaaat gatcccttgg gacctagata agcaattttc
    11221 atcttagttc ctctataatt tcctctgggc ttagcttggt cacatccaaa acccgactag
    11281 ccacttcctc ataccaagcc tgtctttctt gaaaaatagc tactagttct tccttgctat
    11341 tatttagaaa aagcggtcgc tgattgtcct tatcagctgc gatacgttgg tagagggttt
    11401 caaaatctgc tttcaggtag atgttatctg tattagtctt gagtaagtca cgatttctct
    11461 gagaaataac cactcctcct ccagttgaca cgacttggtc tgtttgtagt aaatcagcta
    11521 ggacttctga ttctacctga cgaaaggctg cttctccctt ttcagcgaaa aaattcgcaa
    11581 tggacatacc taggcgcttc tcaatcagag catccatatc aaggtaatta gggtccaagc
    11641 ctcttgcaat agtcgatttt ccagccccca taaaccctaa taacacctta gccatgaatc
    11701 aagctctcca aatcatcaaa gaaactagga tagctggtat tgatggcttc tgcacggtca
    11761 agctccacct ctccatctgc aaccaagagg gctgcgatag ctgtcatcat gccgatacgg
    11821 tggtcaccaa acgtattgac tctagcaccg tgaagagctg atttcccttt gataatcatc
    11881 ccatctgccg taggagtaat atctgctccc atactattta aggcgtctgc cacaacctga
    11941 atacggtctg tttccttgac cttgagctcc tcagcatcct tgataactgt tacaccttgg
    12001 gcttgagtcg caagcagggc aataatgggc aattcatcaa tcaatcgtgg aatcaaagcg
    12061 ccaccaatct ctgttccttt caagtcagaa gactcaacag tcaaggtaga agatttagcg
    12121 actggatcga tttcagttat ttccaatttt ccacccatgg cacgaatgac atcaataata
    12181 ccggtgcgag tttcgttgat ccccacattc tgcagcacta gacgagaatt tggaacaatc
    12241 aaacctgcga ctaaccaaaa ggctgcactg gaaatatctc ctggtacgac caccttctgt
    12301 cctgtcaatt tttgtggccc ttggactgtg attttcttac cgtccacact taaatgacca
    12361 ccaaattgtt tcaacatatc ttcagtatga ttacgggtgc actctttttc gataataact
    12421 gactccccct tagcttgtaa ggctgcaaac atcaaggctg acttgacttg ggcagaggca
    12481 attggcaact cataatgaat aggtcttagg tttttcgtcc cttttaagcg aaggggaggc
    12541 aagtctcgtt cagtttgccc tgaaatgctg acgcccattt ttttcagtgg aagggtcaca
    12601 cggtccatag gacgtttgga aagactatca tctccaagca tctctacttc gaaatctgca
    12661 ccagcaagga cacctgaaat caggcgaatc gaggtgccag aatttcccat attaagggca
    12721 ttttgtggcg cttttaagcc agccatgcct acaccttgaa tggtaataac cccatcttta
    12781 tcctcaattt caacaccaag gtcacgaaaa acctgcatgg tcgaaagaac gtcttcacct
    12841 cgcagaatat cataaacctt ggtctcaccc tcagccaaac ttccaaagat aatggaacgg
    12901 tggctgatag acttgtcacc tgggacgcgg atactaccat gtaaatggcg aatgtttgtt
    12961 tttagtttca tactggacct catacttgca atacttttac ctattttatc ataaaaagcc
    13021 agaaattcct taaaaattcc tgactttagg atcgttcttt tcttatttca gcaattctga
    13081 aactggttca aaaacaattt tttcaatgtc agaaaggtaa atggccaatt gttgttgctt
    13141 ggtaaagaat tctgacaaga ggctatttcc ttgaatctgt ttaccaaagc cttccatctt
    13201 agcttggaag gacgcatctg gcatttgacc tgtatgtgct agtttttgaa tttcctcttg
    13261 aaaggcaaga tattctgtaa agattttgct tgcctcagca tctgctgcaa tcgcatcttt
    13321 agctgcttta acagccttgt attctggtaa tccgcgtaga ccgcgactga gttcgtttgc
    13381 actatcgtaa atatttgaca tgttcttctc cttatttgat gacgactgta tagtcggtat
    13441 tttctgttat gagatgctca gctctttcca agtcttgagc atttttaaat gaaatttgta
    13501 ggattccgtg aatatcctca cgattttcct cgttgatgtg gatgttaacc aaggaagtcc
    13561 cacgtagcag ttccaaaatc cgcaggatga catcttcttc atcaggaacg tcaacataga
    13621 ggtcgtaaga gctatccaca ccaccacgct tatggatttc catggtctgg cgttgttcac
    13681 gcgcttggtt aaaaaagttc caaatttgct cttcatctcc cttactaatg gcctgaccaa
    13741 tcacttccaa acgttccttg aaatcctcaa ttctatccag aatggtctcg ctattggaca
    13801 agagaatgga ggtccacatt cctggctcgc tttccgcaat tcgggtcata tctcgaaaac
    13861 cacctgccgc aaagcgcctt gccatctcat gctcttgagc atagaccgca gtctgctcca
    13921 tgagactaga agccaaaata tgaggaaaat ggctaatctg agaagtgaca cgatcatgct
    13981 ccttggcatc aatctcgata aaacgagcat gaagacctga aagcagatcc ttcatttcct
    14041 taagcgtgtc ctgacttgtc aggcttgaag gtgtaaagat ataataggca ttttcaaaaa
    14101 gattgacatc tgccgaagca gcccctgtct tgtgactacc agccatggga tgggccccga
    14161 caaagcgaac agacttgcca gccaaatact gctccgccgc atccacaatg gttgacttgg
    14221 tcgaaccagc atctgaaata ataacgcctt ctcgcaaatc caaattggcc aactccttaa
    14281 tgaaagcaat agtttgtttg attggcaagc tgaggataat gacatctgcc aaaggagcaa
    14341 aactagcaaa atcatccgtt gcacggtcaa tcatgccttc tttcaaggcg atatctctcg
    14401 aagcttgact acgattataa cctaaaattt cataatctgg atgatcgcgt ttgataccaa
    14461 gtgccataga ggctccaatc aacccaagac ctgcgatata gattgttttt gccataggaa
    14521 ctccttaata gttctttgta tagtctcggt gtttggctac cgcttctttt agttcctcaa
    14581 gattatctga tgagaatttt tcgaggattt cttgcgccag aaccgttgct acaactgctt
    14641 ccatgaccat tcctgcagct ggaagagcag tcggatcact tctctccacg gttgccttgt
    14701 aaggttcgtg ggtttcgata tccacactca taagaggttt ataaagagta ggaatgggtt
    14761 tcatgacccc acgaacaacg atgggttgcc cattagtcat accaccttca aaaccaccta
    14821 gattattggt acggcgagta taaccgtctt ctttagacca gagaatttca tccataactt
    14881 ggctgccttt acgataacca gcctcaaagc caagaccaaa ttccacccct ttaaaggcat
    14941 tgatagagac aacagcttga gccaatcttg catccaattt tctatcccat tggacatagg
    15001 aaccaagacc aactggaacg cctccgacga ctgtctccac aaccccaccg atggtatcac
    15061 catcacgttt gatttggtca atatagtcct tgatttcctg ttctcgttct tggttaacaa
    15121 tagaaacttc agactgggca gctctttgct taatttcagc gactgtcaga ttttcaggaa
    15181 catcgatttc cttgccacca aagaccacga catggttggc aatctccata tccagctcag
    15241 ccaagaggcg tttggctact gcaccaactg ccacccgcat ggtggtttca cgagctgatg
    15301 aacgctccaa agaatttcgc aaatcatcaa aacggtactt aatcccccca accaaatcgg
    15361 catgacctgg gcgaggatga gtaattttcc gcttgctttt aaggcggtct tcaatgtcct
    15421 ccgcagacat gatgtccagc catttctggt ggtccttatt gatgacatcc atagtaatag
    15481 gcgcccctgt cgtcttcccg tggcgaacgc ccgaagtaaa gacaacctgg tcactctcaa
    15541 tcttcatacg accaccacga ccgtagccac cctgacggcg tctaaggtcc tcattgatat
    15601 cctcagctgt caatggaagt ccagctggaa ttccctcaat aatagctgtt agacgggggc
    15661 cgtgtgattc tcctgcagtt aaatatctca tacactctcc ttattttacc aagtagtctt
    15721 tcatctcttc cagagaaact gggtgaatga tcgctgaacc aagctctggc accaagacca
    15781 atttcaaggt gttaccacgc gctttcttgt catgagtaag agcctgataa agcttgtcaa
    15841 cttcccaatt ttcatagtca acaggcaagc cgaatttctg acacatctct gtgatagatt
    15901 gggtaatgcc agctggcatg aggccttttt cctcagcaac cttggaaatc tgtaccattc
    15961 ccatggcaac agcctctcca tgcatgacct tgccataacc ggcagtcgct tcgatggcat
    16021 ggccaatagt gtggccaaaa ttgaggtaaa gacgaatacc attgtccaac tcatcttcaa
    16081 ccaccatctt gcgcttcacc tgacaagaat gttcaatcaa ggtctctgca tgttccaaaa
    16141 tactctcaac agaaccattc agtcccgtca agagagccca cagttctgga tcctcaatca
    16201 agccatactt gataacttca cccatccctt caatcaactc tctttttccg agggtttcaa
    16261 gaacaagtgg atcaatcaga accccatctg gttgggcaaa ggtccccacc atatttttag
    16321 caaatggtgt gttaacgcct gtctttccac cgatagaaga atcaacctga gctgtcaaac
    16381 tagtcggaat ctgaacaaag tgaatacccc gcatataggt agaggctaca aatccagcca
    16441 ggtccccaac aacgccacca ccaagagcaa cgattccatc gctacgagtc agaccttgct
    16501 tgactagaaa ttcatagact ttctgaacag tagttaaatt ctttctttct tcaccttcta
    16561 agaaatcaaa aacagctacc tgaaaaccag catcttctag gctgagcttg accttctctg
    16621 catagagaga ggctacatgg ttatctgtca caatgactac cttttgcggt tgccagagtt
    16681 ctcgcaacca ctgaccagcc tgggccatac aacctttttc aatctgaata tcataaggat
    16741 gatgaggaat atcgattctg attttcatag gagagtttcc ctttctttat tggtattttt
    16801 ctgttaaaga ctgccaaatc tcttctgtcg gcatttcctt gcctgtccac agttgaaaag
    16861 cttctgcagc ttgatagagt aacattccca gaccattgac tgctggattg ccctgacttc
    16921 tagcccattt caaaaacggt gtttcaaagg gttggtatat gatatctgca actaaaagag
    16981 tttctggtaa gactatgttt tcaggaacag gagaggattg gccatccatg cccacactgg
    17041 tggcattaac tagcaaatcc gactcggcaa tccttgcttg cagttcagaa acatattcta
    17101 aagcacacaa atccacttta aaacctgtct gctcctgtaa cttgtctagg taaggtcttg
    17161 ttttttccat agaaacggaa cgaacaaaga ccgaaatctg actgacgcca tccaaaatag
    17221 cctgtgccaa gattgattta gccgcaccac ctgcacccag cagggtcatc tttttacctg
    17281 aaattgtaaa agaaggcaag cacttaaaaa atcccttgcc atctgtatta tatccaatta
    17341 aattgccatt ctcattgaca accgtattaa ccgcaccaat caagcgcgct tcatcgctca
    17401 gcttatccaa ataaggaatc acctgctcct tatagggcat ggacagattg atgccaaaca
    17461 tctggtagcg acgaatattg gccactgttt ctaccaagtc actcgcttca atctcccaag
    17521 ccacataagc accgttggta tctgtcgcct caaaggctct attgtggatg aagggagaaa
    17581 tagaatgctt aataggattg gcaacaactg cagctaaacg tgtatagcca tcaagcttca
    17641 tccaaaatct ccctgatttt tttcatgcta gctagagaaa tctgcccagg ggcactaacc
    17701 tcatccagac tggcaaaaga ccaactcgaa ccagtcacat ccgcagtgat acgagagacc
    17761 ttgcccacct tacccataga aatggtcaca tattcctgtt caggattgag ggttttaaag
    17821 cctcgtgtat agttcatcaa gtctaagaca tcctgctccg tgtgagccat caccgcaacc
    17881 ttaacaagtt ttggatttag gatcgtcaac tctgacaaga tttccatcat gttctcaggt
    17941 gtttcttgga aattatggta actcaaaaca agatttggga agtccagcat ttcctcaaaa
    18001 acatccttgt agctatagta ctcaaaatca atatagtctg gttgatagag ttgcgcaact
    18061 tccttgatta gatggatata ctcttctgga gaaaggtcga tttctccacc ttcggagcga
    18121 gttcgtagcg tgaaaaccaa ctcacggcct gcgaattttt caaaaatggc tggagctacc
    18181 tgcaaaatcg cttctttagg cagatagtcg gcacgccatt caatgatgtc ggcatccagg
    18241 tacctcgtgt catccagagc ctgagcctcc tctaaacttc ttggcattac tgaaacgatt
    18301 aatttcattt actaaccttc atactaatca ccttgaggta attactactt tcatcttttt
    18361 tattataggc aaaatctgct ggaagaccat atttgtttaa aatctggtaa cttcttcctg
    18421 caaaaccttt atcaatttgt tctgtaaatt tctgacggga aacattggca gcattggtac
    18481 tggcaatgat aatccctccc ggatttaaaa tctcaagact ctgggaaatc aacttgtgat
    18541 aatccttggc cacagagaaa gtttgttttt tattccgagc aaagctaggc ggatctagga
    18601 caatcacatc gtaggtcaag tctttgcgtt tggcatattt gaaatactca aagacatcca
    18661 tgactataaa atgatgctcg tctgtgctga gcccatttgc ctgaaaatgc gcttgagaca
    18721 attctcgtga acgtttggct agatcaacag aagttgtctg gctagctcct cccatggccg
    18781 cagctactga aaaagccgct gtgtaggaaa acatattgag caaggattta cccatagcca
    18841 agccgtcaac taaactaccg cgaacctcat gctggtctag gaaaatgcct gtcatcaagc
    18901 catcattcat aaagacttga tacaggacac cattttctaa aacagtgaaa aagtcaggtg
    18961 cttcttgacc ataaacatgg gcagattcat agtccaaacc cttaaagcgg attttctcat
    19021 aagctcctaa aacctcaggg aaaacctgtc taaaggcttc tgatatagtc tgacgaatct
    19081 gataaacata agagttatac caagaaaaga cggcgtagtc gccataaagg tccactgtca
    19141 gacctccaaa gccatctccc tcttgattaa agagacgaaa ggcagttgtc aaatcatctt
    19201 gatagtaggc gtttctcttt tctttggctt ttctaaacaa cgtttcaaag aaagtttgat
    19261 tgaaggccac cttgtctttg ctgacaaacc agcccaagcc cttgttttgc tgagaaaggt
    19321 aggcagtccc aagaaagttt ccttcctgac cctgcacctc tacttcctga tccttaagat
    19381 tgacattctc aagatcactg gcttctagta aaactagccc cttagcaagc ttcttttcaa
    19441 cccttttgct gactcttatt ctattcataa ctaccattat atcaaacttt tagacaattc
    19501 tcaaaaaaga aactaccctt gcttttttac tcttctttta aaaaatggta tactagactt
    19561 cctgcaaaac taggaagtaa atgtgtaaga atcacaataa aaaatgctct tccgtcttgg
    19621 aggagcattt ctttttatca acgaaaatca aatagcaaac tatgaaacta gcctcaggtt
    19681 aactgtgaga ttataggtag agaggttgaa tcagcaatat gtgtctgtca aatttagtga
    19741 caaaggtagt agaagaaaga tgaagaaata aatctgcttc aataggtatc tggaaaattt
    19801 gattttatag agaagccttt tgttacaaac tcaatatact atcaataaat aatattatag
    19861 aagcaacaat aattataatt tcacctatct gcatcattct atttcgaact ctaaatatat
    19921 gttctatcaa aaatacttgg atcacacaca ttataggaat taacgttttt gaaattgaaa
    19981 aatatcccaa taaataaact ataaacaaca aaaatagaac tatgttatat ttcttattca
    20041 aaacattcct ccctatatat ttttgattac caatcttaat catttacaac tacattctaa
    20101 caaactataa aagcgtttgt cgaactgaat ttatcaagca agcgaccaac cagctcatct
    20161 tttttctatt tctgccaata tgcgtgacag gtaataatga tagccaaaaa tagcaagagc
    20221 aagcaggagg ataagagctc ctactcccaa gctgatggca aggatagggg agagagactg
    20281 aaccaagaat atactcccaa ttacaagggc catcaggatt gcactataaa taaacaataa
    20341 aactatggcg actatgccat ttgaacgatt caccaggtcc gtaatgctac tccaattggt
    20401 tgacagattt ttcacatcct taaaataatg gtgacaagaa aggatgacac tggcaaggat
    20461 ccatgccaca ataaggtaaa tcatcgaaat gatgggcaag cctagatata gagaaagacc
    20521 aagtaaagtc agaattggta aaaaggactg aacagcaaag ataatccaaa atttcacttt
    20581 cacataacga gcaaagtcaa agggtaaact cttcagaaaa tcaacatttt ccctctccaa
    20641 ggacaaggca attgaaggca ggctggtgat attgttattg acaactgcta taaagagagc
    20701 tataaaaaac aagggtaacc agtatggagg atgaatgtct ggaactatct gagaatctcg
    20761 gattttggaa atcagaccga tcatcatgag ataaggaagg aaagcacttg taaaaagcac
    20821 tgtaaccacg ccagtcccct gtcccaagag ggtgaggtgg tagcgtaaaa ccatgcggaa
    20881 aaatcccttt ttagtggttg aaattctctc cttgctgcga cgttcttttt tgaccttctc
    20941 ctcactatta agcaggatca cgtcataaaa acgaggaagg accttctttt tggtcagata
    21001 aagcaggaag agagttagtc ctatccaagc gagcagaccc aatatggctt ctattgaaaa
    21061 aggctccact gctattttgt aaaagatatg aagaggataa aggagaaatg gaatgtctct
    21121 aactttgtca acaatacttc caaaagtcga ctgaagaaag aagataaata ttaaaggtat
    21181 gagaactcct atcccaatca tcacattcga aaaaatagac tgatactttc tgaagaccct
    21241 agtctgagcc aagaaatgta ctgccactac cgtcactaaa gtaacagaga caaataataa
    21301 ggtcaaggac agtagcatca aaggcaaacc cagccaaaga gaaggagcta gactaatata
    21361 gagggctaga aaataagcta ggattggtac aattccagtt agagctggca agaggacaga
    21421 cagtccttta gcaattataa tctctgattc tttaaaagca tagggactat aggaggccaa
    21481 atccttactc tcataaaaga cattgtaaaa tgcagttaaa gaagttgaaa aggcaatcac
    21541 tagtaaaata gcaaccatga aactaaaata aacaggcatt tcctcaaaag gaaaatgaat
    21601 ggctatagta ctaaaaaaga tgagcatcaa gagactggaa aaaatgtaag aacttaggac
    21661 tctagcggaa acatttactt tttttccagt atttttagct tgcttctttc gtgggttagc
    21721 cagattagcg tcttgagatg aatagaggat attgatatca actaattttt taatgacctt
    21781 gagacgcatc tgaaacctcc tcttttctac cagcaaggct aaggtagata ctttccaaag
    21841 actggtctgg gttgtctttt ctcaagtcct ctaccttacc acaataaatc aaatgcccct
    21901 ttttcaaaat agcaatccga tcacagactt gctcagctac ctctagcaca tgagttgaaa
    21961 acaaaactgt cttacctttt tgcgcatgct ccttcatcat ttgcttcaaa tcaaaggcgg
    22021 cctggggatc caaaccagtc aagggttcgt ccaaaaccca aatatcagga tcagacaaga
    22081 gtgccccgat gacaaagact ttctgacgca ttccgtgaga aagagtttca ataacctgat
    22141 agcgattttc agcaaaatca aaaacgttca atagcctagc tagactagcc tccaagtcag
    22201 atctactcag atcataggat gaggcgatca attcccaaaa ttcattggcc gttaagcgta
    22261 aaaataagtc aggcgagtct gctacgtagc caatctttcg tttaatagcc aagcgatttt
    22321 ccgataactc ctgaccgtct accaaaatac gaccactgct gggtgaaatg atactgacta
    22381 gggattttat agtggtcgat tttccagccc cattatgacc aatcaagccc ataatctctc
    22441 cattttcaat ctgcaaattg agattactca aggcctcttt atcaccatac aacttactaa
    22501 cattttgaaa ttcaatcatg ggacaactcc tatctttatc tgtattatat atcgctatac
    22561 tatcactttg atacaaaaaa tcaatacttt attttaagta gttaaaacag tttccttcta
    22621 ttcttacgca aacgtttgcg ctaaccaata ctttatgata gaataaagaa caagattgac
    22681 aagtaagagg aaaaatcatg caaaatcaaa cgctcatgca atactttgaa tggtatctgc
    22741 cccacgacgg tcagcactgg acgcgtctgg ctgaagatgc tccacaccta gctcatctgg
    22801 ggattagcca cgtctggatg ccaccagctt ttaaggcaac caatgaaaaa gatgtcggct
    22861 atggtgtcta tgacttattt gacctaggag agtttaacca aaaagggact gtccgcacta
    22921 agtatggttt caaagaagac tatcttcaag ccattcaagc cctaaaagca cagggaattc
    22981 aacctatggc cgatgtggtt ctcaatcaca aggctgcagc cgatcacatg gaagcctttc
    23041 aggttatcga agtggatcct gtagaccgta cggttgaact tggagaaccc ttcaccatca
    23101 atggctggac tagctttacc ttctatggtc gccaagatac ctacaatgac ttccactggc
    23161 attggtacca cttcaccggt acagactacg atgcaaaacg ccgtaagtct gggatttatc
    23221 tcatccaagg ggacaacaag ggctgggcta acgaggaatt ggtcgataac gaaaacggaa
    23281 actacgacta cctcatgtat gccgacctag actttaaaca tcctgaagtc atccaaaaca
    23341 tctatgactg ggctgattgg ttcatggaaa cgactggtgt agctggtttc cgtttggatg
    23401 ccgttaagca tattgactct ttctttatgc gcaacttcat ccgcgatatg aaggaaaaat
    23461 acggtgacga tttctatgtt tttggtgaat tttggaaccc agacaaggaa gccaatctgg
    23521 actatctcga aaaaacggaa gaacactttg accttgtcga tgttcgtctc caccagaatc
    23581 tctttgaagc cagtcaagct ggcgcaaact atgaccttcg tggcattttc acagatagcc
    23641 tggttgaact caagcctgac aagtctgtga cttttgtcga caaccacgat acccaacgag
    23701 gacaagccct tgagtctacc gttgaagaat ggttcaagcc agcagcctat gccctcattt
    23761 tgttacgcca agacggcctt ccatgtgtct tttacggaga ctactatggg atttcaggcc
    23821 agtatgctca acaagatttc aaagaaatcc ttgaccgcct cctagccatc cgaaaagatt
    23881 tggcctatgg agaacaaaat gactactttg accatgctaa ctgtatcggt tgggtacgtt
    23941 caggtgctga aaatcaatcc ccaatcgcag tcttgatttc aaatgaccaa gaaaacagca
    24001 agtcaatgtt tgtcggtcaa gaatggacta atcaaacctt tgtagattta cttggtaacc
    24061 accaaggtca agttacaatt gatgaggaag gttatggaca attccctgtc tcagctagat
    24121 ccgtaagtgt ctgggcagtc aataccatct aatagctcat aataaccaag ctaggtccaa
    24181 gcggatttgg cttttttgta gtcaaaaaag acctacccaa atggatagat ctttacttga
    24241 ttacaattta cctgctacta catccaataa ttcttggatc ttagcttggt tgcttcctcc
    24301 tgccatggcc atgtctggtt taccaccacc acgtccatcg atgattggtg ctaattcttt
    24361 gacaaggttt cctgcatgaa ggtcttttgt cttgcttgct acaaggacat tgactttgtc
    24421 accgatagcg gcaactagga caagaagatc agagtagtct ttttgtttcc agttatctgc
    24481 aaaagtacga agggcaccgg catcggatac agacacttga ctagcaatgt aacgatgacc
    24541 gttgacttcc ttagcatctt tgaagatatc gcctgcggct gcagctgcgg ctttttcttt
    24601 caactcagca ttttcttttt gaagttgacg aagttgttct tgaagtcctt ctaccttgtg
    24661 aggtacttcc ttgacttgag gtgctttcaa ggttgctgcg acagctttaa gagcatcctc
    24721 ttgttcacga taggcttcaa aggcttcctt accagtcact gccaagatac ggcgagttcc
    24781 tgaaccgatt ccttcttctt tgacaatttt gaagagacca atctcagaag tgttgccaac
    24841 atgagtacca ccacaaagtt caatagagta gtcaccgata gtcacgacac gaacttcctt
    24901 gccgtatttc tcaccaaaga gggccatagc tcccatttct ttagcagtgt caatatccgt
    24961 ttcaactgtc ttcacttcaa gtgcttccca aattttctcg ttaacttgct gttcaatcgc
    25021 acgaagttcc tcagcagtta ctgcttggaa gtgggtaaag tcaaagcgaa ggaattcaac
    25081 ttcgttaaga gatcctgcct gtgttgcgtg gtttccaagg atattgtgaa gggcagcgtg
    25141 aagcaaatga gtcgcagtgt ggtttttcat gacacggtga cggcgattgc tatcaattac
    25201 caaggtatat tcttggttca aggcaagcgg tgcaaggact tcaactgtat gaagggcttg
    25261 accatttggg gctttctgaa cattggtcac agtagccaca accttacctg actcatccaa
    25321 gatttgtccg tagtcagcta cctgtccacc catttcagca taaaatgacg tttccgcaaa
    25381 gataagagag gcagttcctt ctgaaacagc ttctacttct gcattgtccg ccacgatagc
    25441 taccaattta gaagacaatt ggctagcatt gtagttgaag acactttcta cagtgatgtt
    25501 ttgaagagtt tcattttgca tacccattga gccacccttg acagctgagg cacgcgcgcg
    25561 ttcttgctgt tctttcatgg ctgcttcaaa accttcacgg tctacagtca taccagcttc
    25621 ttcagcgatt tcttcagtca attcaactgg gaacccataa gtatcataga gtttgaagac
    25681 atctgaacca gcgataacag attgaccttt ttctttcaag tctgctacaa tgccttgggc
    25741 aaagtgttga cctgagtgaa gggtacgggc aaatgattct tcttcgctct taacgatttt
    25801 ctcaataaag tcacgtttct caagcacttc tgggtagtag ctttccatga tttttccaac
    25861 agttggaacc aatttgtaaa ggaaaggttc gttgataccc aatttttgac catgcataga
    25921 agcacgacgg agaagacgac gaagaacata accacgacct tcatttcctg gaagggcacc
    25981 atcaccgata gcaaatgaaa gagaacgaat gtggtctgcg ataaccttga agctcatgtt
    26041 gtcgccatct tggtcataaa ccttaccaga caatttctcg acttcacgga taatcggcat
    26101 gaagaggtcc gtttcaaagt tggtcttagc cccttggata acagccacca aacgctccaa
    26161 accagcgccc gtatcaatgt tcttatgtgg caattccttg tattcgctac gaggaacagc
    26221 agggtctgcg ttaaattgtg acaaaacgat gttccagatt tcaatataac ggtcgttttc
    26281 aatatcttct gcaagcaggc gaagaccgat attttctggg tcaaaggctt ccccacggtc
    26341 aaagaagatt tctgtatctg gtccagaagg tcccgcaccg atttcccaga agttgtcctc
    26401 aattggaatc aagtgacttg gatccactcc cacttcaatc cagcggttgt aagaatcttt
    26461 atcgtctgga tagtaggtca tgtaaagttt ttcagcaggg aaatcaaacc attcagggct
    26521 tgtcaaaagc tcataagccc aagtgatagc ttcgtcacgg aagtaatccc cgatagagaa
    26581 gttccccagc atttcaaaca tggtatggtg acgcgcggtc ttccctacgt tttcgatgtc
    26641 gttggtacgg atagcctttt gggcattggt aatacgtgga ttttcaggga taatggtccc
    26701 gtcaaagtat ttcttaaggg ttgctacccc agagttgatc cacaaaagag ttgggtcatt
    26761 tacaggaacc aaacttactg atggttctac tgagtgacct ttggtcgccc agaaatcaag
    26821 ccacatttgg cgtacttgtg cactagatag ttgtttcata ttgtctcctt attcacttgt
    26881 ttaatgtgat tggctttcca gcatttccac atagtcaatc gcgacacaga gggaaatgac
    26941 taggtctgca taagcgtctt caagaaccgt tacggtatag gtagaggtca gatggaagag
    27001 ttccttctta atttccgcaa tcaactgatc gcgatcatcc agcaatttga aattcaaatc
    27061 ccagatattg ccctcgatac gaagacctag attatcaaac tcatacttat ctcgccagaa
    27121 ggtcaacttc ttacgaatga caaaactcga gccatcccga agctgaatct caaaacgagg
    27181 aagcaaggtc aagatttctt tactgatctg actgacttgt tcaccagccg catcatagat
    27241 ggtaaaagtt ttgggaatct taaaaaatga tccctccacc tgataggcaa tttctcccct
    27301 gtcatccttg atagcgaagc gttcgcctcc aagacgaaac ttttgtttga caagaaatgt
    27361 tttcatcaac acctccaaaa atcaaaagac aagctcatat cacgaagggc gaaaaaccgc
    27421 ggtaccacct tcattcaatg aacttgtcat tctcttgttc ttatgcaatt gtatgattga
    27481 gtagcatgac ttcctagctt agatggctcg cagcaccgcc atttctctgg actaagacaa
    27541 gtgaaaatca attctcaact ttcttattat aacgtttttt taagcttgcg tcaactggaa
    27601 atgatctccg ttgaattaga ccaattccct acatctctga ttactttttc aggatatatt
    27661 ttttcttact gccatttttc tttttatccc aaattttcat attactaaac acagctacta
    27721 gaatatttcc aaatataaag gtgcctatca cccaatatat ggactcagtt gttaggtatt
    27781 gtcgatccaa gccatccttt aaatggaata gtatagcagt ttggttaaca atcataaagg
    27841 ttggccagaa actttttttg aaaaaagtag acattttcat tatttgttgc cgctttctgt
    27901 aaggttaata ctcaataaaa atcaaaaagc aaactaggaa gctagcctca agctgtactt
    27961 gagtacggca aggcaacgct gacgtggttt gaagagtata ggcttagtat actactaggc
    28021 aagcaaataa gcaaataaac aaataaacaa ctagaataga aaaagatagg gctctaaaaa
    28081 ctgacttcta ttccttaaaa acgaaccagc ttgactgatt cgtcttctta cgtttatctc
    28141 ctacttccga tacattttaa actgtaggaa gaggtcgcta tatttccctg tccatttatg
    28201 gtcaaatttc tcataaactt ctaggtgttt catggtttca acatcgggat agaaggcctt
    28261 atcttccttt gtttcctctg ggagcaattc cttcgctggt aggtttggtg ttgaatagcc
    28321 gacatactcc gcattttgga gagcattttc aggtttcaac ataaagttga taaaggcata
    28381 ggctgagttt tggtttttaa ctgttttggg aatgaccata ttgtcaaacc aaagattgct
    28441 ggcctctgtc ggtaccacat aacgtagatt ttcatttttt tctaacattt ggctggcttc
    28501 accagagaag gtcacgccga ttgcaacatt attctgaatc atatagccct tcatctcgtc
    28561 cgcaacgata gccttgatat ttggagtcag tttgtagagc ttatccactg tctcttccaa
    28621 ctgctgcaga tccttggagt tgaggctgta gccgagggaa ttgagtccta gtcccagcac
    28681 ctcacgcgcc ccatcaaaga gcatgataga attcttatac tccggcttcc aaaggtcatc
    28741 ccaatgctca ggcgcttcat ctaccatggt ttcgttgtag acaattccta aggttcccca
    28801 gaagtaaggg atggagaatt tattacctgg gtcaaaggac tggttgagaa actctggtcc
    28861 gatattttcg attcctttaa tttttgaata atcaagcgga accaagaggt cttcgtcctt
    28921 catcttgtta atcatgtatt cacttggaat ggcaatatcg taggtcgttc caccctgctt
    28981 tatcttagtg tacatggctt cgttggagtc aaaagtctcg tactgaactt gaattcctgt
    29041 ttcttctgta aactgagtca agagttcagg atcgatatag tctccccagt tatagataac
    29101 caatttttga ctatctcgac tattgatttt actatctaaa tgagtcgcaa ttccccacaa
    29161 gacaaggata atcgctgcaa ttcctgctaa aaatgaatag atttttttca tgcttgctcc
    29221 tccttctcac gagagataaa gtaataacct acaactagga taatactaaa gagaaagact
    29281 agagcagaca gggcattgat ttctaaggaa atccccttgc gagcacgaga gtaaatctcg
    29341 actgataggg ttgaaaagcc atttcctgtt acaaagaagg tcacggcaaa atcatccagc
    29401 gaataggtga aggccatgaa gtaacctgca atgatagacg gagtcaggta aggaagcatg
    29461 atttccttaa acatctgaaa ttgactagct cccaagtcat aggccgcatg aatcatgtcg
    29521 ccattcattt ccttgagtcg aggcaagacc atcaagacca cgataggaat ggagaaggcc
    29581 acgtgactag atagaacggt caaaaagcca agtgaaaact tgagttgggt aaagagaatc
    29641 aagaagctag caccaatcat aacgtcaggc gcaaccatga ggatattatt gagtgataga
    29701 aaggcttctt ggtatttctt acgagactgg tagatgtaaa tggcaccaaa agtcccgata
    29761 atggtcgcta tcaaggctga taggaaggcc aagaaaaatg tctgagccaa aatcagcatg
    29821 agtctcccat ctccaaacat ggtttcaaag tgagtccagc taaaacctgt aaagctattc
    29881 atatcatcac cagcattaaa ggcatagcca atcaagtaaa aaatcggcag gtagaggacc
    29941 agaaagacca gtcccagata aaggttggca aattttttca tcgttctctc ctttccttgg
    30001 tcacccacat ggtgatgaac atggtcagga tgagaatcac accgatggtt gaacccatac
    30061 catagttgtc attggttaga aaattctgct caatagccgt cccaaaggtg ataacgcggt
    30121 tcccaccaat caaacgggtc agcatgaaga gactcaaact tgggataaag accgactgaa
    30181 ccccacttcg cacaccgttc atagatagag ggaagatgac atgacggaag gtctcccact
    30241 tagttgcacc aaggtcgtaa ctagcattga tgagattatt atccatatcg tctaagacat
    30301 tgaaaatcgg caaaatcata aaggggagct cgatgtagct tgcgacaaag ataaaggaaa
    30361 aatcggtaaa aagcaactgt tgtgaaccaa ttccgataaa ttccaagaat tggttaatag
    30421 agccattttg accaaaaatc ccgataaaag cataggcttt aaggagcaaa ttgatccagg
    30481 taggaaggat aatcagcatg agccagagtt gacggtgctt gagacgggtc aaaaagaggg
    30541 ctgttggata gctgataagc aaggtcacaa aggtcacaat ccctgcataa agcactgagt
    30601 tgaaactcat tttgaggtag gtcaagtttt gtgacgcaaa gtaagattta taattttcta
    30661 aactaaactg gccttcaatg ttgaaaaagg attgtccgaa aatcaagacc aagggtgcca
    30721 atacaaagag ggcaatccaa agcatgtagg gcactacaaa gagtttagag cttgttttct
    30781 tcatctcttt cctcctcgat cgcattgatc agacctgctt cttgctcttc gatttccacg
    30841 tattcttcga tacgagcatc gaactcttct tcggtttcat tgagacgcat gatgtggatg
    30901 tcttctgatt caaagtccag accgatttcc tcacccacga tggccttacg ggttgagtgg
    30961 atcatccatt catttccaag ttcgtcatag gcgataattt cataatgaac tccacggaag
    31021 agctgggtgt cgaccttaac ttggagtttg ccttcttcag gaagggtaat gcgcaagtcc
    31081 tctggacgaa taacgacctc aacaggttca tttggcttca tcccaccatc aaccgcttca
    31141 aagcgtttgc cgttaaattc gaccaagtag tcctcaatca tggtacctgg caagatgttt
    31201 gactccccga taaaggtggc aacaaagtgg ttgattggct catcgtagat gtccacaggg
    31261 gttccagact ggacaatctc gccatcattc ataacgaaaa tccagtcact catggcaaga
    31321 gcttcttcct gatcgtgagt gacaaagaca aaggtaatgc ccaatcgttg ttgtaattca
    31381 cgcaattcgt actgcatgtc tgttctcaat ttcaagtcca gcgctgataa aggctcgtcc
    31441 aacaagacca cacggggttg gttgatgata gcacgggcga tggccacacg ctgacgttgt
    31501 cctccagaaa gtttgcggat ggaacgtttt tcataacctt ccaactgaac catcttgaga
    31561 acttccgcta cacgctgctc gatttctttc ttatcaattt tacgcaagcg aagtggaaag
    31621 acaacatttt caaacacatt catatgtggg aacaaggcat aggattggaa gacggtatgt
    31681 acgtcgcgct tgttggttgg aatatcattg atacgaacac cgtctagcat gatatctcct
    31741 gtcgtcgcat ccagtaaacc tgcaataatg tttaggatag ttgatttccc cgaaccagat
    31801 gcacctagaa gggtgtagaa tttcccttct tccaactcaa agttgatgtc tttgagaacc
    31861 ttggtgttgc tgtcttcaaa aactttagag acgtttttga attcgataat tggttttttc
    31921 aattggcata aattccttct ttttcataga ttaaccgatc ggggctctgt caggtcccca
    31981 ctacctcttg cagggagtaa aaccacctgc atacatcttc gctaccgata ggctttcacc
    32041 ccctttccat gacatagcag cagcttttca aatacagctt cctacttgct ttcacccaag
    32101 atccggactt ctctttcaag cgtaatacct gagtgttcct tgactttttc gataaccgat
    32161 tggatcaagt cctcgtagtc tttggccgtt ccatctgcga cattgatcat aaatcctgca
    32221 tgcttttctg acacttctac gccaccgata cgatagcctt tcaagccagc ttctgaaatt
    32281 aactgacctg caaaatgccc gactggacgc ttaaagaccg agccacaaga tgggtattcc
    32341 aaaggttgct tgagttcacg taggtgcgtc aagcggtcca tttcctgctt gataacctga
    32401 tgggttcctg gggctagggc aaatttaact gacaagacaa ctgcaccaga ctcctgaata
    32461 gctgaatggc ggtaaccaaa agccaagtct ttagcagaca gggtttcgat ttctccatcc
    32521 ttggtcaaga ccttacaaga ctgcaagatg tgagcaatct cgccaccata ggcacccgca
    32581 ttcataaaga cagcaccgcc aacgcttcct ggaataccac aagcaaactc aaagccagtc
    32641 aaactatgac ggagggcaat gcgagttgtt tcaatcaagt tagccccagc ttctgcttca
    32701 atggtatagc catcaacaga aacgttattg agcttgtcac acaagatgac aaatccacga
    32761 atcccaccat cacgaacgat gatattgctt gcattgccaa gaaccatcca agggatattt
    32821 tcttggttgg caaatttcac aaggcgcgcc aactcaaaac gatttcgtgg aaagaccaaa
    32881 taatcagcct ctccacctac ttttgtataa ctatagctat gcaagggttc cttaaaacgg
    32941 atatcaattc cttctaagat ttcaagcatt ttttctctta cagacatgtc actcttcctt
    33001 ttacaaaatt cattccatta taccattttt agagacattt gacgaccata aaaatacctt
    33061 gtttggattt tgcataagaa aaagaggttc cccccttttt atgatttttt acaaaagatt
    33121 tccttggttc cataggcgac cagaacgagc tccagtgcta gaatcacttc aaccaagact
    33181 ggatttgtca accagcctac ttggaaaaga gatggtgcca gatcaaagaa ggcatgcaag
    33241 ccataggctg ctaggagata aatccatttc ttctggcgaa cagcttggta aacccaaact
    33301 gtcaaaagta attggaaacc aagcgccaag attcgctcaa aaccaagcaa ataaatctgc
    33361 cagaccgaaa gtgactgaat ggtttttaac atattttcag acagtaattg cataacctgt
    33421 ggattctgag tttgaactgc cgaaagaacg atgtaaagat tgagtaaact aataaggcct
    33481 agaaaaatca actccaagcc accatgccct aggccatagg ccaaggcatc tgccttttcc
    33541 aaacttctct ttttctccaa ccatttgaag aaaacaagac gagccgtttc ctcaaaaaat
    33601 gctgccatgg ctaggccata gataatatag ataagcggat gatcttgcaa gagggcaata
    33661 ctaccgtctt tttgaggatg caaaatcaag atatgcacca gtttttccaa aatctgcgaa
    33721 gagacaaaga aagcgactgc acccaagccc aagacagcta gattaatctg gtatttcttt
    33781 ttggtaaacc aaatgcttcc tatcagaaaa gctagcaaca gcaacatggt aataatgata
    33841 tgaatggtca ttttcttctc ctattctgcc ttttcaatat cttttttcat ctcgtcaaca
    33901 ttgaacttag caaacaagta ttgacggtct tggactggga aacgttggtc caactggtca
    33961 actgctccca cctcgataat gccttccttg atgacaaaat ccatgacatg tccaccgaag
    34021 gtcaaatcat ctgatatgaa gtgcagatgg tagcctgcca cactgacccc atggaaaatc
    34081 tgaggcgtcc agaaaccaac aatagtcccc gcaacattgt cacgactata ttccggttga
    34141 tgggttgcga tatcagcaaa cttggtatcg ggtgttgact taggaatcat acgcacatgc
    34201 atatgtgaaa attccccccg aatcttgata gagcggaaaa gattttcccc atcataataa
    34261 gactcaattc gttctttcaa ttccttgtct gtcatctcaa agcgctggcg gaaaatgacc
    34321 tctgcctgat gcggtaccac tgcagcgtaa ggaataaggg catctggtga cacttccaca
    34381 atttctggct ggtctcctga ctctttggcc tgataagcct tgccatccaa gacaatcaat
    34441 tccccatcaa tggaatccaa ggttcccaaa ccaaggtcac catgttctag caattctcct
    34501 actgtcatag taccaccata aaggccagcc atcaaggctc caagagtatt gtattgaaat
    34561 aatttcactg gttcctgcac gttcttatcc attcactttc ttgtctgtta ttctataaat
    34621 cttactccta agtataccac atttgcccct agatgtgaac gagagaaacg ctctagacat
    34681 tgccaagaag gaaaaaaaag ggtacaatgt aacaaaatca agggaggtct ggaatgaaga
    34741 aacaaagcaa gtacaaagag gtcgtttcct atctgaaaaa tggtatcgag tctggacgat
    34801 ttccgacggg tagtcgcctg ccttctatcc gtcaactgag ccttgacttt cactgcagca
    34861 aggacaccat tcaacgagcc ctgctggaat tacggcacga acaatacctc tatgccaagc
    34921 ctcagagtgg ctactatgta ttagaacaag ggcaacatca agacctagaa atcgaggtta
    34981 ccgacgaaca tgccagtgcc tatgacgatt tccgactctg tgtcaatgaa accttgattg
    35041 gccgagaaaa ctacctcttc aactactatg acaatcaaga aggattagaa gacctaagac
    35101 aatccattca caaactcctc tttgaccaag ctctctactg caaggctaac caactagtac
    35161 tgacttctgg aacccaacaa gccttgttta tcctctctca aatatccttt cctagccaag
    35221 ccaaggaaat cttggtggaa cagccaacct accatcggat gaatcgcctc ttgattgcac
    35281 aggggctgga ctatcaaacg attgaacgag gcattgatgg gattgacttg gaggagctgg
    35341 aaggccactt caaaacagga aaaattaagt ttttctacac cattcctcga tttcactatc
    35401 ccctaggcca ttcctattct gagcaggaca aacgagctat tcttgactta gcagccaaat
    35461 atagtgttta tatcgtcgaa gacgactatc tgggggattt ggactctaaa aagggccaga
    35521 ccttccacta tctagataca gaggaacgcg tcatttatat caaatccttc tcaaccagcc
    35581 tctttccagc cttgcggata acagcactca ttcttccaaa tactatcaaa gaagccttcg
    35641 tggcctacaa aaatatccta gactacgaca gcaacctcat catgcaaaag gccctgtcac
    35701 tctatattga cagtcaattg tttgaaaaaa atcgtttggc tcgcttgacc aatcatgaat
    35761 cttaccaaaa acaaatcgag gaaaggataa ctaaaacacc ttgtcccctt cctcattatc
    35821 ccctacacga tggtttattg ctagacctga gacagtatcc taaaatcgcc agtctcaaac
    35881 acagtcaact gggcttggac ttctttgaag aggcctattt aagcacctgt ccttatcaat
    35941 ttgccaaggt gtccctagac aatctggaaa atgttttaaa ctatttaaaa gcagaattgg
    36001 aatgacatcc aactctgctt tttcttattc ttcaacttca gttaaacttg ctgctttttc
    36061 aagatagctt tggtaagttc cttcatcctt aagtttttgg atgaccttgt ctaccacttc
    36121 tttcaaatca tcactatttt ttctaagagc aacgacattg gcgtcgccgt ccttcatctt
    36181 caagctgaca gttgcgacag ctaagccagc gtttttagca gcataactaa gtgcaacagg
    36241 ctcatccata tgaacagcat ctatttttcc agcctgcaat tcattgactg cttcacccat
    36301 attagttagg gaagttaatt gagcttttgg caattgttcc ttgaccattg attctggaac
    36361 agtccctttt tgggctgcaa tattagcact ttctaggcta gttaaatcct tgtatttttc
    36421 cacatcagcc ttacgaacca agaaactaat cttgttttca tagtatggga ttgaaaaatc
    36481 aaagacttct tttctctcgt cagtagcact aattcctgca actgctaggt cagccttacc
    36541 agtttgaaga ctggtcaaaa cattgtcaaa actcatgctt aagatttcca acttaacccc
    36601 aagttcatca gcgatagcct gagccatgtc gatgtctgca ccgactacct ggttctttcc
    36661 atcaaccaat gattgaaatt caaagggtgc atagtcagga ctggttgcca caactaattt
    36721 ccccttttgc ttaatggcct caacagctga ctgagaacca ttagaacctg actggcaagc
    36781 tactaagaag aaacttgcaa gaaacttgca agaaaattac ataaaacaaa tatccatttt
    36841 tttgatttca taaataaaca acctttctgt taattttgta taattataac gatatccaag
    36901 ttacttgtca agtgtttttt aaatttttat ctcaaaaata ttttttcgtt caaaaaaagg
    36961 agccatcagt tgatttcaag ctccttttta tacagaatta aactatttta tagttcgaca
    37021 atcttacctg tttcaaagta gacaacccat tcacagatat ttttagcata atcaccgata
    37081 cgctccaagt aggaaataac ttggaaataa tcacgacccg taacaatggc ttctggattt
    37141 ttcttaatct cttcagtcgc aaggtcacgg atagtttcaa aatagtggtt aatttgctca
    37201 tccatggagg ccacccggta tgcgtcgtca acagaaccat taagataaag atcaagtgct
    37261 gcttcaacaa cgcttttaac ttcacgtccc atttttttaa tttcttcctc tacagctggg
    37321 atgcgttctt cccccttcat acggatggtt gcctgggcaa tggctacagc gtgatccccc
    37381 atacgctcca catctgatac agccttaagg acagtcaaga ctgtacgcaa atcttgagag
    37441 actggttgtt ggagtgcgat catttcaaat gatttctttt ccagtttcac ttcgtattca
    37501 tttacttctg catcatcttc tatgacctct tttgccaggt cacggtcatg cgtgacaaaa
    37561 gcacgtaccg tacgattgat ttgtgagagc acttcttgtc ccatagcgta gaactggtta
    37621 tgtaatttct ctaaatcttc ttcaaattga gatcgtaaca tctttcatct ccttatccaa
    37681 attttcctgt aatatagtct tccgtttcct tgtgttgggg atcaaggaac atctgcttgg
    37741 tatcattaaa ttcaatcaaa tctccatcta ggaaaaatcc tgtcttatca gagatacgtg
    37801 aagcttgctg catggaacgg gttaccagaa gcatggtgta cttgtctttt agaccataca
    37861 aggtttcctc aattttacca gctgaaatcg gatccaaagc cgaagttggc tcatccaaga
    37921 ggatgatttt aggactagtt gccaagacac gggccacgca gacacgctgc tgttgaccac
    37981 ctgacaatcc aatagctgaa tcatatagac gatccttgac ctcatcccag atagaggcac
    38041 cttgcaaggc tttttctacg gcttcatcca gaacctgctt atccttaatt ccattgatac
    38101 gaagcccgta gacaacattc tcatagatag tcatagggaa aggattaggt tgttggaaaa
    38161 ccattccgat ttccttacgt aattcaaccg tatctgtacg cggactgtag atgttgtgac
    38221 cattgtacac cacggatcca gttgtggtca cctctggatt gagatctccc atgcggttga
    38281 gagacttgag gagggttgac ttccctgatc cagatggacc aatcaaggct gtaatttcct
    38341 taggttggaa agatagggaa acactattca aagccttctt tttattataa taaacggaca
    38401 ggtctgatac ctgtaaaatc gcatctgtca tacggtttcc tttctaacca aagtgaccag
    38461 atacatagtc attggtggac tgtagcttgg cattttggaa aatagttgca gtcttatcat
    38521 actcaatcaa atcacccaag taaaagaagc ctgtatagtc acttgcacga gcagcctgct
    38581 gcatattatg cgttacaatg atgatggtaa agtttttctt gagctcaaac atggtctctt
    38641 ctagttgcat ggtcgcaatc ggatccaagg ctgaggctgg ctcatccatt aagaggatat
    38701 ctggcttaac agagatggca cgagcgatac agagacgttg ttgctgacca cctgataagg
    38761 tcaaggctga cttgtggaga tcgtctttaa cctgatccca gagggcagcc tgacgaaggg
    38821 aggtttctac gatttcatct aggacttgct tatccttaac tccagcacgt tcatgcgcaa
    38881 aggtgatatt acggtaaatt gacttagcaa atggattggg gcgttgaaaa accattccaa
    38941 tgtgtttacg catttcataa acgttgattt ctggacggtt gacatcaatt ccacgataga
    39001 gaatctgccc agttacttta gcaatatcaa tagtatcatt catgcgattg agactgcgta
    39061 agtaggtaga tttccccgac cccgacggac caatcaaagc tgtaatttta tttctttcaa
    39121 attgcatatc aatcccctta atggattcat ttttaccata gtaaacatgg acatccttag
    39181 tagaaagggc tactttttct tcaggaaagg taaggatatg cttctcatcc cagttatatg
    39241 ttgacatggc ttctccttta ggcagcggtt aatttcttgt gtagatagct tccgaactta
    39301 cgagctccaa agttaaaaat caggataaag atcaggagca cagcggcaga acctgctgat
    39361 acaatggttc catctggaat agtgccttca ctattgactt tccagatatg gacagccaag
    39421 gtttctgctt gacggaagat agagatgggg ctagtcacac tgaggatatt ccagttagac
    39481 cagtcaagag ctggcgccga ttgccctgct gtatagatca gagctgcagc ttcgccaaag
    39541 atacgaccag atgccaagac gacacccgtt acaatacctg gaagcgcttc tggaataaca
    39601 acatgaacca ctgtctccca gcgagaaatc ccaagagcca gaccagcctc acgttgggta
    39661 tggtgaacgt gtttcaaact atcctctaca ttacgcgtca tctgaggcaa attaaagact
    39721 gtcaaggcca aggcacctga aatgattgaa aatccatact caaactggac tacaaagatc
    39781 aagtaaccaa agagacccac caccactgat ggtaaagagg acaagatttc aatacaagtc
    39841 cgcacaaagt tggtaacagg acctttttta gcatattcag ccaagtaaat cccagctccc
    39901 atagaaagag gtacagaaat aatcaaggta atgaccaata ggaaaaagga attgtaaagc
    39961 tgaatgccaa tcccaccacc tgcttgaaaa gcagaagacc ttccagtcaa gaaagaccaa
    40021 gagatatggg gcaagccccg aaccaagata tagagaatca aggaagccaa gattgtcaca
    40081 atgatgctag caatcgtata gaggacagct gttgcaagtt tatctaattt cttagcgcgc
    40141 ataatttttc tttcctcttt ctttcgtaat caatttaatc acactgttaa aaactaaact
    40201 catcaagagc agtaccaagg ccagtgacca gagaacatta ttatttacag ttcccatgac
    40261 agtgttccca attcccatag ttaatataga agttaaagtt gcagctggtg tggtcaagga
    40321 agttgggata acagctgagt ttccgacaac catctggata gctaaagcct caccaaaggc
    40381 acgcgccatc ccaaagacca ctgcagtgaa aataccagaa cgggccgcct tcaagatcac
    40441 acgccagata gtctgccagc gagtggctcc catagcgaaa cttgcctcac gataataacg
    40501 aggaaccgca cgcaagctat ccgttgtcat aaaggttacg gtcggcaaaa tcatgacaaa
    40561 gaggacggaa atccctgaca aaatcccaaa accagtccca ccaaagacac tgcgaacaaa
    40621 gggaacgacg acttgcaagc caataaatcc gtacactact gaaggaatcc caaccaggag
    40681 ttcaatagct ggttgcaaaa tcttcgcccc ttttggtgat acttcggtca taaaaactgc
    40741 tgcaccaata gcaaagggtg ttgcgataag ggctgagaga atggtaacga taaaggaacc
    40801 caaaatcata ggaagggcac caaattcttt actagaagga ttccaagttc ctcccaaaag
    40861 aaagtcaaag atattcacac cattgacaaa gaaggtcgac aagccttttt gcgctacgaa
    40921 aaccaaaatc atggccacaa ggatgactat caaagaaaga caggcaaagg tcaaaccttt
    40981 tcctaatttc tccagacgag aattctttga tggaagcaac attttcttag ctaattcttc
    41041 ttgattcatt attgtctccc ttccaacact gtcacagttc cggcagcatc tttttcaacc
    41101 ttcatttcct taatcggaat atacttcaat cctttgacaa tcccttcttg ggtctcatcc
    41161 gagagaacaa aattgagaaa ttctgcagct aactcattgg gctgccccaa tgtatacata
    41221 tgctcataag accacaaggg ccaattattg ctacttatat tttctggact taagtcatag
    41281 ccattcaact tcatgctttt gaccgaatca tctatatagg taagagataa ataagagata
    41341 gctcctggac tttttgatac gattgatttt accgctccat ttgaatcctg ctcctgactt
    41401 tgcatggcag actgaccttc cataatgaca gtatcaaagg tagcacgaga gccagagccg
    41461 gctgcccgat tgataacaga gatgggtaag tccttaccac caacctcttt ccaattggtt
    41521 acctcaccta tgaagatttg acgaagttgc tctgtcgtta gattatcaac atcaacctcc
    41581 ttattgacaa tcagagccaa gccagctacc gcgaccttgt ggtcaacaag agcagaagca
    41641 tcaattccgt ctttttcctc agcaaataca tctgagtttc ctatatcaac tgccccagac
    41701 tgaacctggg acaagcctgt accagaacct cccccttgga cattgaccgt ttttccaaca
    41761 tggatcgtgc caaattcatc tgccgctact tcaaccaagg gttgcaaggc agttgagcca
    41821 acagccgtta tggattctcc acgatcaatc cagctagcac agcctactaa acaagccgtc
    41881 agccaaaaag cgataagaga cagagcaagc ttttttcttt ttttcactgt ttttctcctc
    41941 gaaaataatt atgaatactg tgaatttttt aagtagttct ttatgagttg acgcatgaat
    42001 tcttaccaaa tttctgcgca attgattatt tatataatat aggctatatt actctttcct
    42061 aacctccttt tttcatatgt ggataaaatc tcttgtctat cccttccccc attgtcaccc
    42121 attatagtca tttcgtgtct ctttttcccc tttttaatgc aagggaaatt actctcctta
    42181 gatgataatc taaaagctag aaaggtatct caaatctctc tactctccca gactagttta
    42241 caactaaaag gaaaagattc tattttatga gaaatctagt ttacaagcgg taagaacgct
    42301 aataactaaa cttcttgtac tctttgaaaa tctcttcaaa gcagtgtttt gagctatcta
    42361 tggctagctt cctagtttgc tctttgattt tcatcgagta gtaaaactac atgtgatggc
    42421 aatcaagata tcaagaatca tcctactaaa aaaatccata ctttcactat aacatagaat
    42481 aagatatttg actagcattt tcatttgaat ctgaggcctt ttggaaaata atttttcaaa
    42541 acatttccag taacctttgc aaagcccaag ccattgcctt taaccaaaac ttggtaccaa
    42601 ccatttggca gactttctgc cagctgaacg gtttctccag ccgcatactt gacaaactct
    42661 tcttggccaa tttcaaccga ctgttcgacc tgactcggtt tcaaggctaa accaagagcg
    42721 aaactgggct caaagcgttt cttcttaaaa gtacccagat gcagtccatt gcgagcaatc
    42781 ttgagcttcc ctaaatctgg caaaagttct ggcaagagat aaagctggtc tccaaaaatc
    42841 tgcaagatac ccggtagatt gaccttcaaa tggttttggg caaattcctg ccacaaggca
    42901 acttgttcac ggctgaggtt actcttactt gccttaaatt taggagctgg attgttaccc
    42961 ttaaactgta gatgggcaac aaactgaccc tctcccttaa actgatgagg gtacatccga
    43021 gccgtttctg gcaggtcaat accagctacc attccattga tatgctctac tggcaacaag
    43081 tcaaaatcat actcttccag caaccaattg acaatctctt cgttttcctc gggtgcccag
    43141 gtacaggtcg aataaaccag acgaccacct tcagctaaca tggtcactgc atcctccaga
    43201 atttctcttt gcaagctagc acattgactc ggataatcta agctccaata gtccatagca
    43261 tcaggttgct tacgaaacat tccttcacca gagcaagggg catcaagaac gattaagtca
    43321 aaatagcctt taaagacctt gaccaagcgg tcggcagatt cattggtcac cacgacattt
    43381 gtcgctccaa aacgctccat gttttcaacc aaaatcttag cccgtttgct tgaaatttca
    43441 ttggaaacaa gtagcccctc ccctgctaga taggctgcca gttgagttga tttgcccccc
    43501 ggtgcagcag ccaagtccaa gaccttcata ccaggactgg gttgggctac ttgagccacc
    43561 atttgagcag caggttcttg cgaataaact aaacctgtag catgctcagg cgatttccct
    43621 gaaaccttcc catagtggcc ccaaggggtt tgagtaatgg catcagaaaa ggaaagttgc
    43681 tcttctttta agggattgac ccgaaaggcc gaaaccgctt cctcctcaaa agaggcaaga
    43741 aaatctcttg cctcatctcc tagtatttct ttatattttt caacaaatcc ttctggaaat
    43801 ttcatttaag ttcttttcct ttcgtaaata taggactgaa tttcctcctg catctcaaga
    43861 ggcaccatca tgaccggctg tctggtttga aaatcaggag cttcaccaaa aagggtcaca
    43921 acccgatagc ccagactttc ccctaaaata ctagctgcgg cataatccca tggttgcaga
    43981 taggtgagat aggtcaacaa acgccctgac aaaatcttgg caaaactaat ggccgcactt
    44041 ccatagacac gaacaccaag aaccgctcgg ctcaaatcag ccagccccca ttcattggtt
    44101 tccagcatac cactattccc tgcaatgaga aaatctccaa gtggtttagt tttaaaagaa
    44161 gctaggggcc tatcatttag acaaactgga aattccccac caccgtggta acaatcccct
    44221 ttgaccacat cataaatcag accaaactgt ccctgaccat tttcaaaata agccatcata
    44281 acagcaaaat cttcctgctg ggctacaaaa ttattggtac catcaatggg atcaatgacc
    44341 caaaccttgc cctcttgaac cgaggctcgc agacaacctt cttcagcaca aatcttatcc
    44401 tcaggataac gggacaaaat ctcaccaacc aagagttcct gaacttcttt gtccagtctg
    44461 gtcaccaaat ctgttggaga ggacttggtt tcaacacgca agtcttcctg catatggtca
    44521 agaatgtact gacctgcttt cttaacaagc tctttagcaa attcaaattt actttccaag
    44581 agaaatcttt ccttcccctt tttctttggc agcctgaact gctcggtaaa gtgaatagcc
    44641 actaaccgtt tcaaattctc gtcccaaacg tttctcttcg ctcttgcttg gcacgaccgc
    44701 tttgaatccc ttataggagt ccagtaactt tttagcctct actttgcctt cataggcagc
    44761 ttcaacatca ttaaaaaaag aaagcactga agcaagttct tcagtgctcc acgacaaatc
    44821 tagtgggtaa ctatactgtt tgttcattaa ctaataccag ctctcattct tgcttctttt
    44881 agttcttgct tacgataact acgagggaga aaagcacgaa tctcatcttc attaaaaccg
    44941 atttgcatac gcttggcatc aataataatt ggacgacgca aaagactagg atactgctca
    45001 atcaaatgaa gcaattccga taccgaaata ctctctacat caatattcaa tttttgaaaa
    45061 atttttgaac gagttgaaat gatgtcatca gtaccatttt cggtcaagga aaggatttgt
    45121 tgcaattctt ttcttgttaa aggactggtc ataatattgt gttccacaaa gggaacctta
    45181 tgtttttcta accaggcctt agccttacga catgatgtac agctcggtga tagaaatagt
    45241 gtaatcatgc ttttctcttc ttatctatac tttgctactt ctattataca aaaaaataag
    45301 gcgcttgact agggattttt agaaaaaaaa gcctattttt caagaaaaat aggctgtttg
    45361 cgaacgattg acacaattgg atttggttaa ttcactctta acgatggttt taaacgatat
    45421 atatttttat atatgcaaat taaaaacatc tttcatttca tttcctacga cttttcagac
    45481 acagatagcc aaagaagctt tcatagaggg caaaaaagag gaggaaggca tgaagaaaga
    45541 aggtctctgg caaaatcata ataacaggat ccttggctgg atcaaaaagc caggtatcat
    45601 ctcccacaaa gagaatttga tggaaaagag taaagaattg gtcaaaacca atcaaaactc
    45661 ccccaagtcc aatcatcaca ggtaagacta ctagagtcag gagacttttt cgataaagag
    45721 acaaaaagtc ctttttcaca attctattga caaagacata gaaacttggc agtgtcacta
    45781 gagctactag ctgaaccaaa tgaaagagat tcttgaccac tgcgaaatgg tgcagaccag
    45841 ctgctgacga acgaaaatca ggcatctgta agacctgact aaaaggattg gtcagataat
    45901 tcatcaagat atgaaaattg tattgaatgg tttctggttt tagatagact cgattcgtta
    45961 agtttagcca ctgaatctcc ataggataga aaatccaagc cagataaatg ggcagaagga
    46021 ttgagaggga gaggagaaag agcatagagc cccaaaagat caatttagtt ttcatcaaaa
    46081 tcccactccg caaggctaga aaccacatgt gtcggtgcga ttggcaggcc agctacttct
    46141 tctgccttag taaaacctgt cgtcaccaag agcgttggaa tgccattgtc aatcccagcc
    46201 cggatatcag tcaaataatt gtccccaacc ataattaact cttcacgttc caaacctaag
    46261 tgctcaaccg ccttgtccat aatgatggca ttgggttttc cgatataaac cggcttcact
    46321 cgtgtcgcta cttcaagcag cgtaatcagg gagccagcac ctggcaaaag accacgttcc
    46381 gtcgggatgt tgaggtcagg attggttccg ataaaatggg cacccttttg aatagcaaga
    46441 gttgctgtgg caaatttttc atagtcgact tgccaatcca gaccaactac cacgtaggca
    46501 gggttttcct tgtcttccac ataaccagcc gccttgatgg cttccttgag tcctgcttct
    46561 ccgacgacat agacggtctt ttcaagcccc aaatcattca tatagtcgat ggttgccaaa
    46621 gtcgctgtgt agacagtcga taggggcgta tcgatattaa aattctgagc caacatctcc
    46681 ttaacactct ctggagtgcg agttgtattg ttggttacaa agagataggg aatgtcccgc
    46741 ttttgcaatt catgaacaaa agtctctcca gcagggattc ggtctttccc cttataaatg
    46801 gttccgtcta aatcaattaa atagccttta tatttcatct atttctccct aagccttttt
    46861 tatttctttc caagtaatga ttgcttgggc attgataacc ccatcacttg taatttcatg
    46921 cttgctttcc agtccagtcc gttcaacagc cgatgtaatc accccacctg gtcgaacttc
    46981 cttgacatac ttgaggttga ttttcttggg aatatagtgg gtcaaaaaat ccgctcccat
    47041 gacctcaaaa atccagtcca agtatttact gttattgaca tgaccattca tatccaagtc
    47101 gtaaaaacga acatggtaat ccttgctgat cggctcttcc aaggactcat acttcggtcc
    47161 acggataagt tttttatcaa aatcagactg gtaaggagcc acaatctcag gttcaacagc
    47221 atggactttt cgactgtcgc ggtccatgag aacaaaggtc gccatcatat ggatgagctc
    47281 ctgccccgct tcattataaa tagtaaagcg acggtagcaa aaaagtcgat tgtagctcaa
    47341 ggcttccgtt tcgatggtaa tttcttccgc aaaacgaggc aaacgaacca cctcaatatc
    47401 atattctgcg ataatccaga ccagattata gtcttccaaa atggccttat cactaactcc
    47461 cagttcaatc gactgcatcc ctgaaacttg cagtgacagc aaaatcacat ctggaagttt
    47521 gatatgaccg ttcatatcag ccatatcaaa aggaattttc attttcattt gataagttaa
    47581 gcccatgatc ctactccaaa ataaatcgtt ctgctacagt atctcccaaa aagagacctc
    47641 tctttgtcat gcgaacgtgg tcaccctcaa tctgcatgag gccttgttga accaaatctc
    47701 tgacaatttc tccataaagt ccagcaaaag actgtccaaa tttttcctca aatcgcgcca
    47761 tggaaacccc ggatttcttg cggagtccca agaacatttc ttcttccatt tgctcctttt
    47821 gactcaggtg atcttctgta atacaagcat tgccttcctc aaccgcactg agataatgac
    47881 gaatgggacc atgattttta taacgtactc cattgacata accagatgcc cctgcaccaa
    47941 taccatagta ttcagcattg tcccagtaca tgagattgtg acgactttca aagccgggtt
    48001 tggagaaatt agaaatctca taatgctcaa aacctgctcg ctccagctct gcaatgatgt
    48061 actcaaacat ctccgcttct agttcctcct taggcagagg caatttccca cgtcgcatcc
    48121 ggttcataaa gaccgtatgg ttttctaaaa tcaaactata caaactcatg tggggaatat
    48181 ccagtccaat ggctttagcc acattttcct ttacttgctc catggtctga ccaggcagag
    48241 cataaatcaa atcaatggag atattgtcaa aaccagccag tttcaggcga tcgatatttt
    48301 cataaatatc cttctccaaa tgactgcgcc caattttttt caacatctta tcatcaaagg
    48361 tctgaacacc tagcgaaaca cgattgacag ccgaattttt caaaacagct atcttatccg
    48421 catccaaatc gcctggattg gcttcaatgg tcaactcttc caagacagac aagttcaagt
    48481 ttttagtcaa gccattcagt aacacctcca gttgcggagc cgacagggct gtcggtgttc
    48541 caccaccgat ataaagggtt gacaactttt caatatcata agaacgaaac tcttccagca
    48601 gatgctctaa atagctgtcg actggctgat ttttgatgaa gacctttgaa aaatcacaat
    48661 aataacaaat ctgggtacaa aatgggatgt gcacataggc tgacgttggt tttttctgca
    48721 tagtaattat tataccacaa agactagatt ccagataaaa atcaccatcc ccagatacat
    48781 agtccgtccg gagatggtga tggtttattc ttctgttata tcaatcacaa tctcttctga
    48841 gtcatcaaga gcttcggctt tttcttgcca ttgctccttg agattattta attgattttt
    48901 tgatgcttct gtcgcttgaa aagcatagga tttagcttga gcaagtatac tgtccacagt
    48961 gatttcacct gactcaacct gttcttttgt tttcagaaca aaatctgtag cctgctcctt
    49021 aacttctgtc agtttttcac agacttgctc cttggcatac tccggatctt ctctcaaatc
    49081 atctagaaaa tcttgagcct gactgcaaac ttgtttgccc ttatcacttg ttaaaaacaa
    49141 ggcaagagct gcacctgaaa ccgttcctaa aaggattgag gataatttac ccataaggat
    49201 tctccttttt tattttttga aaaatttact tgcaagacga agagctgaca gacttgcacc
    49261 agtcttgagt gtttttgaac cagctgatga agctttcttg ctcaagacac gcgcatggtc
    49321 attgaggtct gaaacagata gagataaatc tgcaacagca ctgaagagtg gatcaatcgt
    49381 agccaccttg acattgatat catctgccaa gacattgacc ttagccaaca actcattggt
    49441 atgatgcaag gtcacatcca catctgaagt caaggtttta atcgtctttt ctgtttcatc
    49501 gatgacacga ccaagctttt gtacagtaat gatcagatag accaaaaaga caatcaaagc
    49561 tagggcaaca agaatatatg caacttctaa catttagttt tcctcctctg taatatagta
    49621 aggggccttc tttcgatttt gataaataac gatcattata ccgagaccga taaggacaac
    49681 tgacagccat tgggacactc gaaggccgaa gaacatgaga ctatctgttc gcataccttc
    49741 gatgaccata cgaccgaaac cataccaaat caagtaaaag gccgtgatat gacctcgtct
    49801 gagactcttc catttccgtc taaaaatcag aatcaaggca aagccaagca gattccatag
    49861 agactcataa aggaaagtcg gttgacggta gctcccctca atatacatct ggtcacggat
    49921 aaagccaggt agataatcaa gattatccac tgttgcacca taagcttctt ggttaaagaa
    49981 attaccccaa cgccccaaac tttgagcaat cataacgcta ggcgccgcaa tatctagaaa
    50041 atcccaagta ttgatgagtt tacggtcagc aaagatatag agcacaagag ccccagttat
    50101 caaaccaccg taaatggcca aaccaccatt ccaaatggca aaaatctctc ctaaattctg
    50161 actatagtaa tcaaatcgga aaataacata gtagagacga gctcctaaaa tagccaaggg
    50221 aaaggctact aagataaaat ctaaaatatc gtctggtatg atcttctttc taggtgcttc
    50281 tttcatggtc aaataaaccg caagaatcaa gcctgtcaca atacataagg cataccaacg
    50341 aatggctagg ggtcctagtt gaatagcaat tggatcaagc attttgcacc tcatttcgag
    50401 cgattagact tgtcagtcgt tcgtcgaaca aacgggtcgc atcaaagccc atttccttgg
    50461 cacgataatt catggcagct gcctcaatca caacagagat attacgacct gttttaactg
    50521 gaatacgaat acgaggaatg gctacgccag aaacttcaag ttcctctgca ttgtttccaa
    50581 gacgatcaaa ggtcttatgt gtatcgtaat tttccaaata gacagcaagc tgaacctgtg
    50641 aagaatcctt gacagcactc gcaccgtaga gactcataac atcgataata ccaaccccac
    50701 gaatttcaat caagtgtttc aaaatttcag ctggttcacc ccagagagta atctcatcct
    50761 tggcaaagat atcgacacgg tcatcggcta ccaaacggtg accacgtttg acaagctcaa
    50821 gacctgtctc gctcttacca attccactat ctccctgaat caagacgccc atcccataaa
    50881 tatccatcaa gacaccgtgc acactggtac gttctgccaa acgagaatcc agatagctag
    50941 ataactctcc agataaacga ctggtagctg cacggctggt taaaatagca atcttacatt
    51001 ctctagcagc ctttaacatc tcctctggaa ccaccaaacc acgggcaaca atgactgctg
    51061 gtgtctcagg tagaaacatt tttttcaaaa cttcataacg gctgttggaa ggcatgctga
    51121 tcagataaga ccactccttc atccccaaaa gttgaatccg ctctggtgta tagtagtcaa
    51181 aatagcctgt catttcaaga ccaggtcgcg taatatccgc tatattgatt tccttttcaa
    51241 gcaattctgg ttcaccatag acaatgtcta gtctaagctt ttcaatcact tcttttacta
    51301 aaaccgacat catttcctcc ttcaaatgag ttcttctctc tattatatca aattgtagga
    51361 ggaagtttca gatttttgca ggctttggag tatttattta aaagaaaaag gactagattt
    51421 ctctagcctt tcattccaat tagaattttt tacgtttacg agctacttct gatttacgtt
    51481 tacgttttac agaaggtttt tcatagaatt cacgtttgcg tgtttcttga agagtaccag
    51541 ctttagtaac cgcacgtttg aaacggcgaa gtgcgtcgtc aagagattca ttcttacgta
    51601 ctactgtttt agacattttt ttcactccct tcaagttcaa tatctatttc attctacacg
    51661 taaaaggaat aattgtcaag caaaaatgcg gtttaatttt gattttttct tccatttata
    51721 caagagttga aagcgttcaa tagaacattg cttttttatt tttcgagtaa gctaagcgct
    51781 tcagcatctg cgatgatggt tacatcaggg tgattttgga ggctacttgc aggtaggttc
    51841 tcagtcactg ggccagatac tgttccggca atggcttctg ctttcgactc accgtaagca
    51901 aaaagaataa tagacttggc atccaaaatg tttttaatcc ccattgaaat agcttgggtt
    51961 gggacgtctt caatcttggc aaagaagcgt gcattggctt caatagtaga ctggtcaagt
    52021 tctactagat gcgtttgact gtcaaatgga gtaccaggct cattaaagcc gatatgccca
    52081 ttgcgaccaa ttcccaagat ttggaggtca actggatggt cagccaaaat ttggttgtag
    52141 cgttcaactt cagcttcagc attgtcctta acaccacgag gcaagaaact ttctttaaat
    52201 ggtttttggt tgaacaagtt ttcttgcatg aagtaacgat aagactgtgg attgtcccca
    52261 tcaaggccca catactcatc aaggttgaca ctggttagat ttgaaaaatc aaggtcactc
    52321 tcaacaattt ccttgtaaaa ttcaagtggg ctacttcctg tcgcaagtcc tagagtttga
    52381 gctccattag ccaatttttc cttcaaaatc tcaaaagcaa cttttccacc ttgaacttgg
    52441 ttttcaactt taataacttt catcttttca tcctccattt ctgtctatat ttattatatg
    52501 gtatagacca atttttgtca agtgaaaacg atttaatttc taaaaaaaga gcgtccaaac
    52561 ttgaaacgtg ttataatgga taggattata acttagaaag aatgaacaaa tatatgaata
    52621 cagctgattt tgatttccac ttgccagagg aattgattgc ccaaacgccc cttgaaaaac
    52681 gtgatgcctc caaactcctc atcgtcaacc gtgagacagg agaaatgcaa gataaacatt
    52741 tccactctat tattgatatg ctggaacctg gtgatgccct tgtcatgaac gacacccgag
    52801 ttctccctgc ccgcctctat ggtcaaaaaa tggagacagg aggtcatgtg gaacttctcc
    52861 tccttaagaa cactagtgga gacgagtggg aagttctggc taaacctgcc aaacgcctca
    52921 aggtcggtac tcgtatcagc tttggtgatg gccgcctcag cgctgtcgtt acagaagaat
    52981 tgacccacgg gggacgcatt gtccgctttg aataccaagg aattttccta gaagtcttgg
    53041 aaagtctggg agaaatgcct ctgccacctt atatccacga aaaattagat gaccgtgaac
    53101 gttatcaaac cgtctacgcc aaggaaagcg gctctgctgc agcaccgact gctggtcttc
    53161 acttcaccaa agaactgctg gcagaaatcc aagctaaggg tgttcatcta gtctatctga
    53221 ctctccatgt cggactcgga acctttagac ctgtttctgt ggataatctg gacgaacacg
    53281 aaatgcactc agagttctat caactttctg aggaagctgc tgccaccctt cgctctgtca
    53341 aagaaaatgg tggtcgcgtc atcgctgtcg gaaccacttc tatccgcacc ttggaaacta
    53401 ttggttccaa gtttgatggg caaatccaag cagattctgg ttggaccaat atctttatca
    53461 aacctgggta tgagtggaag gtcgtggatg ccttctcaac caacttccat ctgccaaaat
    53521 caactctggt catgttggtt tctgcctttg caggccgtga attagtctta gatgcctacc
    53581 accatgccat ccaagaacac taccgcttct tcagttttgg tgacgccatg tttatttatt
    53641 gagaaagaat ttctctaatt cttctaatac caataaatcg ctaagatatt atttcaaaga
    53701 acatctacaa ttgaaactct agctagctgt agaagacgcc tagtacattg aaattaaaat
    53761 gcttccccct agcttcgaaa atattgccat agattgcgtt gactctccaa attgattcat
    53821 ctatatttta tttcagcttc ctatattttc ttcgctgttt gtaaatcaaa atgcaagaca
    53881 catgagtagc accatatttg ttactcttat ctgtcctctc aagagactat tatgagttat
    53941 ttcagaatca ttcactactt tgaccctgac tctccttagt ctcaaaatca aagacttata
    54001 ctcttcaaaa atctcttcaa accgcgtcaa cgtcaccttg gattatatat gtgactgact
    54061 tcgtcagttc tatctacaac ctcaaagcag tactttgagc aacctgcgac tagttttcta
    54121 gtttgctctt tgattttcat tgagtattaa acaaaaagtg aacaaatctg aattctaatg
    54181 tacagaagac taggcttgtt cactttttta tagtcgctat aagatgacct tatctatagc
    54241 tttttatata taattatata ttcagacata ctattatcaa ttttgtcgca gggaggaatc
    54301 tgttaacgca cccattcacc attatcattg actctatagc catctatact tgtattgacc
    54361 gctaactcac ccgatgcatt tacataatac catttaccac caacttggaa ccattgattg
    54421 actttcatag aaccgttgct gttgaggtag taccatgaac cattaacttg tacccaacct
    54481 gttgccatgg aaccatcagt attataaaaa taccacatac cattttcttg tttccagtct
    54541 gttgttggag caactgcttt agctggttct actgctacat ctgttccttg gttagatgta
    54601 acagatacag gatacgaagg aatagatgat tgctcaggaa caacaacttt ttcaggttct
    54661 ctcgtccctc tccttatacg tctttttacc atctctttag taatttgacg agaagtagtt
    54721 tcttcaattg ttccatcacg ttcatctaca gtatagattg tagtaagagt aatttaccaa
    54781 tttctcctac ttcttctact tcttgacttt tatcaagagt tgggccatcg agatattctg
    54841 tttcgattgg aatttcttgg acaagaactt ggggcttggt tcttttttta acaactcttg
    54901 tttgagagtc tttttttgac ttaaagtact ctcagttact tgtccactct ttccatctac
    54961 attataagtt atcgttgtaa ctgttttccc attctttcct agagtaatct cttgctcctg
    55021 tcctgcagaa aggtcattgt ctgcttcata tttagtagca aatggaacaa gaacttcttc
    55081 aaccttgctt ttagctggaa ctttgataac tgtatccgtg gcttcttttc tatcaacagt
    55141 aacctgttcg gtaacataac cagtctctgg attaacatcg taggtccttg tcgtagttac
    55201 atagccatcc tctccatcaa ttgtaacagg attttcacta cggtcttttg tttcatcttt
    55261 ttcataacga attcgcgtac ttgaaatttt cttggttact accttaggtt tagtcgctac
    55321 ttttacaata atatccccat tgtcagcgtc atcatactct attccctctt ctttatctct
    55381 agtatcatct ctgacatatt gaatcccatc agcagcatga acaaaacttg tattcagatt
    55441 cctcctaaaa ataaagttag cccgattacc gcagaaccaa aaatctttcc gagtttacgt
    55501 attgcatagc gcttattagt attagatttt gccattacat cctacttcta gtatagcatc
    55561 ttttctatca aacgttaaac aatatacttt atatataaaa tagacttaga atgatatatt
    55621 gattattgaa ctaacacttt aactatatcg taatcaatct catatataaa ggattgcaga
    55681 catcttatct aaatacatgc taatatattt agatacaaac attccaactt gataattttc
    55741 actcatcttt catcattcct tatacaacta tgcagtataa atagaatagt tttctcatca
    55801 gaatgagact attttaatat tagataattt gatagaaaaa acatgaataa aattggttga
    55861 atttaattcc ctaactcgat agaggattct attttaaatt taacaacagc taggattaca
    55921 ctataaaaag tacattacca cctgttcggc tggagaatct agtcgattag ctccctctta
    55981 gttacaagct tcaagcgatt ttttaactaa cactaaacca gattaaagaa ggaactagaa
    56041 catctgtctt gtactaatca atatgctatt ttaaagtttc aaataaactt ggaatgctaa
    56101 ttattatatt tacaataaat agttgacttc gatagaaaca gaaaatagaa ttcaacattc
    56161 ttccctactt ataccataat aaataagatc cgcaagatag ccttttctct ctacaccatt
    56221 aacaatggtt tgtagatagg acattccagc cttttccatg acacgacctg aagctgggtt
    56281 gagactggca taacgtgctc tgaccttttg aaaacctgct tgagtaaaac aaaagtccaa
    56341 gatagctttc aaagtctctg tcatcatacc atttccccag taagccttgc ctaacacata
    56401 gccaatttca cagcttaaat cagcctcgtc tatcttaaca atgctgatat ctcctattac
    56461 ttgctctggg ttttctttta gacaaatggc ccatttataa tagttgagat tagtatagga
    56521 agcaacccaa ttgcaaatcg agtttcgagt gatttcgaca tcaggatggg gatcccaggt
    56581 aacataggtc agattctcag cggatgaagc ccaattttga aacatggctt ctgcatcact
    56641 ctccacaaat cttcttaaaa tcaaacgttc tgtctgtaat atttgcgtac cgatagattt
    56701 catgatgcta cctcacttcc ttatttctat actcgtagta aaaaggctca actcagtaac
    56761 agctagaaaa cgagtttgtc ttctaatttc ttgacgcagt tgctgcctgg tttgaaatgc
    56821 gctctagcaa gctttttcaa acctagtcat cgttgcaggg gtaagacgat gaaatcgaac
    56881 tcttcgagtt actgaatctg cctcattttc ttatttctaa actcggggtt caagatcccc
    56941 cagaccttct ctcactttct tatttcaaga tgagaggctg aaaacctcca gtggaggttt
    57001 ttcttgcttt ttaatttcag ggcaagaagc taaaaaggtc ccccggacct ttttactccc
    57061 aaaagtcatc aaatacggtg atgggtaagt ggcgtttgtg ttggcctttg tgccaccagt
    57121 tttcaatggt cgcttgagct tctgggctga ttgttttgcc ttctaggtag tcgtcaatct
    57181 ctgcgtaggt gactccaagt gcgacttcgt cagctaggcc tggtttatct tcttctaggt
    57241 ctgccgttgg gattttttca taaagggctg gctctgcgcc aagtttctgc aagagctgtt
    57301 ttccttggcg tttattgagg cggtaaagag ggagaatatc cgcaccgccg tcaccaaact
    57361 tggtaaagaa acctgtgata ttttccgcgg cgtggtctgt tccaatgacc gctccgctat
    57421 gggaaccagc aagggcatac tgagcaatca tacggcaacg tgccttgata ttccccttgt
    57481 tgaagtctga aacaggacta cctgtcgctt caactgcagc tgtcatggca tcagctgatt
    57541 ccttgatatt cacaaccaag ctgacatctg gctggatgaa ggctagggct ttttgagcat
    57601 ctgcttcatc agcttgcact ccgtatggca ggcggacagc gataaatttg tagctatcgt
    57661 ctcccgtttc agctcgcagt tcttccatag ctaattgcgc caaacgtcct gccaaggttg
    57721 agtcttgtcc cccagaaatc cctagtacaa aggtttttag gaagggatgt tttttcagat
    57781 atctttttaa gaaatcaata gaacgacgga tttcttcctg ggcatcaatc actggtttga
    57841 cacccagctc ttggataatc gtttcttgca aactcattct tcttctcctt caccaagggc
    57901 ttccttgcgc atcttatcaa tcaagtccat cttatcttgc catacatcac gcgccaaatc
    57961 cactggatag tgctgcggat tgagcacacg cttatactca tcccacaact tgtcaaattc
    58021 cttacgggca taatcctgaa tgtcagtcaa actaggcaag ttgtaaacta atattccttc
    58081 tttgaagata tccaccaaga gaggaacggc atcaaaatta cgaaccgtct tcttgatgta
    58141 tgtataggtc ggatggaaca tcttgatttc tgtcatgtcg ctaatatcca caccatcata
    58201 agtgatatag tcgccttctg acttgccttt ttcacgactg gtaatgcgcc acacctgctt
    58261 cttacctggc gtagaaactt tttcagcatt attagacagc ttaatcgtat tgcgcatctg
    58321 accagtttca tcttcgattg caacaatctt gtaaaccgcc ccaagagccg gctggtcata
    58381 ggctgtaatc agcttggtac ccacacccca gacatcaatc ttggcctttt gcatcttgag
    58441 gttaaggatg gtattttcat ctagatcatt agaagcataa atcttagcct ctgtaaatcc
    58501 agcctcatcc agttgctgac ggactttctt agaaatgtag gcaatatccc cagagtcaat
    58561 ccgcacaccc ataaagttaa tctgatcacc cagctcacgc gccacctgaa tggcagctgg
    58621 tacaccgatg cgaagggtgt cataggtatc cacaagaaag acacaatttt tgtgggtcgc
    58681 agcgtaagcc ttgaaagctt catagtcatt gccataaacc tgtaccaagg catgggcatg
    58741 ggttcccaaa acaggaatgt caaagagctt acccgcacgc acgttgctag ttccattggc
    58801 gccaccaatc acagctgcgc gtgttcccca gatggccgca tccatttctt gagcccgacg
    58861 tgtcccaaac tccatcaagg gttcatcttc gataaccgaa cgaatacgag ctgccttcgt
    58921 cgccaccaag gtctggtagt tgacgatgtt caaaagagcc gtttcgacca actgacattg
    58981 ggctagaggt ccttccacct gcacaatcgg ttcattagca aaaaccaaat ccccttcttg
    59041 ggcagaacga acggtcaact ccaacttgaa attgcgaagg taatccaaga acgccccatg
    59101 ataaccaagc gactccaaat aggctatatc actatctgaa aaacgcaagt cttcaagata
    59161 gttcacaatt ctttccaaac ctgcaaaaac cgcatagccg ttcttaaaag gctgttggcg
    59221 gaaatacacc tcaaagaccg ccttcttatt gtgaatccct tggtcaaagt aaacctgcat
    59281 catgttgatc tggtacaagt ccgtgtgcaa tgtcaaacta tcatctggat acatactttt
    59341 cctacttcct tagctagaaa cccatgaaaa ttttcaagaa ctttcatgta ttccaataaa
    59401 ttagtactat tatatcacat tttagctgga ttgagaaaag agtaacaagc tattctccac
    59461 tctccaattc atccatatct tgttcaaatt ttttctgagc ccattcgcca tagctcttaa
    59521 gaccaagatt gccaataaag atccacggaa ggtaaatgat ataagtaatg acccaagcag
    59581 acaggtattt aaaattcaaa ggattgtgct gataaatttc tatgttgaat tgataattct
    59641 gcaacatcaa aagagccgta atagccaagg ttaggaaaaa acaacccaaa atcgtaaaat
    59701 gaaaacgact atagtaggtc actcccagat aacgggcacg attgaaaaag taaaatgtcc
    59761 ctatgatgat aacgattagc agcatattag aattaaaaag gcttggtgct aatactgaaa
    59821 tgatataaga taggagcgac aaagcgatgc agatatagaa actttcagag cccgctttat
    59881 tgaacagttg ttcttctctt tcgtctagta attgataata ataaaatcta tttttcatct
    59941 tcttcctccc aaaatagttg gtctagggtt ttccctaaac atctgcaaat ggactggcag
    60001 agcgagagac tgggattgta tttccccgct tctatcaaac caatagtctg gcgcgtcacc
    60061 ccgacagcct ctgccagttg accttgtgtt aaatcacgct ctacccgagc taattttaat
    60121 tttaaatttt tagccacctt cgtcctcctt atagttttaa tactcatcta ctcttaaaaa
    60181 atccaaaacc aacacaagct atcagctatt tattataacc ccattaattt gcactccagt
    60241 attatcttca gccgaaaatc agtattttag gttgtttgaa ataaacttcc tagtttactc
    60301 ttttattttt atttagtatt agctttagta ggatctttta aacgagtttt tattgcttat
    60361 atttcttgtt tagtttagtt ggtttcatat cactattata taatgctttt ggaaatgagc
    60421 ctagtataag tcaaaaacgt gttgaatagt gaaagcagga gcataccaag atgactgatt
    60481 tttttattgt attttaggta tgcaaaataa tactaaatga aactaaaatg aatacttctt
    60541 gcttttcttg agagtgaaaa agtacaataa aatgattgta gtgattagca caagatactg
    60601 actaatagac atcgtatttg aagccacaaa tacaggtaaa gaataaaaaa ggaagaagtt
    60661 caattgcttt gataaacaaa tcattactag tattcatcat catcgtattt aatatattag
    60721 agataaagag tagtaaagct actaaaatac caaataccaa gccacttttc aatttgtata
    60781 aatagaaaat gtaagataat agaaaataaa acaggactaa caaaattgta ttgactacaa
    60841 tagctactat aaaatttaat cctaaatcac ctggaatacc caatttactt actatgacta
    60901 gtaacacaat gccattcaaa gcataaataa tggaaactga gatagcatat agaaaattag
    60961 acaagacgta aatcatccga ttcttctgtt tatttaattt catgattata accttccttt
    61021 attttgaacg atttaggtaa atatctttaa gcgtctcttt tttagtttca aaatcaacta
    61081 ctttaataga gttttcgtta aaaaatttaa aaagctcact attttgaata ttcccagaca
    61141 tagtaattct caatccctct tcctaaacaa tatcccaaaa ttcttgtttg gtaatgaaaa
    61201 tgtctctatc tgtagctgat gaaaaagcta tctcaaacaa gaaattatga ctatcttttc
    61261 ctactttttg aaatgtcaaa agcccgttct ccaagaaaag aactctctca caaatttctt
    61321 caatgtcttc taatttatga ctcgatatta aaattcccac attttcatgt aaagctaatt
    61381 tttttagaac cgctaaaact atttgtgatg attcaatatc taaaccatta gtcggttcat
    61441 ctaaaatcaa tatatcaggt tccgtaacga gagttagaag caaagctaat ttttgttttg
    61501 tacccaaaga ataggtcttt acttttttat taatagactg agtcaaatct aactcttgga
    61561 tcaaacatct aaatctttct tgattgtagt caacaccata taaatttgac aaatatttta
    61621 aattctctaa acctgtttta gataaaaata attttggttc ttcgattaaa tacccaacat
    61681 tatcactgct tataatatta cctgaagtcg gttgattatt ctgaacaaga attttcatca
    61741 acgtactctt accaactcca tttctccctg ctagaccaac aatttcaccc ttgttaagtg
    61801 caaaatttat atctttgaga atgattgaat gaccataatg cttacttaca ttttttattc
    61861 ctccattaat attattggtt ttctctttga ttctcattaa ttttaaaaag ttgcgcctct
    61921 ttttaacaat attatatcat atatctaact taatgcaata tataaatgtc attttgtgaa
    61981 attttattaa caaaaaactc tctacaacaa aataatagag agttttctgc ttattaattt
    62041 ttttcaaaat atctaagaca tcacttcttt gtttaatttg ataggctttg atacctgagc
    62101 tccgacttta tcacttaaaa aataaaaaag acagctaatc tcttgcgcat caagggattt
    62161 cgccattctt taatctcgtt taagtgttgt atatggagct ttgcataatt ttttaaaact
    62221 aaacaactac attaagcacg aactgtgacg ctggttccat cttccttata aagattgata
    62281 agcccctctt taagagctcg aaccatgtct ccagcttgaa caggttgtgg gactttaata
    62341 gtcaatagtt ccattggatt tggagcgcgg tcgattttat tgcctttggc atcatgcaaa
    62401 tcttcaatat aggtttcaaa atgacggaaa cctggaccat aaaactcaac ttggtcccct
    62461 tcgttaatga cgttccgttg acgaatggtt gctgtttgtg tcgcatcatc ataagaaatc
    62521 acttcagcga caaacttgta ctcaggaatt ttacgacgag caccaaacaa ctgctcattt
    62581 tcagatggta taccatagta aaatcctgta gccagttcac gttgggcaac cttccacatc
    62641 tcgtccacca agtcttgttt gatagcttca aacttttcag gactttcaag ataggcatcc
    62701 acagccgcct tgtagcagtt ggttactgtt gatacgtagt gaatagactc cactccatac
    62761 gtccttcgat ttttagattg tccacaccat tttcaatcat atctggaatg tggtcaatca
    62821 tagacatgtc aacggctgac attgaaaatt cttctggaat ctcaccctgc aaactcttac
    62881 gttctttccc aaatggcata tcgtaaaggt cgtatttcca acggcatgac tgagaacatc
    62941 caccacggtt ggcatcacgc atactctaga cggattgttt tgacaattct aatcttttag
    63001 actttgcata ataactgtta aacagtcgaa aattacccca gttttaacaa gtagtcaaaa
    63061 taccagataa aggaaaaaag tctttaaaat cttaaaaacg catagtatca ggtattgaac
    63121 aatcttgata ttatgcgttt tattgtggga agatttactt cattttctct tgaaattgag
    63181 ttttctccta cttcaaaact agatgatttg ctatttaaaa tagcgttcct tatgattttg
    63241 gcaacctgga ttaaagcgag aaaaacaaaa ggggcaactt aaactttctt tatattgctt
    63301 atatgttagt agttttagac aaacaccaca taaaataggc ttatcctgta tcaaagataa
    63361 gggatacggc tcaaaagggt gattttctaa tgaatcatga caccgataac aagcataata
    63421 ctttttacaa tcatagcatt gtaaagcaat aatatccagc ttgctatggt aatgaatgca
    63481 tcttgtttgc ttatccacta agaaaccctg tgcctctatc atttcctttt cctttcttaa
    63541 gctcgaactg tgacgctggt tccatcttcc ttataaaaat tgatgagtcc ttcttttaat
    63601 gcacgaacca tgtctccagc ttgaacgggt tgtgggactt taatagtcaa tagttccatt
    63661 ggatttggag cgcggtcgat tttattgcct ttggcatcat gcaaatcttc aatataggtt
    63721 tcaaaatgac ggaaacctgg accataaaac tcaacttggt ccccttcgtt aatgacgttt
    63781 cgttgacgaa tggttgctgt ttgtgtcgca tcatcataag aaaccacttc agcgacaaac
    63841 ttgtactcag ggattttacg gcgagcacca aacaactgct cattttcaga tggtataccg
    63901 tagtagaaac ctgttgccaa ttcacgttgg gcaaccttcc acatctcgtc cactaagtct
    63961 tgtttgatag cttcaaactt ttcaggactt tcaagatagg catccacagc cgccttgtag
    64021 cagttggtta ctgttgatac gtagtgaata gacttcatac gtccttcgat ttttagactg
    64081 tccacaccat tttcaatcat atctggaatg tggtcaatca tagacatgtc aacggctgac
    64141 attgaaaatt cttctggaat ctcaccctgc aaactcttac gttctttccc aaatggcata
    64201 tcgtaaaggt cgtatttcca acggcatgac tgagaacatc caccacggtt ggcatcacgc
    64261 atactcatgt ggtttgaaag agtacaacgt ccagagtatg aaatacacat agctccatgg
    64321 acaaaggctt caatttcaac atctgtacgt ttgcggatct cagctaattc ttccattgaa
    64381 acctcacgcg ctaaaacgac acgagtcaag cctagctctt tccagaactc aagggtttca
    64441 tagttagtgg cactggcttg ggtagaaagg tggatttcaa ggcctggtgc ttcagtcgct
    64501 gcaatcataa tcaaggctgg gtcagatacg ataactgctg caatcccgat atcacgcagt
    64561 ttacggaacc actcaccagc accagcttca tttccttcgt gcataaccat attagccgct
    64621 acatagacct tggcaccata cttggccgca aactgcacgc cttcttccat ctgttcgaaa
    64681 gtaaagtttc ccgcacggct acgaagacca taggcctgac caccgataaa gacagcatct
    64741 gctccatact gaacagctac ctttagcttc tctaaagtcc ctgcaggtga taaaacctca
    64801 ggacgtttta atgtttttgt catgatttct cctatttgca atataaaagt ttgacatttt
    64861 tccttctcat tttatagtaa ataaaggata aaatcaagcc tagacggatt gttttgacaa
    64921 ttctaatctt ttagactttg cataataact gttaaacagt cgaaaattat cccagtttta
    64981 acaagtagtc aaaaagtcta gaaaagtaaa aaccagtatc caaagatact gactcgtaaa
    65041 aatacataaa ttagtcctta gatgactact cccactctgt tgctcctata atggaaaagg
    65101 attgatacag tctagaatat cgctttttta tttattattc tgatataaaa ctgttagaat
    65161 tgattttttg ttttataccc aaatatgaat agagacgtag ctaataagtt gaataacata
    65221 tttggaaaaa tgagaaatag caatattact agaactttta gaaacaatca atatttaaaa
    65281 agaaaaagct caaagaattt attaaaccat gttataatag atatagctat aattttattt
    65341 ataacaagag gatagaaatg accgaattag aaagaaaaaa tcgaaaaatt agctaagaaa
    65401 tattctgata acttaaacat caaagttcaa gagagagttc gtgaaatggc aaatgataat
    65461 aagagccatt atttgatata cagagtttta ggtatttcat ttgaagaagg agaaaatatc
    65521 gatttgtatc aaaataaagg tcgtttttta tacaaatatg ctggttcatt tttagaagaa
    65581 gctgcagtac tatcctttaa cgaaaaattt ggtacagaaa atacttaaaa agttaacatt
    65641 cctaattctg aaagtacaaa acctaagact tttgaaattg attgtttagt cggagaaaaa
    65701 cacgcatacg aaataaaatg gtgggatgca actacagatg gagaccatat aactaaagaa
    65761 cacactagaa taaaagttat tcataacaaa ggatatatac caattcggtt aatgttctac
    65821 tatccaaata gaactcaagc tataaaaatt cagcaaactt tagaaacatt gtataacggt
    65881 attggaggga aatattatgg agattctgcc tgggaagatt taagagcagt gaccggtatt
    65941 gatttactta gtattctaac agatattgca aataaaaaac aggggtaaaa tcaaaatgac
    66001 agtattaaaa ggagataact tagaaatatt aaaaactatt gaatcctcaa gtattgattt
    66061 aatctatatg gaccctcctt tctttacaca gaaaacccaa aaattatcta ataacaaaaa
    66121 tattatgtat tcattcgaag atacgtggac ttcgattgag gattacaaag aatttttgtc
    66181 tgtaagatta gaagaatgca aaagagtgct aaaaaatagt ggcagtattt tcgttcattg
    66241 tgataaaatt gcaaatcatc atattagatt aattttagat aatatctttg gagcagatat
    66301 gtttcaaagc gaaattatat ggaactataa acggtggtct aattcaaaaa agggattatt
    66361 gaacaatcat caaaacattt acttttattc aaagtcaaaa gattttaaat ttaatacaat
    66421 ttttacagag tattcttcta ctacaaatat cgaccaaata ctagtggaac gaaaacgaga
    66481 tggaaactct aaaactatat ataaagttga taataatggt aactatattc tagcaaaaga
    66541 gaaaaatgga gttccccttt cagatgtttg gaatatacca tttcttaatc caaaagctaa
    66601 agaaagagta ggttatccta cacaaaaacc tattctgtta ttagaacaaa ttataaagat
    66661 tgctactgat aaaaatgata tagttttaga cccgttctgt ggaagtggaa ctactttagt
    66721 agcctccaag attttgaata gaaattatat ggggattgat ttatctgagg aagctatcaa
    66781 tataactcag caacgtctgg aaaatgttat aaaaacaagt tcagatttat tgaataaagg
    66841 aatcgaagca tatagaacca aaactgagga agaggaaaac attcttaaat tattacaggc
    66901 aaaaattgtt caaagaaata aaggaattga tggtttttta cctaaacatt ttcaaaaaaa
    66961 accgatacct ataaaaattc aaaaaaataa tgaatgtctg aatgagagta tctctttatt
    67021 acagaatgct ataaactcca aaaaacttga ttttggagta gttataaaaa ctcattcaga
    67081 taatttatta tttgattttg atacgattcc tgaaaatata atagtagttg accattttga
    67141 acttacaatc gaaaaatggt taagtaaaag tcaacagtta ctatagtttg caaattattt
    67201 acagaaagaa caaaaaatgc tctgaaacac tgatttcaag gcattttttg ttagtttaaa
    67261 attactccca ttcttctttt ccaaacatac aatatatcca aaaccattca aaatactaga
    67321 ttctattttt tataatatca ctaaatccac ctaattatag gacgttttca gatttttagt
    67381 cccgtcccag tcccagtacc ggagaaatat tgttttaata taatatctct ttttgtcttc
    67441 taagctctta aaagcaaaag aacaagtaaa gagtcaagac aaggataaaa agtccatatt
    67501 agggcaaata aaaagcttta agacagatga caaatctaag tcaaataaga aagaccatag
    67561 caaaggtgca gagagataaa tattggcggt cttcggactg cctttatttt tttatccatt
    67621 tttcaaatca aatttattca gactatatat gcacatatac acttaaattc atataaaaac
    67681 atggcttgta aaaaattact ttaatcacaa taatcgcatt taaaattgtg atgtttgcaa
    67741 gctaaattac ggacttcact tggaagtttt cccttgtatc ttttataata gatagaaaat
    67801 ttgctggcag atgaatatcc aacagattct gctatctctt ttataggtag ttcagtgttt
    67861 aaaagaagag tttcagctac attcattctt tttctttgag tgtactctgt aatgctttga
    67921 caatattttt ccttaaataa attttttaat ttagtaccac tcattttaga tattttttca
    67981 agcgtgcctt gatttacatt cgttgcaaaa tgatcatcta agaatcttgc tacatcttca
    68041 agtgctttat catcatcaat ttcaatttta tatttttttc tatttaagta tgtgtcaatt
    68101 actatactta tccattcatt tgcctttgct ttaaagaaaa aatcagcggc aggagcgtcc
    68161 atcttacaat ttaatatttc cattgccact ctttctaagg cctttgtaag tattatttga
    68221 ttcgattgaa gcaaggttga ataaaaagat tctggattaa tgttaataga tgctaaatgt
    68281 ttttctatta gctctttttt aaaaccaatg gaaacagcaa gataacaaca attctcgtgt
    68341 aataaaaaaa caaaatcatc ttttatatta tcaaaatcaa aagtacataa agagtttgcg
    68401 gtaatagttt gatacggatt aaacttttct ccgtttgcac tgacaatgta acttgaataa
    68461 attgaaacat agtctgacat actataagtg ctattttgaa ctacttcctc tttgatataa
    68521 aaatcatgta tatcgataat gaagctgcct ccttcataaa accaatatag gccttctgca
    68581 taagttgaat tctctttttt ccaacaaaat gtatgacctg cacttgaata cttctcattg
    68641 tttttacatt catctacttt catataatct tttacaaaat cataatatga cataacacac
    68701 tatccctttt agacaatatt ccaattagcc ttattaattc aaaactattg tattagtaat
    68761 tataacagat gtataataga aaagcaatga tagatattat caattaagcg aatttatatc
    68821 taaaagggat attaaagaaa ggagatatgc ttatgaagat ttacaaaaaa ctatttgctt
    68881 atgtccaaga taagaaatat cttggggttt tggccataat tttttctgct atatctgctg
    68941 cacttacagt atatggatat tatttaatct acaaatttct agataagtta ataattaatt
    69001 caaacttatc cggtgcagag agtatagcat taaaatctgt tattacacta acaagtggag
    69061 cgatatttta ttttgtctca ggaatgtttt cacatatctt gggatttagg cttgaaacaa
    69121 atttaagaaa aaggggaatc gatggtctgg aaaaagcaag ttttaggttc tttgacttaa
    69181 atccatctgg tcaaataaga aagatcatag atgataatgc agcacaaact catcaggtgg
    69241 tagcacacat gattcccgat agttctcagg caataatcac acccgtactt gtacttgcac
    69301 ttggctttat agtaagtata agggttggca taattttgct tgctcttact ataattggtg
    69361 gcttaatttt aggggcaatg atgggcgagc aagaatttat gaagatatac caagaatccc
    69421 tatctaaact aagtgctgaa actgttgagt acgtgagagg aatgcaagtt gtaaaaatat
    69481 ttaaagcaaa tgtagagtct tttaaaagct tttataaggc gataaaagat tactcaaagt
    69541 atgcttatga ttattcccta tcttgtaaaa ggccttatgt tttgtatcaa tggttatttt
    69601 ttggactgat tgcaatttta attattccta tagtttattt tatgactagc ttagctagcg
    69661 caaaggtgat tttacttgag cttatcatga ttttattttt atcaggagtt ctctttgttt
    69721 cattcatgag aatgatgtgg tactccatgt atatttctca aggaaattat gcagtagata
    69781 ctttagaggc gctttacgaa gatatgcaaa aagacaaatt agtgcatggt aatgtcaata
    69841 attttaaaaa ctataatata gaatttgaga atgttagctt tgcttataat gataaagctg
    69901 tcattgaaaa tttatccttt aatttagaag aaggaaagtc ctacgcactt gtcggttcat
    69961 ctggatcagg caaatcaaca gtagcaaaac ttatatcagg tttttacaat gttaataaag
    70021 gaagcataaa gataggcggg atagcaataa gtgaatattc tgacgaagcc ttaattaaag
    70081 ccatttcctt tgtttttcaa gattcaaaat tattcaagaa gagcatttat gataatgtag
    70141 ctttagctaa taaagatgcg acgaaagatg acgttatgag agccttaaaa ttagcaggat
    70201 gcgatttaat attagacaaa ttcccagaaa gagaaaatac aatcataggc tcaaaaggtg
    70261 tttatttatc cggtggagaa aaacaaagaa ttgcaattgc tagagcaatt ttaaaggatt
    70321 ccaaaattat tattatggat gaagcatcag catctattga cccagataac gagtttgaat
    70381 tgcaaaaagc ttttaaaaat cttatgaagg ataaaacagt tatcatgatt gcacacaggc
    70441 tatctacaat taaagacctt gatgaaatta ttgtcatgga tagtggaaaa attatagaaa
    70501 gagggtctga caaagaatta atgtcaaaag atacaaggta taagagcctg caagagatgt
    70561 ttaacagtgc gaatgaatgg agggtttcaa atgaaagagt tttataaaaa aagatttgct
    70621 cttacagatg gaggagcaag aaatttaagt aaagcaacac tggcttcatt tttcgtttat
    70681 tgtataaaca tgcttcctgc catattactt atgatttttg ctcaggaagt tttggaaaat
    70741 atgggcaaaa gcaatggctt ttatatagta ttctcagttt tgactttgat agcaatgtat
    70801 attttgcttt ctatcgaata cgataaatta tataacacaa cctatcaaga aagtgcagat
    70861 ttaagaataa ggacagcgga gaatttatca aaattacctc tatcttactt ttctaaacat
    70921 gacatttccg acatttcaca aacaatcatg gctgatattg aaggcataga gcatgcaatg
    70981 agccactcaa taccaaaggt gggcggcatg gtactgtttt tcccattaat atctgtaatg
    71041 atgctagcgg gcaatgtcaa gatgggttta gctgtaatta ttccatctat tttaagcttt
    71101 atatttatac ctttatctaa aaaatatcag gttaatggac agaatagata ttatgatgtc
    71161 ttaagaaaaa actcagaaag ctttcaagaa aatatcgaaa tgcaaatgga gattaaagca
    71221 tataatttat cgaaggatat taaagatgac ttatataaaa aaatggaaga tagtgagaga
    71281 gtacacttaa aggcggaagt aactacaatt ttaactttgt ctatatcttc aatatttagc
    71341 tttatatctc ttgctgttgt gatatttgtc ggcgtaaatc taattattaa taaagagata
    71401 aattctctct accttatagg atatttacta gctgctatga agataaaaga ctctttagat
    71461 gcatctaaag agggcttgat ggaaatattt tatttatcgc ccaaaataga aagattaaaa
    71521 gaaattcaaa atcaagatct acaagaaggc gatgactata gcttaaaaaa atttgatatt
    71581 gatctaaaag atgttgagtt tgcctacaat aaagacgcaa aagttttaaa tggtgtaagt
    71641 tttaaagcta agcagggaga ggtcactgct ttggtaggtg caagtggctg cggtaaaaca
    71701 actatcttga aacttatatc aagactttat gattatgaca agggacaaat cttaatcaat
    71761 ggcaaagata taaaagaaat atcaacagaa tctctttttg ataaggtctc tatagttttc
    71821 caagatgtgg ttctctttaa tcaaagcgtt atggaaaata ttagaatcgg aaagcaagat
    71881 gcaagtgacg aagaggttaa aagagcagca aaacttgcaa attgcacaga ttttatagaa
    71941 aaaatggata aaggtttcga tacagttatt ggtgagaacg gagctgagct atcaggagga
    72001 gaaagacaaa gattatcaat agccagagcc ttcttaaaag atgcgccgat attgatctta
    72061 gatgagataa cagcaagcct tgatgttaac aacgagaaaa agattcaaga gtctttaaat
    72121 aatttagtta aagataaaac tgttgtaatc atttcacata gaatgaaatc catagaaaat
    72181 gcagacaaga tagtagttct tcaaaacgga agagtagaaa gcgaaggtaa gcatgaagag
    72241 cttttacaaa aatcaaaaat ttacaaaaat ttaatagaaa agacaaaaat ggcagaagaa
    72301 tttatttatt aggaggacta caatggataa taaaaaatta aaagtaaaag atttagtaag
    72361 catcggtgtt tttggcgtaa tttattttgc cttcatgttt ggagttggta tgatgggctt
    72421 gattccaata ttgttcttaa tatacccgac agtattagcc atagttgcag gaactgttgt
    72481 tatgttattt atggctaagg ttcaaaagcc atgggcacta tttatatttg gtatgatatc
    72541 accacttgtg atgtttgcag ctggtcatac ctacgtagtt gtggttttat cacttatagt
    72601 aatgataata gcagaattaa ttagaaagat tggtaattat aattcattta aatacaatat
    72661 gctttcttat gcaatcttca gcacatggat atgtagctct ttaatgcaaa tgcttttagc
    72721 aaaagaaaaa tatatggagt ggtctttgat gacgatggga aaagattatg ttgatgtatt
    72781 agaaaagtta ataacttatc ctcacatggc tttagtagcc ttaggtgctt tcttaggagg
    72841 aattcttgga gcatatatag gcaaggctct attgaaaaaa cactttgaaa aagcaggcat
    72901 tgtataatgc ctactttttc ttcaaatgct ccctttaatc ccaatccgat ctctaagata
    72961 ttagtcgtat ttatcacagg cttaactgtt atgcatagca taaacatccg ttttgagctg
    73021 gcaattgttt gtcttattgg tattttgctt tatttgaatg gatacaaaaa aacacttttc
    73081 aaatggatta tattgtgtgg gatactttac tccttgccta attttatggt gttatctgaa
    73141 ttaaacccta tagttaagat gtttttgagt atacctattg ttattagaat gtttatttta
    73201 ccatttatgg cagcaagctt tatgataaag acctcggatg taggcgcaat aatttcatcg
    73261 atggataagc ttaagatttc aaagaatgta tccataccta ttgcggttat gtttagattc
    73321 ttcccatctt ttaaggagga gaagaaaaac atcaaaatgg ctatgagagt aagagggata
    73381 aattttaaaa acccagtcaa atatcttgaa tatgtttctg tgccactact cattatatca
    73441 tctaatatat cagatgacat tgcaaaagcg gcagaaacaa aggcaataga aaatccaatt
    73501 gccaagacca gatacattcg cgtaaagata cagctaattg attttgttta tgttttagcg
    73561 gttgttggac ttattgtggg aggcttaata tggttgaaat aaaaaattta agtcttgatt
    73621 atggtgaaga gcatatatta gatgatatat cactatccat agccgaggga gagtgcgtgc
    73681 tatttacagg aaaaagtgga aatggtaagt catctttaat aaattcaatc aatggactag
    73741 ctgtaaggta tgacaacgca aagacaaagg gcgaaatcat tattgatggt aagaatataa
    73801 aaaatttgga actttatcaa atctcaatgc ttgtttcaac tgtttttcaa aatcctaaga
    73861 catatttttt taatgtcaat acgacattag aattattatt ttatttggaa aatatcggtc
    73921 ttgcaagaga agagatggac aggcgtttga aggatatgct taagatattc ccgataaaaa
    73981 atcttttgaa cagaaatata tttaatctat ccggcggtga aaaacaaatt ctttgcattg
    74041 cagcttctta tatagcaggt acaaagatta tagttatgga tgagccttca tcgaatttag
    74101 atattaaaag cataagtgtt ttggcaaaga tgctaaagat attaaaagag aaaggtataa
    74161 gcataattgt tgcagagcat agaatttatt atttgatgga catagttgac cgtgtatttt
    74221 taatagataa aggaaagctt aaaaaaactt atactagaag tgaattttta aagctagata
    74281 aaaatgaatt aaatgcttta agtttaagag ataaagaatt aagtaaatta aaagttcctt
    74341 atttaaaaga aggtggagag tatcagataa aaaatcttag ctacaaattt actgatgatg
    74401 agtgtttaag cttaaaagat atttcattca agcttgggaa aatttatggc ataataggat
    74461 ccaacggacg aggaaaatca acgcttttaa gatgtttaat aggtcttgag aaaaaatcaa
    74521 aagaagaaat ttattttaag ggagagaagc tatctaaaaa agaaagactc aaaaactctt
    74581 cacttgttat gcaagatgta aatcatcaat tattcacaga tgaagtattc aacgagctta
    74641 gattaggagt aaagaatttt gatgaagaaa aggcgaaaat cattttaaaa gatttaggcc
    74701 tggacgaatt tattgaaagg catccgatga gtttatcagg agggcaaaag caaaggcttg
    74761 caatagcatc tgttatgtgc aagacttctc catttgtctt ttttgacgaa ccttcaagtg
    74821 gtatggatta ttccaatatg ataaaaatat ctgaactgat taataagtat aaaaccatgg
    74881 ataaaataat ttttattgtt tcccatgata tagaattttt aaatgaagtg gcagatgaaa
    74941 tttttgaatt gtaaaataga gtctgttaaa catgtctata gaactagtaa attgtctaaa
    75001 aatttaaaag atactaacac atattgatat ttattagaaa attaacttca ggatttttac
    75061 agtatttata aactttataa tatgtattta tagtattaat aatttccata tctttaggga
    75121 aattctttat ttacttacca taattaaaga gatgagagat aaatatcggc agtcttcgga
    75181 ctgcctttat ttttttactt aatttaatgc agtaaatcat acgattgaaa ggaatctgcg
    75241 ttatatgcta taattaggtg gttaatctat acatttagaa aggtaaaata tagaattgga
    75301 gaatgatatg ggattttcat ataataaatt gtaaccgtcc aataacgaag tatattgaaa
    75361 aatctccaga ctagagaact cacgga
//
LOCUS       DAVBNO010000002        72426 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVBNO010000002 DAVBNO010000000
VERSION     DAVBNO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 72426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72426
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.002"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(840..998)
                     /locus_tag="VQ846_000078"
     CDS             complement(840..998)
                     /locus_tag="VQ846_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074747.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(1008..2318)
                     /gene="obgE"
                     /locus_tag="VQ846_000079"
     CDS             complement(1008..2318)
                     /gene="obgE"
                     /locus_tag="VQ846_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET3074748.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEETYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            complement(2382..2516)
                     /locus_tag="VQ846_000080"
     CDS             complement(2382..2516)
                     /locus_tag="VQ846_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET3074749.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            complement(2575..2751)
                     /locus_tag="VQ846_000081"
     CDS             complement(2575..2751)
                     /locus_tag="VQ846_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET3074750.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            complement(2782..4107)
                     /locus_tag="VQ846_000082"
     CDS             complement(2782..4107)
                     /locus_tag="VQ846_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET3074751.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAISEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            complement(4123..5166)
                     /gene="cpoA"
                     /locus_tag="VQ846_000083"
     CDS             complement(4123..5166)
                     /gene="cpoA"
                     /locus_tag="VQ846_000083"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET3074752.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPATLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            complement(5281..5610)
                     /locus_tag="VQ846_000084"
     CDS             complement(5281..5610)
                     /locus_tag="VQ846_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET3074753.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            complement(5624..6733)
                     /gene="rpoD"
                     /locus_tag="VQ846_000085"
     CDS             complement(5624..6733)
                     /gene="rpoD"
                     /locus_tag="VQ846_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET3074754.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            complement(6736..8496)
                     /gene="dnaG"
                     /locus_tag="VQ846_000086"
     CDS             complement(6736..8496)
                     /gene="dnaG"
                     /locus_tag="VQ846_000086"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET3074755.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKIIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKASEIYLMEGFMDVIAAYRVGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VKEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            complement(8586..9344)
                     /locus_tag="VQ846_000087"
     CDS             complement(8586..9344)
                     /locus_tag="VQ846_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3074756.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQVTIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            complement(9341..10207)
                     /locus_tag="VQ846_000088"
     CDS             complement(9341..10207)
                     /locus_tag="VQ846_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3074757.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            complement(10276..11280)
                     /locus_tag="VQ846_000089"
     CDS             complement(10276..11280)
                     /locus_tag="VQ846_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET3074758.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNITGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     misc_binding    complement(11393..11610)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11693..14389)
                     /gene="ppc"
                     /locus_tag="VQ846_000090"
     CDS             complement(11693..14389)
                     /gene="ppc"
                     /locus_tag="VQ846_000090"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET3074759.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATNLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(14409..15638)
                     /locus_tag="VQ846_000091"
     CDS             complement(14409..15638)
                     /locus_tag="VQ846_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET3074760.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSATSVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(15794..16096)
                     /locus_tag="VQ846_000092"
                     /pseudo
     CDS             complement(15794..16096)
                     /locus_tag="VQ846_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(16544..16765)
                     /locus_tag="VQ846_000093"
     CDS             complement(16544..16765)
                     /locus_tag="VQ846_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3074761.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTDSAYAKIERGEHTLTADI
                     LVTLSNFYDVSTDYLLGLTDFPDKIRFRK"
     gene            complement(17445..18656)
                     /locus_tag="VQ846_000094"
                     /pseudo
     CDS             complement(17445..18656)
                     /locus_tag="VQ846_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(18658..19902)
                     /locus_tag="VQ846_000095"
     CDS             complement(18658..19902)
                     /locus_tag="VQ846_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HET3074762.1"
                     /translation="MHIVLFRYSARVVKLKYLYNRNVVIIAPEVQRKKYEDYLAEGNN
                     LPKVIFLEDFILPNIILEIRRISQNTEVESITTLSEEDMDIVGFLNDHFVEKNTHSVS
                     NLLFKDKYFMRSFLVGVVNQPYFRLLESENDLRVFWNNCKSSAAILKPRNEAGSNGIK
                     KVYPNEKIDSVYFEKNYIIEEYIDIDNMITCDGYSIGSEIKRFYVHEYEELLFNTLSS
                     SGYYLVRTSSLYNDSEKIEYIEKALEECRKVLNVFSINSEITPFHFEWFLELSTGKIV
                     FCEVGKRFGGGDIPQLIEDSHGVDVIKEYWDILTNSQVLKKINFSKVISRPANISATF
                     ALYRKQGKVIEVPDEKELAWAKKVYIFIKPNTYYKVSENVVENSMFVQFNCKNSCEFE
                     KRLDELKHISQKFKYVITDSGE"
     gene            complement(19932..20960)
                     /gene="galE"
                     /locus_tag="VQ846_000096"
     CDS             complement(19932..20960)
                     /gene="galE"
                     /locus_tag="VQ846_000096"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET3074763.1"
                     /translation="MKSLLITGGAGYIGSHTVLDLLDNNYEVTIIDDLSNSKKKVIES
                     IKQISKKSRNLHFYKIDLKNEEKLENVFKRHNFDGVIHFAGYKAVGESVVEPLKYYEN
                     NLLSTINILKLMKKYKVFNFVFSSSATVYESTPIMPFYETNPLKASNPYGRTKQYIEV
                     LLNDLFISNSNWKIVCLRYFNPLGAHESGDLGEDPNGIPNNLVPYITQVAIGKLPYLN
                     IFGVDYSTPDGTCIRDYVHVNDLADGHRKALEYIFNTDEGLYEVINLGSGVGFSVFEI
                     LHSLESVIGSYIPYKITSRRAGDMDVSIADISKAEELLGWKPRYDIMKMCQDTWKWQQ
                     KHPNGYDD"
     gene            complement(20962..21633)
                     /locus_tag="VQ846_000097"
     CDS             complement(20962..21633)
                     /locus_tag="VQ846_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074764.1"
                     /translation="MIRLPNKFKGNNITLEVKLNKAVDASVLYPDVKSLDQELYRQLQ
                     SVESFYLTFSDMYSEINENNEILYTEIIFKIPLCIGEDYFNFPVVSYVSNPYSLIRGF
                     YLGFHKELDCNYVFDDVELHFFREGICEFNFEFKNQNSIITAELPREYNYPLILFNNS
                     NLVNQYGKYTLLDIDKYKLESRKIFEFPEISIGNFLGSEAKSNRVIFSQDKFQINGIK
                     NMEVN"
     gene            complement(21636..22730)
                     /locus_tag="VQ846_000098"
     CDS             complement(21636..22730)
                     /locus_tag="VQ846_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074765.1"
                     /translation="MFSEIIKKYDIVTLKDSGFLRGDWFIGSALMAHDYESLKEGIYF
                     YPQETIWTRILDLEYINPYWEIYNQTYNFLLVNKQDSLAMNFLTSLLREIEVISSKTL
                     DFVVITSNEHLIRNLIDRDYYIITKEIPFFIENSKVGNYFKIFVKNGGQFIPIIDCTE
                     FKIQSKKYFEININKFYLKLLDYFLHGEIPEKFKFFGNTSNFNIESLFLEFIDSQENI
                     RVVSLLDCLIQLSSKNIEISGNYKGHTVKKILKKFALYSVITKLDITKVNHLLRNDYI
                     YFLMKEIDNITKKYPKLIQKYPRDILSDRYGITNEVYRYLIGENLHIKINHKLDEVRY
                     FTDIPFIDTSLSIEEFDKIVSKILKIKVGG"
     gene            complement(22734..23264)
                     /locus_tag="VQ846_000099"
     CDS             complement(22734..23264)
                     /locus_tag="VQ846_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074766.1"
                     /translation="MIINTLGPTATDCFYAINRIKLKVDVIVLYDSFDSIVNCFDKLK
                     GQYILLPVAFESSIKNYSWKDFNFEYHKRIEIVDSFHSFTKGMVLIENCNYKINRAII
                     QPATEILMLNYLKKKSMDLRIDFTHSKVMANKKFLEEGYRFSIVSQETIINNDDIIIC
                     ETFSPEMIWCLYRVKG"
     gene            complement(23261..23983)
                     /locus_tag="VQ846_000100"
     CDS             complement(23261..23983)
                     /locus_tag="VQ846_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET3074767.1"
                     /translation="MITYRNDLLSKELDILISFFKKCEVGKISLVITGSLARGNPRIK
                     DGKLESDIDILVIVDSIQQLISIKKTLEGRFHFVHKISLIFCLKERINRSRYRGIINS
                     IRSVDNLLVDNLHIKNQIIEALDSPTNIVEQTRSMIQEFCYYSSKYLISKNNYLELKL
                     EKYWKEIATLNHIDKKIKHLDFERIFAVLKEHKIQILDSSEYFFQNVKTSENIYLEMR
                     DLVSLENQGLDFEHCILSLGER"
     gene            complement(23970..24839)
                     /locus_tag="VQ846_000101"
     CDS             complement(23970..24839)
                     /locus_tag="VQ846_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HET3074768.1"
                     /translation="MKHKKVLIIGYGIMTRGILKILKQSKKYNIYLYSRHLNSTINGI
                     IFIREDELPDAINQVDFIITCFSNHIETDNFINEFLLNIQSLRKTIVIDFTTSRIDCV
                     EKFKKTVEIKGGYYIEAPFTGGKLGSNSGQLSIFLHIDEGVNYNKKELMELLKLISKK
                     IYFFEKSTEPTKFKLIYNSWGAYLLYSIKLFNPLQYDFSPKSLEVANTIVCNDGWMSL
                     VCNSKLEQINDQDFDDIHFRVEYMVKDLEYANFSVFHDEVKEINYLLQQYRDCLVTDS
                     GKDYTVIGKYDNL"
     gene            complement(24943..25014)
                     /locus_tag="VQ846_000102"
     CDS             complement(24943..25014)
                     /locus_tag="VQ846_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HET3074769.1"
                     /translation="MKKISKFFPILMLVMDIIIIVGG"
     gene            25096..25948
                     /locus_tag="VQ846_000103"
                     /pseudo
     CDS             25096..25948
                     /locus_tag="VQ846_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
     gene            complement(25993..27857)
                     /locus_tag="VQ846_000104"
                     /pseudo
     CDS             complement(25993..27857)
                     /locus_tag="VQ846_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261806.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(27844..28142)
                     /locus_tag="VQ846_000105"
                     /pseudo
     CDS             complement(27844..28142)
                     /locus_tag="VQ846_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480038.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
     gene            complement(28218..28577)
                     /locus_tag="VQ846_000106"
     CDS             complement(28218..28577)
                     /locus_tag="VQ846_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074770.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIVE
                     VNQAQPLSREFCEKYMG"
     gene            complement(29396..30553)
                     /locus_tag="VQ846_000107"
     CDS             complement(29396..30553)
                     /locus_tag="VQ846_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001213952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074771.1"
                     /translation="MRGFNNKIKSVYQELTNSKEKFGSFHKTLIHLHTPVSYDYKLFS
                     NWTATKYRKITEDELYDIFFENKKIKVDKTIFFSNFDKVVFSSSKEYISFLMLAEAII
                     KNGIEIVVVTDHNTTKGIKKLQMAVSIIMKNYPIYDIHPHILHGVEISAADKLHIVCI
                     YDYEQESWVNQWLSENIISKKDGSYQHSLTIMKDFNNQKIVNYIAHFNSYDILKKGSH
                     LSGAYKRKIFSKENTRFLEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYS
                     DYSKDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQV
                     VIMESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            complement(30581..31342)
                     /locus_tag="VQ846_000108"
     CDS             complement(30581..31342)
                     /locus_tag="VQ846_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HET3074772.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPKLSYLSTLIRYEE
                     LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLHELLFGEWSQVEKEMLRVGEKRLNELLEK"
     gene            complement(31342..31818)
                     /locus_tag="VQ846_000109"
     CDS             complement(31342..31818)
                     /locus_tag="VQ846_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3074773.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            complement(32263..32718)
                     /locus_tag="VQ846_000110"
     CDS             complement(32263..32718)
                     /locus_tag="VQ846_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074774.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNAIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            complement(32993..34730)
                     /locus_tag="VQ846_000111"
                     /pseudo
     CDS             complement(32993..34730)
                     /locus_tag="VQ846_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            complement(34893..35777)
                     /locus_tag="VQ846_000112"
     CDS             complement(34893..35777)
                     /locus_tag="VQ846_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET3074775.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLINKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(35889..36743)
                     /locus_tag="VQ846_000113"
     CDS             complement(35889..36743)
                     /locus_tag="VQ846_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET3074776.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(36801..37559)
                     /locus_tag="VQ846_000114"
     CDS             complement(36801..37559)
                     /locus_tag="VQ846_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET3074777.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            complement(37621..37809)
                     /locus_tag="VQ846_000115"
     CDS             complement(37621..37809)
                     /locus_tag="VQ846_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET3074778.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(38133..38699)
                     /locus_tag="VQ846_000116"
     CDS             complement(38133..38699)
                     /locus_tag="VQ846_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074779.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(38967..40646)
                     /locus_tag="VQ846_000117"
                     /pseudo
     CDS             complement(38967..40646)
                     /locus_tag="VQ846_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(40738..40899)
                     /locus_tag="VQ846_000118"
     CDS             complement(40738..40899)
                     /locus_tag="VQ846_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074780.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(41583..41897)
                     /locus_tag="VQ846_000119"
     CDS             complement(41583..41897)
                     /locus_tag="VQ846_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074781.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            complement(41863..42252)
                     /locus_tag="VQ846_000120"
     CDS             complement(41863..42252)
                     /locus_tag="VQ846_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET3074782.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            complement(42640..43434)
                     /locus_tag="VQ846_000121"
     CDS             complement(42640..43434)
                     /locus_tag="VQ846_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3074783.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            complement(43434..44441)
                     /locus_tag="VQ846_000122"
     CDS             complement(43434..44441)
                     /locus_tag="VQ846_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET3074784.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            complement(44441..45448)
                     /locus_tag="VQ846_000123"
     CDS             complement(44441..45448)
                     /locus_tag="VQ846_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET3074785.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            complement(45438..46463)
                     /locus_tag="VQ846_000124"
     CDS             complement(45438..46463)
                     /locus_tag="VQ846_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET3074786.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            complement(46856..47074)
                     /locus_tag="VQ846_000125"
     CDS             complement(46856..47074)
                     /locus_tag="VQ846_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3074787.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(47320..47598)
                     /locus_tag="VQ846_000126"
     CDS             complement(47320..47598)
                     /locus_tag="VQ846_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074788.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSWYAAYNIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(47812..49443)
                     /gene="rlmD"
                     /locus_tag="VQ846_000127"
     CDS             complement(47812..49443)
                     /gene="rlmD"
                     /locus_tag="VQ846_000127"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET3074789.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHDLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(49517..50491)
                     /locus_tag="VQ846_000128"
     CDS             complement(49517..50491)
                     /locus_tag="VQ846_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET3074790.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(50565..51179)
                     /gene="pmp23"
                     /locus_tag="VQ846_000129"
     CDS             complement(50565..51179)
                     /gene="pmp23"
                     /locus_tag="VQ846_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET3074791.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(51179..52153)
                     /locus_tag="VQ846_000130"
     CDS             complement(51179..52153)
                     /locus_tag="VQ846_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET3074792.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(52161..53417)
                     /locus_tag="VQ846_000131"
     CDS             complement(52161..53417)
                     /locus_tag="VQ846_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET3074793.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLAAGGHLTHGAPVSFSGQTYNFLSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEDLAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(53479..53910)
                     /locus_tag="VQ846_000132"
     CDS             complement(53479..53910)
                     /locus_tag="VQ846_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3074794.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(53912..54511)
                     /locus_tag="VQ846_000133"
     CDS             complement(53912..54511)
                     /locus_tag="VQ846_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET3074795.1"
                     /translation="MDRIRQELENGGAVILPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNVASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(54498..55337)
                     /gene="prmC"
                     /locus_tag="VQ846_000134"
     CDS             complement(54498..55337)
                     /gene="prmC"
                     /locus_tag="VQ846_000134"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET3074796.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(55337..56416)
                     /gene="prfA"
                     /locus_tag="VQ846_000135"
     CDS             complement(55337..56416)
                     /gene="prfA"
                     /locus_tag="VQ846_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET3074797.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(56426..56905)
                     /locus_tag="VQ846_000136"
     CDS             complement(56426..56905)
                     /locus_tag="VQ846_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3074798.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVATLAVYDNDAIIGFVMIAYDKKIGISSGNYLLFRFMIDRNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEIAENEVVAIYDLTIEK"
     gene            complement(56895..57494)
                     /locus_tag="VQ846_000137"
     CDS             complement(56895..57494)
                     /locus_tag="VQ846_000137"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET3074799.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFTPMITSNKEQNYDN"
     gene            57617..57799
                     /locus_tag="VQ846_000138"
     CDS             57617..57799
                     /locus_tag="VQ846_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET3074800.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            57936..59309
                     /gene="mnmE"
                     /locus_tag="VQ846_000139"
     CDS             57936..59309
                     /gene="mnmE"
                     /locus_tag="VQ846_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET3074801.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(59356..60291)
                     /gene="dapA"
                     /locus_tag="VQ846_000140"
     CDS             complement(59356..60291)
                     /gene="dapA"
                     /locus_tag="VQ846_000140"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET3074802.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(60347..61423)
                     /locus_tag="VQ846_000141"
     CDS             complement(60347..61423)
                     /locus_tag="VQ846_000141"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET3074803.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(61765..61837)
                     /locus_tag="VQ846_000142"
     tRNA            complement(61765..61837)
                     /locus_tag="VQ846_000142"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61802..61804),aa:Thr,seq:ggt)
     gene            complement(61937..62446)
                     /locus_tag="VQ846_000143"
     CDS             complement(61937..62446)
                     /locus_tag="VQ846_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET3074804.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(62444..62540)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            62569..63000
                     /locus_tag="VQ846_000144"
     CDS             62569..63000
                     /locus_tag="VQ846_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET3074805.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            63082..63459
                     /gene="mscL"
                     /locus_tag="VQ846_000145"
     CDS             63082..63459
                     /gene="mscL"
                     /locus_tag="VQ846_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET3074806.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(63501..64595)
                     /gene="hemH"
                     /locus_tag="VQ846_000146"
     CDS             complement(63501..64595)
                     /gene="hemH"
                     /locus_tag="VQ846_000146"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET3074807.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVTLVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRSPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            64750..65973
                     /gene="pepT"
                     /locus_tag="VQ846_000147"
     CDS             64750..65973
                     /gene="pepT"
                     /locus_tag="VQ846_000147"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET3074808.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNAELGSYRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(66009..66638)
                     /locus_tag="VQ846_000148"
     CDS             complement(66009..66638)
                     /locus_tag="VQ846_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074809.1"
                     /translation="MSKVNQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIEIASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            66603..66824
                     /locus_tag="VQ846_000149"
     CDS             66603..66824
                     /locus_tag="VQ846_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074810.1"
                     /translation="MDLFQELIYLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(67095..68106)
                     /locus_tag="VQ846_000150"
                     /pseudo
     CDS             complement(67095..68106)
                     /locus_tag="VQ846_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(68332..71451)
                     /gene="phtE"
                     /locus_tag="VQ846_000151"
     CDS             complement(68332..71451)
                     /gene="phtE"
                     /locus_tag="VQ846_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET3074811.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKKLSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPGN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLPETGNSTS
                     DSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSETGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
     gene            complement(71662..>72426)
                     /gene="phtD"
                     /locus_tag="VQ846_000152"
     CDS             complement(71662..>72426)
                     /gene="phtD"
                     /locus_tag="VQ846_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HET3074812.1"
                     /translation="HQDSGNTEAKGAEAIYNRVKAAKKVPLDRVPYNLQYTVEVKNGS
                     LIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNAS
                     DHVQRNKNGQADTNQTEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKESQPAPIQ"
BASE COUNT        21815 a        14637 c        11545 g        24429 t
ORIGIN      
        1 tggaactgac gtagtcagta tcctacacgt acggcaaggc gatattgacg tgatttgaag
       61 agattttcga agagtataat ctttctcgaa tcgctattgc ccctttttta tcccctgaaa
      121 ccgagtagga gctaagacta gatctcttat ctaagtatcg gaatacggct gttcaactaa
      181 attttagcaa gaacttctcc cccttctctc agttcacaga agcgttttat acataccgtc
      241 ctatcagaca atctatttta aagataaccg tgactcagga tcacttctat ctgtcgtttt
      301 ctctttatag tgatagacct cacgggataa gtcgtacatc tttcctcgtg tatacaagat
      361 ataaggacgt taaaaggtta cagtgaaaat agaaaatctt agtacgaatt caattactct
      421 cgtgatttac gcatataggt tacgactacc tttttggact ttagagtttg aggccctctt
      481 atccgctata tacgccttat tatcacgttc ctgttcgtag agccacgatt tcgctatccc
      541 ttcttctgac aattcttatt tccagcgtca gacttaaaac ggtctatccc cagaccattt
      601 taatccgcta cctcacgata gtcaggcttg ggaatcgcta tttgactcgc cgagaacttt
      661 gacatcagat atactataac aaattgaaat aagaatcaaa caatttaatt ttgaaattca
      721 aatcaattta taacaatatt ttagaagata tgctgtgcta ttccagattc agtttgctat
      781 acatgcctgt cgtaccacaa aatcccgtgt ctgatgaggc acgggatttt ttagcttctt
      841 tactgagatg gattttcctt tttagttcgt gccaatctca aggcacgatt tggattgaga
      901 cgattaaata gttcttccaa tttcttttca ttccaaacgt ctgcggcgga aacaaaatta
      961 ccatccgcat ctcggaaaga tatcggttta tctcccatac cagtctccta gtctacaaat
     1021 tcaaactcaa atttaccaat gcggaccaaa tccccatctt tagctccacg cgcacgaagg
     1081 gcttcatcaa cccccatacc acgaagctga cgggcaaatt tcatgacaga ttcatcacga
     1141 tcaaagttgg tcatattaaa gagtttcatg agtttttcac cagaaagtac ccatgtcgca
     1201 tcgtcatcac gactaatttc aaaggctttt tcttcttcgt caaatccata gtaagtttct
     1261 tcttccatat cggactcgtc gtagagcaaa aattctggtg tcttgtctaa caattcagct
     1321 gtagcatcta aaagtgttgc cagaccttgc ttggtcaatc cagaaattgg gaagatagct
     1381 ggtaactctt caaattcatc ataattttca gccaattttt tcttaaattc ttcaagattt
     1441 tcctgactct caggcatgtc catcttatta gctacaataa tctgtggacg ctccatgagg
     1501 cgaagattgt aagactccag ctctttattg atagctaagt aatcctcata tggatcacgg
     1561 ccttcgctag ctgacatatc aatgatgtga aggataacac gtgtacgctc gatgtgacgg
     1621 aggaactgag ttcccaaacc aacaccttga ctagcccctt caatcaaacc tggcaagtcg
     1681 gctactgcaa aggattcacc tgattgggtg cgaaccatac ctaaatttgg tacaatagtg
     1741 gtaaagtggt aggcaccaat tttaggctta gctgaggtaa taacacttaa aagtgttgac
     1801 ttccctacag atgggaatcc tactaaaccg acatctgcca agatttttag ttccaattgt
     1861 aactcacgtt cctgacctgg ttctccattt tcagagattt ccggtgcagg attttttggt
     1921 gtcgcgaaac gaatatttcc acgtccacca cgaccaccgt gggcaacgat aaattcttgc
     1981 ccatgttcaa tcaaatctgt taaaaccttg ccagtctccg catcacgaac agtcgtacct
     2041 tgtggtactc gaactctaag gtcctcagca ccacgaccat gcatcccttt ggtcatccct
     2101 ttttcaccag aatcagcctt gaaatgacga ttgtagcgaa aatccatcaa ggtacgtagt
     2161 ccttcgtcta caacgaagac cacattgcct ccacgaccac catcaccacc ccaagggcct
     2221 ccattaggga catatttttc acgacgaaag gcaaccatac catcgccacc attaccagcc
     2281 ttgaccttaa tcttagctgt atctaaaaac atactcatta tttcttctca ctttaaaaaa
     2341 gggctgggaa atcccagtca ctaaattttc ttgaatctat tttatagatt actgagggca
     2401 ccaattgcag ttgcaaaaat tcccaataaa cttgctacta gcatgataat cacgataaac
     2461 aaggttattt tctcaaacat agttttttta cgatttccat tatctccaaa tgccattttt
     2521 ctctccttcg ttacattcta ttttctatta tcttagcatg aattgagcta gttttcaata
     2581 gtcagttgct actggtgcga taatccataa aataatgtaa gctacaattc cagctccgta
     2641 acagcaagta agaacacccc aaatgacttg aacgatagtc ggatccatat ccagataatg
     2701 ggccacccca gcacaaacac ccgcaatctt tttatcacga ccgcttctca ttaattgttt
     2761 ttttcatagc tgttcctctt tctattcttc atggtctttc caatagtctc tcatactgat
     2821 caactgtgtt tttgaagcct tcaacatgcg tctagatcct tttacaggta cagcaaccac
     2881 ctgttgcgga agatacaaaa ctgtcttaaa gatacgctga ctgaccgtat catctttagc
     2941 caaatctttc tggaagttat ttgaaataat ggcatccaga taaaactcat gcactcgttt
     3001 cccaaagttc tcagctgaaa tctcatacaa tttctctgat aaggtatgct cattcatgtc
     3061 tggtgttgca atcagggctt ccgaaatagc accagccaaa tcatgttctc catagtacaa
     3121 ggttccaaac attttatcac tgatgaggtt gttcaaataa ggatttccgt gagcaatgac
     3181 aggtgttcca ctggctaagc tttccaagta ggtcaaacct tgcgtttcgc ttgtcgatgc
     3241 cgaaatgaag aaatccgccg ctttatagta aagagccgtc tcactaggag caatcatccc
     3301 tgtaaagatg actgagtctt gaatctctag gttctgggct tgctctttga ggtcattcag
     3361 ataagggcca tccccagcta ctaccagttt aaccttgtct tcctctttca gaacatcagc
     3421 aaaggctact aaaactgctt gaatattttt ttcataggag attctcgaaa gactaagcaa
     3481 cgtcttttca ccatcttgaa tccctagttt actacgcagt tctttcaaat tttcctgctt
     3541 gatttccgga cgctcaaact tggctaattc aattccagta ggaatgaccc gtttttcaac
     3601 cttgacctta taatcagata gcaagtcacg gacaatctca ctagggcaaa taaccccatc
     3661 cacatcatgc aggaaacctc taaccagata cttgaccata ctcggccgga tcaacatccc
     3721 cttagcaata taatggacat agtcttcata ctgggtgtga taggtatgga tgactggaat
     3781 tttcaattca cgcgcaatcc aaatccccaa caggccaaga gaaaattctg tctgagtatg
     3841 gataatatct agctgatact gtttagcaat ttcaagtgcc ttgctaaaac ctcggtaggc
     3901 aaagcgacga tccttaaaag caaagaaagg aacacttgga atgcggataa tttgccaatc
     3961 ttcgtagcga ttgacatcct tatctgtcgt cgtaaagata aaaacagcat gtccctgctt
     4021 ttcaagttct gttttcaagg ttcgaatact ggtcgcaaca ccagaaacct gaggaaaata
     4081 ggtatctgta aataaaccaa ttcgcataga ttacctcact ttttactttc tccctaaagc
     4141 ggcttgtttc tcataaaagt ccaaccagat ttgtaacaga tgctcttcag aatactctct
     4201 ggaaatattc ttagcctttt ctttgagatc ttttaaggta gcaggatttg cttgatattc
     4261 caaaatagcc tctttcatct cttctctacc cgctgtcgcc cgataatttc cctccaaaat
     4321 caccttatag agatctaaat cacgcaacat aataggagcc tcacaactcg cagcttctaa
     4381 aatagtcata ggaaagagct cattgtaact aggcaacaag aaaagatccg ctagagcata
     4441 caattcgcgc atccgctctg gcgatacaat gcctggaaaa atcaaatttt tagggggatt
     4501 ttccataatt ttcttatagt gttcataacc atctgtcata ccaccaaaag agaagccacc
     4561 agcccagata aaggtaatct gaggcaattc ctcagccaga cggataaagt catcaatccc
     4621 tttacgtttc tgaacttgcc cagcacctac tacgataaac tgattgtcac taagaccaag
     4681 atctgtgcgc agtctaacta cctcttcttg tggtagagga tgccattttt ccttgttgac
     4741 aaagttagga atataggtca ctttttcacg tggaatacca gctgctacca aatcctcaat
     4801 aaacatagga ttgaccacaa ccaagtgctc catccggttg taaaaagaaa atacatagcg
     4861 tttcacaatt ccctttaaga aaaatggaat tttcaaactt ccctcaagtg tagctggcaa
     4921 gaaatgcaca tagccaatct ttctccctga gcgtttcttt tggaaggttg ataaataata
     4981 gggaaaatca atcgtatgaa agtgagtcac atctgcctcg attggaagat tttctgtaac
     5041 aatcaattgg tccttggcag cacggtgaag aagacgaact aattcacggt aagcacctga
     5101 aactccctgt cctgctactt tctcacttga actcaacata ttgatgcgta atttcttttt
     5161 ctccataact actattatat cattttcttt gaataaaatc agcaaaagaa aggagagact
     5221 agaggaaaag aggggaaatt tcctcgcttt tccaacagtc tctcacatgc ttttatatgt
     5281 ttacttaatg cctagggcaa tgcgggcata gcgactcatt ttttcaactg tccaggctgg
     5341 ataccagact aatttaacct cagtatccgt tacttctgga acctctatca tagcatcata
     5401 aatctggtct gtcaaaaggt ctgctaaggg acaacccata gttgtcaaag tcatgtcaat
     5461 ctctgtttgc cctgtgtcac cgtcaaaacg aatctcatag atcaaaccaa gattgacaat
     5521 atcgattccc aactcagggt cgatgacttc ttccaaggct gttaaaatcc gtgttttgat
     5581 gttttcaatt tgctcttctg tataagccat attttcctca ctcttagtct tcaataaaat
     5641 cacgaagcgg tttgctacga cttggttggc gtagttttct caaagccttt gcttcaatct
     5701 gacggatacg ctcacgagtt acgttaaaga ctttccccac atcttcaagt gtgcgcattt
     5761 ttccatcatc tagtccaaaa cgtagacgca gaacattttc ttcacggtct gtaagagtat
     5821 ctaagatttc atccaattgc tcacgcaaga cgatacgagt cgtataatcc actggatttt
     5881 caatcacttc atcttcgata aagtctccga ggtggctatc gtcctcttca ccgataggag
     5941 tttcaagaga tactggttct tgggcaatct tcaagatttc acgaacctta tcaggtgtca
     6001 tatccattcg ttcagcaatc tgttctggtg tcggatcttg ccccaattct tgaaggagat
     6061 tccgctgttc acgaaccaat ttattgatag tttcaaccat gtgaactggg atacggatgg
     6121 tacgagcttg gtccgcaata gcacgagtga tagcctgacg aatccaccaa gttgcataag
     6181 ttgaaaactt gaacccttta gaatagtcaa acttgtcaac cgccttcatc aagcccatat
     6241 ttccttcttg aatcaagtca aggaactgca taccacgacc gacatagcgt ttggcaatgg
     6301 aaacaaccaa acgaagattg gcttccgcaa gacgttgttt ggcttcgata tcaccagctt
     6361 caacagccag tgccaactct ttctcctctt cattggtcaa gagaggaacg acccctattt
     6421 ctttcaagta catacggaca gggtcattga ccttagcaga agttgaccca atcaagtcct
     6481 catcgctgag ttctggttct tcttcattgc taagaacacg cgcacttgga tttccttcgt
     6541 tatctgtgat agaaattcct gcatcctgaa tccgttgcaa gagatcttca atcccatcag
     6601 cgtccaaggt aaaaggaata accaaacttg cattgatttc atcatctgtt gctgtccctt
     6661 tttgcttatg attacggata aattctgcta cctgtacgtc aaatgttgtt acttcttttt
     6721 gttttgttgc cattattact ccattcttct cttttgggaa attaaacgtt ccaattcttc
     6781 tagggctgta tctgtatctc ctacatggct agcttcctgc accttctttt tgattctcat
     6841 attgtcctga ttcaagagag ccttgtttcg agtcatctct acttcactaa gttcctgcgg
     6901 cgatatctca gcaggcaaat cctgagctaa aacttggtac caagctcttt caacttcctc
     6961 tgtctgctct gctaaaactt ctggaggaag atttccatac tggccaagca agtcatataa
     7021 gacctgaaat tcaggtgtag caaatgcaaa gtcttctcgc aaacggtaat cgttcaaaac
     7081 aagaggggat tccatcatcc gatagagtag atgggcttct gccctcataa tagccgataa
     7141 ctgcttggtg acaggcatgg tgattggcgt cggtctggaa attctttcca tgcgattctg
     7201 cctttgcacc tgacgactct cattaacaat ctgctcaatc tgggcataat caaaggacgc
     7261 cagactgtca gctaaaatat gaatatagct gttttgagca gcgatggact tttctttaac
     7321 aatcaaggga gctatttttt caataaactc aatctgagcc tgcagatttt cactgttttc
     7381 aggtttgtac tgatgaatgt agaactcaat cggactaata cgagttttcg ttaatagata
     7441 ggccaagtct tctggaccat ttttttgtag atactcatca ggatccaagt tatcaggcat
     7501 gctgacgatt tgcacaggca tatcaccaat ttcatccaat gctttcaata tcgcggcttg
     7561 cccagcctta tctccatcgt aaacaagaac caatttcttg gttaaccttt tcagatgctc
     7621 aacatgctct cgactcaagg ccgttcccat cgacgccaca gcattttcga ttccaacccg
     7681 ataggctgca ataacatcca tgaatccttc catcaggtaa atctcactag cttttccaga
     7741 agattttttt gccctatcca tatgatataa ttcgtaactt ttgttaaaaa ttgcagtcga
     7801 tcggctgttt ttatacttag aagtttgtga atccgttttt tgccagatac gacctgagaa
     7861 ggcaatgatc tttccttggt catttgtcag gggaaacata atgcgattgt gaaaggtgtc
     7921 tacaaattga ttggcatccg agagataaaa caggcctgaa tccagtaaat cctcttcacg
     7981 atactgatca gacaaacgtt gatagagata gtttcgttct ggaggtgcta aaccaatcca
     8041 aaaatgttta agcacttcat ctgtcaaacc ccgctgataa aggtaatttc tggcctcttc
     8101 tcccatagtc gttgtcatga gaatagcatg gtaaaatttg gctgcatctt cgtgcatatc
     8161 ataaagagct tggtgaggtg aggctgactt ctgctcacta taaagcggtt tttcaacctc
     8221 aattccaaca cgctgaccta agatttggac tgcctctata aagggaaccc cttggtactc
     8281 ctcgatgaac ttaaagacat cacctgagcg accacaaccg aaacagtgat aaaactgctt
     8341 atcctctaca acgttgaaag atggtgtttt ttcgccatga aaaggacaga gccctagata
     8401 gttccgtcct gccttttgta aagaaatcac atctcctatg acttccacaa tgttggcatt
     8461 gtttttgatt tcttcaatga cttgtttgtc aaccatacac aatacctcca tgttatcata
     8521 gtatactata tatagtatac tttatttcag aaaaaaagta aactatttca ctcattttcc
     8581 ctactttatt caaagagttg ataataatca gagattttca tttttgcttt ttcttcttgg
     8641 tttaaatctt ggataatccg tccttctttc atgacaatca agcgattgcc gtatttgaga
     8701 gcatcttcca tatgatgagt aatcataagg gctgttagct gatctttctt aacaaattca
     8761 tctgtcaatt ccatcaaagc aacactagtc tttggatcca gggcagcagt atgctcatct
     8821 aacaggagta attcaggtcg cttcaaggtt gccatcaaga gactcaaagc ctgtctttgt
     8881 ccacctgata agaactcaat cggtgtattc aagtgtttct caagaccatt tcctactttt
     8941 tcaatggtta cctgaaattc atccttatag ctagtcaagc gtcgtggtaa caatccacgc
     9001 ttttcaccac gaaacttggc gattaaaaga ttttcagcga ccgtcatacg gggagctgtc
     9061 cccatctttg gatcttggaa gacacgagac aggtacttgg cacgcttctc gggtgaaaac
     9121 ttagtgagat cttcacctaa aatacggata gttccactag ttagtgataa ggtccctgct
     9181 atagtgttaa agagggttga ttttccagca ccatttccgc ccaaaatcgt gataaagtcc
     9241 cgttcaaaaa tttctaagga aacatcattt aaaataatct tttcttcatc aaagccattt
     9301 ttaacgattt tggttgcatt ttttaattct acaattgctg tcatttgctt aacttggctc
     9361 ctttcaagat tgtttgctta aatgttggaa tcatgaggca gactgctaaa atcaaggcac
     9421 tgtataaacg aaggtaactt gtattaaagc caagtgcgat aactgcccac actaaaaatt
     9481 gataagcgat agaacctaca acgatagtaa ccaaacgctc tgccaagctc aaactcttga
     9541 aaataacttc tccaataatc aaacttgcaa gccccacaac gataaccccg atccctcgag
     9601 acacatcggc ataaccttct tgctgagcaa tgagggcacc tgcaagggca atcacaccat
     9661 ttgataagac caagcccatg agctccatgc gtccagtatg aatcccgaaa cttctagcca
     9721 tatcaggatt atcccctgta gcaatatagg cttgtccgag tttagtgtcc aagaaaaaga
     9781 gcatgagagc aataacaata ctcacaaaga tgagacctgt caagagttga ttcaaatccg
     9841 aatcaaaagg caaaacatcc tgaatttgct tggttccaag caggcctaaa ttcgcacgtc
     9901 ccataatcaa gagcatgatt gagtgacaag aagtcatcac caaaatccct gagagcaagg
     9961 ttgggatctt cccttttgta taaagaaggc ctgctgccat tccagccaaa caacctgctc
    10021 ctacagcaac aagtgtcgct aaaaatgggt tcacgccttt ggttatcaaa gtgacagcaa
    10081 cagctccccc aagagggaag gaaccttctg tcgtcatatc tggaaagttt aaaatcctaa
    10141 atgtcataaa gattcccaga cctagaatag cccagacaaa tccttgagaa ataatggaaa
    10201 caatcatatt ttatttaatc ctttctatat tcatcttttt aaaaaatggg aagagtctcc
    10261 tcctccctac cttatttatt cgatgacttg tcctgcttct ttgagaacag actcagggat
    10321 agtaatacct agttcttgtg ctattttttt attgatgact gacttaccag ttgaaaagac
    10381 attgactggg gtatcggctg gttttgcacc tttcaagact tgcacaatca ttttacctgt
    10441 tgccacacca aggtcatgtt ggtcaattac aactgatgcc aaaccaccta cttctaccat
    10501 agctgtcgca ctgggataaa ttggtttctt agaactttga ttgctagaga caaccgttgg
    10561 aaatcctgat gcaatggtgt tatcaattgg aacccaaata gcatctacct tgctagtcat
    10621 aacagtgaca gttgaggcaa tttcatttgt tgaaggaact gcaaatgttt ccactgtcag
    10681 acctgctttt tcagcataag ccttaaattc ttcgacctgt gtttttgaat tgtcttcgct
    10741 acttgagtaa agagctccga ttgttttcac attcggtgtc agagccttga tgagttcaac
    10801 ttgttgttga gctggattgt ggtcagatac ccctgtaatg ttgccacctg gttttttcaa
    10861 atctttaacc aagttagcac caattgggtc tgtaatagcg gccatgataa ccggtaggtc
    10921 ttttgttgca ctagccaacc cttgggctgc tggtgttgcg ataccaacca caaggtcatt
    10981 cccatttgca accaattgtt tactcattgt cgcaacctta ctttggtcac cttctgagtt
    11041 cataaaatca attttaactt gatcatcttt atatccttct tctgcaagtc catcttggat
    11101 ccctttataa atcaaatcaa gggatggatg gctcacaaat tgaagcacac caatcttagt
    11161 tattttcttc tcatccttat tattctgagc ttctgattta ttcattgaag aataaatcaa
    11221 gcttcctgct actaagactg ctaatgcagc aataattcca attaaacgtt tatttttcat
    11281 tctgtttctc ctttttattt cctttaacat atttcactta tctaaacaag cgacgccatc
    11341 gttttctttc catactaacc tccatcaaaa aaatcctcac acaaaaaact tgtgtgagga
    11401 cgtcgatgcg cggtaccacc tcaattatag ggaatttccc tatcgctcag tctcgcaata
    11461 acgagatgca ctgtaaggtg tgcttaccga atttttataa tttcaaattc taaatagcat
    11521 tcagcccaat tttcatcatc gccacttatc tgttttcagc taccacagac tctctaaaaa
    11581 gtttgtatga ttacttttct gaatggttaa attatatcat ttttaaacta cttgtcaata
    11641 gtctattaga atttttttga tattcacttt tagcattcac tcttgaaaat tatcaacctg
    11701 aattacgcaa tcctgtcgca attccgttga tggtgatatg aatcaatcgt tcttgatcac
    11761 tggataattc tccacgacgt tggcgtttaa tcaactctaa ctgaatatag ttgagaatat
    11821 taaagtaagg catacggtaa tccagactag cttttagata tggattgtca gctaagagtt
    11881 cgtcatgtcc ttcaatagcc aagataacgt tcttagtaac ttgccattca tttaaaataa
    11941 tctcatagat ggccttaact tgctcgtctt cacaaagttt agcatattca aaagcaatat
    12001 tcatatttga ttttgacaaa accatatcaa catttgaaag aagcgattgg aagaaaggcc
    12061 aattttggta catatctcgt aagatagcaa tattctctgg atttttattg ataaattcct
    12121 tgaagcttga accaaccccg taccatccag ggaacataac acgactctgt gaccatgaga
    12181 atacccaagg gatggcacgc aaaccaccga tttcagtaat agtcttacga gcggctggac
    12241 gagaaccaat attaaaactt gaaatagcct tgattggact tgactcgaag aaataatcat
    12301 agaaatgctc attaccaaag accaaatcac ggtagatatc gtaactacgg tccactactt
    12361 gatccataat ggcttcataa cgatttgggg tattggtatc gctcttcttc tgagtaatca
    12421 tacggttaat agctgccgat actagcattt caaggttata gtaagcggcg tctttgttac
    12481 cgtatttatt cccaattact tcaccctgct ccgtcaaacg gatacgatcc ttgatagact
    12541 tgagcggttg agatgtaatg gcttcatagg ttggcccacc accacgaccg acagtaccac
    12601 cacgaccatg gaagaaggta accttaacgc caaattcatc tccaatagca gtcaattgtt
    12661 gttgagcctt gtagagggtc caacatgatg acaagtaacc gccatcttta ttactgtcag
    12721 agtagccaag catgatttct tggtagttat ttcgtgagtc aatccatttt ttggcaaggc
    12781 taagagaaag atattttctc attgtttcct ctgaatgatc caagtcttca attgtttcaa
    12841 aaaggggaac aatctgaaca cgcgcccttt ccgtatccac cagtcctact tcttttaaca
    12901 gaatagctaa ttctagcata tcagaaaggt tggttgcatg tgaaatgatg gtctgacgga
    12961 tgacatcatc tcccaactta tctttcaaaa cacgagccgt cttaaaaata gctaattctt
    13021 ttgctaataa ttctgatttt tctgcgtgag tcgcagaaag aattcgggga tcttcttcta
    13081 attctttcaa gagaaggtca cacttttctt cttcgctcaa ctcgctataa cgagaatgaa
    13141 ttcctgctga tttcaagagt tctgccacac aggcttcata gacgctagag tcttgtcgca
    13201 tatcaattga tgctaagtaa aaaccaaata tctctactgc ctgcaataat tccacaaaat
    13261 cacctgaaat caaggactcg cccttatttt ctagtagaga atctcgaatg gcaatcaaat
    13321 ccttgtagaa atcattggcg gtttcataac gagtcccaac ttcttcatcc tcaatcagat
    13381 aggttttagt tgcctgaatt tttgattgaa tatcaaacaa ggcacgacgg taaagctctt
    13441 tttcgcggta aatcgagtta tccttggatt gacgagccat ttctctgact tgcttgctga
    13501 cgttgacaat gctagttgaa agagaaaatt cacgataaag ttggtaaatc tttttatcat
    13561 agtagttcat gatgacttca cactgagtga gtgcagactg cttcaaggtc tttgctgtaa
    13621 caaatggatt tccatcacgg tcaccaccta tccacatacc catggtgatt ggtttagcct
    13681 gttttaaatt cagaccatgc gcttgcgcta agcgcttata ctccgtcgtc aaatgaggta
    13741 cagctttcaa aaaggagctg ttataatatt ccatagcatt cgtgatttcg ttagtcactt
    13801 ttaatttttt ctcacgaatc atgtctgtct gcatgataat ttcgatgtaa cgacgcaaat
    13861 cattgtacca tttatcttta ttgatcaacc ccaacttaac atcacggtat ttacgcaaaa
    13921 gactatgaat atgatttgtt aaatccaaca tacttttgcg ttgcacttgt gttggatggg
    13981 ctgtcaaaac agggacaaca ttcaagtgtt ctaggatctc aacggcattt tccttttctg
    14041 ctaccaattt aatcgttgta gataatttac ctaaatagtc ctgatcaata ttattttgat
    14101 gattgatttc ataagctaaa tccacatcct ctgaaatatt aatcaaaaga ggcaagatag
    14161 agaaatagcg tgaaatatag accatttcat catttgataa gctagtcact agacggttta
    14221 gaccttgata atcttcctgc gttgataatt ctttcaactg tattattttt tcaaaggtct
    14281 ctggggcaag catattttta gtaatatctt ccagtaattc tgttagaatc aagacttctt
    14341 cttgcacaac acttttatta ctataatttt ctaatttttg aagagacata aactatcctt
    14401 tctttatcct acttcaacag aaggttcatt ggttgatttt ccaattctcg gtacaattta
    14461 gcgcgttttt cactggcatc aatatttaag acaaaggcta ctgccactga taagactaga
    14521 agactatttc caccctggga taagaagggg aaagtcactc ctgtagatgg aatcaagccc
    14581 gaaatccctc cgatattgac aaatacctga accaacatca tccctccgac accgagtgca
    14641 accatggcat tgaaaggatt ctccgctcgg ataccgacca agataatccg caaaatcatg
    14701 aaaaacaaga gagctaaaat aagactggca ccaacaaagc caaattcttc aatcacgata
    14761 gaaaagacaa agtctgtatg agcttctggc aaataacctc gtttttcaat cgagtttcca
    14821 agacctagac caaaccaacc gccattgacc atggcaaaat aagaattagc taactggtga
    14881 cctgcatcag cacgatcggc aaaaggatta aaaaaggcac taaagcgctt ggctacatag
    14941 ccaaatactg gaatttttga aaaggtctca acaccgatta ggctgatagt ggtcaagaca
    15001 aagacagaag tggcagatac gagcgccaga atggttgaaa accagcgata agcgattcca
    15061 ctaactgtat acataatcaa ggaaaccaag actaaaatag tcgcatttcc taaatcaggg
    15121 aaaattccca aacttccaat cagaactagg agaacgaatc gccaatcatt aaaagcacgg
    15181 ggaagccatt gattttgagt caaaacttga aaatcataag tagctatttc ttcttgctgt
    15241 ttggagaatc ggtgagctaa ataccaaata ataatgattt ttaagtactc agctggctga
    15301 atagttactc ctgcaaccga aatccaaccg tatgccccgt ttactgaaat accaataaaa
    15361 cgagccaaga acaataaaag catttctatt aatataacta aaatgattag tcgctcattt
    15421 ctcaaaaaat ctagtctcaa tttataaatt aaggcaatca gtatcaaact aacaatccaa
    15481 aagattcctt ggtttcgaac caactgcaag gcgctcttgc cttcttcaat taaaatagca
    15541 ctggtggtcg aatagaccac aatcaagccc aaaatagata aaagcaagta gggaatcaag
    15601 atggaataat ttaataagtg cctcttacta atcttcatag tatcaccact ctactaggat
    15661 acagacaaat ctgttccaaa ttccgtttta ttttctagtt ttgattgcta ttataccatt
    15721 ttttcagaaa gaaaaaaact cttaccttac aatccttcga attttactgt ttttacagaa
    15781 ttaaggcaga atttgaaatc gtaaaaccat tttgaataag agttgcttca atccattgac
    15841 tccgactaat tatgttgctt tcgtgaatgc caaaatcagc cgcaatttgt tcataagtgc
    15901 gctattctcg catgtattga atagttacca taaggagatc gtctaggctt aatttgcttt
    15961 ttcgtccacc ttttgcgcgt ttacgttgat aagctgtttt taacaccgct aacatctctt
    16021 caaaagtcgt gcgctgaaca cctacaagga tcttgaaacg gctatcgctc aattgtttac
    16081 ttgcttcatc attcatagtt ttattgtatt atatttttgt ttcgcaggaa gtctattatt
    16141 cctttcgttc caaggttttt acgcttttag caacaagctt acacactcca cgtgatgcgt
    16201 ctgtagaact caaaaggttc ggggaacctt ttgaggatta attgcgttta tctaacaaac
    16261 tcacacattc aacatggcat gactgatgaa gtcatcataa gaatttcact tgctgctctt
    16321 ttacggtggc agcctgaacg gtcagaagta tcattgtata tcatttgtat catggcatat
    16381 aaagtatcgc tctattttat tgatggtttt aatatcgctc ttatcatggc gaaccattca
    16441 aatctgctcc acaaatgagg aaagtaccat tttggactat tcaattgtca aagaacaaat
    16501 aaaaaaaatc ttcgcatttt cacactctat gaaattgagg agattatttt ctaaagcgaa
    16561 ttttatcagg aaaatccgtc aatcccaata ggtagtctgt actgacatcg taaaagtttg
    16621 agagagttac caatatatct gctgttaacg tatgctcacc ccgttcaatt ttagcataag
    16681 ctgagtctgt aaacgaaagt attttagcta tttgtttttg ggtcaaatcg tgatcttccc
    16741 tcaaatctct caaacgtctg tacatctagc tttctcctga gaaaagtata atatataaaa
    16801 ctaagaaatc attcaaagtg tccaaactgg acattttgaa ctaaaattaa tttattaaac
    16861 atatctcata agcatatcta ttataggtaa gacaatatta agaaagacgg tcaaaatcta
    16921 ttaaccgtct aaagatgtta taagctatta aaaaatcagc agtaatccgg taacaaaagt
    16981 cacataattt tgttaaggaa gggattaatg atgtccagat tactactaac cagcaaattg
    17041 aaaaatggtc aacatgatat ttctaatgtg aatctcttac tttcagaacc taaaagctga
    17101 ataatagcca ccccctactt atcaataaaa ttttccgttg cattaacaga tgtatataga
    17161 ccactccatg gatttcaaag caattaacta gactttattt gtcaataacc acaaaattag
    17221 attagaaaaa tcattaaaaa ttaattggag tcctttgtga tgctttttca ataaaagtaa
    17281 tacttctcaa tattttaact gaactaaaga aatagcataa tggtatcttg attgtattct
    17341 tctaaaaagg attgcccttc acattcaact gtgattgatt aaattccaaa aatatattcc
    17401 aaaggcgctc taaattctct atatcagcta ttttttatca tctatcaact tctatcatac
    17461 ctcttcaata gcgtgctaaa tattagcgat aatatcgtaa tagatacacc aataataaaa
    17521 aaattaaaag tattgttagg atttaaaact actgagaaaa aaacagtacc tagtggcata
    17581 aaaagaatag ctaccgtaaa gatgattcca aatactctgc caagaaactc attatctacg
    17641 tctctttgaa caatagagaa aaattggata ttaaaaatgg aaagaaatag actaaataat
    17701 gctggagtaa aggctaaaat aatgacgttg cggaacatat aatagatcgg cgctgttagc
    17761 attaacatca aacctgaata tgctaaaaag agcattaaac gtttgcttga caaggattta
    17821 ttgataaatc cacttaatat cgcaccaata aagccaccaa tcgcttcggc tgttaaaaag
    17881 gttccatata gcccacttga aatacttcca aacatttgat tactgtatgg cagcaacaaa
    17941 ttataggctg ctaggaaaaa gttaacaagt gctgaaaggg caattatagt gagaatttgt
    18001 ttatgactat atacatacgt aaatcctatt tttaaatcat caaagattcc tcttatcgtc
    18061 atattctctt ttgtatccac ctcctcattt acaggagtaa tgaagaaaat tagtgaggca
    18121 gcaattaaaa acgataatcc atctagtaat aacacaccat gtattccaag taacttatat
    18181 aagaaaattg caatcatagg aattgttact ttaatcacag tacttgttgt ttctagcaat
    18241 gaattaagtt atgatatact gtcctttttt acaatttctt tcgtaaaagc tttgtatgat
    18301 ggccctgaaa aagcactcat aaaagccaaa atgacatttg ttatgactat cgcataaacc
    18361 aaccattttt cttgggatat aaatgaaaga gttatgcaag caataccaca taagatattt
    18421 gtagtaataa taattttttt ccgcttgaaa ctatctgcaa taactccacc aaataaatta
    18481 aatagaacac ctatcatact ttctaatgat tgataaattg caaccactga tagagatgtt
    18541 ggattaagac cagcaagaaa ggtattattg gcgaaatcaa acatgatatc tccgattcga
    18601 gatatcatcc tactagctac aatggataga atattgtgat gttcttctct aatcatttta
    18661 ttctcccgaa tcagtaatca catatttaaa cttttgtgat atgtgcttaa gttcatctaa
    18721 ccttttctca aattcacaac tatttttgca attaaattgg acaaacattg aattttcgac
    18781 tacattttca gatactttat agtaagtatt tggtttaata aatatgtaca ctttttttgc
    18841 ccaagccaat tctttttcat caggtacctc gataacctta ccttgctttc tatataaagc
    18901 aaacgttgct gagatattag caggtctaga aatcacttta gaaaaattta tttttttcaa
    18961 tacttgtgaa ttagtcaaaa tatcccaata ttctttaatt acatcaactc catgactatc
    19021 ctcaattaac tggggaatat ctccccctcc aaatcttttt ccaacttcac aaaagactat
    19081 tttaccagtt gataattcaa ggaaccattc aaaatgaaaa ggggttattt cagaattgat
    19141 agaaaaaaca ttcaaaactt tgcgacattc ttccaatgct ttttctatgt attcaatttt
    19201 ttcactatca ttatataacg aactagtcct tacaagataa tatccactag aacttaaggt
    19261 attgaacaat agttcctcat attcgtgaac ataaaatcgc ttaatctcac ttccaattga
    19321 ataaccatca catgttatca tgttatcgat atcaatgtat tcctctataa tatagttttt
    19381 ttcaaaatac actgaatcaa ttttctcgtt agggtaaact ttttttattc cattacttcc
    19441 tgcttcattt ctaggtttta aaattgcagc agaagattta caattattcc aaaaaacacg
    19501 taaatcattt tctgattcta aaagcctaaa atacggttga tttactactc caactaaaaa
    19561 tgaacgcatg aagtatttgt ctttaaataa taaattagac acagagtgag tatttttttc
    19621 tacaaaatga tcattcaaaa aacctactat atccatgtct tcctcactca aggttgtaat
    19681 actctcaact tctgtatttt gacttatcct tctaatttct aagatgatat ttggtagaat
    19741 aaaatcttct aaaaaaatga ccttaggtag attatttcct tcagctagat aatcttcata
    19801 tttttttctt tgaacctcag gtgcaataat tactacattt ctgttataca gatattttag
    19861 tttaacaacc cgtgcagaat atctaaataa tactatatgc atgttaatcc caatcccaat
    19921 cctttcaatt attagtcatc atatccattt ggatgtttct gttgccattt ccacgtatct
    19981 tggcacattt tcataatatc ataccttggc ttccatccca gtagctcttc tgcttttgaa
    20041 atatctgcaa ttgaaacgtc catatctcct gctctccttg aagttatctt atatggaata
    20101 tacgagccta tgacactctc taagctgtgt aggatttcaa atacactaaa accaacacca
    20161 cttcctaaat tgattacttc atataaacct tcatctgtat taaaaatata ctctaatgct
    20221 tttctgtgtc cgtctgctaa atcgttaacg tgaacatagt cccttataca agtcccatca
    20281 ggagtactgt aatcaactcc gaatatattt aaatatggta attttccaat tgcaacttgt
    20341 gtaatgtaag gaacaagatt attcggaatt ccatttggat cctctcctaa atcaccactt
    20401 tcatgtgcac ccaatggatt aaaatacctc agacaaacaa ttttccaatt tgaattcgaa
    20461 ataaacaaat catttaaaag tacttctata tactgttttg ttctcccata cgggtttgaa
    20521 gcttttaatg gattagtctc atagaatggc atgataggag tactttcgta tacagtagct
    20581 gatgaactaa acacaaaatt aaatacctta tattttttca ttagttttaa aatatttata
    20641 gttgataata aattattttc ataatacttc aatggttcaa ccacggattc tccaacagct
    20701 ttataccctg caaaatgaat tactccatca aaattatgtc tcttaaaaac attttccaat
    20761 ttttcttcat tttttaaatc aattttataa aaatgaagat ttctactctt cttggatatc
    20821 tgcttaatag actcaataac ttttttctta gagttactca aatcatcaat gattgttact
    20881 tcataattat tatctaacaa gtccaaaacc gtatgtgatc ctatgtatcc tgctccacct
    20941 gttattaata atgatttcat gctaatttac ctccatattc tttatgccat taatttgaaa
    21001 tttatcttgg ctaaaaatta ccctatttga ttttgcttca cttccaagaa agttaccgat
    21061 agaaatttct ggaaattcaa aaatctttct agactctaat ttatatttgt ctatgtctaa
    21121 caaagtatat tttccatatt gattaaccag attggaatta ttaaacaaga tcaagggata
    21181 gttatactcc cttggtaatt ctgctgtaat gattgaattt tgatttttaa attcgaagtt
    21241 aaattcacaa atcccttccc taaaaaaatg aagctctaca tcatcaaata catagttaca
    21301 gtccaattcc ttatgaaaac ctagataaaa tcctcttatt aacgaatagg gatttgaaac
    21361 atatgataca accggaaaat taaaataatc ttctccaata caaagaggta tcttaaagat
    21421 tatttctgta tataagattt cattattttc attaatttct gaatacatat cagaaaaggt
    21481 aagatagaat gattctactg attgtagttg tcgatacaat tcttgatcta gagacttaac
    21541 atctgggtat aatacactag catcaactgc tttatttagt ttaacttcta gagttatatt
    21601 attaccttta aatttatttg gtaatcttat catagttacc ctcccacttt aatttttaat
    21661 attttagaaa ctattttgtc aaactcttca atagaaagtg aagtatcaat aaatggaata
    21721 tctgtaaaat atctaacctc atctaattta tgattaattt ttatatgtaa attctctccg
    21781 attaagtatc tatatacttc atttgttatt ccatacctat cactcaagat atctctaggg
    21841 tacttttgaa ttaactttgg atatttttta gtaatattgt caatttcctt cattaaaaag
    21901 tagatataat catttctcaa taaatgattt actttcgtta tatcaagttt ggtaattact
    21961 gagtataaag cgaatttttt tagtattttt ttcactgtgt gccctttata atttcctgaa
    22021 atttcaatat ttttagagga taattgaata agacaatcca acaaggaaac tactcgaata
    22081 ttttcttggc tatcaataaa ttctagaaac aagctttcta tattgaaatt agaggtattt
    22141 ccaaaaaatt taaatttttc tggaatctct ccatgaagaa agtaatccaa aagtttcagg
    22201 tagaatttat taatatttat ctcaaaatat ttcttagact gaatcttaaa ttccgtacag
    22261 tcaattatag gaataaactg tcctccattt ttcacaaaaa ttttaaaata atttccaact
    22321 tttgagtttt caataaaaaa tggaatctct tttgtaataa tataatagtc tctatcaatt
    22381 aaattcctaa ttaggtgttc gtttgaagta ataacaacaa aatctaatgt tttagacgaa
    22441 ataacttcta tttctcttag aagactagtt aaaaaattca ttgctaatga atcttgttta
    22501 ttaactaata agaaattata agtttgatta tatatttccc aatacggatt tatatattct
    22561 aaatccaaga ttcttgtcca aattgtttcc tggggataga aatatatccc ttccttaagg
    22621 ctttcatagt catgagccat taacgctgaa cctatgaacc aatcacctct caaaaaacca
    22681 ctatctttca gtgtaacaat atcatatttc ttaataattt cagaaaacat tttctatcct
    22741 ttcactctat ataagcacca aatcatttct ggagagaaag tttcacaaat aataatatca
    22801 tcattattaa ttatcgtttc ttggcttact attgaaaaac gataaccttc ttccagaaat
    22861 tttttatttg ccataacttt actatgggta aaatctatcc tcagatccat actttttttc
    22921 tttaaatagt ttaacatcaa aatctcagtt gcaggttgga taatcgccct attaatttta
    22981 taattacaat tctcgattag aaccattccc ttagtaaaag agtgaaaaga atccacaatt
    23041 tcaatccgct tatgatattc aaagttgaaa tctttccaac tataattttt aatactactc
    23101 tcgaaagcta caggtaataa tatatattgc ccttttaatt tatcaaagca attaactata
    23161 ctatcaaaac tatcatacag tacaatcaca tctactttta attttatacg atttattgca
    23221 taaaaacaat ctgttgcagt tggtcctaat gtatttatta tcatctttcc cccaagctca
    23281 aaatacaatg ttcaaagtct aatccttgat tttccaaaga tactaaatcc ctcatctcta
    23341 aataaatatt ctcagaagtt tttacatttt ggaaaaaata ttcacttgaa tccaaaattt
    23401 gtattttgtg ttcttttaaa acagcaaata ttctttcaaa atctaggtgt tttatctttt
    23461 tatcgatatg atttaatgtt gcaatctcct tccaatactt ttctagtttt aattccaaat
    23521 aattattctt tgaaattaaa tattttgatg aataataaca aaattcctga atcatagagc
    23581 gagtctgctc cacaatattg gtaggtgagt ctaaagcttc tattatttga ttttttatat
    23641 gcagattatc tactaaaaga ttatctacac ttctaatact attaataatt ccacgataac
    23701 gacttcgatt tattctttct ttcaaacaaa aaatcaaaga aattttatgt acaaagtgaa
    23761 accttccctc taaagtcttt tttatactaa ttaattgctg aatactgtct actataacta
    23821 gaatatcaat atccgattct aatttcccat ccttgattct tggattacct cttgccaagg
    23881 aaccagtaat aacgagtgaa atttttccta cctcgcattt tttaaaaaat gaaattagta
    23941 tatctaattc tttagacagc aagtcattcc tataagttat catactttcc tattaccgta
    24001 taatcttttc cactatcagt tactaagcaa tccctatatt gctgtaataa ataatttatt
    24061 tctttcactt catcatgaaa gactgaaaaa ttagcatatt ctaaatcttt taccatatat
    24121 tctactctaa agtgtatgtc atcaaaatct tgatcattga tctgttctaa tttggagttg
    24181 catactaaac tcatccaacc atcattacat acgattgtat tcgctacctc tagagacttt
    24241 ggtgaaaaat catactgtaa agggttgaat agcttaatag agtaaagcaa atacgctccc
    24301 cacgagttat aaattaactt gaattttgtt ggttccgtac tcttttcaaa aaagtatatt
    24361 ttttttgaaa ttaattttaa taattccatt aactcctttt tattatagtt tacaccttca
    24421 tcaatatgta aaaaaattga tagttgacca ctattacttc ctaacttacc tcctgtgaaa
    24481 ggagcctcta tatagtatcc ccctttaatt tcaacagttt ttttaaattt ttcaacgcaa
    24541 tcaatcctgg aggttgtaaa atcaattact atggttttac gaagtgattg tatatttaat
    24601 aaaaactcat taatgaaatt atccgtttca atatgattac taaaacaagt gattataaaa
    24661 tcaacctgat ttatagcatc aggtaattca tcttctctaa taaatattat tccatttatt
    24721 gttgaattta gatgcctact atataggtat atattatatt ttttcgactg tttcaggatt
    24781 tttaatatac cccgagtcat tataccatac cctataatta atactttttt atgtttcaaa
    24841 gtctcaccac tctctaaaat tttacgattt aacttaaata tgttttagct agtaagtata
    24901 gtctttagat actctaaaga cttaggtgta cacctaagtg atttaaccac caacaataat
    24961 gataatgtcc atcactagca tcaaaattgg gaaaaatttt gaaattttct tcatatttag
    25021 acttcctttc aatttttatt tattgtacaa taaataaaac taagttaact ttttgttggg
    25081 aaaatgggaa aaactatgaa atcaaaactt ggcatcacac ttagaaaagt tcgtaaaggc
    25141 aaacaaatca gtctatgctc tgttgctgat gagcatcttt caaaatcgca aatatcccga
    25201 tttgaacgtg gagagtcaga gatatcatgt atccgactga tcaatatttt agacaaatta
    25261 cacattactt tggatgagtt tcttatttta cacgatgaag actatacaaa aactgagtca
    25321 tttgccaact taatacagta tatccgtaaa caatattcat tacaaaacat caataatata
    25381 caatctctac tttcggattc ctcaaattac actttagatt cttttgaaaa aacaatggta
    25441 aaatccattt tacacacaat ggattcaaga atcattccat cagatgatga attacttcaa
    25501 ctagcagatt atctctttaa agttgaaaaa tggggttatt atgagattat tttattaggt
    25561 aactgtgtac gaacaattaa ctacaactcc tacttcttac ttaactaaag aaatgttaaa
    25621 taattatatt tactcatctc taaataaaac aaataagcgg attgtcactc agctagctat
    25681 aaattgtttc attctcagca tagataaaga agaattttct aattgctctt atttaattaa
    25741 tgagataaag ggattgttag agaatgaatt aaacttttat gaacagacag tttttctcta
    25801 tgcaaccggt tactatgaat ttaaacgtca attaagtagt ggtattgaaa caatgaaaca
    25861 agctatacaa gttctcgata ttttaggaga atacaagtta aaattacact atactgacca
    25921 ttttaataaa ctagtaaata aaaaataaat gaaattattc tctatctttt aactaattag
    25981 tcgtaaataa tattattcta gtaaggcatt ctcgtgaaat tttagaactg taaatttatt
    26041 ggtattaata attttcacaa acaaatccaa tcttctagct atctcctcat atttattaat
    26101 ttcattacca agttcctgtt gtaattttaa aatatccgta ttgagtcgat ctaacattat
    26161 tgattccgtc atgttcattt ggtcaaaatc atacttagct aattttctag tctcatgatc
    26221 ttcactcttc aaattgataa ggacttccgc catcttgttt aaggtggcga ttttttcttg
    26281 ggtttcttct agctgcatat caatctctgc aatttctaat acaagctcat ctttgatttc
    26341 ttggtattgt ttgttcgtgt tagatagttc aatcataaga ttaattttag aaatcttctc
    26401 ttgaagactt tttaaagttg gtcgtctata ggggagtttt tggctatcgt tgctcaattg
    26461 tctgattaag gtcctaccct tgatgaaaca attattatcc atgttttctt tattatagac
    26521 aaagtaagaa gacgtttctc gaatgtagac tttatatttt ttctgattgt cttcttccat
    26581 agtatccaat tgataattgg gaatgaaaat aagaccactc tgcttaatac caaaagacac
    26641 tttgatataa attccctcat caactagctt ctctatttgg ttctctgcaa gttccacttc
    26701 aaattcacga acagcatctc tcttttcctt aaatatctca aaggcttcct caatctcttc
    26761 agtggatact ttatccttat ctcgttcttc ttggaaagca tggtactgtt cctataaatt
    26821 ctctaatcct tctgaagcaa cgacttcctt atttttaaaa taatcttgaa aaaatttgac
    26881 atcatataat ttcttatcac ttatttttta tgacccaaac ttatcttttg attatcttct
    26941 tccaggataa aagttacatt tttttgtttt aagtcaatgg ttagattcaa ttcttttgct
    27001 tttgttatta actcttctaa agaattgaca cggttcaaca aaaattctaa atgactttca
    27061 atctcttgtt tggcaaaata tgttttaaaa aagtcttcat catataaatc tcgtttacta
    27121 agttggcgac ctcgaattgg ttttatcatc gttctatctg tcatcaaaaa acggctatgc
    27181 ttttgactaa aatcaatctg aacatgcaac tgctttgctt tctctaaaaa atcatcaaac
    27241 gatttagatt gctgaagcaa aaaataaaga cgttgtttca attcaaattt atgactagat
    27301 tccttatatt ttttataatc tcgataggaa taacgtttct caataatttt tgcaccagcc
    27361 attttagaaa tacggtctga aatcatacgg aggtttcgtt ccaaggcata gttccatatc
    27421 aactttttat ctgaattgcg gtcaatagcg ttaattagga tgtgattatg aacatgatct
    27481 ttgtctgtgt gagttgctac gataaactta aaacgacctc ctgtcaattc catcatggtc
    27541 tcataaccaa tgcggttaat ttcttcaggt gtcagattat cttcaggaga aaatgattgg
    27601 atgagatgat gggcatgaat agtttgttgg tgtttttctt gtcggtcatt tctggactcg
    27661 tacaacttgt cgttattagt aaagttaaca ttgtacattt ctaccatttc tgcatggcta
    27721 ggaaaatcta agtaattact catgccaaaa tcagatacta attttagatt atctgtttta
    27781 tctggattga ggatatattt aatcaaacgc ctacgatatt tttttccgtg cgtcgcaaag
    27841 tatttagtaa ctaccatgaa actccttcag tcttttcacc tccacttgaa attctttgtc
    27901 cacttcctta attaactctc cgactccttt actcaattct tgtaattggt cctgagaaat
    27961 cagatggctt tgattaatcg cacgcgcaat ttgattgatg ttatttccaa tcctcctcaa
    28021 ttcaaacact aactgttcat aagtatcagt gttaatcgtg acaaatgaca tattgggatt
    28081 aagcaaaact tttctggcgt agactgagaa attttgacag tgactcttag taatacgatc
    28141 atttaattaa ttcaattctg aatcagttag aaatattttt ttagattagt acgataacga
    28201 taaaccatag cagtctccta tcccatatat ttttcacaga attcacgact gagtggctga
    28261 gcttgattga cttctacaat taattcttga acacaagtca ataagattga gacgtgctct
    28321 tgagtcactt gatgattctc tcttgcgata atcaaaacca aatgcacatc tcgactgatc
    28381 tgttcaaact tttgagactg ccaaagggaa aaccattctt tggcagctaa ctgtttgtca
    28441 tcagtcatca aaattttatc cctgacgaat ggtgaaaagt gacgataatt atcaacctcc
    28501 atcatttttt caatctgttt tagctcatct gcggttagat ttacctcttt tcgaatgtca
    28561 cggtactgtt cagacatctc ttttcctcac tttcatcctt tctcgggtaa accccgaccc
    28621 cgtgtgtcat acgggaaatt tcttaaagtc cttgttcggt ctttgcgagc tcaggcattg
    28681 tggtcacaat acccaaaaaa tcatatcgcc agctaaccaa aaccttccag ttttgacagc
    28741 taaccataag ataacttggt ggctacgccc ccaaactccc aggtaaaatt cttagttgga
    28801 tttttaaaag ttgagaaaag tatagcagaa gaaagttggg gaagttatca caaatcaagt
    28861 agggactaat ctctaacacc actgcatgta ccgtcctata tttatgatca gctcccatcg
    28921 tttacatttt taggtcactg tcttttcata ccaggaatta gaattgacaa atttaattag
    28981 agaggtattc atttaattgt tcacttcttc gctccatttc cattacagaa atttcttcaa
    29041 tactatgtgt tcagctgact cccatgattc gttgtactac gtcctaaacg ctcacggaat
    29101 aagccgtaca tctttcctag tttactctcg taatttacgt atataggtta cgacacctat
    29161 ttggatttta gagttcctca tccgctatat atggcttcaa gtcacgattc tgtttgtaga
    29221 accttgattt cgctaattcc ttcctatccc ccctacatca cgatattcag gcttaggagt
    29281 cgctattggt ttcatctgca gcaaatatcc acagaggact tggacctcag atgtacacta
    29341 tgcctgtcga acaaagaaaa aagtaggcta aatttctaac ctactttttc tgactctact
    29401 ctgataaagc cgtctcatat atagacattt tatgcttgaa ggaatctttt cctccctcta
    29461 attgattgat gatatgattt tttatatatt tttcactaac atatccctgt gattcaatcc
    29521 atccgttaac tccatctgac tccataataa caacttgatc tgtcatagaa tttgttacaa
    29581 ttgtagcatt atgtgttgct aaaataattt gacgttgagc tttcacatct ctaaactgct
    29641 gaactaaatg cctgtaaata taacgattgt ctagattgtc ttcaggctga tcaataatca
    29701 atggtctgaa gtctttagaa tagtcactat atgctaataa aaaatcaagc atggctacaa
    29761 ctttttgtcc caaacttaat acaccaactt ctttatagag aatatcaagt tgttgcgaag
    29821 attctttcga gttaatatta aactccaaaa atcgtgtatt ttctttagaa aaaatttttc
    29881 gtttatatgc acctgataag tgagaacctt ttttcaaaat gtcataacta ttgaaatgag
    29941 caatatagtt aactattttt tgattattga aatccttcat tatagtcagt gaatgttgat
    30001 aacttccatc tttcttactt ataatatttt cacttaacca ttgattaacc catgattctt
    30061 gttcataatc atatatacat acaatatgca atttatctgc tgcactaatt tctactccat
    30121 gtaaaatatg aggatgtata tcataaatcg gataattttt cattatgatt gagactgcca
    30181 tttgtaactt tttaatacct ttggtagtat tatgatcagt tactacaact atttctattc
    30241 cattttttat gattgcctct gctaacataa gaaaactaat atattctttt gaactagaaa
    30301 aaacaacctt atcaaaatta ctaaaaaaaa ttgtcttatc aacttttatt ttcttatttt
    30361 caaaaaatat atcatatagt tcatcttcag taatttttct atatttcgtt gcagtccaat
    30421 tagaaaatag cttgtaatca taagaaacag gtgtatgcaa atgaattaaa gtcttgtgaa
    30481 agctaccgaa tttctctttg gaatttgtta gttcttgata aacagacttt atcttgttat
    30541 taaaccctct catttctact ctcctaaaaa tatcaattgt ttatttttca agtaattcat
    30601 taagtctctt ttcccctacc ctcaacatct ccttttctac ctgactccac tctccaaaca
    30661 ataactcatg aagaacatct gctgctgaag ttgtattttc ttttgaatca tatacacaac
    30721 ttctatctcg ttggtaaatt tgaattcttt caaaaatagc tagttcttcc aattgtcgtg
    30781 tattatcaac tagatgattt acaatgaaat catgatgttc ttttggtgtt gcgcgtgctt
    30841 gatttggatt gatagcgtac agttcttcgt atcgaataag agtgctcaga taggacagct
    30901 taggctttgt cgcaatcaag gctaattgta cttcatatcc cttatttttc aagagttgtg
    30961 ccgttttctt tggaacatca actgttcgta aagttccctc tatcaaaaga ttgtatctca
    31021 aactactcaa ttttgttact aaagactcta ccatttttcc tgcaaaatct ttggtatatt
    31081 ctacactgtc tttgccatat tcttgctgca gttctaaata gtgtggatgc tgagaacgaa
    31141 aactatcacc atctatgata acaatatttc cttgaaattc tttctgttta atacgatgaa
    31201 ttgtagtctt accggcacca ctttgacctc caagcaaaat cgctataggt tgtttactgg
    31261 acttttttcc tcttgtcagt gaacgaagat tccttgctaa agcatgtttg aattcactat
    31321 cagtataatc ttggatttcc attaagctac catccgcttt tcagatatct ctaacatacg
    31381 ttcaattcca tccaaatagc cactatatct ctctatttca tcaaaagttt ctactaaata
    31441 gatattcgta tgaatcaagt cagatagatc atcactcatt aaaatccaag gattagattc
    31501 atcatcaatg ctaattccct gactatcttg ataacgatag agtcgagata atagattagc
    31561 acctctttct ttcacaattt caattttcaa agtcaattca taatcttcaa caggattgag
    31621 cattttatct tctcctacaa tatcgacata agatacatta aacttctgac aaatgatgtc
    31681 aattaattcc gtagagactg aactcgttcc attttcataa cgactcaagc tatttcgaga
    31741 aattcctata attcgtgcaa attcgggttg tgttaagtca tgtgttttac gtaaggattt
    31801 tatgttcttt ccaatcatag caaactcctt tatttttata aatccattat agcataaaaa
    31861 agcataatac accaattttg gtgcgttatt tcttgttttt ctatcctaaa ctttacttct
    31921 ataaaagtca cctaaagagc cataaatatt tattcttgta ttaatttttt cgatgcgcaa
    31981 acgatatcta caaatacatc tataacgatt caccttttag gatttttatt gtcaaaattg
    32041 cctttatcta caaagacaaa atgcttataa aatagcttga aatcaaggat tatcaccttt
    32101 ttcgttgtag caacactcca catgtgatta ttaggctatt agcaaagcgt atccaatacc
    32161 tcaataaagt gttgttttta ctgatatttt aactatattg tatagtctac tatttaaatc
    32221 tcaattagga tattttatag aatacccaaa attcaacaat aactaaaagt aagattccat
    32281 tgtatttcct tgttggtctt tcatatcacg aattttattt ttcagaatat taaagttaca
    32341 aatttcatct ctaatatcgt tatccgctct gtagccatta atcatctcag tcagagtttt
    32401 ccctttacta ccattcttta tagtagtatt tactcttttt atactgcttt caaaaggctc
    32461 atatgtttta tacttaatta cttcgtcttt aaaattaaat aaaggtgtat ccggcttagc
    32521 attccataaa ataatcaacc acattatagc attataaagc tgctttgcag tttcattcca
    32581 cttttcaggt tgtaaatttc cactccctgt attgtcatat tcttgtctat tatttacatg
    32641 agaatcacct acaagtggat taaaaagagg tagccctttg tgattaatta gttctaaaaa
    32701 atcccttgcc gcacccatgc aacactgaat aacttccttc ttgccatcaa ttttactctc
    32761 agcgataaat acataatatt catcttcaat agttgctcct gcatcactat gtttttgttg
    32821 atttgctaac agcttaatat gtgctttagg ctaaacttca aaatctctaa caattctttg
    32881 tcttgtttca tgtcctctac aattcattta agttctcctt tacaagttta gtaactagcc
    32941 ttattatact atataagata tgaaatgtac cataatcttc atcaactagt ttttagaaaa
    33001 tcacaaatcc atctggagag atgacaagct gattctccaa ttcttggcaa ttgcttgcta
    33061 gtgctactgc ttctttctct aaaagataat cttctgttga ttggttgaat attgctttga
    33121 ggatccgtcc ttcgtatttc cattccagag ctactaaatc agatttgatt tcttgaaggc
    33181 tatacttgcc atgcgaaata attactgacg cgtatttccg aatcttaatc agcctcttca
    33241 taaagttcag catatcattg tcacttgata cacgttccca aggcatacaa cgacgacaat
    33301 ctggatctgg tcctccagtc aaggctagct cggttccgta ataaatgcac ggtgttcctt
    33361 tttgtaaaaa gagaaaggct aaggctgatt taaccagttg aacatcttca ttggccgtcc
    33421 acaggattcg ctctgtatca tgtgaatcca agagattaaa catgacctct gaaatctgct
    33481 gcttgtaata catagactct ccattgattt catcgatgaa ctggtctgtc ttcttaattc
    33541 ctcgtaagaa atagtccttg atactatcag ataaaggata attcatgatg gcatggaact
    33601 catctccatt tagccaaggc tgagatgtat gccagacttc tcctaggata taaagatcag
    33661 gatttttagc taaaactgcc ttacgaaaat ccttccagaa ctgatggtca atctcattag
    33721 ccacatccaa acgccaagca tcgatattaa actcttcaat ccaataagtc gcaaccttta
    33781 aaagataatt cttgacctct ggattggctg tatttagctt aggcatatag tcctcgaaac
    33841 caaaaacatg atagggtaag tctctcttat taactagttt ttcagttgtc actgggaatt
    33901 gttgaatatg gaaccaatcc ttataagcag actgttcacc atttttgacg acatttttcc
    33961 attgaagaga ttgcgaacca atatgattaa ataccgcatc cagcatgact ttcatgccac
    34021 gatgatgcgc ttgatccacc agttcccgaa aggtctcctt gtctccaaaa tgacggtcaa
    34081 tttcaaagta atctgtcgta ttgtacttgt gattgcttgt agattcaaag atgggacaaa
    34141 gatatagtcc agtaataccc aagtcttgca agtaatccat atgatcaata atcccctgta
    34201 aatcaccacc aaagaaatca tcgctcttag gtgtgacaga tgaatcccag tctaaagtcc
    34261 cttctgggtt taatagagca ttgccattgg caaatctttc aggaaatatc tgataccata
    34321 ccgtatttga aacccagtca ggaaccttgc aggcatcaat cttgaagata aggcaactta
    34381 aatccattcc caattgcatg aagattttct agagaatttt ccacacaccc tttatcgcca
    34441 tacaaaatat tttgaccttc tgtatctctg agctcaaaga gatactggat acgtgcaaag
    34501 tcaactgaca cttcaacctg ccaatggtca aataaggtac cagaagttat cttgaccatt
    34561 tcttttgtat cctgataaaa ctcctccata aagataaaag ggtccccata gtgcaagttg
    34621 atgctttcaa tgtccccttt cttagttcga attcgaatat ggagtttctt atccttataa
    34681 agataggcat actccgactc aggcctatgg taaatagcac tcaattccat caatttgtct
    34741 cctaaagtat tcattcaaat tttacgcaaa cgttttcaaa ctcaatgaat ccagtatact
    34801 atttttgtaa actatttgca aggaatgtcc gttgttaaag gaactatctt ttaaagtata
    34861 caaaaagcct taaaaaaggc tttctctata tactaatcct ctttcggttt tgaaaatact
    34921 tctactcgct gagctaggac tttgatttct acaggtaggt tatctgattt atctccgtca
    34981 acatctgact ccaattcact atctgaagag attttaatat tacgcgcttt gatatactcg
    35041 atattatcat ttgcgacaac atcccctttt aataaatcag gaatgacgga taatttggag
    35101 aatatagagg catctttcag aatcaaaatg ttggcatagc cgtccttgtt ttcttcaaag
    35161 attttcttat cagcgaagta atttgtcaaa aggaccaaaa catggctagc ttcaccaaca
    35221 taatttccat tttctgtctc aaccttaatg ttaaagacct gatctgtcat gacagacttc
    35281 atggtattta cagcataggt taaaataccg aattttgttt tgtcctctat ttcaacattg
    35341 tgaatcgcct caggcagaga accgatacta aagatataac caaaatagtt gtcatttgct
    35401 ttaccgatat caatcttatt gattaaatca aaatccagtt cgtcaattgc gccatcgatg
    35461 tcttgattga tttccaaaag ttttgtaatg aggttacccg taccgcctgg gataatccct
    35521 aacttaggaa tgtagtctct ctcatcaata cctgaaatga cttcattgac agttccatct
    35581 ccaccaaaca caaccactgc atcatactgc tcacgagaag cttcttcagc aaaatgtgtt
    35641 gcatccagcg ctttttcggt aattttggtt tcaacatatt caaagtattc ttttgcttta
    35701 ttctccagct tttctttgta atccaaagcc ttctcgccac cagaagtagg gttgataatt
    35761 accattgctt ttttcattga ttttatcctt aattttaaac agaaatgttt acatttcgtc
    35821 gtatgcaagt aaatgtaatc ctattataca atgaaaatac agaaaagaga aatctgacat
    35881 actggagatt aatacgcttt tattctattt tcccatcgcc taactacatc ctttaagggt
    35941 tcatccaagt aagaataggc cttatccttg atccaatcag gaataccgta agctgcctct
    36001 gctatgctac aagtgattgc tgcgagagta tcactgtcgc caccaagtga gatggcattt
    36061 cttatcgcat cttcgaagtc tctactttca agaaaggcga taatggcttg agggacagtt
    36121 tcctgacatg tttcgttaaa acgatagtta ggacggattt catctaaagt ttgagataga
    36181 ttgtaatcgt attctttttc tatataatcc ttaattcgtc ttttacactc tgtaggattg
    36241 tcgttaattg attgctcgta ctctctacaa taaccaccaa agtaaaaacg acatagaaag
    36301 atagcatcag ctgtagccat agcgcctttg acaccttctg gatgattatg agttacctct
    36361 gcagaaagac tcgtaagtcc tctagatgat ggccacatac cagttttcgc ataaaaacca
    36421 cagtccatga tccaagcaca tggagaaata cgcatagctg atccattccc aaagctatta
    36481 taaggctcac ggttatcgct gtttagccat gcattaaacc gagcaccgta atcagcattc
    36541 ggatacattc tgccatattt cttcatcgcg tcaatgaaat cgtctttttg tccaccattc
    36601 ataattgctt ctgcaacagc acaggtcata accgtgtcat ctgtaaaaaa gcagtccttc
    36661 cgaaataaag gaaagtcctt tgttttgata ttgttccatt cgtaaacaga accgacaata
    36721 tctccaataa ttgctccaag catcagattc ctccttgttc attttgatgc tttttatatt
    36781 ggttatctac catatttatt ttagaaaata acatcctgtt ggattttaaa aatttcattt
    36841 ttttcaaaat agggttttac catttctttc cacctagctc tatgaaaatt gattgatttt
    36901 aaaggagata ggccataatt tcccaatgca taaccatcat ttacttcaac aacaagtgtt
    36961 ctgccatcgc gagtaacacc gatatctagt ccataagcta ttggcgcatc tttccaacat
    37021 gatatcgctt catcaattac acttgcatca aattgtgcat gataatcacc tgtatagggt
    37081 cgaacatcta atacgcgacc atctaacaca aaacaacgcc attcagctat gaattctaca
    37141 acctcactaa tccatatagg atagtcgaaa ggtagaccaa tacctattaa atcatgggtt
    37201 ccattaacaa ctcttccagt aaagactttt gaaccagctt taggcttaat aaattttccc
    37261 caattatcag gtatattcac aatctctcct aaaataccag cataaatctt tcgaccataa
    37321 aactctttaa gctcaatagg atagtcatga accggaacgt ttaagcccat catttttagt
    37381 aatgctctag tctccattat ataatctaca actatatctt cacttgttaa ctcttttatt
    37441 tcagaaaaag attgatataa aataacttct tctccttgta agtaggcacc tacttgagca
    37501 ttgtatttat taattgaaac ctcacttggt aatttacttt gtctaatata aacaaccatt
    37561 tcatcactcc tatatcacta gtgttacacc aatttgtaaa aaataatagc aattttgctc
    37621 ttattttttt gagtaaatag cccccataat atcatcgaaa taatcaacgg tatttaggag
    37681 taattcaata acctgggact ttgttagtcg cattcccctt ctatctctag catcttctac
    37741 taaattttca agtttctcta gatttttatc atccaagcta atcattattc tatttttatc
    37801 ggttgccatt ttcatcacct cgagttaatt ctatcacagg tgtaacactt gtgtcaactg
    37861 gcttttataa tacattagtt taaaagtgga gaggattttt aacacagtaa ctttaaatct
    37921 ttggtattaa aaaattttca caatatttat agaaataaaa tctgtctcaa atcagttatc
    37981 aaatctagta taaattatga gcggctactc taatactttc cctctaaaca agaaaaagac
    38041 ttacactcaa gggttttctt ccccccccct tcgttataac gttttgactc ttttactagc
    38101 aaaggtatat actcacaagg aactttggtt gactattgaa tctctccaac ttcttcttta
    38161 acatatcctt ctacatcttc aatctctaca aacattgggt ctaagtgaca caagaaatgc
    38221 caaacttcga tccctttttt tctgtaaaga atcgcttcac cgtcttcact tccgaaaaag
    38281 cttctgtcga tttcatatcc gcggctttct aagaagtctt ttgctttacg atagttcgtt
    38341 tctcttgttt cgacataggc tttaacttca tggttgttaa cgacatatgc atcaattttt
    38401 gaatatcctt cgatcactct atcatttttg agggataaat ttgaaatctc tttccaaata
    38461 atgtttacat tttcctcagg atcgaacata aatttagata aaggaacaat atttccgtta
    38521 aaaataattt ccatataatc cggtatgttt ttaggattaa aatactccac ttcaaaacca
    38581 tcttctgttt ccagagtgta tcccgggatt tgagctacaa aggctttccc atcttctatg
    38641 gaatcaaatg ctactaaatc tttagaataa tcattttggt acaattccaa tataaccatc
    38701 gataatctct ccattttcat tatcaggcta atgtaaataa gcacgtcacc tgaccaattc
    38761 aggctctctg tatcatctca tcatatttcc tacttacttt acgagtccta tacccagaac
    38821 acaccttatc gaccttcggt ctcacctcgt cgcattggct gaacatctac ttttacttcg
    38881 ctgatgcttc aactcgtaca agcagtgata ccgcctcagc gtgatgcgtc agtgggactc
    38941 aaaaagtttg gggaaccttt tgaggattaa ctacgtttct ctaataaact tacacattca
    39001 acttgttcat cattgtccaa atctatgttg agattttctt ctataattgg tagcttaaaa
    39061 gtaatggatt ttagccattg tccgttagat tgtttttctt cataaacttg aatttcagaa
    39121 atcaaagctg aaattaactg cctacgctct acatcattca tgactttata gagcttatca
    39181 aaatagatca gaaccttata tatgttatct cctgtaagct tttcagcttc aatagtctgt
    39241 ttctttgctt tcgcatcaat tagtgatgat tctaattcat ctattttgtc atacatacga
    39301 tatagtctat catctaaatc ctgtttcctt ctcttataat gcttatcttc aacatctaaa
    39361 ttatctattt cctcaattag cttaaacttt gtagaatgac tctttctcaa ttccttttgg
    39421 taattatcta tttctttttc tatttcagag gtatccacct tcatgttgat tttttcttgc
    39481 atcatagaag caaatttcgg attacttact atcttgacaa tcacctctgc aacagcatca
    39541 tctaacaatt cttctctaat ttgcttactg aatgtacact tatgacctct tatcatctgc
    39601 ctatggttac aaccatagta ataaaaatct ttatactttg tgccatcttt ctttttcttg
    39661 atacacttgt tcccaaacat tcccactcca catatcgggc attttacaat tccagaaagc
    39721 aagtgtgtgc gtgtatcttt tcctttattc acatgctcat atttctttgc ttgagatttt
    39781 agcttaacct gagcagctta ccaaacttca tcggaaacta tagcttcatg tatcccttca
    39841 gatattagat attcatcttg ttcaacctgc ttatattcat ttcttgtacc atgaactttt
    39901 tctaaagttc ttcttccaaa tgctattttc ccattatata caggattctt taatatcttt
    39961 cttataagac ctgcatcaaa caaaggattc ttaccattct gtcttgggat ttttctaatt
    40021 ccatgattct ctaagtattt agatatccca ttggctccta tcgtagtatt tacatactgg
    40081 tcgaaaatcg ttcttattgc aactgcctct tcctcattta taaacagctt gccgtcttca
    40141 agtttatatc catacggagc aaagccacca ttccattttc cttcccttgc tttttgaatg
    40201 caaccttcca ttgtttgaat acggatgttt tctctttcta tttcagccac agctgataaa
    40261 acagaaatca ttagtttccc agcatcttta gatgaatcaa tgccatcttc aacgcagata
    40321 agattaactc cataatcctg cattatctga agtgtagaaa gaacatcagc ggcatttctt
    40381 gcaaatcttg ataacttaaa cacaagaaca aaagatactc catcttttcc agattttata
    40441 tcttccatca ttcgattgaa ctgtattcta ccttcaatag acttgccaga cttcccggca
    40501 tcttcatact ctccaacaat ttcataatcg ttgtaaatag caaaagcttt cattcgtgat
    40561 ttttgtgcct ctaacgaata cccctctatc tgtattgacg tagatactcg tgtatagagg
    40621 tatactttta ttttttcttt tgacatagta ttaacctcaa tataattttt ctatatcata
    40681 tataattttt ttaatttaag tttggactat catttcaagt atattataac acttttatta
    40741 gtccgtctca atttgtgttt ttgccatgtc aaaactattt ttcatctctt gattttttgc
    40801 tggcgttgga tcgggtagat tatctaaatc taaagcacca gcatattttg caatcagatt
    40861 tgctattaaa tcagccaatc cattccagtc attgtccaat atatacctcc tctaaagttt
    40921 tatatctaat aattatttgt ttaattaagt tttttgacat tgacaagtgc tttggattag
    40981 caacatagga atctcacctc cgcctctatt ccggatgagc cggcttcaac cttagaagta
    41041 tcattatcct cattttcttc atagcggata gggtatccct ccctatattc aaactcttac
    41101 ttatcgctca ctttcttttt acttagcaga actttttttg ccgaattatt cagccgaaag
    41161 atcttgacgg ataggttatt acgctccaaa aataattaac gtcttgtctt ggtctattca
    41221 attgttaagg ttcaaaattt atcgagagtt attaatcttt ttaaaatttg accatcagaa
    41281 aatatttatc ttaatgtaac aaaattctat aaattaccct cttatactta acagtgaaaa
    41341 gaagtctttc ttggtaacca attttgaaat agaatttgct tatataaaaa ggtcaaattc
    41401 ccactgcata agtagcagtg aaaattagac cctcttggta actgtcatct aaaagtcttc
    41461 tagcatttct ttaattttct aatcgccttt aaagtgtcat atttatattt ttttatctct
    41521 ggcatacttt gatacctttt tagacttaaa gtctttaata gtgcctttcc acctcttttt
    41581 atctataaag attctcctac atcataattc atttttttat ttaaaccttt ctgtcttagt
    41641 ttgtctttat cttcttcata ccattttaag attgtcacat agtggttttg ataggtctta
    41701 ccactgcttt ccatgtatct ggatagttta tttatcatta tatctgtgtg tgagtttaat
    41761 ttttctttta gatttttata ttcttctttg cttaacctta catttttgaa ttctccataa
    41821 aaaatggggg tggacttttt atctatctct ccctctctct ctttatctat ctctatatct
    41881 ttccatgtaa ttccaatctg gagtacctct actgtctatc ggtaatttaa ttttgatatc
    41941 tggcaatact gtgctagata tttgatcttt atattcagta ttttttaaag cttgcctaat
    42001 aattgaagtt aaatagaatg ctacttcttt attcaattct ttatttttta attttaaaca
    42061 atgaattttc atatctaggc ttgctttata tttatgataa aagactgctc ctaaaaatga
    42121 aacagatata aaattttcaa aaactctata atttttatca tctatatctt cgtagtaacc
    42181 taagatacca ttgtcaatat ttgtagcact aattctagga gtttttccat cgagtaaata
    42241 tctttttgga atagatgagc ctgttggtac ttgactcgat ttcccctttt tttcggtaat
    42301 aaatatttct ttttattttg ttgtccgata tttttcctac ctgtcctttg taggatgagt
    42361 attttctaga ttttcttgaa taacttttta cttgaagttt tagcttttga actagtcgtt
    42421 gtactttctt tttgtttatt atcagtcctg atctttttaa tattgctgtt attctctata
    42481 tcctattttt cattcatgat attcttttac taattttatc ttaaattctg tgctgtattt
    42541 gccattaaaa aactgacctc ctttagttag ttttttggcc taacttttga gggtcagttc
    42601 aaaatttgcg acttttaaat gaattccgat attcaattat taagagctaa catggtgctt
    42661 gccaatagga atcattagag gcgaattgga aatagggtca cgtataattt ttgcttcaag
    42721 attaaagata tctttaacta gtttatcatt tagtatatct tcaggctttc cctctgcaac
    42781 aagtttacct tctttaattg caaataggta atcagcgtat cttgctgtta gatttatatc
    42841 gtgcaaaatc atgcaaatgg ttgtcttata tttttggttt agatcagtca agaggtctaa
    42901 tagttctatt tgatatgaga tatccaagta agtagttggc tcatctaaaa gtaggatact
    42961 tgtatcttgg gctagggcta gagctatcca tactctttgc ctttgacccc cagaaagttc
    43021 ttcaactagg ttatttgcta gatcttcaac attggcctta accattgatc tgtttattat
    43081 ttcaaggtca tcttttccaa gactcttaaa aggctttctg taggggaaac gaccacggct
    43141 tacaagatca gctactgtta ttgattcagg gattattgga gattgaggta atatagctat
    43201 gtgttttgct aaatcttttt ctttataaga attaattgat ttattatcga gcaatacttc
    43261 tccctctaat ggctttataa gtcgagacaa ggttttaatg agtgttgatt tcccacaacc
    43321 atttgaccca ataataactg atattttttc ttcaggtatt tttatattta tattttccaa
    43381 gattattttt tcatcataac cgcaggtaag attatttgac cacagacctt tcattatata
    43441 ttcctcctgt tcatttttat tagtaagtat attaagtatg gtgaacctaa caagccagtt
    43501 acaacaccta ctggatatct agctggtaaa atattttgag agaatatgtc tgataacaaa
    43561 actagtaaaa ttccaaccaa tccagctaat attgggcttc ttttcttgcc aatatttaag
    43621 gctatgggac cagctaaaaa agatatacaa gctattggtc ctgtaattga agtagaaaaa
    43681 gcagttaaag atacagcgca aaaaattaaa acaagccttg aaagctcggg atttgctcca
    43741 agtccgattg ctatttcttc accaagttca ataatttcta gtcttttatt aaaaaataaa
    43801 actaatatag tagcaataat acttactatt agaacaagag gtatgtcatc taactttgta
    43861 aaagataaag agccactgag ccatctcata acttcttgta attcatatct tgctactttc
    43921 aacaataaaa atgaggtgcc tgctcttgtg acagcttgaa aaccaatgcc taatattatc
    43981 agtcttgctg ctgaaaaacc atctttttta gctagtaaaa ataatattaa agatgatgtt
    44041 agtccacaag ttattgaaat aattccagta gttaaactat ttgtttttaa taccaatatg
    44101 caaaagaccg ctgcaataga tgaagaactt gtgacaccga ttatatcagg acttgcaaga
    44161 ggatttctta acatagtttg aaagataaat cctgccaatc caaaagacca gccagctata
    44221 attcctgcta ataattttgg taatctaatt tccataatcg aaaaactagc tccaggaaca
    44281 gtttcactat ttaagacttt aatcaaagtt gaaaaagaat aactttcatc tccgataagt
    44341 aaaatgaaaa atgatagact gattattatt aataaaaata gtgaggaaaa tagtgttatt
    44401 ctattttttc ttttttgaat acctataatt aaattttgca ttagttatta acccctctat
    44461 ttttcatagt tacataaata agtactggac ccccgattat tgcagtaatt atccctactt
    44521 caatttcacc tggtttacct aacatacggc cgattatatc acatataagc aagagctctg
    44581 cacctataaa agatgaagaa atggtcattg tgcgtatatc tttgcttata aataagccac
    44641 aaaagtgagg aactataaga cctacgaagc caataggtcc accaattgca gtaatacttg
    44701 aacataaaag cacacttgca attattgcaa gtgatcttat cctattaaca ttaactccaa
    44761 gaccaacagc catttcatca cccatagcta aagcgtttaa atctgatgaa ataaatatag
    44821 ctatcaaatg acctaaaatt ataaaaggta gtagtgtaga tatagaagat aatgtagctg
    44881 ctccaaggct acctatttgc caaaatctaa atttgtctaa gacgttatta ttcggtaaaa
    44941 ttaaaaaact tacaaaactg cttaaagcca tactaacaca agttcctgat aaggcaagtt
    45001 ttataggggt aaggcctgct tttccgctta cagcaatcgc gtatacaaaa attgcactta
    45061 ctaagccacc aatgattgca aaacttatat ggcttatgct tgatgaaatt cctaaaaaag
    45121 aaagaccaat tactacacta agacttgctc ctgtgtttat accgagtata cctggatcag
    45181 ctattgggtt tctagtaact gattgcatca atacaccgct tatggcaaga ctagaacctg
    45241 ctaaaattgc aaaaatagtt ctaggtattc ttttatatat aattgattta ataaaatcat
    45301 catttgtatt acccattcca aaagctgcta aaaaatctct gatattaatt tcttttgttc
    45361 caagctttaa tgacaaaaat attgtaataa gaagacctag gcagattaca aaaattatgc
    45421 ctaggtttat ttgtttttta tttcgcattt ttgcatgcat ttcctaaaag atttaggtat
    45481 tcttcaatag tatagtttat tgaaagtggt gttggagtgc atgaggctgc taacggtgta
    45541 ttatctggaa ttacagcaac ggcaccattt ttaattgcat ttattttacc taaaagagga
    45601 tctttttgta aagcttcaag agttttatca tcaccataag ttatgattac atcagcatca
    45661 tttatcttat ttgcttcttc tgcagaaatt tcctttgcaa aactatcttc actctcaaat
    45721 tcttttaatg attcagggaa aactagacct aaatctgtta aataatttgc tcttggatct
    45781 ttgctagtat aaatccagaa ttttgatgta tctgcagcat taatcatagt aaataatact
    45841 ttttttcctt tgatttttcc tttgatttct ggatgttttt ctaattcttt ggatatacga
    45901 gcttcagtat ttttgattaa ctcatcacct tctttttcca tacctaaggc ttttgaatca
    45961 attttaatca tatctctcca taaagtttgc caaggtttag atttgtatgc tgctacagga
    46021 gcaatttttg atagagtgtc ataatcttct ttagttatac cagaataacc tgctaagata
    46081 acatctggtt tagaatttga tattgcttca aagttaagtc catccaaatc gtcaaatagg
    46141 ttagctttac catttagttc tttgattttt tcttctgtcc atggtaaaac tcctttatca
    46201 gcacttacac cgtaatttgc ttttgaaaat ccaacaggaa ctattcctaa agctaatgct
    46261 acatcatgat ttccccaagc aatagttgca actctttcag gttttttatc taatatagtt
    46321 tgaccgaaag catgatctaa aactatttta tcaggcgcat gctctttaga agtattttct
    46381 tcatttttaa cagaattaga agaacaagcg ctaaaaacta tacacatagc taaaatagct
    46441 attagaaaaa atttgttttt cataaaaact ccttaaacat atttcaagtc tattgtatcg
    46501 ataataatta tcattgtcaa tagatacagt tacaaattta ataatataat tttttcaaaa
    46561 aaggatctag tttttatttt tagttaaatt atgtctatat ttttcattaa ctcatcaata
    46621 aaaatttgtt tgctaatatc tctttcttct gattttgttt caaggattat tgctacatca
    46681 ttcatatttt taggtaaata ccaaccatct ggcttatctg aatatccctt gaagcctaat
    46741 tgattaaaag tagttatttg accagtacct tgattgatgt tttcttcaac ttcattaaag
    46801 cctatacttc tcttgcataa tccctaactt gatcttctgt ccttaaagtc attttctatt
    46861 cctcgtcact aaaattatct tcaaaactaa taatatcatt aggagtgcaa gataaagcct
    46921 tacatattct ttctaaattc ttaaatgtaa ttggtttatc ctttcccatt tgtgccataa
    46981 catttgttgt aagtcctgcc atagctatta catctgtttt ctttagtcct ttttttgcta
    47041 actgtatcca taatggttta tagttaagtg ccataatatc cctctttttt tcttcattag
    47101 atttatttaa ttatagcata aatattgatt ttattcaatc ttacattgat ttagtgattt
    47161 gttttagaga atttgttgtg ttattccgtc cttattttgc cataaattgt tttaagtggg
    47221 acctcttatc cttaccattt ttacctccta attttgagta taaaaaagac gatagagttt
    47281 ttaatttcta tcgtctgaga tttacatatt aaattttcat cacaaaataa gactctttaa
    47341 agaatcttta aattcattgg tatgctgctt acaatatcca ttattaaaat ggatattgta
    47401 agcagcatac caactattta ttttttgagt tttaaacttt ggtagaagtc ttgaactcct
    47461 tatatttaca taactatttg cctctataac tgttatgaca cgaaaccaac aagcaagttc
    47521 aagcacctcc atatctttat tcaaatgagg actaagatat tcacaaactt tatcataaac
    47581 ctctttatct tttttcataa aaggggcata caacttagca aatttatcct aaaatttttt
    47641 gttgccttta tcaatcattt caacgctcct ttaaatataa aaatgatcct atcaatcttt
    47701 atattgagcc tattttctaa ttgatacctt gcaagaaagg aataatagct gttcctatac
    47761 caagtaggaa tgtaataaat gcagaaagtc atatactgtc attcttttct attaaatcat
    47821 tttgaagtgt ctcaaagcat ccatgatggc ttcttctttt tcaggtgtac actgtggaat
    47881 aatttgttta tcctttttag acttgttgta atgttctcgt aattctattc cacatttctt
    47941 ttttatctgt gcaatatata atgtcgaaac ttttaattca aatttattcc aaacatattc
    48001 tttgatttga gcatatgttg ctttgctctc cgcacttgtc aaatccatct catccagctc
    48061 aatttcaaca cttatgtgct tatcgacatc aagtttggac aaaagtgcta ccgtctcgac
    48121 gtgatgcgtt tgaggaaata gatccaccgg ctggactttc ttcaattcat atcccaattc
    48181 ttggtagagt ttgatatcac gcgccatggt tgcgacattg caggagatat aggcgatgcg
    48241 atcggctcct gtttgggcgc ttgctttgat aaagctttct gtcaagccct tgcgtggagg
    48301 atcaaccaag ataacggttg gttgaatccc atctttaagc caattcttca tagcattttc
    48361 agctgtgtca cagacatagt gggcgtttga aatattgttc agctgagcat tttttttact
    48421 attctcaacc gcttctggaa tcagttcaac accgtagact tctttgacat gcttggcgac
    48481 tgataaacca atggttccaa taccagaata agcatcaata accacgtcat ctttttttaa
    48541 ctctgcaaag tcaatggctg tttgatagag tttctccgcc atttcagtat tgacttggta
    48601 aaaggctggg ccagcgattt ggaagtcatt tcccaacatc tggtccgtaa tatagtcttg
    48661 accataaaga gtgcgccact ccttaccaaa aatcgcattg gtattctggt cgttgatatt
    48721 ttgcatgaca gacacaatct ctgggaactg cttgataact tgttcaatca attggtcaac
    48781 acgaaaaact tttggacgag ttgtcaccaa aacgaccatg atttgtcctg aatagtgacc
    48841 acgacgcacc acaagattcc gaatcaatcc agactgttcc ttttcgtcat aaggttttaa
    48901 atcaaaacga cggagcaggt ctcgaagagc tactacgact tggtcaatga caggatcctg
    48961 gataaagaaa tcttcaaggg gcatgaggtc gtgcgaattc ttacggaaaa atcctgtttc
    49021 caagacacca ttcactcgac gaacgggcac ctgcgcctta ttgcgatact tgactggatg
    49081 ttccatacca agcgtttcag caacttctac atctgcaatt ccagcaatct tgtagagact
    49141 gtccttgact tgcttggttt taaacttgag ctgttctgga taagaaaggt gtcctaaatc
    49201 cgcgattcct gaacgcaggt aagccaaatc tagatcttga ttacggtgtg gtgactggac
    49261 aaggtatttt tcaacttttc caaagccaat ctttttattg accttgagga cacgcatgag
    49321 aattttttca ctcggtaaag cattctctac aaaaaagacc aaaccatcta ccttggcaac
    49381 tcctgcccct tcatgggtca agtcaacaat ttcaacttct acaatatcat ttttctttaa
    49441 cattcttttc tctttctatt ggccgacaaa aaagacggta acgttacggt taccatccat
    49501 tataccatga aatttcttaa gaaccaaaac tcttgaagaa gttgacaagg gcttgccaga
    49561 gggagcttaa gaagttacca ccgtcctcta gcgccttatc cgcatcaaag ttaaggttga
    49621 tatttttaaa actgtcgcca gcttgtgata cgatgctttg tttaaggtca tttagggttt
    49681 tagtgaaatc tgcattgctg aggatatcac tctttgagag attcaaggca aaattgatga
    49741 tgatattgat ctggtttcct gttatgacct gatcaagttt gtaatttttt aaggtatctt
    49801 caacaatctt gcggatatct tcttctgtca gatttccctt gctttcttta gctttggcga
    49861 gtcctgactt gatatcagct agggcaacgt ttaatttatt agcatcatag cctgatttgt
    49921 ccttgttttc agcattgata tctgacaaag cttttagctc ttcttgagcc aaatctttat
    49981 tagcttgtgg caccttggct ccattagcct ctagcgaata gtaaatccct gctaaagcac
    50041 tttctcctgt aactggaata ggggctgcta cagtgatttt ggcatgttcc atacccagcg
    50101 ttactgctgc gtttcggtac atatcctgag tcaccttagt gatattttct ggtgtttcaa
    50161 tcttgacctc aagtggcgat ttgtcaccta gcttttgaat cttggctgat gaatacaact
    50221 gtaagctaga gtcattggcc acattcatga ttttagaata aacatcaggt gtcatggtct
    50281 tgagttcttt ggtatctgtt gaggcattgt agcccagttt tttaagagtt tgattttttt
    50341 ggtcttcaga tagggaggaa cctaggacat attcaggttg gacataggtt tcatcgataa
    50401 ctttttgaac atctgttgct gcatggacgc tattcatagc tgttactgcc cacaagaccg
    50461 cagcactagt cagaaagagt ttctttctca tagggaattt cctcctttac ttctttatag
    50521 taatatatct atcttaaaga aaacttataa caaaaacacc tggtctagcc agatgttgaa
    50581 aagagagtga aacatttgat gatgtaaagg ttaagtcgta cctgtctaga ataataataa
    50641 tttcctccat ttacatagag ttcagcaccg tgaaaaatgg aaatggggtg aatataacta
    50701 taagtctttc cagtcgtatt accaagcaag ggggcaacag tctcacgaga gtactgtttg
    50761 gctagagcca aggtattttc cttgccattt tgggcgataa aatcgatata ggcaggtcca
    50821 aaattatagg cttgaacagc tgtccagata tctacaccct tcttctgcgc cagatagaga
    50881 ttgcctgtca gagtttgaat gccttgccga atgctagagg cattatcatt gatggtgttg
    50941 gtggaaccac ttgcagactc actagactgc ataacatcgc cttcttttcc ttttgtttca
    51001 gtataaatca tagcaagcac aagctcttcg tttgctgggg tgtctttttc actcaagatt
    51061 tctcgcacca tgggttgata ggtcatgact tgtttgacat cttgatgaac gcggtaagct
    51121 ttatagccag caaaaaggaa gactgctagt acaagcactc ttcgaattcg tttaaacatt
    51181 atttactttg gatatcctcg atatttttga ttaagataga gtaggttcca ttttcgtttt
    51241 ggataaactc aacagactcg gcgtcttgat agacgttatt gggaacgatg agctcaattc
    51301 catttgataa ggagagtttt tggttttcaa atttctttaa ttggcgactt gcatcaattt
    51361 catcaaattg aacaggttct ggtacggctt ctttgacttg gtcaataaag ctcaaacgag
    51421 ccgtcagatt gttgtcaaaa aggtcattag ccaatttctc aggtgacaat tcattgcttt
    51481 cttctaggtt gttgaaaatc gctgatttga ccttggattg aaattgaaaa tcatctgtgt
    51541 taaaagattc agcaattctc tgggctgttt tttccagttc cttgatagat tttttaggag
    51601 aaatcttagg agcgacagca agaagattat ctgaaaaata gttcaaaaaa gtcccgttgt
    51661 acttgattcg tttttcaatc aggtgatact tgcgactctg aagattgacc accaaggcct
    51721 cgtcagcacc cgttccaaat ccaggcaggt tattctgagt cagcttgatt ggattatcaa
    51781 cttctcctcc gaggtgggtc aaggtctccc gcagggcaat tcgcaagaaa gcgaaatgtt
    51841 ctacaccttc tttagaaaat tgaacaaaaa tcaagtcatt ggtcttgaga ttttcagaaa
    51901 tgctaaactc ctctttccag agattagcca gcgttactga tgtctccaac aaatcgtctg
    51961 taatatgatt gaagaaggga ttttcttctt cgaaaatccc agtcttggct tcatctgaat
    52021 acacatgttc aattttttta cgtaggtatt cttcgatttt tggagtaata ttgagaaact
    52081 tatctgctaa gaacagctcg gtatcatccg gactgaactg gtgaataatg gctttcttaa
    52141 tataaatgtc cataaaagtt ttattcctcg tataatggga aggcatctgt caattctttg
    52201 actgcacttc tcacttcttc taatacagcc tcattttctg aattcttaag ggttttaatg
    52261 atgagttcag ccactttgcg actttcttct tcaccaaatc cacgtgcagt aatagctgct
    52321 gctccgatac gaatcccact tgtcttgaat ggtgacaagg tttcgtaagg gattgaattt
    52381 ttatttaggg taatattgac ttcatccagc aagttttgag caacttttcc gttttctaca
    52441 accttagtca catcaacaag gaagagatgg ttttcagttc cacctgaaat aatacggaaa
    52501 tcagggtctt gcaagaagac atctgccata gccttgctgt tcttaattac attggcagca
    52561 tattccttga aagctggatc caaaacttct ttgaaggaaa ctgccttagc agccacaaca
    52621 tgctccaaag gaccaccctg aataccaggg aaaatagctg aattgatttt cttagctaaa
    52681 tcctcatcat tggtcaaaat caaaccacca cgaggaccac gaagggtttt gtgggtcgtt
    52741 gttgtagtga tatgagcgta tggcactggg cttgggtgaa gaccagctgc aaccaagcca
    52801 gcgatgtggg ccatatcaac cataagctta gccccaacag catctgcaat ttcacggaat
    52861 tttgaaaagt caataatttg agaataggct gaagcaccag ctacaatcag ttttggtttt
    52921 acttcttggg cttgtttcaa gatagcatca aagtccaaaa gttccgtttc aggatccaca
    52981 ctataggaaa gaaagttgta ggtttgacca gagaagctaa ctggagctcc gtgggtcaag
    53041 tgtccacctg ctgccaaatc cattcccata accgtatcac ctggctcaat caaggacatg
    53101 taagccgcac agttagcttg gcttcctgaa tgtggttgga cattggcaaa tttagcaccg
    53161 aaaatttctt ttgcgcgttc aatagcaaga gtctctacaa cgtctactac atcagttcca
    53221 ccataataac ggcgtcctgg gtaaccctcg gcatatttat ttgtcaagat agacccttga
    53281 gctgccataa cagccttgga aactacgttt tccgaagcaa ttaactcgat attattttgt
    53341 tggcgttctt cttctttggc aatagcattc cagagatcag catcatatgc tttaaaatca
    53401 tctttgtcaa aaatcatagg tcttctcctt tattgtgtga ctagtccatt agtttgattt
    53461 tacaataaga aaatcaaact aaaagatgcg aataaaccgt ttctgcattt tatcacaagt
    53521 atagccaact ttttcataaa atgcatgagc acccagacga tgattggcag aatttaagcg
    53581 gataaaccca taaccacatc ttttggcttc ttgttccaac ccttgtagta aacttttacc
    53641 gataccttga ccttgcgctt gaggtgaaac tgctaaagct aagatattaa atcctgcttt
    53701 ggaatagagt gattcgtaaa cttcagcgtg gacatatcca agtaagacat gattagctgc
    53761 atcctcatag ccaagtagga aatgatggga atcctgagac agtctagcta gttggctagc
    53821 cgtttcctct ggactaaaag tataacccaa agcctcttgg ttgatgtcac atatcgcttt
    53881 cacatctgtt tcttgcaaat ctcttagcat ttcaagcctc ctcaaaagaa atctctggca
    53941 accgagcaag aatatcttct cgtttaattg cgccttggcg taagattttc accttgtctc
    54001 cagacaaatc cacaatagtt gaatcctgtc cagttagaaa agcatcgtct tccagaccca
    54061 gaacctcttg gtcaaaatcc ttcagaattt gttcaaaggt tacaccactt gcctgacctg
    54121 agatattggc agacggccca atcaagggac ctgtctctcg aattaaatcc agtgtgatag
    54181 ggtgactggg catccgaaat ccaatagttg caaggtcaga atttacccaa tagggaactc
    54241 ggtcattggc ttcgagaata atggtcaagg gacctggcaa aaaggtctct acaagttttt
    54301 gtagataagc tggctgattc ttagaaaagt gcaagatatc ctctaaagag gcaacattga
    54361 gattgagcgc cttgtctcta ggacgacgtt tgagctggta aacatggtca actgcttttt
    54421 cgtctaaggc cttggcaaaa agaccataaa ccgtctctgt aggcagaatg acagctccac
    54481 cattttccaa ctcttgtcta atcctgtcca tcatcaacca cgaccatcct atcttgacca
    54541 aattggtcct tgagtgttcg aactcttttt tcaggaagat gtttcctaaa aagttcggga
    54601 acactttgac cttgcttgta tccaatttca aggtaaatct taccaccatc cttgagatag
    54661 tcttttgcat cttccgcaat tctacagtaa atagctaggc catcctcgtc tgcaaagaga
    54721 gctagatgag gctccgaatg caaaacattc aagcctacct ctgactcatc ttcacgagag
    54781 atatagggtg gattggatac aattatatca tatttttcag aaatttctgc aaaacaatca
    54841 gattttttaa aaaatatatt aagattttga tcgctagcat tctctgatgc aagctctaag
    54901 gcctcttgag aaacatctgc tgctgtcact gaccaatctg gtctattttt tgctagagcg
    54961 agagcaatag ctccactacc tgttccaata tccagaactg agagattcgt ctcaagattt
    55021 tcagttagaa taagctccac taactcctct gtttctggac gaggaatcaa aacccgttca
    55081 tccaccttta aatgcattcc ataaaaatct gcctgtccaa tgatgtactg agctggcttg
    55141 tgagctgcta gttgctggta aatatcttct acaaattgtt tttcttcctc tgttgtcacc
    55201 tcctgctgga gggcaaaaat aaagtctgta aaagatagat ttttcagact acgatagaca
    55261 aaagagaggc tttccgcttc ctctccttgt cttatcaact cttcttcaaa atttgaaaat
    55321 aattgagcta atttcattat ttgtttaatt cttctagttt ttgtgtttgg tcataaagca
    55381 ccaaggcatc cacaacttcg tccaatttac cagacaaaat cgtatctagt ttttggaggg
    55441 tcaagccgat acggtggtct gtgacacggt tttgtgggaa gttataagtt cggatccgtt
    55501 ctgaacggtc accagtaccg attgtcgact tacgctcagc gtcttgttca tcctgagcaa
    55561 tctgagcaaa gtggtcagcg acgcgtgcac ggataatctt catagccttc tcgcggttct
    55621 tctgctgggt acgttcttcc tgcatctcaa ccttgatatt ggttggcaag tgaacgatac
    55681 gaacggcagt cgcaacctta ttgacgttct gtccaccagc accagaggcg tgatagatgt
    55741 cgacacgaag gtcttttgga tcaatgtcgt attcaacctc ttcaacttct ggcataacaa
    55801 gaactgtcgc tgtcgaagta tgaacacggc cttggctttc tgtcacagga acacgttgca
    55861 cacggtgggc acctgattca tacttaagct tagagtatac agactgacct gaaaccatag
    55921 caaccacttc tttaaaacca ccgacaccat tcatagaggc ttccatgact tcaaagcgcc
    55981 aaccttgggc ttccgcatac ttttggtaca tagttagcaa atctccagcg aaaagtgccg
    56041 cttcgtctcc accagctgct ccacggattt caaggatgat attcttgtca tcgtttggat
    56101 cctttggaag gagcaaaatt ttcagttttt cttcatattc ttctttttca gccttggcat
    56161 ctttgagttc ttgcttggcc atttcttcca agtccgcatc tccgcctgat tccttaatca
    56221 tctcttcggc atcgacgata ttttgaagga cttgtttata ctcacggtag gctattacgg
    56281 tgtcacgatt ggaagcttct tcttttgaaa gctccataaa acgcttggtg tctgaaacga
    56341 catcagggtc actcagcaat tctcctagtt cttcataacg gtcttctaca gcttgtagtt
    56401 gatcatagat gttcattttt tctccttatt tctcaattgt taaatcatag attgctacta
    56461 cttcattctc ggctatttcc ccagtttctt taaatccata actgaggtaa caaaatcttg
    56521 cctgttcatt ttctggttca taagacaacc aaagtttatt gcttaaacct gctggcgctg
    56581 ttcgaacata gtctagtact ttatccataa ttggtttaaa atatccttga ttttgaaaat
    56641 tcctatcaat cataaaacga aatagtaaat aatttccact actaattccg atctttttat
    56701 cataagctat catcacaaaa cctataattg catcattatc ataaactgcc aatgtagcta
    56761 caaaatctcc atttttagtg tagacgtatg cttcagctaa actaattgcg ttggttgcaa
    56821 tgaattgttt ttgatattcc ttgacatcca aatttaaaac atcaaaataa ttttccattg
    56881 taacatctct tagttcaatt gtcatagttt tgctccttgt tagaggttat cattggcgta
    56941 aaataatgtt tacggcaaac tgagatatag gtttcgttac caccaatctg gatctgttct
    57001 ccatcgtaaa cgggcagtcc atcctgtgtt cgcaacacca tggtcgcctt tttcttgcaa
    57061 tactgacaga tggtcttgat ttcgtcaatc ttgtctgcta aaagcaagag atatttggaa
    57121 ccttcgaaca attcattgcg aaagtcattt ttcaagccaa aagccatgac gggtatgtct
    57181 aactcgtcca caacacgagc taggtcgtaa acatggtggc gtttaagaaa ctgggcttca
    57241 tcgaccaaaa cacagtaagg tttttctggt aggtctcgga tatagccaaa gatatccgtt
    57301 gtttcctcaa tcgcaagggc agggcgtttc atgccaattc gactcgacac atagccaaca
    57361 ccgtcacgcg tatccagagc cgaggtcata atgacaacac cttttccttg ctcctcgtag
    57421 ttataggcca ctttgagaat ctcaatcgtt ttaccagagt tcatggtccc ataacgatag
    57481 tacaactgtg ccatgtttct tgcttcacgt ccatttctaa atttttgcta cattctagta
    57541 tatcataatt ttcttaagct ttaaacggca aaatgtggta aaatggaaga aatcaaaact
    57601 agtggaggaa gctattatgc catttgtacg catcgattta tttgaaggac gcacgctcga
    57661 gcaaaagaaa gctcttgcta aggaagtaac ggaagcagtt gtccgcaaca ctggagcccc
    57721 tcaatctgct gtccatgtca tcatcaacga catgccagaa ggaacttact tcccacaagg
    57781 ggaaatgcgt actaaataag ctagcttaag cagaattgct taggcttttt caatctccaa
    57841 gtagcattca ttgaagaaat atcctaaatt tgttacaatt tgaaaagaaa cttggagaat
    57901 ttccaagaaa agagctatta attaaaggaa acattatgat tacacgtgaa tttgatacca
    57961 tcgctgctat ctctactcca ctaggtgaag gggctattgg tattgtccgc ctgagcggaa
    58021 cagacagttt tgctattgcg caaaagattt ttaaaggaaa agacttgaac aaggttgcca
    58081 gccacactct caactacggt cacattattg atcctctgac tggtaaagtc atggacgagg
    58141 ttatggttgg ggctatgaag tctccaaaga ccttcactcg tgaggatatt atcgagatta
    58201 acacccacgg tgggattgcg gtgaccaatg aaattctcca gctagctatt cgtgaagggg
    58261 ctcggttggc agaacctggt gaatttacca aacgtgcttt tttaaacggt cgcgtagact
    58321 tgacacaggc agaggctgtg atggatatca tccgtgccaa gactgacaag gccatgaaca
    58381 ttgcggtcaa acaattagac ggctcccttt ctgacctcat taacaatacc cgtcaagaaa
    58441 tcctcaatac acttgcccaa gttgaggtca atatcgacta tcctgaatat gatgatgttg
    58501 aggaagctac tactgccgtt gtccgtgaga agactatgga gtttgagcaa ttgctaacca
    58561 atcttcttag aacagcccgt cgcggcaaaa tccttcgtga aggaatttca acagctatca
    58621 tcggacgtcc caacgttggg aaatccagcc ttctcaacaa cctcttgcgt gaggacaagg
    58681 ctatcgtaac agatatcgct gggacaacac gagatgtcat cgaagagtac gtcaacatca
    58741 atggtgtacc tctcaaattg attgatacag ccggtattcg tgaaacagat gacatagtgg
    58801 aacaaatcgg tgttgagcgt tcgaaaaaag ccctcaagga agccgacttg gttctactag
    58861 tgctaaatgc cagtgaacca ctgactgcgc aagacagaca acttcttgaa attagccaag
    58921 ataccaatcg cattattcta cttaataaaa ccgacctgcc agaaacgatt gaaacttcga
    58981 aactacctga agacgttatc cgtatttcag tccttaaaaa ccaaaacatc gacaagattg
    59041 aagagcgaat caacaacctc ttctttgaaa atgctggctt ggtcgagcaa gatgctactt
    59101 acttgtcaaa cgcccgtcac atttccctga ttgaaaaagc agttgaaagc ctacaagccg
    59161 ttaatcaagg tcttgagctg gggatgccag ttgatttgct tcaagttgac ttgactcgta
    59221 cttgggaaat cctcggagaa atcactgggg atgctgctcc agatgaactc atcacccaac
    59281 tctttagcca attctgttta ggaaaataag aaaaatccat gatccttcat tcggtcatgg
    59341 attttattgt ctttattagt aatctggtct taagacccct gttacagttg ccttagttgc
    59401 ttcgtagtcg ccatctacga caaccttgat aatgcgtttg gcatcttctt ctggtgctgg
    59461 aacaagaggt agacgagtgg gtccagcttc aaatcccata tagttaagaa ttgccttaac
    59521 tggagcagga cttggataag agaagagagc attaacctta ggaatgaatt tacgctgaat
    59581 tgctgcggct ttcttcatat cgctttctgc aatggcagta aacatctcgt gcatttcatc
    59641 cccatttgta tgagaggcaa cagaaataac cccatccgcc ccaaggttca tggcatggaa
    59701 agcatctcca tcctcacctg tataaatcaa gaactcttca ggcttgtgct caatcaagta
    59761 agccatatta gccaagctag tacattcttt gacaccgata atatttggat ggtcagccaa
    59821 gcgaagcatg gtttctggag tcaattcgac aactacacgc cctggaatgt tatagataat
    59881 aattggtagg tcagaagcat ctgcaatagt cttaaagtgc tgatacatcc cttcttgaga
    59941 aggtttgttg tagtaaggaa caatagcaag cccagctgcg aaaccaccaa attccgctac
    60001 ttctttgaca aactcaatag agtcacgcgt atcattagta cctacacccg caatcaaagg
    60061 aacgcgtcca ttgacaacct tttgtacagc cgcaaacaac tccaactcct catcgtgggt
    60121 caaagttgga ctctcagcag tcgttcctgc gagaagaatt ccatccgtat gatgggccaa
    60181 taaatgctca atcaaggctg gaatagcatc aaagttaatg gaaccatcct catggaaggg
    60241 ggtaataaag gctgtaatga ttttacattc ttttaaatct tgataagaca cgaaaacacc
    60301 tctctatttc aaagaaggag ttcatctgaa ctcctaaacg atatgactat tttaattcaa
    60361 atttcaattc ggctgttgga cgaaccaatc cacgttcatg aagagtttca gcaatctgaa
    60421 ctgagttcca agcagcacct ttgagaaggt tatctgaaac aacccacatg tgaattcctt
    60481 tttctgcatc caagtcttta cggatacgac caacaaaggt atcacgcgaa ccaactgcat
    60541 tgatagcttg aggatagatt tgatgagcta catcatcttc aagaacagca cctgggaagg
    60601 ctgcgatagc tgcttttact tcttcgattg gagccacttc ttttgtttcg atataaacag
    60661 actcagagtg agctgacaag actggaatac gcacacatgt tgcagatact gcaatgctat
    60721 catcttccat aattttctta gtttccttgg tcatcttcat ctcttcgtac gtgtaatcat
    60781 tatcagtgaa aacatcaatt tgtggaagag cgttaaaggc gataggataa tgtttcttgt
    60841 caccacctga aggcaagatt tccgcatgca aatcacgtgg tttcacacca tcattcaaaa
    60901 cctcacgaag ttcacgttgt gtctcaagaa ttgctcccat accagcacct gaaacggctt
    60961 gataagttga aacaatgata cggtccaagc cccatttttg gcgaaccggc tcaagagcca
    61021 ccatcatttg gattgttgaa caattagggc aggcaatgat tccgttgtga gcatcaagtg
    61081 catgagcatt gacctctgga acaaccaaag gaacatctgg attttgacgg aaataagatg
    61141 tattatctac tactaccacg ccagctttta ctgcgtatgg tgcatactta gctgatgtag
    61201 aactacctgc tgaaaagaga gcaatatcaa ctccttcaaa agctgtttca gtcgtttctt
    61261 caattgtaat atcttgatct ttaaatttca atgacttacc tgctgaacgt gcagaagcaa
    61321 ggtaacgaat tttatcgatt ggaagtgttg attcttccaa catttttatc atctgagcac
    61381 cgacagcacc tgtcgcgccg actacagcaa ctgtatatcc cataaataac ctctttcgga
    61441 attttctaaa aatttctata atagaagtat tatactactt tttccatgaa ttgcaaagct
    61501 ttttcatcat tttttggaaa ataaaaatcc cagtcgctag actagggact aagaggcatc
    61561 ttcatgtgat gagcaggttc acacaactca tcaaggtccg ctcctgcgtt atgacctcct
    61621 tatgctcaat agtagtccga agactaccta tcgactcatc gcatattcta tattactaga
    61681 gatacttgat tttgtcaata agaaattttt tcgatcgagt ataacaaaaa actctcgtat
    61741 tcaagagttt ccctatacat atcatgctaa ttgccgggat tgaaccggcg acctcatcct
    61801 taccatggat gcgctctgcc aactgagcta aatcagctta cttaagaagt atactatatt
    61861 cagaaaaact tgtcaagttt ccttagaaat tttccatagg aaaaagccag gtaagaacta
    61921 cctagcttat cttcttctat ttacttaaat caattcgacg accaatttta ccaataataa
    61981 tcgccccaat aatcaatgaa gcaaattcac cgattcctgt tgtaaaccaa gtaaggaaga
    62041 atggtaattc tgctacaata tttaactctg cagcgatagt aaacatggaa attgaaaaga
    62101 ggattgaaaa gaagaaatga tcttttcgaa tcaatccgtt aaagaggtaa tccttgctat
    62161 attttgcaaa aagccaaaca cctaaactga ggaatactaa ggttgaacca ccaccaacaa
    62221 agacatctag cagtccgaag ctaaagaaat tagcaatcat acaaccgatg gtaactccga
    62281 tgatgtactt aggattgtaa aaagccataa agttcatcat ttctgaaata cgaaactgat
    62341 aagcaccata gctgatggca tttaggggcg gtgtgacagt caaaacgacg tagatagcag
    62401 cgacgattgc aatatctgca acatcacgaa tagttaattt tttcatcttt tctcctttgg
    62461 cggttgcccg cgtgtaatat gcttggtgaa agaagctaag caccaagggt tgctttattc
    62521 aaccctacta gtatagcaca atagcctgct ttatgctata cttaacacat gaaaattcct
    62581 attcaaaaat tttttaacaa tgaaatctta gcctatctct tttttggcct tgcgacaact
    62641 ctagtttcga ttctctcacg actagtcatt tatcaactaa gtcatcagga actcctggct
    62701 actgccctag caaatattat cggtatccta tttgccttta tcacaaatga tacgattgta
    62761 tttaagcaaa aaaagaaaaa tagacttatc cgtttagtga aattttctct tgctcgcctt
    62821 tctacttttc tgttagactt gtttttaact tttctctttg taacccaatt tcctcacatt
    62881 atagggcaat tcgtaactga aaacattgat aagataaatg ctattgaaac aataattgca
    62941 caaatattca ttattatcat caattatatc ttgagcaagg cctatatttt tagaaaataa
    63001 tgctccattt atgagccttt taattgcctt ctataatgat ttaggataca atgaaacacg
    63061 aaacattgaa aggaaaacag tatgttaaaa aatctaaaat cgttcttgct tcgaggaaat
    63121 gttattgacc ttgctgtcgg tgttgtaatt gcctctgctt ttggtgctat cgttacttca
    63181 cttgtaaacg acattatcac tcctcttatt ttaaatcccg ctttgaaagc tgctaaagtt
    63241 gaacgtatcg ctcaactttc ttggcatgga gtcggctatg gtaacttctt aagtgctatt
    63301 atcaatttta tctttgtggg taccgccctc ttctttatta tcaagggcat tgaaaaagca
    63361 cagaaactga ctggcataaa gaaagaaaaa actgccgaaa aaaaaccaac cgaattggaa
    63421 gtccttcaag aaataaaagc tctccttgag aaaaaataat cgattaaaag gatctagcgc
    63481 aaagctaaat ccttttttct ttactctttg ggtaacttgc ctgctttttc aagcattttt
    63541 ttgatcagat aaggcatctt aacattctcc cgcccttttt tctcaatcag ttttttcaca
    63601 aattctggca tttgtaaatc ttcagattca gctaaaatgt ttttcgtctc gtctgaagga
    63661 actaactcat caaaggtctc ttcttctggg agaaattcct taaactcttg attttcattg
    63721 actctaactg tattgaccaa agcatcaatc aaacgagaat ctgtatttgg cattggtgaa
    63781 cgatggtagt ttaccccaaa ttcctggcat aagtcataac attctacatc attgtcaaac
    63841 aagacctcaa tatgctcact gatgaaacta ataggtacaa aaatataatg gtctggatgt
    63901 tctgtctgtt ctctgagata ctccaaaaca tctggtttaa tccatggaat accaatatca
    63961 ctctcactct gccaagtgtt ggtatattgt tctgaactca agcccagttt ttcagcgact
    64021 agcttgctat tttcgaaaat ttggtctata taaggatcac caaagtccaa ggcaaaaatg
    64081 ggcacactat gggctgaaaa gatgacttta aaactgtctt gctttacttc ttcttttaaa
    64141 atcttagcaa tttcatctgc ccaatagttt aagagcactt cttcctgata ccagtcctta
    64201 atgaccaaaa attgaatttg tttgctttct aaaaattttt catagcccat gacagagtag
    64261 aaagaataat ggggctccaa aatcaagcaa atacactgtt caattccgtc tgcttccatt
    64321 tgaccgatca catctggaat aaagggactg gaaaacttat tagcaaagta gacactgtat
    64381 tcattcccca gcctagcttc taccaaggta acctcttgac gggtaatttt ttgcagaggc
    64441 gtgcctccaa ttcgtacata attatcatag agagtctgaa tctcgtggtc ttggggtctc
    64501 accccacgac gaatatttgt gaaaaaatca gctacacctt caaaggtaat ctcttctggt
    64561 gaaccgaagg tcatcattaa aattgctttt ttcatgactg cttccttgtg atgtttttat
    64621 caagtttatt ttatcattaa ttcatcgata agtaaacgct aacataggga cattctcaat
    64681 cttctgtctt ttgtttttct cttctttcta tgatacaatg gaaaaaataa attcaaaagg
    64741 agttttttta tgacttatcc aaatctcttg gaccgcttct taacctacgt taaggtcaac
    64801 acgcgctctg atgaacactc tactactact ccaagtacac agagtcaggt tgacttcgca
    64861 acaaatgtcc taattcctga aatgaaacgt gttggactgc aaaatgttta ctatctaccg
    64921 aatggttttg ctattggaac cttgccagcc aacgatccgt ctttaacacg taagattggt
    64981 tttatatcgc acatggatac tgctgatttt aatgctgaag gagtcaatcc acaggtaatt
    65041 gaaaactacg atggtggtgt gattgaacta gggaattctg gtttcaaact cgatccagct
    65101 gacttcaaga gtcttgaaaa atatccagga caaacgctca tcacaacaga tggaacaacc
    65161 ttgctaggtg ctgatgacaa gtcaggaatt gctgaaatta tgacagccat tgaatatcta
    65221 actgctcatc ctgaaattaa gcactgtgag attcgtgttg gttttggtcc agatgaagaa
    65281 atcggtgttg gtgccaataa atttgatgca gaagattttg atgtggattt tgcctacact
    65341 gttgatggtg gtccactagg tgaacttcag tacgagactt tctcagccgc tggtgctgaa
    65401 ttgcatttcc aaggccgtaa tgtccaccct ggtactgcca aagggcagat ggtcaatgcc
    65461 cttcagctag caattgattt tcataatcaa cttccagaaa atgaccgacc tgagttaact
    65521 gaaggttacc aaggttttta ccatctaatg gatgtgacag gtagtgttga ggaggcgcgt
    65581 gcaagctaca tcattcgtga ttttgaaaaa gatgcctttg aagcgcgtaa agcatccatg
    65641 caatctatcg ctgataagat gaatgcagaa cttgggagct accgtgtcac tctcaacttg
    65701 acagaccagt actacaatat gaaagaagtc attgaaaaag atatgactcc aattaccatt
    65761 gctaaagccg ttatggaaga tctaggtatc acgcctatta tcgaaccaat ccggggtgga
    65821 acagacggct ctaagatttc ctttatggga atcccaactc cgaatatctt tgcaggtggc
    65881 gaaaatatgc acggacgttt tgaatacgtt agccttcaga ctatggaacg tgcagttgat
    65941 accatcattg gcatcgtagc ttataaaggc taaaaagacg aggtagctca gctacttcgc
    66001 ctttcttttt attctactgg tttttcttga tttccagtag ttgtagaaga ttctgttgtt
    66061 tcattttctg aagttgattc agcaggttta gaatctcttg tattgcttgg tttgttttcg
    66121 tcgctagcaa tttcaatgtt agattctgca gttgcgtttg gttggttctc agcactggtg
    66181 ttatcactat ttgcttcagc atttcttgct ggacttgttt cttcacttgc gctagctttt
    66241 gactggattt gatgattcaa aactagaata gcttttgtcg attcaagtaa agctgttttg
    66301 tctttactct tagcagaaag ttgatctaat aatgcatcca ccttatcaaa gtccgcatca
    66361 gatccattat tactttctaa ataagagtga agcgacatga gaatatcgta gagtttttga
    66421 tagagtacaa gtgtctgaga atcttgctca gcattttcct tttcttgttg aagggcgcta
    66481 gcgatacgag tcaagacatc ttttacctga ctgtttactt catccaagtc tgcatcagcc
    66541 ttgtttgtgg cagcttttag attttctact tcttctgcca aggattgtct gattccttct
    66601 tcatggattt gttccaagag ttgatttacc ttgctcaaaa gactttctac ttcttccttg
    66661 ctatctgtcg cagattattg gttgccatct accatgtact cctaaaacag gagagttata
    66721 atccaagatt acaaggcctt acagaaataa gaaatccaga taagacaatg ttcgtccaag
    66781 acgctattcg cttcgcacag cagcacggat tcaatatgct ttaattttaa agtttaggtg
    66841 tcaagacctc tttttagtgt gcccaaaatt tagagaagta atcaatcaac taacttttat
    66901 ttttttcaaa ctttcagtaa actgacctaa agctaactca atctgtcttt gtagatgctt
    66961 ctgctatcag ctagaagttg atctactttt gccaagactg ccttctcatc aaaagttcca
    67021 ggttgatagt tggattgcag ggatggaatc ttgtttttca aagccgcttc atatccctta
    67081 gtttgaacct tgatgtagtg attgtggtcg ccatgaggaa tcacaaaacc ttctgaatct
    67141 tcacttataa ttcgattggc atcaaaacca tgaccatctt cttcctcatg atggacatgt
    67201 agtgacggat tacttaatac agaactagaa gaacttccta cctcttccgt gttagagtgt
    67261 gatgggggat tgttaagaga tgacttagga atatagtgat agtgatcccc atgtcttact
    67321 atataagcat cacctgtatc tctgacaata tcattagggt taaagacata tgtggctgct
    67381 aattcacctg ccgacaagtc actctcagga atgaaatgat agtgaccacc atgtggtact
    67441 atagtagatt gaaatagaat atgagcaaat tgataagggg attttaaagt aatttctaac
    67501 aatgatttag aaactatgat gtgctattct aaattcaact cactatatat aaccatcatc
    67561 ggtagtataa cgtccctgta attttgctac agatacttct gcactagctc ctttatcgtc
    67621 tttaccatgt tcttgttttt ggcgattgat ttcatctttt gttcgtacat tttctgcatg
    67681 agcttgatct ttaaggtaaa cataatactt tccatctacc ttaataatat atcctccctt
    67741 aacctaactg acgatatctt gatctttcgg ctgatagttg ggggctttca ttaatagctc
    67801 ttcactaaag agcgcatcaa aaggaacttt accattatag tagtgataat gatcgccatg
    67861 agaagttaca taaccttgat ctgtaatctt aataacaatt tgttttgctt gaattccttc
    67921 tttttgacta acctagtctg gagtcaaatt ttcagtcttc ttagtgtctt tattactgtt
    67981 tacatatgaa acacgatttt tatctgtatt ggcctgttag ctatgttggt tcagagcata
    68041 aacacacaga cttaaggaaa ggataacaac agatccagct gctatatatt tctttttaaa
    68101 tttcataatt acctcatttc tataattatt tatatgatgt cttcattatt aaatgattaa
    68161 ataaattaat taaccaatta attaactagt aaatattcca cctcttttta agttgtatgt
    68221 caagaaattt tatatattaa taataaaatg aaattctccc aaagtcagag ttttatttct
    68281 aacttttgag agaacttcat ttttgattca gactttttct actgctattc cttacgctat
    68341 gagatcagat aaattctttt ttatcacttc tccacttggc aatcttaatt caatcgttcc
    68401 atccatattg aatataacac tatctaagcc taatccgtaa ctagctgtaa atttttctaa
    68461 tttttcttgt acaggatcta ctgctggagc ttcctctaat gctggatcta acatagggtc
    68521 actccccaca ttcccttctg gattcaacat tccattatcc gttgagtttt ctggttttac
    68581 aggtttttcg tttggtgcct ctggtaaaga atctgctggt ttattttctg ttggttggtt
    68641 ctcaactgtt ccagtagata cttttccagt ttcagatggt ttattttcac catttccttg
    68701 aggtgcttct cctgtaaaat ctgccatatt ctttttaatg acttctcccg atggtaaata
    68761 taattcaatt gttccgtcca tattaaacaa gacattttct agcttcatcc cataactttc
    68821 agcaaatttt gctacttttt cttgtacagg atccactgta ggaacttctt ctaacgttga
    68881 atcactagta ctgttcccag tttcaggaag cttttctttt tctaccttct cactagtctt
    68941 tggttcttct accttttcat caagttttga gttttcttgt gctttattcc ttttaaattg
    69001 tggtagaata cttggtttat cagtttgatt ttctttttcc aagataggta cttccacaat
    69061 ataagtcgat tgattgtcca aataagcatt tgccatgaag gttacaggaa ttttatttcc
    69121 ggccgttctg gttgttcctt ggtttaattt cggaatcggt aatttaattt caccaacttt
    69181 atagttattt tctaaataag catttccatg aaattcatca aacactctga ctaaagcatc
    69241 agttccttta ggcactgcaa attgagggtt cactcttaaa taagtatccc ctgcatggaa
    69301 aggatagaaa atcgtttgac tggccatttt gtaagctaaa gaggttggaa ctgtaaatgt
    69361 accatcataa cttacttctg gataatcttt tgaagcgata gtatacttaa atgtttgtcc
    69421 tggtaaataa ggttgatcta attcaaagtt tgcaatattc cctactcctt ctccaaatac
    69481 tttaccagat actttctcca atacttttcc atctggtgtt attaatttta ctagcatatt
    69541 gatacctaat tttttttcca attcaggcgg aaaactaaaa gaaacgcgtt tttgaccatt
    69601 ggctagagta aagttttgat tattaaacgt actatttttt aacaaattaa caacattcgt
    69661 taattcttct ccagtataaa ctttattccc ttctttttta gcaactcctt cttcgggttt
    69721 aaacagttca tagttactgt gagaatgacc aattccaacc ggtttatgtt catcaatcgg
    69781 atctgcatga tggtgatctc catgcggata aataatcgca tttttttctt tattcacgac
    69841 aatactttca cgtttgacac catattgttt cataatgcca gcaatttttt cttcgatttt
    69901 tttatctaaa tctttcattt ctttggcatt acctggataa tcctgttcat gagatgacaa
    69961 agaatctaat ccattatgac tagttttaac ttcctctaaa tgtttttgcg cagccttaat
    70021 ttgctcttct gtcaagtcct tcttgaagaa ataatgattg tggtctccgt gactcatgac
    70081 aaaacctgat tcatcttcag cgataatacg attagcatca aatccgtatc catcttcttc
    70141 atgtttctca tgtgaagttc ctggattgat tggaagagat ggagaaggtg ttgctagact
    70201 attgtttgga agagtcggtt gcccaatttg atttgatttt ggaatgtaat ggaaatgatc
    70261 accatgtctt acaatataag ctgtagccgt ttcttcaacg atatcttttg gattaaaaat
    70321 ataaccatca gatgctgaag agagtttctt acttgtcgtt aaagaagaag gattgcttga
    70381 aagactgcct agactagaca ctacttcatt aggttttgca tttgtagaaa ctgtagaacc
    70441 agttccactg ataggcacca ttctggcaat cttttcttct aaggcagaaa gcttgctgta
    70501 aggaataaag tggtaatggt cgccatgcgg aatcgcaact ccatttggtg tacgactgat
    70561 aatcttagca gggtcaaaga ccaggccatc tgattcactg taacgttggg cgctaggtga
    70621 atcatagagt tccttcaaaa gactctggag attttcagat ttatttgctg gcttgctagt
    70681 tgatcctttt gctacagatt gcgtgttatt gtcactagct gttgaagaat agcttaactg
    70741 actcggttgc atattttttc cagccagatg tgctttagct gctgctaatt cactagcaga
    70801 taaatcgctt ttgggaatgt agtgatagtg acctccatga ggaacgatat aagcattacc
    70861 cgtatcttcg ataatatcag ctggattaaa gacataacca tcatttgtcg tatatcgtcc
    70921 ctgagacctt gctacagcaa cattagagtt aaccttctca ttatctttga catgttcttg
    70981 tttttgacga ttgatttcat ctttagttcg aacattatca gcatgagctg catctttcag
    71041 gtagacataa tattttccat cgaccttgat gatataacca cccttgactt cattgacaat
    71101 atcagcgtct ttaagttgat agtttggatc cttcatcaag agttcttcac taaagagggc
    71161 atcataagga actttcccat tatagtaatg atagtggtca ccgtgtgacg ttacatagcc
    71221 ctgatctgta attttgatta caatttgctc agcctgaatt ccttctttct ggctaacctg
    71281 gtctggtgtc aagttttcac ttttctgact tgactggctg ccatccacat aagagacacg
    71341 attattgtcc ttattttcct gcgaacgatg ctggtttagt gcataggcac atagactcaa
    71401 ggatacgata acagctgatc cagctgctat atatttttta ctaaatttca taaatccctc
    71461 atttcaataa atgatgaagt ttttttctca acttctttta ctttattaaa tagttttcta
    71521 aaccgattat ttaaccattt aattaaccag taaatagtct actatttaaa attacttttg
    71581 tcaagtttct gttacgtttt tctaaaaaag agaagtaaca cgaaaggtta cttctccata
    71641 aaatatgctc cattcatttt actactgtat aggagccggt tgactttctt ttaacaaagc
    71701 caagagacta tctacttctg ctgaaatagt attattatct ttcgttccga gaagaagact
    71761 actttttaga ccagtcaatg tctccatagc attttgtcta atactagaat ctgttacttt
    71821 ttctagcaag gcctccgcct ctgctatctt agcgttaata acagaatgct ctacttgagg
    71881 aatttcagcc tcatctgtag tatcttcagc ttcttcctct gtctcttccg tatcagtgct
    71941 tggtttataa agattatctg ctgaaggatt taaaccagcg tgattctctt tttcatcaga
    72001 ttcagggtga gttggctcac ttacttcatc atgttcctta tcttcctcag gtttttctgt
    72061 ctgaggtttc tccgtttgat tggtatcagc ttgaccattt ttgtttcttt gaacatggtc
    72121 gctagcgtta ccaaaaccat tatctgaatg cggacgttcg tttggatgtt cgacatagta
    72181 cttgacagtc gccaaaagat cctcaagagt atacccctta ggtgcctcat aaaggccttc
    72241 gtcaaaccac tcaaatttga tgttatggta atggtcataa tgaggtatga ttaaactacc
    72301 gtttttgact tctacagtat attgaagatt gtaaggcaca cgatcaagtg gcaccttctt
    72361 agctgctttc acgcggttgt agatagcttc tgctcctttt gcctcagtat ttcctgaatc
    72421 ctgatg
//
LOCUS       DAVBNO010000003        70094 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVBNO010000003 DAVBNO010000000
VERSION     DAVBNO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 70094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70094
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.003"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            624..1265
                     /locus_tag="VQ846_000153"
     CDS             624..1265
                     /locus_tag="VQ846_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET3074813.1"
                     /translation="MTIESGQRIALVGQPGSGKSTLSKIPSGLYKIDTGKVLFDGVNI
                     NQIDKKILSQNLGVVPQDSFLLNRSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMP
                     MKFNTIISEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEERITKYIKS
                     QGCTQIIVAHRLSTIKDADIIVVMKGGKIVESGNHKYLMDLGGEYYSLYTKRK"
     gene            1278..1448
                     /locus_tag="VQ846_000154"
     CDS             1278..1448
                     /locus_tag="VQ846_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074814.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(1944..3290)
                     /gene="gdhA"
                     /locus_tag="VQ846_000155"
     CDS             complement(1944..3290)
                     /gene="gdhA"
                     /locus_tag="VQ846_000155"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET3074815.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGIICVSEGANMPSDLDAIKVYKENGILYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDDRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            3822..4278
                     /locus_tag="VQ846_000156"
                     /pseudo
     CDS             3822..4278
                     /locus_tag="VQ846_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            4281..5293
                     /locus_tag="VQ846_000157"
                     /pseudo
     CDS             4281..5293
                     /locus_tag="VQ846_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182321.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            5268..5846
                     /locus_tag="VQ846_000158"
     CDS             5268..5846
                     /locus_tag="VQ846_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HET3074816.1"
                     /translation="MVRNYWKARSWLLVLALSFLILSPVGAQESLSSYFVKITDTSKA
                     VKNGNQSEAQKLVQEMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVV
                     RDNSIAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(5903..6187)
                     /locus_tag="VQ846_000159"
     CDS             complement(5903..6187)
                     /locus_tag="VQ846_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET3074817.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(6241..7176)
                     /locus_tag="VQ846_000160"
     CDS             complement(6241..7176)
                     /locus_tag="VQ846_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET3074818.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVSAPGRIDSVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            7280..7723
                     /locus_tag="VQ846_000161"
     CDS             7280..7723
                     /locus_tag="VQ846_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET3074819.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVG"
     gene            7839..8105
                     /locus_tag="VQ846_000162"
     CDS             7839..8105
                     /locus_tag="VQ846_000162"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET3074820.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVGSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            8102..8476
                     /gene="crcB"
                     /locus_tag="VQ846_000163"
     CDS             8102..8476
                     /gene="crcB"
                     /locus_tag="VQ846_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET3074821.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            8470..8799
                     /gene="crcB"
                     /locus_tag="VQ846_000164"
     CDS             8470..8799
                     /gene="crcB"
                     /locus_tag="VQ846_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET3074822.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQVSKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            8918..9265
                     /gene="rplS"
                     /locus_tag="VQ846_000165"
     CDS             8918..9265
                     /gene="rplS"
                     /locus_tag="VQ846_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET3074823.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            9307..9378
                     /locus_tag="VQ846_000166"
     tRNA            9307..9378
                     /locus_tag="VQ846_000166"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9340..9342,aa:Arg,seq:cct)
     gene            complement(9544..10542)
                     /locus_tag="VQ846_000167"
     CDS             complement(9544..10542)
                     /locus_tag="VQ846_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET3074824.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNKLECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLKVVELKTILSKHN
                     LATSGKKAELITRIINNINVDKLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDISMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(10585..11391)
                     /gene="yidA"
                     /locus_tag="VQ846_000168"
     CDS             complement(10585..11391)
                     /gene="yidA"
                     /locus_tag="VQ846_000168"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET3074825.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGTYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKSNDESGVAHAIRTWVL"
     gene            complement(11404..12708)
                     /locus_tag="VQ846_000169"
     CDS             complement(11404..12708)
                     /locus_tag="VQ846_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET3074826.1"
                     /translation="MNEKVFRDPVHNYIHVINQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMIAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQSPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPKDKDFFARTSPHLLPFFEKNVTLSD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLYLIENDHFTPNKN"
     gene            12780..13157
                     /locus_tag="VQ846_000170"
     CDS             12780..13157
                     /locus_tag="VQ846_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET3074827.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKNSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            13248..13580
                     /locus_tag="VQ846_000171"
     CDS             13248..13580
                     /locus_tag="VQ846_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET3074828.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            13592..15163
                     /gene="ffh"
                     /locus_tag="VQ846_000172"
     CDS             13592..15163
                     /gene="ffh"
                     /locus_tag="VQ846_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET3074829.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPN
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15358..16641)
                     /locus_tag="VQ846_000173"
     CDS             complement(15358..16641)
                     /locus_tag="VQ846_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET3074830.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(16895..17608)
                     /gene="rsmG"
                     /locus_tag="VQ846_000174"
     CDS             complement(16895..17608)
                     /gene="rsmG"
                     /locus_tag="VQ846_000174"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET3074831.1"
                     /translation="MKPETFYNLLAEQNLPLSNQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            17702..18262
                     /locus_tag="VQ846_000175"
     CDS             17702..18262
                     /locus_tag="VQ846_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET3074832.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            18264..19163
                     /gene="htpX"
                     /locus_tag="VQ846_000176"
     CDS             18264..19163
                     /gene="htpX"
                     /locus_tag="VQ846_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET3074833.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(19207..20766)
                     /locus_tag="VQ846_000177"
     CDS             complement(19207..20766)
                     /locus_tag="VQ846_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET3074834.1"
                     /translation="MHYEHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIHSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLELVNLEDL"
     gene            21491..22033
                     /locus_tag="VQ846_000178"
     CDS             21491..22033
                     /locus_tag="VQ846_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET3074835.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            22033..22662
                     /gene="nth"
                     /locus_tag="VQ846_000179"
     CDS             22033..22662
                     /gene="nth"
                     /locus_tag="VQ846_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET3074836.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            22873..23394
                     /gene="pyrR"
                     /locus_tag="VQ846_000180"
     CDS             22873..23394
                     /gene="pyrR"
                     /locus_tag="VQ846_000180"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET3074837.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            23413..24336
                     /locus_tag="VQ846_000181"
     CDS             23413..24336
                     /locus_tag="VQ846_000181"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET3074838.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            24386..25465
                     /gene="carA"
                     /locus_tag="VQ846_000182"
     CDS             24386..25465
                     /gene="carA"
                     /locus_tag="VQ846_000182"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET3074839.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            25778..28954
                     /gene="carB"
                     /locus_tag="VQ846_000183"
     CDS             25778..28954
                     /gene="carB"
                     /locus_tag="VQ846_000183"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET3074840.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELSVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKDVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(29229..30038)
                     /locus_tag="VQ846_000184"
     CDS             complement(29229..30038)
                     /locus_tag="VQ846_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET3074841.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(30040..30843)
                     /locus_tag="VQ846_000185"
     CDS             complement(30040..30843)
                     /locus_tag="VQ846_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET3074842.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(30853..32340)
                     /gene="tacF"
                     /locus_tag="VQ846_000186"
     CDS             complement(30853..32340)
                     /gene="tacF"
                     /locus_tag="VQ846_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET3074843.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFIDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVVPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            32676..33383
                     /locus_tag="VQ846_000187"
     CDS             32676..33383
                     /locus_tag="VQ846_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET3074844.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            33388..34410
                     /locus_tag="VQ846_000188"
     CDS             33388..34410
                     /locus_tag="VQ846_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET3074845.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            34427..35296
                     /gene="licA"
                     /locus_tag="VQ846_000189"
     CDS             34427..35296
                     /gene="licA"
                     /locus_tag="VQ846_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET3074846.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            35286..36164
                     /locus_tag="VQ846_000190"
     CDS             35286..36164
                     /locus_tag="VQ846_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET3074847.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            36176..36865
                     /locus_tag="VQ846_000191"
     CDS             36176..36865
                     /locus_tag="VQ846_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET3074848.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            36961..37809
                     /gene="dprA"
                     /locus_tag="VQ846_000192"
     CDS             36961..37809
                     /gene="dprA"
                     /locus_tag="VQ846_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET3074849.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDV
                     LAEFEF"
     gene            38693..39736
                     /locus_tag="VQ846_000193"
     CDS             38693..39736
                     /locus_tag="VQ846_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET3074850.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEHFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            39903..41990
                     /gene="topA"
                     /locus_tag="VQ846_000194"
     CDS             39903..41990
                     /gene="topA"
                     /locus_tag="VQ846_000194"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET3074851.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            42211..42570
                     /locus_tag="VQ846_000195"
     CDS             42211..42570
                     /locus_tag="VQ846_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET3074852.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            42663..43295
                     /locus_tag="VQ846_000196"
     CDS             42663..43295
                     /locus_tag="VQ846_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET3074853.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            43339..43995
                     /locus_tag="VQ846_000197"
     CDS             43339..43995
                     /locus_tag="VQ846_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET3074854.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            44014..44646
                     /locus_tag="VQ846_000198"
     CDS             44014..44646
                     /locus_tag="VQ846_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET3074855.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            44674..45108
                     /locus_tag="VQ846_000199"
                     /pseudo
     CDS             44674..45108
                     /locus_tag="VQ846_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
     gene            45120..46157
                     /gene="leuB"
                     /locus_tag="VQ846_000200"
     CDS             45120..46157
                     /gene="leuB"
                     /locus_tag="VQ846_000200"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET3074856.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VEWIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFSDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIYGSA
                     PDIAGQGIANPISMILSVAMMLRDSFGGYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            46154..46423
                     /locus_tag="VQ846_000201"
     CDS             46154..46423
                     /locus_tag="VQ846_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET3074857.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            46427..46786
                     /gene="leuD"
                     /locus_tag="VQ846_000202"
     CDS             46427..46786
                     /gene="leuD"
                     /locus_tag="VQ846_000202"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET3074858.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            47005..47148
                     /locus_tag="VQ846_000203"
     CDS             47005..47148
                     /locus_tag="VQ846_000203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074859.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            47248..47421
                     /locus_tag="VQ846_000204"
     CDS             47248..47421
                     /locus_tag="VQ846_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074860.1"
                     /translation="MTDVRGTSCFVIKFGKADEQLAAKLWEEGKMVYASSTSMIKRLK
                     LAMRARCNGVYGR"
     gene            47411..47572
                     /locus_tag="VQ846_000205"
     CDS             47411..47572
                     /locus_tag="VQ846_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074861.1"
                     /translation="MVDKNGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQVEYY"
     gene            47735..49666
                     /locus_tag="VQ846_000206"
     CDS             47735..49666
                     /locus_tag="VQ846_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET3074862.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEKLLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYSGGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            49653..50969
                     /locus_tag="VQ846_000207"
     CDS             49653..50969
                     /locus_tag="VQ846_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET3074863.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSNVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            51144..52130
                     /locus_tag="VQ846_000208"
     CDS             51144..52130
                     /locus_tag="VQ846_000208"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET3074864.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            52194..52265
                     /locus_tag="VQ846_000209"
     tRNA            52194..52265
                     /locus_tag="VQ846_000209"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52227..52229,aa:Arg,seq:ccg)
     gene            52448..53146
                     /locus_tag="VQ846_000210"
     CDS             52448..53146
                     /locus_tag="VQ846_000210"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET3074865.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFNQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVIIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            53137..56676
                     /gene="smc"
                     /locus_tag="VQ846_000211"
     CDS             53137..56676
                     /gene="smc"
                     /locus_tag="VQ846_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET3074866.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELTQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDVIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDQMATLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEKDRLSDGDWQADKENCQERLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNFQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVRNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            56673..57467
                     /locus_tag="VQ846_000212"
     CDS             56673..57467
                     /locus_tag="VQ846_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET3074867.1"
                     /translation="MTIKLVATDMDGTFLDENGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            57467..58285
                     /locus_tag="VQ846_000213"
     CDS             57467..58285
                     /locus_tag="VQ846_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET3074868.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKSTLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGHEDEYTITFNGGLVQKNTGEILDKTVFSYADVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            58289..59578
                     /gene="ftsY"
                     /locus_tag="VQ846_000214"
     CDS             58289..59578
                     /gene="ftsY"
                     /locus_tag="VQ846_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET3074869.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEMEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPNSQEVTEEEKLEHE
                     GTVEENNSEVLEPEAPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDVLRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(59624..61111)
                     /locus_tag="VQ846_000215"
     CDS             complement(59624..61111)
                     /locus_tag="VQ846_000215"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET3074870.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGIKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(61260..62000)
                     /locus_tag="VQ846_000216"
     CDS             complement(61260..62000)
                     /locus_tag="VQ846_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET3074871.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(62000..64165)
                     /locus_tag="VQ846_000217"
     CDS             complement(62000..64165)
                     /locus_tag="VQ846_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET3074872.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            64338..65291
                     /locus_tag="VQ846_000218"
     CDS             64338..65291
                     /locus_tag="VQ846_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET3074873.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            65368..67356
                     /gene="uvrB"
                     /locus_tag="VQ846_000219"
     CDS             65368..67356
                     /gene="uvrB"
                     /locus_tag="VQ846_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET3074874.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            67366..67929
                     /locus_tag="VQ846_000220"
     CDS             67366..67929
                     /locus_tag="VQ846_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3074875.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            67895..68383
                     /locus_tag="VQ846_000221"
     CDS             67895..68383
                     /locus_tag="VQ846_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3074876.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDDTIQAGGIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            68457..68924
                     /locus_tag="VQ846_000222"
     CDS             68457..68924
                     /locus_tag="VQ846_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET3074877.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(68921..69436)
                     /locus_tag="VQ846_000223"
     CDS             complement(68921..69436)
                     /locus_tag="VQ846_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET3074878.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(69448..69975)
                     /locus_tag="VQ846_000224"
     CDS             complement(69448..69975)
                     /locus_tag="VQ846_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET3074879.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
BASE COUNT        21430 a        12515 c        15247 g        20902 t
ORIGIN      
        1 aaaagcgtcg ttaatgatgc attaaagaac agtcgagatg aaaaaatcat gtgacgcacc
       61 aagggaggag tctgcccttt tgaggaaatc tagcgaggaa aaacgatact ggaacagcag
      121 aaagtaaaac tgacctcatg aggaggaaga aagtggctca tgaggtcagg ggttttgtaa
      181 gttacatcta gttgagagag gtatgaatga tttgggttta atcagatggt ttacctcctt
      241 ttcatcggta ttcaccactt tttcaatagt aaagacgaga tggtcttgcg gcaagaaaga
      301 actgatttct agtggtaaag ttgtttgatt tgtgttatag tgaatatgca tgggatagcc
      361 cttctaaatg gtttattggg caattctatt ttaccaagac tatcgcaacc atgcaaaaaa
      421 gcaacggcaa atccgcttct tttttggaga taagagacta ttgtcccaga ctcttagtac
      481 tggttggagg atacgatgaa agatagttat tggttaaatt attttccgga gtatagttta
      541 gaaacgtttg aagtagaata gatattaagg acctatcttt ctcttattcc aaagactcag
      601 ctcctgtaat agaaaattta aatttgacta ttgagtcagg acagagaatt gcactagtcg
      661 gtcaaccggg gtcaggaaaa agtacattat caaaaatacc atcaggatta tataagattg
      721 atacaggaaa agttttattt gatggtgtaa atattaatca aatagataaa aaaatattaa
      781 gtcaaaattt aggagtagtt ccacaggatt cgtttttatt gaacagaagt attcttgata
      841 acataacttt aaagcacgaa gttacttcac aaaagataga ggaagtttgt aaagcagttc
      901 aaatctatga tgaaatcatg gctatgccga tgaaatttaa tactatcatc tcagagatgg
      961 gttcaaatat ttcaggtggt caaagacaac ggatagcact ggcacgtgca ttaataaata
     1021 atcctagtat tgtaatttta gatgaagcaa ctagtgcttt agacactatt aatgaggaaa
     1081 gaataacaaa gtatataaaa agtcagggct gtactcaaat aattgtagct catagattgt
     1141 caacgattaa ggatgcggat attattgttg taatgaaagg tggtaagatt gttgagtcag
     1201 gaaatcataa gtacttaatg gatcttggtg gagagtacta cagcttatat acaaaaagga
     1261 aatgaggtgt aaagaaaatg aagaaagaaa atgaatatgt aattttaaca acagcctcac
     1321 taggggtgat gattggaata gtgtttgcaa tttttttaga ttttccagtt gaatatggta
     1381 tttctttagg attgttgaat ggaatagtat tgggttcgct gattgtttac aaaaacaata
     1441 agaattaagc ataatttttt gctgtaaact aaggagtaga gatggctata gttgaaatta
     1501 taaatctaac aaaaagcttt aaagatattg aagttattca taacacttaa ataatagagc
     1561 aactacagta gtagcttaaa aacatgatta aatcgctatt cttaggagta gcgttttttc
     1621 tttttgttta atactctttg aaaatctctt caaaccacgt cagctttgcc ttgccgtact
     1681 caagtacagc ctgcgactcg cttcctagtt tgctctttga ttttcattga gtataaaaag
     1741 ggttaagtaa gtatagtaaa ttgaaataag atatgaacaa atcgattaga aaagtcaaat
     1801 taatttctag aaatatgtta gaaattggtt tgaattccgc aatcaatttg ttcagttttt
     1861 atttcattta attagaattt cccaactgtc taactcaact aaaaatcccc aatcgtagtg
     1921 attgaggatt aagcaaataa atcttaaaca ataccttggg caatcatagc gtttgctaca
     1981 ttttcaaagg cagcaatgtt agctcctgca aggtagtctt tatcaagacc gtatgtttct
     2041 gaagtcgttt tagctgtgtt gaagatgttt gtcatgatgt ctttgagacg gtcatcaact
     2101 tcttcacgag tccatgagag gcgaagactg ttttggctca tttcaagagc tgaaacggct
     2161 acaccaccag cgttggcagc ttttgcaggt ccgtataaga taccattttc tttgtaaact
     2221 ttgatggcat caaggtcgct cggcatgttg gcaccttcag atacacagat aatgccttga
     2281 gcaaccaaac gtttagctgc ttcaccgttg atttcgtttt gagtggcaca tggaagagca
     2341 atgtcatagt ttccagcgta agtccataca gtaccttcgt ggtaggttgc agttgctttt
     2401 tcagctgcat actcagtcaa acgagcacga cgtttttctt taacatcaac caaaagatcg
     2461 aagtcgatac cattttcatc gatgacataa ccatttgagt cagaaacaga aataacagtt
     2521 gcaccgagtt cagttgcttt ttgaagagca tattgagcaa cgttaccaga acctgaaata
     2581 acgactttct taccagcaaa gctgttaccg ttagctttga gcatttcttc agtatagtaa
     2641 accaaaccgt aaccagttgc ttctggacga atcaagctac caccaaatcc aagaggttta
     2701 ccagtcaaga caccagcatc aaattggtta agacgtttgt attgaccgta aaggtaacca
     2761 atttcacgtc caccaacacc gatatcacca gcaggtacgt caagtgatgg tccgatgtgt
     2821 ttttgcaatt cagtcatgaa gctttggcag aagcgcatca cttcagcatc tgttttacct
     2881 ttaggatcga agtctgatcc acctttacct ccaccgatag gaagtccagt caagacattt
     2941 ttaaagattt gttcaaatcc gaggaatttc aagatccctt ggtttacagt tgggtggaaa
     3001 cgaagtccac ctttgtatgg tccaacagct gagttgaatt gaacacggta accacggttt
     3061 acttgaattt ttccatcacg gtcaacccaa ggaacacgga aagaaaccac gcgctcaggc
     3121 tcagtaatac gtgccaagat attttcttcg atatactcag ggtgtttttc aaatacaggt
     3181 tctaaagtgt tgaaaaattc ttcaacagct tggaggaatt cagcctcgtg cccgttacga
     3241 gctttcacag tttcaaacac gctttggata tattctttag cagatgtcat atcgttctcc
     3301 ttttattgtc atattttatt acataagaca ttatagcaga attttttttc actgtaaaga
     3361 aaaaacagaa attttctaaa aattctttca attcagtttt ttggtttttt tgaaaataga
     3421 taaaaaaacg aacgtttttc ttggaaatat ctttctcaaa gacaaaatct taaagtatgc
     3481 tataagaaaa gaaaatgttt agcaattcag aaatcacttg ttaatttatc cgtgatatgt
     3541 tagatttgcc agtaaaacta tgaccatttt ggagaaaagc aatatttaga ctagactaaa
     3601 ataaaaaagt attatataat agtgatatga aatcaactaa agaagaaatc caaaccatca
     3661 aaacactttt aaaagactct cgtacagcta aatatcataa acgccttcaa atcgttctat
     3721 agtaaaacga aacaagaaca gggcaaatcg atcaggacag tcaaatcgat ttctaacaat
     3781 gttttagaag tagaggtgta ctattctagt ttcaatctac tatatttcgt ctgatgggca
     3841 aatcttataa agagattata gagcttttag actgtaatca aacaacgatt tggcgaaatg
     3901 taaaaaaata tgaggagttc ggactcgact ctctctttca agaaacacgt ggtggtcgta
     3961 accatgcata tatgacggtt gagcaagaga aagcctttct tgctcgccat ttgaaggctg
     4021 cagaggcagg agaatttgtt acaattgatg ccttatatca ggcttataaa aaggagttag
     4081 gtcgttccta cacacgtgat gccttctatc aactgttgaa gcaccatggt tggcgaaata
     4141 ttatgccacg tccagaacat cctaggaaag cagacgctca aaccattgtc gcgtctaaaa
     4201 ataaaatctt aattcaagaa gacaagaaag cgctttaaat atagtagacg ttttcgtaag
     4261 gttcgcttga tgtaccaaat agtacgccca tgcaaaagaa tatctatttt gttgtcttgg
     4321 acctgcatac gacggataga gataaaatta tccagctatt caaggattgg acggattata
     4381 gtgccaagtt ggtcgagggg gaattggtta aaaaagatgg acagaatgct ctctttcctc
     4441 cgagcgatac tggtgagaca gttgggctca atcctcatcg tttgacgctg acttttggtg
     4501 tatctgcaag tttcttgaaa aagatgaact tggaaaacaa gcgccctcga ctgttcaggg
     4561 atttacctct atttcctaag gagcaattac gtgaaaaata tactggtgga gatattgtga
     4621 ttcatgcttg tgcagatgat gagcaaattg cctttcacgc tattcgcaat ctcatacgta
     4681 aggggagaaa tgcggtgccc cttcgttgga gtcagtctgg atttgcagct atcggggacc
     4741 gaatggagac tccgcggaac ctctttggtt ttaaggatgg aacagcaaat ccaaccaagg
     4801 agcaagactt tgaccgcgtt atctgggctg atagtaagga ctggatggaa aatggttctt
     4861 atatggcagt tcgtcggatt cagatgtttt tagagacctg ggaccgcact ggtctctaag
     4921 aacaagaaaa tacctttggt cgttacaagg aaagtagtgc ccctttcggt aaaaaaatga
     4981 gtttgatgaa gtgaatttga gttttttgct agatgattcg catgttcgtt tggctaagta
     5041 agtggacaag ccacttttgc gttgttctta ctcttactca gatggcattg atgaaaagac
     5101 tggtcagttt gatacgggtt tgctcttcat ttctttccag aaggatcctg ataactttgt
     5161 taaggttcaa actaatctag gtgctactga taagatgaat gaatacatca ctcacatagg
     5221 tagtggactt tttacttgct ttggtggtgt ggagaaagga gactacattg gtcagaaatt
     5281 attggaaagc taggagctgg ttgcttgttt tagctttgtc atttttaatt ctttccccag
     5341 taggtgccca agaaagtttg agttcttatt tcgtaaaaat cacagatact tctaaagcgg
     5401 tcaaaaatgg caatcagtct gaggcccaga aacttgttca agagatggca tcagacttcg
     5461 aaagagtaga aaataaggat tctgaggttg gtaagattgt taaggaaaag ttagctctat
     5521 ctggagatat tactgaagcc aaactcaccg aaatttcctc agctctatta gcttttgaaa
     5581 aagaacaaaa tcctgttgac ctagatgcgg aaaaggaaaa attggtaaac cgtctaagtc
     5641 ctcgttttga aaccttagag caggccattg cctccaaaga tttggaaaaa gtccgagaag
     5701 cttttaagaa aatgaattcc acttggacca tcaatgaaag tgtggttcgt gacaatagta
     5761 tagctcatta tggacgtgtt gaaacagcta tttccttcct gcctagtagc atggaaaccg
     5821 aacctacaga tgagtctgga acatgaaaac agcagttgat ttcaagaaac aaaaaggaac
     5881 agccgaaact gttctcttat cattatttga gaccgtattt tttgttgaaa cgatccacgc
     5941 gtccatctgc ttgagtgaac ttttgacgtc cagtgtagaa tgggtgtgag tctgatgaaa
     6001 tttccacacg gatcaatggg taagtctcgc cttcgaactc aactgtttcg ttagagcgtt
     6061 ttgttgaacc gctaaggaat tggtaaccag tagttgtgtc catgaagaca actgggcgat
     6121 attctggatg gatatctttt ttcattttgc aatatttcct ttctgccatg gtctctttgc
     6181 gagccataga ttgttaccat actagtctat cagattctga aaagtttggc aagtatttta
     6241 tcagttttta agcaagtttt ttaacttttg gtagatgatt tcgttttcct ctaggctata
     6301 ggaattagca ccacttgcta gagggtggcc tccaccatca tgttccttgg caatttcatt
     6361 gataggatgg attttactgc gtaagcgaac gcggtagtgg ccatcagcct gttccacaaa
     6421 aattccccag agactaacac tatcaatacg cccaggtgca cttacaatgg ctgcagtttc
     6481 agcatcggtt acattgtatt gtttcaagac ttcttgactc aggataacgc gagcagcacc
     6541 attttcatcc acttccagat ggtcgtagat gtagccttgc agtttagcaa ttttgtagct
     6601 catagtgtcc attttgcgag tgagagccgc aaagtcaaag ttatgttctc tcaaataagc
     6661 agccaggcga agagtccgtg cagtggtaga agggtagagg aagcgacctg tatcaccgac
     6721 aattcctgca aagagcaact cagcatcgcg atctgccaag gctagttggg ttgtttgggc
     6781 aaatagggta atcatctcgc tagcgctact tgaactagta tcgacccaag acaggtcacc
     6841 gtatacatca tcatttggat ggtggtcaat cttaatgaga aaatcacctt gactatagcg
     6901 tttatcatcg atacgagcag tattagctgt atcacagacg atgacaagtg cgccttggta
     6961 ggccctatct tcaacaagat ccatctcagc catccaagta agagttggtt catcaaaacc
     7021 gacggctttg atggtttttt ctgggaaatg atgttccagc aaggctttca atcccacctg
     7081 acttcccaag gcatcagggt ctggtttcat atgacgatga atgataatgg tgtcgtattc
     7141 tttaattttt tctaaaattt gttggcaaat ctccataaat aacctcaatt ctttctcatt
     7201 tcattgtagc acaagaattt ttatttttaa aatgaaaaag atgtgatata atggagaaag
     7261 ataaattgag gaggacgata tggcattagc aaaaattgta tttgccagta tgaccggtaa
     7321 taccgaagaa attgcagata ttgtagcaga caaattacgt gacttgggct tggatgttga
     7381 tgttgatgaa tgtacaactg ttgacgcttc agacttcttg gaagcagaca tcgctatcgt
     7441 tgcgacctat acttatggtg atggtgaatt gccagatgag atgatggact tctacgaaga
     7501 cctagcagat ctcaacttga atggcaaaat ctacggagtg gtcggctcag gagatacctt
     7561 ctacgatgaa ttctgtaagg ctgttgatga ctttgaccgt gtctttgtgt caacaggagc
     7621 agaaaaaggt tcagagtgtg ttaaagttga tctttctgcc gaggaagaag atattgaacg
     7681 cttggaacaa ttcgcagaag aattggttgc taaagtagga taagcataaa agtgcgctga
     7741 tgtaatcaat cagtgcattt ttcttatcga gagttgggat tttactagcc taattgatgg
     7801 ctttttgata caataattca aataaaggag gagactgtat ggatttagat attattcgtc
     7861 aagaaatcga tcaaatcgac gaccaaattg tcaaactgtt agaagaacga atgcatttag
     7921 ttgagggggt ggttgcttat aagaaagctt caggtaaacc gattttagat actaagagag
     7981 aagaagttat ttttgaaaag gttgggagtc gtgtagagga caagcgctat caggagacta
     8041 ttgtagcgac tttttcggac atactcaaac gttcgcgtga ttatcaggat caaaatatca
     8101 aatgaaaaaa gaacaatttt atccgctagg aatttttcta gctgctatgt tgggcggact
     8161 tgtccgatat ctagtttcca cctggttacc agccagtcca gactttcctt ggggcactct
     8221 ctttgtcaac tatctgggaa ttttctgctt gatttatctt gtcaagggct atctggtcta
     8281 taaggggact agtaagggct tgattttagc actggggacg ggtttttgcg gaggtttaac
     8341 aactttttcc agcctaatgc ttgatactgt gaagctgctt gatacagggc gttatcttag
     8401 tttgatactg tatttgcttt tgagtatcgg tggaggcctg cttttagctt actatttggg
     8461 gaggaagaaa tggtaatcgt ctatcttgca atcgcctgtg gcttgggagc actcgtacgg
     8521 tatttctttt ctcgctataa tcaagtttct aaattgccac tcggaacgct catagctaat
     8581 cttttaggtt gttttttaat tggagtattc tacaatcatg tagagtctaa ggaagtatat
     8641 gctattctag caacaggatt ttgtggcggt ttaacaactt tttcgacctt gaatgacgaa
     8701 cttcaaagac tgctaagtga taagaaggtc ttttattctt acctgacttt gacttacata
     8761 ggtggtttgg ttgcgatttt tttaggaatt ttgctataaa tttctttact tttttttaga
     8821 aatttggtaa actgtatctt gtgtgtaatg gacgcacaaa aatacagttt atccgctgag
     8881 gaagattcct caagattgac aaagatagga gaataaaatg aatccattaa tccaaagctt
     8941 gactgaaggt caacttcgta cagatatccc atcattccgt cctggtgata ctgttcgtgt
     9001 acacgcgaaa gttgtcgaag gtaaccgcga acgtatccag atttttgaag gtgttgttat
     9061 cgcacgtaaa ggtgctggaa tctcagaaaa ctacacagtt cgtaaaatct ctaacggtgt
     9121 aggtgttgag cgtatcttcc caatccacac tccacgtgtt gaaaaaatcg aagttgttcg
     9181 ttacggtaaa gtacgtcgtg cgaaattgta ctacttgcgt gctcttcaag gtaaagcagc
     9241 tcgtatcaaa gaaatccgtc gttaattcga ctaaaaaact ctgcttttca gcagagtttt
     9301 tatctagctc ccttagttca atggatataa caactccctc ctaaggagta gttgcaggtt
     9361 cgattcctgc agggggcagt aattagatat caaagaagtt tgtcaaaaag tctttgatat
     9421 taaaagaacg cataatatag gggattgtct ccataatatt atgcgtttta ttattgaaaa
     9481 atttgggcaa tcatcagata aaattgagac tatatcgagt atataaatgt ttgtatagtc
     9541 aatttattca aaatcgtcaa gttctaaatt atttaaagga ctataacatt catatttctt
     9601 taagtaattg tttatttctt cagcagtgga acgaggtaaa ttaactctta aatagtcaat
     9661 atcttcctta gataaaaaat gatttgcatt caaaattgag aagcgaaata atgcttcatc
     9721 aaaagcaaaa tcataatcgt catcagataa tgattccatt aatgtttgaa tatgttttag
     9781 tgagtagtca ggtatcgaat cgggataata tactggataa tcagtggctg aaaaattgtt
     9841 ttctaatcct gaaatagtaa ttatcaaaga ttctatgtaa tatattaaag catgtttaat
     9901 attaccttct tgttccaaat gtcttccctg agcctttcgt gtgttagata agcatccaaa
     9961 atctcctgat acagtattcc tatgagcttg tttatttaag agactccatt ttatatcacc
    10021 aaaagttgct ttaaaagaga tattgttttt ttctttacaa taatcttcca tacttatgtc
    10081 tttatcatag tatgctttga tatagtcact atgttcgata ataagatttt gtgcttcttt
    10141 tgttagttta aattcgaacg gaatatctaa tttgtcaacg ttgatattat taattattct
    10201 tgtaattaat tctgcttttt ttccagaagt agctaaatta tgtttagata atatagtttt
    10261 taattctact actttaagat atgataaagt cttattttta gtgaagtatc ctaatttgta
    10321 taattgattg tgtgtagatt cagggtcaat ttgaagagag tatttaaaat atcttgggaa
    10381 attataattt ctccctgttt tttgagtcca atctatgagt atgagttgcc caggagtaag
    10441 gccgcactct aatttgttta ggtaaaaatt gttacaatca gaatttgtgt ctctattatg
    10501 attattttgt tttaaattaa atagtttgct aaaaaaattc attggatttt attgctaatc
    10561 aattcttatt gaaaaatgat acttttacag tacccatgta cggatggcat gggcaacgcc
    10621 agattcatca ttagatttag tgatgtattt ggcgattttt ttgatttctg gatttccatt
    10681 ttccatgaca acagggttgc caacgacttc cagcatggca cggtcatttt cttcgtctcc
    10741 gatagccatg gtttcatctt tggttaatcc tagtttttca gctaagtgag taatagctga
    10801 acccttgtct acattctttt taaggagttc gaggtagaaa ggagcagatt tgttgataga
    10861 gtagtgctcg taaaattcgg ctggtatttt ttcaatcgca gcatcgagaa tttctggttc
    10921 atcgataaac atacatttaa caatttcttt gccagccatt tcttcagggg tacggtagaa
    10981 gataggcatg ctgacgaggg ttgattcgtg tacagtgtat gttccgatat tgcgatttgc
    11041 agtatagata ccgtccttgg taatggcatg catgtggaca ccgagcttgc gactgaggaa
    11101 ttccatatcc agataatcct cataggtcaa ggattcgctg ataatctcat ggccagtagc
    11161 agtttcttgg acaagggcac cattgaaggt caccacatag tcaccttggt ctctcaactg
    11221 caagtcgtct agaagtttgg caacacctgc gatggggcgg ccagttgcaa tcacgacttt
    11281 gacaccagct tctttggcat cttggatggc agaaaagact tcaggagtga tttccttttg
    11341 gctgttgaca agggttccgt cgatatcaac ggcaattagt ttaatactca taaatttcct
    11401 cttctagttt ttatttgggg taaaatgatc gttctcaatc aagtataaaa attgctgggt
    11461 aatgctagca aagatactgt tttgatctaa catttctttc ggaaaataga aacgattatc
    11521 tccgtgacga ctaccagcaa gggattggac gataggagac aggctagaga gttcggccag
    11581 ttctccattc ttttgtaaaa tctcaatctg tgtccgtgga ttttcagatt cgggacgata
    11641 gatatcataa gggaggtcaa agttcttatg aatggcagta tagtagtcgg gatcaaagcc
    11701 aatgtcctca accaattttc tcatgctagc gagttggtct tgatcttctt gtgaaaaggt
    11761 aatggattta aagaccttgc ggttgacaaa gcgttgcgat aaatctgcga gaattttgtc
    11821 aggactggtc atccagagct ggaagtaggt attcatcaca ccatcatcta gagccagata
    11881 gtcagacaag gtcacatttt tttcaaagaa aggcaggagg tgtggggaag ttcgtgcaaa
    11941 gaagtctttg tccttagggt agagttcctt agcgcgcttg agaagattct gtaggagaac
    12001 ttccatagcg cgtgttgctg ggtggaaata gacctgcata tacatctggt agcgactgag
    12061 gacatagtct tcgatggcgt gcatgccatt gcgctgaaag gcgataccat tttcgatagg
    12121 acgaatgact cggaggattc gagtcaggtc aaattcccca taggatgctc ctgtaaaata
    12181 ggagtcacgc aagagatagt ccatgcgatc tgcgtcaatc tgactagaaa tgagttgtac
    12241 gacctgcttg ttaggatagg tatggtctat gacactggca actttctctg gaaaatcggg
    12301 cgctacttgt agcaagactt ggtgaatctc tgtctcagga ctttgaataa tctcctgagt
    12361 aatagcttca tggtctgtat caaagagatg ttcaaaagta tgggagtagg cgccatgccc
    12421 aaggtcatgt aggagagcag cgatcatggt caagagagac tcggcaggat tccattcctc
    12481 aggatatttt tcttcgaaaa tctctgtgat gcgtcgtgca atttcataga ctcctagaca
    12541 gtgagagaag cgactgtgtt ctccaccgtg gaaggtataa ctggaagttc ccagttgttt
    12601 gatccggcgc aaacgctgaa attcttttgt attaatcaag tcatagatga tttgattaat
    12661 gacatggatg tagttgtgaa ctgggtcacg gaatactttt tcgttcatat gaccattata
    12721 gcaaaatcct gctctttttg gtaaaatagt aggacaagag acaagaaaga gggcctgatg
    12781 tgaagattcg gatgcgaaat acaattcagt ttgatgagca gttggaagtg attgaccagc
    12841 tttatgatgt ggaagtgcat gaaaaaggag attatagcta cctgcttttc tataatgagg
    12901 aaaaggaaaa agtagtgatt aaatttcatg gtcaagaact tgtgatgagc cgattttcta
    12961 atcccaagac cattatgcgc tttctaaaga atagcgatag tttagcctat attcctacac
    13021 ctatgggcat gcaggagttt atcatccaaa cgagccatta tcaagttgat aggcaaaaga
    13081 ttgaactaga ttatcaacta caaaatcaag agggacatcc ttttgccagc tatcaattgg
    13141 aaattacttg gggctaggct catgtctttt tcaaatttag ctatctatct tcttggatcg
    13201 gtttcctagt gtggtagatt aggctaattt ttggtataat aaaagttatg gaaatcgaaa
    13261 aaaccaatcg tatgaatgcg ctctttgaat tttatgcggc gcttttgaca gataagcaaa
    13321 tgaattatat cgagctctac tacgctgatg attacagcct tgctgaaatt gccgaggagt
    13381 tcggtgtcag tcgtcaggct gtctatgaca atatcaagcg aacagaaaag attctggaag
    13441 attatgagat gaaattgcac atgtactcgg actatattgt ccgcagtcag atttttgatc
    13501 agattttgga gcgctatccc aaggataact ttctgcagga gcagatagaa attttaacaa
    13561 gcattgataa tagagaataa gaggaagaaa aatggcattt gaaagtttaa cagaacgttt
    13621 gcagaacgtc tttaaaaatc tacgtaaaaa aggaaaaatc tctgaatctg atgtccaaga
    13681 ggcaaccaaa gaaattcgct tggccttgct cgaggccgac gttgccttgc ctgttgtaaa
    13741 ggactttatc aagaaagttc gtgagcgtgc agtcgggcat gaggtcattg atacacttaa
    13801 tcctgcgcaa cagattatta aaatcgttga tgaggaactg acagccgttt taggttctga
    13861 tacggcagaa attatcaagt cacctaagat tccaaccatc atcatgatgg ttggtttaca
    13921 aggggctggt aaaacaacct ttgctggtaa attggccaac aaactcaaga aagaagaaaa
    13981 tgctcgtcct ttgatgattg cggcggatat ttatcgtcca gctgccattg accagcttaa
    14041 gaccttggga caacagattg atgtgcctgt ctttgcactt ggaacagaag taccagctgt
    14101 tgagattgta cgtcaaggtt tggagcaagc ccaaactaat cataacgact atgtcttgat
    14161 tgatactgcg ggtcgtttgc agattgatga gctcctcatg aatgagcttc gtgatgtgaa
    14221 agcattggct caaccaaatg aaatcttgct tgtcgttgat gctatgattg gtcaggaagc
    14281 agccaatgtt gcgcgtgagt ttaatgctca gttggaagtg actggggtca tccttaccaa
    14341 gattgatggc gatactcgtg gtggtgctgc tctgtctgtt cgtcacatta ctggaaaacc
    14401 aatcaagttc actggtacag gtgaaaagat tacggacatt gaaaccttcc acccagaccg
    14461 catgtctagc cgtatccttg gtatggggga tatgctcact ttgattgaga aagcttctca
    14521 ggaatacgat gaacaaaaag cccttgaaat ggctgagaag atgcgcgaaa acacctttga
    14581 ttttaatgat ttcatcgatc aattagatca ggtgcaaaat atggggccga tggaagactt
    14641 gctcaagatg attccaggta tggccaacaa tccagccctt caaaacatga aggtggatga
    14701 acgccagatt gctcgtaaac gtgccattgt gtcttcgatg acacctgaag agcgtgaaaa
    14761 cccaaatttg ttaaatccaa gccgtcgccg tcgtattgct gctggttctg gaaatacatt
    14821 cgtcgaagtc aataaattca tcaaggactt taaccaggct aaacagctca tgcagggtgt
    14881 tatgtctggg gatatgaata aaatgatgaa gcaaatgggg attaatccaa ataaccttcc
    14941 taaaaatatg ccaaatatgg gaggaatgga tatgtctgcc cttgaaggaa tgatgggaca
    15001 aggcggtatg cctgacttat cagctctcgg aggagcagga atgccagata tgagccagat
    15061 gtttggtggc ggtttgaaag gtaaaattgg tgaatttgcc atgaaacagt ccatgaaacg
    15121 tatggctaac aaaatgaaga aagcgaagaa gaaacgcaag taagacaaga gattgatgca
    15181 aatctcattt gatgagaaaa taaagtaaaa atttctatga taaaacgtat agtatcaagg
    15241 ttttagaata tctgatatta tacgtttttt gattttaata attttgttaa gacactttta
    15301 ttatggtatg cgaaaaatcc actcagctgt ctgagtggat taggtgtatt tagactctta
    15361 gtctttattt tcgtatggca agatcaagtt caagatgatt cctgtcatgg ctgaaagggc
    15421 agtacctgaa agtgtaactg gaccaagttt aaggatagct cctccaagtc caagaaccaa
    15481 catagcactt gcgatgatga ggtttcgcat ttgagcgaaa tcaacacgtt ctttaatcaa
    15541 gactttcaaa ccattgctgg cgataacccc atagagaagg attgacatac caccaagtac
    15601 agcgtttgga atagttgaaa tcaaggcagt gaatttacca aggaagctga gggcaatcgc
    15661 gatgaaggca gcgttacgga taactgagac agaagcgata cgagtcatac cgataacccc
    15721 tgtattttct ccgtaagttg tattggctgg tccaccaagg aaggcagaaa cagaagttgc
    15781 gataccgtca ccaagaagag tacggtgaag acctggttct tttaagaatt gacgaccaca
    15841 gatttgaccc aaaacagtat ggtctccgat atgttcagaa attgttacga tagcgattgg
    15901 caagatagcg atggcttctg gaccaaagta aagattgtac tctttaaagg caccacctgt
    15961 gctaaatggc aagtagaaac caggaatttc gaaccagttg gctttaagaa ctggtgtaaa
    16021 gtcaaccaag ccaagagtta gtgcgaaaag gtaaccaccg ataatggcaa agaggaatgg
    16081 aatgattcgt aggaagcctt ttccttttgt attgataaag gcagcaatta ggaaagtaac
    16141 aacggctacc agagcatttt tccaatttcc gtctgctaca agacctgcat tggtaacagc
    16201 tgaacctgca agtccaagac cgataacgat gatcatagga ccgataatga ttggtggcaa
    16261 gagtttatca atccattttg ttcctacaaa tcggatgctg gtagcaacaa ggacatagac
    16321 caaaccagtc aagataaccc ctgtttgggc agcagataca tcccccccca tttctttcat
    16381 agccagtgac atagctgtga taaaggcaaa tgaagaacct agataaactg gaactttaaa
    16441 accagtagca atcatgtaga tgagtgttcc aacacctgaa gcaaaaaggg caacagatac
    16501 aggcattccc aaaatcaatg gtaccaagat ggtcgcacca aacatggcga aaacgtgttg
    16561 gaagctaagg agaattcctt ttccatccga aggacgttgg tcaacgtcta gtaacaaatc
    16621 aacagttgat tcttgtttca tataaacctc acttttgggc atcagaaaag agcccattat
    16681 tttacagcaa taggccctca gagtaagact tctctaaaat ctagacttta aagagtttca
    16741 aatatttttg cgtcttcgtc acctcacggg atgacattaa aaacctttgc ttaggatagt
    16801 atagcagaaa aaaatgattt tgtaaatcat tttttcccga gcctagaaat aaaagagcga
    16861 ggctgatttt gtaaacattt gtttactaaa aaatttaaag tgggcgttta tttggcatac
    16921 cagccttacg tggatattta tttggtgttt cttttttctt ttctaccact gtgatatagc
    16981 gcggatctct attcggtagg gcgtagctga gattgtcttc gaccttacta aaaaggagat
    17041 tgagggcatt cttagcttct aataattcct caggcgcatt gctggccttg agtgccaata
    17101 gtttgccacc aaccttaagg tagggaatag tcaattcaga taggacctgc atacgggcaa
    17161 ccgcacgagc tgttacaaaa tcatattgag cacggaagtt cttgtcttgg gcaaaatctt
    17221 cggcacgtcc gtggtagaaa tgaactccgt tcaaatccag ttcttgagcc aagagttgta
    17281 ggaagttgat gcgcttattg agtgaatcaa taatggtcac atctaactcc ggatagagaa
    17341 ttttcattgg tagactagga aatcctgccc cagccccgat atcaagaagt ttgatagttt
    17401 cattgggaat caaaccttga agaatgggtg caatcgaatc gtaaaaatgt ttgagataaa
    17461 cttcttcctt gtccgtaatc gccgtcaaat taatcttctc attccactcg accaagagct
    17521 caaaataacg ttcaaattgt tctttttgct ggttcgaaag tggaagattc tgctcggcaa
    17581 gcaagttgta aaatgtttct ggtttcatag ttatttcctt ctttagtatc atcttatttt
    17641 accacaatca tagaaaattt gatatactgg tactaatcta aaagcagaaa ggacttatat
    17701 tatgacttgg attattcttg gagttatcgc tcttattgtt atttttgtga ttgttagcta
    17761 taacggtttg gttaaaaatc gtatgcaaac caaggaggct tggagtcaga ttgatgttca
    17821 gttgaaacgt cgaaatgacc tcttgccgaa cttgattgag actgtaaaag gttatgccaa
    17881 atatgaaggt tctacccttg aaaaggtggc agaactacgt aaccaagtgg cggcagcgac
    17941 ttcaccagca gaagctatga aagccagtga tgccctcact cgtcaggttt caggtatttt
    18001 tgcagttgca gaaagctatc cagatttgaa agctagtgct aactttgtta aattgcaaga
    18061 ggagttgaca aacacagaaa ataaaatttc ttactctcgt caactctata acagtgttgt
    18121 cagcaactac aatgtaaaat tagaaacttt cccgagcaat attatcgctg gaatgtttgg
    18181 atttaaagcg gcagatttcc ttcaaacacc tgaagaggaa aagtcggttc ctaaagttga
    18241 ttttagcggt ttaggtgact aagatgttgt ttgatcaaat tgcaagcaat aaacgaaaaa
    18301 cctggatttt gttgctggta tttttcctac tcttagctct tgttggttat gcggttggtt
    18361 atctctttat aagatctgga cttggtggtt tggttatcgc actgattatc ggctttatct
    18421 acgctttgtc tatgattttt caatcgacag agattgtcat gtccatgaat ggagcgcgtg
    18481 aggtggatga gcaaacggca ccagacctct accatgtagt ggaagatatg gctctggtcg
    18541 ctcagattcc tatgccccgt gttttcatca ttgatgatcc agccttaaat gcctttgcga
    18601 caggttctaa tcctcaaaat gcggctgttg ctgcgacttc aggtctacta gctatcatga
    18661 atcgtgaaga actagaagct gttatgggac atgaagtcag tcatattcgt aattatgata
    18721 tccgtatttc gactattgca gttgcccttg ctagtgctat caccatgctt tctggtatgg
    18781 caggtcgtat gatgtggtgg ggtggagcag gtcgcagacg aagtgatgat gaccgagatg
    18841 gaaatggtct tgaaatcatt atgctagtgg tttccctact agctattgta ctggcacctc
    18901 tcgctgcaac cttggttcag ctcgctattt ctcgtcagag ggaatttctg gcagatgcat
    18961 ctagtgttga gctgactcgc aatccccagg gaatgattaa tgccctagat aagttggaca
    19021 atagcaaacc gatgagtcgc cacgtcgatg atgctagcag tgctctttat atcaatgatc
    19081 ccaagaaagg tggggggttc caaaaactct tttataccca cccacctatc tcagaacgga
    19141 ttgaacgttt aaaacagatg taaaagaatc cagaggtcgt ctctggattt ttgctatgtt
    19201 gcaaatttat aaatcttcta aattaactaa ctccaaaccg tgttctgtca tgcgatagat
    19261 gatcgagcag acttctttta agaaacgacg gtcatgcgaa acagtgatga gaccgcctgg
    19321 ataggttgca aagagttttc tgatttgggg ttgagaagtg ggagaaaagt ttcgtgtggg
    19381 ttcatccagc aggagaaagt ttggtttgcg caggactaga tccaaaagca ggagttttcc
    19441 ctgttgtccg ccagataagg agcgaatttg atgctgcatt tctggataac tgaaattgag
    19501 actggctagg tgagagtgga ttttctgaag ttcctctttt tccccagttt tgctgatata
    19561 agctattggt gataaatcca attgtagttt tttgtggtaa tcttgtggca taaaaccaag
    19621 ggaaatctct cttttatcat tcagaagttg ctttaatttg gctaacagag ttgatttccc
    19681 aacaccattt ggcccgataa taccgatttt ttcttgccca cggacagtta gttgtagttt
    19741 ttgaaccaaa actcggtcgt caatggacaa attttctttt tccagttgga ctaagacttt
    19801 agaagctggt aatggttgga tgtctgaaaa gaaaagttgg atttgttcct cttcaagtgg
    19861 tttttgagtc atggactgag ctgccttttc gtagcgtttt tcttgtgaga ggacagtttt
    19921 catcttttta gccaataggc gaccggcagt actatctttg gtagctcgaa gcgcagtttc
    19981 tacattttgc ttaactctcc gatgtttttc catggtttta tcgtaggctc tttggttgtt
    20041 agcagcttgc tgactttgtt tggcaaaatt agcctttctc tgctcactat agctatcata
    20101 gtctaaatgc tctactagcg tttccgcttc tttacggtgt ttgaccagtc gcaagtgaac
    20161 aatagtgtct gccgtttcag aaagaaagtc ttcatcatgg gaaatgaaaa taacggtttg
    20221 cctggtcttt tgaatctggc cttttagcca atcaactgtc tcaaggtcta ggtcatttga
    20281 aggttcatct aaaaatagaa tctcaaaggg tttggctaac tcatggataa gctgaatttt
    20341 caaagcttct ccccctgata gattgccaat ctcttggtca cttgcgaaac gattgctatc
    20401 aaaatgcaat tcctccgcca aacgatagag gatactgtag tctaaatcaa tagaatctaa
    20461 aaagaagtag tcgtgtaaag ttttcttttt cagctcctcg ggaagttttt gaggaatgta
    20521 ggccagtgac tgatagtcag attggatgtt tcccttgata gtgaaatcag acaaagcttc
    20581 ccccattaaa gttttaagta aggttgattt accatttcct tcttcgccaa taatagctac
    20641 cttttccccg tcttgtatgg tcatgcttag gtcagagaca aggtctcgta aatctttgtt
    20701 ttgtgtgatg gtcaggtgat tgatttttat catactattg ccctttctag aatgttcata
    20761 gtgcataaca aaaagctcta ctttacctag tagagcccaa agtctatgtc aaaaaaactc
    20821 tattaacatg ccagaaaaag ggcataaaag agcacacaaa aagagacaaa aacaagatct
    20881 actaaataaa ggttctttat ttgatagact tagttgttca tgtctccctt acctcctagt
    20941 attagtatct ttatactata cttttaagga aattttgtca acagaaaact ggaaattcta
    21001 tgctctaaaa tccaaaaaca ggtctataga gacttagtac gtgtttgaca tgctcgtaat
    21061 ggattttatc atagtttcgc caagcagtgc gttcttgatt gtttagtttt aggctaccca
    21121 gtccaatcat aggactggtg ggttggtaaa atttctcaag atcaattaag atactgttgt
    21181 tcaacttttc cgttttttga gttcttgcat agtagtgttt agcagctctt gtagtcgaat
    21241 atcatctgcc gtaccctgtt tacagttttg gtagctttta tttaacttgg ataggagttg
    21301 aatgagtttc ttgtcttccg tgtgagcatc ctccttgatc tgatgtactc tttccctata
    21361 gtatagcact aaaaaataaa actgtcatag tgaatatgtt aaaatttcga attttaatta
    21421 caaagtttgg tgtgctgatt tgtagccttt tcatggtata atcaagagag taaatcttta
    21481 tggaggaagt atgaaattaa atattcaaga aattcgtaag cagtctgaag gtttgaactt
    21541 tgaacaaacg ttagatttag ttgatgacct gcgtgcacgt aatcaagaaa ttttagatgt
    21601 aaaagatatc cttgcagttg ggaaagtaca atatgaagac cgtatgtatt tcttagatta
    21661 tcaactatct tataccattg ttcttgcttc gagtcgcagt atggagccag ttgagttagt
    21721 tgaatcttat ccagtcacgg aagttttcat ggaaggcgca actaaccagc tagatcaaga
    21781 agttttagat gatgacttgg tcttgcccat cgaaaatggg gagcttgacc ttgctgagag
    21841 tgtatcagac aatatcctgc taaacattcc tatcaaggtc ttgacggctg aagaagaagc
    21901 tggtcaagga tttgtctcag gaaatgactg gcaaatcatg acagaggaag aataccaagc
    21961 tcaacaagca gtaaagaaag aagaaaacag tccttttgct ggcttacaag gactatttga
    22021 cggagatgaa taatggtctt gtcaaaaaaa cgagcacgaa aggtgctaga agaaatcatt
    22081 gccctcttcc cagatgccaa gcctagtctt gattttacca atcattttga actcctggtt
    22141 gcggtcatgt tatcagccca gacgacagat gcagcggtaa ataaggccac accaggtctc
    22201 tttgttgcct ttccaacacc ccaagctatg tctgtagcga cagagagtga gattgcttca
    22261 catatttctc gcttgggatt gtatcggaat aaagctaaat tccttaaaaa atgtgcccaa
    22321 cagttactag acgattttga tggtcaagtc cctcagacac gtgaagaatt ggagagtttg
    22381 gcaggtgttg gtcgcaagac agccaatgtt gtcatgagtg taggatttgg gattccagcc
    22441 tttgcagtgg atactcatgt ggagcgtatt tgcaaacacc acgatattgt caaaaaatca
    22501 gcgacgccac ttgaggtgga aaagcgggtc atggatatct tgccgcctga gcagtggtta
    22561 gctgcccatc aggccatgat ttattttgga agagccattt gccatccaaa aaatccagag
    22621 tgtgaccagt acccacaatt atatgatttt agcaatttgt aagatgctac attgaaactt
    22681 tttgcttgat tttaagcatg ctttgtgcta tagtaaatga taactaaata ttcctttaaa
    22741 cctgtcccgt gaggcaggca aggagcgcga taaagtagaa gggtgaagtc tatactgtgt
    22801 gcagtagggc tcgcttggct ataaatttca agtctccttg tttaaggaga cttttctttt
    22861 tggaggtttg tcatgaaaac aaaagaagtt gtagacgaat tgaccgtcaa acgagcgatt
    22921 acgcgtatta cttatgagat tatcgaacgc aacaaagatt tgaataagat tgtcttggct
    22981 ggtattaaaa ctcgtggtgt ctttattgcc caccgaatcc aagaacgttt gaagcagtta
    23041 gaaaatcttt cagttcctgt tgtggaattg gatactaaac ctttccgtga tgatgttaaa
    23101 agtggagaag atacttcttt ggtttctgtc gatgtgacag accgcgaagt tatcttggtg
    23161 gatgatgtgc tctatacagg tcgtaccatc cgtgctgcta ttgataatat tgtcggtcat
    23221 ggtcgtcctg cgcgtgtgag tttagcagtt ctagtcgatc gtggacatag agaattgcca
    23281 atccgtccag attacgttgg aaaaaatatc ccaaccagtc gttctgaaga aatcatcgta
    23341 gagatgacag aacttgatga ccaagacaga gttctgatta ctgaagaagc ttagaaagtt
    23401 aaaggagtag ccatgtcaga aaatcaacaa gcattgaacc atgtggtgtc catggaagac
    23461 ctcactgtcg atcaagtaat gaaattgatc aagcgaggaa ttgagtttaa aaatggagcc
    23521 cagcttccct atgaagacca tccgattgtt tccaatctct tctttgagga ttctacacgg
    23581 acacataagt cctttgaagt cgcagagatt aaacttggat tggaacgact tgactttgat
    23641 gtgaagacta gttcggttaa taagggtgag acactttatg acaccatttt gactctgtct
    23701 gctttaggag tggatgtctg tgtgattcgc catccagagg tcgactacta tagagaattg
    23761 attgctagtc caacgattac gacttccatc atcaatggtg gagatggttc gggccaacac
    23821 cctagccaga gcttgcttga tttgatgacc atttatgagg aatttggcca ctttgagggt
    23881 cttaaagttg ctattgcagg tgacttggac cactcacgcg ttgccaaatc caatatgcag
    23941 attttgaaac gcttgggatc tgaactcttt ttcgctggac ctgaggaatg gagaagtcaa
    24001 gagtttgcag actatggaca gtttgtaacc attgatgaaa tcattgatca ggttgatgtc
    24061 atgatgtttc tccgtgtgca acacgaacgc catgatagtg gagctgtatt ttcaaaagaa
    24121 gactaccatg cccaacatgg cttgactcaa gaacgttacg atcgtttgaa agaaacagca
    24181 atcctcatgc acccagctcc aatcaatcgt gatgtagaaa tagcagacca cttggttgaa
    24241 gcaccaaaat cacggattgt ccaacaaatg accaatggtg tctttgttcg aatggcaatc
    24301 ttagaatccg tactagcgag tagaaacgcc aactaaaaca caagacgtta aaagacgcca
    24361 gtgagcgtcc acgagaggtg aaaatatgac aaaaagaatt ctagtattag aagatggcac
    24421 agtttttgaa ggcaaggcct tcggagcaga tattgatgta acaggcgaaa tcgtctttaa
    24481 tacagggatg accggctacc aagaatccat tacagaccag tcttataatg gacaaatctt
    24541 gacctttact tatcctttgg tcggaaacta tggaattaac cgtgatgatt acgaatctat
    24601 tattccaact tgtaagggag ttgttgtttt cgaagaagcg cgtagagcaa gcaactggcg
    24661 caaccaaatg accttggatg aatttttgaa agccaagaaa attcctggta tttcagggat
    24721 tgatacgcgt gcacttacca agattattcg taagcatggt actatgcgag caaccttgac
    24781 tcatgttgga gatagtatgg accatgtgac tgaccagctc caagcaacag tattgccgac
    24841 agataatatt aagcaggttt ctactaaaac ttcctatcca gctccaggag ttggtttgag
    24901 cgtggtgtta gtagactttg gtctcaagca ctcaatccta cgtgaacttt ctaagcgcaa
    24961 ctgtaatgtg acggttgttc cttattcgac aacggctgaa gaaattctcc atctcaatcc
    25021 tgacggagtg atgttgtcaa acggtccagg taacccagaa gatgttccac aggcactgga
    25081 tatgattcgc ggtgtacaag gaaaaattcc aatctttggt atctgtatgg gacatcaact
    25141 ctttgctatg gcaaacgggg ctaagaccta taagatgaag tttggccacc gtggattcaa
    25201 ccatgcggta cgtgaaatcg caacaggacg agtagacttc accagccaga accatggtta
    25261 tgcagtcagc cgcgaggatt tgccagagca cttgattatt acccacgaag aaatcaatga
    25321 caagtcagtg gaaggtgtgc gccacagata ccaacctgct ttctctgttc aataccaccc
    25381 agatgcagct cctgggccac acgacgctag ctacctattt gacgagttta tcgagatgat
    25441 ggaagttttt aaacaatcaa actaagaacg agtaaaagct cgtcggagaa tttatgtaac
    25501 tgaacacgga cggaaagctc agaatttaga gaaaccaact tggattgtca atccttcctt
    25561 gatttcttaa aattcaatcg ctttctattc gtcctttgta tcttatttaa tgtcgccctg
    25621 agaggcgacg aatagaaagg agaagggtgc tccgtatttg ctgaactgaa tacgggctac
    25681 ggacggtgct aaaaagatag ttattcctag aactgacagt tcttcgtcat aactcctatt
    25741 ttagctttgt ccgcttgacg cccttaatat cttatatatg cctaaacgta ctgatattca
    25801 aaaaattatg gtgattggtt ctggtccgat tattattggt caggctgctg agtttgacta
    25861 tgctgggacc caggcttgct tgtcgttgaa agaggaaggt tatgaggttg ttttggttaa
    25921 ctcaaatcct gcaaccatca tgacggacaa ggaaattgct gacaaggttt acattgaacc
    25981 gattacactt gagtttgtga cacgtattct tcgtaaggaa cgtccagatg ccttgctacc
    26041 aacactcggt ggtcagacag gtctcaacat ggccatggaa ttgtctaaaa atggtatctt
    26101 agatgagctt agcgttgaac ttctgggtac taaattgtct gccattgacc aagcggagga
    26161 ccgtgacctc tttaaacaat tgatggaaga gcttgagcaa cccattccag aatctgaaat
    26221 tgttaacaca gtagaagaag cagttgcctt tgcagcgaca attggctacc cagtcatcgt
    26281 tcgtccagcc tttacccttg gtggtactgg tggtggtatg tgtgccaacg agaaagaatt
    26341 gcgtgaaatc actgaaaatg gtttgaaatt gtcacctgtt acccaatgtt tgattgagcg
    26401 ttcgattgct ggtttcaagg aaattgaata cgaagtgatg cgtgactcag ctgacaatgc
    26461 cttggttgtt tgtaacatgg aaaactttga cccagttggg attcacacgg gggattccat
    26521 tgtatttgcc cctgcgcaaa ccatgtcaga ctatgaaaac caaatgcttc gtgatgcgag
    26581 cttgagcatt attcgtgccc tcaagattga aggtggatgt aatgttcagc tagctcttga
    26641 tcctaacagc ttcaagtatt atgttatcga agtaaaccct cgtgtatcac gttcgtcagc
    26701 ccttgcttct aaagcgacag gatatccgat tgctaagttg gctgccaaga ttgcagtagg
    26761 tttgaccttg gatgaggtta taaacccagt tacaggttca acctatgcca tgtttgaacc
    26821 agcccttgac tacgtagttg ctaagattcc acgtttccca tttgacaagt ttgaaaaggg
    26881 tgaacgccgt ctcggtacac aaatgaaggc gactggagaa gtcatggcaa tcggtcgtaa
    26941 catcgaggaa tctcttctca aggcctgccg ctcccttgaa attggggtgc accacaatga
    27001 aatacctgaa cttgcagcag tttctgatga tgccttgatt gaaaaggttg tcaaagccca
    27061 agatgaccgt ctcttctacg tatcagaagc aattcgtcgt ggctacacac ctgaagaaat
    27121 tgcagagctt acaaaaattg atatcttcta tcttgataaa ctcttgcata tctttgaaat
    27181 tgagcaagaa ttgggtgctc atccacaaga tctagaagtt ttgaaaacag ccaaacttaa
    27241 tggtttttca gaccgtaaga ttgctgaact ctggggaacg actgatgata aagttcgcca
    27301 acttcgcttg gaaaacaaga ttgttccagt ttataagatg gtagacactt gtgcggcaga
    27361 gttcgactcc gaaacgccat atttctattc aacctatggt tgggaaaatg aatctatcag
    27421 atctgataaa gaatctgtac ttgtcctagg ttcaggtcca atccgtatcg gtcaaggggt
    27481 tgagtttgac tacgcaaccg ttcactctgt taaggctatc caggcagctg gttatgaagc
    27541 tatcatcatg aactcaaacc cagagaccgt ttcaacagac ttctctgtat cagataagct
    27601 ttactttgag ccattgacat tcgaagatgt tatgaacgtc attgacttgg aacaaccaaa
    27661 gggagttatc gttcagttcg gtggtcaaac agccatcaac cttgcggagc cattggcaaa
    27721 agcaggtgtg accatccttg gtacacaggt tgctgaccta gaccgtgccg aagaccgtga
    27781 cctcttcgag caagctctca aagaattgga tattccacag ccaccaggac aaacggctac
    27841 caatgaagaa gaagcagctc ttgcagctcg taagattggc ttcccagttc ttgtccgccc
    27901 atcttatgta cttggtggac gtgccatgga aatcgttgaa aacgaagaag accttcgttc
    27961 ttacatgcga actgctgtta aggctagtcc agatcaccca gttcttgtcg actcttatat
    28021 cgttgggcaa gagtgcgaag ttgatgccat ttcagatggt aaagatgtgc tcattcctgg
    28081 tatcatggag catatcgaac gtgctggtgt ccactcaggt gactcaatgg ctgtttaccc
    28141 accacaaacc ttgtcgcaaa aggttcagga gaccatcgca gactacacca aacgcttagc
    28201 aatcggtctt cactgccttg gaatgatgaa catccagttt gttatcaagg atgaaaaagt
    28261 ctacgttatt gaggtcaatc cacgtgccag ccgtactgtt ccattcctat ctaaggtaac
    28321 caatattcct atggctcaag tagcgaccaa gctcattctt ggtcaaagtt tatcagaact
    28381 tggctaccaa aatggacttt accctgaaag cacccgtgtt catatcaagg cacctgtctt
    28441 ctcctttacc aaactagcta aggtagacag cttgctcggt cctgaaatga agtcaacagg
    28501 tgaagttatg ggttctgatg ctactttgga aaaagctctc tataaagcct ttgaagcttc
    28561 ttatctacac ttgccaactt ttggtaatgt cgtctttacc atcgctgatg atgccaaaga
    28621 agaagccttg aacttggctc gtcgtttcca aaacattggt tatggaatcc ttgcgacaga
    28681 agggacagca gccttctttg ctagtcatgg cttgcaagcc caacctgttg gtaagattgg
    28741 tgatgacgat aaggatatcc caagttttgt acgcaaagga agaattcaag ctatcattaa
    28801 cacagtagga acaaaacgaa ctgctgacga agatggtgag caaattcgtc gttcagccat
    28861 tgaacacggg gtgcccctct tcacagcgct agatacagct aatgccatgc ttaaggtact
    28921 tgaaagtcgt agctttgtta cagaagcgat ttaaatagaa aaataagata tagcagactg
    28981 aatttagaac agtacacagg aatactaaaa tatttctata aattaatttg aattttctaa
    29041 ttgatttgtt cgcatcttat ttcaatctac tatagaaaaa agtctttaaa attataaaac
    29101 gcatcatatc aaggtttttc aaaaaccttg atatgatgcg ttttattgtg ggaatatgta
    29161 tttcattttc tactaaaatt atgtttttga ataacctcta tcttagtagt ttgtataatc
    29221 cccctcaatc agtttttgcg ataagcttta atgctatgac tataccactc ttgcatttct
    29281 tttgatggtg gtgtcatata atcgccatac atctgggtta aaaattggtc atattttttg
    29341 ggaacaggca acatacggcc ctcaaactca gttaaaatca gttctttaaa ggtatcaact
    29401 gggaagattt ctttcatccc ttccttaccg atcccaactc ctccttcata ttgaggaatg
    29461 ttggttacag catttttgac tagttgatca attttcttgt aaaagtagcg aggattgaca
    29521 aatcggagag cgtaccagct acataatcta agaaaatctt ttagtttgct atcaccgtga
    29581 actgctcgtg attttttgat ataagctagt tgacgaagag ccacatactt atagctcttg
    29641 tcgacaatgc tcaagtctgt aaatcgatca attgggaaga catcgatgaa aaggctggta
    29701 tcatgacgct tgtacttaac atggtcttct ataacagtag aagtgtccaa aatcgatgcg
    29761 aaattatgga agtaccaaga agatgtatcg taggaaagaa ccttgtagcg agggtgattt
    29821 tcttcttcaa taatcttcag taaacgctca taatcctcac gataaaggga aatatcaata
    29881 tcatcatccc aaggaatcat acctttgtgg cggatggctc caagcatggt tccataactg
    29941 agaaaataag gaatatcatg tttcttacaa gtctcatcaa tatagtccag cagggctagt
    30001 tgaatttctt taatttcttt tttttctaaa tattgcatcc taatcctcca atttataagc
    30061 gtgaaattca tgactgtaga agcgtttttc ttctggtggt agggtcatat aatctccata
    30121 aaattgtgtc aaaatagtat caaatttttc aggtgcagga aggcttaaat tctcaaaggg
    30181 taaatcgatt gttttatcaa aggtaccact tgggaagact tccttttcct taaattttga
    30241 agggataaaa gccatatact gcccattttc acgactatat ttttgaattt ctttctcgat
    30301 tttatttgca aaataacgag gagaaactgg tcgaaggagc aaccaaaagg ctgttcgtat
    30361 ccaatctttt aaaaggctat ccttatagac aatattttta tgtttactga aagacagcag
    30421 tttgaagctt tccagtttat aacaagtatc aatgacctta ggatcatcaa agcgatctat
    30481 agggaaaata tcgataaaga taccagactc ataggttttt gtatttcgag tatcaataat
    30541 tttagtcgta ctgtcggtta tcttgataaa gttgttaaag tagttcttat cagtttctaa
    30601 ggataggagc ttatacttgc ttttttcctt ttgaaaaatg ttaataaatc gttggtagtc
    30661 ttctctaggc atggataaat caatatcgtc gtcccaaggg ataaagccct catgtcgaac
    30721 cgccccaatc agagtaccgt agttaataat atagttgata ttgtgctttt tacagagagt
    30781 atcaatataa tccaaaattt ctaattcaat ttgtttggca tcttcaatgg ttagttgttt
    30841 cattttaaac tcctatgatt ttttgaattt attttttaag gctaggacat ggtttaaaaa
    30901 ttcatagaaa atgctatctt ttgtgaagac aagtagacta atataagaga tagctgatag
    30961 caagacaatc aaaccagtat taattaaaaa tggcaaatta atgaccatat ccacaggata
    31021 cacgaaatta atcaggaaat aaattgctac aaaggaaagt gaaaagagag agtatcgaac
    31081 agtatagcta aagatatgtc ccaagtggat gagttgtttt ctatggatga aaataatata
    31141 gaaaacaagt agagaagtct ctgataacat agttgtcagt aagtagtatt caggaaccac
    31201 gatatggttg aaaaagagga gactattcaa gcccaaattg agtagcccag caaagactgt
    31261 atagactgtg atacgttttt catagccatt ggtaaagaga atttgggaac caagaatggt
    31321 atctaaggcc aggataatcg tacgaaaagc gaagagagag gtcaagatgc cgcctccgat
    31381 atatttttca ctaccgtaaa gtaggatggc atttggtcct aaaaccatga gtccaaaact
    31441 cagtggaatg ataaagaagt taaagattcg actacctcta ttaaccagag aaacataggc
    31501 ttctttgtct cctttcccca gatagtaact gagacgaggc acactcactc caattgcacc
    31561 tgttacaacc ccagctataa cggtcacaat tcgctgagct atggtatagt aactaacgtt
    31621 gacatcaatc cctgttttaa cgaggaagag gcgatctaaa aaagtgaaga gcatattggc
    31681 attggcaaag actaacatgg ctgtcagagg gagaaagagt ggtttaaaat cacttaggtg
    31741 aatcttaaca agtttgatgt ctcttttaat ccaaaaataa ctaatcaggt agttaatcag
    31801 cgtcgataaa ctcatcacaa gtgtatagac aacaatatcg tgttcatttt taacaaataa
    31861 gaaaatagag accagcatca ggatacggat gaaggcagtt ttgtaaaaga gaaaactgta
    31921 attttccaga gcttcattga cccattcgat tgaaaaaatc tgggcaatga gttgaatccc
    31981 cataacaagg tagacctttt tgacgattgg attatcaata aagaagagag gataggctag
    32041 gatatagaca gcagtggtca aaatcgtaca agcgatgcac aaataaaaaa gactagaaaa
    32101 ggttctgtta agatcttttt tgttatcctt gacattactg atagccctta aaccgtagtt
    32161 atagacacca taagttgcaa agggcaagaa aaatgacaaa atagtgtcga ctgagttgaa
    32221 gtaaccatag tcagttcggt ccaagacacg cgcgacatag gttccagtta ggatgggaaa
    32281 aataatattc aagacacgaa ttcccatgta agatagagca tttaatttta tacttttcat
    32341 tcaatttacc tcgtttttca ttatatcata aagttagcta ataagaaatg aagggcagta
    32401 agtcaagtaa tcactttgaa gtttcaaatc ttaagtttta agttttcttt aaggaaagta
    32461 tattattctg aaggactcta aaatttcgca gccatttatt agtaattgct acagaattcc
    32521 tagtcattac tagaaatgga ctagtttctt tgaataatag aactgcataa ttctcctatt
    32581 ctagaagggg aggaccagta tttcttttat gataggacta gattgtggta taatagagag
    32641 aataagtttt tttagtaaga caaaggagaa aatagatgat ttatgcagga attcttgccg
    32701 gtggaactgg cacacgcatg gggatcagta acttgccaaa acaattttta gagctaggtg
    32761 atcgacctat tttgattcat acaattgaaa aatttgtctt ggagccaagt attgaaaaaa
    32821 ttgtagttgg tgttcatgga gactgggttt ctcatgcaga agatcttgta gataaatatc
    32881 ttcctcttta taaggaacgt atcatcatta caaagggtgg tgctgaccgc aatacaagta
    32941 ttaagaaaat cattgaagcc attgatgctt atcgtccgct tactccagag gatatcgttg
    33001 ttacccacga ttctgttcgt ccatttatta cacttcgcat gattcaggac aatatccaac
    33061 ttgcccaaaa tcatgacgca gtggacacag tggtagaagc agttgatact atcgttgaaa
    33121 gtaccaatgg tcaatttatt acagatattc caaatcgtgc tcacctttat caaggacaaa
    33181 cacctcaaac attccgttgc aaggacttca tggaccttta tggatctctt tctgatgaag
    33241 agaaggaaat cttgacagat gcatgtaaaa tctttgtgat caaaggaaaa gatgtggcct
    33301 tggccaaagg tgaatactca aatctgaaga ttacaaccgt aacagatttg aagattgcaa
    33361 aaagtatgat tgagaaagac tagtaaaatg attaatcaaa tttatcaact aactaagcct
    33421 aagtttatca atgtcaaata tcaggaagag gctattgacc aagagaatca tatccttatc
    33481 cgtcccaact acatggctgt ctgtcatgcg gatcagcgtt actatcaggg aaaacgtgat
    33541 cccaagattt tgaataaaaa gcttccaatg gcaatgattc acgagtcatg tggaaccgtc
    33601 atttctgacc cgaccggaac ctacgaggtt ggtcaaaaag ttgtcatgat tcccaatcag
    33661 tctcctatgc agagtgatga agaattctat gaaaactaca tgacagggac ccatttcttg
    33721 tctagtggat ttgatggctt tatgagagag tttgtttctc tccctaaaga tcgtgtggtg
    33781 gcttatgatg ctattgaaga tacggttgca gccattacag agtttgtcag tgtgggcatg
    33841 cacgctatga atcgtctatt gactcttgct catagcaagc gggagcggat cgccgttatt
    33901 ggagatggaa gtttagcttt tgtggttgcc aatattatca actatacttt gccagaatca
    33961 gagattgtgg ttattggtcg tcattgggaa aagttggaac tcttctcatt tgccaaagaa
    34021 tgctatatta cggataatat tcctgaagag ttggcctttg accatgcttt tgaatgttgt
    34081 ggtggtgatg gtactggacc agctattaat gacttgattc gctacattcg tcctcaggga
    34141 acgattctca tgatgggagt tagcgaatat aaagtcaatc tcaatactcg cgatgcctta
    34201 gaaaagggct tgattttggt tgggtcatct cgttctggtc gcattgattt taaaaatgct
    34261 atccaaatga tggaagtcaa gaaatttgcc aatcgtctta aaaatatcct ttatctagaa
    34321 gaacctgtaa gagaaattaa agatattcat cgtgtctttg caaccgattt aaacacagcc
    34381 tttaaaacag tgtttaagtg ggaagtataa gtactggagg ttaattgtgg agaaaatcat
    34441 taaagaaaaa atttcttcct tacttagtca agaagaggaa gtcctcagtg ttgaacaact
    34501 gggtggaatg accaatcaaa actatttggc caaaacaaca aataagcaat acattgttaa
    34561 attctttggt aaagggacag aaaagcttat caatcgacaa gatgaaaagt acaatcttga
    34621 actactaaag gatttaggct tagatgtaaa aaattatctt tttgatattg aagctggtat
    34681 caaagtaaat gagtatatcg aatctgcgat tacgcttgat tcaacgtcaa tcaagaccaa
    34741 gttcgacaaa attgctccaa tattacaaac tattcatacg tctgctaagg aattaagagg
    34801 agaatttgct ccttttgaag aaatcaaaaa atacgaatcc ttgattgaag aacaaattcc
    34861 ttatgccaac tatgaatctg ttagaaatgc agtcttctcc ttagagaaaa gactggctga
    34921 cttaggtgtt gacagaaaat cttgtcatat cgatttggtg cctgaaaact ttatcgaatc
    34981 acctcaagga cgactttatt tgattgactg ggaatattca tcaatgaatg atccaatgtg
    35041 ggatttggct gccctctttt tagagtctga attcacttcc caagaggaag aaactttctt
    35101 atctcactat gagagtgacc aaacaccggt ttctcatgaa aagattgcta tttataaaat
    35161 tttacaagat actatttgga gtctatggac tgtctataag gaagagcaag gtgaagattt
    35221 tggtgactat ggtgtgaatc gttaccaaag agctgttaaa ggtttggctt cttatggagg
    35281 ttcagatgaa aagtaaaaac ggagttcctt ttggccttct ctcaggtatt ttctggggct
    35341 tgggtctaac ggttagtgct tatatctttt cgatttttac agatttgtca ccctttgtgg
    35401 tggctgcagc tcatgatttt ttgagcatct ttatcttact agcttttctc ttggtaaaag
    35461 aagggaaagt tcgcctctca attttcttaa atattcgcaa tgtcagtgtt atcatcggag
    35521 ccttgctagc aggccctatc ggtatgcagg ccaatcttta tgcagttaag tatatcggaa
    35581 gttctttagc ttcatctgta tcggctattt accctgcgat ttcagttcta ttggctttct
    35641 tctttttgaa gcacaagatt tcgaaaaata ctgtatttgg gattgtcttg attattggag
    35701 ggattattgc ccagacctat aaggttgaac aggttaattc cttctacatt gggattctct
    35761 gtgctttggt ttgtgctatt gcatggggaa gtgagagtgt tcttagctcc tttgctatgg
    35821 aaagtgaact gagtgaaatc gaagccctct taatccgtca agtgacttcg ttcttgtcct
    35881 atcttgtgat tgtgctcttc tctcatcagt catttactgc agtagccaat ggacaattgc
    35941 taggtctcat gattgttttt gcagcctttg atatgatttc ctacttggct tattatatcg
    36001 ctatcaatcg cttgcaacca gccaaggcta caggcttgaa cgtgagctat gtagtatgga
    36061 cggtcttgtt tgcagttgtt tttttgggtg caccgctaga tatgctgacc attatgacgt
    36121 cacttgtcgt cattgctgga gtttatatta ttattaaaga ataaaggaga ttcgtgtgaa
    36181 agccattatc ttagcagcgg gattgggaac tcgcttgcgt cctatgactg aaaatacccc
    36241 taaagccttg gttcaggtta atcaaaaacc tttgattgaa taccaaattg agtttctcaa
    36301 agaaaaagga atcaatgaca tcatcatcat tgttggttat cttaaagaac aattcgatta
    36361 cttaaaagaa aaatacggtg ttcgcctcgt tttcaatgat aaatacgctg actacaataa
    36421 cttttactct ctctatcttg taaaagaaga attggccaac agctatgtta ttgatgctga
    36481 caattatctc tttaaaaata tgttccgcaa tgatttgaca cgttcgactt attttagtgt
    36541 ttatcgtgaa gattgtacca acgaatggtt cttggtctat ggagatgact acaaggttca
    36601 agacattatt gttgatagca aggcaggtcg catccttagt ggtgtatcct tctgggatgc
    36661 tccaactgca gaaaagattg tcagctttat cgacaaggct tatgcaagtg gtgaatttgt
    36721 tgatctctat tgggacaata tggttaagga taatatcaaa gagctagatg tctatgttga
    36781 agaattagaa ggcaatagca tttatgagat cgatagtgtc caagactatc gtaaattaga
    36841 agaaattctt aaaaacgaaa attaaagatt ccaacatctg acaaaatagt cggatgtttt
    36901 ttgatttttt acgaactttt acgaatagat agatgagtag aaaaagaaat ggagttattt
    36961 atgaaaatca caaactatga aatctataag ttaaaaaaat caggtttgac caatcaacag
    37021 attttgaaag tgctagaata cggtgaaaat gttgatcagg agcttttgtt gggtgatatt
    37081 gcagatatat caggttgccg taatccagcc gtttttatgg aacgttattt tcagatagac
    37141 gatgcgcatt tgtcgaaaga gtttcaaaaa tttccatctt tctctatttt agatgactgt
    37201 tatccttggg atttgagtga aatatatgat gcgcctgtac ttttatttta caagggaaat
    37261 cttgacctcc tgaaattccc gaaggtagcg gtcgtgggca gtcgtgcttg tagcaaacag
    37321 ggagctaagt cagttgaaaa agtcattcaa ggcttggaaa atgaactggt tattgtcagt
    37381 ggtctggcca agggcattga cacagcagct catatggcag ctcttcagaa tggcggaaaa
    37441 accattgcag tgattggaac aggactggat gtgttttatc ctaaagccaa taaacgcttg
    37501 caagactaca tcggcaatga ccatctggtt ctaagtgaat atggacctgg cgaacaacct
    37561 ctgaaatttc attttcctgc ccgtaatcgc atcattgctg gactttgtcg tggtgtgatt
    37621 gtagcagagg ctaagatgcg ttcaggtagt ctcattacgt gtgagcgagc aatggaagaa
    37681 ggacgcgatg tctttgctat tcctggtagc attttagatg gactatcaga cggttgccat
    37741 catttgattc aagaaggagc aaaattgctc accagtgggc aagatgttct tgcggaattt
    37801 gaattttaaa aatgacctaa gctagaattc taagaaaaaa tcaattttaa gagaaaatga
    37861 acccaacatt tccataataa aacgcatatt agcaagtttt taacacttgc taatatgcgt
    37921 tttttctaag tggattagta gagtagagga tttttctcat ataatactct tcgaaaatct
    37981 cttcaaacta cgtcagcttc catctgcaac ctcaaaacag tattttgagc tgagttcgtc
    38041 agtcttatct acaacctcaa agcagtgctt tgagcaacct gtggctagct tcctagtttg
    38101 cgctttgatt ttcgttgagt ataagggaaa gtatagtgaa ttgaaataag atgtgaacaa
    38161 ctctatcagg aaagtcaaat taatttatag aaatatttta gcagccaagg tgcactgtta
    38221 tagattcaat acactataat ttagtgtaat tgagaaagga gaaatgattg tgattgatgt
    38281 tggctaggtt atgttcaatg attcctacca tctcaaatct tgttagtaag gaaaaataaa
    38341 ttcttcaaaa gtagagatta caaggcttgt ttaagaaaga attcaaagac cttgacaaat
    38401 aaaaataaaa tggttattat aaaaaatggt ctgaaataga tgatgatact tttcgaaaat
    38461 ctttttacgt cagctcagct ttgccttgct gtgttttgag caagctacgg ttagcttccg
    38521 agtttgattt tcatttacta gaaatgaaac tgatgagaga tatcagtaga catttgagtc
    38581 aggatattat ggaaaatgat aaaaagagct cgtgagattg gcatatcaga ctactaaagt
    38641 attgagtttg ttaggatttt agcgactagt tagctgggaa aggaagatat ttgtgacaaa
    38701 taataaactg tattcgttga tagaatttag aaataaaata tatgaagaat tagaactttc
    38761 cagaagtgat ttagcgattt tactatgtgc catgcttatc gcctctatcg gattaaatat
    38821 ggattcgact cccgtgatta ttggagccat gttaatctct cctttgatga cacctattct
    38881 gggagtgggg ctctctctag ctatatttga ttttaaattg ttaagaaaat cttttaaaat
    38941 attagctatt caaattcttg ccagtttaat agcttcaaca ctttattttt atctttctcc
    39001 catttcgtat gctagttcgg aaattgttgc tagaacctct ccgactattt gggatgttct
    39061 cattgctttt gtaggaggga tagcaggtat tattggtgct aggaaaaaag agaccaataa
    39121 tattgttcct ggtgttgcta ttgcaaccgc cttgatgcct cctctttgta cagtaggtta
    39181 tgctattgct tctgctaatc taaaatttat cataggctcc tcttacctat tcctcatcaa
    39241 ttgtagcttt attgtcattg cgacttatat aggtgttagg ttgatgatgg ttaagaaaca
    39301 ttattttaaa gataatgaag aagactctaa aatgcgtagg attttgcttc tagttgctgt
    39361 tttgctgatg attccgagtt tcatctctgc aacgacttta gtgagagaaa cgttgaaaaa
    39421 agagtccctt aagaaattta tatcagagca gtttcagggg tataatattt tgaaaaaaac
    39481 ctattctaaa aagactcata ccctaaagct aaccatttca ggaaattatt tgacagaaga
    39541 agaactcgat atgatttcaa gtaagagagg tgactatggt ttaagtgatg tttctgttca
    39601 agtttcacaa ttgtctgatt cagaacaact tagtaaggaa gagctggtgg agcatttctt
    39661 ccagtatatc aaggataagg aagcaaaaga aaaggagaaa gccaataaat ttgatacaga
    39721 gtctgaggag caataatttc ttgagaatag ctggtttctc tcgtgagtct tctatgtata
    39781 tcaaaggaag actgaggttt taagtataaa acttttcttc tattatagta gctaagtctt
    39841 gacagggttc aaaaaatggt ttacacttta taaagtttat tactttgaaa aggtgtgata
    39901 ctgtggctac ggcaacaaaa aagaaaaaat caacagttaa aaaaaatcta gtcatcgtgg
    39961 agtcgcctgc taaggccaag acgattgaaa aatatctagg cagaaactac aaggttttag
    40021 ccagtgtcgg gcatatccgt gatttgaaga aatccagtat gtccgtcgat attgaaaata
    40081 attatgaacc gcaatatatt aatatccgag gaaaaggccc tcttatcaat gacttgaaaa
    40141 aagaagctaa aaaagctaat aaagtttttc tcgcgagtga cccggaccgt gaaggagaag
    40201 cgatttcttg gcatttggcc catattctca acttggatga aaatgatgcc aaccgtgtgg
    40261 tcttcaatga aatcaccaag gatgcagtca aaaatgcttt taaagaacct cgtaagatcg
    40321 atatggactt ggtcgatgcc caacaagctc gtcggatctt ggatcgcttg gtagggtatt
    40381 cgatttcgcc tattttgtgg aagaaggtca agaagggctt gtcagcaggt cgcgttcagt
    40441 ccattgccct taaactcatc attgaccgtg aaaatgaaat caatgccttc cagccagaag
    40501 aatactggac agttgatgct gtctttaaaa agggaaccaa acaatttcat gcttccttct
    40561 atggagtaga tggtaaaaag atgaaactga ccagcaatga cgaagtcaag gaagtcttgt
    40621 ctcgtctgac tagtaaagac ttttcagtag atcaggtgga taagaaagag cgcaagcgca
    40681 atgctccttt accctatacc acttcatcta tgcagatgga tgctgccaat aaaatcaatt
    40741 tccgtactcg aaaaaccatg atggttgccc aacagctcta tgaaggaatt aatatcggtt
    40801 ctggtgttca aggtttgatt acctatatgc gtaccgattc gactcgtatc agtcctgtag
    40861 cgcaaaatga ggcggcaagc ttcattacgg atcgttttgg tagtaagtat tctaagcacg
    40921 gtagcaaggt caaaaacgca tcaggtgctc aggatgccca tgaggctatt cgtccgtcaa
    40981 gtgtctttaa tacaccagaa agcatcgcta agtatctgga caaggatcag ctcaagctat
    41041 atacccttat ctggaatcgt tttgtggcta gccagatgac agcggccgtt tttgatacca
    41101 tggctgttaa attgtctcaa aaaggggttc aatttgctgc caatggtagt caggttaagt
    41161 ttgatggtta tcttgccatt tataatgatt ctgacaagaa taagatgtta ccggacatgg
    41221 ttgttggaga tgtggtcaaa caggtcaata gcaaaccaga gcaacatttc acccaaccgc
    41281 ctgcccgtta ttctgaagca acactgatta aaaccttaga ggaaaatggg gttggacgtc
    41341 catcaaccta cgcgccaacc attgaaacca ttcagaaacg ttattatgtt cgcctggcag
    41401 ccaaacgttt tgaaccgaca gagttgggag aaattgtcaa taagctcatc gttgaatatt
    41461 tcccagatat cgtaaacgtg accttcacag ctgaaatgga aggtaaactg gatgatgtcg
    41521 aagttggaaa agagcagtgg cgacgggtca ttgatgcctt ttacaaacca ttctctaaag
    41581 aagttgccaa ggctgaagaa gaaatggaaa aaatccagat taaggatgaa ccagctggat
    41641 ttgactgtga agtgtgtggc agtccaatgg tcattaaact tggtcgtttt ggtaaattct
    41701 acgcttgtag caatttccca gattgccgtc atacccaagc aatcgtgaaa gagattggtg
    41761 ttgagtgtcc aagctgtcat cagggacaaa ttattgagcg aaaaaccaag cgtaatcgcc
    41821 tattctatgg ttgcaatcgc tatccagaat gtgaatttac ctcttgggac aagcctgttg
    41881 gtcgtgactg tccaaaatgt ggcaacttcc tcatggagaa aaaagtccgt ggtggtggca
    41941 agcaggttgt ttgtagcaaa ggcgactacg aggaagaaaa gattaaataa gaggaggggc
    42001 ccgaaagtaa atttcaggct ccttttggtt agaacttgac aaaatacatc attttatgcg
    42061 aaactagaag aagattattt tatactcaat gagaatcaaa gagcaaatta gaaagctagt
    42121 cgcaggttgc tcaaagcact actttgaggt tgaagatgga agctgacctg gtttgaagag
    42181 attttcgaag agtattaact aggagaagtt atgcgtctta tctatctaat tattggtttt
    42241 ttatcgctgg ccttggctat tgttggggtt gttttaccct tgttgccgac aacgcctttc
    42301 cttttgttgt ctattgcttg tttctccaga agttccaagc gtttcgaaga ctggctttat
    42361 cataccaagc tctatcaaac atatgtagct gattttcgcg agactaagtc tattacgcgt
    42421 gaacgaaaga aaaaaatcat cgtctctatc tacgtcttga tgggaatttc tatttatttt
    42481 gcacctcttt taccagtcaa aatcggtctg ggtgctttga ccatctttat cacttattat
    42541 ctcttcaagg tcattccaga caaagaatag ttaaaacagt agttatttgc cttgataaaa
    42601 ttgaaagcat attcataaca atatgatata ataaatttga agtaatattc aaggagaatc
    42661 aaatgattta cgaattttgt gctgaaaatg tgactttact tgaaaaagcg atgcaggctg
    42721 gagctcgtcg gattgaactc tgtgataatc tagcagttgg tgggacaaca cccagctatg
    42781 gagtgactaa ggcagcggtt gaactggcag ctaactacga tacaaccatc atgaccatga
    42841 ttcggccacg tggtggtgac tttgtctata atgacctaga aattgctatc atgctagaag
    42901 acatttgttt gactgctcag gctggaagtc aaggggttgt atttggagct ttaactgctg
    42961 ataaaaagtt ggataagcct aatctggaaa agttaattgc tgcatcaaaa ggaatggaaa
    43021 ttgtctttca catggccttt gatgaactaa gtgatgaaga tcaaccggaa gctattgact
    43081 ggctcagtca agccggtgtc actcgtatcc taactcgtgc tggtgtgtct ggcgactcct
    43141 tagaaaaacg ttttgttcac tatcacagaa ttttggagta cgctaaaggt aaaattgaaa
    43201 ttctaccagg tggggggatt gaccttgaca accgtcaaac ctttattgac caggtggggg
    43261 taacacaatt gcatggcact aaggttgttt tttaaaaaat agaaaggaac tgctagcctt
    43321 gggtagcagt tttcacttat gtttgaaatt tttaaatcct atcaatttaa tcaagaaaag
    43381 gctcatgatt atggttttat agaaaatagc gaagtctgga catatagttg ccagattttg
    43441 caaggtgact ttgtcatgac tgtgtccatc actgctgata atgtgaactt tcaagtcttt
    43501 gaccaagaga ctggtgacct ctatcctcac gttcatatgg aaagcatgag gggaagtttt
    43561 gtcggaaatg tccgtgaggc ttgtctggag attctttacc agattcggaa ggcttgtttt
    43621 gatgtgcaag attttatctg tcatcagact aagcgtatca tgactcaagt tcaggaaaag
    43681 tatggaaacc agttggagta tctgtgggaa aaatcgcctg atacagctgt attgcgccat
    43741 gaaggcaatc aaaagtggta tgccgtcttg atgaaaatct cttggaataa gctggaaaag
    43801 ggcagagaag gacaagtgga agcagtcaac ctcaagcatg accaagtagc taatttgctt
    43861 tcacaaaagg ggatttatcc agccttccat atgagcaagc gctactggat tagtgtgtcc
    43921 cttgatgata ctttatcaga tgaagaagta ctggaattga tagaaaaaag ttggaactta
    43981 acctctaaaa aatgaaatat tttaataatt ttcatgaact ttcaattagc taaatattct
    44041 ttactgaaga aattttcaga aaatatagga tttaccacac cagaggaatg tggtgccatc
    44101 ttcaaatacc tgattgagaa tgtcaagacg gatcgtcaga tcatctattc gcctcactgt
    44161 catgatgacc tcggaatggc agtggcaaat agccttgctg ctgtcaagaa tggtgcagga
    44221 cgtgttgaag ggactatcaa tggtattagg gagcgagctg aaaatgctgc tttggaagaa
    44281 attgcagtgg ctctcaatat tcgccaagat tactaccaag tagaaaccag tattgtccta
    44341 aatgagacca tcaatacgtc agaaatggtt tctcgcttct ctggtattcc agttcctaaa
    44401 aacaaagccg tcgttggtgg caataccttc tcccacgaat ctggtattca ccaagatgga
    44461 gtccttaaaa atcctctcac ttatgagatc atcacacctg aattggttgg tgttaagatt
    44521 ccgcttggaa aattatctgg tcgccatgct tttgttgaga aactgagaga attggcccta
    44581 gattttacag aagaggatat caaaccactc tttgctaagt tcaaggcact ggccgataag
    44641 aagtaagaaa tcacagatgc agatactcga gctttggtag ctggaaccat ggttgaaaat
    44701 ccagaaggct tccactttga tgatttacaa cttcaaactc atgcagataa tgacattgaa
    44761 gcgctcgtta gcctagccaa tatggatggt gagaaagtcg aatttaatgc gacagggcaa
    44821 ggttccgttg aagcaatctt taacgctatc gataagttct ttaactaatc tgttcgtttg
    44881 gtgtcctaca ctatcaatgc ggtaacagat ggaatcgatg cccaggatcg ggttttggtc
    44941 actgttgaaa acagagatac agaaaccatc tttaatgcag cagggcttga ttttgatgtg
    45001 ttgaaggctt ctgctattgc ctatataaac gctaatacct ttgttcaaaa agagaatgca
    45061 ggtgagatgg gacgcagtgt ttcttaccac gatatgccta gtgtgtaaag gagaaggcta
    45121 tggcaaagaa aatagtagct ctagtaggag acggaattgg cccagaaatc atggaggctg
    45181 gtttagaagt tctggaggct ctagctgaaa aaacaggttt tgactatgag attgacagac
    45241 gaccgttcgg aggtgcagat attgatgcag caggacctcc cttacctgat gaaaccctta
    45301 aggcaagtag ggaagcagat gctatcctac tagcagctat cggtagtcct cagtatgatg
    45361 gagcagcggt tcgccctgaa caaggcctga tggctctccg taaggaactc aatctttacg
    45421 ctaatattcg tcctgtaaaa atctttgaca gtctcaagta tttgtcacca ctcaaaccgg
    45481 aacgaatttc tggtgtagac tttgtcgtgg tgcgtgaatt gacaggcgag atttactttg
    45541 gagatcatat tcttgaagag cgcaaagcgc gtgatatcaa cgactatagc tatgaggaag
    45601 tggagtggat tattcgcaaa gcctttgaaa ttgcaagaaa tcgcagaaaa atccttacta
    45661 gtatcgataa gcaaaatgtt ctagcgacct caaaactctg gcggaaagta gctgaggaag
    45721 tcgcacagga tttctcagat gtaaccttgg aacaccagct ggtagactca gctgctatgc
    45781 ttatgattac caatcctgct aagtttgatg ttattgtaac ggagaatctt tttggagata
    45841 ttttatctga tgaatcaagc gtcttatctg gtacacttgg ggttatgcca tcagccagtc
    45901 attctgaaaa tggaccaagt ctctatgaac ctatttacgg ttcagcacct gatattgcag
    45961 gtcaaggaat tgccaatcct atttccatga ttttatcagt tgccatgatg ttgagagata
    46021 gtttcggagg ttatgaggat gcagagcgta tcaaacgtgc tgttgagaca agtctggcgg
    46081 caggaatttt aacgagagat ataggaggtc aggcttcaac aaaggaaatg acggaagcta
    46141 ttattgcaag gttatgaagt tagacgaaaa aattactcta gtccttttga tttggaatgt
    46201 catcattttc ttgatttatg gcattgacaa attcaaggca agaagaagag cttggcgcat
    46261 ccaagagaaa atcttactca ttttagccct tacttgtggt ggttttgggg cctggttagc
    46321 tggaatcact tttcaccaca agactcgaaa atggtacttt aaaacagttt ggtttcttgg
    46381 gatggtgacc acactagtag ccttatattt tatttggagg taatggatgg cagggtcttc
    46441 gagggaatac gctgcttggg ctctagcgga ctatggtttt aaggtcgtga ttgcaggatc
    46501 tttcggtgac attcattaca ataatgaact caataatggc atgttgccaa tcgttcagcc
    46561 tagagaggtt agagaaaagc tagcccaact caaaccgacc gaccaggtaa ctgtagactt
    46621 ggaacaacag aaaattatct caccagttga agaattcacc ttcgagatag atagcgagtg
    46681 gaaacataaa ctcctaaata gtttggatga tatcggtatt accttgcagt atgaagagtt
    46741 gattgctgct tatgaaaaac aacgaccagc ctactggcag gattagaaaa aatagaaaag
    46801 gaaatatagt aaactgaaat aagatgtaaa caaatgaatt ggagcttaac atccatttcc
    46861 agcaattttt tagaaactac agtggactat tctggattca acacattata aaattatgac
    46921 aaaacacatt cacaagaagg ctacgacatt ttaaaaggtg agggcggatg tatcgtttgc
    46981 cctactaaag ttggttacat tatcatgacc agcgataagg caggacttga gcgtaagttt
    47041 gcagccaaag aacgtaagcg taacaaacca ggtgttgttc tctgcggtag catggatgaa
    47101 ctttgcgctt tagcgcaact caacccagaa attgaagcat tctactaaaa acattgggat
    47161 gaagatattc ttcttggttg tatccttcct tggaaaccag aagcctttga aaaattcaaa
    47221 gcatacgggg atggccgtga agaacttatg actgatgtac gtggtactag ctgttttgtt
    47281 atcaagtttg gaaaagcaga tgaacaattg gctgccaagc tttgggaaga aggtaaaatg
    47341 gtctacgcct catctacttc aatgataaaa cgattgaaac tcgctatgag agcaaggtgt
    47401 aatggtgtct atggtcgata agaacggcaa actcatccca gaacaaggag gagcacgttc
    47461 aacttcacca gctccagttg tgatccgtaa agggcttgac attgataaaa tcatgatgca
    47521 cctgtcagat acttttaact catgggacta ccgtcaggtt gagtattatt aggatagaga
    47581 agaagtctag tgttaaggac attacagctc ataatactag gcttttttat agaaattctt
    47641 ttctttttct ataggatatg gtattctatg tagaaaatat atgttaataa gtaatgccaa
    47701 tatttaaaca tcattagtaa aaggagttag attgatgaat aaaagaaaag ttagtttaga
    47761 agatttttat aaatggtata gtctaaataa agaaaagtta ttaaataagg caactgttgg
    47821 tgaaaagttt aatgataaat taaaagaaga gtttctccag gaatggcctt tggataggat
    47881 tttaacaatg tcaatcgatg aatatgtaat aggaaaggga cagcaaaata agtctttatg
    47941 ctacgctctt gagaagggaa aatacaaaaa tctatttctt ggaatttctg gtggctcagc
    48001 ttcaaaattt ggtatttatt ggaataaaaa aacaaacaaa tataaagatc aagctaataa
    48061 tgagatttca gagttggatc agcgattttc aaaattaaaa tcagatttgt atgaaattat
    48121 caaagaaggt attcgtttta actttgaaaa tcctattttt gatatgaaaa gatcaacaaa
    48181 tgaatttatt ggtcgttctg ctatggtgac aaaattactt tgtatctatt ctgggggaga
    48241 tcctttcttt ggtgtaaata ttaatagtca gaaagaattt tggaaccact ttgtttctca
    48301 gacaaatcaa ggtggacctt atctgcaaaa tcataaaata attgaactgg tgtccaaaac
    48361 ttatcctgag ttggagccat cgaaattagg aactatgctt tttgagtatt ctaagctttt
    48421 tatggaaaat aaggaagaca atagtacaat ggattcatca aacaattttc gtcatcaatt
    48481 aactcaatct ctattaaagt ctccaaacct tatccttcgt ggtgctcctg gcacaggaaa
    48541 gacttatctt gctaaagaaa ttgccaaaga attaacggat ggcaacgaag accaaatcgg
    48601 atttgtacaa tttcacccat cctatgatta tacggatttt gtagaaggtt taagaccagt
    48661 atcaaatggg gatggagcta ttgagtttag gctacaggac ggtattttta aagatttttg
    48721 tcagaaagca aaagaaaccc aattgattgg aggacaagat aattttgatg aggcttggga
    48781 ttcttactta gaatatataa atgttgctga agaaaaagaa tatataacaa aaacatctta
    48841 cttatctgtt aatagtagac aaaatttgtc agtaaattat gatagtggtg ttccaggatg
    48901 gtcactacct agcaaatatg tttacgagtt gtataaagat aaaaattata ataagcaaga
    48961 atactacaaa agtggtggaa aaactgtcct agaaacattg agaaagagat ttggtttgaa
    49021 agactatgtt tccccaacag aaattgatac tgataagaaa ttcgtcttca tcatcgatga
    49081 aatcaatcgt ggggagattt ctaagatttt tggcgaactc tttttctcta tcgaccctgg
    49141 ctatcgtggt gaaaaaggaa gtgtttctac ccaatatgca aatctacacg aaactgatga
    49201 aaagttctat atccccgaaa atgtttacat catcggaact atgaatgata ttgatcgttc
    49261 agtggatacc tttgattttg ctatgcgtcg tcgttttcgt tttgttgaag ttactgccga
    49321 gggtcaagtt ggcatgttgg ataaagagct gaatatccat gcagaagaag caaaaattcg
    49381 tctaagaaac ttgaacgctg ctatcgaaaa tgttcaggaa ttaaacagtc attatcatat
    49441 tggaccaagt tattttctta agttgaagga tgtagatttt gactatgaat tactctggtc
    49501 tgattatatt aagcctctcc tagaagacta cttgcgaggt tcttatgatg aggttgaaac
    49561 tttggaaact ttgaaaaaag catttgaact gacaaataat gagcaaaaag atcaggcagt
    49621 agctgatgac aatgaaggtg atgaaaacga tgatgcggat tactgataat caacacaaga
    49681 ttattaaaga aaaatttgtt gaagaatatc ctaaactaag caatcttctt ttagacagaa
    49741 ccttggaaag tctatcccaa gatgaacgta ttttcatttt tccaaatgat ttgactcata
    49801 ctcctgattt ggataaggac caaaagattt ttgaaacagt caatcagaaa atcaagacag
    49861 ggaacgtgat tggttttctt ggatatggtc aggaaagatt aacgatttcc tcacgatttt
    49921 ctgatgagag taatgaccat tttttgcatt atctcttaaa caaggttctt catatcaatc
    49981 tcactagttt agatgttgct ttgtctcgtg aagagaggct ttatcaactt ttggtgtatc
    50041 tctttcccaa gtatctacaa gctgctattc gaaaaggtct ttataaggaa tatcatcgat
    50101 tttctcataa cgacagtaat gttaagggag tgattgatgt aagaagccat ctcaagaaaa
    50161 atcttccttt cacgggaaat attgcctacg caacgagaga gttcacctat gataatcccc
    50221 tcatgcagtt ggtccgtcac actattgaat acattaagaa tcagaaaagc attggtcaag
    50281 gggtactaga taatctctca actagtcgtg aaaacgtatc tgaaatcgtg cgtgtaacgc
    50341 cctcttataa actagctgat cgtgctaaga ttattcgggg aaatcaatct aaacctatac
    50401 gtcatgcata ctttcacgag tacagaaact tacaagaact ttgtctgatg atcctaaacc
    50461 aagaaaagca cggtttaggg tatcaagatc aaaaaatcta tggtattctc tttgatgttg
    50521 cctggctttg ggaagagtat gtttacacct tgttgccaaa aggttttgta catcccagaa
    50581 ataaggataa gacggatgga atttcagtat tttctgttgg gaaacgaaaa gtatatccag
    50641 atttttatga cagagaacga aagattgttc tagatgcaaa atataaaaaa ctggaattga
    50701 ctgaaaaagg aatcaaccgt gaggacttat tccagctgat ttcctattct tatattttaa
    50761 aagctgagaa ggctggactg atttttccta gtatggagca gtcagtaaat agtgaaatag
    50821 gaaaagtagc tggctatgga gctcaattga agaagtggtc tattcgaatc cctcagaatg
    50881 cctcattcta tagtacattt tgtaaaatga tggaaaattc agaagaaaat tttaaagcga
    50941 ttattgatga agaagtgggg agaaaataat ttcccctctt tcttttattt ttaacacaag
    51001 taaaacccga acgatatatt ttaattcata aaataatttg acaaaaactg tactttggtt
    51061 tataataaat taaggaaacg tcggaaaagg cgtagtgatg caagagcata ggtaggtcat
    51121 tacaaaagaa acgagacatc gatatgttaa atgaatttcc aatttttgat tacgaagata
    51181 ttcaattgat tccaaataaa tgtgtcatta aaagccgtgc agaagcggat acaagtgtca
    51241 ctttaggaaa tcacaccttt aaactacctg ttgtgccagc gaatatgcag acgattttgg
    51301 atgaaaatgt agcagagcaa ctggctaaag gtggttactt ctacattatg caccgttttg
    51361 atgaggcagg acgcattcct tttattaaac gaatgcacga tcaagggctc attgcttcta
    51421 tctctgtcgg tgttaaggat tatgagtatg atttcgttag acagctcaaa gctgatgctc
    51481 ctgagtatat cactattgat attgctcatg gtcatgcgga tagcgtgatt tctatgattc
    51541 aacacatcaa gaaagaattg ccagatacct ttgtcattgc tggaaatgtg ggaacaccag
    51601 aagctgtgcg tgaattggaa aatgctggtg cggatgctac taaggtcgga atcggtcctg
    51661 gtaaggtttg tatcaccaag gttaagactg gttttggtac aggtggttgg cagttggctg
    51721 ctctacgctg gtgtgccaag gctgcgcgta aaccgattat cgctgatgga ggaattcgta
    51781 ctcacggtga tattgctaag tctatccgct tcggtgctag catgatcatg attggttccc
    51841 tctttgcagg acacattgaa agtccaggga aaacgattga agtcgatggt gaacagttca
    51901 aagaatatta tggttcagcc tcacaatatc aaaaaggtgc ttacaaaaat gtggaaggca
    51961 aacgtatctt gcttcctgct aaaggtcatc tgcaagacac tttaactgag atggaacaag
    52021 accttcaaag tgctatttct tatgcaggtg gacgtcaggt tgctgacctt aaacacgttg
    52081 attatgtgat cgtgaaaaac tccatctgga atggggatgc ttcccactaa gacggggtct
    52141 ttgaccaaaa aacttgttat tagagcaaat ttctgttata ataaaacaag tttccaccct
    52201 tagtgtaatg gatatcacgt aagattccgg ttcttgagat gggggttcga ttccctcagg
    52261 gtggatgtaa acatcctaaa aagcctttaa tagggctttt ttctttatcc tgacccaaat
    52321 ttgccgctaa aactgagaat ctctgtctaa agaattccca aatgctttcc agtcccgttt
    52381 tagagtgact cagggggctt tttttgatat aataaaaagg actattatca gttagaaaga
    52441 ggttggtatg aaagaattac aaactgtact aaagaatcat tttgcaatcg aatttgcaga
    52501 caaaaattta ctggaaactg cctttactca tacgagttat gccaatgagc accgcctctt
    52561 aaaaatttca cacaatgaac gcttggaatt tttaggagac gctgttctac agttattgat
    52621 ttcagaatat ctatataaaa aatatcctaa aaagcctgaa ggtgacctat caaaactccg
    52681 tgctatgatt gtccgtgagg agagtttagc tggttttgcg cgtgattgcc agtttaacca
    52741 gtttatcaag ttgggtaaag gggaagaaaa atcaggtggt cgcaatcgtg acaccattct
    52801 tggtgatgcc tttgaagcct ttcttggtgc ccttcttttg gataaggatg tggccaaggt
    52861 caaggaattt atctatcaag tcatcattcc taaggttgaa gcaggcgagt ttgagatgat
    52921 tacagactat aaaacccatc tccaagagtt gcttcaggtc aatggtgatg tggctattcg
    52981 ttatcaggtg atttctgaaa cagggcctgc tcatgataag gtctttgatg tagaagttct
    53041 tgttgaaggt aagagcatcg gtcaaggcca aggtcgttct aagaaattag cagagcagga
    53101 agctgccaaa aatgccgttg agaaggggct ggattcatgt atttaaagga aatcgaaatt
    53161 caggggttca agtcttttgc tgataagacc aaggtcgttt ttgaccaagg tgtgacggca
    53221 gttgttggac ccaatggatc tggaaagtcc aatattacag aaagtctgcg ttgggctttg
    53281 ggggagtcta gtgtcaagag tctccgtggg ggcaagatgc cggatgtcat ctttgctgga
    53341 accgaaagtc gcaaaccgct caattatgct tctgtagttg tgactctgga taatcatgac
    53401 ggatttatca aggatgcagg tcaagaaatc agggtggaac gccatatcta tcgtagtgga
    53461 gatagcgaat acaagattga cggcaagaaa gtccgtctgc gtgatattca tgacctcttc
    53521 ttggatactg gattgggacg agattccttc tctattattt cccaagggaa ggttgaggag
    53581 atttttaatt ccaagcctga ggaacgacga gctatttttg aagaagctgc tggagtttta
    53641 aaatacaaga ctcgcagaaa agaaaccgag agtaaactgc agcaaactca ggataatctg
    53701 gaccgcttag aggacattat ctacgagttg gataatcaaa tcaagcctct tgagaagcaa
    53761 gctgagaatg cccgtaagtt tttagacttg gaaggacaac gtaaggctat ttatttagac
    53821 gttctggttg ctcaaatcaa ggaaaataag gctgaactag agtcgacaga agaagagttg
    53881 actcaggttc aagaactctt gatgagttat taccaaaagc gtgaaaaatt agaagaagaa
    53941 aatcaaactc ttaaaaagca acgccaagat ttacaggctg aaatggccaa agaccaaggc
    54001 agtttgatgg acttgactag tctgattagt gatttagaaa gaaaattagc cctatcgaaa
    54061 ctggagtccg agcaagtggc cttgaatcaa caggaggcac aagcccgttt ggctgctttg
    54121 gaggataaga gaaattcact cagcaaagaa aagtctgata aagaaagctc tttagctctg
    54181 ttagagggaa atctagtcca aaataatcaa aaactcaatc gtttagaagc tgaattgctg
    54241 gctttttcag atgatcctga tcagatgatt gagcttttgc gtgaacgctt tgtggcgctt
    54301 ttacaagaag aagcggatgt ctcaaaccaa ctgacccgca tcgagaatga gttggaaaat
    54361 agtcgtcagc tttctcaaaa acaagcagat caactagaaa agctgaaaga gcaattggct
    54421 atagctaaag agaaggctag tcagcaaaag gaagagcttg aaactgccaa ggagcaggtt
    54481 cagaaattat tggctgacta tcaagctatt gccaaggagc aagaggagca aaaaacttcc
    54541 tatcaagctc aacaaagtca actctttgac cgtctggata gtcttaaaaa caagcaggct
    54601 agagctcaaa gtttggaaaa catccttaga aatcacagta acttttatgc aggtgtaaag
    54661 agtgttctcc aagaaaaaga ccgccttggt gggattattg gtgctgttag tgagcatttg
    54721 acctttgatg tgcattatca aactgcctta gagattgcac ttggagccag cagtcagcat
    54781 atcatcgtag aagatgaaga gtcggcaacc aaagctattg atttccttaa acgaaacaga
    54841 gccggtcgtg caaccttcct tccgttgaca acaatcaagg ctcgtacgat ttctagccag
    54901 aatcaagatg ttatcgctgt aagcccaggt ttccttggga tggcagatga gttggtgact
    54961 tttgatacta gactggaagc cattttcaag aatttgctag ctacgacggc tatttttgat
    55021 actgtagagc atgcgcgtgc agcagctcgc caagttcgtt atcaggttcg tatggtgaca
    55081 ttggatggga cagaattacg cacgggtggt tcctatgcgg gtggtgccaa tcgccaaaat
    55141 aacagtattt tcatcaagcc agaactggag caattacaaa aagaaattgc tgcagatgaa
    55201 gcaagcttgg gttcagaaga agcggctttg aagaccttgc aagaccagat ggctacattg
    55261 acagaaagat tagaagccat caaatctcaa ggagaacagg cacgtattca ggagcaaggc
    55321 ttgtccctcg cttatcagca aactagtcag caagttgaag aactggaaac tctttggaaa
    55381 ctccaagaag aggaaaaaga tcgtctttct gatggagatt ggcaagcaga caaggaaaac
    55441 tgccaagagc gtcttgctgc aatcgccagt gacaagcaaa atctggaagc tgagattgaa
    55501 gagattaagt ctaataaaaa tgccatccaa gaacgctatc aaaacttgca ggaagagcta
    55561 gcgcaagctc gtttgcttaa gacagaactg caagggcaaa aacgttatga agtggctgat
    55621 attgaacgct taggcaagga attggacaat cttgattttg aacaagagga aatccagcgc
    55681 cttcttcaag aaaaggttga caatcttgag aaggttgata cagaattgct cagtcaacag
    55741 gcggaagaat ccaaaactca gaaaacgaac ttccaacaag gtttgattcg caaacagttt
    55801 gagttggatg atatagaagg tcagctggat gatattgcta gtcatttgga tcaggctcgc
    55861 cagcagaatg aggagtggat tcgcaagcaa acacgtgctg aagccaagaa agaaaaggtc
    55921 agcgagcgct tgcgccatct acaaagtcaa ttaacagacc agtaccagat tagctatact
    55981 gaagctctag aaaaagcgca tgaattggaa aacctcaatc tggcagagca agaagttaag
    56041 gatttagaga aggctattcg ctcactgggt cctgtcaata tagaagctat tgaccagtac
    56101 gaagaagttc gcaaccgtct ggactttcta aatagtcagc gagatgatat tttgtcagcg
    56161 aaaaatctgc tccttgaaac cattacagag atgaatgatg aggtcaagga acgctttaaa
    56221 tcaacctttg aagctattcg tgagtccttt aaagtgacct tcaagcagat gtttggcgga
    56281 ggtcaggcag acttgatatt gactgagggc gaccttttaa cagctggtgt ggagatttct
    56341 gttcaacctc caggtaagaa aatccagtcg cttaacctca tgagtggtgg tgaaaaagct
    56401 ctatcggctc ttgccttgct tttctccatt attcgtgtca agactattcc atttgttatc
    56461 ttggatgagg tggaagctgc gctggatgaa gctaatgtta aacgttttgg ggattacctc
    56521 aaccgctttg acaaggacag ccagttcatc gtcgtaaccc accgtaaggg aaccatggca
    56581 gcggctgatt ctatctatgg agtgaccatg caagaatcag gtgtctcaaa aattgtttca
    56641 gttaagttaa aagatttaga aagtattgaa ggatgacaat taaactagta gcaacagata
    56701 tggatggaac cttcctagat gagaatgggc gctttgatat ggatcgcctc aagtctctct
    56761 tggcttccta taaggaaaaa gggatttact ttgcggtggc ttcgggtcgg ggatttctgt
    56821 ctctggaaaa attatttgct gatgttcgtg atgacattat tttcatcgcg gaaaatggca
    56881 gtttggtaga gtatcaaggt caggacttgt atgaagcgac tatgtctcgt gacttttatc
    56941 tggcaacttt tgaaaagctg aaaacgtcac cttatgtaga tatcaataaa ctgctcttga
    57001 cgggtaagaa ggggtcatat gttctagata cggttgatga gacctatttg aaagtgagtc
    57061 agcattataa tgaaaatatc caaaaagtag cgagtttgga agatatcaca gatgacattt
    57121 tcaaatttac aaccaacttc acagaagaaa cgttagaagc tggtgaagct tgggtcaatg
    57181 ataatgtccc tggtgtcaag gctatgacaa ctggctttga atctattgat attgttctgg
    57241 actatgtcga taagggtgta gctattgttg aattagctaa aaaacttggt atcacaatgg
    57301 atcaggtcat ggcttttgga gacaatctta atgacttaca tatgatgcag gttgtgggac
    57361 atcctgtagc tcctgaaaat gcacgacctg aaattttaga attagcaaag actgtgattg
    57421 gtcaccataa ggaacggtcg gttatagctt atatggaggg cttataatgg cagatataaa
    57481 attgattgca ttggacttgg acgggacctt gctgactact gataaaaggc tgacggatcg
    57541 taccaagtca accttgaaag ctgcgcgtga tcgtggtatc aaggtcgtat tgacaactgg
    57601 tcgtccctta aaagccatgg acttctttct ccatgagtta gggactgacg gccatgaaga
    57661 tgagtatacc attactttta atggtggttt ggttcagaaa aatacaggtg aaatccttga
    57721 taaaacagtc ttttcatatg ctgatgtggc acgcttgtat gaggaaacag agaaattatc
    57781 actgcctctt gatgccatct cagaaggaac agtttatcaa atccaatcgg accaagaaag
    57841 tctttatgcc aaattcaatc cagctttgac ttttgttcca gttgactttg aagacctgtc
    57901 tagtcaaatg acctacaaca aatgcgtgac tgcctttgct caagaaccct tggatgcagc
    57961 cattcagaag atttctccag aattgtttga ccaatatgaa atctttaaat cacgtgaaat
    58021 gttgctagaa tggtcaccaa agaatgttca taaagcaaca ggtttggcaa aactaatcag
    58081 ccatcttgga atcgaccaaa gccaagtgat ggcttgtggt gacgaggcca atgacctctc
    58141 tatgattgaa tgggcaggtc ttggtgttgc tatgcaaaac gctgttcctg aagtaaaggc
    58201 agccgcaaat gtagtgacgc cgatgaccaa cgatgaggaa gctgtcgcct gggctatcga
    58261 agaatatgtg ttaaaggaga actaagatat gggattattt gaccgtctat tcggaaaaaa
    58321 agaagaacct aaaatcgaag aagttgtaaa agaagctctg gaaaatcttg atttgtctga
    58381 agatgttgat cctaccttca cagaagttga ggaagtttct caggaagaag cagagatgga
    58441 aattgttgaa caagctgtgt tccaagaaga ggaaatccaa gacacagttg aagaaagtct
    58501 ggatttagag ccagttgtag aagtttctca aaaagaagta gaagaatttc caaactcaca
    58561 agaagtcaca gaggaagaga agcttgagca cgaaggaact gtagaagaaa ataattctga
    58621 agtgcttgaa ccagaagctc ctcaaacaga agaaactgtt caggaaaaat atgaccgcag
    58681 tcttaagaaa actcgcacag gtttcggtgc ccgcttgaat gccttctttg ctaacttccg
    58741 ctctgttgac gaagaatttt tcgaggaact ggaagaactg ctgattatga gtgatgttgg
    58801 tgtccaagtc gcttctaact taacggagga actacgttac gaagccaagc ttgaaaatgc
    58861 caagaaacct gatgtacttc gtcgtgtcat cattgagaaa ttggttgagc tttatgaaaa
    58921 ggatggtagc tacgatgaaa gcatccactt ccaagataac ttgacagtta tgctctttgt
    58981 tggtgtgaat ggtgttggga aaacaacttc tatcggaaaa ctagcccacc gctacaaacg
    59041 agctggtaag aaggtcatgc tggttgcagc agataccttc cgtgcgggtg cagtagctca
    59101 gctagctgaa tggggccgac gagtagatgt tccagtagta actggacctg aaaaagctga
    59161 tccagccagc gtggtctttg atggtatgga acgtgccgtg gctgaaggta tcgatattct
    59221 catgattgat actgctggtc gtctgcaaaa taaggataac cttatggctg agttggaaaa
    59281 gattggtcgt attatcaaac gtgttgtgcc agaagcacca catgaaacct tcttggcact
    59341 tgatgcatca acaggtcaaa atgccctagt acaggccaaa gaattttcga aaatcacacc
    59401 tttaaccgga attgttttga ctaagattga tggaactgct cgaggaggtg tggttctagc
    59461 cattcgtgaa gaactcaata ttcctgtaaa attgattggt tttggtgaaa aaatcgatga
    59521 tattggagag tttaactcag aaaactttat gaaaggtctc ttggaaggtt taatctaatc
    59581 agaagcaaaa atcctgcaag gcataaactt gcaggaaatt tttttattct aagcgaccat
    59641 cttgacgata ggcgatatct ggttgccaag tccatttggc accgaatttt tcaagtaggt
    59701 caaagctggc ttgaggtccc atgcttccag ctttatagtc atgaagtggg gcaccatttt
    59761 cagcccagag cttttcaata cgatcaatca acttccatga cgcaccaact tcatcccagt
    59821 ggctaaagtt agttgagttg ttatttagga catcataaat caatttttcg tatggttctg
    59881 gagaagcacc agttgcagtc gcgtctgtac gataatcaag tgagttagga gccaagttaa
    59941 attcttctcc tacttgcttc ccatttaggc taagagagaa gccttctgtt ggttgaatat
    60001 agatggtcaa aatatttgga gcaagtggtt ctccaaagat agaatccatt tgtttaaaga
    60061 cgatgttgac atgagttcct ttttcagtca gtcgtttacc tgtacggaaa aagaaaggaa
    60121 caccacggaa tcgatcgctg tctacaaaga aggcaccaga tgtaaaggtt tcagttgttg
    60181 attctggatt cacatttggc tcgctacgat aagagatgta ttttatgcca tcaatcttac
    60241 cagagcggta ttgcccacgg ataaagtgtt ctttgagttc ttcatcagtt ggatgataga
    60301 ggtttttaaa gaccttaatc ttttcagcac gaatctcgtc ttttgtgaag cttgctggtt
    60361 tgtccatggc gagaagcgaa agaagttgta gagtgtggtt ttggaccatg tcacggaggg
    60421 caccggattg gtcatagtag ccaccacgtt cttctacacc caagcgctcc gcaaaggtaa
    60481 tttgaacatt gtcgataaaa tccttgttcc aaacgttttc aaaaatcaag tttgcaaagc
    60541 gaactgcaaa gatgctttgg atcatttcct taccaagata atggtcgata cggaaaattt
    60601 gttcttcgtc aaatgttgct aggagttcgt cattcaactt gcttgcagtt gcgtaatctg
    60661 taccaaatgg tttttcaacg atcaagcgct caaaaccttt gccatcgaca atgttttcag
    60721 atttgaggtg tttggcaatg gttccaaaga actgaggtgc catagacaag aagaagagct
    60781 tattgtgttc agcttggtat ttttcattaa gctcagcttg taattgacgc aaagcaatat
    60841 aatgttccga atcattgaca tcatggcttt gatagtagaa gtggctagca aattcttggg
    60901 cttgctcggt actatctgcc aaatcaagga tggactcgac aactacagat tcaaaatatt
    60961 ccttactcca aggtctacgg gcagttccaa taacggcaaa gtgcttggaa agattgccgg
    61021 atttatatag tctaaaaagg gaagggtaga gcttgcgttt agccaggtct ccactcgcac
    61081 cgaaaattgt aacaataacc ttagatgaca tctagctacc taatttctat ttttattcct
    61141 agtcttgcta ggtatgagga aacctcattt cataaactta attctaacat aattttccga
    61201 aaaatcaaaa attccaatac gaggtagaaa aactgcaagg aaatccttac agtttgagtt
    61261 tagacgttta agaccttatc taagaactct ttcagacgag ggtgttgtgg gttatcaaag
    61321 atttggtcag gtgttccgtc ttcaaggaac tcgccatctg cagtaaagat aacgcggttg
    61381 gcaacctgac gagcaaatcc catctcatgg gttacgataa tcatggtcat gccttgctca
    61441 gccaattcct tcataacgtt aagtacgtct ccaaccatct cagggtcaag ggcagaagtt
    61501 ggttcatcga agagcatgat gtctggattc attgctaggc cacgagctat ggccacacgt
    61561 tgtttttgac cacctgatag gctatctgga ttggcattag ctttatctgc tagtccaacc
    61621 ttttcaagca actccattcc caatttctca gcttcttcct tagtcatcaa cttgtgctca
    61681 ataggagcaa aggtgatgtt gtccaataca gacatatgag ggaagaggtt gaagtgttgg
    61741 aataccatgc cgatattttc acggacgtgg tcaacattgg ttgttttttc agttaaatca
    61801 tagccgttca cagtgatgtg accgctagtg acttcttcta aaagattgag gctacggagg
    61861 aaagttgact taccagaacc tgaaggaccg atgatacaaa caacatctcc ttcatagaac
    61921 ttagtcgtaa ttccttttag gacttcattt tttccatagt gcttgtgtaa atcatttaca
    61981 tcaattttta attttgccat taacgaatcc tcttttctaa gcgtttcgct agtctagtca
    62041 aaagtgtgat aattacaaga tagaagatag caaggattgc atacatcttg aaactttggt
    62101 agttacgagc aatgataatc ttaccagttt ggaagagttc aaccaaaccg atagcagata
    62161 cgatagttgt atctttaaga gcgataacga attggttgac aaagtttggc aacatcaatt
    62221 tagttgcttg tggcaagata atcttacgca tggtttttcc ataagagata cccaagcttc
    62281 ggctggcttc catttggcca actggaacgg cctgaatacc accacgaacg atttcagcga
    62341 tataagcagc cgcattgagt gagagggcaa ttgttccagc tacaaagtcg ttaattgggc
    62401 tttgttggcc tgtgatagac tcgatgaagt ttggaattcc ccagaagatg aaggctgcaa
    62461 gaatcatcaa tggaatacca cgaataacgt caacgaaaat ctcagagatg acgcgaagag
    62521 atttgtatgg gctaacgcta aacataccga agataattcc gatgacaatg gcaatagcaa
    62581 atgagataag agctagagca agagtgatac caagaccgct aaggagttgt ttgtagttgt
    62641 tttgaagcaa gccccagagc gttgtttcgt caacagtact tgttgaagca gttgaagatt
    62701 cgctagctag gtatttgtca agaatctttt ggaattcacc gtttgcttta aggtttgcaa
    62761 gtccgttgtt gaacatttca atcagttctg gatttgctcc ttttttaacg gcaaaggctg
    62821 tttcaccgat tggagttcca gagattggag ttttcaattt ttgaccttgg ctgatagaat
    62881 atttgagaac aggttcatca tccataacgg catcaatggc accagtgttt aaactgtcat
    62941 acattgaaga accatcagca aaggttttga ttttgtagcc gtatttgctt tgattttctg
    63001 ttaggaaggt ttgagaagca gttccgtttt taacaccgac tgtctttcct tttagatctt
    63061 cataagaagc aatattactt gattctttga caccaagaat ggtattagca gtgtagtatg
    63121 attctgagaa gtcaaaagtt gccttacgag catctgtgac agacatacca gcgatgatac
    63181 catcggcttg accagcttgg acagcactga tagcagcatc aaaaccaggg ttggtgattt
    63241 caatttcaaa accttggtct ttagcgattg ccttaatcaa ttccatatca ataccagtgt
    63301 attggttgct tgaattttgg aaaacaaaag gggcaaaaga agaatcgctg gcaatgatat
    63361 atttagcctt aacaggaata gcttttaatc ctgcgagagt agttgtagtt ggcactgctg
    63421 aagatgatga agcagtccat ttcttgataa ttttatcaag actaccatct tttttcattt
    63481 cagacaaggc ttggttaaat tcagtaacca ggtgctcgta tttacttcct tttttcacac
    63541 cgaaagcaaa acttcctaca gcttcaccat ccatttcaat atggaggtct tgaccttggt
    63601 taatggcata ttcgataaca ggtttgtcat ccatcatggc atcgatggca ccagcactca
    63661 agctgttgtt cattaaatca ccagtgtcaa atgttttaat agtaaagcca tatttatctt
    63721 tgattgtttc aaggaaacgt tgagcggcag ttccgttttt aacaccaacg gttttgccag
    63781 ttaattggtc gtacttgcta attttgtgtg actttgtagt agcaatgaca acttttgtat
    63841 catagtaagt atcagacatg gtgaagactt tttcacgttc tttagtcttt gtcatccctg
    63901 ccatgatagc gtcggcttgc ccagcttgaa ccgcattgac tgctgcgtca aatccaggat
    63961 aggacatctg aatgttccag cctttaatct cagcgacttt gttaataatg tcaacatcaa
    64021 ttcctttata agtttgatct gaatctttaa actcaaaagg tgcataggcg gtatcagaaa
    64081 caatcttaat cgtttctgct ttggcaatac ctaatgagaa aattgggaat aaaattagca
    64141 aaaatgctag aaattttttc ttcattttta gtctcctttt ccgaatattc tctcattata
    64201 tcagaaaaag taaaaaaagg caaattttta tatttttcgt gtcagaaaat ataacattga
    64261 tttttccttt tctttcttga attgctatta aaaagtgcta taataatagt atatatagaa
    64321 gaaaagagga cggggatatg aagaacaaaa gaatatttaa agacttccaa gcttcaaaaa
    64381 tgagtttaaa catttacaca agccccttgt tagcctttgt ttttgtcttc ataggagagt
    64441 ttgtggcttt tactttgtat ggtattggct tgttagctct catcggactt gctagaaatt
    64501 ttggagaggc tggtcaaaat cttgcaacct acttgcagac cttgcatcag agcttgacgg
    64561 ataaaacaag tgactttcgt ttaattttag gattactggc ctttggtttt attcttaaca
    64621 ctgtgttcag atggacaaga aaagttgaga aaagacctat tcgaaccttg ggattttata
    64681 gagagaattt cctcagcaat cttctgaaag gatttagtct aggcttggca ctttttcttc
    64741 tgaccttgtt aggtttagtg gtcttaggtc aatatcgttt ggaatccatt cacttgaatc
    64801 cttattctct tgcctttgtc gtctttacta tcccattttg gattttacag gggacagcag
    64861 aagaagtggt ggcccgtgct tggctacttc ctcaattggc ctcaagaacc aatctaaaac
    64921 tagctattct tatatctagc ctgttcttta ccctgcttca tatgggcaat tctggcctca
    64981 cccctctatc tctagtaaat ctctttttat tcggagttgc catggctctt tatcttctca
    65041 aaactgatac agtttggggt gttgcaggta ttcatggtgc ttggaatttt gctcagggta
    65101 atctctttgg gattttagtt agtggtcaac cgtcaggaac gtctctgatg acctttttac
    65161 cacaaggcaa tcaagattgg ctatcaggtg gttcttttgg catagaaggt tccattatga
    65221 caagtctggt attactactg ctgattgtct atcttgctaa taaattaaag aaagaaaatg
    65281 aaaggatgtg acttcggtcc gtccttttct tcgtgaaaat actataagta tgctaaaata
    65341 ggaatagcac atggagagag gattcttatg atcaatcaca ttacagataa tcaatttaaa
    65401 ctagtatcaa aatatcaacc atcaggagat caaccccaag ctatcgagca gttggtggat
    65461 aacattgagg ggggagaaaa agctcagatt ctgatggggg cgactggaac agggaagacc
    65521 tatactatga gtcaggtcat ttctaaagtc aataaaccaa ctctggttat tgcccacaat
    65581 aaaactctgg ctggtcagct ctatggggag tttaaggaat ttttccctga aaatgcagtt
    65641 gagtatttcg tatcctacta tgattattac cagccagagg cctatgtccc ttctagcgat
    65701 acctatattg agaaggatag ttctgtcaat gacgagattg acaagcttcg ccactcagct
    65761 acctcagccc ttttggagcg taatgatgtt attgtcgtgg cctcagtctc ttgtatctat
    65821 ggtttgggtt cgcccaagga atacgctgat agtgtcgtta gtctccgtcc tggtctagag
    65881 atttctcgtg ataaactctt gaatgacttg gtcgatattc agtttgaacg taatgatatt
    65941 gatttccaac gcggaagatt tcgcgttcgt ggggatgtgg tagagatttt cccagcttcc
    66001 cgagatgaac atgcctttcg agtagaattt tttggagacg aaattgaccg tatccgtgag
    66061 gttgaggctc tgacaggtca ggtattggga gaggtggatc atttggcgat tttcccagct
    66121 actcactttg tgaccaatga cgaccatatg gaagttgccg ttgccaagat tcaagcagaa
    66181 ttagaagagc agttagctgt ctttgaaaag gaaggtaaac tgcttgaagc ccagcgtttg
    66241 aaacagcgga cagagtatga tatcgaaatg ttgcgtgaga tgggctatac caatggggtt
    66301 gaaaattatt ctcgccacat ggatggacgg agcgaaggag agcctcctta tacgcttctc
    66361 gacttcttcc cagatgattt cttgattatg attgacgaga gtcatatgac catagggcaa
    66421 atcaagggca tgtacaatgg agaccgttcg cgtaaggaaa tgctagtaaa ttatggtttc
    66481 cgtttaccgt ctgccttgga caatcgtcct ctccgtcgtg aggagtttga aagccatgtg
    66541 catcagattg tttacgtatc agcgacacct ggtgactatg aaaatgaaca gactgagacg
    66601 gtgattgagc aaatcattcg accgacaggg cttttggatc ctgaggtgga agtccgtccg
    66661 actatgggac agattgatga tctcttaggt gaaatcaatg cccgtgttga aaagaatgaa
    66721 cgaaccttta tcaccacctt gaccaagaaa atggcagagg acttgaccga ctatttcaag
    66781 gaaatgggca tcaaggtcaa gtacatgcac tcggatatca agaccttgga acggacggag
    66841 attatccgtg atctccgctt gggtgtcttt gatgtcttgg ttggaatcaa tctccttcgt
    66901 gaagggattg acgttcctga ggttagcttg gttgccattc tcgatgctga caaggaaggt
    66961 ttccttcgta acgagcgtgg actcatccag accattgggc gtgccgcccg taacagtgaa
    67021 ggtcatgtca tcatgtatgc agatacggtt acccagtcta tgcaacgtgc tatcgatgaa
    67081 actgcccgcc gtcggaaaat tcagatggcc tataatgaag agcatggtat cgtaccacag
    67141 acaatcaaga aagaaatccg tgacctgatt gccgtgacca aggcagttgc taaggaagag
    67201 gacaaggaag tcgacatcaa tagcctcaac aaacaagagc gcaaagaact cgtcaagaaa
    67261 ctagaaaaac aaatgcaaga agccgtcgaa gtgcttgact ttgaactggc ggctcagatc
    67321 cgtgatatga tgttggaagt caaggccttg gattagggga atagtatgat ttatttaaga
    67381 aagttaaaga aagaagattt gatgtcttta tgggaaatgg cttattcaca gcttaatcca
    67441 gtttggaaac agtatgatgc tccctattat gatgattatc agtatttttc aaattttaaa
    67501 gaattcgaac tacaaaaatc agaatccatt ttaagcaact caaatcgcct tggtattttt
    67561 gttgatgata aactagttgg gactgtttcg cgttattggg tatgtaaaga aacaagatgg
    67621 atggaattgg gaattggtat ttatgataaa aaattctgga acactggtat tgggaaagtt
    67681 gctatgttgc agtggataga taggacgttt caggattact tggagttgga gcatctgggt
    67741 ttgacaactt ggtcagggaa tcttggtatg atgaaacttg ctgaaaaatt aagaatgaaa
    67801 aaagaagctc atattccaaa agttcgttat tatcaaggta aatattttga tagtattaaa
    67861 tatggtattt tgagagaaga ctgggagaaa ataaatgacg gttattatca aatcaatgga
    67921 aactcctgaa gagatagaag gtaaatccct cgttcactgg caaacgtgga gagaggctta
    67981 tgatgacctt ttgcctgcag aatttcagga gacaatgaca ttagaaagat gtcgattctt
    68041 tagtcaaaag tatccagaaa atacattgat tgcgataggt gatctgaagg tagttggttt
    68101 tatcagttat ggtaactttc gtgatgatac tattcaagct ggtggaatta ttgctttata
    68161 tgttttaaaa gactattacg gaaaaggcat agcacaaaag ttaatgaaag cagctttgac
    68221 tgctcttaat catttttctg aaattttctt atgggtattg aaagataaca agcgcgccat
    68281 tgctttctat caaaaaatgg gttttacttt tgatggacaa gaaaaaatac ttgaacttgg
    68341 aaagcctata aaggaaaaac gaatggtgtt ctattctaaa taattttaaa aaagtaaaag
    68401 ataatatctc tggtagtaag tctgaaaatt taataaatta gaaagcgagt aaatttatgt
    68461 cccgttccca attaacaatt ttaacaaata tctgtctgat tgaagacctc gaaacgcagc
    68521 gcgtggtgat gcagtatcgc gcccctgaaa acaatcgctg gtctggttat gcctttcctg
    68581 gaggtcatgt agaaaatgat gaggcttttg cggagtctgt cattcgtgaa atctacgaag
    68641 aaacagggtt gactatccaa aatcctcaac ttgtcggcat taaaaattgg ccactagata
    68701 caggtgggcg ctatattgtc atttgttata aggcgactga gttctctggt acccttcaat
    68761 cttcagaaga gggagaagtt tcttgggtgc aaaaagacca gattccaaac ttaaatctgg
    68821 cctatgatat gctaccattg atggaaatga tggaagctcc cgacaagtca gagtttttct
    68881 accctcgccg tacagaagac gattgggaaa agaaaatctt ctagtctttt actaaataac
    68941 ctagctgatc caaggcctcc tcgatatagt ggaggtcttg ttgtgtttcg gcttcgacta
    69001 ggtggtaatg gatgccatct gtcaattcag atagaggtct aaagtcagaa tgctcgactt
    69061 gttctagaaa atgttgcaca tctctgcgac aagacagttt aagcaaggtt tcaatttctc
    69121 cataaacggg atggtcaatc aaggtattct ggacatgccc accattatcg acaatagcaa
    69181 ggagttcctg accgatttct tcaacttcat gtttcacttt gaaaagtttg tgaacataag
    69241 gattggtatc aatttgctta tagatgtagc cacgattggt ggataggata ggagcgccat
    69301 cggctctcaa aattgcaatg tcctgcacaa tgacctgacg tgtgacatga aaatgttccg
    69361 ccaaactttg gccattaagg gctttaggag cttctttcaa gagttggagc agggcttgtt
    69421 tgcgatcttt tgtcatagct tttcctttta acggcgtttt cgaagcactt tatagacagc
    69481 tagtgctaat gtatagtcta ccatactatg gataattgtg ccaaatccaa ctagtacaaa
    69541 tagaacatag aacatgtttt ctacattggt acccgaagtt gcgtaaaaaa ggacacaggc
    69601 taatacttca gcaagggcat gaacaacagc caaaacaaaa ttgaaaaccc aggaagattt
    69661 tggtttatct agggtatcgg ggaatttttg taggtaaaga gctcctaaag caccaaaaga
    69721 tatatgggaa aaagcccgaa aaacgataac catgggatag ccagccatca aaaatccaaa
    69781 actagaggct aggatgacaa aaactgccat caagggcgac aagaacatgg ctataaaaat
    69841 agcgatgtgg ctccccaaag tataggaagc aggtggaatg acaatcttga aaggcataac
    69901 aattggaatc aaaatcgcaa tagccgttaa aagggctgtc attgtcataa attgtgtctt
    69961 tttccgtgta ttcacaagaa tctccttttt aactgcatat acactagtat ggtacaataa
    70021 accagacaat aaagcaagaa tttacttggg tttatagatc attttttagt taaaagttat
    70081 agtagattga aact
//
LOCUS       DAVBNO010000004        64454 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVBNO010000004 DAVBNO010000000
VERSION     DAVBNO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 64454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64454
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.004"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(1..1959)
                     /gene="ftsH"
                     /locus_tag="VQ846_000225"
     CDS             complement(1..1959)
                     /gene="ftsH"
                     /locus_tag="VQ846_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET3074880.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNREIHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(1975..2517)
                     /gene="hpt"
                     /locus_tag="VQ846_000226"
     CDS             complement(1975..2517)
                     /gene="hpt"
                     /locus_tag="VQ846_000226"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET3074881.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2521..3798)
                     /gene="tilS"
                     /locus_tag="VQ846_000227"
     CDS             complement(2521..3798)
                     /gene="tilS"
                     /locus_tag="VQ846_000227"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET3074882.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3795..5063)
                     /locus_tag="VQ846_000228"
     CDS             complement(3795..5063)
                     /locus_tag="VQ846_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET3074883.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5183..5551)
                     /locus_tag="VQ846_000229"
     CDS             complement(5183..5551)
                     /locus_tag="VQ846_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET3074884.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5544..5810)
                     /locus_tag="VQ846_000230"
     CDS             complement(5544..5810)
                     /locus_tag="VQ846_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET3074885.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(5868..9377)
                     /gene="mfd"
                     /locus_tag="VQ846_000231"
     CDS             complement(5868..9377)
                     /gene="mfd"
                     /locus_tag="VQ846_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET3074886.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYAQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            complement(9378..9947)
                     /locus_tag="VQ846_000232"
     CDS             complement(9378..9947)
                     /locus_tag="VQ846_000232"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET3074887.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            complement(10018..11133)
                     /gene="ychF"
                     /locus_tag="VQ846_000233"
     CDS             complement(10018..11133)
                     /gene="ychF"
                     /locus_tag="VQ846_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET3074888.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(11217..11411)
                     /locus_tag="VQ846_000234"
     CDS             complement(11217..11411)
                     /locus_tag="VQ846_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET3074889.1"
                     /translation="MYQVGNFVEMKKPHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11476..12612)
                     /gene="dnaN"
                     /locus_tag="VQ846_000235"
     CDS             complement(11476..12612)
                     /gene="dnaN"
                     /locus_tag="VQ846_000235"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET3074890.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12771..14132)
                     /gene="dnaA"
                     /locus_tag="VQ846_000236"
     CDS             complement(12771..14132)
                     /gene="dnaA"
                     /locus_tag="VQ846_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET3074891.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14345..15103)
                     /locus_tag="VQ846_000237"
     CDS             complement(14345..15103)
                     /locus_tag="VQ846_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET3074892.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15161..16342)
                     /locus_tag="VQ846_000238"
     CDS             complement(15161..16342)
                     /locus_tag="VQ846_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET3074893.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGKIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVIVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16525..17004
                     /gene="rlmH"
                     /locus_tag="VQ846_000239"
     CDS             16525..17004
                     /gene="rlmH"
                     /locus_tag="VQ846_000239"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET3074894.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17048..17121
                     /locus_tag="VQ846_000240"
     tRNA            17048..17121
                     /locus_tag="VQ846_000240"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17082..17084,aa:Arg,seq:tct)
     gene            17286..17411
                     /gene="comC"
                     /locus_tag="VQ846_000241"
     CDS             17286..17411
                     /gene="comC"
                     /locus_tag="VQ846_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET3074895.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            17432..18757
                     /locus_tag="VQ846_000242"
     CDS             17432..18757
                     /locus_tag="VQ846_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET3074896.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            18754..19506
                     /locus_tag="VQ846_000243"
     CDS             18754..19506
                     /locus_tag="VQ846_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3074897.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19558..19629
                     /locus_tag="VQ846_000244"
     tRNA            19558..19629
                     /locus_tag="VQ846_000244"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19591..19593,aa:Glu,seq:ttc)
     gene            19635..19708
                     /locus_tag="VQ846_000245"
     tRNA            19635..19708
                     /locus_tag="VQ846_000245"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19669..19671,aa:Asn,seq:gtt)
     gene            complement(19749..20291)
                     /locus_tag="VQ846_000246"
     CDS             complement(19749..20291)
                     /locus_tag="VQ846_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET3074898.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20470..21237
                     /locus_tag="VQ846_000247"
     CDS             20470..21237
                     /locus_tag="VQ846_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HET3074899.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21241..21660
                     /locus_tag="VQ846_000248"
                     /pseudo
     CDS             21241..21660
                     /locus_tag="VQ846_000248"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21688..22002
                     /locus_tag="VQ846_000249"
     CDS             21688..22002
                     /locus_tag="VQ846_000249"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HET3074900.1"
                     /translation="MAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMVTTLATW
                     NSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQTISINKS
                     FS"
     gene            complement(22103..24655)
                     /locus_tag="VQ846_000250"
     CDS             complement(22103..24655)
                     /locus_tag="VQ846_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET3074901.1"
                     /translation="MKSFLKTYRTYFISLIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDILAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(24717..26339)
                     /locus_tag="VQ846_000251"
     CDS             complement(24717..26339)
                     /locus_tag="VQ846_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET3074902.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            26545..27570
                     /gene="trpS"
                     /locus_tag="VQ846_000252"
     CDS             26545..27570
                     /gene="trpS"
                     /locus_tag="VQ846_000252"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET3074903.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            27722..29200
                     /gene="guaB"
                     /locus_tag="VQ846_000253"
     CDS             27722..29200
                     /gene="guaB"
                     /locus_tag="VQ846_000253"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET3074904.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(29251..30348)
                     /gene="recF"
                     /locus_tag="VQ846_000254"
     CDS             complement(29251..30348)
                     /gene="recF"
                     /locus_tag="VQ846_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET3074905.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKASVNGN"
     gene            complement(30351..30719)
                     /gene="yaaA"
                     /locus_tag="VQ846_000255"
     CDS             complement(30351..30719)
                     /gene="yaaA"
                     /locus_tag="VQ846_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HET3074906.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            30878..32128
                     /locus_tag="VQ846_000256"
     CDS             30878..32128
                     /locus_tag="VQ846_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET3074907.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAELEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            32125..33408
                     /locus_tag="VQ846_000257"
     CDS             32125..33408
                     /locus_tag="VQ846_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET3074908.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGYVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTIDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            33450..34271
                     /locus_tag="VQ846_000258"
     CDS             33450..34271
                     /locus_tag="VQ846_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET3074909.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            34282..34827
                     /gene="pgsA"
                     /locus_tag="VQ846_000259"
     CDS             34282..34827
                     /gene="pgsA"
                     /locus_tag="VQ846_000259"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET3074910.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            34824..35651
                     /locus_tag="VQ846_000260"
     CDS             34824..35651
                     /locus_tag="VQ846_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET3074911.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            35636..36475
                     /locus_tag="VQ846_000261"
     CDS             35636..36475
                     /locus_tag="VQ846_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET3074912.1"
                     /translation="MGIALENVNFIYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            36468..37262
                     /locus_tag="VQ846_000262"
     CDS             36468..37262
                     /locus_tag="VQ846_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET3074913.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            37321..38139
                     /gene="mreC"
                     /locus_tag="VQ846_000263"
     CDS             37321..38139
                     /gene="mreC"
                     /locus_tag="VQ846_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET3074914.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            38139..38633
                     /gene="mreD"
                     /locus_tag="VQ846_000264"
     CDS             38139..38633
                     /gene="mreD"
                     /locus_tag="VQ846_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET3074915.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            38727..39905
                     /locus_tag="VQ846_000265"
     CDS             38727..39905
                     /locus_tag="VQ846_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET3074916.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            40129..40908
                     /gene="rpsB"
                     /locus_tag="VQ846_000266"
     CDS             40129..40908
                     /gene="rpsB"
                     /locus_tag="VQ846_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET3074917.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            40987..42027
                     /locus_tag="VQ846_000267"
     CDS             40987..42027
                     /locus_tag="VQ846_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET3074918.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            42273..42560
                     /gene="tnpB"
                     /locus_tag="VQ846_000268"
     CDS             42273..42560
                     /gene="tnpB"
                     /locus_tag="VQ846_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET3074919.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALAPEQVD"
     gene            complement(42751..43019)
                     /locus_tag="VQ846_000269"
                     /pseudo
     CDS             complement(42751..43019)
                     /locus_tag="VQ846_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            43016..43414
                     /locus_tag="VQ846_000270"
     CDS             43016..43414
                     /locus_tag="VQ846_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3074920.1"
                     /translation="MDAPIPKAPLAHSFGSASIIAHIIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(43611..44531)
                     /gene="cysK"
                     /locus_tag="VQ846_000271"
     CDS             complement(43611..44531)
                     /gene="cysK"
                     /locus_tag="VQ846_000271"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET3074921.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(44816..45451)
                     /locus_tag="VQ846_000272"
     CDS             complement(44816..45451)
                     /locus_tag="VQ846_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET3074922.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            45507..46805
                     /locus_tag="VQ846_000273"
     CDS             45507..46805
                     /locus_tag="VQ846_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET3074923.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            46802..47464
                     /locus_tag="VQ846_000274"
     CDS             46802..47464
                     /locus_tag="VQ846_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET3074924.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            47544..48092
                     /gene="raiA"
                     /locus_tag="VQ846_000275"
     CDS             47544..48092
                     /gene="raiA"
                     /locus_tag="VQ846_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET3074925.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            48228..50201
                     /locus_tag="VQ846_000276"
     CDS             48228..50201
                     /locus_tag="VQ846_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET3074926.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            50198..50650
                     /gene="rplI"
                     /locus_tag="VQ846_000277"
     CDS             50198..50650
                     /gene="rplI"
                     /locus_tag="VQ846_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET3074927.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            50694..52046
                     /gene="dnaB"
                     /locus_tag="VQ846_000278"
     CDS             50694..52046
                     /gene="dnaB"
                     /locus_tag="VQ846_000278"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET3074928.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            52048..52314
                     /locus_tag="VQ846_000279"
     CDS             52048..52314
                     /locus_tag="VQ846_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074929.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            52403..53749
                     /gene="cbpD"
                     /locus_tag="VQ846_000280"
     CDS             52403..53749
                     /gene="cbpD"
                     /locus_tag="VQ846_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET3074930.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDSGSVGNSQSSASTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKIKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAINTTVDGYRVNSDGERV"
     regulatory      53832..53934
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            54005..54295
                     /locus_tag="VQ846_000281"
     CDS             54005..54295
                     /locus_tag="VQ846_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET3074931.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAVQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            54258..55016
                     /locus_tag="VQ846_000282"
     CDS             54258..55016
                     /locus_tag="VQ846_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3074932.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            55054..56061
                     /locus_tag="VQ846_000283"
     CDS             55054..56061
                     /locus_tag="VQ846_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET3074933.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            56061..56789
                     /locus_tag="VQ846_000284"
     CDS             56061..56789
                     /locus_tag="VQ846_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3074934.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            56908..57366
                     /locus_tag="VQ846_000285"
     CDS             56908..57366
                     /locus_tag="VQ846_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET3074935.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            57368..59800
                     /locus_tag="VQ846_000286"
     CDS             57368..59800
                     /locus_tag="VQ846_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET3074936.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYELDRLEEVALELTETDYSQDETFTELPFSRRLQILFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQACSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKVKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            60442..61095
                     /gene="rr06"
                     /locus_tag="VQ846_000287"
     CDS             60442..61095
                     /gene="rr06"
                     /locus_tag="VQ846_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET3074937.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            61092..62423
                     /gene="hk06"
                     /locus_tag="VQ846_000288"
     CDS             61092..62423
                     /gene="hk06"
                     /locus_tag="VQ846_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET3074938.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDIYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            62506..63033
                     /locus_tag="VQ846_000289"
     CDS             62506..63033
                     /locus_tag="VQ846_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET3074939.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAVVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            63112..>64454
                     /locus_tag="VQ846_000290"
     CDS             63112..>64454
                     /locus_tag="VQ846_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HET3074940.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLVMGSVVHATENEGST
                     QAATFSNMANKSQTEQGEINIERDKAKTAVSEYKEKKVSEIYTKLERDRHKDTVDLVN
                     KLQEIKNEYLNKIVQSTSKTEIQGLITTSRSKLDEAVSKYKKAPSSSSSSGSSTKPEA
                     SDTAKPNKPTELEKKVAEAEKKVEEAKKKAKDQKEEDYRNYPTITYKTLELEIAESDV
                     EVKKAELELVKEEAKEPRNEEKVKQAKAKVESEETEATRLEKIKTDRKKAEEEAKRKA
                     AEEDKVKEKPAEQPKPAPAPQPEKPAPKPENPAPKPENPAPKPEKPAEQQAEEDYARR
                     SEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYY
                     LNSNGAMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGSMATGWLQYNG"
BASE COUNT        20213 a        10986 c        12760 g        20495 t
ORIGIN      
        1 ttatttttcg tcattcattt ttgactttac ttcatcatag gatagtgcat gagattcctc
       61 ttctactgct tcaggcatct ttcctgtttc gtaaagagct ttaatttgtg tactatccaa
      121 tgtttcgtat ttcaataatg cttctgcaat taacttgtga atttcacgat ttgactgaat
      181 aatttcagca gctttatttc gtgcctcatt taataatgaa cgaacctctt catcaatttc
      241 ataagctgtt tgttctgaaa ttgatttttg aggactctgt gcaccaaaca tagcatggtt
      301 tccttcatat tgtactgggc caagtttttc actcataccg tactctgtaa ccattgcacg
      361 cgccatttgt gtcgcttgtt caaagtcgtt tgaagctcct gtagtttgga cattaaagat
      421 aatttcttca gctacacgtc cacccattaa gccagccaat tgctctttca tatcttcttt
      481 agatagaagc atttgatcct ctttaggaag tgcaatcatg tatccgcctg cacggccgcg
      541 tggtacaatt gtaaccttat ggacaacgcg agcattcgac aagactagac caacaatggt
      601 atgtcctgcc tcatggtaag caaccaattc tcgttctttt tgtgaaactg tcttatcttt
      661 cttagaaggt ccagcaataa ctctatcttc tgcttcatca atatctgaag catcaattat
      721 cgatttattg cgacgagcag caactaaagc tgcttcattc aagacattct ctaaatcagc
      781 accaacaaag cctggagttt gttgagccac taatttcaaa tcaacatctt ctgctaaagg
      841 cttgttctta gcgtgaactt tcaagattgc ttcacgacct ttaacatcag gacgaccaac
      901 caatactttt ctatcaaaac gtcctggacg caaaagggca ggatcaagta catctgaacg
      961 gtttgtcgca gcgatgacga taatcccttc atttccctca aaaccatcca tctcaatcaa
     1021 aagttggttc aaggtttgtt cacgttcgtc attacctccg ccgagaccga ctccacgttg
     1081 acgtccaaca gcatcaattt catcgataaa gatgatagct ggtgctgctt ttttggcatc
     1141 ctcaaaaaga gagcgaacac gactagctcc aactccgaca aacatttcta caaagtcaga
     1201 acctgagata ctaaagaatg gaacacctgc ttctccagcg actgccttag caagcaaagt
     1261 tttacctgtc cccggaggtc cctccaaaag aacacctgct ggaatacggg ctccaagttt
     1321 tgtgaatcgt tttggatctt ttaagaactc aacaacttca actagttctt gtttttcttc
     1381 ctcagctcca gcaacatctg aaaatcttac tttaatatct tctttatttg ctgctttagc
     1441 cttactacgt ccaaaactca ttggattacg gccattgcct cctcccatat ttcccatcat
     1501 agagaatagg aagaagaata gaattccaaa tggcacaatg gatacgagta gattaatcca
     1561 tataccactt gaactttcat gcttaacagt tacttctgct ttatggtcag tagcaagttt
     1621 ttgcaattct gatacggtag tatctgcagg aagaatagtg ctggtaaatt tctctacctt
     1681 agtaacagat ggcgtgaaaa actgaatacc tgtttcttct ttacttgttt taggattttt
     1741 atagacacca gaaacttcga taacactacc atttggttgg taagttaatt cttttacatt
     1801 accatcggta atttcttgta ccaactcagt atagttgatt tgctgacttc ctcctgagtt
     1861 attcccagaa tagaaatact ggaatcctgt cacaaggaaa aagataaata ataaccatag
     1921 aaaaggattt ttaattaaac cattattttg ttttttcatt aaagattatt ctttctaatt
     1981 tgaatacact tcctctttca atactccaat ataaggaaga ttacgataat tttctttgta
     2041 gtctaaacca taacctacta caaactcatt tgggatagta aagcaggtat agtctgcctc
     2101 aatttctaca acacgtcctt ctggtttatc caacaaggtt gcaattttaa cagaagctgc
     2161 ttctcttgct ttaaacatat ctcgcaaatt cttcaaagtt tgacctgtat caatgatatc
     2221 ttctacaaat agaacatgtc ttcctttgat atcttgagtc acatcttgtt taatattgat
     2281 aacaccacta cttgctgttc caccatggta gctagaaacc atcatgaagt ccatttcaat
     2341 atgtgtatca atatgtttga ccaattcagc cataaaagga atagatcctt ttaaaatccc
     2401 aactaagatt ggattttttc ctgcatagtc tttagttaat tgagcaccta gttttttagc
     2461 tgcttctgta atttcatcgt gtgaaacgag gactttttta atatcgtttt ctaacatttt
     2521 ttacctatct attttttcta tataaagtac agtgttcatt atatcatttt tcgttttttt
     2581 actcaaatta ttggtcgcaa ttcccaaaat tgagacaatt tcaccaaatt gctcaataat
     2641 aagagcagag tttctctttt ccatagggat tttcaaatca ataaataaac gtctgagttt
     2701 ttttctatgc ccattttgaa tcaaaacatc tcctgttttt cgatgacgaa tgtgtatgga
     2761 tgtttcacgt gaaacaggta tttgttgaat tgattcacct tctaatggaa gtccaaagga
     2821 aaataaatat ccttgataag ctacctgatt ttgatagtgt aacacaagtt catcttcctc
     2881 ttcatcagcc tgcggactga ttttacaaat ctgaaactgt tggtactctt ttatcaattc
     2941 atagccattt ttaatcggat gacgatactg gcttttagat tttaaaatct gctgaacttc
     3001 agcaaactga gcttttgtaa gattcaaatc tggaaaacga ttcagataag tttgaagtaa
     3061 aactctttgt gtagactcag agtaagaaaa taactgctgt aaatcttcca cattaatatt
     3121 gttagataat tcagctattg ccaaatcata atctaaaatt tcattgccaa tgcctaagat
     3181 tgcatcccta aatcgaggat tttctttttc caattctggt aagtaagagt ttcgaatacg
     3241 atttcgaaaa taatgattct cctgatttga tgtatcttca aagtgaaaaa ttgatggaaa
     3301 gtcttttttc tgaaaatgca agaagggacg aatgatttct atctctccga ctacttgctt
     3361 ctccttaatt cctgatagat agcgcaagcg agttcctcga atcaagcgca tcaaaatcgt
     3421 ttccacctga tcatcagcat ggtgggcagt gactaaagct gtcgcacctg tctttttcat
     3481 gacctcttga aaaaaatcat aacgaaaatt tcgtgcacgc gcttctgaaa attctcctga
     3541 aaaattgctg atataaatag gaagctctgc ttcagcagcc aacttcctta attccttttc
     3601 ttcccaatct gattcaattc tctgcttatg attcacatga gctagaatca attcaatctc
     3661 taactctttt tgataagtag acaatacctt aaatagaaac atggaatcta atccaccaga
     3721 aagagctaga accgccttag catgcttttt gaaatatccc ttcttgagaa aatgatttaa
     3781 aaaatctggt tccctcattt tagaacctca taaacatcct tggctatctt agaaatcgta
     3841 tcataatcag aattcttagt gaaaatagaa agaataaatg gagaatctgc atagacaaca
     3901 cccgtatcat gcttaaattc atccgcatct ccaattttat gagctacttt aacagaaaca
     3961 cctttggcaa ttcgctgact atcaaaatct gttttagtca aagactctaa cacaaatcca
     4021 ttttgattat aaatagcttc cataaacttc ccggccatct tagaagaaat caatttttct
     4081 tttggatccc aatcatctcc cataatggca gacatcttgg atttgaatgt ggcatcagat
     4141 tggtttgaaa tgtaatatcc caatagatta tgagctacat tatcagattc ttttgatact
     4201 ttcgtaatta aatcctttaa agaatattct ttattatctt cttttttagg aagactacca
     4261 cttccctctg gtttataaga acctggaaaa tcattgactg cagatacgta ttttacagtc
     4321 gtatctaact gataaagacc ctcatttatt ttttcttgcg tataatagag ataagagagt
     4381 ttcaaaacgc tggctgcata catcttttca tcttgattga taccagcttc ttttccagta
     4441 gtcagttgct taacataaat agagaaagaa tctttctgat atttttcaga taacatttct
     4501 tgaactttac tcatccgatt atcttcttca gaagttgatt ctttagctac ccatccagcc
     4561 tgatcaatat gtagaaattc tcttccttct acaaacatgg tcttgtcgat tgatacttgc
     4621 gaataagctg ataaggatga tttcacttct tttaaatcat aaggactatt gtacagttta
     4681 aagtcagatt ctaaccatgc tttttttatt gttggagtta cctctgattg atcatataaa
     4741 aatcgtttgt ccgcagctat aaattgatga tttgatagct taaatactgg aattccttgt
     4801 ttatttaagc gccactcggt tatttgaaaa cttgttttgg gagtcaattt cccagattcc
     4861 actactaaat cttcattcgc ataaacagga acctctccat aaaccatggg agaacttaat
     4921 ttttctctaa aataaatacc aaagtcagat tgtgaaaggt aataaatttc tttcgaagta
     4981 tagacgactt ctttttctgt gctaacgact tttgaaatgg tcaaaaaact tggtagcaac
     5041 aaaataatta agaatttacg cattcttcct ttccttttct tcttgtaaac gcaataattg
     5101 atttttatca gccaattctt ccagcttttc atctgacaaa ctcaaaaatt gctctattac
     5161 gtctaataaa ttttccattt tatcaccttt gaagcaagtc aggaatcgta taaacttttt
     5221 ccctcgactt agaataatag tacttcgctc gtgtatattt ggcagcataa tcttcatctt
     5281 tcaacttggt agcaaatgct gtctccttat ccttttcatc actcaaagtt tgatactgag
     5341 tttgcaagtc tgctaattgc tgacgtcttt ggagtaattg ctgataactc tgcgctaaat
     5401 taaaagttgg caagataaat aatagcataa tcaaaatcaa tacccctccc ataaaacgat
     5461 tccgtttttg tcgttctttc atcaggtagc gacgacgttg gtattcattt tgaataaaag
     5521 aattattcaa ttgtacaata tttttagaca ttttcttcta cccgtgtttc actgataatt
     5581 tcatacattc ctgctgcatc ttcttttttt gtactatctt tcatctctag tacttttaca
     5641 agcagcaact tattgccaaa gcgaatttca acttggtcat taactttcaa gtccgttgaa
     5701 cttttggcca agattccatt aaccttgatt ctacctttat ctgctacttc ctttgcgact
     5761 gtacgacgct tgataattcg cgatactttt aaatatttat ctaatctcat ccttattacc
     5821 tcaatttatt attgtaccat ttttatctat tttatagaag aaaaatatca aatggaattt
     5881 tcttccttag actcttttat ctctaataaa ctttctccaa aaatcagcag accttctaaa
     5941 atttcataat ctttcttatt ttggacatca aatacaagct ccattaatcc cttattctca
     6001 gcgatgcctg cttttaagtt cgttacggat aaagctttaa aataatcttg agctaaaaac
     6061 aatcgttgag tgactttttc aaattgaatt gtaattttat tatcttttct ttccacacgt
     6121 tgaacaaaga ccttgtccaa gtatgattta accaaaccaa tctctaaaag ataggctact
     6181 acatctgggt attctccaaa acggtctatc aactcctctt gtaactcttc ataattgaca
     6241 cggttgtcaa tttgacgaat tttcttgtaa atttcaatct tatgtcgttg atcagaaata
     6301 taagtatcag gaagataggc atcaatttgc aaaatcaact cagcattccc tttggttctt
     6361 gtgttagcgt tagcattacc gtttcgttta gcaatagctt cctctaataa ctgcgaatac
     6421 aattcaaaac caacagaatc aatgaaacca gactgggatt ttcctaaaag atttcctgct
     6481 ccacgaatcg aaagatctcg cattgcaatc ttaaagccag agcccaattc tgtaaatcct
     6541 ttaatcgctt ctaatctctt ttcagagact tcactgattg atttttctgg acgatacatg
     6601 agataagcat aagcaatacg attactacga ccgactcttc ctcttaactg atataaggtt
     6661 gacaagccca tatggtccgc attttcaata aataaagtat tagcatttgg aatgtccacc
     6721 cctgtctcaa taatagtagt cgtcaccaag atatcgtatt gtccctcaat aaagtctaat
     6781 agagtatttt ccaactggac ttcactcatt cgaccatgaa catatccaat cgaagcctct
     6841 ggaatcaact cctgtaattc tgaaaccttc tgaacaattg tatcaacttt gttgtaaaga
     6901 taataaactt gacctccacg ctccatttca cgcaagacag catcacgaat gacactatca
     6961 ttcttttcca aaacataggt ctgaacagga tagcgattag tcggcggagt ttcaataaca
     7021 gacaaatctc tgattcccag catagacata tggagggtac gagggattgg cgtagcggtc
     7081 aaggttagga catccacttg tttcttcagt tctttcaaag tttccttatg cttgacacca
     7141 aatcgctgtt cctcatcaat aatcatcaag cccaaatcag caaacacaac atcttttgac
     7201 aaaacacgat gtgttccaat caaaatatcg acttgaccgt ttttcaattt ttcaagtgtt
     7261 gcagtctgct cttttttact tctaaagcga ctcaacacat caacattaac tgcaaaattt
     7321 tggaatcgtt ccttaaaatt cgtatagtgc tgttgcgcta aaaccgtcgt cggaactaga
     7381 atgacaacct gtttgtgatc attgactgct ttaaaggctg cacgcatagc aacttcagtc
     7441 tttccaaaac caacatcccc aactaaaagt ctatccattg gctgagaagc ctgcatatcc
     7501 ctcttgattt cctcaatact acgaagttgg tcatccgttt caacataagg gaaagcatca
     7561 tcaaaggcat cttgatcatc atcatcagct gagaaagcaa aacccttcaa ctgactacgt
     7621 tcagagtaga gtttgattaa atcatcagct atatcctcta cctggttctt aaccttttgc
     7681 ttggcctttt taaaatgacc gtcatttaat ttattgagtt ttggagcttt accatcactt
     7741 gaaatatatt tggacagtag atgaatctgt tccacgggaa tagaaatttg atcaccattt
     7801 tggtattgga cactgacata atcacgatga attcccttga tttcaatggt ttcaattcct
     7861 agatattgac caatcccatg gatatgatgg acaacatagt cccctttttc aagttcattg
     7921 taatctttta atctctctgc atttgaaaca tgttgtcttc gaaaacgacg ctttaatttc
     7981 ttttgaaaaa tctcatgttc agttatcaat aaaatctttt catctacaaa atgaaaacca
     8041 tgtctgagat taccttctat taagtttaca gattctttac agatacttgt cttatctcta
     8101 gaatccaatt taatctgata ttcctctaac atatcctcca atgttttact tcccattgaa
     8161 ttgctagact gcagaataat ggtgtaatcc atttttttat atcgttcaat ttcttctttt
     8221 agaaaagaaa actgattgaa aaattcctgc ataggatatt gattgaattg ataaattttg
     8281 tcaaatttga gatttcctaa acccttttga agattagaga aaaaggtcac tggactttgt
     8341 tttttataga tttgttcaat atcagaaaaa tactgcatat cagaaaatgc tttactattc
     8401 tgtaattctt ctgtaaagta ctgcgctaat tctctttcaa agacttcata ctgattcatc
     8461 aatttttgat aatcatcaaa gaatattgga gtatcttttt caatataatc aaagacagtc
     8521 catgtcttat catagcacaa agataaaaac ttccgagaat ctgcatgact ttgcttttga
     8581 tgaaaacttg aaagaatttc ttctaggtat gatttcaaaa taggtgataa agtttttgaa
     8641 atttgttttt ctaaagctga ctgtcctcgt tgataatcct tttctctcaa aagcatatca
     8701 ctagctggaa agatagtgag ttctgtctta ttttctttcg ataattgtgt ttctacttca
     8761 aatgacctga taccatcaat ttcatcacca aaaaactcaa ttcgacaagg ttctaactgg
     8821 gatatttcaa aaatatctaa aatatctcct cgaagactaa attcaccctg agtttgtact
     8881 tgagtaactt ttcgatagcc attttccttt aactgatgga taaacgcgtg ttgagcatat
     8941 tcttcaccaa ctgagatttt tacaatacta tctttgaatg cattgggaga cggtaaaatc
     9001 aatcgacttg ctgcgatatt acaaactaaa atccctttct tagatgaatc agtcaaaaaa
     9061 cgcaaggctt caacccgtga aataattttt tcctgtgaag acatcaaaaa ctccaccata
     9121 ggagcatcat ctaccaaaaa tggatagacg agttcctcac ccaagataga aataagatca
     9181 ctaacaagtc cttctgcttc tccataagtt gacgtcaata acacaatcct atcttctttt
     9241 tctaaactgc ttgcaattgc aagagcctta gtagatgttg ataaaccaag tattagttgt
     9301 cttttcttat ctgttaaatt ttgatgccat tttttaatct gatcattttc tgagaataaa
     9361 tctaataagg tcaccattta tccgttatac ctctgcattg ttttctcaaa atttttctct
     9421 tgtaaatagt agtttacaga atcgtcaact ttgtcaacag actgtaaaat accgatataa
     9481 tcatccctgt caaacttact caaaacatga tgaacaactg acataccatt tttaggtctt
     9541 ccaattccaa tcttaacacg gttaaagacc tgagttccta tatgttgaat aatagacttg
     9601 ataccattat gaccacctgc tgagcctttt gctcttaaac gaattttccc aacttccatg
     9661 tcaagatcat cgtaaatgat aagtaaatcg tcaatatcca aaccatagta agttaataaa
     9721 gcatgaactg cttttccact ttcattcata aaggtcgttg gtttaaccag ataaattttt
     9781 tctccattta ggaaaaagga tgctaggtca gcttgaaata tcttatcgtg tgtaaaagtg
     9841 acattctgtt tcttcgctag ttgatcaatc aacataaaac caacattgtg ttttgtttca
     9901 aaatatttat cccctggatt tcccaagcct acaagtaatt tggtcattta tttttccttt
     9961 caaaagccaa aagggttgga atttttttcc aacctaattg acaccattta ttaattttta
    10021 gacattaaag cggaattcca tgatatcgcc atcttgaacg atatattctt ttccttcttc
    10081 acgcaagcgt ccagcttctt ttacggcctt ttcagatccg tatttcacta gatcttcata
    10141 tgacatggtt actgcacgaa taaagccttt ttcaaagtct gagtggataa taccagctgc
    10201 ttgaggagcc ttcataccac gtttgaaagt ccaagcgcga acttcttttt caccagctgt
    10261 gaagtaagtt cccaatccaa gcaagtggta agctgcacgc gtcaacttat ctacacctga
    10321 ttctgtcaaa ccaatggctt caagaaactc ttttttatct tcatcatcca attcagaaat
    10381 ttcttcctca gcacgcgcag aaataacgac tacttcagca ttttctgtcg ctgcaaattc
    10441 acgaatttgt ttgacatagt cgatagagtc aggttccgaa accacatcct catccacatt
    10501 agctacataa agaactggtt tagtcgtcaa aaggaaaaga cctttgacaa ccttttgttc
    10561 ctcatctgta aattcaatgg tacgagctga tttcccatct tctaggactg gtttaatctt
    10621 ttgaagaaca ttgaattctg ctactgattc tttatctttt tgcgtacgtg ccatcttttc
    10681 tacacgcgca tatcgtttgt tcactgattc taagtcagca agaatcaatt ccagattaat
    10741 ggtatcaata tctgcaagcg gatctacaaa ggcgtcttca cgtccttgct cgcgcatcac
    10801 attttcatca tcaaaagcac gaactacgtg aacaatcgca tctacttcac gaatattggc
    10861 caagaattta ttccctagcc cctctccttt tgaagctcct tttacaatcc ctgcaatatc
    10921 tgtaaattca aatgttgtgg gaactgtctt tttaggagtt atcatttcag ttagtttttg
    10981 tagacgttca tctggaactt ccaccattcc aacatttgga tcaatcgtcg caaatgggta
    11041 gtttgctgcc tctgctcctg cttttgtaat tgcattaaat agtgttgatt taccaacgtt
    11101 tggcaaacca acgatacctg ctgtcaaagc catatttctc attctccgtt ttcatttcaa
    11161 tccctaatat tataacacaa aaagggataa agtgctaacc ctctaactaa gggttctcag
    11221 tcaataattt tattcatttt tcgctcaaaa tcatatcgcc ccatcatgac aacatgctca
    11281 caattactgc attttatttt gatatctgct cctacacgtg taatttccca acgattagcc
    11341 tttttaccag tcgacttgat tgtacaagcg tgtggttttt tcatctcaac aaaatttcca
    11401 acttgataca tactactctc cttttctttt tcgattatat cataaaagag gcgagccagg
    11461 ctcaacctct ttcacttaat ttgtacgaac tggtgtaatg agctgcatga agtcttcgtc
    11521 agtatctgct ggcacaagag taaatggacg aacagctgag ataaagctaa tggtcacctt
    11581 ttcgctattt aaagctttaa gagaatcaat caagtaagtt gggttgaaac taatggtcaa
    11641 atcttcacca gtaacctgat cagtatcgat ttcttcgttt actttaccaa cttctggaga
    11701 gtgaacatgg gcgctaacaa ccccatcctt aatttcaagt ttcacagtac cattttgagt
    11761 cgcacttgat aaaagacggg cacgctccat tgactggcgt aagtttacca cattaaaagt
    11821 aatagtagtg ttaaagtctg ttggaatcaa gcgatctgta tcaggatagt ttccttctag
    11881 gagacgagta tagaagctaa tattttcgct tctaaagagg atttggttat tggcaaagaa
    11941 aatctctaca gtttcgatat catctgtaaa taccgctgaa aattcgcgta gagaacggct
    12001 aggaattacg acatcaaaat catcactatt tttttcaaga gtcaattttt tctggcttag
    12061 gcgatgagag tctgttgcaa ctgtttttaa ctctttgtgt tgactcaata cgaagtggac
    12121 acctgttaaa atcggacgac tctcttgtgt acttgcagca aaggctgttt cattaataat
    12181 tttcttgagt aattttgttt caagtattaa aggagtgctt gctgaaattt cttggattcg
    12241 tggatattgt tcgctatctt ttccttttag ggtaatttct gatttgccac tggttaaaac
    12301 aatttgattt tgttcaattt ctttaaaatc aagagttaca tcaggtaaac tagatactac
    12361 attgataaag aaagaagctt caagaaggat cgaacctaaa gaagtaatca acaaaccagc
    12421 atcttcattt ttttgagaaa taaaattttc aattgagatt tgaccatttg aaccaattaa
    12481 agtaatacct tcattggtca cgtcaatttt tactgttgat aaaataggaa tggcattttt
    12541 agaactaata gctctcttag tagtatttaa tgcttgtaga aataaatttt tattaattga
    12601 aaaatgaatc atggattctc ctttatttat ttttagtatt agaaagataa tagtaataat
    12661 agagtctgtg aaatctgtgg aaaacagcct ttaaacaagt cctatcaagt tttttagctt
    12721 gtttaaaaaa tgtggataaa aaagataaaa aactaaaagt tatccacaaa ttatttgatt
    12781 ttctttttga ttgattcaat ttctaaacgt aaattatcgt cttgatcaat caaagatttt
    12841 attttggcat gggcatgaat gactgtggta tgatcttttc ccccaaattc cttcccaatt
    12901 tttggaagac tattatctgt tagttctcta gataaataca tggctacttg acgggccaaa
    12961 acaatatttt gaaggcgtct acttcccttc atttctttga tactaacacc ataaaagtta
    13021 ccaacttcag tttggatttt atcaattggg atgacgagca tttggctaac atcttgtttg
    13081 cgggctctaa tggcttctgc agcaatatca atagtgatat ccttgatttt ttttactctg
    13141 gcaattaaag tgatgtcgtt gatggctccc tcaagatctc gaacatttga atcaaattgc
    13201 ccagctaggt attctagagt atcactttgg aaattgtagc ctaaatgttc cgttttactt
    13261 tgtaaaatgg caatacgtgt ttcaaagtca gggggtgtga tagtttgtgt caatccccaa
    13321 ctaaaacgcg tgacaagcct ctcctcgagc ccttctagat gttttggact acgatcactc
    13381 gttaggacaa tctgtttttg cttgtcatga agggcgttaa aggtattgaa aaattcttcc
    13441 tgagttgcga ctttttttcc gctgagtgac tggatatcat cgattaacaa aagatcaaga
    13501 ctacgatagg tctttttaaa cttttccatt tccccaagtc ttaggtgatc aagaaagtca
    13561 ttaataaagc tttcggcagg gatatattta acacgcgcat taggaatatt ttttagaatt
    13621 tcatttccaa tagcgtttaa taagtgagtc ttaccaaggc ctggtcctcc atagataaaa
    13681 agagggttat aggtcagagc caaatcttca gagacagcta aagcggctga tacagcccaa
    13741 acatttccat ccccttgaat aaagttatca aaggtatact tttcttttaa tcccgtatct
    13801 gaataaggaa tagatactaa ctttggacta tagtcataaa gagttaaatt tgtagcttct
    13861 tcaacttgtg agctagtcgt atcttgaggt ttggtgaaaa tatagtgggg agttatttca
    13921 gcgtcataaa tttcaaaacc agctactaca ataatatctt ttagttgttt ttcccagacc
    13981 atttccattt cagagcgagg tagaaatata gtggcaacat tttcctctac cttgataagt
    14041 tcagcttgaa tagcatagaa atcatacatg gatcgagtca gtctttcttg tgcaaattct
    14101 aatatacgat tccaaaattg tttttctttc aatcctttct cctcctttac tatttaaaag
    14161 tttaatacta gagatatttt accatagata gcaagaattt tccacaagct gtgaaaaata
    14221 atccacaatg ttaccaaact ttatccacag gttggggata aaagaagaaa ttattgattt
    14281 attaagcttt ttatcgaaaa acatagtgga taaccttgtg agataaaaaa ataaaagaac
    14341 agccttattt caggctgttg ataattctac tatattcttc ttgatttgaa aaagaaataa
    14401 tgatttttcc actgtctttt ttagatagtt taatttctac atctaatccg agtagttttc
    14461 ttaactgttt ttcttcattt tgtatgaaat gattagtttt ttgctgtttc ttttgttttt
    14521 tctctgtcag aagagcttct aatttcctta cagaaatatc ttcttctata atccgttgaa
    14581 agaaatagtc ttgttgttcc ttatttaacc caactaggga acgcgcatgg gcttgtgata
    14641 gtttgccatt ttctacttct gaaagaatct gttctggcaa ggaaagtaaa cgaatggagt
    14701 tgctgatata tggacgagac ttgcccatct tatctgcaat ttcagcatgg gtgaatcctt
    14761 tctctacgag agattcatag gcgcgtgctt cttctattgg gtttaaattt tctctctgta
    14821 aattttcaat aatggactgg accatcatct cttggtctga aatctgttta acaacagctg
    14881 ggatagaccg tagaccagct aaaagtgaag cccgatagcg tctctctcct gcaaggattt
    14941 cataaccaat aacaggagat tgacgaacaa taatcggttg aatgacccca ttttctttga
    15001 tagactgtgc tagttcatct agtttttctc tatcaaattc ttttcggggt tgatagggat
    15061 ttttttgtat atctgtgata gaaatcattt caaatttttc catgattcta cactaacaca
    15121 tcttttctct tatgtaaagc tttctttaca tagatgtcaa ttaagattct aaatcacctg
    15181 aactcttgtt aagtttgata gaggtagttt cttctttccc gttacgatag taggttatct
    15241 taatggtgtc tccgatagaa tggttgtaaa gagcactttg taagtctgtt gatgaagcaa
    15301 tctctttgtc atctactttt gtaattacat cgtatttttc aaggtgacca ttggcaggca
    15361 tattactttg taccgaacga acaattacac cagatgtaac attacttgga atattgagtc
    15421 ttctgatgtc gcttgtactc acattagata aattaaccat ctggattccc aaagctggac
    15481 gcgtcacttt tccgtttttt tctaactgtt caataatatt gatagcatca tttgcaggaa
    15541 ttgcgaaacc aagaccttct acagatgttc ctccatttgt agcaatttta cttgaggtaa
    15601 ttccgataac ctgcccttga atattgatca gtgggccgcc agagttacct gggttaatag
    15661 cagtatcagt ttggatggct tttgtagaaa tagcttgtcc atcttccgat tttaaggata
    15721 catttctatt gagactggat acgatacctt gagtgacagt atttgcatat tcagaaccta
    15781 acgggctacc gatggcaata gcagtttctc ctacagttaa cttactagaa tcaccaaact
    15841 cagctactgt tgtcactttt tctgaagaga ttttgacgac agcaatatca gagaaagtgt
    15901 cagctccgac aatttttcca ggtactttag tcccatctga caatcgaata tctactttgc
    15961 tggcgccatt tataacgtga ttgttggtga cgatgtaagc ttctttatca ttctttttat
    16021 aaataactcc agatccttca ctagagattc gctgagaatc tgtgtcagta tcatcattgc
    16081 caaatacgct attttgtctg tttgccgaat aagtaataac agaaacaaca gcatctttta
    16141 ctttgttaac agcctgtgtt gttgaatttt cgttcttata ggcagtttgt gtaatagtac
    16201 tattgttgtt agagttgttt acactacttt tttgagttag ttgagttatt gaaaaactac
    16261 ccaaggctcc actaaaaaag ctaatgacga taacgactaa taattgaaac cattttttgt
    16321 aaaatgtttt tagatgtttc atatttgcct ccatatgttt gaattactga aagtataaac
    16381 tgactagctt aattataact taaacacaaa agttttacac aaactgtgga taactctttt
    16441 gaaattgtga ttttcttaat tgaaatctat tttttatttt gtgaataaga tgtgaaaaaa
    16501 tagagaatat gttagaatag agtcatgaaa attaaagttg taacagttgg gaaactgaaa
    16561 gaaaagtatt taaaagatgg tatcgcagag tattcaaaac gaatttctag atttgctaag
    16621 tttgaaatga ttgagttatc agatgaaaaa acaccagata aggccagtga atcagaaaat
    16681 caaaagattt tagaaataga aggtcagaga attttatcaa aaattgctga ccgtgatttc
    16741 gttattgtgt tagccattga agggaaaact ttcttctcag aagaatttag taagcagtta
    16801 gaagaaactt ctataaaagg attttctact cttactttta ttattggggg aagtttagga
    16861 ttgtcatcat ctgtaaaaaa tagagccaat ctttctgtca gttttggtcg cctaacctta
    16921 ccccatcagt taatgagact agttcttgtt gaacaaatct atcgcgcttt tacgattcag
    16981 cagggattcc cctaccataa atagaaaatt gacttttaat tgaatttttg gtagaataat
    17041 tgtattaggt ctcatagctc agctggatag agcattcgcc ttctaagcga acggtcgcag
    17101 gttcgaatcc tgctgggatc aatataatag caaagctggg aattttcccg gcttttttct
    17161 taaaaaagta cactttggga gaaaaaaatg acagttgaga gaattttatc taaaacgaaa
    17221 ttccattttg tataatggtt tttgtaagtt agcttacaag aaaaaacatt ttaggagatt
    17281 ttattatgaa aaacacagtt aaattggaac agtttgtagc tttgaaggaa aaagacttac
    17341 aaaagattaa aggtggggag atgaggttgt caaaattctt ccgtgatttt attttacaaa
    17401 gaaaaaagta atgaaataag gggaaagagt aatggattta tttggatttg ggacggttat
    17461 tgttcatttt ttaattatta gtcacagtta ccattttatt tgtaaaggtc aaataaatag
    17521 aaaagaatta ttcgtttttg gtgcttatac attactaaca gaaatagtat ttgattttcc
    17581 cttatatatt ctatatttag atgggttagg gattgaaaga tttttatttc ctttgggatt
    17641 atattcctat tttcgatgga tgaaacagta tgagagggat agaggactat tcctaagttt
    17701 actactatct cttttatatg agagcactca taactttctg tccgtaactt tctcctctat
    17761 aacaggagat aattttgttt tacaatatca tttcccattc tttttcgttg tgacggtgtt
    17821 aacctatttt gttacattaa aaatcattta ctatttccat ttggaactag cctattttga
    17881 tgaggactac ctttatcctt tcttgaaaaa agtatttttt gctttactat tgctacatat
    17941 tgtatctttc gtttcagata tggtaagtac gattaaacat ttgaatagtt ttggaagtat
    18001 tttgtcatct attgtcttta tctctctcct tttgaccttc tttgcaatga attctcataa
    18061 agttcaaatg gagaaagaga ttgctttgaa gcagaagaaa tttgaacaga aacatttaca
    18121 gaattacaca gatgaaattg ttggtctgta taatgaaatc cgtggttttc gacatgatta
    18181 tgctggaatg cttgtcagca tgcagatggc aattgacagt ggtaatttac aggaaattga
    18241 cagaatttac aatgaagttt tagtcaaagc aaatcataaa ttgcgttcag ataagtacac
    18301 ttactttgat ttgaacaaca tagaagactc agctttacga agtttggttg ctcagtcaat
    18361 tgtctatgct cgaaataatg gtgtagagtt tacactggaa gtaaaagata cgattaccaa
    18421 gcttccaatt gaactattgg atttggttcg tatcatgagc gttttattga ataatgctgt
    18481 cgaaggatcg gctgatagct ataaaaagca gatggaagta gcagttatta agatggaaac
    18541 tgaaacagtt attgtgattc agaattcatg taaaatgacg atgactcctt caggagatct
    18601 atttgcctta ggattctcca ctaagggaag aaatcgcgga gtcggattaa ataatgtgaa
    18661 agaactacta gataagtaca acaatattat tttagaaaca gagatggaag gcagtacatt
    18721 tagacaaatc attagattta agagggaatt tgaatgaaag ttttaatttt agaagatgtt
    18781 attgaacatc aagtgagact agagagaata ttggatgaaa tttcgaaaga atcgaatatt
    18841 ccaatatcat acaagacaac gggaaaagtc cgtgaatttg aagaatacat tgaaaatgat
    18901 gaagtaaatc agctttattt cctagatatc gatattcatg gaattgagaa aaagggattt
    18961 gaagtggctc agctcattcg tcattacaat ccttacgcta ttatcgtctt tatcactagt
    19021 cgatcagagt ttgcgactct aacatataaa taccaggtat cagccctaga ttttgttgat
    19081 aaggatatca atgatgagat gtttaagaag agaattgagc aaaatatctt ctacacgaag
    19141 agtatgttac ttgaaaatga agatgttgta gattatttcg actacaatta caagggaaat
    19201 gatttaaaaa ttccttacca tgatattttg tatattgaaa caacaggggt atctcataaa
    19261 ttgcgcatta ttggtaagaa ttttgcaaaa gagttttatg gtaccatgac agatattcag
    19321 gaaaaggaca aacatactca gcgattttat tctcctcaca agtcattttt ggtaaatata
    19381 ggcaatatca gagaaattga tcgaaaaaac ttagaaattg ttttctatga agaccatcgt
    19441 tgtcctattt caagattaaa aattagaaaa ttaaaagata ttttagagaa aaaatctcaa
    19501 aagtgattga caattagcaa gaaattgata taatggttat atctgctaca gatagaaggt
    19561 ccgttggtca aggggttaag acaccgcctt ttcacggcgg taacacgggt tcgaatcccg
    19621 tacggactat tttatccgcc atagctcagt tggtagtagc gcatgactgt taatcatgat
    19681 gtcgtaggtt cgagtcctac tggcggagta tagataaaag ggaacacagc tgtgttcctc
    19741 tttttgtatc aatttgtatc accaagcatt ttcataagga agtctgttat ttcttgagaa
    19801 ctttcttttt ttccatgtgc aatccaagtt tggcagacac caaaaagtgc atgagttaga
    19861 tagatgctac tatattctaa ttcggtggta tttagattca gttgcataaa tcgcttttgt
    19921 aaatctgtac taagcatgat atgaagttta tttcgtaaga aattttggat ttctttagtc
    19981 ccattttcag aaagaagggc agccagaagt ggttctgact ctagatattc aaaaacttct
    20041 aaaatagcgt ctcttttgtg atgagcatgt ttttgaaaaa tatattcaaa tgtatggaat
    20101 agcttgcttt gatagtgctc aatcatatca tacttatcct tatagtgagt atagaagctg
    20161 gaacgactaa ttccggcttt ttctactaat ttgacagtag aaattttatc aaatggctgt
    20221 tccatcagta attgtaccat agcattttca atagttcgct ttgtttttaa gcgtttgtta
    20281 ctttcttgca tatttcctcc ttgtaaacaa attagactat atgtctaaaa atagattttt
    20341 tatcttgtaa tttagatttt ttaatgtata atctattata tcaaaatttt agacaatatg
    20401 tttaaaaaag gagaaactaa gtttaaagaa tggaaagcaa tttaaaaaaa accaaccttt
    20461 attattgtca tgatcgggat ttctcttatt ccagatctgt acaatatcat atttttgtca
    20521 tcaatgtggg atccatatgg gcaattgtct gacttacctg tggcagttgt aaataatgat
    20581 aaagaggctt cctataatgg taatactatg gcaataggaa aagacatggt gtccaattta
    20641 aaagaaaata aaaccttgga ttttcatttt gtagatgaag aggaaggaaa gaagggattg
    20701 gaagatggcg attactatat ggtagtgact ttaccaagtg atttatctga aaaaacaact
    20761 acattatcca atattcaatc gacagcagct tatcaatcat tgacaagtga gcaacaaact
    20821 gagataagtg attctgtatc tcaaaattca actgatagta ttcaatcggc tcagtcaatt
    20881 gtagctttag tacaagattt acagggaagt ttagaaaact tacaaaatca atcttctaat
    20941 ctttcgactt taaaaaatca atctaatcaa gtatcaccta ttacttctac ttctttgata
    21001 ggattgtcaa gtggattaac agagatacaa ggagatgtta ctagcaaatt agttcctgcc
    21061 agtcagtcga ttgcatcagg tgtaaacgca tatactacag gtgttgataa agtttctcag
    21121 ggcgcaagtc aactaagtga aaaaaatgcc accttgacag gtagtttgga taaactagtt
    21181 tcaggctcaa acaccttgac acaaaaatct tctagattga cagcaggagt tggttaatta
    21241 caatcaggat ctgggcaatt agcagacaaa tccagtcagt tactttcagg tgcttctcca
    21301 ttagagaata gagctaataa attggcagat ggatctagga aactagcaga aggtggaaca
    21361 aagttaactt ctggattgga agatttacag acaggacttg cttctttagg acaaggacta
    21421 ggtaatgcta gtgatcaact caaatcagta tcaacagaat ctaaaaatgc agagattttg
    21481 tcaaatccac tcaatctttc aaaaacagac aatgatcaag ttcctgtaaa tggaatcgca
    21541 atagctcctt atatgatatc agttgctctt ttttttgcag caatatcaac aaatatgata
    21601 tttgcgaaat tgccttcagg acgtcatcca gagagccgtt gggcttggtt gaaatcttga
    21661 gctgaaataa atggtattat agctgttttg gcaggaattt tggtatatgg aggagttcag
    21721 cttattggtt taactgctaa tcatgagatg agaatattta ttctcatcat cctaacaagt
    21781 ttagtattca tgtctatggt gaccacttta gcaacgtgga atagccgtat aggagctttt
    21841 ttctcactta ttttgctttt actacagtta gcatcaagtg caggtactta tccacttgct
    21901 ttgacaaatg atttctttag atctattaat ccctggttac caatgagcta ttcagtttcg
    21961 ggattacgac aaacaatctc tatcaacaag tcattttcct agctgtcata ctagctctat
    22021 ttactggttt aggtatgcta gcctatcaac ataagaaaat ggaagaagat taaaaaaatc
    22081 gaccgattaa ctggtcgatt ttttatgcct tagatgactt tcgtctgtga ttatagattc
    22141 caaatagtaa gagagaagta aaggaacaga ttgctccagt aataaaacca ttgggaatga
    22201 aggaaagtgt aatagttcct ttccccttgg gaatgtcaac tttcataaat ccagtttgag
    22261 cttgtttaat ttctattttc ttaccatctt ggtaggcaga ccaacctttg tcataaggaa
    22321 tggtgaagaa aatagatgta tcttgttgga catcatatgt agcaaaaacc ttgtttttag
    22381 aagttgatac tgtgacaggt tgttctttaa ttttttgaat tgcctcggtg aaagttttgg
    22441 tatctaaacg atagaaggta ggagattcaa atgatacttg tgaatttcca gggaaactaa
    22501 cattgatatt gaaagttttt ttctctttag tatatcctag attaaagaag gagaagacat
    22561 tatcagttgt aaaagtcttt ttttcaccat ttacaaggat gtcaaccttc ttttgtttat
    22621 cgttagaaaa gtgaaggttt atgaaagaga gataaacttg gctgttttct ggaacttcaa
    22681 tttgatactg gattgctgca tcttcatttg aagaacttgt gacactaatc aaatcattag
    22741 tattttctat tttttctgtt ttttcataag gtattggaga aaaataatca aaattgacgt
    22801 tagcaagttg atttaaaaat gaggcctgat tatccaaggt atgttcattg aacttgacat
    22861 cattgtaaac agattgacta gcaactgcaa tcggaagaga gtattgattt tcatataggg
    22921 taagattatc tttttgatag atatctttaa agccatactt atcaatagga ctgtctgaga
    22981 tattgtactg gataccaaat aaactatcag ccaaaatact attatttgca tatcggagat
    23041 tgagattagt cccagaggat ttaaaaccaa gtttatctaa agtagagctt gatgaacgat
    23101 ttcgaacaga tgaaaattga gagattccat tgtagttgaa tttcatactg tcatttcctg
    23161 tctgagtttg tagtttttca gtacgagtaa attgatttcc aatatatgtt gagaaagatt
    23221 ccatagctag gatatctcga ctataagcac ttcgagaagc aaatccccat tccttagcaa
    23281 ttccgtccat ttgagatgaa gcatttaaac tcatttcaac cagtataaat aaagagatta
    23341 gaatggcaaa tagattcaca gatataaact ttttgataac tgcaaggagt aaaagagaat
    23401 agacaaccaa aaattcaaga gtaagcagaa tattcaaatc tgttaaaaaa gaataatgcg
    23461 attttagata gatggtagct aaaaatcctg ctactacaag aaaaagcgaa actaaaaaat
    23521 tccagacttt aagttctttc agacgcttta agacttctgc tgctgtgtaa attaacaagg
    23581 tagagaaaat ccaagcatag cgatgtaaaa acatgtttgg agtatgcatg ccttgccaaa
    23641 ataagtcaag agcttctatg taaaagcttg caattagaaa tgcaaagaat attacatata
    23701 tgagtttcac gtgaaactta atagatttca gcgtaaaaaa taaaatggtc aaaataaagg
    23761 gaaatagtcc aacaaaaatc attgggatgg ccccatactt tgttgtgtca aaggaaccaa
    23821 tgaattgctt agcaaagaga tcaagatacc agctactttc agtttgaaac tttgtaactt
    23881 cagtcaattt ttccccatgt gtctgtaaat caaatagagt gggaagagtc ataatcaaac
    23941 tagccatacc agctaaaaag gagataacta tgaaatcaag aacagatgat tttcgagtct
    24001 taaagtccca cgaaatttga cagagatacc agaaaataag aaacaatact gtcatatatc
    24061 caaaataata attttgaata aataagattg acagacttgt aaagtacaat aggagtttct
    24121 tttcagttat cagtagatgt aaaccagtta taattaaagg aatcaagata aaaacatcta
    24181 gccaggtttt tatctctaat tgactgacag tgaaactcat cagagcatag gaagtagata
    24241 aggctagttt taaagtctga gggatagatt gaaacaattt attcaaacta aaaaaggttg
    24301 acagaccaat caatccaaat tttaagagag tggtcagata gacagcatct ggcatgttcg
    24361 ttagatcaaa aaagtaaact agaggcgcga gaaaactacc caagtaataa ctagataggg
    24421 catagaagtt tagccctaga ccacttgtaa aggtgtaaaa cagactacta tttccatgta
    24481 ggatatttcg taaggctaca tcaaaaataa cgtattgatg aaagccatct cctaatagag
    24541 gagagttgtc gctattccag tagatacctt gagatagata tactccagac ataatcacta
    24601 caggaatgat gagagaaata aaataggttc gatatgtttt taaaaatgat ttcatgttac
    24661 ctcgtagaat gatagaaaac tcagttggtt aacccaactg agttttgaag ttttatttag
    24721 tctttccaaa gttctttaac ttttgcttgt acttctgcat tttctaggaa ttcatcgtag
    24781 gtttcatcga tacggtcaat gacgccattt ttagataaga caatgatatg gttagccaaa
    24841 gtttgaataa attcgtggtc atggctagca aagatgattg attctttaaa gtttttcaat
    24901 ccatcattca agcttgagat agattccaag tccaagtgat ttgttggatc atcaagtaca
    24961 aggacatttg attttaagag catgagtttt gaaagcatga cacgaacttt ttctccccct
    25021 gacaagacat ttacaggttt gttaacttca tctccagaga agagcatacg gccgaggaag
    25081 ccacgtagga aagtattgtc atcttcttct ttacttgcga attgacgcaa ccagtcaaga
    25141 attgattctc ctcctgcaaa atcagctgag ttatcttttg gtaggtaaga ttgactagtt
    25201 gtaactcccc acttgacagt tccttcatag tcaatatctc ccatgattgc acgaattaat
    25261 gcagtcgttt gaatatcatt ttgtccaata agtgctgtct tatcatctgg acgcaagatg
    25321 aaactaatat tatccaagat agtttcacca tcaatcttta cagttagatt ttctactgtc
    25381 aagagatcat taccaatctc acgttccgct ttaaagttga taaatggata tttacgacta
    25441 gatggtacaa tctcttctag ctcaatctta tcaagcattt tcttacgtga tgttgcctgc
    25501 cttgacttag aagcattggc agagaaacga gcaacgaatt cttgcaattg tttaattttt
    25561 tcttctgctt tagcattacg gtctgctagc aatttagcag caagctcaga agattccttc
    25621 cagaagtcgt agtttccgac atagagtttg atttttccaa agtcaaggtc ggccatgtga
    25681 gtacaaactt tgtttaagaa gtgacggtcg tgggatacta cgataactgt gttatcaaag
    25741 tcaatcaaga agtcttctaa ccaagtaatc gattggatat ccaaaccgtt agtaggctcg
    25801 tccaagagaa gaacatctgg tttaccaaaa agtgctttgg cgaggagaac ctttactttt
    25861 tcaccgttgg ccaattcgct catgttttgg tagtgtaatt cttctggaat gtttaggttt
    25921 tgaagtagtt gagaggcttc actctctgct tcccaacctc caagttcggc aaactctcct
    25981 tcgagttcgg cagcacgaac cccgtcctcg tctgagaaat cttccttcat gtagatagca
    26041 tctttctctt tcatgatgct ataaagtttt tcatttccca tgataacgac atcaatggca
    26101 cgttcatctt cgtagtcaaa gtgattttga cgaagaacag agagacgttc atctggacca
    26161 agagagatgt gaccagtagt aggttcgata tctccagcta aaatttttaa aaaggttgat
    26221 tttccggcac cattagcacc gattaatccg taagtatttc cttctgtaaa tttgatattg
    26281 acatcatcaa aaagtttgcg atcactaaaa cgtagtgaaa catcagatac tgtaagcaat
    26341 atttttctcc tatatgtgta atatatttat tctactagaa aatacagaaa tattcaaatt
    26401 tttatttgtc aattttgtgt aaattatatt tacagtatcc tttacacaaa tctgtaaaaa
    26461 gcaaggctga tttattttga taaattacgg ttatttcatt aaaaaaatgc tataattgaa
    26521 aggactatat cgaaggagaa caaaatgact aaacccatta ttttaacagg agaccgtcca
    26581 acaggaaaat tgcatattgg acattatgtt ggaagtctca aaaatcgagt attattacag
    26641 gaagaggata agtatgatat gtttgtgttc ttggctgacc aacaagcctt gacagatcat
    26701 gccaaagatc ctcaaaccat tgtagagtct atcggaaatg tggctttgga ttatcttgca
    26761 gttggattgg atccaaataa gtcaactatt tttattcaaa gccagattcc agagttggct
    26821 gagttgtcta tgtattatat gaatctagtt tcgttagcac gtttggagcg aaatccaaca
    26881 gtcaagacag agatttctca gaaaggattt ggagaaagca ttccgacagg attcttggtc
    26941 tatccaatcg ctcaagcagc tgatatcaca gctttcaagg ctaattatgt tcctgttggg
    27001 acagatcaga aaccaatgat tgagcaaact cgtgaaattg ttcgttcttt taacaatgca
    27061 tataactgtg atgtcttggt agagccggaa ggtatttatc cagaaaatga gagagcaggg
    27121 cgtttgcctg gtttagatgg aaatgctaaa atgtctaaat cactaaataa tggtatttat
    27181 ttagctgatg atgcggatac tttgcgtaaa aaagtaatga gtatgtatac agatccagat
    27241 catatccgcg ttgaggatcc aggtaagatt gagggaaata tggttttcca ttatctagat
    27301 gtttttggtc gtccagaaga tgctcaagaa attgctgata tgaaagaacg ttatcaacga
    27361 ggtggtcttg gtgatgtgaa gaccaagcgt tatctacttg aaatattaga acgtgaactg
    27421 ggtcctattc gtgagcgccg tattgaattt gctaaggata tgggagaagt ttataatatg
    27481 attcaaaaag gtagtgaaag agcgcgtgaa gttgcgggtc aaaccctatc tgaggtaaaa
    27541 ggggcaatgg gacttcatta ctttaactaa gtttatttta caagaaacta tcatttctat
    27601 ctcgaagaaa agatagtttt ttatttaccc ctgtaacatt tgacaaattt ttatagaatg
    27661 gtatgataga tacaatataa aaagagtcaa gctcaaaaag aaagaaaaga ggaaacttcg
    27721 aatgtctaat tgggacacta aatttttgaa aaaaggtttt acctttgatg atgtattgct
    27781 tattccagct gaaagtcatg tgttgcctaa cgatgcagat ttaacaacta aattggcaga
    27841 taatttgact ttaaatatcc caattattac cgctgccatg gacacagtta cagagagtca
    27901 aatggccatt gctattgctc gtgcaggtgg tctcggagtt atccataaaa acatgtcaat
    27961 tgctcaacaa gcagacgagg ttcgtaaggt aaaacgttct gaaaatggag ttattattga
    28021 tccgttcttc ttgacgcctg aacatacaat tgctgaagca gatgagctta tgggtcgtta
    28081 ccgcatcagt ggtgttccag ttgttgaaac acttgaaaat cgtaaattgg ttggtatttt
    28141 gacaaaccga gatcttcgtt ttatttcaga ttataatcaa ccaatttcaa accatatgac
    28201 tagtgaaaat cttgttactg ctcctgtggg tacggatctt gcaacggctg agagtattct
    28261 tcaagagcat cgtattgaaa aacttccgtt ggtcgatgaa gaaggcagtc tttctggttt
    28321 gatcactatc aaagatattg aaaaagttat tgagtttcca aatgcggcta aagatgaatt
    28381 tggtcgtctt ctagttgcag gtgcagtagg tgttacttca gatacatttg aacgtgcaga
    28441 ggctcttttt gaggcaggag cggatgcgat tgttattgat actgcacatg gtcattctgc
    28501 aggtgtcttg cgtaaaattg ccgagattcg tgctcatttc ccagatcgga ctttgattgc
    28561 tggaaatatt gctactgctg aaggtgcacg tgccctttat gaagcgggtg tagacgttgt
    28621 taaggttggt attggaccag gttctatctg tactactcgt gtgattgctg gtgttggtgt
    28681 tccgcaagta acagctatct acgatgctgc agctgttgcg cgcgaatatg gtaaaacgat
    28741 tattgctgac ggtgggatca agtattctgg agatattgta aaagcacttg ctgcaggtgg
    28801 aaatgctgtt atgcttggat ctatgtttgc tggaactgat gaagctccag gcgaaactga
    28861 aatcttccaa ggacgtaaat tcaagactta ccgtggtatg ggatcaattg ctgctatgaa
    28921 gaaaggttca agcgaccgtt atttccaagg ttctgtcaat gaagcaaaca agctcgttcc
    28981 agaaggaatt gaaggtcgtg ttgcttataa aggagcggca gctgatattg ttttccaaat
    29041 gattggtggt attcgctctg gtatgggtta ctgtggtgca gctaacctta aagaactaca
    29101 cgataatgct caatttattg aaatgtctgg tgctggtttg aaagaaagcc atcctcatga
    29161 tgtgcaaatt actaatgagg caccaaatta ttctatgtaa aaaacaatga aaagaactcc
    29221 agtgaaaaca ggagttcttt tacaatgttg tcaatttcca tttacagaag ctttaccatc
    29281 ctgaatagtg aagatactta gattttctgg cagattttga agatggtcta agcttgttgt
    29341 tgtgataaag gtttggattg attgagaaat cgtttctaat aattttaact gtctagtgtt
    29401 gtcaagttca ctcatcacat cgtcaagcag taatatagga gattctgtgg taatgctttc
    29461 cattaattcg atttctgcta attttatcga gaggacgaga ctacgatgtt gaccttggct
    29521 tccgaaacta gcatccatcc catttatata aaaagaaatg tcatctcgat gaggaccgac
    29581 accagtattc tttttaaata aatctctgga tctacttttt tctaaagcaa ttttgaaaga
    29641 ttcggataag ttttgtttgt cagttatatt gacagaagat tgataggata ttgacaactc
    29701 ttcgatctga ttagagagtt caaaatgttt cttacgccca aatgattcta gtttttttat
    29761 gaaatctaag cggtgattca ttacacgaca tccataatca actagctgat catctaacac
    29821 agaaaggaat gtttcatcta ttttttgagc tgattttagg taagtgtttc tttgctttag
    29881 gatgtggtta taattggtta agtcagataa atagattggc ttaatttgcc caagttccat
    29941 atcaatgaat tttcgtcgaa tcgaaggtgc tcctttaatt agttgtaaat cttcaggagc
    30001 aaataagaca acattcatgt gtcctacata atctgaaagg cgtgcctgtt ttaagtgatt
    30061 aacttttgtt acacggcctt tttgtgttag ttcgatttct aggggaatgg atccagtttt
    30121 tttctgaacg agacctgaaa gatgaagttg ttcctcatca aaatgaatga gatttttatc
    30181 tgttcgagtt cgatgactac gcgttaaggc taaaaaatag atagcctcta acatgtttgt
    30241 ttttccttgt gcattacgtc ccaaaaagac attcaattta ggattaaagt ctattttcgt
    30301 ttctttgtaa ttacgaaaag tcttgagaga tagatgttgt agccacatga ttatctacca
    30361 gggaatcgtg gagcttgttg gcttttaggt gatgaagcag gtttcgattt gtctttttta
    30421 actcccttat tcatcttttt gacaagttta gcgatccgtt ctttttcaac tttatcagct
    30481 tggtattcat cttgttcttc agaagtaggt tgtgtcaaca agatgtcaat gttcatgtca
    30541 gggatgtcaa ctttatcgcc aatacgaagt ttttttccac gacgactctc taattcccta
    30601 ttaaagtaaa cagaatgttc agagagaaat gatttaatag ctcctccgct atgtgtaatt
    30661 ccaagttctt tgagtagtgc ttggagggta ataaattctt caaataattt gtattccata
    30721 attcacctca atctttggta ttataccata ttttcctaaa aatagtgaga gagacagcga
    30781 gaaatgtaag cgatttttat ttcaaaagtg ttacagggaa caagaaaatt tcaggttttc
    30841 gtgatataat agaagtctgt atataaggag gtaaatcatg gagttagtgc atggaatttc
    30901 aacacatttt atccaatcaa aaaagtttaa aacaaacaaa attaccgtgc gttttaccgc
    30961 tccattatcc cttgatacga ttgcaggtca catgttgagt gcaagtatgc tagagactgc
    31021 taatcagatg taccccactt ctcaagattt gaggagacac ttggccagtc tatacggtac
    31081 agatatgtca accaattgtt tcagaagagg gcaaagccac attatagaat tgacatttac
    31141 ctatgttcgt gatgagtttt taagtaggaa aaacgtgcta acctctcaga ttttggaact
    31201 tgtaaaagaa actctttttt cacccgcagt agttgataat gggtttgatc cggccttatt
    31261 tgaaattgag aaaaaacaat tgctagcaag tttagcagct gatatggatg attcttttta
    31321 ttttgcacat aaagaattgg ataaattgtt ttttcatgat gaacgtcttc aattggaata
    31381 tagtgattta cgaaatcgta ttttagctga aactccacaa agttcttatt cttgtttcca
    31441 agaattttta gccaatgatc gaatagattt ctttttccta ggtgatttta atgaggttga
    31501 aattcaaaat gtattagaat catttggctt taaaggtcga aaaggagatg tgaaggttca
    31561 gtattgtcaa ccttattcta atatccttca ggaaggtatg gttcggaaaa atgtgggaca
    31621 atccattttg gaattaggtt atcattaccg ttctaaatat ggtgatgagc aacatttacc
    31681 catgattgta atgaatggtt tacttggtgg atttgctcac tctaagctct ttacaaatgt
    31741 ccgtgaaaat gctggattag cttataccat ttcaagtgag cttgatttat ttagtggatt
    31801 cttgaggatg tatgctggta tcaatcgaga aaatcgtaac caggctcgta aaatgatgaa
    31861 taatcaactg cttgatttaa aaaaaggtta ttttacagag tttgagttaa atcagaccaa
    31921 ggaaatgatt cgttggtcgt tgttactttc tcaagataat caatcttcat tgattgaacg
    31981 tgcttatcaa aatgccttat ttggaaaatc ttcagcagac tttaaaagtt ggattgcaga
    32041 gcttgaacaa attgacaaag atgctatttg tagagtagct aataatgtga aactacaagc
    32101 gatttacttt atggaaggaa tagaatgaca aaggttgttt ttgaagaaaa atactatcca
    32161 gctgtaaaag aaaaggttta tcgaactcgt ttggccaacg gattgacagt tgctcttttg
    32221 cctaaaaagg aatttaaaga ggtttacggg agtgtcactg tacagtttgg ttcggtagat
    32281 acgtttgtca cagaagttga cggatatgta aaacaatatc ctggaggaat tgctcatttt
    32341 cttgaacata aattatttga gagagaagat tctagtgatt tgatgtcggc ttttacgagt
    32401 ctaggtgcag atagtaatgc ctttacaagc tttacaaaaa caaactatct tttttcagca
    32461 acggattatt ttttagaaaa tttagattta cttgatgaat tggtaacatc agcacacttt
    32521 actgaagctt ccattctgac agagcaggat attattcagc aagaacgaga aatgtaccaa
    32581 gatgatccag attcgtgttt attcttttca actttagcga atttgtatcc tggtacacct
    32641 ttagcaactg atatagttgg aagtgaggag tccatttccc aaatcaatct aactaatttg
    32701 caagaaaatt ttacaaagtt ttacaaacct gtaaacatgt ctctgttttt agttggtaat
    32761 tttgatgtgg agcgagtaca ggactatttt gaaagcaaag aactgaaaga ttcagatttt
    32821 caggaagtag caagagaaaa gttgttttta cagcctgtaa agccaacaga tagtatgaga
    32881 atggaagtat cttctcccaa actagcgatt ggagttagag gtaagcgaga agtttctgaa
    32941 gcggattgct atcgacatca tattttatta aaattattgt ttgcaatgat gtttggttgg
    33001 acttcggatc gttttcaaaa atgttatgaa tcaggtaaaa ttgatgcgtc cttatctctg
    33061 gaagttgaaa taacaagtcg ctttcatttt gtcatgttga caatagatac gaaagagcca
    33121 gttgctttgt ctcatcaatt taggaaggct attcgtaatt ttacaaagga tttagatatt
    33181 acagaggaac atttagatat tatcaaaaga gagatgtttg gcgaattttt cagtagcatg
    33241 aactctcttg aatttattgc aacgcaatat gatgcttttg aaaatggtga gacaattttt
    33301 gatttgccga aaattttaca ggaaattact ttagaggatg tccttgatgc tggacatcat
    33361 ttaatagatg atggtgacat agttgatttt acaatattcc catcgtagta acctatcata
    33421 atagacacta gaaagaaggg atgacaagta tgagaaaaaa aacaattgga gaggttttac
    33481 gattagctag aatcaatcag ggattgagtt tagatgaatt gcagaaaaag acagaaatcc
    33541 agttagatat gttggaagca atggaagcag acgatttcga tcaacttcca agtccttttt
    33601 acacgcgttc tttcttgaaa aaatatgcat gggctgttga gttagatgac caaattgttt
    33661 tggatgctta tgattctggg agtatgatta cttatgagga agtagatgtt gatgaagatg
    33721 agttgacagg tcgtagacgt tcaagtaaga aaaagaagaa aaaaacatca tttttacctt
    33781 tattttattt tatccttttt gctttatcga ttttaatttt tgtgacttat tatgtttgga
    33841 actatattca aactcaacca gaggagcctt ctctttctaa ttacagtgtg gttcaatcaa
    33901 caagttcaac tagctctgtt ccccactcct caagtagtag ttcttctagt atagaatcag
    33961 ctataagtgt atcaggcgaa ggaaatcatg tagaaatcgc ttataagaca agtaaggaaa
    34021 cagttaaatt gcaattggca gtttcagatg ttacaagttg ggtcagtgtt tcagaaagcg
    34081 aacttgaggg cggtgtaacc ttatcgccaa agaagaaaag tgcagaagca acagttgcaa
    34141 ctaaaagtcc tgtaacaatt acgttaggtg ttgtaaaagg tgttgatttg acagtagata
    34201 atcagactgt tgatttatcg aaattaacag ctcagactgg acaaatcact gtaaccttta
    34261 ctaaaaatta aggaaaaacg aatgaaaaaa gaacaaattc ccaatctctt aacaataggt
    34321 cgaattctct ttatacctat ttttatcttt attttaacga taggaaattc gatagagagt
    34381 catatagttg cagctattat ctttgctgtt gccagtatta ccgactattt agatggatat
    34441 ttagctcgta aatggaatgt ggtcagtaat tttggtaaat ttgcagatcc tatggcggat
    34501 aagttactag ttatgtcggc ttttattatg ttgattgagt taggtatggc tccggcttgg
    34561 attgttgcag tgattatctg tcgtgagtta gctgtgacag gtttaaggct tttattggtt
    34621 gaaactggtg gaacaatttt agcagcagca atgcctggaa aaattaaaac ttttagtcag
    34681 atgtttgcta ttattttctt gctattacat tggactttgc ttggtcaagt tctactttat
    34741 gtagccttat ttttcactat ctactctggc tatgactatt tcaagggtag tgcctatgta
    34801 tttaaaggga catttggttc gaaatgaaat caataattga tgtaaaaaat ctttcttttc
    34861 gctataaaga aaatcagaac tactacgatg tgaaggatat tacgtttcac gtgaaacgtg
    34921 gagaatggct ttcgattgta gggcataatg gtagtggtaa atcaacgacg gttcgattaa
    34981 ttgatggctt actggaagca gaatccggag agattgtaat tgatggccaa cgtttgactg
    35041 aggaaaatgt ttggaatata cgtcgtcaaa tcggtatggt ttttcaaaat ccagacaatc
    35101 aatttgttgg agcgactgtt gaagatgatg ttgcttttgg tttggaaaat cagggacttt
    35161 ctcgtcaaga aatgaaaaag agagtggaag aagctctggc tttagttggc atgttggact
    35221 ttaaaaagag agagccagcg cgtctatcag gtggccaaaa gcaacgtgtg gccattgcag
    35281 gtgttgtagc cctaagacca gctattttaa tcttagatga agcaacgagt atgttggatc
    35341 ctgaggggcg tagagaactt attgggacag taaaaggaat tcgaaaagac tatgatatga
    35401 cggtcatttc tattacccat gatttggaag aagtcgccat gagtgatcgc gtattggtca
    35461 tgaaaaaagg ggaaattgaa tcaactagta gtccaaggga gcttttctct cgaaatgatt
    35521 tagatcaaat tggattagac gatccttttg ctaatcaatt aaaaaaatct ttgagccaga
    35581 atggctatga tttacctgaa aattatttga cagaaagtga gctagaggat aagctatggg
    35641 aattgctcta gaaaatgtga attttatata tcaagaaggt actcccttag cttcagcagc
    35701 tttgtcggat gtttctttga cgattgaaga tggctcttat acagctttaa ttgggcacac
    35761 aggtagtggt aaatcaacta ttttacaact cttaaatggt ttattggtgc caagtcaagg
    35821 gagtgtgagg gtttttgata ccttaatcac ctcgacttct aaaaataaag atattcgtca
    35881 aattagaaaa caggttggct tggtatttca gtttgctgaa aatcagattt ttgaagaaac
    35941 ggttttgaag gacgttgctt ttggaccgca aaattttgga gtttctgaag aagatgctgt
    36001 gaagactgcg cgtgagaaac tggctctggt tggaattgat gaatcacttt ttgatcgtag
    36061 tccgtttgag ctgtcagggg gacaaatgag acgtgttgcc attgcaggca tacttgccat
    36121 ggagccatct atattagtct tagatgagcc aacagctggt ctagatcctc tagggagaaa
    36181 agagttgatg accctgttca aaaaactcca ccagtcaggg atgaccatcg tcttggtaac
    36241 gcatttgatg gatgatgttg ctgaatatgc taatcaagtc tatgtaatgg aaaagggacg
    36301 tttagtaaag gggggcaaac caagtgatgt ctttcaagac gttgttttta tggaagaagt
    36361 tcagttggga gtacctaaaa ttacggcctt ttgtaaacga ttggctgata gaggcgtgtc
    36421 atttaaacga ttaccgatta agatagagga gttcaaggag tcgctaaatg gatagtatga
    36481 ttttggggcg ttatatccca ggggattcga ttgttcaccg attggatcca cgtagcaaat
    36541 tgcttgctat gatgctactg attttgattg tgttttgggc taataacccc ttgacgaatc
    36601 tgattctttt tatagcgaca gggatattta ttgccttgtc aggagtatct ctttcatttt
    36661 ttattcaggg cttgaagtct atgtttttct tgattgcctt cacaactatt tttcaactat
    36721 ttttcatttc taatgggaat gttttatttg agttttcgtt tgtgagaatc acggattatg
    36781 ctttgcaaca agctgggatt attttttgtc gctttgtatt gattattttc ttttcaactt
    36841 tgttaacctt aacaaccatg cctttaagtt tggcatcagc tgtcgaagct ttattagcac
    36901 ctttaaagcg tgtgaaagtt ccagttcatg aaattggatt gatgctgtct atgagtttgc
    36961 gttttgtccc aaccttgatg gatgatacga ctcggattat gaatgcacag aaagctcgtg
    37021 gagtggattt tggagaagga agcatcgttc aaaaagtaaa ggcgatgatt cccattttga
    37081 ttcctctttt tgcgacaagt ttaaaacgtg cagattcctt ggctatcgct atggaagcgc
    37141 gtggctatca gggtggaaaa ggcagaagtc aataccgaca attgaaatgg actctaaagg
    37201 atacgctgac cattcttgtt attctcgtac ttggttgttg tttatttttc ttaaaatctt
    37261 agtagctttc aggaattgat aaaaagttac tgtaacagtt tttgatataa aggtagggat
    37321 atgaaccgtt ttaaaaaatc aaaatatgtc attattgttt ttgtcactgt tctgcttgtg
    37381 tcagctctct tagcgacgac ttattcaagt acaattgtga caaaattagg agatggaatc
    37441 tcattggttg atagagttgt acaaaaacct tttcagtggt ttgattctgt caaatcagat
    37501 ttggctcatt tgacacgaac atataatgaa aatgaaagtt tgaagaaaca gctttaccaa
    37561 ttagaagtta aatcaaatga ggtggaaagt ttaaagacag aaaatgaaca actgcgccaa
    37621 ttgcttgata tgaagtctaa attgcaagcc acaaagactt tagcagcaga tgttattatg
    37681 cgttctccgg tatcttggaa gcaggagttg accttagatg caggtagatc aaaaggtgct
    37741 tctgagaaca tgttagctat tgcaaatggt ggcttgattg ggagtgtttc aaaagtagag
    37801 gagaactcta ctatagtcaa ccttctgaca aatacggaaa atgctgataa gatttctgtt
    37861 aaaattcaac atggctctac tacaatttat ggaattatta ttggctatga caaggaaaat
    37921 gacgttctta aaattagcca attaaatagt aatagcgata ttagtgcggg agataaggtg
    37981 actactggtg gattaggaaa ctttaacgtt gctgatattc ctgttggtga agtggttgcc
    38041 acaacgcata gtacagacta tttgacacga gaagtaactg ttaaattgag tgcagatact
    38101 cataatgtag atgtgataga attagtgggg aattcataat gagacagttg aagcgagttg
    38161 gagtattttt attgcttcct ttctttgttc taattgacgc ccatattagc cagcttctgg
    38221 gctcattttt cccccatgta catttggcta gtcattttct ttttctattt ctcttatttg
    38281 agacgataga agtatcagag tatctctacc tagtctattg ttttgttata ggcttggttt
    38341 atgatgttta ctttttccat ctaataggga ttacaactct cttatttatc ttattgggag
    38401 ccttccttca taaattgaat agtgttattt tgttgaatcg ttggacaaga atgctagcta
    38461 tgattgtgct gacattcctg tttgaaatgg gtagttatct tttggctttt atggtagggt
    38521 tgacagtaga tagcatgtcg atttttatag tctatagctt ggtaccgacg atgattttaa
    38581 attttttatg gattactgtt tttcaattta tttttgaaaa atattatcta taagaacgac
    38641 atataaatgt aacaaaggcg taatatttat taggcctttt tttggtatac tagtattgtc
    38701 tttaaaagaa ggagtatcta cgtaatatga agaaaaaaat cttagcgtca cttttattaa
    38761 gtacagtaat ggtttctcaa gtagctgttt taacaactgc gcatgcagaa acgactgatg
    38821 acaaaattgc tgctcaagat aataaaatta gtaacttaac agcacaacaa caagaagccc
    38881 aaaaacaagt tgaccaaatt caggagcaag tatcagctat tcaagctgag cagtctaact
    38941 tgcaagctga aaatgataga ttacaagcag aatctaagaa actcgagggt gagattacag
    39001 aactttctaa aaacattgtt tctcgtaacc aatcgttgga aaaacaagct cgtagtgctc
    39061 aaacaaatgg agccgtaact agctatatca ataccattgt aaactcaaaa tcaattacag
    39121 aagctatttc acgtgttgct gcaatgagtg aaatcgtatc tgcaaacaac aaaatgttag
    39181 aacaacaaaa ggcagataaa aaagctattt ctgaaaaaca agtagcaaat aatgatgcta
    39241 tcaatactgt aattgctaat caacaaaaat tggctgatga tgctcaagca ttgactacga
    39301 aacaggcaga actaaaagct gctgaattaa gtcttgctgc tgagaaagcg acagctgaag
    39361 gggaaaaagc aagtctatta gagcaaaaag cagcagctga ggcagaggct cgtgcagctg
    39421 cggtagcaga agcagcttat aaagaaaaac gagctagcca acaacaatca gtacttgctt
    39481 cagcaaacac taacttaaca gctcaagtgc aagcagtatc tgaatctgca gcagcacctg
    39541 tccgtgcaaa agttcgtcca acatacagta caaacgcttc aagttatcca attggagaat
    39601 gtacatgggg agtaaaaaca ttggcacctt gggctggaga ctactggggt aatggagcac
    39661 agtgggctac aagtgcagca gcagcaggtt tccgtacagg ttcaacacct caagttggag
    39721 caattgcatg ttggaatgat ggtggatatg gtcacgtagc ggttgttaca gctgttgaat
    39781 caacaacacg tatccaagta tcagaatcaa attatgcagg taatcgtaca attggaaatc
    39841 accgtggatg gttcaatcca acaacaactt ctgaaggttt tgttacatat atttatgcag
    39901 attaatttac agagggactc gaatagagcc ctcttttcag gttttaccgt gacaatccct
    39961 attaaaaatt atatcaaaat agcttgaaaa tattggaaaa gtatggtaga atgaaaattg
    40021 tcgtgtgaac gataatactc attcttgatg aattgtgaag cagttgccct tgggtcgttt
    40081 tgcgagttga agtcaagaag aggaaaaaaa caaaaaggag aaatactcat ggcagtaatt
    40141 tcaatgaaac aacttcttga ggctggtgta cactttggtc accaaactcg tcgctggaat
    40201 cctaagatgg ctaagtacat ctttactgaa cgtaacggaa tccacgttat cgacttgcaa
    40261 caaactgtaa aatacgctga ccaagcatac gacttcatgc gtgatgcagc agctaacgat
    40321 gcagttgtat tgttcgttgg tactaagaaa caagcagctg atgcagttgc tgaagaagca
    40381 gtacgttcag gtcaatactt catcaaccac cgttggttgg gtggaactct tacaaactgg
    40441 ggaacaatcc aaaaacgtat cgctcgtttg aaagaaatta aacgtatgga agaagatgga
    40501 actttcgaag ttcttcctaa gaaagaagtt gcacttctta acaaacaacg tgcgcgtctt
    40561 gaaaaattct tgggcggtat cgaagatatg cctcgtatcc cagatgtgat gtacgtagtt
    40621 gacccacata aagagcaaat cgctgttaaa gaagctaaaa aattgggaat cccagttgta
    40681 gcgatggttg acaccaatac tgatccagat gatatcgatg taatcatccc agctaacgat
    40741 gacgctatcc gtgctgttaa attgatcaca gctaaattgg ctgacgctat tatcgaagga
    40801 cgtcaaggtg aggatgcagt agcagttgaa gcagaatttg cagcttcaga aactcaagca
    40861 gattcaattg aagaaatcgt tgaagttgta gaaggtgaca acgcttaatt tatacaaata
    40921 gtaattacct aggagggcgg ggcttagccc ggctctccta ttttcaaaaa atataggaga
    40981 attaaaatgg cagaaattac agctaaactt gtaaaagagt tgcgtgaaaa atctggtgcc
    41041 ggtgttatgg acgctaaaaa agcgcttgta gaaacagacg gtgacatcga aaaagcgatt
    41101 gaattgcttc gtgaaaaagg tatggctaag gcagctaaga aagctgaccg tgttgctgca
    41161 gaaggtttga ctggtgttta tgttaacggt aatgttgcag cagttattga agtaaacgct
    41221 gaaactgact tcgttgcaaa aaacgctcaa ttcgttgaat tggtaaatac tacagctaaa
    41281 gtcattgctg aaggaaaacc tgctaataat gaagaagctc ttgctttgat aatgccttca
    41341 ggtgaaactc ttgaagctgc atacgtatct gcaacagcaa ctatcggaga gaaaatctca
    41401 ttccgtcgct ttgcattgat tgaaaaaaca gacgcacaac actttggagc ataccaacat
    41461 aacggtggac gtatcggtgt tatttcagtt gttgaaggtg gagacgaagc acttgctaaa
    41521 caattgtcaa tgcacatcgc agcgatgaaa ccaacagttc tttcttacaa agaattggat
    41581 gagcaattcg ttaaagatga gttggcacaa ttgaatcacg ttatcgacca agacaacgaa
    41641 agccgtgcaa tggttaataa accagctctt ccacacttga agtatggatc aaaagctcaa
    41701 ttaactgatg atgttattgc tcaagctgaa gctgacatca aagctgaatt ggctgcagaa
    41761 ggcaaaccag aaaaaatctg ggacaaaatt atcccaggta aaatggatcg cttcatgctt
    41821 gataatacta aagttgacca agcttacaca cttcttgcac aagtttacat catggatgac
    41881 agcaagacag ttgaagcata ccttgaatca gttaacgctt cggtagttga gtttgctcgc
    41941 tttgaagttg gtgaaggtat cgagaaagct gcaaacgact tcgaagctga agttgcagct
    42001 acaatggcag cagccttgaa taactaataa tagaaaggaa gcaatttgct tccttttttg
    42061 tatagggctt atatttttcc tgaaattgtt tttcccccag tctcactcga ttagaataat
    42121 taaaacaaaa agccctataa taagggctaa gtgcatttag gagactatac ttcaaattca
    42181 tagtaagcgc gtcataacaa ggtatctatc ctaattcccc aacttccagt atactagata
    42241 aaaaataatt atcggaggaa aggtattgta ctatgatgat tcaactaagt gatttaggtc
    42301 aagttcacat tgtttgtggc aagacagata tgaggcaggg aatagactca ttagcctatg
    42361 tagttaaaac ccactttgaa ttggattctt tctccggtca agtctttctc ttttgtggtg
    42421 gacgtaaaga ccgctttaaa gtcctttact gggatggtca aggattttgg ctactatata
    42481 aacgctttga gaacggaaaa ctgacttggc taagtacaga aaaggatgtc aaagctctcg
    42541 cacctgaaca agtagactag cttatgaagg gcttttctat cactccaaaa atatagtaga
    42601 ttgaaactag aatagtacac ctctgcttct aaaacattgt tagaaatcga tttgactgtc
    42661 ctgatcgatt tgtcctgttc ttatttcatt ttactataaa tccatcagaa agtcgtgatt
    42721 tctattgaaa tgaggacttt ctttttacac tcatctgctt tcaaaaagca ttctagtccg
    42781 tctccgatta acgatggact ttatcacctc cttctctagt ccttgtatga catcttccaa
    42841 agttcgaaag gctttattct taaatccacg tttacgaatc tctttccaca cttgttcaat
    42901 gggttcatct ctggtgtgta tggaggaata aaggtaaaac caatattagt cggaatcttt
    42961 aaggtacttg atttatgcca tatagcattg tccataacga gtaaaaaata atcatctgga
    43021 tgctcctatt cctaaagccc ctttagcgca tagctttggc tcagcttcta ttatcgctca
    43081 catcatccat cagaagttta atctgaaggt acccaattat cgccaagaag aagattgggc
    43141 taggatgggt ttaccaatca cacgtaagga aatctctaat cggcatatca agacgagtca
    43201 atactatttg gagccccttt ataacctctt gcgagagaga ctattgactc agcccttact
    43261 tcatgcggat gaaacttctt atagggtgct agagagtgat agtcagctga cttactattg
    43321 gacttttttg tctgggaaag ctgagaaaca agggattacg ctttaccatc atgtcctaat
    43381 cgatttgttc atatcttatt tcaatccatt ataaatagtg agaaatatct atcctatctt
    43441 ctagaatgtc ttccaaacgt ggaaactctc gtaaacaaag aggttttaga ggcctattta
    43501 ccatggacta aagttgtaca agaaaagtgc aaataagaaa tctccagatt aggaactatc
    43561 cgtgagttct ctagtctgga gatttttcaa tagacttcgt tattggacgg ttacaattca
    43621 taaagtgctg tagagagata acgttcaccg ttatctggtg ctagggcaag gactttttta
    43681 cctgtaccta attttttggc aacctcgatg gctccgtaga tagctgcagc tgaggaaatc
    43741 cctacaagga agccttcttt tccaccaatt tcacgtccga gtgcaagagc gtcatctgat
    43801 gttacacgaa cgataccatc ataggcttta gtatcaagtg tatcaggaat aaatccagct
    43861 gagatacctt gaattttgtg aggaccaggt ttttcaccag atagaatagc agattcatct
    43921 gcttctactg caaaaacttg aatgttagaa ttttctgatt tgagtgcatg agaaacacca
    43981 gaaatcgttc caccagtacc tactccagca acaaaggcat ctaatccatc tttaccgaaa
    44041 gcagctagta tctcagctcc tgttgttctt tcgtgtactt ctggattagc tggattgtca
    44101 aattgaagag gaaggaaacc atcacgttca gcagcgattt cttgagcctt agcaatagca
    44161 cctttcattc cctcgctacc aggagttagg acgagttcag caccataagc ttggataatt
    44221 ttacgtcgtt ctacactcat agtttcaggc ataacgatga cgactttata ccctttagca
    44281 gcacctaccc atgaaagtcc aataccggtg tttccacttg ttgcttcaac aatagtagaa
    44341 ccaagtttca gaataccatc ttgttcagct ttttcaatca tgctaagggc aatacggtct
    44401 tttacagatg aaccaggatt aaatgcttca agctttatat agacgtctgc agcaccttct
    44461 ggcacgatgt tgttaagttt aacaatcggt gtttgaccga ttaattcagt aatgttgtta
    44521 taaatagaca tatgaatacc tctttgtata agatatcttt agtataacgc tatccatacc
    44581 atttgtaaaa tatagaaatc ctatcgaagc gataggattt ctatatatca tagatcaaag
    44641 tcattaattt tcaagtgatt gcgttcagtt aattctagta aataggtgtg aaggaaaacg
    44701 atatcaggtt tcgtttgaat tgcttcattt tctagtatat tttggtacgc atctttttat
    44761 tttgctcgct aaagtagttg tgagaatgtc aacgaaacgc tgtattattc attgtttaca
    44821 ctaagtttac aggaacttca acctctcgta aaccttggtc agttaaagtg acttttccat
    44881 taaaaaactc cacaagtgca gctttaatag tttctttttc ttctttatca acataaatca
    44941 tcgtatcgac ttgatctgta aagtttgtat ccagctccat gagatcatgt tctttaagga
    45001 agttactgta ctcttggtac tgagcataag acatttgaat agcaatgcca gcctgttctt
    45061 ttatttcaat aataccaatt tctttgacag ctaaggcgac actgccggcg taagcacgaa
    45121 ttagtcctcc agcgcctagt ttaataccac caaagtagcg tgtcacgacc acacagacat
    45181 tggtgagatt gtgattttct agtaccccaa gcatgggaac accagcagta ccactaggct
    45241 caccatcatc acttgtacgt ttaatttcac tacgttctcc aataataaag gcagagcagt
    45301 tatgtgtcgc tttgtagtgt tcttttttga tggcagtaat gaagtcacga gcctcttctt
    45361 cgctataaac acgcttggca tggcagataa agcgagattt tttgatttct tcttggactt
    45421 ggccgtcctc tttaattgtc ctaaattcca tgatttaatt gtactaaaaa atcatctaaa
    45481 gtgctagttt ttacgaataa agaagtatga aagtaaattt agattatctc ggtcgtttat
    45541 ttactgagaa tgaattaaca gaagaagaac gtcagttggc ggagaaactt ccagcaatga
    45601 gaaaggagaa ggggaaactt ttctgtcaac gttgtaatag tactattcta gaagaatggt
    45661 atttgcccat cggtgcttac tattgtcgag agtgcttgct gatgaagcga gtcagaagtg
    45721 atcaaacttt atactatttt ccgcaggagg attttccgaa gcaagatgtt ctcaaatggc
    45781 gcggccaatt aactcctttt caagagaagg tgtcagaggg actgcttcaa gcagtagaca
    45841 agcaaaagcc aaccttagtt catgcggtaa caggagctgg aaagacagaa atgatttatc
    45901 aagtagtggc taaagtgatc aatgcgggtg gtgcagtgtg tttggctagt cctcgcatag
    45961 atgtttgttt ggagctgtac aagcgcctgc aacaggattt ttcttgcgga atagctttgc
    46021 tacatggaga atcggaacct tattttcgaa caccactagt tgttgcaaca acccatcagt
    46081 tattgaagtt ttatcaagct tttgatttgc tgatagtgga tgaagtagat gcttttcctt
    46141 atgttgataa tcccatgctt taccacgctg tcaagaatag tgtaaaggag aatggattga
    46201 gaatcttttt aacagcgact tcgaccaatg agttagataa aaaggtccgt ttaggagaac
    46261 taaaaagact gaatttaccg agacggtttc atggaaatcc gttgattatt ccaaaaccaa
    46321 tttggttatc ggattttaat cgctacttag acaagaatcg tttgtcacca aagttaaagt
    46381 cctatattga gaagcagaga aagacagctt atccgttact catttttgct tcagaaatta
    46441 agaaagggga gcagttagca gaaatcttac aggagcaatt tccaaatgag aaaattggct
    46501 ttgtatcttc tgtaacagag gatcgattag agcaagtaca agcttttcga gatggagaac
    46561 tgacaatact tatcagtacg acaatcttgg agcgtggagt taccttccct tgtgtggatg
    46621 ttttcgtagt agaggccaat catcgtttgt ttaccaagtc tagtttgatt cagattggtg
    46681 gacgagttgg acgaagcatg gatagaccga caggagattt gcttttcttc catgatgggt
    46741 taaatgcttc aatcaagaag gcgattaagg aaattcagat gatgaataag gaggctggtc
    46801 tatgaagtgc ttgttatgtg ggcagactat gaagactgtt ttaactttta gtagtctctt
    46861 acttctgagg aatgatgact cttgtctttg ttcagactgt gattctactt ttgaaagaat
    46921 tggggaagag aactgtccaa attgtatgaa aacagagttg tcaacaaagt gtcaagattg
    46981 tcaactttgg tgtaaagaag gagttggagt cagtcataga gcgattttta cttacaatca
    47041 agctatgaag gattttttca gtcggtataa gtttgatgga gacttcctgt taagaaaagt
    47101 tttcgcttca tttttaagtg aggagttgaa aaagtacaaa gagtatcaat ttgttgtaat
    47161 tcccctaagt cctgatagat atgctaatag aggatttaat caggttgagg gcttggtaga
    47221 ggcagcaggc tttgagtatc tagatttatt agagaaaaga gaagagagag ccagttcttc
    47281 taaaaatcgt tcagagcgct tggggacaga acttcctttc tttattaaaa gtggagtcac
    47341 tattcctaaa aaaatcctac ttatagatga tatctatact acaggagcaa ctataaatcg
    47401 tgttaagaaa ctgttggaag aagccggtgc taaggatgta aaaacatttt cccttgtaag
    47461 atgaggaaaa aatttgcaaa aatagaatga aagcgttata ataaaatcat aaaaacaaaa
    47521 atgtttagaa agaaggtact catatgatta aatatagtat ccgtggtgaa aacctagaag
    47581 taacagaagc aattcgtgat tatgtagttt ctaaactcga aaagatcgaa aagtacttcc
    47641 aaccagaaca agagttggat gcccgaatta acttaaaagt ttatcgtgaa aaaacggcta
    47701 aagtggaagt aacgattccg cttggatcta ttactctccg cgcagaagat gtatctcaag
    47761 atatgtatgg ttcaattgac cttgtaactg ataaaattga acgtcagatt cgtaaaaata
    47821 aaacaaaaat cgagcgtaaa aataaaaata aggtagcaac tggtcaatta tttacagatg
    47881 ctttggtgga agattcaaat attgtccagt ctaaagttgt tcgttcaaaa caaattgatt
    47941 taaaaccaat ggatttggaa gaagcaattc tacaaatgga tttattgggg catgatttct
    48001 ttatctatgt ggatgttgaa gatcagacaa ccaatgtgat ttatcgtcgt gaggatggcg
    48061 aaattggttt gttagaggtt aaagaatctt aatttcaata tgtgtaaagg taggtttact
    48121 gaattgtaaa ctaccttttt cttataattg atagaattac tgattacact tttaaaaagt
    48181 cgctttttga ggggtattat ggtataatgg gtgccaagag gttttgaatg aaaaaatttt
    48241 atgtaagtcc aatttttcct attctagtag gattgattgc gtttggagtc ttatccactt
    48301 tcattatttt tgttaataat aatctgttga cggttttaat tttgtttctt tttgtaggag
    48361 gctatgtttt tttatttaag aaactgagag tgcattatac aaggagtgat gtagaacaga
    48421 tacagtatgt aaaccaccaa gcggaagaaa gtttgacagc tctattggaa cagatgcctg
    48481 taggtgttat gaaattgaat ttatcttctg gagaggttga gtggtttaat ccctatgctg
    48541 aattgatttt gaccaaggaa gatggtgatt ttgatttaga agctgttcaa acgattatca
    48601 aggcttcagt aggaaatccg tctacttatg ccaagcttgg tgagaagcgt tatgctgttc
    48661 atatggatgc ttcttccggt gttttgtatt ttgtagatgt atccagggaa caagccataa
    48721 cagatgaatt ggtaacaagt cgaccagtga ttgggattgt ctctgtggat aattatgatg
    48781 atttggagga tgcaacttct gagtcagata ttagtcaaat caatagtttt gtagctaatt
    48841 ttatatcaga gttttcagaa aaatacatga tgttttctcg tcgggtaagt atggatcgat
    48901 tttatctatt tactgactac acggtgcttg agggcttgat gaatgataaa ttttctgtta
    48961 ttgatgcttt cagagaagag tcgaaacaga gacagttgcc cttgacttta agtatgggat
    49021 tttcttatgg cgatggaaat catgatgaga tagggaaagt tgctttgctc aatttgaact
    49081 tggctgaagt acgtggtggc gaccaggtgg ttgttaagga aaacgacgaa acgaaaaatc
    49141 cagtttattt tggtggtggg tctgctgctt caatcaagcg tacacggact cgtacgcgcg
    49201 ctatgatgac agctatttca gataagattc ggagtgtaga tcaggttttt gtagtcggtc
    49261 acaaaaattt agacatggat gctttgggct ctgctgtagg tatgcagttg ttcgccagca
    49321 atgtgattga aaatagctat gctctttatg atgaagaaca aatgtctcca gatattgaac
    49381 gagctgtttc attcatagaa aaagaaggag ttacgaagtt gttgtctgtt aaggatgcaa
    49441 tggggatggt gaccaatcgt tctttgttga ttcttgtaga ccattcaaag acagccttaa
    49501 cattatcaaa agaattttat gatttattta cccaaaccat tgttattgac caccatagaa
    49561 gggatcagga ttttccagat aatgcagtca ttacttatat cgaaagtggt gcaagtagtg
    49621 ccagtgagtt ggtaacggaa ttgattcagt tccagaattc taagaaaaat cgtttgagtc
    49681 gtatgcaagc aagtgttttg atggctggta tgatgttgga tactaaaaat ttcacctcgc
    49741 gagtaactag tcggacattt gatgttgcta gctatctcag aacgcgcgga agtgatagta
    49801 ttgctatcca ggaaattgct gcgacagatt ttgaagaata tcgtgaggtt aatgaactta
    49861 ttttacaggg gtgtaaatta ggttcagatg tactaatagc agaggctaag gacacgaaat
    49921 gctatgatac agttgttatt agtaaggcag cagatgccat gttagccatg tcaggtattg
    49981 aagcgagttt tgttcttgcg aagaatacac aaggatttat ctctatctca gctcgaagtc
    50041 gtagtaaact gaatgtacaa cggattatgg aagagttagg cggtggaggc cactttaatt
    50101 tggcagcagc tcaaattaaa gatgtaacct tgtcagaagc aggtgaaaaa ctgacagaaa
    50161 ttgtattaaa tgaaataaag gaaaaggaga aagaagaatg aaagtaatct ttttagcaga
    50221 tgttaaagga aaaggtaaaa aaggcgaaat taaggaagta ccaacagggt atgcgcaaaa
    50281 ctttcttatc aaaaagaatc tagccaaaga agcgactgct caagctgtag gtgaacttcg
    50341 tggtaaacaa aaatcggaag aaaaagctca cgctgagatg attgcagaag gaaaagcaat
    50401 taaagcacaa cttgaagcag aagaaactgt tgtagaattt gttgaaaaag ttggtccaga
    50461 tggtcgtacc tttggttcta ttaccaataa gaagattgca gaagaattgc aaaagcaatt
    50521 tggaattaag attgataaac gtcatattca agtacaagct ccgattcgag cggttggttt
    50581 gattgatgtg ccagtgaaaa tctatcaaga tatcacaagt gtaatcaatc ttcgtgtgaa
    50641 agaaggataa gtttacacct tcttgacaag attgtaaaag gaagggaagt ctgatggcag
    50701 aagtagaaga gttacgagta caacctcaag atatcttagc tgagcaatcc gttttagggg
    50761 ctatctttat tgatgagagt aaacttgttt ttgtgcgaga atacattgag tctcgggact
    50821 tttttaagta tgcccatcgt ttgattttcc aagccatggt cgatttatcc gatcgtggtg
    50881 atgccataga tgcaacaacg gttcgtacta tccttgataa tcaaggtgat ttacagaata
    50941 ttggtggctt gtcttacttg gttgagattg ttaattctgt gccaacttct gctaatgcgg
    51001 agtattatgc taagattgtt gcagaaaaag caatgctacg tcgtttaatt gccaagttga
    51061 cagagtctgt caaccaagct tacgaagcgt cacaaccagc tgatgaaatt attgctcagg
    51121 cagaaaaagg gttgattgat gtcagtgaaa atgcaaatcg aagcgggttt aagaacattc
    51181 gagatgtgtt gaatctcaac tttggaaatc tggaagctcg ctcgcaacaa acgaccgata
    51241 ttacaggtat tgcgacaggt tatcgtgatt tggatcatat gacaacagga cttcatgagg
    51301 aggagttgat tatcttagca gctcgtccag cagttggtaa gacagcattt gccttgaata
    51361 tcgctcagaa tattgggact aagttggaca aaacggttgc tattttttca ctcgaaatgg
    51421 gtgcggaaag cttggtagac cgtatgttag ctgcagaagg cttggtggag tcacattcta
    51481 tccgtacagg gcaattgaca gatgaggagt ggcaaaaata tactattgct cagggtaatc
    51541 tagctaacgc cagtatctat atcgatgata cgccaggtat tcggattaca gagattcgtt
    51601 ctcgttctcg taaattggct caagaaactg gaaatcttgg tttgattttg atagactatt
    51661 tgcaacttat cacgggaact ggtcgagaaa atcgtcaaca agaagtttct gaaatttctc
    51721 gtcagttgaa aatactagcc aaggaattga aggttccagt aatcgctctg agtcagcttt
    51781 ctcgtggtgt agaacaacgt caggacaaga gaccggtctt gtctgatatt cgtgaatctg
    51841 ggtctattga gcaggacgct gatatcgtag cttttctcta tcgcgatgac tactatgaac
    51901 gtggtggtga agaagaggag ggtatcccaa ataataaggt ggaagttatt atcgagaaaa
    51961 accgtagtgg agctcgtgga acagtggaat tgattttcca aaaagaatac aataaatttt
    52021 caagtatctc aaagagggag gcataagatg tcagatgcat ttacagatgt agccaagatg
    52081 aaaaaaatca aagaagaaat caaggcacat gagggacaag tcgtagaaat gactttggag
    52141 aatggtcgta agcgccaaaa aaatagattg ggtaagctaa ttgaagttta tccatcccta
    52201 tttattgtgg agtttgggga tgtggaagga gataaacaag ttaatgttta cgttgaatcc
    52261 tttacttact cagatattct tacagaaaag aatttgattc attatcttga ctaaagtgag
    52321 aaattttctc actttttctt ttttctccga ataatttagg tgaaggcaat catcgcttta
    52381 tattattttt taaggaggaa gaatgaaaat tttaccgttt atagcaagag gaacaagtta
    52441 ttacttgaag atgtcagtta aaaagcttgt tcctttttta gtagtaggat tgatgctagc
    52501 agctggtgat agtgtctatg cctattccag aggaaatgga tcgattgcgc gtggggatga
    52561 ttatcctgct tattataaaa atgggagcca ggagattgat cagtggcgca tgtattctcg
    52621 tcagtgtact tcttttgtag cctttcgttt gagtaatgtc aatggttttg aaattccggc
    52681 agcttatgga aatgcgaatg aatggggaca tcgtgctcgt cgggaaggtt atcgtgtaga
    52741 taatacaccg acgattggtt ccattacttg gtctactgca ggaacttatg gtcatgttgc
    52801 ctgggtgtca aatgtaatgg gagatcagat tgagattgag gaatataact atggttatac
    52861 agaatcctat aataaacgag ttataaaagc aaacacgatg acaggattta ttcattttaa
    52921 agatttggat agtggcagtg ttgggaatag tcaatcctca gcttcaacag gcggaactca
    52981 ttattttaag accaagtctg ctattaaaac tgaaccccta gttagtgcaa ctgtgattga
    53041 ttactattat cctggggaga aggttcatta tgatcagata cttgaaaaag acggctataa
    53101 gtggttgagt tatactgcct ataatggaag ctatcgttat gttcaattgg aggctgtgaa
    53161 taaaaatcct ctaggtaatt ctgttctttc ttcaacagga ggaactcatt attttaagat
    53221 caagtctgct attaaaactg aacccctagt tagtgcaact gtgattgatt actattatcc
    53281 tggagagaag gttcattatg atcagatact tgaaaaagac ggctataagt ggttgagtta
    53341 tacggcttat aacggaagtc gtcgctatat acagctagag ggagtgactt cttcacaaaa
    53401 ttatcagaat caatcaggaa atatctctag ctatggatcc aataatagtt caactgtcgg
    53461 ttggaagaaa ataaatggta gttggtatca tttcaaatca aatggttcta aatcaacagg
    53521 atggctgaaa gacggttcta gctggtatta tttgaaatta tctggtgaaa tgcagacagg
    53581 atggttaaag gagaatggct cgtggtatta tctgggtagt tcaggggcaa tgaaaacagg
    53641 ctggtaccag gtctctggta agtggtatta ttcttactct tcaggcgcct tagctattaa
    53701 tacgacggtg gatggctaca gagtaaacag tgatggagaa cgagtataga aaattggagt
    53761 aggagaaatt tcctgctctt ttctgtaagc agtttccttg acatttttct tattttgcgc
    53821 tatcatatat acatataata tatgggagtc tgtgtacagt ctgagaggaa gtgttaaact
    53881 tcgaccgcac ctgatctggg taatgccagc ggagggaacg atacttagtc taattttgca
    53941 ccttttccat gtatggtaaa ggtttttctt ttttttaaaa ggaaaacgag aagaggaggt
    54001 tcttatgaaa gcaagcattg ccttgcaagt tttaccccta gcacagggga ttgatcggat
    54061 agctgttatt gatcaggtca ttgcttatct gcaaactcaa gaagtgacga tggtagtgac
    54121 accatttgaa acggtcttgg aaggggagtt tgatgagctt atgcgcattc taaaagaagc
    54181 gctggaagtg gcagtgcagg aggcagacaa tgtctttgcc aatgtcaaaa taaatgtagg
    54241 agagatttta agtattgatg agaaacttga aaagtatact gagacgacac attagtctat
    54301 tgggctttct cggagtattg tcaatctggc agttagcagg ttttcttaaa cttctcccca
    54361 agtttatcct gccgacacct cttgaaattc tccagccctt tgttcgtgac agagaatttc
    54421 tctggcacca tagctgggcg accttgagag tggctttact ggggctgatt ttgggagttt
    54481 tgattgcctg tcttatggct gtgctcatgg atagtttgac ttggctcaat gacctgattt
    54541 accctatgat ggtggtcatt cagaccattc cgaccattgc catagctcct atcctggtct
    54601 tgtggctggg ttatgggatt ttgcccaaga ttgtcttgat tatcttaaca acaacctttc
    54661 ccatcatcgt tagtattttg gacggtttta ggcattgtga caaggatatg ctgactttgt
    54721 ttagtctgat gcgagcaaac ccttggcaaa tcctgtggca ttttaaaatc ccggttagcc
    54781 tgccttactt ttatgcaggt ctgagggtca gtgtctccta cgcctttatc acaactgtgg
    54841 tatctgagtg gttgggaggt tttgaaggtc ttggtgttta tatgattcag tctaaaaaac
    54901 tgtttcagta tgataccatg tttgccatta ttattctggt gtcgattatc agtcttttgg
    54961 gtatgaagct ggtcgatatc agtgaaaaat atgtgattaa atggaaacgt acttaaaata
    55021 gagcgttttt gaaaaagaaa agaggaaatc aaaatgaaga aaacatggaa agtgttttta
    55081 acgcttgtaa cagctcttgt agctgttgtg cttgtggcct gtggtcaagg aactgcttct
    55141 aaagacaaca aagaggcaga acttaagaag gttgacttta tcctagactg gacaccaaat
    55201 accaaccaca cagggcttta tgttgccaag gaaaaaggtt atttcaaaga agctggagtg
    55261 gatgttgatt tgaaattgcc accagaagaa agttcttctg acttggttat caacggaaag
    55321 gcaccatttg cagtgtattt ccaagactac atggctaaga aattggaaaa aggagcagga
    55381 atcactgccg ttgcagctat tgttgaacac aatacatcag gaatcatctc tcgtaaatct
    55441 gataatgtaa gcagtccaaa agacttggtt ggtaagaaat atgggacatg gaatgaccca
    55501 actgaacttg ctatgttgaa aaccttggta gaatctcaag gtggagactt tgagaaggtt
    55561 gaaaaagtac caaataacga ctcaaactca atcacaccga ttgccaatgg cgtctttgat
    55621 actgcttgga tttactacgg ttgggatggt atccttgcta aatctcaagg tgtagatgct
    55681 aacttcatgt acttgaaaga ctatgtcaag gagtttgact actattcacc agttatcatc
    55741 gcaaacaacg actatctgaa agataacaaa gaagaagctc gcaaagtcat ccaagccatc
    55801 aaaaaaggct accaatatgc catggaacat ccagaagaag ctgcagatat tctcatcaag
    55861 aatgcacctg aactcaagga aaaacgtgac tttgtcatcg aatctcaaaa atacttgtca
    55921 aaagaatacg caagcgacaa ggaaaaatgg ggtcaatttg acgcagctcg ctggaatgct
    55981 ttctataaat gggataaaga aaatggtatc cttaaagaag acttgacaga caaaggcttc
    56041 accaacgaat ttgtgaaata atgacagaaa ttagactaga gcacgtcagt tatgcctatg
    56101 gtcaggagag gattttagag gatatcaacc tacaggtgac ttcaggcgaa gtggtttcca
    56161 tcctaggccc aagtggtgtt ggaaagacca ccctctttaa tctaatcgct gggattttag
    56221 aagttcagtc agggagaatt gtccttgatg gtgaagaaaa tcccaagggg cgcgtgagtt
    56281 atatgttgca aaaggatctg ctcttggagc acaagacggt gcttggaaat atcattctgc
    56341 ccctcttgat tcaaaaggtg gataaggcag aagctatttc ccgagcggat aaaattcttg
    56401 cgaccttcca gctgacagct gtaagagaca agtatcctca tgaacttagc ggtgggatgc
    56461 gccagcgtgt agccttactc cggacctacc tttttgggca caagctcttt ctcttagatg
    56521 aggcctttag cgccttggat gagatgacaa agatggaact ccacgcttgg tatcttgaga
    56581 ttcacaagca gttgcagcta acaaccctga tcatcacgca tagtattgag gaggccctca
    56641 atctcagcga ccgcatctat atcttgaaaa atcgccctgg gcagattgtt tcagaaatta
    56701 aactagattg gtctgaagat gaggacaagg aagtccaaaa gattgcctac aaacgtcaaa
    56761 tcttggcaga attaggctta gataagtaga aaaataggga gttggtgaag attatccttt
    56821 accagcgccc tttttctttt aaaaatgaga aaatttcggt ataatagtca aacaaggtca
    56881 aggtttaaag agagaggtgg gtttgttatg agatttaaaa atacatcgga tcatattgag
    56941 gcctacatca aggcgatttt agatcaatct ggtatcgtgg agttgcaacg gagtcagttg
    57001 gcagatacct ttcaggttgt tcctagtcag attaactacg tgatcaagac acgctttacg
    57061 gaaagtagag gctacttggt tgaaagtaag cgtggtggtg gaggctacat tcgtatagga
    57121 cgaattgagt tttctagtca tcatgaaatg ctccgggagc tgctttactc gattggtgag
    57181 cgagtcagtc aagaaattta tgaggatatt ctccagcttt tggttgagca ggaattgatg
    57241 accaagcagg agatgaattt gctagaatca gtagctttgg atcgcgtttt aggagaagaa
    57301 gctccagttg ttcgagcaaa catgctacgt cagatcatac aagaggtaga tagaaaaggg
    57361 aagtaagatg aactattcaa aagcattgaa tgaatgtatc gaaagtgcct acatggttgc
    57421 tggacatttt ggagctcgtt atctagagtc ttggcacttg ttgattgcca tgtctaatca
    57481 cagttatagt gtagcagggg caactttaaa tgattatccg tatgagctgg accgtttaga
    57541 agaggtggct ttggaactga ctgaaacgga ctatagccag gatgaaactt ttacggaatt
    57601 gccgttctcc cgtcgtttgc agattctttt tgacgaagca gagtatgtag cgtcagtggt
    57661 ccatgctaag gtgctaggga cagagcacgt cctctatgcg attttgcatg atggcaatgc
    57721 cttggcgact cgtatcttgg agagggctgg tttttcttat gaagacaaga aagatcaggt
    57781 caagattgct gctcttcgtc gaaatttaga agaacgggca ggctggactc gtgaagatct
    57841 caaggcttta cgccaacgcc atcgtacagt agctgacaag caaaattcta tggccaatat
    57901 gatgggcatg ccgcagactc ctagtggtgg tctcgaggac tatacgcatg atttgacaga
    57961 gcaagcgtgt tctggcaagt tagaaccagt catcggtcgg gacaaggaaa tctcacgtat
    58021 gattcaaatc ttgagccgga agactaagaa caaccctgtc ttggttgggg atgctggtgt
    58081 cgggaaaaca gctctggcgc ttggtcttgc tcagcgtatt gctagtggtg acgtgcctgc
    58141 ggaaatggct aagatgcgcg tgttagaact tgatttgatg aatgtcgttg cagggacacg
    58201 cttccgtggt gactttgaag aacgcatgaa taatatcatc aaggatattg aagaagatgg
    58261 ccaagtcatc ctctttatcg atgaactcca caccatcatg ggttctggta gcgggattga
    58321 ttcgactctg gatgcggcca atatcttgaa accagccttg gcgcgtggaa ctttgagaac
    58381 ggttggtgcc accactcagg aagaatatca aaaacatatc gaaaaagatg cggctctttc
    58441 tcgtcgtttc gctaaagtga cgattgaaga accaagtgtg gcagatagta tgactatttt
    58501 acaaggtttg aaggcgactt atgagaaaca tcaccgtgta caaatcacag atgaagcggt
    58561 tgaaacagcg gttaagatgg ctcatcgtta tttaaccagt cgtcacttgc cagactctgc
    58621 tatcgacctc ttggacgaag cagcagcaac agtgcaaaat aaggtaaagt atgtaaaagc
    58681 agacgattca gatttgagtc cagctgataa ggccctgatg gatggcaagt ggaaacaggc
    58741 agcccagcta atcgcaaaag aagaggaagt acctgtctac aaagacttgg tgacagagtc
    58801 tgatattttg accaccttga gtcgcttgtc aggaatccca gttcaaaaac tgactcaaac
    58861 ggatgctaag aagtatctaa atcttgaagc agaactccat aaacgtgtca tcggtcaaga
    58921 tcaagctgtt tcaagcatta gtcgtgcgat tcgccgtaat cagtcaggaa ttcgcagtca
    58981 caagcgtccg atcggttcct ttatgttcct aggacccaca ggtgtcggta agaccgagtt
    59041 ggccaaggct ttggcagaag ttctctttga tgacgaatca gcccttatcc gctttgatat
    59101 gagtgagtat atggagaaat tcgcagccag ccgtctaaat ggagctcctc caggctatgt
    59161 gggttacgaa gaaggtggag agttgacaga gaaggttcgc aataaaccct attccgttct
    59221 cctctttgat gaggtagaga aggcccaccc agatatcttt aatgttctct tgcaagttct
    59281 ggatgacggt gttttgacag atagcaaggg acgcaaggtc gatttttcaa ataccattat
    59341 cattatgaca tcgaacctag gtgcgactgc ccttcgtgat gataagactg ttggttttgg
    59401 ggctaaggat attcgttttg accaggaaaa tatggaaaaa cgcatgtttg aagaactgaa
    59461 aaaagcttat agaccggaat tcatcaaccg tattgatgag aaggtggtct tccatagcct
    59521 atctagtgac catatgcagg aagtggtgaa gattatggtc aagcctttag tggcaagttt
    59581 ggctgaaaaa ggcattgact tgaaattaca agcttcagct ctgaaattgt tagcaaatca
    59641 aggatatgac ccagagatgg gagctcgccc acttcgcaga accctgcaaa cagaagtgga
    59701 ggacaagttg gcagaacttc ttcttaaggg agatttagtg gcaggcagca cacttaagat
    59761 tggtgtcaaa gcaggccagt taaaatttga tattgcataa aagaataaaa gtatcagcat
    59821 ctgaccataa gtcacagtgg agtgaaactc aatgaaaatc aaagagcaaa ctaggcagct
    59881 agccgcaggt tgctcaaaac actggtttga ggttgcagat agagctgacg tggtttgaag
    59941 agattttcga agagtatgaa actaaaacct atagcttcta aacgatccgt ggttttcacc
    60001 attcaacaca aaattcatat gtttattacc ctccgtcgta tttgtcttag agcgtgtgta
    60061 gtagaaaaag agcagtctta tctgaaattt ttattctttc aaaagagacc tgtttctttt
    60121 ttgcatgtca aatccgttct agctggtatt tgaaaaatca aactaatatt caatgaaaat
    60181 caaagaacaa actaggcagc tagccacagg ttgctcaaaa cactgttttt agattgtaga
    60241 tagagctgac gtggtttgaa gagattttcg aagagtataa gctgcaagat gaatgatttt
    60301 cttgtattga cgttgttgtt gacaaaaagt agcggataaa tgaaatccat tccattatca
    60361 tagatgatag gctggtagga aattttcaaa tagcatacag gaaatagatg tatggagttc
    60421 tggtagtaga aagggagaga gatgaacatt ttagttgcag atgacgagga aatgattaga
    60481 gaaggaattg cagcatttct gacagaagag ggttatcatg tcattatggc taaggatgga
    60541 caagaggtct tggaaaaatt tcaagatctc cctatccatc tcatggtact ggatttaatg
    60601 atgcctagga agagtggttt tgaagtgtta aaagaaatca atcaaaagca cgatattcct
    60661 gtcatcgtct tgagtgctct gggagatgaa actactcagt cacaggtatt tgatctctat
    60721 gctgatgatc atgtgacaaa acctttttct ttggtactgc ttgtcaagcg tattaaggcg
    60781 cttatcagac gttactacgt catagaggat atttggcgat atcaggatgt aacagtggat
    60841 tttacctctt acaaagcaca ttataaaaat gaagaaattg atctcaaacc aaaggaatta
    60901 ctggtactaa agtgtttgat tcagcataaa aatcaagttt taagtagaga gcagatattg
    60961 gaagaaattt caaaagatgt agctgattta ccttgtgata gggtcgttga tgtctatatt
    61021 cgtactcttc gcaaaaaatt agctttagat tgtatcgtga ctgtgaaaaa tgttgggtat
    61081 aagattagct tatgataaaa aatcctaaat tattaaccaa gtctttttta agaagttttg
    61141 caattctagg tggtgttggt ctagtcattc atatagctat ttatttgacc tttccttttt
    61201 attatattca actggagggg gaaaagttta atgagagcgc aagagtgttt acggagtatt
    61261 taaagactaa gacatctgat gaaattccaa gcttactcca gtcttattca aagtccttga
    61321 ccatatctgc tcaccttaaa agagatattg tagataagcg gctccctctt gtgcatgact
    61381 tggatattaa agatggaaag ctatcaaatt atatcgtgat gttagatatg tctgttagta
    61441 cagcagatgg taaacaggta acagtgcaat ttgttcacgg ggtggatatc tacaaagaag
    61501 caaagaatat tttgcttttg tatctcccat atacattttt ggttacaatt gctttttcct
    61561 ttgttttttc ttatttttat actaaacgct tgctcaatcc tcttttttac atttcagaag
    61621 tgactagtaa aatgcaagat ttggatgaca atattcgttt tgatgaaagt aggaaagatg
    61681 aagttggtga agttggaaaa cagattaatg gtatgtatga gcacttgttg aaggttattc
    61741 atgagttgga aagtcgtaat gagcaaattg taaaattgca aaatcaaaag gtttcctttg
    61801 tccgtggagc atcacatgag ttgaaaaccc ctttagccag tcttagaatt atcctagaga
    61861 atatgcagca taatattgga gattacaaag atcatccaaa atatattgca aagagtataa
    61921 ataagattga ccagatgagc cacttattag aagaagtact ggagtcttct aaattccaag
    61981 agtggacaga gtgtcgtgag accttgactg ttaagccagt tttagtagat attttatcac
    62041 gttatcaaga attagctcat tcaataggtg ttacaattga aaatcaattg acagatgcta
    62101 ccagggtcgt catgagtctt agggcattgg ataaggtttt gacaaacttg attagtaatg
    62161 caattaaata ttcagataaa aatgggcgcg taatcatatc cgagcaagat ggctatctct
    62221 ctatcaaaaa tacatgtgcg cctctaagtg accaagaact agaacattta tttgatatat
    62281 tctatcattc tcaaatcgtg acagataagg atgaaagttc cggtttgggt ctttacattg
    62341 tgaataatat tttagaaagc tatcaaatgg actatagttt tctcccttat gaacacggta
    62401 tggaatttaa gattagcttg tagacagatt agttttttat taaagttcat atagggttaa
    62461 cataagtgtg ttattctttg catagataaa agaaaggata ctaatatggt attagcgatt
    62521 attttagtaa cattctttat tcgattgatt tttttaaagc gttcgataga gaatgagaaa
    62581 cgaatcctta gcaatggcgg gaaagaattt ggagttgaga atacaaaacg attaactatg
    62641 gctcatattg ttttttatct ctcttgcttg gttgaggcag tggtgcacaa gacaattttt
    62701 gatggcatgg gtatggttgg tttggtcttg cttatttttt ctatgctgat gttgatgttg
    62761 gtgattcact tgttgggaga tatttggaca gtgaagctta tgcttgtcaa taatcacaaa
    62821 tatgtagatc atatcttgtt taggacagta aaacatccta attacttttt aaatattctt
    62881 cctgagttga ttggcttgac cttgttgagt catgcttatg tgacttttgt tttagttttt
    62941 ccagtttatg cagttatttt gtatcgacga atagctgaag aggaaaagct attacatgaa
    63001 gttataatcc caaatggaag cataaagaga taaatacaaa attcgattta tatacagttc
    63061 atattgaagt gatatagtaa ggttaaagaa aaaatataga aggaaataaa catgtttgca
    63121 tcaaaaagcg aaagaaaagt acattattca attcgtaaat ttagtattgg agtagctagt
    63181 gtagtagttg ccagtcttgt tatggggagt gtggttcatg cgacagagaa cgagggaagt
    63241 acccaagcag ccactttttc taatatggca aataaaagtc agacagaaca aggagaaatc
    63301 aatatagaac gagataaggc aaagacagcg gtcagtgaat ataaagaaaa aaaagtgagt
    63361 gagatctata caaaattaga gagagataga cataaagata ctgtagatct agttaacaag
    63421 ttgcaagaga ttaagaacga gtatttgaat aaaatagttc aatcaacctc aaaaaccgaa
    63481 atacagggac ttattacaac aagtcgatca aaactagatg aagctgtgtc taaatataaa
    63541 aaggccccat cttcttcgtc aagttcaggc tcttccacta aaccggaagc ttcagataca
    63601 gcgaagccaa acaagccgac agaactagaa aaaaaggtag cagaagctga gaagaaggtt
    63661 gaagaagcta agaagaaagc caaggatcaa aaagaagaag attaccgtaa ctacccaacc
    63721 attacttaca aaacgcttga acttgaaatt gctgagtccg atgtggaagt taaaaaagcg
    63781 gagcttgaac tagtaaaaga ggaagctaag gaacctcgaa acgaggaaaa agttaagcaa
    63841 gcaaaagcga aagttgagag tgaagaaact gaggctacaa ggttagaaaa aatcaagaca
    63901 gatcgtaaaa aagcagaaga agaagctaaa cgaaaagcag cagaagaaga taaagttaaa
    63961 gaaaaaccag ctgaacaacc aaaaccagcg ccggctcctc aaccagaaaa accagctcca
    64021 aaaccagaga atccagctcc aaaaccagag aatccagctc caaaaccaga aaaaccagct
    64081 gaacaacaag ctgaagaaga ctatgctcgt agatcagaag aagaatataa tcgcttgact
    64141 caacagcaac cgccaaaaac tgaaaaacca gcacaaccat ctactccaaa aacaggctgg
    64201 aaacaagaaa acggtatgtg gtacttctac aatactgatg gttcaatggc gacaggatgg
    64261 ctccaaaaca atggctcatg gtactacctc aacagcaatg gcgctatggc gacaggatgg
    64321 ctccaaaaca atggttcatg gtactatcta aacgctaatg gttcaatggc aacaggatgg
    64381 ctccaaaaca atggttcatg gtactaccta aacgctaatg gttcaatggc gacaggatgg
    64441 ctccaataca atgg
//
LOCUS       DAVBNO010000005        60410 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVBNO010000005 DAVBNO010000000
VERSION     DAVBNO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 60410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60410
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.005"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..580
                     /locus_tag="VQ846_000291"
     CDS             <1..580
                     /locus_tag="VQ846_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET3074941.1"
                     /translation="RLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEM
                     LKNPRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGA
                     MPELFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIG
                     SVRSPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            598..1482
                     /locus_tag="VQ846_000292"
     CDS             598..1482
                     /locus_tag="VQ846_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET3074942.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(1498..2349)
                     /locus_tag="VQ846_000293"
     CDS             complement(1498..2349)
                     /locus_tag="VQ846_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET3074943.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            2549..4606
                     /gene="pbp2b"
                     /locus_tag="VQ846_000294"
     CDS             2549..4606
                     /gene="pbp2b"
                     /locus_tag="VQ846_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET3074944.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGN
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            4617..5213
                     /gene="recR"
                     /locus_tag="VQ846_000295"
     CDS             4617..5213
                     /gene="recR"
                     /locus_tag="VQ846_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET3074945.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            5408..6451
                     /locus_tag="VQ846_000296"
     CDS             5408..6451
                     /locus_tag="VQ846_000296"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET3074946.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFAKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            6535..7908
                     /locus_tag="VQ846_000297"
     CDS             6535..7908
                     /locus_tag="VQ846_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET3074947.1"
                     /translation="MKLTIYEVAQAVGAKNDVSLFADAQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLTP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQIILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            7895..8506
                     /locus_tag="VQ846_000298"
     CDS             7895..8506
                     /locus_tag="VQ846_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET3074948.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDFLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYTKFVFGCKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            8597..9301
                     /locus_tag="VQ846_000299"
     CDS             8597..9301
                     /locus_tag="VQ846_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET3074949.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            9520..10893
                     /gene="ftsA"
                     /locus_tag="VQ846_000300"
     CDS             9520..10893
                     /gene="ftsA"
                     /locus_tag="VQ846_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET3074950.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            10910..12169
                     /gene="ftsZ"
                     /locus_tag="VQ846_000301"
     CDS             10910..12169
                     /gene="ftsZ"
                     /locus_tag="VQ846_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET3074951.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            12174..12845
                     /locus_tag="VQ846_000302"
     CDS             12174..12845
                     /locus_tag="VQ846_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET3074952.1"
                     /translation="MNVKENTELVFREVGEASLSAHRESSSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            12855..13394
                     /locus_tag="VQ846_000303"
     CDS             12855..13394
                     /locus_tag="VQ846_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET3074953.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSVGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            13394..13657
                     /locus_tag="VQ846_000304"
     CDS             13394..13657
                     /locus_tag="VQ846_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET3074954.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            13654..14439
                     /locus_tag="VQ846_000305"
     CDS             13654..14439
                     /locus_tag="VQ846_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET3074955.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELNLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLFQDKGQTKKEKKKITVQLLLSK"
     gene            14448..15236
                     /gene="divIVA"
                     /locus_tag="VQ846_000306"
     CDS             14448..15236
                     /gene="divIVA"
                     /locus_tag="VQ846_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET3074956.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            15584..18376
                     /locus_tag="VQ846_000307"
     CDS             15584..18376
                     /locus_tag="VQ846_000307"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET3074957.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPASETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKNLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEAQTFANQEEVLD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAAVSFEDVAFTVERAAGQVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFAEAVAEGFEEK"
     gene            18779..19159
                     /locus_tag="VQ846_000308"
     CDS             18779..19159
                     /locus_tag="VQ846_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074958.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTAVNTVATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDNRVGAAPSVLMIVFGGVSLIPFLGW
                     VGGILVIIGGSLFLATLKKFKSEE"
     gene            19353..19643
                     /locus_tag="VQ846_000309"
     CDS             19353..19643
                     /locus_tag="VQ846_000309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074959.1"
                     /translation="MIESIRTYADYIDLYKNIFDDYFTKAEEGFKGTAMENNDSFTKL
                     KESTQKLFDAQKKRLNNEDRIETTKNNVIAKHCQTVLSFLVLTSFFVKNCVK"
     gene            19694..20386
                     /locus_tag="VQ846_000310"
     CDS             19694..20386
                     /locus_tag="VQ846_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET3074960.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            20439..22850
                     /locus_tag="VQ846_000311"
     CDS             20439..22850
                     /locus_tag="VQ846_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET3074961.1"
                     /translation="MIRNKKQDYVLAYKQPVSTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALVTTSYVYKGTRFE
                     REAFASFPDDLLVQRFTKEGAETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEANVDAFTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            23100..23801
                     /locus_tag="VQ846_000312"
     CDS             23100..23801
                     /locus_tag="VQ846_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3074962.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            23803..26511
                     /locus_tag="VQ846_000313"
     CDS             23803..26511
                     /locus_tag="VQ846_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET3074963.1"
                     /translation="MIKRKTYWKDLIQSFTDSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDREIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQAQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGRAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(26607..27098)
                     /gene="tpx"
                     /locus_tag="VQ846_000314"
     CDS             complement(26607..27098)
                     /gene="tpx"
                     /locus_tag="VQ846_000314"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET3074964.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(27221..28150)
                     /gene="psaA"
                     /locus_tag="VQ846_000315"
     CDS             complement(27221..28150)
                     /gene="psaA"
                     /locus_tag="VQ846_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET3074965.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(28182..29030)
                     /locus_tag="VQ846_000316"
     CDS             complement(28182..29030)
                     /locus_tag="VQ846_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET3074966.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(29027..29749)
                     /locus_tag="VQ846_000317"
     CDS             complement(29027..29749)
                     /locus_tag="VQ846_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET3074967.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            30040..31932
                     /gene="pepO"
                     /locus_tag="VQ846_000318"
     CDS             30040..31932
                     /gene="pepO"
                     /locus_tag="VQ846_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET3074968.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(32055..32684)
                     /locus_tag="VQ846_000319"
     CDS             complement(32055..32684)
                     /locus_tag="VQ846_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET3074969.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            33680..35902
                     /locus_tag="VQ846_000320"
     CDS             33680..35902
                     /locus_tag="VQ846_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET3074970.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            35932..36375
                     /locus_tag="VQ846_000321"
     CDS             35932..36375
                     /locus_tag="VQ846_000321"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET3074971.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGFLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(36642..36827)
                     /locus_tag="VQ846_000322"
     CDS             complement(36642..36827)
                     /locus_tag="VQ846_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074972.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRELTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(36846..37259)
                     /locus_tag="VQ846_000323"
     CDS             complement(36846..37259)
                     /locus_tag="VQ846_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074973.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLD
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGILTIDFLFFVLLAFISSQRFSSLMP
                     IITARHSTFYYMTADYLGENYPDFYDKHISLWECL"
     gene            complement(37390..38037)
                     /locus_tag="VQ846_000324"
     CDS             complement(37390..38037)
                     /locus_tag="VQ846_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HET3074974.1"
                     /translation="MVVGIAIASGYLNSRLHKFVDWGLWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWKASQE
                     GRPNQMSQKQVKLAPIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            complement(38063..38254)
                     /locus_tag="VQ846_000325"
     CDS             complement(38063..38254)
                     /locus_tag="VQ846_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074975.1"
                     /translation="MSRKSTNFSSPLPTLKVLFSGFYFYHFILLSFGVRIFEKNGRIF
                     EKKKGSLTYEKITKNFCYI"
     gene            complement(38235..38885)
                     /locus_tag="VQ846_000326"
     CDS             complement(38235..38885)
                     /locus_tag="VQ846_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET3074976.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            38997..39839
                     /locus_tag="VQ846_000327"
     CDS             38997..39839
                     /locus_tag="VQ846_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET3074977.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEDFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            39915..40352
                     /locus_tag="VQ846_000328"
     CDS             39915..40352
                     /locus_tag="VQ846_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET3074978.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            40420..40722
                     /locus_tag="VQ846_000329"
     CDS             40420..40722
                     /locus_tag="VQ846_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3074979.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(40813..41886)
                     /locus_tag="VQ846_000330"
     CDS             complement(40813..41886)
                     /locus_tag="VQ846_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET3074980.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            42042..42719
                     /locus_tag="VQ846_000331"
     CDS             42042..42719
                     /locus_tag="VQ846_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3074981.1"
                     /translation="MHKILLVEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            42712..43686
                     /locus_tag="VQ846_000332"
     CDS             42712..43686
                     /locus_tag="VQ846_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET3074982.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALVFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(43735..45678)
                     /gene="thrS"
                     /locus_tag="VQ846_000333"
     CDS             complement(43735..45678)
                     /gene="thrS"
                     /locus_tag="VQ846_000333"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET3074983.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFIISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVNYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(45736..46059)
                     /locus_tag="VQ846_000334"
     CDS             complement(45736..46059)
                     /locus_tag="VQ846_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET3074984.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENFLSDKEKQLN
                     VRTWK"
     gene            46918..47547
                     /locus_tag="VQ846_000335"
     CDS             46918..47547
                     /locus_tag="VQ846_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET3074985.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     FYIDETKTTRLLDTSDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            47688..47957
                     /gene="rpsO"
                     /locus_tag="VQ846_000336"
     CDS             47688..47957
                     /gene="rpsO"
                     /locus_tag="VQ846_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET3074986.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            48773..49387
                     /locus_tag="VQ846_000337"
     CDS             48773..49387
                     /locus_tag="VQ846_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET3074987.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAILGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(49680..50429)
                     /locus_tag="VQ846_000338"
     CDS             complement(49680..50429)
                     /locus_tag="VQ846_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET3074988.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            50562..52898
                     /locus_tag="VQ846_000339"
     CDS             50562..52898
                     /locus_tag="VQ846_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET3074989.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            53427..54890
                     /locus_tag="VQ846_000340"
     CDS             53427..54890
                     /locus_tag="VQ846_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET3074990.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKV
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFKMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFTELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSVFLTEGDKQRLQAKIQEINYE"
     gene            54883..55311
                     /locus_tag="VQ846_000341"
     CDS             54883..55311
                     /locus_tag="VQ846_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET3074991.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKSPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            55289..55738
                     /locus_tag="VQ846_000342"
     CDS             55289..55738
                     /locus_tag="VQ846_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET3074992.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            55766..56077
                     /locus_tag="VQ846_000343"
     CDS             55766..56077
                     /locus_tag="VQ846_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HET3074993.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            56103..57188
                     /locus_tag="VQ846_000344"
     CDS             56103..57188
                     /locus_tag="VQ846_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HET3074994.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            57199..57894
                     /locus_tag="VQ846_000345"
     CDS             57199..57894
                     /locus_tag="VQ846_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET3074995.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLKSTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            57931..59901
                     /gene="tkt"
                     /locus_tag="VQ846_000346"
     CDS             57931..59901
                     /gene="tkt"
                     /locus_tag="VQ846_000346"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET3074996.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYKDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            60274..>60410
                     /locus_tag="VQ846_000347"
     CDS             60274..>60410
                     /locus_tag="VQ846_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET3074997.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YG"
BASE COUNT        17788 a        11089 c        13717 g        17816 t
ORIGIN      
        1 ccgcttgcca gaatactcag ttgccaaata ctggaacgat attagttctg cagctccaaa
       61 cacagactac gtgatttaca acattcctca attggcaggg gttgctttga ctccaagcct
      121 ttacacagaa atgttgaaaa atcctcgtgt tatcggtgtt aagaactctt ctatgccagt
      181 tcaagatatc caaacctttg tcagccttgg cggggaagac catattgtct ttaacggtcc
      241 agatgaacag ttcctaggtg gccgcctcat gggtgctaag gctggtatcg gtggtactta
      301 tggtgctatg ccagaactct tcttgaaact caatcagttg attgcggata aggacctaga
      361 aacagcgcgt gaattgcagt atgctatcaa cgcaatcatt ggtaaactca cttctgctca
      421 tggaaatatg tacggtgtca tcaaagaagt cttgaaaatc aatgaaggct tgaatattgg
      481 atctgttcgt tcaccattga caccagtaac tgaagaagat cgtccagttg tagaagcggc
      541 tgcagccttg attcgtgaaa ccaaggagcg cttcctctaa tctaaaagga ggtatttatg
      601 acatactacg ttgcaattga tatcggtgga actaacatca agtatggttt ggttgatcaa
      661 gaggggcaac ttcttgaatc gcatgaaatg ccaactgagg cgcataaggg tggacctcat
      721 atcttacaaa agaccaaaga tatcgtagct agttatttag aaaaaggccc agtagcaggt
      781 gttgccatat cttctgctgg gatggtggat ccggataagg gtgagatttt ctatgctggg
      841 ccgcaaatcc ctaactacgc aggcacccag ttcaaaaagg aaatcgaaga aagctttact
      901 attccttgtg agattgaaaa tgatgtcaac tgtgcaggtc ttgctgaggc agtatctggt
      961 tcaggcaagg gagcaagtgt gacactttgc ttgaccattg gaacaggtat cggtggttgc
     1021 ttgattatgg ataggaaagt cttccatggt tttagtaatt cagcctgtga agtcgggtat
     1081 atgcatatgc aggatggagc ttttcaagac ttggcttcta caacagcttt agtgaaatat
     1141 gtagctgaag cccatggaga agatgttgat cagtggaatg gccgacgtat ctttaaggaa
     1201 gctactgaag gaaacaaaat ctgcatggaa ggtattgacc gtatggttga ctatctagga
     1261 aaaggtctgg caaatatttg ctacgttgcc aatccagaag tggttattct tggtggtggt
     1321 atcatggggc aagaggctat cctcaaacct aagatccgca cagccttgaa agaggctttg
     1381 gtaccaagtt tagcagaaaa aacacgatta gaatttgccc atcaccaaaa tacagcaggg
     1441 atgttgggtg catattatca ttttaagaca aaacaatcct agtttggctc agccaaacta
     1501 ggattttctt acacgttttt gtctacgata gccgttgagt tttttatttt cccagtagct
     1561 attaaagatt ttttccttgc tttcgcgatt gatttccaaa aagtaggcat aaatcaaatc
     1621 gataaagaag agcataggaa gttgagcgga tattcgttgg atataggagg gttggctgtg
     1681 ggtggctaca agaacagtct ctgtataggt ctggctatct ttattgggaa cacttgtaaa
     1741 gagtacagtc tttgccccca tctccttggc atctaataga ctatctaaaa tagaaggagt
     1801 tgagcctgaa agtgagaagc ccagtactag acaattttca tccatgatgc tggttgtcca
     1861 ggcaaagccg tcttggtctg tcaaagcttc gcagaccaca cctagtcgca taaaacgtaa
     1921 tttcatttca cgggcgacga ggccagaact ccctgttccg aagaagtaga tacgctcagc
     1981 atcttcgatt agctgggcaa ttcgttctag ttggatttcg tcaatcaagt cttgtgtttg
     2041 ttccctcatg ttgctataac ttctgaggac tcgtttggtc agtggactgt gcttggagac
     2101 ttggttggct tgattttctg cctgatgttg gtattggaaa ataaattctc ggtagccagt
     2161 aaagccacac tttttagcaa agcgggtcaa agcagcttga gaaatatgta atttttgggt
     2221 gacttgttga gaagataaat catctgtaat cgtttcagct tgcaaaaaat agcgagcgat
     2281 ttcttgttct aggtctgtca tttcttcaaa atgtgaatca atgatagttg cgatatctgg
     2341 tttgtccata gggaaagctc ctttacatga gtcatactgg aagactagat cagggaatag
     2401 tcacacttca ttataacaca taatataagg atagataaat aaaaacgcat ctctgtttta
     2461 aaaacgaaaa aatcgaaaaa gcttctctct tttccataat tttctactca aattgtggta
     2521 caattaagag taagatttta agttagaaat gagactgatt tgtatgagaa aatttaacag
     2581 ccattcgatt ccgattcggc ttaatttatt gttttcaatc gtcattttac tctttatgac
     2641 cattattggt cgtttgttgt atatgcaggt tttgaacaag gatttttacg aaaaaaagct
     2701 agcctcagct agtcagacca agattacaag cagttcagcc cgtggggaaa tttatgatgc
     2761 tagtggaaaa cctttggtag aaaatacgtt aaagcaggtt gtttccttta cgcgtagcaa
     2821 taaaatgaca gctacagact taaaagaaac agctaaaaag ttactgactt atgtgagcat
     2881 cagttctcca aatttgacag aacgccagct ggcggattac tatttggctg atcctgaaat
     2941 ctataaaaaa acagtggaag ctctcccaag cgagaaacgc ttggattcag atggcaatcg
     3001 tctatccgaa tcagaactgt ataacaatgc ggtcgatagt gtacaaacga gtcaactaaa
     3061 ctatacagag gatgaaaaga aagaaatcta tctttttagt cagttaaatg ctgttggaaa
     3121 ctttgcgaca ggaaccattg cgacagatcc tctaaatgat tctcaggtgg ctgttattgc
     3181 ctctatttca aaggagatgc ctggcattag tatttctact tcttgggata gaaaggtttt
     3241 ggaaacttcc ctttcttcta tagttgggag tgtatccagt gaaaaagctg gtctcccagc
     3301 ggaagaagca gaagcctatc ttaaaaaagg ctattctcta aatgatcgtg taggaacttc
     3361 ctatttggaa aagcaatatg aagagacctt acaaggaaaa cgctcggtaa aagaaatcca
     3421 tctggataaa tatggcaata tggaaagcgt ggatacaatt gaggaaggta gtaagggaaa
     3481 caatatcaaa ctgaccattg atttggcttt ccaagatagc gtggatgctt tactgaaaag
     3541 ttatttcaat tccgagctag aaaatggtgg agccaagtat tctgaaggtg tctatgcagt
     3601 cgcccttaac ccaaaaacag gtgcggtttt gtctatgtca gggattaaac atgacttgaa
     3661 aacgggagag ttgacgcctg attccttggg aacggtaacc aatgtctttg ttccaggttc
     3721 ggttgtcaag gcggcgacca tcagctcagg ttgggaaaat ggagtcttgt caggaaacca
     3781 gaccttgaca gaccagtcca ttgtcttcca aggttcagct cccatcaatt cttggtatac
     3841 tcaggcttac ggttcattcc ctatcacagc ggtccaagct ctggagtatt catcaaatac
     3901 ctatatggtc caaacagcct taggtcttat ggggcaaacc tatcaaccca atatgtttgt
     3961 cggcaccagc aatctagagt ctgctatgga gaaactgcgt tcaacctttg gcgaatatgg
     4021 cttgggtact gcgacaggaa ttgacctacc agatgaatct actggatttg ttcccaaaga
     4081 gtatagcttt gctaattaca ttactaatgc ctttgggcag tttgataact atacgccgat
     4141 gcagttggct cagtatgtag caactattgc aaataatggt gttcgtgtgg ctcctcgtat
     4201 tgttgaaggc atttatggta ataatgataa gggaggactg ggtaacttga ttcagcaact
     4261 gcaaccgaca gagatgaata aggtcaatat atccgactcc gatatgagca tcttgcacca
     4321 aggtttttat caggttgccc atggtactag tggattgaca actggacgtg ccttttcaaa
     4381 tggtgccttg gtatccatta gcggaaaaac aggtacagcc gaaagctatg tggcagatgg
     4441 tcagcaagca accaatacca atgcggtggc ctatgcccca tctgataatc cccaaatcgc
     4501 tgtcgcagtg gtctttcctc ataataccaa tctaacaaat ggtgtaggac cttccattgc
     4561 gcgtgacatt atcaatctgt atcaaaaata ccatccaatg aattagaaag gaaattatgc
     4621 tttatccaac acctattgcc aagttgattg acagttattc taagttacca ggtatcggga
     4681 ttaagacggc tacgcgtctg gccttttata cgattgggat gtctgctgat gatgtcaatg
     4741 aatttgcaaa aaatctcctt tctgctaaga gagaattgac atattgttct atttgtggac
     4801 gtttgacaga cgacgatcct tgttctatct gtactgatcc gactcgtgac cagacaacaa
     4861 ttttagttct tgaggatagt agagatgtgg cagccatgga aaatatccaa gaataccatg
     4921 gactctatca tgtccttcat ggcctcattt ctcctatgaa tggtatcagt ccggacgata
     4981 tcaatctcaa gagccttatg actcgtctta tggatagtga ggtttcagaa gtgattgtgg
     5041 cgactaatgc tacagcggat ggtgaagcga cttccatgta tctttcacgt ttgctcaagc
     5101 cggctggtat caaggttacg cgtctagcac gaggtctcgc tgtgggagcg gacattgagt
     5161 atgcggacga agtgacactc ttacgagcga ttgaaaatcg gacagagttg taagtgtagg
     5221 caaatttacg aactccattc atttataaaa aatcaaagag gctgaaaatc gttcctatcg
     5281 gcctcttttt gtatagtgtg atgagtagga tcaggttcaa gttttaaaaa accaagcaaa
     5341 tatgatatac taaagagcga gtattctagt agaattagga caaataagta gaattaggac
     5401 aaataatatg aaacaaacga ttattctttt atatggtgga cggagtgcgg aacgcgaagt
     5461 ctctgtcctt tcagctgaga gtgtcatgcg tgcggtcaat tacgaccgtt tcacagtcaa
     5521 gactttcttt atcagtcagt caggtgactt tatcaaaaca caggaattta gtcatgctcc
     5581 ggggcaagaa gaccgtctca tgaccaatga aaccattgat tgggataaga aagttgcacc
     5641 aagtgctatc tacgaagaag gtgcagtggt ctttccagtc cttcacgggc caatgggaga
     5701 agatggctct gttcaaggat tcttggaagt tttgaaaatg ccttacgttg gttgcaacat
     5761 tttgtcatca agtcttgcca tggataaaat cacgactaag cgtgttctgg aatctgctgg
     5821 tattgcccaa gttccttatg tggctatcgt tgaaggcgat gatgtgactg ctaaaatcgc
     5881 tgaagtggaa gaaaaattgg cttatccagt cttcgctaag ccgtcaaaca tggggtctag
     5941 tgtcggtatt tctaagtctg aaaaccaaga agaactccgt caagccttaa aacttgcctt
     6001 ccgatatgac agccgtgtct tggttgagca aggagtgaat gcccgtgaaa ttgaggttgg
     6061 cctcttgggt aactacgatg tcaagagcac gctacctgga gaagttgtca aggacgttgc
     6121 cttttatgac tacgatgcca agtatattga taacaagatt actatggata ttcctgccaa
     6181 aatcagtgat gatgtggtgg ctgtcatgcg tcaaaatgca gaaacagcct tccgtgccat
     6241 tggtggcctt ggtctatctc gttgcgattt cttctataca gataagggag agatttttct
     6301 caacgagctc aataccatgc caggtttcac ccagtggtct atgtacccac tactttggga
     6361 caatatgggg atcagctacc cagaactaat cgagcgtttg gttgaccttg ccaaggaaag
     6421 ttttgacaag cgcgaagcgc atttgatata aaatgaaaga gagggtagaa gccagaacca
     6481 tcactgcaag gtgactagag ttctcggact tcgccccttt ttaaaggagt agaaatgaaa
     6541 ttaacaatct atgaagttgc ccaagctgtt ggagctaaaa atgatgttag tctttttgcg
     6601 gacgcccagt tagaaaaggc tgagtttgac agtcgattga ttgggactgg agatttattt
     6661 gtgccactta aaggtgcgcg tgatggccat gactttattg aaacagcctt tgaaaatggt
     6721 gcagcagtaa ccttgtctga gaaagaggtc tcaaatcatc cttacattct agtagatgat
     6781 gttttgacag cctttcaatc cttagcatcc tactatcttg aaaaaacgac tgttgatgtc
     6841 tttgctgtta caggttcaaa tggcaagaca acgactaagg atatgttggc gcatttactg
     6901 tcaacaagat acaagaccta caaaacacaa ggcaattaca ataatgagat tggccttcct
     6961 tacacagttc ttcatatgcc tgaaggaaca gaaaagttgg ttttggagat gggacaggat
     7021 cacttgggag atattcatct cttgtctgaa ttggctcgtc caaaaacagc catcgtgacc
     7081 ttggttggag aagctcattt ggcctttttc aaagatcgtt ctgagattgc taaaggaaaa
     7141 atgcaaattg cagacggaat ggcttcaggt tccttgcttt taacgccggc tgaccctatc
     7201 gtagaggact atttgccaac tgataaaaag gtggtccgtt ttgggcaagg ggcagagttg
     7261 gaaatcacag acttggttga gcgtaaggac agtctgacct ttaaggctaa tttcttggaa
     7321 caagcccttg atttgccagt gactggtaag tacaatgcta ccaatgctat gattgcgtcc
     7381 tatgttgccc tacaagaagg agtttcagag gagcaaattc atcaggcctt ccaaggtctt
     7441 gaattgacgc gtaatcgtac tgagtggaag aaagcagcca atggagcaga tatcctgtca
     7501 gacgtatata atgccaatcc aactgctatg aagctgattt tagagacttt ctctgccatt
     7561 ccagccaatg aaggtggcaa gaaaattgca gtgttggcgg atatgaagga gcttggtgac
     7621 cagtctattc aactccataa ccagataatt ttgagccttt ctccagatgt gcttgatacc
     7681 gttattttct atggagaaga cattgctgaa ttagcccaac ttgctagtca aatgttccca
     7741 atcggacacg tttattactt caagaaaaca gaagaccaag accaatttga agacctagtt
     7801 aagcaggtca aagaaagcct tggggctcat gaccaaatcc tgctcaaagg ctctaactct
     7861 atgaatctag ccaagttggt agaaagttta gaaaatgaag acaagtgatt ttgtcaagta
     7921 tttgcaaaga atgattgcca ttacagatac tggcttaacc tttacaaaag atccgtttga
     7981 ccgtgagcgc tacgaagact tgcgagactt tttatctgaa atgttgaatc aagcatcaga
     8041 ccttgattcc gaagaagtgg cagaagtctt gaagccaact tctgcttatg cgactccgtt
     8101 aatggacgtc cgtgcttgga ttgttgagga cgaaaagatt tgtctggtta ggggacaagg
     8161 agaggatagt tgggctttgc caggtggctt tggtgaggtt ggctattctc caacagaaaa
     8221 tattctcaag gaaattgaag aagaaaccgg ttttaaagcc aaagttgaaa gactgctagc
     8281 tgtttttgat accaatcgtt tccaactaca gagcaaacag tatacaaagt ttgtttttgg
     8341 atgtaagctt ctcgatggac aattccaaga aaatcaagaa attgctgacc ttcaattttt
     8401 tgccattgac caactgccga acttatctga aaaacgcatt accaaggagc aaatagagct
     8461 tctttggcag gtttatcaag gtcatagggg gcaatatctt gactaagaag atgattatcg
     8521 tatttctaaa tccattttta acaactagca tggtataata atatgcagga aaattttgaa
     8581 ttatgaggaa gactagatga atttatggga tattttcttt acgactcagg caaccgagcc
     8641 gcccaaattt gacctttttt ggtatgttag cctatttacg ctcttagcct taacctttta
     8701 tacagcccat cgctatcgtg aaaagaaggt ttaccaacga tttttccaaa tcttgcagac
     8761 tgttcagtta atccttcttt atggttggta ctgggttaat catatgccac tgtcagaaag
     8821 cctacccttt taccattgcc gtatggctat gtttgtggta ctcttgcttc ctggtcagtc
     8881 caaatataaa caatactttg cattattggg aacatttggg acattagcag cctttgttta
     8941 tccagtgcca gatgcttacc cttttccaca tattaccatt ttatccttta tctttggtca
     9001 tttagcactc ttggggaact ctctagttta tctattgaga cagtataatg cgcgattgct
     9061 ggatgtgaag ggaatttttc tcatgacctt tgccctaaat gccttgattt ttgtggtcaa
     9121 tttggtgaca ggtggcgatt acggattttt gacaaaaccg ccattggttg gggatcacgg
     9181 tctagtagct aattatttac ttgtttcaat tgtgctggta gctactatca gtttgactaa
     9241 gaaaatctta gaattctttt tagctcaaga agcagaaaaa atgattgcaa aggaagctta
     9301 acacagagct ttcttttttg ctcttagaga gtttttacaa gcagcttata aaataagaat
     9361 ttctgaatag acaaactcaa aaaatggctg ggaaatttag gaaaaaagca agcacgatta
     9421 aattttttgt gttataatat tttgtgaata gctatgccta tgtttagcta tggaataata
     9481 cgaagtgcga aacttggaag atagagagga agcgatgtaa tggctagaga aggctttttt
     9541 acaggtctag atattggaac aagctctgtc aaggtgcttg tggccgagca gagaaatggt
     9601 gaattaaatg taattggcgt gagtaatgcc aaaagtaaag gtgtaaagga tggaattatt
     9661 gttgatattg atgcagcagc aactgctatc aagtcagcca tttcccaagc ggaagaaaag
     9721 gcaggcattt cgattaaatc agtgaatgtc ggcttgcctg gtaatctttt gcaggtagaa
     9781 ccaactcagg ggatgattcc agtaacatct gatactaagg aaattacgga tcaagatgtt
     9841 gaaaatgttg tcaaatcagc tttgacaaag agtatgacac ctgaccgtga agtcattacc
     9901 tttattcctg aagaatttat tgtggatggt ttccaaggga ttcgtgaccc acgtggcatg
     9961 atgggggttc gccttgaaat gcgtggtttg ctttatacag gacctcgtac tatcttgcac
    10021 aatttgcgta agacggttga gcgtgcaggt gttcaggttg aaaatgttat catttcacca
    10081 ctagcaatgg ttcagtctgt tttgaacgaa ggggaacgtg aatttggtgc tacagtgatt
    10141 gatatggggg caggtcaaac gactgtcgct acaatccgta atcaagaact ccagttcaca
    10201 catattctcc aagaaggtgg agattatgta actaaagata tctccaaggt tttgaaaacc
    10261 tctcgcaaat tagcggaagg cttgaaactg aattacgggg aagcctatcc gcctcttgca
    10321 agcaaagaaa ccttccaagt agaggttatt ggagaagtag aagcagtcga agtgacggaa
    10381 gcctacttgt cagaaattat ttctgcacga atcaagcaca tccttgaaca aatcaagcaa
    10441 gaattagata gaaggcgtct attggacctc cctggtggta ttgtcttaat cggtgggaat
    10501 gccattttac caggtatggt tgagcttgct caggaagtct ttggcgtccg tgtcaagctt
    10561 tatgtcccaa atcaagttgg tatccgtaat ccagcctttg cgcatgtgat tagtttatca
    10621 gaatttgcgg gtcaattaac agaagttaat cttttggctc agggagcgat aaaaggtgag
    10681 aatgacttaa gtcatcagcc aattagtttt ggtgggatgc tgcaaaaaac agctcagttt
    10741 gtacaatcaa cgcctgttca accagctcct gctccagaag tagagccggt ggcgcctaca
    10801 gaaccaatgg cggatttcca acaagcttca caaaataaac cgaaattagc agatcgtttc
    10861 cgtggcttga tcggaagcat gtttgacgaa taaagaggaa aaataaatta tgacattttc
    10921 atttgataca gctgctgctc aaggggcagt gattaaagta attggtgtcg gtggaggtgg
    10981 tggcaatgcc atcaaccgta tggtcgacga aggtgttaca ggcgtagaat ttatcgcagc
    11041 aaacacagat gtacaagcat tgagtagtac aaaagctgag actgttattc agttgggacc
    11101 taaattgact cgtggtttgg gtgcaggagg tcaacctgag gttggtcgta aagccgctga
    11161 agaaagcgaa gaaacactga cggaagctat tagtggtgcc gatatggtct tcatcactgc
    11221 tggtatggga ggaggctctg gaactggagc tgctcctgtt attgctcgta tcgccaaaga
    11281 tttaggtgcg cttacagttg gtgttgtaac acgtcccttt ggttttgaag gaagtaagcg
    11341 tggacaattt gctgtagaag gaatcaatca acttcgtgag catgtagaca ctctattgat
    11401 tatctcaaac aacaatttgc ttgaaattgt tgataagaaa acaccgcttt tggaggctct
    11461 tagcgaagcg gataacgttc ttcgtcaagg tgttcaaggg attaccgatt tgattaccaa
    11521 tccaggattg attaaccttg actttgccga tgtgaaaacg gtaatggcaa acaaagggaa
    11581 tgctcttatg ggtattggta tcggtagtgg agaagaacgt gtggtagaag cggcacgtaa
    11641 ggcaatctat tcaccacttc ttgaaacaac tattgacggt gctgaggatg ttatcgtcaa
    11701 cgttactggt ggtcttgact taaccttgat tgaggcagaa gaggcttcac aaattgtgaa
    11761 ccaggcagca ggtcaaggag tgaacatctg gctcggtact tcaattgatg aaagtatgcg
    11821 tgatgaaatt cgtgtaacag ttgttgcaac gggtgttcgt caagaccgcg tagaaaaggt
    11881 tgtggctcca caagctagat ctgctactaa ctaccgtgag acagtgaaac cagctcattc
    11941 acatggtttt gatcgtcatt ttgatatggc agaaacagtt gaattgccaa aacaaaatcc
    12001 acgtcgtttg gaaccaactc aggcatctgc ttttggtgat tgggatcttc gccgtgaatc
    12061 gattgttcgt acaacagatt cagtcgtttc tccagtcgag cgctttgaag ccccaatttc
    12121 acaagatgaa gatgaattgg atacacctcc atttttcaaa aatcgttaag taaatgaatg
    12181 taaaagaaaa tacagaactt gtttttcgag aagttggaga ggctagtctg agtgctcatc
    12241 gagagagtag ttcggtctct gtcattgcag ttaccaagta tgtagatgta ccgacagcgg
    12301 aagccttgct tccgctaggt gttcatcata tcggtgaaaa tcgtgtagat aagtttctgg
    12361 aaaaatatga agctttaaaa gatcgagatg tgacttggca tttgattggt accttgcaaa
    12421 gacgtaaggt gaaagatgtc attcaatacg ttgattattt ccatgcattg gactcagtaa
    12481 agctagcagg ggaaattcaa aaaagaagtg accgagtcat caagtgtttc cttcaagtaa
    12541 atatttctaa agaagaaagc aaacacggtt tttcgagaga ggaactgctg gaaatcttgc
    12601 cagagttagc cagactagat aagattgaat atgttggttt aatgacgatg gcaccttttg
    12661 aggctagcag tgagcagttg aaagagattt tcaaggcgac ccaagattta caaagagaaa
    12721 ttcaagagaa acaaattcca aatatgccta tgaccgagtt aagtatggga atgagtcgtg
    12781 attataaaga agcgattcaa ttcggttcca cttttgttcg tataggtaca tcatttttta
    12841 agtaggagag aaccatgtct ttaaaagata gattcgatag atttatagat tattttacgg
    12901 aggatgagga ttcaagtctc ccttatgaaa aaagagatga gcctgtgttt actccagtaa
    12961 attcttcaca ggaaccggct ctcccaatga atcaaccttc acagtcggtt ggtacaaaag
    13021 agaacaatat caccagactt catgcaagac aacaggaatt ggcaaatcag agtcagcgtg
    13081 caacggataa ggtcattata gatgttcgtt atcctagaaa atatgaggat gcaacagaaa
    13141 ttgttgattt attggcagga aacgaaagta tcttgattga ttttcagtat atgacagagg
    13201 tgcaggctcg tcgttgtttg gactatttgg atggagcttg tcatgtttta gctggaaatt
    13261 tgaaaaaggt agcttctacc atgtatttgt tgacaccagt gaatgttatt gtaaatgttg
    13321 aagatatccg tttaccagat gaagatcaac agggtgagtt cggttttgat atgaagcgaa
    13381 atagagtacg ataatgattt ttttaattcg tatgatttat aatgcagtgg atatttactc
    13441 cctgattttg gtagccttcg ctgtcatgtc ttggtttcca ggtgcctacg aatccagttt
    13501 aggtcgttgg attgtagcgt tggtgaaacc agtacttgct cccttgcaac gcctgccttt
    13561 acagatagcg ggtcttgatt tatctgtttg ggtcgcgatt gttttggttc gatttttagg
    13621 agaaaaccta gtgcgttttc tggcgatgat aggatgaata aagggattta tcagcatttc
    13681 tccatagaag atcgtccatt tcttgacaag ggaatggaat ggataaagaa ggtagaagat
    13741 agctatgctc cttttttaac tccttttatc aatcctcatc aggagaagct attaaagatt
    13801 ttggccaaaa cctatggtct tgcttgtagc agtagtgggg aattcgtctc gagtgagtat
    13861 gttcgagttt tattataccc agattatttc caaccagagt tttcagattt tgaaatatct
    13921 ctccaagaaa ttgtgtattc caataaattt gaacatttaa cgcatgctaa gattttaggg
    13981 acagtcatca atcaattagg gattgaacgg aaactttttg gagatatcct agtagatgaa
    14041 gaacgggcgc agattatgat taatcagcag tttcttcttc tctttcaaga tggactaaag
    14101 aaaattggtc gtatacctgt ttcgctggag gaacgtcctt tcaccgagaa aatagataag
    14161 ctagaacagt atcgagaact gaatttatct gtgtctagtt ttcgattaga tgttctttta
    14221 tcaaatgttt tgaaactatc taggaatcaa gcaaaccagt tgattgaaaa gaaacttgtc
    14281 caagtaaatt atcatgtggt agacaaatca gattacactg ttcaagttgg agacttgatt
    14341 agtgtgagaa aatttggtcg cttgagatta tttcaagata agggacaaac gaaaaaagag
    14401 aagaaaaaaa taaccgtcca gttattatta agtaagtgag gaatagaatg ccaattacat
    14461 cattagaaat aaaggacaag acctttggaa ctcgattcag aggttttgat ccagaagaag
    14521 tcgatgaatt tttagatatt gtggttcgtg attacgaaga tcttgtgcgt gcgaatcatg
    14581 ataaaaattt gcgtattaag agtttagaag agcgtttgtc ttactttgat gaaataaaag
    14641 attcattgag ccagtctgta ttgattgccc aggatacagc tgagagagtg aaacaggcgg
    14701 cgcatgaacg ttcaaacaat atcattcatc aagcagagca agatgcgcaa cgcttgttgg
    14761 aagaagctaa atataaggca aacgagattc ttcgtcaagc aactgataat gctaagaaag
    14821 tcgctgttga aacagaagaa ttgaagaaca agagccgtgt cttccaccaa cgtctcaaat
    14881 ctacaattga gagtcagttg gctattgttg aatcttcaga ttgggaagat attctccgtc
    14941 caacagctac ttatcttcaa actagtgatg aagcctttaa agaagtggtt agcgaagtac
    15001 ttggagaacc gattccagct ccaattgaag aagaaccaat tgatatgaca cgtcagttct
    15061 ctcaagcaga aatggaagaa ttacaagctc gtattgaggt agccgataaa gaattgtctg
    15121 aatttgaagc tcagattaaa caggaagtgg aaactccaac tcctgtagtg agtcctcaag
    15181 ttgaagaaga gcctctgctc atccagttgg cccaatgtat gaagaaccag aagtagctcc
    15241 agtgcatccg acaggtccaa caccagctac agaaactgtt gattcagcat caggatttga
    15301 agcaccgcaa gaatccgtta caattttata agaaacgatg tgagaacaat atcttatcct
    15361 tatatttcca gcgagcagga gatggtgtga gtcctgcatt ccctaacgat aagattatcc
    15421 tctcaaaaac tcaagtctga agctagtaag atttgacgtt tcccacgtta cgggataaga
    15481 gggagaaaga ctaaatcttt ttccgaataa aggtggtacc acgattttcg tcctttttgg
    15541 aagtcgtggt ttttaatttg ttattattta taaaggagat accatgaaac tcaaagatac
    15601 ccttaatctt gggaaaacag ctttcccaat gcgtgcaggc cttccgacca aagagccagt
    15661 ttggcaaaag gaatgggaag atgcaaaact ttaccaacgt cgtcaagaac taaatgaagg
    15721 gaaaccgcat tttgtcttgc atgatggccc tccatacgca aacggaaata tccacgttgg
    15781 acatgccatg aatcatatct caaaagatat catcattcgt tctaagtcta tgtcaggttt
    15841 taacgcacct tatatcccag gttgggatac tcatggtctg ccaatcgagc aagttttggc
    15901 aaagcaaggt gtgaagcgca aagaaatgga cttggttgag tacttgaaac tctgtcgtga
    15961 gtacgctctt tctcaagttt ataaacaacg tgatgatttc aaacgcttgg ggatgtcagg
    16021 tgactgggaa aatttatatg tgactttaac tccagactat gaagcagccc aaatccgtgt
    16081 ctttggtgag atggctaata agggttatat ctaccgtggt gccaagccag tttactggtc
    16141 atggtcatct gagtcagccc ttgccgaagc ggaaattgaa taccatgatt tggtttcgac
    16201 ttcactttat tatgccaata aggtaaaaga tggcaaaggt atcctagata cagatactta
    16261 tatcgtagtc tggacaacaa ctccatttac catcacagct tctcgtggtt tgactgttgg
    16321 tgcagatatt gattacgttt tggttcaacc tgctagtgaa actcgtaagt ttgtcgttgc
    16381 tgcagaattg ttgactagct tgtctgagaa atttggctgg gctgatgttc aagtgttggc
    16441 aacttaccgt ggtcaagaac tcaaccacat cgtaacagaa cacccatggg atacagctgt
    16501 agatgagttg gtaatccttg gtgatcacgt taccactgac tctggtacag gtatcgtcca
    16561 tacagcccct ggttttggtg aggacgacta caatgttggt attgctaatg gtcttgaagt
    16621 cgcagtgact gttgacgaac gcggtatcat gatggcgaat gctggtcctg agtttgaagg
    16681 tcagttctac gacaaggttg taccaactgt tattgaaaaa cttggtaacc tccttctagc
    16741 ccaagaagaa atctctcact catacccatt tgactggcgt actaagaaac caatcatctg
    16801 gcgtgcagtt ccacaatggt ttgcctcagt ttcaaaattc cgccaagaaa tcttggatgc
    16861 tattgataaa gttaaattcc atacagaatg gggtaaagta cgtctatata atatgattcg
    16921 tgaccgtggt gactgggtta tctctcgtca acgtgcttgg ggtgttccac ttccaatctt
    16981 ctatgcagaa gacggtacag ctatcatgac agctgagact attgaacatg tggcgcaact
    17041 ctttgaagta catggttcaa gcatttggtg ggaacgtgat gccaaaaacc tcttaccaga
    17101 aggatttaca catccaggtt ctccaaatgg ggagttcaag aaagaaacag atattatgga
    17161 tgtatggttt gactcaggtt catcatggaa tggtgttttg gtaaatcgtc caaatctgac
    17221 ttatccagct gacctttacc ttgaaggttc tgaccaatac cgtggttggt tcaactcatc
    17281 acttattaca tctgttgcca accatggcgt agcaccttac aaacaaatct tgtcacaagg
    17341 ttttaccctt gatggtaaag gtgagaagat gtctaaatca cttggaaata ccattgctcc
    17401 aagtgatgtt gaaaaacaat ttggtgcaga aatcttgcgt ctctgggtaa caagtgttga
    17461 ctcaagcaat gacgtgcgta tctctatgga tattttgagc caagtttctg aaacttaccg
    17521 taagattcgt aacactcttc gtttcttgat tgccaataca tctgacttta acccagctca
    17581 agatgtagtc gcttacgatg aacttcgttc agttgataag tacatgacta ttcgctttaa
    17641 ccagcttgtc aagaccattc gtgatgccta tgcggacttt gaattcttga caatctacaa
    17701 ggccttggtg aactttatca acgttgactt gtcagccttc taccttgatt ttgccaaaga
    17761 tgttgtttac attgaaggtg ccaaatcatt ggaacgccgt caaatgcaga ctgttttcta
    17821 tgacattctt gtcaaaatca ccaaactctt gacaccaatc cttcctcaca ctgcggaaga
    17881 aatctggtca tatcttgagt ttgaagctga agacttcgtt caattgtcag aattgccaga
    17941 agctcaaact tttgctaatc aagaagaagt cttggataca tgggcagcct tcatggactt
    18001 ccgtggacaa gctcaaaaag ccttggaaga agctcgtaat gcaaaagtta tcgggaaatc
    18061 gcttgaagca cacttgacag tttatccaaa tgaagttgtg aaaactctac tcgaagcagt
    18121 aaacagcaat gtggctcaac ttttgatcgt gtcagacttg accatcgcag aagggccagc
    18181 tccagaagct gctgttagct tcgaagatgt agccttcaca gttgaacgcg ctgcaggtca
    18241 agtatgtgac cgttgccgtc gtatcgaccc aacaacagca gaacgcagct accaggcagt
    18301 catctgtgac cactgtgcaa gcatcgtaga agaaaacttt gcggaagcag tcgcagaagg
    18361 atttgaagag aaataaggtt gaaaagtcta ggcaaaattc aatttgagaa gaaaagacaa
    18421 ctaattttat agtctattaa acgcattgta tcacgttttt gaatacctga tatgatgcgt
    18481 tttttattta ttttaaaaat ttgcgaggta tgacttttta tactcaacaa gaatcaaaga
    18541 gaaacttagc aagctaacag tagtaagata aaataggaat ttgatattag ggataagatt
    18601 gctaaatagt gtaatatttt tacaataata aatttatata gttatttttg gtttctgaaa
    18661 agtattatat tttatttcat attatacaaa tttttatttt ataatatcag aaaatacttt
    18721 ttttaaaagc aaatatgata caattttatt tgaaaaaaat aaaaaaggag attttattat
    18781 gaaattaaaa acacttgctt taattagtgg tatcgtcggt cttgtgggag gaattttact
    18841 tcttattggt ccttttgtct tgttgggaac agcggtaaac acagttgcta caactcttaa
    18901 tggaggagct actgcagggg ctttttcagg tgttgcctta ctcttgaatg ccttgaagat
    18961 tgcaaatctt gttcttggta tcattgctat tgtttactat aaaggagata atcgtgtagg
    19021 tgcagctccg tctgtactaa tgattgtttt tggtggagtt agtctcattc cattcttagg
    19081 atgggttggg gggattcttg ttattatcgg aggatctcta ttccttgcaa cattgaagaa
    19141 attcaaatca gaagaataaa aggtatttta gcatgaaaag aacaaaaaag tttatcggta
    19201 taggagtagc tctattatct ctttttcttc tagttgcatg tggaacataa agttcaaagg
    19261 atacttcaac aagtaatgat gagaagacag tagcaacatc caatagttca aaagaaacaa
    19321 tcactttcga tacaccggtt gtaacagacg atgtgattga atcaatacgc acttatgcag
    19381 attatataga tctttataaa aatatttttg atgattattt tactaaagct gaggaaggtt
    19441 tcaaaggcac agctatggaa aataatgact cgtttactaa actaaaagag tcaactcaaa
    19501 aattattcga tgcgcagaaa aaaaggttaa ataatgaaga tagaatagaa acaactaaaa
    19561 acaatgtgat tgccaaacat tgtcaaacag tcctttcctt tttggttttg actagctttt
    19621 ttgtgaaaaa ttgtgtaaaa tagaatagat aaacgagggg aaacctcgga aaatttaaag
    19681 gagaatccat ctaatggtaa aattggtttt tgctcgccac ggtgagtctg aatggaacaa
    19741 agctaacctt ttcactggtt gggctgatgt tgatttgtct gaaaaaggta cacaacaagc
    19801 gattgacgct ggtaaattga tcaaagaagc tggtatcaaa tttgaccaag cttacacttc
    19861 agtattgaaa cgtgctatca aaacaactaa cttggctctt gaagcttctg accaattgtg
    19921 ggttccagtt gaaaaatcat ggcgcttgaa cgaacgtcac tacggtggtt tgactggtaa
    19981 aaataaagct gaagctgctg aacaatttgg tgatgagcaa gttcacatct ggcgtcgttc
    20041 atacgatgta ttgcctccaa atatggaccg tgatgatgag cactcagctc acacagaccg
    20101 tcgttatgct tcacttgacg actcagtaat cccagatgct gaaaacttga aagtgacttt
    20161 ggaacgtgct cttccattct gggaagataa aatcgctcca gctcttaaag atggtaaaaa
    20221 cgtattcgta ggagctcacg gtaactcaat ccgtgccctt gtaaaacaca tcaaaggttt
    20281 gtcagatgac gagatcatgg acgtggaaat ccctaacttc ccaccattgg tattcgaatt
    20341 cgacgaaaaa ttgaacgtcg tttctgaata ctaccttgga aaataaaaaa ttgtaagtct
    20401 agaattgatt tctaggcttt ttatgttagt atggaagtat gataaggaat aaaaaacaag
    20461 attatgtact ggcctacaag caaccagttt caaccactta catgggttgg gaagaagaag
    20521 ctttaccgat aggcaatggt tctttaggag caaaagtatt tggccttata ggggctgaac
    20581 ggattcaatt taatgaaaaa agtctctggt ctggaggtcc acttcctgat agttcagatt
    20641 atcagggtgg aaatcttcag gatcagtatg tttttttagc tgagattcgg caggctttgg
    20701 agaagagaga ttacaatctg gctaaggaac tggctgagca gcacctaatt gggccaaaaa
    20761 cgagtcaata tgggacctat ctgtcttttg gggatattca cattgagttc agccagcaag
    20821 gtacgacttt gtctcaggtg acggactatc agagacagct gaatattagt aaggcacttg
    20881 tgacgacttc ttatgtctat aagggaacgc gatttgaacg tgaagctttt gcgagttttc
    20941 cagatgatct cttggttcag cgttttacta aggaaggggc ggaaactcta gattttacta
    21001 tagaactatc cttgacctgt gatttggctt ctgatggaaa gtatgagcag gaaaaatctg
    21061 attacaagga gtgtaagttg gatattactg attctcatat cttgatgaag ggaagagtta
    21121 aggataatga tctgcggttt gctagttatc tagcttggga aacggatgga gatattagag
    21181 tttggtcaga tagggttcag atatcaggag ccagttatgc caatctcttc ttggccgcta
    21241 agacggattt tgcccaaaat cctgctagca attatcgcaa gaaactagat ttagagcaac
    21301 aggtgataga cttggtggac acagctaaag aaaagggcta tacccaattg aaatcaaggc
    21361 atatcgagga ctaccaagcc ttattccagc gtgttcaatt ggatttggaa gctaatgttg
    21421 acgcattcac tacagatgat ttgttaaaaa attataagcc acaagaaggg caggctttgg
    21481 aggagctgtt cttccagtat ggacggtatt tattgattag ttcgtccaga gactgcccag
    21541 atgctctacc agctaaccta cagggagtct ggaatgcggt cgacaatcct ccttggaatt
    21601 cggactatca cttaaatgtc aatctgcaga tgaattattg gccagcctat gttaccaatc
    21661 tcctagaggc ggtctttccg gtcatcaact atgtagatga tttgcgtgtc tatggtcgtc
    21721 tagcggctgt aaagtatgca ggaatcgtct ctcaaaaagg tgaggaaaat ggttggttgg
    21781 ttcatactca agcgactccc tttggttgga cggcacctgg ttgggattac tattggggtt
    21841 ggtcaccagc tgccaatgcg tggatgatgc aaaccgttta tgaagcctat tcattttata
    21901 gggaccaaga ctatctcagg gagaaaattt atcccatgtt gagggaaacg gttcgttttt
    21961 ggaatgcctt tttacataag gatcagcagg cgcagcgttg ggtgtcttct ccgtcttatt
    22021 ccccagaaca tgggccgatt tcgattggca atacttatga ccaatctctg atttggcagt
    22081 tatttcatga ttttattcag gctgctcagg aattgggact ggatgaggac ttgttgactg
    22141 aggttaagga gaagtctgat ttactaaatc ctttgcaaat cactcaatct ggtcgaatca
    22201 gggagtggta tgaggaggaa gagcagtatt ttcaaaatga gaaagtggag gcccagcatc
    22261 ggcacgcttc ccatctagtg ggactctatc ctggcaatct ctttagctac aagggacaag
    22321 agtatattga agcggcacgt gctagcctca atgatcgtgg agatggcggc acaggctggt
    22381 ccaaggctaa taagatcaat ctctgggcgc gtttgggaga tggcaatcga gcccataaat
    22441 tattggcaga gcagttaaaa acatccacct tgccaaatct ttggtgtagc catcctcctt
    22501 ttcagataga tggtaatttt ggtgctacta gtggcatggc agaaatgtta ctccagtctc
    22561 atgcagctta tctggtacct ctagctgccc tacctgatgc ttggtcaaca ggttctgttt
    22621 caggcttaat ggcacgtgga cattttgaag tgagcatgag ctgggaagat aaaaaactct
    22681 tacagttgac cattttatca aggagtggag gagatttgcg agtttcttat ccagatattg
    22741 agaagagtgt gattaaaatg aatcaagaaa aaataaaagc gaaatgcatg gggaaagatt
    22801 gtatttcggt ggcaacagca gaaggtgatc ttgttcaatt ttatttttaa gaagatgtta
    22861 taaggcagta atttgaaact tccttttaat aaggatttaa gaatataggc agtttccaac
    22921 tagttgaaaa agcgttataa tgataatagg aagtaatact caatgaaaat caaagagcac
    22981 aaactaggaa gctagccgca ggttgctcaa aacagtgttt tgaggttgta gatggaagct
    23041 gacgtggttt gaagagagat tttcgaggag tataatttgt ttgatagagg gtgggtctga
    23101 tggcttatat tgagatgaaa cactgttaca agcgttatca ggttggggac acggagattg
    23161 tggccaatcg tgatgtgaat tttgagattg aaaaggggga gctggttatt atccttggtg
    23221 cttcaggtgc aggcaagtca acagttctta accttcttgg gggaatggat accaatgatg
    23281 aaggggaaat ctggattgat ggtgttaata ttgcggatta tagttcccac cagcgcacca
    23341 attaccgtag aaatgatgtg gggtttgttt ttcagtttta taatctagtt tctaatctga
    23401 cagctaagga aaatgtggaa ctggcttctg aaattgtgac agatgccttg aatcctgatc
    23461 aggtcttgac agatgtaggt ctggctcatc gtctcaataa ctttccagcc cagctttctg
    23521 gaggggagca acagcgagtc tccattgcac gcgcggtagc caaaaatcct aaaattctcc
    23581 tttgtgatga accgactgga gccttggatt atcagacggg caagcaggtt ttgaaaattc
    23641 tccaagacat gtctcgtcaa aagggagcga cggtgatcat cgtgactcat aatggagctt
    23701 tggcgcccat tgctgatcgc gtgattcata tgcacgatgc cagtgtcaag gatgtggtgc
    23761 tcaaccagca tcctcaggat attgacagtt tggagtacta gcatgatcaa gcgaaaaact
    23821 tattggaagg acttaattca gtccttcaca gactccaagg ggcgtttttt atccatcttg
    23881 atcctgatga tgttgggatc tctagcctta gtaggcctca aagtaaccag tcccaacatg
    23941 gaggcgacag ctaatgctta tttaacaact gctcaaacct tggatttggc agtcatgtct
    24001 aactatggct tggatcaagc agaccaagaa gaactaaaac agacggaggg cgcagaggtc
    24061 gagtttggct atttgacaga tgtgactatg gataatgggc aggatgccat tcggctgtac
    24121 tccaaaccag agcgaatttc aacctttcag ctaagaaagg gacgacttcc tcagtcagac
    24181 agggaaatcg ctttggccac tcatttgcaa ggccaataca gcgtgggaca ggagattagt
    24241 tttaaagaaa aagaagaggg tcattcctct ttaaaagacc atacttatac cattactggt
    24301 tttgtggatt cggctgaaat cctctcccag cgagatatgg gctacgcagg aagtggaagt
    24361 gggactctga cagcctatgg ggtgatttta cctagtcaat ttgatcagaa agtctacaat
    24421 atagctcgtt tgaaatatca agatttagca ggtttaaatg ccttttcatc agcttatgaa
    24481 gaaaaatcca agcaacatca ggaagacctt gaacaaactt tatcagataa tggcaaggta
    24541 cgtctgcaac ttttgaaaaa agaaggacaa gagtctctag acaaggggca agagactctt
    24601 gacaaggctc agactaattt gcaggaaggc aagcgtcgtt tagcagctgc tcaagctcgt
    24661 atacaggctc aagaaagtca actagccttg tttcctcaag ctcagagaga gcaggctagt
    24721 gctcaactta cccaagccaa gcaggaactg ggcaaggaag aggacaaact aaagcaagct
    24781 gaacaaaatc tagcccaaga aaaggaaaaa ttagaaaaac atcagcaagt cttggatgat
    24841 ttggcggagc caaggtatca ggtttataat cgtcagacca tgccaggtgg tcagggctat
    24901 cttatgtata gcaatgcttc atccagtatt cgagcagtgg gcaatatctt tcctgtggta
    24961 ctttatgccg tagcagccat ggtgaccttt acgaccatga ctcgctttgt agacgaagag
    25021 cgaactcatg cagggatttt taaggccttg ggttatcgta gtaaggatat tatcgccaag
    25081 tttctccttt atggactagt agctgggact gtcggaacgg ctctaggtag tatacttggt
    25141 cattatttgc tagccagtgt aatttcaagt gtcattacaa aaggcatggt ggtgggagaa
    25201 acccagattc agttctattg gacctatagc ttactagctc ttgtcttgag ctggttggcg
    25261 agtgtgttac cagcctatct ggtggcttgg agggaacttc atgacgaagc agcccagctt
    25321 ctacttccta aacctcctgt caaaggagct aaaatcttat tggagcgtat cggttttatc
    25381 tggcgtcgtc tcagttttac tcataaggta acagcccgca acatctttcg ttataagcag
    25441 agaatgttga tgacaatctt tggtgtggca ggttctgtag ctctgctctt tgcaggtttg
    25501 ggaatccaat cttctgtagc aggagttccg tctaaacagt ttcaacaaat ccaacagtat
    25561 cagatgcttg tctctgaaaa tcctagtgcg accaatcagg acaaggtaga gctagcagaa
    25621 gtgttgaaag ggcaggagat actagcctac cagaaaatct attctaaaac gctagacaag
    25681 gatttcaagg gcagggctgg tcttcaaaac attactctta tgatgataga gaaggaagat
    25741 ttgactccct ttatccatct tcaacatcat cagcaggagc tgacattaaa agatggcatc
    25801 gttattacag ctaaactcgc ccagctggca ggtgtcaagg ttgggcagac tttagaaatt
    25861 gaaggtaagg aactaaaggt cgctgctatt actgagaact acgttggtca ctttatttat
    25921 atgagtcagg ctagctatga gcaactttac ggacagctac cccaagccaa cacttatctg
    25981 gtctcattaa gggataccag tgcaactagt atcgaaagtc aggcgggctt gcttatgaat
    26041 caatctgcgg tgtccagtgt tgtccaaaat gcttcagcca ttcgactctt cgactctatc
    26101 gctagctcac tcaatcagac catgaccatc ttggtcatcg tatcggttct attagctatt
    26161 gtcatccttt acaatctgac caatatcaac gtagctgaga gaatccgtga actctccact
    26221 atcaaggttc tcggttttca taataatgaa gtcaccctct acatttaccg tgagacgatt
    26281 gtgctgtccc ttgtgggaat cgtacttggt ctggtagctg gtttctattt acaccaattt
    26341 ttgattcaaa tgatttcgcc tgcgactatt ctcttttatc cgcaggtagg ctgggaagtc
    26401 tatgtaatcc cagtggcagc agtaagcatc attttgacct tgcttggttt cttcgtcaat
    26461 tattatctga gaaaggttga catgttagaa gccctgaaat ctgtagagta aggtagttat
    26521 ttttagctga ttgaacttct atttactaat attcaaaaat cctccgtttc aaagagcagg
    26581 gaactctttg tgacagagga ttttttctat agggctttag cagctgcaat tgcggcttcg
    26641 aagtttggct cagaattgat attatccacg tattcaacgt agcgaatcgt attgtcagta
    26701 tcgaggacaa agactgcgcg tgctaatagg tgccattcgt tgatcaagag ggcataatcg
    26761 cgtccgaaag aatggtcaaa gtagtctgaa agcataatgg cattgtcaag gccttcagca
    26821 ccgcaccaac gtttttgagc aaaaggtagg tccattgaaa cagtcaatac gaccgtgttg
    26881 tccagtccag ccaattcttc attaaaacga cgtgtttgag ttgagcagat gcctgtatcg
    26941 atagaaggaa cgacactcaa gacttttttc ttgccatcaa aatcagccag agatttttta
    27001 gaaagatctg ttgtagtaag agaaaaatca agcgccttgt cgccgacttg tagttgttta
    27061 cctgtaaagc tcacaggatt tccgagaaaa gttaccatag gatactccaa tcttttttct
    27121 tccattgtat ctgaaacagt cagaattttc caatgatttg accggaaatg tgtgcataga
    27181 aaaaacgcca gctcacatga gaatgacgtt tttcagaggc ttattttgcc aatccttcag
    27241 caatcttgtc aaggttgtat ttcatcatgc tgtagtagct gtcgccttct ttaccttgtt
    27301 ctgcgataga gtcagtaaag atttgtgcgt agattgggat gtttgtgtct tgagaaacag
    27361 ttttcattgg acggtcatcc acacttgatt ctacaaagag tgatggaact tttgtttggc
    27421 gaagtttttc aaccaaggtc ttgatttgtt caggagttcc ttcttcttca gtattgattt
    27481 cccagatgta ggcacttggg acaccatagg ctttagagaa gtatttgaat gctccttcgc
    27541 tggttacaat gagtttcttt tcagcaggga tcttattaaa tttatcctta ctttctttat
    27601 caagtttgtc taacttatca gtatattctt tgagattttt ttcatagaat tctttattgt
    27661 tagggtcttt ggcgctcaat tgtttggcga tatttttagc aaaaataata ccgttttcaa
    27721 ggttaagcca agcgtgtggg tcttcttttc ctttttcatt ttgaccttca aggtagataa
    27781 catcaacgcc gtcgctgact gcgaagtagt ctttgttttc agttttcttg gcattttcta
    27841 ccaattttgt aaaccaagca ttgccacctg tttcaaggtt gataccgtta tagaaaatca
    27901 aatcagcctc agaagttttc ttaacgtctt caggaagtgg ttcgtattcg tgtgggtctt
    27961 gcccaatcgg aacgatacta tgaaggtcaa ttttgtcacc agcaatattt ttagtaatat
    28021 cagcgatgat tgagtttgta gcaacaactt ttagtttttg accagaagtt gtatcttttt
    28081 ttccgctagc acatgctaca agaatgattg cagaaagaaa gagaacgagt aatgtaccta
    28141 attttttcat tagatcctcc aatttattag ggctttgccc cttattttaa caaatgttta
    28201 tttttcagtt tcaaatatcg ttgtttggga gcgataaaga agctaatgag aaagaaacta
    28261 gcagctgtaa gcacgatact agaacctgcc gcaacattaa aactatagcc aataaagagt
    28321 cccaaaactg aagcagtagc tccgaaggtt gaggaaagga aaatcatgct tttcagacta
    28381 ttagcataca gataagcagt tgcagctggg gtaatcagca tggctacaat caggatagtt
    28441 ccgacacttt gcatggctgt cacagacacg agagtcagga gtaccatgag aaggtagtga
    28501 tagaaattga caggcattcc catggcttta gccaagagtt catcaaagga agttatcaag
    28561 agttgcttga agaaaatcca gattaacaag aggatggctg cccccacacc catagtaata
    28621 aacatatccg tatcttggac ggccagaata ttaccaaaaa ggatatggaa aaggtcagtt
    28681 gaacttttag cgacaccaat caagatgata ccgagggcta agaaagaaga aaaggtaatg
    28741 ccgatggcgg tatcgctttt gataatcgag tttcctttga tgtaggtaat gatgatggca
    28801 gctagcaatc caaagacaat ggctccgata aagaagtcaa ggcccaagat gaaggagagg
    28861 gctacacctg gtaagacagc atgtgaaatg gcatctccca tgagtgacat cccgcgtaga
    28921 ataatgaaac atcccacggc tccagctaca atcccgacga caatagctgt tatcaaggca
    28981 ttttgtagga aatggaattt ttgcaatcca tcgataaatt ctgcaatcat aggtcacctc
    29041 cattgaaaaa gagttgatta ccgtaagctt cttttagatt ggtttcggta aaagtttctt
    29101 ttgttggacc aaaggcaatc acttctcgat tgacaagtaa gacttgatcg aagtagtggg
    29161 gaatcttgct gaggtcgtgg tgaacgatga gaaccgtctt cccagctttt ttcaaatctc
    29221 tcagcgtatt catgatgatt tcctcactga cagagtcaat cccagcaaag ggttcatcca
    29281 agaggatata gtcggcttcc tgcaccaaac atctggcaat caagacccgc tggaattgac
    29341 ctccagacag ttgactaatt tgacgttcag cgtagtcagc taggccgacg atttcaaggg
    29401 cctcttgcac tttcttccaa tgtttagcct ttaaacttcg aaagagagga atagagggaa
    29461 atagtcctaa cgagacgcat tccttgacct tgatgggaaa gttgtagtcg atattgattt
    29521 tttgttcgac ataggcaatt cggtgtaagg attttttaac ttccttgtca tcgagaaatg
    29581 cctgaccttg atgtgggata attcccagca taccttttaa tagtgttgat ttcccagcgc
    29641 cgtttggacc aatgatgccg gtaattgttg gtccatggag cactagtgaa atatccttaa
    29701 gtgccaacgt ttctttgtag gagacactga ggttttcgat acgtatcata aacttgtatt
    29761 cctcctgtct cttaatatac attaaaaaaa aaattaagtc aagttaattt ttgaaaaaat
    29821 taaaataata actgaaaaat agattctaaa gataactttc aggataaatt tctaaattat
    29881 aaaacgcata gtatcaagtg taaaaaactt ggaattatgc gttttatcat ggaaagattt
    29941 tttataatag ctaaaaaata atagttaaat ggttattaat tgcattccct agtgattttt
    30001 gttaagataa atgcaaatac aaatgaaagc gagaacaaga tgacacgtta tcaagatgat
    30061 ttttatgatg ctatcaatgg agaatggcaa cagacagctg aaatcccagc agataagtct
    30121 caaacaggag gttttgttga tttagaccag gaaattgaag acctgatgct ggcgacaaca
    30181 gacaagtggt tagcaggtga agaagtgcct gaggatgcta tcttggaaaa ctttgtcaaa
    30241 taccaccgcc tagttcgtga ttttgacaag agagaagctg acggtatcac acctgtctta
    30301 ccactcctta aagaattcca agaattggaa acttttgcgg attttacagc taaactagca
    30361 gagtttgagc ttgcaggaaa accaaacttc cttccttttg gtgtatcgcc agactttatg
    30421 gatgctagaa tcaatgttct atgggctagc gctccaagca caatcttgcc agatacgacc
    30481 tactatgcag aagaacatcc tcagcgcgaa gagctcttga ctctttggaa agaaagcagc
    30541 gcaaatctcc tcaaggctta tgatttctct gatgaagaaa ttgaagactt gctagaaaaa
    30601 agacttgaat tggaccgccg agttgcggca gtggtgctct ctaatgaaga aagttcagaa
    30661 tatgctaaac tctatcatcc atattcttac gaagatttca agaaattcgc gcctgcccta
    30721 cctttggatg acttcttcaa agcagttatt gggcaattac cagacaaggt tattgtagac
    30781 gaggaacgtt tctggcaagc agcagagcaa ttttacagtg aggaagcctg gtctctcctt
    30841 aaagcaacct tgattttgag tgttgtcaat ctttcaacca gctatttaac agaggatatc
    30901 cgtgttttgt ctggtgccta cagccgtgcc ctttctggag ttccagaggc aaaagataag
    30961 gtcaaagcag cttatcatct agcacaggaa cctttcaagc aagccctggg gctttggtac
    31021 gcccgtgaga agttctctcc agaagccaag gcggatgtgg agaaaaaagt ggcaaccatg
    31081 attgatgtct ataaggagcg tctgcttaag aatgactggc tcactccaga aacctgtaaa
    31141 caggctatcg tgaagctcaa tgtgatcaaa ccttatattg gctatccaga agaattgcct
    31201 gcacgttaca aggataaggt agtgaatgaa actgccagtc tttttgagaa tgctctagcc
    31261 tttgcgcgtg tggaaatcaa gcacagttgg agtaagtgga accagcctgt agactataag
    31321 gaatggggca tgcctgctca tatggtcaat gcctactaca atcctcagaa gaacctgatt
    31381 gtctttccag cggccatttt acaggcgcct ttctatgact tgcatcagtc atcttctgct
    31441 aactacggtg gtattggggc agtgattgcc catgaaattt cccacgcctt tgatactaac
    31501 ggggcttcct ttgacgaaaa tggtagcctc aaggattggt ggacagagag cgactatgct
    31561 gccttcaagg agaaaacaca aaaagtcatt gaccaatttg atggacagga ttcttatgga
    31621 gcaaccatta acggtaaatt gactgtatca gaaaacgtgg ctgacttggg aggaatcgca
    31681 gcagcgcttg aagcagctaa gagagaagca gacttctcag cagaagagtt cttctacaac
    31741 ttcggtcgca tctggcgcat gaaaggtcga ccagaattta tgaaactttt ggctagcgtc
    31801 gatgtgcacg caccagccaa actccgtgtc aatgtgcaag taccaaactt cgacgatttc
    31861 tttacaacct atgatgtcaa agaaggagac ggaatgtggc gttcaccaga ggagcgcgtg
    31921 attatttggt aatacaaaaa atctagtcat cagaaaaaag gcatccaatc acgtgtgaaa
    31981 accttgatag gatgcctttt gtaatagaaa gatttgctgg atagtttact taaatttcga
    32041 ttgtcatcat cttgctagaa aaagggattg aaggcctttt cgtgagcgat agtagtagct
    32101 ggaccatgtc ctggatagac atcgtagttt ggtagggtga agagttgggt ctggatacta
    32161 tgaaggagtt gctccatgct accagtcgga aggtcggtcc gtccgatagt ttcgcggaat
    32221 agagcatctc ccgtcaagac taggtgagca tcaggaaaga ctagggaaac accaccgata
    32281 gagtgccctg gggtcggtag aaccttaaaa cgaaattcct ctagttggta ttcttcgtga
    32341 aagacaaagg tgtgttctgc aggttttgtg accacatctg ccatatcatc gtggcgaggg
    32401 agaccggaga gattatcgac aggagtgtag agccagctgg cttcgctctc tgcgatatag
    32461 acaggaggat tgccaaacgt ctcgcgaacc aagtccagac tcatgatatg gtcataatgg
    32521 gcgtgggtca agagaatagc acagatcggt ttgttgatct tctcgattgt ctgacgaatg
    32581 gcttcccaat gactaccagg atcgacgacg atgaggtgct tttcgccttc tagataatag
    32641 gtattttcat aggcaacagg attcacggtt ttatggattt tcatactagc tccaatctca
    32701 aagaatgtac taatactctt cgaaaatctc ttcaaactgc gtcaacttta tctgcaacct
    32761 caaagcagtg ctttgaacag cctgcggcta gctttctagt ttgctctttg attttcattg
    32821 agtataaatt aagtatagca cagttaggga gaataggtaa ggatttaaaa tgaacttaaa
    32881 aaccagcatg actagatgaa gacaggctgg ttatcaattt atcaatattg gaaaggtgtc
    32941 aattgccctt cataggttgc ataaactccg tcattagctt gtttgataga cgtgatgttt
    33001 agagtgctac cgtagttggc ttctcgtttg tcgctatatt cactataagt tatcctactg
    33061 ggcaggttct ggtcgttagc atagtattga acgactgttt tttatagctt tgctggctga
    33121 ataggaacag gcagcaaact cctaacatta ggagagcaaa gatagaaata gcagtttttt
    33181 tcataggtat tctccttata aactaaagga tagaagaaag tatcttgagg atttttatac
    33241 tcttcgaaaa tctcttcaaa ccgcgtcaac gtcgccttgc cgtatatatg tgactgactt
    33301 tgtcagtctt atctacaacc tcaaagcagt gctttgagca acctgcggct agtttcctag
    33361 tttgctcttt gattttcatt gagtattaat tccaaaatcc agtcaaggta ccagtgtatg
    33421 tgatacgagt tccaccgttt atctttcgta tcttcaatgg aattggggaa atcagaaagt
    33481 ctttgggtta caatatggtt tccgttcttc tatctctcct tctatcacct aagtttaaaa
    33541 tctaacttcg tagtatatta tgggccaata attcagaatt atcaagcaat tttgaattat
    33601 ttttttctta ttctgttcca atctagtttt catattctta aaaaagtgat aaaatggtag
    33661 gaagaattgg agagtagaga tgccgaaaga agtgaattta acaggcgaag aagttgtcgc
    33721 tttaaccaaa gaatatttaa cggaagagga tgttcatttt gtccataagg ccttggtcta
    33781 tgctgttgaa tgccacagtg gtcaatatcg caaatcaggc gagccttata tcattcaccc
    33841 tatccaagtg gcaggtattt tagctaagct aaagctggat gctgtaacag tagcttgtgg
    33901 attcttgcat gatgtggtgg aagatacaga tgcgaccttg gacgatttgg aaagagagtt
    33961 tggtcctgat gtgcggatga ttgttgacgg agttaccaag cttggcaagg tcgagtacaa
    34021 atcgatcgag gagcaattag cggaaaatca tcgcaagatg ctcatggcca tgtctgagga
    34081 catccgcgtt attttggtca aactgtctga ccgcttgcac aatatgcgga ccctgaaaca
    34141 tcttcgaaaa gacaagcagg agcgtatttc caaagaaacc atggaaatct atgccccgct
    34201 tgcccatcgt ttggggattt ccagtgtcaa atgggaatta gaagacttgt ctttccgtta
    34261 tctcaatcca acggagtttt acaagattac ccatatgatg aaggaaaagc gcagggagcg
    34321 tgaggccttg gtggatgagg tagtcacaaa attagaggag tatacgacag aacgtcactt
    34381 gaaagggaag atttatggtc gtcccaagca tatttactca attttccgca aaatgcagga
    34441 caagagaaaa cggtttgagg aaatctatga tctgattgct attcgttgta ttttagatac
    34501 ccaaagtgat gtttatgcca tgcttggtta cgtgcatgaa ttttggaaac cgatgccagg
    34561 tcgcttcaaa gactatatcg ccaaccgcaa ggccaatggt tatcagtcta tccatacgac
    34621 tgtttatgga ccaaaagggc cgattgaatt ccagattcga accaaggaaa tgcacgaggt
    34681 ggctgagtac ggggttgcgg ctcactgggc ttataagaaa ggtataaagg ggcaagttaa
    34741 cagcaaggaa tcagctattg gaatgaactg gatcaaggag atgatggagc tccaagacca
    34801 ggctgatgat gctaaggaat ttgtggactc tgttaaggaa aactatttgg ctgaggagat
    34861 ttacgttttt accccagatg gagctgtccg ttcccttccc aaagattcag gaccgattga
    34921 ttttgcctac gaaatccata ccaaggtcgg tgaaaaagca actggtgcca aggtcaatgg
    34981 ccgcatggtt ccactgacaa ccaagttaaa gacaggggat caggttgaaa ttatcgccaa
    35041 cccgaactcc tttggaccta gccgtgactg gctcaatatg gtcaagacta gcaaggcgcg
    35101 caataagatt cgccagttct ttaaaaacca agataaggaa ttgtctgtca acaagggtcg
    35161 tgagatgctg atggctcagt tccaagaaaa tggctatgtg gcaaataaat ttatggacaa
    35221 gcgccacatg gatcaagttc tgcaaaagac cagttacaag acagaagact ccctctttgc
    35281 ggccattggt tttggggaaa tcggtgcgat taccgtcttt aaccgtctga ctgaaaagga
    35341 acgccgtgag gaagagcgtg ccaaggccaa agctgaggca gaggagcttg tcaaaggtgg
    35401 cgaggtcaag gttgaaaata aagaaaccct caaggtcaag catgaggggg gagtggttat
    35461 tgaaggtgct tctggtctcc tagtgcggat tgctaagtgt tgtaaccccg tgcctggtga
    35521 cgatattgtt ggctacatta ccaagggtcg tggtgtggct attcaccgtg tggactgtat
    35581 gaacctgcgt gcccaagaaa actacgagca acgtctcctt gatgtggaat gggaagacca
    35641 gtactctagc tcaaataagg agtatatggc ccatatcgat atctacggtc tcaaccgtac
    35701 aggactgttg aacgatgtac tgcaagttct ttcaaataca accaagaata tttcaacggt
    35761 caatgcccaa ccaaccaagg atatgaagtt tgctaatatc catgtgtcct tcggtattgc
    35821 caacctctct acactgacca cggttgtcga taaaattaag agtgtgccag aagtttactc
    35881 tgtcaaacgg accaacggct agttgtccta gctcttacta gaaaggctat tatgaaaatc
    35941 attatccaac gggttaaaaa agcccaagtg agtatagaag gccagattca gggaaaaatc
    36001 aatcagggat ttttattgct ggttggtgtt ggaccagagg accaagagga agatttggac
    36061 tatgctgtga gaaaactggt caatatgcgg attttttcag acgcagaagg caagatgaac
    36121 ctgtctgtca aagatattga aggagaaatc ctctctattt ctcagtttac cctctttgcg
    36181 gatactaaga aaggcaatcg tccagccttt acaggggcag ctaaacctga tatggcatca
    36241 gacttctatg atgctttcaa tcaaaaatta gcgcaagaag tgcccgttca gacaggcatc
    36301 tttggagcag atatgcaggt tgagctggtt aataacggac ctgttaccat tatcctagat
    36361 actaaaaaga gatgagaaag accaagccca gtcggcttgg tctttctcat ctatcataaa
    36421 atactccaaa aagaaatcgg ttcttgatat gcttggggga agtctctttt caggctttgg
    36481 cagatgcgat aggaagggat gagatgtcct aggatgagga gagttccctg aaggaaaagt
    36541 ggaatatcac ttaaagacac caaggagaga atcatcaggc tatcccatag gagaatttgt
    36601 aaagcaaaac gccagaatct cggtctaatc tgggaataga gttaactaaa gtggtcacaa
    36661 agttggtttg aaagataggt caatagcaca ggatgaaaga aaatgagcca accgctataa
    36721 atcaaaatcc agtcccaagt aagctccctt ttaaatgtca aaaatgccag catgattcca
    36781 tcaatgacaa ggagttgaat ggttttgatg aatatgattt ttttcatgct aaaacctcct
    36841 tttccttaga gacactccca taaagagatg tgtttgtcgt aaaagtctgg atagttttct
    36901 cctaggtagt cagctgtcat ataataaaaa gtagaatggc gagcagtgat gattggcata
    36961 agagatgaaa aacgctgaga actaatgaag gctaagagaa caaaaaatag aaaatcaatg
    37021 gtgagaatcc ctaaatagta gaagcgaatc tttttattga tttgaggata aatctcagcg
    37081 aattgatgtt tgagtcggac gaccaagaga aatagcagga gtccttgatc aaggaagaaa
    37141 attaaaccag agagcaagag ccactcaaaa gatgtctcga gtctagtgat aaaaaatgca
    37201 aatagtagca aatcaataac tgcaattgct ataaaatgga ttctatagag ttttttcatg
    37261 acaagacctt cctcttttat ctaacttcat tctactccaa aagaatggga gttacaacta
    37321 aaatgataaa aatagcagag gggagattct cttaagttgg ctagtattct ttatttgagt
    37381 ttccttctat tatctaactt cttcatcatt ccagacaaat aaagctccga ttgcattgag
    37441 gatataaaag atgtatttac cgatattggc gaagtttcct tgaataccag cttttgtcag
    37501 ctgaacgaaa ttgtaaatca accaaaagcc ccactgagtt gttagtttca atgcattcaa
    37561 agcattggca atgagggaca gtgcaaaggc aatagttgtt acgtaggcaa ggagattcat
    37621 cttgccccca tatccgatat agttggtcac aaaggcaaag aggaaggcga tgatggaaat
    37681 gatgatgggc gccaatttta cctgtttttg gctcatttgg ttgggtctgc cttcttgcga
    37741 agctttccat ttctttatag caaaggtata aatgaggaag gtgacgggat aggtaatgat
    37801 ggccgcctta tttccaagga tataatcaat agcaccggac aaaatggtat taacaatacc
    37861 aaagtaattt ccccatttgc ttaatttccc cgtgaaacga gtggacaaca tggaaatccc
    37921 aacgttagtt acggaaatca atccaaaggg tacaagagct gtccatagtc cccagtctac
    37981 aaatttatgg aggcgtgagt tgagataacc agatgcaatc gcaatcccaa cgaccaaagc
    38041 aaccccgaag aggtcaaact atttagatgt agcaaaaatt tttagtgatt ttttcatagg
    38101 ttaaactacc tttctttttt tcaaatattc tcccattttt ttcaaatatt ctcacaccaa
    38161 atgaaagtaa aataaaatga tagaaataaa accctgaaaa taaaactttc agggttggta
    38221 ggggacttga gaaattagtt gattttctcg acatagagtt gttttgcaat gtccatattc
    38281 acttgtaaat cctcgtcgtt actgaggata gtgaaggtat tgctgaagcc atcaaactgc
    38341 ttgacttgga gctggtcacc gatgtgaagt gagtgcttgt ccagataatg gagaatgtca
    38401 aaactatcgt gcacccgagt caggcggtag gcgccagctt ccttgatatc agctagtggg
    38461 aggttattga tttcaacgag gagttctccc ttggcaggaa tagttcctcc gtgggggcag
    38521 gttttaggga aacctagcag tttatctagt ctttccacga acaggtcaga gacagtgtgt
    38581 tccaagacct cagcttcctc gtgaatctgg tcacttgtat agtctaaatg atgaactaga
    38641 aaaacttcaa tcaagcggtg cttacgatag agctcagaga ccagtttgag accgaggtca
    38701 gtcagtagat agccacattc cttgtccttt aggatgagat tttcactttt cattcgtttg
    38761 atcatttcag ttacggcagg gggagagact tgcatgcgag ccgcaatttc cttgttggta
    38821 atcttatgca ggtctatgcc aatttcataa atacatttta gatagtcttc tttgtttggg
    38881 gtcattcgtt tcctcttgtc taatatcttt atctagtata tcaaaaaaag ctagatttct
    38941 ctagctgtga ctttttatgt tatacttatt gcaagagaaa aaacgaggag atggatatga
    39001 cgaaaatagc tcttctttca gatattcatg gaaataccac cgccttggag gctgttttgg
    39061 cagatgctcg gcagatagga gtggatgaat actggctttt gggagatatt ctcatgccag
    39121 ggacagggcg tagaaggatt ttggacttgt tggatcaact accgattacg gctagagttt
    39181 tgggaaactg ggaagacagt ctttggcatg gtgtccgtaa ggaattggat agtactcgcc
    39241 ccagtcaacg ctatctcttg cgccagtgcc agtatgtttt agaggaaatt tccctagaag
    39301 aaattgaagt gctccacaat caacctcttc aaattcatcg tcagtttggg gatttgacgg
    39361 tgggaattag tcatcatctg cctgataaga actgggggcg agagttgatt catactggca
    39421 aacaagagga ttttgaccgc ttggtgactc atcctccctg tgatattgct gtttatggac
    39481 atattcacca gcagttgctt cgttacggga ctggtgggca attgattgtc aatccgggtt
    39541 cgattggcca acctttcttt ttggacgctc agctacggaa ggacttgcgg gcccagtata
    39601 tgattttgga gtttgatgac aagggcctgg tagatatgga cttccgacgg gtagactacg
    39661 atgtggcagc tgaattgcag ctggctaaag acctgagact tccctatttt gaggtttact
    39721 atgaaagtct ggtcaatggg atccaccata ctcatcatca ggaatttttg agagaattgg
    39781 ctcagaaaga gggctgcgac cgggagttag acgactggtt gaaaagtggt aacgattgac
    39841 attacaacta aaaagggtat aataatagaa aaagaagagt agaggcaggg tagcctcgta
    39901 aaaaggagaa gaggatgcaa attccaagta gatttaccat tgcgactcat atgctgataa
    39961 tcattgccct cgaggggaag gaaagcaagg tgaccagtga ttttctggct gctagtgtcg
    40021 gggtcaatcc tgtcattatc agaaagatct tgtcccagtt gaagaaggca gagctgattt
    40081 cagtagcgcg tggaacgggc ggaacagaga ttgtcaagga ccttaaggat attagtcttt
    40141 tagatgttta tcaggcggtc gaatgtcttg gtaagacagg tcaactcttc agtttccatg
    40201 acaatccgaa tccaaattgc cctgtaggag ctcatattca tgatgttttg gatcaaaaat
    40261 tggagagaat tcagttgact atggaggcag aacttggtca aaccagtcta gaaaaagtcg
    40321 tggccgatgc agagagtcag atgaaggatt aagagggata aatgccctct tttttctttg
    40381 gataattatg ataaaatgta gctattaaag gagtaagaaa tgtatctcgt tataggaatt
    40441 gtattagcct ttatagtgtc attttggaag gataatagaa gtttgtggaa tccagtatta
    40501 tttttattat ccctcatttc aagttatttt tatctatcct acctttttta taaaaatgga
    40561 tacgagaatg ttcagttagc tttttatgta tttgcctttg tcttacttcc ttttttgctt
    40621 tttctaagtg gcatcttttt aatctataat ggggtcattt tgttgaagcg agagggacgt
    40681 tccaagcccc attatctctc aatgctcttt gatttttatt gactattaga ttctatttaa
    40741 aaagacttag aatgcacatg aaagtatgta ttctaggtct tttgttttgt atacatttaa
    40801 ttttcaagag tattatctat ttttccatgc ctggtaacga gtatcaatca ataactgggt
    40861 aaggcgaccc aaggtttctc tttcttctgg agtgagggat tgagcttgga caaagagatg
    40921 atgaatgtat tcaagcgcct ttaaggaagc caagtcattg atagagctaa taccagcctc
    40981 aagaatagtg ccaaagaaga ggtcgaagta gagctgaagt tcttcgtcag ggactgtggc
    41041 cacccattct ttaaaggttg tgtctacttg ctggctatca ctgttggtct tatccagttg
    41101 gacgaagtgc ttgtcctcaa tctgccaact aaaggtatcg tgctgggcga tgccacccag
    41161 ggcagtactc tgaacgatga tttggtgagc aggaatttcc agcatcatac cgataatgga
    41221 accttgtgga atgaatatct tgcttctatc cattatcctt tgataacccg cagtctgtgt
    41281 caattcttga tggagaccag gtgcatcaaa tgtataaact gctgtgatct gattttgcaa
    41341 actttgctca atttggctag cagcatagat agcgagattt cctcccttgg aatgcccagc
    41401 tagaataacc ttttgcttag gatgatgggc aaaaaagttc tttaaatagc gaagggcgtg
    41461 cttttgagca ggaatttcct tcatataggt caggtggaaa tcttccttcc agccaatgat
    41521 actgtcatct gtcccacgaa agacaatcag ataggtatcg aggctgacac gataagtcat
    41581 agccgcaaat tgcttttgca gttcagggtc gatgtcgttg ataaaatggg agagtttgca
    41641 atttttgaag cgcttgtgtt gagccaattc atctaataat tgaaggcgat ttttgctagt
    41701 aagcatggtg ggatctcttg gaacctgagg tgctagatct aaaagacgtt gaggaagtgt
    41761 ggagaccaga ttatcaaagg agaggtaggt gatttctatt aaggttagaa tgtctaactc
    41821 attcaaggga aggtcgtaat aagaatcata tgcgacatct ttcagatagt caaaaatatt
    41881 ggccataaga gctcctttct ttttatttat cttagcaaat ttccctcaaa ttagctagta
    41941 gcattgcctg tttgtactgg ctaaaaacag gctatttcaa attcagtttc agaccatctg
    42001 gcatggaaaa atctgttata ataatggaaa aggagaagcg catgcacaag attttattag
    42061 tggaagatga tcaggtcatt cgtcaacagg tcgggaaaat gctctctgaa tggggatttg
    42121 aagtggtcct ggtagaagac tttatggaag ttttgagtct atttgttcag tcggaacctc
    42181 atctggtcct catggatatt ggtttgccct tgtttaatgg ttatcactgg tgtcaggaaa
    42241 tccgcaagat ttccaaggta cctatcatgt ttctttcttc gagagaccag gctatggata
    42301 ttgtcatggc aatcaatatg ggggcggatg actttgtgac caagcctttt gaccagcagg
    42361 ttcttttagc taaggttcag ggcttgttgc gtcgttccta tgagtttggg cgtgatgaga
    42421 gtttgctgga atatgctggt gttatcctca ataccaaatc catggattta cattatcaag
    42481 ggcaagtctt gaatttgacc aagaatgaat tccagatttt acgcgtgtta tttgagcatg
    42541 caggcaacat cgtagcacgt gacgacctga tgcgggaact ttggaacagt gactttttca
    42601 ttgatgataa taccctctct gtcaatgtgg ctcgtttgcg taaaaagttg gaggagcagg
    42661 gattggtagg atttatcgag accaagaaag gaatagggta cggattgaag catgcttgat
    42721 tggaaacaat tttttctagc ctatctgcgc tcccgtagtc gtctttttat ctatctgctt
    42781 tctttggcat ttctggtctt actctttcag tttttatttg ccagtctagg aatttacttc
    42841 ctctactttt tcttcttgtg ttgctttgta accatcttat ttttcacttg ggatatattg
    42901 gtggaaacgc aggtctatcg ccaggaactt ctctatggag agagggaagc caagtctcct
    42961 ttggaaatag ctttagcaga aaaattagaa gcgcgtgaga tggaactcta tcagcagagg
    43021 tcaaaagcag aaagaaaact gacggatttg ctggattact ataccttgtg ggtccatcag
    43081 ataaagaccc ccattgcagc cagtcaactc ttagttgcag aagtggtcga ccgccaactg
    43141 aagcagcaac tagaacagga aattttcaaa atcgactcct ataccaacct agttttacag
    43201 tacctgcgtt tagaaagttt ccatgatgat ttggtcttaa agcaggttca aattgaggac
    43261 ttggtcaagg aaataattcg taaatatgct cttgtcttta ttcaaaaagg cttaaatgtc
    43321 aatctacatg accttgataa agaaatcgtg acggataaaa agtggctgct agtggttatt
    43381 gagcaaatca tctcaaacag tctcaagtac accaaggaag gtggtctgga gatttatatg
    43441 gatgaccaag agctttgtat caaagatacg ggaatcggga taaaaaacag tgatgtcctc
    43501 cgagtatttg aacgtggctt ttcaggatac aatggccgtt tgacccagca gtcctctgga
    43561 cttggccttt atctatctaa gaaaatttct gaagaactgg ggcaccagat tcgtatcgag
    43621 tctgaggtcg gaaaaggaac gacagtgcgg attcagtttg ctcaagtgaa cttagtcctt
    43681 gagtaaaaat agatgagaaa aagcctccag attggaggct tttatgctag actcttattt
    43741 ctcaacgcgt gatttgttgg cgatatcagc taggatagct tgaacaaaat tatcaactga
    43801 gacagtttgt gtttcttttt ggccgtagcg acgaacgttg actgtttcgt cttccatttc
    43861 tttgtctcca acaattaatt ggtaaggaat cttgctggtt tgtgaagcac gaatcttgaa
    43921 ctgcattttt tcattgcgct catctacgtc tgcacggaca ccgcggtcac ggagtttctt
    43981 ggccacttcc caagcgtagt ccacgtgttt ttcgttagat actgggatga gggttacttg
    44041 gtgtggtgcc agccatgttg ggaaggcccc cttgtagttc tcaatcaaga tagctgtgaa
    44101 gcgttccata gttgagataa ccccacggtg gatcatgact ggacggtgat cttcgccatc
    44161 agctccgatg tatttgaggt cgaagcgttc tggcaacaag aaatcaagtt ggatagtaga
    44221 aagggtttct tcttttccaa gggcagtttt aatctggata tccaattttg gtccgtagaa
    44281 ggctgcttca ccttcggctt caaagtagtt cacgcccatt tcatcaagag ctgcacgaag
    44341 catggtttgg gcattttccc acatctcatc gttatcaaag tacttatgag tatcttgagg
    44401 gtcacgaaga gagaggcgga agcggtagtc agtcaagttg aagtcttcat aaacatcgat
    44461 aatcaactga agggcacgtt ggaattcttc ttggatttgt tctggagtaa cgaataggtg
    44521 accgtcgttg agtgacattt cacgtacacg ttgaaggcca gtgagggcac cagatttttc
    44581 gtaacggtgc atcataccga tttcagcgat acggattggc aattcacggt aagagtgaac
    44641 atggtgtttg aaaacttgga tgtggtgcgg acagttcatt ggacgaagga caaattcttc
    44701 cccgtcaccc atgtccatgg ttgggaacat gtcttcttgg taatgatccc agtgaccaga
    44761 agtcttgtaa agctcaacag aagcaagtgg tggagtgtag acgtgttggt agccagaagc
    44821 caactctttg tttacgatgt agcgttccaa ttcacgacgg atagtcgcac catttggcaa
    44881 ccagaatggc aaaccttgtc ccacttcttg tgaaattata aagaggtcaa gctctttacc
    44941 aagtttacgg tggtcacgtt ccttagcttc ttcacgcatt tgaaggtagt ttttcaagtc
    45001 tttcttgtca aaccaagctg taccgtagat acgttgcatc atagcgttgt cgctgtttcc
    45061 acgccagtac gcaccagcta catggagaag gtggaagatt tggatacgac ctgttgatgg
    45121 aacgtgaggt ccacggcaga ggtctacata ttcaccctga cgatagatag tcaaaccgcc
    45181 ttcgtcttct gagtgttctt caatcaattc caacttgtaa gggtcatttt tgaagatttc
    45241 acgtgcctcg tctttagtca cttcttcacg aatagatggg aagttttctt tgacgatttt
    45301 ttgcatttct tcttcgatac gaggaaggtc ttcgttagag atttgaccag ctgtgttgtc
    45361 agtatcgtag tagaaaccat cttcgatggc tggaccaact cccaagtgaa tgtctgggaa
    45421 aagacgacga gctgcttggg cgaacaagtg agctgctgag tgacgcaaga ttggaagggc
    45481 atcttcgtga tcaggtgtca caatttcgat gcttccatct tcagtgatag cgcgagtagt
    45541 gtcgatgagt ttgccgttga atttaccagc caaggctttt ttagctaggg aattgctgat
    45601 agattgggca atttcaaaag ttgttacgcc agattcgaat tcacgaacag cgccatctgg
    45661 gaaagtaatg ttaatcatga tgttctcctt ttttattatt tcatagtttc taagcattga
    45721 ttttttatca gcagactact tccaagtcct aacgttcaat tgcttttctt tatcagagag
    45781 gaagttttcc atttccgtca gaatgtctat atccaaacgt tgagaaagtt ctaatagcca
    45841 ccagatattt tctgaaagtt tttgttccag tgtgtagggt gtttcatcat agtagcgtcc
    45901 ttgctttgtc atcaccagtc gttggaaatt tccaatatca ttagataaag ccaagaggtc
    45961 ttcttctacc gtccacttgg aatcatgatg cttaacttcc agttcgtgat aagcttgtcg
    46021 gattgcccaa ctgcgctcca ctaattcttg caactccata attagtatcc taataaaaaa
    46081 atgcgacatc ctcgaaagga cgtcgcaacg tggttccacc ttcatttatg tacctcaaaa
    46141 gagagggaca cctctgattg gctctaacgt ggccaccgtt ttatgttttc ataaaaaact
    46201 caagagtagt atcagtttag acttcctatg cgtttccagc aaccacgcac tctctagtag
    46261 aaagggacta agtgacttgt ctctatggat tattatagca tgattgtgag atttttcaag
    46321 gattttgtga aagttttttg tttttcatat tttgtagaat atgcttgatg ctaataccag
    46381 aatccatgcc caccagagca actgtttcat agattagcaa gtaaattagt ataaactcac
    46441 caaaggttat gatagtaaag taaaagagca gtccgttacc aactaaccat caaagtgaga
    46501 aacggaaacg gtagctatag agtaacgata caatcaagat taccagaggt gtaaagaaga
    46561 gaagattgtt tatatagtgt aagggggtca gaaacaacta gcaagaacaa ctcataaagt
    46621 ggccaataaa agaataatat gaccagatag taggggagat gggaatagaa tctacgcatg
    46681 ataatcacct cgttttaaaa aaacagacaa cttagatctc ttgctatgat tattatagca
    46741 tgtttaaaac aagaaagtaa atttgttgac aaagaagatt tttcttttca ctagaatcat
    46801 ggttagtttt cttggtagaa atattttgat aataattaag caagactatt gacattgatt
    46861 attaagagtg tcacaattgt tgtaactaaa acaattacaa caagagaggt ataaaaaatg
    46921 aaactagcag ttattgcagc aaatggacaa gcggggaagg caattgttga agaagcagtc
    46981 aaacgaggac atgaagtaac ggctattgtt cgttctgaaa acaaaagtca agctgagtct
    47041 attattaaaa aagatttatt tgagttgaca aaagatgatc ttactggatt tgatgctgtt
    47101 atttcagcgt ttggtgctta tacaccagat acgctaccgc tccacagcaa atcaatcgaa
    47161 ctatttaatc agcttcttgc tggaacacag acacgttttc tagttgtagg gggatcagga
    47221 agcttttata tagatgaaac aaaaacgaca cgacttttag atacgtctga ttttcctgaa
    47281 gaatttaagc cactagcaaa agctcaagcg gatgaattgg atcttcttcg tacaaaaaat
    47341 aatctcaact ggacttttgt cagtcctgct gtagatttta ttcccgatgg tgaaaaaaca
    47401 ggaaactaca ttctagcagg agaaattttt actactaatg aaaaaggaat aagccagatt
    47461 agttatgcag attatgctat tggattggta gatgaacttg aaaaaggtca tcatataaaa
    47521 gaacgtatct ctttacttga aaaataataa ctatgagagt attaatgttt gacagaaaat
    47581 ttaaatcttg gtaaaatagg gattgtcgta aagacaaata acttcttctt ggttacaggc
    47641 atgccaacct gtcactcgga tgaagccaaa taaaaaggag aaacatcatg gcaatctcaa
    47701 aagagaaaaa aaatgaaatc atcgcacaat atgcacgtca cgaaggtgat acaggttcag
    47761 tagaggttca agttgctgtc cttacttggg aaatcaacca ccttaatgaa cacatcaaac
    47821 aacacaaaaa agaccacgct acttaccgtg gattgatgaa aaaaatcggt cgccgtcgta
    47881 acttgcttgc atacttgcgt aaaaacgacg ttaaccgtta ccgtgagttg atcaactctc
    47941 taggacttcg tcgctaattc aagatacaaa ggctgtcaaa agcacaaagc aaaaatagga
    48001 aaattgacga agaaacttca gtttctagga gattttatct tttttgccaa gtgcttaggc
    48061 cgtgttcaat tgagcatatc ttgataatga agctactcta aattgggtag cttttttgcg
    48121 ttgtcacctt acctcgatat actcaagtat aatcttcggt tacggttcct agcactgtaa
    48181 ggtaaaataa accagctttt ctcccttcgg ggagattttt ttttgtttca ctgaaagttt
    48241 cgtacgatct tcgcctcgtc gcctagtcta taaacgattt atccagcaag aatcatgatg
    48301 ctaagggcgt caaaaatccg tatgaaaata gggaaatggc acagtgttcg atgaacgcaa
    48361 ggagtttcat ctttttcact aggattttag tccgggctca aatcagctct ctgacttcag
    48421 agggcttttt tattgaggtt ttatcggtaa caattttgga gactacaaaa acctcaaatg
    48481 gtatcagcta attacaccat aaaggtgcgc agttactcgg cttttcaagc cgagtagctg
    48541 tatgcaagcc ccctagaaga gcccactctt tacaaagtaa agtatagtat gttatacttt
    48601 acatggaagt agtcactgaa ttccagttag aaattacttt gtaactacgt tttgaggagg
    48661 agtaaaatgc tttcctacgt tcgacattac ccactagcga tagctaaatt aatgtgtctg
    48721 tgctctccta aaatctgctg atttattact gactaataca ggaggttttt ttatgggaca
    48781 gacaatcata tctgctattg gtgtttatat ttccaccagt atcgattatt taattatttt
    48841 aattatttta tttgcacaac tatcacagaa taaacagaaa tggcatattt atgcggggca
    48901 atatctaggc acaggcttac ttgtaggggc gagtttagtt gctgcttatg tcgttaattt
    48961 cgtgcctgaa gaatggatgg ttggattgct tggtttaatc cctatctatt tagggattcg
    49021 ctttgcaatt cttggagaag atgcggaaga agaagaggaa gaaattattg aaagattgga
    49081 acaaagcaag gcaaatcaac tgttttggac agttacattg ctgacaattg cgtctggcgg
    49141 agataattta ggtatctata taccttattt tgcttcgtta gattggtcac agaccctcgt
    49201 ggccttgctt gtgtttgtaa tcggcataat tatcttttgc gagattagtc gggtgttatc
    49261 ctctattccg ttaatattcg agacaattga aaaatacgag cgaatcattg tgcccttagt
    49321 attcattcta cttggactat acatcatgta tgaaaatggc acgatagaga cttttctgat
    49381 cgtgtagatt tttttgtttc actaggattt tagcccgagc tcaaatcagc tctctgattt
    49441 tcagagggct ttttcattga gtataaatgg acaatcttaa agttactcta ttgtagagtg
    49501 gcttttgatt tgattaggag attcttataa atatagtaaa atgaaacaaa aacaggacaa
    49561 atcgatcgtg acagtcaaat cgatttctaa aaatgtttta agaagtagag gtgtactatt
    49621 ctagtttcaa tttgatatat aaatgaaaac cttagctgtt tggccaaggt tcatttcttc
    49681 tatgcaagtt cttctctttt cttatctggg ttccagatga agcttgcgat aaagctaaag
    49741 atgatggtta ggatagcgag gataatagca aggatgaggg cagggatgcg gataaaccac
    49801 cagagtggat ttggtttttt atcattgtgc cattgtttcg tttcttcgtc cagacgttgg
    49861 aattctgttt gaatacgatt ggcgactgtt tcaatgcctt catcattcat tttcttgata
    49921 tctgagatat cgattggatt tccaaagttc atatcgacac gttcacggct aatcaagccc
    49981 ttcaaagtca tgggaccggt gtaggtaacc ggcatgatac ggaccttggc cattttggca
    50041 atcagtgctg cgcccccctt gacatcgttt gagtggcggc taccacttgg aaacatgatg
    50101 agagagcggt cacttttttt gagaacgttg ataggatatt tgatggctga ggcgctggga
    50161 ttttcacggt cgatgggaaa ggcgccacac atacgaatcc accaaccaaa gatacggttg
    50221 gtaaagagtt ctttttttgc cataaagatg aactgttttg gcttggtcgc aaaggccata
    50281 taaacaggat cccaccaggt acggtgaggc gcaactaaaa tataattttc atcttgatta
    50341 ggaattttat cagtattatg atagtgagca ttgccattga tggaccataa gagcaataca
    50401 actaatccac gcaaataagt ataaaacatg cgatctcctt cgattgtttt cttgttatta
    50461 ttatacctta tcaaaggagg gctggcaaac ttttcccttg actagatgca tatttgggat
    50521 gaaattagaa ttctgttaaa aaaaatgata taatagaatt tatggataaa aataagatta
    50581 tgggattaac ccaaagagaa gtcaaggaaa gacaggctga gggtttggtc aatgacttta
    50641 ccgcatcagc cagtaccagc acttggcaaa tcgttaaacg aaatgtcttt acccttttta
    50701 acgctttgaa ctttgccatt gctttggccc ttgcctttgt gcaggcttgg agcaatctgg
    50761 tcttctttgc tgttatctgc tttaacgctt tttctgggat tgtgaccgag ctacgagcca
    50821 aacacatggt ggacaagctc aatctcatga ccaaggaaaa ggtcaaaacc atccgtgatg
    50881 gtcaggaagt tgctcttaat cctgaagaat tagtgctagg agatgtcatt cgtttgtctg
    50941 caggagagca gattcctagt gatgccttgg ttttggaagg ctttgcggaa gtcaatgaag
    51001 ccatgttaac gggagaaagt gatttggtgc aaaaggaagt tgacggctta cttttgtcag
    51061 gaagtttcct agccagtggg tcagttttat ctcaagttca ccatgtcggt gcagacaact
    51121 atgctgccaa actcatgctt gaggctaaga ccgttaaacc catcaactcc cgtatcatga
    51181 aatcgctgga caagttggct ggttttactg ggaagattat cattcccttt ggtctggctc
    51241 tcttgctgga agccttgctt ttaaaaggcc tgcctctcaa gtcatccgtt gtaaactcgt
    51301 cgacagctct tttgggaatg ttgcctaagg gaattgccct tttgaccatt acttcgctct
    51361 tgactgcagt gattaagttg ggcttgaaaa aggtcttggt gcaggagatg tactctgttg
    51421 agaccttggc gcgcgtggat atgctctgtc tggacaagac gggcaccatc acccaaggaa
    51481 agatgcaggt ggaggctgtt cttccactga cggaaactta cggtgaagag gctattgcca
    51541 gcatcttgac tagctacatg gcccatagtg aggataagaa tccaactgcc caagccattc
    51601 gccagcgttt tgtgggagat gttgcttatc ctatgatttc caatcttccc ttctcgagcg
    51661 accgcaagtg gggggctatg gagttagaag gcttggggac agttttctta ggggcacctg
    51721 agatgttgct ggattctgaa gtcccagaag ccagggaggc cttggagaga ggatcacgtg
    51781 tcttggtctt agctctcagt caggagaaat tagaccatca caaaccacag aaaccatctg
    51841 atattcaggc tctagccttg ctggaaatct tggatcctat tcgagaggga gcggcagaga
    51901 cgctgaacta tctccgttct caggaagtgg ggctcaagat tatctctggt gacaatccag
    51961 ttacggtatc cagcattgcc cagaaggctg gttttgcgga ctatcacagc tatgtcgatt
    52021 gttcaaaaat cacggatgag gaattgattg ctctggcgga ggagacagcg attttcggac
    52081 gtgtttcccc tcatcaaaag aaactcatta tccaaacgct gaaaaaagcg ggtcatacaa
    52141 cagctatgac tggggatgga gttaatgata tcctggctct tcgtgaggcg gattgttcta
    52201 tcgtaatggc ggagggagac ccggcaaccc gtcagattgc caacctggtt ctcttgaact
    52261 cagactttaa tgatgttcct gagattcttt ttgaaggtcg tcgtgtggtc aataacattg
    52321 cccacatcgc tccgattttc ttgataaaga ccatctattc cttcctgtta gcagtcatct
    52381 gtattgccag tgctttacta ggtcggtcag agtggatttt gattttcccc ttcattccga
    52441 tccagattac catgattgac cagtttgtgg aaggtttccc accatttgtt ctgacttttg
    52501 agcgaaatat caaacctgtt gagccaaatt tcctcagaaa atccatgctt cgtgccctac
    52561 caagtgccct catggtcgtg tttagcgttc tttttgtgaa aatctttgga acgagtcaag
    52621 gttggtctga gttagaaatc tcaactctcc tctattatct cttggggtca attggtttct
    52681 tatccgtatt tagagcctgc atgccattca ctctatggcg agtcctcttg attgtctggt
    52741 cagtaggagg cttcctagcc acagctctct tcccaagaat ccaaaaactg cttgaaattt
    52801 caaccttgac agaacaaacg ttgcctgttt atggtgtcat gatgttggtc tttacagtga
    52861 ttttcatcct gactagtcgc tatcaagcta gaaaataaag aaagactgca atctgtggat
    52921 tgcagttttt tatacgatgg atctatcgta gatctgatgt gcaaggccta cgtgtctatt
    52981 atctatcggt gaacccaaga gtgattctca agcctgcttg gattgaggta atagattcaa
    53041 atatctgtag ttagactatt tgaagtttga tgtaagaaag agaaagcgac agattgaagt
    53101 aattttaact ctcttctatt gctagaacaa atggtcggat aggttggtag tttgaaaatg
    53161 aagatgcaat ctattgttaa atggaacata gtgttattta ttagaaaatc gtttggttta
    53221 tttcttatca aatacgaaaa gcaacttaaa tatttcaact aaaatagatg ttatgaagaa
    53281 aaggtaaaat gattttggta tagtgaggtt ctgttctatt tgatatcata tttttgataa
    53341 aaacaaaaat gtccattgca aaggacaaaa tgcgaagtat attatttttt gaaagcgata
    53401 taatggaatc ataaaggagg tgtatcgtgt ctagaaaaca agaacaaatg gaaacgttgt
    53461 tgctcctttt gcgagatagt aaggattata tatctgctaa agtattggga gaaaaattaa
    53521 attgctctga taaaacggtt tatcgccttg tcaaggtaat caacaaagat tgtccggtag
    53581 aagcattcat tttatctgaa aaaggcagag gtttcaaatt aaatccaaga agttccctcg
    53641 tggacgttga tgggaatttt acagaggctt ttgatcctga agtaaggcgt gaaaaattac
    53701 tagaacgtct cttgttgact gctcctaagc cacattctat ttatgattta ggagaggaat
    53761 tctacgtaag cgagtcagta gtactaaaag atcgtcagat attacaagag agtctagcaa
    53821 tttatgggtt agatttaaaa atgagacaac gaaagctttt tattgatggg gatgaggctc
    53881 aaattcgttc agccattcta aatctactgc caatgtttaa tcagttggat ttagagcaaa
    53941 ttacacagaa taaggttcag cctcttgacg gagaacttgc tcacttttgt ttgggattac
    54001 tgattacact tgagagagaa ttgggggtaa acattcccta tccatataat ataaatattt
    54061 tctctcacct gtatattttt atcagtagga atcgtcgtag tactagtatt catgttgtag
    54121 caccttcaaa acctactatt gttgatgaga aaatttacag tgtctgtcaa aaaattattc
    54181 aagaaattga acaatatttt aagatgaagg ttgatgcagt tgagattgac tatctttatc
    54241 aatacgttgt atcttcgaga ttgcaaaaac cattttcttc cgggaagctt cctttttctc
    54301 agcgagtttt agatgtcact cattactatt ttagccgtat gtgtatggac aatagagaga
    54361 ttgaaacgac agatcctgac tttgttgact tggcgagtca tatcagtccc ttactgagga
    54421 gattagataa tagagtacag attaagaata gtcttttatc acaaattctt ttaacctatc
    54481 ctaatctagt taaagagtta acaactattt ctaaagaagt gagtctagta tttggttttg
    54541 cgtccttgag tctggacgag attggttttc tagtcttata ttttgcacgg tttcaagaaa
    54601 agcgagcacg tcctctaaaa acagtagtga tgtgtacatc aggtgtcgga acttcagagc
    54661 ttttacgagc acgattagaa aagcaattta ctgaattgga tattattgat gtagttgctt
    54721 atcatcaatt agatgagctg ataaatctat atccagattt agatttcatt gtgacgacgg
    54781 tagctttgca ggaaccagca agtgtcccgt ttgtcctagt tagtgttttt ctaaccgagg
    54841 gtgataaaca acgtcttcaa gcaaaaattc aggagataaa ctatgaataa tctttcgctt
    54901 gtccttatgg atatatctgt tcaaaatcgt caagaagcct acaaagaatt agcaaatcaa
    54961 atcagccttc ttgtttctga agatacagaa aaaatagaag agcttctata ttaccgtgag
    55021 agacagggaa gtatagaggt tgctaaaggt gttcttctac cacattgtga aggaaacttt
    55081 caacatcatg tcttagtgat tactagatta aaatcaccta tcagagaatg gtcgaaggat
    55141 atccagtgtg ttgaccttat tatcggtttg gccattgcag tatcacagga caagtcatgt
    55201 attaaaacat tgatgagaag actagcagat gaatcattca taaatcaatt aaaacagtta
    55261 acaaaagaag aattacggga gataatatat ggaaatcaaa gatattctta atgtgagtct
    55321 gatccagacg gatttacaga tgcagagcaa agaagaggtt tttgaggcat tagctcaact
    55381 attggttgag acgggttatg tgtctgatag agaccaattt atcgaaggtc tgtatcagag
    55441 agaggcagaa ggacagaccg gtattgggaa ttatattgct attccccata gcaagagttc
    55501 tgctgtggag aaggcggggg tagtcatagc tataaatcac aatgagattc cttgggagac
    55561 cattgatggg aaaggggtca aagtaattgt actctttgca gttggtgatg atacagaagc
    55621 tgctagggag catttgaaga ccttatcact ctttgctcga aaacttggta atgacgaagt
    55681 tgttgccaaa ttagttcggg ctcagacatc tgatgatgtg attgcagctt tttgttaata
    55741 agaaaaaatt ttggagggta tccgtatgaa aattgttggt gttgcagctt gtactgtggg
    55801 aattgcccac acttatattg cacaggaaaa attagagaat gccgcaaagg tagctggaca
    55861 tgtgattcat gttgagactc aggggacaat aggggtagaa aatgaattga gtcaagagca
    55921 gattgatgca gcggatgtag ttattttagc agttgatgtt aagatttctg gtatggaacg
    55981 ctttgagggt aaaaagatta tcaaggttcc aacagaagtg gcagtcaaat ctcccaataa
    56041 actgattgct aaagctgttg agattgttac gaaataactg aaaatattta aggagaaaat
    56101 atatgttgaa acacttaaac ttaaaaggtc acttattgac agccatttcc tatatgattc
    56161 caattgtttg tggtgcagga ttcttagttg ccattggttt agcaatgggg ggtggtgttc
    56221 ctgacgctct tgtagcagga aaattcacta tctgggatgc tttagcaact atgggtggta
    56281 aagcccttgg tctcttgcca gttgttattg ctacaggttt gtcttactcg attgctggta
    56341 agccagggat tgcaccaggt tttgttgttg gtctaattgc caattctgtt ggttcagggt
    56401 ttatcggtgg tatcttggga ggttatatag ctggtttctt ggttcaagcg attattaaaa
    56461 aggtcaaagt accaaactgg attaaaggtt taatgccaac cttgattatt ccttttgtag
    56521 cctctttggt aagtagtttg attatgattt atattattgg agcgcctatc gcagccttta
    56581 ccaactggtt gacgagctta ttacaaagct tgggaagtgc ttcaaatggt ttgatggggg
    56641 cagttattgg agttctcagt gctgttgact ttggtggccc acttaataaa acagtctatg
    56701 cgtttgtgtt gactttacag gctgaaggtg tgaaagaacc attgactgct ttgcaattgg
    56761 tgaatactgc tacaccagtt ggatttggat tggcctattt tatcgcgaaa ttactcaaaa
    56821 aaaatatcta tactcaagag gaaatcgaaa cattgaaatc ggctgttcct atggggattg
    56881 tcaatattgt tgaaggtgta attccgattg ttatgaataa cttggttcca ggtctcattg
    56941 caacaggtat cggtggtgct gttggtggtg ctgtttcttt gacaatgggt gctgattctg
    57001 ccgtgccatt tggtggagtg cttatgttac caaccatgac tcgtccagta gctggtattt
    57061 gtgctttgtt agccaacatt gtagtcacag gacttgtcta cgcgattttg aaaaaaccaa
    57121 taaaacatac agaaccagtt atgactgttg aagaagagat tgatttgtca gatattgaaa
    57181 ttttgtaaga gggtaacgat gtcaagaatt gaattttcac catctttgat gaccatggat
    57241 ttggacaaat tcaaagagca gattactttt ttgaatgata aagtagcatc ttatcatatc
    57301 gatattatgg atggccattt tgttcccaat attaccttgt ctccttggtt cattcaagaa
    57361 gttcaaaaaa ttagtgacac acctttatca gttcatctga tggtcacaga cccaaccttt
    57421 tgggtagatc aagttctcga tttacaatgt gagtatattt gtattcatgc tgaagttctg
    57481 aatggtcttg cttttcgttt gattgataaa attcatgatg caggtctaaa ggctggtgtt
    57541 gtccttaatc ctgaaacacc tgtttctaca atctttccct acattgattt acttgacaaa
    57601 gtaactatta tgactgtaga tccaggtttt gcaggacaac gttttttgaa gtctaccttg
    57661 tataaaatcc aagaactccg tcagcttaga gttcagaatg gttatcacta catcattgag
    57721 atggatggtt cttcgagtcg taagactttc aaacaaattg atgtggcagg accagatatt
    57781 tatgttatag gtcgcagtgg attatttggt ttggatgacg atattgccaa agcctgggat
    57841 atctgttcta gagattacga agaaatgacc ggaaaaacaa tgccaatcaa ataatggttt
    57901 gagaagaaat ttattagtta ggaggaatat atgtcactac aatcagttaa cgccattcgt
    57961 tttcttggcg tagatgctat taacaaatct aattctggtc acccgggaat cgtcatgggt
    58021 gctgcgccaa tggcttatag cttatttaca aaacacctta gaattacacc tgagcagcca
    58081 aactggatta accgagatcg ctttatcttg tctgcgggtc atggatcaat gctactgtat
    58141 gctctcttgc atttaacagg gtataaggat gtatccatgg acgagattaa aaatttccgg
    58201 caatggggat ctaagacacc tggtcatcct gaagtgacgc atacgtctgg tgtggatgcg
    58261 acatctggtc cgcttggtca ggggatttct actgccgttg gtttcgccca agcagagcgt
    58321 tttttagctg ctaagtacaa caaagatggt tttcctattt ttgaccatta tacttatgtt
    58381 atcgctggag acggtgactt catggaagga gtgtctgcgg aggcggcttc ttatgcaggt
    58441 catcaagctt tagataagct tatcgtcctc tacgactcca acgatatctg cttggatggt
    58501 gagaccaaag atactttctc tgaaaatgtt cgcgcccgtt acgatgctta tggttggcat
    58561 acagttctgg tagaagatgg aacagattta gcagcaattt ctacagcaat tgagacggcc
    58621 aagttttctg gtaagccgag tttgattgaa gtgaaaacgg taattggtta cggctcaccc
    58681 aataaaagtg gtacaaatgc tgttcatggt gcaccactag gagcagaaga aacaggagca
    58741 actcgtaagt ttttgggatg ggattacgat ccatttgaag taccagagga agtatattct
    58801 gatttcaaga caaatgtagc ggatcgtggt caggaggcat acgatgcttg ggctagtttg
    58861 gtgtctgatt acaaggttgc ttatcccgaa gttgctagtg agattgacgc tattgtagct
    58921 ggaaaatccc ctgtaaccat tactgaaaaa gacttccctg tctatgagaa tggcttctct
    58981 caagcaactc gtaattcgtc ccaagatgct attaatacag cagcagcagt tttaccaacc
    59041 ttcttaggtg gatcggcaga cttagctcac tccaacatga cctacatcaa ggcagatggc
    59101 ttacaagata aatataatcc attaaaccgc aatattcagt ttggggtacg tgaatttgcc
    59161 atgggaacaa tcctcaatgg aatggctctt catggtggtt tacgagttta tggcggaacc
    59221 ttctttgttt tctctgacta cgtcaaagct gctattcggc tatcagccat tcaggagttg
    59281 cctgtaactt atgtctttac ccatgattca attgccgttg gtgaagatgg tccaactcat
    59341 gaaccagttg aacatttggc aggtttacgc tcaatgccaa acttgactgt tatccgtcca
    59401 gcggatgccc gtgaaactca agcggcttgg catcatgcct tgaccagtac caccactcca
    59461 actgtcattg tcttaacccg tcaaaacttg gtagttgaag aagggacaga ctttggtaag
    59521 gtcgctaaag gagcctacgt cgtgtatgat accccgggat ttgatactat tatcattgct
    59581 acaggatctg aggtcaatct agctatcaaa gctgctaagg aattggtttt acaaggtggt
    59641 aaagtacgtg tggtatctat gccctcaacc gaactatttg atgctcaaga tgctacctac
    59701 aaggaagaca ttttaccatc taagactcgt cgtcgtgtgg ccattgaaat ggcagcgacc
    59761 caaagttggt acaagtatgt tggtttggat ggcgcggtca tcggtattga catcttcggt
    59821 gcgtctgccc cagctcagac tgtgattgat aattatggat ttacggtaga gaatatcgtt
    59881 gctcaagtta agtccctata gaaaccaatt acaatgaaga tacagctgtt gtcagactag
    59941 cagatgtagt gatagacact aatcagatga ttggttattt aaaaactata atgagaatgt
    60001 aataatttat ctacgaaagt tatagtagat agtatacaca atagagtata ccctgaaacg
    60061 gttgcgaagt acgctaatca ctttgctact gatctagata gtttctttaa tcaataaaca
    60121 cagcatccac agattgactt aggatattgt aagttttttg aaagctagag agaaggtctc
    60181 taaaattaaa aaacgcatag tataggatgt tgaaatgatg aactgccccc caaaagttag
    60241 acagaaaaaa tctaactttt ggggtgtttt attatgaaat taagttatga agataaagtt
    60301 cagatctatg aactaagaaa gcaaggacaa agcttcaaac agctttcaaa aagatttggt
    60361 gtggatgttt ctggtctaaa gtacatggtg aaattaattg accgttatgg
//
LOCUS       DAVBNO010000006        59157 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVBNO010000006 DAVBNO010000000
VERSION     DAVBNO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 59157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59157
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.006"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            1..204
                     /locus_tag="VQ846_000348"
                     /pseudo
     CDS             1..204
                     /locus_tag="VQ846_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(321..668)
                     /locus_tag="VQ846_000349"
     CDS             complement(321..668)
                     /locus_tag="VQ846_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET3074998.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(779..2554)
                     /gene="spxB"
                     /locus_tag="VQ846_000350"
     CDS             complement(779..2554)
                     /gene="spxB"
                     /locus_tag="VQ846_000350"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET3074999.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2761..5004)
                     /locus_tag="VQ846_000351"
     CDS             complement(2761..5004)
                     /locus_tag="VQ846_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET3075000.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLVISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(5014..5385)
                     /locus_tag="VQ846_000352"
     CDS             complement(5014..5385)
                     /locus_tag="VQ846_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET3075001.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QVGEFGFACGMNMMHGKMIVE"
     gene            complement(5396..5791)
                     /locus_tag="VQ846_000353"
     CDS             complement(5396..5791)
                     /locus_tag="VQ846_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET3075002.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5975..6073
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6157..6948
                     /gene="thiD"
                     /locus_tag="VQ846_000354"
     CDS             6157..6948
                     /gene="thiD"
                     /locus_tag="VQ846_000354"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET3075003.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEGMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7157..7789)
                     /locus_tag="VQ846_000355"
     CDS             complement(7157..7789)
                     /locus_tag="VQ846_000355"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET3075004.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7782..8585)
                     /locus_tag="VQ846_000356"
     CDS             complement(7782..8585)
                     /locus_tag="VQ846_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET3075005.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8585..9109)
                     /gene="thiW"
                     /locus_tag="VQ846_000357"
     CDS             complement(8585..9109)
                     /gene="thiW"
                     /locus_tag="VQ846_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET3075006.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERIE"
     gene            complement(9114..9806)
                     /gene="tenA"
                     /locus_tag="VQ846_000358"
     CDS             complement(9114..9806)
                     /gene="tenA"
                     /locus_tag="VQ846_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET3075007.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9817..10467)
                     /locus_tag="VQ846_000359"
     CDS             complement(9817..10467)
                     /locus_tag="VQ846_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET3075008.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10469..11854)
                     /locus_tag="VQ846_000360"
     CDS             complement(10469..11854)
                     /locus_tag="VQ846_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075009.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILLQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREINQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11855..12415)
                     /locus_tag="VQ846_000361"
     CDS             complement(11855..12415)
                     /locus_tag="VQ846_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET3075010.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(12677..12765)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(12974..13603)
                     /locus_tag="VQ846_000362"
     CDS             complement(12974..13603)
                     /locus_tag="VQ846_000362"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET3075011.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(13605..14387)
                     /gene="thiM"
                     /locus_tag="VQ846_000363"
     CDS             complement(13605..14387)
                     /gene="thiM"
                     /locus_tag="VQ846_000363"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET3075012.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKKFKRVK"
     gene            complement(14798..15337)
                     /locus_tag="VQ846_000364"
     CDS             complement(14798..15337)
                     /locus_tag="VQ846_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET3075013.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENNYLEVTLHPVTKAFQDLMYWAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAE"
     regulatory      complement(15552..15640)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15894..17030)
                     /gene="lctO"
                     /locus_tag="VQ846_000365"
     CDS             complement(15894..17030)
                     /gene="lctO"
                     /locus_tag="VQ846_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET3075014.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(17656..19146)
                     /gene="lysS"
                     /locus_tag="VQ846_000366"
     CDS             complement(17656..19146)
                     /gene="lysS"
                     /locus_tag="VQ846_000366"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET3075015.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLKEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19274..19420)
                     /locus_tag="VQ846_000367"
     CDS             complement(19274..19420)
                     /locus_tag="VQ846_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HET3075016.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TSFQ"
     gene            19647..20315
                     /locus_tag="VQ846_000368"
     CDS             19647..20315
                     /locus_tag="VQ846_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET3075017.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRIPV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPLIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVVLPSFSANVIFLIKKTSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTCHAGFGNPST
                     LSRK"
     gene            20275..20655
                     /locus_tag="VQ846_000369"
     CDS             20275..20655
                     /locus_tag="VQ846_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET3075018.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            20665..20952
                     /locus_tag="VQ846_000370"
     CDS             20665..20952
                     /locus_tag="VQ846_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET3075019.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            20953..21716
                     /locus_tag="VQ846_000371"
                     /pseudo
     CDS             20953..21716
                     /locus_tag="VQ846_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21728..22594
                     /locus_tag="VQ846_000372"
     CDS             21728..22594
                     /locus_tag="VQ846_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HET3075020.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHLTYGDAAK
                     ADDLVVEGGKVD"
     gene            complement(22681..23328)
                     /locus_tag="VQ846_000373"
     CDS             complement(22681..23328)
                     /locus_tag="VQ846_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075021.1"
                     /translation="MNEIITLKNIQLELKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(23325..24023)
                     /locus_tag="VQ846_000374"
     CDS             complement(23325..24023)
                     /locus_tag="VQ846_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075022.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYVLG
                     ANEQMNFSCVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            complement(24033..24785)
                     /locus_tag="VQ846_000375"
     CDS             complement(24033..24785)
                     /locus_tag="VQ846_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075023.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(24785..25549)
                     /locus_tag="VQ846_000376"
     CDS             complement(24785..25549)
                     /locus_tag="VQ846_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075024.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(25645..26048)
                     /locus_tag="VQ846_000377"
                     /pseudo
     CDS             complement(25645..26048)
                     /locus_tag="VQ846_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26228..26860)
                     /locus_tag="VQ846_000378"
     CDS             complement(26228..26860)
                     /locus_tag="VQ846_000378"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET3075025.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(26894..27595)
                     /gene="pyrF"
                     /locus_tag="VQ846_000379"
     CDS             complement(26894..27595)
                     /gene="pyrF"
                     /locus_tag="VQ846_000379"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET3075026.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(27828..29027)
                     /gene="divIB"
                     /locus_tag="VQ846_000380"
     CDS             complement(27828..29027)
                     /gene="divIB"
                     /locus_tag="VQ846_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET3075027.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(29037..30095)
                     /locus_tag="VQ846_000381"
     CDS             complement(29037..30095)
                     /locus_tag="VQ846_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET3075028.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(30097..31449)
                     /locus_tag="VQ846_000382"
     CDS             complement(30097..31449)
                     /locus_tag="VQ846_000382"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET3075029.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(31553..32194)
                     /locus_tag="VQ846_000383"
     CDS             complement(31553..32194)
                     /locus_tag="VQ846_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075030.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTRGCGKTTFL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTTSLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(32196..34216)
                     /locus_tag="VQ846_000384"
                     /pseudo
     CDS             complement(32196..34216)
                     /locus_tag="VQ846_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(34287..34586)
                     /locus_tag="VQ846_000385"
     CDS             complement(34287..34586)
                     /locus_tag="VQ846_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET3075031.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            complement(34952..35206)
                     /locus_tag="VQ846_000386"
     CDS             complement(34952..35206)
                     /locus_tag="VQ846_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET3075032.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(35226..37067)
                     /gene="typA"
                     /locus_tag="VQ846_000387"
     CDS             complement(35226..37067)
                     /gene="typA"
                     /locus_tag="VQ846_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET3075033.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            37229..37954
                     /locus_tag="VQ846_000388"
     CDS             37229..37954
                     /locus_tag="VQ846_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET3075034.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQTFQKGIVFLDGTIC
                     KPARLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTNLKRIQFGDFTLNPDLA
                     EGNYHPLNQKELQIIKNYLEMSR"
     gene            complement(37994..38221)
                     /locus_tag="VQ846_000389"
     CDS             complement(37994..38221)
                     /locus_tag="VQ846_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075035.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(38310..38690)
                     /locus_tag="VQ846_000390"
     CDS             complement(38310..38690)
                     /locus_tag="VQ846_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET3075036.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            complement(38690..38959)
                     /locus_tag="VQ846_000391"
     CDS             complement(38690..38959)
                     /locus_tag="VQ846_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET3075037.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            39023..39931
                     /locus_tag="VQ846_000392"
     CDS             39023..39931
                     /locus_tag="VQ846_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET3075038.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            complement(39985..40740)
                     /locus_tag="VQ846_000393"
     CDS             complement(39985..40740)
                     /locus_tag="VQ846_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET3075039.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(40761..41690)
                     /locus_tag="VQ846_000394"
     CDS             complement(40761..41690)
                     /locus_tag="VQ846_000394"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET3075040.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            complement(41705..42328)
                     /locus_tag="VQ846_000395"
     CDS             complement(41705..42328)
                     /locus_tag="VQ846_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET3075041.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            complement(42321..43559)
                     /gene="hflX"
                     /locus_tag="VQ846_000396"
     CDS             complement(42321..43559)
                     /gene="hflX"
                     /locus_tag="VQ846_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET3075042.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            complement(43552..44436)
                     /gene="miaA"
                     /locus_tag="VQ846_000397"
     CDS             complement(43552..44436)
                     /gene="miaA"
                     /locus_tag="VQ846_000397"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET3075043.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKCFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            44557..44727
                     /locus_tag="VQ846_000398"
     CDS             44557..44727
                     /locus_tag="VQ846_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET3075044.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            complement(44775..45614)
                     /locus_tag="VQ846_000399"
     CDS             complement(44775..45614)
                     /locus_tag="VQ846_000399"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET3075045.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(45711..46670)
                     /locus_tag="VQ846_000400"
     CDS             complement(45711..46670)
                     /locus_tag="VQ846_000400"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET3075046.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            complement(46797..47794)
                     /locus_tag="VQ846_000401"
                     /pseudo
     CDS             complement(46797..47794)
                     /locus_tag="VQ846_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            complement(47844..48461)
                     /locus_tag="VQ846_000402"
     CDS             complement(47844..48461)
                     /locus_tag="VQ846_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET3075047.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALALELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKALMVGVSEELKTLDLTDIAGTCPYPTLLICGIKDKPNL
                     SSMRSLHKLISESQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            complement(48462..50183)
                     /gene="pabB"
                     /locus_tag="VQ846_000403"
     CDS             complement(48462..50183)
                     /gene="pabB"
                     /locus_tag="VQ846_000403"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET3075048.1"
                     /translation="MHRKTVIDFRILGERYTFTQPIKEIKTRNVAEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGKLLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            complement(50244..55886)
                     /locus_tag="VQ846_000404"
     CDS             complement(50244..55886)
                     /locus_tag="VQ846_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET3075049.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQFPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQQENPKEDRGAEETPKQEDEQPAEAPE
                     IKVEEPVESKEETKTAKGTQEKGHVGEALAQEQLPEYKVTEGTLVETSTTDLDYKTET
                     TEDPTKYTDEETVLRTGEKGSQVTKTTYKTVEGVKTDQVLSTSTEVTKEPVNQQVIRG
                     TKPIEGTLIEESLEKIPFKEVVKEDDQLKKGLEVVAQEGKEGQKKITKTFNTIKGVKT
                     EDAPKVTEEILEEPQDKILKRGTKSFEKPVLTITEIESKDLKRTSDVKYSLENPSKAA
                     IKSITLTLKKGDEIVKTLNVSPEDLTTTLTDLQYYKDYKLETKMVYDRGEGDEEEVLK
                     EEPLRIDLKKVEIKDIIRTDLIKYENQVETDETRLTSVPTDLSNYYLKVTSNDHKTNL
                     LAVQKIEESTIDGKQVYKVIAVADNLVQRNVDNRFEETYTYYIEKPKASQAGIFYDFE
                     ELIQAIQTNPAGEFKLGQSMSARNVVPKGKSYITSEFTGKLLSDGDKRYAIHNLEHPL
                     FDKIKAGTVKNINFENIDIDLSNQDNIAAIANEISGTTRIENIKAIGTITARNNVAGF
                     VNNIKEDATLENVAFIGSINSAGNNSTVGGIAGSNYMGFVNRAYVDATITANNANASM
                     LVPFVQYKAHSWLSGTKGKLTNSVAKGILDVKNTKYVGGITSKTWPYGAVQDNVTYAK
                     VIKGQEIFGSNDVNDGDANPFVSNLFGVIGYSSSEDGTGKDNKNKNKLKHLSKSEADA
                     KVASFNITAAKFVSEPYELNKLNNVSSREAAYESIQDYNADYKLAYKNIEKLQPFYNK
                     DFIVNQANKLDKNNKLNTKEILSVTPMNNNNFVTDLGDANKIIVHYADGTKDYFNLSS
                     SDEGLSNVREYTITDLGIKYTPNIVQKDHSALINGIVNILSPIELQSNPIYQKLGRTG
                     SNRVNAIKDLYLEESFDHVKANLNILVTKLVENEDHQLNNSSAAQQMILDKVEKNKAA
                     LLLGLTYLNRYYGVKFGDVNIKELMLFKPDFYGKNVSVLDRLIEIGSREDNIKGHRTY
                     DAFSEIISKTTLSENLVNFLNYNRRLFTADVNIYDWFKKATEGNVYIAERASEVDEIK
                     AAKYKVWDNLQSATHRKMILPLLNLNKSHVYLISNYSAMAVGTAEKLGHSSFDGIKNQ
                     IEKAADSYRNYFDFWYRLSLDKVKNMLVKNTVTPLWEGYQAPGGWPDKYGKRNNKEDY
                     APLRELFGPIGKYYGNNGTGAYAVIWDNPLDTRTEVNYIALSMIDEFGISVYTHETTH
                     VNDRAIYLGGYGRRSGTHAEAYAQGMLQTPVPSTWFDEYGALGINMTFYRPNDGNQWY
                     ITDPKTLKTREDIDNYMKGYIDTLSLLDYVEGDAVISKHDDKLNQLWFKKVDPIERTV
                     GSKDQYDKVRNMSSDDWKGITLNSVNDLVDNNLMTNRMMNNNIYKPEVFDPGIAYVGI
                     KMMNSIYAGNTSEGAPGAVTFKNNAFRLWGYYGYDKGFLGYASDKLKSQANKEGKQFG
                     DQYIIQKISDGRFQSFADFKKEYYKELLEKANAGFTPFTIDGETIHNYDELKSLFAKA
                     LEEDMVTNKVNGKLVQVKEKVYKKLLQQTNSFKTSIFK"
     gene            complement(55908..56471)
                     /locus_tag="VQ846_000405"
     CDS             complement(55908..56471)
                     /locus_tag="VQ846_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET3075050.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            complement(56587..58278)
                     /locus_tag="VQ846_000406"
     CDS             complement(56587..58278)
                     /locus_tag="VQ846_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET3075051.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            complement(58275..59012)
                     /locus_tag="VQ846_000407"
     CDS             complement(58275..59012)
                     /locus_tag="VQ846_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3075052.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
BASE COUNT        17516 a        12989 c        10677 g        17975 t
ORIGIN      
        1 atttattcct ccatacacac cagagatgaa cccattgaac aagtgttgaa agagattcgt
       61 aaacgtggat ttaagaataa agcctttcga actttggaag atgtcatgaa tcaacttcaa
      121 gatgtcatac aaggactgga gaaggaggtg ataaagtcca tcgttaatcg gagatggact
      181 agaatgcttt ttgaaagcag atgagtatta tatgcaattt ctttatataa aaagaccgga
      241 ttgctccgat ctttcaatag ttcatattct caatttctat tttaaaaata gctaaggtta
      301 acgtcaaatg actacgcgac ctatttcata cgataaaaat caagcactag accagcaggt
      361 ccttgaacta ataaggactc tgttccccaa tcggttacag ttggtccgtg taaaaccttt
      421 ataccaagct cgttcaaccg tttgtagttc tggtctacat cctcaacctc gatatgaata
      481 atgattcctg actgaaagtt ttccaaagga accaaatgat tttgtgacaa cataaggcag
      541 tgactaccaa tcgtaaactg agcaaaacca tcatcagcat aatctgcctt tttatccaag
      601 atatgctcca agtcagcaca gacttgggga acatttgaaa cgataatatc taattgattt
      661 aaattcattt actctcctcc ataaaaagac cggattgctc cgatctttta aagttctgct
      721 ctatgaaaat caaagaataa agtctacaag tttcatattt gattttcggc gagaggaatt
      781 atttaattgc gcgtgattgc aatccttctt cttccaagaa gagacggaat ggtacgagtt
      841 cttctgcttc gtatttttcc ttgaaggctt tgatagcttc ttctgagtga agttttggat
      901 ccaattcaag tacttctact ggaagtggac ggtgttgagt gatgcgagca tcgatgacaa
      961 cagttttacc ttctttgttc aatttaacag cttctgcaac aactgcatcg atgtcttcga
     1021 tacggtcaac tgtgaatcca acagctcctt gagcttccgc aattttagcg tagtcagcgt
     1081 ttgtgaagtc tacaccaaac aagtgtttgt ttgtatcttc gtatttgttc ttgatgaagc
     1141 cgtactcagc atttgagaag acaaggttga taactggaag gtcgtattga acgtttgtga
     1201 taacgtctgg gtagcacatg ttgaatgctc cgtcacccat gatgttccat acttggcgat
     1261 ctggattgtc tttcttagca gcgataccac caggaagggc aatacccatt gtcgcaaaga
     1321 gtggagatgt acgccacatg ttcttaggtg tcatgtgaag gtgacgagta gatgtttgag
     1381 tagtgttacc tacgtcgatt gagtagatag cgtcttgatc agcatgtttg ttgattgcat
     1441 tgtaaacttg atacaattgc aattcaccct cagttttacc ttcgagtttg ttcatgtaat
     1501 cacgccagtt ttgattgttc ttaacgtttg cacgccacca tggagttgat tcaactgggt
     1561 ttactttgtc aaggatagct ttagctgctt gaccagcatc accaaggatt gaagcgtcaa
     1621 gggcatgacg tttaccaagt ttgtaagggt cgatatcgac ttggatgaat ttttcagtgt
     1681 tcttgaatgc ttcgtaaact tcagcgaatg ggaagtttga accaaggaaa agaactgtgt
     1741 ctgcttcaaa gaccacttcg ttggctggtt tccaaccaac acggtaagca gaacctgtca
     1801 aaccttcata gttccattcg aaagcttcaa agtttttacc agttgtgatg attggtgctt
     1861 tgattttacg tgacaattca gtaatcactt caccagcttt aacaccacca aatccagcat
     1921 agataactgg gcgttcagca ttgttcaaga tttcaacagc tttgtcgatt tcaacttcgt
     1981 tcaaagcagg agcgatgaat gagcgttcgt atgaacctga accgtagtat gagttttcat
     2041 cgatttcttg gaaaccgaag tttactggaa tttcaacaac agctggacct tttttagaaa
     2101 ctgcagcacg gcaggcttcg tcaattactt ttggcaattg ctcagcgtaa gctacacgtt
     2161 tgttgtaaac agcgataccg ttgtacattg ggttttggtt aagctcttgg aaagcatcca
     2221 tgttcaattc gttaactgga cgtgatccaa ggatcgctag gaatggagtg ttatccatag
     2281 ctgcatcgta aacaccgtta atcaagtgag tcgcacctgg accacctgaa ccaactgcaa
     2341 ccccgattga gccgccgaat ttagcttgca taaccgctgc aagagcacct gtctcttcgt
     2401 ggcgaacttg taagaaacgg atatctttgt cttcagccaa agcatccatc aatgagctga
     2461 gtgttcctga tgggataccg tagattgtat ctacgcccca tgttttcaat acgttaagca
     2521 ttgctgcaga tgcagtaatt ttcccttgag tcataatgat aactctcctt caattttttt
     2581 aaacttggag aatacgatta catagaattg gaaacgttct ccaaattttt actattccac
     2641 tgtatcatat ttatgctgac ttttctaaaa atctgctcaa aactctctat tctctattct
     2701 aatacagttt tgaaagtgtt gtcatttctg ttttataaca aagaaatcta gtcattactt
     2761 ttagtctatt ttactaaaat ttaacagaag ggaactggtc agaacagata cagaactaaa
     2821 ggccatggct agacctgcca attctgggtt gagagccagt ccaacacctg aaaagactcc
     2881 tgctgcaatc ggaattccga caacattgta gataaaagcc cagaaaagat tgagtagaat
     2941 tcgatgaaag gttttcttac tcatatcaaa ggcacgaacc actcctaaaa gattattggt
     3001 tgtcaacacc aaatctgctg actcgatggc gatatctgtt ccagctccca tagcaatccc
     3061 cacatctgct acactaaggg caggagcgtc attgataccg tccccaacaa aggctacttt
     3121 ccctgactgt tgcagtttat ggatttcatg ggctttttct tctggcaaga cgcctgcaat
     3181 gacctcttca attccgattt gatctgcaat agcacgcgcc acaccagcat tgtctcctgt
     3241 cagcatgact gttcggagac cacgtttttt tagctgactg atgactagct tagcattttc
     3301 cttaggaata tcttgcaaag caagcaagcc tttgatttca ttgtcaacag ctaagaacac
     3361 aactgtctta gcttcttttt ctagttcttc tagtttatct tgataagtat tagaaatatc
     3421 catgccatcc agcattttag catttccaag taaaacttgt tttccattga ttcgccctga
     3481 aacacctttc ccgtgcaagg cctgaaaatt ttcaacagtt tgaaactcaa gtccagcttc
     3541 actcgctcgc ttaacgatag cctcagccag tgggtgttga gaagcatctt ccaaggaggc
     3601 tgccaatcca aacacttcta cttcgtcgcc gatgatatct gttaccacag gtttcccttc
     3661 cgtcaaagtc ccggtcttat caaagacaag agtttgaact ttctggattt cctgtaagac
     3721 agttccattt ttgaggagaa cccccatctt ggcactacgt cctgtcccca ccataagggc
     3781 tgtcggtgtt gcaagtccca aggcacaagg acaggcgata atcaaaaccg ccactccgta
     3841 gagaagagag gacacaaagc tagctccaag cacgaccaca ctatccctga gcaagacgaa
     3901 ccaaacccaa aaggtcatga ttcctaaaat gacaactact gggacaaaaa tccctgaaat
     3961 cttatccgtc aagtcctgaa tcggcgcacg acttgtctga gctttcttca caaaatccac
     4021 aatctgagcc aaaacagtct ctgagccaac tttttctgct ctaaagacaa gcgttccact
     4081 atgattgatg gttgagccaa tgacagtatc tccaactgtc ttgtccacag gcagactctc
     4141 acctgtcacc atggattcgt caatactaga gacaccttct actacgacac catcaacagc
     4201 aatcttttca ccgggacgca ctcgaatcag gtcgcctacc ttgacttgtt ccaaaggaac
     4261 ttggacataa ctatcatcac tcaagacttc tgcggtttta gcttgcaagt ccagtaattt
     4321 ctccacagct tgggacgtat tttttctcat tttttcctca aaaactgctc ccaaaagaac
     4381 gaaaaagagg ataaatccag cactttcgaa gtaaacaggg agaccagcaa agagagcaac
     4441 taggctatag aaataagcca ctagagttcc cagcgcaacc aaggtatcca tgttggcatt
     4501 gtgcttttta aaactggccc aagcactctg gatatatggc ttacctgcaa ctaacataat
     4561 aggcgttgtt gctagaaagg ttccccaatg catgacttga tgactaatgc tacctgtcaa
     4621 catcccaatc atgagaatca caagaggcac agtaaagata ctagtaatcc aaaaacgttg
     4681 caggagagat agagattttc gagtcttctc aacgactata tagcttccct tttgcatctt
     4741 catgccacaa gaaaattcat gtcgccctaa ttcttgaggt gtaaaacgaa tgactttctc
     4801 ctcatctacg ccgattggtt ccaagatacc ttcttcttca aacagaattt ccttataaca
     4861 gtttgaagga gtagcacgat gaaaggtaat ctcagctgga attccctttt gaagctggat
     4921 atgggctgga tgatagcctt tttcagctcg gatacggatt ttttgaatgc cattttctaa
     4981 gcttgctttc acaatttctg tcatagtctc cacctactct acaatcatct tgccgtgcat
     5041 catgttcata ccacaagcaa agccaaactc tccaacctgc tcaggcgtga tttccactac
     5101 atactcttcc cccattggca ggttcgcatg tacaccaaaa tctggaaaaa caatttgatc
     5161 cagacatggt gaaggatcct tgcggtcaaa gacaatgcgt gctggcactg atttcttgag
     5221 gacaatcaac tcaggagtat agcctcccat gacttccact cgaatctctt ggtatccgtt
     5281 tttttgctgg gctttttgtc cagatttttc aggctttttg aaaaaccaaa acaagataaa
     5341 cgcgataagg gcaatacaaa taatggttac aatactattt aacatggcgt ctcctttaca
     5401 tacaattaca tcttacttct gttacagcac ttgatttctt ctctgaaatc acagcttcca
     5461 agtcttccaa gtcagtctga gtaaattcac attctacaat caagtcagcc aacaaattcc
     5521 taatcctacg ggaacaaacc ttgtctttga tatcttggac aagtaaatcc cgactttggt
     5581 ctagagttaa aagcgctgaa taaacaaagg acttgccttc ttttttccga gtcagacact
     5641 ctttctcaac caaacgagcc aaaagagttt gaatggtcga cttggaccag tcgaaccgct
     5701 ctgccaaaac cctaatcaaa tctgtactgg tctgctcccc ctgcatccaa ataatcttca
     5761 tgacctgcca ttctgcatct gaaatctgca ttaccatacc tccaaaatct acatttgtca
     5821 attacactca tcagtatact cttaaaatct acatttgtca attatagaaa taatattttc
     5881 ttcgaaaaat agaattttaa tcatttgaaa aacgatttgc agtcaaatat tactatataa
     5941 acaataaaaa tatgctatac taaagaaaaa agaaaacaac cactaggggt gcgtaaagct
     6001 gagattaacg actgttagat ccctctgact caatctaggt aatgctagct gatggaagtg
     6061 gaaatgataa tggggactag cagtcttcta ttgcctttct aaaacagact agcttgttct
     6121 taagaataca aacttcagtt ggttgggagg ttttagatga cttatttacc cgttgctttg
     6181 accattgcag ggactgaccc tagtggtggt gctggcatta tggcagattt aaagtcattc
     6241 caagcgagag atgtctatgg aatggctgtt gtaaccagtc ttgtcgctca aaataccaga
     6301 ggtgttcagc taatcgagca cgtttctcct caaatgttga aagcccaatt ggagagtgtc
     6361 ttttctgata ttccacctca ggctgtaaaa actggaatgt tggctactac tgaaatcatg
     6421 gaaatcatcc aaccctatct taaaaaactg gattgtccct atgtccttga tcctgttatg
     6481 gttgctacaa gtggagatgc cttgattgac tcaagtgcta gagactatct caaaacaaac
     6541 ttactacctc tagcaactat tattacgcca aatcttcctg aagcagaaga gattgttggt
     6601 ttttcaatcc atgaccccga aggcatgcag cgtgctggtc gcctgatttt aaaagaattt
     6661 ggtcctcagt ctgtggttat caaaggcgga catctcaaag gtggtgctaa agatttcctc
     6721 tttaccaaga atgaacaatt tgtctgggaa agcccacgaa ttcaaacctg tcacacccat
     6781 ggtactggat gtacctttgc tgcagtgatt actgctgaac tagccaaggg caagagtctt
     6841 taccaggcag ttgataaggc caaggccttt atcacaaaag ctattcaaga tgcccctcaa
     6901 ctcggtcatg gttctggtcc agtcaaccat acaactttta aagattaaga aaaaaactct
     6961 ctagttccca ctttaaggga attagagagt ttttatactc ttcgaaaatc tcttcaaact
     7021 acgtcagctt ccatctgcaa cctcaaaaca ctgttttgag ctgacttcgt cagtcttatc
     7081 taaaacctca aggcagtact ttgagcaacc tgcgactagc tttctagttt actctttgat
     7141 tttcattgag tattaattag gaaagaatgt catgcaactt tgttaaaaag gcttgcgttt
     7201 ttgcctcaat atcttctgct tgcatcaaat cacgtacaac agctacacca gctatgccag
     7261 tgcccataag ctgatcaata ttctccgaag tcaagcctcc aatagcaact actggaatgg
     7321 caaccgtttg gcaaattgtt ttcaaggtcg atatcagagt aatgggcgca ttttccttgg
     7381 tggtggttgg gaaaatggct cctgtaccca agtaatctgc acctgatttc tccgcttcca
     7441 gagctctttt aaccgtttta gcggtgacac cgaggatttt ttcaggaccc aagactttgc
     7501 gagctaccga aactggtaat tcatcatctc cgatatgcag acctgctgca tcaaccgcaa
     7561 gacaaacatc caaccgatca tcgattatca aggggacctg ataggcatct gttatttcct
     7621 tgacttgttt tgccagttga taatattgat tggttgtgag atttttttct cgcaattgga
     7681 ctatggtaac ccctgaacgg caggccgtct caacttttgc aagaaagctt tccacggaat
     7741 cttgatagcg attggttacc agatatagtc taagtgcttc tctattcata aacctctcct
     7801 ttgatggtat ctagccaatt ttcatctctt cttaggagcg aaagctgatt gagtacttgg
     7861 taacgaaatt cttccaatcc cattccttga acaactattt tctcagcagc gatattgaga
     7921 taagagactg ctaagcaaga agcttcaaaa ccagtctttc cttggctgag aaaaacggct
     7981 gttaaggctc caaccaagtc tcctgtccct gttatccagt ctaattcagt acagccattc
     8041 tccagtacag caacttgatt ctccgaaaca ataaggtcct tggggccagt aactaagaaa
     8101 gacataccag gataggtctg acaccagtct ttcaagactt gaagcaaatc ctccgtttct
     8161 tgatctttag cactcgcatc gaccccaacg ccgtggtgct ttaatccaac aagacttcga
     8221 atttctgaca tgtttccttt aaggaccgta ggtctatagt ctaaaaggtc tttaactaag
     8281 ctcttacgaa tggatgaagt cgttacgcca accgcatcta ctaccatcgg gagagaagat
     8341 tggtttgcat acgaagctgc catgcggatt gctttttcct tctcagctga caaatgcccc
     8401 aaattgatga agagagcctg actttgctta gtaaaatcaa gaacttcacg gggatcatct
     8461 gccatgacag gtttgcatcc cagagccaaa atcccatttg ccagcatctc acaagaaatc
     8521 tcattggtca tacagtgaat gagggaacta gagcctatag gaaaaggatt tgtcaatgcc
     8581 tgcatcattc tatcctttca gcaaagaaat atccttgcac ttttttaaag aattcctgct
     8641 tgattaaaaa tctaaatgca ataaaggaaa tcgctgtacc aatcaaggtt gctccgaaaa
     8701 atcgaggagt gtagataaac caactaagct tagcagccga tcctgtaaag agcaccataa
     8761 caggatagga aacaatagaa ccaataatac ctgttcccac aatttctccc aaggcagaaa
     8821 agtaaaattt tcgaccgtac ttataaaaga gacctgctag aagggctcca aaagtcgctc
     8881 ctgtgagagc taaaggcgga atcccttgag tcgtcatacg gataaaggct gtcactgtag
     8941 ccatagccaa ggcataaaca ggtcccatca tgattcccgc tagaatattg actacactgg
     9001 acatcggtgc cattccctca atccgaaaga taggtgtaag gactacatca agggcaatca
     9061 tcatagataa aatggtcaat ttgtgaactt gtagttggtg ctttctcatg tttctattct
     9121 tctccttttt ctaaagactg taaatcgctc ttccatgtct ggtgttggta agccatctcc
     9181 caaaacttag cttccatatg aacactgatg tggaaggcat ctagcatttt ttgcttatct
     9241 gtctcatcac tttctcgata aagctgattg accagtgctc cctcctctct gatctgttgc
     9301 tctaactcat ccgtaatata agtttcaatc cattgttgat agagaggatt tggtgatggt
     9361 ttaagattaa gtgatttgcc tatatcatgg tataaccaag gacaaggaag caagcttgca
     9421 aaagctatgg ctaagttcgg ttctgcaaat tgcctataaa tatgagaaat gtaatgataa
     9481 caggttggag cgattggatg ttgctccatt tcctggtcgt tgatttccaa ttccttgaaa
     9541 aattgttggc gaataaataa ctcaccctcc actaaaccct gagcattttg tttcaagagt
     9601 cttttcatct cttggtttga agtcttatca gccaaaagat gatagatttc tgagaaagcc
     9661 ttcagatagt aggcatcctg aatcaggtaa tagcggaaaa tggcaggttc taaattcccc
     9721 tcttgtaatt gtaaaataaa gggatgatga aaggaagcct gccaagcttt cttggataat
     9781 tccatcgcaa tatctgtaaa ttccataata actcctttat aaaaatagac tggtttgaag
     9841 caataaaaag aaaagcaggt agattaattt tgttttttta ggaatataaa aagtccgata
     9901 gctattcttc aactgtgcat gttcgtcata tccgtgagca gatagagctc tcaggtaaag
     9961 atggcgccac ctaaagactg tcatcagaac cttactgtaa atcaagggcg accaaaaatg
    10021 tagtttttga ccacgtaata ggcaagcttc tttgagggac ttgatttctt gctgaatgag
    10081 aggaaaagaa ttgaatacca caatcaaggc ataggaccaa gagcgtgata gccccttttg
    10141 agccaagtac aagagaagct cttttagtga aatagaggaa acaaagacaa ggccgataca
    10201 aactgtcaca aaggccctcg ttccaagcat gactgcctgt gaagcatctc cgtgtaactg
    10261 aactgcccag tagttggcaa aagatggtaa aatggcaagt atgatcatcc aagctaacat
    10321 tttaaatcga cggtaataga gcataaagag aatacaaaat gcgactaccg aaagattcag
    10381 agcaatcgaa ggaatgaaag atgtttccaa ggataaaatc agcaagaaga gactgataat
    10441 cggtgtctgg gttgctactt tgaccatact atctcacctc cccttgggta ttgctactct
    10501 gagatgtaag tggtttggta atggtcactt ctttcacatg ccgaagaccc tgactagtca
    10561 tctcaatcca ataatcaacc acagaaatca aagggtctaa acgatgacta atgagcagaa
    10621 aacttcttcc ttgattcctc tcctccacaa tccacttgca aaaataatgg caggctctat
    10681 catccaaacc tgcaaaaggt tcatctagca agatcacgga agccttactg gtcaagatgg
    10741 tcaggagctg aagaattttt tgctgaccac cacttaattg atagggactc ttatcgactg
    10801 cctgctccaa atcaaaatat cgtaaagctt gaaaaatccg ctgatttctt tcagaatcag
    10861 gtccatctaa ttgaagctcc tctcgcagac tgactcggat aaactgcttc tcagcttcct
    10921 gaacaacacc cgtcagatca cggtacaaac tctttttctt tttcaggact gtccccttcc
    10981 atgcaatgcg ccccttatac ttttgaaatt gaagaataga ccgaaagagg gttgatttcc
    11041 cgacaccatt gtcacccagg atacaggaaa tcccttgata gaatgtgaaa tcagcaattg
    11101 aaaagagggg gcgattacca agctcaccag tcacacggtt catatggaat aattccgggc
    11161 tagaagcaac ttcctttgaa gcaacctgtg tcatctcata ggaagggatt tgatttattt
    11221 cccttagttt tccgtctctt agctcaacca tatggtcgat ataggcttta tagtcagata
    11281 aatcatggtc gcacaaaata actgtcttcc catcaagagc caactctttt agaatctcca
    11341 atatctcgat tctgctcttg cggtcaatgg aagcgaaggg ctcatccaag agatataccc
    11401 taggattcat ggcaaagagg acagccagcg ctgctttttg cttttcccca cctgataggt
    11461 gatgaatggg acggtgtaag attgcctcgc aacgacattg ctggacaacc tctgctattt
    11521 tagaatcaat ttcctgaagg tgatagccga tattctccaa ggtgaaaatc aactcctcaa
    11581 acaagttctc catggtaaat tgatgattgg gattttgcaa gagaatgcca accttctgga
    11641 cacgtgcgat gatagaaagc tgactgacct cgctcccatc tatcaggact tgaccactat
    11701 agggaagaga actgacttgg gcaatcattt gaaagaggct ggattttcct gaaccactgc
    11761 tcccaactaa caaggtaaag gcttgcgcat gaaaagtaaa atcaaacggc tcagagaaga
    11821 ttggggactg aatcgctcgt agttccagac ccatctatgc ttttcctcca gttgcaaact
    11881 gatgatagag tttgacaatg gcacgaacca agatggtaca gaagaaataa acagaaataa
    11941 aacgtaccac aagcaaggaa aggacaaacg gaagggaaaa ggcgtagtaa cctaacttaa
    12001 tgtattcata gacaaagcta acaagcgtaa tcccaatact attagcagtt agagagagcc
    12061 aactttcata gcgattctta gttacgataa aaccaaattc acttcccaaa ccttgaacca
    12121 agccagacaa aagagctcct agaccaaatt ggctaccata aaggacttca gcaagcgcag
    12181 ctagcacttc tccaatcgtt gcacttccga ctctcggaac aaagatggca gcaatgggcg
    12241 cagccataca ccagagaccg aagaggattt cattggcaaa ggcctgcaaa ccaagaggtg
    12301 ttaagagtag actgagaata ttatacacat atcctgaacc aacgaaaacc ccaccaaaaa
    12361 agatagacaa gaaagcaagc aagataacat cttttaactg ccattttttc aacataaaaa
    12421 actccttttt ttaaagaaaa gtggggcact caagaagacc gacctaaata ctttgtatag
    12481 cagactgaat ttagaacagt acacaagaac actaaaatat ttctagaaat taatttgaat
    12541 tttctaattg atttgttcgc atcttatttc aatctactat atcatcttca tccagtttcg
    12601 taaaagaaaa aactctaatt acagatacga attagagttc agcttacaag attagacagt
    12661 tcttttcgac atacgaaaaa aacatttcac atttcccttc gccagtctta actgtatcag
    12721 gttcaatggg tatcatctca gcctaaagca ccccaaatgc ctttattatt taattatgtg
    12781 attattataa cacacatttt atactagttc aagaaattga actggaaata cagccttgca
    12841 ctcacaaaga cagcagatct ttcttttgca aaaaacaaat gacctgtttg atgaattagc
    12901 tattcaagct gaatctagac atagcttttt aaaaaaggaa aatcctactt acttagaatc
    12961 caaggataga tatctattgt tcactcattt cccgaacagt tttttctata ttttttgcat
    13021 acgatattgc cgaaatgatt gaaacgccgt caacattggt ctttataatg tctttaatat
    13081 gtttcgtctg tatcccacca attgcaacta aaggcatttg tggcaatagt tttctcatca
    13141 attcaagacc ttcataacct atagcaccac cagcatcatc ctttgactgg gtatcaaata
    13201 caggaccaac accaacataa tctacatatt caacttttga ttgttgaaat tcttcctcgt
    13261 tttttataga aagaccaatt attttatctg gcatcaattt tctaatttca tcaacaccaa
    13321 tatcatcttg acctacatgt acgccatcag cgtcaatttc cattgctaaa tctatatcgt
    13381 cattaacgat aaatggaaca ttgtattttt tacaaagttc tttaatttgg atagctagct
    13441 caagtttttc taagccttct aaagcaccct cacctttttc tcgaaattga aataaggtta
    13501 taccaccttt taaggcttcc tcaacgactg tatatagatt ttttccttgg caagtagtcg
    13561 ttccacaaat aaaatatagt tttagtaatt ctttatgaaa catcttactt cactcttttg
    13621 aatttcttga catcttcatc tgtaatttca tataaggcat ttataaattc aactttaaat
    13681 gttccaggaa gatgtccatt tggacgtttt tccgccattt ctccagcgat attgtaaacc
    13741 aacattgctg tttctaatga tttcaattct tgaccttttt caagtccgat aaagcttgct
    13801 actacagctc ctaataagca tcctgtccca atgactttcg gcatcatagc actaccatta
    13861 tgaatcgtta ccacttctcc attaacagca atggcatcca cttcacctgt tactactatt
    13921 ggaatattga acttctcatt tgctgctaga gcaatttcgt caatattatc tacgcccgca
    13981 ctatctactc ctttagatgc cacatctatt cctactaaag aggcaatctc tccagcattc
    14041 cccctaatcg cagctagttt gtaattgttg attaaatcat ctgctacttt ttttctatat
    14101 tctcctgctc cacaggctac aggatctaaa actgctggga cattatattt ctctgcaatt
    14161 ttcagagcag cttggtataa tttccaattt tcatctgtca atgttcctat gtttattaat
    14221 aaaccaccag catactttaa taagtcctct aaatctgctg gaaactcact catggctggt
    14281 gaggcgccca gtgctactaa tccatttgct gtgaaatttt ttactacatc attggtgatg
    14341 caaatgacca aaggtgcttt ttcttttaat aattttaaac ttgtcatatt gaaatccttc
    14401 cttttcactt tatacgatct actaatttcg atttatcttt agttgagaat ttttttcatt
    14461 tacattgaat gatttatact caatgaaaat caaagagcaa actaggaggc taaccgcagg
    14521 ttgctcaaaa cactgctttg agcctgtaga taagactgac gaagtcagct caaaacactg
    14581 ttttgaggtt gtggatagaa ctgacgtggt ttgaagagat tttcgaagag tcttacctca
    14641 tcaaatttgt aaatatcatg agccttctct agacatcgta accaatatca aaaaaagcta
    14701 attctaaagc gactgcttga ttccagcgtt gctgaagttc tgtcaaatct tctcgatttt
    14761 taccgacacg attgagttca tcaaccagaa attgaactca ctctgcaaag aaaggacctc
    14821 tgtggagatt gatccattcc gaatgaatat agacttcagg tagagccaaa tctttagaac
    14881 cccagtctaa atagagacct tctgcaatga ccagcataac caaaagatgg gcatagtctg
    14941 atgaagccac tgcccaatac attagatcct gaaaggcttt tgttacaggg tgcaaagtca
    15001 cttctagata gttattctct gctactttta actctttaaa agccttttgg aaataaccat
    15061 cttcatctgc ttcaagaaag cctagttgct tggcaaaacg aagcttggat tcaagtttat
    15121 ctgcgtgagc tacgcaggca cccagcatgg ataagaaggc atcaaagaag tgataatctt
    15181 gaatcagata gtcctttaag accttattct caattgtccc cgcaaaaagt tccttaacaa
    15241 aacgatgatt gattgcagcc tgccaatcct tctgactgct ttttaataat tctccaacag
    15301 tcaaacctgg ctgaaatgca tagtcttgtg tttccatatt tacttctcct ctctttactt
    15361 gttagtaatt aataaaacag caagaaatat caagcaaaat cgtaattcca cttgatcctt
    15421 ttaaagcaca tcgagagcat ttacagagag ctaactaaac aagcctatcc agtttatata
    15481 aacaaaaaac tccaattaca atcaagaatt agagttgact tacaagatta gaccgttcat
    15541 ttcaccatac gaaaaaactg ttcacatttc ccttcgccag tcttaactgt atcaggttca
    15601 atgggtatta tctcagccta aagcacccca aatgtctcta ttatttaatt actgaaccag
    15661 tatagcaaaa aatgaaagcc ctagcaagat atttgaccga aaaatatctt tatatacaat
    15721 atattgaaac tagaatagta cacctctact tctaaaacat tgttagaaat cgatttaact
    15781 gtcctgattg atttgtccta ttcttatttc attttactat agttttcgat agcgatttat
    15841 tcttccaata cacgaagaaa aacctccaca ttcagtgaag gcaatctgtt ttatcaatac
    15901 aattttaagt cacgagggtc aactgggaag gttgggttgt atggattgtg acggagctta
    15961 aagtgtttga catcttcaat ggtctgagct ccagacaatt gcataactgt cttcaattcc
    16021 gcattcaagt gttcaaagac ttgacgcaca ccgacactac caccgagagc caagccataa
    16081 ataacagggc gaccaatagc taccaagtct gctcctgaag ccaaggcttt aaagacgtgt
    16141 tgaccacgac gaacaccaga gtcaaagaca atcggcacac gtctatcaac tgcttctgcc
    16201 acttcttgaa gcgagtcaaa ggcagctggt ccaccgtcga tttgacgacc accgtggttg
    16261 gttacccaga taccagaagc tcctgcagca agtgaacgtt caacgtcctc acggcattgt
    16321 ggtcccttga catacacagg aagaccagag tattcagcga taaattctac atcgcgtgga
    16381 gacaagcgtt gtttagctga tttgtaaaca aagtccattg atttaccagc accttctggc
    16441 aggtattctt caacaatcgg catgccaact gggaagacaa aaccattacg cttatccact
    16501 tcacgattcc cccctacagt agcatctgcc gtcaagacaa tcgctttata accttcagcc
    16561 ttcacacggt ccatgatgtg gcggttgata ccgtcatcct tactaaagta aaattggaac
    16621 caatggggtg tcccttgaag ggcttcagaa atctctggaa ggtcaacagt agagtaagag
    16681 ctggttgtat aaagagaacc aaactcatgc acaccacgcg cagtcgccac ttccccctgt
    16741 tcatttgcca atttatgagc cgcaacaggc gccataatga ttggtgaaga tagtttttca
    16801 cctgcaaatt caatctctgt acttggattt tcaacatcgc aaagtgtatg aggaacgatg
    16861 agtttgtggt taaaggcgcg gatattctct cttaaagtga aagtatcttc cgccccacta
    16921 gcgatatagc caaatgctgc tttaggaata acttgttgcg ccattggctc caaatcatag
    16981 gtattgatga agtctacatg accttctgca ttgcttgttt tatatgacat aaaatgccct
    17041 ccttgattaa gtaagcgttt gctttttact acacaactat aataactctt ttttcaatca
    17101 ttttcaagag ttttgcttaa aaaccatctt attttttcat acaatatact agccctactt
    17161 tccttaaact ctaaacctta gtctatatta agtgaatcgt tgagattttt tgctgccagg
    17221 catttgaact acttttttat ttgcggattc tatctactaa tactataaaa aaaacaagtg
    17281 aataagacac cagaacatcc tctaatagga cctatggact tatatcagtc ccatatatac
    17341 tgtttaagca agatactatc ctagttgacg ctaacttcct atcccactta ttaatttttt
    17401 atttatagtg aatctgaaca aatcaattgg ggaatccaag tcaatttcta gtactttttg
    17461 aacagtttca gcgtactatc gtaaaatgaa ataaaacatg cacaaattga ttagggaatt
    17521 taaaccaatt tctaacaatg tcttagaaat caaagtgtac tatttcaact tcaatatact
    17581 acacttcatt ttctccttaa attcagtctc tttttgaatc aaaaaatcct ctggtaagag
    17641 ccagagggta agaatttatt tcattgttgg gaagagcaat acatcacgga tagttgttgt
    17701 atcagtgagg agcatgcaga gacggtcgat accgattccc aaaccacctg ttggtggcat
    17761 accgtattca agagcctcga tgtagtcata gtcgatgcct gtcgcttcat catcaccaag
    17821 ttctttagct ttagcttggg cttcaaaacg gctaagttgg tcgattgggt cgttgagctc
    17881 agtaaaggca ttaccgtact ccttagtcat gataaagagc tcgaaacggt cagtaaagcg
    17941 ttggtcttca ggatttttct tagcgagtgg agatacagct actggatgtc catagacaaa
    18001 ggttggttgg attaaagttt cttcaacaaa ctcttcaaag aaggcattga tgatgtgacc
    18061 aacctcagtg tagtgtttct caactggaac tttcttctca gcagcgatag ctttagcttc
    18121 tttcaaagtc atgtcttgcc agaaatcgac accagtaatt tctctgatag catccaccat
    18181 atgaacacgc ttaaatggtt cgttaatctt gatttcagta ccttggtagt tgactgggcc
    18241 atcgccctta actgatttag cagcatgttg gataatgcct tccgtcaagt ccatgatatc
    18301 ttggaagtct gcataagctt ggtaaacttc gatagaagtg aactcagggt tatgagtagc
    18361 atccattcct tcgttacgga agatacggcc aatttcatag acacgttcca taccacccac
    18421 gataaggcgt tttaagtgaa gctcagtcgc gatacgaagc accatgtcaa tgttttgggc
    18481 attgtggtgg gtgataaatg gacgggcagc agcaccaccg gcttcattat gaagaacagg
    18541 tgtttccact tcaaggaatc ctttttggtc aaggtaacga cggatttcag agatgatttt
    18601 tgaacgagtg acaaagcgtt caaagctttc acgattagaa atcaagtcaa ggtaacgttt
    18661 acggtaaatt gtttcaacgt ctgtcaaacc atggaatttc tcaggaagag gacgaagagc
    18721 cttagacaag tgtgtgatgt gggttgcctt gatagagagt tctcccatat ccgtacgcat
    18781 cacttcacct tcgacaccaa ggaagtcacc aaggtctgct tttttgaaga tttcgtagtt
    18841 ttcttcaccg acagcatcct tacgaacgta gatctgaatc tggccttcgc ggtcttgaag
    18901 gtgggcaaaa ccaacttttc ctttaccacg tttggttacc aagcgtcctg cgatagtagc
    18961 tgtttcgttt ttatcgtgta attgttcttt atcgaggttg gcatatttat cttttaattc
    19021 ttgtgaattt gcagtacgtt caaaacgttt tccgaaagga tcgattcctt gttcgcggag
    19081 cgcagccatt ttttcacggc gaacgatctg ctggtcattt agttcttcca tatgttctgt
    19141 agacatggga tcctcctagt tttactcaaa attgaatacg atgagtcatt ttttgcgata
    19201 ctcccatttt atcataaata agcaagaaat tcacggataa tctttaaaaa ctaaaaagaa
    19261 cctgacgcta aaatcactgg aaagaagtga aagtatcttc cgccccacta gcgatatagc
    19321 caaatgctgc tttaggaata acttgttgcg ccattggctc caaatcatag gtattgatga
    19381 aatctacatg accttctgca ttgcttgttt tgtatgacat aaaatgtcct ccttaataag
    19441 taagcgttta ctttgtatat tataaaaata tattaactct tttttcaaaa cttttcaaat
    19501 attttgtttg gaaatttcag aaattttatg tctatgataa aaatccttat aacggcaata
    19561 aaaaatagat attatccaaa gaagatttta agtgctacaa taactgtatt atttctagat
    19621 gggaggttct atttttggat tgatccattg ttgaacaata tctaccacta tatcaaaagg
    19681 cattctttct gaccttgcat attgcagttt ggggaatttt gggatccttt ctgctcggtt
    19741 taatcgttag tatcatccga cattatcgaa tccctgtttt agcgcaagta gcgacagcct
    19801 acattgaatt gtcacgtaat acgccccttt tgattcaact cttctttctc tacttcggtc
    19861 ttcccctaat cgggattgtc ctatcttcag aagtctgtgc aacgcttgga cttgtctttt
    19921 taggaggctc ctatatggca gaatctttcc gaagtgggct ggaagccatc agtcaaaccc
    19981 agcaggagat tggcctcgct attggtctga cacctctaca ggtctttcgc tatgtggttc
    20041 ttccgcaagc aacagcggtg gtactcccct cctttagtgc caatgtcatt ttccttatca
    20101 agaaaacctc tgttttctca gcagtggctt tggccgacct catgtacgtc gccaaggatt
    20161 tgattggtct ctactatgag acagacattg cgctagctat gttggtagtt gcttatctga
    20221 tcatgctgct acccatctca ctggtcttta gctggataga aaggaggacc tgccatgcag
    20281 gattcgggaa tccaagtact ctttcaagga aataatctcc tgagaatctt acaggggttg
    20341 ggcgttacga ttgggatatc catcctgtct gtcctcttat ccatgatgtt cggaacagtc
    20401 atgggaatca tcatgacctc ccattctaga atcatacgat ttttaacacg attgtatctg
    20461 gaatttatcc gtatcatgcc ccagctggtg ctactcttca tcgtttactt tggcttggct
    20521 cgaaacttta atatcaatat ctcaggtgag acttcagcta ttatcatttt taccctctgg
    20581 ggaacagctg aaatgggaga cttggtacgt ggagctatca cttctctccc taaacatcag
    20641 tttgaaagtg gataggcact cggcttgact aatgttcaac tttactacca catcatcatc
    20701 ccacaagtct taagaagact gctaccgcag gctatcaatc ttgtcactcg gatgattaaa
    20761 accacttcat tagttgtttt gattggggtt gtggaagtga ccaaagttgg acaacaaatc
    20821 atcgatagca atcgcctgac catcccaact gcttcatttt ggatttatgg aaccattcta
    20881 gtcttatatt tcgcagtttg ctaccctatt tccaaactat ccactcactt agaaaaacat
    20941 tggagaaact aaatgtctga aactatctta gaaatcaagg aactaaaaaa atccttcgga
    21001 gacaatccca tcctccaagg actttctcta gaaatcaaaa aaggggaagt tgttgtcatc
    21061 ctagggccat ttggttgtgg gaaaagtacc ctccttcgtt gcctcaacgg cttagaaagt
    21121 attcaaggtg gagatattct tctggatggt cagtctatcg ttgaaaataa aaaagatttt
    21181 cacctagttc gccaaaagat tggcatggtc tttcaaagtt atgaactctt tccccatctg
    21241 gatgtcttac aaaacctcat cctaggccct atcaaagctc aaggaaggga caagaaagaa
    21301 gtaacggaag aagctttgca attactagat cgtgtcggtt tactggataa acaacatagc
    21361 tttgctttgc ccgtcaatta tctggtggac agaagcaacg tgttgcaatt gtccgtgccc
    21421 tcctaatgca tccagaaatc atcctttttg acgaggtgac tgcttcgctg gatccagaaa
    21481 tggtgcgtga ggtgctggaa cttatcaatg atttggccca agaaggccgt accatgattt
    21541 tagtaaccca cgaaatgcag tttgcccaag ccattgctga ccggattatc ttcctcgacc
    21601 aagggaaaat cgctgaagaa ggaacagctc aagccttctt taccaatccg caaaccaaac
    21661 gagcccagga atttttaaac gtctttgact ttagccaatt cggctcatat ctataaagga
    21721 gattcttatg aaactattca aaccactctt aactgtttta gcacttgcct ttgcccttat
    21781 ctttatcact gcttgtagct caggtggaaa cgctggttca tcctctggaa aaacaactgc
    21841 caaagctcgc actatcgatg aaatcaaaaa aagcggtgaa ctgcgaatcg ccgtgtttgg
    21901 agataaaaaa ccgtttggct acgttgacaa tgatggttct taccaaggct acgatattga
    21961 actagggaac caactagctc aagaccttgg tgtcaaggtt aaatacattt cagtcgatgc
    22021 tgccaaccgt gcggaatact tgatttcaaa caaggtagat attactcttg ctaactttac
    22081 agtaactgac gaacgtaaga aacaagttga ttttgccctt ccatatatga aagtttctct
    22141 gggtgtcgta tcacctaaga ctggtctcat tacagacgtc aaacaacttg aaggtaaaac
    22201 cttaattgtc acaaaaggaa cgactgctga gacttatttt gaaaagaatc atccagaaat
    22261 caaactccaa aaatacgacc aatacagtga ctcttaccaa gctcttcttg acggacgtgg
    22321 agatgccttt tcaactgaca atacggaagt tctagcttgg gcgcttgaaa ataaaggatt
    22381 tgaagtagga attacttccc tcggtgatcc cgataccatt gcggcagcag ttcaaaaagg
    22441 caaccaagaa ttgctagact tcatcaataa agatattgaa aaattaggca aggaaaactt
    22501 cttccacaag gcctatgaaa aaacacttca cctaacctac ggtgacgctg ctaaagcaga
    22561 tgacctggtt gttgaaggtg gaaaagttga ttagtcatta actcttaaaa ggaactggat
    22621 tttaagatcc aatccctttt taagatttta cctataacat cctgagtcta tctaagatgt
    22681 tcaatctgaa cacagtgtac atactttatc ttctattgca tatactttat cacataagat
    22741 acgaatatcc tcttcactat gactagcaat caaaattgtt gtcccttttt cactagagag
    22801 ctttctaaac aatgttctca tattttctac acttgattta tccaaggcat tcataggttc
    22861 atctagtaaa agaatagagg gattctccat aattgcttga gcaatcccta gctttttcct
    22921 catacctagc gaataagttt taactttctg gtctttttgc tcatatagac caactatttt
    22981 cagtgtatca ttgatttcct gattaccaac tactcctcgt atgcttgcca aatattgtaa
    23041 attcttaaag ccactataat aatttataaa accaggttct tcaatcaaag ctcccaaatt
    23101 agctggaatt tttctctcag gaacaatatt ttccccattg attaacactt ctccataaga
    23161 cggactatat aaaccagcta ttaatttaaa caatacactt ttccctgagc cattcgcacc
    23221 agtaattcct ataatttccc cctgtttaca actaaagtta aggttttgaa aaacacatgt
    23281 tttttttaat tccaactgaa tattttttaa tgtaattatt tcattcattc tataaacctc
    23341 ctcttttgac gagtgaaata gaaaatgctt ttaaaaagaa agactaaaaa tagcaactga
    23401 agaaataaat ctcgtcctat atctccattc cctcgattta aaatataaaa tagataatta
    23461 gttcgatttc ctacaaatag accaccaaac acaatcatga gtaaaaagaa gctaacacaa
    23521 gaaaagttca tttgctcatt cgctcctagt acgtataaca taaagcaaaa caagactaac
    23581 acacaagcat tcaaagaatc aactataaac aaaactccta tatcccagtt tggtaactgt
    23641 aacataaatt gaatcactag aacttttacc atgattaaaa tgagtccata aagaaaacag
    23701 gtccaaatct gatacctaat ccagagctta atagaagaaa atcgaatcac ttccataaag
    23761 aaatcggatg ctcttctacg atatacaact gctagaaaaa atgtatgaat aataaaccaa
    23821 tgaagcaacc atatagataa gactaaaaaa gagttttgac tcagattagg cccttttaaa
    23881 atttctataa caagtctatc atttccttct aaccaatacc ataatcctaa acaaatcaat
    23941 atccaaaaaa gaacataacc ccaaaagtac ttggtataga ttatctgatt caacctccac
    24001 atgtgactac ttaactttcc catttccaac ttttacactc tcctccagtt tctatgaaca
    24061 gaaaatacag tcaatggaat tatgatagat agtaatgtaa gccaataaat caatgcatac
    24121 ggattggaat ttaccatata ctgaaaaatt ggcaataatc caattatccc attgtccaac
    24181 tgataaatcc agaataagga ttctacaaat aaaaaagtaa acagaaatac taagctcatg
    24241 tttgattttt taaaccaaga aaaactgcta atagtaagta aggataaaaa gaaatagtat
    24301 gctatataag agaaaaaaaa tcctataaaa aaactagcat tgtttgcaat acttatacca
    24361 taaaattctc ttaaaaaatc aacctccttt atctccaaag agaagctaaa accattacta
    24421 aatgcaatca gaaaaatcaa taaaaataaa gtcgccgtcc aaatccccgt actaagagct
    24481 gctaatttga aactaaaact ggtaaagtgc ttaattgatt tcagacgaat acgacactcc
    24541 aacctattaa aatagttatt catcaaataa aaaaagaata atatatatgt gaacggaaag
    24601 caatatactc cagtcgtcat atcttgaagt aaaactaaga tccattctaa tacatttgga
    24661 tggattgaat attggcgaca gcgcaataaa tatactgtac tagataaaac acaggatagc
    24721 agtaatataa aataaaccaa tactgctaag aaatcttttt tataaaattg aacaaattgt
    24781 ttcattatac atagtcctct gaatgtagaa aaaatgtacc ataaacaacc aaacaactaa
    24841 caaataaaat aaaagcaaga tgcccactaa ctaaggaaag actgatatct ttctgatatc
    24901 ccaaagctag cgttgtcaca ggttctaagt aagatagccc taaaatagcc cataaaatac
    24961 caccaaccat catataggca actgggatga aaatagctcc taattttttc ttcactagca
    25021 aagcactagc tagtccaaaa atagagaaca cagcgcccca aactccatac cagagagtcg
    25081 tcacaagact atagagaaac tgattggaat caaataattc ttttaaggca ccactataat
    25141 ctccaatata aatttcctga taaggagtca ctaaaagatt aattcctaat aataataaat
    25201 aggggagaaa aaagactaga aaagaagaaa taattgcagt actacctaca atagctagat
    25261 acctcttttt agaaattcgg cacaattgtg ctgttagaaa atgactctca gcatcctcta
    25321 ttatctgact agaatagggc agtgtacaga taagtgcagc tactaggcta atcggtgaaa
    25381 atcctcgaat agaagaagcg gcaaaaaaaa tcgataaacc ttcaatttta taaataccat
    25441 tgaaagcaag gaaatttcct aaactcatgc aaagaagggt caaaaataaa gacatataaa
    25501 accgaggtga ttgaacgact cgtacaagat tacaaatgaa aaattccatc cttactcctc
    25561 cttataataa aaatagggtg tagcattctt ttttcatgct acacccacaa tcaaccatct
    25621 ttaaggctta ctctgacaag taagttaata agaatctgga ctccaagaac ctgaagtatg
    25681 aattcttaca taatttccaa attgtggcgc catagctaat ctagtaccag aaccaatata
    25741 attgtcacca cctccattat agtacatgac aatcctagag ccagacccca atgaatatac
    25801 cggggtaata tctgacccac tataggcgct acgaatagag gtatttaacc ttttaccgcc
    25861 accagtgctg tcactattat taattccagc agaggcgttt tctttctcaa gctcactagt
    25921 tgtatccgaa ccccaacgcg gcaacgttaa atcataattt ccaatctgtc tagctaaaac
    25981 tgtagggaga aacaaactag atgcaattgt aactacgcaa aaaaggaata tatttttctt
    26041 cttttcatga catcgtcctc ttaagaagtg ataaacaaga tagttgattg atcaatttga
    26101 tacatattac attttcatta tatcaataaa agaaagcgtt gtcaattatt ttatttaaaa
    26161 tattgtcata ttttaaataa aatggtaaaa gagactcggt agcctcgtct ctctttatct
    26221 tactgaccta accttcttgc caattttctt ggtcttcttt aaagcgtttt agaagatcaa
    26281 ggccctctgg cgtgatgtaa ccttcttctt gggctagatg gataagctcg ctatagtttg
    26341 aaagcgtcac aagtttaaca ccagcatctg caaagttctt atctgctttt ggcaattggt
    26401 agctgaaaat cgctacaact ccaagtacat ctgctccttc tcgcttggct gctgctacag
    26461 cttcaagaac tgaaccaccc gttgaaataa ggtcttcaac cactaccatt ttttgacctt
    26521 gagctacgcg accttcgatt tgattaccag ctccgtggtc ttttggtttg ctacggatgt
    26581 aggcaaaagg caagtccatc ttatcagcaa taatggctcc gtgtggaatc cctgctgttg
    26641 cagttcctgc aatcacttct acttcaggaa aggcttcttt gatagcttcc acaaaaccat
    26701 tttcaattag ggtacgagtt tctggatagg ctagtgtcac acgattatca gtgtaaatcg
    26761 gtgacttgat accagatgcc caagtgaagg gttcctctgg tttgaggtaa acggcttgga
    26821 ttttcaagag gtggctagcg atatctttag caagtgtcat ggtattctcc ttttattttt
    26881 ctaatctagt tctttaattc cagtcctgtg tccattcatc cttgatggca tgataagctg
    26941 caacaggatc ctcagcttgg gtaatgggac gtcccactac gatatagtca ctgccgattt
    27001 gataggcatc agcaggtgtc atgactcgtt tttgatctcc aactgcaaca ccagctggac
    27061 gaatccctgg tgtcagacag ataaaatctg gattggtagc ctgcttgatg acttgtactt
    27121 cctgagccga gcaaacaaca ccatccaagc cagcttcagc tgtcttcttg gcatagtgaa
    27181 tcacagactc ttgcagactg gtttggatat tttgaaactc ctgcatctga gcttctgacg
    27241 ttgatgtgag ctgcgttaca gcgatcaatt tggcttgact cccaagacct tcacgcgccg
    27301 ccttcatcat ctctacacca ccagccgcat ggacattagt catatcgaca ccaagctgag
    27361 acaagacctt catggctgac ttgactgtat taggaatgtc atgaagtttg agatccaaaa
    27421 agacactatg acccaaacct tttaagtagg acacaatctc aggccccgct gcgtaataaa
    27481 gctccatccc taccttgaga taaaggcttt cttctgctgg gaaaagagct aaaaattcct
    27541 tgaccgcctc aaaactagga aaatcaagag caatgattgg acgatgttct cgcataggtt
    27601 tctcctttta cctttactag tgagagagtc tggccaacag aagccacgaa aaaaggaacc
    27661 tgccaacagc aaggttccac gaaaaatggg tagtctccac cctttcaccg tctaacctta
    27721 gctgcctctc tggactgctt taaaggtttt ctattattct attatttcta ctagcacttg
    27781 tcaagtaaaa acatggtcac taaagaacta taagagaaaa ggtaaaccta gcgacgcgat
    27841 gagcgctggg tcgtttgatt tcgattgctc tcttcctctt gttttttctg ttcttcttcc
    27901 tgttttttct cggcttcctt ggcctcttgt ttggcttttt cctcagcctc cataattaat
    27961 ttatccgcca cagtgtaact gtaaattcca gcttccatgt cgaccacact cggttctgac
    28021 aattgtggct taatcttact gtaatatggc agtttcttac tcatttcaga tagaggaacc
    28081 aagacttcgt ccgaatcatt catggtcaat cgaattaaat cggatgtcac cttgcttggg
    28141 gctaattcca ccttttggat agccgccttg agttctgggc taatttgagc aagttctgag
    28201 acaaaaacct tgatttgttc actatcatta aagagaactg ataaataagt ttctggtaaa
    28261 ctgttcagac tcacagaact agtctcaagc tgaccactgg aaagaatagg ataatgattt
    28321 tcaccagaaa tatagtaggc cacaatatca tattccttga ccttaatagt gaacttagtt
    28381 ggaaattgat agacaagttg agctgattca acccaatagt tagacttaat ctgcttttca
    28441 tattttgcct tgtctagcag aaggttaatc gtataatccg aatcctgaat gcctgaagcc
    28501 tgtcgaatat catcagctgt agtttgcacc gttccctcaa cacgaatatc tttcatggtc
    28561 gcataaggac tgagcaagta ggcagagaca atcaataaaa gcagacttgg aaataaaatc
    28621 gtgaaggctc gcaagatatg gataccagga atctttgctt tggctggttt ttcctttgta
    28681 gcctttttag aaagcttttt atcctgttcc tccttctctt tagactctgg ttcttctttc
    28741 tcttctttct ctttgtcagc ctctgaggat gctacttttt cttcagactc ttccttagct
    28801 gattctgaat cttcctggtc tgtttcactc tcctggtcct gtttgtcctc tgacttctca
    28861 gattcttctc ccattcgagc ttgtctttcc ttttccttct cctcagctag agccgcctct
    28921 tcttcagcct tcttttttag atattcttgg tttcgtttct gccattctga taactctttc
    28981 aattcttcga ggatttcttt gtcctcattt ttcttatctt ttgacattta ctttccttat
    29041 gataaatctt ttttcaacaa ttgataaaaa tctgctagag atttcaattc cttagaagcc
    29101 ttcatcttag cttggtaatc ttccttgtga cttagtaagt gagaaagctt ctcttccaaa
    29161 ctatccaagg tcaaatcgct ttcttgaagg tcttctgcat agcctttttt tacaaagtaa
    29221 gctgcatttt caagctggtc accacgacta gcttcacgac caagcggcac aatgacatgc
    29281 aattttgcta tcgccaagag ctcaaaaatc gtattggcac cacctcgtgt cacaacaata
    29341 tcagccaatt ccatcaaggg ttgatagaga tcggtcacat agtcaacacg aaaaagattt
    29401 tggctcaact cattcaggct agaatctcca gttagattga taatattgta gcgctctgtt
    29461 agttctttct tatggtctgt caccaattgg ttaaagacac gagcacctgc agaaccgcca
    29521 acaaacaata cagtcggcaa tttatgatta aagtggcttt gaatatccac caattcatct
    29581 ggttctggat ttttttgatc tgaaaccttg gtcactgctc ccacatgctc aaccttagac
    29641 aaactcgaag cttgctcaaa ggttgaatac atcttagtcg caaatttata ggcgatttta
    29701 ttggccaagc ccatagacag gtcagattcg tgaataaaga caggcactcc tgacacacgc
    29761 gcagcgataa caggcggtac tgagacaaag cccccctttg aaaaaagggt ctgtggacga
    29821 agtcgcaaca tgataaagag cgattggacg attccccagc aaactttgaa gacgtccagc
    29881 atattttgcc aagagaaata gcgacgcaat tttccagtcg caatagaatg gaaggtgaca
    29941 tccaaacctg acttaaggat ttcttggtgt tcgataccac gcttgtcccc gatatagtgg
    30001 acttcccaac catcttcgat gaacttgggc attaacaaaa gattgagggt aacgtgtcca
    30061 accgtccccc cacctgtaaa gacaattttt ttcatattat tcttttaact ccgctactgt
    30121 gtcgataaag aggtcgccac gtacttcaaa gttagcatac atatcccagc tggcattggc
    30181 aggactaaga agaaccacat ctccttgagt cgcaagctca taggccttgc gggtcgcatc
    30241 tgcaatatct gtcgcctcca cataagcgac accagccttg tctgctgccc gtttgacacg
    30301 ttctgcagat tgacccagga tgaccatctt cttgagtcca gtaatgtctg gcaccaattc
    30361 gtcaaactca ttgccacggt ccaaaccacc tgcaatcaag acgaccttgc tgttatcaaa
    30421 tcctgacaag gctttttgag tagccaagat attagttgat ttactgtcgt tatagaattt
    30481 aacacccttg atgtcatcca caaactggag acggtgtttg acaccaccga aggctgaaag
    30541 agtttccttg atggtttgat tgtccacacc acgaagcttg gctacagcaa tagtcgcaag
    30601 ggcattttcc acattgtggc tacctggaac accgatttca ttcgctgcca tgactacttc
    30661 accacggaag tagagttgac catcttccag ataagctcca tcaacctttt caagtgttga
    30721 aaatggtaca acagtggctt ctgtcttgga agtcaagtct tttgccaagt cttgattaaa
    30781 gttcaagaca aggaaatcag ctgctgtcat cttgttctgg atattccact tggctgctac
    30841 atattccgaa aatgacccat ggtagtcgat atgagttggc atgaggttgg taataaccgc
    30901 aatctctgga tggaattctt gaacacccat gagttggaaa gaagaaagtt ccataacaag
    30961 cgtgtcctta tctgatgcta tttgagcaac ctgactagct ggatagccga tattccctga
    31021 taaaagacca tgttgcccag cagcagtcaa aacttcccca atcatagtcg ttgtggttgt
    31081 cttaccgttc gatcctgtga taccaataat cggtgcttct gaaatcaaat aagccaattc
    31141 cacctcagtc aagactggaa ttcccttggc caaagccttt tcaatcatgg gattgttgta
    31201 ggggatacct ggatttttca ccataagggc aaactcttca tccaagagtt ccaaaggatg
    31261 gccacctgta atgaccttga tcccttcttc cagcaaactt tgggcagctg gattgtcctc
    31321 gaaaggtttc ccatcattta ctgtcacaat ggcacctagc ttgtccaaca aacgagctgc
    31381 agattcacca gacttggcca aacctaaaac aaggactttc ttatttttaa attgatctat
    31441 tactttcatg tctcgaactc catttctact cctactattt taccattttt atggaaataa
    31501 aaaagccaca aagtgtgttt gtgactcttt cttctaactg aatcttacca tatcatctat
    31561 gtgataaatc ggtaactcga atgacctgat ccacttgttc ccaaatcaga ggattgtggg
    31621 tcgcaataat aatggtccga ttcggatttt ttaaagattc taggatggaa agtaattcct
    31681 cagagttttt ggggtctaag gaagtggttg gttcatctgc gaggatcaaa ggtggatcct
    31741 ttaaaattat cttcgctaat gcaacacgtt gtgcttctcc tcctgataac tcaaatatag
    31801 gttgcttcaa atccaaataa gagaggttta cacagtttag agcttgtttc atcaaagaga
    31861 ttttctcttt ttccttcaac tttttaccaa ctaaacccag attgagattc tctttgacgg
    31921 tttggctttc aattaagcca aaatcttgaa ataagtatcc caagtaatct ctaaagaaaa
    31981 cagaaggctt gatgtcctta agagaagtac catcatagat gatttgtcct ttgtcatatg
    32041 gctctaatcg tccaatcata ttcaagaatg ttgtcttacc acagcccctt gtaccgatta
    32101 aggcataaat tttcccacct tcaaaatgaa gatttgtatc tgaaaatagc tgacggcttc
    32161 caaatttttt agatatattc tttagttcaa tcatcctatt ttcctttcat aattgtcata
    32221 gaaacacgag attctttctg cgcttgacgg taaagcgtca gaactgcact agctagaaag
    32281 actaataaag tgagcaagcc aatcaccaag tctcgactgc ttaaaataaa gagactagca
    32341 ccaaatacaa aactggcaaa ttgactaacc atatactgag catgtgtttc aaaaaatcgt
    32401 aaacctgaaa ttcgtttaat caaaatatct cggcggaatt gctcgaaata tagaagattg
    32461 acagaataaa atagtaacaa ggaactagct atccccacaa tagctcctaa gattaaagtt
    32521 gctgtttcag tttgaacttc attataacga gttagataaa cacttcttcc ttctttaaga
    32581 taggatactt gctcataaat tccagctttc ttcaagagtt ctagcccact ctcatatcct
    32641 ttgataaaga gttgttttcc agcattgata gaccaactag ataaggatat aaaactatca
    32701 cctgtagaag tcggcgtgaa taccactaaa atcggatcag tcaaatactg agtagatacg
    32761 ggattctcac cgttattata aacaaaccgc ttttctccca ttgaaagata actaacgtgc
    32821 gctttcatct cataatccaa aggagcactt gcctcctcac cagattttcc ataataactc
    32881 aatctttctt caaaaacttt cttaagttct gcttctcgag agcgcaaatg ttctgggagc
    32941 aagaggataa actcaccttt ttggagatgg gctaacttct gtttggtctc agcatctacc
    33001 acgacctttt ccttgtccaa ataactggga ctaacataga gcgtattagc atctggacta
    33061 taggtatcca gtgtctctcc ctgttcattt tttccttgtg gattggcaaa atggagcaga
    33121 ttatccttta cataaagagc ttgttcttct tcgattgctt ccttggcaaa ggcataccac
    33181 ttgctctgat tttctgtatc ttttcctcta tcacctaagc caaaagaaat ttgataatag
    33241 tctgctctat cctgccatgc ttgttttgaa atttcaagtt ctttcaatcg ttggtaagac
    33301 atcaaacctg tcttaacagc gtagcctact gtaaagacag caaccaactg acacaatagg
    33361 gttaaaatca tcaggcgttt aaggggtaat ctccccttaa taacgggaac taatgctttg
    33421 taactcaaac tcattagata aagaagcatt agtaaaattg aaatcgccaa taaaaacaac
    33481 agatagaaac taatcccaaa accataggtg gctaacaaga taggataaaa caaaccttga
    33541 ctaaaaagaa cgactccccc acctaggaag gaaaggaggg ctgatagaag gagccatttg
    33601 atatcagtag ataaagaatg ccccatgatg gataagagag tctgaccaga aaagagtttt
    33661 atacctgctg ctctcatttc cttaatccga gtgataatca ctaaagcaaa gaaagataag
    33721 ccaaatattg ctaaactaat taaaataagg ggatttagta atattcgaaa agcaagaaaa
    33781 tagggcggta tctttcggtc agcacttgct ttataaccca aatctcctaa tttatcggca
    33841 agcttttctt tcgtcaagga gcctgacaaa aggagataac tatttagcgg atcactacgt
    33901 tcacgacttt cttggctagc ttcttggaat tcttttggta aagttccctg accataagtt
    33961 gcataagtaa agtgagtcgt cccatcctta ctcggctcta caattcttct agctattaaa
    34021 ctctgttctg agtttgcaaa attctccaat tcctgttcaa atacctcacg cgtcggttcc
    34081 tgagtatctt ttttgacacg aagtaaagaa acggaatcat agcttgcata taaatattgt
    34141 ggcgcacgta agacaataat ccaagcaagg aagaagctga gaaaaaagtt gataataata
    34201 tgaatagttt cttcatagta gactccttgt aaacaaaatt ccccctgtaa tttcttacaa
    34261 ggggaacgat ttaaatcaat gaacgattag tcataatcac agtaaaatgc tacttgttct
    34321 ccccatttag tccaaatcca tgcataggag gtttttccag cagaagcttc tcctttatct
    34381 gaatgactat tccatctact tacaagagaa gaataatgat attttttacc atgatagtaa
    34441 tttgaaaaag cccaaccacc tcctgaacct tctccatacg tccatactcc tccatctgga
    34501 tattgtacag cagctgatgc agctcccaat aatgtaaaac ttgaaataag agctagagca
    34561 agtaatctat gttttttcgt tttcatttta ttttttcttt caaaaaaagc acaccttgag
    34621 caacaatgca acaaaataaa ccctcctctc tcttttgttg aaaccgcttt cttatgtgat
    34681 aaaaatatca tttttattat ttgttgtcaa ggaaaaaatc gaatttttta gatattttac
    34741 tacattgcct ctatgaataa tattatatag tagttttatt ttaaaataat atgcaaccag
    34801 tactaaccaa atataaaata gatgccatta acgaatttta ttcaagtttt tcccattcat
    34861 actatccaag caaaagagat gatgttatag tacaaaacaa ttcaaactat tgtaaaattc
    34921 ctagcaaaaa agagagccaa aactctcttt tttatcttct tttacttttt ttgactggca
    34981 tgagtgtaat gtctctaaca ctaaagtaag ctaggatcaa catggctatt gctaggaata
    35041 tttctgttgg taattgaaaa attttcagaa aagatagaac caataaaatc aagagtgcca
    35101 ctaaaataca taccatagcg acgatattga ctgtcccttt aatgctttct ggtgtcgcaa
    35161 atacatagag taggagcagt aaaattccta ggactaaata gaccatcttt ctctctttct
    35221 agctcttatt cagctgattt tttcttcttg ttagctttct cacgctctgc tttgttaagg
    35281 atttgtttac gcaaacggat agactcaggc gttacttcca tgtactcatc gtcgttcaag
    35341 aactcaagag actcttcaag tgtcaagata cgaggcgtct tgataacagc tgtttggtcc
    35401 ttagtagctg aacgaacgtt ggtcatttgt tttgccttcg tgatgttaac tgtcaagtca
    35461 ttttcacgag agttttcacc gatgatcatt ccttcataaa cctcagtacc tgggttgaca
    35521 aagatcgtac cacgttcttc gatagacatg attgagtaag ttgtagcctt accagcatcg
    35581 atagaaacaa gggcaccacg gtgacgtcca ccaatttccc ctggaatcaa tggcaagtat
    35641 tggtcgaagg tatggttcat gataccgtaa ccacgagtca ttgacaagaa ctcagttgag
    35701 tatccaatca aaccacgcgc tggaacaagg aagaccaaac gagtttgacc attaccagtt
    35761 gaaatcatat ccaacatttc acctttacgt tcagaaaggc tttggataac agacccttgg
    35821 tattcttctg gagtgtcgat ttgtacacgt tcaaatggtt cacatttaac accgtcgatt
    35881 tcttttacga taacttctgg acgagatact tgaagttcat agccctcacg acgcattgtt
    35941 tcgataagga ttgacaagtg caattctcca cgtcctgaaa cagtccattt atctggtgaa
    36001 tcagttgggt caacacgaag ggaaacgtct gtttgcaatt ctgcctgcaa gcgttcttcc
    36061 accttacgag aagttaccca tttaccttct ttaccagcaa atggtgagtt gttgaccaag
    36121 aaagtcattt gaagagttgg ctcatcgatg tgtaggattg gaagagcttc tactgcatct
    36181 gtcggagtga tggtttcacc gacaaagatg tcttccatac ctgaaacggc aatcaagtca
    36241 cccgctttgg cttcttggat ttcacgacgt tccaaaccaa agaaaccgaa gagttttgta
    36301 acacggaagt ttttagttgt accgtcaagt ttagaaaggg taacttggtc cccaacctta
    36361 actgtaccac ggaagacacg accgataccg atacgtccaa cgaagtcatt gtagtccaaa
    36421 agtgacactt ggaactgcaa aggctcatct gagttatcta ctggagctgg gatatggtcg
    36481 ataatcgtgt caaagattgg tgccatagtc gcttcttggt cagctggatc atctgacaat
    36541 gaagaagttc cgttgatcgc tgaagcataa accactggga aatcaagctg gtcgtcatct
    36601 gcaccaagct cgatgaaaag ttccaagact tcatccacta cttctgctgg acgagctgat
    36661 ggcttatcga ttttgttaac aaccacgatt gggacaaggt cttgttccaa ggcttttttc
    36721 aatacgaaac gagtttgtgg catggttcct tcataggcat ctacgaccaa gacaacaccg
    36781 tcaaccattt tcatgatacg ctcaacttct ccaccaaagt ccgcgtgtcc tggtgtgtcc
    36841 ataatgttga tacgagttcc gttgtaagca acggcagtgt ttttagcaag gatggtaatc
    36901 ccacgctctt tttcgatatc gtttgagtcc atagcacgct ctgccaattc agtacgtgca
    36961 tcaagcgttt ctgattgttt caataattcg tcaaccaggg ttgttttacc gtggtcaacg
    37021 tgggcgataa tcgcaatgtt acggatatct tctcttaatt ttgtcatgat ttcctctata
    37081 atattcaaaa tttattttct aactgaacga ttataccata atttcaaata aataacataa
    37141 ctcaagcaag tgtaaatgtt ttcactccgc ttttcttttc acgtcaagcc ttttcaaagc
    37201 aagcgactta tgataagata ggcacagtat gcgtttagat aatttattag cccaagaaaa
    37261 agttagccga aaggccatga agcaagcact cctcagaggg gaaattctag tcgatggttg
    37321 cccagcccgc tccctagctc aaaatatcga tacaggacta caagaactcc tttttcagga
    37381 tcgaatcatt caaggctatg aacacaccta tcttatgctt cataaacctg ctggtgtcgt
    37441 tacagccaac aaagacaagg aacttccgac cgtcatggac ttgcttccat ctgacatcca
    37501 gtctgacaag ctctatgccg tcggccgact ggaccgagat acaacaggac tccttctctt
    37561 gaccgataac ggtcccttgg gcttccaact cctccatccc caatatcatg tcgataagac
    37621 ttatcaagtt gaggttaatg gacttctaac acctgaccat atccaaacct ttcaaaaagg
    37681 aattgtcttt ttagatggca ctatctgtaa acctgcaaga ctagagattc tatcagcaag
    37741 tccttccctc agtcaagcct ctatcaccat ttcagaagga aaatttcatc aaatcaagaa
    37801 aatgttcctc tcggttggtg ttaaggtgac taacctcaag agaatccaat ttggggactt
    37861 cacattgaac ccagatttag cagaaggtaa ctaccaccct ttgaaccaaa aagagttaca
    37921 aatcattaaa aactatttag agatgagtcg ataaaacaaa aaaagcttta aaactaaagc
    37981 ttttttcttt tatttaccga aaaattaagg cgattgctac aatccagtta actacagaaa
    38041 tcacaattcc taagatatta agaatctttt ctattttata gtctaattgt gactcttttt
    38101 ggtatgaaat agccaagacc aatcctatga tacccaaaat caggcctaca attggaaata
    38161 acaaaccaag aataatcgac aagataccca caaaaagtgg atttttcttc ttttctttca
    38221 tgttctaaga actctttaaa ttttatacaa attaattata ctataaaaca atagcttcat
    38281 tctatcattc gactaatttg gaaataaggt tagctagtct tcactttccc tttccaagaa
    38341 tccaagccat aagaaaggat ataaatctca gaaaaacctt gttttttcaa gtaaagagct
    38401 gcatttgtaa ctcgttgcgc acgttggttt tcgtagagaa ggacaggttt atctttacga
    38461 agggctgcaa gactagtttt caactgactt gaaggaatat tgcgtgcacc aaggatatgt
    38521 tttctgtgga attctgctgg gtcgcgcaaa tcaatcaatt gacccgtacg aatcaaggct
    38581 tcaaactcct cattgtccac aattttagcc gcacggcgaa tacgaagata gttaaagccc
    38641 atccacgcca acattgctag tataagtgcc cacaaaatcc aagtaaccat tagttctttt
    38701 ctccattttt ctcaatataa tccaattcta ccttgtgctc tctgcgaaga actgcttctg
    38761 cctctagata gtctaattta tccatcaacc ctgcatcgta aatccgagat agttccaact
    38821 tcatcagttc aatatcatat aagcgttttc ccatgtaaac aataatacca aatcgtttga
    38881 ggaattgctg cacatcatag aatgttttca taagactcat tctagcaaaa ttttgtgttt
    38941 ttttcaagaa gagactcaca caatgctcct tattttccta tcttctttag cgattctaag
    39001 gcaagtatgg tacaataaaa acatgagaat tcaacaatta cattatatta tcaaaatcgt
    39061 cgaaactggc tccatgaatg aggcagccaa gcaactcttt atcactcagc caagtctctc
    39121 caatgcagtg cgagatttgg aaaatgaaat gggcattgag atctttatcc gcaatcccaa
    39181 gggaatcacc ttgacccgtg atgggatgga gtttctctct tatgcccgtc aggttgtcga
    39241 gcagacccag cttctggagg aacgctataa aaatcctgtc gcccaccgcg aactctttag
    39301 cgtttcgtct caacactatg cctttgtggt caatgccttt gtctctttgc tcaagaaaag
    39361 cgatatggag aaatacgaac tcttccttcg tgaaactcgg acttgggaga ttatcgacga
    39421 cgtcaagaac ttccgcagtg aggtcggggt cctcttctta aacagttaca accgtgatgt
    39481 tttaaccaag atgctggatg acaatcacct gctagcccac catctcttca cagcgcaacc
    39541 gcatatcttt gtcagcaaga ccaaccctct ggcaaagaaa gacaaggtga aactgtctga
    39601 tttggagaat ttcccttacc tcagctatga ccaagggacg cacaactcct tctacttttc
    39661 agaagagatt ctttctcagg aacaccacaa gaaatccatt gtggtcagtg accgtgccac
    39721 cctctttaat ctcttgattg gtttggatgg ttataccatt gcgacaggga ttttgaacag
    39781 caacctaaac ggagacaata tcgtttctat cccactggat attgatgacc cgattgagct
    39841 ggtctatatc cagcatgaga aaaccagcct atctaagatg ggcgaacgct ttatcgacta
    39901 tctactagaa gaagttcagt ttgatagttg agaaatgata agaaccaata tgtaggctag
    39961 caacaacctg cacattggtt ctttttactt ataattaaaa gtttcccctg ccaacttatc
    40021 agctagcttg ggaaagagag tataaaactt atgggctagg ttcaacaaaa tcgggagatt
    40081 gagttctcgt ttatttttgc ctataatctt gacaatcttt ttagccactg catctgcctc
    40141 taagaggaag cggtcaaccg atttaagata agttccatct gggtcagctt ggtcaaaaaa
    40201 tcctgttcgg attggacctg gattgactgt tgtcacatag actccatagg gcataagttc
    40261 gagtcgcaga gcatttgaaa aaccaatagc cgcaaacttg gtcgctgagt aaagactaga
    40321 cttgccagta gctattagac ctgccatgct gacgatgttg atgatatgcc ctttgctgct
    40381 ttccttcata cgagccgcaa ggtgacgaga cagattcatc agggcaaagg tattgacctc
    40441 aaacatctgg tgaatatctt tatcagcaat ctggtcaaat ccctcaaaaa tcccgtaacc
    40501 agcgttgtta atcaagacat caatcttgcc atagcggaga taaagatcag ttaccagagc
    40561 ttctagggct gaatcgtcgg taatatcaat ttcaatcaat tctgcatggg aataatttcc
    40621 gtagagttgg gctaattttt ccttatttct accaagcaag atgagttggt cattgggcag
    40681 gagtttgacc atttcttgag ctagaccacc gctagctccg gtaatgagaa tagtaggcat
    40741 acttatcctt tctgtgactg ctagatttcc acttcttcca agtctttgac cacatggaca
    40801 ttttcaaaaa ttgtggcagc gtccttcttg agtttgctta tatcttttga gaggaaacgg
    40861 gcactgatat ggttgagtag gaggcgtttg gcacctgctt ctaccgctac ttgtgcagct
    40921 tgcatattag ttgagtgacc atggttacga gcaatttttt catcaccctt gccataagtg
    40981 gactcatgaa ctaggacatc tgcattgaca gccagacgca cactggcatc cgtttttcga
    41041 gtgtctccta aaatagtgat aatcttacct ggacgtggcg ctgagatata gtctgctgcc
    41101 ttgatttcag ttccgtcttc caaaacaaga tcctggccgt ttttgatttt accaaaaagc
    41161 gggccgaacg gaacaccagc agccttgagt ttttcagcat ccagcgtccc ttctagatcc
    41221 ttttgcatga cacgatagcc aacacagaaa atagtgtggt ccagctcctc tgcatacaca
    41281 gtgaatttat cggtttcaag aattttaccc agagaatctt ggtcaaactc atggaaatga
    41341 atgcggtagg gcagacgaga acctgacaca cgaaggctgg ttaagacaaa tgacttgatt
    41401 ccttgaggtc cgtagatttc caaatctgtc tgctcttcat tggcctgaaa ggcacggcta
    41461 gaaaggaaac ctggcaaacc aaaaatgtgg tctccatgca gatgggtaat aaagattttg
    41521 ctgaccttac gtggtcgaat tgtggtttcc agaatgcgat tttgcgtacc ttctccacag
    41581 tcaaagagcc aaacttcgtt aatctcgtcc aaaagtttca gggcgagact tgaaacgttg
    41641 cgggctttag agggctgacc agcccccgtt cctaaaaatt gaatatccat tcgatacttt
    41701 ctaattaatc aatatataac atggctgtgc ggttttccga tcggaaatag cgtttgccag
    41761 aaaaagcagc agcttcttgc aataaatcct cttggctgta gcctttgaga cgttttcgac
    41821 catcagccaa tctttccaaa tcagtcaaag ctgtgagact ttctaggctg acaacttcct
    41881 cgtccttgac aggtttcatg taaatcttac cagactcttc aaagactaat tgatgggaga
    41941 aaatttgcgc aatttcaaag agcaagtcat ccgagatttt ctcctcattt tcagagaaaa
    42001 tccgaccaag gccgtcactc tcataacaaa aaccaaagga tttaccagac agattaagcc
    42061 gaataaaagg cttattttct agggtgaaac ttggctcagt attgtaaaga ttcagttcct
    42121 gactgagttc tgcaaaataa tccgtcgcag cctgaggact ttttttctga tagagttctg
    42181 caaagtaggc attaacaaca cttggcggag gtgtaataag tgttaactgc tcctgctctg
    42241 ttttaccagc tagaagctga tccagataga ccttgtccag acttgtataa cctccatact
    42301 ttagagccaa agttttaata tcagtcataa aattcttcta acctccattt atttttctcg
    42361 gaaatgtagc ctgtaatcac ttcgccgtct tcctgataat cacgttcttc cagaattgca
    42421 acactctcta aatcatgaat cttgtaggac tttgaaaaag gcactcgcag ggtaaatgct
    42481 tcaaaaattt ccttaatctt atctagcaat aatgcttgca agttttcacg actgtcctca
    42541 gacttggcag aaatgagggt atatggcgtt tgggtaggcg tgaaatcctc caccaaatcc
    42601 gctttattat aaagcgtcaa gtgaggaata tcttccatgt ccaggtcttt catgatggag
    42661 agaaccgttt tttcatgctc ctcgtggtaa ggattgctag catcgataac atgaaccaga
    42721 aggtccacat gcttgctttc ttccaaggtt gacttgaaac tggacaccaa ctctgtcggc
    42781 aaatcttgga taaagccaac ggtatctgtc aaagttactt ggagattgcc tcccagatga
    42841 atactcttgg ttgtcgcatc cagagtcgca aagagctcat ctgcttcata ctgggtctta
    42901 ctggtcaaga tgttcatgat agttgatttc ccagcattag tataaccaat caaaccaatc
    42961 ttaaaagtgc tagactccaa acgtttttct ctgacagtcg cacgattttt ctcaaccacc
    43021 ttgagctggc gctcgatatc cgtgatttga ttgcgaacgc tacgacggtt cagctccagt
    43081 tggctttcac caggaccacg ggaaccaatt ccccctgcct gacggctgag cataatcccc
    43141 tgaccaacca agcgaggcaa gaggtatttg agttgggcta ggtggacttg gagcttccct
    43201 tcatggcttc gagcccgcat ggcaaagata tccaaaatca actgcatacg gtcaatgacc
    43261 ttaacaccga gaacttcctc tagattgaca ttctgccttg gggtcagacg gttgttgacg
    43321 atgacagtag tgatttcttc tgcatccacc ataagcgcaa tctcttccaa cttaccagag
    43381 ccgacgaagg tcttggaatc atatttttca cgtttttgtc tgtagctatc tacaacgact
    43441 gcccctgccg ttttcgctaa actagccaat tcttccatgg agaggtcaaa actgtccata
    43501 ccctgcaatt ccacaccaat cagcaggact cgctcctctt ttttctccgt ttcaatcatc
    43561 taaaaactcc tctatctggc ttaaaatgcg gtcttgtaca ccagattctc caatctgata
    43621 aaaggtgacc tgcatgcgat tacggaacca ggtcagctga cgcttggcaa aacgacgggt
    43681 cgcctgttta agactctcac gagcttcctc aaaggtctgc tctccacgga aataaggaaa
    43741 gagttcctta tagccaattc ctttagcagc ctgtacatta ggggaatggt caaacagcca
    43801 cttggcctca tccaaaagcc cagcctcaaa catcaaatcc actcggtggt tgatacgctc
    43861 ataaagttga ctacgttcat catccaagca gataatcagc ggttcataca aaatctcttg
    43921 attttccaaa tcctgaccaa aatgggcaat ttctaaggca cgcatagcac gacgacgatt
    43981 aaactgggga atctcaaggc ctgcttgctc caccaaatgg gctaattcct catctgaata
    44041 tggctccaaa ctagctcgat aagctaaaat ctcctcatga ggagtctccc cacctaggtg
    44101 gtaaccttct agcaagctct ggatataaag tccagtccca ccggcgataa tggctagctt
    44161 gccacggctg tgaatatcct caatagtcat cttagcttct gaaacaaaat caaaagccga
    44221 gtaagactcg gttatctctc taacatcgat taaatgatga ggaacagctg cctgctcttc
    44281 tggactagcc ttggccgtcc caatatcaag tcctcgatag acttgttggc tatctccact
    44341 aaccacttcg ccattaaagc actttgcaac ttcaatagct agagccgtct ttccaacagc
    44401 agtcggtcca acaatcacaa ttatttttgt tttcatcttt tttccttgaa aaattcccat
    44461 tttttcgtta ctattattat aacacaaaaa ggtcagtcag aaaaatgtga cctcttgagg
    44521 aggctggctg attgagaata taaaggagga agactcatgg ctaaaggatt cgctaaaggt
    44581 cttgtaacag gtgtcgcagg aactgtcgct gccgttgcag gtgcagtata cgcatttaaa
    44641 aagaaagtaa tcgaaccaga agagcaaaaa gcagctttca tcgaagaaaa ccgtaaaaaa
    44701 gcagctcgtc gccgcgtatc acgttaagaa acaaaagaag ttgggattat tctcaacttc
    44761 ttttttctat tcttttaaat agctaggtca aacttcaact gtggcttaac agggtcataa
    44821 tccaccaact caaaatcttc tgctttgata tcaaagaaat tagtcccatc aggaacattt
    44881 aaaaccaagc gtggttggca gtttgacggc tcccgacgga gcaattcctg agcttgttca
    44941 aattgattat catagatatg gaggttgttg atgaagtaga agaacttccc aaccttccag
    45001 ccaaaatgtt tggcaatcat catctgcaaa gccacatact gcatagcgtt gatgtggtgg
    45061 gccaccagca tatcattgga gcgctgggtc aaggtcgcat ccagatagat ttccccatca
    45121 acacgccgga catcaaacat ggtctgaaag gcgcacggga gcagcccatc tgtttcttcg
    45181 aaagcttggt aatcccagag cgaaataata ttgcggcggt tccaaggatt ggtttccaac
    45241 tgtttgagaa gcttattgat aatgtcgtgt ttcttaacaa cggcaccata gcgctcacca
    45301 atggttcccg tgtctccaac ttcccagtca ttccagtagt gaacattgta cttgtcatta
    45361 agcacttcta agctatttga ctggtcttgg taaatccaga gaacttcttt gatggcggat
    45421 ttgattgcaa tgggacgcaa ggttgtgata ggaaattctc ctttagataa gtcgtactcg
    45481 gaaaaggcac cagttacata cttggaatta gcaacagtcc catccttgta cttaggacga
    45541 gcctgctctg agaaaacacc ttcctttaga atgcgttcaa tattctcttt aaaaatcgta
    45601 tctgcttttg tcatttcgtt tcacctcatt cattgtccta actagtatag cataaaaaat
    45661 aaaagaggaa cattactaac tctaggttag cattttcctc tctcattatc ttattgcaat
    45721 acaagtgatg ctgctccgat aactccagcg tcatttccta gagttgcaag agctaattta
    45781 gtagatgtgc gtacttgtgg gaaactattt tcatcgtaaa ctttttgaac accttgtaga
    45841 aggaattctc ccgcagctga cacaccacca ccgatgacga ttgttgatgg gtttaggatt
    45901 gaaccaatgt tagcacaagc gattcccaag taacgtgaga agttacggta aacaatcaaa
    45961 gcaaggtcgt ctccttcttt tgcgagatca aagacagtct tagcagttac ttcttctccg
    46021 ttatcaatca agcgtttcaa ggctgcatcg ccttcgtatt catcggcata gcgacgagtc
    46081 aagttgacaa tccctgttgc tgaagcaact gtctcaaggc agcctttttt accacaagta
    46141 catgagattg gttggtcaaa gtcaacagtg atatgaccaa gttcacctgc tgcaccagca
    46201 acaccatgaa gcaatttgcc ttctgcgaca ataccaccac caacaccagt accgagtgtc
    46261 ataaagacaa cgtctggttg gttatctcca gcacccatcc agcgctcacc aagagctgct
    46321 acgttggcat cattatcgat gaaaaatgga atgcccaaag ctttttcaat cttttgtttg
    46381 attggttgaa gggttttcca gttcaagttg taggcaccga taacagtccc tttttcacgg
    46441 tcaaccacac ctggtgatcc cattccaata ccttggaagt ccgctgctgc caatccaagc
    46501 aagtccaaac gatgttgaat agactcaatc atatcatcaa cgatatgact tccctcatcc
    46561 aaaatgttgg tcttgattga ccattttcct tggatttctc ctgctgttgt taagattgca
    46621 aatttgatag aagttccacc aaggtcaatc ccaataatct tttgactcat catattctcc
    46681 tttttcattg tgtagtaatt gaaaatgctt acagtactag tatatcaaaa tccattcact
    46741 tatgcaaagg tttgcgtaaa tgaattataa ggaatttttt cgcctaccag tatctgttaa
    46801 ccatctaaat gatcaatggc atattgagct gcatcatttg aaaatttatc aaaatcagaa
    46861 gttaattgtc tatacatacg tttttttgac atgtggaata acgaattata actttttgcc
    46921 tttcctaaag cagcagaata ttcactattg ctatcattac tagaagaagc tgtacttgct
    46981 tgaccttcct tccaaactcc gtcagcgcca acataataac gtccaccatc aacccattca
    47041 ttgacagcca ttttaccatc cgattttaga tagtaatttc cttgccatgt actgtaagca
    47101 taagaaccat ctgatgtcaa ataataccaa gcttgatagg tggcatcata aacccattca
    47161 cctttagcca ttttaccatc tgatttcaaa tagtagtttc cttgccatgc attgcgagca
    47221 tatgaaccat ctgatttcaa gtaataccat gcttggtaag aagaatcata aacccactca
    47281 ccttgtgcca ttttaccgtt tgatttaagg taataagctc cttgccatgt actgtaagca
    47341 taagaaccat ctgatgtcaa ataataccaa gcttgataag aagagtcata aatccattca
    47401 ctctgtgcca ttttaccatc agctttcagg taatagtttc cctgccatgc attttcacta
    47461 gatttccatt tccatcaaaa tagtaccaag aaccgtcctg cttaacccaa ccacttgctg
    47521 tggatgaagt tgtatcagtt tgagttgtag ccgcttgttt tgctttaaaa gcacccttag
    47581 gagcattttt caattcgcaa gccacaccat catgatcgcg gtctaacttg gcagaataac
    47641 caggttctcc ctcatgaaga tccgaatatc cattagccca agcttcctta caactcgaaa
    47701 aatgaatgtt ttcctctgct aacacaggtt gtgaaaacaa ggctaaaatt ggcaacgtca
    47761 ccagactcat ttttaaaaat agacgtttat tcatttcttt ctcccataat tatgatatcg
    47821 atttcaatat tattttatca aacttatttc agcaattcaa ggaaacttct ggtcttttct
    47881 acaaactcct ttggttttgc cttattcaag acatgagggc catctgggat aatctgaaat
    47941 tgggattctg atattagttt atgaagactt ctcatagaac taagattcgg tttatctttg
    48001 ataccacaaa ttagtaaggt tggataaggg caagttcctg ctatatccgt taaatcaagt
    48061 gtcttcaact cctcagaaac tccgaccata agagccttgt ctgctccctg tttttcaaat
    48121 actcttttgg gaagtagttt aaaaatcagc aactgaagat aaaataggat attccctgct
    48181 aatttaagcg ggcatcctga cagaatcaaa gctcgaagat ttggtaaatc gtaactggaa
    48241 agttctagtg ccagggcagc acctaaggac aatccaatca aaacaaaagg ttctgtctct
    48301 tgagctaggt gctgataaac tcgctcctta gcttgttgat agttactaac tccagaagga
    48361 aataactcga tagcctcaga aggataatct gtcagtagat tccgaacttc tttccaagac
    48421 tctgctgact gccctaaccc atgcaaaaat atcagtttca tctagttctc ctcaaggctt
    48481 agttcataca agcctctcac tgcattacag ccgtaaatag cttctgcttg ggctaaatct
    48541 gccaaggtca agactttctc ttctacctgt cctgtttcta gcaaatactg acggtaaatt
    48601 cctggcaaga ttccaagtcg gataggcggt gtgtagagtt ttccagcgat tttcagaacc
    48661 aaatttccta tagaggtttc aagcagtttt cctgacttat tgtggtaaat cttctcttgt
    48721 tctcctaggc tcaaatgcgg tcggtgagtg gttttaaagt aggtaaagga ttgattcaaa
    48781 gcagcttcct gaaggcagac ttgggcctga caaaagcttg tactgagagg ggttaatact
    48841 tgacgattga cttctatctc tccagatttg ctaaggctga ttcgcaagcg gtaatcttga
    48901 ttagcttcac aatcctgaca ctcttcctca atcttgtgtc ccaagtcttc tgcatcaaaa
    48961 ggaaaggcaa aataacgact agcttttctc agtctttcca gatgttgatc ttcaaacagc
    49021 aggttcttct ggctgatttc ccctgtcgta atcagttgga agcgggcttg tttacgataa
    49081 agaactgctg ccttttgatg aacctctcgg tattcagatt cccatgtgct atcccaagta
    49141 atccctccgc caactccata aatggcttga cctttgtgaa gttgaatggt acgaatggcc
    49201 acattaaaaa tccgtcgtcc atttggaagc aagagaccaa tcgttccaca gtagactcca
    49261 cgcggttgag gctccaagtc cttgataatc tccattgtcg caattttcgg tgcacccgtt
    49321 atggaaccac aaggaaagag tgagcggaag atttcaacaa ggtccacatc ctctcgcaac
    49381 tgactcttga tggtcgaagt catctgccaa acagttgaat actgctctac ctggcacaga
    49441 cgctccacgt gctcgctccc aacttcagaa atacggttca tatcattgcg caagaggtcc
    49501 acaatcatca tattttcaga gcgatttttg ggatcctgtt ccaaccaact ggcctgttca
    49561 agatcttctt ggtcagttac cccacgctga gtcgtcccct tcattggtcg tgttgtcaac
    49621 tcgcgatcat tttgctcaaa aaagagctct gggctcatgg aaatcactgc catctcgtca
    49681 tgttccacat aggcattgta gcccgcctcc tgctctacca ccatacgatt gtagatggca
    49741 aaaggattgg cacttaactt ttgcttgagt tggacggtgt agttgacctg ataggtgtct
    49801 ccctgcctca agtgatggtg tatctgggca atggcctttt catagtctgc tgcagacgtc
    49861 acttcctgcc aatttgaggg caaatcaata tcctcataag tcagaggaat aggggaggtc
    49921 tctaccctat catgaacagt aaagtaaagc aggtactctc ccagtagggg atccttgtga
    49981 actgctaatt tttcctcaaa agcaggggca gcctcgtagc tgacataccc caccacataa
    50041 taaccttgct cttggtagct ttccacttgt gccagcaaat ctgccacttc tgctacattt
    50101 ctcgttttta tctctttgat aggctgggta aaggtgtatc tctcccccaa aatcctaaaa
    50161 tcaatcactg tttttctatg cataccttaa gtatagcata aaataagaaa accctcatcc
    50221 gcaaagcaga tgagagattt caattattta aagattgaag ttttaaagct atttgtttgt
    50281 tgaagaagtt tcttataaac tttttctttt acttgcacaa gtttaccatt aactttatta
    50341 gtaaccatat cttcttcaag agctttggca aataatgatt ttaattcatc atagttatga
    50401 atggtctcac catcaatagt aaaaggagta aaaccagcgt tggctttttc taacaattct
    50461 ttataatatt ctttcttaaa atcagcaaac gattggaatc tgccgtcaga tattttttgt
    50521 ataatatatt gatctccaaa ttgtttccct tctttattag cttgagattt taatttatct
    50581 gaagcatatc ctaagaatcc tttatcatag ccataatatc cccagagtct aaatgcatta
    50641 ttcttaaatg taacagcacc gggtgcaccc tcacttgtgt tacctgcata gatactattc
    50701 atcattttaa ttcctacata ggcaatacct ggatcaaata cttctggctt atatatatta
    50761 ttattcatca tacgattagt catcagatta ttatcaacta agtcattaac actgtttaat
    50821 gttattcctt tccaatcatc agagctcata tttcgaacct tgtcatattg atcttttgac
    50881 cctacagtgc gttcaatagg atcaactttc ttaaaccaca gttgatttaa tttatcatca
    50941 tgtttactaa tgactgcatc accttcaaca taatcaagaa gagacaaggt atctatatag
    51001 cctttcatat aattatcaat gtcttctctt gttttaagag tcttcggatc ggtaatatac
    51061 cactgatttc catcatttgg acgatagaaa gtcatattaa ttcccaaagc accgtattca
    51121 tcaaaccatg tacttggtac tggtgtttgt aacattcctt gtgcataggc ttctgcatga
    51181 gtccctgagc gacgtccata tcctcctaaa tagattgcac gatcattaac atgagtagtt
    51241 tcatgtgtat aaacagagat tccaaactca tcaatcatac ttaatgcaat ataatttact
    51301 tctgttcgcg tatccaaagg attatcccaa ataactgcat aagcacctgt tccattgtta
    51361 ccataatact taccaatagg tccaaacaac tctctcaatg gggcatagtc ttctttatta
    51421 tttctcttac catacttatc aggccatcca ccaggtgctt gataaccttc ccaaagagga
    51481 gttactgtat ttttgactag catattctta accttatcta aagataatct ataccaaaaa
    51541 tcaaaataat ttcgataact gtccgctgct ttttcaatct gattttttat tccatcgaag
    51601 gagctatgcc ctaacttttc tgctgttcca acagccattg cactataatt cgagattaaa
    51661 tacacatgtg atttatttag atttaataat gggagtatca tcttccgatg agtagcagat
    51721 tgtaaattat cccaaacttt atatttcgcc gctttaattt catcgacttc cgaagcacgc
    51781 tccgctatat agacattccc ttctgtagct ttcttaaacc aatcataaat atttacatct
    51841 gcagtaaata gtcgacgatt ataatttaaa aagtttacca aattttctga taaggttgtc
    51901 ttagaaataa tctcgctgaa agcatcatat gtccgatgac cttttatatt atcttctctt
    51961 gaaccgattt caattaatct atctaataca cttacatttt taccatagaa atctggtttg
    52021 aataacatta attctttaat gttaacatca ccgaatttaa caccgtagta acggtttaag
    52081 tatgttaaac ctaataatag ggcagcttta tttttctcaa ctttatcaag aatcatttgt
    52141 tgagctgcag atgaattatt cagttgatga tcttcatttt caactaattt agttactaat
    52201 atgtttaaat tagcttttac atggtcaaaa ctttcttcta aataaaggtc tttaattgca
    52261 tttactctat tagaccctgt tctgccaagt ttttggtaaa ttggattaga ttgaagctca
    52321 atagggctta agatatttac gataccgtta attaaagcac tatgatcttt ttgaacgata
    52381 ttaggtgtat atttaatacc taagtcagtt atagtgtatt ctctaacatt acttagacct
    52441 tcatcactag aagataggtt gaagtagtct tttgttccat cagcataatg aacgataatt
    52501 ttgtttgcat cacctaaatc agttacaaag ttattattgt tcataggagt aaccgataga
    52561 atctctttag tatttaactt gttgttctta tctagtttat tagcttgatt aacgataaaa
    52621 tctttgttat agaatggttg tagtttttca atattcttat aagcaagttt atagtctgca
    52681 ttataatctt gaatagactc gtaagctgct tcacgactgc taacattatt caatttgttc
    52741 aattcataag gttcacttac gaatttagca gcagtaatat tgaaactagc aacttttgca
    52801 tcagcctcag atttagataa gtgttttaat ttatttttgt ttttattatc tttaccagtt
    52861 ccatcttcag aagagctata tccaataact ccaaataaat tagacacaaa aggatttgca
    52921 tctccatcat taacatcgtt tgaaccgaat atttcctgac ctttaataac tttcgcatat
    52981 gttacattat cttgaaccgc tccatatggc caagttttag aggttattcc accaacatat
    53041 ttagtatttt taacatcaag aatacctttt gctacagagt ttgtaagttt tccttttgtc
    53101 cctgatagcc agctatgagc tttatattga acgaaaggaa caagcatact tgcatttgcg
    53161 ttatttgctg taatagtagc atcaacgtag gctctattaa caaaccccat atagttagaa
    53221 ccggcaatac ctccaactgt tgaattatta ccagcagaat ttatactacc gataaatgcc
    53281 acattttcta aagtagcgtc ttcttttatg ttatttacga acccagcaac attatttcta
    53341 gctgttatgg ttccgattgc tttgatattt tctatacgtg tcgttccact gatttcattt
    53401 gctattgcag cgatattatc ttgattagat aaatcaatat caatattttc aaaatttata
    53461 tttttaacag ttccagcctt aatcttatcg aataaaggat gttccaaatt atggatagca
    53521 tagcgtttat caccatcact caatagttta ccagtaaact ctgaagtaat ataagatttc
    53581 cctttaggaa caacgttacg agcgctcatg ctttgaccca gtttaaattc accggcagga
    53641 tttgtctgaa tggcttggat caattcttca aagtcgtaga aaataccagc ctgactagct
    53701 ttgggttttt caatatagta ggtataagtt tcttcaaagc gattatcgac attacgttgg
    53761 accaagttat ccgctactgc tattaccttg tagacttgtt ttccatcaat tgttgactct
    53821 tcaatttttt gaaccgctag tagattggtc ttatggtcat tagaggtaac ttttaaataa
    53881 taatttgaca aatcagttgg cactgaagtc aatcgagttt catcagtttc aacttggttt
    53941 tcatatttga ttaaatccgt tcttataata tcttttattt caactttttt gagatcaatt
    54001 cttagcggtt cttctttcag aacttcttcc tcatccccct caccacggtc ataaaccatc
    54061 tttgtttcaa gtttataatc cttgtagtac tgcaaatctg tcagggtagt tgtcaagtct
    54121 tctggtgaaa cattcaaggt tttgacaatc tcatcacctt ttttcagagt tagggtaata
    54181 gatttaatgg ctgccttact tggattttct aaactatact taacatctga agtacgcttc
    54241 aaatccttag attcaatttc tgtgatggtc aacactggtt tttcaaagct cttggtacca
    54301 cgtttcaaaa ttttgtcttg aggttcctca aggatttcct ctgtaacttt tggagcgtct
    54361 tctgttttaa ctcccttaat ggtattaaag gtcttggtga tttttttctg gccttctttt
    54421 ccttcttgag ctacaacttc taagcctttt ttcagttggt catcttcttt aacaacttcc
    54481 ttaaatggaa tcttttcaag actttcttct ataagagttc cttcaattgg ttttgtacca
    54541 cggattacct gttgatttac aggctccttg gtcacttccg tactggttga aagaacttgg
    54601 tcagttttaa caccttctac tgttttatag gttgtttttg taacttgact tcctttttca
    54661 ccagttcgta ggacagtttc ttcgtccgta tacttggttg gatcctcagt cgtttcagtt
    54721 ttgtagtcta ggtctgtagt cgatgtttca acaagggttc cctcagttac tttgtactca
    54781 ggtaattgtt cttgagcaag agcctcaccc acgtgacctt tttcttgcgt acctttagct
    54841 gttttagtct cctcttttga ttctactggt tcttcaacct tgatttctgg ggcttctgct
    54901 ggctgttcat cttcttgttt cggtgtctct tccgctcccc tatcttcttt aggattttct
    54961 tgttgtgatg attcttttgg tgtacttggt tgctcagctt gtgtaggttg cacgggttgt
    55021 tctgggactt ttgcttgttc tactggtggt tcaacttttg attcctcagc tggtttgtct
    55081 gatggttgac tttctggctt aactgctact ttttcctctg gttttgactc aacttctcca
    55141 cctacttctt caactggagc tggttctgct gaatcttctt tcccctcttc tactttagga
    55201 agggtgtcgt cagtaggttt tacctccgat tttggttctt cctttggact ttcttctgtt
    55261 ttaggtgctt cttcttttgg agcttcctct gtctctacta cttggttttc tgtcctagct
    55321 tgctcctgat ttgttgttga ttgaggagtc tcaacttcga ctacagtcac ctctccaggt
    55381 tttgctgagg tttcttctaa aacagtgtcc aagccaagcg ttttgaggat gtcacctgat
    55441 agataaccaa catagcgata gccatccatt tcaacaacac cctctcgact agccagcgct
    55501 agggtcgcaa ctgggtctac agcccctgca ctaggaagaa ctaccaatcc catagctcca
    55561 actagaaaga cgctagcaat tttctttctc ttgtagatta aaagcaagct cccaacagtc
    55621 agcaaaccaa aagctgtcaa aacagatgct tctgtccctg tttgaggaaa ctgatctttt
    55681 tgatacacca aaccatatac aacttcattc ctgtcaggct ttcctgtctg aattaaatct
    55741 ttagcttctt gtgaaataat ctctttattt acatagtgat aggtggctgc gtccactaca
    55801 gaaggagcca tcaaaaggct tccaagaaat acagagccta caactccctt aatcttacga
    55861 attgaaaaac ggtctttttt aaacactttt atctccttta ttcattctca agactttcta
    55921 atagcatctt gcggatagtg cgcacgcgca cctccgatta attttggacg actagccagt
    55981 gccgttacat gggcatgacc aatctctctc aaaatagggc gaatcggaac ctgaacatgc
    56041 ttgacatgca tgccaattgc agtgtctccg atatccaatc cagcatgagc cttgataaat
    56101 tcaacctcaa ctggatcctg cataaactta aaggctgcca actgccccga acctcctgca
    56161 tgaagagtag gatggacact gacaatttcc agatcaaact gctctgccac ctgacgttca
    56221 acaacgagag cccgattgac atgctcacaa ccttgaactg ctaaatggat acctctacta
    56281 cctagaatac ccaagatagt ctccactatc agctcaccaa tctcttgact ggattctttc
    56341 ccaatctgac cacctagcac ctcactagaa gatagaccta aaacaaaaag gaccccctgc
    56401 ttcaaattgg tcttttctaa aacatcttcc actacctgac gtgtttctct ttgaatctgt
    56461 gtctcgttca tctctgttac ctctgttgtt actcttctat cataccgttt tttcttgttt
    56521 ttagcaagat agacaaccta gaaagcttgc ccaattacgc ataaaactcc cagaattgac
    56581 tgggagttag ctagtttcta ttctatttat atatatttca actttcgtcc ctttttgggg
    56641 tctagaatca atcttcatct ggtaattggc tccaaaatga agtttgagcc gttgatcgac
    56701 attttgaaga ccaactcccc cacgtttgag ttgactttga ctactatcac cagcatcttg
    56761 gaagccaacg ccatcatcct caatacggat gaccaatccc gaatcctgtt tctggacaga
    56821 aagtttaata tggccctgac cttccttttc cttaatgcca tggtaaagag cattttctac
    56881 aaggggttgt aggaccagct tgggtaagac taaattatca aaggcaacat tttcattaat
    56941 ttcgtattcc agcttatctc catagcgttg tttctggata aagagatact ggcggacatg
    57001 attgatttcg tcagagagac aaatcaagtc tttgccttga ttgagcgcca agcggaaata
    57061 ggttgccaag gacttggtca cctgcaccac tcgctgacta tcatgaaatt cagccatcca
    57121 gatgatggtg tccaaagtgt tatagaggaa atgtggatta atctggctcg aaagggcttg
    57181 aagttggtac tgacgggtcg tttcttcctg gctacgaata gctaccatca actgatcaat
    57241 ctgatccaac atggcattaa attggcgagt tacttctctc agttcatagg caccaacttc
    57301 cttggcacga agattttgag caccagaagc aatttccaac atggtttctc tcaaatcctt
    57361 caaaggagca atccagcgtt taagactgaa ccacactaag cagagacaaa caagaagaga
    57421 tgtgacactg gccccaagca aggtccacaa gagctgactc cgaacctggt ctaacttttc
    57481 caatgatgac acgccaagta ccgtccaatc agttcctgca atcttctctt gactgacgta
    57541 ggatttgtga ccaggagtat aaccctgacc tgtctcgatg tagggtttca tagcctccat
    57601 tttgctagac gaactataaa ctgtgtgttg aggatggtag acaaattcat ggttttcatt
    57661 gataatgaag gcaaagccct gctgccccaa ctggagttga ttgagatagg cttccagagt
    57721 ttcataagaa atatccaaac gaagcacacc aagattggct ccctttgcat caacaagttc
    57781 ttgagtgaca gaaatgaccc actgactatc tgatttacga gctggagtca aaacaggcat
    57841 agctccctga tgaatagcct tttggtacca atcctcagcc atcatatcag aggaagtttt
    57901 catctgcaca ctgtcatctg tagaaatgac ctgaccagat ttggtcacca gcacaacagt
    57961 tttcaagtcc ttatctgact tcaagatggt caaaaacaaa tctcggattc cctcgacctt
    58021 gtcttgactg ggattctcag cataggccag aacatccgtc tgctgggtca aaccagtcga
    58081 ggtggtttct agttttttga tataagactg aataaagtgg ctagtctggc tgatggtcgt
    58141 ttggctgttg ccctcaatgg tggcctcaat ggctgaagaa cttgattgat agtagaaagt
    58201 tccaaccaga gctaggagaa tgagaaagac cagaaagatg gaaataacca ttctaactaa
    58261 aagagaagaa cgcttcatcg gtcttctccc ttcttaaact gacgaggtgt cacacctgca
    58321 atctgcttaa aacgttgggt aaaatagttc atatcttcaa aaccaacctt ctctgcgatc
    58381 tcataaatct tcagatctgt agttaaaagc aagagcttgg cttgtttaac acgttctctc
    58441 accagataat cctgaaaagg caagcccaac tctttcttaa tcaaggaact cagataggtc
    58501 ggactaaaac ctaagtcact ggctaaagac tttaaactaa attggctatc agccagatga
    58561 gactggattt tctgggccat gtttccttca aacctattag tcaataaatc ttgtaactgc
    58621 tcttctttct cttccttgtc tagtttttgt ttgattttcc ccaacatttc ctcaatatcc
    58681 tgacgagaaa agggtttgag caggtagtcg tccacaccta gtttgacagc agacaaggca
    58741 taatcaaaat catcgtaacc tgttaaaaag accaaatgaa cctgaggata ggtttctcgt
    58801 accagactgg ccaactggat gccatttaga tgaggcatgt tgatatcggt taaaatgata
    58861 tctggcacct gcttttggat caattcccaa gcctgccttc cattttcagc ctgaccgatg
    58921 atttccatat cgtaggctgc tacattgacc agtttagtca aaccttgtct taccagatat
    58981 tcatcttcta cgattaagat tgtgtaggtc atgctctgct cctttaccac ttactagtat
    59041 cagtatagca aaattctcct ctaactgctt aggaaagacc tcttatactc aataaaaatc
    59101 aaaaagtaaa ctaggaagat agccacaggt ttctcaaagt atagctttga ggttgta
//
LOCUS       DAVBNO010000007        58828 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVBNO010000007 DAVBNO010000000
VERSION     DAVBNO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58828
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.007"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(181..1359)
                     /locus_tag="VQ846_000408"
     CDS             complement(181..1359)
                     /locus_tag="VQ846_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET3075053.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            1639..2901
                     /locus_tag="VQ846_000409"
     CDS             1639..2901
                     /locus_tag="VQ846_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET3075054.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWSVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDSGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            2904..3800
                     /locus_tag="VQ846_000410"
     CDS             2904..3800
                     /locus_tag="VQ846_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET3075055.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            3815..4132
                     /locus_tag="VQ846_000411"
     CDS             3815..4132
                     /locus_tag="VQ846_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET3075056.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            4149..4465
                     /locus_tag="VQ846_000412"
                     /pseudo
     CDS             4149..4465
                     /locus_tag="VQ846_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
     gene            4475..5077
                     /locus_tag="VQ846_000413"
     CDS             4475..5077
                     /locus_tag="VQ846_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET3075057.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGRKGGISSSCWGSVVVTSSERLLETSLR"
     gene            4983..5609
                     /locus_tag="VQ846_000414"
                     /pseudo
     CDS             4983..5609
                     /locus_tag="VQ846_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            5624..6394
                     /locus_tag="VQ846_000415"
     CDS             5624..6394
                     /locus_tag="VQ846_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET3075058.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(6672..6959)
                     /locus_tag="VQ846_000416"
     CDS             complement(6672..6959)
                     /locus_tag="VQ846_000416"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET3075059.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCSRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            7163..7435
                     /locus_tag="VQ846_000417"
                     /pseudo
     CDS             7163..7435
                     /locus_tag="VQ846_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7545..7748)
                     /locus_tag="VQ846_000418"
     CDS             complement(7545..7748)
                     /locus_tag="VQ846_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET3075060.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(7779..8387)
                     /locus_tag="VQ846_000419"
     CDS             complement(7779..8387)
                     /locus_tag="VQ846_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET3075061.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            complement(8426..8596)
                     /locus_tag="VQ846_000420"
     CDS             complement(8426..8596)
                     /locus_tag="VQ846_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET3075062.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            complement(8608..9174)
                     /gene="amaP"
                     /locus_tag="VQ846_000421"
     CDS             complement(8608..9174)
                     /gene="amaP"
                     /locus_tag="VQ846_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET3075063.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            complement(9258..9422)
                     /locus_tag="VQ846_000422"
     CDS             complement(9258..9422)
                     /locus_tag="VQ846_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET3075064.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIIGAAIVI
                     TVVSFFTGRK"
     gene            9865..11346
                     /locus_tag="VQ846_000423"
     CDS             9865..11346
                     /locus_tag="VQ846_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET3075065.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            11708..12703
                     /locus_tag="VQ846_000424"
     CDS             11708..12703
                     /locus_tag="VQ846_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET3075066.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFWGDKTTKKI"
     gene            12830..14140
                     /locus_tag="VQ846_000425"
     CDS             12830..14140
                     /locus_tag="VQ846_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET3075067.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            14236..15138
                     /locus_tag="VQ846_000426"
     CDS             14236..15138
                     /locus_tag="VQ846_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET3075068.1"
                     /translation="MSKETVVDTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMINLVQRRVLKEDD"
     gene            15140..15985
                     /locus_tag="VQ846_000427"
     CDS             15140..15985
                     /locus_tag="VQ846_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET3075069.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            15995..17314
                     /locus_tag="VQ846_000428"
     CDS             15995..17314
                     /locus_tag="VQ846_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET3075070.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNVQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(17524..17596)
                     /locus_tag="VQ846_000429"
     tRNA            complement(17524..17596)
                     /locus_tag="VQ846_000429"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17561..17563),aa:Lys,seq:ctt)
     gene            complement(17861..19132)
                     /locus_tag="VQ846_000430"
     CDS             complement(17861..19132)
                     /locus_tag="VQ846_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET3075071.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            19402..19641
                     /locus_tag="VQ846_000431"
     CDS             19402..19641
                     /locus_tag="VQ846_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075072.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            19806..19985
                     /locus_tag="VQ846_000432"
     CDS             19806..19985
                     /locus_tag="VQ846_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075073.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            20122..20300
                     /locus_tag="VQ846_000433"
                     /pseudo
     CDS             20122..20300
                     /locus_tag="VQ846_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            20337..20789
                     /locus_tag="VQ846_000434"
     CDS             20337..20789
                     /locus_tag="VQ846_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET3075074.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            21080..21550
                     /locus_tag="VQ846_000435"
     CDS             21080..21550
                     /locus_tag="VQ846_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET3075075.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            21569..22657
                     /locus_tag="VQ846_000436"
     CDS             21569..22657
                     /locus_tag="VQ846_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET3075076.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            22638..23066
                     /locus_tag="VQ846_000437"
     CDS             22638..23066
                     /locus_tag="VQ846_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET3075077.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            23201..24154
                     /locus_tag="VQ846_000438"
     CDS             23201..24154
                     /locus_tag="VQ846_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET3075078.1"
                     /translation="MMETWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNV
                     DRFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTAL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKM
                     SIFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNP
                     GMENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDK
                     WDMVRELAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            24156..24899
                     /locus_tag="VQ846_000439"
     CDS             24156..24899
                     /locus_tag="VQ846_000439"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET3075079.1"
                     /translation="MQQYFVKGSAISPVTIENKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLTQLDQ
                     FDSIIVAYEESGKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            24910..26706
                     /gene="pepF"
                     /locus_tag="VQ846_000440"
     CDS             24910..26706
                     /gene="pepF"
                     /locus_tag="VQ846_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET3075080.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETA
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(26766..27443)
                     /locus_tag="VQ846_000441"
     CDS             complement(26766..27443)
                     /locus_tag="VQ846_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3075081.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWGI"
     gene            27690..28460
                     /locus_tag="VQ846_000442"
     CDS             27690..28460
                     /locus_tag="VQ846_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075082.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGDGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFWGLTGTEKEDLHV"
     gene            28453..29217
                     /locus_tag="VQ846_000443"
     CDS             28453..29217
                     /locus_tag="VQ846_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3075083.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TNDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
     gene            29641..30432
                     /locus_tag="VQ846_000444"
     CDS             29641..30432
                     /locus_tag="VQ846_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET3075084.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIAEVVLADGGLDKEIDKVGLSTLERSFRALVYANLLSVDANQ
                     QSVFYQGLQSEIRNVLLNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLKEVVCHPD
                     FPKNRVHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLAQEQVASWIKTVD
                     FPIEEREDFYKFSNFRTCLLEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            30543..31211
                     /locus_tag="VQ846_000445"
     CDS             30543..31211
                     /locus_tag="VQ846_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET3075085.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(31465..31998)
                     /gene="queF"
                     /locus_tag="VQ846_000446"
     CDS             complement(31465..31998)
                     /gene="queF"
                     /locus_tag="VQ846_000446"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET3075086.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(32303..32617)
                     /gene="trxA"
                     /locus_tag="VQ846_000447"
     CDS             complement(32303..32617)
                     /gene="trxA"
                     /locus_tag="VQ846_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET3075087.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            complement(32634..32855)
                     /locus_tag="VQ846_000448"
     CDS             complement(32634..32855)
                     /locus_tag="VQ846_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET3075088.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            complement(32954..33334)
                     /locus_tag="VQ846_000449"
     CDS             complement(32954..33334)
                     /locus_tag="VQ846_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET3075089.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            complement(33477..34430)
                     /locus_tag="VQ846_000450"
     CDS             complement(33477..34430)
                     /locus_tag="VQ846_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET3075090.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREARTFIQVKVDFYQKYRQ"
     gene            complement(34498..35706)
                     /gene="sstT"
                     /locus_tag="VQ846_000451"
                     /pseudo
     CDS             complement(34498..35706)
                     /gene="sstT"
                     /locus_tag="VQ846_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            35845..36405
                     /locus_tag="VQ846_000452"
     CDS             35845..36405
                     /locus_tag="VQ846_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET3075091.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            36462..37370
                     /locus_tag="VQ846_000453"
     CDS             36462..37370
                     /locus_tag="VQ846_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET3075092.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            37322..37912
                     /locus_tag="VQ846_000454"
     CDS             37322..37912
                     /locus_tag="VQ846_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET3075093.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            37915..39021
                     /gene="yqeH"
                     /locus_tag="VQ846_000455"
     CDS             37915..39021
                     /gene="yqeH"
                     /locus_tag="VQ846_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET3075094.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKFEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            39235..39546
                     /gene="yhbY"
                     /locus_tag="VQ846_000456"
     CDS             39235..39546
                     /gene="yhbY"
                     /locus_tag="VQ846_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET3075095.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            39598..40227
                     /locus_tag="VQ846_000457"
     CDS             39598..40227
                     /locus_tag="VQ846_000457"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET3075096.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            40227..40820
                     /gene="yqeK"
                     /locus_tag="VQ846_000458"
     CDS             40227..40820
                     /gene="yqeK"
                     /locus_tag="VQ846_000458"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET3075097.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDFDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            40821..41321
                     /locus_tag="VQ846_000459"
     CDS             40821..41321
                     /locus_tag="VQ846_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET3075098.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEECFADVL
                     DYKHIF"
     gene            41334..41687
                     /gene="rsfS"
                     /locus_tag="VQ846_000460"
     CDS             41334..41687
                     /gene="rsfS"
                     /locus_tag="VQ846_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET3075099.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHIEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            41790..42536
                     /locus_tag="VQ846_000461"
     CDS             41790..42536
                     /locus_tag="VQ846_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET3075100.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYEILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            42546..43643
                     /locus_tag="VQ846_000462"
     CDS             42546..43643
                     /locus_tag="VQ846_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET3075101.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKNLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKVDQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            43990..46737
                     /locus_tag="VQ846_000463"
     CDS             43990..46737
                     /locus_tag="VQ846_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HET3075102.1"
                     /translation="MEENYNLSITQIKNSIKENSLVLFVGAGISANSNLPTWGELIQS
                     LKKELNIPEERTDSPLRIAQYYYDTFGKNQYTKKIEEIFFKKGLSKPNELHKLIEKIA
                     PKHIITTNYDSLLESQFESGLLKYNVVAEDKDIPYTSSERYLIKMHGDFSKKNIVLKE
                     DDYLDYHLNFPMISTLIQSLIMNHTLLFVGYSLSDSTFNSIFRMIQNTFKLDAKNAYF
                     YTPEEPSMIIREYYKKQGIFIISNEENLGQETSEKQNKLYCRTKDFLEVLSENRSQDV
                     NNADDLWNQLAFLDRLSFIDAKDFSRYSDLKKRALNWDDEYCWFGNDQLRFEIDGHEE
                     LRIMVSKKSLLNRFLDMEIGEPRDLKGNRFLSKAFKLYEEKQYSLAKAKFRELANIAF
                     RQKDYFNFLVCEFNFQQIQIIDRYEKTPSYAEPLYDGELSELTEQILNSVTGDEKKII
                     EFFRDSILNFNFLYRKLETINELFDEIREEHESYRRGGWSANNHLFNAEFTVKNLYNF
                     LKLNCLCVEHYKIYKSIINRYLEILLLSYDNSYVNPDSSIFDRTSSWLKNLDLDDVKL
                     ILPNIDFKVVNLYFRNYSFGKIKVTEEAKDYLLNRITYLQERLEITEDENLRELKNML
                     IFLPLVDDIDIEKVIEILNNQTLYYNWSEEFRRIIKIVLDNMDVIDKDSLKSKIIGIV
                     NKHLNEILEKNFSLYNSVYPLYSQLLEYCSTDQETAIIVLEKFNTDILRIKYKNDDIK
                     NIIEYSDLICHLFKYFEKDIKDDILDTLKVYEESENIIYHKVIDLMSYNVYDFPQIQN
                     KIYHYLIKRINDKRVEGVKTFPDPREKSVSDLYNLSRKGYFSDFEILKDIEEDIRGLY
                     PEVDWTWFHDRSDDVIHRLLEHRTPNNIKTYFSKNEEDNKLINEYILKAFDEDKLILK
                     K"
     gene            47080..48693
                     /locus_tag="VQ846_000464"
     CDS             47080..48693
                     /locus_tag="VQ846_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET3075103.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            48823..49449
                     /gene="gmk"
                     /locus_tag="VQ846_000465"
     CDS             48823..49449
                     /gene="gmk"
                     /locus_tag="VQ846_000465"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET3075104.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            49474..49788
                     /locus_tag="VQ846_000466"
     CDS             49474..49788
                     /locus_tag="VQ846_000466"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET3075105.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            49854..52250
                     /locus_tag="VQ846_000467"
     CDS             49854..52250
                     /locus_tag="VQ846_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET3075106.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKTMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARIFRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            52263..53198
                     /locus_tag="VQ846_000468"
     CDS             52263..53198
                     /locus_tag="VQ846_000468"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET3075107.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            53191..54504
                     /gene="rsmB"
                     /locus_tag="VQ846_000469"
     CDS             53191..54504
                     /gene="rsmB"
                     /locus_tag="VQ846_000469"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET3075108.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSVYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIANIKRKNKRDSIAYSLPVWLVTKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANSSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            54519..55259
                     /locus_tag="VQ846_000470"
     CDS             54519..55259
                     /locus_tag="VQ846_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET3075109.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            55256..57235
                     /gene="pknB"
                     /locus_tag="VQ846_000471"
     CDS             55256..57235
                     /gene="pknB"
                     /locus_tag="VQ846_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET3075110.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            57350..58108
                     /locus_tag="VQ846_000472"
     CDS             57350..58108
                     /locus_tag="VQ846_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET3075111.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLVN"
     gene            58110..58559
                     /locus_tag="VQ846_000473"
     CDS             58110..58559
                     /locus_tag="VQ846_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET3075112.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            58621..58828
                     /locus_tag="VQ846_000474"
                     /pseudo
     CDS             58621..58828
                     /locus_tag="VQ846_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT        17983 a        10248 c        12735 g        17862 t
ORIGIN      
        1 gatcgattcg tcctgttatt atttcatttc actatacatt atgaaaaagg ggagatagga
       61 gtataattcc aaatataaag aaagcgaaca aaacagaatt ctgattttca gaaaacaggt
      121 ttattcgctt tttatgatat actccctctt tttagcctat ttattaaatg taatagaaaa
      181 ttaagaatgt agaattgtat gatacaaaca aagtgcaata gctgagtgtg cggctgtaaa
      241 atcattgtaa ggttcaataa tgacttcagg taggcgatta tagagcagtt tgggtttgta
      301 gttcatttca ctatatagtt tttggatgat gtgttgattt gttagtaaag gactatgtag
      361 gtagattttt tgagagtcaa tcatcatact gatatttaaa atggtttgac taagatagag
      421 taaggcttga tggataagtg tgattattcc agtgtcacct aattgataag cggttaaaat
      481 aacttggata tcaatatcat cggcattttt gactaaacta ggaagtagag aatagggaga
      541 ttgatgatat agaatttttg attttttgat taaccaagat tctccagcaa aagtttgtaa
      601 gcatcccttg cgcccgcagc tacattcttc tccctcagat gaaacaactg tatgccctat
      661 ttctccaatc attagatttc ctttcccata aatgtttccg tcgtagatat aagaacagtg
      721 catacctcta gcaaaatgaa aataagcaaa attagggtca ttctgttggc gactaaatag
      781 gcgtttacct atagccatac agttaacatt gttagagaaa aacaaaggct tatcaaagtg
      841 ggattgaatc atttctaagt cgatatgttg ccataaagga ttgtttgttg ttattttata
      901 atcatctaaa tagcgaccag gtagcgcgat tccaattgct tctatttcat aatcagagca
      961 gttatttaag aattgcttta gtgtctggtt aatgagttga tttccctttt cttggattaa
     1021 ctgtttagta acaatttctt tctcttcctt taagatattt cctagattat ctcctaaagc
     1081 aaaagtgaaa tgtttttcag atagttcaca accaatgtag taaaatcgtt tagcttgaat
     1141 gtctagcagg attttttttc tccctacgct tgtatcgtgt tcgtcttcac caagttctaa
     1201 gagaatattt tctttaatca gatcatttgt gatactgcta gttgttgcag gtgttattcc
     1261 tgttttagta gcgatttcaa ttcgtgaaat gggacctaat gtgtaaactg tttccagtat
     1321 tttagctctt agttgtaatt gtttcttaga taaggtcata ttagttttct tcctagttgt
     1381 cagattctta aaatcctatg attttttgct atggataaat gtagatgtta atctttttaa
     1441 gcttttcatt acttactggt ctctttttat tatatattga taataaaaaa taatcaaatg
     1501 tttacacaaa tccttgacaa ggctttctta tagttgtaaa atataaataa caaaagataa
     1561 tcaaataaca acttgacgtc aaaaaataaa gataattgat aacttttgat aataaaatat
     1621 atttaagagg aaaaggttat ggaaaaatta ttacaagaaa agttacttcc agtagcggct
     1681 agattgggta ataataaagc gttggtttct attcgagacg gtattactct aactatacct
     1741 ttattgttga taggttcatt actaatggtt attgcaagtt ttcctattcc aggatgggaa
     1801 aaatatctgg gtgatattgg tgttgctgac tatttatgga agggtgttga tagtagtttt
     1861 ggattacttg gtcttgtagc tagttttggt atcgcttact ttatggctag acaatacaaa
     1921 gttgatggta ttcctgctgg tattgtgtca ttatcatctt ttattacagt tacgcctttc
     1981 attagaggag aggctggagc aggaatgcct accgctttca tggcatcaaa aggtttattt
     2041 gttgctatga ttttaggatt gattaatggc tatatctacc aatggtttat aaatcataat
     2101 atacagataa aaatgccgga tggtgttcca ccagcagtat ctaaaagctt tagtgccatt
     2161 attcctggtg cagtgactat tgttggttgg ttgattgttt atgcaacttt agataaatta
     2221 agtttaccta atcttcatga aattgctcaa gtagctttgg gaggtccact tggactttta
     2281 ggaaataatg ttattggtct tcttatctta attttcctta atagtagttt ttggtctgta
     2341 ggattacacg gaggaaatgt tgttaatgca gttatgaaac cgttatggtt agctaatcta
     2401 gatgcaaata aagtagctta tcaaactgga gaaactcttc caaatatttt tactagcgta
     2461 tttatggata attttgtatt cataggtggc ggtggtgcaa ccataggttt agttcttgcg
     2521 ttaggatatt tagcacataa gaagaaagct agtaaacaac taaaaacttt ggcacctata
     2581 actgttatac ctggattatt taatataaat gaaccagcaa tgtttggcgt tcctattgtt
     2641 ttgaacattt tgttactagt tccatttatc ttagctccaa tgtttaactt actagtagcg
     2701 tggggagcaa tggcatcagg tttggttccg ctaacttata cagattcagg ttggactatg
     2761 ccacctgtta taagtggttt acttgctaca ggaagtattt ctggttcatt attacaaatt
     2821 gtattgattg ttttggatgt cttactttat ttaccatttg taatagctat tgaaaaacgg
     2881 tttaagttat tggaggattg agaatgaaac gtttaattag tgcaaatcca tctgagatat
     2941 tacagatgaa tgctgaagaa ttaaaacaaa gtattttagc aagtgaaggt agagttgttc
     3001 tatctgaaaa tgtagttact cgtgagacat ttgttgggga tataactaat tctgaaattg
     3061 ctagagcttt tggagctgat atgattttat tgaattgtgt tgatgttttt gagcctaaaa
     3121 tttatgcttt ggatagttca ggtgatgatg ttattcatcg cttacaccag cttgttgctt
     3181 gtccaattgg tgtaaatttg gaaccgattg acccatctgc aaagatgcta gaggaaacac
     3241 aggaaattgt tgcaggtcgt gttgctagtg ttgaaacatt gaatcgaata gaggagttag
     3301 gttttgactt tgtctgtttg actggaaatc ctggaacagg agttagcaat cgagaaatca
     3361 ttaaggctgt tcaaactgct aaggaaaact tttctggttt gattattgca ggtaagatgc
     3421 acggggcagg agtgaatgag cctgtggcag agctttctgt cgcagagcaa ttgttggaag
     3481 caggtgcgga tgtgatactt gttccagcag ttggaaccgt tccagccttt catgaccaag
     3541 agttgcgtga agtcgttgat ctcgttcata gtaagggtgg tcttgtattg agtgctattg
     3601 gtactagcca agaaacatct gatacagata ctattaagga aattgcactt agaaataaaa
     3661 tttgtggagt tgatattcaa catataggtg acgcaggata tgggggactg gcaacagtcg
     3721 ataatattta tgcattgagc aaggcaatta gaggagtgag acatacagta tctcgcttgg
     3781 ctaggtcagt aaataggtga taaaggagta agctatggct aaagtaacaa ttatgttagc
     3841 atgtgcagca ggtatgagta caagtctgct agtgacaaag atgcaaaagg cagcagaaga
     3901 taaggggttg gatgcagaaa tttttgcagt tccagctcct gaagcagaag aaattgtagc
     3961 aacaaaagaa gtaaatgtgt tgcttttagg cccccaagtt cgctatttac taggggactt
     4021 tcaagaaaaa ctaaaagata gacagattcc tgtggcggtt attccgatga cagattacgg
     4081 aatgatgaat ggttctaaag ttttagattt agctgaaagt ttattagact aagattgagg
     4141 agaatgttat ggatgaaagt aatttagaat ctgtaatggg gctaattatg tatggtggag
     4201 atgccaaaag taatgctatg gaggctattc aggcagcaaa aaaagggatt tctctaaagc
     4261 caatcgaaga ttagctgatg cgaatgctgc cttattacag gcgcataagg ctcaaacaga
     4321 aatgttgaca agagaggcac aggggggaaa aacatcaatt agcctcttga tggtacacgc
     4381 gcaagatcat ttaatgacga gtctgacttt tgttgacttg gcgaaagaag tggtagaagt
     4441 atacgaacga tttgaaaaaa attaggagtt tggtatgaat acaatgttgg ataaaatgca
     4501 agaaaaactt tctccaattg caatgaaagt tgggaatcag aagtttttag ttgctttacg
     4561 tgattcattt gtggggacta tgcctgttat tatgacaggt tccattgctt tgttattaaa
     4621 tgcttttcta gtggatttac cacaacaatt tcacctagaa agtattacga aaacctttca
     4681 gtggcttgtt gacatcaata atttggtatt caagggaagt ataccaattg tttccttgtt
     4741 gttcatctat tgcttgggag tgaatatcgc taagatttac aaggttgata cagtttctgc
     4801 tggtttggta tcacttgctt cgtttgtcat ttcgattgga agtacagtta caaagagttt
     4861 ccctttggca aatgtaggag atgtgaaatt agatcagatc ttacaaggaa ttgataatct
     4921 agcatttgat ggtaaaaatc tgatggtaac tattgggaat gtgattccag gaaaccatat
     4981 taatgccaga ggcagaaagg gagggatatc ctcatcctgc tggggctccg tggtggttac
     5041 aagttccgaa cgcttgctgg aaacatccct acggtgagaa tagtaatcta gtgggtttag
     5101 aagaccatcc agtagttcat gttgctttag aagatgcatt ggctttctgt aattggagcg
     5161 gaatgtcttt accgactgaa gcacagtggg aatatgctgc tagaggagga agacaatctg
     5221 aatatccttg gggagatact cttttagaag gtggatatta tcatgccaat acttggcaag
     5281 gaagatttcc ttatgaaaat actgtctcag atggatttat aggaacggct cctgtctatg
     5341 aatttttacc taatgatttt ggtctatatc aaatgattgg gaatgtttgg gaatggtgtc
     5401 gcaatccgag atatacctta ttagctagtt ttaatgaaga tgactatgag ttgccaaaat
     5461 atgggataca agatgaggag tatgctatca gaggaggttc ctttttatgt cattgttctt
     5521 attgtaatcg ataccgagta gctgctagaa atggatgtat ttctacttcc actagtagcc
     5581 atctaggttt tagatgtttg aaagaatagg atggacttaa aaagtggttg agattattta
     5641 ttttctaatc attataatag ctagtggatt gggttctata tcaggcatgg gtggtggaat
     5701 tataattaag ccacttatgg atagttttgg gtaccattcg gtcagtgata ttgcttttta
     5761 ttctagtttc tctgttttta taatggcgat tatttcaaca atcaaaagat tttcacaaag
     5821 taaagagata aaatggagat tgatttttac ggtatccttt tcttctgttt taggaggatt
     5881 tctaggtcat cttatttttc aagttttgtt atctcaatta tcagtacggc tagtatccat
     5941 tgttcaaatg attttactat ttgtgatgct acttgtatca tttgttttaa cagattttaa
     6001 aaaaacttat caatttgata agataggatt ctatatgatt tgtggtcttc tattgggctt
     6061 aatttctagt tttttaggga ttggtggtgg tccgctcaat gtatctttat tgatggtgtt
     6121 tttttctatt tcgataaaag aagcaacgat gtattctctt gcaattattt tcttttccca
     6181 gctgtctcat ttggctacaa ttgttgtggt tacaggttta aatcagtatc atttagcacc
     6241 tgttccagtt atttttcttg cttctatatg cggaggggta ttggggacgg ttgtatcaaa
     6301 agtcttacct gaaaattggg taagatactg ttttaaaggg atgttattct ttgtcatggg
     6361 aatgacttta tataatttat ttcatatttt gtaaggtcag tgtgaaaata tttaaataga
     6421 cttcctgtaa aaactggatt taacgccctc catttgtcaa attaagctag acagtcttac
     6481 caactcagaa aatatctgat gaggtatttg gtatccaaag gatttaggtg ggatgtgatt
     6541 tcttgaggct gggaaaaagt ctttaaaatt aaaaaatgca taatatcagg tgttggaaaa
     6601 actttgatac tatgcgtttt attgtgggaa tatttatttc attttctcct gaaattgagt
     6661 ttttgtccag cctaaaattc ttgactgact tcgttgaaat ctgtctgctt tggcaagtca
     6721 tgacacctta aaagtctatt tgaatgttcg ttgagacacc gctgtcttga acatcctaga
     6781 ttcgcaaaaa agctctcact atcgtgatga ttggaaatat ctttccattt tgaaaactct
     6841 tttccacagt caaacgtaat agatttaaag aggttcacta gaaattgtgc cagccaccct
     6901 tggatggatt ctgccatatc ctttgcctta cgactcttgg tcttcaaact gacaatcaat
     6961 tgactatacc tgttcgctca caaataggag agaactgttt acgaaaatct aatgaatatg
     7021 ccacaagaac attataccgc aacgtgtact atttttggtt cattttacta tagaaactat
     7081 tgaagaacct gtacgaaaat atatcatgaa aaagggtaga tagaaagcaa acagccttga
     7141 aatcaatgtt gactgcgatt atgaacctga atcagcccct caaaagggac tgtatatgaa
     7201 tgaacgttac cagtgtttaa aaactaaaga atatcaggca cttttatctt ccaagggtag
     7261 acaaattttc gctaaacgta agattgatat gaaatctgtc tttgggcaga taaaggtttg
     7321 tttgggttat aagagatgtc atctgagagg gaagcgtcaa gtgagaattg acatgggatt
     7381 cgtactcatg gccaacaacc tgctgaaata taataagaga aagaggcaaa attaaaaagc
     7441 tagagttccg caattgggaa tccctagctt tttgttttct gagaatcatc ttgactcaga
     7501 ctcttttgac taatgttact aaaaagataa agtaactttt aaccttattt atcgtcgcca
     7561 ctcaacatgt ttttaacacc ttctacagca ccttctacag cgtctttggc atcttcggcg
     7621 acttctttta ctttagaaac aactttttca gctgctcctt ctttttccat tttttcatca
     7681 ccgatggctt tcccaacacc ttctttaata gaacctttag cttgatttaa tttttcttct
     7741 actgacatga taatttctcc tgttatattt attttatctt agtttacacg agtgttttcg
     7801 tgagatacta caccatttgc tgcaccttta acagcttcta caccttcgct aactttttct
     7861 tgaacagttg agaaaccaga tccaagacca gatttagctt tttcgaattg ttctgaagtg
     7921 aattctcctg ttgattcagc aacgtcagat acgcgatctt gaaggcttac tgagtctgct
     7981 tcatgctgtt ctttagtttt gatgtcgaca acgtttacgt tgatttcaac aatttccaag
     8041 tcagtcattt tagcaacttc tgaagatacg atttctctga tttctgaata taaagctgga
     8101 acattttttt ggtactcaac aataacgttt aagtcaactg caacttgtgt tttaccaact
     8161 tctacgttaa caccacttgt tacgtcatcg ctgttaacga ttttttcttt aagatttgag
     8221 aagaaaccac catcgattcc caaaagacct gaaacgtttt ctagtgaaag accaatgatt
     8281 ttttggataa ctttatcttc gtaagtaagt tcccctttga tttcgtgagc tacaacagta
     8341 gcatctttct tttctacgtt agtgtttgtg tttttttcgt ttgacatatg aaactccttt
     8401 aattaaatta gtaattttta tttttttatt tatctatata gtttttttcg atatataatc
     8461 cagctgcaac tcccagtgct cctaaaatca atacaaatat tgttttgaag aagccaaagg
     8521 agacaatcaa acaagccaga aatacgccta tgagaccagc gataattgga tatcgatatt
     8581 gtttaagcca ttccattttt tacttcctta cttcacacga ctaacagtct ttttgttttg
     8641 aggttttggt tggtaatttt ttactgcaac ttcaagtttt acttgacgct caataccaaa
     8701 aaactgcttc aatccattag ttatttcatt ttgaattatt tggcatctgt cagcgatgtt
     8761 gtctgaagga agaattttac cttctacatg aacgaaacat ttattttttc gtaaatttac
     8821 atgaacagtt gggttcttga tcaatctatg atcactcacc aaacttctaa caaaaccttc
     8881 gattgccgaa ttctttaatt ttaatgtatc gtttttagtt tcaagttgga tttccaagta
     8941 acgtttagga taaaacatca caactaaaat ggataataaa actaaagttg atagaaccac
     9001 tgtcccccaa aagacatatc tagcaagata gaattcaact acggagttct gtctccagct
     9061 aagtagatga atacctagat cactaacttg atgataatct atcaaaacag gaaggaaaat
     9121 tgtcaagatt aaaatacaga aaataatgaa acatattttc tttgcttttg acatatacaa
     9181 tcctttcgat ttaacattta aactattttt cccgtcattg atttaaaaat agtttgctaa
     9241 ctttactggc gaaaagttta ctttctacct gtaaagaatg acactacagt aatcacaatc
     9301 gctgcaccta taatagatgg gagcaaagcc attccagcga ttgatggacc ccaactacct
     9361 aaaagagatt gtcctgcata tgccccgact aaaccagcga atacatttgc gatgattccc
     9421 atagaactac ttttttagtg attttacctg ccaagagacc aataagacct ccagcaataa
     9481 ttgatcacaa cactgcctac cctccttttt tattttaata tcaaaaaatc aatttctttg
     9541 attaatacta ttatataact ttaatataga aaagtcaatt atttcgattg ttattattat
     9601 ataattttaa aaaatcaaat tctttaattg ttgctattat ataattttta aaattaaaaa
     9661 tataatcttt agacttgaat ttgattctgt attcacgccc tcaatagacg gtaaaataag
     9721 aaaattgttt atatattgtc tccgtagtgt tattatacga aataaaagat tttaggaaaa
     9781 gtcgttatat catgctatac ctattctttg tggtataatt gcaagaggtt taatccgata
     9841 atttataaag agagaaagat attcatgaga gatttattat ctaaaaaaag tcataggcaa
     9901 ttagaattat tagaattatt atttgaacat aaacgttggt ttcatcgttc tgaactagca
     9961 gagttattaa attgtacaga acgtgcagtc aaagatgatc tatcccatgt taaatctgct
    10021 tttcctgact tgatttttca ttcttctact aatggtatac gcattattaa taccgatgat
    10081 agtgatattg aaatggttta ccatcatttc tttaaacatt caactcattt ttcgatttta
    10141 gaattcatct tctttaatga aggttgtcaa gctgagagta tttgtaaaga attttatatc
    10201 agttcatctt cgctctatcg tattattagc caaatcaata aagtgattaa aaggcaattt
    10261 caatttgaag tcagtctgac ccctgttcaa atcattggaa atgagagaga cattcgttac
    10321 ttttttgcac aatatttttc agaaaaatat tatttcctag aatggccatt tgaaaatttt
    10381 tcatcagagc cactatctca attgttagaa ttggtttata aggaaacaag ctttccaatg
    10441 aatttgtcaa cgcatagaat gctaaagttg ctcctagtta cgaacctata tagaataaag
    10501 tttggtcatt ttatggaagt agataaagat tcttttaacg accaaagttt ggatttttta
    10561 atgcaggcag aaggaataga aggtgttgct cagagttttg aatcagaata caatatctct
    10621 ttagatgaag aagttgtttg ccaattattt gtgtcttact ttcaaaaaat gtttttcata
    10681 gatgaaagtc tctttatgaa atgcgtaaaa aaggatagtt atgttgaaaa atcttaccat
    10741 ctattgagtg attttattga tcagatttca gtcaagtatc agattgaaat ggagaataag
    10801 gataatctga tttggcatct gcataatacc gcacatctgt atcgtcaaga gttgtttact
    10861 gagtttattt tatttgatca aaaagggaat acaatcagga actttcaaaa tatttttcca
    10921 aaatttgttt cagatataaa aaaagagctt tctcactatt tagagactct tgaggtttgt
    10981 tctagttcaa tgatggtaaa tcatctatct tataccttca tcactcatac caaacatttg
    11041 gtgattaatt tgctacaaaa tcagcctaaa ctaaaggttt tagttatgag taattttgat
    11101 cagtatcatg caaaattcgt tgcagagaca ctttcttatt actgtagcaa taattttgaa
    11161 ctggaagttt ggactgaatt agaattatca aaggaatctt tagaagattc accttatgat
    11221 atcattattt ctaattttat tattcctcct attgaaaata agagactcat ctattcaaat
    11281 aatataaaca cggtctcact catatacttg ttaaatgcca tgatgtttat tcgattagat
    11341 gagtaaccaa aaataacaaa aaaacgctat ttcgcgtttg aactgtacct caaaagttag
    11401 acagaaaaaa tctaattttt ggggtgtttt tgttaagaaa agaccttcaa tcatttaagc
    11461 aaaatgtaca tctagattta tagccctcta aaactcttgc tttttttgta gaagatacga
    11521 aaaaagccat aaaaccaagg cttttcctgt tttatgtaga tgtttacaca tgaaaattga
    11581 agtattttca aaagctaatt ggagtgattt tattcatgat gggataaatg atctggccaa
    11641 aaatgattga gaaatagttg cttaaaaagc atttgcatga tactatatat tgaaaggagg
    11701 tgataatatg gtagaacaaa gaaaatcaat taccatgaaa gatgttgctt tagaagcagg
    11761 agttagtgtt ggaactgttt cacgtgtaat taataaagaa aaaggcatta aagaagtaac
    11821 tttaaggaaa gttcaaaggg ctataaaaac attggactat atccctgata attatgctcg
    11881 aggtatgaaa aagaatagaa cagaaactat cgctttgatt attccgagca tatggcatcc
    11941 atttttttcg gagtttgcct atcatgttga gaaagagtta ttaaaatata attataaatt
    12001 atttatttgt aacgctgata gcgattcaca aaatgaaatc gaatatatcg aaatgttgaa
    12061 acaaaataaa gtggatggaa ttattggtat tacatattct gatattgaca aatatatttt
    12121 atctgattta ccgtttgtaa gtatagatag acatttttcg gaaaatgtat gctatgttac
    12181 ttcagcaaat tacaaaggag gacaaactgc agcagaggaa ctggttagaa gggataataa
    12241 gcaccttgct tttattggtt caattaatat gtatgagaat gagactatac tcagaagtaa
    12301 aggattcaga gaaaaagcta aggaattagg agtagatgtc tataattttg aaatgaaaga
    12361 acctttaata aatcctgaag atcagataag aatttttttg caatctcata aagaaattga
    12421 tggtatattt acaataaatg atatgatggc tctgagagtt attagtgtta tgaaagaact
    12481 ggggaaaata gcacctgatg attaccagat aattgggttt gatggtttaa aaacttctgt
    12541 cgatcaatct tatctagtgt ctacaatagt tcaagatatt ggtatgatgg caaaagaatc
    12601 tgttgcaatg atactgaaaa tgattaagcg agaagagatt ctagaaaaac gaatcgtaat
    12661 tcccacatat ttttggggag ataaaacaac aaaaaaaatt taaaaagcgc ttgacaatta
    12721 atgaaagcgc tgttataata taatcaacaa atgaaacgtt tcaaaaatag gctagaagct
    12781 tgtttaaaaa ataaataaaa aatgaaacgt ttcaaaaagg aggaaaatga tgcgtaaagg
    12841 tacaatgtta tctgttgttg cggggctatc gttagctata cttgcaggtt gtagtggagg
    12901 aactaactca aaacaagcga gttcttcaaa tgatattaaa gtctgggtac aattctctga
    12961 cgaaacaaat gaaggaaaag cttggcaaaa agttgttgat aactttaatc aatcaggaaa
    13021 aagtaagtac aaggttgtaa ctgagtatat tcctcgtagt ggtagtggtg gaggatatga
    13081 agacaaggtt aatgctgcta ttacgactaa tagtcttcca gatgttatta cccttgatgg
    13141 accaaatacg gcagcttatg caaaatctaa agtaattact ccgctagatg attatctaaa
    13201 agataataat atggatgatg ttttggacag tattaaacaa cagggaactt ataatgggaa
    13261 attctatgca tttggatttt ctgaatcgaa tgttggtgtt tattacaata aaaaaatgtt
    13321 caaagaggcc ggaattgcgg aatcagagct tccaacgcta gaaaagccat ggacatggga
    13381 cgaatttaat acgattgcta aaaaattgaa agatcattat aacaaaccag caattgattt
    13441 tcgtattaat tcaaacgatg aaatgttgcc atatgcttat atgccgttga tttggtcaaa
    13501 taatggttct gtcgtaaacg aagatggaac aaaggcagaa ggatacttca actccaaaga
    13561 gagtgtagaa gcagttcaat tcattcaaaa tcttgtaaaa gaaggctata caactgttag
    13621 tccagtcgaa aaaggttttg aaacaggaga gtatccaatg cttttgagtg gttcttggac
    13681 gattgctgac atgaatacaa actacaaaga tattgatttt ggtatccttc cttacccagt
    13741 atctagtaag acgaaaaaat tagtatcccc ttcaggtagt tggcaattag ctgtgacgac
    13801 aaaatctgat aaaaaagagg ccgcatctga atttgttaaa tttgctacaa atacagaatc
    13861 aagtgaaatt attagcttgg ggaattcggt ccttccaatt cgtaaatcta caattgaaaa
    13921 tatcaaagat aaagtatccg aaccaatgcg tttcttgatg gaacaaaatt caaaaacagc
    13981 tcatgctcgc ccagttgttg tagcatatcc tcaggtttct cgagcttttc aacaagcaat
    14041 gcaagatatt agctattatg aagaaaatcc aaatgtgcaa aaagtcttgg atactcgtac
    14101 aaaagaaatg caaactgcta ttgatcagtc actcaaataa tctgtaaaaa agaaggtgga
    14161 aggaaaatgt ttggaactac cttccccttt cttgttaata gcctagtttg atatatatga
    14221 ttaaaggaga tggatatgag taaagaaact gttgtggata caaaaagacg tgaaaaaata
    14281 aaggataaca tattaggtta tagtttttta gctccagctt taattttatt aggtatcttt
    14341 ttagtaattc cagttggaat ggtcatttat tacgctttta cagattatta tttgttgaca
    14401 ccagatgaaa gaaaatttgt tggacttgaa aattttattc gattaacaaa agatccgatt
    14461 ttcttaaaga gcttcttgaa tactttgaaa tttgttgttt ggattattcc agttcaattg
    14521 ggagcggcct taggaatggc tttgattgtc aataaaaagc gaaaaggaaa tatgtttttt
    14581 aaagtggctt tttttgctcc agttgtcatg tccttggtag ttatttctat tctttggctt
    14641 tatcttttaa atccaaatag tggcttattg aatgctattt taaataaggt aggtattgct
    14701 tctcaacctt tcttgactag tcctaaacaa gctatgtatg caattgtttt tgtttctgct
    14761 tggcagggtg caggttatca gatgctttta tttttaggag gaatgcagaa tattccacaa
    14821 gatgtctatg aagctgctga attagatgga ttctcaaaat gggctcagtt tcgttatatc
    14881 acgatgcccc tactaaaacc aacagccctg tttgtattgt taacaacttt aatttctgct
    14941 tttaagctga ttgtacaacc aatggttatg acgcaaggtg ggccattaaa ctcaacaatt
    15001 actatggtgt actatattta tcaacaaggt tttactgatc gtcttgtagg atattctagc
    15061 tcaatcgctt tagtatttac aacgttaatt ggtatgatta acttagtaca acgtcgcgtt
    15121 ctgaaggagg atgactaaga tgaaacgatt taaaccaaca acaatcttag aatatattct
    15181 gttaacttta ttggcaggac tcttcctttt tcctatggtt tggatggtag tttcagctat
    15241 gaagccagaa gcggatgttt ataaaaattt gactagttgg caagccttct taccaagttt
    15301 aaatcccgct gattggttta aaccttatca ggaagtattt gagcgcttta gtatttttac
    15361 atatctaggg aacagcattt tttatgctgg aacgtttgct attggttcta ttattgtcaa
    15421 tgcactggcc ggtttcgctt ttgcaaaagt caatttttca ggtaagaaaa ttttatttgg
    15481 tttcttactt gctttactta ttattccagt ggaaactgtg ttaattccac aatttaccat
    15541 tatcaattcc ttaggtttgg tcaataatcg tcttgcagtt attattccag gattggcgag
    15601 tgtatttaat atttacttat ttaggaattt ctttattgca attccagaag agattcttga
    15661 gtctgcaaag atggacggag caagtatcgt tagaattttc tttagaatta tgcttccaat
    15721 gtctaaacct gcagttgcta cagttggtgt tttatcattt atttcaagct ggaatgatta
    15781 tatctggcca ttgatggttt tgacagattc ttctaaattt tcaatgcagg ttgcaattac
    15841 aactattaat acaactcaac cagtttatat caatcaggta atggctgtat taactatttc
    15901 aacgattccg ttgattttgg tctatgtagt cgctcagaaa tatattgttc aaggtctggg
    15961 tggttccgga actggaataa aataggatat acttatgata aataaaacat ttacagtaga
    16021 aaaagccaat cgttttatag cagaaaataa acatctcgtt aatactcaat ataagcctga
    16081 agaacatttt tcagctgaga ttggttggat caatgatcca aatggatttg tctattttcg
    16141 tggagaatac catctctttt atcaattcta tccatatgat agtgtttggg ggcctatgca
    16201 ctggggacat gctaaaagta aggacttggt gacttgggag cacttgccag tggcacttgc
    16261 tcctgatcaa gattatgacc gaaatggttg tttctcaggc tctgccattg tcaaggatga
    16321 tcgcctctgg ctcatgtaca ctgggcatat cgaagaaaaa accggtgtcc gccaagtgca
    16381 aaatatggca ttttcagatg acgggattca ctttgaaaag atttcccaaa atccagttgc
    16441 aactggatca gacttgccag atgagttgat tgctgctgat ttccgtgatc caaaactctt
    16501 tgaaaaagat ggacgctatt actccgtagt agctgccaaa cacaagaata atgtgggctg
    16561 tatcgttcta ctagggtccg ataacctagt agaatggcag ttcgaatcca tctttttaaa
    16621 aggggtagaa caccaaggct ttatgtggga atgcccagat tacttcgagt tagatgggaa
    16681 agattgcctt attatgtcac ccatgcgtta tcagcgtgag ggagactcat atcataacat
    16741 caactcatcg cttttgttca cgggtaaggt agattgggga gaaaaatgtt ttatcccaga
    16801 atcagttcaa gaaattgatc atggccaaga cttctatgcg cctcaaacat tgttggacga
    16861 tcaaaatcgt cgtatcctga ttgcttggat gcagacatgg gggcgtaccc ttccaaccca
    16921 tgaccaagaa cacaagtggg catgtgccat gactttacct agaattctaa gattggaaga
    16981 tggcaaacta agacaattcc ctgttaaaaa aggccaatat caaatccaaa tagataaaga
    17041 ttgtcattac cacttaggaa atgatacaga ttatcttgaa tttggttatg acagtaatgt
    17101 gcagcaagtt tacattgatc gtagccatct tattcaaaaa attctaggtg aagaagaaca
    17161 ggacactagt cgacggtatg tagatattga agctaaagaa ttggaagttg ttctagataa
    17221 aaattccatc gagatttttg tcaatcaagg tgaagcaagc ttgactgcaa cttattactt
    17281 aacggtgcca gctgagctat cacgaattga ttaaaaatta agttatttct cctaaagaaa
    17341 aagttctctt tctaaaatag tggaaagagg actttttgtg ttttgggtat ataagcttag
    17401 tttatggtat ttgtaaaatt ggtgttggat tatgatttaa actagttttc taaagaattt
    17461 gaaaaaaatt ttatttaagc aaaaaaagcc ctatattata agacttttcc tgttgtattt
    17521 atatgccccc tacagggatc gaacctgtga cccacggatt aagagtccgc tgctctgcca
    17581 gctgagctaa ggaggcaaag aaaaaagctg tattggtgcc gaaacttcac ggtttgtatt
    17641 gaacccgcgc aattaagcag gtgggcaact cgctctaact gaagctgttt ccgtgtgaga
    17701 cggcctacat gctgttagaa gacttttgtt tccctaataa tacaaaaaat agtcggtcaa
    17761 cacttaagtg tgaagtcgta caccacagcg tttctatatt tatatgatac cactttttta
    17821 aaaaaaatca agaggaaagt gcaatttttt gaaaaggatt tcagattttt cgcaaatggt
    17881 cgatagcttc ctttctttga gccaaagccc gttcatattt accagtatct tctgcttgga
    17941 aatagtgatg attacgaatt ttttctggca gatagtcttg ctttacccaa tttccaggat
    18001 agttgtgtgg atagagatag tcttgggcat tccccagttc cttgcttcca ctgtagtgcc
    18061 catcacgtag gtgtcgcgga ataggcaagt gccctgatgt tttgaggtca gcaagtgcct
    18121 tatccatagc tacataggct gagttggatt ttggagaaag ggccaaatca atcacgacat
    18181 tggcaatgag aatgcgggct tcggggaaac caatcctttg tgcagcatcc agagcagtca
    18241 cggtatgaat ctgggcctca ggattggcta agccgatatc ttcataggcg ataacagtca
    18301 agcgacgagc gagactaggc agatccccag cctcaatcaa gcgggcagta tagtggagac
    18361 tggcatccac atctgagcca cgaatagact tttgcagggc agatagaaca tcatagtgac
    18421 cgtctccatc cttgtccata gtgatgtagc ttctctgaag actattttcc atgatgtcta
    18481 aggtgatgtg gcgaatgccc tcgtcattct caggggtaga tagaacagcc aagtccagtg
    18541 agttaaaggc agagcgaagg tctccgtttg tagaggttgc gatgaaatcc agcgcatcct
    18601 catctagttc tattggaaaa tcaaaaccac gttcagggtt acttagagct atctgcaggg
    18661 cctctttgac gtcttggtta gacagaggtt ccaactcgaa aatttgaaca cggctacgaa
    18721 tggcaggagt gacagagaag aaaggatttt cagtcgttgc tccaatcatg atgaccagtc
    18781 cactttccaa gagaggcaag aggaagtctt gcttggtctt atctagtcga tgaatttcgt
    18841 ctagcaatag gacgagacca ccagaaaatt tagcttcttc cgagatttct tgcagtcgct
    18901 ttttactatc aactgtcgca ttgaaagttc gaaaggcata cttggtcgtt ccagcgatgg
    18961 cagaggcaat actggttttg ccgattcccg gagggccata tagaatcatg gaggacaggc
    19021 ggttggcttc caccatgcgg cggatgattt ttccaggtcc gaccagatgc tcctgaccga
    19081 tgacctggtc gatggtttta gggcgcatgc gaagcgcgag attgtctggc atagcagtcc
    19141 tttctaacgt ggatttgagt agaatgactc atctactata tcttactatg tccattctag
    19201 cctattagtt gtgatatttc aatggttttg taataatcag ggagtgagat agaatctttt
    19261 ttactattta atttaatgag gagaggtatc agcatgagag atttgatttt ccttaaagct
    19321 caaaatagga tagttgagcc taaagaagtg gaagtgctga cttgagaaat aatattgctg
    19381 aatgtcattg tatgaactat aatgaaaaga gaggtgataa gcaatggaaa tgatggacca
    19441 agtcaggaaa ttttgatttt tacaaaacaa attcgtcatt ggattcttag tgatcaagtt
    19501 atttcaggga aaagaaaact tttcttccgg gaagatactc caaaagagat tttagatttg
    19561 tacgaaaaca ttaaatctaa acttgatttt gcttaccaag aagttcattc taataatggg
    19621 ttgaaaaaat atgaaaaata gaataattga tgtttttgaa gtagtaaatc gcctccttgt
    19681 aataactgtt gaaaatcctg atttcgaaga cttgagagtt aatcatactc aatgaaaatc
    19741 aaagagcaaa ctaggaaact agccgcaggc tgtacttgag tacggtaagg cgacgttgac
    19801 gtggtttgaa tttgattttc gaagagtatc agtttgtaaa aattggagat aaaaaatata
    19861 gagtacgcag tgttcctatg attcattcaa ctccgcctca atctgtctta gatcgagata
    19921 cttttacaat tgactataca gatgatgaat tgctcgataa agaagcagtt tttactactc
    19981 attagaaaaa taggtatgaa taaattggct tcccttagaa ccacatctag gggcttttat
    20041 gtataataga ataataagta tcactccttt acaatacgtg taacacgtgt tataatggtg
    20101 ttgtcaggag gtaaaagcgc tatgcctatg acacaaaaag agatggttaa actccttacg
    20161 gcccatggtt gataaagact agaggcggta aaggttccca tattaaaatc gagaagcaag
    20221 gggaaagacc tatcacaatc cctcatggtg agctgaataa gtacactgaa agagggataa
    20281 gaaagcaagc tgggttgtaa aattcagtcc ctgctttttg atatggaggt agtgaaatgt
    20341 tagttacgta tccagcttta ttttattatg acgacacaga cggaacagaa gcgacttatt
    20401 ttgtccattt cccagatttt gaatactcag ctacacaagg agaggggatt tctgaggctt
    20461 tggctatggg gtcggagtgg ctagggataa ctgttgcaga tttgatagag agtgatggag
    20521 agttacctca gccatcagat atcaatagct tgtctttaat tgataatgat ccttttaaag
    20581 atgatgaaga tttcgtgtca acctatgacc ttgataaatc ttttatttct atggtatctg
    20641 ttgatgtatc agaataccta ggaagtcagg aacccattaa aaagactttg accataccaa
    20701 aatgggcaga taagttggga cgagaaatgg gacttaactt ttctcagact ttgacagacg
    20761 ctattgcaga taagaaagtt caagcctaag attaagcgtg gtatcgtggt aatagtcaat
    20821 aaaaaagcac gtccacttgt gctagttact cgcctattga ttttatagat ttattagccc
    20881 tttcgagggc tttttatatt gatttttatg agaaatgaag aaatcagact tttaagaaaa
    20941 tcactgatac caagggttta aatgaggtaa tatggtataa ttaggacata aaataatttt
    21001 gtggtaagat ggtagtatct attttagcat atttccgagc aatcggggcg attaaagagt
    21061 cgcatagaaa gaggacaaaa tggcaacata cggattttta gatgttttag aggaagagtt
    21121 ggacaagaac tttccctttg actttgagat tagctgggac aaacgcaacc atgcggtaga
    21181 agtgagtttt ctattggaag cgcaaaatgc tgcaggtgtg gagatggtgg atgaagacgg
    21241 agaggtttca tcagatgaca ttctttttga ggaagcggtc cttttctaca atcctgccaa
    21301 gtcaaccgtc aatgcagaag actatttaac ggttatccct tatctaccta aaaaaggatt
    21361 ttctcgcgag tttttagctt attttgccct tttcctcaaa gatactgcgg aggttgggct
    21421 agataccctc atggactttt tggaagatcc agaagcagag gaatttgtta tggaatggaa
    21481 tcaagaagtc tttgaagaag gaaaagttgg cttggaagag ggagaatttt atccttatcc
    21541 aagatactag gagttggttg gaggtgttat gaagaaaatt gggacttatt tggtttatgt
    21601 gctagctgtt gtctttatta tgctagcttt tgcttgtgga acaatcgcat ttgcagagtt
    21661 ggggtattcc gcagttttag cctttacttt tggttatgcc ttcgctcttc taagcatgta
    21721 tttaatcttt attcttcatg agctgggtca tgctttttgt ggctacttga caggctatcg
    21781 gctggtggct tttggattag gacattttat tttgaccaaa aagtcaggca ggtttcatct
    21841 tagtagaaca gccattctga aaaatgttgg tgctcaatac attggtttaa aagaggatga
    21901 aagcgatcaa agaatcatcc tgatgctttc aggaggcttg atggttcatc tcggcttgat
    21961 attattggcg atagtgtttg gctttttgac aagaagctgg tattttgcag ggacttggat
    22021 ttttcttaat ttatctttct tcctaaataa cattttgcca gtcgacatca ccgatggagc
    22081 aaaaatttgg gaattactac aacaccctga aaataccaaa tacgcctacc tggtattgag
    22141 acattctgct caaactttgc tggctcctca agaatatgat ttgaaagact ttgttcaagc
    22201 agttcctgag gatgcacgag ggagttttgc ggatggtgta ttaggtatgc aaggtgaagt
    22261 ttccattttg gaagggaagg aagaagtagc taaacagcag tttcaagcat tacttgagag
    22321 gacaactact ccgatgatgc agacagttgc ccaactatcc ctgcttcata tcgccttgtt
    22381 ggaaggagat tttgagcaag cggagcaata tgctagtatt cgacaggtca aatcgtttct
    22441 gtctctcaaa ctgtccaatc ttcaagtgat acaagcctgg tatcaattaa aggtgaagaa
    22501 agatgtagat cgaactcgta aggctctcaa gattgctaga cagaaaatgg atagtagtcg
    22561 aatgttgagg gacgagaaac gctattatga aaacttgtta gcagagctgg aaaaggaaat
    22621 gttagaagga actgaagatg gaactagaaa tttctgattt ccaaggttgc aagattgccc
    22681 tcttttgtgg ggataagcta ctgacgattt tgcgtgatga caaggacgat atcccttgtc
    22741 ccaacatgtg ggagttgcca ggtggtggcc gtgaagggga cgaaagccct tttgaatgcg
    22801 ctcggcgtga agtttatgag gaactgggaa ttcatttaga tgaagactgt ctgctttgga
    22861 gcaagattta tcctagcgta atttttaaag gcaagaaatc ggtctttatg gttggtcagc
    22921 taagacaaga acaatttgac aatatcatct tcggagatga aggacagggc tatcagctga
    22981 tgaatgttga ggaatttctt agttccagtc aagttgtgcc tcagttgcaa gagagattaa
    23041 aagattattt aaaagtaagt gattagaaag gatggttcag ttaaatttct aaactgaacc
    23101 cgccctaaac actgtgccaa aaagataaac ttctcttaga cacaagcgtc ttcagagaat
    23161 ttcctatttt ggttttgtgt tttacgggct tggtatctta atgatggaaa catggcaaga
    23221 gttaaaagtt acagtgaagc gtgagggaga ggagttggtt tccaatctct tgattgagct
    23281 gggagcgcaa ggtgttgcga ttgaagacag tatggactat gtgggaaatg ttgaccgctt
    23341 cggcgagatt ttcccagagg tcgagcagca agaagaaatc gtagtgacag cctactaccc
    23401 tgacacggtg gatgtgacag tggttgaggc ggacttgcag gctcgcatat cagaattgac
    23461 ggattttatg gatttgggag agttaaaaat aggtacgact gccttggctg aggaagactg
    23521 ggcagataac tggaagaaat actatgagcc agctcgtatc actcatgatt tgaccatcgt
    23581 tccctcttgg acagactatg aggcgactgc gggagaaaaa attatcaagc tggatcctgg
    23641 catggctttt ggtacgggaa cccacccaac taccaagatg agcatttttg ccttggaaca
    23701 ggttcttcgt ggtggcgaaa cggtgctaga tgtgggaact ggttcagggg ttctctctat
    23761 tgccagctcg cttctggggg ctaaagaaat tttcgcctat gacctggatg atgtggcagt
    23821 tcgtgtggct caggaaaata ttgagctcaa ccctggaatg gaaaatatcc atgtagcggc
    23881 aggcgatttg cttaaaggcg tggagattga agcagatgtc atcgtggcaa acatcttggc
    23941 ggatatcctc attcatctga cagaggatgc ttatcgcttg gttaaggatg aaggttacct
    24001 gatcatgagt ggcattatca aggataagtg ggacatggtg cgcgagttgg ctgaatcagc
    24061 tggatttttc ctcgaaactc acatggttca aggggaatgg aatgcctgtg tctttaagaa
    24121 aaccaaggat atctctggtg tgattggagg ctagcatgca gcagtatttt gtaaaaggca
    24181 gtgctatctc tcctgtcact atcgagaaca aggaaaccag taagcatatg tttcaggtta
    24241 tgcgcttgaa agaagaggat gaggttacct tagtctttga tgatggcatc aagcgcttgg
    24301 cgcgcgtgct ggatatggaa aatcgtcagt ttgagttggt ccaagaatta aatgacaatg
    24361 tggaactacc agttcaagtg accatcgcat ctggatttcc caagggagac aagctggagt
    24421 tcatcgctca aaaagtaacc gaactgggtg ctagccaaat ctgggccttt cctgcagact
    24481 ggtcagttgc caagtgggat ggcaagaaat tgggtaaaaa agttgaaaaa ctagaaaaaa
    24541 ttgcccttgg agcagccgag caaagcaagc gtaatattgt accaagtatt aaacttttcg
    24601 agaaaaaagc agattttcta actcagctgg accagtttga ctctatcata gtagcctatg
    24661 aagaatcagg taaagaagga gaagccgctg cgcttctgca agcagtcact ggtcttgaaa
    24721 caggagccaa actgctcttt atctttggtc cagaaggcgg tctctcacct gcagaaatcg
    24781 aaagttttga agctaaggga gccgttttgg taggcctagg tcctcgtatt ttgcgagcag
    24841 aaacagcacc gctttacgcc ttatcagccc ttagtgtttt agtagaatta gagaaataag
    24901 aggaagaaaa tggaacaaaa acaccgttca gaatttccag agaaggaact ctgggactta
    24961 acagccctat accaagaccg tgaggatttc ttgcgtgcaa tcgagaaagc tcgcgaagac
    25021 atcaaccagt ttagccgtga ttacaagggc aatcttcaca cttttgagga tttcgagaag
    25081 gcctttgcgg aattggaaca gatctacatt cagatgagcc atattggcaa ctatgctttt
    25141 atgcctcaga cgacggacta tagcaatgac gaatttgcca atattgccca agctgggatg
    25201 gaatttgaaa cagctgccag cgtagccttg accttctttg acgatgcctt ggtggcagca
    25261 gacgaggaag tcttggaccg tttgggtaaa ttgccacatt taacagctgc cattcgtcag
    25321 gctaaaatca aaaaagccca ctacttaggg gcagatgtgg agaaggcctt gacaaatctc
    25381 ggtgaagttt tctatagtcc gcaggacatt tatactaaga tgcgagctgg ggattttgaa
    25441 atggctgact ttgaagccca tggcaagacc tataaaaaca gctttgtcac ctatgagaat
    25501 ttctaccaaa atcacgagga tgctgaggtt cgtgagaaat ccttccgttc cttctcagag
    25561 ggacttcgta agcaccaaaa tacggctgca gcagcctatc tggctcaggt caagtctgaa
    25621 aaactcttgg ctgatatgaa gggatatgac tctgtctttg actatcttct agctgaacaa
    25681 gaagtggacc gtgtcatgtt tgatcgccag attgacctca tcatgaagga ctttgcacca
    25741 gtcgctcaga gatacctcaa gcatgttgcc aaggtaaatg gtcttgaaaa gatgaccttt
    25801 gcagactgga aattggactt ggacagcgcc ctcaatcctg aagtgactat tgacgatgcc
    25861 tatgatttgg tcatgaagtc ggtagaacct ttggggcaag aatattgtca ggaagttgct
    25921 cgttaccaag aagagcgctg ggtggacttt gctgctaaca gtggcaagga ttccggtggt
    25981 tatgcggcgg acccatatcg cgtacaccct tatgtactca tgagctggac aggccgtttg
    26041 agcgatgtct ataccttgat tcatgaaatc gggcattctg gtcaattcat cttttcagac
    26101 aatcaccaaa gctatttcaa cgcccacatg tcgacctatt atgttgaagc accgtcgacc
    26161 ttcaatgaat tgctactcag tgattactta gagaaccagt ctaatgaccc acgtcaaaaa
    26221 cgcttcgctc tggctcatcg cttgacagac acctacttcc ataatttcat cacccacctc
    26281 ttggaagccg ccttccagcg taaggtgtat acattgattg aagaaggaga aaccttcgga
    26341 gcaagcaaac tcaacagcat tatgaaggaa gttttgacag atttttgggg cgatgccatt
    26401 gaaatcgacg acgatgcagc tttgacttgg atgcgccaag ctcactacta tatgggcttg
    26461 tatagttaca cttactctgc tggtcttgtc atctcgacag ctggttacct tcatcttaaa
    26521 aactcagaaa ctggagctga agactggctc aatctcctca aatcaggtgg tagcaagaca
    26581 ccacttgagt cagccatgat tatcggtgct gatatttcaa cagacaaacc actccgtgat
    26641 accatccaat tcttgtctga cacagttgac cagattatcg cctacagtgc tgagttgggg
    26701 gagtaagggg atagagaggt tgttatagaa tgggtatgga cagttatttg cttaaaagtc
    26761 catgtttaaa tcccccattc cttttcgagg aattctttgc ccttctctgt aatggtaagt
    26821 gaacgtggcg tttgtgcagg gatgaccaat tgtttttcac tgaaaaattc taaaagagca
    26881 ttgccgagag ctccgccgac atgattttta cgttcgctcc aatccaaaca tttagggcaa
    26941 aatttccgtt ttttcttttt caattcatcc gtatcaatgt gtaattttct ttggaaataa
    27001 gacttcccag cttcagtgag ttggaagtct ttttctgatt cgctgaggag attttcttgc
    27061 aagagataat tggtaatgga aatgccaagc tcaccagcaa tatggtcata gcaggtgcga
    27121 gcgaccttta attccttttt gtgaatgtgt tgtttataac ttttgacatc acgctgagga
    27181 gagtagaagc tgagaaattc cagcatttct gcaatggcag cactttttaa agaataataa
    27241 tgaaaacgtc cataggtttg tagagtggtt acctgcgctt cggtaagtaa tttgaggtga
    27301 tagctgacag tatgttgctt gattttggta aagctggcaa tttcgttaac cgtgtgagct
    27361 tttccatcca ttagaatatc taaaatgtgc atccgagaag ggtctgataa gagttttgtg
    27421 atgatgaaaa tatctggttc cataaaaatt ttctccaaat ttcatatatc gatgattttc
    27481 tatcttttat tatactataa ttaaagcaca agaaaaatag attttaaaaa tgttttacta
    27541 gagaatattt taaattttat tgtctgagtt taaatggagg taaaattaca aaagtaattg
    27601 cagcagtatc cacagttgaa ggacgttaat cttaagatta cattctcaac attttttcaa
    27661 aagtgagatg aattaacaaa gtatccaaca tgctctattt gtgggggaaa gaaagagtga
    27721 agaagttaag tgttaaaaat ctaaaaaagc aatatggttc ttatgaagct gtcaagggaa
    27781 ttagctttga attgggagac ggagagtgtt tgggtatctt gggaatcaac ggtgctggca
    27841 aaacatccac tttaaagatg atttactcac tgacctcaat ttcctcagga gagattgata
    27901 tcttggggca aaatattgaa accaatcgtc gtaagagtaa atcttgtctg ggtattgtag
    27961 ggcaggagga tatgctggac atgaccttga atgtttatga taatcttttt gctcacggtc
    28021 tatgttatgg tatctcatct gtcgaactta ggaagagaat cgattctctc ttggattttg
    28081 ttcaactgag caaacatgcg caaaagatgg tgaatcagct gtcgggtggg atgcgacgac
    28141 gtcttgtctt ggcaagagct ctcatcaatc ggcctgatat tatcatttta gatgaaccga
    28201 cagttggttt agacattcaa tcccgcaata ttatttggga taaacttttg gaattgaagg
    28261 aattaggtgt ttctatcatt attacttccc actatatgaa tgagattgaa tatttgacag
    28321 atagggtgct gatgctccac caagggacta tcaaagaaga aggaactgta gaagacttgc
    28381 tcatcaagta tgatgctgac aaccttgaga cgcttttttg ggggttgact gggacagaga
    28441 aggaggactt gcatgtttaa gttatcattt cactccatcg gacacgttgt ggtacgcaac
    28501 tacatgagtt ttagaaattt atttaaaatt tcaattgtcc ccaaccttat cgaccccctc
    28561 ttttatcttt tagcaatggg atttggagtt ggtgcttatt tgacgcatgt taacggtatg
    28621 ctctaccgtg attttgtcat tacaggttta attgctgcga ctgccatgtc tgcggccacc
    28681 gcagaaacaa cggtcaatgc ctttatccag tataagattg aaaagactta tgatgctatg
    28741 ttgatgacgc caatcaatac gaatgatatt gtggtaggtc aggctatctg ggcaggtatt
    28801 cgtgcagtga ttttcggtgg gattttttgg ctgatttcca tgcttatcac agcgcatttt
    28861 catgtcttga tgttattgat tcctctgatt ttatttatag tgggttattt gtttggtgtt
    28921 ttgggactaa tctttaccta cttggcgccg tctagggaat ttctgaatta ctacaatgtt
    28981 ttggtaatcc gtcctcttta tatgttttca gataccttct ttcccattac ttccttgccc
    29041 ccaatacttg gtgatttgac ttggttttct cccttgtatc acgctacccg catgattcgt
    29101 attatatggg gaggtgaggt gacaggtttg tatcttcatc ttatctggtt gatagccttg
    29161 gcgctcttga taacggtgct tcctatgtat atgttacatc gtagatatta tagatagtct
    29221 aattatttgt gatgttgtag tcatattcta atttatacta aacgttattc gaatttggac
    29281 aggtatggga actgtgccta tcaatattgt aatttatgga agttgaaata gtacggttca
    29341 gtttttaaat attgttagaa attgttttaa attccctaat caatttgtat agtttttatt
    29401 tcactttatt ataactagtt tttcgcaatt tttgagacag gaatgtgatt tttgtgataa
    29461 ggttctatca ttacaagttt atttgttgta atatggaata aatcatttat atatcttttc
    29521 agaaatatgc tgtttctctg aatataaatg tctttttctt tttaacttaa ttttacaaat
    29581 tggtaaggtc atttaagact gcctttttga acagttgcta gaaatgtaga ggtattgacc
    29641 atgtatcaag atttactaag aaaaatagca gaagaaaaac caaattataa ccaggaggaa
    29701 atccagtggt tgcttgatca tttgggagat ccttctccag aaattcgtga tgatcttgtt
    29761 tttacaagct ttgctagagg gattcaggaa gagctattta cacaggagca atttcatttc
    29821 attgctgagg tggtcttagc tgatggaggg ttagataaag agattgataa ggtaggcttg
    29881 tcaacacttg aacgttcttt tagggcactt gtttatgcaa atctcttgtc tgtagatgct
    29941 aaccagcaat cggtttttta tcagggatta caatcagaaa ttcgtaatgt ccttttaaat
    30001 caaggcttgc actatctttc aaaagaaaag gatacaacag gtttctcaag tcagtatggt
    30061 tgggttcacg cttttgcaca tggagccgat ttactgaaag aggtggtttg tcatccagac
    30121 tttcctaaaa acagagttca tgaagtattt gatatacttg gccaactatt taaaagaatg
    30181 tcgattcgct ttacagatga tgaggattgg cgtttagcaa gagtgatcta tgaacctatt
    30241 ttacaaggga agttagcgca agaacaagta gcttcttgga taaaaactgt tgattttccg
    30301 atagaagaaa gggaggattt ttacaaattt tccaacttca gaacctgtct gttggaagtc
    30361 tatgtccaac ttgaccagag aaatagttta caagatgagt tgaaagaagc tatccagtct
    30421 tttcaatact agagattagc taatagttct atgattttca aaaaatttcc aatcaatttt
    30481 cttgaaaccc tttcctactt ttgatagact agtatctagt ttttgaaaaa aggagaaaga
    30541 aaatgaaaaa atttgttgct gagttaatcg gtacgttcat gcttgtattc gtcgggacag
    30601 gagctgttgt ttttggaaat ggtcttgatg gccttggtca ccttggaatc gcctttgcct
    30661 ttggtttggc aatcgtggtg gcagcctact caatcggaac tgtttcaggt gctcacttga
    30721 atccggctgt ttcgattgct atgtttgtaa acaaacgttt gtcatcttca gaacttgtaa
    30781 actacatcct tggtcaggtt gttggagctt tcatcgcttc tggcgcagtc ttcttcctct
    30841 tgggtaattc aggtatgtca actgctagtc ttggtgaaaa tgccttggca aacggtgtca
    30901 ctgtctttgg tggtttcttg tttgaagtca tcgcaacttt cttgtttgtc ttagttatca
    30961 tgactgtgac ttcagaaagt aagggcaatg gcgcgattgc tggtttggta atcggtttgt
    31021 cattgatggc gatgattctt gtcggattga agattactgg actttcagta aacccagctc
    31081 gtagcttggc accagctgtc ttggtaggcg gcgcagccct tcaacaagtt tggattttca
    31141 tccttgcacc aatcgctggt ggagttcttg cagcccttgt tgcaaaaaat ttccttggaa
    31201 cagaagaata attgaaactc aaaaagcctt gctcctcatc ttgaggaaca gggctttttc
    31261 gtatgatact cttcgaaaat ctcttcaaac cacgtcagct tcaccttgtc ctaggtatgg
    31321 ttactgactt cgtcagttct atctgcaacc tcaaaacagt gttttaagct gacttcgtca
    31381 gttctatctg ccacctcaaa cactgctttg agtaacctgc ggctaacttc ctagtttgct
    31441 ctttgatttt cattgagtat gactttagcg gttgtcaatt ttctctggat aaaggtcgtg
    31501 ttggaagagg cgttgttctg ccaagccctc atacttagtt ccttgcttac cgtagttata
    31561 gtaggggtcg attgaaatgc caccgcgcgg agtgaatttt ccccagactt ctaaatagcg
    31621 agggtctagc aagttgacca agtctttccc gatggtgttg atacagtttt cgtggaaatc
    31681 tccgtgattt cggtagctaa atagatatag tttgagggat tttgactcga cacagagctt
    31741 gtcaggaatg taggaaatat gaatcgtcgc aaagtctggc tgagcagtga tttgtcccag
    31801 cagagacata tcgaggatat ggtgacgaat gccctgttcc ttagcgattt ctctagtaat
    31861 ttgaatttcg aggtgatgac gttggccgta ggcaaaggtg acagcttcga ctgtttcata
    31921 gtgttgcatg gcccagaaaa ggcaggttgt tgaatcttga ccaccactaa agacgaccaa
    31981 ggctgattga cgtttcatag tactccttcc aaaatgggaa atgttcagag cacgcaaaaa
    32041 gctcccatta gggagctaaa aaataccaaa tcgagttttt ttagcgatgg catatcccaa
    32101 acatcgtaat attctactta tatagtaaaa tgaaataaga acaggacaaa tcgatcagga
    32161 cagtcaaatc gatttctaac aatgttttag aagtagaggt gtactattct agtttcaatc
    32221 tactatagtc tagcatattt tttgaaaaat ggcaaagagc aagaaaaaag agaccaaaga
    32281 aagtacttgg tctctcgttt gattagctca attcagcaat gatggccttg atttgttctg
    32341 ctgtgtgaac acctgcaact tgtttgacaa cttggccgtc ttttttgaag agaagagttg
    32401 gaatagacat gattccaaaa gcacgagctg tgtttggatt ttcatcaacg tccattttaa
    32461 cgattttcaa gacatcttct gaaagttctt cagacaattt gtccaagatt ggaccttgca
    32521 tacgacatgg accacaccaa gttgcccaga agtctactaa gaccaaaccg tcttttgttt
    32581 cttgttcgaa tgttgcatct gtaattgctt ttgccattgt atttctcctt tttttagtta
    32641 tattggctta aatcttgttt catgagatag aagaagatat ctccataagt cccatggtag
    32701 tccaaattat gacccttgta agttaatttt tggacagggt agtagtctgc gacgccgata
    32761 aggcaagctt gttgcgaacg ttcaaagtct tcataagact cgaaagtcac agttctttcg
    32821 ttcttgctgg catctatata ggtaatttca atcatattta aaactccttt gtttaatgct
    32881 aactttattt tactccttat aaaagagaat gtcaagaaaa atgattgcgc acgcaacttt
    32941 ttttaaaatc atcttaaatc aagaaatcca aacctgcttc caagctttct tcgacagtct
    33001 tttgtagcga ggccagtgtc ttttgcccat catttgtcag gcagataaaa ctagagcgtc
    33061 tatcttgatg gcaacacatg cgactgagta gaccgcaatt tttagcttcc aagcgagcca
    33121 ccatcctaga aactgcgctc gggctcagat gaagcttatc tggcaggtca atctggcgta
    33181 gagatttttc ttcagccaag tccagatagt agagcaggta gaactctttc aaggtcagac
    33241 tttgctcgct ctgttgggca atggtctctt ccaagagact ttcaatttct ttctgacgcc
    33301 gattgaagtc aaaccatttt tccaaatagg tcatagtgtc tcctttcttt ttagagtcat
    33361 aaatagaaga aagtccatta acgggcagtc tctgcgtcac aagatgattg cgcatgcaat
    33421 aattatacta cttttcaaga atactggcaa gctctgtttt ttagtggttt tcttttttac
    33481 tgtctatatt tttggtaaaa atcaactttt acttggatga aggttctggc ttcacgtagg
    33541 agttgaagaa gagtggcgcg ggtttcaaac tcttctcttg tcttgggcag actgcggttc
    33601 cggaagactt ccagataacg ttcaatttca tctagcaaat cagaagcagg attggtctgg
    33661 ctcagttgac ctgcaatttt tgaaaagagt tgcgctaaga tcaggctttc actggcggca
    33721 aggtgacaag tgttaatctg ttgggccatg tttctcagga tacgactttg tcgttgtctc
    33781 atctcaaagt agtggatatg gtagtcggtc tggtgaaaga ggtggtcaga gtgatccaaa
    33841 tagaccagtc tgagggcttc tttcaaaagc gtgtctaatt ctgctaccag ctgtgctcgg
    33901 ttgcgtccgt ctcctctgga taaatagtat ttgaagcgct ggaggatatc ttttaacttt
    33961 tcttccacca gcgtgtggta gtgctggatt tcctcttctc gtgaaggcat atagagatta
    34021 acaagcaagg caaatcctgt accaatagca aagagaagga attcgttgac taggaggtct
    34081 ggagaggttg actcttgaac caagagatgg ctaaccaaaa ccgtgcttgg tgtgatgcca
    34141 atttcccagc ccatcttgta ggctaaagga acgtagaagg ccagatagag gccgagactc
    34201 cagatatgaa atccgctcaa gtgaaaagct agaacgccga tagccagagc tagaagcata
    34261 gaaaaaagac gattgcgagc cagttttaaa gtacttctac gcgtatcaga taggctcaag
    34321 agagcgataa ttccagccga aactgctgac gaaagattga gaaaataagc aagcaggcag
    34381 gcaagacagg tagctaagat gagcttggtc gtacgttggc taatagacat aagaatttcc
    34441 taataagtta gaataaaagc gtaaaagaca agacatgagc aggcttgcct tgatgagtta
    34501 ttttttacgg gttgctgcgt attcggcaac ggcggtaaag aggacatctg tagaagagtt
    34561 aagggctgtt tcacatgagt cttggatgac accaatcaca aaaccaaccc caacaatttg
    34621 tatggcaata tcgttagaaa taccgaaaag gctacaagca actgggataa gaaggaggga
    34681 acctccggca atacctgaag catcacaggc tgagatagct gctaccacac tgaggacaaa
    34741 ggctgtggca aagtcaacag gaattccaag agtgttaact gtaacaaggg tcaaaacgtt
    34801 aatggtaatc gctactccag ccatattgat agtagaaccg agtgggatag aaacagaata
    34861 ggtatctggg ttgagtccaa ggtcatggca gagtttcatg ttgacaggaa tgttagtcgc
    34921 agaactacga gtgaaaaagg ctgtcacacc gctgacacgg aggcagttcc aaactagagg
    34981 gtaaggattg cgtctcataa agaagaaggc aatcaaaggg ttgaccacag gggcaacaaa
    35041 aagcatagtc gttactaata gaaccaataa aataccgtag ttggcaaggc ttccgactcc
    35101 cttgtcagaa atggttttaa aaacaagacc aaggattcca aatggagcca gattgatgat
    35161 ccattcgaca attttagaag tcacgtcagc gatagttttt agcaattctt tactattttt
    35221 actggcttct ctcatagcga ttccaaaaat gactgcccaa gataagattc taatatagtt
    35281 agcagtaagc agggcgttga ctgggttgtc aaccagtttg agcaagaggt tgctgaggac
    35341 ctgcccaatc ccatctggtg gtgcaatttc agtattggca ctatttaggg taatttcaat
    35401 agggacgatg aaacttgcta gtacagctac aagagcagcg gcgaaagtcc ctatcatagg
    35461 atatacaaga aaacaacagt tttcatattg ctatcttgtc ccttttgatg ttggaaaggg
    35521 cattggcaac gagggcaaag actaggatag gagcaacagc ttttaaacct ccaacgaata
    35581 aatccccgag tagcccaatc cctgagagat taggaagggt cagtcctagg attccccaca
    35641 aagcatacca atcaagatac gcttgacaag gcttgcctta ttccaagcat gaatgattct
    35701 tttcataata atctcctttt tgtgtagtga ttatgattat agtataaatg atagacaaaa
    35761 tcaagaattt tctgtctatt ttttgaatat ttatggagaa tgagactgat gaaaatatgg
    35821 tataatgaaa taaaggagtt ttatatgcaa aaatttattc aggcttatat tgaaaagcta
    35881 gatgtgacaa ccattatcga gaatattcta accaaggtca tttctctttt actgcttttg
    35941 attgtatttt atattgctaa aaaaatgctt cataccatgg tgcagagaat tgtcaaacct
    36001 tctctaaaaa tgtctcgtca tgatgtcgga cgccaaaaaa ccatctcacg tttactagaa
    36061 aatgtgttta attatacgct atatttcttt ttactctact gcattttgtc gattttaggt
    36121 ttgccagttt ctagtttgct ggctggagct ggtattgctg gggtagcgat tggtatggga
    36181 gcccaaggct ttctgtctga tgtcatcaat ggctttttca tcctctttga acgtcaactg
    36241 gatgtgggag atgaggtcgt tctgacaaat ggaccgatta ctgtatcggg taaggttgtc
    36301 agtgtgggaa ttcgtacgac acagcttcgt agcgaggagc aagcccttca ctttgtccct
    36361 aaccgaaata tcacagttgt tagcaatttc tcacgcacag actagacctg ttattttaag
    36421 taatttgtgg tacaatagag ggagtttaat aaggagaaaa gatggtttta gaaaagcagt
    36481 tgggcaatgg ttgtacctgg atagacctag acctaggaaa gttgaataaa ctagaagacc
    36541 tttctgaaat ttacggtttg gacaaggaaa ccattgaata cgcactggat agaaacgagc
    36601 gcgcccacat ggattaccac cgtgaaagtg ggacggttac ctttatctat aatgtcctag
    36661 acgtaaaaaa ggacaaggcc tactatgaga cttttcccat gacctttatt gtcgagcatc
    36721 gtcgcctgat taccattagt aataccaaga acgcctatgt cattgaacag atgactcgtt
    36781 atctggagag ccatgacacg ctttcgattt ataagtttct ctttgccagt ctggaaatta
    36841 tcagcaatgc ctactatcct gtcattgagc agatggacaa gagtagggat gaggtcaatg
    36901 acctcttgcg ccagcgaact accaagaaaa acctctttgc cctgtctgat ttggagactg
    36961 gtatggttta tctgacggca gctgccaaac aaaatcggat tttgttagag catattcaag
    37021 gtcatgcctt gtatcgtagt tttgatgaga ttgagagaga acagtttgat gatgccatga
    37081 ttgaggctca tcagctggta tccatgacag acctaatctc tcagatttta cagcagcttt
    37141 cagcctctta caacaatatt ctaaacaata atctgaatga caatttgaca accttgacta
    37201 tcatttcagt cttgctagct gttttggcag tcgtgacagg ctttttcgga atgaatgttc
    37261 ccttaccttt aacagatgag ccccatgctt ggctctatat cagtttggct agtgcaggtt
    37321 tgtggattgt tttatccttg ttactaagga aaattgcgaa aaaaagttaa gaaaaggagc
    37381 cagaatggcg attgaaaatt atataccaga ttttgctgtg gaagcagtct atgatctgac
    37441 agtcccaagc ctgcaggcgc agggaatcaa ggctgttttg gtcgatttgg ataataccct
    37501 cattgcttgg aacaaccctg atggaacgcc agagatgaag caatggctac atgaccttcg
    37561 ggacgcgggt attggcatta tcgtagtgtc aaataacacc aaaaaacgcg ttcaacgagc
    37621 agttgagaaa tttgggattg attacgttta ctgggccttg aagcccttca catttggtat
    37681 tgaccgtgct atgaaggaat tccactatga caaaaaggaa gtggtcatgg ttggcgacca
    37741 gctcatgaca gatatacgag cagcccaccg tgcagggatt cggtcaattt tagtcaaacc
    37801 cttggtccaa catgactcaa tcaaaacgca gattaaccga actcgtgagc gtcgtgttat
    37861 gagaaaaatc actgaaaagt acggaccgat tacatataaa aaaggaattt aactatggaa
    37921 gaaattctct gtattggttg tggagcaacc attcagacga cagataaagc tggtcttggt
    37981 tttacccccc agtcggcact tgaaaaaggt ttggagactg gcgaagtcta ttgccaacgc
    38041 tgtttccgtc tccgccacta caatgaaatc acagatgtcc agttgacgaa cgatgatttc
    38101 ctcaagctct tgcacgaggt gggagacagt gatgccttag tggtcaatgt cattgatatc
    38161 tttgatttta atggatctgt catcccaggt ttaccacgtt tcgtctcggg caatgatgtc
    38221 ctcttggtag gaaataaaaa agatatcctt cctaagtcag ttaagtctgg taagattagc
    38281 cagtggctca tgaaacgtgc ccatgaagaa ggtcttcgtc cagtcgatgt ggtcctaact
    38341 tcagcacaaa ataaacatgc cattaaggaa gtcattgaca agattgaaca ctaccgtaag
    38401 ggccgcgatg tctatgtggt cggtgtgacc aacgttggaa aatcaactct aatcaatgct
    38461 attatccaag aaatcacggg tgatcagaat gtcatcacta cttcacgctt cccaggtaca
    38521 accttggaca aaatagagat tccgcttgac gacggatctt atatttacga tacgccggga
    38581 attatccacc gccaccagat ggctcactac ttgacggcca aaaacctcaa gtatgtcagt
    38641 cctaaaaagg aaatcaagcc taagacctat cagcttaatc ctgagcaaac cctattttta
    38701 ggtggtttgg gacgctttga ctttatagca ggagaaaagc aaggatttac tgctttcttt
    38761 gataatgaac tcaaactcca tcgtagcaag tttgaaggag ctagtgcttt ctacgataag
    38821 cacctgggaa ctcttctgac accaccaaat agcaaggaaa aagaagattt cccaaggcta
    38881 gtccagcatg tctttaccat taaagataag acagacctag tcatctcagg cctaggctgg
    38941 attcgtgtaa caggcatagc aaaagtcgcc gtctgggcac cagaaggcgt cgccgtcgtc
    39001 acacgaaaag caattattta aaacaaaatc gctgagcgaa caaagtgagc tcatatagcg
    39061 atagttattg ccgtggtgtg attgccaatc tgtagattgg caaggggcac aattgagacc
    39121 aaggctcaat tgtgaggtca ggaaatccat ttttcaaaga tgaaatcttt ggaaaattag
    39181 ttccgaccgt ccctcaccac ctaaaataac tatccaaaaa caaggaagaa aattatgtca
    39241 ttaacatcaa aacaacgtgc cttcctcaac agccaggcac acaccctcaa acctatcatc
    39301 caaatcggga aaaatggact caacgaccaa atcaaaacca gcgtccgtca agctcttgat
    39361 gcgcgtgaat taatcaaggt tactctctta caaaacacag atgaaaatat ccacgaagta
    39421 gctgaaattt tggaagaaga aatcggtgtg gatacagtcc aaaaaatagg acgcatcttg
    39481 attttgttta aacaatctag caagaaagaa aatcgcaaga tttctaagaa agtcaaagaa
    39541 atctaagacc gaaactccaa ataactgttt ttatagagaa ataaagggga ctagcctatg
    39601 acaatcgaac tattgactcc ctttaccaag gtagagttgg agccagaaat caaggagaaa
    39661 aaacgcaaac aagttgggat tttagggggg aattttaacc ctgttcacaa tgcccatctc
    39721 attgttgcgg atcaagtacg gcaacagttg ggactggatc aagttcttct catgcctgaa
    39781 taccaacctc ctcacgttga taaaaaggaa accatccctg aacaccatcg tctcaagatg
    39841 cttgagttgg caattgaggg gattgacggc ctagtcattg aaaccattga gttggagcgc
    39901 aagggtattt cctacaccta cgacaccatg aaaatcttga cagagcaaca tccagatacg
    39961 gattattact ttatcatcgg tgccgacatg gttgactatc tgcctaagtg gtaccgaatt
    40021 gatgaactgg ttgacatggt tcagtttgtg ggggttcagc gtccacgcta caaggcaggg
    40081 acttcctatc cagttatctg ggtggacgta ccgctcatgg atatctcgtc cagcatggtg
    40141 cgggacttca ttgcccaagg tcggaaaccc aactttctcc tacctcagcc agtgctagac
    40201 tacatcgaga aggaggggct ctactgatgt cctatcaaga ctatatcaac tgctcccgtg
    40261 aggccttgtt ggaaaaaatg gcagagcttc tacctgagaa acgtctaacc cattgtctag
    40321 gtgtggagcg tgccgctatg gagttagctc agcgctttgg tgtagatgtt gagaaagcaa
    40381 gtctagcagg tcttcttcat gactatgcta aaaagttgtc agatcaggaa tttctagact
    40441 tgattgaccg ttatcagcta gatttcgacc tcaaaaactg gggtaataat gtctggcatg
    40501 gtatggtcgg aatttacaaa attcaggaag atttggattt gcatgattca gaaatcctgc
    40561 gagccattga agtccataca gtcggtgctg gtcaaatgac agacctagat aaggtcatct
    40621 acgtcgcaga ctatatcgag cacaatcgag cctttcctgg agtagatgtg gcgcgtgaga
    40681 ttgcaagtct atcgctcaat aaggcggtgg cctacgaaac agctcgtacc gtggagtatc
    40741 tagctcatca gggattcccc atctatcccc aaacccttga aacctataac gcctttgtgc
    40801 actatttgaa agaggactaa atgaagacgg cttttataat cattgatgtt cagaatgttt
    40861 tagtagaaac tgggttccag acaaaaagtc tattggaaaa aatttcttat ttacaaaacc
    40921 aggctagaag caagaatatt gaaattatct atgttcaaca tattgagaat tctgaagctc
    40981 aaacatcaga agattggcag ttatctgcgc ttttaaatcg aaaacctgct gaaaaggtct
    41041 ttcagaagaa gtataacagt attttcaaag aaactggctt aaaagaatac ttggataaac
    41101 aggggattga aaaattagtt ttatgtggta tgcagacaga atattgtgtg gatacctctg
    41161 tcaaggttgc ctttgaatat ggctatcagc ttattgttcc agaaggtgct gtcacaacct
    41221 ttgatgggga tgacattcca gcagaaacga ttaatgaatt ttatgaggac atttgggagg
    41281 agtgctttgc agatgttcta gattacaaac atattttcta aaaagaggat taaatgaacg
    41341 aaaaagaatt actagaacta gtcgtgaaag cggctgatga gaaacgtgcg gaggatatcc
    41401 tcgcacttga tgtacaagat ttgactagtg tgacggacta ctttgtcatc actagctcaa
    41461 tgaatagccg tcagttggac gctatcgcag ctaatatccg tgaaaaagta gctcaagcag
    41521 gctttaaagg tagccatatc gaaggcgatg cagctggagg ctgggtctta ctggacctcg
    41581 gtgctgttgt cgtgcatatc ttttcagaag aaatgcgtgc ccattataat ctagagaagc
    41641 tatggcatga ggcgaattca gtagatattt cagaagctct tgcttagaag atgaactcag
    41701 ttgtagtcaa ctgggtttct ttgcttattt tagaaaaatt gatagaaagg taaagggcta
    41761 gtggtgttta ccatggagct cttagtatca tgattatggc gacttatgaa acctttgcgg
    41821 cggtctatga tgcggtcatg gacgatagtt tatacgacaa atggacgaat ttttctctgc
    41881 gtcatttgcc taagaccaag gagagaaaga aactcttgga attggcttgt gggacaggaa
    41941 ttcaatcagt gcgcttctct caggctggtt ttgatgtgac tggacttgac ttgagtgcgg
    42001 atatgttgaa gattgcggag aagagagcaa cttcagccaa gcaaaagatt gcttttatag
    42061 aaggtaatat gctgaatttg tccaaggcag ggaaatacga ttttgtcacg tgttattcgg
    42121 actctatctg ctatatgcag gatgaggtgg aagtagggga cgtctttaag gacgtgtaca
    42181 atgcgctgaa tgaagaggga gttttcatct ttgacgtgca ctcgacctac cagacagatg
    42241 aagtattccc tggctattcc taccatgaaa atgcggaaga ttttgccatg ctttgggata
    42301 catatgagga cgaagttcct cactccatcg tccatgagct gactttcttt atcaaggagg
    42361 ctgatggttc ctttagtcgc cacgatgaag tacatgagga gaggacctat gagatcttga
    42421 cctatgagat cttgctggaa caggctggat tcaagtcctt caaactctat gcagactttg
    42481 aggataagga gccaacagaa actagcaccc gttggttttt tgtggcgcag aagtaggaga
    42541 ttagcatgac cataacaggt attatcgcgg agttcaatcc ttttcataat ggccataaat
    42601 acctgctgga tcaggcagag ggactgaaaa ttgttgccat gtcagggaat ttcatgcagc
    42661 gtggagagcc tgctattgtg gataagtgga caagggccca gatggcgctg gaaaatggcg
    42721 cagacttggt agtggaattg ccctttttag tcagtgttca ggcagctgat ttctttggtc
    42781 aaggagctgt ggatatcttg gatcgcttgg gcattgatag tctagttttt gggacagagg
    42841 aagttctgga ttaccagaaa atcgctgact tatacacaga gaaaggtgct gagatggaga
    42901 aatttgtgaa aaatctgcct gattcccttt cctatcccca gaaaacccaa gctatgtgga
    42961 aggaatttgc tggtcttgat ttttcaggta atacccccaa tcatgttctt gctctggcct
    43021 atgcaaaggc ggttgcagga cgaaatatca agctgcatcc gattcagcgt cagggggcag
    43081 gttaccattc tgtgaataaa gatgtggact ttgcctcggc gacagccctc cgtcaacatc
    43141 agaaggacca agatttctta gaacgtttta tgccttctgt tgccctattt gagcaggcca
    43201 gtaaggtgat ctgggaagac tattttccct tgctccgcta tcaaatcttg tcaaatcccg
    43261 acctaaccac catctatcag gtcaatcaag aaatggctgt gcgcattaaa gaagctatta
    43321 aaacagccca gtctgtggaa gaattggtcg agttagtgac taccaaacgt tacaccaagg
    43381 cgcgtgtcag acgcctattg acctatattt tggtacaggc tagagaaaat gtcttgccag
    43441 aagccattca tgtccttgga tttactgaaa aaggcaggca acatctcaag tctctgaaag
    43501 gacaggtcaa tctagtcagc cgaattggca aagaaccttg ggatgctatg actcaaaagg
    43561 ttgaccagat ttaccaacta ggaaagccaa gtatagcaga gcaaaatttt ggcagagtgc
    43621 cgattagaat agaaacaaac taagtctact gaaaggtagg cttttttatt gtagaaattt
    43681 tagataacaa attgaagata aagtttttgg aaaatgataa actccctaat acagaagaca
    43741 aaaagtcaat aaagaaaatt agtatatgaa ccttaatctt tatctttgaa tccgctttat
    43801 gcttgttatt ttgtggtata atgaagagaa ctggttatat taataatcat aaattgaata
    43861 taaaagacaa ataaattttt atatttataa accatgataa acttttgttt aagattatgt
    43921 aataaaaaag aagatttata aggataattt gtatttatgt agtacaagct aatgtaggat
    43981 agttaaaata tggaagaaaa ttataattta tctataactc aaataaagaa ttctattaaa
    44041 gaaaattcct tagttttgtt tgttggtgca ggaatatcag ctaattctaa tctaccaact
    44101 tggggagaat tgattcaatc attaaaaaaa gaattgaaca taccagaaga aagaacagat
    44161 agtccactga gaattgctca gtattattat gatacttttg ggaaaaatca gtacacaaag
    44221 aagatagaag aaattttctt taagaaggga ttgagtaaac caaatgagtt acataaatta
    44281 atagaaaaaa ttgcaccaaa acatattatt acgacaaact atgattcact tctggagagt
    44341 caatttgaaa gtggcttgtt aaagtacaat gtagtcgctg aggataaaga catcccttat
    44401 acgagttcgg aaagatactt gattaaaatg catggtgact tcagtaagaa aaatattgtt
    44461 ttgaaagaag atgattattt agactatcat ttgaacttcc caatgatttc tactttaatc
    44521 cagtcattga taatgaatca tactctattg tttgtggggt attctctaag tgattcgact
    44581 tttaattcaa tttttagaat gattcaaaat acatttaaat tggatgctaa aaatgcttat
    44641 ttttatactc ccgaggaacc gtctatgatc attcgagagt attataagaa gcaaggaatt
    44701 ttcattatct ctaatgaaga gaatctaggt caggagacat cagaaaaaca gaacaaattg
    44761 tattgcagga caaaagattt tttagaggtt ctatcagaaa accgaagtca agatgtaaat
    44821 aatgctgatg atttatggaa tcaactggct tttttagata gacttagttt tattgatgcg
    44881 aaagattttt ctagatattc tgatttaaag aaaagggctt taaactggga tgatgagtat
    44941 tgctggtttg gaaatgatca attaagattt gaaattgatg gtcatgaaga attacgaatt
    45001 atggtatcta aaaaatcttt attaaatcgc tttctagata tggagattgg tgagcctagg
    45061 gatttaaagg ggaatcgatt cttaagtaag gcttttaagt tgtatgaaga aaaacaatat
    45121 tctttagcaa aagcaaaatt tagagaacta gctaatatag catttcgtca gaaagactat
    45181 tttaatttct tagtttgtga atttaatttt cagcaaattc aaataattga tagatacgaa
    45241 aaaacaccta gttatgcaga gccactttat gatggagaat taagcgaatt aacagaacaa
    45301 attcttaact cggttacagg agatgagaaa aagattatag aattttttag agattcaatc
    45361 cttaacttca attttttata tagaaaatta gaaactatta atgaattatt tgatgaaatt
    45421 cgtgaagagc atgaaagcta tagaagagga ggatggtctg ctaataatca tctatttaat
    45481 gcggagttta cagtaaaaaa tctttataac tttttaaaat taaattgttt atgtgttgaa
    45541 cattataaga tttataaatc aatcattaat agatatttag aaatattatt actcagttat
    45601 gataattcat atgtaaaccc tgactcaagt attttcgata gaacatcttc ttggcttaaa
    45661 aatcttgatt tggatgatgt taaattaatt cttccgaata tagattttaa ggttgtaaat
    45721 ttatatttta gaaactattc atttggtaaa ataaaagtta ctgaggaagc gaaagactac
    45781 ttgttgaatc gaattactta tcttcaagaa agacttgaaa ttactgaaga tgaaaatctt
    45841 cgagagctta aaaatatgct aatttttctt ccccttgttg atgatattga catagaaaaa
    45901 gtaatagaaa ttttaaataa tcaaacactg tattataatt ggagcgagga atttaggaga
    45961 attattaaaa ttgtcctcga taatatggat gttattgata aagattcatt gaaatctaag
    46021 ataattggga ttgtaaataa acatttaaat gaaattttgg agaaaaattt ttctttatat
    46081 aattcagtgt atccgctcta ttcacagtta ctagaatact gctcaactga tcaggaaact
    46141 gcaataatag tactcgaaaa atttaataca gatattttaa gaataaaata taaaaatgat
    46201 gatataaaaa atatcataga atattctgac ttgatatgtc acttatttaa gtattttgaa
    46261 aaggatatta aagatgacat tttagatact ctaaaagttt atgaagaatc agaaaatatt
    46321 atttaccata aagtaattga tttgatgagt tataatgtct atgattttcc tcagatacaa
    46381 aataaaatct accattattt gattaaaaga attaatgata aaagagttga gggggttaaa
    46441 actttcccag acccaagaga aaagtctgta tcagatttat acaacttaag tagaaaagga
    46501 tatttttctg attttgagat tttaaaagat attgaagaag atattcgagg actttatcct
    46561 gaagtagatt ggacctggtt ccatgataga agtgatgatg ttatccatcg tctactagaa
    46621 catagaactc ccaataatat taaaacttat ttttcaaaaa atgaagaaga taataaattg
    46681 attaatgagt atattttaaa agctttcgat gaggataaat taatacttaa gaaataaatt
    46741 gatacaacta aataaaaaag ttaatttatg acaaatttga tcttgaagat aagctgtgat
    46801 ttcctaaagt gaattttcaa agacttgaaa gcaaaatgtt tgaatctagt ggaacactat
    46861 ttggcacaac aatagaaaat ccatagcggt agaaattcgg tggaactttt tgatagcggc
    46921 actgacttat ctccttaaaa aaccaaggaa gtgtaatcct aaaattcaga ctaatttccc
    46981 caaaacatat tctattttag gtttgtgaaa atcacttttt tatggtagaa tgtaaaagaa
    47041 tgtatgtcat tcggaataac aataaaatga ggtaaaaaca tggagatcat gtcgcttgcg
    47101 attgctgttt ttgccgtcat cattggttta gtcattggat atgtcagcat ctcagctaag
    47161 atgaaatcat ctcaggaagc tgcggagttg atgcttttaa atgctgaaca agaagcaact
    47221 aatttacgtg gacaagctga gcgtgaagcg gatttacttg ttaatgaagc taaacgtgaa
    47281 agcaagtctc ttaaaaaaga agcactattg gaggccaaag aagaagccag aaaataccgt
    47341 gaagaagtgg acgctgaatt caaatcagaa cgtcaagaac tcaaacaaat cgaaagtcgt
    47401 ttgacagaga gagctactag ccttgaccgt aaggacgaca atttgacgag taaagaacaa
    47461 acacttgaac aaaaagaaca aagtatttct gatagagcga aaaaccttga tgcgcgtgaa
    47521 gagcaattag aggaagtcga aagacaaaaa gaagcagaac tagagcgtat tggtgctctg
    47581 tctcaggcag aagcacgaga tattatcttg gctcagacag aggaaaactt gaccagggag
    47641 attgccagtc gcattcgcga agctgagcaa gaggtcaagg aacgttctga caaaatggcc
    47701 aaggatatct tggttcaagc tatgcaacgt atcgctggtg aatatgtagc ggagtcaaca
    47761 aactcaacag ttcatctgcc agacgatact atgaagggac gcattattgg tcgtgaaggt
    47821 cgtaacattc gtacctttga aagtttgaca ggggtcgatg tgattatcga cgatacacca
    47881 gaagtggtga ccttgtcagg atttgatccg attcgtcgtg agattgcccg tatgactatg
    47941 gaaatgttgc tcaaagatgg tcgtatacat ccagctcgta tcgaagagtt ggttgagaaa
    48001 aaccgtcaag agattgacaa taagattcgt gaatacggtg aggctgctgc ctatgaaatt
    48061 ggtgcgccaa accttcatcc agacttgatg aagattatgg gacgtttgca gttccgtact
    48121 tcatatggac aaaatgtttt gcgccattcg attgaggttg ctaagttggc tggtatcatg
    48181 gcgagcgaac ttggtgaaaa tgcggctctt gcccgtcgtg ctggattcct tcacgatatc
    48241 gggaaagcca ttgaccatga ggttgaaggt agccacgttg aaatcggtat ggaattggcc
    48301 cgtaagtaca aggaaccccc agttgtggtg aatacgattg ctagtcacca cggagatgtt
    48361 gaagctgaga gcgtgatagc agttatcgtc gctgcagcag atgccttgag cgcagcccgt
    48421 ccaggtgctc gtagtgagtc tcttgaaagc tacatcaagc gtctccatga tttggaagaa
    48481 attgctaacg gctttgaagg agtgcaaact agctttgccc ttcaagcagg acgtgaaatt
    48541 cgtatcatgg tcaatccagg aaaaatcaag gacgacaaag tcacaatctt ggctcacaaa
    48601 gttcgtaaga aaattgaaaa caatctcgat tatccaggaa atatcaaggt aaccgtgatt
    48661 cgcgagcttc gtgcagtaga ttatgctaaa taaataagaa agagcagttg aaaaattact
    48721 gcttttttgt tacactagat agaaagactg taaaggataa tctggctttt tgccattatt
    48781 ctagagaaat gttatttcac agattgacta aaaggagaca caatggcaga ccgaggctta
    48841 ctaatcgttt tttctggtcc ttcaggggtt ggaaaaggaa cggttagaag agagattttt
    48901 gagagttctg aaaaccaatt tcaatattct gtatcgatga cgacacgcgc acaacgtcct
    48961 ggagaagtgg acggtgttga ctatttcttc cgtactcgtg aagaatttga agagctgatt
    49021 cgtcaaggac agatgttgga atacgcagaa tatgtcggca actactatgg aactcctctg
    49081 acctatgtca atgaaacctt ggacaaggga atcgatgttt tccttgaaat tgaagttcag
    49141 ggtgctcttc aggtcaagaa aaaggttcca gatgctgtct ttatcttcct gacaccacca
    49201 gatttggatg aattgcaaga tcgcttggta ggtcgtggaa cagatagtgc agaagtgatt
    49261 gcccaacgaa tcgaaaaggc caaggaagaa attgccctca tgcgtgagta tgattatgcg
    49321 attgtcaacg atcaggtgcc cctagctgct gaacgtgtca aatgtgtgat tgaagcagaa
    49381 cacttctgtg tggatcgtgt cattggtcac tatcaggaga tgttaccaaa atctccaact
    49441 acccgataaa atttagaaaa taggtacaag caaatgatgt taaaaccctc tattgatacc
    49501 ttgctcgaca aggttccttc aaaatattca ctcgtaatct tggaagcaaa acgtgcccac
    49561 gaattggaag caggtgcccc agcaactcaa ggtttcaagt ctgaaaaatc aactcttcgc
    49621 gctttagaag aaatcgaatc aggaaacgtt acaattcacc cagatccaga aggaaaacgt
    49681 gaagcagtgc gtcgccgtat cgaagaagag aaacgccgca aagaagaaga agaaaagaaa
    49741 atcaaagagc aaattgctaa agaaaaagaa gatggtgaaa aaatttaagg ttggggggac
    49801 tcaatcttat tttttctatt gcaagaatgt actgacaagg aggaggtgag aagatggccc
    49861 tagctaagat tatcgtagat gtgcctttga tgcagacgga ccagccctat agttacagga
    49921 ttccggagga atttgaggga atgctggaag ttgggatgcg ggttcatgta ccttttggta
    49981 agggtaatcg cctgattcaa gggattgttc ttggtttgga gtcccaatca gatggagaag
    50041 agatggagca agatttaaaa gatattgcag aggtgctgga tttttctcct gttctcacgc
    50101 cagaacaact ctggctggct gaggagttac gcaaatctgt cttctcctac aaaatctcta
    50161 tcctcaagac catgcttcca ggatttctga attctagcta tgacaagatt ctctatcctc
    50221 tggaaggctt gagtcaggaa gaacgagtac gcctgtttgg ttcagaagat tcgctagcct
    50281 tttcttcact agaccttgcc aagcaagctg aaatgatgcg tttgactaga aaaggcctgc
    50341 ttggtctgga atatcaggca gtcgatcaaa agaaggtcaa gacccagtct tggtatgagg
    50401 ttgaccatgc ccaattagaa ggtgttgaga tttctacacg tgccaagaaa aagttggaac
    50461 tgagagatta tctgctgtct catccagaga gcgcttcctt ggctagcttg ttagagtcct
    50521 actcgcgaga gcaagtcaac ttctttgtgg atcaaggtgc tgttaccata gtccaaaagg
    50581 aagttcaacg ctcggctgct tattttgaag gaattgaagc aagtagacct ttggagttaa
    50641 atccagaaca aagacaggcg cgtgatgcgg ttgtgagttc tattggcagt tctcaacctc
    50701 cctttctact tcaagggatt acaggaagtg ggaagaccga ggtttacttg cagattatcc
    50761 aaggtgccct ggataagggc aagacagcta ttttgctggt acctgagatt tccttgactc
    50821 ctcagatgac cgagcgtttt atagcacgtt ttggggacaa ggtagccatt cttcactcag
    50881 gattatccaa tggtgaaaag tatgatgaat ggcgcaaggt ggagcgtggc gatgcccaag
    50941 ttgttgttgg tgccagatct gctatctttg ctcctctgaa aaatctgggt gtcatgatta
    51001 ttgatgaaga gcatgaagcg gcttataagc aggacagcaa tccccgttac catgccagag
    51061 aggtagctat tttacgggct cagtataatc aagcaactct ggtacttgga tctgcaacgc
    51121 cgagcttaga gagccgggca cgtgctggca aaggcgtcta tcaacactta cgtctaaccc
    51181 aacgtgccaa tcctttggct acaatccctg aggttcaagt gattgacttt cgagactata
    51241 tcggacaaaa tgagacgtca aactttacgc ctcctttgct agaggctatc caagaccgtc
    51301 tggttaaaaa agagcaggtg gttctcatgc tcaatcgccg tggttattct agctttgtca
    51361 tgtgtcggga gtgtgggacg gtggatactt gtcccaactg cgatatttct ctgaccttgc
    51421 atatggatac caagactatg aactgccatt attgtggttt ctcgaaggat attcctcagg
    51481 tctgtcctaa ctgtaagagc cacagtattc gttactatgg gacgggaact cagaaggctt
    51541 atgatgagct agcagaactc tttccccaag ctcgcatttt taggatggat gtggatacga
    51601 ctcgaaagaa aggcagtcac caagccttgc ttgaccagtt tgggcgaggg gaagcggata
    51661 ttttacttgg tactcagatg attgcaaagg gtttggattt tcccaatgtt accctagtcg
    51721 gagttctaaa tgcggatacg gccttgaatc tgcctgattt ccgttcttct gagagaacct
    51781 tccagctctt gactcaggta gcaggtcgag caggtcgggc tgaaaaagca ggtcaagtct
    51841 tgatacagtc ctacaattcg cagcactatg ctattcgatt tgccaaggat caagattacg
    51901 aaggctttta tgcctatgaa atgggaatca gacgacaact cggctatcca ccttactatt
    51961 tcacgattgg tattaccctt tctcacaaga aagaagaaga ggttttcaaa cgtgcctatg
    52021 aagtcatgaa cattttgcgg tcaggcttgt cagagaccag tcccattctg gggccaacgc
    52081 caaaaccgat tgcccgtacc cacaacctct atcattacca gattttaatt aaataccgtt
    52141 tagaagatga gctggggccg accctcaacc aggtcttggc cttgactcaa gaacgggaaa
    52201 atagtgagct ccgtctcagc attgaccatg agccgcagca atttttataa gaaggagaag
    52261 atatgacaaa actaatcttt atggggaccc ccgacttttc agcaacagtc ttaaaaggac
    52321 ttttgacaga tgaccgttac gaaattctag ccgttgtgac ccagccagac cgtgctgttg
    52381 gtcgtaaaaa agttatccaa gaaaccccag tcaagcaggc tgccaaggaa gcaggactat
    52441 ctatctacca acctgaaaaa ttatctggaa gtccagagat ggaagaactt atgaagctag
    52501 gagcagatgg aattgtgact gctgcttttg ggcagtttct cccaagcaaa ctccttgata
    52561 gcatggactt tgctgtcaac gttcatgcct ccctccttcc tagacaccgt ggtggtgcgc
    52621 ctatccatta tgccttgatt caaggggatg aggaagctgg tgtgaccatc atggaaatgg
    52681 ttaaggaaat ggatgcagga gatatgattt ctcgtcgcag cattccgatc acagatgagg
    52741 acaatgttgg caccttgttt gaaaaattgg cgctagttgg tcgtgatttg cttttggaca
    52801 ctctgcctgc ctatattgct ggtgatatca aacctgaacc gcaggatacg agtcaggtta
    52861 ccttctctcc aaatataaag ccagaggaag aaaaactgga ctggaacaaa accaatcgtc
    52921 aactctttaa ccaaattcgt ggaatgaacc cctggcctgt tgcccatact ttccttaagg
    52981 gcgaccgctt taagatttat gaagccctac cagtagaagg tcagggaaat ccaggtgaga
    53041 ttctctctat cggcaagaaa gaattgattg tcgcaacggc tgaaggggct ctatccctca
    53101 aacaagtgca gccagctggt aagcctaaga tggacattgc ttccttcctc aacggagttg
    53161 gacgtacatt gactgtagga gaacgatttg gtgactaaag tagaaacggc tagaagttta
    53221 gctctagcag tgctagagga tgtttttgtg aaccaagcat attcaaatat cgccttaaat
    53281 aaacacctca aggggagtca gctctctgca gcagacaagg gcttagtgac cgagctagtc
    53341 tatggaacgg tagcccgtaa actgactctg gaatggtacc tatcccactt tatcgaagac
    53401 agagaccagt tagacagctg gctctatgtc cttcttctca tgagtgtcta ccaactccgc
    53461 tatttggaca agattccaga tcatgctgtg gtcaatgaag cagtggaatt ggccaaactc
    53521 cgtaaaaaag gcagtgaaaa attggtcaac gctgtccttc gccgtatctt gcgtgaaggc
    53581 tggccagata ttgctaacat caagcgaaaa aacaagcgtg actccattgc ctattctctc
    53641 ccagtttggc tagttaccaa actcaaggaa gaatacggag aggagagagc aaaagccatc
    53701 tttgaaagct tgttagtgcg aaacaaagct agtattcgtg taacagacct aagtcgaaaa
    53761 gaggaaatcc aagccttgtt ggaggcgaat agttcccttt tagcaaccac tggtctggtt
    53821 aaggagcaag ggcattttgc agggcataat ttgtttgcgg atggagccat taccatccaa
    53881 gatgagtcca gtcagctggt tgctccgacg cttgatttac aaggtgatga gcgagtgctt
    53941 gatgcctgtg cggctccagg tgggaaaaca gcccatatag cctcttatct tacgacaggt
    54001 caggttactg ctctggactt gtacgaccac aagttggatt taattcaaga aaatgcccaa
    54061 cgtctgggag ttgaagatcg ggttcaaact caaaaattgg atgccagaaa ggtgcatgag
    54121 ttttttgacc aggattcctt tgataagatt ttggtggatg ctccttgttc aggaatcggt
    54181 cttttgcgcc gaaaaccaga tatcaaatac aataaagaaa cggcagattt cgcgtccttg
    54241 caggaaattc agttagaaat attaggtagt gtttgtcaaa tactaggcaa aggtggtata
    54301 ataacttata gcacctgcac tatcgtctca gaggagaatt ttcaagtcgt taaggccttt
    54361 ttagagagtc atcccgagtt cgagcaggta aaactagaac atgaatgtaa ggatatcatg
    54421 aaagacggct gtatcctcat tacacctgaa ttgtatggaa gtgatggatt cttcatcagt
    54481 caatttcgca agatatcgga ttaggaagga actgacacat ggaaatttca ttattaacag
    54541 atgttggtca gaaacgaaca aataaccaag actatgtcaa ccactatgtc aatagagctg
    54601 gacgtaccat gattatttta gctgatggga tgggaggtca tcgtgcaggg aatatcgcta
    54661 gtgaaatggc ggtcacagac ctgggtgtag cttgggttga tacccagatc gatacagtca
    54721 atgaagtgcg tgaatggttc gcccattacc tagaaattga aaatcaaaag attcaccagc
    54781 ttggtcagga tgaagcttac agaggcatgg gaactacttt ggaagtcctt gctattattg
    54841 ataatcaggc tatctatgct catattggtg attcgcgtat cggcttgatt cgtggagaag
    54901 aataccatca gttgacgagc gatcattcct tggttaatga attgctcaag gctggtcaat
    54961 tgacaccaga agaggcagaa gctcatccgc aaaaaaatat tatcacccag tctattgggc
    55021 aaaaagatga aattcagcct gattttggga cagttatcct tgagtcaggt gactatctct
    55081 tgctcaatag tgacggcttg accaacatga tttcaggcag tgagattcgt gatattgtaa
    55141 ccagtgatat tcctttagca gataaaacgg agacacttgt tcgttttgct aacaatgcag
    55201 gaggtttaga caacattacg gttgcccttg tttctatgaa cgaggaggat gcagaatgat
    55261 ccaaatcggc aagatttttg ccggacgcta tcggattgtc aaacagattg gtcgaggagg
    55321 tatggcggat gtctacctag ccaaagactt aatcttagat ggggaagaag tggcagtgaa
    55381 ggttctgagg accaactacc agacggaccc gatagctgta gctcgttttc agcgtgaagc
    55441 gagagctatg gcagatctag accatcctca tatcgttcgg ataacagata ttggtgagga
    55501 agacggtcaa cagtatcttg caatggagta tgttgctgga ctagacctca aacgctatat
    55561 caaggaacat tatcctcttt ctaatgaaga agcagtccgt atcatgggac aaattctctt
    55621 ggctatgcgc ttggcccata ctcgaggaat tgttcacagg gacttgaaac ctcaaaatat
    55681 ccttttgaca ccagatggga cggccaaggt cacagacttt gggattgctg tagcctttgc
    55741 agagacaagt ctgacccaga ctaactcgat gttgggctca gttcattact tgtcaccaga
    55801 gcaggcgcgt ggttcgaagg cgactgtgca gagtgatatc tatgccatgg ggattatttt
    55861 ctatgagatg ttgacaggcc atatccctta tgacggggat agcgcggtga ccattgccct
    55921 ccagcatttc cagaaacccc tgccgtccgt tattgcagaa aatccatctg tacctcaggc
    55981 tttagaaaat gttattatca aggcaactgc taaaaagttg accaatcgct accgctcggt
    56041 ttcagagatg tatgtggact tgtctagtag cttgtcctac aatcgtagaa atgaaagtaa
    56101 gttaatcttt gatgaaacga gcaaggcaga taccaagacc ttgccgaagg tttctcagag
    56161 taccttgaca tctattccta aggttcaagc gcagacagaa cacaaatcaa tcaaaaaccc
    56221 aagccaggct gtgacagagg aaacttacca accacaagca ccgaaaaaac atagatttaa
    56281 gatgcgttac ctgattttgt tggccagcct tgtattggtg gcagcttctc ttatttggat
    56341 actatccaga actcctgcaa ccattgccat tccagatgtg gcaggtcaga cagttgcaga
    56401 ggccaaggca acgctcaaaa aagccaattt tgagattggt gaggagaaga cagaggctag
    56461 tgaaaaggtg gaagaagggc ggattatccg tacagatcct ggcgctggaa ctggtcgaaa
    56521 agaaggaacg aaaatcaatc tggttgtctc atcaggcaaa caatccttcc aaattagtaa
    56581 ttatgtcggc cggaaatctt ctgatgttat cgcggaatta aaagagaaaa aagttccaga
    56641 taatttgatt aaaattgagg aagaagagtc gaatgagagt gaggctggaa cggtcctgaa
    56701 gcaaagtcta ccagaaggta cgacctatga cttgagcaag gcaactcaaa ttgttttgac
    56761 agtagctaaa aaagctacga cgattcaatt agggaactat attggacgga actctacaga
    56821 agtaatctca gaactcaagc agaagaaggt tcctgagaat ttgattaaga tagaggaaga
    56881 agagtccagc gaaagcgaac caggaacgat tatgaaacaa agtccaggtg ccggaacgac
    56941 ttatgatgtg agtaaaccta ctcaaattgt cttgacagta gctaaaaaag ttacaagtgt
    57001 tgccatgccg agttacattg gttccagctt ggagtttact aagaacaatt tgattcaaat
    57061 tgttgggatt aaggaagcta atatagaagt tgtagaagtg acgacagcgc ctgcaggtag
    57121 tgcagaaggc atggttgttg aacaaagtcc tagagcaggt gaaaaggtag acctcaataa
    57181 gactagagtc aagatttcaa tctacaaacc taaaacaact tcagctactc cttaaaagta
    57241 gatggataat caaaatgagt aatcaatctt tgtcatgatt tcatggcaaa gatttttttt
    57301 gagtccagat ttgtgataga atagagggag ttgataaaag gaggaaagca tggaagaatc
    57361 aaaagaatta aatgccgtca ttgatgtgat tatgctagcg gggactattc tccttaaaag
    57421 tggctcagaa atccatcgtg tagaagatac catgattcga atcgcgcatt cgcaggggat
    57481 tgtggattgc aatgtccttg ccatgcctgc cgctatcttt ttctctattg aaaataccaa
    57541 tatttcgcgc atgaagcgcg tgacctcctc ttcttataac atcgaaaaag tctgcgatgt
    57601 gaaccagatt tctcgtcagc tggttggggg gcagattgat ttagaaacag cctttaagca
    57661 attgacggcc ttgcaagctc aaccccttcc ctatactaag ttgcaggtaa ctctggctgc
    57721 gacctttagt gctcctttct tttcagttat gtttagcgga aatatctacg acgcacttgg
    57781 ggcaggagtg gcgaccttat ttggttttgc cttttccctc tatgtggaaa aatttatccg
    57841 aattcccttt gtgacagcct ttgctggagc ctttgtcttt gggataattg cccagttttg
    57901 ggctcgctac acaggttttc cttcaacggc agatttgatt atagctggtg cggtcatgcc
    57961 gtttgtacca ggtattgcct tgaccaatgc ggtccgtgat attatgacca accacataaa
    58021 ctctggtatg agtaagatgt ttgaatccct gctcattacc ctcgctttag gggcaggaac
    58081 ttctgtcgcc ttggtattgg tgaactaata tgacactaac aaccttttta ttacaagcag
    58141 tagcaagttt tcttgccatt atcacttttt taatcgtact caatgtgcaa cggtctatgc
    58201 tcttacctgg agggattttg ggcatgactg tctggctaat ctatctcttg ctcaaggaac
    58261 cgaccaatgt cattgtagct accttcattg cagccattat tggttcttgt gtcagccaga
    58321 ttttaagtat tctttataag acacctgctg tggtctttat cttggccatt ttggcaccgc
    58381 tggttccagg ttatctctcc taccgaacaa ctgccttttt tgtgacaggg gactataata
    58441 aagcactggc aagtgcgacc ttggttgtca tgttggcttt ggtaatctct attggaatgg
    58501 ctagcggaac agtgattctc agactgtatc attatataaa aacacatcga gtatcgtaga
    58561 ttttacagaa ataaaagaat tttctgaaaa atgagataaa taaattaaca acgctttcta
    58621 tatgtgcgag aatatcgaac ttatgaacaa attgcggctg attttggtat tcacgaaagc
    58681 aacttaatcc gtcggagcca atgggttgaa gtaactcttg ttcaaagtgg tgttacgatt
    58741 tcaagaactc ctctcagttc tgaggacacg gtaatgattg atgcgacgga agtaaaaatc
    58801 aatcgcccta aaaaaacaat tagcgaat
//
LOCUS       DAVBNO010000008        54535 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVBNO010000008 DAVBNO010000000
VERSION     DAVBNO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 54535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.008"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(798..2048)
                     /gene="ilvA"
                     /locus_tag="VQ846_000475"
     CDS             complement(798..2048)
                     /gene="ilvA"
                     /locus_tag="VQ846_000475"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET3075113.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(2098..2454)
                     /locus_tag="VQ846_000476"
     CDS             complement(2098..2454)
                     /locus_tag="VQ846_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET3075114.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(2692..2928)
                     /locus_tag="VQ846_000477"
     CDS             complement(2692..2928)
                     /locus_tag="VQ846_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075115.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERADATKSARYIAKWILRNKKH"
     gene            complement(3035..4057)
                     /gene="ilvC"
                     /locus_tag="VQ846_000478"
     CDS             complement(3035..4057)
                     /gene="ilvC"
                     /locus_tag="VQ846_000478"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET3075116.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(4123..4599)
                     /gene="ilvN"
                     /locus_tag="VQ846_000479"
     CDS             complement(4123..4599)
                     /gene="ilvN"
                     /locus_tag="VQ846_000479"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET3075117.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(4592..6292)
                     /locus_tag="VQ846_000480"
     CDS             complement(4592..6292)
                     /locus_tag="VQ846_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET3075118.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVKFHA
                     "
     gene            complement(6493..8160)
                     /locus_tag="VQ846_000481"
     CDS             complement(6493..8160)
                     /locus_tag="VQ846_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET3075119.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(8163..8528)
                     /locus_tag="VQ846_000482"
     CDS             complement(8163..8528)
                     /locus_tag="VQ846_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET3075120.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(8685..8873)
                     /locus_tag="VQ846_000483"
     CDS             complement(8685..8873)
                     /locus_tag="VQ846_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET3075121.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(8989..9474)
                     /locus_tag="VQ846_000484"
     CDS             complement(8989..9474)
                     /locus_tag="VQ846_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HET3075122.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNVQIDSSILKGYRLPFEGLSYAAFFRYFIPKYVSESRVL
                     YLDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLINNGMWRAENVTQDL
                     I"
     gene            complement(9743..11287)
                     /locus_tag="VQ846_000485"
     CDS             complement(9743..11287)
                     /locus_tag="VQ846_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET3075123.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIRDAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMESEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            11500..11802
                     /gene="gatC"
                     /locus_tag="VQ846_000486"
     CDS             11500..11802
                     /gene="gatC"
                     /locus_tag="VQ846_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET3075124.1"
                     /translation="MKITQEEVTHVANLSKLRFSEKETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            11802..13268
                     /gene="gatA"
                     /locus_tag="VQ846_000487"
     CDS             11802..13268
                     /gene="gatA"
                     /locus_tag="VQ846_000487"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET3075125.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            13268..14710
                     /gene="gatB"
                     /locus_tag="VQ846_000488"
     CDS             13268..14710
                     /gene="gatB"
                     /locus_tag="VQ846_000488"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET3075126.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVKLEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEIQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDANQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            14834..15877
                     /locus_tag="VQ846_000489"
     CDS             14834..15877
                     /locus_tag="VQ846_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HET3075127.1"
                     /translation="MATMKAARWHAAKDVRIQEIEVPEVLPHQVKVAVKFTGICGTDL
                     HEFLDGPIFIPTDEHVYSGQKAPVTLGHEFSGEIIEVGSDVTRVKVGDRVAVEPILAK
                     NNLVGDYNLDPNLNFVGLAADGGFAKYCVLDGDLVHVIPDSLSYEQAALTEPAAVAVY
                     AVRQSSLKAGDTAVVFGLGPIGLLIVEALRAAGASKIYAVELSPERQAKAEELGAIVV
                     RPEEGETAVEAIHRLTNGGVDVSYEVTGVPVVLGQALAAVHKAGECMVVSIWEREANI
                     NPNEFAIQEKTLKGIIAYRHIFPKVLELMEQGYFSAEKLVTKKIKLENIVEEGFIELT
                     QDKSQIKILVEPE"
     gene            complement(16013..16096)
                     /locus_tag="VQ846_000490"
                     /pseudo
     CDS             complement(16013..16096)
                     /locus_tag="VQ846_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            16255..16815
                     /gene="efp"
                     /locus_tag="VQ846_000491"
     CDS             16255..16815
                     /gene="efp"
                     /locus_tag="VQ846_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET3075128.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            16837..17226
                     /locus_tag="VQ846_000492"
     CDS             16837..17226
                     /locus_tag="VQ846_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET3075129.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            17219..17641
                     /gene="nusB"
                     /locus_tag="VQ846_000493"
     CDS             17219..17641
                     /gene="nusB"
                     /locus_tag="VQ846_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET3075130.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(17691..17927)
                     /locus_tag="VQ846_000494"
     CDS             complement(17691..17927)
                     /locus_tag="VQ846_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET3075131.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(17940..18413)
                     /locus_tag="VQ846_000495"
     CDS             complement(17940..18413)
                     /locus_tag="VQ846_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075132.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMGLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFYLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVLFSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(18431..18616)
                     /gene="briC"
                     /locus_tag="VQ846_000496"
     CDS             complement(18431..18616)
                     /gene="briC"
                     /locus_tag="VQ846_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET3075133.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(18775..19542)
                     /locus_tag="VQ846_000497"
     CDS             complement(18775..19542)
                     /locus_tag="VQ846_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET3075134.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(19539..20405)
                     /locus_tag="VQ846_000498"
     CDS             complement(19539..20405)
                     /locus_tag="VQ846_000498"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET3075135.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNVGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(20442..21809)
                     /gene="accC"
                     /locus_tag="VQ846_000499"
     CDS             complement(20442..21809)
                     /gene="accC"
                     /locus_tag="VQ846_000499"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET3075136.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(21821..22243)
                     /gene="fabZ"
                     /locus_tag="VQ846_000500"
     CDS             complement(21821..22243)
                     /gene="fabZ"
                     /locus_tag="VQ846_000500"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET3075137.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(22240..22725)
                     /locus_tag="VQ846_000501"
     CDS             complement(22240..22725)
                     /locus_tag="VQ846_000501"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET3075138.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPNPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(22728..23963)
                     /gene="fabF"
                     /locus_tag="VQ846_000502"
     CDS             complement(22728..23963)
                     /gene="fabF"
                     /locus_tag="VQ846_000502"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET3075139.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(23985..24716)
                     /gene="fabG"
                     /locus_tag="VQ846_000503"
     CDS             complement(23985..24716)
                     /gene="fabG"
                     /locus_tag="VQ846_000503"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET3075140.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(24750..25670)
                     /gene="fabD"
                     /locus_tag="VQ846_000504"
     CDS             complement(24750..25670)
                     /gene="fabD"
                     /locus_tag="VQ846_000504"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET3075141.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(25663..26637)
                     /gene="fabK"
                     /locus_tag="VQ846_000505"
     CDS             complement(25663..26637)
                     /gene="fabK"
                     /locus_tag="VQ846_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET3075142.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(26756..26980)
                     /locus_tag="VQ846_000506"
     CDS             complement(26756..26980)
                     /locus_tag="VQ846_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET3075143.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(27040..28014)
                     /locus_tag="VQ846_000507"
     CDS             complement(27040..28014)
                     /locus_tag="VQ846_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET3075144.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(28014..28448)
                     /locus_tag="VQ846_000508"
     CDS             complement(28014..28448)
                     /locus_tag="VQ846_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET3075145.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(28542..29327)
                     /locus_tag="VQ846_000509"
     CDS             complement(28542..29327)
                     /locus_tag="VQ846_000509"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET3075146.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            29652..31016
                     /locus_tag="VQ846_000510"
     CDS             29652..31016
                     /locus_tag="VQ846_000510"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET3075147.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            31019..31390
                     /locus_tag="VQ846_000511"
     CDS             31019..31390
                     /locus_tag="VQ846_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET3075148.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGCKISRHFEVFTDKGKFLFASKDSGAILKIARDKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            31604..32878
                     /gene="serS"
                     /locus_tag="VQ846_000512"
     CDS             31604..32878
                     /gene="serS"
                     /locus_tag="VQ846_000512"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET3075149.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLANYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(32903..33802)
                     /locus_tag="VQ846_000513"
     CDS             complement(32903..33802)
                     /locus_tag="VQ846_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET3075150.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPNWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(33912..34460)
                     /locus_tag="VQ846_000514"
     CDS             complement(33912..34460)
                     /locus_tag="VQ846_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET3075151.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(34678..36000)
                     /locus_tag="VQ846_000515"
     CDS             complement(34678..36000)
                     /locus_tag="VQ846_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET3075152.1"
                     /translation="MLELLKSIDDFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPVITALVLSVFVAIAVFGGLKSISKVSTTVVPFMTIIYI
                     LGTLIVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSQVVIAETKKYFDK"
     regulatory      complement(36091..36179)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(36343..38679)
                     /locus_tag="VQ846_000516"
     CDS             complement(36343..38679)
                     /locus_tag="VQ846_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET3075153.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(38783..39331)
                     /locus_tag="VQ846_000517"
     CDS             complement(38783..39331)
                     /locus_tag="VQ846_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET3075154.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPVTTSWLKQIWVTNLIG"
     gene            complement(39328..39630)
                     /gene="zapA"
                     /locus_tag="VQ846_000518"
     CDS             complement(39328..39630)
                     /gene="zapA"
                     /locus_tag="VQ846_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET3075155.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            39717..40589
                     /locus_tag="VQ846_000519"
     CDS             39717..40589
                     /locus_tag="VQ846_000519"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET3075156.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNSISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKNA"
     gene            40601..41215
                     /gene="lepB"
                     /locus_tag="VQ846_000520"
     CDS             40601..41215
                     /gene="lepB"
                     /locus_tag="VQ846_000520"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET3075157.1"
                     /translation="MNLFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRLWPITRIGTF"
     gene            41343..43709
                     /locus_tag="VQ846_000521"
     CDS             41343..43709
                     /locus_tag="VQ846_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET3075158.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGFSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGKQKSQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGVALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(43757..45040)
                     /locus_tag="VQ846_000522"
     CDS             complement(43757..45040)
                     /locus_tag="VQ846_000522"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET3075159.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(45201..45593)
                     /locus_tag="VQ846_000523"
     CDS             complement(45201..45593)
                     /locus_tag="VQ846_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET3075160.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNSFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNITEKLDKLVLEND"
     gene            complement(45892..46521)
                     /locus_tag="VQ846_000524"
     CDS             complement(45892..46521)
                     /locus_tag="VQ846_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET3075161.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(46566..46805)
                     /locus_tag="VQ846_000525"
     CDS             complement(46566..46805)
                     /locus_tag="VQ846_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075162.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(46955..47221)
                     /locus_tag="VQ846_000526"
                     /pseudo
     CDS             complement(46955..47221)
                     /locus_tag="VQ846_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(47246..47557)
                     /locus_tag="VQ846_000527"
     CDS             complement(47246..47557)
                     /locus_tag="VQ846_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET3075163.1"
                     /translation="MDWDFADTCWEKDPREYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHSELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(47482..47724)
                     /locus_tag="VQ846_000528"
     CDS             complement(47482..47724)
                     /locus_tag="VQ846_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET3075164.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIFQKRKKQRLSIGILQTLAGKRILENTNMWLLTI"
     gene            complement(47725..48357)
                     /locus_tag="VQ846_000529"
     CDS             complement(47725..48357)
                     /locus_tag="VQ846_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3075165.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(48371..49366)
                     /locus_tag="VQ846_000530"
     CDS             complement(48371..49366)
                     /locus_tag="VQ846_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET3075166.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(49363..50061)
                     /locus_tag="VQ846_000531"
     CDS             complement(49363..50061)
                     /locus_tag="VQ846_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET3075167.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(50138..51148)
                     /locus_tag="VQ846_000532"
     CDS             complement(50138..51148)
                     /locus_tag="VQ846_000532"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET3075168.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(51132..52139)
                     /locus_tag="VQ846_000533"
     CDS             complement(51132..52139)
                     /locus_tag="VQ846_000533"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET3075169.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTETLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(52126..53079)
                     /gene="mvaD"
                     /locus_tag="VQ846_000534"
     CDS             complement(52126..53079)
                     /gene="mvaD"
                     /locus_tag="VQ846_000534"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET3075170.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(53061..53939)
                     /gene="mvk"
                     /locus_tag="VQ846_000535"
     CDS             complement(53061..53939)
                     /gene="mvk"
                     /locus_tag="VQ846_000535"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET3075171.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(54061..54183)
                     /locus_tag="VQ846_000536"
     CDS             complement(54061..54183)
                     /locus_tag="VQ846_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein C"
                     /protein_id="HET3075172.1"
                     /translation="MTTGWFQVNGRWYYAYSSGALAVNTIVDGYSVNYNGEWVR"
BASE COUNT        15951 a        12463 c        10209 g        15912 t
ORIGIN      
        1 tatattcaac gaaaataatt agttcagaat tttcttactt cctttttaaa tccgataata
       61 tataggtttt gctagcctct acaccataaa tatttgctaa taaaaatact gtattattac
      121 cctcttaagg tgcgaaatta ttcaagttct taaaaaatgc ttctctattt ccgtaagata
      181 gcttatttct tcgatttaag aactgaagat gatagttcta acctttagaa atccaagcct
      241 tcactagctc aaaaactcct atctttcttc ctcaataaat catctgagag ataggtattc
      301 cttatgtcct taaaattcat cactcctttg gatattgagg tgctagccta gaagcagaca
      361 cccgattatc taaaccatcc ttaatagaga tgacaagaca gataagctca gttaattact
      421 tatatatctt tttaatatgg tatatttgtc aacaaaaaga aatcaaatca taccttatac
      481 tcaatgaaaa tcaaagagca aactaggaag ctagccgcaa gttgctcaaa acactgtttt
      541 gaggttgcag atagagctaa cgtggtttga agagattttc gaagagtatt aaagacagtg
      601 tgactatgct tgtcaataga taggaaatca aaatgaagga tggaaatctg agccaaacat
      661 cataagtgct ataatgaaca gaagtaagaa ttgcacgata agttttctaa aaccatagga
      721 atcgtcccct ttcttcatct tcattatagc acatatacat cagttgtaca aataatatga
      781 tattttttta ttagtcctca gacaagcata ttataaagcg tttcattacc atttaagtta
      841 atataagctg gatcaaaacc ttccattcta cgaatcaaac ctgcataatc atgcttatct
      901 gctaaagcga tcccaattaa tactgggcct gttcccttgc tagctcgttt gatatactca
      961 aaacgtgtga tatcatcatt tggccccagg atatcattta caaactcacg caaagctcct
     1021 ggacgttgtg ggaaattgac cacaaagtaa tgtttgatac catcataaat caaggcacgc
     1081 tcttccattt ctggcatacg gttgatatca ttatttcctc cagaaatgat acaacaaatg
     1141 gttttcccct taatatattc agctaaaacc tctaaagagg cgatactagc cgctccagca
     1201 ggttctgcga ctatcccttg cttagagtaa aggtcaatca aggtttcaga aatcaatccc
     1261 tcatcgacac ctaccaaagt tttaatatgt tgacgagttg cttcataggt caactgacct
     1321 accttttgca cagcaatccc atcagcaaat ttatcaattt ccttgagttt gactggacct
     1381 ccagcctcaa aggcagcttt catggaacgc gctccattcg cctctactcc gataacctca
     1441 atctctggac ttgtttcctt gatataggta gaaaccccgg caatgagacc gccaccacca
     1501 acaggaacca agacagcatc aaaatcaatc gattcttttc gagcttcttc taaaatctca
     1561 taagcaactg ttccttgacc tgcttgaaca tgagcatcat caaaaggatc aataaaggta
     1621 cgattttcag agactgtaaa ttcttgagct gctttggctg aggcatcaaa ggtatctcca
     1681 actagtttaa tagttacaaa atccccacca aaaaagcgaa cctgaccaat cttttgttgt
     1741 ggcgtagtaa tgggcataaa gatagtagca ggaattttca tttcattaca agtataggct
     1801 actccctgcg catgatttcc cgcagaagcg cagactaccc cacgttcacg ttcttccttg
     1861 ctgagctggg aaatggcata ataggcacca cgaattttaa aggagcgaac acgctgggca
     1921 ttttcttttt tcaaataaat cttagcacca tacttctccg ataaataatg atcgtaatcc
     1981 agtggagtat tcacaaccac accgttcaag accttgtgag ccttgattat atcttttgaa
     2041 cttaacatat aaaacctctt tttctataaa aaacagtcac taatgactgt tttttagtta
     2101 ttcatctccg aaaacttcag taacactttc atggtattta tgagccatac gaatcgaaaa
     2161 ctctttagga agttcataac gatttgtgga aatccaatca tataaccatt ctaccaaact
     2221 ttcttcacta tcattacgat ggaagataaa atagtttaaa atttctttat attctacatc
     2281 attcaaatcc aacgtgtcta caaattgact acgaggaaca aaattagaat ctaattttct
     2341 tagttgatct gtcaattctc ccaattgtaa atccaactga aaaaatagtt tttgcagttg
     2401 cctatcagtt tcatcacatc ctgtcttgat agaaaacaaa ttggtaatat tcatatgatt
     2461 tcaaccccta aataagtaca tctaatatcc cacgaatagc tgaagctaaa gttttcgcag
     2521 tccagtcatt taaacccgat tctttcaatc cagatgcaat aatttgctct atttgatata
     2581 aatcgccatc tatcgtctca tttattctgc gaagaggacc ttcaatatag gataaagctt
     2641 ctccgaatct aacagctgat ttaggtccta atgatttacc aactatattt gttaatgttt
     2701 tttatttctc aaaatccatt tcgctatata acgagcagat ttagtcgcat cagctctctc
     2761 aaacggagaa tgaatttcaa gaccttcttc tctagctatt tgtgccaatt tgaagaattg
     2821 ttcaaaggtc atatgcaacc taccatcttc aaaattagca tcatcataaa tgttaattac
     2881 agcattttca tcatacgacc aatctgcttt attttggtct gctagcacta ttaccgacgg
     2941 tgtaattaca ttatacaact agaaccaata ataacgatgc aatttttctc ataatctttt
     3001 tcataaaatc atctccagcg ctatatattt ctaattagtt atagattttg aatgcatcat
     3061 cgtcgttttt accaacgaat ggcattgctt tacgcaattc tgcaccaact ttttcaattt
     3121 caaggttagc tgcttgttca cggtaagcag tcaattttgg acgtccagct ttatagtcat
     3181 ttacaaagtc atttgcaaac ttaccatttt ggatgtctgc caagacagcc ttcatatttt
     3241 ctttaacttg ttcagtgatt acacgtggac ctgatacata gtcaccgtat tcagcagtgt
     3301 ttgaaataga ctgacgcatt ttcttgaatc caccttcgta gatcaagtca acgatcaatt
     3361 tcatttcgtg aagaacttca aagtaagcca attctggagc gtaacctgct tctgtcaaga
     3421 cttcgaaacc tgcttcgata agggcagtca aaccaccaca aagtacagct tgttcaccaa
     3481 acaaatcttc ttcagtttct tctttgtaag ttgtttcaag aagacctaca cgagccgctc
     3541 caacaccttt acaccagtcc atagcaatgt ttttagcatt tcctgttgca tcttggtata
     3601 ctgcataaag agctggaaca ccaaatcctt cttcgtaagt acgacgtacc aagtgtcctg
     3661 gtcctttagg agcacacatg aagacatcta catccgcagg aactttgata aattcaaagt
     3721 ggatgttgaa accatgggca aatccaactg cgtttccagc ttccaagttt ggagcgattt
     3781 ctgcttcgta caattcttgt tgaatttcgt ctggcgccaa gatcatgata acatcagcca
     3841 acttagtagc ttctgctact gtgtaagtat caaatccatc ttcttttgct ttatcaaaag
     3901 atttacctgg acgtacaccg ataataacgt cacgaccgga atcacgcaag ttttgagcat
     3961 gcgcatgccc ttgtgaacca taaccgataa cggcgatttt tttaccgtca agtgctgcta
     4021 ctttaacatc tttttcatat tccatttgaa ctgtcatagc ttttctctct tttctattat
     4081 ttattgcctt ttaggctggt ttaataaatt taagttggat ttttaatcgc gggtaaatcc
     4141 agttgcaccc gttcgagcaa tattgcgaat accgtatggg cgaatgactc gcaatagggc
     4201 ttcgctcttt tctgcatttc ccgtcatctg aatggtaatc gagcttggcg ctacgtctac
     4261 cactgttgca cggaaaggtt gaataatcgc taaaatctca gctctcttct cagctggcgc
     4321 tgacatctta accaaaatca cctcgcgctc caaatgaggc ttgtctgtaa tatctcgaat
     4381 gcgaatcaca tcaatctgac gattgagctg tttgatgatt tgctccactt catcatgaga
     4441 agcaacatca ataataatag tgatacgcga tacattcgga tcttctgttg ctccaacaga
     4501 gatgctttca atattaacct gacgacgaga taggacacct gtaaagcgat tgagtactcc
     4561 tgaacgattt tgtagttttg ctgttaacat tctacgcatg gaacttcacc cccaacatct
     4621 catgattact cttaccagcc ggtaccattg gtaacacctg ttccttacga gaaatatcta
     4681 cctcaattag cataggaaca tcctcagtga tgacttcaag gtcttgagcc aaggtctcag
     4741 gattgtcaaa cttatagttt ttaataccat aagcctgcgc catcaattgg aaatcaggaa
     4801 gggtatcaaa gaccgactct gatgttctgc cttcatagaa ggattcctgc cactggcgaa
     4861 ccattccaag tgaatgattg ttcagcataa ccaccttgat tggcaccttg taaatattca
     4921 aaatagccaa ctcctggttg gtcatttgga aaccaccatc cccaacaaac aagactactt
     4981 ccttatctgg gttagcaatt ttagcaccga ttgctgctgg aattccaaag cccattgttc
     5041 ccaaaccacc tgaagtcact aactgacgct cattttggta gggataatac tgagctgtcc
     5101 acatttggtg ttgaccaacg tctgttacca caatggcatc tccattcgtc aattcaccaa
     5161 ttcgttcaat aactgcttgc ggttgaacca cacgctcttt cttgtcataa gaacgaacac
     5221 gattcttgtc tttagtgact ttctcaatcc acttttcagt attgttgtga actgttggtt
     5281 ctgctagcaa catttgcaag gccttcttag catctccaac tacaggaatg tctgcactga
     5341 taatcttgcc aatctcagct gggtcaatat caatgtgggc aaccttagca ttcttagcga
     5401 aagtcttagg attccccgtc aaacggtcat cgaaacgaga accaatacta atcataaagt
     5461 ccgcttccgt catggcaata tttgctgcga atgacccgtg catgcctccc attccaagaa
     5521 agagtgggtg actcgttgca atcgttcctt gtcccaaaag actggttacc actggaattt
     5581 gatagcgttc tgcaaattca tttagttccg tagcagcctc agcataacta attccaccac
     5641 cagctaacaa gactggcttt ttagccttgg acaattgctt caagattttc ttgatttgca
     5701 tatcattcgg ctcaagagtc ggctgataac ttggtaaatt cacttctggt gaataaatga
     5761 agtctgtttc taaagcagat atgtcttttg gtaggtcaat tacaactggc cctggacggc
     5821 ctgtagttgc gatatggaca gcttccgtaa tgatacgcgg aatatcagct gtctcacgaa
     5881 cttggtaatt gtacttagtg attggcatgg taattcccac gatgtctgcc tcctgaaagg
     5941 catccttccc aatccctgct cgcgccacct gacctgtaaa gaccaaaagg ggaacgctat
     6001 cgctcatggc atccgcaatc cctgtaatgg catttgttgc tcctggtcca ctagtgacga
     6061 cggcaacacc caactttcca gttgatttgg cataaccttc agcttcatgc aaacaacctt
     6121 gctcatggcg ccctagaatg tggcgaatgc ctttaaaatt atatatcgca tcataaaaag
     6181 gcaaaaccgc accaccagga taaccaaaga tggtatcaac tcctaaatca cgaagtgttt
     6241 ccaaaactag gtccgacccc gtcttaggag attctaaact gattttctcc attattcccc
     6301 tttctttcct cttaaaaata acttgttact atcataccat tttttcaaaa tttttcaaga
     6361 taaaagaaga aattttctga attttctatt ttaacgttta tttatgaatg ttatttttgt
     6421 ttttattaaa aagataccga tttcatttac taaaaaagaa aaaagaactg atttctcagt
     6481 ccttcattaa tcttattcca cactaaatag gtatgggtaa acaggttgtt gaccttggtg
     6541 aatctcgact tcaacgtctt cgaattcttc tacgatttct tgagcgattt cattggcaag
     6601 ttcttcgctt ccgtcttcac cgacatagaa ggttacgatt tcactgtctt catccaacat
     6661 atgtttcaag gtttcagtca atgtttggtg catatcaggg tttgacacaa gaatttttcc
     6721 atccaccata cctagattat cgttttcatg gatttctaag ccatcgatcg ttgtatcacg
     6781 cacggctgtt gtgacgcttc cgctaacaac atcgctaaga gcagctgtca tacgctcttg
     6841 gttttcttca atggacttgc ttggatcaaa ggcaagaaga cttgtcatac cttgaggaag
     6901 agtgcgagcc tctaccacta ctgctggttg ctccaaaact tctgccgcag attgagctgc
     6961 catgaagatg ttcttgttgt ttggcaagaa gatgatgtta cgggcattga cctgttcaac
     7021 agccttgata aagtcttctg ttgaagggtt catggtttga ccgccttcga taacataatc
     7081 cacgccttga gaacagaaga tatctgctag acctttacca gccaccacag caatcaaagc
     7141 atactctttt tcttcagccg acttgataac ttgagtagct tctttctcaa cctgtgcttc
     7201 gtgttggtta cgcatattgt caacttttac cttgaccaag ctaccatatt tgagaccttc
     7261 ttgcataaca agtcctggat cttctgtatg aacatggact ttgacaattt catcatcgtt
     7321 aacaacgagg agagaatccc caagctcatc caagtagtta cggaattcat cgtagtcaaa
     7381 atctttagca taggttggac cttgcttaag agctaccatg atttcagtac agtaaccaaa
     7441 cgtgatgtcc tcagtcgcta cgtgaccagc tacagactta tgatgctcta cattgatcat
     7501 ctcactcatg ttggcaggag tcgctacaaa gtcctcagat gcaatatatt cgccagtaag
     7561 ggctgaaagg aaaccttcgt agatgaagac caatccttga ccacctgagt ccacaacgcc
     7621 aacttctttc aatactggaa gcatgtctgg tgttttagct agagctgttt tagcaccttc
     7681 caaggctgcg cgcatgactt caacagcgtc atctgtttgc tcagcttttt tcttagcacc
     7741 gatagcagct ccacgagaaa ctgttaaaat cgttccttca acaggtttca tcactgcctt
     7801 ataggcaact tccacacctg attggaaggc cagagccaag tcttgacctg ttaactcgtc
     7861 tttatccttg atagcttggg aaaatccacg gaaaagctga gacgtaatca ctcctgagtt
     7921 cccacgcgca cccatcaaaa gccctttggc aagaatgctc gctacttctc caactgtaga
     7981 agctggcttg tctgcaactt ctttagcacc attttcaatg gtcattccca tatttgtccc
     8041 agtatctcca tctgggactg gaaagacgtt taatgaattg acatattcag cttgcttatt
     8101 caagcgagtt gatgcagcct gcaccatttc ttgaaataag ctagtagtaa tttttgacac
     8161 ggttattctc ctacaacttt gatattttga atgtagacat ttacagtctg agcagtaatt
     8221 ccaagctggt tttccaagct aaaggcaaca cgctcttgaa tgttttttga cacttcacta
     8281 atctttgttc cgtagcttaa cacggtatat acatcaactg caatactgcc atcttcggct
     8341 gcctttacga cgacaccttt agaataattt tccttaccta gcagggcttg gaaattatct
     8401 ttgagggcat ttttactagc cataccgacc acaccaaaaa tctcagttgc tgctccacct
     8461 acgacggttg caatcacttc atctgttagt tcgatttgac catcttttgt attaattttt
     8521 acagtcatcc tttttacctc aactagttga tactctattt tatcatattt cagcccaagt
     8581 gtaaaagcag aatgatgtat cagcggatat ttactctatt tttcaaacga tgttataccc
     8641 acaataaaag aaaaaagacc ctaaggtctc ctttgctttt attattaaac gcgttcaact
     8701 ttacctgatt tcaaagcacg agctgaagcc caaacttttt taggtttacc atcgataaga
     8761 acagtaactt tttgaaggtt tggttttacg gcacgttttg tttggttcat cgcgtgtgaa
     8821 cggttgtttc ctgatacagt cttacgacct gtaaagtaac atactttagc cattgtgttt
     8881 tcctcctatt agatctaata tagcggatgt gctagcacca cataccgtac tatgttatca
     8941 cactttcttg ttttttgcaa gggaattgga agatttttta tttgtgtctt aaatcaggtc
     9001 ttgcgtgaca ttttctgctc tccacatgcc attgttgatt aacagaacac cagaattaaa
     9061 attatgtgta taaaaatcat ctctaactgc agctaagggt atagccgtca agtccaaatc
     9121 ccacagctca tctatcgatt ttcttacaac aatatctgaa tccaaataca gtacacgaga
     9181 ctcgcttaca tactttggaa taaaatacct aaaaaaagcc gcatatgaaa gtccctcaaa
     9241 ggggagacga taacctttca gaatactact gtcaatctga acattcacaa tctcactatt
     9301 caaagtctct agtctttttt ccatcaattg gaaccattct cgcggaaggt catcattaaa
     9361 aacataaaac ttaagattat aatgatgaac acaaagagat tttattgttg tttcaacttt
     9421 atccatataa gcattatctg cacctaagac aatcgctttt ttctcttctt tcactcttta
     9481 tcttatttct ttttattccc atcatattat tcccatcata tgtttctacg taaccattat
     9541 tttcgcctat tcgttcgtaa aaccatacca gtggagattt tagatgaagt cccattacgg
     9601 tttacatttt ttacattacg acacggagtt ttacaaatcg atttcatttg ccaaacgtag
     9661 ttagtgaggc agttagctag ttcgccaaat agcgactagc gtccaacaat ttggaacttt
     9721 agttccaatt gttggtactg agtcacatct tctcctctaa ctctacgtct ggatacttgt
     9781 ccgcaaacca gcggagggca aagtcatttt caaagagaaa gactggttgg tcaaaacggt
     9841 ctttggctaa gatattgcga cttgacgaca tccgttcatc caagtcctca ggcttgatcc
     9901 aacgaacggt ctttttaccc attgggttca taactacttc cgcattgtac tcgctttcca
     9961 tgcggtgttt aaagacttca aactggagtt gacctacagc gcctagcatg tactcacctg
    10021 tttggtaatt cttataaagc tgaacggctc cttcttgcac caattgctca atccccttgt
    10081 ggaaggattt ttgcttcata acattcttag cagaaacttt catgaaaatt tcaggagtaa
    10141 aggttggcag tggttcaaat tcaaacttgt tttttccaac cgtcaaggta tccccaacct
    10201 gataagtacc ggtatcgtaa accccgataa tatcacctgc cacggcattg gtcacattct
    10261 cacgactctc cgccataaac tgggtaacat tagatagttt ggcaccctta ccagtacgag
    10321 ggagattgac actcatgcca cgctcaaatt cgcctgatac gatacggaca aaggcaatac
    10381 ggtcacggtg acgaggatcc atgttggctt ggattttaaa gacaaagcct gagaaatcct
    10441 tgtcataagg atccacaatt tcaccgtctg ttttcttgtg accatgtggt tctggagcaa
    10501 acttgaggaa gatttcaagg aaggtctgca caccaaagtt tgtcagggct gaaccgaaaa
    10561 agacaggcgt caattctcca gccagaatag cttcctctga aaactcattc ccagcttcat
    10621 ttaaaagctc aatgtcatcc ttgacttgct cgtagaaagg attgctacca aaaagtttgt
    10681 ctccatcttc taggctagca aaacgctcat cccctttgta aagctctaaa cgttggttat
    10741 agaggtcata caagccctca aaggctttcc ccatcccgat aggccagttc atagggtagc
    10801 tagcaatgcc caagatttct tccaattctt gcaagagatc caaaggctca cgaccgtcac
    10861 gatccaactt gttcataaag gtaaagactg gaatgccacg atgtttcaca acctcaaaca
    10921 atttctttgt ttgagcctcg atccccttgg cagagtccac gaccatgacc gcagcatcca
    10981 ccgccatcaa ggtacgatag gtatcttctg agaagtcctc gtgccctggc gtgtctaaga
    11041 tattcacgcg cttgccgtcg tagtcaaatt gcataacaga tgaagtaaca gaaatcccac
    11101 gttgcttctc gatatccatc cagtcagatt tagcaaaagt ccctgttttc ttccctttta
    11161 ccgtaccagc atcacgaatc tcacccccaa agtagagtaa ctgctcagtg atggttgttt
    11221 tccccgcgtc cgggtgggag ataatggcaa aggtacgacg tttcttaatt tcttcttgaa
    11281 tattcataag ttctctttct ttgattctct atttttcttg tttcaatagc tgagaatgat
    11341 ttttacattg gattttacca ttcctttcga cactccatta tatcggattt tagcattttt
    11401 ttcaatttct atttcttttc acttccccct cccttattta taggaaaata tggtaaaata
    11461 gaacagacta aaaatcatca tttcacgaaa ggatgcaaga tgaaaattac gcaagaagag
    11521 gtaacacacg ttgccaatct ttcaaaatta agattctctg aaaaagaaac tgctgccttt
    11581 gcgaccacct tgtctaagat tgttgacatg gttgaattgc tgggcgaagt tgacacaact
    11641 ggtgtcgcac ctactacgac tatggctgac cgcaagactg tactccgccc tgatgtggcc
    11701 gaagaaggaa cagaccgtga tcgcttgttt aaaaacgtac ctgaacaaga caactactat
    11761 atcaaggtgc cagctatcct agacgatgga ggagatgcct aatgactttt aacaataaaa
    11821 ctattgaaga gttgcacaat ctccttgtct ctaaggaaat ttctgcaaca gaattgaccc
    11881 aagcaacact tgaaaatatc aagtctcgtg aggaagccct caattcattt gtcaccatcg
    11941 ctgaggagca agctcttgtt caagctaaag ccattgatga agctggaatt gatgctgaca
    12001 atgtcctttc aggaattcca cttgctgtta aggataacat ctctacagac ggtattctca
    12061 caactgctgc ctcaaaaatg ctctacaact atgagccaat ctttgatgcg acagctgttg
    12121 ccaatgcaaa aaccaaaggc atgattgtcg ttggaaagac caacatggac gaatttgcta
    12181 tgggtggttc aggtgaaact tcacactacg gagcaactaa aaacgcttgg gaccacagca
    12241 aggttcctgg tgggtcatca agtggttctg ccgcagctgt agcctcagga caagttcgct
    12301 tgtcacttgg ttctgatact ggtggttcca tccgccaacc tgctgccttc aacggaatcg
    12361 ttggtctcaa accaacctac ggaacagttt cacgtttcgg tctcattgcc tttggtagct
    12421 cattagacca gattggacct tttgctccta ctgttaagga aaatgccctc ttgctcaacg
    12481 ctattgccag cgaagatgct aaagactcta cttctgctcc tgtccgcatc gccgacttta
    12541 cttcaaaaat cggccaagac atcaagggta tgaaaatcgc tttgcctaag gaatacctcg
    12601 gtgaaggaat taacccagag gttaaggaaa ccattctaaa tgccgctaaa cactttgaaa
    12661 aattgggtgc tattgtcgaa gaagtcagcc ttcctcactc taaatacgga gttgccgtat
    12721 actacatcat cgcttcatca gaagcttcat caaacttgca acgcttcgac ggtatccgtt
    12781 acggctatcg cgcagaagat gcaaccaacc ttgatgaaat ctatgtaaac agccgaagcc
    12841 aaggttttgg tgaagaagtg aagcgccgta tcatgctggg tactttcagt ctttcatcag
    12901 gttactacga tgcctactat aagaaggctg gacaggtccg tacacttatc attcaagatt
    12961 tcgaaaaagt cttcgcggat tacgatttga ttttgggtcc aactgctcca agtgttgcct
    13021 atgacttgga ttctctcaac catgacccag ttgccatgta cttagccgac ctattgacca
    13081 tacctgtaaa cttggcagga ctgcctggaa tttcgattcc tgctggattc tctcaaggtc
    13141 tacctgtcgg actccaattg attggtccca agtactctga ggaaaccatt taccaagctg
    13201 ctgctgcttt tgaagcaaca acagactacc acaaacaaca acccgtgatt tttggaggtg
    13261 acaactaatg aactttgaaa cagtcatcgg acttgaagtc cacgtagagc tcaacaccaa
    13321 ttcaaaaatc ttctcaccta cttctgccca ctttggaaat gaccaaaatg ccaacactaa
    13381 cgtgattgac tggtctttcc caggagttct accagttctc aataaagggg ttgttgatgc
    13441 cggtatcaag gctgctcttg ccctcaacat ggacatccac aaaaagatgc actttgaccg
    13501 caagaactac ttctatcctg ataaccccaa agcctaccaa atttctcaat ttgatgaacc
    13561 aatcggatat aatggctgga ttgaagtcaa actagaagac ggtacgacca agaaaatcgg
    13621 tatcgaacgt gcccacctag aggaagacgc tggtaaaaac acccatggta cagatggcta
    13681 ctcttatgtt gacctcaacc gccaaggggt tcccttgatt gagattgtat ctgaggcaga
    13741 tatgcgttct cctgaagaag cctatgctta tctgacagcc ctcaaggaag ttatccagta
    13801 cgctggcatt tctgacgtta agatggagga aggttcgatg cgtgtggatg ccaacatctc
    13861 ccttcgtcct tatggtcaag agaaattcgg taccaagact gaattgaaga acctcaactc
    13921 cttctcaaac gttcgcaagg ggcttgaata cgaaatccaa cgtcaagctg aaatacttcg
    13981 ctcaggtggt caaattcgcc aagaaacacg ccgttacgat gaagctaaca aaacaaccat
    14041 cctcatgcgt gtcaaggaag gggctgcaga ctaccgctac ttcccagaac cagacctacc
    14101 cctctttgaa atttctgacg agtggattga ggaaatgcgg actgagttgc cagagtttcc
    14161 aaaagaacgt cgtgcgcgtt atgtatctga ccttggttta tcagactacg atgctaatca
    14221 gttgactgct aataaagtca cttctgactt ctttgaaaaa gctgttgccc taggtggtga
    14281 tgccaaacaa gtctctaact ggctccaagg ggaagtcgct cagttcttga atgctgaagg
    14341 taaaacactg gaacaaatcg aattgacacc agaaaacttg gttgaaatga ttaccatcat
    14401 cgaagacggt actatttcat ctaagattgc caagaaagtc tttgtccatc tagctaaaaa
    14461 tggcggtggc gcgcgtgaat acgtggaaaa agcaggtatg gttcaaattt cagatccagc
    14521 tatcttgatc ccaatcatcc accaagtctt tgccgataac gaagctgctg ttgccgactt
    14581 caagtcaggc aaacgtaacg ccgacaaggc ctttacagga ttccttatga aggcaaccaa
    14641 aggccaagcc aacccacaag ttgcccttaa actacttgca caggaattgg cgaagttgaa
    14701 agaaaactag acagaacaaa accagcccta aggttggttt tttcttgaca aatagaaaga
    14761 aaacgtttac aatattgcgt gtaaagtttc caaacaagaa aactaaaaat cgcaacaata
    14821 aggagttatt tatatggcta caatgaaggc agctcgctgg catgcagcga aagatgttcg
    14881 tattcaagaa atagaagttc ctgaagtact tccgcaccaa gtaaaagttg cagttaagtt
    14941 cacaggaatt tgtggtactg acctccatga atttttagat ggacctatct ttatcccaac
    15001 agatgagcac gtctattctg gtcaaaaagc accggtaaca ctgggacatg aattttctgg
    15061 tgaaatcata gaagtcggaa gcgatgttac ccgtgttaaa gttggtgatc gtgttgctgt
    15121 ggaaccaatt ctagctaaaa ataacttagt tggtgattat aatttggatc caaaccttaa
    15181 ttttgtcggt ttggctgcag acggtggatt tgccaaatat tgtgttttag acggtgactt
    15241 agtacatgtc attccagata gcttgagcta tgaacaagct gctcttactg aacccgctgc
    15301 tgttgcagtc tatgctgttc gtcaatcttc tttgaaggct ggcgatacag ctgttgtctt
    15361 tggtttaggc ccgattggtc tattaattgt agaagctctt cgtgcagctg gtgcttctaa
    15421 gatttatgct gttgaactat cacctgaacg tcaagcaaaa gctgaagagt taggagctat
    15481 cgttgtccgt ccggaagaag gtgaaacagc tgttgaagct attcatcgtt taacaaacgg
    15541 tggtgtagat gtttcttatg aagtaacggg tgtgccagta gttttaggac aagccttagc
    15601 tgccgtacat aaagcaggtg agtgcatggt ggtctctatc tgggaacgag aagcaaatat
    15661 taatccgaat gaatttgcaa tccaagaaaa gacactaaaa ggaatcatcg cttatcgtca
    15721 tatcttccct aaagtattag aattgatgga acaaggctac ttctctgctg aaaaattagt
    15781 tactaagaaa attaaattgg aaaatatcgt cgaagaaggc tttatcgaat taactcaaga
    15841 taagtcacaa attaaaattt tggtagaacc agaataaaaa atcaatcttt ttaagacaat
    15901 actaacaggt cggagtaaac ttacttcggc cttttgaaat ataatactct tcgaaaatct
    15961 cttcaaacca cgtcagcgtc acattaccgt atatatggtt actgacttcg tcagttctat
    16021 ccacaacctc aaaacagtgt tttgagcagc ctgcggctag cttcctagtt tgctctttga
    16081 tttccattga gtatgagaag aaatatagtg gatactcttt aacattaacc actccacctg
    16141 actagccaat attctttttc gctcctacca actcccaata actattttgg ctttatttcc
    16201 agagtatttt atggtaaaat gaagagtaat aatatttgtt aaagaggtaa aaacatgatt
    16261 gaagcaagta aattaaaagc tggtatgacc tttgaaacag ctgacggcaa attgattcgc
    16321 gttttggaag ctagtcacca caaaccaggt aaaggaaaca cgatcatgcg tatgaaattg
    16381 cgtgatgtcc gtactggttc tacatttgac acaagctacc gtccagagga aaaatttgaa
    16441 caagctatta tcgagactgt cccagctcaa tacttgtaca aaatggatga cacagcatac
    16501 ttcatgaata cagaaactta cgaccagtac gaaatccctg tagtcaatgt tgaaaacgaa
    16561 ttgctttaca tccttgaaaa ctctgatgtg aaaatccaat tctacggaac tgaagtgatc
    16621 ggtgtcaccg ttcctactac tgttgagttg acagttgctg aaactcaacc atctatcaaa
    16681 ggtgctactg ttacaggttc tggtaaacca gcaacgatgg aaactggact tgtcgtaaac
    16741 gttccagact tcatcgaagc aggacaaaaa ctcgttatca acactgcaga aggaacttac
    16801 gtttctcgtg cctaatctct agaaagaggt cattctatgg gaattgaaga acaacttggc
    16861 gaaatcgtta tcgccccacg tgtacttgaa aaaatcattg ctatcgctac tgcaaaggta
    16921 gagggtgttc actctttttc aaacagatca gtgtctgata ccctttcaaa actttcactc
    16981 ggccgtggca tttatcttaa aaacgtggac gaagaactca cagcagatat ctatctctac
    17041 cttgagtacg gagtaaaagt tcctaaggta gcggttgcta tccagaaagc tgtcaaagat
    17101 gccgtccgta atatggctga tgtagaactc gctgctatca atattcacgt tgcaggtatc
    17161 gtcccagata aaacaccaaa accagaattg aaagatctat ttgacgagga cttcctcaat
    17221 gactagtcca ctattagaat ctagacgcca actccgtaaa tgcgcttttc aagctctcat
    17281 gagccttgag ttcggtacgg atgtcgaaac tgcttgtcgt ttcgcctata ctcatgatcg
    17341 tgaagatacg gatgtacaac ttccagcctt tttgatagac ctcgtttctg gtgttcaagc
    17401 taaaaaggaa gaactagata agcaaatcac tcagcattta aaagcaggtt ggaccattga
    17461 acgcttaacg ctcgtggaga gaaacctcct tcgcttggga gtctttgaaa tcacttcatt
    17521 tgacactcct cagctggttg ctgttaatga agctatcgag cttgcaaagg acttctccga
    17581 tcaaaaatct gcccgtttta tcaatggact gctcagccag tttgtaacag aagaacaata
    17641 aaaagaaaaa gtgtttgata gatatcaaac acttttttct ttgcctccca ctatctaaaa
    17701 aaatgataat agatataatt gtaaacaaaa atccagatag gttttgcatg attgagaaag
    17761 ttaaaaaaac tatggcagag aatcgttaat ctcagattgt cggtagaacg ataaacaagg
    17821 gcaaaaaaga aaccaatcag actataatat aataaactaa ttggatctct gtgagatagt
    17881 atcaaatggc taatcccaaa gatgatagca gataggataa catccaaata gtacttggac
    17941 tagggaaaga aggtattcat aaaataccct ctatcaagag tctcctcaaa aacaggaccg
    18001 atgattacag gcaggacaaa agataagata gtcgataaaa aggttggttg tccatttgaa
    18061 aaaagcacgg taaaatactc atcatgaata ttcctatgat taatcaaatg agcatagcgt
    18121 gcccaaaaat taccgagaat ctgataaacc acataagttg caaataagta gaagacaaat
    18181 gaccagttcc agctcttttt ctcaaagata aagagcatct ttttcttttt taacctccaa
    18241 attaatagaa ggaaacttcc cactaatccc attgttaaaa taagagaata gacatcagct
    18301 cctaacccta aaatgatcgt cacatacaat ccaattgttt gtggtaaata ggtagatagt
    18361 aaaataataa gcaaaaatat tccaaattgt cttagttttt ttgtgtttct catcgtactt
    18421 ttttgaaaga ttaccctgct cggaagccgt acttccaagc atctatataa gaattaagtg
    18481 ccccttgcct catataggga gcaaattctc tataatataa ccatctacta tatccatctt
    18541 cccaaacagc aagaccacct gaagtttgct ccaagtcctc agttgaaaga actgtaaatg
    18601 tatttgtacc tgtcattgca agtaccttct taaaatagat tgttgtaggc tcacatttat
    18661 agtatatttc tttttttgtc tattttatag cccatctcct caactggcaa tttttcgacc
    18721 tgaattacat ttttccataa aaaatgagac ctttctagtc tcatttagtc attcttagta
    18781 ttttctaaat cgttgatagc gttcttccag caactcttct agcggttttt gtgaaagtct
    18841 agccagctct gtttggagtt cttttttgac actcttaatc agttctttac tagaaagtcc
    18901 tatttcagaa atcaccttat ctaccacgtc catttctaac agttcatgcg aagtgatttt
    18961 catcagttct gctgcttcca tagcgcgagt accgtccttc cataaaatgg aagcaaagcc
    19021 ttctggactg agaatggcat agatagaatt ttccagcatc cagacacggt ccgcgacagc
    19081 tagagccaga gccccgcctg aaccaccttc accgataata atggcgataa taggaacttt
    19141 caggtcactc atttccatga gattgcgagc gatagcttcc ccttgaccac gttcttccgc
    19201 tccgacacca ggataagcac ctgctgtatt gataaaggtc acaactggac ggccaaattt
    19261 ctcagcctgt ttcatcaacc gcagtgcctt tcggtagcct tctggatgtg gttggccaaa
    19321 attccgtttg aggttgtctt gcaaactctt gcctttttgg ataccaacca ctgttacagc
    19381 ttgatctcca agccaaccaa taccaccaac aactgcacca tcatcacgaa aagaacggtc
    19441 accatgtaat tggataaatt catcaaaaat gcctgtcgca aagtccaagg ttgtcaagcg
    19501 actctgctca cgcgcttctc tgactatttt tgcaatattc atctaggact ccctccatgc
    19561 aatctgacta ggctagcaat cgtatctggt aagtctcttc ttttgacaat agcatccaca
    19621 aagccatgtt ctaataggaa ttctgccttt tggaaatcct caggcaagct ttcacgaacc
    19681 gtattttcaa tcacacgacg cccagcaaaa ccaaccaagc tctgtggttc agccagaatg
    19741 atatcgcctt ccatagcgaa agaagctgtc acaccaccag tcgttggatc tgtcaaaatg
    19801 gtcaggtaaa agagaccaac atttgaatgg cgtttaaccg ccgcagagat cttagccatc
    19861 tgcatgagac tcatgattcc ttcctgcata cgggctccac cagaggctgt gaataggaca
    19921 actggcaatt tttcgacagt cgcatactca aacaaacgag tgattttttc acctacaacc
    19981 gtacccatag aagccatgat aaagttagaa tccataatcc caagagccac agtctgacct
    20041 ttaataagag cagttcctgt cacaacggct tcatgcagac ctgttttttc acgcatagat
    20101 gccagtttct tttggtaacc agggaaatgc aagggatcct tgctttcaat ccctgtaaac
    20161 aattctttga aggttcccat atcaatcgtc aaagccaagc gttcttgggc agaaatacga
    20221 aaggtatagc tacagtgcgg acagatacgt tcacttccca gatccttctg atagatggta
    20281 tgcttacagc ctggacactg ggaaaataat tcatctggaa cctctggctt agcttgaggt
    20341 ttttccctaa ccgaacgatt gggattgatt cgaatatact tatctttttt actaaataga
    20401 gccattgatt ccccttttcg gtttaaactc ttaaagtcat tttattcttt ttcttgatat
    20461 ttaggtaaga aggtttccat caagaaggaa gtatcataat ccccagcaat gacattgcga
    20521 tctgaaatga ggtcaagctg gaaatctgca ttggtctgca ctccttcaat ctctaattca
    20581 tagagggcac gttgcatttt catcaaggcg tcaaaacgat tttcgccgtg tactatgatt
    20641 ttggcaatca tactatcata ataaggcgga atggtataac ctggataaac tgctgaatcc
    20701 acgcgcaaac caactccacc acttggcaga tagagattag taatcttacc tggacttgga
    20761 gcaaagttaa aggctgggtt ttctgcattg atacgacact cgatggcatg accacgtagg
    20821 acaatatctt cttgcttaac agacaaaggc tgacctgccg caatgcaaat ctgttcctta
    20881 acgatatcaa cacctgaaac aaactctgtt actggatgtt ctacctgaac acgagtattc
    20941 atctccatga aatagaaatt gctacttgct tcatcaagaa gaaattcaat ggttcctgca
    21001 ttctcatagc caacaaactc tgccgctcga acagcagcag cacctatttc atgacgcagc
    21061 gtttttccga ttgcaatcga gggactttct tccaaaacct tttggttatt cctttgaaga
    21121 gaacaatccc gttcacccaa gtgaatcaca tgtccatgct catcacctag gatttgaacc
    21181 tcaatgtgcc gagctggata gataacccgt tctatgtaca tggcaccatt gccataattg
    21241 gccttggcct cactagaggc agtttcaaag gcagaaacga ggtcatctgg tttttcaacc
    21301 ttacgaatcc ctttaccacc tccacctgct gaagccttga gcataacagg atagccaatt
    21361 ttttcagcaa caatcaaagc ttcttcagag ttatgcactt ctccatctga acctggtata
    21421 acaggcacac ctgctttaat catctgagca cgcgcattga tcttatcccc catcatatcc
    21481 ataacatgac cagatggacc gataaacttg atacctactt cttcacacat ggtcgcaaat
    21541 ttggaatttt cactgagaaa tccaaaacca gggtgaatag cttctgcctc agtcaagact
    21601 gcagctgata gaactgcatt aatattgaga taagactctg ttgccttgcc aggaccaata
    21661 caaactgctt catctgccaa aagcgtatga agagcttcct tatcagcagt tgaataaacc
    21721 gctaccgtcg caatccccaa ttcacgtgcc gcacggataa tacgaaccgc aatttcacca
    21781 cgattggcaa ttaaaatttt tcgaaacatg gagaacctcc ttagttccca attgcaaaag
    21841 taaggatacc actggctgca agcttgccat ccacttcagc ctttgcttca accacagcta
    21901 tggtgccacg acgttttaca aaagtcgctg tcataaccaa ttggtcgcct ggtacaactt
    21961 gcttcttgaa cttaaccttg tccataccag cgtaaaagac cagttttcct ttattttcag
    22021 gttttgataa ctccaacaca ccagcagttt gcgccaaggc ttccataatc agaacacctg
    22081 gcataactgg gtattgagga aagtggccgt taaagaaagg ttcgttaatg gtcacatttt
    22141 tgatagcaac aatggtatcc tcgctcactt ccaagacacg gtccactaga agcataggat
    22201 aacggtgggg aagagcttct ttgattcctt gaatatcgat catttgatac gtaccaatcc
    22261 tttaccaaac tcaaccattt cttcgttaga gacgagaatt tccgttacca caccatcctt
    22321 aggagctggg atttcattca tgactttcat ggcttcgata attaccaatg tttgaccttt
    22381 tttgacacta tcaccaactg taacgaaggc aggtttatct ggtccagcag ccaagtaaac
    22441 cactccaaca agtggactct ctacaagatt tccctcacta gccacacttg cttcagctgg
    22501 agctggaact tcttctgcta cagtctctgc tggagcagat gtaggagcta ctggattcgg
    22561 tgttgctaga acgggtgctg gagcgacttg agttgcaact tcaggcacag gtctcgcttc
    22621 attcttgcta aactgcaact catccgtccc atttttataa gaaaattctc tcaaacttga
    22681 ctggtcaaat tgagtcatca agtctttaat atcgtttaaa ttcatactta tctattctcc
    22741 caacgtttga aagcaagaac tgcattgtgg cctccaaaac caaaagtatt tgaaatagcg
    22801 tatggaattt ctttctccaa gccttgtcca taaacgacat tagcttcgat ataatctgat
    22861 acttcacttg tcccagctgt cattggtaca aagttatgac gcatagcttc gatggtgacg
    22921 atagcttcta ctgcacccgc agcccccagc aaatgtcctg taaaagactt ggttgatgat
    22981 acaggtactt ccttaccaag aacagctacg atagcaccac tttctccttt ttcattggca
    23041 ggagttgacg ttccgtgagc attgacatag gctacttgct ctggagaaat ctcagcttct
    23101 tccaaggcta gtttgatggc cttgatagct ccctgacctt ctggatgtgg agaagtcatg
    23161 tggtaggcat cacaagtatt tccgtaacca accacttcag ccaggatagt agctccacgt
    23221 ttttcagcgt gttcaagact ttctagaacc aacatccctg aaccttcacc cataacaaac
    23281 ccattgcgat ccttatcaaa tgggatcgaa gcacgagttg gatcctctgt agtagagaga
    23341 gctgttaagg cttggaaacc agcgatggca aaaggtgtga tagaagcttc tgttcctccc
    23401 accaacatca catcttggaa accaaactta atggagcgga aggcatcccc aatcgcatca
    23461 tttgatgaag agcaggcagt attgatagat ttacaaacac cgtttgcacc aaaacgcatg
    23521 gctacattcc cagaagccat atttggtaaa gcttttggaa gagtcattgg tttgacacgt
    23581 ttgggtcctt tttcatgaag gcgaagtacc tgatcttcaa tttccttgat tccaccaata
    23641 ccagatgcaa cgataacacc aaaacgatcc ctattaagag cctctacatc aagattggca
    23701 tgatttacag cctcttgggc tgcatacaag gcatataaag aatagttatc aaaacggttg
    23761 gtatcttttt ttacaaagta tttatcgaac ggaaaatctt ggatttctgc cgcattatgc
    23821 acatcaaagt cactatgatc aaattttgta atgccaccaa tgccgatttt cccagttgct
    23881 aaactattcc aaaattcttc tggtgtattt ccgattggag atgttactcc ataacctgtt
    23941 accactactc gatttagttt cattcttttc acctctagct ttcgctacat acttaagcca
    24001 ccatcaatgg caaccacttg accagttaga taatcttggc ctgctaaaaa tactgtcaaa
    24061 tctgcaacct gctctgcctg cccaaattct ttcatcggaa tctgagctag tgtagcttcc
    24121 ttaatcttat ctgataagat agctgtcata tcagactcaa tcattcctgg agcaatcaca
    24181 ttgactcgta tattccgact agcgacctcg cgtgccacag acttggtaaa gccaatcaag
    24241 ccagccttag aagcagcata gttagcttga ccaatattcc ccatcaaacc aacaacacta
    24301 gacatattaa tgatagcacc ttctctggct ttcatcatcg gtttcaagac tgattgtgtc
    24361 atattaaagg caccagtcag attgaccttg agcacttttt caaaatctgc ttctgtcatc
    24421 ttgagcataa gagtatcttg ggtaatccct gcattgttga ccaaaacatc tactgaaccc
    24481 agttctgcaa tagcttgatc aatcatacgc ttagcgtctg caaaatctga tacatctcct
    24541 gaaatgggaa ccaccttgat accatagttt gaaaactcag cgagcaattc ttctgagatt
    24601 gccccacgac tgtttaagac aatgttggct cctgcttgag caaacttgtg ggcgatggca
    24661 agaccaattc cacgactcga acctgtaata aagatatttt tatgttctag tttcattttt
    24721 ttcctttcaa aacttctact tattttagtc tatttttcta aaagtgctac taaactcgct
    24781 tgatcttcca catgagctaa gtgagcagtt tgatcaattt ttttaacaaa acctgacaag
    24841 actttccccg gtccaatctc gataaagttg cttatgcctg cttcttgcat gaccccaata
    24901 ctttcataga aacgaacggg ttccttgacc tgacgcgtca agagctgagc aatgtcctct
    24961 ttttgcatca cagcagcttc tgtattgccg actaggggac aagtaaaatc tgaaaaactt
    25021 acctgagcta gagtttcagc tagtttctgg ctagcaggct caaggagagc ggtgtgaaag
    25081 ggacctgaca ccttaagagg aatcaagcgt ttggcacctg cttcttgcaa aagttcaacc
    25141 gctcgatcaa ctgcaaccac ttctccagca atgacgattt gtgcaggtgt gttatagttg
    25201 gctggagtaa ccactccaag ttcagaagct ttttgacagg cttcttcaat gacctctact
    25261 ggcgtattga gaactgctac catcttgcca gagtcagcag gagccgcttc ttccatatag
    25321 gctccacgct tagctaccaa ggcaaccgca tcttcaaaat ccaaggcgcc gcttgccacc
    25381 aaggcagagt attctccaag agacaaacca gcaaccatat caggctgata gcccttttct
    25441 tgcaataaac ggtagatagc aaccgaagtc gctagaatgg ctggttgcgt atagcgggtc
    25501 tgattgagtt tgtcttcttc cgtatcgatg agataacgta aatcataacc gagcacctga
    25561 ctcgctcgat caatcgtttc tttgacaatc ggatactgat catagaaatc ccgtcccatc
    25621 cctagatact gggcaccttg accagcaaat aaaaaggctg ttttagtcat ttcttacaac
    25681 tcctgcccag cgagaggctt cttcttgaat tttcttagcg gctccgtaat acaaatcttt
    25741 taggatttct tcagctgttt cttctttaga aacaagccct gcgatttgac ctgccatgac
    25801 agagccacca tccacatcac cgtgaacaac tgctttggct agggcacctg ctcccatttg
    25861 ttcaaagatt tctaaatcag gatcttcctg cttaaaggca tctttttcag ccagttcaaa
    25921 atctctagtc aactgatttt taatagcacg aacagcatga ccaaagtgct gagctgaaat
    25981 cgtagtatca atatcccttg cttttaaaat tttctccttg tagtttggat gggcattcga
    26041 ctcttttgca actacaaacc gtgtccccac ctgtacagcc tctgcaccta gcataaagcc
    26101 agccgcagca ccttcaccat ccgcaattcc tcctgcagca ataacaggaa tagatatagc
    26161 tgtggctacc tgtcgcacca aggtcatggt tgttaattta ccgatatgcc ccccagcttc
    26221 cattccttct gcaataacag cgtctgcacc gattttttcc atgcgtttag ctaaagcgac
    26281 actaggaaca acaggaataa cgattatccc agcttcatgg aaacgttcca tatacttgct
    26341 tggatttcct gctcctgttg tgacaacttt aacaccttct tcaataacga gatccacgat
    26401 atcttccaca aagggagata agagcatgat gttgacccca aagggtttat cagtcaatga
    26461 tttgatttta tcaatattgg ccttgacaac ttctttcggg gcatttcccc caccgataat
    26521 tcctaatcct ccagccttgg aaacagcccc tgccaaatca ccatcagcaa cccaggccat
    26581 ccctccttgg aaaataggat agtcaatctt caataattct gtaatacgcg ttttcatagt
    26641 gcctccaacc ttccttgctt acgtaatagt tcgatttcac cataatttga cagtcaaact
    26701 attacctaaa caagagggag tgggtttctc cctactcctt ctactaatat tctgcttatt
    26761 ttgcttgctc ttcaacgtaa gcaaccaagt caccaactgt tttcaagtca ttttctgctt
    26821 cgatttggat atcaaaagca tcttcgattt ctgagattac ttggaacaag tccaatgaat
    26881 ctgcgtccaa atcatcaaaa gttgattcaa gtgttacttc tgatgcgtct tttccaagtt
    26941 cttcaacgat aatttcttgt actttttcaa atactgccat gataggactc ctttaaaata
    27001 aatagttttt ttataacaat gtgttcacca catgattacc taaattgtaa gaatgagcgt
    27061 gccccaggtc aagcctccac cgaagcctga tagaagaaca gtctggctac catctaaagg
    27121 gatgagacct tgttctacac actctgaaag taaaatcggg atactggctg cactggtatt
    27181 gccatattcc atcatattgg ctggaagttt ggctcggtca acaccaattt ttctagccat
    27241 cttatccaaa atacggtcat tggcttgatg aagtagcaga taatccaagt ctgtcacctc
    27301 tataggagat tcatcaatag tctgcttgat agacttggct acatctcgaa tggcaaaatc
    27361 aaagactgtg cgtccatcca tcttcaaaaa cgaatctgca ctttcttgat ctgaaaatgg
    27421 agaatgcaaa cctgaatgcc cataagttaa acactcgctg cgacttccat cgctattgag
    27481 actctcagct aagaaatgct cttgctcgct agcttctaac aagacaccac cagcaccatc
    27541 tccaaacaac acagctgttg atcgatccga ccaatcgact gccttagaga gggtttcact
    27601 accaatcacc aagccttttt gaaagcgacc agaagcgata aacttttcag cagttgaaag
    27661 agcaaataca aatccactgc aagccgcggt taagtcaaaa gcaaaggctt tattagcgcc
    27721 aatattagct tgaacacgag cagctgtaga gggcatcatc gaatctggag taatggtagc
    27781 taggatgata aaatccagtt cttttcctgt tattccagct tttgccatca gtttcttagc
    27841 aacctctgta gccaaatcac tggtagattc tgttcttgaa atatgccttt gtcgtattcc
    27901 cgttcgactt gaaatccact catcattggt atccataatc tgagccaagt cgtgatttgt
    27961 aaccacttgc tctggcacat aatgagcaac ctgacttatt tttgcaaaag ccattatttc
    28021 aaatcctcca aaaattggta aagattagtc aagcctttac ccatgacagc aatttcttcc
    28081 tcgctcatgc catcaataat tttttctacc atggccttgt ggaagcgttt atgcagtcta
    28141 tgaatcaagc gacccttctt tgtcaaatgc agatgcacca cacgacgatc ctgttctgac
    28201 cgaactcgct caatgtagcc cttacgctct aaattgttca aacttgtcgt aacagttcca
    28261 agagttacca tcaactcttt tgacacttga cttggagtca catctggagc cttaccgatg
    28321 acatcaatcg tatgcatttc tttgatggag atatccttaa aacgactacc tctcaagttc
    28381 acttcctcaa ttacaaggac attgttaaat atagatgtta aatattcatt gattcgttgg
    28441 tagtccattt ttcatatccc tccttcttca aaaaactttt acaatcaaaa catttgacag
    28501 aaagaattat atcaaaattc aaagaatgat gcaagtattt tttattttcc tgtaaattta
    28561 ggtcttcttc tctccgaatg agctcgcact ccttctttga aatcctctgt ttgagctaag
    28621 gatttctgta ggttcagttc taaagtagcg taaccctgcc agtctttaaa ctggctctcc
    28681 caaaccaact tcttaatggc agcataggaa ttacttgaag cacgtcttaa ttttttaaga
    28741 agctgttctc tcgtcttttc aagtttttca gcttcagaaa cgcggtaaac caacccccat
    28801 tctagggctt tttctgccgt taaagcctct cctgtcatag ctaattgagc agcacgcgtc
    28861 acaccgatac tgcggctcaa gagatgaatt ccccctgcat ctggagccaa accaacacca
    28921 acaaaggctt ggataaactt agccttatcc gtcgccaaac agaaatctgc cgcaacagcc
    28981 atattcgctg cggcacctgc aacagcaccg tcaacttcca ttaaaacagg tttggctatt
    29041 tgcttgattt tataagaaat cgtattgacc aactctgcga tttttgtcaa tgatggaata
    29101 tcatcctcat ccactgcccg cttcatctct accaaatctc ccccaactga gaagaccttt
    29161 ccattggcat tgattaagat aaaatgcaca gctggatttt cttctgccaa agtcagagct
    29221 tctaaaatct cctcacacat gggaatatga aaaccatttg cgacctcagg acggttcaag
    29281 gtaaggattg ccaagtcctc ttcaagctga taaataatgt gttccatcag ggctcctttt
    29341 tattttataa gcagtttata ctcttcgaaa atctcttcaa accgcgtcag cttccatctg
    29401 taacctcaaa acagtatttt gagcagtctg cggctagctt cctagtttgc tctttgattt
    29461 tcattgagtg ttaaattatt tcactgtcaa agtatatctt ttttaggagc agaaagcaag
    29521 aaaaaatcga ctgaaagtgt ctcagccgat aagaaaggct cgctttaggc tagaaagcat
    29581 cttattttct tcattgaaaa aagcctttct ttctgctata atcgtataaa atacttactt
    29641 taggagttct tatgaaagtt gttaaatttg gaggtagttc tcttgcctct gctagtcaat
    29701 tagaaaaagt tttaaacatc gtcaaaagcg attcagagcg tcgttttgta gtcgtttctg
    29761 cgcctggtaa acgcaatgct gaagatacta aggttacgga tgccctgatt aaatactacc
    29821 gcgactatgt tgcgggtaac gatattagca agagccaaag ctggattatc gaccgctatg
    29881 ctgctatggt tagtgaattg ggactaaaac cagctgtgct agaaaaaatt tctaaaagca
    29941 ttcacgcctt ggccactctt cctattgaag aaaatgaatt tctctacgat actttcctag
    30001 cagccggtga aaataacaat gccaaattga ttgctgccta ctttaaccaa aatggtatcg
    30061 atgcacgcta tatgcaccct agagaagctg ggattgtggt cacaagtgaa cctggtcacg
    30121 ctcgcatcat tccatcaagt tatgacaaga ttgaagaatt gacaaacacc aatgaagtcc
    30181 ttgtcattcc tggtttcttt ggtgtcacta aggaaaatca aatctgtact ttctcacgtg
    30241 gaggttctga tattacaggt tctatcattg ctgctggtgt caaagctgac ctctatgaaa
    30301 actttacgga cgttgatggt atctttgcag cccaccctgg tattatccac caaccacact
    30361 cgattcctga gttgacctac cgtgaaatgc gcgagttggc ctatgcaggc ttctcagtcc
    30421 ttcatgacga ggctcttctt cctgcctacc gtggaaaaat tcctctggtt atcaagaata
    30481 ccaacaaccc tgaccatcca ggtactcgta tcgttctaaa acacagtaat gatgaatttc
    30541 cagttgtggg aattgctggt gactcaggct ttgtcagcat taacatgtcg aaatacctca
    30601 tgaaccgtga ggttggattt ggccgcaagg ttctgcaaat cctggaagaa cttaacatcg
    30661 gttgggaaca tatgccaaca ggtatcgacg atctttctat cattctccgt tctcgccaac
    30721 taactcccat caaggaagaa gaaatcctgc gtcagttggt tcaaaaggct aaagtagatc
    30781 atgcagaaat cgaacacgac ctttctatca ttatgattgt tggtgaaaaa atgaagagcc
    30841 acatcggagt gactgctact gcgacacgcg ctctatctga aaacaaaatc aacatccaga
    30901 tgatgtctca aggttctagt gaagtttcta ttatgtttgt tgtcaataaa gaccaagaga
    30961 aagcagcaat taaagccctc tacaatgcct tttttggaga aagtaaggaa gactaagcat
    31021 ggcccaatca cttaacaaaa cagtactcct cagtacgaca ggcacttcct acctctctat
    31081 cgctgggaaa gttgggaaat tccttgtcgg agatcaggct ttggaatttt acccagatgt
    31141 caatgtcgaa caatttatcc agattccttg gagccatatc aatcagattg gagccaatgt
    31201 cactggatgc aaaatcagcc gccacttcga agtctttaca gacaaaggaa aattcctctt
    31261 tgcttcaaaa gactcaggtg ccattctcaa aattgcacgc gacaaattgg gcaatgacaa
    31321 ggtcgtcaaa cttccgaccc tgattcagac aattagccaa aaatttaaaa atctatttgc
    31381 aaaaaaatag aaactttagt ataatcatag caacggataa gtaggttatc ttcttctttc
    31441 agaaagtctg cgggtgctgc gagcagatag gagggatagg tgaaattcta ccgttagtaa
    31501 caattacata cacaatcaag tgcacacctg tgaagttgga tggaaccgtg gccctgccac
    31561 tccaacgttt tgtcaggtgt gcttttttca taaaggagtt cttatgttag atatcaaacg
    31621 tattcgtaca gattttgaag ctgtcgcaga aaaattagct acacgtggtg tagatgctgc
    31681 tgtcttgaat gaaatgaaag aaatcgatgc taaacgtcgt aacatcttgg tcaaggttga
    31741 aactctcaaa gcagaacgta acacagtttc tgctgagatt gcccaagcta agcgcaacaa
    31801 ggaaaataca gatgacaaga ttgctgccat gcaaaatcta tctgctgagg ttaaagcctt
    31861 ggatgctgaa ttggcagaaa tcgatgctaa attgacagaa tttacaacga ctcttccaaa
    31921 tatcccagct gacagcgttc ctgttggggc tgacgaagac gacaatgtgg aagttcgccg
    31981 ttggggtact ccacgcgagt ttgacttcga acctaaagct cactgggatc tcggtgaaga
    32041 ccttggtatc cttgactggg aacgcggtgg taaggtaaca ggcgctcgct tcctcttcta
    32101 taaaggcctc ggtgctcgtt tggaacgtgc tatctacaac tttatgttgg atgaacatgg
    32161 aaaagaaggc tatactgaag tcatcacacc ttacatagtc aaccatgatt ctatgtttgg
    32221 tactggtcag tatccaaaat ttaaggaaga tacttttgaa ctcagcgata ccaactttgt
    32281 cttgattcca actgctgaag ttcctctggc aaactactac cgtgatgaaa tcttagacgg
    32341 caaagatctt ccaatctact tcactgctat gagtccgtca ttccgttctg aggctggttc
    32401 tgccggtcgt gatacgcgtg gcttgatccg tttgcaccaa ttccacaagg ttgaaatggt
    32461 caaatttgcc aaaccagaag aatcttacga agaattggaa aaaatgacag ccaacgctga
    32521 aaacattctt caaaaactca accttccata ccgtgtcgtt gctctctcta ctggagatat
    32581 gggcttctca gctgcgaaga cttacgactt ggaagtgtgg attccagcac aaaacaatta
    32641 ccgtgaaatc tcaagctgtt caaacacaga agatttccaa gcccgtcgtg cccaaatccg
    32701 ttaccgtgat gaagcagatg gcaaggtgaa actccttcat accttgaacg gttctggact
    32761 tgcagttgga cgtacagtgg ctgcaattct tgaaaattac caaaatgaag atggttctgt
    32821 gaccatccca gaagcacttc gtccatacat gggtggagct gaagtcatca aaccataaaa
    32881 aataaggttt agctatttct agctagacct tttttcgtaa ccaaatcaga taagcaccta
    32941 gtacaaagaa taaaatagtt aggcatataa tggtttcagc caataccagg taatccagaa
    33001 atggaagttt caaaattccc tgagccatct tgagcgaggt cgctgtgata atggttggga
    33061 aggtgagggc tgagaaggct ggttgaaaac cttgttttaa aatgttgggc agacgagtta
    33121 aaacaaagaa aaagaaggat tgagaagcca aaatcatgac aatcaagacc caattcggca
    33181 ggctggttcc tcctactcga actagagaag ccaagagtag agagaaagga gcacagtaga
    33241 ttccttcttg tccaagcaag gctagtggga gtggatgttt ctttaaatcg ctataaataa
    33301 ggggatagag atagaaggtc aagagaaaac caaaactcaa ggtcgcatag gcaatttcga
    33361 taatacctac cagaggatag gtcaaggcag ccactgctat ccccacatag agaaccgtcc
    33421 aacttggagt ggcatgaacc ctccgccctg aacaagcaaa cttgatggta aaaccagcaa
    33481 tcaaggtcaa atccaagaga aatgaaaacc accaaatccc ttgtgctacc aaaggaagat
    33541 aagagaatac gcgaaagaca taggtcgata aaatcatccc agccatagga aaggttgcca
    33601 ttcctgacaa aagagggggc ttggtcaatt cttgcttggt ttctttccaa ttaaagagat
    33661 gcagaattag aaagtaaatc cataaaacca aaccagtcag actaaaaaga tgggatagaa
    33721 ccggcaacgt atctaaaata agatttccag ctcctgccaa acctagcaaa caacctgaaa
    33781 atactaaggg gagttttttc atcctaacct ccaataatca tgttagtttc agtataacat
    33841 aaaagcgctt aaatgatgat ttaaaaaaac gaagtccgat tatttcagac ttcattttac
    33901 tcagatatga attaggcata aggttgcaat tctggattaa ttggtgtatt agctaagttg
    33961 ttggcatagt tacagaggat tgctaggctg acaccaaaaa ccacatccaa ggcattttgt
    34021 tgagtgtagc cagcttctaa aaactcagac aaggcttcat ctcctacacg acccttggta
    34081 ttgataactg ccaaggtaaa cttagctagg gtatccaatt taggatctgt ttcaattgga
    34141 gtacgattgc gaagagcttg aatcaagtca tcattcatct ggatttgttt gatggaaaag
    34201 gctgtgtgac ctgcgacaca gaaggcacaa ccattggtca cggctgccgt gatttgcacc
    34261 acttcacgct caacgggtgt caggctgttg cgacggtgga tagatgagac aatttggtag
    34321 gcttctaaaa cagtcggggc attggccaag agaccgatta ggttgggaat atagccattg
    34381 ttgtcttttt ctactgtttc aagaatttct ttcacttctg ctggtgctga ctctactgta
    34441 tggatagtaa atgttgtcat aagatacctc ttttcttatt attgacacta atattattgg
    34501 aaaatcttat aaaatcctga ttcctaagtt tatctaagat aaagctttat tctctcataa
    34561 gattttcgtt gttatattag tttatcacac ttccaatcac ttgcataata tatattatat
    34621 atcaggctga taaaaattat ttataggcaa aaaaatcaca cgagctgtgt gattccatta
    34681 tttgtcaaaa tactttttag tttcagcaat aacgacttgc gacaagacca agagggcaat
    34741 caagtttggc agagccatca aggcgttaac gatatctgcg ataatccaga ccatatccaa
    34801 ctcgataaat cctcctaaca agaccatgag cacaaaaacc acacggtaga gccagataaa
    34861 gcgaacccca aagaggaact caaaacagcg ttctccgtaa tagttccaac ctagaatcgt
    34921 tgtaaaggca aaaagtacaa ggaagatggt caagagggca ggcccaaagt gtgaaaagac
    34981 tgttgagaaa gctgactgag tcaaggcaac cccattcaag tcaccactcc aaactccagt
    35041 taccaagatg gtcaaaccag ttagagtaca aatgatgagg gtatcaataa aggttcctgt
    35101 catggaaatc aaaccttgct ctactggttc atttgtcttg gcagctgcag ctgcaatagg
    35161 agcagaaccc agaccagatt cgtttgagaa cacaccacgc gccacaccat tttgaatagc
    35221 catccgaacg ctagcaccag caaatccacc taccgcagca aggggactaa aagctgaggt
    35281 aaagactaaa gcgattgtgc cagggatttt tccgatatta aagaaaataa ctataagagt
    35341 tcctaagata taaatgatgg tcataaaagg aacaacagta gttgaaacct tagaaataga
    35401 cttgagtcca ccaaagactg caatcgctac aaagacagac aagacgagag ctgtgatgac
    35461 tggcgaaatc gtcgttgtat tttggataga ttctgtaatc gagttgactt gggtgaaggt
    35521 tccgattccc aagagagcaa ccaatactcc tgctactgca aacaaaacag caagtggtcg
    35581 ccacttttct cccatcccta gaaggatata gtgcatggga cctcccgcta ctgcaccatg
    35641 gtcgtccttg gtgcggtatt tgatggccaa gagtccttcc gcatacttgg tagccattcc
    35701 aaagaaagcc gccatccaca tccaaaatag agctcctggt ccaccaacct tgatagccgt
    35761 cgcaactcct atgatatttc ctgttccaac agttgatgcc aaggctgtac acagagctgc
    35821 aaaactggat acatcaccat gtcccttatc ctggataaaa ataagctgaa aggccttggg
    35881 cagacgcaaa acctgcaaga gtcctagccg catggttagg taaatccctg ttccgaccaa
    35941 taaaatcaag aggggcggtc cccaagcaaa atcatcgatt gatttaagca attctaacat
    36001 ttccttctcc tatcgtttca accccaaaag aaagagcaca tgcaagatac atgtactctg
    36061 gaatgcttag ataaatgcta aaaagcggtc tatcctagct ctgtcctttt acctgagagt
    36121 ttgagcagtt gcctgccttg ccccttcggt gcctttacgg tctctccaga gttccgtcca
    36181 tttacagtca tggaaaatca aacgattccc cacttctatt aaacttcatt cggtgttggt
    36241 atttaattga ttctaatttt acaaaaaatg ttggcttttg tcaatgtgtt tattagtaaa
    36301 aattagttca acagttttta ctttataaag tccagaatac tgctatcctt taaaagtgac
    36361 aatagtcgca ccactgcctc cagcattttg tggggcatag ccgaaactct tgacatgttt
    36421 gtttctttgc aagtatttgg taactccttc acggatgact cctgttccga taccatggat
    36481 gatatcaact tgagccatat tgttaagcaa ggcttggtcg atgaaggtat ctagctcatt
    36541 catggcttct tcatagcgct tgcctcgaag atccagtcta gcttgaggtc ctcgcccaga
    36601 agttcgtttc acaacattga cctgtttctt cttgactggt ttttcttgct gggcttgaac
    36661 aagatcaaac tctttctctt ccaaggtcat cttaatcaag ccaacttggg cttcccagcg
    36721 gccgtccttg agttgactgg tcaaggtacc acgctgacca taactgagaa ccacgatatc
    36781 atctcccacc tttggagctc gttttttctt ggccttttga aggaccttgt ttttagacaa
    36841 gtccactttt tcaggagcca attttttcaa cttggccttg gcttcaatga tttcgtgggg
    36901 cttgagttgg gatttactgt ggagattttt gagaatctgg tcactttcac ttagggccat
    36961 atccacaatc tcagcagcct gttcacgcgc cttgttaagc tcggtttcct tttcacgatt
    37021 aagctcgttg tagagttttt ttagcgcacg gttcatcttg agattttctt gctccacctc
    37081 acggatattg tccaaacgtt tgcggctttc cagcgtctgc tcttctaatt gctcaatgat
    37141 acgattgacg tcattgtcct gatcgatctg ctgactggca tctcctacga taacttcaga
    37201 taggcctaga cgtttggcaa tttcaaaggc attacttcgg ccaggaacac cctgcataaa
    37261 gcgataggtc gggcgaagag ttgcagtatc aaactccata ctggcatttt gcacaaaggc
    37321 tgtctcaata ccgtaggcct tgagttctgg atagtgggtc gtcgccatgg tcttgatttg
    37381 acgcaggcga aggtcctcca gaatagccat ggcaagggct gctccctctt ggggatcagt
    37441 accagccccc aactcatcca aaagtaagag tgaatgttgg ttgaccttgc caagaatatc
    37501 cacgatattg gtcatatggc tagagaaggt agacaagctc tgctcaatag actgctcatc
    37561 tccaatatca gcaaagattt cttcaaaaat accaacacga cttcccttgt ctgctaaaat
    37621 cggcaatcct gactgggcca tgacctgtgt caagcccaga gttttgagca tgatggtctt
    37681 cccacctgta ttgggacctg taatgacaat agctgttaaa tcttgaccaa aatagacatc
    37741 atttgcgacg gcatttttga ccaaaggatg gcagacatgg agcagttgaa tctcttgatt
    37801 ttctgacagc tgaggcacga ctgcttgtct ttcttggata aatcgaacct tggcacgaat
    37861 caagtccaga tgaccgataa tccaagcgtc attagcaatc tcagccgcat gagggcggac
    37921 acgctcagaa atttcttgga gaatgcgaag catttcatag cgctcatctg ctcgcagact
    37981 agcaatttct tcgctcagtt tgactacctc acgtggttcg atatagacgg tgtttccact
    38041 agcagaaata tcatgaacga cacctgcaat cttattgcgg taggtgtttt tgactggtaa
    38101 aacctgacgg ccatttctgc tagcaacaat tccttccgtc aacatctgcg ctttttgctt
    38161 gagtaagtct tgtaaaacat cgcgcacctg actctcgcta tcatgtattt ttcgacggat
    38221 tcgcgccaat tcttcactgg caaaattttc aatgaaaccc gcatcattaa aggcctgaag
    38281 atttccttgt aattgcggaa aatcatgtaa tttctcaaac caaagggcta attcttccaa
    38341 gctgacattt tccagattgg cgtaaaaatt ttgaagttct cggctggcaa gaagcacgcg
    38401 tttcaagagt aggaactcct cgatattgag atccgctccc atctccaacc tcttgcagac
    38461 tcctgcaatt tccttagttg agagaatagt aaaatgcggt tgctcgacga aaagagcctg
    38521 catttctttc atctcagcaa aagcctgttt gattttatct gctttggcag tcggagccag
    38581 ctgtctcaat tgctccaagc cctgctcggt caacaaatga ggctcaaaca aggccttgac
    38641 cttatcgaac tctaatgttt ctaatatttt cttattcatc tttatttcct tgtctttgaa
    38701 tgtctaggtg tggtagcttt ttacattctg atagattcga gtcaaatctg taaacaaagg
    38761 gctaggaaaa ctcctgccct ttttatccga ttaaatttgt cacccagatt tgtttgagcc
    38821 aactggttgt taccggtatg ctctggatga tgtgttttgc gacgatactc ttttcaagag
    38881 gattttgtat aactgccatg gggatggtcg ccaagattgt caaggccatt tgcaagacaa
    38941 ataaggtcac caacatggac aagatacctg ctgaaacttg gaacaactta ccacccagtt
    39001 ttttgctagg aatcaagtgt aagagaagac caagtaaacg accaatgcta tagacaatcc
    39061 caaatacaag caagtagccg atacctgcat aaaagacctt atccagctga aagagttgat
    39121 ccgatgggaa aaagaaagtc ccctgacctt cctgcgaatt tgcataaggg agcaataaat
    39181 ggaattgctc tccaagcccc ttataaaact ggccagccat aaaagccgat gccatggctg
    39241 aaatcaggta ataaacctgt aagagcaggc ctctccgata gccgatataa aatccccaaa
    39301 ccaagaccaa tagaagaagg aatgaaatca taaggaatcc tcaatcttgc tctgttcttg
    39361 cttacaagtc acaagcttgt gacggagttc ttctagctct tgctccttat cgtcaaattc
    39421 aatctcacgg ctgagctgag ttgataaaca gttgactgcc aacaaaagag cgattgtttc
    39481 atcatctgcg ctaggcattt gttctttaat tgcttggtat ttttctgtcg caaccttagc
    39541 gatttcctcc ataaaaaggt tgtcatgttc gcttgtcaag gttaacgatt ttttcccgaa
    39601 tgtaaatttg aatcgattta gatttgccat aaaattcacc tcacgatatt ataccaaaat
    39661 tcgctaattt tgtcagtttt tacaaatttt actgcttttg tggtacaata gaaactatgg
    39721 caagtatcac actcacacca agcgaaaagg atattcaggc ttttcttgaa cactatcaaa
    39781 ccagtctggc tcctagcaag aatccctata tccgctactt tttgaaacta cctcaagcaa
    39841 cggtttctat ctatacttct ggaaaaatct tgcttcaggg tgaaggggct gaaaaatacg
    39901 ccagtttctt tggctatcaa gctgtagagc aaaccagcgg acaaaatctt cctttaattg
    39961 ggacagatga ggtgggaaat ggttcctact ttggtgggct tgcagttgtg gctgcctttg
    40021 tcacacctga ccagcacgac tttttacgaa aactcggtgt gggggattct aagactctga
    40081 ccgaccaaaa gatccgtcag attgctccta ttctcaagga aaaaattcag caccaggcac
    40141 tccttctctc acccagcaag tacaacgagg tcatcggaga ccgctacaac gctgtttcgg
    40201 ttaaggttgc cctccataat caggctatct atctcctcct tcaaaaaggt gttcagcctg
    40261 agaaaattgt gattgatgcc tttaccagtg ctaaaaatta tgacaagtac ttggcacaag
    40321 aggccaatcg tttcagcaat tctatcagct tagaagaaaa ggctgagggc aaatacttgg
    40381 ctgtcgcagt ttcttctgtc attgcgcgtg atctctttct ggaaaatctt gaaaatttgg
    40441 gacgagaact gggttatcag cttccaagtg gagctggaac ggcttctgac aaggtggcta
    40501 gccagatttt gcaagcctat ggtatgcagg gactcaactt ctgcgctaag ctacacttta
    40561 aaaatactga aaaagcgaaa aacgcttaga aaggtaagtt atgaatttat ttaaaaattt
    40621 cttaaaagag tggggattat tcctcctgat tctgtcatta ctagctttga gccgtatctt
    40681 tttttggagc aatgttcgcg tagaaggaca ttccatggat ccgaccctag cggatggtga
    40741 aattctcttc gttgtaaaac accttcctat tgaccgtttt gatatcgtgg tggcccatga
    40801 ggaagatggg aataaggaca tcgtcaagcg cgtgattgga atgcctggcg acaccattcg
    40861 ttacgaaaat gataaactct acatcaatga caaagaaacg gacgagcctt atctagcaga
    40921 ctatatcaaa cgcttcaagg atgacaaact ccaaagcact tactcaggca agggctttga
    40981 aggaaataaa ggaactttct ttagaagtat cgctcaaaaa gctcaagcct tcacagttga
    41041 tgtcaactac aacaccaact ttagctttac tgttccagaa ggagaatacc ttctcctcgg
    41101 agatgaccgc ttggtttcga gcgacagccg ccacgtaggt accttcaaag caaaagatat
    41161 cacaggggaa gctaaattcc gcttatggcc aatcacccgt atcggaacat tttaagaaac
    41221 ctaagaggcc gagaatcacc aatctcagcc tcttcttcta tcgtgagaaa atgattggta
    41281 actatctaaa cttaccagaa cagaaacacc tcaactctca cctattcatg caaaggaatt
    41341 ttatggaagt ttatttttca ggaaccattg aacggattat ttttgaaaat cccagcaatt
    41401 tttatcgcat cctcctccta gaaatcgacg atacggacgc agaggatttt gatgattttg
    41461 aaatcattgt cacaggaacc atggctgatg taattgaggg cgaagactat actttttggg
    41521 ggcaaattgt ccagcactcc aagtatggag aacaactgca aatcagtcgt tatgatcgcg
    41581 caaaaccaac tagtaagggc ttggtcaagt acttttcaag tagccatttc aaggggattg
    41641 gtctcaagac agctcagaaa atcgtggata cctatggcga aaataccatt gacgaaattt
    41701 tgcaacaccc agaaaagtta gaaggcatcg caggattctc tgccaaaaat cgcgaggctt
    41761 tcgtctccac tctccgtctc aactacggaa cggagatggt tttgtccaaa ctagccaact
    41821 acggcattcc caacaaacta gcctttcaga ttcaagactt ttacaaggaa gaaacccttg
    41881 atgtggttga aaattatccc taccagctgg ttgaggatat caagggtttg ggctttacaa
    41941 ttgctgacca actagctgag gaactaggca tcgaaagtca ggctcctgaa cgcttccgcg
    42001 ccggtctagt tcacagtctt tttcaggcct gtatggaaac aggggacacc tatgttgaag
    42061 cacgggattt gctggaacaa acccttactc tccttgagtc ttcccgtccc gtggaactag
    42121 accccagcca agttgcccaa gagctctcct acctgatcga agaagacaag gttcagcaga
    42181 ttgataccaa gatctttgac aacagcctct ttttcgctga ggaaggtatc cgcagtcact
    42241 tgattcgtat ccttgaaaaa ggaaaacaga agagccaaga tttagaaact attcaaaagc
    42301 atatcactac tgtcgagcaa gagctgggaa ttgagtatga taacattcaa aaacaggcta
    42361 tctgtgatgc tatccagaat aaggtcttta tcctgacagg tggacctggt actggtaaga
    42421 caactgttat caatgggatc atcgctgttt acgccctgtt agagggactt gacctcagga
    42481 agaaaagcaa tctgcccatt cttcttgctg ctccaactgg acgagccgct cgccgcatga
    42541 atgaattgac aggtttgcct agcgcgacca tacaccgcca cttgggaatg acaggtgacg
    42601 atgataccag tcatctggaa gattacctag atgctgactt tatcatcgtg gatgaattct
    42661 ctatggtaga tacttggctg gccaatcaac tcttctccaa tatctcttct aacagtaaga
    42721 tcctcatcgt aggtgacagt gatcagctac cttctgtcag tcctggacag gttctagcgg
    42781 atctgcttca tattcctttg attcctcaga ctcgcttgga aaaaatttat cgacaaagca
    42841 aagaatcaac catcgtcacc ctagctagtc agattcgaca gggcatcttg ccagctgatt
    42901 tcacccaaaa aaaagctgac cgttcctact ttgaaattgc tagtggccat attcctgcca
    42961 ccattgaaaa aatcttaggt gtcgccctca gaaatggtat tcctgcccgt gatatccaag
    43021 ttctggctcc tatgtaccga gggacggcag ggattgatgc tatcaatcag ctcatgcaag
    43081 acctgctcaa tcccccacaa aaagatcaac tcagttttga agctcctcag tgccactatc
    43141 gtaagagaga caaggtcatt catttggtta acgatgctga aatcaatgtc tttaatgggg
    43201 atttaggagc tatcacagac ctgattcctg gtaaatacac cgaatcgaaa caagacgaga
    43261 ttgtcattga ttttgatggt aatgaagtct cttacccccg taacgaatgg tacaagattc
    43321 gcttggccta tgccatgagt attcataagt ctcagggaag tgagttccca gttgtcatcc
    43381 taccgattac tagtgctagc cgacgtatgc tggagcgaaa tctcatctac acagccatta
    43441 cacgtgccaa aagcaaactc atcttactag gcgaattaca agccttcgac tatgctaccc
    43501 aacacatcgg aactgcccga aaaacctatc tgattgaacg cttcagtgat ctactggaga
    43561 atgttgaaga aaagcaacaa gctgtctctg aaacagtcac atcaagtgcc tctgaacaat
    43621 cctacatcct aaccgaagaa aactgggacc gcatcccagc catgattggg attacagaca
    43681 cagacctcaa agagattttt ggaaaatagt gcatcagaaa acccaccaat tcaggtgggt
    43741 tttctgttta ttaagattat tttactgttg ctgtgcttgt gatcaattca acagcttttt
    43801 tgatagtgat atcatgtttc aacatgtcag ctgaaagcaa gttttgaact tgtgcaactt
    43861 ccatgttgta gtctgctgcc aattgctcaa cttctttttg gatttcttct tctgaagcat
    43921 caaatccttc agctttggca actgcttcga taacaaggtt agtcttagta cgtgactcag
    43981 cttctgcttg gtattggttg tgaaggtctt cttgagtagt tccagtgatt tggaagtaca
    44041 tgtcagggtt gatcccttga cgttgcaaat tcccaaggaa ttcatttact gaacggtgaa
    44101 cttcttcatg gatcatttct tctggaagtt ctacgatttc agcattttct acagctgtat
    44161 caattgctgc accttcaact gcatctttgt aagcttcttc tttagcagca gccaattctt
    44221 tgcggtattt ttctttcaag tcagcaagtg tttcaacttc ttcatcaatg tcttttgcaa
    44281 gttcatcgtc aagagctgga acttctttag cttttacttc gtggatagtt gtcacgaatt
    44341 tagcttcttt acctgcaagg tcttctgctt ggtagtcttc tgggaatgtt acgataacat
    44401 caacggtttc gccagctgag tgacctacca attggtcttc gaaaccaggg atgaattgac
    44461 ctgaaccaag tccaagtgag aagttttcac cttttccgcc gtcaaattca acaccgtcga
    44521 tagaaccaac gaagtcgatc acaacagtgt cgccgttttc agcagcagct tccttgataa
    44581 ccaattcagc caggttgttg cgttcgcgtt cgatacgctc ttcaacatca gcgtcagtta
    44641 cttctttttc tacatcaact gatacttcaa ggtttttgta gtcacccaat tttacttcag
    44701 gttttgtaac gacttcagca gcgataaccc agtcttgacc tttttccatt gaagttacgt
    44761 caatttttgg ttgggcaacc acttcaagac cagcttcttt tacagctgct tcataagcgt
    44821 ttggcaaaag agcgttcata acgtcttggt aaagtgattc ttcaccaaat tttttatcga
    44881 agataggacg tggaaggtga cctttacgga aacctggaac attaagagat ttcttcactg
    44941 acttgaagac acggtccaat tctggtttga tttggtcttg agagatagtg aaagtcaaga
    45001 caccacggtt tgtttctttg ttttcaaatg atacagacat tctgtcattt ctccttaaaa
    45061 ttttttaaat acagtctatt ataacatttt ctataaataa aatctagact aatgcagcca
    45121 attatagaca acaagccttt ttcctaacta aaacactcat aaagtataga caattttaga
    45181 ctactttgga tatcttttgg ttagtcattt tccaaaacta atttatctaa tttttcagtt
    45241 atatttgact tcatcttctc agtaacatga gtataaacac ttaaaatctt tgtttcatta
    45301 gtataatcta cacgatccat tatcgctgtt ataggtactt ccaattcagc aagcaaactt
    45361 atatgcgaat atctaaaaac atgagcagtc aatacaaaat catctttctt aatttcactt
    45421 ttcctaaaac tgtttttcaa aaagtaattg aaacaagtta tcattaaggg agaaccatta
    45481 tttgtaaata tataatcacc ttcttctaaa agaagatatt cttttaaaat ttctaacact
    45541 ctatcactaa taccaattac tctatatgaa gctaaacttt tcggactatc caaaaatact
    45601 tcgttattgg acgattacat tatagtaaaa cgaaataaga acaggacaaa tcgttcagga
    45661 cagtcaaatc aatttctaac aatgttttag aagtagaggt gtactattat agtttcaatc
    45721 tactatacaa gacgaaattg aagaaatctt tccattataa aacgcatatt atcaaggttt
    45781 ttaaacacct aataatatgc gttttttcct attttaaaga cttttatcca cctattatac
    45841 aaagttaagt atccatcaca gtttacaatt agccttcatt acctaaccca ttcaccatcc
    45901 ttcaagagag taacctttaa gaaccgaata aataaatggc aaatttcgtt catttaattt
    45961 aactgcactg ttaatttgat tagctccatc tcctaaagta tgcactccta ctacacggtg
    46021 actagcatca taaactgtag atccactaga cccctctaaa gtatcaactt ggtaatccaa
    46081 gaacatgcca tcatcactta aaacttgttt tttatctgta tacatttgat gaattttaaa
    46141 attatatgat agatagcctg tgatagtcac agttattcct gtcaaatttt tttgactagt
    46201 aggaagaccc aaagtcccta attttgcacc aatgggctct tctagaatta ataaagccaa
    46261 gtcatattcc cttgcatcct tagaatttaa atttctaaat tccttcaaat aacgaacttc
    46321 ctttactttg atctttccaa atagttcttg acttggacta acagccggaa gaacataaat
    46381 atcatccgct tctttcccat agtcatgtct gtagtagttg tgagccgctg taattaacac
    46441 attatcttta ataaaattcg cacttcccct cccttctaca ttcattccat cggctactcc
    46501 ataatactta gataaaacca tagctgaagt cgaatacgga tactgtaaag tattatcaat
    46561 tttaatcaaa tcatcattac cgataatact tctgattgct tttggtagta tgaaccatac
    46621 gttggtgaaa tctcagataa tgaagaatca ttagactctg gacctttttc tagtgtctca
    46681 cttacctcat attcttcacc tgtactagaa ataacactca aagcagatac tgtcgataac
    46741 tggctagcca ataaagtact cgcaataatt gaaataccca attttttata aacagttttc
    46801 ttcattattg tatcctccta atgtaattat agcgtactat tctaaatttc ttaatctact
    46861 atagaatcaa gaaatctacc accttcttta aataccctcc attatcacat aaacaggtaa
    46921 acttttcaat taatgactgc gcttttcaat cacgctagag gtacttgctt gcttctttga
    46981 tactaagttc agccattctt tccttgtttt tctcaataaa gcatgttacc caagtgggat
    47041 tcgttttgga gtagtctcgc agagtccagc caatggcttt attgataaaa aattctgttt
    47101 ggttcaagtt atgaagtaga atcttttcca ttaattgagt attggtcttc tcttttctta
    47161 acaactggtg gtcaatagcg acacgtctca gccagatatt atctgatagg ctccatttta
    47221 tactcaatga aaatcaaaga gcaaactagg aagctagccg cagattgctc aaaacactgt
    47281 tttggggttg cagatagagc tgacgtggtt tgaagagatt ttcgaagagt attaagatta
    47341 tttcttctag ttcagagtgt tcatacacca aactccctac tactcgatct aggatatcta
    47401 ccgtgtccca ccaggatttt gtcacgacta actgctctag cttaggcaaa tcggtttcct
    47461 ttagataaga ctgcattgct ttcaaatagt tagcagccac atattggtat tctctaggat
    47521 ccttttccca gcaagtgtct gcaaaatccc aatcgataat ctttgttttt ttcgcttctg
    47581 gaaaatattt ttatagagtt tatttctttc aggcaccgca atacctagaa aagaaaattg
    47641 atggcgcata taggcttcca tggaccttgc ttttttagag tcttttgctg cttctagctc
    47701 ctcaagtaaa tctgctaaac tcatctaaaa ctcctcttgc cccaccaaat ggtgctgaaa
    47761 ggcatagaca gccgcctggg tacgatcgct gacttcaagt ttggcaagaa tattggacac
    47821 gtgggtcttg accgtcttga gagagataaa gaggtcatct gcgatgcgct gattttcgta
    47881 gcccttggcg atgagttgga gaacatctcg ctcacgcgca gtcaattctt catgaagttc
    47941 catatgattg cggtggtatt caaccttctt gctaacctct tgctcaatgg ccagctcgcc
    48001 agcagctacc ttactgacgg catgaagcaa ttcatctgca ctagaagtct tgagcatata
    48061 gcctttggca ccagcatcta agactggcat gattttttca ttgtccaaat aagaggtcac
    48121 aatcaaaatc ttggcttcag gccattcttt aaggattgct aaggtcgcgt caatcccatt
    48181 catctcaggc atgacaatat ccatgacaat gacatctgga cgcagttcca aggccaagtc
    48241 aatcccttga gacccgttgg acgcctcacc cacaacttct acatcgtctt ggaggtcaaa
    48301 gtagcttttc aagcccaatc ggaccatttc atggtcatct actagtaaaa ttttcatctt
    48361 tactccttta tcattcctta tctaacaggg gaatacggat atcaaccgcc agcccttgct
    48421 tgggagctgt caagagttga actgttccag ccatatcttc aacccgctcc ttgatatttc
    48481 gcagtccata actcaagtcg tctaagctcc ctaactggaa accaatccca ttgtccacca
    48541 ccttcagttg caattcaaca tctgtctgat agaggtagac atctaggcaa gatgcctggg
    48601 catggcggag ggtattgcta atcaactctt gcaggatacg gaagatatgc tcctcgattt
    48661 tcttaggcaa tttcgtcata ttctgcttga gactaaccct aagatcactc ttgtcctcaa
    48721 gctcttttaa gagaatttga atcccttcta tcaagctctt ctgctccagt tcaactggtc
    48781 gcaaatgcaa gagcaaaacc cgcaaatcct tctgggcagt ttctaaaata gctgtgacac
    48841 tctgcaactg gatctgcatc ttttctctat ccaatttcaa agcctgctga ctgatacccg
    48901 ataaaatcat gtgggccgca aacaactcct gactgactgt atcgtgcaaa tcccgagcaa
    48961 ttcgcttccg ttccttctcg atgatttcct cttcctgagc aaggctatga ttttcagctt
    49021 tttgaagagc ttctgtcaaa aggttaagtt tacctgataa ggacttgaaa ctggcatcca
    49081 aatctggatc tgcaacctga accacttctt gccctgccaa taaacgcttg agattagcct
    49141 gcatttttct tagagaaagc tcttcgatcc ctcgccaaaa cagggctaag agacaggtta
    49201 tggacatgct gaaaaccaac aataaaaaga caaatttttc tgttttttcg acatcgtgca
    49261 aaaagataga ccagtcaaaa tcaagtattt ccagcaagct gtgggagaaa aaaaagacaa
    49321 acaggaagga ggtgagagca ataatgacat aggcttgttt tttcatcctc taaccacctc
    49381 cacatcacca atcatagtgg tcaagaaaat cttgacactc ttgttactct tgagatagtc
    49441 ttttgtttct tgatgatagt gttcattgcg gagggctcgc ttgggctggt tgaaaaaagt
    49501 caaatcccca tagagacagt taacgctgag actgacttcc acatctacag gtacgatgat
    49561 tttggtcgtt cctaccatct ttctgaggat aatgacattg tcatgattgg ttaagatgac
    49621 cctctccaga tgaatagtgt ccttgcccat gaagcgaaag agattgatat catcgaattg
    49681 gcaagtctgg tagcttgaaa aatgatgaag atttccaaac caacgatttt tctccttctt
    49741 aaccgtcacg acctcttcaa aaaccaaatt ggtctgctct ttttcctggt tcatcatcgg
    49801 gtaaagaaga aagaggctat agataaccgc aacaaaaata gctagaatca caaaaggatt
    49861 gagcataacg atgaaaaaga agagaatggt tgccgctact aaaagaagat tatttccctc
    49921 tttaccagtg tagtagcgaa tcaaaagcaa aaagaggaat agtatcagca gaaaacgcga
    49981 aaaatgctct gataccatca aaatcagagc tcctgtcaga agacaggctt cgataaataa
    50041 aaagatttta aattttctca taggttcatc ctctcccttc tattttatca caattcaaaa
    50101 aagtcacctc agtctgagga tggaaaaaag gcgctggtta cgcctttttc atctgatcct
    50161 ttgcttcttt taattttcca taaagaagat agtctacttt ttgtagatct gctatggtgg
    50221 cacagttaag ggaacacata atcaagcgta ggtctgcttt ccagccttgg acaatgccaa
    50281 tcacttcttc aactgtgtag gtttcaacca attccagaac ggttcgtgac aatcccacag
    50341 acttagcacc aaaaaccaag cacttaatca tatccagcgg attccgaacc cctccactaa
    50401 ccaagagttc gaccttatct ttccattctt gggcattgag aagggcctgc atggtagact
    50461 gaccccattg attgaggtaa tcacgctggc cactacgacg gttttcgata taggcaaagc
    50521 tggtgccacc acgacccgat aggtccactg tacgaacacc gaattcatag gctctttcga
    50581 ttgtcttggc atccattcca aagcccactt ccttgaggac aataggaacg ggaatttgct
    50641 cgctataatc tgctagatgc gattgccagc ttctaaactt cctttctccc tcgggcatga
    50701 gtaattcctg catgacattg acatgcactt gcaatagaac aggattcatc tcttctacag
    50761 tctgaagccc taactcgaca ggcttgtcca atccaatatt ggttccaagg aggagattgg
    50821 gatgactaga cttgacagaa aaagaatcat ccgttggatt tttgagggct gcgctataag
    50881 aacccgttac aaataaaata ccacaggatt ccgccacctg agccagcttt tgattgattt
    50941 ctcttccctt attacttcca ccagtcatgg cattgatata aaaaggaaag tcccactttc
    51001 gaccagcaaa ctctgtcgaa agatcgattt catccagatt gtaaagaggc aaggaagaat
    51061 gaatcagctc cacctcatca aagctattat aggaactttt ctgctcaagg gcatagagga
    51121 tatgctcgtc cttacgattt gtcgtcatgt cctatccttt cttgatataa gagctcaatc
    51181 cccagatcgg cccaacgatt ttttaaggtt tcggttgatt gcgcatcaaa actcaaggcg
    51241 atgccacagt caccaccacc agcaccacta ctcttggcaa cggcctgcaa atcttgactg
    51301 gcttctttca actgtctaag caaaggcgtg taaatatctg tactcaagcc ttctaaaagc
    51361 ttgctggcta cttctacttg ctcgataact ttttcggctt tcccctgctc caaggcttcg
    51421 accaaagaaa ccaccgtttc ttttgaggaa cttaaaaaat tttgattgat attttgcttg
    51481 atttgctgga ccatgtgact cgatacagcc acttccttgg tccatcccac taagaaatca
    51541 cattctaaag ttggtttcac ttgtgagata aaaaatcccc aatcacgctc cagaactgtc
    51601 gccaagtttt cttcttctaa ccaagcagcc accttctggc gatcaaatga ctggtagaga
    51661 accaaatcct ctgccacaat acaggcaagg tcgcccatgg aaccattgtc tcctcgcttg
    51721 agcaagacag cgctagtcag cttgaacaag agattctgat caaccgaaag attatagaga
    51781 gccagtaaag ccttgacaac caagacaacg acgctgccac tagaacctag accaaacttt
    51841 ttcccttctc gttccatttt gccacagatt tttagggaaa aaggtcttaa attctgaccg
    51901 cgaacagcga ggaagtctcc catcaaagca atcgtttctt gaatcaagct gtagtcagga
    51961 ttaggcctta agtccactgc gaaatcaaac atatctgaat agatacggta gctgtcagaa
    52021 aaagcaattt cagccctcat atagatggga atatccttta tcaaagctaa ctgccctggc
    52081 tctaaaatag catattcacc tgcccaatag agttttccgc aagttttaac agcaatcatc
    52141 ttgactcaaa tcctttgttt ttgacacaat caagcgataa cgctgaccga aaatttctga
    52201 taaatgctcc aagtctttct cctgacagaa gaccttaaca ttgggaccag catccatggt
    52261 aaagtagcag gcctctcctt tctcacgaag ctggcgaaca aagtccatag cctcataaga
    52321 ggcatccgtc agataagaaa aggctggact agcagtcttt gtcgtagcat gcatagccag
    52381 ggcatttttc tccgttaatt ctccaatctt ggcaaaatca ttttccttga gataaatcag
    52441 catatcctga tagtccttct cagactgacg aacccagtcg tcgaaagtcg tcgaggtttc
    52501 cacacaaagt ttcatcccgt cacggctaga gattggtttt ttcttgtcct ctagcaccaa
    52561 cataatcata gctagtttca agtctgtctc tacagggtaa atttctccac tatccttatc
    52621 ccaggctcct agtggtccat aaaaactccg agaagaagag cctgaggcaa acttggcttc
    52681 ctgcgctaac tgacttctat ccaatccaag cttgaaataa gcattacaag ccttgaccag
    52741 ggcggacaaa ccactagaac ttgaggacag gcccgctgcc gtaggcatat tgttttgagt
    52801 atcgatacgg acaaagccct caccagctgg acgataacgg tcaataatct tactcatctt
    52861 ggcatgctcg acctcatttt gtagctgacc attgatgtaa aattcgtcag ctgttacatt
    52921 ggctggtaaa ggcgacaagg tcgtctctgt atacatattt tccaaagtta gagaaatact
    52981 gctagtagca ggcaccatct ctttttcttt tttctttccc caatatttga taatagcaat
    53041 atttgcgtag gaacgtactg ttacaggctc tctatccatg tctgaacagc tcctttctct
    53101 tctaatcttt ctgctagttc ttgtgcgtgt gtcaaattgg ttaccaaggc tatgatacaa
    53161 cctcctagcc caccaccgct catcttggca cccagagcac catggctaag agtcgtttca
    53221 accaaaaagt ctgcctcagg gctactgact ccaatttctt ttaaatgtaa atgcgcttga
    53281 ctgaggattt gtcccagtcc ttcagcatct ttttgtgaaa tcgcaacttc tgcttgctgg
    53341 gttaattctc ccaaggcatg caaaaacggt agggcatcct tgcccttatt ttgaaccact
    53401 tggatggctt cacgagtatg accataaaca cccgtatcgg caatcaccaa ataggcggat
    53461 aaatccatct caagttctgt aaatcctacg ttcttgataa agcgaatagg ttggtcactg
    53521 agacaggtct tagcatccaa accactagga ttcatatggg caatcatttc agctcgattg
    53581 accaagattt ctagtacatc atgaggcaga tcagcctgat agtagtcaaa taccgcacga
    53641 atggccgcta tgctgatagc cgctgacgaa cccatccccc gtttctcagg gatagccgag
    53701 tcaatctcac aacgaatgca ggcttctgtg atatccaaat actccagtga ggcataaacc
    53761 gccatggaca aggtatcctc ctcataaagg cgccaaggac tctctgcagg aactacctta
    53821 caggtcacct ccacctccaa aagaggcagg gaaatggcag gataaccgta aacgaccgca
    53881 tgttccccta ttaaaattat cttactatgt gcctgaccga caccaacttt ttttgtcatt
    53941 ttttcctttt actagacgaa aaaacgtctt atttttcata caagtattaa ttctttccta
    54001 tctattttat tatattttca caaaaaaagc gattgtttcc attcacaatc gcttctttca
    54061 ttaccgaacc cattcaccat tataattgac agaatagcca tctacaatcg tattcactgc
    54121 caaggcacct gagctataag cgtagtacca tctgccattg acctggaacc aacctgtcgt
    54181 catagaacga tgaaagaaac tccataccat taagtaaaga ggaaagtcat gagggagcat
    54241 gcgtcattga caacctattt tagtgacgta caaagtttca ttaacaagta ctcgtttcgg
    54301 ccatttatag gtgcggtgtt tgcagaaata gggttcaatc ttcgcccatt cttgatcgtt
    54361 taaatcagta tcatatgctt tgcgtgtcat aactctagtt taacattttt ttgtgaatac
    54421 aggttctaaa taatcgacca cgaaaatttc ttaagtggaa aacgccttat gaagtatgct
    54481 acgggaaact tatgcactta atttgacaac tcaatatgta aaaatatata ctata
//
LOCUS       DAVBNO010000009        49378 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVBNO010000009 DAVBNO010000000
VERSION     DAVBNO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49378
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.009"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(93..551)
                     /locus_tag="VQ846_000537"
     CDS             complement(93..551)
                     /locus_tag="VQ846_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET3075173.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(548..988)
                     /locus_tag="VQ846_000538"
     CDS             complement(548..988)
                     /locus_tag="VQ846_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3075174.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1194..3143
                     /gene="mutL"
                     /locus_tag="VQ846_000539"
     CDS             1194..3143
                     /gene="mutL"
                     /locus_tag="VQ846_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET3075175.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVDQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(3468..3935)
                     /locus_tag="VQ846_000540"
     CDS             complement(3468..3935)
                     /locus_tag="VQ846_000540"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET3075176.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(3936..5141)
                     /locus_tag="VQ846_000541"
     CDS             complement(3936..5141)
                     /locus_tag="VQ846_000541"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET3075177.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(5161..5796)
                     /gene="ribE"
                     /locus_tag="VQ846_000542"
     CDS             complement(5161..5796)
                     /gene="ribE"
                     /locus_tag="VQ846_000542"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET3075178.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(5781..6881)
                     /gene="ribD"
                     /locus_tag="VQ846_000543"
     CDS             complement(5781..6881)
                     /gene="ribD"
                     /locus_tag="VQ846_000543"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET3075179.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAIIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRKSPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(7015..7129)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            7286..7879
                     /gene="ruvA"
                     /locus_tag="VQ846_000544"
     CDS             7286..7879
                     /gene="ruvA"
                     /locus_tag="VQ846_000544"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET3075180.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQED
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            7889..8452
                     /locus_tag="VQ846_000545"
     CDS             7889..8452
                     /locus_tag="VQ846_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET3075181.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            8449..9126
                     /locus_tag="VQ846_000546"
     CDS             8449..9126
                     /locus_tag="VQ846_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET3075182.1"
                     /translation="MMSNKNKGILIFAILYTILFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(9263..10294)
                     /locus_tag="VQ846_000547"
     CDS             complement(9263..10294)
                     /locus_tag="VQ846_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET3075183.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLSHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIKTGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(10549..11232)
                     /locus_tag="VQ846_000548"
     CDS             complement(10549..11232)
                     /locus_tag="VQ846_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET3075184.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGSDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(11244..12188)
                     /locus_tag="VQ846_000549"
     CDS             complement(11244..12188)
                     /locus_tag="VQ846_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET3075185.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            12320..15151
                     /gene="uvrA"
                     /locus_tag="VQ846_000550"
     CDS             12320..15151
                     /gene="uvrA"
                     /locus_tag="VQ846_000550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET3075186.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDINQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            15144..16205
                     /locus_tag="VQ846_000551"
     CDS             15144..16205
                     /locus_tag="VQ846_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET3075187.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            16337..16735
                     /gene="spx"
                     /locus_tag="VQ846_000552"
     CDS             16337..16735
                     /gene="spx"
                     /locus_tag="VQ846_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET3075188.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            16801..17370
                     /locus_tag="VQ846_000553"
     CDS             16801..17370
                     /locus_tag="VQ846_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET3075189.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLAIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            17457..17723
                     /locus_tag="VQ846_000554"
     CDS             17457..17723
                     /locus_tag="VQ846_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET3075190.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            17727..18146
                     /gene="ruvX"
                     /locus_tag="VQ846_000555"
     CDS             17727..18146
                     /gene="ruvX"
                     /locus_tag="VQ846_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET3075191.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            18162..18467
                     /locus_tag="VQ846_000556"
     CDS             18162..18467
                     /locus_tag="VQ846_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET3075192.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            18773..20023
                     /locus_tag="VQ846_000557"
     CDS             18773..20023
                     /locus_tag="VQ846_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET3075193.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            20020..20562
                     /locus_tag="VQ846_000558"
     CDS             20020..20562
                     /locus_tag="VQ846_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET3075194.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            20708..22240
                     /gene="cls"
                     /locus_tag="VQ846_000559"
     CDS             20708..22240
                     /gene="cls"
                     /locus_tag="VQ846_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET3075195.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            22318..22461
                     /locus_tag="VQ846_000560"
     CDS             22318..22461
                     /locus_tag="VQ846_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075196.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            22498..24036
                     /locus_tag="VQ846_000561"
     CDS             22498..24036
                     /locus_tag="VQ846_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET3075197.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVGLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            24156..26363
                     /gene="nrdD"
                     /locus_tag="VQ846_000562"
     CDS             24156..26363
                     /gene="nrdD"
                     /locus_tag="VQ846_000562"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET3075198.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHHLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            26375..26515
                     /locus_tag="VQ846_000563"
     CDS             26375..26515
                     /locus_tag="VQ846_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075199.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            26559..27065
                     /locus_tag="VQ846_000564"
     CDS             26559..27065
                     /locus_tag="VQ846_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3075200.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            27058..27648
                     /gene="nrdG"
                     /locus_tag="VQ846_000565"
     CDS             27058..27648
                     /gene="nrdG"
                     /locus_tag="VQ846_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET3075201.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLGQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELSDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            27645..28262
                     /locus_tag="VQ846_000566"
     CDS             27645..28262
                     /locus_tag="VQ846_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET3075202.1"
                     /translation="MKKKGLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELSQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAYELESLQRDIFALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            28517..28825
                     /gene="rpsJ"
                     /locus_tag="VQ846_000567"
     CDS             28517..28825
                     /gene="rpsJ"
                     /locus_tag="VQ846_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET3075203.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            29042..29668
                     /gene="rplC"
                     /locus_tag="VQ846_000568"
     CDS             29042..29668
                     /gene="rplC"
                     /locus_tag="VQ846_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET3075204.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            29693..30316
                     /gene="rplD"
                     /locus_tag="VQ846_000569"
     CDS             29693..30316
                     /gene="rplD"
                     /locus_tag="VQ846_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET3075205.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            30316..30612
                     /locus_tag="VQ846_000570"
     CDS             30316..30612
                     /locus_tag="VQ846_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET3075206.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            30630..31463
                     /gene="rplB"
                     /locus_tag="VQ846_000571"
     CDS             30630..31463
                     /gene="rplB"
                     /locus_tag="VQ846_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET3075207.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            31567..31848
                     /gene="rpsS"
                     /locus_tag="VQ846_000572"
     CDS             31567..31848
                     /gene="rpsS"
                     /locus_tag="VQ846_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET3075208.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            31860..32204
                     /gene="rplV"
                     /locus_tag="VQ846_000573"
     CDS             31860..32204
                     /gene="rplV"
                     /locus_tag="VQ846_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET3075209.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            32217..32870
                     /gene="rpsC"
                     /locus_tag="VQ846_000574"
     CDS             32217..32870
                     /gene="rpsC"
                     /locus_tag="VQ846_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET3075210.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            32874..33287
                     /gene="rplP"
                     /locus_tag="VQ846_000575"
     CDS             32874..33287
                     /gene="rplP"
                     /locus_tag="VQ846_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET3075211.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            33297..33503
                     /locus_tag="VQ846_000576"
     CDS             33297..33503
                     /locus_tag="VQ846_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET3075212.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            33528..33788
                     /gene="rpsQ"
                     /locus_tag="VQ846_000577"
     CDS             33528..33788
                     /gene="rpsQ"
                     /locus_tag="VQ846_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET3075213.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            33814..34182
                     /gene="rplN"
                     /locus_tag="VQ846_000578"
     CDS             33814..34182
                     /gene="rplN"
                     /locus_tag="VQ846_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET3075214.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            34260..34565
                     /gene="rplX"
                     /locus_tag="VQ846_000579"
     CDS             34260..34565
                     /gene="rplX"
                     /locus_tag="VQ846_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET3075215.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            34589..35131
                     /gene="rplE"
                     /locus_tag="VQ846_000580"
     CDS             34589..35131
                     /gene="rplE"
                     /locus_tag="VQ846_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET3075216.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            35149..35418
                     /gene="rpsN"
                     /locus_tag="VQ846_000581"
     CDS             35149..35418
                     /gene="rpsN"
                     /locus_tag="VQ846_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET3075217.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            35703..36101
                     /gene="rpsH"
                     /locus_tag="VQ846_000582"
     CDS             35703..36101
                     /gene="rpsH"
                     /locus_tag="VQ846_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET3075218.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            36293..36829
                     /gene="rplF"
                     /locus_tag="VQ846_000583"
     CDS             36293..36829
                     /gene="rplF"
                     /locus_tag="VQ846_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET3075219.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            36913..37269
                     /gene="rplR"
                     /locus_tag="VQ846_000584"
     CDS             36913..37269
                     /gene="rplR"
                     /locus_tag="VQ846_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET3075220.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            37287..37781
                     /gene="rpsE"
                     /locus_tag="VQ846_000585"
     CDS             37287..37781
                     /gene="rpsE"
                     /locus_tag="VQ846_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET3075221.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            37753..37977
                     /gene="rpmD"
                     /locus_tag="VQ846_000586"
     CDS             37753..37977
                     /gene="rpmD"
                     /locus_tag="VQ846_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET3075222.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            38122..38562
                     /gene="rplO"
                     /locus_tag="VQ846_000587"
     CDS             38122..38562
                     /gene="rplO"
                     /locus_tag="VQ846_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET3075223.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            38575..39885
                     /gene="secY"
                     /locus_tag="VQ846_000588"
     CDS             38575..39885
                     /gene="secY"
                     /locus_tag="VQ846_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET3075224.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            40036..40674
                     /locus_tag="VQ846_000589"
     CDS             40036..40674
                     /locus_tag="VQ846_000589"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET3075225.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            40791..41009
                     /gene="infA"
                     /locus_tag="VQ846_000590"
     CDS             40791..41009
                     /gene="infA"
                     /locus_tag="VQ846_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET3075226.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            41034..41150
                     /gene="rpmJ"
                     /locus_tag="VQ846_000591"
     CDS             41034..41150
                     /gene="rpmJ"
                     /locus_tag="VQ846_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET3075227.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            41168..41533
                     /gene="rpsM"
                     /locus_tag="VQ846_000592"
     CDS             41168..41533
                     /gene="rpsM"
                     /locus_tag="VQ846_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET3075228.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            41551..41934
                     /gene="rpsK"
                     /locus_tag="VQ846_000593"
     CDS             41551..41934
                     /gene="rpsK"
                     /locus_tag="VQ846_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET3075229.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            41977..42912
                     /locus_tag="VQ846_000594"
     CDS             41977..42912
                     /locus_tag="VQ846_000594"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET3075230.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            42924..43310
                     /gene="rplQ"
                     /locus_tag="VQ846_000595"
     CDS             42924..43310
                     /gene="rplQ"
                     /locus_tag="VQ846_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET3075231.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            43575..43841
                     /locus_tag="VQ846_000596"
     CDS             43575..43841
                     /locus_tag="VQ846_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET3075232.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            43851..45188
                     /locus_tag="VQ846_000597"
     CDS             43851..45188
                     /locus_tag="VQ846_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET3075233.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            45432..46124
                     /locus_tag="VQ846_000598"
     CDS             45432..46124
                     /locus_tag="VQ846_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET3075234.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(46227..47872)
                     /locus_tag="VQ846_000599"
                     /pseudo
     CDS             complement(46227..47872)
                     /locus_tag="VQ846_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(47862..48485)
                     /locus_tag="VQ846_000600"
     CDS             complement(47862..48485)
                     /locus_tag="VQ846_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075235.1"
                     /translation="MDEPLSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAI
                     SDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDG
                     YALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASK
                     IQVSEESTFEEELQKGNRIRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            complement(48471..48593)
                     /locus_tag="VQ846_000601"
                     /pseudo
     CDS             complement(48471..48593)
                     /locus_tag="VQ846_000601"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(48570..48940)
                     /locus_tag="VQ846_000602"
                     /pseudo
     CDS             complement(48570..48940)
                     /locus_tag="VQ846_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289359.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(48953..49321)
                     /locus_tag="VQ846_000603"
     CDS             complement(48953..49321)
                     /locus_tag="VQ846_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET3075236.1"
                     /translation="MTVGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKSK
                     NMENAKKFIDFIISQEVQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIK
                     NKSDIVKKYNEVFTDIQSKQ"
BASE COUNT        14868 a         9324 c        10873 g        14313 t
ORIGIN      
        1 tcctgttctt atttgatttc actatagtaa ggtgcagcag gataaagaaa aaccgagaag
       61 tttctttctc ggatttgctt attatgaggt tcctattttc tatgctccaa gagttgattg
      121 atgtgatcta cttttttagc ctctctttta tagagaatag cccagatgat gagatagaca
      181 atcgcaaact cgataatgag ctggaggtag aaaatccagt ggaaagggaa ccaaccagca
      241 agagttgcta gtgggacaaa gccagccagc ataaggaaga aatgagtcag agtggcacga
      301 agcatgctcc agtcacggct gaataagcgg ttgccaaagt tgaagagcat accgatggct
      361 gcccagataa gtgtgcagta gagcaagacc agggcaccgt gaacctgatg ctgatccatc
      421 acttggccga tgagagagta gggatttagt ggtgcgtagg tatttggtgc ataaatgagt
      481 gaaaagagga tggagaggat gaggccgata agaacaccgg cagctgcatc gtgaaatact
      541 tgttttttca tagttctaat ttctccttga tggtttttag ataacggcgt gaagagtagg
      601 tgaagctttc gtttttcaag aaaatttcta ccagaccgtt tggcgtgagc ttgaggtgag
      661 agatggaatc gatattgatg atttctgatt gggaaatttg gataaaattg gttggcaaga
      721 gtttaagaac ctgatagagt cgcaaatcaa tgctgtaggt ctgactcgcg gtttctgcta
      781 gaaccttccg attctcgata tagaagcgct gaatcttgcc aatctcaact agatagacct
      841 gatcatcgat tttccctttg attttttctc tttggtccag attttctgcg aactcgatga
      901 ctttctggac tttatctgtc ggctgaggtg cttggacaat cagcttttcc tcctcgtaag
      961 tctcactaat ctgtagttct actttcatag ttttctctcc ttttcagtta tacaaggttg
     1021 tgatcacttc ctgtatatcc ttattaaaca ctattttata gcccatggca aggaaccttg
     1081 tctatgtgga gggattgggc tcctatgtgg tggagctttt ctgttctttc tgaaatatgg
     1141 tataatagca ctaattaatt tctaggaaaa tagatacaga aaggggctga aagatgtctc
     1201 atattattga attgccagag atgctggcaa accaaatcgc agctggggag gtcattgaac
     1261 gtcctgccag tgtggttaaa gagttggtag aaaatgccat tgacgcgggc tctagtcaga
     1321 ttatcattga gattgaggaa gctggtctca agaaggttca aatcacggat aacggtcatg
     1381 gaattgccca cgatgaggtg gagttggccc tgcgtcgcca tgcgaccagt aagataaaaa
     1441 atcaagcaga tctctttcgg attcggacgc ttggttttcg tggtgaagcc ttgccttcta
     1501 ttgcgtctgt tagtgtcttg actctgttaa cggcggtgga tggtgctagt catggaacca
     1561 agttagtcgc gcgtgggggt gaagttgagg aagtcatccc agcgactagt cctgtgggaa
     1621 ccaaggtttg tgtggaggat ctctttttca acacgcctgc ccgtctcaag tatatgaaga
     1681 gccagcaagc ggagttgtct catatcattg atattgtcaa ccgtctgggc ttggcccatc
     1741 ctgagatttc ttttagcttg attagtgatg gcaaggaaat gacgcggaca gcagggactg
     1801 gtcaattgcg ccaagcaatc gcagggattt acggtttggt cagtgccaag aagatgattg
     1861 aaattgagaa ctctgaccta gatttcgaaa tttcaggttt tgtgtccttg cctgagttga
     1921 ctcgggctaa ccgcaattat atcagcctct tcatcaatgg tcgttatatc aagaacttcc
     1981 tgctcaatcg tgctattttg gatggtttcg gaagcaagct tatggttgga cgttttccac
     2041 tggctgtcat tcacatccat atcgaccctt atctagcgga tgtcaatgtg catccaacta
     2101 agcaagaggt gcggatttcc aaggaaaaag aactgatgac cctggtttca gaagctattg
     2161 caaatagtct caaggaacaa accttgattc cagatgcctt ggaaaatctt gccaaatcga
     2221 ccgtgcgcaa tcgtgagaag gtggatcaaa ctattctccc actcaaagaa aatacgctct
     2281 actatgagaa aactgagccg tcaagaccta gtcaaactga agtagctgat tatcaggtag
     2341 aattgactga tgaagggcag gatttgaccc tgtttgccaa ggaaaccttg gaccgattga
     2401 ccaagccagc aaaactgcat tttgcagaga gaaagcctgc taactacgac cagctagacc
     2461 atccagagtt agatcttgct agcatcgata aggcttatga caaactggag cgagaagaag
     2521 catccagctt cccagagttg gagtttttcg gacaaatgca cgggacttat ctctttgccc
     2581 aagggcgaga tggactttac atcatagatc agcacgctgc tcaggaacgg gtcaagtacg
     2641 aggagtaccg tgaaagcatt ggcaatgttg accagagcca gcagcaactc ctagtgccct
     2701 atatctttga atttcctgcg gatgatgccc tgcgtctcaa ggaaagaatg cctctcttag
     2761 aggaagtggg cgtctttcta gcagagtacg gagaaaatca atttattcta cgtgaacatc
     2821 ctatttggat ggcagaagaa gagattgaat caggcatcta tgagatgtgc gacatgctcc
     2881 ttttgaccaa ggaagtttct atcaagaaat accgagcaga gttggctatc atgatgtctt
     2941 gcaagcgatc tatcaaggcc aatcatcgta ttgatgatca ttcagctaga caactcctct
     3001 atcagctttc tcaatgtgac aatccctata actgtcctca cggacgtcct gttttggtgc
     3061 attttaccaa gtcggatatg gaaaagatgt tccgacgtat tcaggaaaat cacaccagtc
     3121 tccgtgagtt ggggaaatat taaaagtata aaaaagtctg ggaaaaattt tcaaaatcag
     3181 aaaaacgcat aaaatcaggt gttcaaaaac cttgatttta tgcgttttat catggaaata
     3241 gttacttcat gttttcctaa ttcttttcga aactcttttt aaaccacgtc agttttatca
     3301 gtaatctcaa aacagtgttt tgagctaatt ttgccagttt tgtctgtaac atcgaagttg
     3361 tgtttgacca ctctgcgact ggtttcctag tttgctctat gattttcaca gagtattaaa
     3421 ttgcgatttt gccaagtttc tttattcgtc taaaagtaga gtctgttcta tgcgtctaat
     3481 gtacgaatta ggttgaccat ttcaatagct ccttgtgcac actcagaacc cttatttcct
     3541 gctttagtgc cagctcgttc tatggcttgt tcaattgtat ctgtcgttag cacaccaaac
     3601 ataacaggaa tttcgctatt taaactgatt tgggcgattc ccttagatac ctcgctacat
     3661 acataatcat aatgacttgt attccctcta atgacagctc ccaagcagat aattgcatca
     3721 tattttttac tttttgccat ttttgatgca atcagtggta tttcaaaagc tcctggaacc
     3781 caggctacct ctatatcttt ctcgtttaca ttctctcttt tgagattatc tagtgctcca
     3841 gataataatt ttgaagttat aaattcatta aatctcgcta caacaatacc tattttaata
     3901 ttgtttgcta ctaaattacc ttcataagtg ttcatttatt tttcctccat atttaaaatg
     3961 tgacccattc gatttttctt tgtttctaaa taaaaactat cgtaaggatt ggcttctatt
     4021 tcgattgata ttctactgga aatggtaatt ccatattttt ctaactgttc aaccttgtca
     4081 ggattatttg tcagtaaatg aagtgactga agtcccagat ctttaagcat ttgtgctcca
     4141 atatgatatt ctcttaaatc accttcaaag cctaatgcaa gattggcatc aagcgtatcc
     4201 atgccttgat cttgtaaatg ataggctttt aatttattga taagtccaat tcctcgtccc
     4261 tcctgtcgca agtaaagtaa gacacccgaa ccattctcaa caatcatttt catagcttta
     4321 tcgaattgct gtccacaatc gcaacgtaaa gagcctaaaa catctcctgt taaacattcg
     4381 gagtggaccc gacataatac attggcttca tcctctatgt ttcccataat aagagcaaga
     4441 tgatgttccc catttagttt atctatatag ctaattgctt tgaaattacc gtatctagta
     4501 ggcatattga cagttgaaac tcgttctacc agctgatcat atacttttct atattcttgt
     4561 aattctttga tggtaattag tggaatgttg tgtttcttcg agaactgaat taaatcatct
     4621 gttctcatca ttttgccatc atgattcatt atttcacaac ataggccaca ctcttttagt
     4681 ccagctaatt ttaataaatc aacagttgct tctgtgtgtc catttctttc tagaacacca
     4741 cctttttttg caattaaagg aaacatgtgt cctggcctgc gaaaatcaga gggtgttata
     4801 tcttcagcta cacacatacg tgcggtcagt cctctttcct cggcagaaat acctgtggtc
     4861 gtttctttat aatcaattga aactgtaaaa gcagtcttat gattatctgt attgttttca
     4921 accataggtg aaagcattaa ttgattagct aaactttcgc tcataggcat acaaattaat
     4981 cctttggcat aagtagccat aaaattaaca ttttctgttg tagctgcttg tgcagaacaa
     5041 attaagtctc cttcattttc tctatccttg tcgtctataa caagaacaag tcgtcccttc
     5101 tgcaatgctt ctaatgcttc ttgtattttt cgatattcca ttgactgatt atcctttctg
     5161 ctaaaatcca ttttgatata atagttcctt agatatttct gattttggag agttatccat
     5221 cagtttttgc acatatttac ctaagatatc attttcaaga tttactgtac tcccgacttg
     5281 tttactctta agaatggttt gttccaaggt atgagggata acagatactg aaaagtttac
     5341 tttggagact ttagcgacag tcagactaat gccgtcaatt gtaatagatc ctttttcaac
     5401 tattaaatct aaaatttctt tttgtgtgtt gatttgatac catacagcat tatcatcttt
     5461 ttttattgac gagatttttc ctgtaccatc aatgtgtcct gtaacgacgt gacccccaag
     5521 tcgaccgttg acagataagg ctctttctag attcacctca cttccatgtt ttaatagagt
     5581 aagagctgtt cgactccatg tttcattcat tacatcaact gtaaaggatt gatgattgaa
     5641 atgagtaact gtaagacaga taccatttac tgctatacta tcgcctaaat ggatatccgt
     5701 taatattttt gaggctttaa ttgatagttt acaattacga gagtctttct gtattctttc
     5761 aacttttccg atttcttcaa ttattcctgt gaacatggat aaatcacttc actttctatg
     5821 agatagtcat ttcctatttg agaaaatgca taaggtttca atctaatagc gtcatttggc
     5881 aaagaaatgc cttcacctcc gacaggaaac ttggcactgc ctccaaaaat ttttggtgca
     5941 atatatattt tcagctcatc aacaatttgt tgttccaaag cactccaatt cattagactg
     6001 cccccttcta gaactaggct atcaatctgc atgtttccta gatgttgcat taaactcgat
     6061 aagtctatat gattgccttt tttctttatg gaaagtattt cacagccatg attttgatat
     6121 agcttcattt tatttttgtc ttcagaggaa gtggcaatgt aagttttaat atcatttgct
     6181 gtttttacga ttttagaggt aagaggagtt cgtaaatgtg tatcgcatat gatacggata
     6241 ggatttttcc cttcctccaa tctacatgtc agcaaaggat cgtcttgaat aacagtattg
     6301 actcccacca taattgcact aacatggtgt cgtaactgat gcacatgctt tcttgcttct
     6361 tcttcagtaa tccatttgga ttgatttgtt ttagtggcta tttttccatc cattgacatt
     6421 gcatatttca taaaaacata gggtacatgc tgggtaatat actttctaaa actttttatt
     6481 aagttaagac actcattttc taaaattcca acagtaactt gaagattatt ttcctcaagt
     6541 atctttactc cttttccaga tacaatagga ttacagtcta ggcttccaat gactactctt
     6601 gtaataccac tatcgattat agcatctata cagggaggtg ttttcccgaa gtgacaacag
     6661 ggttcaagtg ttacataaag cgtcgctccg acaggggatt ttctacagtt tttaagagca
     6721 tttctctcag catgtgggcc accaaaaaac tcatgataac cttgtccgat aatgtgatta
     6781 tcttttacaa taattgcgcc aaccatagga ttgggattga cgtaaccagc ccctttttgt
     6841 gccagtttta ttgctaattt catatatttt gaatcgctca tctcactacc tccaaaaaaa
     6901 tataccttga ataggggact actcaaggca tacaaaagaa aacttatgcg attaacaaaa
     6961 atgctctgaa atgacaagta atcatttcag agcacgcaaa aagcacaaat atacttttat
     7021 cttctttcat ccagactata ctgtcggctt tggaatttca ccaaatcatg cctttcggct
     7081 cgtgggctat accaccggta gggaattgca ccctgccctg aagatagtta ttcaattaca
     7141 gatgattata gtacttaatt ttgaatatgt caacagataa atacagattg tttttgatat
     7201 actgtatttg tgataatcga ttctcgctcc tcggataaag aaaatatgat atactagata
     7261 aacgaaataa gagagaagga acactatgta cgcatattta aaaggaatca ttaccaaaat
     7321 tactgccaaa tacattgttc ttgaaaccaa tggtattggt tatatcctgc atgtggccaa
     7381 tccttatgcc tattcaggtc aggttaatca ggaggatcag atttatgtgc atcaggttgt
     7441 gcgtgaggac gcccatttgc tttatggatt tcgctcagag gatgagaaaa agctctttct
     7501 tagtctgatt tcggtctctg ggattggtcc tgtatcagct cttgctatta tcgctgctga
     7561 tgacaatgct ggcttggttc aagccattga aaccaagaac atcacctact tgaccaagtt
     7621 ccctaaaatt ggcaagaaaa cagcccagca gatggtgctg gacttggaag gcaaggtagt
     7681 agttgcagga gatgaccttc ctgccaaggt cgcagtacaa gcaagtgctg aaaaccaaga
     7741 attggaagaa gctatggaag ccatgttggc tctgggctac aaggcaacag agctcaagaa
     7801 aatcaagaaa ttctttgaag gaacgacaga tacagctgag aactatatca agtcggccct
     7861 taaaatgttg gtcaaatagg agcagagaat gacaaaacgt tgttcgtggg tcaagatgac
     7921 caacccgctc tacatcgcct atcatgatga ggagtggggt cagcccctcc atgatgacca
     7981 agcattgttt gagttgttgt gtatggaaac ctatcaggca ggcctgtctt gggaaacggt
     8041 actcaacaaa cgccaagctt tccgagaagc ctttcatagc tatcaaattc actcagtcgc
     8101 agagatgact gacactgaat tggaagccat gctggagaat ccagctatca ttcgaaatag
     8161 agccaagatt tttgctacac gcgctaacgc ccaagccttt ctacagttac aggcagagta
     8221 cggctctttt gatgcctatc tttggtcttt tgttgagggg aaaactgtcg ttaacgatgt
     8281 tcctgattat cgccaagccc cagctaaaac acccttatct gagaaattag ccaaagatct
     8341 caaaaaacga ggcttcaagt tcacaggccc agtcgccgta ttgtcttttc tacaggctgc
     8401 agggctagtt gatgaccacg agaatgattg tgagtggaaa ggtcttaaat gatgtctaac
     8461 aaaaataagg gaattctgat ttttgcgatt ctctatacaa tcctctttgt gtttgatggc
     8521 gttaaattgc tggcttcttt aatgccatct gtcattgcaa attatcttgt ttatgtagtt
     8581 ttagctttat atggctcctt cttgttcaag gatagattga tccaacaatg gaaggagatt
     8641 agaaagacta aaagaaaatt cttctttggc gtcttaacag gatggctctt tctcattctg
     8701 atgactgttg tctttgaatt tgtatcagag atgttgaagc agtttgtggg attagatgga
     8761 caaggtctaa atcagtctaa tattcaaagt acctttcaag aacaaccact actgatagct
     8821 gtttttgctt gtgtcattgg acctctggta gaagaattat ttttccgtca ggtcttattg
     8881 cattacttgc aggaacggtt gccaggttta ctaagcatta ttctggtagg acttgttttt
     8941 gctctgactc atatgcacag tttggctcta tcagagtgga ttggtgcagt tggttactta
     9001 ggtggaggcc ttgccttttc tattatttat gtgaaagaaa aagagaatat ctactatccc
     9061 ctacttgttc acatgttaag caacagcctc tccttaatca ttttagctat cagtatagta
     9121 aaatgaaatg agaacaggac aaatcgattt ctaacaatgt tttagaagta gaggtgtact
     9181 attctagttt caatatactg taatatgtga tgaaaatgcc agtaatgata ccgagaaaaa
     9241 agctgagaaa cttttcccag ctttatttgt tatagtcaaa gagaatgact tgttcctgtg
     9301 catctacatg agcatggacc ccaaagggta caattgctct tggagttgcg tggccgacat
     9361 tcagattata gacaatcggg atattgctgt caatgatatc caatagtgcc tctttatagt
     9421 cgtcatggaa agtttcatcc ataggttttc cgaccaagag tccattgatg accgcgaata
     9481 tgccagtgtc ctttaaagtt agcaacattt ttttgaagtc ttctggctta ggcttttctt
     9541 cgcttgtttc gagcaagagg attttccctt cccagtctga caagtcaggg aaaagtttgt
     9601 atttttggca gagttccgtg ctatctgcgt atcgagagtt gtcaaagata tcgtagaggg
     9661 attcgaggca accaccgagg attttcccct cgaactgagc acttccttgc aacaagtcaa
     9721 aacctgtatt tgtatgactg acacgaggtg ttcccagggc cgtgggacta aaatcagttc
     9781 gttcctcata ccaaacgtca ctagggcgga tttctgaaat tcttcccgtc ttaatcaatt
     9841 ctttaaagta gtgaaggcta taggctagca tttctttgtc taattcacaa atgtctgcta
     9901 aaaaggattg accataaaaa gtcttgattc ctaatttatg caacatgagg tggttcatgg
     9961 ttgtatccga gaagccaaga aaaatttttt gcttgataac cttttggagt tggtcatttt
    10021 caaaaagata aggtagcaag cgataggtat cgtctccacc gatggcacat aggatcatgt
    10081 cgatgctatc atcagaaaag gcatgaatca aatcctctgc acgagcttca ggatggtcct
    10141 tgataaagtc taatcctttt aacgaatggg acaaaaagat gggattgagt cccagatcct
    10201 tgagacgttg gatacccaag tccacttcgt gtttgacaaa atcctctccg ataatgccac
    10261 tagataaact aacaatacca atagtagaaa ccatatctca tcctcctaga aatagattga
    10321 gcctatttta tcacaaaata taagagaaag ctatgtggga tttcagaaga atacttttaa
    10381 tactcttcga aaatctcttc aaaccacgtc agttttatct gcaacctcaa agctgtgctt
    10441 tgagcaacct gcagctagct tcctagtttg ctctttgatt ttcattgaat ataactcaaa
    10501 aaagtaagtg aaaaagcaac cgcacctgca gttgctttta tcgttttctt attcttgata
    10561 gcgtgttggt cctgcacttg cactacggat attcaagcgt ttcttgagat agaagcgaag
    10621 ggctaggagg gctgctccaa taatagctag tggcaatgga tccagtactg ggttaaggct
    10681 agctggtagg aagcttgttg caaagaagac aagcaaccaa aggaacatag aagctaggat
    10741 aactagtaca gatttccaga aaggtggacg ttgactgcga tccatatctg atccatagta
    10801 ttggtaaaca aagtagtaca tcaagtagaa ggcaaatcca ccaaccagtc caactaatag
    10861 aagagtaatc aatccatagc cgaaagcttg gtctgccgca aagaaggttg tgagggcgct
    10921 gacaagggca aagaggctag tgatgaaaag agctgagtcc ataatcatca gttttgggtc
    10981 atcattttct tttggatgct ctttttcgta ctgctctttg acagtgaagc tatgagccca
    11041 atgagttggt gcgccgtata gggaacgggc agttgtacct ttagattgct cctcaaggat
    11101 ttggggaatg acttcctcaa aaatagtctg gatttcagcg tctgttttcc catctttgat
    11161 gaattgttgg gtagcaatgt ggacaaactc ttggtttttc ttagttaatt tttgtagatc
    11221 aatttgagac ataggaactc ctcttagaac cattttttat ggatgagata gagagtgagc
    11281 gagacactca tagcaaaggc gataaagacg attaaccaga aggcatttgg ctctccgttt
    11341 agggggattt cattatcctt aaagttcatc ccgtaggcag aaaagaccat ggttgggatg
    11401 gacatgacga tggtcacaag ggccaaggtt ttcatgatgt tgttctggtt gttagaaatg
    11461 atagaggcaa aggtctctgt catagaatgc aagacgtttc cataaatatc tgccatctcg
    11521 atggcctgtt gggtttcaat cagggtgtct tcaagcaggt cttcgtcctc aaggtatttc
    11581 ttgatattgc tggttgaact ggtcaatttc ttaatcacgc gctcatttgt tttgagggag
    11641 gccttgaaat agacgatagt tttttccaat tccatgagct caatcaattc ttcattacga
    11701 gttgattgat gcagttgact ttcgatttgt tcactcttgc ggtcgattga acgaagggct
    11761 gttaggtaaa gctctgcatt gcgataaaga atttgaaaga taaaacgtga acgcatgaag
    11821 gtatagaaat tacgcaatcg acggttgata aagacatcaa ggacaggtag tggttccaaa
    11881 cacgtagtga taatggtttc ctcagtgatg ataataccaa gcgggatggt tacgtagtag
    11941 gtgcggttat ttctttcctc cgtgaccggc acgtctacga taatcagggt atactcgtct
    12001 tcaatggtaa tacgagacat ttcttccgca tcgagcggtg ctcgaaggtc ggcaatatca
    12061 atatcgaagg tgttggcgat ttcgagtgat tcattttgag tcggattgac gagattgatc
    12121 caagtacccg gttcaagcgt atcgatctct ttaaattcag ttgttgtaga gagaaaaact
    12181 tgtttcatat cccttatcct ttctcatttt tcagattttt tcacactgta ctattatact
    12241 acaaaatcgg ccttttgggt aatagaatct cacttttttt ggtataatgg taagcaataa
    12301 tggactagaa agaacaaaga tgcaagataa aattgtcatt catggggcgc gtgcccataa
    12361 tttaaaaaat attgatgtgg agattccgcg agacaagttg gttgtcgtga ctggcttgtc
    12421 aggttcaggg aagtccagtc tggcttttga tactctctat gcggagggac aacgtcgcta
    12481 tgtagagagt ttgtccgcct acgctcgtca gttcttggga aatatggaaa aaccagatgt
    12541 agatgccatt gatggtctca gcccagctat ttccatcgac cagaaaacga ctagtaaaaa
    12601 tcctcgctcg acggtgggaa cgacgactga aatcaatgac tatctgcgtc tcctctacgc
    12661 acgtgtgggg acgccttact gtatcaacgg acatggagct atcaatgctt cttctgtgga
    12721 gcaaatcgtt gataaggttt tggagttacc tgaacgccag cgcttgcaga tcttggctcc
    12781 tgtcatccgc aagaaaaaag gccaacataa gagtgttatc gagaaggttc agaaagacgg
    12841 gtatgttcgt gtccgtgtgg atggggaagt ctatgatgtg accgaagtgc cagagttgtc
    12901 taagagcaag caacacaata tcgatgtcgt ggttgaccgt attgttatca aggagggcat
    12961 tcgtagtcgt ctctttgatt ccattgaggc tgcccttcgt attgcagaag gttatgtcat
    13021 tatcgacacc atggacgact cggagttgct cttctctgag cattatgcct gtccagtttg
    13081 tggatttact gtcccagagt tagaacctcg tctcttctct ttcaatgctc cctttggctc
    13141 ttgtagtgag tgtgacggct tggggattaa gctggaggtg gatactgatt tggtagtgcc
    13201 agatgccagc aaaaccttac atgagggagc gctggcacct tggaatccta tctcatccaa
    13261 ctactatcca aacatgttag agcaggccat gaaagtcttt ggagtggcta tggataagcc
    13321 ttttgaggac ctgtcagaag aagataagaa cttgattctc tacggctcag atggtaagga
    13381 attccatttc cattatgaaa atgaatttgg tggtgtgcgc gatatcgaca ttccgtttga
    13441 gggagttatc aataatatca aacgtcgtta ccatgaaacc aatagcgatt acactcgcac
    13501 tcagatgcgt ctctacatga atgagctgac ctgcggaacc tgtcaaggct atcgtctcaa
    13561 tgaccaggcc ttgtctgtcc gtgtgggcgg ccagcaaggg ccacatattg gagaaatctc
    13621 agacctgtct atcgctgacc acttggactt ggtgagccag ttaactttgt ctgaaaatga
    13681 agccatcatt gctcgtccca ttctcaagga aatcaaggat cgtttgacct tccttaataa
    13741 tgtgggtctt aactatctaa ccctgtcacg ttcagcagga accctttcag gtggggaaag
    13801 tcagcgcatt cgtttggcaa cccagattgg ttccaaccta tcaggtgtcc tctatatcct
    13861 agacgagccg tcaatcggtc ttcaccagag ggacaatgac cgcctgattg ccagtctgaa
    13921 aaagatgcgt gacttgggca atactcttat cgtggtggaa cacgacgaag ataccatgcg
    13981 tgaagctgat tatctgattg acgttggtcc tggtgccggt gtttttggtg gtgagattgt
    14041 tgcagcaggt acacctaagc aagtggctcg caacagcaag tctatcacag gtcagtactt
    14101 gtcaggcaaa cgtgtcatcc cagtaccaga agagcgccgt gtcggaaatg gtcgttttat
    14161 tgaagtgaca ggagcgcgtg agaacaactt gcaaaatgtc acggctcgct ttccactagg
    14221 aaaatttatc gcagtgacag gtgtgtcggg ttcagggaaa tcgactctaa tcaacagcat
    14281 tctcaaaaaa gccattgccc agaagctcaa ccgtaattca gacaaacctg gtaagtttaa
    14341 gactattaca gggattgagc atgtcgaccg tttgattgat attaaccaga gccctatcgg
    14401 acggacgccg aggtctaacc cagctaccta tacaggagtt tttgacgata tacgtgacct
    14461 ttttgctcag acaaatgaag ccaagattcg aggctacaag aaggggcgct tcagtttcaa
    14521 cgtcaaggga ggtcgctgtg aagcttgctc aggtgacggg attatcaaga ttgagatgca
    14581 cttcttgcca gatgtttatg tggcttgtga agtttgccac ggaacccgct ataacagtga
    14641 aaccctagaa gttcactaca aggaaaagaa tatttcgcag gtcttggata tgacggtcaa
    14701 cgatgcggta gaatttttcc aacacattcc gaaaattcaa cgcaaactcc agaccatcaa
    14761 ggatgtaggt ctaggctatg tgaccttggg acagccagct accacccttt ctgggggaga
    14821 agcccaacgt atgaagttag ctagcgaact ccacaaacgc tcgacaggaa aatctttcta
    14881 cattctggat gagccgacga cagggcttca tacagaggac attgctcgcc tgctcaaggt
    14941 tttagctcgc tttgtagacg ccggcaatac agtcctcgtc atcgagcaca atctggatgt
    15001 tatcaagacg gcagaccata tcattgactt gggacctgag ggcggtgtcg gtggtggaac
    15061 catcatcgta acaggaactc cagaagaagt agctgccaac gaagccagct atacaggaca
    15121 ctatttgaaa ggaaagttac atcatgaata aacgcgtaca agcatttcta gctaaaatgc
    15181 aagaaaaaga actagatggt atcatcatca acaatcttaa aaacgtctat tatttgactg
    15241 gtttttgggg ctcaaacgga acagtcttta tcagccgtga ccgtcaggtc ttagtgacag
    15301 actctcgcta tatcatcgca gctaagcaag aaaccagtgg ttttgagatt gtggctgatc
    15361 gtgatgaatt ggctgtcatt gcaggaattg ttaaggacat gaacttgact cgtatcggtt
    15421 ttgaagatga gatttcagtg tcttattacc accgtatgca ggcagctttt gcaggtttga
    15481 acttgcttcc acaaactcag tttgtggaag gtcttcgtat gattaaggat gaggcagaga
    15541 ttgcagcgat tcgcaaggct tgttctatct cagaccaagc tttccgcgat gcgcttgact
    15601 ttatcaaacc aggaaaaact gaaattgaga ttgccaactt ccttgatttc cgcatgcgtg
    15661 agttgggagc atctggctta tcttttgata cgatcctagc tagcggtatc aattcttcta
    15721 aaccccatgc ccatccaatg cacaaaccag tggagttggg agaagccatc accatggact
    15781 tcggctgtct ctatgaccac tatgtcagtg atatgacccg gactatctat ctagggcatg
    15841 ttagcgatga gcaggcagag atttacaata cggttctaaa agctaaccaa gccttgattg
    15901 accaagctaa ggcaggctta ggtttccgtg actttgacaa aatccctcgt gatattatca
    15961 ttgaggcagg ttatggtgac tactttactc acggcattgg ccacggtatt ggtctggata
    16021 tccatgagga accctacttt agtcagactt ctacagaaac tattaagaca ggtatggcct
    16081 tgaccgatga accaggtatc tatatcgaag gcaaatatgg cgttcgtatc gaggatgata
    16141 tcctgattac agagacaggt tgtgaattat tgaccctagc tccaaaagag ttgatagtca
    16201 tttagttgtt tgtcaagaaa aaagtacgaa aatgacgaaa aaagtcaaaa aaaattaaaa
    16261 ataggtcgca agtcggatgt tttttatggt ataatagact aaactgatag taacatgtag
    16321 cgaaaggggt aggtacatga ttaaaattta tacagtctca agttgtacta gctgtaaaaa
    16381 agcaaaaacc tggctcaatg cccaccagtt aagttataaa gaacaaaacc ttggtaaaga
    16441 aggaattacg agagaagaat tactggatat tctaaccaaa acagataacg gaatagccag
    16501 catcgtttcg tctaaaaatc gctatgccaa agcccttgga gtggatattg aagatttgag
    16561 tgtcaatgaa gttctcaatc tgattatgga aacaccgaga attttaaaga gcccaatcct
    16621 tgtagatgaa aaacgcctgc aagttggcta caaggaagac gatattcgtg ccttcctacc
    16681 acgatctgtc cgtaatgtag aaaatgcaga agcacgtttg cgtgcagctc tataaacatc
    16741 aaggctggga gcacttccca gtcttattct attttaattt caaaaagaaa gaagaaagaa
    16801 atgaaaaaaa tagttcttgt tagtctagct ttcctttttg tcctggttgg ttgcggacag
    16861 aaaaaagaaa ctggaccagc tacaaaaaca gaaaaagata cgcttcagtc ggcattgcca
    16921 gttattgaaa atgctgagaa gaatacagtt gtaactaaga ctttggtctt gcccaagtca
    16981 gatgatggta gccagcagac acaaacaatt acttacaaag acaagacttt tttgagtcta
    17041 gctatccaac aaaaacgtcc agtctctgat gagttgaaga cttatattga ccaacatgga
    17101 gtggaggaaa ctcaaaaagc tcttcttgaa gcggaggaga aggataagtc tatcattgaa
    17161 gctcgtaaat tggcaggttt caaacttgaa acaaaactat tgagcgcaac ggaacttcaa
    17221 acaacgacta gttttgattt tcaagttctg gatgtcaaga aggcttccca gttggaacat
    17281 ctgaagaata ttggtttgga aaatcttttg aaaaatgaac caagcaaata tatttcagat
    17341 agattggcaa atggcgcgac agaacaatag aaaatccaaa taaatagact gcctgaattg
    17401 tagaaggtaa ggaattatgc tataatggta ctattctaag gaaagagggt gttagaatgg
    17461 gatttactga agaaacagta cgttttaaat tggacgattc caataaaaaa gaaattagcg
    17521 aaactttgac agatgtttat gcttcgttga acgataaggg ttacaaccca attaaccaaa
    17581 tcgtaggtta cgtattgagt ggagaccctg cctacgttcc tcgttataat aatgcacgaa
    17641 atcaaatccg taagtatgag cgtgatgaaa tcgttgagga attggttcgc tactacctca
    17701 aaggacaagg agtcgatcta taacctatga gaattatggg attggacgtc ggttcaaaaa
    17761 cggtaggggt ggcgattagc gatccgcttg gttttacagc tcaagggctt gaaatcatcc
    17821 agataaatga agaacaaggc caatttggtt ttgaccgcgt taaggaattg gttgatactt
    17881 acaaggtgga acgatttgta gtgggcttgc ctaaaaacat gaacaataca agtggaccgc
    17941 gcgtagaagc tagtcaagcc tacggagcaa agctagaaga gttttttggt ttaccagtag
    18001 actatcagga tgaacgcttg acaacagtgg ctgctgagcg catgttgatt gaacaagcag
    18061 atatcagtcg caataagcgc aagaaagtca ttgataagtt agcagctcag ctgattttac
    18121 aaaattattt agatagaaaa ttttaatata aaggagaggc tatgtcacac gatcataacc
    18181 acgaccacga agaacgtgaa ctaatcacac tagtagatga gcaaggaaat gaaaccttgt
    18241 ttgaaatcct tttgacaatt gatggaaaag aagaatttgg taaaaactat gttcttctag
    18301 taccagttaa cgcagaagaa gacgaagacg gacaagttga aatccaagct tactcattca
    18361 ttgaaaatga agatggaaca gaaggcgaat tgcaaccaat cccagaagac tcagaagacg
    18421 aatggaacat gattgaagaa gtcttcaaca gctttatgga ggagtaaaaa cgtccggggg
    18481 acgtttttag ccctgcgcta gaaattagaa acgcaggtag tctgggagac tgtatcagcc
    18541 caactccaga aatggggaag agttggaacg tccagtgaac atttttaccc caactcttag
    18601 aaattgagaa gagttggaac atctagtgga tgtttttagc ccatattcac attcatagta
    18661 gctagttatt agctaaccag gtaagaggtc gggacgaaaa tcctggcctt gtttttatta
    18721 gtaaccatac tttaatgcga taattgattg gacttttgtc aaggagatgt gtatgtttga
    18781 agtagaagaa tggctccata gtcggattgg tttgaatttt cgatcaggtt tgggccgaat
    18841 gcagcaagcg gtggatttgt taggaaatcc tgagcagtct taccctatta tccacgtaac
    18901 agggactaat gggaaaggat ctaccattgc ttttatgagg gaattattta tggggcatgg
    18961 caaaaaagtt gcgaccttta cctcccctca tatcgtatcc atcaatgacc gaatctgcat
    19021 taatgggcaa cctatagcag acgcagactt tatccgtttg gctgatcagg taaaggagat
    19081 ggagaaaacg cttctgcaaa ctcctgacca gttgtccttt tttgaattgc tgaccttggt
    19141 tgcttttctt tattttaggg agcaggaggt ggatttggtt ttattagaag tgggaattgg
    19201 tggcttactt gacacgacca atgtggtaac tggagagctt gctgtcatca cctccattgg
    19261 gcttgaccat caagaaacct tgggtgatag tctagaagca attgcagagc agaaagctgg
    19321 tattttcaag gctggtaaaa aggcagtgat tgcgaaattg cctccagaag ctagacttgt
    19381 ctgtcagaaa aaagccgaat ctttagctgt tgacctttat caggcaggtc aagatttttc
    19441 agtgctgaat ggtgattttt caagctcttt actaaatctt tcacagctga acataggctt
    19501 agaaggagcc tatcagcagg agaatgcggc cttggcgttg caaacttttc ttctttttat
    19561 gggagaaaga aaggaagcta ttgatgaaca ggctgtaaga aaggccttgg aacagaccca
    19621 ttgggctggt cgcttggagc gtattcgccc acagatttac ttggatggtg ctcataacct
    19681 ccctgccttg actcgcttgg ttgagtttat caaagaaaaa gagcaggaag gctatcgacc
    19741 tcaaatcctc tttggatcct tgaaacgtaa ggattatcaa gggatgttgg gttatctgac
    19801 tgaaaattta cctcaagtgg aactcaaaat gaccggcttt gactatcaag ggtctttgga
    19861 cgagacagat gtaacaggtt accatgtaat tccctcttac cgagaattta tcagcagttt
    19921 cgaagaaagg gcagacgctc aagacttgct gttcgttaca gggtctctct attttatctc
    19981 agaagtacgg ggctacctgc tggaccgtga gcagataaat tgaccccctt ttttgaactt
    20041 gttatactag gggaattgct ggttagagaa aatttctcta aattggtagc agaaaggaaa
    20101 ttcatcatga aattaaaaag attcacactt tctcttgctt ctctagcaag ttttagtctc
    20161 ttagtagctt gttcacaaag agctcaacag gttcaacagc ctgttgctca gcaggtccaa
    20221 cagcctgctc aacagaatac caatactgca aatgcaggag gtaaccaaaa tcaagcggct
    20281 ccagtacaaa accaacctgt tgctcaaccg accgatattg atgggactta tactggtcag
    20341 gatgacggag accgtatcac tttagtggta actggaacga ctggtacatg gactgagctc
    20401 gaatctgacg gggatcagaa agtcaaacag gttacatttg attcagcaaa tcaacgcatg
    20461 attattggcg atgatgtcaa aatttacact gtaaacggta atcaaatcgt cgtagatgat
    20521 atggatagag acccatcgga ccaaatcgtt ttaactaaat aagactatgc aagtagaaag
    20581 ggctggattt ttcagccttt ttacttttac ctagaaaata atcataaata cttgccttct
    20641 accgaatacc tgcgttatac tagtatcaat tacttgtttt tagcattcaa actatcaagg
    20701 aggggatatg aaatatagga aatttcaatt attgatgtcc aagtatggct ttagtctttc
    20761 gattatgcta cttgaacttt gtcttgtttt tggtctcttt ctttatttag gacgcatggc
    20821 tcccatttta tggattactg tcctcattct actgagtatc atcacaatca tttcgatagt
    20881 caaccgtaat acgactcctg agaataaggt aacctggttg ttagtagcct ttgtgccagt
    20941 atttggtccc ttgctctatc tgatgtttgg tgaaaggcga ttgtccaaaa aagaaatcaa
    21001 acaactgaag aagctaggct ctatgcattt ccaagaagca aatagccagc tactaaaaga
    21061 gaaattaaaa gaaagtgaca aggcagctta tggagtcatc aagtccttat tgagtatgga
    21121 taccaatgct gacatctatg atcaaactgc ctctacattt tttcctaacg gagaagctat
    21181 gtggaaaaag atggtagaag atcttaaaaa ggctgagaaa tttattttct tggaatatta
    21241 cattatagaa gaaggtttga tgtggaatcg catactagat atactagagc aaaaggtagc
    21301 tcagggtgta gaggttaaga tgctctatga tgatatcggc tgtatggcta ctttaacagg
    21361 agattatgca catcgacttc gtcagctggg catcgaggcc cataaattca ataaagttat
    21421 tcctcgtttg acagtggctt ataataacag agatcataga aaaatattga ttgttgatgg
    21481 tcagatagcc tatactggtg gggtcaatct ggcagatgag tacattaacc acgtcgagag
    21541 atttggttat tggaaggata gtggaattcg cttagacgga ctagcagtaa aagccctgac
    21601 acgcttattt ttgaccactt ggtacattaa tcgaggagaa attagtgatt ttgatcaata
    21661 tcatttagaa aatcattcta tcccgagtga cggtttaacc attccatacg gaagtggacc
    21721 caagccaatt tttcgagcgc aggtagggaa aaaagtttat cagagtttaa tcaatcaagc
    21781 aacagaatcg gtctatatta cgacacctta tttgattata gattatgatt taacagagac
    21841 aatcaaaaat gcagctatga gaggggtcga tgttcgaatt atcacccctt acataccaga
    21901 taagaagttc attcagttag tcacgagagg agcttatccc gaccttcttt ctgctggtgt
    21961 tcggatttat gagtatagtc caggttttat tcatagtaag cagatgttgg tagacgaaga
    22021 ttttgcggtg gtggggacaa tcaatctcga ctaccggagc ttggtacacc attatgaaaa
    22081 tgcagtctta ctctataaaa ctccttctat aagggaaatc gcccgagatt ttcgaaatat
    22141 atttgcagat tctcaggaag tctatcctca ttctatcaaa acgagctggt atcaaaagct
    22201 tgtaaaagaa atcgcccagc tattcgcccc tatcttataa gaaatctaga acttaggata
    22261 caaaccagcc ctatttttct tgtcttttac taatagaaag aaagaggagg ggaattcatg
    22321 ctaactaagg aagaagtgaa tgatatgatt gagttcaaac agacacattt acaccgcttt
    22381 cgggaaattg aaacctttgt gaaactatgc aaaaaatcac tcaaacagcc atcgcaagct
    22441 gacaatccta ggactttttg atatactaag acagataaat tgaacaggag aaacgatatg
    22501 agtgtgtatg gtagagtaga agaagttcat aaggagaatc gtgaacctct agaataccaa
    22561 atcgaacaag aatcgcatca tcgtgaatca agtcgtcttc ctttggtgaa aattttacta
    22621 tggagtacgc ttgtaacggg gataactcta ggagtaccgc tattactcga cttaatgagt
    22681 gcacaagaag tgcaggattt ttatgcaggt tgggcgcttc atcagacagg gaagatttac
    22741 agcgactatt atggaagtca aggtttgctt tattatttgc tgacttacgt gagtcagggc
    22801 ggatttttct ttgccatctt tgagtggtta gccttggtag caggaggatt tttccttttt
    22861 agatcagcgg acaccttgac agagcaagga gaccaagctg gacatctggt gactattttt
    22921 tacatgctag ttacaggtct tgcttttggt ggaggctatg cgactctttt agcgcttcct
    22981 ttcttattcg cagcctttag tttagttgcg gcttacctaa gcaatccaag ccatgataag
    23041 ggatttgtac ggattgggct agctttggca ggcggatttt tctttgctcc cttatcatcg
    23101 ctcctgttta ttgctgtagt gggtttaggc ttgttggtct ttaaccttgg gcatagacgc
    23161 tttgcgcatg ggttttatca gtttcttgca gtggctttag gtttttcact tgtcttttat
    23221 ccaactgcct actatagtgc tgcaacagga agttttgggg atgcgattag tggtattcgt
    23281 tatcctattg acagtattcg ctttgatttt acttctaaaa ttttagagaa tatgtttttt
    23341 tatggcttgt tgtcccttgg tttgggattt gtggtttgta tctttttagg tctctttcaa
    23401 tccaaaccct ttaaactata cgtcatttcg gttcctgcaa gtcttgttgt aattttaggt
    23461 ttgattttac ttttcttttc acaagagcct ctgcacgctt cttatttgat ggtcgtcttc
    23521 cctgttttcc tacttttatt ggtaaccaat attaagagtc aacagagggg gcgtagtgct
    23581 agaagaagcc gaagagaaac gccagttagc ctatggagtc gtttcttcaa aggaaatcta
    23641 tatctgctag tttttgggtt tgtctatctt ttgtctgttc cttttttgat gaagtttgtc
    23701 ctttatccag taccttatca agaacgtaat cgtcttgctg atttggtaaa agaggagaca
    23761 aatacggaag atgctatcta tgcatgggat gatactgcga ctctttatcg taagagtgag
    23821 cgcttgtcgc catcggcgat tttgtccccg ttgcactata cagcaactga ggaaaatcgt
    23881 aataagttac ttaatgactt gaaagaaaaa caacctaagg tgattgtggt aaatgacaag
    23941 gtggtagtct ggtctgaagt ggaaacactt ttaaaagaaa attaccaaca agtaaagact
    24001 gattactcag agtttaaagt ctataaaatt aaataaccaa atcaatatct tgtgtatttt
    24061 tagaaatttt aggattttta acacaagata ttgatttttc tttttagagt ggtataatac
    24121 tttttagaaa gaacatttta gaaaagagca tgcgtatgat tgcactagaa gaaaaaatta
    24181 caattttgcc aactctcttc gtcgagaaac gagatgggag acgtgttgta tttgatgtgg
    24241 acaagattga caaggctctc cacaaggcgg ctgacaaggt tatggatgtg acacccctgg
    24301 ttgaaaaacg cctcaatgat ctgactgagc gaattattac agaaattcat agtcgctttc
    24361 cacagggaat taagatttac gaaattcaaa atatcgtaga acatgaactc cttgaagcca
    24421 aagaatatgc gctggctgag gagtatatta cttatcggac acagagggat tttgagcgct
    24481 caaaagcgac ggatatcaac tttagtattc ataaacttct caacaaagac cagacagttg
    24541 tcaatgaaaa tgctaataaa gacagtgatg tctttaacac tcagcgtgat ttgacagcag
    24601 ggattgttgg gaaatcaatc ggactgcaaa tgcttcctaa gcacgtagcc aatgcccacc
    24661 aaaaggggga tatccactat cacgatttgg actacagtcc ctatacccct atgaccaact
    24721 gctgtttgat tgattttaag ggtatgttgg aaaatggttt taagattgga aatgcagagg
    24781 tagagagtcc caagtctatc cagactgcga cagcacagat ttctcaaatc attgccaacg
    24841 ttgcttctag ccagtacggt ggctgttcag ctgaccgtat cgatgaaatt ttggcgcctt
    24901 atgcagagaa gaactatcaa aaacatctca aagatgcaga agagtgggta ttgcctgaaa
    24961 aacgggaaga ttacgcttgg aagaaaacgc aaaaggacat ctacgatgcc atgcaatctc
    25021 ttgagtatga aatcaatact ctcttcactt caaatggaca aacacctttt acttcgttag
    25081 gttttggtct gggaaccagt cgttttgaac gagaaattca aaaagctatt ttaaacattc
    25141 gcatcaaggg tcttggttca gaacaccgta cggctatctt tcctaaactt attttcactc
    25201 tgaaaagagg ccttaattta gaggaaggaa ctcccaacta cgacatcaag cagttggctc
    25261 ttgagtgtgc aaccaaacgg atgtatccag atgttttatc ttacgataag attgttgact
    25321 tgacaggatc cttcaaggtg cctatgggtt gccgttcttt cctccaagga tggaaggatg
    25381 aaaatggtgt agaagtcaat tcaggtcgca tgaatctggg tgttgtgacg gttaatctgc
    25441 ctcgtattgc tcttgagtct gaaggtgata tgaataagtt ctgggaaatc ttcaacgagc
    25501 gcatgaatat cgcagaagat gctcttgttt accgtgtcga acgcactaaa gaggcgacac
    25561 cagcgaatgc tcctatcctt tatcagtacg gtgcttttgg ccatcatcta ggtaaagaag
    25621 aaagtgttga ccagctcttt aagaatcgtc gtgcgaccgt ttcgctgggc tatatcggct
    25681 tgtatgaagt agcgacagtt ttctttggta acagctggga aagtaatcca gatgctaagg
    25741 aattcacgct agacatcatt cacgatatga aacgccgtgt agaagagtgg tcagaccaat
    25801 atggctacca tttctctatc tactcaacac catccgaaag tctgacagac cgtttctgcc
    25861 gactagatat agacaagttt ggctctattc ctgatatcac agacaaggaa tactacacca
    25921 actctttcca ctacgatgtt cgtaaaaatc caacaccgtt tgaaaaattg gactttgaga
    25981 aagtctatcc agaagcaggt gcgtcaggtg gtttcatcca ttattgtgag tatccagtcc
    26041 ttcagcaaaa tccaaaggcc ttggaagctg tctgggatta tgcttatgac cgtgtaggct
    26101 atctaggcac caatactccg attgaccgtt gctacaagtg tgactttgaa ggggattttg
    26161 aaccaactga gagagggttt gcttgtccaa actgtggcaa tagcgaccct aaaacagtag
    26221 atgtggtgaa gcgaacttgt ggctacctag gtaatcctca agcaagaccg atggtcaacg
    26281 ggcgtcacaa ggaaatcgct gcgcgtgtca aacatatgaa tggttcaacg attaaaatag
    26341 ctgggcatca agtaacaaat tagaaagaaa tgaaatggga aaatatcaac tagacgataa
    26401 ggggcgcgca caagtgaccc gttatcacga gaaacactct aaaggtggag ctggtaagaa
    26461 agaacgcttg cttagcttca gagaacaatt tttaaacaag aacaagaaaa aataaaagtg
    26521 agagccagct ctcgcttttc tcatagtggg aggtaaggat ggaattacgc agaccaagat
    26581 tagcggataa gaaagctgtt ttagatatga tgacagagtt tgaaaaattt cagtcgcctc
    26641 acgacggcgg tttctgggat acagagaact ttgtgtatga agactggtta gaaagcaatc
    26701 aggaacagga aatgggaatt aatctgcctg aaggatgggt tcctgcaatt cagttagtgg
    26761 ctttttctga gaaaggtcaa gcagttggat ttcttaatct ccggttgcgc ctcagtaact
    26821 ttctactaga agaaggtggg cacattggct actccatccg tccatctgaa agaggcaagg
    26881 gttatgcaaa agagactctc cgtcagggct tgcaagttgc taaggaaaag aacatcaaga
    26941 aagctctggt gacctgtagc gtgaataatc ctgctagcag agcagtcatt ctagcaaatg
    27001 gtggaatatt tgaggatgct cgcaatggag tcgagcgtta ttggatagag gtagcgaatg
    27061 aataatccaa aaccacaaga atggaaaagc gaggaactta gtcaaggtcg tatcattgac
    27121 tacaaggcct ttaactttgt tgatggcgaa ggcgtgcgta actctctcta tgtagcaggc
    27181 tgtatgtttc actgcgaggg atgttataat gttgcgactt ggtcttttaa tgctggcatt
    27241 ccctatacag cagaattaga agagcagatc atggcagatc ttggtcagcc ctatgttcaa
    27301 ggtttgactt tgttgggagg ggagcctttt ctcaatactg gcattctctt gccacttgtt
    27361 aagcggattc ggaaagaatt gtcagacaag gacatctggt cctggaccgg ctacacttgg
    27421 gaagaaatga tgttggaaac tccagataaa ctggaactct tgtcactgat tgatattctt
    27481 gtcgatggaa gatatgatcg aactaagaga aatcttatgc tccagtttcg aggttcgtct
    27541 aaccaacgaa ttatcgatgt gcaaaaatcg ctcaaaagtg ggcaagtagt gatttgggac
    27601 aagctcaatg acggaaaaga aagctatgaa caggtgaaga gagaatgaag aaaaaaggct
    27661 tagtagacca actagtctca gagatcgaga cggggaaagt caggacactg ggaatatacg
    27721 gtcatggagc ttcaggtaaa tcaacctttg cacaggaatt gtcccaagct ttagattcta
    27781 ctacagtaaa tttgctagaa acagatccct atatcacctc cggacgctat ttggtagtac
    27841 ccaaggacgc gccgaatcaa aaggtgacag ccagtctgcc agtggcgtat gaactggaga
    27901 gtttgcagag agatatcttt gccttgcagg cgggtatgga tgtcttgaca attgaagaac
    27961 cttggaaggc tagtgaagtc ttgtctggag ccaaaccaat tttgattgtc gaagggatgt
    28021 ctgttggttt tctacccaag gaactctttg aaaaaaccat ctgtttctac acggatgagg
    28081 agaccgaatt aaagcgacgc cttgctagag atacgactgt gagaaatcgc gatgcatcct
    28141 ttatattggc tagccatcag atgagacggg agcagtatct gcgatactat aaagaaactg
    28201 agtctaaggc ggatatctta gtggaccaat cagaagataa atttgatgtc aagaggactt
    28261 aacttatgta tagaagaaaa tccctaattt tagaaagaaa aagtaggaaa acagtttcat
    28321 ctcaaaaaaa cgaaaaaacc taacaaatcc cttgcaatcg caggggattt gtgttattct
    28381 attatggtgc tgtaaattac agccttagtt ttgatgcaag aggttgcgac acgctcggtt
    28441 gcattgccac gcaacgccgc gtcggttttc ttgtggagct agcctattat cttaaataga
    28501 cgaaaaggag aaaaagatgg caaacaaaaa aatccgtatc cgtttgaaag cttacgaaca
    28561 ccgtacgctt gacacagcgg ctgcaaaaat cgtagaatca gctactcgta caggtgcaca
    28621 agttgcgggt ccaatcccac ttccaactga acgtagcctc tacacaatca ttcgtgcgac
    28681 tcacaaatac aaagactctc gcgaacaatt tgaaatgcgt acacacaaac gtttgatcga
    28741 tatcgttaac ccaactcaaa aaacagttga tgccttgatg aaattggatc ttccaagtgg
    28801 tgtaaacgta gaaatcaaac tttaatctaa aatataaaag agcagaggct ggtgtttcaa
    28861 tctaattgaa cacgggctaa actcggtgtg aaaaagataa acttcctcgc gtctgctaga
    28921 cactgcgtta gtttcctatt ttcactttga gtttgacgcc ctttgtatct tagacttgag
    28981 cataaaaaac gctcgttaaa aactttttga ataaaaaata tagaaaagga actattttct
    29041 catgacaaaa ggaatcttag ggaaaaaagt gggaatgact caaatcttca ctgaagctgg
    29101 cgaattgatc cctgtaacag ttattgaagc aactccaaac gttgttcttc aagttaaaac
    29161 tgttgaaaca gacggataca acgctatcca agttggtttc gatgacaaac gcgaagtatt
    29221 gagcaacaaa cctgctaaag gacatgtagc gaaagctaac acggctccta agcgcttcat
    29281 tcgtgaattc aaaaacgttg aaggcttgga agttggtgct gaaattacag ttgaaacatt
    29341 cgcagctgga gacgttgttg acgtaactgg tacttctaaa ggtaaaggtt tccaaggtgt
    29401 tatcaaacgc cacggacaat cacgtggacc aatggctcac ggttctcgtt accaccgtcg
    29461 tccaggttct atgggacctg ttgcacctaa ccgcgtattc aaaggtaaaa accttgcagg
    29521 gcgtatgggt ggcgaccgcg taacaattca aaaccttgaa gttgtacaag ttgttccaga
    29581 aaagaacgtt atccttatca aaggtaacgt accaggtgct aagaaatctc ttatcactat
    29641 caaatcagca gttaaagctg gtaaataata aagaaagggg aaatcagtca caatggcaaa
    29701 cgtaacatta tttgaccaaa ctggtaaaga agctggccaa gttgttctta gcgatgcagt
    29761 atttggtatc gaaccaaatg aatcagttgt gtttgatgta atcatcagcc aacgcgcaag
    29821 ccttcgtcaa ggaacacacg ctgttaaaaa ccgctctgca gtatcaggtg gtggacgcaa
    29881 accatggcgt caaaaaggaa ctggacgtgc tcgtcaaggt tctatccgct caccacaatg
    29941 gcgtggtggt ggtgttgtct tcggaccgac tccacgttca tacggctaca aacttccaca
    30001 aaaagttcgt cgcctagctc ttaaatcagt ttactctgaa aaagttgctg aaaacaaatt
    30061 cgtagctgta gacgctcttt catttacagc tccaaaaact gctgaatttg caaaagttct
    30121 tgcagcattg agcatcgatt ctaaagttct tgttatcctt gaagaaggaa atgaattcgc
    30181 agctctttca gctcgtaacc ttccaaacgt gaaagttgca actgctacaa ctgcaagtgt
    30241 tcttgacatc gcaaatagcg acaaacttct tgtcacacaa gcagctatct ctaaaatcga
    30301 ggaggttctt gcataatgaa tttgtatgat gttatcaaaa aacctgtcat cactgaaagc
    30361 tcaatggctc aacttgaagc aggaaaatat gtatttgaag ttgacactcg tgcacacaaa
    30421 cttttgatca agcaagctgt tgaagctgct ttcgaaggtg ttaaagttgc caatgttaac
    30481 acaatcaacg taaaaccaaa agctaaacgt gttggacgtt acactggttt tactaacaaa
    30541 actaaaaaag ctatcatcac acttacagct gattctaaag caatcgagtt gtttgctgct
    30601 gaagctgaat aatctaagga ggaaatatcg tgggaattcg tgtttataaa ccaacaacaa
    30661 acggtcgccg taatatgact tctttggatt tcgctgaaat cacaacaagc actcctgaaa
    30721 aatcattgct tgttgcattg aagagcaagg ctggtcgtaa caacaacggt cgtatcacag
    30781 ttcgtcacca aggtggtgga cacaaacgtt tctaccgttt ggttgacttc aaacgtaata
    30841 aagacaacgt tgaagcagtt gttaaaacaa tcgagtacga tccaaaccgt tctgcaaaca
    30901 tcgctcttgt acactacact gacggtgtga aagcatacat catcgctcca aaaggtcttg
    30961 aagtaggtca acgtatcgtt tcaggtccag aagcagatat caaagtcgga aacgctcttc
    31021 cacttgctaa catcccagtt ggtactttga ttcacaacat cgagttgaaa ccaggtcgtg
    31081 gtggtgaatt ggtacgtgct gctggtgcat ctgctcaagt attgggttct gaaggtaaat
    31141 acgttcttgt tcgtcttcaa tcaggtgaag ttcgtatgat tcttggaact tgccgtgcta
    31201 cagttggtgt tgtcggaaac gaacaacatg gacttgtaaa ccttggtaaa gcaggacgta
    31261 gccgttggaa aggtatccgc ccaacagttc gtggttctgt aatgaaccct aacgatcacc
    31321 cacacggtgg tggtgaaggt aaagcaccag ttggtcgtaa agcaccatct actccatggg
    31381 gcaaacctgc tcttggtctt aaaactcgta acaagaaagc gaaatctgac aaacttatcg
    31441 ttcgtcgtcg caacgagaaa taatattaaa ctagtcgctt aagcaactag taaatccgcc
    31501 agctcggtag cgctccatag gagtgcaagc cgctgtggta caacatttaa aggagaaaat
    31561 ataaaaatgg gacgcagtct taaaaaagga cctttcgtcg atgagcattt gatgaaaaaa
    31621 gttgaagctc aagctaacga cgaaaagaaa aaagttatta aaacttggtc acgtcgttca
    31681 acgatcttcc caagtttcat tggttacact attgcagttt atgacggacg taaacacgta
    31741 cctgtttaca tccaagaaga catggtaggc cacaaacttg gtgaatttgc accaactcgt
    31801 acttacaaag gtcacgctgc agacgacaag aaaacacgta gaaaataagg agaacataaa
    31861 tggcagaaat tacttcagct aaagcaatgg ctcgtacagt acgtgtttca cctcgtaaat
    31921 cacgtcttgt tcttgataac atccgtggta aaagcgtagc cgatgcaatc gcaatcttga
    31981 cattcactcc aaacaaagct gctgaaatca tcttgaaagt tttgaactca gctgtagcta
    32041 acgctgaaaa caactttggt ttggataaag ctaacttggt agtatctgaa gcattcgcaa
    32101 acgaaggacc aactatgaaa cgtttccgtc cacgtgcgaa aggttcagct tcaccaatca
    32161 acaaacgtac agctcacatc actgtagctg ttgcagaaaa ataaggaggt aaaatcgtgg
    32221 gtcaaaaagt acatccaatt ggtatgcgtg tcggcatcat ccgtgattgg gatgccaaat
    32281 ggtatgctga aaaagaatac gcggattacc ttcatgaaga tcttgcaatc cgtaaattcg
    32341 ttcaaaaaga acttgctgac gcagcagttt caactattga aatcgaacgc gcagtaaaca
    32401 aagttaacgt ttcacttcac actgctaaac caggtatggt tatcggtaaa ggtggtgcta
    32461 acgttgatgc actccgtgca aaacttaaca aattgactgg aaaacaagta cacatcaaca
    32521 tcatcgaaat caaacaacct gatttggatg ctcaccttgt aggtgaagga attgctcgtc
    32581 aattggagca acgtgttgct ttccgtcgtg cacaaaaaca agcaatccaa cgtgcaatgc
    32641 gtgctggagc taaaggaatc aaaactcaag tatcaggtcg tttgaacggt gcagatatcg
    32701 cccgtgctga aggatactct gaaggaactg ttccgcttca cacacttcgt gcagatatcg
    32761 attacgcttg ggaagaagca gatactacat acggtaaact tggtgttaaa gtatggatct
    32821 accgtggtga agttcttcca gctcgtaaaa acactaaagg aggtaaataa ccaatgttag
    32881 tacctaaacg tgttaaacac cgtcgtgagt tccgtggaaa aatgcgcggt gaagcaaaag
    32941 gtggaaaaga agtagcattc ggtgaatacg gtcttcaagc tacaactagc cactggatca
    33001 ctaaccgcca aatcgaagct gctcgtatcg ccatgactcg ttacatgaaa cgtggtggta
    33061 aagtttggat taaaatcttc ccacacaaat catacactgc taaagctatc ggtgtgcgta
    33121 tgggatctgg taaaggggca cctgaaggtt gggtagcacc agttaaacgt ggtaaagtga
    33181 tgttcgaaat cgctggtgta tctgaagaga ttgcacgtga agcgcttcga cttgctagcc
    33241 acaaattgcc agttaaatgt aaattcgtaa aacgtgaagc agaataagga gaaggcatga
    33301 aacttaatga agtaaaagaa tttgttaaag aacttcgtgg tctttctcaa gaagaactcg
    33361 cgaagcgcga aaacgaattg aaaaaagaat tgtttgaact tcgtttccaa gctgctactg
    33421 gtcaattgga acaaacagct cgcttgaaag aagttaaaaa acaaatcgct cgtatcaaaa
    33481 cagttcaatc tgaagcgaaa taatagacta gggaaggaga aatttcaatg gaacgcaata
    33541 atcgtaaagt tcttgttgga cgtgttgtat ctgacaaaat ggacaagaca atcacagttg
    33601 tagttgaaac aaaacgtaac cacccagtct atggtaaacg tattaactac tctaaaaaat
    33661 acaaagctca tgatgaaaac aatgttgcca aagaaggcga tatcgtacgt atcatggaaa
    33721 ctcgcccgct ttcagctaca aaacgtttcc gtcttgtaga agttgttgaa gaagcggtca
    33781 tcatctaatc aaacctgaaa ggagaaaact gaaatgattc aaacagaaac tcgtttgaaa
    33841 gtcgcagaca acagcggtgc tcgcgaaatc ttgactatca aagttcttgg tggttcagga
    33901 cgtaaatttg caaacatcgg tgatgttatc gtggcatctg taaaacaagc tactcctggt
    33961 ggtgcggtta aaaaaggtga cgttgttaaa gcagttatcg ttcgtactaa atcaggtgct
    34021 cgtcgtgctg atggttcata catcaaattt gacgaaaacg cagcagttat catccgtgaa
    34081 gacaaaactc ctcgcggaac acgtatcttt ggcccagttg cacgtgaatt gcgtgaaggt
    34141 ggcttcatga agatcgtgtc acttgctcca gaagtacttt aatttttagg aacaaactag
    34201 tcccctagct tcaagctagg gtgcccttat gggcgtaaga aaaatcaagg agaaacctaa
    34261 tgtttgtaaa aaaaggcgac aaagttcgcg taatcgctgg taaagacaag ggaacagaag
    34321 ctgttgtcct tactgccctt ccaaaagtaa acaaagttat cgttgaaggt gttaacattg
    34381 ttaagaaaca ccaacgtcca actaacgagc ttcctcaagg tggtatcatc gagaaagaag
    34441 cagctatcca cgtatcaaac gttcaagttt tggacaaaaa tggtgtagct ggtcgtgttg
    34501 gatacaaatt tgtagacggt aaaaaagttc gctacaacaa aaaatcaggc gaagtgcttg
    34561 attaatcacg aaggaaagga gaagtataat ggcaaatcgt ttaaaagaaa aatatcttaa
    34621 tgaagtagtt cctgctttga cagaacaatt caactactca tcagtgatgg ctgtgcctaa
    34681 agtagataag attgttttga acatgggtgt tggtgaagct gtatcaaacg ctaaaagcct
    34741 tgaaaaagct gctgaagaat tggcacttat ctcaggtcaa aaaccactta tcactaaagc
    34801 taaaaaatca atcgccggct tccgtcttcg tgaaggtgtt gcgatcggtg caaaagttac
    34861 ccttcgtggt gaacgtatgt acgaattctt ggataaattg gtatcagttt cacttccacg
    34921 tgtacgtgac ttccacggtg tcccaacaaa atcatttgat ggacgcggga actacacact
    34981 tggtgtgaaa gaacaattaa tcttcccaga aatcaacttc gatgacgttg acaaaactcg
    35041 tggtcttgac atcgttatcg taacaactgc taacactgac gaagagtcac gtgcattgct
    35101 tacaggcctt ggaatgcctt ttgcaaaata atataggagg taaatctaat ggctaaaaaa
    35161 tcaatggtag ctagagaggc taaacgccaa aaaattgttg accgttatgc tgaaaaacgt
    35221 gctgcattaa aggcggcagg ggactacgaa ggtttatcta aattacctcg caacgcctca
    35281 ccgactcgtt tacataatcg ttgtagagtt acggggcgcc cacattcagt ttaccgcaaa
    35341 tttggtctga gtcgtatcgc ttttcgcgaa cttgcgcata aaggtcaaat tcctggtgta
    35401 acaaaagcat cttggtaatt taagatatca agggtgtcaa aactccaagt aaaaatagga
    35461 aacttgacga agaaactaaa gtttctagga aagtttatct ttttcacaca gagtttagcc
    35521 cgggttcaat tgggcttgcc aatttgaacc cgagctacag ctttggcaaa aaagaccaat
    35581 ttgctttgga gcattgcttc tgcattaaat tgtctatttt tgctcgtgct gttacgctct
    35641 ttgtatcatg tattaactag caagtgcaac ttgcaaacta ctagtaagag gagaaaaaca
    35701 aaatggttat gactgaccca atcgcagact tcctaactcg tattcgtaat gctaaccaag
    35761 ctaaacacga agtacttgaa gtacctgcat caaacatcaa aaaagggatt gctgaaatcc
    35821 ttaaacgcga aggttttgta aaaaacgttg aaatcattga agatgacaaa caaggcgtca
    35881 tccgtgtatt tcttaaatac ggaccaaatg gtgagaaagt tatcactaac ttgaaacgtg
    35941 tttctaaacc aggacttcgt gtctacaaaa aacgtgaaga ccttccaaaa gttcttaacg
    36001 gacttggaat tgccatcctt tcaacttctg aaggtttgct tactgataaa gaagcacgcc
    36061 aaaagaatgt tggtggtgag gttatcgctt acgtttggta aaatcaagat acaaagctcg
    36121 taaagaacaa agcaaaatta ggaagttgga gaagtttgtt tacaaacaag ccaacttatc
    36181 tattttgcac agttcttaga gcgtgttcag ttcagctctt gaactaaata agtatctgaa
    36241 ccccgtgaaa actggccgtt ctggcctgac aatttaacag gagaaaataa acatgtcacg
    36301 tattggtaat aaagttatcg tgttgcctgc tggtgttgaa ctcgctaaca atgacaacgt
    36361 tgtaactgta aaaggaccta aaggagaact tactcgtgag ttctcaaaag atattgaaat
    36421 ccgtgtggaa ggtactgaag taactcttca ccgtccaaac gattcaaaag aaatgaaaac
    36481 tatccacgga actactcgtg cccttttgaa caacatggtt gttggtgtat cagaaggatt
    36541 caagaaagaa cttgaaatgc gtggggttgg ttaccgtgca cagcttcaag gatctaaact
    36601 tgttttggct gttggtaaat ctcatccaga cgaagttgaa gctccagaag gaattacttt
    36661 tgaacttcca aacccaacaa caatcgttgt tagcggaatt tcaaaagaag tagttggtca
    36721 aacagctgct tacgtacgta gccttcgttc accagaacca tataaaggta aaggtatccg
    36781 ttacgttggt gaattcgttc gccgtaaaga aggtaaaaca ggtaaataat gttgagtggt
    36841 tgatcatcaa ccaccaacct attttccaac tttgtgcata gcacacgatt taaaactaaa
    36901 gaggtgaaaa ctgtgatttc aaaaccagat aaaaacaaac tccgccaaaa acgtcaccgt
    36961 cgcgttcgcg gaaaactctc tggaactgct gatcgcccac gtttgaacgt attccgttct
    37021 aatacaggca tctacgctca agtgattgat gacgtagcgg gtgtaacgct cgcaagtgct
    37081 tcaactcttg ataaagaagt ttcaaaagga actaaaactg aacaagccgt tgctgtcggt
    37141 aaactcgttg cagaacgtgc aaacgctaaa ggtatttcag aagtggtgtt cgaccgcggt
    37201 ggatatctat atcacggacg tgtgaaagct ttggctgatg cagctcgtga aaacggattg
    37261 aaattctaat aggaggacac tagaaaatgg catttaaaga caatgcagtt gaattagaag
    37321 aacgcgtagt tgctgtcaac cgtgttacaa aagttgttaa aggtggacgt cgtcttcgtt
    37381 tcgcagctct tgttgttgtt ggtgaccaca atggtcgcgt aggatttggt actggtaaag
    37441 ctcaagaagt tccagaagca atccgtaaag cagtagatga tgctaagaaa aacttgatcg
    37501 aagttcctat ggttggaaca acaatcccac acgaagttct ttcagaattc ggtggagcta
    37561 aagtattgtt gaaacctgct gtagaaggtt ctggagttgc cgctggtggt gcagttcgtg
    37621 ccgttgtgga attggcaggt gtggcagata ttacatctaa atcacttggt tctaacactc
    37681 caatcaacat tgttcgtgca actgttgaag gtttgaaaca attgaaacgc gctgaagaaa
    37741 ttgctgccct tcgtggtatt tcagtttctg atttggcata agaaagggga taaaatggct
    37801 caaattaaaa ttactttgac taagtctcca atcggacgca ttccatcaca acgtaaaact
    37861 gttgtagcac ttggacttgg caaattgaac agctctgtta ttaaagaaga taacgctgct
    37921 atccgtggta tgatcacagc agtatctcac ttagtaacag ttgaagaagt aaactaatga
    37981 agttttaggg gatgtgcact gtaccatccc ctaaaactag atatagtcat ctatgatgac
    38041 atcgtatagg cgagttgatg ggggagacaa ccttttctcc cttatcggcg ctagcatttt
    38101 acaaaagagg agaaaataaa aatgaaactt catgaattga aacctgcaga aggttctcgt
    38161 aaagtacgta accgcgttgg tcgtggtact tcatcaggta acggtaaaac atctggtcgt
    38221 ggtcaaaaag gtcaaaaagc tcgtagcggt ggcggagttc gccttggttt tgaaggtgga
    38281 caaactccat tgttccgtcg tcttccaaaa cgtggattca ctaacatcaa cgctaaagaa
    38341 tacgcaattg tgaaccttga ccaattgaac gtctttgaag atggtgctga agtaactcca
    38401 gttgttctta tcgaagcagg aattgttaaa gctgaaaagt caggtattaa aattcttggt
    38461 aacggtgagt tgactaagaa attgactgtg aaagcagcta aattctctaa atcagctgaa
    38521 gaagctatca ctgctaaagg tggttcagta gaagtcatct aagagaggtg acctatgttt
    38581 tttaaattat taagagaagc tcttaaagtc aagcaggttc gatcaaaaat tttatttaca
    38641 atttttatcg ttttggtctt tcgtatcgga actagcatta cagttcctgg tgtgaatgcc
    38701 aatagcttga atgctttaag tggattatcc ttcttaaaca tgttgagctt ggtgtcgggg
    38761 aatgccctaa aaaacttttc gatttttgcc ctaggagtta gtccctatat caccgcttct
    38821 attgttgtcc aactcttgca aatggatatt ttacccaagt ttgtagagtg gggtaaacaa
    38881 ggggaagtag gtcgaagaaa attgaatcaa gctactcgtt atattgctct agttctcgct
    38941 tttgtgcaat ctatcgggat tacagctggt tttaatacct tggctggagc tcaattgatt
    39001 aaaactgctt taactccaca agtttttctg acgattggta tcatcttaac agctggtagt
    39061 atgattgtca cttggttggg tgagcaaatt acagataagg gatacggaaa cggtgtttcc
    39121 atgattatct ttgccgggat tgtttcctca attccagaga tgattcaggg catctatgtg
    39181 gactactttg tgaacgtccc aagtagccgt atcacttcat ctatcatttt cgtaatcatt
    39241 ttgattatta ctgtattgtt gattatttac tttacaactt atgttcaaca agcagaatac
    39301 aaaattccaa tccaatatac taaggttgca caaggtgctc catctagctc ttaccttccg
    39361 ttaaaggtaa accctgctgg agttatccct gttatctttg ccagttcgat tactgcagcg
    39421 cctgcggcta ttcttcagtt tttgagtgcc acaggtcatg attgggcttg ggtaagggta
    39481 gcacaagaga tgttggcaac tacttctcca actggtattg ccatgtatgc tttgttgatt
    39541 attctcttta cattcttcta tacgtttgta cagattaatc ctgaaaaagc agcagagagc
    39601 ctacaaaaga gtggtgccta tatccatgga gttcgtcctg gtaaaggtac agaagaatat
    39661 atgtctaaac ttcttcgtcg tcttgcaact gttggttccc tcttccttgg tgtgatttcc
    39721 attttaccga ttgcagctaa agatgtattt ggtctttctg atgttgttgc ctttggtgga
    39781 acaagtctct tgatcattat ctctacaggt atcgaaggaa tcaagcaatt ggaaggttac
    39841 ctattgaaac gtaagtatgt tggtttcatg gacagaacag aataaaagta tttactgaat
    39901 cagtaaatac tgagggagtg gaggtttaaa ctctgacatt tgtaagagtt ggatctcccc
    39961 tcttctattt tgtttttaaa taggggtgaa aagacttttt gcttctattt aaaaataaaa
    40021 taaggagatc aaatcatgaa tcttttgatt atgggcttac ctggtgcagg taagggaact
    40081 caagcagcaa aaatcgtaga acaattccat gttgcacata tctcaacagg tgatatgttc
    40141 cgcgctgcaa tggcaaatca aactgaaatg ggtgttcttg ctaagtcata tattgacaag
    40201 ggtgaattgg ttcctgacga agttacaaat ggaatcgtaa aagaacgcct ttcacaagat
    40261 gatattaaag aaacaggatt cttattggat ggttacccac gtacaattga acaagctcat
    40321 gccttggaca aaacattggc tgaacttggc attgaactag aaggtattat caatattgaa
    40381 gtgaaccctg acagcctctt ggaacgtttg agtggccgta tcatccaccg cgtaactgga
    40441 gaaactttcc acaaggtctt taacccacca gttgactata aagaagaaga ttactaccaa
    40501 cgtgaagatg ataagcctga gacagtaaaa cgtcgtttgg atgttaatat tgctcaagga
    40561 gaaccaatca ttgctcacta ccgtgccaaa ggtttggttc atgacatcga aggtaatcaa
    40621 gatatcaatg atgtcttctc agatattgaa aaagtattga caaatttgaa ataaagcgtt
    40681 tttcgcactt gcaaaaatcc gctacaaatg ttatactgag atagtctgac ttataattgt
    40741 tgtctctgtg tctagaggca tcgaatcgaa atttatggag gtgcttttgc gtggcaaaag
    40801 acgatgtgat tgaagttgaa ggcaaagtag ttgatacaat gccgaatgca atgtttacgg
    40861 ttgaacttga aaatggacat cagattttag caacagtttc tggtaaaatt cgtaaaaact
    40921 atattcgtat tttagcggga gatcgtgtta ctgtcgaaat gagtccatat gacttgacac
    40981 gtggacgtat cacttaccgc tttaaataat cgaaaaactt ggagggataa gaaatgaaag
    41041 taagaccatc ggtcaaacca atttgcgaat actgtaaagt tattcgtcgt aatggtcgtg
    41101 ttatggtaat ttgcccagca aatccaaaac acaaacaacg tcaaggataa gatagaaagg
    41161 agaaaacatg gctcgtattg ctggagttga tattccaaat gacaaacgcg tagtaatctc
    41221 attgacttat gtttatggta tcggacttgc aacatctaag aaaattttgg ctgctgctgg
    41281 aatctcagaa gatgttcgtg tacgtgatct tacatcagat caagaagatg ctatccgtcg
    41341 tgaagtggat gcaatcaaag ttgaaggtga ccttcgtcgt gaagtaaact tgaacatcaa
    41401 acgtttgatg gaaatcggtt cataccgtgg tatccgtcac cgtcgtggac ttcctgtccg
    41461 tggacaaaac actaaaaaca acgctcgcac tcgtaaaggt aaagctgttg cgattgctgg
    41521 taagaaaaaa taatatagga ggtaaaagtc ttggctaaac caacacgtaa acgtcgtgtg
    41581 aaaaagaata tcgaatctgg tattgctcat attcacgcta catttaataa cactattgtt
    41641 atgattactg atgtgcatgg taatgcaatt gcttggtcat cagctggtgc tcttggtttc
    41701 aaaggttctc gtaaatctac accattcgct gctcaaatgg cttctgaagc tgctgctaaa
    41761 tctgcacaag aacacggtct taaatcagtt gaagttactg taaaaggtcc aggttctggt
    41821 cgtgagtcag ctattcgtgc gcttgctgcc gctggtcttg aagtaacagc aattcgtgat
    41881 gtgactccag tgccacacaa tggtgctcgt cctccaaaac gtcgccgtgt ataatcatcg
    41941 cattacactg cttttcgttt aagagggagt aactaaatga tcgagtttga aaaaccaaat
    42001 ataacaaaaa ttgatgaaaa taaagattat ggcaagtttg taatcgaacc acttgaacgt
    42061 ggctacggta caactcttgg taactctctt cgtcgtgtac ttctagcttc tctaccagga
    42121 gcagctgtga catctatcaa cattgatggt gtgttacatg agtttgacac agttccaggt
    42181 gttcgtgaag acgtgatgca aatcattctg aacattaaag gaattgcagt gaaatcgtac
    42241 gttgaagacg aaaaaatcat cgaactggat gttgaaggtc ctgctgaagt aacagctggt
    42301 gacattttga cagatagcga tattgaaatt gtaaatccag atcattatct ctttacaatc
    42361 ggtgaaggtt cttctctaaa agcgactatg actgttaaca gtggtcgtgg atatgtacct
    42421 gctgatgaaa ataaaaagga taatgcacca gttggaacac ttgctgtaga ttctatttat
    42481 acaccagtta caaaagtcaa ctatcaagtg gaacctgctc gtgtaggtag caatgatggt
    42541 ttcgacaaat taacccttga aatcttgaca aatggaacaa ttattccaga agatgcttta
    42601 gggctttcag cacgtatttt gacagaacat cttgatttgt ttacaaatct tactgagatt
    42661 gctaagtcaa ctgaagtgat gaaagaagct gatactgaat ctgacgaccg tattttagat
    42721 cgtacgattg aggaactgga cttgtctgtg cgttcataca actgtttaaa acgtgccggt
    42781 atcaatactg tgcatgattt gacagaaaaa tctgaagcag agatgatgaa agtacgaaat
    42841 cttggacgca agagtttgga agaagtgaaa ctcaaactca ttgatttggg tcttggatta
    42901 aaagataaat aaaggaggaa tacatggctt accgtaaact aggacgcact agctcacaac
    42961 gtaaagcaat gcttcgcgat ttgacaactg accttttgat caacgaatca atcgtgacaa
    43021 ctgaagctcg tgctaaagaa atccgtaaaa ctgttgaaaa aatgattact ctaggtaaac
    43081 gtggtgattt gcatgcacgt cgtcaagcag ctgctttcgt acgtaatgaa atcgcatctg
    43141 aaaactatga tgaagcaact gataagtaca cttctactac agcacttcaa aaattgttct
    43201 cagaaatcgc acctcgttat gctgaacgta acggtggata cactcgtatc cttaaaactg
    43261 aaccacgtcg tggtgatgca gcgccaatgg cgatcatcga attagtataa aatcatcaat
    43321 tttgttgagt gttatgatga tggagtcttg tgctcttagt ctagctctgg tctaccgcta
    43381 ggatttcggt cctagcggga acactcatca taagttggga tagtagacgc ttgtttacga
    43441 aattgttttt ttcttaagaa caacttcgta agcaggcgtt tttgagtatt ttcgttagaa
    43501 ttatgctata ctatttgaaa agaatcctgt ttaatgttaa ggtttcttat tttaagaaga
    43561 attggagttt acttatgaaa gccattataa ctgttgttgg taaagataaa tctggaattg
    43621 ttgcaggtgt ttctggtaaa attgcagaat taggattgaa tattgacgat atctctcaaa
    43681 ctgtcttgga tgaatatttt acgatgatgg ctgttgtatc tagtgatgaa aagcaagatt
    43741 ttacctatct tcgtaatgaa tttgaagctt ttgggcaaac tttgaatgta aaaatcaata
    43801 ttcagagtgc agcgattttc gaagctatgt ataatatcta ggaggtcatc atggatatta
    43861 gacaagttac tgaaaccatc gccatgattg aggagcaaaa cttcgatatt agaaccatta
    43921 ccatggggat ttctcttttg gactgtatcg atccagatat caatcgtgct gcggagaaaa
    43981 tctatcaaaa aattacgaca aaggcggcta atttagtagc tgttggtgat gaaattgcgg
    44041 ctgagttggg aattcctatc gttaataagc gtgtatcggt gacacctatt tctctgattg
    44101 gggcagcgac agatgcgacg gactacgtgg ttctggcaaa agcgcttgat aaggctgcga
    44161 aagagattgg tgtggacttt attggtggtt tttctgcctt agtacaaaaa ggttatcaaa
    44221 agggagatga gattctcatc aattccattc ctcgcgcttt ggctgagacg gataaggtct
    44281 gctcgtcagt caatatcggc tcaaccaagt ctggtattaa tatgacggct gtggcagata
    44341 tgggacgaat tatcaaggaa acagcaaatc tttcagatat gggagtggcc aagttggttg
    44401 tattcgctaa tgctgttgag gacaatccat ttatggcggg tgcctttcat ggtgttgggg
    44461 aagcagatgt tatcatcaat gtcggagttt ctggtcctgg tgtggtgaaa cgtgctttgg
    44521 aaaaagttcg tggacagagc tttgatgtag tagccgaaac agttaagaaa actgccttta
    44581 aaatcactcg tatcggtcaa ttggttggtc aaatggccag tgagagactg ggtgtggagt
    44641 ttggtattgt ggacttgagt ttggcaccaa cccctgcggt tggagactct gtggcacgtg
    44701 tccttgagga aatggggcta gaaacagttg gcacgcatgg aacgacagct gccttggccc
    44761 tcttgaacga ccaagttaaa aagggtggag tgatggcctg taaccaggtc ggtggtctat
    44821 ctggtgcctt tatccctgtt tctgaggatg aaggaatgat tgctgcagtg caaaatggct
    44881 ctcttaattt agaaaaacta gaagctatga cggctatctg ttctgttgga ttggatatga
    44941 ttgccatccc agaagatacg cctgctgaaa ccattgcggc tatgattgcg gatgaagcag
    45001 caatcggtgt tatcaacatg aaaacaacag ctgttcgtat cattcccaaa ggaagagaag
    45061 gcgatatgat tgagtttggt ggtctattag gaactgcacc cgttatgaag gttaatgggg
    45121 cttcgtctgt cgacttcatc tctcgcggtg gacaaatccc agcaccaatt catagtttta
    45181 aaaattaaga aaataggaga aattttaagt tctatttaag attagatgtg tatactataa
    45241 tcattaaata aagacctcct aatattattt gaaacagata acactgaatt agtttgaatt
    45301 tgattttcat ctaatatctt tatttaatga actcctaaac tttttcataa taatctcctt
    45361 caaaagtcgc ctgtatgggt ggcttttatt ttatcattca tgatataata gaagcaaacg
    45421 gaggacggaa aatggtaaaa gtacgattgt atttggtacg tcatggcaag accatgttta
    45481 acacgattgg tcgcgcgcaa ggttggagcg atactccctt aactgctgaa ggtgaacgag
    45541 ggattcaaga gttaggaatc ggtttgcgag aatctgatct acagtttgag cgcgcttatt
    45601 cgagtgattc tggtcgtacc attcagacca tgggaattat acttgaagaa cttggcttgc
    45661 aggggaaaat cccttatcgc atggacaagc gtatcagaga atggtgtttc ggtagttttg
    45721 atggagccta tgatggcgat cttttcatgg gcattattcc tcgtatcttt aatgtggacc
    45781 acgttcacca attgtcttat gctgaactgg ctgagggctt ggtagaggtc gatacagctg
    45841 gttgggctga aggttgggaa aaactcagtg gccgaatcaa ggaaggcttt gaaataattg
    45901 caaaagaaat ggaagatcaa ggtggaggta acgcccttgt tgtcagccat ggaatgacta
    45961 ttggaaccat tgtttatctg attaatggca tgcatccgca tggtctagat aatggtagcg
    46021 tgacgattct tgaatatgag gacggtcagt ttagggttga agttgtcggt gaccgtagtt
    46081 accgagagct aggacgtgag aagatggaag aaggttctat ttaatcagtc tagacttgct
    46141 tgccatgagc tagggatttg ataagaatat caagataaga aaaaacagcc gagggtactc
    46201 ctttcggctg tttttgatgt ggaaaactaa agtgtaatgc tattgctttt agagattttc
    46261 ataaacaaga gcaaggaacc tactgttaga acagtcagga tagttgacaa ggctgcggct
    46321 acaccgtaat ttcctctgag aatctctgta taaatagcca cagtcattgt tcttgttttg
    46381 acattgtaga ggaggataga agtagagagt tttgaaatca ttgtgaccca agataagatg
    46441 gctccagaaa tgataccaga tagcatcatt ggagttgtaa tcttagcaaa gatattgaga
    46501 cgactacttc ctaagctttc agcagcttct tcaatacttg gtgctatttg ttgtaagcta
    46561 gcaacagatg agcgaatagt ataaggtaat cttctgacag atagagacat aatcaagatg
    46621 aaagcagtcc ctgtaatcat aagaaatcca cttccaaata gaccagtatt gaaggaagaa
    46681 atgaaggcaa tccctagaac ggttcctggt acaatataag gtaccatact gaggctgtca
    46741 attaagtttg taaacaaatt ccgttttcta acggctaggt aggagataaa tgtcgcaaat
    46801 agaacaacta gaactaaggc aatcaaaggg atacgaatgg tattgaaaat agcagatccc
    46861 atacgattga aagctacctt gtaactgttt ggagaataac ctttaacaaa taccatacct
    46921 gatgttttta ggaaagaggt ataaattaag tagatttgag gtaaaacaga gataaagata
    46981 attccgtaga ctgttgcata aatggcagcc atttttcctt ttgtagtttt tttaggctca
    47041 attggatgga gcagattcat gctgaaactg tagcggtttg caatgtgttt ttggataagg
    47101 aaaattgcca aggcaatgat aatcgccata attgcaaaag cagaatttcc tccaacctcg
    47161 ctaataaatt gggtataaat caggacaggg aaagtccgat atccttcgcc aatcaacata
    47221 ggcgttccaa agtctgagaa tgctctcata aatacaagca aggagctgct agtaaggttg
    47281 aaactaggag aggtaaaaca accgttacga taggtttaaa tccgaaggcc cccatgcttt
    47341 cagctgcttc aagtagagaa ttgtcaatac tgttcattgt tccagcaaca tatagaaata
    47401 ccagtgggaa tagttgcagt gtaaagacaa gtatacttcc tttgaatcca taaatatcga
    47461 tagctggaag atgaagggca tttgtcaaaa atttagtgat gacctcattt cgtctcagca
    47521 agagaatcca ggagtaggct cctacgaaag gagctgacat ggaagcaatg ataatcaata
    47581 tttgtagaaa tttcttcccc ttgaagtcat acatagagaa gagataagct aatagggttc
    47641 ctacaactaa ggaagtgaca gtagcggtaa tggaaaccct gaaactgttg actagtgtct
    47701 cagagtagta ggttttacta aagaaagtga caaaattagc tagtgagaat tgtccttcat
    47761 gtataagtgc ttgcttgagc acggtaacga taggataaac gagaaagaca agataggtaa
    47821 gaaagatgaa gaaagaggaa gctgtccaaa tatttagttt tttacattcc atggttgact
    47881 ccttttatca ggttttggga accatctgca gaaaagatgt ttaatttttg cgtattgatt
    47941 cgtagacgaa tacgattgcc tttttgtagt tcttcttcaa aagttgattc ttcactaact
    48001 tgaatttttg aggcaaaacc tgtctcaatg aaatactccg tatttagtcc aagatagacg
    48061 ctatcgctaa tagttccttc aatatctcca gattcatctt tgataaactc ttcgggacga
    48121 atgcttacat gaatagcttg ctcctcaacc tgatcaagag ctggcattcg aagggcatag
    48181 ccatctgaaa agacgatata agcgccgtcg ctccgttttt caagattggc agggataata
    48241 tttgtgcgtc cgataaaggt tgccacaaac tcattagctg gtttatgata gagttctttt
    48301 ggtcggccga tttgttggat caccccatct ttcataacag caatttggtc tgaaatagcc
    48361 atggcttctt cttggtcgtg ggttacataa acagttgtaa ttcccacttc gtgttggatt
    48421 tctcggatgg cttgacgcat atccaagcga agtttggcct ccagattact aagtggctcg
    48481 tccatgagga gaacacttgg attaaccgct aaggcgcatg ccaaggtgac acgttgttgt
    48541 tgtccaccac tgagtttatc gggttttcga tccgcatatt gagcaatttg catgagttca
    48601 agatacttgt tggtctgttg aatcaattct tcttttgcat aagaccaaaa gcaacgttgt
    48661 ctcggacagt caaatgtggg aaaatagcgt agttttggaa aaccatcccg atattgcgtt
    48721 tgctgggttc catattattg atttttgtat catcgaagta aaattctcaa tctttgatac
    48781 tgttgaaacc tgcaatcata cgaagaaggg tcgttttccc acatcctgaa gctccaagaa
    48841 gggtaaagag acttcctttt ggaattgtaa tgttcaaatt ctcaataaca gggacatcgt
    48901 ggtagatttt tttggcgtta ataattttga tctcactcat agtgaacctc ttttactgtt
    48961 tagattggat atctgtaaag acttcgttgt atttcttaac gatatctgat ttattcttga
    49021 tgacataatc ataatcttca gtgagtgttt tgattttgtc aattggtttc atgttttcgc
    49081 ttgttttagc atttttacga acaggacggt tagtagtggt tgtaccaagt gtatcttgta
    49141 cttcttgaga gataataaaa tcgataaatt tcttggcatt ttccatattt ttagattttt
    49201 taacgatagc agcactagca ggtaggaaga cggttccttc ttttggatag actaccttaa
    49261 tgttagctcc gtcatttaag agtttaactg ctggatcttc ataagagaga ccaacagtca
    49321 tttctccatc agcgactgct ttatagacac cagatgaact taaaccgatt ttatagta
//
LOCUS       DAVBNO010000010        46527 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVBNO010000010 DAVBNO010000000
VERSION     DAVBNO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46527
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.010"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(<1..463)
                     /locus_tag="VQ846_000604"
     CDS             complement(<1..463)
                     /locus_tag="VQ846_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075237.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCG"
     gene            complement(453..650)
                     /locus_tag="VQ846_000605"
     CDS             complement(453..650)
                     /locus_tag="VQ846_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075238.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(654..1238)
                     /locus_tag="VQ846_000606"
     CDS             complement(654..1238)
                     /locus_tag="VQ846_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075239.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKKFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGDKANMDVVLVFSKLSDGEKKQLLQARVPFVDFKG
                     NLFFPTLGLVLNANDTEVPKELIPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(1636..2061)
                     /locus_tag="VQ846_000607"
     CDS             complement(1636..2061)
                     /locus_tag="VQ846_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075240.1"
                     /translation="MTSRVFIDADCISVFLWVGIEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEGASISLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(2190..2549)
                     /locus_tag="VQ846_000608"
     CDS             complement(2190..2549)
                     /locus_tag="VQ846_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075241.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMALG"
     gene            complement(2595..2801)
                     /locus_tag="VQ846_000609"
     CDS             complement(2595..2801)
                     /locus_tag="VQ846_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075242.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLER"
     gene            complement(2788..2949)
                     /locus_tag="VQ846_000610"
     CDS             complement(2788..2949)
                     /locus_tag="VQ846_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3075243.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(3308..4264)
                     /gene="lytA"
                     /locus_tag="VQ846_000611"
     CDS             complement(3308..4264)
                     /gene="lytA"
                     /locus_tag="VQ846_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET3075244.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKYDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(4640..6010)
                     /locus_tag="VQ846_000612"
     CDS             complement(4640..6010)
                     /locus_tag="VQ846_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET3075245.1"
                     /translation="MNKKRTVDLIHGSILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPRFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(6317..7483)
                     /gene="recA"
                     /locus_tag="VQ846_000613"
     CDS             complement(6317..7483)
                     /gene="recA"
                     /locus_tag="VQ846_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET3075246.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            complement(7538..8794)
                     /locus_tag="VQ846_000614"
     CDS             complement(7538..8794)
                     /locus_tag="VQ846_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET3075247.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(8879..9895)
                     /locus_tag="VQ846_000615"
     CDS             complement(8879..9895)
                     /locus_tag="VQ846_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET3075248.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(9903..10421)
                     /locus_tag="VQ846_000616"
     CDS             complement(9903..10421)
                     /locus_tag="VQ846_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3075249.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKITGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(10411..10854)
                     /gene="tsaE"
                     /locus_tag="VQ846_000617"
     CDS             complement(10411..10854)
                     /gene="tsaE"
                     /locus_tag="VQ846_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET3075250.1"
                     /translation="MYIKNEEELQALGERLGHLLAKNDVLILIGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLSEELQYGV"
     gene            complement(10940..11560)
                     /locus_tag="VQ846_000618"
     CDS             complement(10940..11560)
                     /locus_tag="VQ846_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075251.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(11943..12806)
                     /locus_tag="VQ846_000619"
     CDS             complement(11943..12806)
                     /locus_tag="VQ846_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3075252.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            13310..15325
                     /locus_tag="VQ846_000620"
     CDS             13310..15325
                     /locus_tag="VQ846_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET3075253.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDSNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLYVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAGLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            15327..15959
                     /locus_tag="VQ846_000621"
     CDS             15327..15959
                     /locus_tag="VQ846_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075254.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLTYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            16100..16378
                     /locus_tag="VQ846_000622"
     CDS             16100..16378
                     /locus_tag="VQ846_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HET3075255.1"
                     /translation="MKKFDNYIVEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(16887..17300)
                     /gene="ndk"
                     /locus_tag="VQ846_000623"
     CDS             complement(16887..17300)
                     /gene="ndk"
                     /locus_tag="VQ846_000623"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET3075256.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(17411..21088)
                     /gene="rpoC"
                     /locus_tag="VQ846_000624"
     CDS             complement(17411..21088)
                     /gene="rpoC"
                     /locus_tag="VQ846_000624"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET3075257.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPYAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(21121..24732)
                     /gene="rpoB"
                     /locus_tag="VQ846_000625"
     CDS             complement(21121..24732)
                     /gene="rpoB"
                     /locus_tag="VQ846_000625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET3075258.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            25148..25218
                     /locus_tag="VQ846_000626"
     tRNA            25148..25218
                     /locus_tag="VQ846_000626"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25180..25182,aa:Cys,seq:gca)
     gene            complement(25416..26747)
                     /locus_tag="VQ846_000627"
     CDS             complement(25416..26747)
                     /locus_tag="VQ846_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET3075259.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(27038..27862)
                     /gene="endA"
                     /locus_tag="VQ846_000628"
     CDS             complement(27038..27862)
                     /gene="endA"
                     /locus_tag="VQ846_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET3075260.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(27901..28089)
                     /locus_tag="VQ846_000629"
     CDS             complement(27901..28089)
                     /locus_tag="VQ846_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET3075261.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(28082..29365)
                     /gene="murA"
                     /locus_tag="VQ846_000630"
     CDS             complement(28082..29365)
                     /gene="murA"
                     /locus_tag="VQ846_000630"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET3075262.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(29389..29619)
                     /locus_tag="VQ846_000631"
     CDS             complement(29389..29619)
                     /locus_tag="VQ846_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET3075263.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(29677..30714)
                     /locus_tag="VQ846_000632"
     CDS             complement(29677..30714)
                     /locus_tag="VQ846_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET3075264.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHLDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(30698..31186)
                     /gene="coaD"
                     /locus_tag="VQ846_000633"
     CDS             complement(30698..31186)
                     /gene="coaD"
                     /locus_tag="VQ846_000633"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET3075265.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(31176..31715)
                     /gene="rsmD"
                     /locus_tag="VQ846_000634"
     CDS             complement(31176..31715)
                     /gene="rsmD"
                     /locus_tag="VQ846_000634"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET3075266.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(31780..32772)
                     /locus_tag="VQ846_000635"
     CDS             complement(31780..32772)
                     /locus_tag="VQ846_000635"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET3075267.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(32883..33566)
                     /locus_tag="VQ846_000636"
     CDS             complement(32883..33566)
                     /locus_tag="VQ846_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET3075268.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(33622..34362)
                     /locus_tag="VQ846_000637"
     CDS             complement(33622..34362)
                     /locus_tag="VQ846_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET3075269.1"
                     /translation="MTIITSKANSVVKNAKKLRQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSPALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            34396..34674
                     /locus_tag="VQ846_000638"
     CDS             34396..34674
                     /locus_tag="VQ846_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET3075270.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            34752..35678
                     /gene="yidC"
                     /locus_tag="VQ846_000639"
     CDS             34752..35678
                     /gene="yidC"
                     /locus_tag="VQ846_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET3075271.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(35753..36547)
                     /gene="pflA"
                     /locus_tag="VQ846_000640"
     CDS             complement(35753..36547)
                     /gene="pflA"
                     /locus_tag="VQ846_000640"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET3075272.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(36673..37923)
                     /locus_tag="VQ846_000641"
     CDS             complement(36673..37923)
                     /locus_tag="VQ846_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET3075273.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(37990..38817)
                     /gene="purR"
                     /locus_tag="VQ846_000642"
     CDS             complement(37990..38817)
                     /gene="purR"
                     /locus_tag="VQ846_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET3075274.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(39084..40025)
                     /locus_tag="VQ846_000643"
     CDS             complement(39084..40025)
                     /locus_tag="VQ846_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET3075275.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(40015..41271)
                     /locus_tag="VQ846_000644"
     CDS             complement(40015..41271)
                     /locus_tag="VQ846_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET3075276.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(41273..41935)
                     /locus_tag="VQ846_000645"
     CDS             complement(41273..41935)
                     /locus_tag="VQ846_000645"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET3075277.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDGFVGVDRGSLWILE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFKQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(41898..42554)
                     /locus_tag="VQ846_000646"
     CDS             complement(41898..42554)
                     /locus_tag="VQ846_000646"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET3075278.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDGHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVSNPEHHLEDFVRAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRDEKGLNFEIEVDGGIDDQTIAQSKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(42565..43443)
                     /gene="rsgA"
                     /locus_tag="VQ846_000647"
     CDS             complement(42565..43443)
                     /gene="rsgA"
                     /locus_tag="VQ846_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET3075279.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDGGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     TAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIAPFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(44036..44809)
                     /locus_tag="VQ846_000648"
     CDS             complement(44036..44809)
                     /locus_tag="VQ846_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HET3075280.1"
                     /translation="MRKKLVNSLMCAVVLSGFPIATYANEKNIGPNITVSNGQTRLSI
                     PEKNGVEVIQSEENRVVYEVKEAAKIETSDGDMVAPIVVDSRITSINNQVVQGKTVYT
                     ADLEKATFVEEGYNKLAKPTNILDHIFGIQKVHAYTQNITTHQSQFDSTYSVELYTVV
                     HWVSYDGGVQKNITKISGGVDFHERDIEIESSSVEVRQGGWYSSQQKWFHPGARSSWE
                     YSTGFSKVGPGENRVVTNYTVNLIRYGDRWSAYLQNNPW"
     gene            complement(45499..46371)
                     /gene="rsmA"
                     /locus_tag="VQ846_000649"
     CDS             complement(45499..46371)
                     /gene="rsmA"
                     /locus_tag="VQ846_000649"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET3075281.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAEVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPVPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLE
                     EFASLADALKGQGL"
BASE COUNT        13620 a        10375 c         8594 g        13938 t
ORIGIN      
        1 gaccacaatt actcaatacc gaatacccct gaatggtttc tttttcatgc accaatatat
       61 tataataatc ttcatccaat aataaggctg ataaactagc atcttggtca aaatgtaagg
      121 gaatttctcg accgtccttc tttaatggat attctggtaa gatactaaat agttcaatca
      181 tagaaggaaa ctcagaatta gttgttgtaa atcgaaaaag ctgcgctttc tcatctttct
      241 gacttccttg atactctccc aattctaaaa aatgattcaa ggtagtataa aattccttat
      301 tttttacttc atcaatgatg accatatcat aatcttttgt tgtgcgactt ttaaatcctt
      361 gcgaatccaa tacgatagag gtagcagttc ccccaatcag aacataatag ttctgaaaat
      421 ccgcaaacat ttcttgaaaa ataactttcg tattagctgg catcatcttc tcccagatac
      481 tgtaatatca tattttctag tgcttcactc tcttcctcta tacgtgggtc atcatcatct
      541 tttaaggtca aataaagaga aatcggatct acaaattgtt tatcatgatt atttttaaaa
      601 tcattccaaa actcagatac aaaaggacga tatttccata tctctagcat ctttcatttt
      661 aaaacatgct gagaaagtgg caaggataac tgattgaatt ttctctgcca tatgacatag
      721 ctagtatttt catccgtttc agctaaaaaa gttgaatgcg acaaagcata agcaccacca
      781 tatagaaggt tagaaacaga ttttatctgc tttatatcgc catctggcaa taaaaaggca
      841 atccacgtta attgttcgct aggtattaat tccttaggga cttcagtatc attcgcattc
      901 agtactagtc ccaatgtagg gaagaagagg tttcccttaa agtctacaaa cggaactcta
      961 gcttgaagta attgcttttt ttcaccgtct gataacttcg aaaacaccaa aacaacatcc
     1021 atattggctt tatcacccat agtgcgagcc tgagtaacaa atgaactcaa actccccctt
     1081 ctcttttctt taatcaataa aaatgattcc ttatgaaaag tcatttgagt atactcaaac
     1141 ttttttacaa aactgattgg taaagtcaaa gtcagttcct caatactgac atcaacataa
     1201 aaggattgaa aaattgacat tatctttttg acattcatgt tttttcctta ttctatcatt
     1261 tttattatat tttatcattc ggtataataa aatataagtt ttttgataaa ataaccaaaa
     1321 actactcgct atcccctctc acattaccct acatatcgtt tgacatgcga ctgatagttc
     1381 aggaaaactt caaggagaac ttttctctca tccactatgc aggacttact atttcacttc
     1441 tactcctata ggctcaattt gacatttttc tttttcgaat tgctcatatg cctcctgaat
     1501 agtgagttcg atttatccaa atttattttt gtctattttg atgaatactt ccacgaagaa
     1561 tgaatttgca ccaattttcc ttctcttttt aagcatatta ttccagatat gattgccctc
     1621 ttgttcagta ataaattacg ctttaaacgc ttcaatcagt atatctcctg ttgtcatatg
     1681 ttctaaagaa aattcttcta catatggttt aacatctctt aggttattac ttcctaatat
     1741 cccattatgc tttttcgcta aggaaataga tgccccttct cctttaccaa taatcttgtt
     1801 actatcatga tttcttgtta aatctctata taatgcgtat tcttcagttc caatgtctat
     1861 gctcacaatc tcagctgaac ccttagctac caactgatct atcctagatt ttaaatgggg
     1921 aattgtaggt atattgattt catcatacac ctcttgtgga ataacaattt tacccaaata
     1981 gagcttttct aaaagatgtt caatgccaac ccataaaaat actgaaatac aatctgcatc
     2041 aataaataca cgactagtca aacgacaact cccccctctt catctagccc atatacaata
     2101 tcatatctga aagcatctag taacagttcc tcatacttcc cttgcgaaat tctgttattt
     2161 tctaaaagtt gttcagttga attaatatat tatcctaatg ccatttcttt tttactttct
     2221 gacaaaggac gatataaact tgtatcatag cctaatcttg aagctgtctc tataacacta
     2281 atatccatat ttttaatttc ttctgcatca aggtatccat cattcctcaa tctatataac
     2341 atagctttat gactgatacc ataaaactga cccaatttta taatatcttc tacttcaaga
     2401 tgagttctat tggcattttc tctgatttcc tcaaccatcc tatacagtga agatgggaaa
     2461 attaaaaaat aagaagcaaa ctgatccgct tttctttcag tttcatctcc ttcaccaatc
     2521 aagataagac tgactgaact cttcttcacc tcatcataat aaagatgata cagtccatgt
     2581 gctaaagaaa atctttacct ttctaatggc atgtctgaat tgactgcaat gagactgaac
     2641 tgagttcctt tataacagac tccgctaata ttctttccga gtccataaaa taccagcgtc
     2701 aaattttcta tcttttgtac caatttaaaa atatctatcg gcgattcacc atcagctccc
     2761 aacttttttc taagatttga agctttatta cttaaatcca ttcgtgatat tcctttcacc
     2821 tttttccatt ttggcaatca tgctttgatt caaagacaaa tactcagcaa tcttatcttg
     2881 agtaagattt gaagatattc taagttcttt tattcttttt ccaacatcac atacatttat
     2941 cacatacatt tatcatattc atacacctct cataaaaaga atagcacact cttgtcataa
     3001 ttttttaaat aaaaaaatta tgacaaaaca aggaagcaat ttattgatgc tgcttaaaaa
     3061 tctaaaattg atgagttcct atcaaaaatc gtatcttcaa cctcaaaaca gtacttaaag
     3121 ctatccgact cggtttacat tgtcaaattt agattttatt tgagcataac tttctagttt
     3181 gctttctgat ttttgtttaa tatagtagca aacattgaag gaaatatctc cacaaaaaaa
     3241 cgcatactat taagcttttt caagacctaa taatatgcgc tgttctgatt tgaaagacat
     3301 tccattatta ttttactgta atcaagccat ctggctctac tgtgaattct ggcttgtctg
     3361 ccagtgttcc gtctggtttg aggtagtacc agcctgttcc gtccgctgac tggataaagg
     3421 catttgatac catggcgcct tctttagcgt ctaagtagta ccaagtgtcc ttgtacttga
     3481 cccagcctgt cttcatggca ccttcttcgt tgaaatagta ccacttatca gcgattttct
     3541 tccagcctgt agccatttcg cctgagttgt cgaaccagta ccagttgccg tctgtgtgct
     3601 tcctccagcg gtctgcaagc atatagcctg aactgtcaaa gtagtaccaa gtgccattga
     3661 ttttctcaaa cttgtctttt ggataagagc cgtctgaatg tacgtaccag tagccagtgt
     3721 cattcttctg ccagcctgtt tcaatcgtca agccgttctc aatatcatac ttaaactgct
     3781 cacggctaat gccccatttt gccaagtaag ggtatggatc cacatggtct gagtggttgt
     3841 ttggttggtt attcgtgcaa tactcgtgcg ttttaattcc agctaaactc cctgtatcaa
     3901 gcgttttcgg caaacctgct tcatctgcta gattgcgtaa gagttcgata taaaggcggt
     3961 aatccgtcat gaactcttct ttagttgaat ggctttcaat cagttcaacc gctgcatagg
     4021 tctcagcatt ccaaccgccc ccaacgtccc aggcaccatt atcaacaggt cctacctgca
     4081 tgatgcaacc gttcccaaca atgtgcgaga aaaaacctaa ttctgggtct ttccgccagt
     4141 gataatccgc ttcattctgt acggttgaat gcggattccc agttgagtgt gcgtgtactt
     4201 gcctatatgg ttgcacgccg acttgaggca aatctgttct taatttactc acattaattt
     4261 ccatattcta ctccttatca attaaaacaa ctcatttttt acaatccaga accagaaact
     4321 cctttatttc taccttacaa agaagacaat cttagtcacg attaggcttg tagatagaac
     4381 ctcaaaacgc actattttga cactgtaaat aggactgaca aggtctgcat tctatctaca
     4441 ataacacccc agactaaaaa gcttttcaaa agtatatttt tacagtctct atatgtcctt
     4501 ttcataaata ctatacttta ttatatcata taaaagaagt caaaagtctg ttaaactatt
     4561 ttcaacacca aactaaagaa gagaacacaa gttttttcga tgttcactag aggaaatgga
     4621 ttttattcag taaatccaac taggattgca ctttggttgc caaaattgcc tttccttctt
     4681 ttatcaaggg atgacggaat agtgagaagt acagttgaac tgtcatggca acccagataa
     4741 gaggttcaca aaggataaca cccttatatc ctgcccaagg aataatcaaa accacaaaaa
     4801 cgatttttcc gattagttca ataaagctag aaactagagg aaggatcttc tgccccaagc
     4861 cctgcaagca attgcgataa atcaacaaga ggctcaaaat gggataaaag gctgaactga
     4921 tttgcagata gagacttcca ttttctatca agtaaccatc tgtcgaacta gccaagaagg
     4981 aaaccaaggc tggactggca aaaaagagga aaatacaaac aaaaactgcc caggatatac
     5041 ttaaacgact gccgattcga agaccttgaa caatgcggtc aggtcgctta gctcctagat
     5101 tctgagaagc aaaggtcgtc attgatgcag aaatagcggt cataggaaga agggcaaagg
     5161 tcataatgcg tcgagctgcc gtctgggcac taataatcac tgcaccaaat gtattaacag
     5221 aaaactgtaa aatcacactg ccgatagata caattgaact catcaagccc atagccaaac
     5281 cttgctccaa gagatccgcg tacaagcttt tgtcccattt gaaatgttta aaccgtggca
     5341 agagttctgg cacactttta cgaatataat aaaagcagag aaccgctgat aaaccttgcg
     5401 aaataatggt agcaagtcct gcggattgaa ctcccagatg caattgcgta ataaaataga
     5461 gatccagaac cacattaacc aaggcagaga aaatcagaaa tcccagggct gctagactgt
     5521 caccaataga ccgcaacaag cctgcaaaaa gattataagc aaagctgaca cctacacagg
     5581 tcacaatcat agaaatatat tgataagatt gaggaagaat ttctgcagga gtatctaagt
     5641 attgcaagag aggatacaag ccaagaaagc ccagcaacat aactagaatg ctcaaaagag
     5701 cacctaaaat ccaggtggct gctactgctt ccttgatttt agtgaaattc cgagccccat
     5761 aataacgagc aatgacaatc cccatgccat tgccaacacc aagtgtaaaa cctacaatca
     5821 ggtcaaaaat cgctgtcgtc gctcctactg cagccaagga ttcttgacca agaaatcgtc
     5881 caacaatcaa gacatcagca gtgttataga gctgttgaaa aatatttgat agcaagattg
     5941 gaaaggtgaa gcttaagagc gagggaagaa tcgaaccatg tatcaggtcc actgttcgtt
     6001 ttttattcat aaagctatta tatcagcttt ccagaggagc gacaagaaac agcaaactta
     6061 tatatagcgg aatgaaataa ggacagtcaa atcgattttt aacaatgttt tagaaataga
     6121 tgtgtactat tctagtttcg atctattata gttggttgag tttggaatag tacgctgtag
     6181 ctgctaaaac atttctagaa attaatttga ctttcctaat aaagttgttc atatcttatt
     6241 tcaagctact atatataatc taaaaacttg aatcaaaaat cgaaaaagta gcggattttt
     6301 ccactgcttt aacagcttat tcttcaattt cgatttcaag ttcatcgcct aagtcaagcg
     6361 taacttcttc attcactgct tcttctttct ttggctcatc ttttttgttt tcagtatctt
     6421 gttctgaaac ttcttctcca tcaatcaagc caaatttaga acggacttgc ttatcaattt
     6481 catcaaagat ttctgggtgc tctgccaagt atttcttagc attctcagaa ccttgcccaa
     6541 ttttttcatc tttgtaagaa taccaagccc ctgctttttt gataatatcc aaatcgcttg
     6601 caatcttcaa aagctcacca gtcttagaaa ttccttctcc gtacataatt tcaactacgg
     6661 cttccttaaa cggtggagct accttatttt ttacaacctt aatcttagtt tctttaccga
     6721 cattggtttc tttttggtca ccagttccct taatttgtgt attaccacga acatccaagc
     6781 ggactgaagc atagaatttc aaagcacgtc cgcccggtgt tgtttctgga tttccaaaca
     6841 tcactccaac tttttcacgc aattggttga taaaaatggc aattgttttg gttttattga
     6901 tagaggcgcc aagtttacgc atggcctggc tcatcatacg agcctgcaaa ccaacatggc
     6961 tatctccgat atctccatca atttccgcac gaggaacaag ggcagcaact gagtcgacta
     7021 cgacaagatc aactgcacct gagtcaatca attttcccgc aatttcaaga ccttgctctc
     7081 ctgagtctgg ttgagacaag agcaattcgt caatattgac accaagggcc gcagcataag
     7141 ctggatcaag ggcatgttcc gcatcgataa aggcagcaat cccaccttct ttttgcgctt
     7201 gtgcaactgc atgaagggca accgttgtct taccagatga ctctgggcca tagatttcga
     7261 tgatacgtcc cttaggataa ccacctgagc caagggcaat gtcaagagct aaagaacctg
     7321 agctcatcac ttgcaccttt tgctccgcac gttcacccaa acgcatgatt gatcctttac
     7381 caaagtcttt ctcaatcaat ttaagagcgt cattcaaggc cttttcacgt tctgccccaa
     7441 atttttttga aatttcttct aatttttttg gttttttcgc cattctattc tcctacattc
     7501 taatgatcct cagacctatc tactattata tcaaaagtta gtcacttaat aaagccttgc
     7561 gaactaggtt aaaggcatgc ataaccgcaa tatgacgtac atctgctcgg cttctgcctc
     7621 caatattaac cttgataacc tcagttcctt gctcttgcgc caagcctatg aagactgtcc
     7681 caactgggtg tccttctagg ctatctggtc ctgccactcc ggtcaaacta attccaaaat
     7741 cagactgggt cttgcttcgt gcctgctcag ccatcttctg agctgtaaat tcagacacca
     7801 caccatgttc ttccaaattc ttggcaggaa tatccaacat ccttgatttt tcctccaagc
     7861 tataggtcac aaaaccaccc ttaaatatac ttgaaactcc agaaaaattc gccacggtag
     7921 cttggaaaag acctgccgtc aaactctctg cagccgcgat ggttttccct tgccttttca
     7981 gttcttctac cacaatgctg gctaaactag tttcttcccc ataaccatag caaaagtctc
     8041 gtaaagaaat tccttcgaaa gtctggcagt ccaagatttg attttccaag atatccagcg
     8101 cttgattcgc ctcttcttga ctgctagcct ttgttgacag acgtagagtg acttctcctg
     8161 tcttggcata aggggccaag gtaggatcga tctgattatc aattaaatca gccaaaatcg
     8221 taaccagctg gctctcgcca atcccaaaga aacgaagaac tcgggaatac agcttgctcc
     8281 ctgtcatcaa cttgggtaga agttggttta agaccatggg tttcaattca cttggcggac
     8341 ctggaaggac gacataggtc actccgtcta cttctaattt tcctccaaca gccagtcctg
     8401 tttcgtttgg cagtggaatc gctccttcta caatttgagc ttgtctttcg ttattcggtg
     8461 ttcgggcata gtctggtcgc agggcaaaaa agatatccaa cttctcctga gcctgaggat
     8521 caaagactaa tgctttccct aaaaatttag ctagggtttg tttggttagg tcgtcctcag
     8581 ttggccccaa accgcctgtc aaaatcacca gactgctacg ttgactggca atctcaagca
     8641 aagacaagag acgaacttca ttgtctccta cagccgtctg aaaatataca tctaccccaa
     8701 tctcagctag tttttccgac aaaaactggg cattggtgtt gacaatctgt cctgtcaaaa
     8761 tctctgttcc aacagcaatg atttctgctt tcatgtttcc tcctacctat ctattcgtat
     8821 ttttttgaaa aaatcgcagg aattttccta cgattgattt ttttatttgt atcaaaagtt
     8881 aattatcttc atcaccaaca ggtgctctgc caaataaatc ttcaaataaa accgcattgg
     8941 tttcaagctg agtaacttct tcttgtccca aagaacgtcg gagtatattt tgcatttcca
     9001 acatatgtgc tctcgaaaca atctggtaag aaacaccttg aagtatctct ccttcaccct
     9061 gcaactgctg agtttcaatg gttttaaatg aatctttata gcctagcaag ttagggatac
     9121 tttttgcaga caaatcaata ttggtctgca tattgtcact caaagctttt agaatctctt
     9181 gataatgacc aatgctattt aaactgagag ctttttccat gactttttga ataacttcac
     9241 gttgacgttt ttgacgacca taatccccct caggatcttg gtaacgcatt cgtgcataga
     9301 ctagggcttc ttctccccca atatgttgct ccccaacacc gatagaaatg gtattaaatt
     9361 cttcttggtc actgatagaa attgggaaac ctaggatatt attgactgta atacctccta
     9421 ctgcatccac taatttttgc aaccctctca tattgaccat cacatagcga tcaatatgga
     9481 tattcatcat tttttgaatg gtttctatag caagctctgc tccaccatct gcatatgctg
     9541 agttcagttt cgcttcatga gcctgaccat tccctgattc aatgcgcgtc agaatatccc
     9601 gctctaaact catcattgtt gtttttttcg ttttaggatt cactgtcatc aagatcatgc
     9661 tatcacttct accgacccaa gtttcagttc gttcaacatt tccggtgtcc actcccatta
     9721 acagaatggt tagaggttca gtcgcttcaa taaccttggt ttcttcgccg atttttttat
     9781 aggttttagc taaggtttct gtcccttgtt gataaatagt ataagcaaaa acacctactc
     9841 ctactacagt tacagaaagt aaagctagca ccattccaat aattttttta accatatttc
     9901 tactaaccta tcagtttacc catcaagtaa acatcgataa atttcccttc ttctatatat
     9961 gccccacgct cttggctacc ttcaatgaca aagccatgct tttgataaag atggactgct
    10021 gcttgattac gagtttggac agtcagttgg agacgacgca gaatgccact tgcttgtgcc
    10081 cactctatcg cttcttctag caacaaactt cccaagccat tattccaata tctttttcca
    10141 atcacaatga agagatctcc aatatgacgg actctcttac gctgatcagc tgtaatattt
    10201 acaataccag taattttgcc atttaagaat gcaagtaagg ttatctgatt gtccgaacta
    10261 gcttgcttgt tgaggaatat ttccatctcc tcactagtca agagaatacc atctccgtct
    10321 aggctggtaa agtctgtctc caaactcaca cgatttaaaa aggccactaa ttcagctgca
    10381 tctttgggct ctgcttccct aatgagcaat tcatactcca tattgaagct cctctgacaa
    10441 tttctcagca cgcaaaccct ttgcctgaaa atttaaacgg cgtccatctg cttcttttag
    10501 aatttccaat tctaaataag catctggcaa ggcatctcct aagagatttc cccactcaat
    10561 aacagtcacg ccgccaccaa agataaactc atccaagtcg atagaatcag catctccttc
    10621 aatacgataa acatctaggt gataaagtgg aagtcgacct tcatactctc tcacgatagt
    10681 ataggtagga cttttaatca tttgagaaat ctgtaatcct tttgcaagtc ctttagtaaa
    10741 ggtcgtttta cctgcaccca gttctccaat taagattaaa acatcattct ttgctaatag
    10801 atggcccaaa cgctccccta aggcttgcaa ctcttcttca ttttttatgt acatactctt
    10861 attataccaa aaacttttct tttgtgtcta ttttcctact aaacttatca tcataacatc
    10921 cataaaaaac aggctttctc taaaagaaaa tgagcgtaac aatgaccaat acaagatctc
    10981 ggaaaatatg accataaaag gaaacttcct tcttaaccga atttgggaca agataggctg
    11041 caaaaaacaa gcccagtcca atataaatca gaagtgagac aatggtcatt ggatttctta
    11101 agaaaagaag tgttgctaaa atagtcacca acactgtctt ttttctgtcc agcatagcaa
    11161 gaaaatcgcg cacgtatttt ttcaagggta aaaaaatcag caaatctagc ccaaatagga
    11221 aaaagaagga tggcaataaa aagtcaacta attcttgctg cagcgtattt ttgatgaaca
    11281 agttatctga caaaacaaga acagctccta acaaattaat taagagtaac atactgtaaa
    11341 aaagcttcac cgacttctta ctggctagga cactatggac ttcttgctta cgagtataaa
    11401 gataatttac tccagcacag attcctgaaa cgaaaaccat gcttccgatg aaaaaagctg
    11461 tactttgttt aaaggacaag atgcattcct tccataggaa acagctactc aaactgattt
    11521 gaattaaagc taacaaaaat aagattctca ttgatttcat cttctctctc ccttcctacc
    11581 aatcattata ctaggagaaa agagagaact gtttctaatc ttctcaaatg tctctttaag
    11641 acgctaaaca aacactagag actaatactc aatgaaaatc aaagagcaaa ctaggtagct
    11701 agccacaggt tgctcaaaac actgttttga gattgcagat agagctgacg tggtttgaag
    11761 agatttacga agaatataaa tttgaaatca tgaaaatccg tcaaacgggt ggttgttttg
    11821 tctcgcacct cacggagcga gacggactca gagtcacata attataaggc tgatagtatt
    11881 aatctaacta tcagccttac aggttattta acgtttcaga aaaactataa tgtcaagatt
    11941 aactaaacag tatctagttc cttcaaataa ttttctatct tcatcaacat taaaggattg
    12001 ttataaatct tacataactc tcttgcttct atataataat ttttgacttg ttctctgtct
    12061 agaaatttgg ctccagcatt tcctacaaga ataagtagag gagccaattg gtagcttgtc
    12121 tgtctttgtt tacagagttc aatcgtttca agagcttctt ggatggcttc attatatttt
    12181 tcctttgata ctaggtagtg agcgtagttg taacgaactc tgatgtagcc aaataaaaac
    12241 tcttgatggt ccaaattttt tgtctgatac aactctatta aatgagagta gtttgcctca
    12301 tattcttgtt cacgacccac taaggaatag aaattagata gagtattcaa cgcctttaaa
    12361 taaatcagag tatttgaaga gacttttaat aatatatttt ccaatgaaga aattgcctca
    12421 cacttactgt catattgata gaagtcaata atagctttaa tccattcaag gtaagttcgg
    12481 tcttctagtg ttagaaaagt acttcgttca atctctattc tataaatata ttccaaatcg
    12541 tcataatttc tatcgtctaa tagtcgagca gacaattgct tgaaattaga gaggttagat
    12601 ttaatttcaa tttgttcatt aaaaaaataa tctaatggta cttcaagtcg ttgagagagt
    12661 ttgaacaaaa ggtctgcgga gggaatgaaa tgccctctct caattttact aatctgactt
    12721 tgttcacaaa ttccttctgc aagagtttgt tgggagagtc tcaactcttt tcttttcaat
    12781 ctgaatttct ctgcgagtgt attcattaaa gataataaac cttcctattt gcttaattgc
    12841 attataaaat ttttagttca aaatagcaag ttaaaagaat aaataattct ttatggtaat
    12901 aaactaaaaa aagtcaaaaa ttacataaaa atgcttgacc gaataatata tatatattaa
    12961 aataaaaaca aggaaaaaga aaagggtttc acgaatgaag aaaaatcgtg gaattcaaaa
    13021 attagctata ttagtattac taggtgtttt tatgcttagt aacacaattc cttaccaaca
    13081 gtttattcag aagaatagac aattggagat tcgagtccaa tcacaaaaga agtccaatgg
    13141 tcttgatgtt gggaaggctg attgatttcg tattacttta aaattcaact atttagagaa
    13201 aggtattctg atgagagctt gtaacaatac cagatagata ttagtaagct tgttagttta
    13261 tagagggcag gggtatttct gccctctgtt ttagaataag gaatgcacta tgaaaaaatt
    13321 atttatactc atatccaacc tgcttgctag tttgttcttt gtttgggtat ttaccatttg
    13381 gactgataca tatgtctcct attattaccc caacgttgtt gtacatgatt cttcccctga
    13441 aacaactttt cagcatgttg ctacacgctt ggaaaaacta gcagaagaaa cagatagctt
    13501 tattgcgatt caacaccaag attctaactc agaaggtact acagtttttt cttatacgac
    13561 ctttggggac ggaaagttgc ctgatgggct tcaggaaaaa aaactagaag atgctcaaag
    13621 tagtagtgtt gaaacaaact attttgtatt cgatggacac ctagatattc acttgctaag
    13681 ggaggagcta agtcaacttg gcttgactaa catgaacctg acaattccat ctaaactatc
    13741 tacgttaatg gctatcttta gtaacggatt tcagttaatc agtctattga ttttcatctt
    13801 aacctttgta gcactgactt taattagtca aattagccaa ttacggtcgt cgggcattcg
    13861 cctcatttca ggagagaaac ggtggtccat tttccttaga ccagttggtg aggatttgaa
    13921 agggatagct gttggtttca gtttagctgg cgtgctcgcc attcttatgc aaaaaattct
    13981 gtcgcttcca acgcaatccc taatgaccat aggagcaggc ttgctcagtt ataatctcat
    14041 cttgttgtcg atctccctgt ttttcgctca actcttcgca gttgggataa aaaagataca
    14101 cctgatgcag attataaaag ggcaagtgcc tgtcagagga atcattagtt tgattttaat
    14161 cggtcaacta ttagcgatta ttattgtaac gctgggaata gggagtagct taaagtattc
    14221 ccaagcctgg caacagcatc ggattggaca agaggcttgg agtcaggaaa ggcaactgat
    14281 taccctatca atcagccgtg aaggaacgag tcctggtttt gatgaacaag ctcaaaggaa
    14341 actcagaact tggtatcaat tgatggatct ggctgtttca gaacaaaagg ctttcttatc
    14401 tagacaccag ttaattgacc gtactttgca aaatggcatg gcttcctcca aaaacttgat
    14461 aacctctaca gaatggcacg attacaaccc gaatggcaat gtccttatcg tcacaccgca
    14521 atacttggag cgtcaaaaca ttcctgtaga tacaactatt gagcaaaaga tgaatcacct
    14581 ttatgtaggg gagtttgtct tattgctgcc tgaacacctc cgttcagagg aagaacatta
    14641 taaatctgtt tttgaagacg acttaaccag tcgcatgtct agtcaagatg aacgacagca
    14701 aatgactgct acggtaggtt atttagaatc aggtcaggat cgttttgtgt ataatacgac
    14761 ccctatttct taccagcagt ttttgaaaga tccaatcatc attgttataa caccccaatc
    14821 aactggtcca cagtccattt tgttttggat agacgcagta cagaactacg ttctctttaa
    14881 tcaattgtct gatgcccagg agcttatcca gagacaaggc attgaaaatt gggtctcaga
    14941 aatgcaaaca ggttaccaca actacatcac attattggat aatattcaga gggaacgttg
    15001 ggtaatgcta gcaggagctg ggcttgggat tgcaacttca atcttgttgt ttaacactat
    15061 gaataggctc tactttgaag aatttagacg tgccattttt atcaaacgca ttgcaggtct
    15121 caggttctta gaaatccatc gcacttatct ctttgctcaa ctgggtgtgt ttttactggg
    15181 atttgttgcg agtgtatttc ttcaggtaga gataggagtt gctttcttag tcttgttact
    15241 ctttactggt ctatctcttt tacagttaca tgtccaaatg cagaaagaaa acaagatgtc
    15301 catgcttgtt ttgaagggag gttaatatga ttgaacttaa acaggtgagt aaatcttttg
    15361 gagaacgaga gttattttcg aatctttcaa tgacatttga ggctggaaaa gtctatgcct
    15421 taattggttc aagtggtagc ggaaaaacaa ccttgatgaa catgattggg aaattagaac
    15481 cttatgatgg gacgattttt taccgaggta aagacttggc caattataaa tcgagtgatt
    15541 ttttccgtca cgaattgggc tacctcttcc agaactttgg cttaattgaa aaccaaagta
    15601 ttgaagaaaa ccttaagcta ggtctcattg gtcaaaagtt gagtcggtcg gaacagcggt
    15661 tgaggcagaa gcaggcttta gaacaggtcg gcctgactta tcttgaccta gataagcgca
    15721 tctttgagtt atcgggcgga gaatcgcaac gggttgcctt ggcaaaagtt atcttaaaga
    15781 atccaccctt tattctggca gatgagccaa cagcttcaat agacccagca acttctcagt
    15841 tgattatgga gattttgcta tctcttcgag atgataatag gctaatcatt atcgcaacac
    15901 ataatccagc aatttgggag atggctgatg aagtgttcac gatggatcgt ctgaaataaa
    15961 aatccttgtt tttaattgca cgatgagtta ctgaaatatt atcatgaatc aataattgga
    16021 gttaatttag aattgtactt tattaatatt gaggtaactt tttcttgata aaggaagaaa
    16081 tagtggagag gaagttagaa tgaaaaaatt cgacaattat attgttgaga agccttgcga
    16141 ttctaattca gataaactgc aaaaaatctt aataattgaa aatttggtag atgatatttt
    16201 gcaatttcct ctcagaatca ataatagtgt aggagagatt ttcctcctac aaccgtttca
    16261 aaagaaaact atctttattc catgttattt tgaggaagat attgtgaaag tcaaagatga
    16321 tgataaagtt gagtggaatt tgttagaatt tcaaaaattt agagcatttt tggcttagta
    16381 atctgtgttg aaggctcaaa acctatggta aaaaagtcgc tttgaaaacg tattgcctcc
    16441 aaagatttag ttaaataatg atttaacaca aaaagaaatt attgaagttc tggaaagatg
    16501 ttgtttcagt attgagaaaa ggtgggaaaa acttgcgatt ttcacagaga aaggaagaaa
    16561 aagtatagaa atatagtcaa ttgaaacaag aacaggacaa aagaaccttt tgtgccatat
    16621 ttttctcctt tcgctttaca attggcttga acacctttat tgtatcgcgt ttggagtttt
    16681 tttggtataa ccttcgacgc gcacccgcat agcgggtgtt tttttgtctc gcacctaacg
    16741 gagcgagacg aactaatagt cacttaatca aaaaacgcac catatcaaaa actaaaaagt
    16801 ttgatatcat gcgtcatgtc ttaaactaat tgactatact ttctattcaa atgagctttt
    16861 aaccaattga ttgagccaat ccactcttaa aaccaaagag caatttctcg cttagctgac
    16921 tcttctgaat ctgaaccatg tacaacattt tggataacct cattttctcc agcagctttt
    16981 gcaaaatcac ctcgaatagt gcctggtaaa gcttcttctg gacgagttgc acccatcatg
    17041 gtccgccaag tttcgattac tttgggacca gaaatgacac ccacaagaac tggacctgaa
    17101 gtcatgaatt cacgaatcgg tgggtaaaaa ctctgaccaa ccaagtcctg atagtgctgg
    17161 tcaatcaact cttctgaaac ttgtgaacga aactccaatt tttcgattgt aaatccacgt
    17221 tgttcgatgc gctttaacac ttcacccact agccctcttt ttacaccatc tggtttgatg
    17281 ataaagaatg tttgttccat acccgtctcc tttgtcagct tctttctttt attttaccac
    17341 atttcgtgga aaaatggaga aagttttcag aatagagaat gagagaaccc gagggttctc
    17401 tcattctcta ttattctact gtttcttcca cagtttcaac ggcagtatcc acaactactt
    17461 ctgttgtttc ttcatttcct tcttcctcta ctggaggatt aaggtattct tcttcgttga
    17521 cagcatatgg ttcaaggtta cggtaacggg ccataccagt accagctggg atgatcttac
    17581 cgatgataac attttcttta agtccaagga gatggtcttt cttaccacgg atagctgcgt
    17641 cagtaaggac acgagttgtt tcctggaagg aagccgctga caagaaactg tttgtttcaa
    17701 gtgaggcttt ggtaattccc ataaggactg ggcgacctgt cgctggaact ccacctgcga
    17761 taaggacatc tttgttggca tctgtaaagt cattgatatc catgagggta cccatgagaa
    17821 gatctgtatc acctggatcc atgacacgga ctttacggat catttgacga accattacct
    17881 cgatgtgttt gtcaccgatt tctacccctt ggctacggta aactttttgt acttcaccga
    17941 gaaggtacgt ttcaactgac aagacatcac gaactgcaag gagacgtttt ggttggatag
    18001 aaccttctgt cagagcagca ccacgcgcta cttggccccc aacttcgaca cgcatacgag
    18061 ctgtaaatgg aacgacatat tcaccttcgc cagtttcacc cttaacaaag actttcttgg
    18121 tacgagttga tgcatcttct tcgatagcag taacttgtcc tttaacctct gtgataaccg
    18181 cttccccttt aggattgcgg gcttcaaaga tttcttggac acgaggaaga ccctgagtga
    18241 tatcggtatt tgaggcaacc ccacctgtgt ggaaggtacg cattgtaagc tgtgtaccag
    18301 gttccccgat agattgggca gcaatggtac caactgcttc accaacttca accgcatcac
    18361 cagtcgccaa gttgataccg taacagtgac ggcagacacc gtgacgagtg ttacatgtaa
    18421 atacagaacg gatagtcact tcttccacac cagcattgac aatttcacgc gccttgtctt
    18481 ctgtaatcaa ttcatttgga ccaataatca ctgcaccagt ttctggatgt ttaacagttt
    18541 tcttagtgta acgaccgttg agacgctctt cgagagactc gatcatctct tttccttctg
    18601 cgatagaacg gatcaagaga ccacggtcag ttccacagtc gtcctcacgg atgataacgt
    18661 cttgggcaac gtcgaccaaa cgacgagtca agtaacctga gtcggctgtc ttaagggccg
    18721 tatcggtcat acctttacga gcaccgtgag ttgagaagaa catttccaat accgacaaac
    18781 cttcgcggaa gtttgaaagg attggcaatt ccatgatacg tccattcgga gcagccatca
    18841 gaccacgcat accggcaagc tgtgagaagt ttgagatgtt accacgggct ccagagtcca
    18901 tcatcataac gattgggttc ttaggatctt ggttagcaat caagcgtttc tcaagttttt
    18961 cacgggcagc acgccattca gctgtaacag cattgtaacg ctcgtcgtct gtgatcatac
    19021 cacgacggaa ttgtttggtg atttgttcga cacgtttgtg tgattcttca atgatttcag
    19081 tcttgtcatc aacgactggg atatcggcaa tacccactgt caatcctgca agagttgagt
    19141 ggtggtaacc gaggttcttc atgcggtcaa gtagggcaga agtttctgtc gtacggaaac
    19201 gtttgaagat ttcagcgatg atatttccaa ggtttttctt cttgaatgga gggttgagct
    19261 caagattgct gatagcttcc ttgatatctc caccaagtgg caagaagtat ttagctggaa
    19321 caccttctgt caagttggca ttgtttggtt cttgcaagta tggtagcccc tctggcatga
    19381 tatcgttgaa gagaatttta ccaactgttg taagcaagac cttatgtctt tgctcttctg
    19441 tccaaggctt gttgaggctg tctgttgcga taccaacacg tgagtggagg tgaacataac
    19501 cattgcggta agccataacc gcttcgtcac ggtctttgaa gaccattcct tcaccttcgc
    19561 gaccagcttc ttccatggtc aagtagtagt tacccaaaac catgtcctga gatggagtaa
    19621 ctaccggttt cccatctttc gggttcaaga tgtgctcagc agctagcatg aggatacgag
    19681 cttctgcttg tgcttcttct gaaagtggta cgtggatggc catttggtcc ccgtcaaagt
    19741 cggcattgta ggcttcacag acaagtgggt gcaagcgaag agccttacca tcaattaaga
    19801 ctggctcgaa ggcttggata cccaaacggt gaagggtcgg tgcgcggttc aaaagcactg
    19861 ggtgttcttt aatcacttct tcaaggatat cccagatacg ctcatctccg cgttccacca
    19921 agcgtttagc tgctttgacg ttttgcacga tatcacgggc aacgatttca cgcatgacaa
    19981 atggtttaaa gagttcaatc gccatttcac gtggcacacc acattggtac atcttaagag
    20041 ttggaccaac ggcgataacg gaacgtcctg agaagtcaac acgtttaccg agcaagtttt
    20101 gacggaagcg tccttgttta cctttaagca tgtggctcaa tgatttcaat ggacggctac
    20161 ctggtcctgt gattggacga ccacgacgac cattgtcaat caaagcgtca actgcttctt
    20221 gaagcatacg tttctcattt tgaacgatga tacctggtgc atttaactca agcaaacgag
    20281 ccaaacggtt gttacggttg ataacacggc ggtaaaggtc attcaagtca gatgaggcaa
    20341 aacggccacc atccaactgc aacattggac gaagatctgg tgggataacc ggaaggatgt
    20401 taagaatcat ccattcaggt ttgtttccag acttgtaaaa ggcatccaaa acatccaaac
    20461 gacggatggc tttgacacgt ttttgtccag tagctgtttt caattcttct ttgagttcag
    20521 caatttcttt ttcaagatct acttgcttca aaaggtcttg gatggcttcc gcacccatct
    20581 tggcaacaaa tgaaccataa ccatattcgc gcaagcgctc tcggtattcg cgctctgtca
    20641 tgatagactt gtgctcaagt ggtgtatcct taggatcaat caccacataa gccgcaaagt
    20701 agataacttc ctcgagggca cgagggctca tatcaagggt caagcccata cggcttggaa
    20761 tccccttgaa gtaccagatg tgagatacag gagctttcaa ttcgatatgt cccatacgct
    20821 cacgacgaac tttcgtacgc gttacttcaa ccccacagcg gtcacaaaca attcctctgt
    20881 aacgaatgcg tttgtactta ccacaagcac attcccagtc ttttgtagga ccaaagatca
    20941 cttcatcaaa gagtccttca cgttctggtt tcaaggtacg ataattgatt gtttcaggtt
    21001 ttttgacttc tccataagac catgaacgga ctttacttgg agaagctagg gtgatttgca
    21061 tacttttaaa acgatttaca tcaaccacta tttcttccct ttctattcta agtgaactgc
    21121 ttattcttgt tcagcagctt cttctgttgc ttccgctttt gttgctttct cagcttcttc
    21181 agcttcaaag gctgctttag cctcttgggc tgctttttcg cgggcttttt caaggtcatc
    21241 tacgtggatg acatcttcgt ccattccttc atccaagtcg cgaagttcca cttcttggtc
    21301 atcttcgtct aggacacgca tgtcaagacc aagagattgc aattctttga caagaactcg
    21361 gaaggattct ggaacacctg gttttggaat tggtttgcct tttgtaatag cttcataggc
    21421 tttcaaacgt ccgttgatat cgtccgactt gtaagtcaag atttcttgaa ggacatttga
    21481 cgcaccgtag gcttcaagag cccaaacctc catctcaccg aaacgttgtc caccaaactg
    21541 agccttacct ccgagtggtt gttgggtaac agttgagtat ggtccgactg aacgcgcgtg
    21601 caatttatca tcaaccatgt ggtggagttt gatcatgtac atgactccga cagaaacacg
    21661 gttatcaaac ggttcaccag tacgtccatc gtaaaggatc gttttggcat cgctatccat
    21721 acctgcttct ttaacagttg accaaagatc ttcagaactt gctccatcaa agactggtgt
    21781 cgcgatgtga ataccaagag tacgagctgc cataccaagg tgaagctcca taacctgacc
    21841 gatattcata cgtgatggta ccccaagtgg gttcaacatg atgtcgactg gagttccgtc
    21901 tggaaggtaa ggcatgtctt ctacaggaac gatacgagag acaacccctt tgtttccgtg
    21961 acgtccggcc attttatctc cgaccttaat cttacgtttt tgagcgatgt aaacacgaac
    22021 caacatgtta acacctgatt gcaactcatc tccatttaca cgtgtaaaga tcttaacatc
    22081 acgaacgaca ccatcggcac cgtgtggtac acgaagagaa gtatcacgca cttcacgaga
    22141 cttgtctcca aagatagcgt gcaagagacg ttcttcagct gaaagatctt tctcaccctt
    22201 aggtgttact ttacctacaa gaatatcacc ttctttaacc tcagcaccaa tacggataat
    22261 ccccatttcg tcaaggtctt tgagggcatc ttcaccaacg tttggaattt cgcgagtgat
    22321 ttcttcaggc ccaagctttg tatcgcgcgt ttctgattcg tattcttcaa ggtgaacaga
    22381 tgtgtagaca tcgtccttca ccaagcgttc gctcatgata acggcatcct cgaagttgta
    22441 accttcccaa gtcatgtagg caacgattgg gttttgtcca agcgccattt ctccattttc
    22501 catagaaggt ccgtcagcga tgaaatcgcc tttttcaacg acatcaccaa cttttacgag
    22561 agtgcgttgg ttgtaagcag tacctgagtt tgaacgacgg aatttttgga tgtggtaaac
    22621 atccaatgaa ccatcttcac gacgaacttc taccttgtca gcatctgcgt aagtaacttt
    22681 accatcatac tgagcaatca cagccgcacc agaatcgtgg gctgcttggt attccatacc
    22741 agtaccaacg taaggtgcct gaggattaat caatggcaca gcctgacgtt gcatattggc
    22801 tcccatgagg gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac
    22861 ggcaactacc tgttttggtg aaacgtccat gtagtcaaca atattagctg gatactcttg
    22921 gttgacccct tggtgacgtc ccatgacaat cttctcagca aaggttccat cttcattcag
    22981 acgagagtta gcctgagcta cagtatattc atcttcttca tcagctgtca accaaacaat
    23041 ttcgttcgtg acaacacctg tttcacggtc aaccttacgg tatggtgttt gaacaaaacc
    23101 atatttgttc aagtgtccgt aagatgacaa gttattgatc aaaccgatgt taggtccttc
    23161 aggtgtctcg attggacaca tacgaccata gtgagtgtag tgcacgtcac gtacttcata
    23221 tccggcacgg tcacgagtca aaccaccagg tcctaaggct gacaaacggc gtttgtgaga
    23281 caactcagaa agcgggttgt gttggtccat gaactgtgac aactgtgatg aaccaaagaa
    23341 ttctttaact gcagctgtta caggacggat attgataatt tgttgtggtg tcaagacttc
    23401 attgtcctga acagacatac gttcacggac attacgttcc atacgagaaa gtcccaaacg
    23461 tacttggttg gcaagcaatt caccaaccgc acggatacga cgatttccaa ggtggtcgat
    23521 atcatctaca cggccaagtc cttcagccaa gttgaggaag tagctcatct cagcaaggat
    23581 atctgcagga gtcaccgtac gaaccttgtc atctgggtta gcattaccaa tgatcgttac
    23641 gacgcgatct ggatcagttg gagcaacaac cttgaatttt tgaagaacaa caggctcagt
    23701 cacaacggct gcatcgtttg ggatgtagac aatcttgttc aagtcgccat ccaaatggct
    23761 ttcaatgctt tcaatcacgc tacgagtcat aatcgtacca gcttctacca agatttctcc
    23821 agtttcaggg tctaccaatg gctctgcaat ggtttggttg agcaaacgtg ttttaacatt
    23881 gagtttttta ttgattttgt aacgaccaac tgctgccaag tcatagcgac gtgggtcaaa
    23941 gaagcgagcc acaagcaagc tacgtgagct ttcagccgtc ttaggctcac ctggacgaag
    24001 gcgttcgtaa atttctttca aggcttcgtc tgtacgagag tccattggat tcttgtggat
    24061 atctttttca acagtgttgc gaaccaattc gctgtcacca aaaatatcaa agatttcatc
    24121 atcacctgag aaaccaagag cacgaaccaa ggttgtaaat ggaatcttac gagtacggtc
    24181 gatacgagtg taggtgatat cttttgagtc gctttcaagt tccaaccaag ctccacggtt
    24241 agggataaca gttgaaccat agcccacctt accatttttg tctactttgt cgttaaagta
    24301 aacacctggt gagcggacca actgagaaac gataatacgt tcaccaccat tgatgatgaa
    24361 agtacccatt tctgtcatga ttgggaaatc accaaagaaa acttcttggg tcttgatttc
    24421 gcctgtttct ttattgatca agcggaaggt tacaaaaatt ggtgctgagt agctagcatc
    24481 gtgaatacga gcttcttcta gcgtgtattt tggttccttg atttcatatc caacaaattc
    24541 caactccatt gtgtctgtga agtttgaaat tggcaataca tcttcaaaca cttccttaag
    24601 accgtggtct aggaaagctt tgaatgagtc agtttgaatt tcaatcaaat ttggtaagtc
    24661 aagaacttct ttgattcttg aaaaactacg acgggtacga tgtttcccgt attgaacgtc
    24721 atgtcctgcc aagatgattc tcctttgtaa ataagttcca agccttgtca atcaggcttt
    24781 tctaatcgcc atatggttgt aaacccctta tcaccgtgtc ctcttgacga attttcagaa
    24841 tctttaagcc tctgttacaa atgctcaaaa tcttgaaaaa aagcacaaaa agagcagcta
    24901 aatctgactt tttcagaaga tttaactgct gtgagccttg tctggacaat atttcagaca
    24961 aaacctacga caaatgatta cccatattat accctattta gctagatttt tcaaggggat
    25021 tcagtaggtt tttggtaatt tttttcccat agaaaacttg gcatcacatt cgaatcacgc
    25081 tatggtacaa aaaactgaaa aaactattga ctgaaaatca ttttcaaggt ataataataa
    25141 acgttaaggc ggtatagcca agtggtaagg cacggctctg caaaagcttg atcgtcggtt
    25201 caaatccgtc taccgccttc tataacttga tttatcaggt ttcaaatgaa cagaaagccc
    25261 aatttgaagg gcttttttta ttttccctcg aataaatacg tataacttta aaaacttttg
    25321 gagcgagttt gtggcagagt tctttccatg gcataattcc cttttgaaat caattgaaaa
    25381 aagcttcgtc acgcctggtg acaaaagcct ctagtttatt ctgtttcttc ttctatttcg
    25441 acttctgtga tttggacttt taccttggta acacgtccat tcttcacttt atcattggtt
    25501 aagataaact gtttgttttg gctgaccaat tcatagctga gtttctcagt cgttggaatg
    25561 gttcccactc ctgtcaaata ataaccagcg atggtatcaa cgtcatcact ttccagttca
    25621 acatcaaagt aattattgaa atcattgagg gtcatagttc cttgaacaat gtaggtatcc
    25681 tcgccaatct gatgaacatc tattgctgct ttgtccgttt catcatcaat ttcaccaaca
    25741 atttcctcta agaggtcttc caaagtcacc aaaccagcca taccaccgta ttcatccagc
    25801 aagattgcca tttgtctttg ggtatttcgc agttctttta gcaagtcatc cacaaaaata
    25861 gtttcaggta caaaaagtgg atcttgtaaa attctcttcc aaacaatatt gtcaaaaccg
    25921 tccacaaagc ctgccttaag gagactcttg gtgtgaatga ttccaattac attgtcctta
    25981 tccccatcat aaaccgggat acgagaataa ttttgtttta aaatactttg gataatggct
    26041 tgactatcat cctgaatatc caccataaag gcatccgttc gaggcaccat aacctctcgt
    26101 gccatcagtt catcgagcga aaagacacct tgtagcatct caatctcatc agcatccaat
    26161 gtttcttcac tatttgtcag catataggca atttcatcac gggtcatctt ttcatccgca
    26221 tcatcgaatg acataggagt caaatggctc aagaaattgg tcgaagcagc taaaagccaa
    26281 acaaaaggac tgactagttt tccgatccca atgataatcg gcgctgtacg aattgccaag
    26341 gcatccttta gattaagagc gattctctta ggatataatt ccccaaaaac gatggaaata
    26401 taggtcaaaa atgccaagga tagaaaagtt gccacggctt gtgctgtttc gccattccca
    26461 agccaagagg caatcacacg tcctagagta tcagttaaac tcgcccctga taagattgta
    26521 atcagggtga ttcctacctg aatggttgat aaaaagtggt taggattttc tagtaccttc
    26581 agcaggcgga tgtagcgtct gtctccttct tccgcctttt gttcaactcg ggcacgatta
    26641 agagaaacca tggccatttc tgtggctgag aaaaaggcat ttaataccgt caagataaac
    26701 aataaaacaa attgtagcaa caaattctga ctacttgggt cttccatagt tcttctccta
    26761 aaatagtatc ttatccttta ttatatcaca aaatttaatc caatacatat tatttttttc
    26821 ttagcgtaac ttccatctct ctcccaaaac tggaagttag tctaagacgg atttccactg
    26881 atttcacgcg tttttctcat actaaaagct aggttgtggg aaactggaag ttagttttag
    26941 aagttactat tttttcttag tacctattct agccctgctt aaactagtaa aaagaactcc
    27001 tacatccata agtgacatag gagtttaggc ttatctttta ctgagttaca gttacttctc
    27061 cagttcggta atccagttga agtccctttt gaacattggg aactagaaca ttgaattcca
    27121 attctccatc cgaagacttg gcttcaatct gtgaagctga gggaactaaa tcctcgtttg
    27181 aagcgtagta aagggttaca cggtaacgga cacgcttgtt ttggtccaag gctttacgca
    27241 ccttgctttc atagtagttt tgaccagtcg aatactcggc ttgtgcctga tttgcccagg
    27301 ctgtctgaac agcaatgttt ttaggattgc ttgttgaggc atcaaaacca tccaaaccac
    27361 cgattaaggc atagcctaac aaatgacctc tatcgactgc atgggtataa gagcccttta
    27421 gattcttgac ctgatgccaa cctggaggag tccaagaagt tgaaccattc ccagtttctt
    27481 tacgattctt gtactgacga gtggccttag acaagagggc attagctacg gttggaacag
    27541 tttccttgcc cactgtcttt gttttattgt cagcgtaggg cttacttgaa accttggcat
    27601 ctagatttgt tttattacca ttgacgataa aagcacctga gccattccac tccagactcc
    27661 cctttatttg actcttgact gcgtctgtta agacactctc tgccaatgct tgactaggag
    27721 cttcagacgc ttgttttttc tgactaagat tggttttggg actattaggt gccgactgca
    27781 tctgcttgat ataatagctc cctgtagaca aaagcaataa cactagcagt ccgattagtg
    27841 tctgtcttgt ttttttgttc atatttctcc ttatctttag aaaaaggctg gttctccagc
    27901 ctaatttcct gtaaatttat gaatcaattc ctgccatttt gctggagaca ggatagccca
    27961 tggatcttga cccttgccca agattccata acctaccatt agaccgattc ctagagccag
    28021 agtacctaaa atcagtacta tgatgactaa aagtaaacga ttgattacgt agcttgattt
    28081 cttattcatc ttcatcgctt gcctcaatcc gctgaatctt agcacctagc tgcgccaact
    28141 tctcatggaa accgtagtaa cctctatcca agtgaaccaa tttaccgacc acagtttctc
    28201 cctgtgctac caaacctgtc aaaatcaagg ctgcactggc acgaaggtca gttgaaagaa
    28261 cttctgctcc ctgcaaaggc tgtccaccaa caatacgagc tgtatcacgg ataatctcag
    28321 aatgcaagcc catgcggcgc atctcttcta ggtgttggaa acgattttcg aaaactgtct
    28381 ccaccatggt tgattcgcct tttgcaactg tcatcaaggc tgtaaattga gcctgcatat
    28441 ctgttggaaa tcctgggtgg ggcaaggttt tcacatgaac agcttttaga ttttctagtt
    28501 gagaacgaac acgaattcct tcgtcttctt caattacttc aacacccatt tcaagtaact
    28561 tggcaatcaa gggacggttg tgctcccaga cagcgtctcg aatcaagaca tcaccaccag
    28621 tcatggcagc agctaccata aaggttcctg cttcgatacg gtcttggact acattgtgag
    28681 tcgtaccatg aagtttctca acaccagtaa tggttatagt ctctgtacca gcacctttga
    28741 ccttggctcc catttcatta aggagaatgg ctaagtcaac aatctcaggc tcacgcgcag
    28801 cattctcaat cactgtcacc ccatcagcca gagtcgctgc catcatcaag ttctgcgttg
    28861 caccaacact tggaaagtcc atatagatat gagcgccatg caagcgttct gccttggctt
    28921 cgatgtaacc agctgtctga ctaatcttaa cccccatagc ttccagacct ttcaaatgaa
    28981 gatcaatagg acggctacca atcgtacaac cacctggcat ggataccttg gcatgaccca
    29041 cacgggcaag gattggccct aatacaacga tggaggcgcg catcttgctg acatacttgt
    29101 aaggggcttc ctcagtgatg tcgccagtag catccacctt gacaagatga gcttcctcat
    29161 caaagtcaac cttggcattc aaaccaccaa ctacctgatt cataataaag acatccgaca
    29221 aaatcggaac attctgcaag acggtctttc cttcacttgc tagaatagtc gctgccaaca
    29281 agggtaagac tgcatttttt gctccctcga tcgtcacgct tcctaccaga cgattatcgc
    29341 caccttgaac cacaatttta tccatactcg tttcctttac tcttgatttt ataataatcc
    29401 tctaagcgct tcttgccaca aatgatacag gctaataaag aaagaactca ggatgtagcc
    29461 tatgacaatg ctaaaaaagg ctactaataa acgaactttt cttgtattat catctgtcac
    29521 ttttaaaacc ttctcccatc taacaagatc ctttaaaagg taaaaactca cataaataaa
    29581 gagcatatga ctacttagag tgaataataa ttgaaccatt tgactattat accatcttct
    29641 ctgtttgtgc gctagtttgg aaacttcact caaaaattag ggattgtttt tcaagtaatc
    29701 aatcgcatct tgtagggttt taacgggcac gattttcata tccgtcttga ttgttttagc
    29761 cgcttctagg gctgtttggt agttgttttt cgcgtccaga tgcgcctttt gttcttcttc
    29821 gctaacaggg ttatcaggag caaagaaaat agcagcacct gccctagccg aagctacaac
    29881 cttcttatca atacctccaa tgtctcccac attaccatcg cggtcaatgg tacctgtacc
    29941 ggcaacaata cgaccattac gaagatctgg gtgagctatt tgagtataga tagctagact
    30001 aaacatgaga ccagcacttg ggccgccaat accagctgtt gaaaagctaa ttgggacatt
    30061 gctgattacc tctgtacggt caatcaagcc gattccaatt ccatttttgc cattttccaa
    30121 ggtgataatc tttccttctg cagacttggt ttgcccatcc tcttcatagg tgaccttgac
    30181 ggaatcccct aatttttgag aactgacgta atcaatcaag tctttggaac tatcaaaggt
    30241 ctgatcattg actgctgtga ctgtatcaga gatattgaga atccctttaa aggttgaatt
    30301 atccgtcaca ttcaaaacat aaactccaaa gtacttgagt tcgatatcct taccagctgt
    30361 ttttagtcct tgatacttgg ccatattttg cgatgtttgc atgtagaatt gattgattcg
    30421 cataaattca acatcggaag aaccacctgt agtctcctga gcactacgaa tatctgtaaa
    30481 aggtgtcaac caagcataaa tcatatgagc taaagtggca tgttgaacac caaccgtaac
    30541 gaattgatag gcaccagctt ccttatcttc tgtgtcattt actttaagga cttggcgaat
    30601 atcttccgaa ccacctggaa cctctatata ataaggcaag ggcactacaa atgccaagaa
    30661 agtcacaatc aaggccgcaa tgacgtataa gggccatcta atcttttttt tcatttctta
    30721 tttcctccaa aatactctcg ggaacatggc aggcaatatc ctgaccaaac ttcaaaagct
    30781 ctcgaacacc tgatgaactg atatagagat gttcaggccg gctatgtaaa taaatagtct
    30841 ctatatcaga agacagctga tgattgtagt aatcaaaact agcttcatat tgcaaatccg
    30901 ccgcattcct caacccccgt actaggaaag tagcccccag tctttttgca acatcgacca
    30961 ccaattcatc atgagaagcc acgacttcaa cattttccaa atgtcccaaa gccttttcta
    31021 gcccccgttt acgattttca agagggagaa atccttgttt gtggggatta aaaaaaatac
    31081 ccacataaag cttatcaaaa agtctgctcg cccgttcaat gatatccaga tgcccatttg
    31141 tcatcggatc aaatgagcct gtgaataagc caatcttatc tgacatagac tgtcacctta
    31201 ctaattccat aaatcttttc cttccagata cccagacagg caatctcttc tggaagttca
    31261 acggccttat ccgtctcgca cacaaccata acatcttcag aaaaaagctc tctcttagcc
    31321 attttttcaa tatctgctac gatttgttcc ttggcatagg gagggtctaa gaaaacgagg
    31381 tcaaattccc cagatacctg ttccaatgcc ctctctgcat ccatcttgag gagttgaaat
    31441 tttccaactt ccttggtcat ctggatattt tcagccacga tggtctgagc cttacggtct
    31501 cgctccacca aaacagcact ggacatgcca cgcgatactg cttcgataga taaaccacca
    31561 ctacctgcat aaaggtccaa gactcgtccc ccttcaaagt agggaccaat catgttaaaa
    31621 atggctcccc taaccttatc cgaagtaggt cttgttgtct tgccttctag tgtcttgagg
    31681 ggacgtcccc catagattcc tgatacgatt ttcatactgt ttattatacc aaattataga
    31741 caaaaagaga aagaaaaccg aaccttgcgg ttcgattctc tacaaaatat tttcgtaagt
    31801 atcgcggact tcttgaggcc aaacacttgt ttgcacttct ccgatgtgtc tcttgcgaag
    31861 taggaacatg gccatacgag attgtccaat tcctccaccg attgtcaatg ggaataggcc
    31921 attcaacaaa gacttgtgcc attccaattc caagcggtct tcatcacctg taatttccac
    31981 ctgacgtcta agagtttctt catctacacg aattcccata gaagacaact caaaggctcc
    32041 acctaaagac tcattccaga caagaatatc accatttaga cccttgtagc cattctcaga
    32101 ctcgcttgtc cagtcatcat agtctggtgc acgtccatcg tgcggtttac catctggcaa
    32161 ctcgccaccg ataccaatca aaaagacggc tccaaattct ttacaaatcg cattttcacg
    32221 ttctttcggt gtcaagtctg ggtagcgttc taccaattct tctgtatgga taaaggtgat
    32281 ttgctttggc aagatagact cgatgtcata gcgagcttca acagctagct cagtcaggcg
    32341 aatagcctta taaatcttct caactgtttc ttttagataa acgatgttac gcttaccatt
    32401 tgggataacc ttctcccagt cccactggtc aacataaaca gagtgggttg catccaagga
    32461 atcctcatct ggacgaaggg ctttcatgtg gacaaagaga ccctctcctt caccaaagcc
    32521 aaaacgagcc aaggtgtggc gtttccattt agcaagtgag tgcaccactt cataagtagc
    32581 atcagggatt tggagaacct tgaccgatac tggattttcc acaccagaca ggttgtcctg
    32641 cattccgtca ccgaccttac tcaagatagg accttgaact tcgacaactt ctagcttatc
    32701 tttcaaatac tgggtaaaag tgtttttgac aaaggaaatt tcttcttgtt gatggataaa
    32761 acttttcttc atagacaact cctcaaaaat ttaataaacg tattatacac ctatttccaa
    32821 gaaaagaaaa gagaaaattt aaaaaaatca gtgtcactca aaacactgat ttcatagacc
    32881 ttttagtcat tgcgaccaaa gatacgtaga atacttagga aaagattgat aaaatcaaga
    32941 tagatgctga gagccattga caccacccag cctgtcgcta cacgaccttg tgattgttca
    33001 taggcgaggc gaatcttttg gttatcccaa gcaatcaacc ctgaaaaaac caaaaccatg
    33061 gctacgctaa tcatataatc aaagaagcca ctagccaaga aaatattgac taccatagca
    33121 atcagtaaac caatgagagc tgccatcatt gctcgaccaa tcccactcaa gtctttcttg
    33181 gtaaagatac caactgccgc catgacaaag aagagaaggg cgctcgatac aaaggcagat
    33241 aaaactgtac ccggagtata gaaagccacc acaaaactaa gggtaaagcc gtttaaaacg
    33301 gagtaaagta aaaatactgg aagagcagct ggactatttc ttgaagccat gctactggca
    33361 acgaaaacca aagccaactc cgcaaaagta gcaatggtca accagagacg cccctgcatc
    33421 aacaagtaaa ccaactgaga ctgaaagacc gtcaacataa ggcctgatac caaagcagat
    33481 agtccgattc ccagaccaac aaaggcataa accttagcgt aaaattgatt gagacctgca
    33541 cggtcatgta taatagtgtg attcatcctt ttctcctttt ctgtgaacat ctttccttga
    33601 ttataacaaa aaaagttaaa attagcttaa atggaagatt aaaataccgg ctgcaactgc
    33661 aacattcaaa ctctcggcct gccccttcat gctaatatgg accaactggt ctgcactttc
    33721 agccataagg ggactaattc cttgaccctc atttcccata actagtacaa aattttctat
    33781 agaaggcagt tctctgtaat caacagaatc tttagatagg gttgttgcta gcactgggat
    33841 agctaccttt ttagtttcgt caagaagcgc tggactagtc atccggtaaa tgggcagatg
    33901 gaaatgacta ccttgcatgg aacgtagggt tttgagactg tagatgtctg ccgacttatc
    33961 tgaaacaatc actcctgtaa aacctgctgc atccgcagtt cgaatgatag ttcctacatt
    34021 accgggatct tgcacatctt ccaaaaacaa gaacttgccc tgactcaaat cagcttgtcc
    34081 tacttcttct ttttgaacca ccgccacaat tccctgtgga gtctgagaat ccgccaaatc
    34141 tagcaaaata tcctctgaca cccagacagt ttgcgaaaaa gtagctaact gatctcgata
    34201 attttctagg gcaaagattt tctcaatcgt cactccagct tggacagctt cttcaaacaa
    34261 gtgccagcct tcaatcaaat aggcagactt gcggtatttt ttttggcgta atttcttggc
    34321 attttttacc acagaattgg cttttgaggt tataatagtc atagaaatat tataacacaa
    34381 tcaaaggggg ttggtatgca aaaggttaga atgattgccc aaggtagggt acagggagtc
    34441 ggctttcgtt ggggtgttta cagcttggca cttgaaattg gtggcatcac aggtcgagta
    34501 tggaataacg acgatggcac agtggaaatc ttagcccaag cagactcatc tgctatcatg
    34561 gcaaaattta tccaagaaat ccgaaaagga ccgacacctt tttcaaaagt aagctactta
    34621 gatgtcaaac taagcaactt tcctccctac tctgacttta aaatcgcaaa ttaggtctcc
    34681 agaactattg tatattttgt aaaaaaacag tagaatagaa aggtataatt tttaaagaag
    34741 gaataaaaac agtgaaatct attaaacgtt ttgcattctc ggctatggga gtggctatgt
    34801 tgcttgtctt gactggctgt gtcaatgtcg ataaaaccac aggtcagcca acaggattta
    34861 tttggaatac gatcggagcg cctatggctg aagccatcaa gtacttcgct actgataaag
    34921 gtctaggctt tggtgtcgct atcattatcg taaccattat cgtacgcttg attatcttac
    34981 cacttggtat ctaccaatca tggaaggcaa cgcttcactc tgaaaagatg aacgctctca
    35041 agcatgtcct tgagccacac caaacacgtc tcaaagaagc aactactcaa gaagaaaaac
    35101 tcgaagccca acaagccctc tttgccgctc aaaaagagca cggcatcagc atgtttggtg
    35161 gtgtaggatg tttccctatc ctccttcaaa tgcctttctt ctctgctatc tactttgctg
    35221 cccaacatac tgaaggggtt gctcaagcaa gctacctagg cattcctcta ggttctccaa
    35281 gtatgatttt ggttgcctgt gctggtgtcc tttactatct tcaatcgctc ctttcacttc
    35341 acggagtaga agatgaaatg caaagagaac aaatcaagaa aatgatttac atgagcccac
    35401 tcatgatcgt cgtcttctcc ctcttctcac cagctagtgt cacactttac tgggttgtcg
    35461 gtggtttcat gatgattctc caacagttta tcgtcaacta tatcgttcgt ccaaaacttc
    35521 gcaaaaaagt ccgtgaagaa ctagccaaga acccaccaaa agcaagtgct ttctctaaac
    35581 caagtggacg aaaagacgtt acccctgaac aaccaactgc tatcacaagc aagaaaaaac
    35641 acaaaaatcg caacgctgga aaacaacgtt cgagataaga agaaaaaggc ttagctgatt
    35701 tgctaagtct ttttgcaaat caaaagcccg atgtttccat caggcttctt ttctatccat
    35761 gtacacgttt catataatct tgataacttt cggtatccat gagtttttta gcgttcttga
    35821 cgcgatctgc tgttggtggt ttgactcctt cgagggaata tggaattcca agttcacgcc
    35881 acttgaactc acccatggtg tgataaggta gaatttcaaa cttatcaaca tttttgaggg
    35941 tcttgacgaa cttaccaagt tcaatcaagt catcatctct gtctgtcaat cctggaacta
    36001 gcacgtggcg aatccagaca ggttttccaa tatctgatag atactgggca caagccaaga
    36061 tatttttatt agtttggcta gtgacaatct tgtgctgttc ttcgttgatt tccttgatat
    36121 ccaaaagaac caagtcagtg acagccatga gtttgtcaaa cttctcaagg taacgtggtt
    36181 tattacggaa aggaagagca caggtatcca aggtacagtg gattccttgt tccttagcct
    36241 tggtgaagag agcaatcagg aaatcaatct gcaagagagc ttctcctcca ctgactgtaa
    36301 tcccaccctt atttccccag aaaccacggt agcgcaaggc ctctgtcaag acatcatcta
    36361 ccgtccgttc acgtgactta ttggactcca tagcccaagt gtctgggttg tggcaatact
    36421 ggcaacgcat gtgacagccc tgcaaaaaga caataaagcg aataccaggc ccatctactg
    36481 acccaaagct ttctgtcgaa tgcaccattc ctgtcacttg tccataatca attgtttctt
    36541 cagacatatc gttaccttcc ttgaaaacgt tttatagttt tattatatca cgacttgaag
    36601 gaaaaacaag atttttgcct attttttaga aagcaatctg tttttcaatt tcattttcaa
    36661 tctattatca gattattctt caaaatcgaa gccatataag gttgcaaaat agtcctcagg
    36721 gcgctctgca cggcggattt gacgggcttt accttcttcg gtatagagga tttccgcaga
    36781 acgtaatttg gcattatact ggtagcccat tgaaaatccg tgggcacctg tatcatgaat
    36841 taccagcaaa tcaccgattt ctgtatgagg cagttcgcga ttgactgcaa atttatcatt
    36901 gttttcacag agtgaaccaa ccacatctac cgcttcagct ggtccatctg gatgggtcac
    36961 gttgctaata tgatggtaag ctccgtacat agctggacgc atgaggttga ctgctgaggc
    37021 atccacacct agataggtac ggtaggtttc cttcttatga gtcactcttg tgactagagc
    37081 accgtgaggt gccagcataa aacgacccaa ttcggtgaaa atcttgacct gaccaagacc
    37141 tgctgacgta agaacttctt catacacctt acgaactccc tcaccaatca aggcgatatc
    37201 gttcggctcc tggtctggac gataattaac accaataccg ccagaaagat tgataaagtc
    37261 tagcgaaatg cccaactttt ccttgatttc aacagccagt tcaaagagct gacgagccaa
    37321 ctctggataa tagagatggg tcacggtatt ggacgctagg aaggagtgaa tcccaaaagt
    37381 cttggctcct ttttccttca agatagcaaa ggcttcaaag agctggtcct tggtcatgcc
    37441 aaacttagcc tccccaggat tgtccataat gtctgtcccc agttcaaaaa cgcctccagg
    37501 attataacga caagagatga tttctggaat gcctgctact ctctccagat gttcaatatc
    37561 ttcaaaggca tccaagttaa tggtcgcacc caattcacgt gcataggcgt attccttgtc
    37621 tggcgtgttg ttggaagaga acataatctc agaacccaga aagtccagtt tatggctcat
    37681 caaaagctct acataactag agcagtccac accacaacct tcttcttgga gaattttcaa
    37741 aatagctgga gttggagtag ccttaactgc aaaatattcc ttaaagccct tgttccacga
    37801 aaaagcttgg ttgacggctc ttgccttctc acgaatcccc ttctcatcat acaagtgaaa
    37861 gggagtcggg aactcggcaa caatcgcttc taactcttct ctattgataa atggtgtttt
    37921 cataagcttc cttctattct gtttgcaaag aaaggctggg acaatcgttc caacctttag
    37981 ttctatctct tatttatctt cgtcaaagat attgccaacc tcaacatcga tggcttggtt
    38041 cttgacatca atattggtta ccttcaagag tgacttgtag tcaaactgct tttcacgttc
    38101 ttcttgggca ttgtccgcaa agaccgctac acctgccagt tctgagtcga actcgcgcaa
    38161 gagactaatc ataccattga ccgttccgcc acctttcaag aagtcatcca caatcaagac
    38221 acggctgcct gccttaagac tacgttttga aaggaacatt ttctcgatac ggtcaccact
    38281 tgaacctgaa acatagttga cgctaacagt tgaaccttcg gtaattttca ggtcacggcg
    38341 cacaatgaca aaagagacat tgaggacatt ggcaactgca tttgcaagtg gcacaccctt
    38401 agttgctacg gtcataaccg cgtcaatttt ttggtccata aagcttttgg caataatacg
    38461 accaatattt ttcaagatgg ctggtgtgct aagcaaatca gacagataga tataaccacc
    38521 tggcaagata cggtcacttt ctgacaactt ggtacgcaag tcttcaacca tttccttagc
    38581 atcctgactc gaaatagacg gtgtgaagat gacacctccg ccagccccag tcactgtctg
    38641 gatatgaccg atttcaattt cctcaaaggc gcgtttgata atgacgatat cttctgagat
    38701 ggatgattta gcagactcat acttttcagc aaaagtattg agactagtta gtttataagg
    38761 attattaatc aaatagttgg aaatgacaac catccgatca cttcttctta atttcatttc
    38821 tatttcctat tccattttta ttcaaaaaat caaaaagcaa actagaaagc tggtcgctgg
    38881 tggttcaaaa cactgttttg agattgtcaa tagaactgac aaaccctgta atatacctgc
    38941 atatatacat acgacaaggc gatactaccc tagtttgaag agattttcga agagtattca
    39001 tttttgtctt ttacttatta taccatattc acataaaaaa acgaacattc ttatcctaaa
    39061 aaatgctcat ttttcttaaa ttatcaatct aaatctggtt tatagaagga acgattatcc
    39121 atagcgaaga ttttattggt catctctcct ttatccacca aagccagagc tgttgacatc
    39181 atcatcatgc ttgcatccag attgtcaatc atatggataa tctctgcctc cataatacgt
    39241 ggacggactg gacttccata ttcaagcaag ccgtggtgac tgaggatgac atgacgaagc
    39301 aaaacgactt cttccttggt atcatcgatg ccgagttcca taactgtctt ggtaatttcg
    39361 ctatcaatga gagcgatatg tccaagaaga ttacctcgca ctgtgtactc tgtctggtct
    39421 ggccccgtca actcgataac cttagctaag tcatgcagca taatccccgc atagagcagg
    39481 ctcttattga gctgaggata aacttcgcta atagcgtctg ccaaacgtac catggtcgcc
    39541 gtatgataag ccaaccccgt ttcaaaggca tggtggttgg tcttggcagc tggataggag
    39601 tagaattcct tatcatactt ggtgtagaga tttcggacaa tccgttgcca gacaggattt
    39661 tcaattttga aaatcatttg cgacatgtag tcacgaattt ccttgacatc aactggtgac
    39721 ttgaccttga aatcggctgg gtcattgggt tcaccagctt gaggcaggcg gagagtaatt
    39781 tgattgactt gaggggtatt gttataaact tctcggcgtc ctttcatgtg gacaacctta
    39841 cctgcggtaa aggcctcaat gttatgaggt tgggcatccc agagcttccc atcaatctcg
    39901 ccactatcat cttggaaggt aaaggctagg tagtttttcc cagctcgagt ttgcctcagg
    39961 tcagctgatt tgattaggta aaagccttca aatagctcat cttttttcat gtgactaatc
    40021 ttcatattct tcctcatttt cttgaaaatg gagtagatca agcgcaggct caccttctga
    40081 caactcaatg tgacggagcg tccgctcgat agctatggta cgacggttta ataattcatc
    40141 aatattgcca gaggcatgtt ggagatgttt ttgtgccttg accagaatac caccaaactt
    40201 gccaaactcg gtcttgacac tggcaagagt cttgctgata tggtcggcac tcttttggat
    40261 attaagggtc ttgaaaccaa ctgatagaga gttaagaagg gctgataggg tacttggtcc
    40321 tgcaacaata atctgttctt cccgtctcaa atcatcaaag aagaccggat tgcgcacaat
    40381 ctctgaataa agaccttctg tcggaacaaa caagattcca aagttggttg tccgaggtgg
    40441 tgctatgtac ttgttcctaa tatccctagc aaagcgcttg acgcttgcta ggagtgactt
    40501 acgacagcgt tcaatctcat ccttgtcacc tgtctcatag gcttcttcca agcggtaata
    40561 atctgccagt ggaaacttag agtcaattgg cagatagacg tattcttggt cgccttgtcc
    40621 gggtaacttg atggcatact ccactcgttc actagagttt tcaaccgttg cgtattctcg
    40681 ttcgtactgg gcaggtgtca tgatgtcttc aataatttgc cccagttgca attctcccag
    40741 aatccctcgc gtcttggttc cagagagaac cttgttaaga gctccgacat cacgggcaac
    40801 tgtctgcatt tctccaaggc cacgattgac agactccagt tgtttagaaa ctgtctcaaa
    40861 ggaagcctgt aagcgtgtct gcaaggtctt ttctagtttt tcctcgaccg tctggcgcat
    40921 ttgttccaaa cgttgctcat ttgattcctg caaggcttgg agacgttggt ctgtcttgtc
    40981 tctagtttgg aggagattat ctgtcatttc ttgacggact tgggtcagac cttggtgcaa
    41041 ttcaatccgc acttcttgca aacggtcgct gacaaccact tccaaatctt tttggtctaa
    41101 ctggctggct tgtctggctt ggtcaaagcg gtaatccaac tggtctgaca aatgatctgc
    41161 ctgatcctcc aagctcttac ttaagtgttt ctcctgccta tcctgccttt gccaaatcag
    41221 aaagagacca gctagattgg caattaataa tagaataagt aaactttcca tcctacctcc
    41281 tgtccttgct atgcagtacg accacataac catctgggca agttaccgac acttccctat
    41341 ctatatattc gttagaagcg tacacttttt taaagaaaaa attttcctct gtcaactcat
    41401 acttggctcc aagaatagtc agctggctat cccgaactgg cataaaggct agatagtcgt
    41461 agtctgaacg aggttctagc tgactgattc cttctggaca ataagtaatc aagttttgcc
    41521 catcctcaat ttctatttga tgcatatagg gtgccaactt aggattgcta ggcagaaaga
    41581 cattggccaa catatggtca atacggccac ccaaggcacc gaaaatagta acctgagcct
    41641 gaggattttg cttaaagatg gtcaagagag ccaattccaa atcagtatca tctttttctg
    41701 gccgggcttg gacaaaatac tgggcacgtt tttgaatcac ctgtcgctct tcttccgtca
    41761 cagaatcaaa atctccgact gctagagcaa gaggcaaatc ttcttccaag atccagagcg
    41821 agcctcgatc cacaccaaca aaaccatcaa agtccgtccg ataatgaccg cggtctccgc
    41881 ctgcaaaaac ggcaacccta gtccagttgt tttctgagag tttgtactcg ctcattgaca
    41941 tctcccttaa agacatagga acctgctaca aaaacggtcg caccagcttc tttggattga
    42001 gcaatagttt ggtcatcaat cccaccatcc acttcaatct caaagttcaa acctttttca
    42061 tcacgaaggg caaccagctc acgaacctta tccatggttt ctggcagaaa ggcttgacca
    42121 ccaaatccag ggttaactgt catgactagg acttggtcaa ctagatgaag cacatgctta
    42181 atcgcttcaa ctggtgtacc aggattgata acaactgatg gcttgacacc gagtgaacga
    42241 atcttttgga gggcaccatg aatatgaggc gttgcttcca catggatact aataatatcc
    42301 gcacctgcac gcacaaagtc ctctaaatga tgctcagggt ttgacaccat caagtggcag
    42361 tcgaaaacca tcttactatg aggacgaaga gcttcgacca cacccgcacc aaaactgatt
    42421 tgcggcacaa aatgaccatc cataatatcg atatgggcat actctgcccc agttgcttct
    42481 aggcgtttaa tttcacgttc aaagttggca taatctgctg ccagaattga cggagcaatc
    42541 ttgtattgag acataggttt ctccttattt tggaattttt ttgctgactt ttttataggt
    42601 ttctctgcga ttttcaattt cgctgaggaa ttgcaggtag ttgtcaaaac ggaagggtgc
    42661 aatggtacct tcttcaactg ctggcttgac agcacaggac ggctcatggg tatgggtaca
    42721 agtacggaat ttgcagtctc gactgacagt ggcaatctct ggaaaggcct gattgaggtc
    42781 ttcagccgtt gatacttcat aatccagtga tgaaaagcct ggtgtgtctg cgattttacc
    42841 cccattgagg ttgtaaaaac taacagctcg agtggtatga cgcccgcgac ccagactgtc
    42901 tgaaatttct cccgtttcaa gattaagatc tggtgcgatt ttattgagaa gagttgattt
    42961 cccaacaccc gtctgcccca taaagaccgt aaccttgcct gttaacaaag gcaggagttc
    43021 ctctttactg gtcacaaagt catatccgat gtcaccatag gtctgctggt aaaaatccag
    43081 ttctcccccg tcttccaaca aatccatttt agaaatatag acgatgggat gaataccctt
    43141 atgctccaaa agaaccaaga aacgatccag caaattgctg ttaaaatcag gttccttgac
    43201 agacatgatt actacagctt ggtcgatatt gacaataggc ggacggacca gactgttttt
    43261 ccgttcgtga atcttgagaa tatagccttc tgaattttcc tcggcagaga aatctaccca
    43321 gtccccaacg taaggggtat gccctttttt acggaaattc ccacgcgcgc gtgtttgata
    43381 gacttggcca tcactctcta catagtagaa cccagccaag gctttaatga tttgtccctg
    43441 caaaataact cctttaaaat tgttagttcc attataacaa aaaaaagcta ttttagctta
    43501 gcttctcttt acaatttcga taagtctatt ctaaaagaaa aagatacact cacgctttag
    43561 ataccaagtt ttatacaaaa ataacgaatc tacctcctta tataaaaaca ggcgaacaga
    43621 gagcctaaaa ctccctgttc gctttacttt ttctgtgtgt aatagaaaaa acctaataaa
    43681 gcaagacaaa aaatatgaat ataaactcca tacatcatgt ggggaaagac agactctata
    43741 aaatagcctg gtagatagat gattggaaat tgatagaaat cataggtcgt tagagatagt
    43801 aaacaaagaa catacataaa tactgctaca attagaaccc atccttgctt tcgccataga
    43861 aaaatcatca ataagaacag acaaagtaga tagattgacc aatttcgcaa cataaattga
    43921 aaaccataaa tcatactttc agtcgtttct ccatcaatca catcaactac tttatagaaa
    43981 ggtaaacaat agctcactaa aaaaagaagg ggactaatta tttttttcat attctctacc
    44041 atggattatt ttgcaagtaa gctgaccatc tatctccata tcgaatcaaa ttaacagtat
    44101 aattagttac tacccgattt tcacctggtc caaccttaga aaaacctgtg ctatactccc
    44161 aactacttct tgcacctgga tgaaaccatt tttgctggga tgaataccaa ccaccttgtc
    44221 taacttccac acttgaagat tctatttcga tatccctttc atggaagtca actcctccac
    44281 ttattttagt aatatttttt tgtacacctc catcataact cacccagtga acaacagtat
    44341 acaattccac actataagta ctatcaaact gactttgatg agtagtaatg ttttgagtat
    44401 aagcgtgcac tttctgtata ccaaaaatat ggtctaatat attcgttggt ttagcaagct
    44461 tattgtaacc ctcttcaaca aaagtagcct tctctaaatc agcggtataa acagtttttc
    44521 cttgtactac ttgattgttt atagaagtaa tacggctatc cacaacaatg ggggccacca
    44581 tatctccatc gctagtttct attttagctg cctctttcac ctcatataca actcgatttt
    44641 cttcagactg aatcacttct accccatttt tttcaggtat agataatcta gtttgcccat
    44701 tagaaaccgt tatatttgga ccaatattct tctcatttgc ataagtcgca ataggaaatc
    44761 ctgatagaac cactgcacac ataaggctat tgactaattt ttttctcatg aatatatcct
    44821 tcccattttc aagaggagta aaaatagcta atataagatg aacgtttcct tatatttcag
    44881 aataggatat ctatctgaaa gcaattcaca ctcctatagg taactgttcc aaatcttggc
    44941 tacatatatt tcaatagttt gctacaaaaa actcctttct aagtctattg aacatgccct
    45001 tattcaccta ctaatatatc acaaaaataa ataaatacaa gactgttttt tctactagtt
    45061 ttatgataga atagtattgt tagggtaaat ttcatatata aatgtcgagt gaaaaatcga
    45121 tggagcagaa atcggtagac aaagactcga tttcgttggt ccagccccac aaggctaatc
    45181 aggtatttca cgagtttaaa aatctaatga agtgcccaat caactactga ttcattgatt
    45241 taatacgaac tatagtggat tgaacctaga aatagtacac taaggattct aaaacgtttc
    45301 tagaaattaa tttgactttc ctaatctctt tgttcatatc ttatttcaat ctactatata
    45361 taccaaaatg ctgggacaaa agtctttaaa atcagagaaa cgcatattat caggtgttgg
    45421 aaaaccttga taatatgcgt tttatcatag aaagatctac ttcatttttt accaaaatta
    45481 agtttttgtc cctgcatctt agagtccttg ccctttaagt gcatctgcta ggctggcaaa
    45541 ctcttccaag ctgagagctt ccccacgcac acttggtgac aagcctgctt ggtccaaagc
    45601 cttggtcagc ttgtccttga cctcttcagt cttgccaaag taacctgtca agttattcca
    45661 caaggtcttg cggcgatggg taaaactagc cttggaaacc ttaaagaaaa agttctcgtc
    45721 ttccactgct acagctggca ctggacggcg caccattttc aagatggctg agtctacatt
    45781 tggtgctggc acaaagactg tacgcggcac gataaaggca accttggctg tcatgtaata
    45841 ctgcacagcg atagacaagc taccgtaagc cttggtattt ggttgagccg agatacggtc
    45901 cgccacttct ttttgcatca tgaccacaaa ctcactaaaa ggaatgccac tctcgattaa
    45961 gtgcatgaga ataggcgttg tgatgtagta aggcaaatta gccactacct tgattggcag
    46021 gtcaggattt ttgaaattct ggatatgttg cgccaaatcg accttgagaa tgtcctcgtt
    46081 gactacagtc acattgtcaa aatcacgcaa ggtatctgcc aaaatcggta ccaaacgatg
    46141 gtcgatctca aaggccataa cttctgctgc acgctcagcc aaaaactcgg tcaaggcacc
    46201 aatacctggc ccgatttcaa tgacattgac ctggtcatca atctcagccg tatccacgat
    46261 tttttgtagg atattggtat ccgtcaagaa attttgccca aaggactttt taaaggtaaa
    46321 accgtgacgc tccagcactg ccttggtcac gctataatct gcaattctca tctattctct
    46381 tttctattat ctgttactac tattgtaaca cattcccctt gtattttggg ggtgaaaatc
    46441 ataccaaaaa actatctttc ctcatgaaga aagatagtct tggaaatctt taaaagtggc
    46501 cttagcgctt ctctcgaaac atacgtt
//
LOCUS       DAVBNO010000011        44376 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVBNO010000011 DAVBNO010000000
VERSION     DAVBNO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.011"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..207
                     /locus_tag="VQ846_000650"
     CDS             <1..207
                     /locus_tag="VQ846_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3075282.1"
                     /translation="MDNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIK
                     KHLKKVLPSCNTFYEAFLSCSCFN"
     gene            complement(242..1039)
                     /locus_tag="VQ846_000651"
                     /pseudo
     CDS             complement(242..1039)
                     /locus_tag="VQ846_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1304..1540
                     /locus_tag="VQ846_000652"
     CDS             1304..1540
                     /locus_tag="VQ846_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET3075283.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            1771..3057
                     /locus_tag="VQ846_000653"
     CDS             1771..3057
                     /locus_tag="VQ846_000653"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET3075284.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(3289..4437)
                     /locus_tag="VQ846_000654"
     CDS             complement(3289..4437)
                     /locus_tag="VQ846_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET3075285.1"
                     /translation="MKYNKTKYPNIYYYETAKGKRYYVRRSFFFRGKKREKSKSGFTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKAKFGSILLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATGLHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            complement(4612..5424)
                     /locus_tag="VQ846_000655"
     CDS             complement(4612..5424)
                     /locus_tag="VQ846_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HET3075286.1"
                     /translation="MQKTVEKILFRVAGVTKYKKAVKEACNMIAEDNGIPEYAKYYSN
                     LSTKELREELEEYGLKVFKYQDLDFFNIELVPEVDNRYDPNAIKVLIFDNHVGYVPAT
                     IAKTIRKYFDNKKYNFVIEGEIKGGPYKEWDEYEEKVVTNNDLDVGFEIYLTIVDSSQ
                     KEVIQSESSEIIDDNISNKEVTETEAIETKTTETEHIEQNIVSDSVADIVNEINLSET
                     SPKKKLPANKIIFSALYLFLVFFGVVGIPIAPFLAVPLTAWSLYKLYKLFRK"
     gene            complement(5462..5842)
                     /locus_tag="VQ846_000656"
                     /pseudo
     CDS             complement(5462..5842)
                     /locus_tag="VQ846_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
     gene            complement(5855..6118)
                     /locus_tag="VQ846_000657"
     CDS             complement(5855..6118)
                     /locus_tag="VQ846_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET3075287.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            complement(6118..6351)
                     /locus_tag="VQ846_000658"
     CDS             complement(6118..6351)
                     /locus_tag="VQ846_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075288.1"
                     /translation="MTITINFTEKNSYITDYLTKHGIDTTTMDFDDFMVLMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLSREDIA"
     gene            complement(6351..6719)
                     /locus_tag="VQ846_000659"
     CDS             complement(6351..6719)
                     /locus_tag="VQ846_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3075289.1"
                     /translation="MFVAFDKIKELADKQGISINILEEKLGYGTNTLYRLKRSNPSSK
                     VLKEIADYFNVSADYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            7004..7150
                     /locus_tag="VQ846_000660"
     CDS             7004..7150
                     /locus_tag="VQ846_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075290.1"
                     /translation="MNEEDLKELLELLATDYGRGYLDGVVGGLSMLLKISKEAEKYRK
                     EKYE"
     gene            7194..7568
                     /locus_tag="VQ846_000661"
     CDS             7194..7568
                     /locus_tag="VQ846_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075291.1"
                     /translation="MIETLEIKINKKTYFISARADVPVRYKVMFGRNLFDDFSKIKNN
                     KDETLSLCLLFCMTDKSSEEFCKFFIENNSYITSRHHYEAIRLFEKHFIFKPVTYSLE
                     DIVQILTIYLENTKTQIGGKNE"
     gene            7561..7764
                     /locus_tag="VQ846_000662"
     CDS             7561..7764
                     /locus_tag="VQ846_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET3075292.1"
                     /translation="MSKELKIIKAKIKTRLIELDMTQVELAKQVSVASSVISELLKYG
                     KGSDYVKEKVVDILGIENPWENS"
     gene            7781..7978
                     /locus_tag="VQ846_000663"
     CDS             7781..7978
                     /locus_tag="VQ846_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075293.1"
                     /translation="MNNAAQKVTRIDKDAWEIATELANEYGVSICHIISESVRYCAEN
                     AEFKEMDVVVKRLVVGSKVLE"
     gene            7989..8150
                     /locus_tag="VQ846_000664"
     CDS             7989..8150
                     /locus_tag="VQ846_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075294.1"
                     /translation="MNELVWFYFVVIINLIIGFATYYASKRDREKRINGFKKIQDDEL
                     ERVRNKFNL"
     gene            complement(8145..8570)
                     /locus_tag="VQ846_000665"
     CDS             complement(8145..8570)
                     /locus_tag="VQ846_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075295.1"
                     /translation="MEYQALIQPVISIILAIISGLWSYVASKANNKAEIEKQAKEHSH
                     TVEKLEREFHHQIDTLKQQHAFELEKIKQAHELRLQELEKVSQLDTETDKSMKMNDLT
                     YKVFTGEVDFDKALKIANKASNSKQNLNKRFIQKTSKKS"
     gene            8624..9337
                     /locus_tag="VQ846_000666"
     CDS             8624..9337
                     /locus_tag="VQ846_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HET3075296.1"
                     /translation="MNEIFNFHGQEVRTLIIDDEPWFVGKDVADILGYSKARNAIALH
                     VDEDDALKQGLTDNLGRVQETIIINESGLYSLILSSKLPQAKEFKRWVTSEVLPAIRK
                     QGGFIREDLDEDAFIALFTGQKKLREQQATMLEDIDYLKSEQPIHPSYAKSLLKKRKA
                     RVVVCLGGIDSPAYADKTFAQSVFRQAEIDFKDHFNINRYDLLPKKFADAALAYWMTW
                     EPSTNTKMKIMKLNSFDEG"
     gene            9350..9607
                     /locus_tag="VQ846_000667"
     CDS             9350..9607
                     /locus_tag="VQ846_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET3075297.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCD
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            9693..10013
                     /locus_tag="VQ846_000668"
     CDS             9693..10013
                     /locus_tag="VQ846_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075298.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEQRKEEERREEQEF
                     ASMIIQVRNHAYERGREDKWQEIRKNIRREFKGFTYDNEPPVGLRPEPLALPEPKQSA
                     IRFL"
     gene            10029..10325
                     /locus_tag="VQ846_000669"
     CDS             10029..10325
                     /locus_tag="VQ846_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075299.1"
                     /translation="MQELIESLDNLIMIVKELEGRESTSRHFITIWENDYKNLLRVKE
                     YLTDYEKLAKDYRYMTLKNKLLKIEKMELEGRYIYEDMRMKYRANRRKWGARYV"
     gene            10318..11145
                     /locus_tag="VQ846_000670"
     CDS             10318..11145
                     /locus_tag="VQ846_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HET3075300.1"
                     /translation="MSEIKWIKIKTDIFDDEKMCLIDASPDRDAIIVIWIKLITLAGK
                     LNTKGVLTISKNIVYTDEMLAQTFHRPLNTVRMALEVFEKFGMVEKIDGVIMLPNWEK
                     HQNIDGMEKIKEQNRNRAARHRQKQKLLAQNNESNVTDNVMDNVTVTHGNALDKELDK
                     DIEINNNKVMISSSLSENLKNSGIHLTDKSHQQLLDYVGLDGMSFDMLNRAVEKTSGS
                     HKPSFNYLIAILESWKKKGFTSIEQVDEDDRKYKEGKKSRQQNDKTSEQEVRDEWGF"
     gene            11285..12055
                     /locus_tag="VQ846_000671"
     CDS             11285..12055
                     /locus_tag="VQ846_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET3075301.1"
                     /translation="MVDNVFEEIALSYRRNTEQQEEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQERVNELANAENERERKYYLEKFSLYDEILKNATLDNFETPTEKEAEK
                     LAFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLIS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            12070..12264
                     /locus_tag="VQ846_000672"
     CDS             12070..12264
                     /locus_tag="VQ846_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075302.1"
                     /translation="MFILKHGTREDKPFLRSAIISVTGLDISCSEEKKAMRFISRAAA
                     LQVSKALRGSFGNFYPVEVE"
     gene            12264..12491
                     /locus_tag="VQ846_000673"
     CDS             12264..12491
                     /locus_tag="VQ846_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075303.1"
                     /translation="MLELYFVYNGHCKFFLGRFDNVDDLIEQMEDHQWAFSAITHPRF
                     QKHIGQRTTRFDYGSKDCYYLATFSGGEKND"
     gene            12618..12785
                     /locus_tag="VQ846_000674"
     CDS             12618..12785
                     /locus_tag="VQ846_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075304.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEVR"
     gene            12772..12981
                     /locus_tag="VQ846_000675"
     CDS             12772..12981
                     /locus_tag="VQ846_000675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075305.1"
                     /translation="MKYDKQAEIDGLKRTIEQNEEKIIEYSKPCDARKRRIRALERDL
                     LRKKNKELRRKAEELEYDGRVKAKS"
     gene            12953..13282
                     /locus_tag="VQ846_000676"
     CDS             12953..13282
                     /locus_tag="VQ846_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075306.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWVGYSPSMSQLVIAVYLIY
                     RGSEDET"
     gene            13272..13697
                     /locus_tag="VQ846_000677"
     CDS             13272..13697
                     /locus_tag="VQ846_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HET3075307.1"
                     /translation="MKLNELIKKYKKLEGVWNAEGAELVRQIFLQDLEQLDKPQPVKV
                     PQCVAEYIEFKKKNNFHVYGLAWLNGYEVEKEKRYFVKIKGNIKENMLVYGELLKRYF
                     FTKSFSLDDVIYSHTRKELEAANFGWVFDCPGIEIEEVE"
     gene            13697..14211
                     /locus_tag="VQ846_000678"
                     /pseudo
     CDS             13697..14211
                     /locus_tag="VQ846_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502465.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            14208..14597
                     /locus_tag="VQ846_000679"
     CDS             14208..14597
                     /locus_tag="VQ846_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075308.1"
                     /translation="MIPKFRVWHHELGRLMSVKCMFFQDSEIEEFELNDALMNDYITA
                     YPDEIELMQSTGLKDKNGKEVFIGDIVKCTRGCLHEVYLEKEYGGTFIGGMPAVYLKG
                     LGDGYAWTEYEEIIGNIYENPELLEDK"
     gene            14597..14833
                     /locus_tag="VQ846_000680"
     CDS             14597..14833
                     /locus_tag="VQ846_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HET3075309.1"
                     /translation="MNPEIIDNINKPSHYQGANGLEAIDVVHNFVGSLSGASAFFWGN
                     AIKYMLRFQKKNGLEDLKKARKNLDWLIEEMEHE"
     gene            14830..14988
                     /locus_tag="VQ846_000681"
     CDS             14830..14988
                     /locus_tag="VQ846_000681"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075310.1"
                     /translation="MNTHCMKATPLLPWGRLRKSAKKQELLNGASISEFAIEDICTFF
                     GIEIQERK"
     gene            15200..15601
                     /locus_tag="VQ846_000682"
     CDS             15200..15601
                     /locus_tag="VQ846_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET3075311.1"
                     /translation="MKKLSDEELKTLDRELFKFQNIQRTIDLRRLELETRNPDAQSGP
                     TVGISKPTETIAIRIADDPTLKFLEGFKGIINKLLSNLVEEDKEIFNLRWRYPQLRWE
                     EIAEQKFMSKATIYRRRRIILEQYAIMKGEL"
     gene            15790..16332
                     /locus_tag="VQ846_000683"
     CDS             15790..16332
                     /locus_tag="VQ846_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET3075312.1"
                     /translation="MEFVSPIKDNDNIQAMKDYLREWNEMYYMLFITGLNTGLRVGDI
                     LTLKVKDVQGWHIKLRERKTGKQITRRMTKELKKEMRRYVEGKPFHHFLFKSRQGQNK
                     AITRERAYQIIHEAAEELGIDNVGTHTMRKTFGYKYYNKTKDVGTLQKMFNHSSPAIT
                     LRYIGIEQAELDDALRNFVI"
     gene            16496..16687
                     /locus_tag="VQ846_000684"
     CDS             16496..16687
                     /locus_tag="VQ846_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075313.1"
                     /translation="MVKEYRDDFLGEKAFEKLNKDIDANPEVGFEIVGYTQTAFVNGM
                     HIPLTAILVKWNNFFKESE"
     gene            16807..17127
                     /locus_tag="VQ846_000685"
     CDS             16807..17127
                     /locus_tag="VQ846_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET3075314.1"
                     /translation="MYNKPIRPSLKSKKWEKFRDRIMRKHDYLCQESLRYGISVQAEM
                     VHHIFPVSEYPELEFVEWNCLPLTNKKHNTFHDRVNDRVINQGLYWQKKRKKEFLNFF
                     KNEK"
     gene            17265..17657
                     /locus_tag="VQ846_000686"
     CDS             17265..17657
                     /locus_tag="VQ846_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HET3075315.1"
                     /translation="MAKPITAKSIKSKVVKQMKDLGTYRKEFEMIIDIFAGMLYQYQK
                     LAQDYADMGYPVTDTYVNKAGAENERKVPILTAMEILRKDILSYSNQLMMNPKSLGEV
                     VEQEGESVLTEVLKFKNEIKKKRVTGNG"
     gene            17650..19380
                     /locus_tag="VQ846_000687"
     CDS             17650..19380
                     /locus_tag="VQ846_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HET3075316.1"
                     /translation="MGNLDKAKKYAQHVLTHREEHCEENILAAERFFRDLENPTFEMD
                     EDMVDFVIHFIENVIVHQQGDDMFAVSIRNKPLLLQPWQHFVVVNLFGFYYKGTNERR
                     FKEALIMLARKNGKTSFTAAIALAYQILDTDSGSKCYIVANSVKQAMEAFGFLRFNVE
                     RWNDKNIRIKDNNQEHSITANFGDEGSFFIQALANDESRLDSLNGNVIILDEAHTMRN
                     SKKHGLMKKTMSAYRNSMLFVISTAGDIPTGFLANRLKYCQKVLKQLVTDDSFFIFIC
                     KANQSADGDVVNYLDENILKMANPSWGVTVSLKALKEEAEQAMNDPQTRNEFFNKTLN
                     IFTNSMNAYFNPDEFIASDSCYDWSLEELARLPIRWYGGADLSRLHDLTAAALYGVYH
                     DGEKDVDICITHAFFPRINAQKKANDDGIPLFGWQSDGWLTMSNTPTVLYDDIVKWFI
                     KMREKGFKIAAVGMDRKFGREFLTKMKQARFKMIDQPQLFYLKSEGFRRIEFKVKNKE
                     FYYLHSDAYEYCVSNVRAIEKVDDAVQYEKLDGDGGTARIDLFDASVFACIQALANLG
                     KGGDVMRFFD"
     gene            19388..19606
                     /locus_tag="VQ846_000688"
     CDS             19388..19606
                     /locus_tag="VQ846_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075317.1"
                     /translation="MNEIVLSEHEINLLINKGRVKVILNGEVVTIRQRHMKNLMAETV
                     KWEKQVIDVSQNIVRNKHFDSLFQNTFR"
     gene            19624..20826
                     /locus_tag="VQ846_000689"
     CDS             19624..20826
                     /locus_tag="VQ846_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HET3075318.1"
                     /translation="MGIFEKFWKRNKPSKSINMLSHSDLGLSNLMDSYVPLARNPDVV
                     TAVNKIADLVSNMTIHLMENTDKGDIRIRDGLARKIDINPCKHMTRKSWIFKIVRDLL
                     LYGDGNSVLHVEYEPVTDYISNLRPFPMREVSFQTDKDSYVISFRGEEYSPDEVVHFV
                     INPDPDIPYIGTGFRVTLTDVVQSLNMATKTKKSFMNGKNIPSLIVKVDSSSAELDSE
                     QGRERIAEKYLSTSRVGAPWIVPEALLDIQQVKPLSLTDIALNESVELDKRTVAGLLG
                     VPAFILGVGEFNKTEYNNFVNTTVMSIATTITQTLTRDLLLSSNRYFKLNPRSLFSYN
                     ITELSAVAQQMANSAAMRRNEWRDWLGMAPDPEMEELIVLENFLPQEKLGDQNKLKGG
                     EEENAKAK"
     gene            20810..21385
                     /locus_tag="VQ846_000690"
     CDS             20810..21385
                     /locus_tag="VQ846_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HET3075319.1"
                     /translation="MQKRNSYRATQFQTREEESGDLILSGYFIKFDEETELWPGYCEV
                     IKRAGVEKAVTDADIRALFNHDDSLVLGRTGNGTLTLGVDDVGLFGDIIINKDDPQAV
                     GAYARVKRGDVIGCSFGFIPVKIETEEREDGSYLDTVLELEIFEVSPCTFPAYPQTEI
                     AARQKDFESQKRANREALDKRKKEIKEKFNL"
     gene            21382..22554
                     /locus_tag="VQ846_000691"
     CDS             21382..22554
                     /locus_tag="VQ846_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HET3075320.1"
                     /translation="MNKLLILGARMRNKADKVVELEESIKELNKRSELEAKKLEQAGT
                     DEEVSAVEKNLEDIQKESDEKEAEKEQLEKEIEDLKNQVEELNRKAPTYPSQEKRGGQ
                     KLERRDAIAKYIRTGQTRDIVGLKTTDSGSAALIPTEVLKPHFVNKTRNPLLDLVERV
                     KVNSGSGKYPVIKKTDGVMVSIDELKSNPELGKPAISEIDYSIKTYRGYVPVSQEMID
                     DADYDIMSIVEDEVFNQGDNTELSLVTAVLKTATQADAAGFDGIKDIYNKKLKSIYKA
                     SIVVTKSMFAALDKVKDKDGRYMLQTDVASPTGYSFGGKTIYKVDDTVFGNEGDMKFF
                     IGDVTEFVKEFDRSQVSVKWVNNDIYGQLLGLFIRLDIKKADEEAGFFGTYTDVVA"
     gene            22566..22856
                     /locus_tag="VQ846_000692"
     CDS             22566..22856
                     /locus_tag="VQ846_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075321.1"
                     /translation="MSYKVIRPFKDLADPENHDYAVGDIFPREGYEPTDSFTNGLLTG
                     ANTAGSIFLEVLGDDEPKKPAPETKEVKEEPAVEQEETVEETAEESAEEVEE"
     gene            22859..23140
                     /locus_tag="VQ846_000693"
     CDS             22859..23140
                     /locus_tag="VQ846_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HET3075322.1"
                     /translation="MDQGQLLELLKLKLGISTDLRDKPLKKIISSVITELTDNLGIEL
                     VGERADHEMFIVDYAAYRYEGGVDMPRHLQWRLHNLQIASKKEVKNVES"
     gene            23127..23426
                     /locus_tag="VQ846_000694"
     CDS             23127..23426
                     /locus_tag="VQ846_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HET3075323.1"
                     /translation="MWNHEITLISKKVTGKDKLLQPISEDVEVTLLCRKKRVTRSEFY
                     QANQVGLKPSLVVEIRNFEYENQELAKFEGKQYHILKTYPIDSEILELTLSEVLK"
     gene            23423..23770
                     /locus_tag="VQ846_000695"
     CDS             23423..23770
                     /locus_tag="VQ846_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HET3075324.1"
                     /translation="MSNDLADLIAKELAAYSDEVTEEVDKIAEQVTDETVDELKETSP
                     KRYGKYRRSWKKKKLANGSFVVFNAVASLTHILENGHLSRNGGRVAGIVHIKPAEEKA
                     IQNFEKRIKEIGK"
     gene            23767..24090
                     /locus_tag="VQ846_000696"
     CDS             23767..24090
                     /locus_tag="VQ846_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075325.1"
                     /translation="MKLSDFAAILEQANLPVTYRAFKIGNAPDLPYLVYYESSPVINS
                     ADNTVNHQIKSVTVELAFESKDEDLEERLEELWANHKLFFEVQEETFIETERLYVKSY
                     TVYLY"
     gene            24102..24680
                     /locus_tag="VQ846_000697"
     CDS             24102..24680
                     /locus_tag="VQ846_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HET3075326.1"
                     /translation="MTQENKVTFGLENVHIAPIKTLAADGVITYGDVFRFPGAIELIL
                     DTKGETTPIKADNKDYHFMNSNEGYEGKLKIPHIIDEFATKILGEIKDPQTGVMTEKA
                     DASLTEFAMMFQFEGDKNKTRYVMYYCFASRPSLGSKTKNGTSTNERELSFKASPRPL
                     DTVVKRSITSADDKDAYDNWFKKVYEPTAVAA"
     gene            24692..25111
                     /locus_tag="VQ846_000698"
     CDS             24692..25111
                     /locus_tag="VQ846_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075327.1"
                     /translation="MRKIVLVGDQEYELGTNGYTPIAYKQQFGKDYFQDLFSMLKNQS
                     FMNELNKLETDKELTATNIDISMLSDFDMTFFNRLFWTFAKSANPHIKPYEQFFMEME
                     IFPIQEVGPVLMEMLNASMTTKKHQMSQNQLAKKSSQ"
     gene            25389..28148
                     /locus_tag="VQ846_000699"
     CDS             25389..28148
                     /locus_tag="VQ846_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HET3075328.1"
                     /translation="MAGNIKGIKIEIDGDTQPLQKALKNVNKAATDASQELRQIDKAL
                     KFDTGNVTLLTQKQEVLQKQVSTTKEKLETLRQAQSQVEQQFKNGDIGADQYRAFQRE
                     VEVTQNVLKGYEGKLANVNQALNENGSATQNNKNQLKELQNEQSQLASEMSKVTSSFK
                     LQESALGSNASEAERNALAQKKIGAQSEIVSKQISNLEQQLEITKKEFGENSTQANKM
                     ESELNQAKTAFNHLNDEMKGTKSAADSTQESLSEISRNLRAELLQQFSEKLSAISEKL
                     VEVGKEALEAAAQMQASNAQFTTVFGDMETQAREALNAIGQEMDIVPERLQGSFTQMA
                     SFAKTSGLDTAQALDLTSRVTRAAADGAAFYDKSIESVSESLQSFLKGNFANDAALGI
                     SATETTRNAAANKLYGKSFKDLSEAQKQLTLLQMVEDGNKLSGALGQAARESDGLENV
                     MGNLKQAGTNALSAIGQPLLEMMIPVFQTLATIVKGVAELFNSLPDPVKDFVVILGTV
                     VTAVGVIAPIFLTLQALAEFLKISIGEMIIAALPIIGTAIAIAAAVAAIIVIVKYLWE
                     TNEGFRDAVTTVWNAILAVINTVVSEISDFIMSIFGTVVTWWTENQELIRTSAETVWN
                     AIYTVISTILDILGPLLQAGWDNIQLIITTTWEIIKIVVETAINVVLGVIQAVMQIIT
                     GDWSGAWETIKGVFSTVWQAIQSIVQTIFSAIQSYISNILNGISGTVSNIWNSIKDTV
                     SNVLNAISSTVSSVWEGIKSTISSAINGARDAVSSAIEAIKGLFNFNISWPHIPLPHF
                     YVSGSANPLDWLSQGVPSIGIEWYAKGGIMTKPTIFGMNGNNIMVGGEAGNEAVLPLN
                     DKTLGAIGRGIAQTMGGTSPTINITITGNTVREEADISRIADEVAQRIADELQRRRQL
                     RGGFA"
     gene            28145..28867
                     /locus_tag="VQ846_000700"
     CDS             28145..28867
                     /locus_tag="VQ846_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HET3075329.1"
                     /translation="MIKHNELVIDGVRTSSFPFKIIVHDSPSIALGESKTALLEHGGV
                     SGAIVQTNKHRELVKKTYTIYLVKPTEEQMNQFMSLFIREKFWLESERVKTTRLWCYK
                     VNVSDLEEVQPGLYMTKATFTCHPTKYFKDSDTQRLTRSGTLTVQGSALAFPKITIVG
                     QSASETSFTIAGQVIRLERLTESLVMVNNPDNPSFKTTTGKPVKWSGDFITVDPAKVK
                     NIGVVLGPGIQSIEIETVWGWA"
     gene            28868..32089
                     /locus_tag="VQ846_000701"
     CDS             28868..32089
                     /locus_tag="VQ846_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HET3075330.1"
                     /translation="MLYLLNKDVRTVRWNGEPLHEATSAIVKETMNGDFTLTVKYPIS
                     DSGIYQLIQEDMLIKAPTPVLGAQLFRIKKPVEHNDHLEITAYHISDDVMQRSITQMS
                     VTSQSCGMALARMVQNTKTALGDFSFNSDIQDRRTFNTTETETLYSVLLDGKHSIAGT
                     WEGELVRDNFAMTVKKSRGENRGVVITTHKNLKDYQRTKNSQNVVTRIHARSTFKPEG
                     AEKETTIRVTVDSPLINSYPYINEKEYENNNAKSVEELQKWAQSKFSNEGIDKVSDAI
                     KIEAYELDGQVVHMGDTVNLKSWKHNVDAFKKAIAYEFDALKEEYISLTFDDKAGIGG
                     SRSSGGLSSAADAILGVTESAQEIALEKALQNADLDFDHKAGLLRQEISDDIELAKAR
                     AEEVKRELSDTINQRFNSFDNGPLKETKRKAEEALRNAGASTLLAQEAKRIGLDSVAR
                     LEAFKSQTTSAQTALSGDLDALKRTIANDIRPKQAQAEAEIAKQAEALSRTKNELAGA
                     STLLAQEAKRIELDSVARLEAFKSQTTSAQTALSGDLDVLKQTIANDIRPKQAQAEAE
                     IAKQVEALSRTKNELAGVKSAQATYKETTTRRLSELTNLANGKASKSELTQTAEELAS
                     RIASVQAGSSRNYFRNSRSRTFTTGGQAVYDYRTFIVPDFWKNSDRFKRDYVRISFDV
                     TFPVALVNDMPAMVHFSAHPWYAYRNLIFKGGTVERQHFEFTIDLSSSSEDYQTNNVF
                     IRFGTNYGFPAGLQVVIENAMLSVGNYFPAYQPAYEDQEDRVSVVESNFKQRADSLDA
                     GVRSLTEGLRTKADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQ
                     EILNATKDKASKSELTQTAEELASRIASVQASGRNLFLNSLFKQDIPKTGIWTTSTYT
                     ATIDSESKYLGHKALKIIGLNPSGRDGGNPKVTYPALGQFGKVIPGSTTNQDVTISFY
                     AKANKNGIMLRSRLGNIGYKTGNVTLSTEIKRYVVHIPKGWTNESKQTTNEWLFNFNQ
                     EGTIWIWMPKFEISDVDTSYSEAPEDIEGQISTVESTFKQRAN"
     gene            34085..35467
                     /locus_tag="VQ846_000702"
                     /pseudo
     CDS             34085..35467
                     /locus_tag="VQ846_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            35448..35651
                     /locus_tag="VQ846_000703"
     CDS             35448..35651
                     /locus_tag="VQ846_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075331.1"
                     /translation="MNTEQLNQALQMTIREMSTTSTDSMIASNFLSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            35654..36005
                     /locus_tag="VQ846_000704"
                     /pseudo
     CDS             35654..36005
                     /locus_tag="VQ846_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36015..36431
                     /locus_tag="VQ846_000705"
     CDS             36015..36431
                     /locus_tag="VQ846_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET3075332.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFVFLHSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            36435..36770
                     /locus_tag="VQ846_000706"
     CDS             36435..36770
                     /locus_tag="VQ846_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HET3075333.1"
                     /translation="MQQITEIITNGAISILVILAGVVVRVVKEYLVKKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKIRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGQE"
     gene            36770..37726
                     /locus_tag="VQ846_000707"
     CDS             36770..37726
                     /locus_tag="VQ846_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET3075334.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHYR
                     KDPELGFFSHVVGNGRIMQVGPVNNGSWDVGGGWNAESYAAVELIESHSTKEEFMADY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPDGLITVK"
     gene            complement(37996..38175)
                     /locus_tag="VQ846_000708"
     CDS             complement(37996..38175)
                     /locus_tag="VQ846_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075335.1"
                     /translation="MREVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(38317..38466)
                     /locus_tag="VQ846_000709"
     CDS             complement(38317..38466)
                     /locus_tag="VQ846_000709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075336.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            38748..39215
                     /locus_tag="VQ846_000710"
     CDS             38748..39215
                     /locus_tag="VQ846_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET3075337.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            39248..39345
                     /gene="ffs"
                     /locus_tag="VQ846_000711"
     ncRNA           39248..39345
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VQ846_000711"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            39402..39845
                     /locus_tag="VQ846_000712"
     CDS             39402..39845
                     /locus_tag="VQ846_000712"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET3075338.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            39847..40362
                     /locus_tag="VQ846_000713"
     CDS             39847..40362
                     /locus_tag="VQ846_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET3075339.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            40376..41737
                     /gene="radA"
                     /locus_tag="VQ846_000714"
     CDS             40376..41737
                     /gene="radA"
                     /locus_tag="VQ846_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET3075340.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFFIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            41810..42307
                     /locus_tag="VQ846_000715"
     CDS             41810..42307
                     /locus_tag="VQ846_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET3075341.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            42332..42526
                     /locus_tag="VQ846_000716"
     CDS             42332..42526
                     /locus_tag="VQ846_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075342.1"
                     /translation="MKKSSILLLCIGLQYETIYYTDGSRSGAEYGLMGISIFLALFYM
                     IPALYFLFRIGKNGNCQRRF"
     gene            42508..43131
                     /locus_tag="VQ846_000717"
     CDS             42508..43131
                     /locus_tag="VQ846_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET3075343.1"
                     /translation="MPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPDSTFLNA
                     FESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIGYIRTDL
                     PEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLAL
                     GTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            43276..44244
                     /locus_tag="VQ846_000718"
     CDS             43276..44244
                     /locus_tag="VQ846_000718"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET3075344.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
BASE COUNT        13835 a         7622 c        10162 g        12757 t
ORIGIN      
        1 atggataatg caagattcca tagaatgggg aagttagaac ttttatgcga agagtttggg
       61 cataaacttt tacctcttcc tccctactca cctgagtaca atcctattga gaaaacatgg
      121 gctcatatca aaaagcacct caaaaaggta ttaccaagtt gcaatacctt ttacgaggct
      181 tttttatcct gctcttgttt caattgacta tattagaggc gagacatttt tcggttcttt
      241 gtcaactgta gtgggttgaa gaaagcgaag atctagaaag gacaaatttc gtcctttctt
      301 ttttgaagtt ttcaaagttc ctaaaaccaa aggcattgtg cttgataagt ttgatgagat
      361 tattggtggc ttccagtttg gcgttggaat aaggtaattg aagggcgttg acgattttct
      421 ctttatcttt gaggaaggtt ttaaacagag tctgaaaaag aggtggaaaa gcaagagctg
      481 atagagatta tagtggtgtt tcaagtcttc ggaatagctc aaaagtttat ctagaatttc
      541 tttattagtc aagtgcatac gaaaagtagg gcgataaaat cgtttatcac tcagtttctg
      601 actatcttgt tgaatgagct ttcagtatcg cttgatagcc ttgtattcat gggatttcgg
      661 atgatggctt gtgttctgct ctcaagaaca gttatgatat tgagcttatc aaagtcttga
      721 gcaataaagc tcatttccat ctcccgattg aaacagtcac tccccggact gtttcaacgt
      781 cctaggacat aatctcagga agacgcgaaa aatcatgctc aaagtgaaaa tcattgttct
      841 tgcgaatgac agttgaagtt gaaatagaca actgatgatc aatgtcggtc atagaagtct
      901 ttttaattag cttctgagca atcttttggt tgatgataca aggaatttga tgattcttct
      961 tgacgataga agtttcagcg accatcattt ttgaacagtg atagcactta aaacggcctt
     1021 ttctaagaag aattctagtt tgaatttttt atactagaaa atcagaacca taatacctat
     1081 ataaaaatat tatagttcta ataggattta cccaaaagtt ttaaggcggt ctttttagaa
     1141 ctttaatcgt ttgaaattta ggtagcaaat ttgtttctat tttgtcaact tttcctattt
     1201 ttatcttgtt gaggctggta ttttaacaat tcaggaattg atagtgaatg tgtaaaattt
     1261 tttgttagaa taagtttata aaaaagaaaa ggagtatttg attatgttac aaaaaattta
     1321 tgagcagatg gctaatttct atgatagtat tgaagaagag tatggtccta catttggtga
     1381 taattttgac tgggaacatg ttcattttaa atttttaatt tattatttag tgagatatgg
     1441 cattggttgt cgtagggatt ttatcgttta ccattatcgt gttgcttatc gtttgtatct
     1501 tgaaaaattg gtaatgaatc ggggttttat ttcttgttga ggtaatttta gtaaatttcc
     1561 gaactaattt actcttttat ggaaagataa tagtaaatag ctagtaattt ttctaaatca
     1621 ttttttaata gttggaaata gcaaatcttt ctattgtttc ttcttgataa aaaggcgatt
     1681 ttttattata ataaattgta agatataatt gcaggtgaga gtcctgccat gtatgtgaga
     1741 aaggaagagc ctgagggctc agacaaaatt atgacttcag ttgttgttgt aggtacccaa
     1801 tggggtgatg aaggtaaagg gaagattaca gacttccttt cagcgaatgc agaagtgatt
     1861 gcacgttacc aaggtggtga taatgctggt cacacgattg tgattgacgg taagaaattt
     1921 aagttgcact tgattccatc tgggattttc ttccctgaaa aaatatctgt cattgggaat
     1981 ggtatggttg taaatcctaa atctcttgta aaagagttga gctatcttca tgaggaaggt
     2041 gtaacaactg ataacttacg tatttctgat cgtgcgcatg ttattttgcc ttatcatatc
     2101 gagttggatc gcttgcaaga agaagctaag ggcgacaata agattggtac gacaattaag
     2161 ggaattggtc cagcttatat ggacaaggct gctcgtgttg gaattcgtat tgcagatctt
     2221 ttagataaag atattttccg tgagcgttta gaacgtaacc ttgctgaaaa gaatcgtctt
     2281 tttgaaaaat tgtatgacag taaagcgatt gttttcgatg atatttttga agaatattac
     2341 gaatatggtc aacaaatcaa gaaatacgtg atagatacat ctgttatctt gaatgatgcg
     2401 cttgataatg gcaaacgtgt gctttttgaa ggtgcacaag gtgttatgct agatatcgac
     2461 caaggtactt atccatttgt tacgtcatca aaccctgtag ctggtggtgt gacaattggt
     2521 tctggtgtcg gtccaagcaa gattgacaag gttgtaggtg tatgtaaagc ttatacgagt
     2581 cgtgtaggag atggtccttt cccaactgag ttgtttgatg aagtgggaga acgtatccgt
     2641 gaagtgggtc atgaatatgg tacaacaact ggtcgtccac gtcgtgtagg ttggtttgac
     2701 tcagttgtga tgcgtcatag ccgtcgtgtt tctggtatta ctaacctttc tttgaactct
     2761 attgatgttt tgagcggttt ggatactgtg aaaatctgtg tggcctatga tcttgacggt
     2821 caacgtattg actactatcc agctagtctt gagcaattga aacgttgcaa gcctatctat
     2881 gaagagttgc caggttggtc agaagatatt accggagttc gcaatttgga agatcttcct
     2941 gagaatgcgc gtaactatgt tcgtcgtgtg agtgaattgg ttggcgttcg tatttctact
     3001 ttctcagtag gtcctggtcg tgaacaaaca aatattttag aaagtgtttg gtcctaagag
     3061 atttttaaga tttgtttaag ataggtcggg tatactatag acagttataa gaagacctcc
     3121 taacttgttg taacaaatat cctaaacttt tctttttcat aataatctcc cttaactcca
     3181 cccaatcagg tggagttttt tagctctatt tcaggctttt ggggactatt ctaaaaataa
     3241 tttttcgata tttttcggat tttggtcggg gaattggcgg ggactttttt agcgaatatg
     3301 actaagaaat aggtctgttg tcgcttcagc aagttcgtcc tctacttgat tgtaacgatc
     3361 cgtcatataa acctttgtat gccccagcgc ctggcttaat tgttcaagcg gaacccctgc
     3421 aataatgctt tgagtcgtga agaagtggcg catcatgtga ggtgttacat gcaatcctgt
     3481 tgcttcattc actagattga agtttctatt tagctgattt ggattgatga gaccaccttt
     3541 ctcgttgata gttatataat ccttgtgctg ttccttgata attcctaact ttcgcttaat
     3601 cttagaagct tcagctatca gataatagat caggtctgtt ccgatatcat caaggcagac
     3661 atatcgctct gaatccttcg ttttaagccc tcctttccct tttaaggtct ggttgcttcg
     3721 actgtctcta agatgcagta tagcccgtcc gctgtcgttc tgagtgatgt ccattggacg
     3781 caatccaaag acttctcctc ttctcaatcc aaaaattgtc agataggtca gagcgtagaa
     3841 ttgttttggc ataatctctt ctgcctttgc tatccaagtc ttgaactctt tgagagtcac
     3901 tttcttgttt gcagcaggga tatcactctg gccgatgaaa acacctttca agcgatttga
     3961 gagcagattt ccatttttga cggcatcatt cagcaatgcc atgaagctgg aattgagggt
     4021 ttgaacagtg tatctggtat ggttctgcaa cttttcagcg ataaatagtt catactcatt
     4081 tctatccaaa tttttaagca ggatagaacc aaactttgcc ttgatatggt tcttatagag
     4141 attgtcattg aggtagtagg aagtgtcatt ccagcgccct gttgacaatc tcttttcaga
     4201 atagatatcc caatattgat caagcgttag attcgtattg atacctaatt cttgttcttg
     4261 gatttgttgc tcaagctcta ccaaggctgc acgagcttga gggagagttg tgaaaccact
     4321 tttacttttt tctctttttt tacctcggaa gaaaaaagaa cgtctgacat agtaacgctt
     4381 gcctttagca gtctcatagt aatagatatt tgggtatttt gttttattat atttcattgt
     4441 attctccttg tttatcggct tctggacaag gtctaaacat tgagaatatt gacatcaccc
     4501 ctttcatggt gtaaaatagg gtatagaaaa gaggcctttt taatggctga ttttttataa
     4561 gggtaagctt cacaatcaaa ctttggcgag ggcgattgtg gggctttttt tttattttct
     4621 gaatagtttg tatagtttat acaaactcca agctgtcaaa gggactgcaa ggaatggagc
     4681 gattggaata ccaacaactc caaaaaagac taagaaaaga tacagtgctg aaaatatgat
     4741 tttattggca ggaagttttt tcttaggaga tgtttctgaa agattaatct cgtttactat
     4801 atcagcgaca ctgtcactaa caatattttg ttcaatatgt tcagtttcag ttgttttagt
     4861 ttcaatagct tcagtttccg taacttcttt attggaaatg ttatcatcaa ttatctcaga
     4921 tgattcgctc tgaattactt ctttttgtga agaatcaaca atggtaaggt aaatttcaaa
     4981 accaacatcc aaatcattat ttgtgacaac tttttcttca tactcgtccc attctttata
     5041 tgggccacct tttatctctc cctctattac aaagttatat tttttattat caaaatattt
     5101 tcgtattgtt ttagcaatgg ttgcaggaac atatcctaca tgattatcaa atatcaaaac
     5161 ttttatagca ttaggatcat atctattatc tacttctgga acgagctcaa tattgaaaaa
     5221 gtctaaatct tgatatttga aaactttcag accatattct tcaagttctt ctctaagttc
     5281 cttagttgat aaattgctat agtactttgc atattctggg attccgttat cttcagcaat
     5341 catattgcaa gcttctttta ctgctttttt atattttgtc actccagcga ctctgaataa
     5401 tattttttca acagtctttt gcatatattt cccctctata taaatatctt caatgcaatt
     5461 tttaatttac taatgccaag tactcttcct tgaccatgac ttcattagtc atagttttta
     5521 gattgtagta agacatgaat ttgaggtaat caaactctgt ggggtcgtct aagctttcta
     5581 gtgcgtcttt cacgagatga tggatcatat tcctatcagc ttcgttttca cagcgtaggc
     5641 gagcgttttg gtactctgag cgtgtatggt ctttgtgtcc gagttagtgt agggcgactt
     5701 ggatttgttg ttcaggagct aagttgtggt caatagcgag tacgtttgtg tctggattgt
     5761 agaaaccgct actgtgccag tttgaaccgt cgaagagaca aagctctact tgatattctt
     5821 cgcagagttt agcgagtatc atagttctcc ttcgttattt ataaacatct ctgcggtggg
     5881 cgatttccac ggctaggacg actagtttat cgtcttggat atcacagatg atgcggtaat
     5941 tctctactct gtatcgccag taacctgcaa ggttggcttt tagtgctttt ccgtgttgtc
     6001 gtggattggt cgtgttttca atgtttttag caagccagga taggatttgt tttctggttg
     6061 gggtatctag ttttttaagt tgcttgagag cttttttatc aatatctagc cgatacatta
     6121 agcaatatcc tctcgagata gtcctagttc atccaagacc tcatccatgg tataagtagc
     6181 tgggtcggct aaatactcca tataggcttg gtcggctgct cgtgcgtctt cgatatcttc
     6241 catgagtacc atgaagtcgt caaaatccat ggttgttgtg tcgataccgt gtttggttag
     6301 gtagtccgtg atgtaggagt ttttttctgt gaaattgatc gtgatagtca ttagcgttct
     6361 cctttgcttt tgaagtgggc ggatagaacg gatgtgatga agtcgatatc atcttcattt
     6421 agtggttttc cgtcaaataa catggtgttg gctgctgctt tgcgtaagtc tatgatttgt
     6481 ccatttactt gagcaaattc atcacttcca gcgatagcag gattatctgt ccgtccgagt
     6541 aggtagtcag cacttacatt aaaataatca gctatttctt ttaaaacttt tgaactagga
     6601 ttacttcttt ttaatcgata taaagtatta gttccataac caagtttttc ttctaaaata
     6661 tttatagaaa tcccctgttt atcagctaat tcttttattt tatcgaatgc tacaaacatt
     6721 aatttttcaa cctttctaag caaacgaaaa ataattttaa aatatttgta gaaaatagtt
     6781 gacttattta gtcaaatgtt ttaaaataat attcgtaagc taaagagtta gcgaactaga
     6841 caactaaaaa ataaagcctt acaaaactga ttggcgtccg tttactgtag gtaaaactta
     6901 cttttagtag gtcttttctc tatgttttta ttttaaaaca tttgactaga gttgtcaaga
     6961 agttcgctaa ctttttagat aattttttaa aaggaggtca gggatgaacg aagaagacct
     7021 gaaagaatta ttggaactct tagcgacaga ttatgggcga gggtatctag atggagtagt
     7081 tgggggactt tcaatgcttt tgaaaatttc aaaagaagca gagaaatata gaaaggaaaa
     7141 atatgagtaa agcaaaatta ttagtcacat aaagtaggta aacgaggtag gaaatgatag
     7201 aaacactgga aataaaaata aacaaaaaga catacttcat atctgcaagg gcagatgttc
     7261 cagtcaggta taaagttatg tttggcagaa atctcttcga tgacttttcg aaaataaaaa
     7321 acaataaaga tgaaactcta tcactttgtc tattattttg tatgacagac aaatcgtcag
     7381 aagaattctg taaatttttt attgaaaata attcatatat aacaagtaga catcactatg
     7441 aggcaataag attatttgaa aaacatttta tctttaagcc cgtaacatat tcactagagg
     7501 acatagttca aatcctaaca atatatctag aaaatactaa aacacagatt ggaggaaaaa
     7561 atgagtaaag aactaaaaat aattaaggct aaaattaaaa ctcgtttgat tgagttggat
     7621 atgactcaag tcgaattggc aaaacaagta tctgtagcat catcagttat ttcagagttg
     7681 ctgaagtatg gaaaaggaag tgattatgtg aaagaaaaag tcgtagatat tttgggtatt
     7741 gaaaaccctt gggaaaatag ttagagcatg aggaaatagt atgaacaacg cagcgcaaaa
     7801 agtaacacgg attgacaaag atgcctggga gattgctacg gagctggcga acgagtacgg
     7861 cgtatctatt tgtcacatca tcagcgagag cgtccgctac tgtgcagaga atgccgaatt
     7921 taaggagatg gacgttgtcg ttaaacgatt ggtagtcggc agtaaggtgc tggagtagga
     7981 gggtggagat gaatgaacta gtatggtttt actttgttgt cataatcaat cttatcattg
     8041 gttttgctac atactacgct agcaaaagag atagagaaaa gcgcatcaac gggtttaaaa
     8101 aaatacaaga tgatgagctt gaaagagtta gaaataaatt taatttatga ttttttagaa
     8161 gtcttttgaa taaatctttt atttaagttt tgtttgctat tgcttgcttt attagctatt
     8221 tttaaagctt tgtcaaaatc aacctcgcct gtaaaaactt tgtaggtaag atcattcatc
     8281 ttcatagact tgtcggtttc agtatcaagc tgagatacct tctcaagttc ttgcaaccgt
     8341 aattcatgag cttgtttgat tttttcaagt tcaaaggcat gttgctgttt tagagtatct
     8401 atctgatgat gaaattctct ttcgagtttt tcaacagtat gtgaatgttc tttggcttgt
     8461 ttttcaatct cagctttatt gttagctttt gatgcgacat atgaccataa ccctgatatt
     8521 attgcaagaa taatactaat cacaggttga ataagagctt gatattccat aagatttctc
     8581 caatcgtttt attttaatta taccaaattt agaaaggaac tttatgaacg aaatttttaa
     8641 ttttcacggg caggaagtcc gtactttgat cattgatgac gaaccttggt tcgttgggaa
     8701 agatgttgca gacatcctag gatatagcaa ggctagaaat gcgattgctc ttcatgttga
     8761 tgaagatgac gccctaaaac agggcctcac agataattta ggaagggttc aagaaactat
     8821 catcatcaac gaatctggcc tctactctct tatcttatcc agcaagttgc ctcaggctaa
     8881 agagtttaag cgctgggtga catcagaggt tttgccagct attcgcaagc agggcggttt
     8941 cattcgcgag gacttggacg aggatgcctt tatcgctctg tttactggcc agaagaaatt
     9001 acgtgagcaa caggcgacca tgctggaaga tattgactat ctcaagagtg agcaaccgat
     9061 tcacccaagc tatgctaaat cgctcctgaa gaagcgtaag gctcgggtcg tggtttgctt
     9121 aggtggcatt gatagtccgg cttatgcgga taagactttc gctcagtcag tctttagaca
     9181 agctgagatt gactttaaag accacttcaa cattaatcgc tatgacttgc taccgaaaaa
     9241 attcgcagat gcagccttgg cctattggat gacttgggag ccaagcacca ataccaagat
     9301 gaaaatcatg aaattgaact catttgacga agggtaggag gggaagaaga tggacaatgt
     9361 tctactttca ctgtctgaat ggattaaatc cattatcaag gacacaatca caaggctagt
     9421 cgaaatagaa aaagatagtg accactatcc agagctgatg gatgtgaaca ctacctgtga
     9481 ttttctagga attaagtatg ccaccttttc agataattat cgttacttaa agggatttcc
     9541 aaaggaatta cctggtaaga aatggtcaaa aagagccatc aaagaatggc tctctaatca
     9601 aatataataa ctttactaaa aggcttctgg acaaggtctt agcaaaatta tttgactata
     9661 ttatagcaca aaaagaggat aaggagataa aaatgtttga accaccgatt ttagaccagc
     9721 tgatgggggt tggagcctta ctgcttggat ttgcaggggc ttgccgtcat atcaaattgc
     9781 aggaacaacg caaggaagaa gaaagacgag aagagcaaga atttgcgtct atgattatcc
     9841 aagtgcgtaa tcatgcatat gaacgtggta gagaggacaa atggcaagaa attcgcaaga
     9901 atattcgcag agagttcaaa ggattcacat atgacaacga accgcctgta ggcttgcgtc
     9961 ctgagccgtt agctttgcca gaacctaaac agtctgcaat cagatttttg taatgaggag
    10021 gtcaggaaat gcaagaattg attgaatcgc tggataacct gattatgatt gttaaagaac
    10081 tggaaggaag ggaatcaact tcaagacatt ttattacgat atgggaaaac gattataaaa
    10141 atctattacg agtcaaagaa tacctaaccg actatgaaaa actagcaaag gactatcgtt
    10201 atatgaccct taaaaataag ctgctaaaga ttgaaaaaat ggagctggaa ggcaggtaca
    10261 tctatgaaga tatgcggatg aagtatcgtg ccaatcgcag gaagtggggt gctcggtatg
    10321 tctgaaatca agtggataaa aatcaaaacg gatatttttg acgatgaaaa gatgtgtcta
    10381 attgatgcct cgcctgatcg tgatgcaatt attgtaatct ggatcaaact tataacacta
    10441 gcaggcaaat tgaatacaaa aggcgtacta accatttcta aaaacattgt atacaccgat
    10501 gaaatgcttg cacaaacgtt ccaccgtccg ttgaatacag ttcgtatggc tcttgaagtt
    10561 tttgaaaagt ttggaatggt tgaaaaaatc gatggagtga taatgttacc caattgggag
    10621 aaacatcaga acattgacgg catggaaaaa ataaaagagc aaaatcgaaa tagagcagca
    10681 cgtcaccgac aaaaacagaa attacttgca cagaacaacg aaagtaacgt tactgataac
    10741 gttatggata acgttacagt aacgcatggt aacgcactag ataaagaatt agataaagat
    10801 atagagataa ataataataa ggtgatgatt agttccagcc tttctgaaaa tttgaaaaat
    10861 agcggaatcc atctaactga taagtcacat caacagttac ttgattatgt gggacttgat
    10921 ggaatgagtt tcgatatgtt gaaccgtgca gtcgagaaaa cttccggatc acacaaacct
    10981 agtttcaatt acctaatagc cattcttgaa agttggaaaa agaaaggctt cacatcgatt
    11041 gagcaggtgg atgaggacga ccgtaaatat aaagagggta agaagtcaag acaacaaaac
    11101 gataaaacat cggaacagga ggtaagggac gaatggggat tttagaacta attgagcagt
    11161 tcgagattga ctattatccg ttaagctacg agaagaaaac tcttttagca gaccaaccaa
    11221 ttcatccagt ggttgcctgc ttgtctgaaa tggctagctg gcatgaatgc ggaggtcggc
    11281 tagtatggta gacaatgtgt ttgaggaaat cgccttatct tatcgtagga atacagaaca
    11341 acaagaagag ttctgcgaaa agcataacat ccctttgata aagatattga ggaccgagag
    11401 tgttgtatgt cgcatgtgtg aatctgagcg gattcatgaa gaaaatcagg aaagagtgaa
    11461 tgaactggct aatgctgaga atgagcgaga gaggaaatac tatcttgaaa agttctctct
    11521 ttatgatgaa attttgaaaa atgctacttt ggacaatttt gaaacaccaa ccgaaaaaga
    11581 agcggaaaag ctagcttttg caaagaggat ttgtcgtgag tggtctgagg gtgctaggaa
    11641 caacattgta cttcagggag aagctggaac tggtaagagt catttggctt ttgctatggt
    11701 taaggctcta tctgagtaca cgaaagagat tgctattttt attaacgtaa ctgacttgct
    11761 gatgaagatt aaagctgatt ttagtcagga agagtttctg gtcaataaaa ttgctagtgc
    11821 taagttcttg gttttggatg atttaggaat ggaaaaagat agcgaatggt catttactat
    11881 tctctacaat atcttgaata agcgttcaaa cacaatcatt accacaaatt tgatttctgc
    11941 cgatattcaa aaaagatatg gcagaccttt catgtccaga ctaatgaagg gtgtagataa
    12001 agaccatttg atggttttca atgatttgac aaacaagcga aagcaatatt tttagaatag
    12061 gggtggctga tgtttatttt aaagcatggg acaagagagg ataagccgtt tctaaggtct
    12121 gcaattatca gtgtgactgg cttggatatt tcgtgttctg aggagaagaa agccatgcgg
    12181 tttatttctc gggcggcagc cttacaggtt agcaaggcat tgagaggttc ctttgggaac
    12241 ttctaccctg tcgaggtgga gtgatgttag agctttattt cgtctacaac gggcactgta
    12301 agtttttcct tgggaggttt gacaatgtcg atgatctcat tgagcagatg gaagaccatc
    12361 agtgggcttt ctcagctatc actcatccaa ggtttcagaa gcacattggt cagcggacga
    12421 cacgatttga ctacggttct aaggattgtt actatttagc gactttttca ggaggagaaa
    12481 aaaatgattg aacttattaa agaatttgga atggctattc tgtggttatt tcttggctat
    12541 ttagtcgggg aacgtgcagc aagaaaggaa aagaaagatg atcaataacg ttacattttt
    12601 agtgggaggt aagaaacatg gttggagtaa cctatcagga aattcatctc tttgttgaat
    12661 ttttgaaaga gcagtacggg caaggtcgtc cagactatat tgaagccctg aacgacttag
    12721 acggtctggt ggaagtctcc tacagagaag ctattgaaag atttttagaa gatgaagtac
    12781 gataaacagg ctgagattga cggactgaaa cgcacgatcg agcaaaacga agagaagata
    12841 atcgagtatt cgaagccgtg cgatgcacgc aagagacgga ttagagcgct ggagcgcgat
    12901 ttgttgagga aaaagaataa agaattaaga cggaaagcgg aggagttgga atatgatgga
    12961 agagttaaag caaaaagtta atgaagtata caactggacg gtagaagacg ggaagccgca
    13021 acctcccaag caagatttac cacaagcggt gaaagaccgg gcggactatt tttgggaaat
    13081 ggcagaagat ggtatgacgt ttatgggagc gatggaatgc atcttcgctg atgaaaagcc
    13141 tacagactat gatttgggag ctactaaggg ttggttgcca aaatctaagg agtttgatga
    13201 ttgggttggc tattcgccaa gcatgtctca gttagttatt gcagtttatt tgatttacag
    13261 aggaagcgaa gatgaaactt aatgaattga ttaagaaata taaaaaactt gagggtgtat
    13321 ggaatgctga aggagcagaa ctagttcgtc aaatttttct gcaagacttg gaacaactag
    13381 ataaaccgca accagtcaaa gttccgcagt gtgtggcgga atatatagaa tttaaaaaga
    13441 aaaacaattt tcatgtttac ggtcttgctt ggcttaacgg ctacgaggtc gaaaaagaga
    13501 agcggtattt tgttaagatt aaagggaata ttaaagaaaa tatgttggtt tatggagaac
    13561 ttttgaaaag gtatttcttt acaaaaagct ttagtttaga cgatgttata tattcccaca
    13621 ctcgtaaaga actagaagcc gcaaacttcg gctgggtgtt tgattgccca ggtattgaga
    13681 ttgaggaggt ggaataatgg gagatatacg aatactagac gcttgttgtg gcagtcgtat
    13741 gttttggttt gataaaaatg agagtcatac aactttcatg gacatcagac aagaaaaatt
    13801 tgaaatacat ggaaaaaggt aaacgtaaac cctgacgttg tcggagattt tcgtgatatg
    13861 ccattcgatg gtgaaacatt taatctagta gtatttgatc cgccacattt gaaatgggca
    13921 ggtcaaaact caattatgcg atcacaatat ggccagctgg ataaagttac ctggtcggaa
    13981 gatttagcca agggttttga agaatgtatg agagttctaa aagttggagg tactctagtt
    14041 tttaaatggt ctgattgtca aatcaacgtt aaagaagttc ttaaatctgt accttttaaa
    14101 ccattgtttg gacaacaaag aggtactacg cattggatga cgtttatgaa atttgagaag
    14161 gtcacagaga gaaaggacgg cacgtttgta aaagaggagg atttggcatg ataccgaaat
    14221 ttagagtgtg gcatcatgaa ttaggtagac tgatgtcagt caaatgtatg ttttttcagg
    14281 atagcgagat tgaagaattt gagttaaacg atgctttaat gaatgattac attacagctt
    14341 atcctgatga aatcgaactc atgcaatcaa caggactcaa agacaagaac ggcaaggagg
    14401 tattcatcgg tgacatcgtt aaatgtacaa gaggatgtct ccatgaagta tatttagaaa
    14461 aagaatacgg tggtacattc ataggtggaa tgcctgctgt atacctaaaa ggacttggag
    14521 atggatatgc gtggacggaa tatgaggaaa tcatcggcaa catctacgaa aatccagagc
    14581 ttttggagga taaataatga acccagaaat aattgacaat ataaacaaac caagccacta
    14641 ccaaggtgca aacggtcttg aggctatcga tgttgtgcat aacttcgttg ggagcctttc
    14701 cggagcgtct gctttctttt ggggcaacgc aatcaagtat atgttacggt ttcaaaagaa
    14761 aaatggtctg gaagacctga agaaagctag aaagaatctt gactggctga ttgaggagat
    14821 ggagcatgag tgaatacgca ttgtatgaag gcgacacctt tattaccatg gggacgcttg
    14881 cggaaatcag caaagaaaca ggaattgctg aacggtgcaa gtatttcaga gtttgccatc
    14941 gaagatatct gtacgttttt tgggattgag atccaagaaa ggaaataaaa gtcaatgaac
    15001 aaaagaatta agaagaagat agctaaacgg cagatacaag aaaagcaaga agaattatac
    15061 aaacaactac ggaaattaag ttctgaagaa attgaagcta taactaaaat gattaatcag
    15121 gcagtttcta atattcgcaa ggctttctct cagatatttg ataacttgtt tacatttttg
    15181 aaaaacttgg aggtggaaat tgaaaaaatt gagcgacgaa gaactcaaaa cgttagacag
    15241 agaacttttc aaatttcaaa acattcaacg cacaatagac ttaagaaggc tagaattaga
    15301 aaccagaaac ccagatgctc aaagtgggcc aactgtagga ataagcaaac ctaccgaaac
    15361 tatcgcaatc agaatcgcag atgatccgac tttaaaattt cttgaaggat ttaaagggat
    15421 tattaacaaa ctcttaagca atctagtcga ggaggacaaa gaaattttca atctacgctg
    15481 gagataccct caactgagat gggaagaaat agcagaacag aaattcatga gcaaagctac
    15541 aatctatcga cgtagaagga ttatcttaga acagtacgct ataatgaaag gtgagttgta
    15601 aataagattg agacaaaaga catcttgaag tctcacaaaa aaagggctat tatgatagca
    15661 tgaacttctg aaaacaaaaa cacaaatcac atgttggagt tatcctattt ttacagaaaa
    15721 gttgtttaac agaggaacat catgagtcag caaccagctg gctttttgtt ttgtagaaag
    15781 gaggcagtta tggaatttgt atcaccgata aaagacaatg ataatattca agctatgaaa
    15841 gattatctca gagaatggaa tgagatgtat tatatgctat tcatcacagg cctgaatact
    15901 ggcttacgag tcggagatat acttaccttg aaagttaaag atgttcaagg ttggcacatc
    15961 aaactgagag aacggaagac tggcaagcag ataacaagac ggatgacaaa agaactcaag
    16021 aaagaaatga ggagatatgt tgaaggcaaa ccatttcatc atttcttatt caagagtagg
    16081 caaggtcaga ataaagcgat cactcgtgag cgagcctatc aaatcataca tgaagcagct
    16141 gaagaacttg gcattgataa tgttggcaca catacaatgc gcaagacatt cggctataaa
    16201 tattacaaca agacaaagga cgtaggaaca ttacagaaaa tgttcaatca ctcatcacct
    16261 gcaatcaccc tgagatacat agggatagag caagcagagc ttgatgacgc actacggaac
    16321 tttgtcattt aattttttag atattacttt cacataatga gttaagcata aactgaaaaa
    16381 atgaaagtct tcaaaaccta tgattagtaa gggtttgaga tttagagtga gtttaacaaa
    16441 atataagata tgtgaaagtg agggataaaa ttggtatagt tacaagaggt gaaaaatggt
    16501 aaaagaatac cgtgatgatt ttcttggaga aaaggccttc gagaaattaa ataaagatat
    16561 tgatgccaat cctgaagttg gctttgaaat tgttggatat actcaaacag catttgtaaa
    16621 tggaatgcat ataccgttaa cagccatact agtaaaatgg aataattttt ttaaagaatc
    16681 agaatgagac aaaagacatc ttgaagtctc acaaaaaaag gtttattatg gtagcataga
    16741 tttcttgtat gaggagggga taggtcaaag gcctatccct tttagcattg agaaaggagg
    16801 tttgagatgt ataacaaacc tatcagacca tccttgaaat ctaagaagtg ggagaagttc
    16861 cgtgatagga taatgcgtaa gcatgattat ctttgccaag aaagtttgcg ttacggaatt
    16921 tctgttcaag cagaaatggt acatcatatc tttcctgtgt ctgaatatcc tgagcttgaa
    16981 tttgttgaat ggaattgctt gccattgacg aataagaaac ataatacgtt tcatgataga
    17041 gtgaacgata gagtaatcaa ccaaggattg tattggcaga aaaaaagaaa aaaagaattt
    17101 ttaaattttt tcaaaaatga aaaatgaaaa tttttaatcc ccccctcttt ttgaaaaatc
    17161 attttggcca gtagggtact ggtgaaggga actttttcca agtcgggggc cttcaaacaa
    17221 aaagggggta aaaactaagc gattttgacg aaaggaggta gtttttggct aaaccaatta
    17281 cagcaaagtc gattaagtca aaagtggtca agcagatgaa agacttggga acttatcgta
    17341 aagagttcga aatgatcatt gacatttttg caggtatgtt ataccagtat cagaaacttg
    17401 ctcaagatta tgctgatatg ggttatccag taacagacac ctacgtcaat aaggctggtg
    17461 ctgaaaatga gcgcaaagtt ccaatcttga cagcgatgga aattttgagg aaagatatac
    17521 tcagctactc taatcagttg atgatgaatc ctaagtcgct cggtgaggta gtagaacaag
    17581 agggtgagtc agttcttact gaggtactga agttcaagaa cgaaatcaag aagaagcgag
    17641 tgactggcaa tgggtaatct tgataaagct aaaaaatacg ctcaacacgt cttaactcac
    17701 cgagaagaac attgcgaaga aaacatcctt gctgctgaac gttttttccg tgatttagaa
    17761 aatccaacct ttgagatgga tgaggatatg gtggattttg ttattcactt tatcgagaac
    17821 gtgatagttc atcagcaggg cgatgatatg tttgcggtat ctatccgtaa caagccattg
    17881 cttttgcaac cgtggcaaca tttcgttgta gttaatctgt ttggttttta ctacaagggt
    17941 acgaatgagc gcaggttcaa agaagcgctt atcatgcttg ctcggaagaa tgggaagacc
    18001 tcgtttactg ctgcaatcgc acttgcttat cagatattag atacagatag cggttccaaa
    18061 tgctatatcg ttgctaactc agtcaagcaa gcgatggaag cttttggttt tttaagattc
    18121 aacgttgaac gctggaacga taagaacatt cgtatcaagg ataataacca agaacactct
    18181 atcaccgcca attttggtga tgaaggttct ttctttatcc aggctttagc gaatgatgag
    18241 agccgtctgg actctttgaa cggaaatgtt atcatcttgg acgaagctca cacaatgagg
    18301 aacagtaaga aacatggtct tatgaaaaaa acaatgtcag catacagaaa cagtatgctt
    18361 tttgttatct caacggctgg ggatattcct accgggttcc ttgctaaccg tctgaaatac
    18421 tgtcaaaagg tgctcaagca attagtcact gatgattcat ttttcatctt catctgcaag
    18481 gctaatcaat ctgctgatgg ggacgtggtg aactatctgg acgagaatat cctcaagatg
    18541 gctaatccgt catggggtgt cacggtttcg ctcaaggctc tcaaggaaga agcagagcag
    18601 gctatgaatg atcctcagac aagaaatgag tttttcaata agaccttgaa tatcttcact
    18661 aactctatga acgcttattt taatcctgat gagtttattg cgtcggatag ttgctacgat
    18721 tggagtttag aagagctggc acgtttgcct attcgttggt atggtggtgc ggacttgtca
    18781 agattgcacg acttaacagc tgctgctctc tatggtgtct atcatgacgg tgagaaagac
    18841 gttgatatct gtatcacaca tgctttcttc cctcggatta atgctcagaa aaaagctaat
    18901 gatgacggga ttccactctt tgggtggcag tctgacggtt ggttgacgat gagcaacact
    18961 ccgaccgtcc tctatgatga tatcgtcaaa tggttcatca agatgaggga gaaagggttc
    19021 aagattgctg ctgtcggaat ggataggaag tttggccgtg agttcttgac gaagatgaaa
    19081 caagctcggt tcaagatgat tgaccaacct cagctttttt atctgaaatc agagggattc
    19141 agacggattg agtttaaagt taagaataaa gaattttact atcttcattc tgatgcttac
    19201 gaatactgtg tgagcaatgt tagagcaatt gaaaaggtgg acgatgctgt gcaatatgag
    19261 aaattagatg gagacggtgg gactgcaagg attgacttgt ttgatgccag cgtctttgct
    19321 tgtatacagg ctcttgctaa tcttggcaag ggtggtgatg tgatgagatt ctttgattag
    19381 gtgaattatg aatgaaatag tattatcaga acatgaaatt aacttgctga tcaacaaagg
    19441 gcgagttaaa gtaattttaa acggggaagt agtaactatt cgtcaaagac atatgaaaaa
    19501 tttgatggct gaaacagtaa aatgggaaaa acaggtaatt gatgtcagtc agaatatcgt
    19561 aagaaataaa cactttgatt cactttttca aaatactttt cgttagaaag gaggtgagga
    19621 aacatgggta tttttgaaaa gttttggaaa cgaaacaagc caagtaagtc aatcaacatg
    19681 ctgagtcatt cagatttagg gttgtcaaac ctgatggact cgtatgtacc tttggccaga
    19741 aatccagatg tggtgacagc ggttaataag attgctgatt tggtctctaa tatgaccatc
    19801 cacttgatgg agaatacaga taaaggtgat atcagaatcc gtgatgggct tgctagaaag
    19861 attgatatca atccgtgtaa acacatgaca aggaagtcat ggattttcaa gattgtgcgc
    19921 gatttgcttt tatatggcga tgggaactct gtcctacatg tggaatatga acctgttacg
    19981 gattatattt ctaatctaag accatttccg atgagagagg tttcattcca aacagataag
    20041 gattcctatg taatctcatt taggggtgaa gaatattccc ctgatgaagt agtccacttc
    20101 gtcatcaatc cagatccaga tattccatac attggtactg gttttagggt gacgttgaca
    20161 gatgtggttc aaagtttgaa catggctacc aagactaaaa aaagctttat gaacgggaag
    20221 aatattccta gtcttatcgt taaagtagac tcgtctagtg ctgaactaga ttcggagcaa
    20281 gggcgtgagc gtatcgctga gaagtatttg agtactagca gggttggcgc tccatggatt
    20341 gttccagagg cattgctgga cattcagcag gtaaaaccgc ttagtctaac ggacatcgct
    20401 ttaaacgagt ctgtggaatt ggataaaaga acagttgcag gtctattagg agtacctgct
    20461 tttattttgg gtgtaggaga gttcaacaag acagagtata acaactttgt aaatacgact
    20521 gtcatgagta ttgctaccac tattactcaa acactaacca gggacttact tttgtctagc
    20581 aatcgttact tcaagctaaa tcctcgttca ctcttctctt acaacattac ggagttgtct
    20641 gctgttgctc aacaaatggc aaacagtgct gcaatgcgtc gtaatgagtg gagggattgg
    20701 ctagggatgg ctcctgatcc tgagatggaa gagttgattg ttcttgaaaa ctttctccct
    20761 caggagaaac taggagatca aaataaactg aagggaggtg aggaagagaa tgcaaaagcg
    20821 aaatagctat cgtgccactc aatttcaaac gagagaagaa gaatctggtg atttgatttt
    20881 gagtggctac tttatcaagt ttgacgagga gacggaattg tggccaggct actgtgaagt
    20941 tatcaagcgt gctggtgttg agaaagctgt cacagacgct gatatcagag ctttatttaa
    21001 ccatgatgat agccttgttc tcggtcgaac aggtaacgga actctgacac tgggtgttga
    21061 tgatgttggt ctttttggag atatcatcat taacaaggat gatcctcaag ctgttggggc
    21121 ctatgcccgt gtcaagcgtg gagatgttat agggtgtagc tttggcttta tcccggtaaa
    21181 aatcgaaacg gaagaacgtg aagatggttc atatttggac actgtcttag agctagaaat
    21241 ctttgaagtg agtccatgta ctttcccagc ctatccacaa acggaaattg ctgcacgaca
    21301 aaaagacttc gaaagtcaga agcgtgctaa tcgtgaagcg ctagacaagc gcaagaaaga
    21361 aattaaggag aaatttaatc tatgaacaaa ttattgattt tgggcgctcg tatgcgcaac
    21421 aaagcagaca aagttgtaga gcttgaagaa tcaatcaaag aattgaacaa gcgttctgaa
    21481 cttgaagcta agaaattgga acaagctgga actgacgaag aagtttcagc agttgaaaag
    21541 aaccttgaag acatccaaaa agaatcggat gaaaaggaag cagaaaaaga acaacttgaa
    21601 aaagaaatcg aagatttgaa aaatcaagtt gaagaactaa atcgtaaagc accgacttac
    21661 ccaagtcaag aaaaacgtgg aggacagaaa ttggaacgac gtgacgcaat cgctaaatac
    21721 attcgtactg gtcaaactcg tgacattgta ggcttgaaaa ctactgattc aggaagcgca
    21781 gctttaatcc ctactgaagt tttgaaacct cattttgtta ataaaacacg taatccactt
    21841 ttggatcttg tggaacgtgt gaaagttaat agtggatctg gtaaatatcc agttatcaag
    21901 aaaacggatg gtgtaatggt ttcaatagat gaattgaaat caaatccaga actcggaaaa
    21961 ccagcaatca gcgagattga ttattcaatc aagacttacc gtggatatgt ccctgtgtca
    22021 caagaaatga ttgacgacgc agactatgac atcatgtcca ttgttgaaga cgaagtgttc
    22081 aatcaaggtg acaacactga attgtcatta gttacagctg tcctcaaaac agctacccaa
    22141 gcagatgcgg ctggatttga tggtattaaa gatatctaca acaagaagct taaatcaatt
    22201 tataaagcaa gcatcgttgt aactaagtca atgtttgctg cgcttgacaa agtgaaggac
    22261 aaagatgggc gctacatgct tcaaactgat gtggcttcac ctactggcta ttcatttggt
    22321 gggaaaacaa tctacaaagt agatgacaca gtgtttggaa acgaaggaga tatgaaattc
    22381 ttcatcggag atgttactga gttcgtcaaa gagtttgacc gttctcaagt atccgttaaa
    22441 tgggtgaaca atgacattta cggacaattg cttgggcttt ttatccgttt ggatattaag
    22501 aaagcagatg aagaagctgg attcttcgga acatatactg atgttgtagc ttaaggaggt
    22561 agcgtatgag ctataaagta atccgtcctt tcaaggactt ggctgatcct gaaaatcatg
    22621 actacgctgt tggcgatatc tttcctcgtg agggatatga gccaacagat agctttacca
    22681 atggcctttt gactggcgct aacactgctg gctctatctt ccttgaggtt ttgggagatg
    22741 atgaacctaa gaaaccagcg cctgaaacaa aagaagtgaa ggaagagccc gcagttgagc
    22801 aggaagaaac agttgaggaa actgctgaag agtctgctga ggaagttgag gaatagacat
    22861 ggaccaaggt cagcttttag agttgctgaa gcttaagttg ggtatttcaa ccgacttgag
    22921 agacaagccg ttaaaaaaaa tcatttcaag tgtcatcact gaattgaccg ataatctcgg
    22981 tatcgagctt gttggtgagc gtgctgacca tgaaatgttt atcgttgact atgccgctta
    23041 tcgttatgag ggtggggtgg atatgccacg tcaccttcag tggcgactgc ataatttaca
    23101 gatagcatca aagaaagagg tcaagaatgt ggaatcatga aatcacactg atctccaaga
    23161 aagtaacagg taaggataag ttactacaac caatctctga agatgttgaa gttactctct
    23221 tatgtcgcaa aaagagggtc actcgctctg aattttatca ggcgaatcag gtagggctta
    23281 aaccgagctt ggtcgttgag attcgaaatt ttgagtatga gaatcaagag cttgcgaagt
    23341 tcgaaggcaa gcaatatcat atcttgaaaa cctatcctat tgattctgaa attttagagt
    23401 tgactttgtc agaggtgttg aaatgagtaa tgaccttgct gatttgatag cgaaagagct
    23461 tgcagcttac tctgatgagg ttactgaaga agtggataag attgcagagc aagtgactga
    23521 tgagactgtg gatgagttga aagagacaag tccgaaacgg tacggaaagt atcgtagaag
    23581 ttggaaaaag aagaagttgg ccaatggctc ttttgttgtg ttcaacgcag ttgcaagtct
    23641 tactcacata cttgaaaacg ggcacctttc aagaaatggt ggtcgtgtcg ctggtatcgt
    23701 ccacatcaag ccagctgaag aaaaagcaat tcagaacttt gagaagcgaa tcaaggagat
    23761 tgggaaatga agctatcaga ctttgctgct attttggaac aggcaaactt gcctgtcact
    23821 tatcgagcgt ttaaaattgg aaacgctcct gacctacctt atctggtcta ttatgaatca
    23881 agtccagtca tcaattcagc tgacaacacg gttaatcatc agattaagag cgtgacagtt
    23941 gagctggctt ttgagagtaa ggatgaagat ttggaagaac gtctggaaga gctgtgggca
    24001 aaccacaagc tcttttttga agttcaagaa gaaacattta tcgagactga aagactctat
    24061 gtcaagtctt acacagtcta tctatactaa ggaggaatga catgactcaa gaaaataaag
    24121 taacctttgg cctagaaaat gtacatatcg cacctatcaa gacacttgca gcagatggag
    24181 ttatcactta cggcgatgtt tttcgttttc ctggagcaat agagctgata cttgatacta
    24241 aaggggaaac aacccctatc aaagcagaca acaaggatta ccatttcatg aattcaaacg
    24301 aaggatatga gggtaaactt aaaatcccac acatcattga tgaatttgcg acaaaaattc
    24361 ttggtgaaat caaggaccct caaactggtg ttatgactga aaaagcagat gcgagcttga
    24421 cagagttcgc aatgatgttc cagtttgaag gcgacaaaaa caagactcgc tatgtgatgt
    24481 actactgttt tgccagtcgc ccatctcttg gctcaaaaac taagaacggg acatcaacca
    24541 acgaacgtga acttagtttc aaagctagcc cgcgtccatt ggatacagtt gtcaagcgtt
    24601 ctatcacatc agctgatgac aaggatgcgt atgacaactg gttcaagaaa gtgtatgaac
    24661 ctactgcagt tgcagcttaa ggagaagatc tatgcgtaaa atcgttttgg ttggtgatca
    24721 ggagtatgag ttggggacca acggctatac tcctattgct tacaagcaac aatttggaaa
    24781 agattatttt caagatttgt tctcgatgtt gaaaaatcaa tcattcatga atgaattgaa
    24841 caagttggaa accgacaagg aattgacagc gactaatatt gatatttcga tgttgtcaga
    24901 ttttgacatg acctttttca accgtctttt ttggaccttt gctaaatctg caaatcctca
    24961 catcaagcct tatgaacaat tcttcatgga aatggaaatc ttcccgattc aggaagttgg
    25021 acctgtgctg atggaaatgc taaatgcgag catgacgaca aaaaagcacc agatgagtca
    25081 gaatcagcta gcgaagaaat cttcacagta gaatcctatc tatcttgctg taaagaaact
    25141 ggtctttcta ttgatgatct aaagcacatt tcaatcggaa tggctctgga ttatcagacg
    25201 gattatgtga atctacggag cgaggataag ggtggcgaac ggaaagccac gcaagctgat
    25261 tttgacagtt tttaaagaaa aatgagtgct gagagagcga ttctgagacc aagttccttg
    25321 gtctggctgc attatcagtg gtagaagttc tctcagcgct tttctatttt tttgagaaag
    25381 gaggaaatat ggcaggaaat atcaaaggta tcaaaattga aatcgatggc gacacgcaac
    25441 ccttgcagaa ggcgctgaaa aatgtcaata aggccgctac tgatgcaagt caggagttga
    25501 gacagattga caaggccttg aaatttgata cagggaacgt aacgctcctg actcagaaac
    25561 aagaggtctt acaaaagcaa gtttcgacga ccaaggagaa actggaaacc ttgagacaag
    25621 ctcagtctca ggtggaacag cagttcaaaa atggtgatat cggtgctgat cagtaccgtg
    25681 cctttcaacg cgaagtcgaa gttactcaaa acgtcctaaa aggatatgag ggtaagcttg
    25741 caaatgtgaa tcaggcgctt aatgagaatg ggagtgctac tcagaacaac aagaaccaat
    25801 taaaagagtt gcaaaatgag cagagtcaac ttgcttcaga gatgagtaag gtgacaagtt
    25861 cattcaaact gcaagaaagc gctttgggtt caaatgctag tgaagccgag agaaatgctc
    25921 ttgcccagaa aaagattggt gcccagtctg agattgtaag taaacagatt tcaaatctag
    25981 aacagcaatt ggaaatcact aaaaaagaat ttggtgagaa ctccacacaa gccaacaaga
    26041 tggaatctga gctaaatcag gctaagactg cttttaatca tctcaatgat gagatgaagg
    26101 gaacaaagtc tgctgctgat agcactcaag aaagtttaag tgaaatctca agaaatttaa
    26161 gagcagaact acttcaacag tttagtgaga agttgagtgc tatttcagaa aagcttgtgg
    26221 aagtaggaaa agaagcgtta gaagcagctg ctcaaatgca agctagtaat gctcaattta
    26281 ctaccgtttt cggagatatg gaaacccaag caagagaagc gttgaatgct attggtcagg
    26341 aaatggatat tgtcccagag cgtttacaag ggtcatttac tcagatggct tcatttgcta
    26401 aaacttcagg actagacact gcgcaagcat tggacctgac ttcccgtgta actagggcag
    26461 cggcggatgg cgcagctttt tacgacaagt ccatcgagag cgtatcagaa agcctacaat
    26521 ctttcctgaa aggaaacttt gctaacgatg ccgctcttgg aatctctgcg acagaaacga
    26581 ccaggaatgc agctgcaaat aaattgtacg gaaagtcatt caaggacttg agcgaagcgc
    26641 agaagcaatt gaccttgctt cagatggtcg aagacggaaa taaactctca ggagctcttg
    26701 gacaggctgc aagagaatca gacggcctag aaaacgtgat ggggaatctg aaacaagctg
    26761 ggaccaatgc attatctgct attggtcaac ctcttctaga aatgatgatt cctgtttttc
    26821 aaaccttggc aacgattgtg aaaggtgtgg ccgagctgtt caattccttg cctgatccag
    26881 taaaagattt tgttgttatt ttaggaacag ttgtgactgc tgtaggggtc atagccccca
    26941 tattcttaac cttgcaagcc cttgctgagt ttttaaaaat atctattgga gaaatgataa
    27001 ttgccgcatt gccaattatt ggaacagcta ttgcaattgc tgctgcagtt gctgcaatta
    27061 ttgttattgt aaaatatctc tgggaaacta acgaaggttt tcgagatgcg gtcacgaccg
    27121 tttggaatgc tatactagcc gttatcaata cagttgtttc agaaatctct gatttcatca
    27181 tgagtatatt cgggacggtt gttacttggt ggacggagaa ccaggaactt atcaggacaa
    27241 gtgctgagac tgtctggaat gccatttata cggtcatcag tacaatactg gatatacttg
    27301 gccccttgct ccaagctggc tgggataaca ttcaactgat cattacaaca acttgggaaa
    27361 tcatcaagat cgttgttgag actgcaatca atgttgtcct tggtgttatc caagcagtta
    27421 tgcagatcat tactggtgat tggtcaggag cttgggaaac tatcaaggga gtgttttcta
    27481 ctgtatggca agctattcaa agcattgttc agactatttt ctcagctatc cagagttata
    27541 tttcaaatat tctcaacggc atttcaggaa ctgtatcaaa tatctggaac agcatcaagg
    27601 acactgtctc aaatgtgtta aatgctatat ctagtactgt atcaagtgtt tgggaaggta
    27661 tcaagagtac catttcaagt gctatcaatg gtgcaaggga tgctgtttct tcagctattg
    27721 aagccatcaa aggattgttt aacttcaaca tcagctggcc acatatccca ctacctcact
    27781 tttacgtgag tggttcggcc aatccattag attggttgag tcaaggtgtt ccaagtattg
    27841 gaatcgaatg gtatgccaaa ggcggtatca tgacgaaacc aactatcttt ggaatgaatg
    27901 gtaataacat aatggttggt ggtgaagctg ggaatgaagc agtattgcca cttaacgaca
    27961 aaacgcttgg agccatcggt cgaggtatcg ctcaaactat gggtggaact tcaccaacca
    28021 tcaacattac tattactggt aacaccgtca gagaagaagc tgacatcagt cggattgctg
    28081 atgaggtggc tcagcgtatt gctgacgaat tacaacgtag gagacaattg agaggagggt
    28141 ttgcatgata aagcataatg agcttgtgat tgacggtgtg aggacatcgt cttttccatt
    28201 taagatcatt gtccatgact ctccttcaat cgctctagga gagagcaaga cagctctctt
    28261 ggagcatggt ggtgtcagtg gggcaatcgt tcagacaaac aagcataggg aactggtcaa
    28321 gaaaacttat acgatttact tggtcaaacc tactgaagaa cagatgaacc aatttatgag
    28381 tctgtttatc cgtgaaaagt tctggctaga gagtgagcga gtcaaaacaa ctcgtctttg
    28441 gtgctataag gtcaatgtga gcgaccttga agaagtgcaa cctggtcttt acatgaccaa
    28501 agcaaccttc acttgccatc ctacaaaata ctttaaagac tccgatacac agagattgac
    28561 aagaagtggg actttgaccg ttcaaggttc tgctcttgcc tttcctaaaa tcacaatcgt
    28621 tggtcagagc gcttctgaaa cttcatttac aattgctggt caggtcattc gtcttgaacg
    28681 actcactgag tcgcttgtga tggttaataa tcctgacaat ccaagtttta aaacaacaac
    28741 agggaagcca gtcaaatggt caggggattt tatcacagtt gatccagcga aagtgaagaa
    28801 tattggggtt gttttaggcc caggtattca atcgattgaa atcgaaacgg tttgggggtg
    28861 ggcataattg ctttatctac ttaataaaga tgtaagaacc gttcgatgga acggggagcc
    28921 acttcatgaa gcgacttcgg cgattgtgaa agaaactatg aatggcgatt ttaccttgac
    28981 tgtgaaatat cctatttctg attctggtat ttaccagctc atccaagaag atatgctgat
    29041 aaaggctccg actcctgtct taggagcgca gctatttcgt atcaagaaac ctgttgagca
    29101 caatgaccat ctggaaatca cagcctatca catttcagat gatgtgatgc aacgatctat
    29161 cacacaaatg agtgtgacta gtcagagttg tggcatggct cttgctcgca tggtacaaaa
    29221 caccaaaacg gctcttggag acttctcatt caacagtgat atccaggacc gtaggacatt
    29281 caacacgact gagacagaaa ccctgtactc tgtattgctg gacggcaagc atagtatcgc
    29341 cggaacgtgg gaaggcgagc tggtgcgtga taactttgcg atgactgtaa agaagagtcg
    29401 tggtgagaat cgtggtgttg ttattacaac acataagaat ctgaaggact accaacgaac
    29461 caaaaacagt caaaatgttg ttacaaggat tcatgctcga tccacattta agcctgaagg
    29521 tgctgaaaag gaaacaacta tcagagtgac tgttgatagt cctcttatta attcttatcc
    29581 ttatataaac gaaaaagagt atgagaacaa caacgcaaaa tccgttgaag agttgcagaa
    29641 gtgggcacag tctaagttct caaatgaggg aattgacaag gtctctgatg ctatcaaaat
    29701 tgaagcttat gaacttgatg ggcaagtggt ccatatgggc gacacggtca atctcaagag
    29761 ctggaaacat aatgtcgatg cattcaagaa agctattgct tatgagttcg atgccttaaa
    29821 agaagaatac atctctctga ctttcgatga taaggcaggt attggtggtt ctagatcttc
    29881 tggtggccta tctagcgcag ccgatgcaat tcttggagtg acagaatctg cacaagaaat
    29941 cgcccttgaa aaggctcttc aaaatgctga cttagacttt gatcataagg ctggattgct
    30001 tagacaggaa atttctgacg atattgaact ggccaaagcc agagctgaag aagtcaagag
    30061 agaactgtct gacactatca atcagcgatt taatagcttt gacaacgggc cattgaaaga
    30121 aactaagcgc aaggctgagg aagctttgcg aaatgctggc gcaagtaccc tgcttgcaca
    30181 ggaagctaag cggattgggc tggattctgt cgctagactt gaagcgttta agtcgcagac
    30241 tacgagcgca caaacggctc tgtcgggtga cttggacgct ctgaaacgga ctatcgcgaa
    30301 tgatattcga ccgaagcaag cacaggctga agctgagatt gccaagcaag ctgaagcact
    30361 tagccggact aaaaatgaac tggctggcgc aagtacccta cttgcacagg aagctaagcg
    30421 gattgagctg gattctgttg ctagacttga agcgtttaag tcgcagacta cgagcgctca
    30481 gacggctttg tcaggtgact tggatgttct aaaacaaact atcgcaaacg atattcgacc
    30541 gaagcaagca caggctgaag ctgagattgc caagcaagtt gaagcactta gccggactaa
    30601 gaatgaactg gctggcgtga agtcagcgca agcgacgtat aaggagacga cgactcgtag
    30661 actgtcagaa ctgaccaact tggccaatgg taaagccagc aagtcagaac ttacgcaaac
    30721 agctgaggag ctggctagtc ggattgcgag tgtgcaggca ggtagttcac ggaattactt
    30781 caggaattca cgttcaagaa cgttcacaac aggaggtcaa gcggtatacg actatcgaac
    30841 attcatagtt cctgatttct ggaagaacag tgacaggttc aagcgtgatt atgttcgcat
    30901 atcttttgat gtgactttcc ctgtcgccct agtaaatgac atgcctgcta tggtgcattt
    30961 tagtgctcat ccatggtatg cctacagaaa cttaattttt aaaggtggaa ctgtcgaacg
    31021 ccaacatttt gagtttacga ttgacttgtc tagttcttct gaggactatc agactaataa
    31081 tgtgttcatt cgttttggta ctaattatgg atttcctgct ggtctgcagg tcgtcattga
    31141 gaacgctatg ttatcggttg gtaattattt tccagcctat caaccagcgt atgaagacca
    31201 agaagaccgt gtctcagtag tcgaatccaa ctttaaacag cgtgctgatt cactcgacgc
    31261 tggtgtaaga agcttgactg aaggccttag aaccaaagcg gatatcagct cactcaatgt
    31321 gactgctgaa aatatccggc aatctgtgaa gagccttgag acagatacgc agaacaagct
    31381 aaatcagaag ttgagtcagg ctgaatttga ggtgcgagcc ggctctatcc gtcaggaaat
    31441 cctgaacgca accaaggata aagccagcaa gtcagaactc acgcagacag ctgaggagct
    31501 ggctagtcgg attgcgagtg tgcaggcatc cggtcgaaat ctattcttga actcactatt
    31561 caagcaggat attccaaaaa caggaatttg gacaacgagt acatatacgg ctactatcga
    31621 tagcgaaagt aagtatcttg gacacaaggc tcttaaaatt ataggtttga atccatctgg
    31681 ccgtgatgga ggtaatccca aggttactta tccagctctg ggtcaattcg ggaaagtaat
    31741 tcccggaagt acgactaatc aagatgtaac cattagtttt tatgctaagg caaataaaaa
    31801 tggaataatg ctaagatctc gattagggaa tatcggatat aaaactggaa atgtgacatt
    31861 gtcgacagaa attaagcgat atgttgtcca tattccaaaa ggttggacaa acgaatccaa
    31921 gcagaccaca aatgaatggt tgttcaattt caaccaggaa ggaaccattt ggatttggat
    31981 gccgaagttt gaaataagcg atgtagatac ttcttattca gaagctcctg aagatataga
    32041 aggtcagatt tcaacagttg aatcgacctt caaacaacga gccaactaac tcgacgctgg
    32101 tgtaagccgt ctgactgaag gccttagaac caaagtggat atcagctcac tcaacgtgac
    32161 tgctgaaaat attaggcagt cggtgaagag tcttgagaca gacacgcaga acaagctaaa
    32221 tcagaagttg agtcaggctg aatttgaggt gcgagcaagc ggaatccgtc aggaaatcct
    32281 gaatgcaacc aaggacaagg ctgacaagac cttggtaaca gcggaggctg ggaagttgag
    32341 agaggagctg acaagtctgt cggttggcga gaatctgttt gtaaactcag attttaaaaa
    32401 tctgagggat aacggacagc gttatacagc taacggcaag acctatcaaa acatgattgc
    32461 gccttactgg tacaatccgt acaatgctgg tattccaaat gctcaaaata tccagcatgg
    32521 gtattttgac acggaaacgt ttagtgatac tgtctttgcc tttaacgaga gcgacggctc
    32581 ccgacactgg aaagcattgt caactgattt taaaatcgga gtcatctcag ctggagagta
    32641 ttacttttca gctgatttgt atgcgacaga tttaggcact catatcaaat ttggatttta
    32701 ctatcacaac tcgactggca aactcaattt ttacgcaggt cgaacaaaaa ttgaagttac
    32761 tgaaaaaggc cgttggacta ggctaggaat tgacttaaag gtcaatgatg acattgacct
    32821 gacaaaaaaa gtccaatttt acatttacgg ctataatttt gctagcaact caattttgta
    32881 ccttaaaaag cctaaagtct cgaaaggtcg cttaaaaagc gattggagcc cagctctgga
    32941 agacaccgaa ggcctcatca ctgaggctaa ggcaaccttt gagcggacag ctcagggctt
    33001 gcgaaccgac ttatcagcta ttcaggaata tgtaaataaa gacggtcagc gacaggaagc
    33061 cctacagcgc tatactcgtg aggagagcac gagacaagcg acagcagtcc gtgagctggt
    33121 caatcgtgat ttcgttggta aggctactta tcaagaagat gttaagggta tcaatcagag
    33181 gattgaagct gttaaaacta gtgcgaataa agacatcgct agtcaaatcg ctagctatcg
    33241 tcaatctgta gatggtaagt tcacggatat ttcaagtcag ataactactt ataagcaaga
    33301 tgtgggcggt caaatcagtg gtctatcaaa tagacttaca agcagtgagc aaggaaccac
    33361 tactcagatt tcaaatattt caaatcggat aaacagtaat aaacaaggca cagataatca
    33421 gatttcaaat ttaaagactc aggtcgctac aaacaaggat aatgctgaac gacaaatggg
    33481 tagaatatct gatcaggttt ctgcaaacaa agcgaatgct gatagtcaat ttgcgaatgt
    33541 gaccaatcaa ctagcgcgaa aagtagagac tactgacttc cagcgtgtta aggaaaccag
    33601 taaactttac gagcggattt tgggcaatac tgaaaatgga attgcggata aggttgctcg
    33661 catggctctg accaatcaac tgtttcaggt tgaggttggg aaatatagtg taagcggccc
    33721 taacctcatt aagaatagtg attttaaaaa tggtacgaat gaatggggct caactcaaaa
    33781 tttaggaaga ttggttaagc atagctttta tcacaacggg cagaaagacc ttatgcgttt
    33841 aagtaatgca actaaaaacg aaaacttttt gtatagtcac cgttttaatc ttgaacgaaa
    33901 tactgactat gtactgaatt ttagaggatt taacaacagt gctctcgcaa gctatgatgt
    33961 ttatattttg ggacgaagag caggcgagag cgatggattc acaatcgtta agaaagttgt
    34021 tagcagcaag aaactatcta cctctagatg cgaagatgtc tcagtaactt ttaattccgg
    34081 agaaatggat aatgcttaca ttcgttttga taacaatggc tcatcatcag gaacagctga
    34141 tttgtatatt acagaagttg acttgtacaa aggttataaa cctagaacat ggcaaccaca
    34201 tccagaagat gcagtcgcag atgcgaataa gaagcttgaa gccacgcaaa caaaaatgac
    34261 tcaactagct ggctcatggg tagttgaaaa catcaactcg gctggagata tcatctctgg
    34321 aatcaatctt ggcgccaatg gacataaccg ctttgttggg aaattgaccc acatcactgg
    34381 agagaccctg attgacagag cagtcatcaa gtctgccatg gttgataagc tcaaaacggc
    34441 caattttgaa gctggttcgg tcacgactac gatattagac gctgaagcgg tcacggctga
    34501 taaagtgaga tttgatgctg cgtttattag aaaaatgatt gcaaatgacg cttttattga
    34561 ccaactgaca tctaaacgta tcttctctac taaggttgag tccgtcattt ctagttcaac
    34621 cttcctagaa gcctatcaag gccgaatcgg tggatttaca cttggtcaat ttgatcaggg
    34681 tggcggtcgc tggatttcag gtgtcaatca gttctctgtt ggtatgggga atggtgccgg
    34741 gcatggggtg ccggacagcc ttctgggcga actggggaaa taattggaac tatgccggac
    34801 ctaaagcatg gaacgtcaat actgatggga aaatgtactg taggaatgaa gtcggttttt
    34861 atgatcaagt ggatttttcg aattcatcga gagcaaactt ctatgggaat actacttttt
    34921 ctcgttctcc tgtgttttca aatggtatcg aacttggaag taaagatgtg cttggtgatg
    34981 gttggaatcc caaaggcgga aggaatgcgg ttgtttggtg gaatcaggtc ggtagcggta
    35041 gcgtgaagta ttggatggaa caaaaatcag acagacgctt aaaagagaac atcacagata
    35101 cagctgtgaa agccttggac aaaatcaaca gattaagaat ggttgcattt gatttcatcg
    35161 aaaataagaa acacgaggag attggtctaa tagctcaaga ggctgaaacc atcgttccaa
    35221 gaattgtctc acgagatcct gagaatccag atggctatct acatatcgac tataccgctt
    35281 tagttcctta cttaatcaag gctattcaag aattaaatca aaaaatagaa aaaatggaga
    35341 aaacaatagc atgaataaca acatgttgac caatatcgca cttaaagcaa ttcaggagtt
    35401 tgctcttgaa aatagaaaac gaacacacag attggagaac ttagaaaatg aacacagaac
    35461 agcttaacca agccttacaa atgacaattc gtgaaatgtc aacaacttca acagattcga
    35521 tgattgcaag taatttcttg agtattcagt tgaatgagca aagggaagag aatcaaagac
    35581 ttcaagcacg agtggatgag ctggaagctc tgcttgatga acaaactaaa ccagcagaca
    35641 aaggagaata gacatggcag aaacaattca aaacacagat aacttactag accttacaaa
    35701 aatcacagaa ccatttgatc ttgcgagcgc tttgcgctac atgaaagaaa atggagagtt
    35761 cattcgttgc aagaatgtaa gcgatgactt ctatatgtat cgtgatgttc aaaaacgtcc
    35821 tgtgatcgta aatggccgtc gccaattcaa ggatgttgaa actgtttggg cgttcaacca
    35881 gtgggggggg tacaatcaca acaatcaacg tagccgttct gttgaatcat gaattctata
    35941 tcatgaaatt tgatgcagag ggcaatcctg actggacgga tccaacggta aaacctaaag
    36001 aataggaggt gtgtatgcaa attgaatttt tcaattttct aagaagtgtc gtacagactg
    36061 aagatggttt ggtcttgtac gctctagcac tgattgtctc aatggaaatc attgattttg
    36121 tgacagggac gattgcggcg attatcaatc ctgacatcga gtacaagagc aaaatcggca
    36181 ttaacgggct ccttcgtaag atttcagggg ttctcttact gatgatcctc attccggcgt
    36241 ccgttttgtt gcctgaaaag acaggttttg tattcttgca ctcaatctat ctcgggtaca
    36301 tcgcatttac ttttcaatct ctcattgaaa attaccgcaa attaaaagga aatgttactc
    36361 tttttcagcc gattgtaaaa gtatttcagc gattacttga aaaagatgat gatacgaaaa
    36421 aaggagaata acaaatgcaa caaattactg aaatcattac taatggagca atcagcatcc
    36481 tagtcatttt ggcaggggtg gtagttaggg tagtcaagga atacctcgtc aaaaaaggtg
    36541 gagaaaagac tatcaagatt gttgaaatct tggccaagaa cgcagttaat gccgtggagc
    36601 aggtagctgc tgaaactggc tacaagggag atgaaaaact ggcacaggct cgcgctaaaa
    36661 ttcgtgcaga gctgaccaaa tataatatca gtatgacgga caaggacttg gacacctttg
    36721 tggagtcagc cgtgaagcag atgaacgacg cttggaaagg acaagagtaa tggatatcga
    36781 tagaaacaga ctacgtacag gcttgcccca ggttggggtg cagccttatc gacaagtaca
    36841 tgctcactca acaggcaacc gtaactcaac cgctcaaaat gaagctgatt accactatag
    36901 aaaggaccct gaactagggt tcttttcaca tgttgtcgga aacggccgca tcatgcaggt
    36961 aggacctgtg aacaacggaa gttgggatgt tgggggcggt tggaatgctg agagttatgc
    37021 agcggttgaa ctgattgaaa gccattcaac taaggaagag tttatggctg actatcgcct
    37081 ctatatcgaa ttgctacgca atctagcaga tgaagcaggt ttgccgaaaa cgcttgatac
    37141 agggagttta gctggaatta aaacgcacga gtattgcacg aataaccaac caaacaacca
    37201 ctcagaccac gttgaccctt atccatatct tgcaagttgg ggcattagcc gtgagcagtt
    37261 taagcaagac atcgaaaacg gcttgagcgc tgcgacaggc tggcagaaaa atggtactgg
    37321 ctactggtac gtacactcag acggctctta tccaaaagac aagtttgaga aaatcaacgg
    37381 tacctggtat tatttcgatg gctcaggcta tatgctttca gaccgctgga agaagcacac
    37441 agacggtaat tggtactact ttgaccaatc aggcgaaatg gccacaggct ggaagaaaat
    37501 cgctgagaag tggtactatt ttgatgtaga aggtgccatg aagacaggct gggtcaagta
    37561 caaggacaca tggtactact tagacgctaa agaaggcgcc atggtatcaa atgcctttat
    37621 ccagtcagcg gacggaacag gttggtacta cctcaaacca gacggaacac tggcagataa
    37681 gccagacttc acagtagagc cagatggctt gattacagtt aaataaatag aaaggaaact
    37741 ttctaaattg ttctttcacc gcaggctcag gcttgcggtt tttttgtttg tctgaatcaa
    37801 gaaagcgtct aaccaaccga caccaatgtc ggtagcaaaa taaatggttt gcctaaaaaa
    37861 ttagagttaa gttctatttt tcaaaaacac gcattttgaa cgattagaaa cagaaatcta
    37921 aatcctattg ttcaaaaaaa cgtttacatg aaaaataggg aggtgttttg ttaaaaataa
    37981 aaacagtgat cgaaatcact gcttatcagt tgtagcaaat tcataaagtt tttctgctgt
    38041 gagaagtgcc attttgtcca tgcttgtttt tccttttctg aggtcagaaa cagtagtcca
    38101 tggaactcca gcaccttggg aaatagcaga tgtagacatc gaactgtcta acaattcttg
    38161 aataacttct ctcatcttat ttgtcctttt tattttttag ataaatatat acattgattg
    38221 caattataaa aatagctatt gcactaacca ttgcatttcc tctttccatt tgataaaata
    38281 gaggtgtaag gggctttcgc cccaacctct tagcgtttac ctttttcttt gacgggattt
    38341 cggtttacgc tttttgtttt gccttgcgac cgttattgcg gtcactagac ttgcgatagc
    38401 agttaccgtt tcaggaatat tgtctattgc cttttcaagt aacctgagcc aatcttcttt
    38461 gttcaacttc atcaccccct ttccttatct tgattatatt atatcacggt acaccgagaa
    38521 agtcaagcgt tttgatgaag ttttttttaa ttttttcaaa aaaaaataga ccttgtccag
    38581 aggtcgggga gttggagggg gcaccctcca aaagcattga tttaataagg ttttatttta
    38641 cctttttcat aataatctcc ctatagagtc accgcattcg gtggcttttt ttgtgttggg
    38701 attcatgata taataataaa atcgataagt aggaaaagag aaaagagatg tattatacgc
    38761 ttgaagaaaa agaagtcttt atgagggagg ctttgagaga ggctgagatt gctcttgaac
    38821 acgatgaaat tccaattggt tgtgtgattg tcaaagatgg ggaaatcatt ggtcgtgggc
    38881 ataatgcgcg tgaggaatta cagcgagcgg ttatgcatgc ggaaattatg gctatagagg
    38941 atgcgaactt gagtgaggag agctggcgct tgctggattg cacacttttt gtgaccattg
    39001 aaccttgtgt catgtgtagt ggagcgattg ggcttgcccg cattccaaat gtggtctatg
    39061 gggctaaaaa ccagaaattt ggcgctgctg ggagtttgta caatatcttg acagatgagc
    39121 gtctcaatca tcgtgtggag gttgaaatag gaattttgga agatgaatgc gcagctatca
    39181 tgcaggattt ttttagaaat agacggaaaa aataattttg cttttaaaat gaataggaat
    39241 gtgatataat aaatagtgga gcaacagttc tgcgtgaagc gggtcagggg aggaatccag
    39301 cagccctaag cgatttgaat tgtgtgctct ttttttcgtg ctttttccga ataaataaga
    39361 tagaataatc tagaataaat gataatagaa aagagaaaat tatgaaaatt cgtggttttg
    39421 aattggtttc gagttttaca gatgaaaatt tattgcccaa gcgtgagaca gcgcatgcgg
    39481 ctggttacga cttaaaggtt gctgtgcgta cagttgttgc gccaggagag attgtcttgg
    39541 ttccgacagg ggttaaggct tatatgcagc cgactgaggt tctctacctc tatgatcgtt
    39601 cttcaaatcc tcgtaagaag ggcttggttt taattaactc agttggggtc attgatgggg
    39661 attattatgg aaatcctgga aatgaagggc atatttttgc gcagatgaag aatatcacag
    39721 atcaagaggt tgttcttgaa gttggggaac gtattgtcca ggctgttttt gctactttct
    39781 taattgcaga tggagatgca gctgatggcg ttcgaactgg tggatttgga tcgacagggc
    39841 actagaatga agattatctt tgtacgtcat ggggagccag attaccgtga gttagaggag
    39901 cgttcttata taggatttgg gatagatttg gcacccttgt ctgagatggg acggcagcaa
    39961 gtccagaaat tgagcaaaaa tcctttactc tcgtcagctg aaataatcgt atcttctgca
    40021 gtcacaagag ctttagaaac ggcttcgtat gtggtctgtg ctacgggtct tcctttaaga
    40081 gtagagcctt tattacatga atggcaggtc tataaaacag gaatagaaaa ctttgaaaca
    40141 gctagaagac tgtttttaga aaacaagggg gagttgcttc ctaatagtcc tattcaatat
    40201 gagacagcta cggaaatgaa gtctcggttt ctagaatgta tgtctaagta tcgagaacat
    40261 cagactgtgg tagttgttgc tcatcgaatg ctcatgcgcc agtttgtgcc aaatgagaag
    40321 attgattttt gccaagtgat tgagtgtgag ttagagatat agaaagaggt ttgtcatcgc
    40381 aaagaaaaaa gcgacatttg tatgtcaaaa ttgtgggtat aattccccta aatatctggg
    40441 acgttgcccc aactgtgggt cttggtcttc ttttgtggaa gaggttgagg ttgccgaggt
    40501 caagaatgcg cgtgtgtcct tgacaggtga gaaaaccaag cccatgaaac tagctgaggt
    40561 gacttccatc aatgtcaatc gaaccaagac ggagatggag gaattcaacc gtgtgcttgg
    40621 aggcggagtg gtaccaggaa gtctcgtcct catcggtggg gatcctggga ttgggaaatc
    40681 aactcttctc ctacaagtct caacccagtt gtcccaagtg gggacagttc tctatgtcag
    40741 tggggaggag tctgcccagc agattaaatt acgtgcagag cgcttaggtg atattgatag
    40801 tgagttttat ctctatgcag agaccaatat gcagagtgtt cgtgcagaag tggagcgtat
    40861 ccagccagac ttcttcatta ttgactctat ccagaccatt atgtctcctg agatttcagg
    40921 ggtgcagggg tctgtttctc aggtgcgtga agtgaccgct gaactcatgc agttggccaa
    40981 gaccaataac attgccatct ttatcgtagg tcatgtgacc aaagaaggaa ccttggctgg
    41041 gcctcgtatg ttggagcata tggtggatac ggtgctttac tttgaagggg agcgtcacca
    41101 cacctttcgt attttgagag cggtcaaaaa tcgttttggt tccactaatg agattgggat
    41161 ttttgagatg cagtcgggcg gcttggttga ggtactcaat ccgagtcaag ttttcctaga
    41221 agagcgtttg gatggggcga ctggttcctc catcgttgta accatggaag ggacgcgtcc
    41281 gattttggcg gaggttcagg ctttagtaac accgaccatg tttggaaatg ccaagcgtac
    41341 tacgacagga cttgatttta accgtgctag cttgattatg gctgttttgg aaaaacgggc
    41401 agggcttctc ttgcaaaatc aggatgccta tctcaaatct gctggtggtg ttaaattgga
    41461 tgaacctgcc attgacttgg ctgttgcagt tgctattgct tcgagctaca aagacaaacc
    41521 aactaatcct caggaatgtt ttgtcggaga actgggcttg acaggagaga ttcggcgcgt
    41581 gaatcgtatt gagcaacgca tcaacgaagc tgctaaactg ggctttacta agatttatgt
    41641 acctaagaat tccttgacag gaatcactct gcctaaggaa attcaggtca ttggcgtgac
    41701 aacgattcag gaagttttga aaaaggtctt tgcataatcc gtgacaaatt ctcttaaaaa
    41761 tgataagata ggagaaatat ttgactatca aattttcaag gagggaatcg tgtcgtattt
    41821 tgaacagttt atgcaagcca atcaggctta tgttgcccta catgggcagt taaatctgcc
    41881 acttaaaccc aaaacaagag tagctattgt gacctgtatg gactctcgtc tgcacgttgc
    41941 gcaagctctg ggcttggcac ttggggatgc tcatatcttg cggaatgcag gtggtcgagt
    42001 gactgaagac atgattcgtt cgctagttat ttcccagcaa caaatgggga caagagagat
    42061 tgtggtattg caccatacag actgtggtgc tcagaccttt gaaaatgaac cttttcagga
    42121 gtatttaaaa gaggaattag gtgtagatgt gtcagaccag gatttcttgc ccttccaaga
    42181 tatagaagag agtgtacgcg aggatatgca actgcttatc gagtctcccc taataccaga
    42241 cgatgtcatt atctctggtg ccatttacaa tgttgataca ggaagtatga cagtcgtaga
    42301 attataaata cttcatttag aaagaaagtg tatgaagaaa agcagtattt tattgctatg
    42361 tattggttta cagtatgaaa ccatctacta tacggacggt tcgaggtcag gtgcggaata
    42421 tggactaatg ggaatttcta tctttctagc tctcttttac atgattccgg ctctttattt
    42481 tctcttccgt attgggaaaa atgggaattg ccaaagaagg ttttgattct gtctttattg
    42541 ggagggatgt tcctttcagg ctggttgtct agttttgcta atacttatat ccatgattta
    42601 ctgggggttc ttttcccaga tagtacattt ttaaatgcct ttgaaagtgc tattgtggct
    42661 cctttggtag aagaaccctt gaaattattg ccacttgttt ttgttttggc tttgattcct
    42721 gtgcgaaaat taaaatcttt gtttttactt ggaattgctt ccggtttggg attccaaatg
    42781 attgaggata ttggttatat tcgtacggat ttgccagagg gctttgactt tactatttcg
    42841 cgaattttag agcgtatcat ctcaggaatt gcctctcact ggactttttc aggtctagct
    42901 gtagtaggtg tttacttgct ttacagagcc tataaaggac agaaggttgg caagaaacag
    42961 ggccttattt ttctaggttt agccttggga actcacttct tgtttaactc tccttttgtg
    43021 gagttggaaa cagagttgcc tttagcgatt ccagtggtta cggctattgc tctctatggt
    43081 ttttatcatg cttattgctt tgttgagaaa cacaatgagt tgatgaccta gaatattttt
    43141 caaaagaatg atgcaagggt acaaatatgg tgcccttctt ttatttttga ttgaaaaata
    43201 gtgcaaaaag cgctacaatg gtagatggaa aatcttgtga aaggcacaag ttatacatat
    43261 ataccggagg aaatcatgtc tttttctgat ttaaagctgt ttgccctttc ttctaataaa
    43321 gaattggcag aacgtgtggc gcaggagatt gggatagagt tggggaaatc aagtgttcgc
    43381 caattttcag atggagagat tcaggtcaac attgaagaat caatccgtgg gaaacacgtc
    43441 tttatcttac aatcaactag ttcgcctgta aatgacaatc tgcttgaaat tttgattatg
    43501 gtagatgctt tgaagcgtgc gagtgcagaa tctgtcaatg ttgtcatgcc ttactatggg
    43561 tatgcacgtc aggatagaaa ggcgagagcg cgtgagccaa tcacttcaaa acttgtcgca
    43621 aatatgcttg aagtagctgg agtggatcgt ttattgacca tcgacttgca tgctgcgcaa
    43681 attcaaggat tctttgatat tcctgtggat catttgatgg gtgctcctct gattgcagat
    43741 tattttgagc gtcgtggtat ggttggttct gactatgtgg ttgtcagccc ggaccatgga
    43801 ggggtgactc gtgctcgtaa gttggcagaa tttttgaaaa catctatcgc tattattgat
    43861 aaacgtcgta gcgttgataa gatgaatact agtgaagtta tgaatatcat cggtaaggtt
    43921 gaaggcaaga cttgtatctt gattgatgat atgattgata ccgctggaac gatttgtcat
    43981 gcggcagatg ctcttgcgga agctggtgct gttgaagtct atgcaagctg tacgcaccca
    44041 gttctttctg gtcctgctac ggacaatatc caaaaatcag ctattaagaa attggttgtt
    44101 ttggatacca tctatctgcc agaagagcgt ttgattgata agattgagca gatttcaatc
    44161 gctcatctcc taggggatgc tatcgtacgt attcatgaaa aacgaccact ttctccactt
    44221 ttcgatattg agaaaaaaat ttaatgacca agcctgagat gattctcagg ttttttcgtc
    44281 ttttttccga ataaatagat agtagcggtg aatctagtaa agctagattt aaaactgtga
    44341 tataataaaa agagaagaaa tatgactgta cgtcat
//
LOCUS       DAVBNO010000012        43719 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVBNO010000012 DAVBNO010000000
VERSION     DAVBNO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43719
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.012"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            131..412
                     /locus_tag="VQ846_000719"
                     /pseudo
     CDS             131..412
                     /locus_tag="VQ846_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(537..740)
                     /locus_tag="VQ846_000720"
     CDS             complement(537..740)
                     /locus_tag="VQ846_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3075345.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(733..1815)
                     /locus_tag="VQ846_000721"
     CDS             complement(733..1815)
                     /locus_tag="VQ846_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET3075346.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1941..2723)
                     /locus_tag="VQ846_000722"
     CDS             complement(1941..2723)
                     /locus_tag="VQ846_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET3075347.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2723..4066)
                     /locus_tag="VQ846_000723"
     CDS             complement(2723..4066)
                     /locus_tag="VQ846_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET3075348.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKKCTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(4165..5481)
                     /locus_tag="VQ846_000724"
     CDS             complement(4165..5481)
                     /locus_tag="VQ846_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET3075349.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVNIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYLEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5675..6901
                     /locus_tag="VQ846_000725"
     CDS             5675..6901
                     /locus_tag="VQ846_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HET3075350.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            7100..8674
                     /locus_tag="VQ846_000726"
     CDS             7100..8674
                     /locus_tag="VQ846_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET3075351.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQNIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8939..9727
                     /gene="codY"
                     /locus_tag="VQ846_000727"
     CDS             8939..9727
                     /gene="codY"
                     /locus_tag="VQ846_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET3075352.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9727..10302
                     /locus_tag="VQ846_000728"
     CDS             9727..10302
                     /locus_tag="VQ846_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET3075353.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGNFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10384..10469
                     /locus_tag="VQ846_000729"
     tRNA            10384..10469
                     /locus_tag="VQ846_000729"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10418..10420,aa:Leu,seq:aag)
     gene            10752..11882
                     /gene="ugpC"
                     /locus_tag="VQ846_000730"
     CDS             10752..11882
                     /gene="ugpC"
                     /locus_tag="VQ846_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET3075354.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            12383..13141
                     /locus_tag="VQ846_000731"
     CDS             12383..13141
                     /locus_tag="VQ846_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET3075355.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            13228..13740
                     /locus_tag="VQ846_000732"
     CDS             13228..13740
                     /locus_tag="VQ846_000732"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET3075356.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13922..14866
                     /locus_tag="VQ846_000733"
     CDS             13922..14866
                     /locus_tag="VQ846_000733"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET3075357.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            14875..15552
                     /locus_tag="VQ846_000734"
     CDS             14875..15552
                     /locus_tag="VQ846_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET3075358.1"
                     /translation="MTYLDAFKSGILVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            15650..16408
                     /locus_tag="VQ846_000735"
     CDS             15650..16408
                     /locus_tag="VQ846_000735"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET3075359.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16446..17951
                     /gene="lytC"
                     /locus_tag="VQ846_000736"
     CDS             16446..17951
                     /gene="lytC"
                     /locus_tag="VQ846_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HET3075360.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKVIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18448..18966
                     /locus_tag="VQ846_000737"
     CDS             18448..18966
                     /locus_tag="VQ846_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET3075361.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEASEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            19096..19602
                     /locus_tag="VQ846_000738"
     CDS             19096..19602
                     /locus_tag="VQ846_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET3075362.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEL"
     gene            19804..21036
                     /gene="clpX"
                     /locus_tag="VQ846_000739"
     CDS             19804..21036
                     /gene="clpX"
                     /locus_tag="VQ846_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET3075363.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            21045..21632
                     /locus_tag="VQ846_000740"
     CDS             21045..21632
                     /locus_tag="VQ846_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET3075364.1"
                     /translation="MELNIHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            21643..22023
                     /locus_tag="VQ846_000741"
     CDS             21643..22023
                     /locus_tag="VQ846_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET3075365.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            22075..22965
                     /gene="rapZ"
                     /locus_tag="VQ846_000742"
     CDS             22075..22965
                     /gene="rapZ"
                     /locus_tag="VQ846_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET3075366.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            22962..23939
                     /locus_tag="VQ846_000743"
     CDS             22962..23939
                     /locus_tag="VQ846_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET3075367.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVVGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQKYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            23936..24847
                     /gene="whiA"
                     /locus_tag="VQ846_000744"
     CDS             23936..24847
                     /gene="whiA"
                     /locus_tag="VQ846_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET3075368.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(24881..25849)
                     /locus_tag="VQ846_000745"
     CDS             complement(24881..25849)
                     /locus_tag="VQ846_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET3075369.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            25985..26842
                     /locus_tag="VQ846_000746"
     CDS             25985..26842
                     /locus_tag="VQ846_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET3075370.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            26829..27608
                     /locus_tag="VQ846_000747"
     CDS             26829..27608
                     /locus_tag="VQ846_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET3075371.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27632..28984
                     /locus_tag="VQ846_000748"
     CDS             27632..28984
                     /locus_tag="VQ846_000748"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET3075372.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            29058..29432
                     /locus_tag="VQ846_000749"
     CDS             29058..29432
                     /locus_tag="VQ846_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET3075373.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPNELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29434..30282
                     /locus_tag="VQ846_000750"
     CDS             29434..30282
                     /locus_tag="VQ846_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET3075374.1"
                     /translation="MKLAVFTDSSAYLSAETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30559..31326
                     /locus_tag="VQ846_000751"
     CDS             30559..31326
                     /locus_tag="VQ846_000751"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET3075375.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLACFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRTQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEIRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            31338..32522
                     /locus_tag="VQ846_000752"
     CDS             31338..32522
                     /locus_tag="VQ846_000752"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET3075376.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLENISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            32519..34390
                     /locus_tag="VQ846_000753"
     CDS             32519..34390
                     /locus_tag="VQ846_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET3075377.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTILSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELVWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34533..35717)
                     /gene="mntE"
                     /locus_tag="VQ846_000754"
     CDS             complement(34533..35717)
                     /gene="mntE"
                     /locus_tag="VQ846_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET3075378.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLATGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLENRF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            36021..38717
                     /locus_tag="VQ846_000755"
     CDS             36021..38717
                     /locus_tag="VQ846_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET3075379.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLVEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILAIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            38730..39521
                     /gene="yghU"
                     /locus_tag="VQ846_000756"
     CDS             38730..39521
                     /gene="yghU"
                     /locus_tag="VQ846_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET3075380.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            39637..40047
                     /locus_tag="VQ846_000757"
     CDS             39637..40047
                     /locus_tag="VQ846_000757"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET3075381.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            40059..41654
                     /locus_tag="VQ846_000758"
     CDS             40059..41654
                     /locus_tag="VQ846_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075382.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            41721..41885
                     /locus_tag="VQ846_000759"
     CDS             41721..41885
                     /locus_tag="VQ846_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET3075383.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            41964..42692
                     /gene="yaaA"
                     /locus_tag="VQ846_000760"
     CDS             41964..42692
                     /gene="yaaA"
                     /locus_tag="VQ846_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET3075384.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPVLSPMAPHRLDFLMKLKVAGKTLKSHWKAAYDETLKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTLKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(42969..43517)
                     /locus_tag="VQ846_000761"
     CDS             complement(42969..43517)
                     /locus_tag="VQ846_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET3075385.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
BASE COUNT        12874 a         8170 c         9946 g        12729 t
ORIGIN      
        1 aagaaagaca gaaattgttc aagaattaat gactgagttt tcgttagttc ttcttctaaa
       61 agccattaaa ctagctcgtt cgacctacta ctatcacttg aaacagctag acaaaacaga
      121 taaggactaa ggctggcaat accaacacga ttcttatcat cggttcctag agagtaaggg
      181 aattcaagca tctatgtcac gcaagggaaa cagccaagac aacggtatga tggagtcctt
      241 ctttggcatt ttaaaatccg aaatgtttta tggctatgag aaaacattta aatcacttaa
      301 tcaattggaa caagccatta tagactatat tgattattac aacaataaga aaattaagtt
      361 aaaactaaaa ggacttagcc ctgtgcaata cagaactaaa tccttcggat aaattaattg
      421 tctaactttt tgggggcagt acacaatacc tgatagtatg cgttttattg tgggaagatt
      481 ttcctcattt tctagtgaaa ttgaactttt tctaacatct tttgatagat gacttcttaa
      541 tcaattatca tttccattgt tgtttcttgg ggtttcatcc cttgtctttg ataaaagcgg
      601 agagcaccct cgttatcatt ccagacactt agggttagat tattgcagcc aatttcttta
      661 gcataatagc agaaaaatga caataaaata gcccctagat attttgaaag gaaagcatag
      721 gggctatata gtttaatcaa aataaagcaa atctttgctg gtgcttgtaa agaggtcgaa
      781 gccatccttg gtaacaacac cgcagtcttc aatacgaaca ccgactttac cagggatata
      841 gatacctggt tcaacagaga agcacatgcc ttcttcgatg accatgtcgt ttccttccat
      901 gatagatggg aattcatgga catccatacc gataccatgc ccgagacggt gattgaagta
      961 ctcaccataa ccagcttttt cgatgacctc acgggcagcg cggtccactt catgagcagt
     1021 cacacctggc ttgataaagt caagagcagc ttgttgggct tcaagagtca agttgtaaat
     1081 atctttcttg aattggtctg gtttgccgac agcgactgta cgagtcatat ctgacgcata
     1141 gccattgacc agaacaccca ggtcaaagag gagaagagca tcattttcaa ccttattagc
     1201 tgctggaatg ccgtgtggat tcgcagcatt atcaccagtc aagaccatgg tatcaaagct
     1261 catttcataa ccttcacgtt tcatggcaaa gtcgatttgt gcgatgatat ctgtctcagt
     1321 cttatcaaga gaaatattgt caaaaccaac atgaacagcc ttgtcagcat aaagacctgc
     1381 aaccatcatt ttttgcactt catcagctga tttgatgagg cgcatgcgtt ggatacgagg
     1441 agtgaggttg tcaaactcag cagtctcaaa aactgttttc aaaccatggt atttggtcaa
     1501 gatgagattg tcaaactcaa cagcgacacg tttgaagtca agttgtggaa gagcatgttt
     1561 gattttttgc catggatttt cagaatcgac atagcccact actgggaagg aaacggtgct
     1621 acttgcacgt tctacttcaa gagctgggac aaagaggaga ggttcctgat ccgctaggac
     1681 aaagaggaac atttggcgtt catggggatc actgtaaaaa ccagtgaggt aattgattgt
     1741 gacggggtca gatacgacag cgacgtctag tttttctgat tcaagatatg ttaggatttg
     1801 ttgtaattta gacatatgct accttctttc tacccctcta ttttggcaaa aaatgagaga
     1861 aaatgcaagg gagaagggta aaagaaaaga caaaaaactc cgacaagata acggagttct
     1921 ttgatatcat tttcctttgt ttaagaaaag tcagccttgc ttttgacgtc actgtactct
     1981 tcagcgattt cttggctgag gatatcctca taggcaggga gttggatatc ctgaccatat
     2041 ttcttcttga gggcagtagt aactaggcga taagccagat tggcattacg agagaggata
     2101 ggcccgtgga agtaggaacc aaagacattc ttataatgaa ccccttcacc gaccttttct
     2161 tcgttgtttc catttccata gacaacctgt cccagcggtt tttggtcatc agagaggaag
     2221 gtacggcctt ggtgattttc aaatccatag taggtttcat cgaaatcttc attgtgaatc
     2281 ttgatgtcac cgataaaacg gttattggtc tggttgagcg tgtagtgtcc catgacccct
     2341 agcccttcga tacgttttcc tgaagcttca acataatatt gacccaatag ttggaaacca
     2401 ccgcagatag ccagaactac accgtcgttt tggatgtagt tgtcaatgct ctctttttta
     2461 gcaggtaggt cgtctgcaat gatactttgt tcaaagtctt gaccaccacc gaaaaaggcg
     2521 atgtcgtagt gattttcatc aaagtcatca tggagagaaa cgatgtcaac ggtcacatgg
     2581 gctcccagtt tttcagccac atatttgagc atgaggatgt ttccattgtc cccgtaggta
     2641 ttcatgagat ttccgtagag gtgggcaatg ttgagctgat aggggtaatt gccatctttt
     2701 gaggaaagtg aagtataaac cattagttca tctcctttct aacaatctga cgactagcca
     2761 gcagttcacg aaattccagc atggcagtat aagttgccag aatataggca tgcttgcagt
     2821 cttgattctc aatggtcttg agaacttgct ccagattact cgtttcagtg attttctcag
     2881 ctggatagcc agtcactcgg aggcgacgag cgatttcaga atgacgaaca ccgccagcgt
     2941 tgatttcagg aatgtccatg tcagtgattt gctcaaagtc tgcatcccag atccagctag
     3001 tgtcaattcc atctgcatag ttggcattaa ggaggacaga taggctaaat ggataaggtg
     3061 ctagtttgat catttcgata gcttgggttg caccgactgg atttttaatc aagacaaggg
     3121 tacatttctt gtcaccgata tgaaaggttt cttggcgtcc aaagacagca cggctcttgt
     3181 caaatccctg tttgatgagt tgcgaatcgg cacctaggaa acgggcgatg gccacagcag
     3241 ctagggcgtt atagatatta tagagcccgc cgatttggat accgtattct tggccgtcta
     3301 tgacaaagcg agagcgattg ttggtcaact caaccagttt tgtcaaacga tagtcgagat
     3361 caggacgttt acatccacaa ccttcacaga tataggcacc caagtttgca taggtattat
     3421 gctcatattt gaggatgcct tggcagtcag gacagagaat cccttcggta ttgtagtgag
     3481 ccagttgggc tggtcctttt tccaagtcaa aaccaaaata ctctataggg tttggaatag
     3541 ttggcttgta gaaaagtgga ctgtctccgt taaggagaac agtagcagtt ggaacttttc
     3601 gaatggcatc caatatcatg ttataggtag tatagatttc accgaaacgg tccatctggt
     3661 cacggaagat attagtaatg acaaaaagac taggctggat atagtcacag atacgagata
     3721 gactggcttc gtcaatttcg aggacggcaa tatttttccc agtttttgaa gatttggctg
     3781 ttaggaaggt tgttgcaatc cctgtaatca tgttggcacc gcttgggttg gttagaactt
     3841 gaccataaac ctcttttaaa atgccgacag tgagggcagt tgtcagggtt tttccatttg
     3901 ttccagtgac aacgacaatc tcgtagttct tagctaggtt ttgtaaaata tctttatcaa
     3961 attgaagggc gactttacct gggagcgtac ttccacgtcc aagacggctt aaaacgaagt
     4021 gggaagaacg cccagcaaga aggcccaaag tagtttttaa gttcatgttt ctattatatc
     4081 acaaaagagg gaaaagacag atttggtgtt tcgtagaagt aaaaacagcc caaagagggc
     4141 tgtcagttaa gatgagattt caacttaaat cgcaaacaag tcattcaagt tctcagccaa
     4201 ggttgggtga gtgaagattt gttttgtgaa gtaagtgtaa ggaatcttgt tgtccatagc
     4261 aacagtaatg atgttgatga tttcttgaga accttctgag aagatgcttg caccaagaat
     4321 ttcttttgtt tcagtattaa caacagcttt gaaagctccg cgaaggtctc catttacgtg
     4381 accacgaggc atggctgcaa cagggatttc tttcactgcg tatggaagtt tcaaatcagc
     4441 tgcttggctt tcagtcaaac caacttgtga aagtgcaggt gtgatgaaca tagtatttgg
     4501 cacattgaga cggtcctcaa gtgtgtagct gccatctcca gcaaggtagc tgtaaacaac
     4561 acggaagtca tcaagtgaaa tgtaagtaaa ttgaagacca ccgttgacat ctccaactgc
     4621 aaagacacca ggaacgtttg tttgacagtg tttatctact ttaatagcgc cacgttcagt
     4681 tagttcaata tctgtatttt caagttgaag tggttctaca tttggtttgc gtccagttgc
     4741 gtagagaagg gcgtcgaaac ggtaagtttc gtcttcagtt acgacaagca cttggtcacc
     4801 gtcgttttta atttcagtag tatggatatt ttgaagcaat tcaataccgt cttcttccag
     4861 gtattgttta gcaagagctg cgatggaagg ttctgcacga ggtaggaatg tatccaaggc
     4921 atctaggact gtaaccttgc ttcctagttt attgtaaagg ccagcaaatt caagaccgat
     4981 atttccgcca ccaaggactc caagtttttc aggcaattta tccaagcttt ggatacctgt
     5041 tgagtcaaag acgtttttgc ttgtagcaag tccagggatt ggcaagacgt ttgaaacagc
     5101 accagtgttg atgacgatag tttcagcagt caattcttgt ttttcatcac cagcttggat
     5161 ttcgatgact ttatttgaaa ggaagtgagc ttcagcatca aagatattga cgccagtacc
     5221 agcaacagtc gtatagtttt taccgttgag gcgaccagtg atcgtgtttt tggtagcaat
     5281 gacttcttca aaagacaagt ctttctcagc agcaaccagc aaagttttag ttgggataca
     5341 accgatgttg atacaagttc caccgtacat agctttgcta cgttcaacga gggcaacttt
     5401 tttgccagct gaagccaatt taccagctag tgttttacca gctttaccaa atccgataac
     5461 gattaaatca taagttaaca tttagaattc ctcctattcg aattacattc tagcacaaga
     5521 aaaaaacttt tgcacaattc tgctacgaaa aagacagaaa aagatgaaaa cgctttccag
     5581 acaaaaaact agtaattttt tgacaagctt gataaactaa aatcctatct atttttatat
     5641 aataaagaat aaatgttcga aaataggagg ttttatgaaa tcgaatcgtt atattattgc
     5701 ctttgctggg gtcattttac acttaatgct gggttcgact tatgcctgga gtgtttatcg
     5761 taaccctatt attgaaaaaa cgggatggga tcaggcttct gttgccttcg cctttagtct
     5821 agcaatcttt tgtttgggct tatcggctgc atttatgggg cgtttggtag aaaaatttgg
     5881 tccgaaagtc atgggaagtc tatctgcttt tctatacgca ggtggaaata tcttaacagg
     5941 atttgcaata gaccgtcagg agctgtggtt gttgtatctc gcttatggca ttttaggtgg
     6001 gcttggtttg ggagcaggct atattacccc tgtgtcgacg attataaaat ggtttcctga
     6061 taaacgtggt ctcgcaacag gtttagcgat tatggggttt ggttttgctt ctttattgac
     6121 tagtcccata gcgcaacacc tcatcgcagg ggtagggctt gtagaaactt tttatatttt
     6181 aggagcaagt tactttatta tcatgctcct agcttcacaa ttcattaagc gtccaaatga
     6241 gcaagagctt gcaattttat cttcttcagg gaaagaaaaa acagcctctt tgacgcaagg
     6301 aatggctgca aatcaggctc taaaaagcaa tcggttttat atgctttgga ttattttctt
     6361 tatcaacata gcttgtggtt taggcttaat ttcagcggca tcgccaatgg cacaggagat
     6421 ggctggcttg tctacaagtc atgcagcagt aatggtgggt gttttgggga ttttcaatgg
     6481 atttggtcgc ttgctctggg cgagtttgtc tgactatatc ggtcgccctc taacctttag
     6541 tatattactg cttgttaatc ttttcttttc tctctcactt tggctcttta cagattccgt
     6601 tttatttgta gtcgctatgt ctattttgat gacttgctat ggagctggtt tttcattgat
     6661 cccagcttat ctcagtgata tttttggaac caaggaattg gccgctctgc atggatatat
     6721 tttaacagct tgggcaatgg ctggtttagc gggacctatt ttattagcag agacttataa
     6781 aatggctcat tcgtacacac aaaccttgtt cgtttttctc attttataca gtatcgcctt
     6841 ggctttgtct tattatctag gtcgttcaat caaaaaagaa agtcaaaaac cgcttacatg
     6901 attttctgta gaaatgggac tatcttttgc tctggtcttg tgttatacta gataggttgc
     6961 aaagaaaaca gtacttttct tttgtggaaa agaagcaaca cgattttata tctagttata
     7021 tgaaaatacg tcataaaaag aaaagtataa actaagccct atagggtggc ttcgcaccac
     7081 ctttagaaag aagaataacg tgaaatttaa tgaattaaac ttgtctgctg atttgctggc
     7141 agaaattgaa aaagctggtt ttgtagaagc tagtcctatc caagaacaaa ctattccttt
     7201 ggcccttgaa ggcaaggatg ttatcggtca agctcagact ggtacaggaa aaactgcagc
     7261 ctttggcttg cctacccttg aaaaaatccg tacagaagaa gcgactatcc aagccttagt
     7321 catcgctcca actcgtgaac tagctgtcca aagtcaagaa gaactcttcc gctttggtcg
     7381 tagtaaggga gtcaaagtcc gttcagtata tggcggatca agcattgaaa aacaaattaa
     7441 ggctcttaaa tctggtgccc atatcgtggt gggaactcca ggtcgcctct tggacttgat
     7501 taaacgcaag gccttgaaat tacaaaacat tgaaaccctt atccttgacg aagcggatga
     7561 aatgcttaac atgggcttcc ttgaagacat cgaagccatt atttcccgtg tacctgagaa
     7621 ccgtcaaact ttgcttttct cagcaactat gccagatgcc atcaaacgta tcggtgttca
     7681 gtttatgaaa gcccctgaac atgtcaagat tgcggctaag gaattgacaa cagaattggt
     7741 tgaccagtac tatatccgtg ttaaggaaca agaaaaattt gacaccatga ctcgtctcat
     7801 ggatgtggca caaccagaac tcgctattgt atttggtcgt accaaacgcc gtgtggatga
     7861 attgactcgt ggtttgaaaa ttcgtggctt ccgtgcagaa ggaattcatg gcgacctaga
     7921 ccaaaacaaa cgtcttcgtg tccttcgtga ctttaaaaat ggcaatcttg atgttttggt
     7981 tgcgacagac gttgcagcgc gtggtttgga tatttcaggt gtgacccatg tctacaacta
     8041 cgatattcca caagatcctg agagttatgt tcaccgtatc ggtcgtacag gtcgtgctgg
     8101 taagtcaggt caatctatta cttttgttgc tccaaacgaa atgggttacc ttcaaatcat
     8161 tgaaaacttg actaagaaac gcatgaaagg tctcaaacct gcaagtgtag aagaatcctt
     8221 ccaatcaaaa aaacaggtag ctctcaagaa aatcgaacgt gattttgcag atgaaaccat
     8281 tcgtgccaat tttgagaaat ttggtaagga tgctcgcaaa ttggctgctg agtttactcc
     8341 agaagaattg gcaatgtata tcttgagtct gacagtccaa gacccagata gccttccaga
     8401 agtggagatt gcacgtgaaa aaccactacc gtttaaacca tcaggtaatg gtttcggtgg
     8461 taaagctaag ggaggtcgtg gaggccgtcg tggggacgac cgtcgagagc gtgatcgccg
     8521 tggcaatggt cgccgtgatg agttcaaaaa aggaagtcgt ggcaacgatc gttttgataa
     8581 ggaaaaacgt taccgtaagg ataataaaaa accacgcaat actttaagcg aaaagcaaac
     8641 aggctttgtt attcgtaaca aaggtgataa ataagaaaaa gcggttcaaa atgaatcgct
     8701 tttttatata aggagaccga atgtgaaaaa gacaaataaa attattatat tatcttttat
     8761 aatattatga tacacaaaaa taggcaactt gtcaatagaa aaggaacaat ttaattaaaa
     8821 atattctggt ttttaattaa attgtaagtc aatgcaagtc aataaggaat tttcagataa
     8881 ttattgaaaa agagacgttt ttatgttata atgatagcga ttaaattcga ggtgaaaaat
     8941 ggcacattta ttagaaaaaa ctagaaaaat tacatcaatt ttgaaacgct cagaggagca
     9001 gttgcaggat gagcttcctt acaacgctat tacgcgtcag ttagcggata ttattcattg
     9061 caatgcctgc attatcaata gtaagggacg tctgcttggc tattttatgc gttataaaac
     9121 aaatacagat cgcgtagagc aattcttcca aactaagatt ttcccagatg actacgttca
     9181 aggggctaat atgatttacg aaacagaagc aaacttacct gttgagcatg atatgagtat
     9241 tttccctgtt gagagtagag atgattttcc agatggcttg acgactattg caccgattca
     9301 tgtatcgggg attcgccttg gttctttgat tatttggcgt aatgataaaa aattcgaaga
     9361 tgaggacttg gttcttgttg agattgccag taccgttgtt gggattcagc ttcttaactt
     9421 ccaacgtgaa gaagatgaga aaaatattcg tcgtcgtact gctgtcacca tggcggtcaa
     9481 taccctttct tactccgaac tccgtgctgt ttcagcaatt ttaggggaat taaatggaaa
     9541 tgaagggaag ttgactgcgt cagtgattgc agatcgtatc ggaatcactc gctctgtgat
     9601 tgtcaatgct cttcgtaaac ttgagtctgc ggggattatt gaaagtcgct cacttggaat
     9661 gaagggaacc tatcttaagg tcttgatttc agatattttt gaagaagtga agaaaagaga
     9721 ttactaatga caaaggcttt aatttcgatt gattatacag aagattttgt tgctgatagt
     9781 gggaaactaa cagcaggtgc tccagctcag gcgatttcgg atgccatcag caaggtaact
     9841 cgattagctt ttgaacgagg agattatatc ttctttacta ttgatgctca tgaagaaaac
     9901 gattgtttcc atccggaaag taagttattt cctcctcata atctgattgg gacgagtgga
     9961 cggaatttat atggagattt ggggaacttt tatcaagagc atggttcaga cagtcgtgtc
    10021 ttttggatgg ataaacgcca ttactcagct ttttcaggga ctgacctaga tattcgtttg
    10081 agagagcgta gagtgtctac cgttatctta acaggtgtct tgacggatat ctgtgtccta
    10141 catacagcta tagatgccta taatctagga tatgacatcg agattgttaa accagctgtt
    10201 gcttccatct ggcctgaaaa tcatcaattt gccctaggtc atttcaaaaa tacactcgga
    10261 gctaagttag tagatgaaaa tctaaatgaa ctttctgagt aacctggttg gtgaaaatga
    10321 aaaaatttga aaaaaagtgt tgacaagcat cccaaaagtt gatatactag taaagtaatc
    10381 gatgcgggga tggcggaatt ggcagacgcg caggactaag gatcctgtga ccgctttagg
    10441 tcgtgagggt tcaagtccct ctctccgcat aggaaaagag ttagctaaag ctagctcttt
    10501 tgtatttctc taaaaaatta gaatccagtc aaatgtagtg gagaatcagc gaaaatatgg
    10561 gaaaactgat ccttgtttct gcttttgtca tagaagaata ataaagatta tcttcttcaa
    10621 attctccgat attctctaaa gttttgtgca agttgcacag aactttttta tttttttggt
    10681 catcttgcca tagaaatata aagcgttttc atatataata taattatcaa aagacaaaag
    10741 gagttcacct catggtagaa ttgaatctta aaaatattta caaaaaatat ccaaacagcg
    10801 aacactattc agttgaagat ttcaacttga acatcaaaga taaagaattt atcgttttcg
    10861 taggaccttc aggatgtggt aaatcaacta cactccgtat gattgctggt cttgaagaca
    10921 ttacagaagg tactgcatct atcgatggcg tggttgtcaa cgacgtagct ccaaaagacc
    10981 gtgatatcgc catggtattc caaaactacg ctctttaccc acacatgact gtttatgaca
    11041 acatggcttt cggtttgaaa ttgcgtaaat acagcaaaga agacattaac aaacgtgttc
    11101 aagaagcagc tgaaatactt ggattgaaag aattcttgga acgtaaacca gctgaccttt
    11161 caggtggtca acgtcaacgt gttgccatgg ggcgtgcgat tgtccgtgat gcgaaagtat
    11221 tcttgatgga cgaacctttg tcaaacttgg atgccaaact tcgtgtatca atgcgtgctg
    11281 aaatcgctaa aattcaccgt cgtatcggag ctacaactat ctatgtaact cacgaccaaa
    11341 cagaagcgat gacacttgca gaccgtatcg ttattatgtc agctactaag aaccctgctg
    11401 gtacaggtac tatcggacgt gtagaacaaa tcggcactcc tcaagaagtt tacaaaaatc
    11461 cagttaacaa attcgttgca ggattcatcg gaagcccagc tatgaacttc atcaacgtga
    11521 aattggttgg tagcgaaatt gtttctgacg gtttccgttt gaaagtgcca gaaggagcat
    11581 tgaaagttct tcgtgaaaaa ggctacgaag gaaaagaatt gatctttggt atccgtccag
    11641 aagacgtgaa tgcagaacct gctttccttg aaacattccc agactgtgtt gtaaaagcga
    11701 ctatctctgt atcagaactg cttggttcag aatctcacct ttactgccaa gttggtaaag
    11761 acgagtttgt tgcaaaagtt gatgctcgtg actacttgca aacaggtgca acagttgagc
    11821 ttggatttga cttgaacaaa gcacacttct tcgatgtaga aactgaaaaa acaatctact
    11881 aaaataaata aaattcaaag cactacaaga aaagatattt ctttatcaat tgtagtggag
    11941 agatatcagt taatctaggg agagaaacaa aatgcttctc tcctttttgc tagggaagtc
    12001 atattatgca tctatattgt gatgctcttt aatactcttc gaaaatctct tcaaaccacg
    12061 tcaacgtcgc cttgccgtac gtatgattac tgacttcgtc agttttatct gcaacctcaa
    12121 agctgtactt tgagcagttt acggctagtt tcctagtttg ctctttgatt tccattgagt
    12181 attatttgtg ggtaccatct acaagtgaag ctatatgcgt aaactacgtg agcaattgaa
    12241 ttcgaactag agaggtaata ataaatttat gctatagtta tggtgacttg tatgcttttg
    12301 attctagttt atcaaataat agattagaat tgtcagataa tatcattttg tgttataatg
    12361 aagaaaaaac agaggtgttc aaatgtcaga agcaggtcat aagtttttag caaaattggg
    12421 gaaaaaacgc ttacgtccag gtggaaagcg tgccacagat tggttaattg cagaaggagg
    12481 attttcaaaa gaaaagagaa tactagaggt tgcgtgtaat aggggaacta cagcaattga
    12541 gttggcacag cgttttggtt gcaagataac tgctgttgat atggatgctc aagctttaga
    12601 agtggctaaa aaatctgctg gaacggcagg tgttgctcat ttaatcagtt ttgaaagagc
    12661 aaatgcaatg aaacttcctt atcaagatgc tagttttgat attgttataa atgaagctat
    12721 gctgactatg caagccgatc aagctaagaa aaaatgtgta atggaatatc taagggtatt
    12781 aaaacctgga ggtcttctct tgacacatga tgtacttctt aaggaagcta aagagtctat
    12841 cagacaggaa ttatcacaag caattcatgt aaatgtaggt cctttaactc aagatggttg
    12901 ggaacaggtg atgatagaat caggttattg tgatgtgaaa gcattgactg gtgaaatgac
    12961 attaatgaaa ttatcgggta tgatttatga cgaaggtttg ctaggaactt tgaaaatttg
    13021 tgtaaatgct tgtaaaaagg agaatagaaa gcagttttta actatgtata aaatgtttgc
    13081 taagaataaa cagaaattgg gctttattgc gatggctagt tataaatcgt caaaacgtta
    13141 gataattatt gaagttaact tttccttttt tctttcttaa aaaatatgct ataatagaga
    13201 gtaaaaaact ttgaaagaaa gaaaaagatg aatttaaaag attacattgc aacaattgaa
    13261 aattatccaa aggaaggcat taccttccgt gatattagtc ctttgatggc tgatggaaat
    13321 gcttatagct acgctgttcg tgaaatcgtt cagtatgcta ctgacaagaa agtcgacatg
    13381 atcgtgggac ctgaagctcg tggatttatc gtgggttgtc cagttgcctt tgagttggga
    13441 attggttttg cgcctgttcg taagccaggt aaattgccac gcgaagttat ttctgctgac
    13501 tatgaaaaag agtacggtgt cgataccttg actatgcacg cggatgccat taagccaggt
    13561 caacgtgttc ttattgtaga tgaccttttg gcgacaggtg gaactgttaa ggcaactatc
    13621 gagatgattg aaaaacttgg tggtgttatg gcaggttgtg ccttccttgt tgaattggat
    13681 gaattgaacg gccgtgaaaa aattggtgac tacgactaca aagttcttat gcattattaa
    13741 tgaaaacagt ccctagggct gttttctcta cactaggata taaaaataga ctataactag
    13801 ttagagaaaa actataattg aaaactatat cttcttacag tataataaaa ggactaagtg
    13861 tttgagattt gtcttcaaac atatgcaatt attcctgaaa gagtacagtt aggagagggt
    13921 tatgccgatt cgaattgata aaaaattgcc agctgttgag attttacgga cagagaatat
    13981 ctttgtcatg gatgatcaac gtgctgccca ccaagatatc cgtcctttga agattttaat
    14041 tttaaatctc atgccacaga aaatggtcac agagacccag ttgttgcgcc acttggctaa
    14101 tacaccccta caactggata ttgattttct ctatatggag agccaccgtt ctaaaacaac
    14161 tcgttcagag cacatggaga ccttctataa aacttttcct gaagtcaagg atgagtattt
    14221 tgatgggatg atcatcacgg gtgctccagt tgagcattta ccatttgagg aagtggacta
    14281 ttgggaggaa tttagacaga tgcttgagtg gtctaagact catgtctatt cgacccttca
    14341 tatctgttgg ggggctcagg ctgggcttta tctgcgctat ggtgtagaaa aataccagat
    14401 ggacagtaag ctatcaggta tttatcctca ggacacccta aaagagggtc accttctatt
    14461 tagaggcttt gatgatagct atgtatcccc tcattcacgg cacacggaga tttctaagga
    14521 agaggtctta aacaagacca atctcgagat tttatcagaa ggacctcagg ttggggtttc
    14581 tattttggcc agtcgtgatt tacgagaaat ttatagtttt ggtcatttgg agtatgaccg
    14641 tgataccttg gcaaaagagt attttcgaga tcgtgatgca ggtttcgacc cacatattcc
    14701 agaaaattac tttaaagatg atgatgttaa tcaggtacct tgtctttgtt ggtcttcatc
    14761 tgcagccctc tttttcagta attgggtaaa ccatgcggtc tatcaggaga cgccttttga
    14821 ttggagaaag atagaagatg atgcatctgc atatgggtat ttataagagg aattatgaca
    14881 tatttagacg cttttaaatc aggtatcttg gttttaccga gtgccctgct cttgcatttt
    14941 aaggaactct ttccttctag cgacgatttt ctggtttggc aatttttcta tttgcaaaat
    15001 acgacaggct tagaagaaat gtcgccaagc cagattgctg aaaggattgg caaggaaatt
    15061 tcggatgtca accagtccat ttctaatctg acggaaaggg gactgctcca gtatcgtact
    15121 atcgaattaa atggcgaaat tgaattgctc tttgatgcta gtttggcctt ggaacgtttg
    15181 gatgacctgt ttggagcagt tcattcaagt tcagaccagc tgacacctca aaaccagctc
    15241 aaggatttgg tggaaacctt ccagcaggag ttgggacgat tgttgacgcc ttttgagatt
    15301 gaggatttga ccaagacact aaaggaagat ggaaccagtg ctgacttgat taaggaggct
    15361 cttcgtgaag ctgttttgaa tggaaaacca aactggaagt acattcaggc gattttgaga
    15421 aactggcgcc atgaaggaat caagagtgtg gctcaaattg aggccaagag agcagaaaga
    15481 gaagcaagca atcctcagtt gacacaggta tctgcagatt tcataaatgc catggatctc
    15541 tggaaggatt aatccatgca agtaggcttg aaatccgagt aagatttgca agctgtgtat
    15601 aattgtgata gaataaatag aaaataaatt gaaaaaagag gtatgtgaaa tgtcacgtaa
    15661 accatttatc gctggtaact ggaaaatgaa caaaaatcca gaagaagcta aagcattcgt
    15721 tgaagcagtt gcatcaaaac ttccttcatc agatctggtt gaagcaggta tcgctgctcc
    15781 agctcttgat ttgacaactg ttcttgctgt tgcaaaaggc tcaaacctta aagttgctgc
    15841 tcaaaactgc tattttgaaa atgcaggtgc tttcactggt gaaactagcc cacaagtttt
    15901 gaaagaaatc ggtactgact acgttgttat cggtcactca gaacgccgtg actacttcca
    15961 tgaaactgac gaagatatca acaaaaaagc aaaagcaatc tttgcgaacg gtatgcttcc
    16021 aatcatctgt tgtggtgaat cacttgaaac ttacgaagct ggtaaagctg ctgaattcgt
    16081 aggtgctcaa gtatctgctg cattggctgg attgactgct gaacaagttg ctgcctcagt
    16141 tatcgcttat gagccaatct gggctatcgg tactggtaaa tcagcttcac aagacgatgc
    16201 acaaaaaatg tgtaaagttg ttcgtgacgt tgtagctgct gactttggtc aagaagtcgc
    16261 agacaaagtt cgtgttcaat acggtggttc tgttaaacct gaaaatgttg cttcatacat
    16321 ggcttgccca gacgttgacg gtgcccttgt aggtggtgcg tcacttgaag ctgaaagctt
    16381 cttggctttg cttgactttg taaaataatc agtaagtagc aaaagctagg tggaacagca
    16441 ttcagatgtc tgttacattt tttataggag aggaaagatt gaaaacaaaa attggattag
    16501 caagtatctg tttactaggc ttggcaacta gtcatgtcgc tgcaaatgaa actgaagtag
    16561 caaaaacttc gcaggataca acgacagctt caagtagttc agagcaaaat cagtcttcta
    16621 ataaaacgca aacgagcaca gaagtacaga ctaatgctgc tgcccactgg gatggggatt
    16681 attatgtaaa ggatgatggc tctaaagctc aaagtgaatg gatttttgac aactactata
    16741 aggcctggtt ttatattaat tcagatggtc gttactcgca gaatgaatgg catggaaatt
    16801 actacctgaa atcaggtgga tatatggccc aaaacgagtg gatctatgac agtaattaca
    16861 agagttggtt ttatctcaag tcagatgggg cttatgctca tcaagaatgg caattgattg
    16921 gaaataagtg gtactacttc aagaagtggg gttacatggc taaaagccaa tggcaaggaa
    16981 gttatttctt gaatggtcaa ggagctatga tgcaaaatga atggctctat gatccagcct
    17041 attctgctta tttttatcta aaatccgatg gaacttatgc taaccaagag tggcaaaaag
    17101 tgggcggcaa atggtactat ttcaagaagt ggggctatat ggctcggaat gagtggcaag
    17161 gcaactacta tttgactgga agtggtgcta tggcgactga cgaagtgatt atggatggtg
    17221 ctcgctatat ctttgcggcc tctggtgagc tcaaagaaaa gaaagatttg aatgtcggct
    17281 gggttcacag agatggtaaa cgctatttct ttaataatag agaagaacaa gtgggaaccg
    17341 aacatgctaa gaaagtcatt gatattagtg agcacaatgg tcgtatcaat gattggaaaa
    17401 aggttattga tgagaacgag gtggatggtg tcattgttcg tctaggttat agcggtaaag
    17461 aagacaagga attggcgcat aacattaagg agttaaaccg tctgggaatt ccttatggtg
    17521 tctatctcta tacctatgct gaaaatgaga ccgatgctga gaatgacgct aaacagacca
    17581 ttgaacttat aaagaaatac aatatgaacc tgtcttaccc tatctattat gatgttgaga
    17641 actgggaata tgtaaataag agcaagagag ctccaagtga tacagacact tgggttaaaa
    17701 tcatcaacaa gtacatggac acgatgaagc aggcgggtta tcaaaatgtg tatgtctata
    17761 gctatcgtag tttattacag acgcgtttaa aacacccaga tattttaaaa catgtaaact
    17821 gggtagcggc ctatacgaat gctttagaat gggaaaaccc ttattattca ggagaaaaag
    17881 gttggcaata tacctcttct gaatacatga aaggaatcca agggcgcgta gatgtcagcg
    17941 tttggtatta agcgatgatt tgaaagaggg atgtgatagt agcaccctct ttttctttgt
    18001 tttatgatag ttcatcctcg agtaaattca agatcttgct cggaaatgaa gcttatatag
    18061 tagattgaat atagacaaag accttgtgat tggtaaaaca ttttagaaat tcatttacct
    18121 ttcctaatcg acttggtttc atcttatttc aatctattat agtattgggg aatttcttca
    18181 aaccacatca gcttggtcag ttctacctgc gacctcaaaa cttgtgcttt ggtcaagctg
    18241 ggtttagttt cctagtttgc tgatggattt ccattgacta taagcatcca accctctttt
    18301 ttgtcttcta aagaattctt aaattatcag tctattgcaa cttttctcat ataagttctt
    18361 tgtcttgcta ttagttttcc ttagtagtat actaaggtag taatcattaa gaagtggtta
    18421 caaaaaataa tgaatgaggt aaagaaaatg gtagaattga aaaaagaagc agtaaaagac
    18481 gtaacatcat tgacaaaagc agcgccagta gcattggcaa aaacaaagga agtcttgaac
    18541 caagctgttg ctgatttgta tgtagctcac gttgctttgc accaagtgca ctggtatatg
    18601 catggtcgtg gtttccttgt atggcatcca aaaatggatg agtacatgga agctcttgac
    18661 ggtcaattgg atgaaatcag cgaacgcttg attacactcg gtggaagccc attctctaca
    18721 ttgacagagt tccttcaaaa tagtgaaatc gaagaagaag ctagtgaata ccgtaatgtt
    18781 gaagaaagct tggaacgtgt tcttgttatc taccgttact tgtcagaact tttccaaaaa
    18841 ggtttggatg tcactgatga agaaggtgac gatgtgacaa acggtatctt tgcaggcgct
    18901 aaaactgaaa cagataaaac aatttggatg cttgcagccg aacttggaca agcacctggt
    18961 ttgtaagaaa taaggaaaca tagataaatc tgtaggaaat ttcttacaga ttttttctat
    19021 tttgtaagct attccaaacc agtctttttt tgagtttttt gataaaatag tactatcagt
    19081 gaaaaggatg gaagtatgac taagaaaatc gtagctattt gggcccagga tgaagagggt
    19141 gtgattggta aggaaaatcg tctgccttgg catttgccag cagaattgca gcactttaaa
    19201 gaaacaactc tgaatcatgc tatcttgatg gggcgtgtga cctttgatgg gatggggcgt
    19261 cgcttgcttc caaaacggga aaccttgatt ttgacgcgta atccggaaga aaagatagat
    19321 ggggttgcta cttttcagga cgtccagtct gttcttgact ggtatcaggc tcaagaaaag
    19381 aatctctaca ttattggtgg gaagcaaatt tttcaggctt ttgaacctta ccttgatgaa
    19441 gtgattgtca ctcacattca tgctcgtgtg gaaggagata cctatttccc tgaagaactt
    19501 gacttgtctc tttttgagac agtttcaagc aaattttacg ccaaagatga gaagaatcct
    19561 tatgatttta ccatccaata ccgcaagaga aaggaactct aatggaacgg agtttatttg
    19621 gtctattcac ggcttttctg tgtttcatct gctttctggc aggagcacaa gcttttcgta
    19681 aaaagcgtta cgggctttct atcctccttt ggttaaatgc ctttaccaat ctggtcaata
    19741 gtattcatgc tttttacatg accttatttt agatagaatg acagtataga atggaaggaa
    19801 atcatgtcta caaatagaaa aaatgatatg atggtttatt gctcattttg tggcaaaaac
    19861 caagaagaag tacaaaaaat aattgctggc aacaatgctt ttatttgtaa tgaatgcgtg
    19921 gagttagctc aggaaatcat tcgagaagaa ttggttgagg aagtcttggc agacttgtct
    19981 gaggtgccaa aaccaattga actcctccat atcttgaacc actatgtaat tggtcaagat
    20041 cgtgccaagc gtgccttggc agtggcggtt tataaccact acaaacgcat caatttccac
    20101 gatacacgcg aagagtcaga agatgtagat ttgcagaagt caaacatttt gatgattggc
    20161 ccaactggtt cagggaaaac tttccttgcc cagaccttgg ctaagagctt gaatgtgcct
    20221 tttgctattg cggatgcgac agctctgacg gaggctggtt atgtgggtga ggatgtggaa
    20281 aatatcctcc tcaaactctt gcaggttgct gactttaaca tcgaacgtgc agagcgtggc
    20341 attatctatg tggatgaaat tgacaagatt gccaagaaga gtgagaatgt gtctatcaca
    20401 cgtgatgttt ctggtgaagg ggtgcaacaa gcccttctca agattatcga gggaactgtt
    20461 gctagcgtgc cgcctcaagg tggacgcaaa catccacaac aagagatgat tcaagtggat
    20521 acaaaaaata tcctcttcat cgtgggtggt gcttttgatg gtattgaaga aattgtcaaa
    20581 caacgtctgg gtgaaaaagt catcggattt ggtcaaaaca ataaggcgat tgatgaaaac
    20641 agctcatata tgcaagaaat catcgctgaa gacattcaaa aatttggtat tatccctgag
    20701 ttgattggac gcttgcctgt ttttgcggct cttgagcaat tgaccgttga tgacttggtt
    20761 cgcatcttga aagagccaag aaatgccttg gtgaaacaat accaaacctt gctttcttat
    20821 gatgatgttg agttggaatt tgacgacgaa gcccttcaag agattgctaa taaagcaatc
    20881 gaacggaaga caggggcgcg tggacttcgc tccatcatcg aagaaaccat gctagatgtt
    20941 atgtttgagg tgccgagtca ggaaaatgtg aaattggttc gcatcactaa agaaactgtc
    21001 gatggaacgg ataaaccgat cctagaaaca gcctagaggt gactatggaa cttaatatac
    21061 acaatgctga aatcttgctc agtgcagcta ataagtccca ctatccgcag gatgaactgc
    21121 cagagattgc cctagcaggg cgttcaaatg ttggtaaatc cagctttatc aacactatgt
    21181 tgaaccgtaa gaatctcgcc cgtacatcag gaaaacctgg taaaacccag ctcctgaact
    21241 tttttaacat tgatgacaag atgcgctttg tggatgtgcc tggttatggc tatgctcgtg
    21301 tttctaaaaa ggaacgtgaa aagtgggggt gcatgattga ggagtactta acgactcggg
    21361 aaaatctccg tgcggttgtc agtctagttg accttcgtca tgacccgtca gcagatgatg
    21421 tgcagatgta cgaatttctc aagtattatg agattccagt catcattgtg gcgaccaagg
    21481 cggacaagat tcctcgtggt aaatggaaca agcatgaatc agcaatcaaa aagaaattaa
    21541 actttgaccc aagtgacgat ttcatcctct tttcatctgt cagcaaggca gggatggatg
    21601 aggcttggga tgcaatctta gaaaaattgt gaggaaaaga aaatggcaaa aacaattcat
    21661 acagataagg ccccaaaggc tatcggaccc tatgttcaag gaaaaatcgt tggcaacctt
    21721 ttgtttgcta gcggtcaagt tcccctatcc cctgaaactg gggaaattgt aggagagaat
    21781 atccaagaac agacagagca agtcttgaaa aacatcggtg ctattttggc agaagcagga
    21841 acagactttg accatgttgt caaaacaact tgcttcttga gcgatatgaa cgactttgtt
    21901 ccttttaatg aggtttacca aacggccttc aaagaggaat tcccagctcg ttcagctgtg
    21961 gaggtagctc gtcttcctcg tgatgtaaaa gtcgaaattg aagtcatcgc agagattgga
    22021 taagctagtt gaagtttggt gttgccaaac ttcttttgat ataaggagaa aaagatgaca
    22081 aagaaacaac ttcacttggt gattgtgaca gggatgagtg gcgcagggaa aactgtagcc
    22141 attcagtcct tcgaggatct aggttatttc accattgata atatgccgcc agctctcttg
    22201 cctaagtttt tgcagctggt tgaaattaag gaagacaatc ctaagttggc cttggtagtg
    22261 gatatgcgta gccgttcttt cttttcagag attcaagctg ttttggatga gttggaaaat
    22321 caagatggtt tggatttcaa aatcctcttt ttggatgcgg ctgataagga attggtcgct
    22381 cgttacaagg aaaccagacg gagtcaccca ctagcagcag acggtcgtat tttagatgga
    22441 atcaagttgg aacgtgaact cttggcacct ttgaaaaata tgagccaaaa tgtggtggat
    22501 acgactgaac tcactccacg tgagctgcgc aaaacccttg cagagcagtt ttcagaccaa
    22561 gaacaagccc agtctttccg tatcgaagtc atgtctttcg gatttaagta tggaatcccg
    22621 attgatgcgg acttggtctt tgatgtccgt ttcttgccaa atccctatta tctaccagaa
    22681 ctgagaaacc aaacgggtgt ggatgaacct gtttatgatt atgtcatgaa ccatcctgag
    22741 tcagaagact tttatcaaca tttattggcc ttgattgagc cgattctgcc aagttaccaa
    22801 aaggaaggta agtccgtttt gaccattgcc atgggatgta cgggtggaca acaccgtagt
    22861 gtggcatttg ctaaacgctt ggcgcaggac ttatccaaga attggtctgt taatgaaggg
    22921 catcgcgaca aagaccgcag aaaggaaacg gtaaaccgtt catgagaaaa ccaaagataa
    22981 cggtgattgg tggagggact ggaattcccg tcattctaaa aagtctgcgg gaaaaagatg
    23041 tggaaatcgc agctatcgtg acggtggcag atgatggtgg ttcttcaggt gaactccgaa
    23101 aaaatatgca acagttgaca ccgccaggtg atcttcgtaa tgtccttgtg gccatgtcgg
    23161 atatgcctaa gttttatgag aaggtctttc agtatcggtt ctctgaggat gccggagcct
    23221 ttgctggcca tccattggga aatctcatca ttgctggctt gtcagaaatg cagggttcaa
    23281 cctataatgc catgcagtta ttgagcaaat ttttccatac aacagggaaa atttatcctt
    23341 ccagtgacca tcctttgacc cttcatgcag tctttcagga tgggacagaa gtggttggag
    23401 agagtcatat tgtagaccat cgaggcataa ttgacaatgt ctatgtgact aatgctctaa
    23461 acgatgatac gcctctggcc agccgtcgag tagtgcagac catccttgaa agtgacatga
    23521 ttgtcctagg gccaggttcc ctctttacct ctattttgcc caatatcgtg attaaggaaa
    23581 ttgggcgggc tcttttggaa accaaggcag aaattgccta tgtctgcaat attatgaccc
    23641 aacgtggaga gacggaacac tttacagata gcgaccacgt ggaagtcttg catcgtcacc
    23701 ttggtcgccc ttttatcgac actgtcttgg tgaatattga aaaagtgcct cagaaataca
    23761 tgaattccaa ccgttttgat gaatacttgg tgcaagtgga acacgatttt gtaggtcttt
    23821 gtaagcaagt ttcgcgcgtg atttcatcta acttccttcg tctggaaaat ggcggtgcct
    23881 tccacgatgg agatttgatt gtggacgagt tgatgcgcat tatacaggtg aaaaaatgag
    23941 tttcacagta gcagtaaaag aagaaatcct gggccaacac catctgagcc ggcatgaatt
    24001 atctgccatt atcaaaatgt ctggtagcat cggtctctcg acttcgggct tgactttgtc
    24061 agttgtgaca gaaaatgcca aactggcccg tcacctctat gagtcctttc tccatttcta
    24121 tgaaatcaaa tctgaaattc gtcaccacca acggagcaat cttcgcaaga atcgcgtcta
    24181 taccgttttt acagatgaaa aggtgcagga tttgttaagt gatttgcact tggcagactc
    24241 tttctttggc ttggaaacag gtattgatga ggcgatttta tcggatgagg aagcaggtcg
    24301 tgcctatctc tgtggcgctt tcttggcaaa tgggagcatt cgtgaccctg agtcaggcaa
    24361 gtaccagttg gaaatcagtt ctgtttatct ggaccacgcg caaggaattg cctcccttct
    24421 ccaacagttt ttactggatg ccaaggtgct tgagcgcaag aagggggctg tgacctatct
    24481 ccagcgagca gaagacatta tggacttctt gatagtaatc ggagccatgc aggcacgtga
    24541 tgattttgag cgggttaaga ttttgcgaga aactcgtaac gacctcaatc gggctaataa
    24601 tgccgagaca gctaatatcg ctcggacagt ttctgccagc atgaagacca ttaacaatat
    24661 cagtaaaatc aaagatatca tgggcttaga aaatttgcca gtagatttgc aggaagtggc
    24721 gcaactgagg attcagcacc cagactactc tatccagcag ttagcagata gcctcagcac
    24781 ccctctgacc aaaagtggtg tcaatcacag actcagaaaa atcaataaga tagccgatga
    24841 attataaaac cacgaatcct atgtgactcg tggttctttt ttataaacta gtagagtgtt
    24901 ttggttgtac tttttgttca gggtcaatgt agttgatagc gttgttgaca gcagttggag
    24961 cttctccgag gcctgtcgca atcagatcaa tttttccgtc atagtagcag cagtcaccga
    25021 tagcatagat acctgcttgg ctggattcct gtttgctgtt gacgataatc ttgtgacggt
    25081 tgaggtcgag cccccagttt ttaaggttac cgacagaaga tttgaaacca tagttgacaa
    25141 agaggtggtc taggtcaatg gtttcagttt catcagattt gacttttgtg atttcaagtt
    25201 tatcaagtgt ttttccattt ccaaggagtt ggctaggggc gaatggtgtc ttgatggtta
    25261 cagatgattc ttgcaaggct tgaacactgt gttccaaggc acggaaatta tctctgcggt
    25321 gaacaagggt agttggtgcg attttttcaa aagccaaagc ccaatccaca gccgagtctc
    25381 ccccaccaag aatcgtcact ttcttaccag cgtattgctg aatgttagaa acgtggtagt
    25441 ggatattttc atagccctca accccttcaa gttccagcgg acgtggtttg aaggcaccgc
    25501 cacccatagc gatgatgact gttttagtca agtgacttcc tttagaagtt gtgatggcaa
    25561 attcttcttc ttgtttgtca atctcaagaa ccgtttcatt gagatgaata ggggtatcaa
    25621 atccatttag ctgttcaatc aagcggttag tcaactcttc tccagtcagg tttgggaagc
    25681 ctggtacgtc taggatttcc ttttcagggt agagaatagc aggttgtcca cctagctggg
    25741 gaagagagtc gatgatttga accttggctt ggcgtaggtg ggcataaaag gctgcaaaaa
    25801 gcccgacagg accaccaccc acaatggtaa tatcatagag ttgagacata gtttctcctt
    25861 tgttttttct agtcagttta ttttatcata tttttgttca atgtaaactg gactaggata
    25921 gggaaaaaaa tgccagaaaa tggtataata ggacgaaagt gttttgatag ggaggatatc
    25981 agggatgaat tttcaacaat tatccaatct gcaatattgg accagtttgt ttgcaagtcc
    26041 atggacgata gctatcaatc tgattgatat tttgattgtt gcttatattt tataccattt
    26101 tacaaaagct attgcaggaa ccaagattat gattttggta cgtggagttt tggtgtttat
    26161 tttagctcaa atccttgcaa atatgattgg tttgactacg atttcttggt taatcaatca
    26221 aattattact tatggggtta ttgcggcggt tgttatcttc tctccagaga ttcggactgg
    26281 tttggaacgt ttgggaagag cgacagattt cttttccaat gcccctatta gtgctgagga
    26341 acagatgatt cgtgcctttg ttaagtctgt tgaatacatg agtcctcgta aaatcggggc
    26401 cttggttgct attcagcgtg tacgtacctt gcaggagtat atttcgacag gaattccctt
    26461 ggatgctaag atttctgcag aacttctcat taacattttt attcccaaca ctcccctaca
    26521 tgatggtgcg gtgattatca aagaagaacg tatcgctgtg acgtctgcct atctgccctt
    26581 gacaaaaaac acagggattt ccaaggaatt tgggaccaga caccgggcgg ctatcggttt
    26641 atcagaagtc tcagatgcct tgacttttgt tgtatcagag gaaacgggag gaatttcgat
    26701 aacctataat ggaaggttta agcacaacct aacacttgat gaatttgaaa cagaattacg
    26761 tgaaatctta cttccaaaag aggaagtggg tcttagtttt aaagaacgat tgctaggagg
    26821 atggaaacat gaaaaaaaat agtttatata tcatatcctc actctttttt gcttgtgtct
    26881 tatttgtcta tgctacggcg acgaattttc aaaacagtac cagtgctagg caggtaaaaa
    26941 cggaaaccta tactaataca gtaacaaatg tccctattga catacgctat aatagtgata
    27001 agtattttat tagcggtttt gcttcagaag tatcagtggt cttgactggt gcaaatcgcc
    27061 tatcgctagc tagtgaaatg caagaaagta cacgtaaatt caaggttact gctgacctaa
    27121 cagatgccgg tgttggaacg attgaagttc ctttgagcat tgaagattta cccaatgggc
    27181 tgaccgctgt ggcgactccg caaaaaatta cagtcaagat tggtaagaag gctcagaagg
    27241 ataaggtaaa gattgtacca gagattgacc ctagtcaaat tgatagtcgg gtacaaattg
    27301 aaaatgtcat ggtgtcagat aaagaagtgt ctattacgag tgaccaagag acattggata
    27361 gaattgataa gattatcgct gtcttgccaa ctagcgaacg cataacaggt aattacagtg
    27421 gttcagtacc tttgcaggca atcgaccgca atggtgttgt cttaccggca gttatcactc
    27481 cgtttgatac aataatgaag gtgactacaa aaccagtagc accaagttca agcacatcaa
    27541 attcaagtac aagcagttca tcggagacat cttcgtcaac gaaagcaact agttcaaaaa
    27601 cgaattaaaa atagaaaagg attttataac aatgggtaaa tattttggga ctgatggagt
    27661 ccgtggagaa gctaacctag aactaacacc agaattagcc tttaaactag gacgttttgg
    27721 aggctatgtt cttagtcaac atgaaacgga agcgccgaaa gtctttgtag gacgtgacac
    27781 acgtatttca ggggaaatgc tggaatcggc cttggtggca ggtctccttt cagtagggat
    27841 tcacgtatac aaacttggtg tccttgcaac accagcagta gcttacttgg ttgaaactga
    27901 aggagcaagt gccggtgtca tgatttctgc tagccacaac ccagcccttg ataacggaat
    27961 caagttcttt ggcggtgatg gcttcaaact agatgatgaa aaagaagcag aaattgaagc
    28021 cttgctagat gctgaggaag acactcttcc tcgtccaagt gcagaaggct taggaatctt
    28081 ggtagattat ccagaaggct tgcgtaagta tgaaggatac cttgtttcaa ctggaactcc
    28141 tcttgatgga atgaaggttg ccttggatac agctaatgga gcagcttcta ccagtgcccg
    28201 tcaaatcttt gcagaccttg gtgcccaatt gacggttatc ggggaaacac cagacggtct
    28261 taacatcaac cttaatgttg gttcaacaca tccagaagcc cttcaagaag tggtcaaaga
    28321 aagtgggtca gctattggtt tggcctttga tggagacagt gaccgcttga ttgctgttga
    28381 tgagaatggt gacatcgtcg atggtgacaa gattatgtac atcatcggaa aatacctttc
    28441 tgaaaaagga caattggctc aaaatacaat tgtgacaact gttatgtcta accttggttt
    28501 ccacaaggcc ttgaatcgcg aaggtattaa caaggcagtt actgcagttg gtgaccgcta
    28561 cgttgttgaa gaaatgagaa aatcaggcta caaccttggt ggtgaacagt ctggtcacgt
    28621 tatcttgatg gattacaata ccacaggtga tggtcaatta tcagcagttc aattgactaa
    28681 aatcatgaag gaaactggta agagcttatc agagttggcg gcagaagtaa cgatttatcc
    28741 acaaaaatta gttaatatcc gagtggaaaa cgtcatgaag gaaaaggcca tggaagtgcc
    28801 agctatcaag gccatcatcg agaagatgga agaagaaatg gcggggaacg gccgtatcct
    28861 tgttcgtcca agtggaacag agcccctctt gcgtgttatg gcagaagcgc ctacaacaga
    28921 agaagtagac tactatgttg ataccatcac agatgtagtt cgtgctgaaa ttgggattga
    28981 ttaaatccta gaaaactaga tgaaaataaa aaattatggt acaatagctt ataatattgt
    29041 agccgatgga gaaagacatg aatcttaaac gtgaacaaga atttgttagt cagtatcatt
    29101 ttgatgcgcg taattttgaa tgggaaaatg aaaatggagc tcctgaaacc aaggtggatg
    29161 tgaatttcca attgctccaa catgaccaag aaaatcaagt gacttccttg attgtcatct
    29221 tgagctttat gattgtattt gataagtttg tcattagtgg aacaatctca caagtaaacc
    29281 atatcgatgg ccgtattgtc aatgaaccaa atgaattgaa ccaagaagag gtggaaacct
    29341 tggctcgtcc atgtttgaac atgctcaatc gtttgactta cgaagtgact gaaattgcat
    29401 tagacttacc aggaattaat ttggagttct aatatgaaat tagctgtttt cacagattcc
    29461 tctgcttatc tcagtgcaga gaccttgcaa agagaagact tgtttgtctt ggatattcct
    29521 gtcaatattg atggtgagga atatgtcgaa ggcatcaatc tgtctgctga ggaattttac
    29581 caaaaaatgg ctcaggcttc tgaattgcct aagaccagtc aaccaagtat tgccaagtta
    29641 gatgaaatct taacttcgct caaagaacaa ggctatacac atgccttggg gcttttccta
    29701 tcttctggaa tttcaggttt ttaccaaaat atccagtata tggtcgatga ctatgagggc
    29761 ttaaccattg ctttcccaga cactttgatt acaagtgctc ccctaggcat catggttgaa
    29821 agcgtcttta attggcgtga tcagggcgat gattttgcca gcattcagga taagctagcc
    29881 attcaaatca gccgtacgtc agcctttatc atggtagatg acttggatca tttggtaaaa
    29941 ggtggacgtc tttcaaatgg ggctgccatt ttgggcaatt tgcttagcat taagccaatc
    30001 ctttatttta acgatcaagg tgtgattgaa gtttacgaaa aagttcgtac tgaaaagaag
    30061 gccaccaagc gcttaattga aattatcaag gaaacaacgg cttcaggtca ataccgggtc
    30121 attgtcattc atgggaatgc tcctgaaaag gctgaagaat tgcgtcagca tttgcttgat
    30181 tttggcttgg gttcggatgt ttcacttgct acatttggta gtgtcattgg aacgcaccta
    30241 ggagcaggaa gtattgctct gggttatatt ccagtgattt agttggcgct tctaggctag
    30301 ttaagatgcc ttgtatctta gcaattgaac acggactacc tgcttcttga aaaaagatgc
    30361 ctgtcttgcc tttcgtggaa agtcagtgcc attccctatt tttcaggggc agctaaggtc
    30421 ctttgtatct tgataattga acacgcctgg aaccctgtgt gaaaaagata gttcttccaa
    30481 gaagtagaca cttcttgatt ggaattccta ttttcacttt gtgttcttac ggactttgta
    30541 tcttagacag gagtagagat gagtattcga gtaattattg ccggttttaa gggaaagatg
    30601 ggccaggctg cttgtcagat ggtattgact gatccagact tggacttggt agcagttttg
    30661 gatccttttg agtctgagtc agaatggcag ggtattcctg ttttcaagga taaggctgat
    30721 ttagcttgtt ttgaagcgga tgtctgggta gattttacta ctccagctgt tgcctacgaa
    30781 aatacacgtt ttgctcttga aaatggcttt gctccagtag ttggaacgac tggtttcacg
    30841 agtgaagaaa ttgcagagct aaaagaattt tctcgtaccc aagacttggg tggcctgatt
    30901 gcccctaact ttgccttggg tgctgtctta ctcatgcaat ttgcgacgca ggctgccaaa
    30961 tatttcccaa atgtggagat tattgagctc catcatgaca agaaaaagga tgctccgagt
    31021 ggaacagcca ttaaaacagc tgagttgatg gcagagattc gagagtcaat tcagcaaggt
    31081 gcagcagatg aggaagagct gattgctggt gctcgtggtg ctgactttga tggtatgcgc
    31141 atccactcag ttcgtttgcc aggtttggta gcccatcagg aagtcatctt tggcaatcaa
    31201 ggagaaggat tgaccctccg tcatgactcc tatgatcgca tctccttcat gacaggagtc
    31261 aatttgggaa ttaaagaagt tgtcaagcgt catgagcttg tctatggatt agaacactta
    31321 ttatgagatt aatgcaaatg ccttctgaat ttcagaaggc tttaccagta ttagaaaaaa
    31381 ttaaagaagc aggctttgag gcttattttg ttgggggctc tgttcgagat gcccttctcc
    31441 atagccctat ccacgatgtg gatattgcga cgtcttctta tccagaagag accaagcaga
    31501 tttttccgcg aacagccgat atcggaatcg agcatggaac cgtcttggtc ttagatgggg
    31561 atgaggagta tgaggtaaca acctttcgga cagaggatgt ctatgtggac tatcgcagac
    31621 ccagtgcggt ttcctttgtg cgctcgctag aagaagacct caaacgccgt gatttcacag
    31681 tcaacgcctt tgccttggat gagacaggag aaatcgttga cttgttccat ggtttagaag
    31741 atttggaaaa gcaagtcttg cgagcagttg gagtggctag tgagcgtttc aacgaagatg
    31801 ctttacggat tatgcgtggt ttccgttttc aggctagtct tggttttgca cttgaaccag
    31861 aaacatttaa agctatgaag accttgacgc cgcttttgga gaacatttct gtagagcgta
    31921 ccttcgtcga gtttgataaa ctcttgctgg ctccattttg gagaaggggg ttggcttcca
    31981 tgattgagag tcaagcttat gactatctcc ctgatatggc atctagccag gacaagctca
    32041 acagactgtt tgatttggag actgatttta cttttgaatc ctctgaacaa gcctgggcgg
    32101 ctttactatg ggctttggag attgaaaatg cgcagtcatt tttgaaatct tggaagacct
    32161 cacgccagtt tgccaagcaa gttcaggatt tgctgattat tttggctctg cgtgaaaatg
    32221 gagaattgag caagcgagat tgttatcgct ttgacataga tttgctttta caggctgaaa
    32281 atcttcgtca agctcaagga aaagaagtca acccacaagc catcacagaa aaataccaaa
    32341 gcttgactat ccatgacaag aaagagattc agattaatgg cggtattttg atcaaggaat
    32401 atggctatca gccaggccca gacttgggag agattttaac agagattgag tttgccattg
    32461 tcgatggaga attggagaat aatcgtgaag ccatccatgc ttacctgagg gagaaaaaat
    32521 gagtgatttt atcgttgaaa aactaagtaa atctgttggt gacaagaccg tttttaggga
    32581 tatttccttt attatccatg acttagacag aattggttta atcggtgtca atgggactgg
    32641 caagaccacc cttttggacg tcctttctgg tgtttctggc tttgatgggg atgtcagtcc
    32701 tttttcagct aaaaatgatt accagattgg ttacttgact caggatcctg attttgatga
    32761 tagaaagaca gttttggata cgattctatc tagtgaactc aaggaaatcc agctcattcg
    32821 tgagtatgaa ttgattatgc tcgactatag cgaggacaag caggcgcgtt tggaacgtgt
    32881 catggcagag atggactctc tccaagcttg ggaaatcgag agtcaggtca agaccgttct
    32941 tagcaaattg ggcattcaag acttatctac tcctgttggg gaattgtcag gtggtctgag
    33001 aagacgggta cagttggcac aagtcttact tggcaaccac gacctcttgc ttttggatga
    33061 gccgaccaac catctggata ttgcgattat tgagtggctg accctctttt tgaaaaattc
    33121 taagaagacc gtccttttta tcactcacga tcgttatttc ttagacgctt tgtcaacacg
    33181 gattttcgag ttggagcgag caggcttgac agaatatcag ggcaattacc aggactatgt
    33241 tcgccttaag gcagaacagg acgagcgcga tgcagctcta cttcataaaa aagaacaact
    33301 ctacaaacaa gaactggtct ggatgcgcag acaaccgcag gcacgtgcga ccaagcaaca
    33361 agctcgtatc aatcgtttcc atgatctgaa aaaggaagtt tcaggcagta gtgctgagac
    33421 agacttgact atgaactttg aaaccagtcg gattgggaag aaagtcatcg agtttcaggc
    33481 tgtttccttt gcctatgaaa ataagcccat tttgcaagat tttaatctct tagttcaggc
    33541 taaagaccgt attggaattg ttggggacaa tggtgttgga aaatcaaccc tacttaacct
    33601 gattgcagga agtcttgagc cgacagcagg acaagttgtg attggggaaa ctgttcgcat
    33661 cgcctatttc tctcaacaaa ttgagggttt ggatgaaagc aagcgtgtga tcaattacct
    33721 gcaggaagtg gcagaggagg tcaagaccag tggtggttct acgacttcca tcgctgagtt
    33781 gctggagcaa ttcctcttcc cacgttcgac gcatgggacc ttgattgaga aattgtcagg
    33841 gggtgagaaa aaacgtcttt atctcctcaa actgcttttg gaaaaaccaa atgttcttct
    33901 tttagacgag ccaaccaatg acctagatat tgcaactttg acagtcttag agaatttctt
    33961 gcaaggtttt gcaggtcccg ttttaacagt cagtcacgac cgctatttct tggataaggt
    34021 agcgaccaag attctcgctt ttgaggatgg caagattcgt cctttctttg gtcattacac
    34081 cgactatctt gatgaaaaag cttttgaaac agatatggcc aatcaagtgc aaaaggccga
    34141 aaaggaaaaa gtggtcaagg ttcgagaaga caagaaacgc atgacctacc aagaaaagca
    34201 ggagtgggca agtattgaag gtgatattga aaccttggaa aaacgtatcg ctgctattga
    34261 agaggaaatg caggctaacg gctctgactt tggtaagctg gctactctcc aaaaagaatt
    34321 ggatgagaaa aatgaagcac tccttgaaaa atacgaacgc tatgagtatc tcagtgaatt
    34381 tgatagttaa tactattcga aaatcaaagt gtaaactatg tcggcttcac attgccgact
    34441 caagtacagc ctgtggccgg cttcctagtt tgcactttga tttttattga gtataaaaga
    34501 atagaaaaat cccatctcat gaacggattt ctctattctt ttttggtttc ttgatgaaag
    34561 atattttgcc aggtttcgtg gcgacgccag atactggtat ggatgatacc atctaactca
    34621 tagcagatga gtttggtttt ttgactgatg gaagtaattt gaatgtcctt gatagcagaa
    34681 tttaactctt ttttggtctt ataagcctct ttatccatct gctctccatc ttggcgaata
    34741 tagacaaaat catcagtcaa gagttcttct agttggtttc cttggtcaat caattgttca
    34801 cgcataagca attttttata gacattgtct aaaatttcat cctcagggat aggtgctggt
    34861 tcgatatgga catcggtatc aaagacgcca aaacgattct ccagcataga ctcgacctga
    34921 tccgcgattt catggctttc aaaaacagac aagtcaggat tcatctctag tgtaatatcc
    34981 aggtagatgt tgctaccgta ggtgcgacct ctttgcgatt tgaccttgct gattttggga
    35041 atttccatga tagccttttg gtagtcctcg agcaggcggt cgtcaaagcc atctgaaaga
    35101 ctaaaggaag actcgatgaa gatatcatag gcagtcttca agataaagaa agtgatgatg
    35161 atagcaacca gtttatccac aatcggataa ttgaaactac tagctaggat ggcaatggcg
    35221 gttccaagtg aggtaacagc gtcagaaaga ttgtccttag cagctgcttt cagcgccttg
    35281 gagttggatt tcttactgag gcgagtattg tagagataga ccacaaacat aatcgctgca
    35341 gaaatgattc ctagagttgc accaagagga tcaatgaccg tttcttcccg actgagaatc
    35401 ttttgaatgg tatctcttag aacatcgaaa ccgacataga acatgatgat agaagtgatc
    35461 aagcttgcca aatcttcaat cttccaatga ccaaaacggt ggtcacggtc tgcaggctgg
    35521 cgcgccatcc gaatcccgat taagagggcc acatttccaa tgatgtccga tacgttatta
    35581 aaaccatcgg ccaccaaact ggatgaatga aggagatgac cagttgctaa tttggctgca
    35641 gacaagatca aataggtcga aatactgata atggctccac gctcagctaa cttgagattt
    35701 gagatagatt gcttcattca acttcctttc tagtcacata gcattctacc attatactgg
    35761 ttttcaagga aaaattcaaa taaggcagtt tttactcttt gaaaactccc caaaatttgg
    35821 tataatagta ctactcaagg gagtagctgg cagaaacctg tgatagtgtc gtcattccga
    35881 attttatgct gaaaagtatg ctttccggcc ctatcttaaa cagcgagact tgttatgatt
    35941 aacaggtctc tttttgtttg tcgtgaaaag atatgacaga aagagagtct gttttgcaca
    36001 caatgtcaag gaggagacac atgtcaaaag aacaaaaacg ccaagcgttt tatactcaga
    36061 gccctgaaga ggtcttgcag gctgtggatg cgaccgagca aggtttgtcg tcaagtgagg
    36121 cggaaaagcg ccttgtcgaa tttgggcaca atgaactcga agaaggcgag aaacgatcaa
    36181 tcttggtcaa attcatcgag caatttaagg atttgatgat tatcatccta gttgcggcag
    36241 caatcttgtc agtcgtgact tctggtgggg aagatatcgc agatgccatt atcatcctag
    36301 ctgtggttat catcaacgct gcctttggtg tttaccaaga aggaaaagct gaagaagcta
    36361 ttgaagccct caaatccatg tctagtccag ttgcccgcgt tcttcgtgat ggacacatgg
    36421 cagagattga ctctaaagaa ttggtaccag gcgatatcgt tgcccttgaa gcaggtgacg
    36481 tggtaccagc ggacctacgt ttgatagaag ccaactctct taaaattgaa gaagcagcct
    36541 tgacaggtga atctgtacca gtcgaaaaag acttgtcagt cgagcttgcg acagatgctg
    36601 gtattggtga ccgtgtcaac atggccttcc aaaactcaaa cgtaacctat ggtcgtggga
    36661 tgggtgttgt tgtcaataca ggtatgtaca ctgaagttgg tcatattgct ggtatgcttc
    36721 aagatgcgga tgagactgat acaccactca aacaaaactt gaacaacctt tctaaggtct
    36781 tgacctatgc tatcttggcc attgcccttg ttacttttgt agtgggtgtc ttcattcaag
    36841 ggaaaaatcc acttggtgag ttgttgactt ctgttgccct tgccgttgca gccattccag
    36901 aaggacttcc tgctatcgtt accatcgttc tttctcttgg tactcaagtt ttggccaaac
    36961 gacattccat cgttcgtaag ttgccagcag ttgaaacact tggttcaact gaaatcatcg
    37021 cttctgataa gactggtacg ctgactatga acaagatgac agtcgaaaaa gtcttctacg
    37081 atgcggttct acatgactca gctgatgata ttgaactagg tcttgaaatg ccactacttc
    37141 gttcagttgt cttggccaat gatacgaaaa tcgatgtgga aggtaacttg attggtgacc
    37201 caaccgaaac agcctttatc caatatgcct tggacaaggg ctatgatgtc aaaggtttct
    37261 tagagaaata tcctcgtgta gctgaattgc catttgactc tgaccgtaag ctcatgtcaa
    37321 cagttcaccc attgccagat ggtcgtttcc ttgtagcagt caagggtgcg ccagaccaac
    37381 tcttaaaacg ttgtcttctt cgtgataagg ctggggatat tgctccgatt gatgagaagg
    37441 ttacaaatct cattcataca aacaattctg aaatggctca tcaagccttg cgtgtccttg
    37501 caggtgctta taagattatc gatagtattc cagaaaatct cacttctgaa gagcttgaaa
    37561 atgatttaat ttttactggt ttgattggga tgattgaccc tgaacgtcct gaagccgctg
    37621 aggctgttcg tgtggctaag gaagcgggaa tccgtccaat tatgattaca ggtgaccacc
    37681 aagacacagc ggaagccatt gccaaacgtt tgggaatcat tgacgcaaac gatacagaag
    37741 gtcacgtttt aactggtgct gaactcaatg aactgtcaga tgaagaattt gaaaaagtcg
    37801 ttggtcaata ctctgtttat gcccgtgtgt ctccagaaca caaggttcgt atcgtcaagg
    37861 cttggcaaaa acaaggtaaa gtcgttgcca tgacaggtga cggtgtcaat gacgcgccag
    37921 ctctgaaaac agccgatatc ggtatcggta tgggaatcac tggtacagag gtttctaagg
    37981 gggcttctga tatgattctt gcagatgata actttgcgac tattatcgtc gcagtggaag
    38041 aaggacgtaa ggtcttctca aacattcaaa agactattca gtacctactt tctgctaata
    38101 ctgctgaagt attaaccatc ttcctatcaa ccttgtttgg ttgggatgtc ttgcagccgg
    38161 ttcatctttt gtggatcaac ttggtaacgg ataccttccc agctatcgct cttggtgttg
    38221 aacctgcgga gcctggtgtc atgaatcata aaccacgtgg acgcaaggca agcttcttct
    38281 caggtggtgt tttgagttct atcatttatc aaggtgtact ccaagcagct cttgttatga
    38341 gtgtttatgg ccttgcgatt gcttacccag ttcatgtggg tgacaatcat gctattcatg
    38401 cagatgccct aacgatggcc tttgcaaccc ttggtttgat tcagctcttc catgcctaca
    38461 atgtcaagtc tgtctaccaa tccatcttga cagttggccc attcaagtct aagaccttta
    38521 actggtccat cttggtatct tttatccttc tcatggcaac aatcgttgta gaaccgcttg
    38581 aaggaatctt ccacgtaacc aaactagact tgtcacaatg gggaattgtt atggctggaa
    38641 gcttctcaat gattatcatc gtcgaaatcg ttaagtttat tcaacgcaaa ctcggttttg
    38701 acaagaatgc gatttaaggg aggtttcgga tgtcagcata tcaattaccg accgtatggc
    38761 aggatgaagc tagtaatcaa ggagctttta cggggctaaa cagaccaaca gcaggtgccc
    38821 gtttcgaaca aaacttgcca aaaggagaac aagcttttca gctttattca ctgggaacac
    38881 caaatggtgt gaaggttact atcttattgg aagaattact agaagctggt tttaaggaag
    38941 cggcttacga cttgtataag attgctatca tggatgggga tcaattcggc tcagactttg
    39001 tgaagctcaa tccaaattcc aagattccag ccttattgga ccagtcaggt actgaaaacg
    39061 taagagtctt tgagtctgct catattcttc tttaccttgc tgagaaattt ggagcctttt
    39121 taccaagtaa tcctgtggaa aaggtagaag ttttgaattg gctattctgg caagcaggtg
    39181 cagcaccttt tctaggtggg ggatttggac atttcttcaa ttatgctcct gaaaaattgg
    39241 aatatcctat taaccgtttt acgatggaag tgaaacgcca gttggattta ttggataagg
    39301 aattggctca gaaaccttat attgcaggca atgactatac gattgcagat attgctatct
    39361 ggtcttggta tggacagtta gttcaaggaa atctttacca aggttctgca aaattcttgg
    39421 atgcctcaag ttatcaaaat ctagtaaaat gggcagaaaa aattgccaat cgtccagctg
    39481 ttaagcgtgg attggaagta acttatacag aaattaaata gcaaaaaagt catcttcgga
    39541 tggctttttg ctacagttga gggaaagggt ttgcagttat cggcttttgt gctataattg
    39601 ctataatata gtaaaaagca agaggagtgt gaggaagtgg aaaagaaaat tgtgaaggat
    39661 atcttatttt tatctcaagt gtctcagccg gcaagtcagg aggaccttta tcttgccaga
    39721 gatttgcagg atacactctt agcaaatcgt gatacctgtg ttggtctagc tgccaatatg
    39781 attggggtgc agaagcgcgt gattatcttt aatcttggct tggttcctgt ggtcatgttt
    39841 aacccagtgc ttctgtcctt tgaaggatcc tatgaggcag aagaaggctg tttgtccttg
    39901 gtaggtgtga gatcaactaa gcgttatgaa accataaggc ttgcctatcg tgacagcaag
    39961 tggcaggaac agaccattac cttgacaggc ttcccagctc agatttgcca gcatgagctg
    40021 gatcacttgg aaggacgaat catttaggag gaaagcaaat gaaacgaata gtctttgaac
    40081 ttatttttat cgcaacgacc tggtatatct ttttaccgcc ccttaacctg accagctggg
    40141 aatttctctt cttcctctgt gggcatttgt tagttgtggc aatattattt ggctttggca
    40201 aggggataaa ccttgtcaaa acggttcatg tgcgccacgg taaggcggaa gctgccttaa
    40261 atcttgaggg tttcaaaatc aatcggttag ggaaaattct gttagcttcg attggaggaa
    40321 ttcttctctt ggcagctttg gtttccttgg taacttccag catgtttcag gctaaaaatt
    40381 atgccaatgt agtcacggtt acggaaaaag actttactga atttcctaag agtgacacca
    40441 gtaaggttcc tatcctagat agaagtactg ctgaaaaaat tggagaccgc tacttgggtt
    40501 ccctaaccga taaggtgtcg caatacgtag cggcagatac ctatacccaa ttgacaattg
    40561 atgggaaacc ttatcgggtc acaccactag aatatgcaga ccctatcaaa tggtttaaca
    40621 atcaagccaa gggaatcggt gagtatatta aggtggacat ggtaactgga aatgcggatt
    40681 tggtggactt gaagacacca atcaagtatt cagactcgga gtattttaac cgtgatgtca
    40741 aacgtcacct gcgcttgaag tacccgacca aaatctttaa aactccatct tttgaggtgg
    40801 acgatgaggg caatcctttc tatgtagcaa cggtttacca aaagcaattt ggacttgctg
    40861 ttcctcgtcc tgcttcagtc attatcttgg atgctacaaa tggagaaacc aaggaataca
    40921 gcttatcaga tgttccagaa tgggtggaca ggatctatcc agcagaggaa accattgagc
    40981 aaatcaacta caacggcaag tacaaggacg gtttcttgaa tgccatgatt tccaagaaaa
    41041 acgtgaccca gactaccaat ggctataatt acttgtctat cggtaatgac atctatctct
    41101 acacaggtgt gacgtcggct aatgcggatg agagtaatct tggtttcatc cttgaaaata
    41161 tgcgaacagg agaaatcact aagtatagct tggcttctgc gacagaagaa tcagcccgtg
    41221 aatcagcaga aggtgctgtt caggagaaat cctacaaagc aaccttccca atcctcatca
    41281 acctcaatga caagcctctc tacatcatgg gcttgaagga caatgctggc ttggtcaaag
    41341 agtacgccct ggtagacgca gtcgagtacc aaaatgttat cgttgctact acagtggaag
    41401 agatgctcag caagtatgcc aataaaaacg accttgaaat tgacaatgca acgacagaaa
    41461 gcatcaatgg agtagtagca gacctcaaat cagctgttat caagggagac actgtctact
    41521 tctttaaagt tgatggcaag atatacaagg tcaaggcttc agtatccgat gaccttcctt
    41581 accttgaaaa tggtaaaacc ttcgaaggtc aagtaggaaa agacaattat ctcaagacct
    41641 ttaagctacg gtaaaaatag gtttttttca gaaagtatat gttataataa ggtaaattaa
    41701 gcctaatgga ggacaaagaa atgggttttt atttgatggt tgcttcaatg ctactcggac
    41761 tgctcgcttt gaagataggt ttttctcagt tcaaggagaa aaaagataaa ttcttatcta
    41821 tcttaacaag tttagctggc acagcccttg ttctcgtggc tgtctggtta ggatggccca
    41881 aataaataga aaaacctcgg tcaaaccgag gtttttcaaa tgaatttctt ggttgtggct
    41941 aaaaaatatg ctacactatc aatatgaaaa ttttaatccc aacagcaaaa gaaatgaaca
    42001 cagacttgcc aagtatcgag gcaattcctt taaaaccaga aagtcaggcc gtgcttgatg
    42061 ccttggctct ctattctgcc agtcaattgg agagtttcta caaggtatca gctgagaaag
    42121 cggcggaaga atttcaaaat atccaagctt tgaaaaggca aactgctcaa cactatccag
    42181 ccttgaaact ttttgatggg cttatgtacc gcaacattaa gagagataag ctgaccgagg
    42241 cggaacaaga ttatcttgaa aatcatgttt tcattacctc ggctttgtac ggtgttgttc
    42301 cagtcttgtc acccatggct cctcaccgtt tggatttttt gatgaaatta aaagtcgctg
    42361 gtaagacttt gaagagccat tggaaggcag cctatgatga aactctgaag aaggaagaag
    42421 tgattttctc tctcttgtca tcagagtttg agactgtatt ttctaaggaa atcagagcaa
    42481 agatggtgac cttaaaattc atggaggata gaggcggtca gctgaagatt cactcaacta
    42541 tctccaagaa agcgcgcggg gcctttctaa cagctttaat agaaaatcaa gtacaaactg
    42601 tgggggaagc acgtcgcttg aactttgctg gatttgttta ccgagaagat ttgtcacaac
    42661 cacagggatt ggtttttgta aaggaagtat agaaatcaga tattttgtat ttggcaatat
    42721 gtatcatcag ctattttagt ttataaatga gaaaaaagcg aacaagctag tcttctgagt
    42781 gtcagaaaag tagtcttgtt cgcttttatt atgttatact cttcgaaaat ctcttcaagc
    42841 cacgtcagtg tcgccttacc gtatatatgt tactgacttc gtcagttcta tctacaacct
    42901 caaagcagtg ctttgagtag cttgcggcta gcttcctagt ttgctttttg atttttattg
    42961 agtgttagtt acttgatagc ttcgaccaag tcttgagctt gtttttcaag tgagtttagg
    43021 actgtttctt caagaaccaa ttttccgtct gcccaggcag agtcattaac acgtgcagca
    43081 gtgaaatcac caacgccttg tgtacggata aatggcaaga ggtctttgta gatagcgaaa
    43141 agttgatcgt gccctgcatt ggctacagat gatactgtga caaacttgtc ttgaagggca
    43201 gaaacgccac gtgtatcaga caagtcaagg gcacgagata gccagtcaag caagtttttc
    43261 actgtaccag ggatagagaa gttgtagact ggagagaaaa tccagatagc atccgcaacg
    43321 agaactgctt cacgagcagc agctacagct ggatgtgttg gaacttccaa atcttggctg
    43381 aagagaggaa gggctgaata atcaaggtag ctaacttccg ctttaccagc aagtgctttc
    43441 tcagcttcga gcgccatttg gtggttgaaa gaaccttgac gtagtgatcc gacgataaat
    43501 aatacttttt tagacatgat gtgtctcctt tttcttaaaa tttcgagaac tctctcgttg
    43561 ttataaaata tattacaaca aaacaaaaga acttgcaaga aatttgctca gagtttattt
    43621 gaaaattgta aaatttcgga aactctagtc atttgtgact gaaaaagtaa gaactgtttc
    43681 actttcaaaa tcggttttgt ttgaagggag aaatagaat
//
LOCUS       DAVBNO010000013        43606 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVBNO010000013 DAVBNO010000000
VERSION     DAVBNO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.013"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(93..1319)
                     /locus_tag="VQ846_000762"
     CDS             complement(93..1319)
                     /locus_tag="VQ846_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET3075386.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(1391..2056)
                     /locus_tag="VQ846_000763"
     CDS             complement(1391..2056)
                     /locus_tag="VQ846_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET3075387.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(2068..2745)
                     /locus_tag="VQ846_000764"
     CDS             complement(2068..2745)
                     /locus_tag="VQ846_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET3075388.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATSLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVNRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            complement(2767..3000)
                     /locus_tag="VQ846_000765"
     CDS             complement(2767..3000)
                     /locus_tag="VQ846_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075389.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            complement(3286..4149)
                     /locus_tag="VQ846_000766"
     CDS             complement(3286..4149)
                     /locus_tag="VQ846_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET3075390.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRRVVDCFSHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            complement(4544..5716)
                     /locus_tag="VQ846_000767"
     CDS             complement(4544..5716)
                     /locus_tag="VQ846_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET3075391.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGTLP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTKQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFIKQSDLIVANRWDRDLEAYKDKVYTRGIWIRD"
     gene            complement(5729..6478)
                     /locus_tag="VQ846_000768"
     CDS             complement(5729..6478)
                     /locus_tag="VQ846_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET3075392.1"
                     /translation="MSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSKLKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            complement(6490..7991)
                     /locus_tag="VQ846_000769"
                     /pseudo
     CDS             complement(6490..7991)
                     /locus_tag="VQ846_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
     gene            complement(8059..8902)
                     /locus_tag="VQ846_000770"
                     /pseudo
     CDS             complement(8059..8902)
                     /locus_tag="VQ846_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8922..10538)
                     /locus_tag="VQ846_000771"
     CDS             complement(8922..10538)
                     /locus_tag="VQ846_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075393.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            complement(10625..11501)
                     /locus_tag="VQ846_000772"
                     /pseudo
     CDS             complement(10625..11501)
                     /locus_tag="VQ846_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375706.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(11494..12698)
                     /locus_tag="VQ846_000773"
                     /pseudo
     CDS             complement(11494..12698)
                     /locus_tag="VQ846_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            12941..13234
                     /locus_tag="VQ846_000774"
                     /pseudo
     CDS             12941..13234
                     /locus_tag="VQ846_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            13341..13635
                     /locus_tag="VQ846_000775"
                     /pseudo
     CDS             13341..13635
                     /locus_tag="VQ846_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13969..15275
                     /locus_tag="VQ846_000776"
                     /pseudo
     CDS             13969..15275
                     /locus_tag="VQ846_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(15315..16325)
                     /gene="tsaD"
                     /locus_tag="VQ846_000777"
     CDS             complement(15315..16325)
                     /gene="tsaD"
                     /locus_tag="VQ846_000777"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET3075394.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDDLAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(16315..16752)
                     /gene="rimI"
                     /locus_tag="VQ846_000778"
     CDS             complement(16315..16752)
                     /gene="rimI"
                     /locus_tag="VQ846_000778"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET3075395.1"
                     /translation="MIEIKRIQKHPDLAQAIYAVMIAVYPVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQSQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(16749..17432)
                     /gene="tsaB"
                     /locus_tag="VQ846_000779"
     CDS             complement(16749..17432)
                     /gene="tsaB"
                     /locus_tag="VQ846_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET3075396.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQILAEMTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVSLMDARRNNVYAGFYENAKPVMPEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQEYLPRTNFKETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            17610..17795
                     /gene="cibA"
                     /locus_tag="VQ846_000780"
     CDS             17610..17795
                     /gene="cibA"
                     /locus_tag="VQ846_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET3075397.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            17797..17949
                     /gene="cibB"
                     /locus_tag="VQ846_000781"
     CDS             17797..17949
                     /gene="cibB"
                     /locus_tag="VQ846_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET3075398.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            18755..18988
                     /locus_tag="VQ846_000782"
     CDS             18755..18988
                     /locus_tag="VQ846_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET3075399.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRIAARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFKITEF"
     gene            18990..20669
                     /locus_tag="VQ846_000783"
     CDS             18990..20669
                     /locus_tag="VQ846_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET3075400.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     ICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEILILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(20921..21718)
                     /locus_tag="VQ846_000784"
     CDS             complement(20921..21718)
                     /locus_tag="VQ846_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET3075401.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLDAETDVAVYGHVHKQLLRYGS
                     QGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKVAYDYEAELELAK
                     SKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDFL"
     gene            complement(21708..23552)
                     /gene="uvrC"
                     /locus_tag="VQ846_000785"
     CDS             complement(21708..23552)
                     /gene="uvrC"
                     /locus_tag="VQ846_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET3075402.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(23592..24293)
                     /locus_tag="VQ846_000786"
     CDS             complement(23592..24293)
                     /locus_tag="VQ846_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET3075403.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(24372..25604)
                     /gene="murN"
                     /locus_tag="VQ846_000787"
     CDS             complement(24372..25604)
                     /gene="murN"
                     /locus_tag="VQ846_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET3075404.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(25609..26829)
                     /gene="murM"
                     /locus_tag="VQ846_000788"
     CDS             complement(25609..26829)
                     /gene="murM"
                     /locus_tag="VQ846_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET3075405.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFVIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRISKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(26941..27720)
                     /locus_tag="VQ846_000789"
     CDS             complement(26941..27720)
                     /locus_tag="VQ846_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET3075406.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(27789..29450)
                     /locus_tag="VQ846_000790"
     CDS             complement(27789..29450)
                     /locus_tag="VQ846_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET3075407.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILHESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(29625..31847)
                     /gene="recJ"
                     /locus_tag="VQ846_000791"
     CDS             complement(29625..31847)
                     /gene="recJ"
                     /locus_tag="VQ846_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET3075408.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            32124..32882
                     /locus_tag="VQ846_000792"
     CDS             32124..32882
                     /locus_tag="VQ846_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET3075409.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAILSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     LDTTDVDNFFDNPSKPRAQQFLSKIINHESDKVK"
     gene            32893..33687
                     /locus_tag="VQ846_000793"
     CDS             32893..33687
                     /locus_tag="VQ846_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET3075410.1"
                     /translation="MKKKFFLSALLISLFGLAAAKPVQADTSIADIQKRGELVVGVKQ
                     DVPNFGYKDPKTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIA
                     TFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE
                     LGKKKGLKFKFVELGSYPELITSLHAHRIDAFSVDHSILSGYTSKRTALLDDSFKPSD
                     YSIVTKKSNTELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            33700..34377
                     /locus_tag="VQ846_000794"
     CDS             33700..34377
                     /locus_tag="VQ846_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET3075411.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            34387..35046
                     /locus_tag="VQ846_000795"
     CDS             34387..35046
                     /locus_tag="VQ846_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET3075412.1"
                     /translation="MESILEILTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            35283..36407
                     /locus_tag="VQ846_000796"
     CDS             35283..36407
                     /locus_tag="VQ846_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET3075413.1"
                     /translation="MEFSMKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSL
                     TFILATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNL
                     AIYIFASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFL
                     VGSYALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQF
                     AFLKIFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYG
                     HMIIEEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYNFRGDENAVYLDLLRNY
                     AQKNPNFELHLIDSTKDGYLNFEQKKCPNMQPSICVVLFL"
     gene            complement(36805..37686)
                     /locus_tag="VQ846_000797"
     CDS             complement(36805..37686)
                     /locus_tag="VQ846_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET3075414.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(37827..39155)
                     /gene="vncS"
                     /locus_tag="VQ846_000798"
     CDS             complement(37827..39155)
                     /gene="vncS"
                     /locus_tag="VQ846_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET3075415.1"
                     /translation="MKRTGLFTKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKNRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(39152..39808)
                     /gene="vncR"
                     /locus_tag="VQ846_000799"
     CDS             complement(39152..39808)
                     /gene="vncR"
                     /locus_tag="VQ846_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET3075416.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(39857..39940)
                     /gene="pep27"
                     /locus_tag="VQ846_000800"
     CDS             complement(39857..39940)
                     /gene="pep27"
                     /locus_tag="VQ846_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET3075417.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(40121..41500)
                     /gene="vex3"
                     /locus_tag="VQ846_000801"
     CDS             complement(40121..41500)
                     /gene="vex3"
                     /locus_tag="VQ846_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET3075418.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(41552..42199)
                     /gene="vex2"
                     /locus_tag="VQ846_000802"
     CDS             complement(41552..42199)
                     /gene="vex2"
                     /locus_tag="VQ846_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET3075419.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIQ
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(42212..43489)
                     /locus_tag="VQ846_000803"
     CDS             complement(42212..43489)
                     /locus_tag="VQ846_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3075420.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQVSLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT        13310 a         9486 c         7386 g        13424 t
ORIGIN      
        1 cacattcaga aaagagaaaa aagtctgttg attttgacca tcataaaaag actggcaatc
       61 cagtctcaaa catatattat agaaattctc cactaaatac tttcacgaat attcagaagc
      121 ataacaaagg caactagaag aaatagcaat aaaacaaagc taactgccag agttccaaag
      181 ctagtagcaa tggttaccaa agatattgta aataagctag gtaaaacaac cgtaatggca
      241 ctgatagaag attgaactgc tcccattgac tcctcaggta tttgtttaaa gacgagttct
      301 tgcaatctag gagagagaac acctgcgaaa aaggcatcca aggtactaaa gatgagaatc
      361 cagtcaaaac gaactgtggc aaatcctact agaagaagca actggatgac aagtgaggca
      421 tagagagctg tttttatgga aatggtatgt tgcagatagc cacttacaag gcttccgaca
      481 atcagggctg acagttcaaa agtggatagc aaggcaagag attgacctgt ttgtaaattc
      541 aaaaagggtt gattcctcaa aaatagagta gagacaggaa ctgtaacatt tatcactgct
      601 tgactagtag agataataaa caaaaccaag agcaccttat tcatattcca tatcaatttc
      661 gatgattgga gcaaatgctg gcaaaaggat tttacagaga gtccttcttg atagctaatc
      721 gttttttcta ctttcaagag gtcagttttt atgaagagga tacctaaaaa tgcgattaaa
      781 aaggtaagag cgttcagtaa ggaaataaac tggatggata gaatacctag taagactcct
      841 cctaggatat tactgattgt tttcactaaa ctaacagttg actgtttaaa tccaatagct
      901 tctgccagat gatcttgccc aataattcta atgaaaatcg gagtgagcat ggcgcctgaa
      961 aaataactca atgtgtcaga caagaggtta atcagacaaa taaaggttac tagcaacaag
     1021 gaaaaagact gccctgaaag cgatagggac actatagagt aaagcaaaaa ttttgtaaaa
     1081 ctaatgactg tgtatttcaa gacacgatga tgttgaaaat ccgccaaaac tcccagaaag
     1141 atttgtagaa cttggggaag ggtttctgaa atcgtgataa gtaaaatcgc caaaggggca
     1201 aaagacgcat ctgccacata attcaggaag gccagataaa aaatcgtatc cccaagcgtt
     1261 gaaatccact ggttgatagt taattgccta aaatctctat tttgaagaaa tactttcatt
     1321 acaactcctt tttaaattca aatgggaatc tttccccaag aatagaccgc gatactacta
     1381 acaaccaaaa ttacagtaac atcaaaagct gaccaatacc attgtagact atatgcagtc
     1441 caataggcca ataaattgac tttgtcattc taaataagac tgcaaatata agacctccac
     1501 ccatatagaa gacaaagtct gtcaagaccc aaccgtgatt actaatgtgc gagaccccaa
     1561 ataaaacagc ggaaccaagt acatctagcc cccatttctt tcctttttcc agagcagtca
     1621 tcactaatcc acgataaatc atgtcttcaa aaatgggacc tgcaatcaca ggataaaaaa
     1681 aatacatcaa aaatgccgta gctcctgtaa aagtaggagc agcatgttga taagaaattt
     1741 catttcgagt aggtgggaaa agaaaaaagg tgacaaaatt ccaagcaaca aaagcaagca
     1801 gagctaggaa ggaatagaaa agataggatc ctttaaactt tctactattg attttctgcc
     1861 atttccccga ccaaatcata gcaataagag caaataaaac cacaagaaaa ttcaacatca
     1921 tatccgacag ataataggca aagtcagata gcccagcaac aaggtcgctg cgtaaaacta
     1981 aaacactgaa cttctggtca gcaataacta gtagaaaaac tataataaag tagcggtgtg
     2041 agattatctt tttcatatat cacctttcta atatccaaat accaataaag taacaatgag
     2101 taagaagcta ttccatgaag catgcagagc tatagcccaa tagatggatc gggtgtagcg
     2161 aaacatcata caaaatatca agcccattcc aaaatacttt atgaaatctg tcgttatcca
     2221 accatactgc aaaacatgca tagcgccaaa tatggcagcg gaaacaagaa catcaagata
     2281 gtatctatta actttagata aacttgtcat caaaagacca cgacaaacaa cctcttctga
     2341 tacaggtgcg ataatactag tataaagtat tcgcgtaaca aaatagctaa ttcctgttaa
     2401 actggtggct acttctacga ctgtacttcc attctgggta cgaggaaaga tataggttgt
     2461 tagatttgcc cacacgaaca ataagaaaaa agaaagaagg aaaacaccca ggtaagacca
     2521 acgaaactgg aaacgaccac actctttcca atgttcactt ttgacaaaag caattgtagc
     2581 tatagttccc agaataagta ccaataaaac ttggaacaca tagtacatat tatcagacaa
     2641 agcaaccata aaatctaagt ctgatgtgac attaaaaatg aggtaataag tcaaaatcaa
     2701 caagccagtt gctaggtgaa atttcacttc tttcattttc ttcatcctat tatctcctat
     2761 aagagcctat cttctacggc ggccaaacaa tccatctgct aaatctatag tccaatcaaa
     2821 agctccacga ttaggactca tcccttgatt gccccaacca gggtaaattc ctgggacgcc
     2881 ccaaccagat ataccacttc ttccaccacc tcccatagaa tttacgaggt tgcctcctct
     2941 aacatcttgc aactcagctt ctgtcaattc cattgtttct gcaaattgta aatttaacat
     3001 cttttacact ccttcaatta tcttcatttg taaaccactt ctgcgaccta ggatttgctt
     3061 caaatgtttt acaaggacag tataacacgg aaattggctt attttagaaa atcgcatatt
     3121 tgatattttt tcttatagaa atttcagatt tgcgattttg gtagatttga ttatttccct
     3181 ggtataataa agttattact aatgaggagg gaagtaatat gaagaaacaa gttttaacat
     3241 tattgacgat cgttgctgat attattatct ttttcccatt tctaactaat cgataagctc
     3301 tttgtattgc tgcaaacgca attcaaaaag ggctgtcaat tgtggatttt ctaatacttg
     3361 cagagattgg ataaagtgtt caatctcttt ttgattgctt cctttggttt gaagaaaaat
     3421 ctgcattttc tttaaaaact gccacaatac tttttcaaaa acatcgtatg gacggagcat
     3481 gcgctccagc tcagcttcaa agattggaat ataagaaaat agcttgcgct ccataagttc
     3541 tgagatgata ttaagataac cacttttcat ataccatcga ttgtgtacta actctttaaa
     3601 ttccttggat ttttcgagta aggaggttga taaaaagatc agatcttgat tgctcaagaa
     3661 gggcatagta ttgcaaaaaa gatagagttc aaaccaggtc caagactcaa tagcgtagag
     3721 gtaggtagtc aaaaactcgc tatcctcctc tgttagcggg tagcttttat ctaaggaatg
     3781 gagtgcattt ttaatcacaa tggcattcaa acgacgatag gtctgcgcca tctgttcttg
     3841 atcgacttcc tccaatagct gctctaaagc agctatatcc tgatgggaaa agcaatccac
     3901 aacccttcta ccaattcgca tatgtggaga ttcttgatag ttgttgagtt tgtgcccaaa
     3961 ctcatcaaaa ttcatattga tcccttggat ggctaggatc agcttgtctg cagacagcat
     4021 agactgtcct agttcaaact tggacaactg agaggctgtt agtccagcac aagccacatc
     4081 tgactgcttg agctttctcg ccaagcgcaa ttccttgtaa aattccccca gttccatttt
     4141 ttcaatcatc ctaccacctc ctagcttttg catattatat cattatttgt gttcatttgt
     4201 caaaacattc tgacactttg gtgattgaaa aagccagcct taagctgact ctaaaaggac
     4261 cgagccttgt acaatacaga actcaatcct cgttctaaat agaatgtcta acttttttgg
     4321 gcagtacacg agcttgggga acagccactg gaacgatgag gtgtgagctc aaaatatcct
     4381 ccagttatgt ttttcctaat agtataccgg aagagtgaaa ggattttata atggagcggt
     4441 tacaaagaac ctactttcta ttaaacagta tactatgaaa atgtgaaaat ttaacatttt
     4501 tttgtacaaa ttttataaat tattgccttt ttaatatcaa tagttaatct cttatccaga
     4561 tcccccttgt gtaaacttta tctttataag cttcaaggtc cctatcccat ctatttgcaa
     4621 caattagatc actttgtttt ataaatagtt caaaattctt ttcaaaaatt acgttatcta
     4681 tactaacgtt taaatttggt tcatatacta aaatttttat accgacaatc aatagttcat
     4741 taattatact taaaatagct gactctttgt aattatctga attatatttc atccccaatt
     4801 tatatattcc tactatcttt ggctttcgtt ccaatatttg tttaactatg aactgttttc
     4861 tatttgtgtt tgaaatatca atcgcttcta tcactggggc atttatttct ataaattctt
     4921 tttttaattg tttagtatct ttgggaagac aatatcctcc aaatccaaaa gaaggattat
     4981 tataaaaatt tccaattctt ggatctaaac aaacaccttt tattacaact tcagcattta
     5041 aacttctcct ctcagcaaaa gaatctagtt cattaaaaaa agcaacacgg agagctaaga
     5101 atgtgttaga aaaaagctta attgcttctg cttcagtagg agaaactaac ataacatttt
     5161 taatattggc agtactatga gtactaatcg aaaggaacaa ctctgcaatt tttcttcctt
     5221 caactgtctc atctccaaca actatgcgac ttggatataa attatcatat atagaacaac
     5281 cttctctcaa aaattcaggg acaaaaatga tattttttgt atcaaacagc ctttttaatt
     5341 tgtttgaaaa gccgatcgga actgttgact ttaaaataat ctttccatta ggttttaccc
     5401 tcagaatctt cgataccgtt tgttcgattt catatgtatt aaaactacca attttctcat
     5461 cataatctgt cggaagcgca ataatataat aatcaatatt atttttaatt tcagaaaatg
     5521 tatcaaaaaa agtaatattt aagttattct cgcaaaaaaa cttcataagc tcttcatttt
     5581 tagatggaag agtgcccttt tttaaattat ttatttttac agaatctata tcatatgcaa
     5641 caactttata tttagatgca aatagtaacg cgtaggccag cccaacatgc cccaaaccaa
     5701 ttactgctat attcataaaa ctacttcctt atttcttaat ccaaaatcta atagaataag
     5761 ctgccccatt ccttaaatac aactctttaa tattgtttaa aagtttttca actgatttcc
     5821 agattatcaa aatctgagat ttatagcaca atattgatga tattctatca atataatgtt
     5881 tttcatcaag ttcctcttga tacattttta attctttagt ttttcccata taactaacca
     5941 tactactatc acttacatat gggaagtcct cataatatat tactttataa cgcataaatt
     6001 caagcgccct tccaatacta ttcacaaaaa catgatcaac atggtcacca agtgaaagcg
     6061 gacaatatac gacacatttg tcgtctaaat gcattaacag ctcttttatg atatcattct
     6121 ttaatgtgtc ctcatttttt aatttactat agatatgacg gtatagaaaa ttgccatttc
     6181 tatctttcct atagagacat tcatagtacg ataagtgtct aaaatcacat tgtagacgtt
     6241 cacaagctaa cctgtcttct ttcttccttt cttcaatcgg atatttccca aggttacaca
     6301 acttatgaaa ttgcttagca gagggctgta gctgttggct caaagggtaa ccagaaaata
     6361 tagtaataac aagtacaatt tctccttctg aagttaattt tgaaatataa tcaccacagg
     6421 aaaaaattgc gtcatctaaa tgtggagata aaaagatata cttagtattg tcactcataa
     6481 ccattccctc tacaatttat ctaaaaactc actaagtgtc tgattaaatt ccacatcatc
     6541 aaaaaaattc accttattct taataatgaa tatttcgtta aataaacata tatataaata
     6601 tttcaatatc ctttcaatat catcctctaa attctcctca atattttgta tcagcccatt
     6661 tacaatctta ttaaaaaaga taagctcttt atctctaaaa ttaaatattt tcatacaact
     6721 gttgtatcga aaaatatata aaataatttt tactaatgtt tgaatattta aacaactaaa
     6781 taaatgagtt gtacccggga cactatttat gttatcaaga acactatctt gaaacctcaa
     6841 ctcacagttc tttttgtgaa attctttttt atcgtttaga tctgatattt ttttagacat
     6901 ttcaacaatc tcagacattt tatatggata tctaggatga atgccaaaac tatgcaaaag
     6961 taggctattt ttctttaaaa caattgaatt tgtaacataa aatggatagt cggaataact
     7021 aaaatatact gacttctgtt tatcattaca aaattgattg tagttcacag aatgatactt
     7081 aaaagtcatt atttcatccg ctgactcgcc acaaagaata cttgtgttcc ctttttggaa
     7141 caacaacctg cctaaatagt actgcagtat gacaccacat tcaaagagac ttccttccaa
     7201 tctccatact atatcgggaa aatattctaa atcttgggct cattacagta tcaactaata
     7261 aatttatacc ataaaatttt gcaattttag ttacttcagg aatctcatta attccttttt
     7321 ctcctcctat gcaaaatgca ttaattgaag aattcgtata attcgctaat gtatataata
     7381 tataatttga atcaaaacca gatgatagtg taatattagc ctctcctgct tcactataca
     7441 ctcttaatat agattcacgt agcatagagt cccaattctt taatacttgt tctttagtaa
     7501 aattgtttga atcattataa tcaataccac atatacgaag agtattattt acagaaatgt
     7561 aagagaaata ttctaattta ttaatttcgg ttactaaagt atttgaatct aaaataaatc
     7621 catttctcat aaattcttgt atttttttgt tatttaaggt aacgggaaca tgagataact
     7681 tcatcacttt ctttaaagat gtactatata tagaacacac ctccataaac agtatagtat
     7741 aaattaaagt atgaagtcgt tatatcttga aaaattttta attcttttgt cttcgtatca
     7801 aaaattataa ttaaaaacat tccggtaata ttctttatta agctaattcc ataaatctta
     7861 tacaatccag cgaattcatg caattccaac tttatatttt tataataaat ttctccaaga
     7921 agttcaattc ttattttatc tgtttcaaag atatcagtta aagaataact aattagattc
     7981 ctagtttcca tatcgaagct tcctgtgata ttcaataaag tattttccat atttctttat
     8041 ctctctcgaa tagattaact attctaaaaa atatttaata agtttacttt tataatcttg
     8101 taaaccataa caaaaagaat aatttctatc taaaaaatga catctactaa tctcttctac
     8161 atatacgtat tttccaatag aatctttccc aaacatacta tcccaccaaa aaatacaaaa
     8221 gtattctata ttagtaagat cgctataata gataattcct ccagcaacaa tttttttcca
     8281 aatagcaacg aaattgtttt aaaatttttt tgattatata aaagcttaaa taattcaaat
     8341 tggctttcag taaacgtaac cgaaaatttt cgattgcaat aatttttcca gttttgcagt
     8401 atatctgaaa cacgactgaa attataaaca caaacatatt ttccgttatt tttatcaacc
     8461 agtgttctaa aacgcttgta aaaaacacca aatttagagt catctgaaac atctacaata
     8521 ataagctctt ctctatgaca caaagatata ctttgagttt ttaggatgag atccatattt
     8581 ttatagcaaa aatcgataaa attcttttca atttccaaag aaaagtgatg cagcaacgtt
     8641 ggatcaactt gcttattgga aactccatct aagattggga tattttttta gctagaacta
     8701 gtagaaatat atagtcaaat aacagatacc ttaagggttt ctcatataca taaaaaaatg
     8761 atactttttt cttatctaat aatttctcac atattataaa caattcttcg acagaggaat
     8821 acttaacttc taactcaata tcattaaagt atactttttt ctcttcagta attacctcat
     8881 aagcttcaca atagaatctc atgtttccct cccctatatt cttaaataaa atcctttgga
     8941 aattgatata tcttagtaaa atattgttta agttccggat gcggagcatg ggtaacaata
     9001 atgacagtca aatcctctct atctaatatc ttacgttcaa tcgctaacga agttctccta
     9061 tcgatagcag aagttccctc gtcaattaat actattttct tatttctaat tagccctcta
     9121 gctaaagtaa ttttttgttt ctgccctcct gacagtaatc tcccatcatc accaacataa
     9181 taatctaaaa tgttattagg aaaatctttt acactcaaac caacttgctc taaagactgt
     9241 agtatttctt catcagtata attttcttcc aataaaatat tatctctaat cgtaccttca
     9301 aacaaataag ctttttgatc tacatataga acattcgaaa ccatatttaa ataggaggtt
     9361 ttttttatat catccccgca gaatcgcaat tctccactat aatctctcaa aaagccattc
     9421 aataatttta ataatgtaga tttcccgctt ccactttcac ctaaaattaa atacttttca
     9481 ttacgttgaa aacaaaaatt taagtttttt aatatttctt tatctccata cttatagcaa
     9541 atattttttg cttcatataa cggaaaatct ctattcacct catttggttc gatatcattc
     9601 attttatttg actcaattgg attaattgaa tacaatttta aaaaaatagg cttcgtacca
     9661 ataatagagg ataattgacc tcctaattca cctagcgctg taaaaataac acctgttagt
     9721 gctcctattg cttcaatagt accaattttc actattcctt ttattgcaag atagcctgtt
     9781 aaaaaaacga gagatatctg aaaaaaaata ttgagaaaga agctaatagc gcctgctaac
     9841 gtttctacag ttgtctttct ttgtataacc atctttaata aaattcctgc ttctttaatt
     9901 ttcttaggca atacatataa aagattcaag gacgctaaca catcaaatcc attcaatata
     9961 gtctcactag attttaaaaa agcttcattt tggttagtta aatttagact aacttctcgc
    10021 attttcgatg caaagatttt tggtacaagt agcataatca ttaatgaaaa caaggtggct
    10081 acagtcaatg accaatgata gtgattaaga gtcacaactg caaatatagt accagaaatt
    10141 ccttttatta ctaaaaaaag ttgtttaaaa gcctgatcat ttaaagtctg aacatcatta
    10201 tttagccacg aaagatatgt tcctgatgat ttactatgaa attcttgata ggtagagtta
    10261 gagatgtctg tggcaactct atttcgaatc tctagattaa actcttggat cacttcaacc
    10321 tgataatttt tcactaccca gtcaaggaat attatcccac accagacaat catttggtag
    10381 attgacaatt tcaaaaaccg ctctaaattc atcgcaatta attcattcaa caccagagca
    10441 ttaatagttg ctgcataaat tagcaataat tgaccagcaa caataaatat cgttaataaa
    10501 ctaaattttt ttatatttga ttttataata gtatacacaa tagtttctca ctttctaaat
    10561 tttaattgaa catagttgtc atatatacaa tagaaaaaac caaaatgata taataacata
    10621 tattttaaaa aagaaattcg ttaaaaattt tttcttctct tgccttcttg attactttta
    10681 aagccttgca tttgtctcct attaaatagt aaccgcttta tgtttaaaga ataatatttc
    10741 tttgtaacca atattctctc gttgaaactc aataaattaa aatatttcct acagtaatta
    10801 taatattctt catctgcatt aattgttttt tgtgtcactc cagtgatacc gttttcttta
    10861 ctgtgagcgt agtaattcac caagaattct cgcactatat caatttggta tccttgagca
    10921 agtagtttta ataaaacaac accgtcctga tgtgaatcta ttttctcaaa accattaatt
    10981 aattctagca cctctttttt acacaaccaa aatgacgtac ctgctatatt gtgaaccatt
    11041 tgaacaaaca agggatttcc aacaaaatcg gtcttctcct cttctcgtgt accatttgga
    11101 taaattatta ttccataact ataaactaaa gctaaattct tcattctact ctttttaaaa
    11161 caagccatca actttaaaat tcgatctggc atatattcat catcatcgtc taaaaatgat
    11221 atatacttac ctctagaatt tttgatacct atgtttctgg cattagttgc acctaaatct
    11281 tcattactta aaattaactt aattctatga ttggtatagc caaattgatg gataatttta
    11341 tttcttaaat ttacattact ataattatca tcaataatta taacttcgat atttttataa
    11401 ctttgatgta aacaactttt cacagctcta atcagagatt catacctatt atgtgttggt
    11461 attataatac ttactaattc ttgatctata ttcctatcca tgactactct tctctaataa
    11521 ttcatcatat actctcatgg tttctacaaa cattttttgc acagaaaaat gttttcttat
    11581 ttttgattta ctattctcac ctacatattt caaatactca gaatcattga gtaaaaaatt
    11641 agcacaagca cacactccct caacatcttc cttctcaaat aaaaatccat caaccctatg
    11701 ttcaataatt tcacttaacc cgccaacatt actagctaaa accggagttc cttgtgacat
    11761 tgactctaaa acacacatag atattccttc tgtatcagaa ggaatataca ataaatccga
    11821 tatttggtaa actatagtac tggatagatt tcaccaagta acctgaaatt atctctacat
    11881 ttcaaatagc aaattttttc tttcaaagca gcccacatac taccatttcc agccataata
    11941 aaaatcacat cttctctgac taaaaataat ttttctgcaa attcaaggaa tctatccggc
    12001 cttttttctg gatccaacct tccaacataa caaatgattt tttgttattt ggaatacaaa
    12061 attctttttt aaagtcttga acacctacta catctaaatc gctatttgat acattaattc
    12121 cgttatttat tgcaactatc ttcttatttt tttattatac tctccaatct ttttttcata
    12181 gtttcagata cacaaataaa agcatctccc atagaatatg tccaaaaatc aaaataagtc
    12241 aagaatttct tttttaagtt atattcaacc catccatggc atgttatcac tgtcttaacc
    12301 tttccaaatc cattcttgtc aagttttttt aacatatata aaaaataatt agttgagtag
    12361 ccatgacagt gtataagttg gatttttaat aattttaaaa tatttttaac gtgtaaggca
    12421 gtttcaaaat tatttgaaca ttgagtacaa tcaacatagg caatatctaa atttttataa
    12481 tcatcaataa cctttgaatc tctagatacg attatcaaaa taggatatag agacatcctt
    12541 ctgatctgct ttttacaaag tccaattata tgcggatcta tacctccaca atgtccatta
    12601 ttatacctaa ctataatatg agccgaaaac actatatcct taatgtctcc atatccatca
    12661 gggatattaa tatttatttt tccacaacta tattgcattg taaccatctc cttaaacgac
    12721 gcattatgat atttgataga gaaattttta tgaataactc aataatttta tagtaaatca
    12781 tgcttatatc tcaaagatac ctattttatc ttgtctcgac cttctccaaa gaattgctat
    12841 aatactatta caaatacatc tgcactacac ttcaaatttt agcactgtat aaaaacgttt
    12901 caatacacta acttcaaaaa aacttccact attaattgaa aaaattgata gagataaatt
    12961 aaaaatctat attgaaactc atcccgatgc ttatttgact gaaatagctg ctgaattcaa
    13021 ctgtcctcca acaactattc attacgctct aaaggctatg ggatatagtc taaaaaagag
    13081 ccgtacctac tgcgaacaag acccagaaaa agtaaatcgg ttccttaaag aattgaatca
    13141 cttaagctac ctgactccta tttatattta tgagacaggg gttgagacct atttttatct
    13201 cgaatatgat cgagccttga gcaggcagtt agtctctctg gaagaagata taattatttg
    13261 aattaagatc gagacaacgc acaccagaga ttgcgatact gttatagaag tactaatgcc
    13321 cttttttgtt tcaatatact atggctccga tgacctataa agatacgatg acgagtgact
    13381 ttttcgaagc ttgcttccaa aaattcttac tacctacttt agatacacca tcccttatca
    13441 ttatggacaa tgcaaggttt cacagaatga acatgtgtaa ggagcagggc atagactgtt
    13501 accacttcct acctattcac ccgagtataa tcccattgag aaaatatggg cttacatcaa
    13561 aaacatctca gaataatatt gtcaaattac gatgcttttc ttgaggcact tttgtcctat
    13621 tcttgtttca gctgattata ctccgttatt gggcagctac ggaacagtcg atgggacgat
    13681 gggggggaca taaaaaaatc ctccagtttt gttttttata acagtatact ggagaattga
    13741 caatctcggt agatacctcg ttatagcgcg gttacttatt aggcagttac aaaacaactg
    13801 tgaacagaaa acattccaga gtcagacaag actttggaat gctttggctc tataatttct
    13861 gtagtgggta atcccacccc aggaattata gggtcgtttc ttgtagaaaa aaagccccat
    13921 atgacctata atgaaaagcg tctaaccaac tcattagaaa gggttcatat ggaacaactt
    13981 aagaatacca cagatttgct cggattggaa gacaaaaata tcaaaatctt gtctgttctg
    14041 aaataccaaa cccatctagt cgttcaggca aagttggatt cccccgctcc tccttgtcct
    14101 cattgtcaag ggaagatgat caaatacgac ttccagaaag cctctaaaat tccgcttctc
    14161 gactgtcagg gtttacccac ggtactgcat ctcaaaaagc gccgctttca gtgcaagaat
    14221 tgccttaagg tggtcgtttc tcaaacatcc attgtcaaga aaaattgcca gatttccaac
    14281 atggtgagac aaaaaatcgc tcagctcctc cttgaaaagc agtctatgac tgagattgcc
    14341 cacagattgg cgggctcaac ttccaccgtc atccgaaaac tgagggaatt taagtttgaa
    14401 accgattgga ccaagttgcc aaaagttatg agttgggatg agtatagctt caaaaagagc
    14461 aaaatgagct tcattgccca agattttgag tccaaatcca tcctcgcaat tttagacgga
    14521 cgaactcatg cggtgattcg aaaccatttc caacgctatc agagagaggt tcgggagctg
    14581 gtcgaggtca tcaccatgga catgtacagc ccttattatc ggctcgctaa gcaactcttt
    14641 ccaaaggcga agattgttct tgaccgcttc cacattgtcc aacatctgag ccgagctatg
    14701 aaccgagtac gaatccaaat catgaaccaa tttgaccgaa aatccttgga gtatcgggcg
    14761 ctcaagcgct tttggaaccc tcgctttttc gtttctaggc tcgggctaaa tcagtccact
    14821 ggactgattt actacaccag gatagcttca agctctgtca gaaacgattc tatcagccca
    14881 cgtttcgaat gcacttaacc catcgggaag tacgagataa gctgctttct tactctgagg
    14941 gattacaggt tcactacgaa ctctatcaac tcctgctctt tcattttcaa gagaagaatg
    15001 ccgaccattt ctttggattg attgagcaag aactgccaac ggttcatccg ctttttcaaa
    15061 cggtcttttg gactttttta agggatagag ataagattat caacgcactt aagctgcctt
    15121 attccaacgc taaacttgaa gcgaccaata atttgattaa ggttatcaag cgcaaagcct
    15181 ttggtttccg gaactttaac aattttaaaa aacggatttt gatgactttg aacatcaaaa
    15241 aagagagtac gaatttcgta ctctccagat tgtagctttt cgcctaccca ctacacttga
    15301 caaagagcca ctctttattc catggtatca aaggcaagac ttggtttggc attgaggtcc
    15361 cagcctgcga agttttcttt gttccactcg ctgacgctgg cataggcaat catacctgca
    15421 ttgtctccgc agagtcgcag aggggggatg ataaccttga catctgtgat ttcggctgct
    15481 aggcgttctc tgagaccttt attggctgcc acaccacctg ccacaactag ggttttaaca
    15541 ggatatttct ccaaagcctt cttagttttt gccatgagaa tgtccataac tgctgcttgg
    15601 aaggaagcac acaaatcttc tgtagacagg ctttctccct tttgctcggc attgtgatga
    15661 agattgataa aggcagattt caaacctgag aaggagaact ccagattatc ttccttaatc
    15721 atggcacggg ggaaatcata aatatcctgc ccctgatgag ccagatcgtc aatctcacga
    15781 cctgcaggat aggtcaagcc catgacacga ccgaccttgt cataagcctc cccaactgca
    15841 tcgtctcgtg tctccccaac aatcttgtaa tcgccagcct cagaaacata gaccaactct
    15901 gtgtgcccac cactgactaa aagggctagc aagggaaact ccaaaggctc cacactctga
    15961 gctgccatga ggtgcccagc catgtgatta acaggaatca gtggaagtcc gtgagcccaa
    16021 gcaaaggcct tggcagctga caaaccaact agcaaggctc cgaccaagcc tggtccgtag
    16081 gtaaccgcaa cagctgtcac gtcctcttcg gtaatccctg cttctgccaa tgcctcctcg
    16141 atacaggctg taatgacctc gacatggtga cgactggcta cttcgggcac tacgccacca
    16201 aaacgtttgt gactctcaat ttgactagca atgacattgg acaagagctc atcgtcgttt
    16261 ttcaagacgg cgacactggt ctcatcacag gatgtctcaa atgctaaaat atatctatcc
    16321 ttcatctatt tctctcttca tgataatggc gtcctcgact gggtcatggt agtaggcctt
    16381 tcgctcagcg ataactgcca tcttttcttt cttgtaaaat gcttgcgctc gttgatttga
    16441 ctgtctgact tcgaggaaaa tttccttgtc tgtcggcaat tgagcaaaca aggctgacgc
    16501 aatcccctga ctctgataag ctcctttgac agcgatttgc aggacttctg cttcaaaaag
    16561 attctcctgc acagctagaa atccaatcac ttctgcccca tcataagcca aagcatacca
    16621 agtctggtct tgggacaggt ctgcttggat ttgttccagc gtccaaggac tgactgggta
    16681 aacagctatc ataacagcgt agatggcttg agccaagtca ggatgttttt ggattcgttt
    16741 gatttctatc ataagcgttt aatgtaagac tcgccagact cggtgtgatt cttgagccag
    16801 ttttcctcag cttcgacacg cttgaggtag ttcggcacaa aatcatgcaa ggagtctgct
    16861 tccttgtccc aggcccatag agctagatta gctgcatttg gcaaggtttc tttgaaatta
    16921 gtccttggca agtattcttg aatctgctca acaaaggggc caacttctcc gacaaaggta
    16981 acctgactag cacccttgat taactcaatc actcgctcaa agggtaagtg agcttctggc
    17041 atgacgggtt tagcattttc ataaaatcct gcataaacat tgttgcgacg cgcatccatc
    17101 aaagagacga acaagccttc ttgttggtag ggtaccagag ccaagaggct agacatacca
    17161 actaactcga tgttcagagt atgagccaaa gtcttggcag tggctaccgc aattcgcaag
    17221 cctgtgtagc tacctggccc ttcagccacc acgattcggt ccaaatcctt gggtgtccag
    17281 tccaaacttg ccatcaaaaa atcgatggca ggcatgaggg taatactgtg atttttctta
    17341 atattaatcg tcatctcggc aagaatctgc ttatcctcta aaatagcgag agaaagagcc
    17401 ttgctggacg tatcaaaagc taatactttc ataacacatt cctatctttt tgtctgctta
    17461 ctattatact acaaaagctg gcacatggga attttctttg cccccagaca agagtgccct
    17521 cacttaacta aaaataattt aaaaaaatgc tcacttttcc ttttcttttc cgaatataaa
    17581 agtgaacaag aaaaaaggag gaaaattcaa tgacaaattt tgacattctt gacaatcaat
    17641 ttttatcctt atctgaaaat gaattatcag atattgatgg cggtctcgct cccttggtta
    17701 tctttggagt agcagtatct tggaaagcta ttgcaggtgg aacagcactt ataggttctg
    17761 gtttggcagc tggttatttt ttaggaggag attaatatga tgaaaaattt gaacaactat
    17821 cgtgaaattt ctaataagga attgcaagaa atcaagggtg gctttggtgt aggtgttggt
    17881 atcgctttat ttatggcagg ttataccatt ggaaaagacc ttcgtaaaaa gtttggtaag
    17941 tcatgctaga taagaaacac atttttagaa ggataaattt tattgtcttc atctcttaca
    18001 gtttgctcag cattctcaat gatttgaaca ttactactat ccctttacca ttcgatttat
    18061 ctgtttgtat tgttttattt ttatgcttca actctatttt tgagcagaac aatgactccc
    18121 ataaaaataa taagctttga aaattccatt gtcatgtcat gttagaaaaa tgcaaagacc
    18181 acctcatctt gatagatggg gtggaatttt cgtgtcgtaa atctactatc tctacatccc
    18241 caaacaaaaa accccagcat aagcagggca atccaaagta aaatacaaac caaacgacat
    18301 aggtcacgag gaggagaaaa agcgagtaga gagtcacaaa ggtaattttc ctcaagaact
    18361 tggtttgtca tcgttccagt ttggcaaata gaagatttcc tccataaacg caagcaacaa
    18421 aaacgataaa agctagcaaa cgaactccga tagcaaaagc aaataagtta tacatagggc
    18481 aacctccttg acttaaaatc tatatggaat tatgacaagc aataaatttc acttccgtta
    18541 tcaacataat acattttctt tgtttttgca aacgcttacc aaagaaatcg tcccctaact
    18601 ttctcgtttc cgtcttttac taatttttca ttttgtggta taattgaaat aattgtaacg
    18661 aatcaaggtc aatctagaca caaaatggaa tgaaatcaag caaatctctg ctaaaagttt
    18721 ggaataagct gacctgtaaa tagaaaggaa ctatatgatt tacaaagttt tttatcaaga
    18781 aacaaaagaa cgtagcccac gtcgtgaaac aacacgcgcg ctttacctag acatcgatgc
    18841 cagctcagaa cttgagggtc gtatcgctgc tcgccaactt gtcgaagaaa atcgcccaga
    18901 gtacaatatc gaatatatcg aactcttgtc tgacaaattg ctcgattacg aaaaagaaac
    18961 tggcgccttc aaaattacgg agttctaata tggcctacac tcttaaacct gaagaagtcg
    19021 gcgtttttgc catcggtggt ctaggagaaa tcgggaaaaa cacttacgga attgaatacc
    19081 aagacgagat tatcatcgtc gatgctggga ttaaattccc agaagatgac ttgcttggta
    19141 tcgactatgt cattcctgac tactcttaca tcgtagacaa tatcgaccgc gtcaaggctg
    19201 ttttaatcac acacggacac gaggaccaca ttggtgggat tccgttccta ctcaagcaag
    19261 caaatgtccc tatttatgct ggaccgcttg ccttggcttt gatccgtggg aaactcgaag
    19321 aacacggcct cttgcgcaac gccaaacttt acgaaatcaa ccacaacacc gagttgacct
    19381 ttaaaaatct caaggcaact ttctttagaa cgactcactc tattccagaa cctttgggaa
    19441 ttgtcatcca tactcctcaa gggaaaatca tctgtacagg tgactttaag ttcgacttta
    19501 ctccagttgg agaacctgcg gacttgcacc gtatggcagc cctaggtgaa gatggagtgc
    19561 tttgcttgct ctctgactcg accaatgctg aggttccaac ctttaccaac tctgaaaagg
    19621 ttgtcggcca gtccatcatg aagattatcc aaggtatcga gggacgtatc atctttgcat
    19681 ccttcgcctc aaatatcttc cgtctccaac aagcaacgga agctgctgtt aagactgggc
    19741 gcaagattgc ggtctttggt cgctctatgg aaaaggccat tgtcaacgga atcgatcttg
    19801 gctacatcaa agctcctaag ggaaccttta ttgagccaaa tgaaatcaaa gactatcctg
    19861 caggtgagat tctgatcctc tgtacaggta gtcaaggtga acctatggca gccctctctc
    19921 gtatcgccaa cggaacccac cgtcaggtac aactccaacc aggcgatacc gttatcttct
    19981 cttctagtcc catccctgga aacactacta gcgtcaacaa gctgattaac attatttctg
    20041 aagctggtgt cgaagttatc cacggtaaag tgaacaatat ccatacatct ggacacggtg
    20101 gtcagcaaga gcaaaaactc atgctccgct tgattaagcc aaaatacttc atgcctgtcc
    20161 acggtgaata ccgcatgcaa aaagtccacg ctggactagc agtggatact ggtgttgaga
    20221 aggacaatat ctttatcatg agcaatggcg atgtgcttgc ccttactgct gactcagctc
    20281 gtatcgcagg tcatttcaac gcccaagata tctatgtcga tggaaatcgt atcggtgaaa
    20341 ttggcgcagc tgtcctcaaa gatcgtcgcg atctatctga agacggtgtc gttctggcag
    20401 tcgcaactgt tgacttcaaa tcgcagatga ttctatctgg tccagacatc ctcagccgag
    20461 gctttgtcta catgagagag tctggcgact tgattcgcca aagccagcgt atcctcttca
    20521 atgccattcg tatcgcactg aaaaataagg atgctagcgt gcaatctgtc aatggtgcca
    20581 ttgtcaacgc tattcgcccc ttcctctatg aaaataccga acgtgaaccg atcatcatcc
    20641 cgatgatcct cacaccagat gaagaataat actcttcaaa ccacgtcagc ttcgccttgc
    20701 cgtatatatg ttactgactt cgtcagttct atccacaacc tcaaaactgt gttttgagct
    20761 gacttcgtca gttctatcta caacctcaaa gcagtgcttt gagcaaccta cggctagctt
    20821 cctagtttgc tctttgattt tcattgagta taagatatac taaaagcgat tggaccagcc
    20881 aatcgctttt ttagtttgca ttggctattt taggaaactc ttacaaaaaa tcaaaaaaat
    20941 tcttcacatc ctctacatac ccatgctttt ctattaagct ggctaataat tccagattgt
    21001 gcccctgata gttatcgtca cgacgcagtt cttcatacat ttcgataaag ggaagcccct
    21061 tggacttggc caattctaac tcagcttcgt aatcataagc aactttacga aattggatat
    21121 tgagtaattc cccatcttca acttctatca cggcatactg ggcacggtga ttttttaacg
    21181 cctcccaatt aaaatagggc atgccaatcg accctggatt gatgatttgt tgcccttgac
    21241 ttccataacg aagcaactgc ttgtgaacat gaccataaac tgccacgtcc gtttccgcat
    21301 ctagcaagtc accaccatag tttttgtcag gtaaattatg agagatagaa aagcgcaatc
    21361 cgtcaatttc tttcttttcc agcaaaggca agcttcgtag ccagacaatc gttgcaggat
    21421 ccattcgctc catcaaatac tgtgtcatac gcaagagctg aacttcctgt gggtcttcta
    21481 agccatattg cccatctaaa gcctcaagga cacgatcatc ccaattgcct cgaacacttg
    21541 ctgtgatagg aaggtccttt agcagggcga ctaagtcatt tgcgcctgga ccaggaagaa
    21601 aaatatctcc cagaagccaa tattcactgg ccccttgatt tttagcatct gcaatcactg
    21661 cttctagcgc cgtcgcattg ccatgaacat ctgataaaat tgcgatttta tggttcattg
    21721 tggtttcctt ccgtttggta atctactctt tcttctgcta cttgaggcaa ggcttctccc
    21781 tgcgggttca actttctttg cacagcctct gcaacgactc taggtacccc aacttcgaca
    21841 atctcatcca cactggcttc cttgattttg gtcaaagact tgaaatgcct catgagattt
    21901 tgcttgcgtt taggtcccag accatcaatc ccatccaatt gagatgagaa agaatttttg
    21961 gagcgcagtt ggcggtggaa agtgatagca aagcggtgca cctcatcttg gatgcgttgg
    22021 aggaggaaaa attcctgaga attgcgagac aagtccacca cctcaagcgg atctccaaag
    22081 agcaattcat gggtttggtg cttatcattc ttttgcagcc cagcaattgg aatatccaag
    22141 cccagttcct cttggataac ctgcttagcg atattgactt gaccttgccc cccatcaatc
    22201 acaatcaaat ctggaggagt caaagcctca cgctgtactc gaccatagcg tctgcgaatg
    22261 acctctctca tgctggcata gtcgtctggt ccaacaaccg tttttatctt gtacttacgg
    22321 taatccttct tactcggttt accgttgaca aagaccacca tagccgaaac agggctagtt
    22381 cccatgatat tagagttatc gaaggactcg atacgtactg gggtcgggat ttggagcaaa
    22441 cgccctagat tttcaatagc tccttgagtc ttttcgacag atttttctag cagattgaac
    22501 ttctgctcta gactaacacg agcatttttt atggctagat tgaccagttg ttttttctct
    22561 ccacgttgag gcttaagaat cttggaatcc accaaggcct tgacagcctc ttcgtcaata
    22621 tcctgcggaa tcagtacctc attgggaact agatgagatt tttcttgata gaattgtcct
    22681 acataggtca aaaaatcctc atctggatca ttgaagtagg ggaagagatt gacatcgcgc
    22741 tcgataagct taccctgacg gacaaagaaa acctgcacac acatccagcc cttatccaca
    22801 tagtagccaa agacatcgcg attttgcaaa tctttcgcca tgacccgttg cttggttcga
    22861 agcgttccaa tagcctgaat caggtcacgg tattccgccg cacgttcaaa ctccatactt
    22921 tgtgctgcta ctgccatttt actcttgaga tcatcgatga ttttgtcatc ctgacctttc
    22981 agaaaatcag acacctcctg agccatagac ttgaaataag cctcatcctt cttacagatg
    23041 gtgtgggcca tacactggcc gatatggtaa taaaaacaga ccttagaggg cgggttggta
    23101 cattttcgaa aagggaaaat cctatctaga agtcgcttga tttcattggc tgcccccaca
    23161 tctggataag ggccaaaata aagaccaccg tcctttttga cctgacgagt aatgataagc
    23221 cgtggatagc gctcattggt gattttgatg aaaggatagg acttgtcatc cttgagcatg
    23281 atattgtact tgggcttgtt ttccttgatc aggttgattt ctaggagaag tgcctcaata
    23341 ttagactccg taacaataaa ttcaaaatcc acaatttcag acaccagagc ctctgtcttg
    23401 gtatcatgac ttccacgaaa ataggaccgt actcgattac gcagattttt agcctttcct
    23461 acatagataa tggtgccatt tttatcctta tgaatgtagc aaccagggct ggtcggcaag
    23521 agctctagtt ttgatttaat caagttgttc atagttccat tatagcaaaa aaagtaggga
    23581 aagatagtac cctactcact agtctcatat atttttcgga ggtttgcaac atcctcttcc
    23641 tggataccat aaaaggaacc tgctggttgc atgacagact tctcatctgt atggtccaag
    23701 ccaatcgcat gacctaactc atgttctgcc gtatggacaa ggcgttcata ggagtagcca
    23761 tagtatggat tggacaaata ataatgattc aaccgcaccg ttacggacaa gaattgccct
    23821 gttaagagat tagtttgact ttccgcctct cctgccacag gagtgcctcc gtcgttcatc
    23881 tccgtagccg taatatccgc cttactagac tcagtcacga gttcaaagtt aaaagcacca
    23941 gtttgattcc agttctggat tgcttctaaa taagcctctt gaaagcgtga atccatctgc
    24001 ggatccaggt aaatacgagc agaggcctgt gcccaccttc cttcagaatg ctggtgacta
    24061 tctgtctggc ttaacttagg cagttgccct gttttctccc attggtcaat actttgttga
    24121 ccaattttta ctgaccgttc tgcttgcttt agcgctcctt gaaaatcccc gttcagatac
    24181 cagagaagtc cgaaagcaag cacacataag agcacaacta tccaaaccag acgccaaaac
    24241 aaacgccaca caaaagaaaa gaaagcccct atcaaacgaa aaagccagcg catgtctctc
    24301 cacctttcta gcaaataaga attattttac taaaagaagc taaaagaaat ctaaatactg
    24361 actttttcat cttaacgtcc tactattttt ttgagtaact ggatagcttt gtattttaaa
    24421 ggcgatggat ggtaacggaa agtacccgct ttgcgtacaa tatagccatt aaaattctgt
    24481 ttaaaacgca aaacaccatc acttccatca aaaatccctt gaatgcctag gaagttgtat
    24541 ttaggtattc cacgttttat gctttccaac ataacatatt tttgaagcag ggcaggggca
    24601 tagaacttat taaactcagt gtaggaacca ctaaagagat aagtcgtttc ctgaggcata
    24661 taaacaaata aactcccagc taaaacaata tcttcttctc catatttttc aatcaagtct
    24721 cgcgcttctg cttttcgaac ttcaaacgtt tcaaattgac tagaatattc tctcagttga
    24781 ttttgttttt tctcagaatg aggattttta ctcaaatcaa gtcgcaactt gtccaagttt
    24841 tcttctagtt tactttgttc accttgcaat ttgctcatat agtccgaaaa atttaagctt
    24901 gctatgagaa actccgcttg ttctccaaaa gtatcataaa aatgctcata atattctaaa
    24961 cttttatcac tatattctct acgttcagag gtttcttttg ttatattctt aaaaatcgat
    25021 agttcttcac gttttaactt tttcaaccga atgccaaagg tttcagcctt tttcaccaag
    25081 ggtttaccct ttttgctaaa acttttaagc aaactctttt cagttaattc agttaaatct
    25141 ttatagtata accaatctgg ttctccacct gggtaacctg ttgttaagcc atcaaattga
    25201 taacctaaat cagtcaaacc ttgaataata ctttttttct cagcatctat tggattacct
    25261 tggctatcaa aagtttgata agtttcataa ggttttacaa gcaactctaa tacaccattt
    25321 tgcttggcat attcttttaa ctctgcataa aaaactggaa gagcatcttg ttgggtataa
    25381 atcggccccg aattgagttc catatgcaga ccacccagca tgggcaagct ataaaccaga
    25441 gctgcaactt gaatttctcc ttcttgtttc aaagcaagat aaacaattcg agcccctctt
    25501 ttttctagca aatcccccat ctggacagat tgcataaagg aacgagaaga aacctgatca
    25561 gaataagtct gaaactcttc tttcgtgagt gttgttagtg ccatatactt actttctatg
    25621 tttttttctt aatgttttac ggaaatcaag agcaagtctt aacagaggat agagaggatg
    25681 agtgggcatt gtaaattcac ccaagtattc ttcaatcgtt ggattaaatt tttccttaaa
    25741 atgataaagt ccaccattga gagagttttc aacaccacct aaattttgcc aaaccatacc
    25801 tcgctcaaag gcatagcgag ccgtttcata ccatgttaaa attggtgcat tgtaacgttt
    25861 aaaatcatca tccataccag catatatatt gacagaggta gtaccaaatt ccaaactcaa
    25921 agtagccgct aaaggaactc tcgcttgacc tacatctata tattcctgca agaaggtcaa
    25981 ttcctctaac aaacgttctt tttccttctt ctgcgcttct acttttgaaa ttcgagtcga
    26041 ctcagtaaag gtctcttcca aggctctatt ttttgctaac tgttcttcta attcttgcga
    26101 acgtttagaa acatccaagg tggccaaggt gatataggcc ttgtccttaa aattatctaa
    26161 caatttttta taataggctt cattcctcaa atgaatctct tttcgcttct cagttttttt
    26221 catcaactcc gaaaatgaat ctaatagttc cagtccacca tattgaatct caagcccttt
    26281 gtttcgtgct gttcgaatag cctgttttgt tgacttggaa agtttatctt cttcaaaatt
    26341 ttccttgtat attttcgcct gaatacgagg ttgaatggtg tctcccattt cctccgtttt
    26401 tcctgaccac cttactccca tttgttgcaa actatcaata atagccagat tttcaggaaa
    26461 ttctgtcttt tcctgattga ttaaactttg agataggcaa atacttgggt caaaagtcac
    26521 aaaaaccgct ctcttactgc gagcatagga cttaatagac tgaatgacaa aattcaagag
    26581 ttctttatcc ccataatcca atataggtcc tcttgggatg taaaacattt tatagcctag
    26641 cggaagagtt ctaatcaaaa tactagctgt cgccagtaat ttttcttccc tgtaaacacc
    26701 aaacttctca tgttgccaat tagacttaac ttcctcccaa gcactacttt gtaatacatt
    26761 ggctaattca tgttctttga caaactgatc atattctaat gtgggaatgc caatttgata
    26821 acggtacatt tcctactctc tttcctccag tattttatta ctattctact actttcgttt
    26881 gaaaagtcta gtgaaaacat attttatttt agttctactc cccctatgct gaagttcaaa
    26941 ctaagtcagt ctctcttcta atttgaaatc aattggtagg gttgttaaaa aaacttccaa
    27001 ttgtttttcc cagtagtacc actcgtgagt tccagcgcta tggctatagg tcacatcaaa
    27061 acctagtttt ttgagatttt tcactgcgag attattggct tcgtacaaga aatcctgttc
    27121 gccacaccac gcccaaagtt tggtcttttt atccgatttt ttagccagac tttcaagaga
    27181 atagggacta gttgtccagt ctttaatctc tccaaaaaca cctctccagt aggctggact
    27241 tcccagattt tgactttcag gagaaaagtt ttgaaaactg agggcacctg aaaagctagc
    27301 tgcatgagaa aaacgatttg tcgcaagagc cagtttgaag cagccgtagc ctcccataga
    27361 aagaccagcg ataaaggtct tttcacgctt gctagtcata ttagggaaga agcgtttcag
    27421 aacctgtggc aattcctctg ctagagccgt gtagtagtca aaaccatact gggtatcggt
    27481 gtaccaacca ttgctggtat tgggcataac aacgatgaga ttagttcctc gaagcaagcg
    27541 ttctacattg gtccgcttaa gccaactatt atgatttcca gacatcccgt gcaaaaggta
    27601 caagacggga atatcttcac actctggttc ttccactcga ttggcatcag ggtagaggac
    27661 attcaccccc cactccatat ccaatacttg tgagtaatac tcgattttca ttactgccat
    27721 gattttctcc ttctattttt ctataagaaa aagccgaaac tcgactttct ctctgtttat
    27781 ttcaacgatt attttgcttc catgactact ggtaaaatag ctggacgacg cttggtttgg
    27841 tcaaagagat acttggtcaa attatctcga accttccctt taagatctgc ccagtcaaag
    27901 tcatctcctt gaagatactc ttctaccgtt tggttaatca attctgaact ttcatggaga
    27961 atatcgcgac tcttcttgag ataaacaaat ccgcgcgtat gaacacgagc cctagccaca
    28021 attttcttct cacgacggtt gactgtaata gccacgatga aaattccatc ctctgacaag
    28081 accttacggt cacgaagaac aacatttcca acatcaccaa tggcattccc atcaatcaag
    28141 atatctcctg ctgaaaccga tccagctgga acaaagtctc cattctcgta agccatggtc
    28201 gtcccctttt taggaatgaa gatgtgttct ggcaacatcc caactgccat ggcagcctta
    28261 gcgtgagcat ccaactcacg atactcccct tggacaggga agaggtactt aggttgcaaa
    28321 agattgatca tcagctgcaa atcacgcaca tttccgtgcc ctgatacatg taaactttgg
    28381 gtaatcaatt tgacaacccc acctgcctga taaatcatat tttccacacg cgcaacaaag
    28441 gcttctttag caatagacgg agccgtagca atatagacca ggtccccatc cttgatttct
    28501 acataacgat ggcgaccaat cgacatctta cgaagtccat tgataggttc acccatacga
    28561 cctgtctcaa gaataatcaa ctcatggtct tcaaagcgag acatatcttt aggcttaatc
    28621 aagagaattt cgttggctaa agacaacttc ttaagacgaa tcgctgtgcg gacgatattt
    28681 tcaatatcaa atcctgtcaa gacgatacgt cgacctgttt tatccgcagc gtcaaaaatc
    28741 tgctgaatac gagaaagatt actggaaaca gctgcaacga tgatacgacc ttcccagtca
    28801 gcaatagttt gggtaatttc atccctaact tcactttcac tagccacctg aatattgctg
    28861 tctgcattgg ccgaatcact gaggagagcc aggacgccgt cacgaccaat ctctgccaaa
    28921 cgagcaaaat cagttgcata agattcacta gccgtttggt caaatttgaa atcacctgta
    28981 taaacgatgc ttccttccga tgtcttcaag acaattccca gactctctgg aacggagtaa
    29041 gtcgtaggga agaaggaaac cactgtccca ccaaaatcaa tctccgtatt ctcatcaatg
    29101 acatggaaat cattaaattt cttaacggca tcatttcctt tgacaaagag ctttgccaac
    29161 tcaatggtca actcagaccc aaatacagga actttagcct ctgccaagag ataaggtaga
    29221 gcaccaatgg catccgcatg cccgtgggtc aagaaaaccc cagcaatacg gtcgctattt
    29281 tcaaaaaggt aatccatgtt tggaatcacc acatcgaccc ctaattgttc attttcagga
    29341 tattttaacc ctacattcaa aacaaaaatg gactctccaa tttcagcaat gtacatattt
    29401 tttccattct cacgcacacc accaagtgtt gttaaactga tattactcat ttttcctcca
    29461 aaatcttagt ttatcttgat tatagggcaa ctctaccctc ttattctaaa agttctaata
    29521 ctttcagccg aatcttttcc taccattata ccattttttt gatgattttc aaaatgaagg
    29581 tctgttttca aaaaagagtt gatttcccaa ctctttccta acttctactc tttttccatc
    29641 aaaaaatcat aaatttcttg caccgtaccc agcgccatca tttcttggtc tttaacggtt
    29701 tgtttgagat tttggtaaat ttgactttct cctatctccc gctttggcgc ctctttattg
    29761 actgtcatca caccatcttt tatcgtcaca aagcctagtt cttcaaatac ttgaatcatc
    29821 ttgaccagca agatttgttg aatattaaga tatgcagcca aatctttcag cttgtagcga
    29881 atatcaaact ctgggaactg gtaaatagtc ttgtacaatt tggcaaactg atctctagtc
    29941 ccataacctg tcagataata ggccttgtca atatcatttt tgaaatagac agcagagaaa
    30001 tgctgttcct gaaaaatggt cttcagctga gtaatatcct ctggaatgtt ttttacgaca
    30061 atagcttcac tagccgcaag atttggcagt tctccaggaa aatccaagac tggaacacct
    30121 tctggcaaga ctgcattttt tccacgaatg ttaaaaagtt gaacaccttc cactcgcgca
    30181 tccaccatca tcaactggag ggcagtttgg ccattccatt ggttgacaga caatttaacc
    30241 gctaactcta gattcttggt ttgagaaaac tctgtcgccc atctgccttg accaaaggct
    30301 accacttcaa aactcgcctc acccttggaa attttcagct ttagatgggc attacctgcc
    30361 cccatagtac gagcactttc gacctgaaaa ttcttgatat aaaaaatagg tttctgatta
    30421 tccattccaa aaggagctaa acgttcaaaa cttttgaccg tttccaagct aagtgcctcc
    30481 aaatccaact cttcatctag gtttaactta ttcttgccac cagcatctgc acctttttca
    30541 cgaacataat cttccaaaac ctgagataaa tctgagagtt gctcaacttc cagcgtcata
    30601 cccgctgcac ctgcatgacc tccaaaggcg atgaagaggt ctcgatgggg atccagagct
    30661 tcaaaaatat cgaccgcttc cacactacga gcactgccct tggcacgacc gtcttctata
    30721 ttaagaacaa tgactgtctg tcccaattct tccaataaac gaccagccac gattcctaga
    30781 accccaggat tccagccttc cttggccaag acctgaacct tcttctcagg atccaccatg
    30841 gtcttggctt cttcatagat agactgaacg atttccttgc gctcttcgtt tttctggtga
    30901 atcataaggg caatctcatg cgcttcctca tcatcaaatc cagtcaacaa atcaatggca
    30961 ggattgggat catccaagcg acccaaggca ttcaaacgag gagcaatctg gaaaccaacc
    31021 gtttcttctg ttacttcgtt ggcagcaatc ccagccatgt ccagcatttc ttgcagacca
    31081 atgcgctggg tatgacccaa catttccaga ccatattgaa ctaagatacg attttcatcc
    31141 gtcagactca ccatatctgc aatagttcca atagcgacca aatcaagcaa ttccacttgc
    31201 acttcttcta acagggcaca agccaacttg aaagcaactc cacaaccagc caaatattta
    31261 aaaggataat ccgcatctgg atgttcagga tggacaatag cataagcatc tggcagggtt
    31321 tcaggcatgg aatgatggtc tgtcacaatg acatctactc ccatagactg agccaatgca
    31381 atagcctcat gaccagcaac cccattgtcc accgtcacaa tcaaggaaat tccttcttgc
    31441 tcgataaagt atttataaac actagcatta gggccatagc catcggtaaa acgatttggc
    31501 aggtaaactc ggcactccgc accaagttgt tccaaacttt ccttcacaat agaagccgaa
    31561 gtcatgccat ccgcatcata gtctccataa acgagaatat tttccccttc ttcaatagcc
    31621 tgacgaatcc gctccactgc cttgtccata tcatggagca gataagcatc atgtaagtcc
    31681 tccaaggaag gttctaaaaa cttcttcaga ctttcttggt cctgaatccc tctctcaaac
    31741 aataaccgag ccacctcagg acccagtcca gccttcttgg ctatctttgt aaaatccgca
    31801 tcttctacct gcagggcaaa ctgccattca taagtaggtg ttatcaaaaa gacatccact
    31861 ctttctaaga attctattgc ttcattataa catgatttag acgttttctc tatccagatt
    31921 gcttttttat aacattttat agtatattga aactagaata gtacacctct acttataaaa
    31981 tattgttaga aatcgatttg actgtcccga tcgatttgtc cttttcttat ttcactatag
    32041 tgaatttttt cagatacggt ttgacaagaa cattctttta ttgtagaatt gtgttataaa
    32101 atctaacata aaggagatct ctcatggctt tagtagaatt taaaaacgtc gaaaaatatt
    32161 acggagacta ccacgcactc cgcaacatca atctccgttt tgaaaaagga caagttgttg
    32221 tcctgcttgg accttctggc tctgggaagt ccactcttat ccgtacgatc aatggtttag
    32281 aggctgttga caaaggaagt ctcctagtca atgggcacca agttgctggt gccagccaga
    32341 aagatttggt acctcttcgc aaggaagtcg gcatggtttt tcaacatttt aacctttatc
    32401 cacacaaaac ggtgttagaa aacgtgacac ttgcgcccat taaagttcta ggaattgata
    32461 aaaaagaagc tgaaaaaacc gcccaaaaat atctggaatt tgtaaatatg tgggacaaga
    32521 aagattccta tcccgccata ctatctggtg gacaaaaaca gcggatcgcc atcgctcgtg
    32581 gtcttgctat gcatccggaa ctcctcctct ttgatgaacc aacatctgct cttgatcctg
    32641 agactatcgg agatgttcta gcagttatgc agaaactggc gcatgatggg atgaacatga
    32701 tcatcgttac ccacgaaatg ggctttgctc gagaggttgc ggaccgcatt atctttatgg
    32761 ccgacggaga agttttatta gatacgacag atgtcgataa cttttttgac aatccaagca
    32821 aacctcgtgc ccaacaattc ctcagcaaaa ttatcaacca cgaaagtgac aaagtcaaat
    32881 aaggaggcgc ctatgaaaaa aaaattcttt ttatcagcat tattgattag ccttttcggc
    32941 cttgctgctg ccaaaccagt ccaggctgat actagtatcg cagacattca aaaaagaggc
    33001 gaactggttg tcggtgtcaa acaagacgtt cccaattttg gttacaaaga tcccaagacc
    33061 ggtacttatt ctggtatcga aaccgacttg gccaagatgg tagctgatga actcaaggtc
    33121 aagattcgct atgtgccggt tacagcacaa acccgcggcc cccttctaga caatgaacag
    33181 gtcgatatgg atatcgcgac ctttaccatc acggacgaac gcaaaaaact ctacaacttt
    33241 accagtccct actacacaga cgcttctgga tttttggtca ataaatctgc caaaatcaaa
    33301 aagattgagg acctaaacgg caaaaccatc ggagtcgccc aaggttctat cacccaacgc
    33361 ctgattactg aactgggtaa aaagaaaggt ctgaagttta aattcgtcga acttggttcc
    33421 tacccagaat tgattacttc cctgcacgct catcgtatcg atgccttttc cgttgaccac
    33481 tctattctat ctggctacac tagtaaacgg acagcactac tagatgatag tttcaagcca
    33541 tctgactaca gtattgttac caagaaatca aatacagagc tcaacgacta tcttgataac
    33601 ttggttacta aatggagcaa ggatggtagt ttgcagaaac tttatgaccg ttacaagctc
    33661 aaaccatcta gtcataccgc agattaagga ggatacccta tgacagattt atcatcttgg
    33721 acagcctatt ttcaggattt tggacaattt ttcaatggtt tcctctttac tcttgcccta
    33781 gcgattggat cctttatcct cgccatggtc ttaggaatct tctttggtgc catgtcaacc
    33841 agtaaacgtc caattttacg cattttagct cgcatcttcg tggaatttta ccaaaacact
    33901 cccctcttgg tgcagtttgt tattgttttt tatggcctac ctcttatcag tgaccacatc
    33961 atcatgattc caatttattg gacagccgtt ctctgcgtgg gactctatca cggcgcttat
    34021 atcgctgagg ttattcgttc agggattcag tctattccta gtggtcagat ggaggccgcc
    34081 ttgtcgcaag gttttaccta tatcagtgcc atgcgcttga ttatcttgcc tcaggccttc
    34141 cgcatcattc tccctccatt gaccaaccaa attgttaacc tcatcaagaa cacatctact
    34201 gtagctatca tctctggagt agacttgatg tttgtgacta agtcttggtc ggctctcaac
    34261 ggaaactata tcccagcctt tttaggtgct gctcttctct actttgccct atgcttccct
    34321 gttgcccagt ttggtcgcaa gatggagcaa gccaataaaa aagcctattc actttaggag
    34381 gttactatgg aatctatttt agaaatttta accccagata acctagtctt tatctttaaa
    34441 ggatttggct tgaccctcta tatttctctg attgccatca tcctctctac tatcatcggt
    34501 accgtgcttg ccgtcatgcg aaatggaaaa aatcctatct tacgcattat ttccagtatt
    34561 tatatcgagt ttgtgcgcaa cgttcccaac cttctctgga tttttactat ctttttggtg
    34621 ttcaaaatga aatccacacc agcaggtatt acagccttta ctctctttac atcagcagcc
    34681 ttggctgaga ttattcgagg cggtctcaat gccgtagaca agggacaata cgaagcagga
    34741 atgtcacaag gcttcacctc agcccaaatc ctctactaca tcattctccc acaagccatc
    34801 cgcaaaatgc taccagccat catttctcag tttgttaccg tgattaagga taccagtctc
    34861 ctctactctg ttatcgccct acaagaactc tttggagcca gccaaattct catgggccgt
    34921 tatttcgaac cagagcaggt cttcagtctt tacatcctga ttgccctcat ctacttcagc
    34981 tttaacctag cgatttctag cctgtctcat atgctagcca aacgttggca acaagctgca
    35041 gaataatact cttcgaaaat ctcttcaaac cacgtcagct tcaccttgcc gtactcaagt
    35101 acagcctgcg gctagcttcc tagtttgctc tttgattttc attgagtata aacagccact
    35161 ctccagttca attggagagt tttttgatga gagtagatat ttatagacct caatttgaca
    35221 ccatacattt tctatgataa aatgtaacaa attcatttac aacaaatcga aatctaaatg
    35281 aaatggaatt ttctatgaaa tcagtcaaag gactactctt tatcatagct agttttatct
    35341 tgactctttt gacttggatg aacacttctc cccaattcat gattccagga ctagctttaa
    35401 caagcctatc tctgactttt atcctagcca ctcgtctccc actactagaa agctggtttc
    35461 acggtttgga gaaggtctac accgtccaca aattcacagc ctttctctcc atcatcctac
    35521 taatctttca taactttagt atgggcggtt tgtggggctc tcgcttagct gctcagtttg
    35581 gcaatcttgc catctatatc tttgccagca tcatccttgt cgcctattta ggcaaataca
    35641 tccaatacga agcttggcga tggattcacc gcctggttta cctagcctat attttaggac
    35701 tctttcacat ctacatgata atgggcaatc gtctccttac atttaatctt ctaagttttc
    35761 ttgttggtag ctatgccctt ttaggcttac tagctggttt ttatatcatt tttctatatc
    35821 aaaagatttc cttcccctat ctaggaaaaa ttacccatct caaacgctta aatcacgata
    35881 ctagagaaat tcaaatccat cttagcagac ctttcaacta tcaatcagga caatttgcct
    35941 ttctaaagat tttccaagaa ggctttgaaa gtgctccgca tcccttttct atctcaggag
    36001 gtcatggtca aactctttac tttactgtta aaaattcagg cgaccatacc aataatatct
    36061 atgataatct tcaagccggc agcaaagtaa ccctagacag agcttacgga cacatgatca
    36121 tagaagaagg acgagaaaat caggtttgga ttgctggagg tattgggatc acccccttca
    36181 tctcttacat ccgtgaacat cctattttag ataaacaggt tcacttctac tataacttcc
    36241 gtggagatga aaatgcagtc tacctagatt tactccgtaa ctatgctcag aaaaatccta
    36301 attttgaact ccatctaatc gacagtacga aagacggcta tcttaatttt gaacaaaaaa
    36361 agtgcccgaa catgcaaccg tctatatgtg tggtcctatt tctatgatga aggcacttgc
    36421 caaacagatt aagaaacaaa atccaaaaac agagcttatt tacgaaggat tcaagttcaa
    36481 ataagtaatc atactctcct gggatttccc aggagagttt tttatagcca aatgaaataa
    36541 gacctgaacg atggaatttt ggcattcaaa tcaattgata agaatatttt atagtggagt
    36601 gaaataggat acgaacaaat tgattaggaa aatcaaatga atttatagaa atcttttagc
    36661 agttatgttg tcctattcta gtttcgaaac gctatagaag cagcattgtg ctagtctaga
    36721 ttcagtttac tatactaaaa cgagtagctt ggaatcaaaa aacccaccct cacaggcagg
    36781 ttttatctgt attattcagc tagattatgc tttaccttct gaaccgaata cgtcgatacg
    36841 ttcttcaacc gatgcttgga tagcttttac accgtcagcc aagaatttac gtgggtcgaa
    36901 gagttttttc ttgtcgtatt ctgcttcgtt tgcttcgtag tcacgagcaa atttacgagt
    36961 tgcgttagcg aatgcgattt ggcattctgt gttaacgtta actttggcaa caccaagttt
    37021 gatagctgct tggatttgct catcaggaat acctgatcca ccgtgcaata cgattgggaa
    37081 tcctggaaga gcttctgtca atttttgcaa gtggtcaagg tcaagacctt cccagtttac
    37141 tgggtaaggg ccgtggatgt taccgatacc agctgccaag aagtcgatac cagtttcaac
    37201 cattgcttta gcgtcttcga ttggagccaa ttcaccttta ccgatgattc cgtcttcttc
    37261 accaccgata gtaccaactt cagcttctac tgagatacct ttagcgtgtg ctttttcaac
    37321 aacttcttta gccaatttaa ggttttcttc aactggaagg tgtgaaccgt caaacatgat
    37381 tgaagtataa ccaacttcga tacactcaag tgcatcttcg tagtgaccgt ggtcaaggtg
    37441 gatagctact ggtacagtga tacccattga ttcaacaagg ttagcgatca agttgcgagc
    37501 aactttgtaa ccacccatgt atttagcagc acccattgaa gtttggatca aaactggagc
    37561 ttttttagct tctgctgcgc gcaagatagc ttgagtccac tcaaggttgt ttgtgttaaa
    37621 tccaccaact gcataaccgt tgtcacgggc tgcttggaca aatttttctg ctgaaacgat
    37681 tgccatttta tcaggcctcc tgtatatttt tatgggtcat cccatttaca ttgttcattt
    37741 tatcactttt tgccaaaaaa atctagtttt tcccgcagtt tcgattgatt ttcttctaac
    37801 tccatctatg taaacccttt ctctccctag tcttggacga cttttggaaa atctataaag
    37861 aaggttaaac gattctcctc catctcgaaa cgataagcta atttttcatg ttctaataga
    37921 ctcttaacca caaagagccc cataccagac cccttgacct tgcgactggc attgtcagaa
    37981 aaagactggg ctagtttttc ttgttcctct gagctacagc tattttcgat aaaaagttct
    38041 ccttctcttt ctccaattcg aactaagcca cctggaacag agtgcttaat ggcattgctg
    38101 atgagattag aaagaatcaa cttcataact gatgggttta gataagcctg ctgatgggtc
    38161 aaactattgt ctatctggag ctctctttcc ttggctagca aggcataatc tttgaccaga
    38221 ttttgcgtca tctggaggag gtcaattgtt tccctatcat ctcgcaattc ctgcacagaa
    38281 gagagggaaa gtatctgcag aacatggtga ttgagttcat ccacaatccc caaggcaact
    38341 cccagatact ggtctctatt cttataacga ccgatattct ctctcatatt ttcgattagg
    38401 attttcaaac tagccagcgg tgttttcaat tcatgagaag ctcctcgtag gaattcgacc
    38461 ttcatcttct ccagctggag aatggcttca ttcttttcat gcaagtccgc aataacagtc
    38521 aagagatgct ggtagaggct attgatttgt tccttgagat tgcctatctc atccttagaa
    38581 tccacgcgca atcgcacttg ggaatccagg tccatcatcc gacgggtcac ccgcttgatt
    38641 tccaaaatcg gtgcaacaat agtccgagcg tagatgtagg ccaccaagag ggaaatcaga
    38701 aaggaggcca gcaaggtata gggaagaaac tggagactga tttgctccgc ttccttttgt
    38761 aaatccatgg aagctagaaa ctggagaatc atagtaccac cgtcttgcgt tttcacctcg
    38821 cgctcctcaa taaagagaga ggttgtctgg cggtctgtgt ccagaggaag actgtccttg
    38881 acttctaact tgtcctcggt catctcacct ttgacggtcc ccttgatatc actagtctgg
    38941 gaatacaagt ctaacacttg ctcgatactc tgcctatctt tcccttctag ggactgggca
    39001 atggctgttg ccttttgacc aatggtttcc tgacgatgac tcagataagt cgaaggaaaa
    39061 agaaaataaa tagctaaatg aaggcagata accagaacac taaatatcga gaaggtatag
    39121 ataaatatct ttgtaaataa acctgttcgt ttcattttcg ctccaattta taaccaacat
    39181 tgcgcacagt gaggatacaa tccaagtcta gctttttccg caattccttg atataaacat
    39241 caataacacg gtcaaaggga acctcatctg tcgctttcca gacggcatcg ataatctgag
    39301 accgagtcaa ggcccggcct tcatttttca ctagatagtc cagaatttcc aactctttgg
    39361 cattgatagg cacttcttga cctgcgaggc ttgcactgta gctttcaaag tccaccttgg
    39421 tatccttgta agaaaagatt cgtcctgtat cgtagtagcg cttgaaaatc gcgtccaccc
    39481 tcacttttaa gagggagagg gagaaaggtt tttccagata gccatctgcc aaagaggcaa
    39541 aggcactcat cttgtattcc tcatcctgaa aagctgtcaa catcaagaca ggaacctgac
    39601 tggttttacg aatctcagct aggacttcta agccgttaag cttgggcatc tggatatcca
    39661 gtaaaaccag ggccacctca tagctagaaa attgctccag agcttcctga ccgtccgctg
    39721 cctcaatagt ttcatagcca caatccgtca aataatcact gaccccctca cggatcatct
    39781 cttcatcttc tacaattaaa attttcatac tttaactgct ctctattttt tatttttctt
    39841 agaataaata cctactctat tttctattat agtctcttgc tggccttttg tctgtaagca
    39901 actgaccact agataaaacg ttgtgaaatt cctttctcat aaattccata actttagtat
    39961 attatattta agcactaaag tacaaagaaa gcaactgaaa gcaatgattt tcaccactgc
    40021 tttcagattt attttgaatt gttaaatagc tattcctatc cactattctt gaatagaaac
    40081 acaagatgca atctttattc cagactcatt tttttcaaat ttattcacca tccagcaaga
    40141 gctcttttgg ttgttttcta aggagattgc ttgaagcaag cgccataacg agaaccacta
    40201 gaaccaaggc aaggacaaaa atgatgataa agtctgatgt ctgaatggaa atgtctaggc
    40261 tcgacaaggt cttgctaaag ccatctactt ctgcaccgcc accaaggtta gaggcttgag
    40321 ccgccttact agcctgtttg gcaacacctg aagtcacatt ggcaaggaca gtgtttccaa
    40381 ttgcacgggc agtgtaatta gctaggaagt aagcagaaac tagagcaggg atagcaatca
    40441 agatagattc ggtgatgaat tgacccaaga tacttgcctg cttgagacca atagagagga
    40501 ggattcccac ttccttgcga cgggcattga tccaaagact gagcaagagg gcaaggagaa
    40561 ggactgagaa gctcaagcta ccccagaaga ggaggttggc catcttgtac ataccagaga
    40621 tagattgctc aagagctggg tagttagagg agctcttgac gagtgtgtag ctcttccagt
    40681 tgataccact gatgccattc aactctttca taacatcatc caagttcttg tctgctgtta
    40741 caaagaaggt tgcgtcccca taaatggctg tgtcttctgt gtatccataa agttttgcag
    40801 cagtgtgaat gtctgtaata gctgtgtttt cgtaaagttc ttgtgagtag gttactgctg
    40861 acttattatg accatcaaag agtcccttga ttgtcacttc aactgtttcc ttggctcctt
    40921 tttcattatc tgcatcgtag atattagagt ccagtttaac cttgtcccct actttccagc
    40981 cgtgtttggc tgccaagtcc ttgtgcaaga ggattttatc cttgtcgtcg ttggttaagt
    41041 gctctccttc gactagttta taagaaccag agacaaactt atcttcttta gaggagtcat
    41101 tgacacctgt aatcatcaag ctacttccaa aacgtttggc acgatcagca gtgagattct
    41161 tcttggtttc tggcgtttca atcaggtcat atccagtcaa atctccgata gcgttgatac
    41221 gtttgacata agactcaatg gccttgtttt cggtgatttt tttgatgtct tcacccttga
    41281 tattcccagc accacgaggc gttccttggt tgacgcgacg attgatttgc atggagaagc
    41341 tattggtgat atttttaaag gtctcctgag aagccttggc agtagctccc ttgattgaca
    41401 agccgaccaa actcaagctc gccataagga gaataatcag gaagatgaca atcgatttga
    41461 aaaacttcct tgtaacatag gcaaatgcgt tgtgtaacat agattccctt tctagatttt
    41521 gttttaatca ttctatcaaa ataagctcaa attatttact agtattgcgc gtttcagtca
    41581 gtttcttatc ctttaattca agtgtaatat ctgacgcttg tgccacttct ttactgtgag
    41641 ttacgacaat cacacattta cctgttttct gggcaagtga tttgagtagt tcgacaatat
    41701 ctccagcagt tttaggatcc agatttcctg ttggctcatc agctagaata actggagctt
    41761 ctgagaccaa actgcgagca atggcaacac gttgctgttg accacctgat aactggagaa
    41821 cattccgctt aatctggctt tcatccaaac caagctcaag aagtgtattc ttgcttgcct
    41881 ttttgttgac caattggata ttttccagcg gagaaagata atctatcaag ttataatttt
    41941 gaaagaccag ggaaatatgg tgcatgcgat ggtaagaata gcccttctta cgaatatcct
    42001 ctccttgaaa aaggatagaa ccttcaacag gactatctag accagcaagt agggacaaga
    42061 gtgtggattt tcctgctcct gactccccaa taatactgta aaattttccg ggttcaaaat
    42121 tataattgat ctgatatagg actgcttcag cagtattctt ataacggtag gtaacatctt
    42181 gtaattgtaa taaagtcatg atttctcctt cttaactaat agatgataaa atttctttcg
    42241 gtgattttct aaataagaat aggaaacaaa gggctacaga taagcaacta agcagaacta
    42301 gaaaaacata ggattctgca aaagataaga tgctagttga taaactgctt gctttggcta
    42361 gtgtatcttg taagcttacc tgatctccac ttgctagtag agtttggagt aggtaagttg
    42421 tgattccgtt tcctgcaaca aatgctggaa gcaaagctcc aagagatacc aaaactacct
    42481 ctaaacagaa ttgtaggaag atcgagctct tgccttttcc aagtgcaagt aaaatcccca
    42541 cttcatagac ccgttctctc aaccagagag acaaaaccag aattaaggct ccagctcctg
    42601 ctatcaacat cccataaagg aagatggtca ggaaggtttg gaaagttgca actgagtctt
    42661 tgatttgttc aaaagccttg ttttcctttt cgacttggta gccttgattt tccaaggcca
    42721 agttttctac ctgcttcatg agtccgtcca tttccttagg attttctaca tagaagcgtg
    42781 ctgcactgac ttgagcttca ctattgccca aaagggtttg gctactttca taatctgtaa
    42841 agacttgatt ttcactgaag tcagaagaca agcctgtgaa tttctcttgt tttttaccag
    42901 aaaagatgcc gataatctca aactctactg tttgtccttt tccagattca gactgaccag
    42961 catccaagcc aatcttgtca tgaagtgaaa gaccgttctt cttagccaat tcttcgtgga
    43021 taaggatttt cttggaatcc cctttttgaa ggtgtcgccc ttcttttaga ttgaaagccg
    43081 aactggtaaa ggttacatcc ttggatgaat cctcaagagc cgttaagcta accaagttat
    43141 tgtctgcagc tgataaatca tcacgctcca cgctctgctc gccagtcact gcttccttgt
    43201 cttttagttt tgcgaccgtc tcaagttcag gagagacatt ttccagcccc ttaatcttgc
    43261 ttacagatgc taggtctgac aacttgaatg tctgaccatt ctctatcttc ttaatagaaa
    43321 aagatgtatt gagtgattta taaagattgc tttctactgt tttgttggac ttcatcagag
    43381 tcaaacaggc tgaaattccg gccaataaga ccaataaaat cagaaataaa ataaaacttc
    43441 tcagtcgctt tctgctgaca taagcccaag atctttggat tggattcatt tgtcacctcc
    43501 atatttgtaa gactattata aaacccaaat atgaaatgtt tatgaaatac gaaaaaaaat
    43561 atcgagtagg ggataatctc tagcccctct cacaccaccg tacgtg
//
LOCUS       DAVBNO010000014        43432 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVBNO010000014 DAVBNO010000000
VERSION     DAVBNO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.014"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(15..1535)
                     /locus_tag="VQ846_000804"
     CDS             complement(15..1535)
                     /locus_tag="VQ846_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET3075421.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(1528..2256)
                     /locus_tag="VQ846_000805"
     CDS             complement(1528..2256)
                     /locus_tag="VQ846_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075422.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(2271..3035)
                     /locus_tag="VQ846_000806"
     CDS             complement(2271..3035)
                     /locus_tag="VQ846_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET3075423.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(3039..3482)
                     /locus_tag="VQ846_000807"
     CDS             complement(3039..3482)
                     /locus_tag="VQ846_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET3075424.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(4164..4460)
                     /locus_tag="VQ846_000808"
     CDS             complement(4164..4460)
                     /locus_tag="VQ846_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET3075425.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(4482..5546)
                     /gene="pepA"
                     /locus_tag="VQ846_000809"
     CDS             complement(4482..5546)
                     /gene="pepA"
                     /locus_tag="VQ846_000809"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET3075426.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKDEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            5948..6649
                     /gene="galE"
                     /locus_tag="VQ846_000810"
     CDS             5948..6649
                     /gene="galE"
                     /locus_tag="VQ846_000810"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET3075427.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            7072..8031
                     /locus_tag="VQ846_000811"
     CDS             7072..8031
                     /locus_tag="VQ846_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3075428.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSEGLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            8021..8977
                     /locus_tag="VQ846_000812"
     CDS             8021..8977
                     /locus_tag="VQ846_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET3075429.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            8974..9726
                     /locus_tag="VQ846_000813"
     CDS             8974..9726
                     /locus_tag="VQ846_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET3075430.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINNKKICIYS"
     gene            9822..10787
                     /locus_tag="VQ846_000814"
     CDS             9822..10787
                     /locus_tag="VQ846_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET3075431.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTTFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(11012..11254)
                     /gene="yidD"
                     /locus_tag="VQ846_000815"
     CDS             complement(11012..11254)
                     /gene="yidD"
                     /locus_tag="VQ846_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET3075432.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLSRILRCHPWSKTGKDPVPDRFSLKRNQEGE"
     gene            complement(11254..11976)
                     /locus_tag="VQ846_000816"
     CDS             complement(11254..11976)
                     /locus_tag="VQ846_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET3075433.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLIIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(11963..12532)
                     /locus_tag="VQ846_000817"
     CDS             complement(11963..12532)
                     /locus_tag="VQ846_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET3075434.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(12551..13279)
                     /locus_tag="VQ846_000818"
     CDS             complement(12551..13279)
                     /locus_tag="VQ846_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET3075435.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(13279..14019)
                     /gene="xerD"
                     /locus_tag="VQ846_000819"
     CDS             complement(13279..14019)
                     /gene="xerD"
                     /locus_tag="VQ846_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET3075436.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGGKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(14010..14471)
                     /locus_tag="VQ846_000820"
     CDS             complement(14010..14471)
                     /locus_tag="VQ846_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET3075437.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDTEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(14468..14989)
                     /locus_tag="VQ846_000821"
     CDS             complement(14468..14989)
                     /locus_tag="VQ846_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET3075438.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(14965..15936)
                     /locus_tag="VQ846_000822"
     CDS             complement(14965..15936)
                     /locus_tag="VQ846_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET3075439.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYQTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(15933..16727)
                     /gene="racE"
                     /locus_tag="VQ846_000823"
     CDS             complement(15933..16727)
                     /gene="racE"
                     /locus_tag="VQ846_000823"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET3075440.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(16934..17182)
                     /locus_tag="VQ846_000824"
     CDS             complement(16934..17182)
                     /locus_tag="VQ846_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET3075441.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(17324..18949)
                     /gene="treC"
                     /locus_tag="VQ846_000825"
     CDS             complement(17324..18949)
                     /gene="treC"
                     /locus_tag="VQ846_000825"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET3075442.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(19127..21094)
                     /gene="treP"
                     /locus_tag="VQ846_000826"
     CDS             complement(19127..21094)
                     /gene="treP"
                     /locus_tag="VQ846_000826"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET3075443.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            21279..21989
                     /gene="treR"
                     /locus_tag="VQ846_000827"
     CDS             21279..21989
                     /gene="treR"
                     /locus_tag="VQ846_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET3075444.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(22022..22825)
                     /locus_tag="VQ846_000828"
     CDS             complement(22022..22825)
                     /locus_tag="VQ846_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET3075445.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFIYVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQYPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(22838..23715)
                     /locus_tag="VQ846_000829"
                     /pseudo
     CDS             complement(22838..23715)
                     /locus_tag="VQ846_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(23727..24665)
                     /locus_tag="VQ846_000830"
                     /pseudo
     CDS             complement(23727..24665)
                     /locus_tag="VQ846_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(25156..26082)
                     /locus_tag="VQ846_000831"
     CDS             complement(25156..26082)
                     /locus_tag="VQ846_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075446.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQSIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(26093..27160)
                     /locus_tag="VQ846_000832"
     CDS             complement(26093..27160)
                     /locus_tag="VQ846_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075447.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            complement(27169..28095)
                     /locus_tag="VQ846_000833"
     CDS             complement(27169..28095)
                     /locus_tag="VQ846_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3075448.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            complement(28095..29591)
                     /locus_tag="VQ846_000834"
     CDS             complement(28095..29591)
                     /locus_tag="VQ846_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3075449.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            complement(29658..31637)
                     /locus_tag="VQ846_000835"
     CDS             complement(29658..31637)
                     /locus_tag="VQ846_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET3075450.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            complement(32323..33516)
                     /locus_tag="VQ846_000836"
     CDS             complement(32323..33516)
                     /locus_tag="VQ846_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET3075451.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(33654..35096)
                     /gene="gtfA"
                     /locus_tag="VQ846_000837"
     CDS             complement(33654..35096)
                     /gene="gtfA"
                     /locus_tag="VQ846_000837"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET3075452.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKHLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            complement(35373..35453)
                     /locus_tag="VQ846_000838"
                     /pseudo
     CDS             complement(35373..35453)
                     /locus_tag="VQ846_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(35454..36287)
                     /locus_tag="VQ846_000839"
     CDS             complement(35454..36287)
                     /locus_tag="VQ846_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET3075453.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(36301..37167)
                     /locus_tag="VQ846_000840"
     CDS             complement(36301..37167)
                     /locus_tag="VQ846_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET3075454.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIIAIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFFQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(37181..38440)
                     /locus_tag="VQ846_000841"
     CDS             complement(37181..38440)
                     /locus_tag="VQ846_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET3075455.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(38532..40694)
                     /locus_tag="VQ846_000842"
     CDS             complement(38532..40694)
                     /locus_tag="VQ846_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET3075456.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLFYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            40802..41662
                     /locus_tag="VQ846_000843"
     CDS             40802..41662
                     /locus_tag="VQ846_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET3075457.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            41659..42594
                     /locus_tag="VQ846_000844"
     CDS             41659..42594
                     /locus_tag="VQ846_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET3075458.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(42720..42807)
                     /locus_tag="VQ846_000845"
     tRNA            complement(42720..42807)
                     /locus_tag="VQ846_000845"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42771..42773),aa:Ser,seq:gct)
     gene            complement(42814..42887)
                     /locus_tag="VQ846_000846"
     tRNA            complement(42814..42887)
                     /locus_tag="VQ846_000846"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42851..42853),aa:Ile,seq:gat)
     gene            complement(42923..42993)
                     /locus_tag="VQ846_000847"
     tRNA            complement(42923..42993)
                     /locus_tag="VQ846_000847"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42959..42961),aa:Gly,seq:tcc)
     gene            complement(43013..43085)
                     /locus_tag="VQ846_000848"
     tRNA            complement(43013..43085)
                     /locus_tag="VQ846_000848"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43050..43052),aa:Phe,seq:gaa)
     gene            complement(43089..43162)
                     /locus_tag="VQ846_000849"
     tRNA            complement(43089..43162)
                     /locus_tag="VQ846_000849"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43126..43128),aa:Met,seq:cat)
     gene            complement(43173..43262)
                     /locus_tag="VQ846_000850"
     tRNA            complement(43173..43262)
                     /locus_tag="VQ846_000850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43224..43226),aa:Ser,seq:tga)
     gene            complement(43273..43346)
                     /locus_tag="VQ846_000851"
     tRNA            complement(43273..43346)
                     /locus_tag="VQ846_000851"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43310..43312),aa:Ile,seq:cat)
     gene            complement(43360..43432)
                     /locus_tag="VQ846_000852"
     tRNA            complement(43360..43432)
                     /locus_tag="VQ846_000852"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43397..43399),aa:Met,seq:cat)
BASE COUNT        13023 a         9795 c         8075 g        12539 t
ORIGIN      
        1 aagtctctgt cccatcattt cttcaacaaa ccttgttctt ggagaaactc cttggctact
       61 tgctttgctg acttgccttc aacaccgact tggtagttga gctggctcat ctggctttct
      121 gtaatcttac cagccaatgt attaagaact ctttccaact ctgggtgttt cttgagaaga
      181 gcttctttca tgagtggagc cccttgataa ggtgggaaga gttgcttgtc atcttccaag
      241 acctgtaaat cataacgctc caattccgca tcagtcgaat aggcatccgt gatttgaata
      301 tcccctgact gaattgcctg ataacgaaga gctggctgca tagtcgccac attgagattg
      361 agaccataca ttgattgcaa gcccttattt ccatcttcac ggtcgttaaa ctcaagcgta
      421 aagcctgcct tcagctgccc ttccactttt ttcaaatccg aaatggtctt caagccatat
      481 tcttgagcaa tctttttcgg aacagctaca gcataggtat tttgataaga cataggtttg
      541 agataggcta gatgatcctg cttggcaatg ccatcacgcg ccacttgata aacctgctct
      601 ggctcatgac tcaccttggg tgatggttga agcaaacttt cagtcaccgt accggtaaat
      661 tcaggataaa tgtcaatatc accttttttc agagcttcat aaaggaaact tgtcgtccca
      721 aaattcggtt taacagtcgc agtcatgctg gtattttctt caatcagcaa tttatacata
      781 ttagccaaaa tttctggttc tgggcctatt ttcccagcaa taatcaagtt ttccttctct
      841 ttttgagcca aaagggctgg actataagac agacccagta ataaagccac caaggcaaaa
      901 ccagagaaaa tcgtccgcaa tttcgctttt tccattactt ttagtaggaa gttaaaggca
      961 atagctagca ctgcagaaga aagggcccca atcaaaatca aactggcatt attacggtca
     1021 attcccaaca ggataaagga acccagtccc cctgccccaa tcaaggtcgc caaggtagcc
     1081 gtaccgataa tcaagaccgc tgccgtccga attccagaca tgataacagg catggcaagt
     1141 ggaatttcaa acttcttgag acgctcccat ctggtcatcc caaaggcaat cccagcctct
     1201 tgaagactcg gatcaattcc cttcagccca gtaatagtat tttgtaaaat cggaaaaatc
     1261 gcataaatca ctagagctgt caaagccggc aaggtcccaa ttcccatcaa agggataaag
     1321 agccccaaca aggccagaga cgggatggtc tggaaaatac ctgcaatctg caagacccag
     1381 tcggccagct tctcatgata gcgaagaaaa acagccaagg gaatcgcaag caaaatagct
     1441 agtaacaagg tcaaaagtga caactgcaaa tgttgagata gagctgtcaa ccaatcacta
     1501 aaacgatcct gaaaagttgc aattaaatta gtcatgaaca ctacctccaa acaagtctgc
     1561 tacaaagtct gttgcaggca cttttaaaat tgtctcggga ttcgctacct ggcgaatttc
     1621 tccatcctgc aagacagcaa tacggtccgc caacttcaag gcttcatccg tatcatgggt
     1681 tacaaaaatc gttgtcatcc caaactcttt atgcaattct tttgtcagaa cctgcaactg
     1741 ttttctcgaa atagcatcca aggccgaaaa gggttcatcc atgaggaaaa tcttgggctg
     1801 accaatcata gctcggacaa taccgacccg ttgctgttct ccaccagata attcactagg
     1861 taagcgatgc ccatactcgg ctactggtaa accaacctta gccaaaagct cttctgtttt
     1921 cttcgtaatt tcttccttgc tccacccctt catttcagga atgagagcaa tattttccgc
     1981 aactgttaga tttggaaaaa gagcaatagc ctgtaaaaca taaccagtag aaagacgaag
     2041 ttcacgctca tcatagtctt tgatgcgctt cccatccata taaatatttc catcagttgg
     2101 ttccaaaaga cggttaatca tcttgagcat ggtcgtctta cctgacccag aaggccctac
     2161 taaaaccata aattccccat cctcaatctg taagttgaca tctctcaaga catccttttc
     2221 tgtgtagcgc agtgctacat ttttgtattc aatcattctt tgtcctcaat ttaaaacttc
     2281 cctcgattag tcaagtcttc taccttaggc atgacttcct tattatccca atgctccaca
     2341 attttcccgt tctctaaacg gaagatatcg tactgggcat aagcaacgcc atcaatctga
     2401 gtctgaccat agctaaccac atagtttcct tgtcctaaga gttggaaaac aaagtcaaaa
     2461 gtgacactat attcagccac atagttttta taagcagcac ttccttgtcc aatctcatga
     2521 ttatgctgaa tcaaatcgtc tgccacataa tcactccact gctctagctc cccattttgg
     2581 aaaatttctg tcaagaaacg gcgaaccagt tttttatttt ctgctttctt gtccaaatcc
     2641 ttgatttcaa aatctccaaa aatttgatct agttgatcat tttcaggtgt tcgatagtag
     2701 tcaatgacat cccaatgctc aacaatacga ccattctcat ccgcacggaa agtatccgtc
     2761 gtcacccatt gagcttctcc accattcaga tattgatgaa catgaacaaa gaccagattg
     2821 ccatcctcaa tggtgcggac aatcttaatc tgacgctctg gatgacgctc aaagaaatct
     2881 gcaaagaagg ctgcaaatcc ttctttcccg tcaggaacac ctgtcgaatg ttggatatag
     2941 gtatccccta cagactgggc ttgagcctca gcaactcgtc cgtcttgaat ggcatggatg
     3001 tataggttgt gagcattttt cacttgttgt gacatattct aaacctcatt tcccttctct
     3061 ttcaaattcg ccaaaattct ttcttgaaaa ccttcaaatt ggtgaatttc ttcctctgaa
     3121 aatcctttgt aaaagatagt atccaatttc tgactgacac gatgccccac ttctttctgg
     3181 gacttgccta actccgttaa aactaaatac ttcttacgct tgtcttttcc acacggacta
     3241 acaattacaa gcttttgttc ctctagcttt tttatcatag tcgttagcgt attattcgca
     3301 agcccagtcg caagcgcgat atctgtcgca gttgcgcagc cagtttcact attccataaa
     3361 accgctaaaa tcttgccctg ttcaccccta taaagagcct caggatcttg actcagtaac
     3421 ttttgaaaaa tccgcccatt caacaaacga atatgatggg ctagcaaatg accatctttc
     3481 ataacacctc caatttattt cgatatcgaa atgaataaaa caattgtaac actcatcgtt
     3541 ctaactgtca actatttcga tttagaaata atttttgata attatccaca ccaccatact
     3601 ccggctcaac taacttttaa cgagagtttc taaactcctt cgtcctccag tctacaaaag
     3661 ccttccattc gtactatcct atattttatg aggggacaca tttttcctat cagaccattt
     3721 attttaaaga tagaagtaaa tcataattgc ttccatctgt tcttttatag tatattgaag
     3781 ttagactaga gcactgtatc ttctaaaaca ttgatagaaa gcgatttgaa tttcccaatc
     3841 aatttgttcg tatttatagc atttcgaaac tggaatagga caccatgact gctaaaagat
     3901 ttctataaat tcatttaatt tcctcaatca atttgttcat atcttatttc attccgctat
     3961 aatttcacct taccctatct ttttcgtagc acccttcaaa cagcctatcc cctaccgttt
     4021 gacgattcct cacttcgctc cacttccatt acagaagttt cttcactact atgggctcgg
     4081 ctgacttctc atgattcctt gttactacta tttgaacgct cacgagatag atcttacaaa
     4141 aaatgctttg atccacaatg gaatcaaagc attttaaaga gttcctcata cataagcgca
     4201 gaagtcgcag ttcctctgta cttggcttct tctcttttga taaagcgagc caagttgagc
     4261 aactcaggtg ctggatgttt gggatttagg agcaattcac gattgaccag gcctgagaga
     4321 cgaactgcct gcaattgctc atttgtagta ggcagttttt tagcagtctc taggagagca
     4381 gcaactaaat cttcactcaa atcatgtcga gcatgattgt aaagatcttt tataaggctt
     4441 tctaagtttg gttctgccat ccctaccacc tcccttatgg tttaataatg tttaatcaaa
     4501 tcaaccgttg aacgatccaa tttcttcacc aaggcttgta agaaagcttg cgcttctagg
     4561 aagtcatcca ttgcatagag ggtttggtga gaatggatat aacgagcgca gacaccgata
     4621 gttgttgatg ggacaccacc atttttcaga tgagctgcac ctgcatctgt tccgccttta
     4681 ccacagtagt attggtactt gataccagct tcttcagccg ttgtcaaaag aaaatccttc
     4741 atccctggga gaagcaagtg acctggatca tagaaacgaa tcaaggttcc atctccaatc
     4801 ttgccttgac caccgtagac atcacctgct ggtgagcaat caactgcgag gaagacttct
     4861 gggtcaaact tggttgtaga ggtatgagcg ccacgcagac caacttcttc ttggacgtta
     4921 gaacccagat agagttcatt gccgagtttt tgacctgata aagcttcagc tagctcgctt
     4981 accatgagga caccgtagcg gttatcccaa gcttttgaga tgatattttt ttcattggct
     5041 gtcaaaattg cagaactatc tggtacgatg gtatcaccag gacggatgcc aaaactttct
     5101 gcctcatcct tgtccgcaaa accaccatca aaaacgatat cggcaatggc tggcatggtt
     5161 ggtcccccct ttccacgagt caaatgcgga ggaacagaac ctgaaatcac aggaatttca
     5221 tgaccatcac gagtcaagag tttgaaacgt tggctgctaa ctaccatggg gttccagcca
     5281 ccgatttcta cgacacggaa ggtaccatct ggcttgattt cgctgaccat aaaaccaact
     5341 tcgtccatat gagaagcgac caagacgcgc ggtgcatcca cagcttctga atgtttgata
     5401 ccaaaaatac cacccaagcc atctgtcacc acttcatcca catgcggtgt caacttttca
     5461 cgaagataag cacggacagg cgcttcatga cctgagactg cagcaagttc tgttacttct
     5521 ttaatttttg aaaataatgt tgtcatttca gttccttctt tctttcatcc attttaccac
     5581 tttttatagg agaaggatag tgggaaggtg gatttctaag ttagtatctt agtcctgctc
     5641 tatcttagaa aaggatagta ttctcttgca tgtagtgcaa aatctagtaa acattccaaa
     5701 attaactcga atatttattt ccaaacaaaa aaaacaatac accatcaaag ttgtttggat
     5761 ttttcatgaa atttacagaa aatagttgac ttccctttct tctttcttta aatatatagt
     5821 tggttgagtt tggaatagta cgctgtagct gctgaaacat ttctagaaat taattcgact
     5881 ttcctaatag agttgttcat atcttatttc aatttactat agtacaaaac tagaaaagga
     5941 aaaaatcatg accaaaacaa ttcttgttac aggtgggact agctacattg gctcccatac
     6001 cgttaaagct cttttaaatg ctggctatca ggtgcatgtc ctcgataatc tctccacagg
     6061 aaatcgagcg gctgtggata gtcgtgctag ctttaaagaa ctggatgttt atgatgctag
     6121 cgccttaaag gcttacttag aagaaaatca gattgatgct gtcctccatt gtgcaggtga
     6181 aattgttgtg agcgaaagta ttgaaaatcc aagtaaatac ttcactgcca acgttgctgg
     6241 tatgaaccaa gttctcaaag tcttatctga agttggcatt caaaaaatca tgttctcttc
     6301 gactgcttct ctctatggaa ataactgtat tgacaagccg gtgactgaag ataccctgct
     6361 cgaccctgtc aacccttatg cagagacaaa actgatgggc gaacgaatga tttactggat
     6421 ggccaatcgc tacgactgga aatatgttat tttccgttac tttaatgttg ctggggctga
     6481 aatggatgct tcaaacggtc tgcgtgtgaa aaatccaact cacatcattc caaatatcaa
     6541 caagaccgca ttgggacaac atgatagctt aaaaatattt ggagatgact acgatacacg
     6601 tgacggttca tgtattcgag attacattta tgtcttggac cttgcatagg ctcatgttaa
     6661 aggaatgaac tacctatttc aagaagacag ttcttctcaa atctttaact taggaactaa
     6721 aaaaggctat actatcaaag aaatctttaa aactgctgaa gaattactaa attaaaaaat
     6781 cccacacgag attgttgctc gacgtgctag tgacccagcc agtgtcctag cagatgcttc
     6841 aaaagctaaa caatatcttg attggacggc tagctactcc ctcgaagata ttattttatc
     6901 agattaaggt aaaaacattt tagaatagga taacaaagga gaaagtcttg ttgctctctc
     6961 cttgttgtat ttattaaatt gtcagaaatt ttattgacag attagaaaaa taatgttaca
     7021 tttatatccg cagatatctt tcgataccaa atctacatga aaggacgggg tatgaaactt
     7081 tctcattatt taattggctt acttctactc ctagtctttc tctctattag cattgggacc
     7141 agtgattttt catggggaaa gctatttgat ttcgaccagc agacctggct cctctttcaa
     7201 gagtcccgtc tcccaagaac tatcagtatt ctcctgactg cctctagtat gagtatggca
     7261 ggccttctca tgcagactat tacccaaaat cagtttgctg caccgagtac agttggaacg
     7321 actgaagccg ccaaactggg aatggtgctg agcctttttg tctttccatc ggctagtctg
     7381 acccaaaaga tgctcttcgc ttttgtttca tccatcgtat tcaccctctt cttcctagcc
     7441 tttatgacca tttttactgt aaaggaaagg tggatgttgc ctctgattgg gatcatctat
     7501 agcgggatta tcggctcagt cacagaagtt atcgcctatc gtttcaatct ggttcagagt
     7561 atgactgcct ggacccaggg ctccttctcc atgattcaga cccatcagta tgagtggctc
     7621 ttcttaggcc tcatcatcct gataaccgtt tggaaattat cccaaacctt caccatcatg
     7681 aatctgggga aagaaaccag cgagggtttg gggatttcct actccctact tgaaaaactg
     7741 gccctctttc tggtggcgct aacgacaagc gtcaccatga ttaccgtggg tggcttacca
     7801 tttctcggag ttatcgttcc caatcttgtt cgcaagcgct atggagataa tctaagtcaa
     7861 accaaactca tggtcgcact ggttggtgcc aatctagttc tggcttgcga tatcctatcc
     7921 cgagttctga ttaggcccta tgagttgtct gtcagtctct tgctaggaat catcggtagt
     7981 ctcgtcttta tcctacttct ctggagaggg ggacgaaaag atgcagacta aaagcaaaca
     8041 taccaagctc ttctggattc tcattattct tgccatcgga gcttgtcttc tctacttttg
     8101 gcccatcact cacttgtcag cctttgcttg gaagttgcgt tcccaaaaga tcatcgttta
     8161 tctcttggta gccatcgcga ctgggatttc gaccattagt tttcaaaccc tgacggaaaa
     8221 tcgcttcctg acgcctagta ttttgggaat tgaatccttc tacgtcctac tacaaaccct
     8281 actactggtt tttgaaagca agtttcttca actgggcaaa tcccctatct tagaattcct
     8341 agtcttactt cttgtccagt ccctcttctt tctcgcctta caaggttact tgaagacact
     8401 gatgaagcaa gacctggtct tcatcctgct gatctgtcta gcgctcagaa gtctctttcg
     8461 aaatatcagc accttccttc aagtcctaat ggatccaaac gaatacgata aactgcaaaa
     8521 tagtcttttt gcctcctttc aacatcttaa cacttccatc ctagccatcg gttctctgat
     8581 catcctcgct ttgacaatct ttttctttcg aaaagcagtc gttctagatg tcttgcacct
     8641 gcaaagagaa acggctcaga tattgggact cgatgttgaa aaagaacaga aagagctcct
     8701 ctggggaatc gtgcttttga cctcaacggc cactgccttg gtaggaccta tggccttctt
     8761 cggctttatg ctggccaacc tcacctacct gattgtcaaa gactatcagc acaagttact
     8821 ctttatagtg gccattctgg ttggatttat tagcttaacc ttggggcaag ccttgattga
     8881 acgagtcttt gcactggaaa ttcgtatcag tatgatcatt gagagtgtgg gtggcttctt
     8941 attctttatc ttactatata ggagggctcg tcagtgaaac tggaaaacat tgacaaatcc
     9001 attcaaaaac aggatatttt gcaaggcatt tcgcttgaag tcagtcctca aaaactgaca
     9061 gcctttattg gtccaaatgg tgctggaaaa tcgactctcc tctccatcat gagcagacta
     9121 accaagaaag atcagggagt tctcagtatc aaaggacgtg aaatcgagag ctggaattcg
     9181 caagaactgg cccaagaact aaccatccta aaacagaaaa tcaattacca agccaaattg
     9241 actgttgaag aactggtcag ttttggacgt tttccctaca gccgaggtcg acttagatca
     9301 gaagactggg aaaaaatccg agaaactctg aactatttgg aactgaccaa cttaaaagac
     9361 cgctacatca atagcctgtc aggggggcaa ctccagcgcg tctttatcgc tatggtactg
     9421 gcccaggata cggactttat cttgctggac gaaccactca acaatctcga tatcaagcaa
     9481 agcgtcagca tgatgcagat tcttcgacga ctggtggagg aactcggcaa gaccattatc
     9541 atcgtcctcc acgatatcaa catggccagt cagtatgcag atgaaattgt cgccttcaag
     9601 gacggccagg tctttagcaa gggaagaacc gatcaaatca tgcaggctga cctgctcagt
     9661 caactttatg agattcccat cacgctagct gatatcaata acaaaaagat ctgtatctat
     9721 agctagtaac ataaaagctc aagttagaga accttcagtc tcttagtcaa taagattaag
     9781 agactcccta aatcgttatc acattttaaa aaggagaaat tatgaaaaca tcccttaaac
     9841 tttatttcac tgccctagtg gccagcttct tgctcctact tggtgcatgt agtacaaact
     9901 caagcactag tcagacagag accagtagct ctgctccaac agaggtaacc attaaaagtt
     9961 cactggacga ggtcaaactt tccaaagttc ctgaaaagat tgtgaccttt gacctcggcg
    10021 ctgcggatac tattcgcgct ttaggatttg aaaaaaatat cgtcggaatg cctacaaaaa
    10081 ctgttccgac ttatctaaaa gacctagtgg gaactgtcaa aaatgttggt tctatgaaag
    10141 aacctgattt agaagctatc gccgcccttg agcctgattt gattatcgct tcaccacgta
    10201 cacaaaaatt cgtagacaaa ttcaaagaaa tcgccccaac cgttctcttc caagcaagca
    10261 aggacgacta ctggacttct accaaggcta atatcgaatc cttagcaagt gccttcggcg
    10321 aaactggtac acagaaagcc aaggaagaat tggccaagct agacaagagc atccaagaag
    10381 tcgctactaa aaatgaaagc tctgacaaaa aagcccttgc gatcctcctt aatgaaggaa
    10441 aaatggcagc ctttggtgcc aaatctcgtt tctctttctt gtaccaaacc ttgaaattca
    10501 aaccaactga tacaacattt gaagactcac gccacggaca agaagtcagc tttgaaagtg
    10561 tcaaagaaat caaccctgac atcctctttg tcatcaaccg tacccttgcc atcggtgggg
    10621 acaactctag caacgacggt gtcctagaaa atgcccttat cgctgaaaca cctgctgcta
    10681 aaaatggtaa gattatccaa ctaacaccag acctctggta tctaagcgga ggcggacttg
    10741 aatcaacaaa actcatgatt gaagacatac aaaaagcttt gaaataagcc tcttcaaact
    10801 caatttaata ctcaatgaaa atcaaagagc aaactagaaa actagccgca ggttgctcaa
    10861 aacactgttt tgaggttgca gatgaaagct gacgcggttt gaagagattt tcgaagagta
    10921 taaaattttg acattctaaa acgcatccta tcaagttcac tcaaacctga taggatgcgt
    10981 ttttatcatt ttgaaatcta tttaccccac ctcattcccc ttcttgattt cgtttaaggg
    11041 aaaagcggtc tggaacgggg tccttacctg ttttcgacca gggatgacaa cgtaaaatcc
    11101 gagacaagcc catcaatacc cccttaaaac catgtttttc aatagcctga atcatgtagt
    11161 tggaacaagt cagctcaaag cgacaagagg gtggaaagac tggtgagata aaacgttggt
    11221 aaaagcgcac aggcgctatt aaaattcgtt tcattatttc ttagttacag ccatggtgtg
    11281 tagttggctg atttcttttt tattaagacg acgggattct cctggacgga gtcctgtcaa
    11341 gtctaggtgt ccaaaacgag tccgagacaa cttatctact tggagaccaa cagcttcaaa
    11401 catcttttta acctgatggt tacgcccttc atggatgatc aactgcacca cagagcgatt
    11461 tttgactggg tccactttga gaatttcata aacagctggc ttggttttct taccatcaat
    11521 ctcaagacca cgggtcaagg ggcggagatt gtccttattg gccacacctt taacacgcgc
    11581 gacataaacc ttgtcaatct cattacgagg gtgaatcatc tcgtctgtaa agtccccatc
    11641 attggtcaaa atcaagactc ctgatgtatc ccagtccaaa cgacccacag ggtaaatacg
    11701 ctctttgaca ttgggcaaga ggtcgacaac cgtcttgcga cccttatcat ctgtcacact
    11761 ggaaatcaca ccgcgtggtt tgttaagcag atagtagacc ttttcttcgt tgtagatagg
    11821 ttgaccttca acttcgacct tgtcgcctga cttgatagtg gttgctagtt cacgcaccac
    11881 ttggccgtta accgtcacca agccttgctt aatcagctct tctgcttttc tcctactggc
    11941 cacacctgcg tgggcaatat acttattgat tctcatcttc ttctatcctt tcaccaaata
    12001 attggctttc ttgggcttga atctcaagct catcaatcac tggtaattct tctaaatggt
    12061 ttatccccat gtaatctagg aaataatccg tagtcacata gaggttgggg cgccccaata
    12121 cttccttttt cccgtcttcc tttatcaggt caaaagcctg caactttgcc aaggctccac
    12181 tcgagttaac tccacggatg gcatcaattt ctatccgcgt aatcggctgt ttgtaggcaa
    12241 taatggacaa ggtctcaagg gcagcccgag acaagctctg gttgataggc gccttagagt
    12301 attccttcaa aatctctgca aattgaggct tggtcaccaa tctataagca ccacttgtct
    12361 caatcaaagc caaactggaa tctgggtcct tttcatactt ctgggctaat tttcctaaac
    12421 tttgctggat gcctgtcggt ggtagagaga ggagttcagc taactggcgg acccgaatcc
    12481 catcttcacc cgctacaaac aagagcgctt ctatttttgc taaagtactc atctttcctc
    12541 tctatcaagt ctagctttga ggcacttgac tttcttcctt cttttccatg agatagatat
    12601 ctccaaaact ctcctcttgc acgaggatca gctcctgggt tttgattaac tctagggttg
    12661 ccaaaaagag ggtgatgacc tcttggacat tctgggcttc cttgaacaaa tcctgcaagc
    12721 gcaattgatc tcgtccaatc aaggactctt tcacgataat catcatgtcc tcaatcttat
    12781 actcatcccg caagatcgtc gtgtgatttt gtgcaaactc ctcttttttc ttggctagga
    12841 tatttgaaaa agccaaaaag aggtcaatgg tcgtcttgtc atgcacaagc tccgcatctt
    12901 cgtaaatcaa ctctgtcggc gctttggaat aatactgggc ccgttcttgg tgcttggctt
    12961 ccaagtgctc acccaagagc ttgaacttgc gatattcttc gatttgagag aggaggtcct
    13021 gctccaggtc atcccccaag tctgtcactt ctgctacctt cggaaggagt ttacgactct
    13081 taatcagcat gagctgacta gccatgacca tgtactcacc cgtcacttcc agacgcatgg
    13141 cctgcagggt tgagacatag gctagatact gttcgatgac ttccgtaatg ggcacatcgt
    13201 agatatccat ctggtactta gaaaccagat gcaagagtaa gtccaggggt ccttcaaaat
    13261 cttttaattt aatatccatt atctatattt ttctaaggtc aggactgttt ttaatcctaa
    13321 tttttttgca atttcgtaca aatcgacctt gttttctatt tgtcttagaa taaactgttc
    13381 acgtaagact tgagctgata aggatggaaa acctttctcc ttgacaaaag attctaactg
    13441 acgaaaggcc cactgacgag aatagggttt ccctcctctt tcaaaaagat aggtctgccc
    13501 catcaagggt tccaattctg aaagcaaggc cgtgggaatg gtgacaatcc tctgttggga
    13561 agccttgctg attcgcagca cctgaaaatc cagattgatg tccgcaacct tgatggctaa
    13621 aatctcactg ggcaagagcc ccatttctag gattaagagc gctagcaagc ggccctctgg
    13681 atggtcgctt tcctgccaaa aagagtctag gtatagaatc tctggctttt ccgtcttctt
    13741 ttcagcttgt ttggctaatt ccaagcggta aaagctgtcc acctctcctt tttgatagag
    13801 aaagtataga aattggttac aggccgaaat ctttcgcttc tgggcgctga tttttagatt
    13861 ggctagctgg gcttggtaaa tcttgagact ggtctcagaa atccgctcac ctaccatgtc
    13921 taaaaattgc tccaaatcat acttatagga ctgcttggaa ttgacagaca agccctgctt
    13981 ttcctctaaa aaggctgaaa ttctgtctct cattggcatc gaatctcata ttccttacta
    14041 aaatcatgca agagggcatt aacggccttg aggatggact tgcgcgtaat aatcccttgg
    14101 aaaataccct ctgtatccac aaccggtaag aaggactcat ctactagctt gtgcaagacc
    14161 tccgtaatgg tgaaatcagg cgaaacaacc gctacgtccg tttttgtcat atgaacgata
    14221 tccgtatccg ccatgatttc ttggctcaag tcatgctcca tctgataagc cataatatct
    14281 ctgagtccaa tcgtcccaac aaactgtttt tcatctgtca caacgggaac acgggtatag
    14341 gtcatctgac taagcaagag ggtcgcatga tccgcattgt gggtatcaat caacacagct
    14401 agatttttag caggggtcaa aaaagtttcc tcctgcccca acaagaaagt ctcaaactcc
    14461 ttggcaatca tcggctaaac tccttggaca aacctggata cacctcgtga tctcgtgtca
    14521 aaaagtccac tttgaagtaa ctatcatcaa tctccacacg agcatagaga cattctctga
    14581 tggtacctcg tggttggctg atagaacctg gatttagaaa gaggatcttg ccttccatcc
    14641 aagcacttgg cacatgcaag tgaccataga ggcagatagc ggcctcttcc tcctgagccc
    14701 agtagtccaa cttttgaaag ttgaaattga tgtcaaacaa gtgaccatga gtttggataa
    14761 tcttggtcga accaagctca gtcaccagac gttctgggta gccggcgtag aagtccatgt
    14821 tccctttaac aacgcggatg ccctcccaaa gtggagaatc cggacgtagt tcagaatcgc
    14881 cgttatgaaa aacggcatcg actttgccca catagcgatc acggacttct tccacaatca
    14941 agctatcgcc atgggaatcg ctcattacaa tgatggtttg ctttgccatg atggaaatac
    15001 ctccaaaagt ttcttaacgg ctaaggcacg gtgagattga ctattttttt cttccagggt
    15061 taattcagct gatgactcac ctgtctctcc tacaaggaag aggggatcat agccaaagcc
    15121 attttcaccc ttaggttcaa agttaatata acctgaccag tctgcttcaa caactaaact
    15181 ttccttattt gggctggcta cgactagggt tgtgtggaac tgagccgagc ggtccttgag
    15241 ttcaaagacc atggccaatt cgtgcaagag tttggcatta ttttcacggt cagttgctcc
    15301 cacacctgcg aaacgagctg accagacgcc tggtaagcca ccaaggacat cgactttgag
    15361 accagaatca tctgccaaaa ccatcttacc cgttaattga gaaatggttt ctgccttaag
    15421 gcgggcattt tcttcaaagg tcatacctgt ttctgctact tcaggcaggt cagggtagtc
    15481 attaagattt tccacatcgt agcctaactt atcaaagata gctcggaatt ccttggtttt
    15541 gccttcgtta cgagtcgcaa tcaacaaggt ttctctgacc ttgcttgtct caaagaaatc
    15601 atgaacattg accccttctt taggcaattc tacatacaag agttgcccat tttcaaccaa
    15661 gagcagggcc ccatgacgtt gaataacttc caagtttcgt cccatttctt gattaagcat
    15721 atctaccaca aaggagaaca aacggtaggt agaaccatag cgtaaaacag taactgaaag
    15781 cgggaaacca tactcttcat ctccaaatat tttggagaat tcaaacagag gaaaatctgt
    15841 ctgatagtcg ctcaaactgt taacgccacc aaaaatacta taagacccaa cataccagtc
    15901 ctggtcatcc ttatattcat aaattttatt tgtcataatt ctacatgctc cacatgaatc
    15961 tctttttcca gccattcttc accaatttgt gcaaaacttt ggctactggc tgttgtgtaa
    16021 aaacggtgat ggagtggtcc agcatcgcga ccacgattga tttcaaaata attgagtaag
    16081 actgagatat cccgtacgca ctctgcccca ctatcgatga gctgaacctt tggccccatc
    16141 acattttgga taatagggcg aaggagtgga taatgagtac agcccaaaat caggctatcc
    16201 acctttccaa ccaagggacg cagggtttca tagaccacct tcttggtaac actggttgac
    16261 agggcacctg actcaaccaa gggagcaaac ttgggacagg ccaagctctc cacctgtaag
    16321 tcgggatcca gatcatggat tttctgacgg tatatgtctg attgtaccgt catgggcgtt
    16381 ccaatcactc cgattttccc accttgactg gacttgatgg ctgccgaagc tcctggcaaa
    16441 attacaccca agacaggaat atctagttga gccttgattt cttcccagac gaccgcagtc
    16501 gcagtgttac aagcaatgac aatcattttg acatccttgg tcaagagaaa gttgaccagc
    16561 tgccaagtat attcacgaat ttgctcagca ggacgggggc catagggcgc ccgcgccgaa
    16621 tctccaatat agacgatttc ttcatgggga agctggcgca tgagctcgcg cacaacggtc
    16681 aagcccccga cacccgaatc caaaaaacca attggtcgat tatccataca gtcttctttc
    16741 tagtcttttt tctgattgat cgttcattat gattccttgt actgaacgaa ctgaccgaca
    16801 gcttgataga atgtcaacta cctctctctt aatcataatc aaatatcaat agaatgcaag
    16861 gaaaaagtct catccctaaa ccataaccaa catagtgaga agtctggaac tttcatccca
    16921 gacttttcct tatttatttt ttctttttat tgttagcaag ggctgccttt tgttggcgga
    16981 tgatttggtg gtaaacttgt tgtaccttag cttcgcttgg tttttgacca tttgcactca
    17041 aaagagtacg aactgcttca gcattcaaac gtgggttgtc agcaaattct ttttcgattt
    17101 gcttacgaac caagtacatt cctccaagag ctcctcctag aaaagctagc acaatcaata
    17161 caattgctaa aagtaaatcc atcatttttc tcctgtttct ttttgagtcc ctttcattat
    17221 accacaaact agccaccctg accagtttgt tctacacttt caaggatgga atagacagca
    17281 aaaagaaccc tgcttttctg aaaaagaggt ctccttgcta agtttaattt acataaatag
    17341 ccagtgtttg atagggtttt agtagaattt tctcagacac ttctgcatct tcatagtttg
    17401 aaatcaaaat ctgtccattt tggtagactg ctggcaagtc gatttccact tctttagcat
    17461 aaaagttatt gagcactagt aacttttgat cctcaaactg gcgttcaaaa gcgtagactt
    17521 gtttgctatc ttcaaaggct ggtttgtaac ttccttctga aatgattggc atttccttac
    17581 gcagtcgaat caagtcttga tagaaggtaa aaatcggacc ctggatttca ttttctacat
    17641 tgatgtattt ataggattta ccagctttca accaaggagt gcctgttgaa aatcctgcat
    17701 tttccgaagc atcccactgc atgggaatgc gtgaattatc acgcgactta gcttgaataa
    17761 tctggaaggc ttcttgctga ctctttcctt cttctaagag catctgatag gcattaagcg
    17821 attcgacatc cacataatca gccatagaat catagtctgg gtcaatcatc ccgatttcct
    17881 cacccatgta gatataaggt gtcccacgtg acaggtgaat gctggctgct agcatggtgg
    17941 ctccttcctt gcggaagttt tgaatatcga caaaacggtt caaggcacgt ggttgatcgt
    18001 gattgttcca aaagagggca ctccaaccgt ctttatcact catttcctta ccccaactat
    18061 ggtaaagact cttcaactct tcaaaatcaa agggagccaa ggtccacttt tgtccatcct
    18121 tatagtccac cttgaggtga tgaaaattaa aggtcatgga taattcctga cgatcaggcg
    18181 acgaatagag gacacagttt tccatggtgg tagaagacat ttccccaact gtcataaagc
    18241 tatcgtcaga tccaaaagtg gcttggttca tcatacgcaa atagttatga acgatgggtt
    18301 tgtctgtata agctggcttc ccttcatttt caggacagtc cactgaaacc tcgtccttac
    18361 caatcaaatt gatcacatca aatcgaaaac ctttgacacc cttgtcgcgc cagaaattaa
    18421 caaccttgaa aagctcctta cggacattgg gattgcgcca gttaaggtca gcctgggtct
    18481 catcaaatag gtgaagatag tatttcccag tatccccgaa aggcgcccat gcagaaccac
    18541 caaacttaga ctgccaatct gttggttggt cttggatgaa gaaaaagtct tgataatact
    18601 tatcaccagc tagggctttc tgaaaccatt catgctctgt cgaacaatga ttaagtacca
    18661 tgtccagcat aaagtcaatc ttgtgctctt taccgataca caccatttcc tcaaaatcag
    18721 ccatatcacc aaaaagagga tccactgcca tataatctga aatatcgtaa ccattatccc
    18781 gttgaggact tggatagaat ggattgagcc agaccatatc cacacctagt ttggctagat
    18841 agggaatttt tccgataatc ccacggaaat ccccaatacc gttttcagtg gtgtctttgt
    18901 aagattttgg atagatttga tagactactt ttcctttatc aagtgtcatc tgtttctcct
    18961 tttctgataa aagggaggaa gcagtcttcc gtccctattt gtgctatttc aattatactc
    19021 aatgaaaatc aaagaacaaa ctaggaagct agccacaggt tgctcaaaac actattttga
    19081 ggttgcagat agagctgacg tggtttgaag agattttcga agagtattag attcgtgtag
    19141 cgaccatgag agatgctcca gcttggatcg ttgtcggata agttccggga atagtcgctg
    19201 tataagcatc ttggttggtg atgataacag gagtttctgt caccagacct gcagccttaa
    19261 tgacatccat atcaaaacga atcagttgct gaccaactgt aacgtgatct ccttggacta
    19321 caagactttc aaaacctttg ccatcaagac ctactgtatc cataccgatg tggatgagca
    19381 attcaactcc ctcgtcagag acaatgccga tggcatgctt ggtagggaaa agaaccgtca
    19441 ctgtcccatt aactggagag gtcaactcac cttggcttgg ttcaatgact agaccttgcc
    19501 ccatgacacc tgatgcaaaa ataggatccg tcgcttgact caattctttc acttggccag
    19561 ttagtgggct gataatttct accgaagtaa gttctactgg ttcatggttc acaaattctg
    19621 cttcttcttg agcaacgaat tctgcctgca agttcgtatc gccctctgtt tttgtaaaga
    19681 gaccagcctt gcggaagaag aaagtcaaga gcattggaac aacaatcgca actagcatag
    19741 ttcctgcaaa tggcagcatg tattgaggtt gaatagagag aatacctggc aaaccaccga
    19801 taccaataga agccgcagtt acattaaaag taacggataa catgcctgca agggctgaac
    19861 cagtcatccc agcaacaaat ggataaatat attttacgtt aaccccaaaa agagctggtt
    19921 ctgtaacacc gagataggct gaaatggttg caggaagtga aacctgagcc tcacgctcat
    19981 catggcgatg catgaaataa taggcaaaca cggctgagcc ttgagcaata ttagaaagag
    20041 caatcattgg ccatagggca gtgccaccag catccgcaat caattgtgta tcaatggcat
    20101 tggtcatatg gtgcagacct gtgatgacaa atggagcgta gagggcacca aaaattgcac
    20161 cgaagagcca tttaactgga ccagttaaac ctgccaagac aactgatgaa agcccttgtc
    20221 caattgtcca accgattggt cccaaaacag tatgagccaa aatcaaggct ggaatcaatg
    20281 acaagaaagg tacaaaaatc atagaaatga cttctgggat atgcttgcgc cagaagattt
    20341 caagataaga cagactcaaa cctgcaagca aggctgggat aacttgggct tggtaaccga
    20401 tacgattaac agtaaaatag ccaaaattcc aaacccagtt tgccgcgata tcagctgctg
    20461 gcgttgaagc aaccgcatag gcattgagca actgaggcga taccaaacag attccgagaa
    20521 caattcccaa aatttggctg gttcccatct tacgagaaac agaccaagta atccctactg
    20581 gtaagaactg gaagatagct tcaccaggca accagaggaa gtgattgaca cctgcccaaa
    20641 actgagagga ttctgtgatg gtcttgccat ccaacatcga ccaatggaca ccttccaaga
    20701 cattacggaa accgaggatc aatcctccga ctatcaaggc tggaataatc ggagtaaaaa
    20761 tctccgccag agtggtcata acacgttgga ccacgttttg attactctta gctgcagact
    20821 tggctgcttc tttggaaaca ccctcaatac ctgaaacggc tgtaaaatca ttataaaaga
    20881 tgggcacgtc atttccaatg attacctgaa attgacctgc atttgtaaaa gttcctttaa
    20941 cagctggaat tgactcgata gctttaacat tagccttctt atcatctcct aaaacaaagc
    21001 gcatccgtgt cgcacagtga gttacggcag tcacattttc tttgcctcca attgcctgaa
    21061 gcagatcttt ggcttcttgt tcaaattttc ccatttgatt ctccttatac ttgtcaaagc
    21121 aagtttgatt tgataagtat attgtaaacg ataccaaact tgtctgcaac ttgtttggta
    21181 taaaaagggt gtttttttgt aaattttcta gcacttgtat ggtaaaatag atacaggtgt
    21241 tcattaaact agactaaaaa cctatttaag caggcaaaat gaagaaatac caacaattat
    21301 ttaagcaaat ccaagaaacc attcaaaacg agacttacgc tgtcggagat ttccttccta
    21361 gcgagcacga ccttatggag caatatcaag tgagtcgtga taccgtccga aaggccctgt
    21421 ctctcctcca agaggaagga ttgatcaaaa agataagagg gcaaggttct caagtcgtca
    21481 aagaagaaac cgtcaatttc cctgtatcca acctaaccag ctaccaagaa ctagttaaag
    21541 aacttggact gcgctctaaa accaacgtgg tcagtctgga caagattatt attgataaaa
    21601 aatcctcact gataaccggt ttcccagagt ttcggatggt ttggaaggtg gtccgccagc
    21661 gtgtggtgga tgatctggta tccgttctgg atacagacta tctggatatg gaactaattc
    21721 caaatctcac tcgccaaatt gctgagcagt ctatctattc ttatatagaa aatggcctca
    21781 aactccttat tgattatgct cagaaggaaa tcaccattga ccactcaagc gaccgagaca
    21841 agattctcat ggacattggc aaagaccctt atgtcgtttc gattaaatca aaagtctatc
    21901 tccaagacgg acgccaattt cagtttaccg aaagtcgcca taagttagaa aaatttagat
    21961 ttgtagattt tgcaaaacgc aagaaataaa agactgagac accagatctc agcctttttc
    22021 tttatcccaa aactaattca tcactgacca gactttggcc actatttttc tggaagagat
    22081 gcataaggtc ttcaactgtc agatgctttt tctcttctcc cttgacatcc accactatct
    22141 tgccttgata gagcataatg agacgattgc cgtactcaat cgcatgattc atatcatggg
    22201 taatcatcaa agtcgtccac tgatggtgct ccacaatctt ttgcgtcaag tccatcacca
    22261 tttgactggt tttcgggtca agtgccgcag tatgttcatc caaaagtaaa agtttgggtt
    22321 tcaccagagc tgccatgact agggtcaaag cctgtctttg tcctcctgag agatattgag
    22381 tatctacttt taagcggttt tcaagaccaa tattcaactc cttcaaggcc tcttggaact
    22441 ggattctatc cttctccttc acaccccaac caagccctct cttttgccca cgtctcaagg
    22501 caatagccat attctcctca atcgtcaaac gagaagctgt tcccatctta ggatattgaa
    22561 aaacacgggc aatatccttg gctctctttc tgacactcag atttttaatg gatttccctg
    22621 ccagtagaag gtccccttcg tccaccgata gattgccagc caagatattc atcaaagtgg
    22681 attttcctgc tccatttcca ccaatcacat agataaaatc tccctcttca acctctaaat
    22741 ctaatccttt gaggacatgg ttctcattga ccgtccctgc ttcaaatgtc ttgtgaatat
    22801 tttcaagtgt taacaaactt gccataactt tctcctccta ttcatttctc aatttcagac
    22861 cacgaatctt taatctcttt tgcaattctg gtgcaaataa aaccaaagcg agcaagattg
    22921 cattaaagag acgtaccaag ttttgatcta gatttggaat ttcatagata tttaaaataa
    22981 tcaaacggta aacaatagca ccgattccga tggataataa gcggcctcca atggtcaagt
    23041 cgtgtatcaa gacttccgag ctcaaaccaa caacaatggt tcctgtccca gaagtcacat
    23101 ctgaaaatcc atcattttgg gcaaacaagg aaccacatag ggcaatcaaa ccgtttgaaa
    23161 tcatgtaacc aacaatcttc atggtgtcta cattgactcc attggcctca cttatcggga
    23221 tattatcccc agtcgaacgc aagaccaaac caatctctgt tttcatcaaa agggtcaaga
    23281 ccaaacaagt aagagacaga ctaaactaag tgagaaaaca gctccttcat ttgtcagacc
    23341 caagctcgcc aactgtttaa agacggttgc agaatctccc aaggaaagat tgggtacact
    23401 gcccataatt ttaatattga ttgaatagag ccctgtcaag gtcacaatcc ctgtcaagag
    23461 agctgggatt ttcatcttgg tgtggagcat tcctgataca agacctgcta ccatacctgc
    23521 cagcaaagca agtaaagtcg caagccaagg atttgcccct gcctgtatct gtgatacaac
    23581 gactgctgcc cccagaggaa aggctccctc agcagttata tccgcaatat ccaaaatacg
    23641 gaaagtcaag tagaccccaa tcgccacaat cgaccacaac aaaccttctg ataaactaga
    23701 taacacaaaa ttcatcttaa tcctccttat tccttgcctt ctaacttact aatatcaatt
    23761 cccaatgcat ctgccatttc ttgattggta tgcaattcca gtttttcagg caactcaact
    23821 gctatatttt ctggtttctc accttttaaa acacgaatca gcatgcgagc tgtctgtctt
    23881 cccaattctt cataattggt tccgtagtta tacaagtcac caacagcaat catttctgtt
    23941 gaaccaccga atactggaac cttgtgttta atagaaacct gcttaactgt ttccatggtt
    24001 gacgaaatga tattatcagt tgggacaaaa accatatcca cctctgccat caaactctct
    24061 gctgccgcct tgacgttatt actgtccaag attgtttttt caacaacggt aaagcctttt
    24121 tctttcagaa gtcgcttagc ttcatcattt taaacaaccg aattgggctc gctctgagta
    24181 tagaggattc caatcgtttt agcctttggc aacactttct ttatcaaatt gatttgggtt
    24241 gaaatggcat ctgatgactg atcgcttgtc ccagttacat tgcccccagg atgttctctt
    24301 gactcaacca acttggcact gactgggtct gttacagctg aaaagataac tggtggcgtt
    24361 tgcgtcgtat tggccaagct ctgagcagaa ggccttgcga tggctagaac gacatcgctg
    24421 gattctgcta gttgctggga aatcgttttt agatttcttt gttctccctg tgcattttgc
    24481 aaatcaatct caatattttc cccctcaaca tagccttgtt tggccagctc atccacaaat
    24541 ccttctctag tagcgtctaa ggactggtgg gtaataaact gcgaaatacc gatacgaaag
    24601 acatctcttt tcttatctgc cttcaactga tgcaaactga tcagagaagt caagaggatc
    24661 cccactacca agagggggct agtgattttc gaacaacttt cataatgaaa ctccttctca
    24721 gaaaagataa aacaaaaaac cgcctagata ctatctaaac gggtacttga aataacctaa
    24781 caagttacaa cttagctagt ccatttagat attcatctat atggaccaca atcaaaaatc
    24841 ttagccacat agatacaaac aaattgcttg aactgtttgc atccatggtg acatgtctac
    24901 aaacactatc taaagtagct aaagtaaaag ttttgattca tagttacggc ttgtctctta
    24961 ttcatttctt tttctgcttt cttttttgat ccttcctatc ctaccacctt tttcatcacc
    25021 tgtcaataat atttcaaaat tttttaaaat ttttcgaaaa ttctttcagg aagctttcaa
    25081 agtttttcaa atcagtccaa caaaaaaagt tttataattc ccataaaaat tgacatggaa
    25141 attataaaac cattattagt ttagtccttt ttgataacgt gccaattcgg cttggttcgc
    25201 ccaaacatag tgacctggac ggatttctac catagatggc ttatcagtct catagtcgtg
    25261 ttgacttggg tcgtaaacct tcaagacctt cttacgttcc aagattggat ctgggattgg
    25321 tactgctgaa agcaaggctt gagtatatgg gtgaattgga ttgttaaaca attcttctgt
    25381 ttctgcaacc tctacaataa cacccttgta aataactgcg atacgatctg aaataaagcg
    25441 aacaaccgac aagtcatgag cgatgaagag ataggtcaag ccgagctctt tttggaattt
    25501 tttgagcaag ttcaagactt gggcacgtac agaaacgtcc aaggctgaaa ttggctcatc
    25561 tgcaataaca aagtctggtt gcatgaccaa ggcacgggca ataccgatac gttgacgttg
    25621 accgcctgag aattcatgag ggtaacgagt caagtgctca gcaagaagac ctacttcacg
    25681 gataatactt tgaactttct ctttacgttc ttcttcatcc ttaaataaac ggtgattgta
    25741 aagaccttca gaaataatat aatcaacagt cgcacgttca ttcaaacttg cggcagggtc
    25801 ttggaaaatc atctggattc gacgaatcaa ttccgcagct tgttcacgcg atttcttacc
    25861 attaatcttt tgaccatcaa aaatgatatc tccattactt gtatcattta gaccgatgat
    25921 agcacgacca atagttgttt tcccactacc ggactcacct acaagcgaga aagtttctcc
    25981 cttgttgata aagaagttag catttttaac cgcgacaaac ttcttacttc cttcaccgaa
    26041 ggaaatttct aaatctttga tttctactaa tttttcagac atttccttcc tcctagtcag
    26101 ccagatgggc aaatcccatt ttttcacgga tcttatcatg gagatttgca atcacagctg
    26161 gtttttctac ttttggagca tcctcatgaa gaagccaagt tttagcccaa tgtgtctctg
    26221 atactgagaa ttgaggagct ttttgttcga agtcaatctg cattgcgtag tcagaacgca
    26281 aggcaaaagc atcccctttc aggtcagtat aaagtgacgg aggtgttcct gggattgagt
    26341 aaagatcccc tttatcatca gcaagctgag gcaagctaga caagagactc catgtatatg
    26401 gatggcgagg gtcatagaag acttcctcaa ccgttccata ctcaacgatt tctcctgcat
    26461 acataaccgc taccttatcc gcaatacttg ccaccacacc aaggtcgtgg gtaataaaga
    26521 ttgttgtgaa atgatactcg ttttgtaaag attttagcaa atcaataatc tgagcttgaa
    26581 tagttacatc caaggcagtt gttggctcat cacagatcaa gacatcaggt cggcaggcaa
    26641 gggcaatcgc aataacgata cgttgacgca ttcctccaga atattggaat gggtattcat
    26701 taaaacgtct atctgcgtct ggaatgccaa ccttattcat gtagtcaatg gccaattctt
    26761 tcgcttcttt agctgttttt ccttggtgtt ttacaataac ttctgtaatc tgactaccaa
    26821 ttgttttaat ggggtccaaa ctagtcattg ggtcctggaa gatagtcgca atcttagcac
    26881 cacgaatttg ttcccaatcc ttgtgagaag ataaagctgt caagtcctga ccacggtagt
    26941 caatactacc ttgggcaatg cgaccatttt cttcgagcat acctgtgaag gtctttgtca
    27001 aaacagattt acctgatcct gactcaccta ccaaggctaa tacttctcct tcgactagtt
    27061 caagggaaac gccgcgaatg gctgtcaata ctttgtcacg aacgtcaaat tccacgacaa
    27121 tatcgcgagc agtcaaaatt acattttttt cttttgtcat ttctactcct atctatgtgt
    27181 acgtggatca ctagcatccg ctaagttttg accaactacg aaaagggaca aggataccaa
    27241 gacaagggtt gtcaatggaa tccagaacaa gtaagcattg gttgttacgt tttgtgaata
    27301 atccgaaatc aaacgaccca aacttggcac tgtaatcggt aatccaagac cgaagaaaga
    27361 caagaaggct tcgtatgaga taaagcttgg aagcatttga gtcatggttg tcacaataac
    27421 agataccaat tgaggcatga tatttttggc aacaatcttc aaggttggtg ttcccaaagt
    27481 acgtgacgcc aagttgtatt ccaagtcacg atagcgcaag atttgcacac ggatcatgaa
    27541 ggcaatacca atccatgttg ttacgctcat ggcaaaaatc agattccaga atccagctcc
    27601 gattgagtaa gtcaagacaa taacaatcaa aagaggtggg atgtttgaga tgacgttgta
    27661 aacttccatc atgacacggt caactgattt tgaaataccc caaataccac cgacaaaaac
    27721 accgataacc aagttaatca ctgtcgcaat cacagaaatg aggatggagt tacgagctcc
    27781 gaaccagaca ccgtcaaaga gcgatttacc gttactgtca gtaccgaacc aatgctccgc
    27841 atttggcttg atataacgaa cactaaagtc gtttaccttg ctgacatcat tgaaatcaaa
    27901 cttagaaaac attgggtaga tgaaacttat caaaatgatg gctaccaaga ttcccaacat
    27961 gactacagtt gattttttct tcataaattg tttaaacact gatttccagt aagaatatgc
    28021 tggcgcatca atagtttcag aggcaaaatc gtcacgtttt acaaactgaa atttttcttt
    28081 atcgattgta gacattattt gcctcctttc tcagtcaatt taatacgtgg gtcaataata
    28141 gtcatccaaa tatctcccaa aagacgtgag aagatagaaa tacatgtaaa gatgaagaca
    28201 agaccaacga ccatagagtt attagatgct tttacagagt caatcaacat tttacccata
    28261 cctgggaagg cgaagactgt ttcagtaagg gttgcaccac cgataacccc aataatggca
    28321 ccaggaattc ctgaaaccag cggaaccatg gcatttttaa agatgtgttt gtttgaaatt
    28381 tctttttcag acaaaccttt tgcacgagcg aaacgaacaa agtcttgaga ttgcaagtca
    28441 atcatgtaac gacgaatcca aatggctgta ccaggagcac ccaacaaacc aaggatgact
    28501 gctggtaaaa cgtaagaacg ccaatctcca gctcccaaga tagggaatga atctggaagg
    28561 gcaatagatg atccaatcaa tcgaacgatg taaaccaagg caatcgttgg aagagcaagc
    28621 aagaaggtca aagcccctgt tgagaggcta tcaatccaag tgttcttgaa acgagccatg
    28681 gctgaaccaa gtggcacggc aagagcatag gcaagaacca aaccaatcaa accagtaata
    28741 gcagagctga caatcataga tggatattgg taattacttt cagtcgctgt ataaggatca
    28801 tctttcccat agctagctac ttcacgagag tcagcctgac taggtgactt gtaggttctt
    28861 gagtaaatat ttacagaaga cgttttctta cctgttggga actgaacttg ggcagttttg
    28921 gtttgtcctt gaccttgagt aataacctga agaactggtg tattagcata ggttgggtaa
    28981 gagtcaccta aattcaagtt cacaaagttt tgatgaacaa atgggaactg actgttaaag
    29041 tacaagagat atttatgttt agttcctgaa ccgaccaatg accatccgat agctggatca
    29101 ttttcaaaac gaaggtagcg tttcaagtct ggattttcag ggtcttggat tttatttgta
    29161 tggtcaatgt caatcaagtt agcatagaag tgaaaaacac gttcaaaaat tggaatttca
    29221 cgagtagcat agaattgacc actttcagta aattctccca aagtccaacc atgacctaat
    29281 tgattgatgt acttttcata aatagcttta ttggtcgcat ttgcttctac tgttacagaa
    29341 gaatccatgc tacttgcctt ttcttgcaac tctttagtat cgtaatactc aatgtagccc
    29401 atacgctcaa acacagtatt ttcatagtta tcacgtttat cagccgttgt cgcaatttta
    29461 ttatagttag gatcctgctt gaaaatcaat tttcgaggaa ccaaggtata gataatcgtg
    29521 taggtcaaag tcgttactaa gaaaatcgaa accaatgacc gcaaaacacg cataaaaata
    29581 tattttttca tattatttcc tttaaaaatc ccaaaagaac cttctcctca tggagagaaa
    29641 gttctattag aaattattta cttcacatga cttgccaatt ctttttgagc tttctcattt
    29701 gattcagctt tttctttcaa ccatttttca cgagcttttt catactcttc cttagtcacc
    29761 actttatctt gtaatttcaa atatttgaag taaacatctg accccttaga gcctgtttgc
    29821 gcagaagctc cagtaaatgg aacaattcgt gaaagcactg gtgctgcacc agaagaagcc
    29881 atagcaggaa taaagagtga actatctgtc aaccatgctt gagccgctgc atatttttca
    29941 taacggacat tcaagtcgct tgtctctctg gcagcttcat caactaattt atcgtattct
    30001 ttcaaaccaa cttgaactac tgaagggcta tttggattat caaatcctaa atatgttttt
    30061 gtagtttcac tgctagttgt ttttaaaata tccaggtaag tagatgggtc ttgatagtct
    30121 ggcccccatg aaactcctcc tgatacatcc caatcctcag atgaagcatt ggcagcatag
    30181 taagtaatat taaggaattc atcacttgtc atttgttgaa tatcaacaac gacattttca
    30241 acaccaagaa ctgtttctac agattgttta aaggactgaa tacgagatat gtagtttttt
    30301 gatgcttggt ctactggaac gtccagatga ataggaaact gaacgccgtc tgcttctaaa
    30361 gctttcttag ctttcgcaaa ctctgccttg gccttgtcag cattgaataa accatcctgc
    30421 ccatcagcta aattcacacc tttccactca tcaccataag caggaagttg agcagcgact
    30481 aaatcaccaa aggtcttctc accagctgaa acaaagtctg gttttacaaa taaattacga
    30541 actgctaaag ctgctccatc tttaccattg atttgagctg agtaagctga gcgatcaaga
    30601 gcaaaattca aggcttgacg gaaatctttg ttaagcaatg ccttcttagt agctactttc
    30661 tctgaatctg tagttttaga agtatagttg taactttggc gatcaatatt cacacccaga
    30721 ccagcaatcc cagagcctga ttgtgtgtaa tagatattgt ccttgtattc ttctgcaacc
    30781 ttagaatagt tggagctggt agggtaaaga cgggcataac tataagctcc actagtgaag
    30841 ttacgctcta gcgactcctg atctgatcca tcatagtaag ctagattgat agtatctagg
    30901 tggacatttt ctttatccca atattgctca ttttttacaa actctacaga agattttgca
    30961 gtcaaccctt tcaacaagaa tggaccatta taaagcaagg atgtcggatc tgttggttta
    31021 gcaaaatcgc ttccttttga tgtttcgaat tcttcattca gaggccagaa aatagaatag
    31081 gtcaacttag agttccagaa cggttcaggc tggttcaaag tgtattgtaa cgtataatca
    31141 tcaaccgcct tgacaccaac tgttgaaaaa tctgttgaag ttcctgatag ataatctgcc
    31201 aagcctttaa ccgaattttc agctaaatac atagcttctg attttttatc tgctgcgtgt
    31261 tttaaaccgt tcacgaaatc tttagccgtc acctctgcat attcttctcc atcagaggta
    31321 aaccatttaa cccctttacg aatcttataa gtgtaggtca aaccatcctt agagacttcc
    31381 caatcctctg caactgcagg agcaagatta ccgtaattat cgttagtgaa taaaccatca
    31441 atcccatttg aagtcactac tgttgtacta tttttacttg aaatcaggta gtccaaggtt
    31501 tctgggtctg ctgtataaac atagccataa gctttagggg ctgatgaatc agatgatttt
    31561 gaagaactgc atgctgcaag tacacctgct gctaataaaa caagacctgc tgtagcaaat
    31621 acacgatttt ttttcatttt ctactcctct gtttatgtga attatagatt gacaaccatt
    31681 atatcacatt atccattaaa aatcaaacaa attttcagaa tatttaggct tgttggcaca
    31741 aatttttcat tttttttgaa tatatgattc aaattgtcgt tcgaagtgtc aaagactaca
    31801 gtgaaaatag gaaatttgac gcagaaactt tggagtttag gaagacatac agtaaaatga
    31861 aatacggacg aaacaatgtg attttggaat tcaaattaaa ttataacaat attgtagaag
    31921 tatcattcta gtattcaaga ttcagtttac tatgtctttt cacaccaacc ttattccgaa
    31981 ttcaattact tttgtgattt acatatatag attaagacta tcttttatac tttaaaattt
    32041 ctcgctacct tatccactat atgctcctcg ctatcacgtt tctattcata gcctacgatt
    32101 tcactattgc tttctctgac aattcttatt tcctgcgtca gacttaaaac gatctatccc
    32161 cagaccattt taatccgcta cctcacgata gtcaggcttg gggagcgcta ttgtattcac
    32221 cggtagtgga tccctacaga ggacttacac ctcagatgca cgacatgccc atcgtataaa
    32281 aaaatctcct acccaaggta gaagatttca aacttataaa acttaatccg tcatgtccga
    32341 taccaacatt cgatgctcca atggaatact gcacataact agcaagaaaa taaagcctga
    32401 ctgaatccag aagagagcca agtcaaaaat tccgtgcaca gcaaccactg taaggaaaga
    32461 tagataaagg ccgataatcg gacgtttccc cgactcctga ctcatatcca tcatcaagcg
    32521 aacaggagca acagaagaca aaactaataa aatggtaccc acaattccgt aactcagaat
    32581 cgtatcaata taaagactgt gggcatgttc atgataagga gcatgtatcc gaggataaga
    32641 gtgcatatag gtcaatggcc cttcacccca aaaaggattt tgcttaaaca aggccatccc
    32701 agcatcccag atagaaatgc gttcttccat agaagagtct aaagtaccca ttcgaactcc
    32761 caaatcacta gaaaagagga aactcaaacc aatcgcgaag accccaatac taagccaaaa
    32821 ggccttccag tttttaatag tcgtaaagag atagataatt gctccagcga taatagcagg
    32881 aaaggcagtt cgattttgag taaagttcaa accaaagaga ttaacaaagc ctgcaatcac
    32941 acagaatact ttcaaccaat tcaacttggt cgttgtaaac agatagaaag caatcataat
    33001 acagaaacaa caaataattc cataataatt aggattaaag aaggtcactt ctgcccggtt
    33061 ctgatgccac acctgcatat tgggtgaaag aaaagcatag ttaaatttct tcacaatttg
    33121 gaaatgttct aaactggcaa aagcagctga caagacacta ccaaacaaga cgaactgcaa
    33181 aatcaatcga aagaatttat gggataaaat cgactgatag tgcaaaaaga aaatagtaaa
    33241 tagaaacatt cctactgaag ccacaagacc catccaattt tgtgcaagaa tggatataac
    33301 agtactatag ctaagaaaaa gaagcagcat cggatgctcc cccattttct gaagaatact
    33361 tttcatgtct cctgtaaaaa tcaaactgat aatatataaa cagagtacaa ctacaaaaag
    33421 ataaaagggt aaaaagatac ttaggataat tcccaataaa atcagctctt tactagacaa
    33481 ccccttcagc ttttcaataa agcctattga tttcaaaatg aatcctttct ctccaaatca
    33541 gctgattcag ataatagtaa gctgtcctat attgtaccac ttttttagca atttgaaaac
    33601 aaaggaaacg ttttccaaaa taaaaaccct attttatcca ccatatcaag gcttcaaaat
    33661 gttacttcaa ctccattctc aattacccga taagtctgat tttgcaaatc aatttctgct
    33721 actgctgtta cggacttatc tttattttga cgtttgatta caatgctgtg agctgttggt
    33781 gtctctatct cagtagtccc ttctagatca aaggcttctg aacggttacg gaaagaaaat
    33841 agattgagaa gggccttcac aacaggtcgt tgcacttctt ttgctatttc ctcgttgcta
    33901 tagtaatgac gattaatatt tcgaccttct ttagtttctt ctaataattt caagtcattc
    33961 ttgcctgcta atagacccac atagtaaacc tgaggaatac ctggggcaaa agcttgaatt
    34021 agacgagcga gaaaatactt gacatcatca tctccaagcg ctgaatagta ggttgaattg
    34081 atttggtaga tatctaagtt gttatactcg gcactagagt acttacgttt gacattggct
    34141 ccaaccttat agagttcatt tgaagcatag tcaatctcct catcggtcag gatatccttg
    34201 acatctacta ctccaatccc atcatgggta tctagcgtcg taaattgctt catcgggctc
    34261 atctttaacc acttagccaa acgctctgtt ctggaactgt aaagagtata aagtgtcacc
    34321 attggaagag caaaatcata aacatagtaa tcatggtctg ctattttaaa ctgaatcgaa
    34381 tagtgttcat gaatctcagg taaaagctct gtcccatact cagcagcgat atctcgaact
    34441 ttgtccaata aatcccaaat atctggttcc acaaagaaat cattagtatc caatttcttc
    34501 actgcataag caaaggcatc tagacgaatc aaatcacacc cattacttgc caagtgctga
    34561 atggtcttac ggataaattc catagttact tctttggtca catcaagatc aatctgctcc
    34621 tcaccaaagg tattccacaa atgttccact gaaccatctt caaacacaat ctcttgcttt
    34681 ggtgcacgat ccttacgctt gtaaattaaa tctacatcag actgtgtcgg acggttttct
    34741 ggccaaaact tatcccagtt taaaaagaga gctttaaatt cactggcttc atgtttttct
    34801 tgatagtcct tataatactt ggattgacga gaaatatgat taatcataaa atcaaacata
    34861 agataatatt tctcacctaa atgcttaaca tcctcccaat caccaaaagc tgagtccact
    34921 tcgtcgtagt caactggcgc aaatccacga tcacctgttg atgggaaaaa tggtaaaagg
    34981 tgaactcctc caatagcatc tccaaaatgc tcttccaaat tatcatataa gtctttaaga
    35041 ttatttccaa ggctatcaga ataggtaatc aacatggttt tattttgaat tggcatcatt
    35101 actctccttt ttctaattga agccaagtct catatgatct ggcttcataa ataaaattca
    35161 ttttaaatct ctatttatca tcaaactcgt actaatatag actgtgataa acaaagtact
    35221 actttcttgt tttctgcata gaattatcaa caagctaaac tcttcctctg tgtcaaagac
    35281 tatagattcc atgagctctt cttatactct tcgaaaatct cttcaaacca cgtcagcttc
    35341 accttgccgt aggtatggtt actgacttcg tcagtttcat ccacaacctc aaaacagtgt
    35401 tttgagcaac ctgcggctag cttcctagtt tgctctttga ttttcattga gtattacttc
    35461 actgccccgt tgctcattcc tgaaatgata tggcgttgga agaagagata gacaatggtg
    35521 atactgataa tgccgaccac gtaagaggca aagcttggtc cgtagtcgtt gaaatattgg
    35581 cctgcgtagt tgtattggaa caaaggcaga gtccacattt tggaatcccg gttcaagaca
    35641 aggagtggca acatgaagtc attccagaac caaagggcat tgatgatcat ggttgtcgca
    35701 tgcatcggtt tcatcattgg gaagatgatg cggaaatagg ttgtaaattg attagcccca
    35761 tcgatctctg ctgcttcatc cagactttct ggaatcgaga ttttgatata gccaacatag
    35821 agaaagaggg tctgtggaat cgcataggtc aagtagagca agatcaaacc aaaggtatta
    35881 gccaaaccga gtttactcat cataaccgta atcggaatca tgatgacttg gaaaggtacg
    35941 aagattccga ggattaagag ggtatacatg atggtaaagg cttttctttt actcatattg
    36001 cgagcgatgg agtaggctgc catagggata aagatcatta ctgcaagtaa agacaagaca
    36061 gtgatgacga cagagttcca ataatagcct ccaatcccat cagctaagag acggctaaag
    36121 ttgtcccatg tgaagttggt tggaaagcca aagaaattat ctacaatatc cttagtgggt
    36181 ttgaaggaac taaagagggt agcaaggagc ggcactaaaa tcagaaccga tcctagaatc
    36241 aatagaatgt atttgccaat cagggctttt ctttcatctt gtttcatcat gcttctcctc
    36301 ttaaatttca aatttcttag atactctcaa ttggatgatc gaaatcacta caattaagaa
    36361 gaacaagatt acggcaatgg cattggcata accgaattgg ttgtttttaa aggcatagtt
    36421 ataaaccaag agcccaagtg aggttgtggc attgtttgga ccaccaccgg tcatggcaaa
    36481 gacttggtca aaggcagtca gcccaccttt tagggctagg ataaagacca tagagacact
    36541 tggtagcaag taaggcaatt caatgttcca gaaaacttgc ttgctagtcg caccatcaat
    36601 ccttgctgcc tctgtaatct cagttggaat agattgcaaa ccagctagga agatgatgat
    36661 gggcatagcc accccttgcc aaagaaggac aaagacagcc gcaaagattg ctccccactt
    36721 agtccctaaa agactggttt ggaaaaattc aatatgaagg gcatttccaa tcgctggaag
    36781 accgtagttg aagacttgct tgaagatcaa agccactgtc aaaccagata aaacagctgg
    36841 gaagaagaac caagcacgga agaaggtttg gcctttgatt ttagaattca agacacgcgc
    36901 aatgaagatc ccgagtgcaa tctcaccaac caccatggca atcgcaatga ttgcggtaaa
    36961 gccaatcgca ttcatgaatt ttggatccat gaagaggagc ttaaagttgt ttaagccaac
    37021 aaatttgtag ttataagtca atcctgtcca gttggtaaaa ctgtaaaagg ctccttgaaa
    37081 catcggcaca tagaagaaaa ttgcttgtaa caagaggggg atgaccacaa aagcccatgc
    37141 ccaatatttt tgtaatactt ttttcatagt ctctctactc ctaatccaca tccgctttca
    37201 tcgggttaaa gaaggcattc aaatcattga ccatgccttg tttatcaccg gtcaagacat
    37261 agttcatggt caaggtatgg aagtctgctt cactggtcca gtattgttgc aaccagacca
    37321 agtgacgatc cgtaaaggca tattcggtca taccagcaag cggtgaatct tctcctgctt
    37381 gtttgacccc ttcgatcgct gttggagatc cgtccacatc gtagtatttt tgcatgactt
    37441 ctggacgggt catatattcc acaaaggcat tggcttcttt tggatgtttg gtggtggctg
    37501 agatagacca tgccaagtct cccgcaccaa cggttaagct ttgtcctttt tcttttcctg
    37561 gaatcatgaa ggtcccaatc ttaaagttcg gtttttgttc attaatcgct gtgatcgccc
    37621 aagacccatt tggtgtcatg aggacatccc cacgtgcgaa ggctccgata acatcggtat
    37681 agccagcacc ttcccagttc ttttgcttag agccattgat gcgaaggatg tccatgacct
    37741 tgatatcatc tttcataatc ggatccgaca atttaatggc atttggttga gaataacgaa
    37801 ggtattgatt tgcttctttt cctccacctg ttgctgtcgc aaaggctaat tgattgtaac
    37861 cattgagtgt ccaagcatct gcacctgcaa ttccaaatgg tgtttgtcct ttagcaacga
    37921 tatctttgac taactgttca aattcatccc aggtttcagg aaccttcaag cccagttctt
    37981 cgaatttatc tttgttgtag taaattccat aagcattagc tgtaaaagga acgttgtaaa
    38041 ctttttcgtt tacagcatat ttttcagcgt agccattttt cacgcgtttc aggtagtctt
    38101 tgttgctcaa atcttcaaaa acacctgctt ttgcccattc ttgcagttcg atggactgtg
    38161 ggtaaatatt gaccacatca ggcacatctc ctgcgagaac gcgtgtcttc aatacttcac
    38221 cagcatttgg tacattgacg actttgacct tgatcttagg gttttccttc tcaaaatcac
    38281 gagtgatttc ttccaaggtt ttggtcattt cttttttctg gttgaaatac tcgatggtca
    38341 ctgtgccatc cgcagattta ccatagttgg agcaagcgcc gagcccaaac aaagctaaac
    38401 ctgtagttgc aagaagtccg atttttttat accattccat tagaaagcct cctttataaa
    38461 tttatacacc cttattgaac tgcaccccaa aagttagaca gaataaatct aacttttggg
    38521 gtcagtacat atcatagttt tctaaaaata tactgtctac tcaaaaaatc tccttgggat
    38581 aagataacag ttaagcccgc atacattagt tctgcacctg agtaaacttc gccattttcc
    38641 tgtaatttat atagtccctc ttcatccaaa tcttttaatt ttaaagttgt ttccatggtc
    38701 tctacaacag ataaaacgcg aacgtaggtt acaatcgttt gatttccgta attaaattgt
    38761 acagctgctt cattggatac agtatcagga ttaattagtc tatactgctg tcctaactga
    38821 actactggtc gtaattcttt atacaagttc acctgattag caatcgtagc tttctcttca
    38881 tctgataaat ttgtcaaatc aagttcatag cccaaatttc ccatcattgc tacaaggcca
    38941 cgtgtttcta atggtgtcat tcgtcccatc tgatgattcg gtactgctga cacatgagcc
    39001 cccatagaaa tggttggata gagataggat gaaccgtatt gaattggtaa acgtgcaatg
    39061 gcatcagtat tatcactagc ccagacttgt gggaaatagc gcatcatacc aagatcattt
    39121 cgtccaccac caccagagca ggactcaaag agaatatggc tgtgcttctc tgtcagataa
    39181 gaaacgagtt cataaagccc cagcatgtac tgatgagatt gcatctgtgt ctctagataa
    39241 gttaatccat tccctagctt agtgatattg cggttcatat cccatttaat gtaatcaata
    39301 tcatgataaa ataggagttg atctaagaca cttttcaagt attctactac ctgaggattg
    39361 gcaagattaa gtactaattg attccgagaa taagtatgct catagccagg aacctgaata
    39421 gcccagtcag gatgttgacg atacaaatca ctatctacag aaatcatttc gggttctaac
    39481 caaagtccaa actgcaaacc tctttcatgg atagctgaaa tcagactttc tagacttcca
    39541 cccagttttt cctcattaac aacccaatca cctaaagcac gattatcatc aaaacgattg
    39601 ccaaaccaac catcatctaa tacaaaaagt tcaatgccaa ctttcttagc ttcatctgct
    39661 aactctaaca gtttttctct ctgaaagtca aagtaagtag cttcccagtt attgattaga
    39721 attggacgtt cttttttaga aaattcactt agcataatgt gcttcagtac aaaattctga
    39781 ctttcatgac taataccagt taatccctga tctgaatgag tcactaaagc taccggtgtt
    39841 tcaaagtatt cctcaggagc taacttccaa gaaaagtttt ctggattaat gccaatagcc
    39901 acccgaactt cattcaattg atttttttga acaaaagctt caaagttgcc actatacatt
    39961 agttgaatag caaacacatt cccagcatcc tctgtgactc cttgttcgca tagtagaaga
    40021 gctggtgttt gagcatgacc agaagcacct cggtttgaac taatcgaaaa gattccttgt
    40081 tctacctgtt gacgtctaac agtcttttca cgagcataag caccctgcag agttactatt
    40141 tcgtaatctg cagctggaaa atcagccata aaagaaaaat ctttatggat gacaacttcc
    40201 tgattactat tattatctaa tttactgtag ctagcaatag tcgcatcatt attaaaagta
    40261 gtataataca aagtcagact aagttgagcc ttagaatctt ctaacattaa gacaagagtc
    40321 tctgtatcgt ccatgctatg tggagaaggt aagccctgtg gaccattctg accttttaaa
    40381 atctttgctt ctacaaatcg aaagtctgtt acttcagtta cactatgctg aacctgtatg
    40441 gttggtttcc taaaatctcc taagccatgt tgtccaaaaa tctgtcgctg agtatctaaa
    40501 ctaaaggttc gattagtagc cgttggattt cctgaaaagg catggtctcg ttcataaaca
    40561 ctattggaac ctttatagtt cttaatagtc tttcctaaat gtttcaaaag taagtagcca
    40621 tttcgatttt caataatcaa acttagattt ttactctcaa cataaaatag attattctct
    40681 atcctaactc ccatttactt cacctcatca ctttattgat tatattttat cacctgaaat
    40741 cgctttccaa aatagaaaaa tgtctcaaga atatggtaaa atgttaggta ggaggtagca
    40801 catgttagtt ttttcagaat accagactgg aacaatcgac cttgccctaa gcttttatgg
    40861 atatgaggaa tgcacaccta attactcttt tggtccagcc attcgtgata catacgttct
    40921 acattacatt actaaaggac aaggaaaatt tcattacaag ggtaaaattg ttgatttaaa
    40981 agaaggagat ttctttctat taaaaccaga ggaactaacc ttttatcaag cagatagtaa
    41041 agaaccttgg gcctactact ggttaggaat cactggaggg aaagcccctg attattttgc
    41101 tctttcccaa atttctgatc aatcctatct catccaatct gaaacttgtc atacccagac
    41161 tactgcaaaa ctcatctcag acattgtccg cttcgctcag attacaaaat caagtgaatt
    41221 agctcaactc catatcatgg gacaacttca tgaactgatg tttcatctgg gaactattgc
    41281 tcccaatcag aaaaaaaaga atatttcatc aacccaccaa ctctatcttg aatgcaaacg
    41341 attaattgat agccactatc ctcaatcact tacaattcaa gatttagcaa aagaactatc
    41401 cgttcacaga agctacttat caagcgtatt caaagaattt aataccttat cacccaaaga
    41461 atacctactc tacgttcgaa tgcaccgagc tagacaactt ctcgaaaata cccaagagtc
    41521 catcaaggta attgcatact cggtaggttt ttcagatcca ctccattttt cgaaagctta
    41581 taaacaatac tttaatcaga ctccaagtca tacaagaaaa gaatactctc aataccaact
    41641 agtaagaaag gcaacattat gaaatcctac caagctgtct accaaatcct atctaaagaa
    41701 accgactata tcagcggaga aaaaatcgca gaaaaactat ccctaagccg aacagcaatt
    41761 tggaaagcca tcaagcgact agaacaagaa ggcattgaaa ttgatagtat caaaaataga
    41821 ggatataaac tgatgaatgg tgaccttatt cttccagaga ttctagaaga aaatcttcca
    41881 attaaagtca gctttaaacc cgaaacaaaa tcaacacaac tagatgcaaa agaagcaatt
    41941 gatttaggcc atgaagcaaa taccctctat ctagcttcct atcaaacagc aggccgaggc
    42001 cgttttcaac gttccttcta ctcaccacaa ggtggtattt atatgacact ccatcttaaa
    42061 ccaaatctcc cctatgacaa attaccatcc tacacactac ttgtagctgg agctgtctac
    42121 aaagccatta agaacctaac tttaatagat gtcgacataa aatgggtcaa tgatatctat
    42181 ctaaacaatc ataaaattgg aggaatcctt actgaagcaa tgacctctgt agaaactggc
    42241 ttagtcacag atatcattat tggagtaggt atcaatttca ctattaaaga cttccctcag
    42301 gaattaaaag aaaaagctgc cagcttattt aaagctacag ctcctataac aaggaatgaa
    42361 ttgatcatag aaatctggcg tgctttcttc gaaacaccag cagaagagct attataccta
    42421 tacaaaaaac agtcattcat tctaggaaaa gaagtcactt tcacactaga gcaaaaagac
    42481 tacaagggac ttgctaaaga catctcagaa aatggaaaac ttttagttca atgtgataac
    42541 ggaaaagaaa tctggctaaa tagtggcgaa atttctctca atagttggaa gtaaaataac
    42601 acaattataa tataaacgat ataaaaataa cttcagatta gtaattcaat taagttttac
    42661 ggatctgaag ttttattggc tctaaaaata aaaaagagag ttacagactc tcattaaaac
    42721 ggagaataag ggattcgaac ccttgcgcca gttacccgac ctaacgattt agcaaaccgt
    42781 cctcttcagc ctcttgagta attctccaat taatgggcac gagtggactc gaaccaccga
    42841 cctcacgctt atcaggcgtg cgctctaacc aactgagcta cgcgcccaag ttaaaaaact
    42901 tggtaatttg aacaaagttc aaagcgggtg acgagaatcg aactcgcgac aacagcttgg
    42961 aaggctgtag ttttaccact aaactacacc cgcataaata ctatcaataa aatggcgcga
    43021 gacggaatcg aaccgccgac acatggagct tcaatccatt gctctaccaa ctgagctacc
    43081 gagccttatt gcgggagcag gatttgaacc tacgaccttc gggttatgag cccgacgagc
    43141 taccgagctg ctccatcccg cgttaatata aaaggaggat gtgggattcg aacccacgca
    43201 cgcttttaca cgcctgacgg ttttcaagac cgttcccttc agccggactt gggtaatcct
    43261 ccaatattca aatggacctt gtaggacttg aacctacgac cactcggtta tgagccgaga
    43321 gctctaacca gctgagctaa aggtccgaca agatcattat agcggcgaag gggatcgaac
    43381 ccccgacctc ccgggtatga accggacgct ctagccagct gagctacacc gc
//
LOCUS       DAVBNO010000015        40539 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVBNO010000015 DAVBNO010000000
VERSION     DAVBNO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40539
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.015"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            3..75
                     /locus_tag="VQ846_000853"
     tRNA            3..75
                     /locus_tag="VQ846_000853"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Val,seq:tac)
     gene            81..151
                     /locus_tag="VQ846_000854"
     tRNA            81..151
                     /locus_tag="VQ846_000854"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:113..115,aa:Gly,seq:tcc)
     gene            186..259
                     /locus_tag="VQ846_000855"
     tRNA            186..259
                     /locus_tag="VQ846_000855"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:Ile,seq:gat)
     gene            342..431
                     /locus_tag="VQ846_000856"
     tRNA            342..431
                     /locus_tag="VQ846_000856"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:378..380,aa:Ser,seq:tga)
     gene            445..518
                     /locus_tag="VQ846_000857"
     tRNA            445..518
                     /locus_tag="VQ846_000857"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:479..481,aa:Met,seq:cat)
     gene            522..594
                     /locus_tag="VQ846_000858"
     tRNA            522..594
                     /locus_tag="VQ846_000858"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:555..557,aa:Phe,seq:gaa)
     gene            607..687
                     /locus_tag="VQ846_000859"
     tRNA            607..687
                     /locus_tag="VQ846_000859"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:641..643,aa:Tyr,seq:gta)
     gene            692..762
                     /locus_tag="VQ846_000860"
     tRNA            692..762
                     /locus_tag="VQ846_000860"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:724..726,aa:Trp,seq:cca)
     gene            777..849
                     /locus_tag="VQ846_000861"
     tRNA            777..849
                     /locus_tag="VQ846_000861"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:810..812,aa:His,seq:gtg)
     gene            863..934
                     /locus_tag="VQ846_000862"
     tRNA            863..934
                     /locus_tag="VQ846_000862"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:895..897,aa:Gln,seq:ttg)
     gene            944..1027
                     /locus_tag="VQ846_000863"
     tRNA            944..1027
                     /locus_tag="VQ846_000863"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:978..980,aa:Leu,seq:caa)
     gene            1134..2411
                     /locus_tag="VQ846_000864"
     CDS             1134..2411
                     /locus_tag="VQ846_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET3075459.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2487..3971
                     /locus_tag="VQ846_000865"
     CDS             2487..3971
                     /locus_tag="VQ846_000865"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET3075460.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            4032..5312
                     /locus_tag="VQ846_000866"
     CDS             4032..5312
                     /locus_tag="VQ846_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET3075461.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKLTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVAMA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5316..5936
                     /locus_tag="VQ846_000867"
     CDS             5316..5936
                     /locus_tag="VQ846_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET3075462.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6102..7280
                     /locus_tag="VQ846_000868"
     CDS             6102..7280
                     /locus_tag="VQ846_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET3075463.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASNSEVAETV
                     TAAEEVALSTDSTTPEGQPAETTSPVEEVAPQATTLAEKQETQVSSQTESAVEATTMP
                     VEEKATEATTTSSEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVTK
                     SENAGLQPQTAAFKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIA
                     EYAIQNMASRGISYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG
                     "
     gene            7793..8131
                     /locus_tag="VQ846_000869"
     CDS             7793..8131
                     /locus_tag="VQ846_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET3075464.1"
                     /translation="MTRYQILLFLLEHSPCNQMAVQERLKIDQAALTRNFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            8142..8510
                     /locus_tag="VQ846_000870"
     CDS             8142..8510
                     /locus_tag="VQ846_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET3075465.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            8617..9261
                     /gene="pcp"
                     /locus_tag="VQ846_000871"
     CDS             8617..9261
                     /gene="pcp"
                     /locus_tag="VQ846_000871"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET3075466.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(9289..10146)
                     /locus_tag="VQ846_000872"
     CDS             complement(9289..10146)
                     /locus_tag="VQ846_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET3075467.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10276..11418
                     /gene="tgt"
                     /locus_tag="VQ846_000873"
     CDS             10276..11418
                     /gene="tgt"
                     /locus_tag="VQ846_000873"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET3075468.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            11519..13336
                     /locus_tag="VQ846_000874"
     CDS             11519..13336
                     /locus_tag="VQ846_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET3075469.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13489..14640
                     /gene="nagA"
                     /locus_tag="VQ846_000875"
     CDS             13489..14640
                     /gene="nagA"
                     /locus_tag="VQ846_000875"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET3075470.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14803..15861
                     /locus_tag="VQ846_000876"
     CDS             14803..15861
                     /locus_tag="VQ846_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET3075471.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            16012..16377
                     /locus_tag="VQ846_000877"
     CDS             16012..16377
                     /locus_tag="VQ846_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET3075472.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16453..17394
                     /locus_tag="VQ846_000878"
     CDS             16453..17394
                     /locus_tag="VQ846_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET3075473.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17342..18358
                     /locus_tag="VQ846_000879"
     CDS             17342..18358
                     /locus_tag="VQ846_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET3075474.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVIQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMAGLVSVLALLALTFYKRSSKMNVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18360..18686
                     /gene="comGC"
                     /locus_tag="VQ846_000880"
     CDS             18360..18686
                     /gene="comGC"
                     /locus_tag="VQ846_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HET3075475.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKDDGRITEEQVKAYKEYNDKNVGA
                     NRKVND"
     gene            18679..19083
                     /locus_tag="VQ846_000881"
     CDS             18679..19083
                     /locus_tag="VQ846_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET3075476.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTISNGSQKLPVPKGIQVPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            19088..19348
                     /locus_tag="VQ846_000882"
     CDS             19088..19348
                     /locus_tag="VQ846_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075477.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19311..19772
                     /locus_tag="VQ846_000883"
     CDS             19311..19772
                     /locus_tag="VQ846_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET3075478.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19750..20163
                     /locus_tag="VQ846_000884"
     CDS             19750..20163
                     /locus_tag="VQ846_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET3075479.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            20300..20894
                     /locus_tag="VQ846_000885"
                     /pseudo
     CDS             20300..20894
                     /locus_tag="VQ846_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20955..21908
                     /locus_tag="VQ846_000886"
     CDS             20955..21908
                     /locus_tag="VQ846_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET3075480.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKHLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSNLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21959..23149
                     /locus_tag="VQ846_000887"
     CDS             21959..23149
                     /locus_tag="VQ846_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET3075481.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAESFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23298..23669
                     /gene="rnpA"
                     /locus_tag="VQ846_000888"
     CDS             23298..23669
                     /gene="rnpA"
                     /locus_tag="VQ846_000888"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET3075482.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23644..24468
                     /locus_tag="VQ846_000889"
     CDS             23644..24468
                     /locus_tag="VQ846_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET3075483.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24487..25473
                     /locus_tag="VQ846_000890"
     CDS             24487..25473
                     /locus_tag="VQ846_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET3075484.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSRTFYVTINVNDYVEHRAEVLQTYAQKL
                     ATRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25524..26147
                     /locus_tag="VQ846_000891"
     CDS             25524..26147
                     /locus_tag="VQ846_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET3075485.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26278..27735
                     /locus_tag="VQ846_000892"
     CDS             26278..27735
                     /locus_tag="VQ846_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET3075486.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27758..28039
                     /locus_tag="VQ846_000893"
     CDS             27758..28039
                     /locus_tag="VQ846_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET3075487.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            28116..28601
                     /locus_tag="VQ846_000894"
     CDS             28116..28601
                     /locus_tag="VQ846_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET3075488.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28618..29283
                     /locus_tag="VQ846_000895"
     CDS             28618..29283
                     /locus_tag="VQ846_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET3075489.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29287..30150
                     /locus_tag="VQ846_000896"
     CDS             29287..30150
                     /locus_tag="VQ846_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET3075490.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAVQEAQAF
                     LYPLIKKAGLM"
     gene            30152..30856
                     /locus_tag="VQ846_000897"
     CDS             30152..30856
                     /locus_tag="VQ846_000897"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET3075491.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            31035..32708
                     /locus_tag="VQ846_000898"
     CDS             31035..32708
                     /locus_tag="VQ846_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET3075492.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32820..33911
                     /gene="ulaG"
                     /locus_tag="VQ846_000899"
     CDS             32820..33911
                     /gene="ulaG"
                     /locus_tag="VQ846_000899"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET3075493.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            34025..36001
                     /gene="tkt"
                     /locus_tag="VQ846_000900"
     CDS             34025..36001
                     /gene="tkt"
                     /locus_tag="VQ846_000900"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET3075494.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            36118..36417
                     /gene="yajC"
                     /locus_tag="VQ846_000901"
     CDS             36118..36417
                     /gene="yajC"
                     /locus_tag="VQ846_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET3075495.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36463..36891
                     /locus_tag="VQ846_000902"
     CDS             36463..36891
                     /locus_tag="VQ846_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET3075496.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36893..37303
                     /locus_tag="VQ846_000903"
     CDS             36893..37303
                     /locus_tag="VQ846_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075497.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37602..40253
                     /gene="adhE"
                     /locus_tag="VQ846_000904"
                     /gene_synonym="adhC"
     CDS             37602..40253
                     /gene="adhE"
                     /locus_tag="VQ846_000904"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET3075498.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12293 a         7268 c         9129 g        11849 t
ORIGIN      
        1 tagggagttt agctcagctg ggagagcatc tgccttacaa gcagagggtc agcggttcga
       61 tcccgttaac tcccatttta gcgggtgtag tttagtggta aaactacagc cttccaagct
      121 gttgtcgcga gttcgattct cgtcacccgc tttgaacttt gttctttgta ccaagttttt
      181 gacttgggcg cgtagctcag gtggttagag cgcacgcctg ataagcgtga ggtcggtggt
      241 tcgagtccac tcgtgcccat agtgtttagt ccattactag gggattggaa tattatctgt
      301 tcactaagag gacacgggct tgttcccgta taaactattt tggaggatta cccaagtccg
      361 gctgaaggga acggtcttga aaaccgtcag gcgtgtaaaa gcgtgcgtgg gttcgaatcc
      421 cacatcctcc ttttatatta ttaacgcggg atggagcagc tcggtagctc gtcgggctca
      481 taacccgaag gtcgtaggtt caaatcctgc tcccgcaata aggctcggta gctcagttgg
      541 tagagcaatg gattgaagct ccatgtgtcg gcggttcgat tccgtctcgc gccatttata
      601 tattttggaa gggtagcgaa gaggctaaac gcggcggact gtaaatccgc tccttcgggt
      661 tcgggggttc gaatccctcc ccttccattt tacgggcata gtttaaaggt agaactaagg
      721 tctccaaaac cttcagtgtg ggttcaattc ctactgcccg tgttaataga attatggcgg
      781 gtgtggtgaa gtggttaaca caccagattg tggctctggc atgcgtgggt tcgatcccca
      841 tcactcgcct attttatatt attggggtat agccaagcgg taaggcaagg gactttgact
      901 ccctcatgcg ttggttcgaa tccagctacc ccagttacta tttgccggcg tggcggaatt
      961 ggcagacgcg ctggactcaa aatccagtgt ccgcaaggac gtgccggttc gaccccggcc
     1021 gccggtatag tatagtgtta ggaacgttgt tattcttcgt tcctttttta tattattttt
     1081 ggtataatta tagttattca aattttattt agattaagaa agtgtagggt agtatgtctt
     1141 gttctatcga tttattaaaa catcggtatt tgaaaaatat taaagaaaat cctgaattgt
     1201 ttgtcggaat tgagttggag tatcctgttg caagtttaga aggggatgct acagatgttg
     1261 aagttataaa ggatctattt cattatttag tttctacttt ggatctcacc gtagcaaagg
     1321 tagatgattt tggcaatctg atccagttag tagatccgat aagtcaggat gctattttat
     1381 ttgaagtttc ctatacaacg attgagtttg catttggtaa ggctgaaacg attcaagagg
     1441 tcgaaaatcg tttcaataat tatatgaatg taattcagag aaagttagct gaatcaaatc
     1501 atgctattgt tggctgtggt atccatccca actgggataa aaatgagaat tgtccagtgg
     1561 cttatccacg ctatcagatg ttgatggatt atttgaattt gagtagaaat attattaaat
     1621 cagatttaca tcatttccct gaatatggta cttttatctg tgggagccag gttcagctgg
     1681 atatttcaaa aaccaactac ttacgggtga ttaatgcttt tactcaaatt gaagcggcta
     1741 aggcttattt atttgcaaac tctgaatttt cgggtgcgga ttgggatacg aaaatttcaa
     1801 gggatatttt ctgggaagaa tctatgcatg gtatctatcc agagaatgtt ggggtcaatg
     1861 ctagactcct taatgatgaa gctgattttt ttgactatct aaatcattct gcgattttta
     1921 ctgcggaacg tgatgggcag acctattatt tttatcctat tcaggctggg gactatttgg
     1981 ctacgtccga aatccaagca tttgctctga atggggatga ggttattatt tacccccaag
     2041 agaaggattt tgaaactcat cgtagttacc agtaccaaga tttaacgact cgaggaacag
     2101 ttgagtttcg tagtgtgtgt acacagccac ttgataggac ttttgcttct gcagcttttc
     2161 acttgggatt attggttaat ttagacaagt tagaagctta cttagaaaca gcacctttct
     2221 ttaaagtatt tggttatgat tacaagtctt taaggagaca attttctaag aaaaatctta
     2281 cagatgagga agaaactacg attattgaat tttccaaaga cttactccta ctagctgagg
     2341 agggactagt ggtgagaaat aaggaagaaa tgacctattt acagcctttg agagaagaat
     2401 tgagcctata atttctctta taaagggaga attttctgaa aaatcatgat ataatggacg
     2461 agactataga taaaggatag agagtaatga cattagttta tcaatcaacg cgtgatgcca
     2521 acaatacagt aactgccagc caagcaattt tgcaaggttt ggcgacggac ggcggtttgt
     2581 ttacaccggt tacttatcca aaggtagatt tggactttga caaattgaaa gatgcttctt
     2641 accaggaagt tgctaagcta gttttgtcag catttttaga tgactttaca gctgaggagt
     2701 tggactactg tatcaacaat gcctacgata gcaaatttga tactccagct attgcaccat
     2761 tagtgaaatt agatgggcaa tacaatttgg aacttttcca tggttcaacg attgccttta
     2821 aggatatggc cttgtctatt ttgccatact ttatgacgac tgctgctaag aaacatggtt
     2881 tggagaacaa gattgttatc ttgacagcga catctggtga cacggggaaa gctgctatgg
     2941 cggggtttgc ggatgtgcct ggtactgaga ttatcgtctt ttatccaaag ggtggtgtca
     3001 gcaaggtaca agagttgcaa atgaccactc agaatggtga caatactcat gttattgcta
     3061 ttgatggtaa ttttgacgat gcgcaaacaa atgtgaagca catgtttaac gacgtggctc
     3121 ttcgtgaaaa attgactacc aacaagttgc aattttcatc agctaactct atgaacattg
     3181 gtcgtctggt gccacaaatt gtttattatg tttatgctta cgctcaattg gttaagactg
     3241 gtgaaattgt agctggtgaa aaggttaact tcacagtacc aacaggaaac tttggaaata
     3301 tcttggctgc cttttatgcc aaacaaatcg gcctaccagt tggcaagcta atctgtgctt
     3361 caaatgacaa taatgtcttg acagacttct ttaaaacacg tgtctatgac aaaaaacgtg
     3421 agtttaaggt aacaaccagc ccatctatgg atatcttggt atcttcaaac ttggagcgct
     3481 tgattttcca tcttttggga aataatgctg aaaagacagc tgaacttatg aatgccttga
     3541 acacgcaagg acaatataag ttgacagact ttgatgcaga gattttggac ctctttgcag
     3601 ctgaatatgc gactgaggaa gaaacagcgg cagaaatcaa gcgtgtttat gagtcagatt
     3661 cttatattga ggatccacat acggcggttg cctcagcagt ttatagaaaa taccaagcgg
     3721 ttactggaga tgtaactaag acagtgattg cttcaacagc tagtccatac aagttcccag
     3781 tagttgcagt agaagctgta actggaaaag caggtttgac agactttgaa gccttggctc
     3841 aattacatga aatctcaggc gttgcagtgc caccagcagt tgatgggctt gaaatagctc
     3901 caattcgtca caagacaaca gtggcagctg ctgacatgca agcagcggtt gaggcttatt
     3961 taggacttta agacagaggg agcaaactcg gttgggaaac caactgagtt tcttttcatc
     4021 aggaggagag attgtttaag aaaaataaag acattcttaa tattgcattg ccagctatgg
     4081 gtgaaaactt tttgcagatg ctaatgggaa tggtggacag ttatttggtt gctcatttag
     4141 gattgatagc tatttcaggg gtttcagtag ctggtaatat tatcaccatt tatcaggcga
     4201 ttttcatcgc tctgggagct gctatttcca gtgttatttc aaaaagcata gggcagaaag
     4261 accagtcgaa gttggcctat catgtgactg aggcgttgaa gattacctta ctattaagtt
     4321 tccttttagg atttttgtcc atcttcgctg ggaaagagat gataggactt ttggggacgg
     4381 agagggatgt agctgagagt ggtggactgt atctatcttt ggtaggcgga tcgattgttc
     4441 tcttaggttt aatgactagt ttgggagcct tgattcgtgc aacgcataat ccacgtctgc
     4501 ctctctatgt tagtttttta tccaatgcct tgaatattct tttttcaagt ctagctattt
     4561 ttgttctgga tatggggata gctggtgttg cttgggggac aattctgtct cgtttggttg
     4621 gtcttgtgat tttgtggtca caattaaaac tgccttatgg gaagctaact tttggtttag
     4681 ataaggaact gttgaccttg gctttaccag cagctggaga gcgacttatg atgagagctg
     4741 gagatgtagt gatcattgcc ttggtcgttt cttttgggac ggaggcagtt gctgggaatg
     4801 caatcggaga agtcttgacc cagtttaact atatgcctgc ctttggtgtc gctatggcaa
     4861 cggtcatgct gttggcccga gcagttggag aggatgattg gaaaagagtt gctagtttga
     4921 gtaaacaaac cttttggctt tctctgttcc tcatgttgcc cctgtccttt agtatatatg
     4981 tcttgggtgt accattaact catctctata cgactgattc tctagcggtg gaggctagtg
     5041 ttctagtgac actgttttca ctacttggta cccctatgac gacaggaaca gtcatctata
     5101 cggcagtctg gcagggatta ggaaatgcac gcctcccttt ttatgcgaca agtataggca
     5161 tgtggtgtat ccgcattggg acaggatatc tgatggggat tgtgcttggt tggggcttgc
     5221 ctggtatttg ggcagggtct ctcttggata atggttttcg ctggttattt ctacgctatc
     5281 gttaccagcg ctatatgagc ttgaaaggat aggaaatgca aaaaacagct tttatttggg
     5341 atttagacgg gactttattg gactcttacg aagcgatttt atcagggatt gaggagactt
     5401 ttgctcagtt ttctattcct tatgataagg agaaggtgag agagtttatc ttcaagtatt
     5461 cggtgcaaaa tttgcttgtg cgggtggcag aagatagaaa tctggatgtt gaggtgctaa
     5521 atcaggtgcg tgcccagagt ctggctgaga agaatgctca ggtagttttg atgccaggtg
     5581 cgcgtgaggt gctagcttgg gcagacgaat caggaattca gcagtttata tatactcata
     5641 aggggaacaa cgcttttacc attctcaagg acttgggggt ggaatcctat tttacagaga
     5701 ttttaaccag tcagagtggc tttgtgcgga agccaagtcc agaagcggct acctatctgc
     5761 tagataagta tcagttgaat tctgataata cttattatat aggggatcgg actctggatg
     5821 tggaatttgc ccagaatagt gggattcaaa gtatcaactt tttagagtct acttatgaag
     5881 ggaatcacag gattcaagcg ttagcagata tttcccgtat ttttgagact aagtgataaa
     5941 aagattgtgt cagttttgtg acagagacct aacaaactat ttcaagtaat ctagtttgtt
     6001 acaaggaata gacagttctg ttaaataggc ccgagagggc tttttttcta cattttttgt
     6061 gttatgatag acaggtactc atttgaaagg aatttgaagg aatgaagaaa agaatgttat
     6121 tagcgtcaac agtagccttg tcatttgccc cagtattggc aactcaagca gaagaagttc
     6181 tttggactgc acgtagtgtt gagcaaatcc aaaacgattt gactaaaacg gacaacaaaa
     6241 caagttatac cgtacagtat ggtgatactt tgagcaccat tgcagaagcc ttgggtgtag
     6301 atgtcacagt gcttgcgaat ctgaacaaaa tcactaatat ggacttgatt ttcccagaaa
     6361 ctgttttgac aacgactgtc aatgaagcag aagaagtaac ggaagttgaa atccaaacac
     6421 ctcaagcaga ttctagtgaa gaagtgacaa ctgcgacagc agatttgaca accaatcaag
     6481 tgaccgttga tgatcaaact gttcaagttg cagatctttc tcaaccaatt gcagaagctc
     6541 caaaagaagt agcatcaaat tcagaagttg cagagacagt gactgctgca gaagaagtgg
     6601 cactatctac agactctact acgccagagg gacaaccagc cgaaacaact agtccagttg
     6661 aagaagtagc tcctcaggca acgactctag ctgagaagca ggaaacacaa gtgagctctc
     6721 aaaccgagtc agcagtggaa gcaactacaa tgccagtaga agaaaaagca accgaagcaa
     6781 ctacaacaag ttcagaagca aaagaagtag catcatcaaa tggagctaca gcagcagttt
     6841 ctacttatca accagaagag acgaaaataa tttcaacaac ttacgaggct ccagctgcgc
     6901 ccgattatgc tggacttgca gtaacaaaat ctgaaaatgc aggtcttcaa ccacaaacag
     6961 ctgcctttaa agaagaaatt gctaacttgt ttggcattac atcctttagt ggttatcgtc
     7021 caggagacag tggagatcac ggaaaaggtt tggctatcga ttttatggta ccagaacgtt
     7081 cagaattagg ggataagatt gcggaatatg ctattcaaaa tatggccagc cgtggcatta
     7141 gttacatcat ctggaaacaa cgtttctatg ctccattcga tagcaaatat gggccagcta
     7201 acacttggaa tccaatgcca gaccgtggta gtgtgacaga aaatcactat gaccacgttc
     7261 acgtttcaat gaatggataa acccgacttg gtaacatcat tttgacgaat gagatctagc
     7321 tttcgtgata ggaagcgatt ctcgttcgtt ttttctttgt catactcttc gaaaatctct
     7381 tcaaacaacg tcagttttat ctgaaacctc aaagctgtgc tttgagcaac ctgcgactag
     7441 cttcctagtt tgctttttga ttttcattga gtatcaattt gaatggaaaa tggaaagtta
     7501 tcatcttgta atgagttaag caacattctt gcaatctatt ttatagtaga ttgaaactag
     7561 aatagtacac aactgcttct aaaacattgt tagaaatcga tttgactgtc ctgatcgatt
     7621 tgtcctgctc ttatttcatt tcactatact ttatattata attgactggt caaatattga
     7681 taaatcaata aaaagagagg ggaagaaatg ctagagattc aagatttact gtatcaactc
     7741 cgcttgtctg agcaagtgag tacgcaattg tttgaaaaag cttgggatta gtttgacacg
     7801 gtatcagatt ttactgtttt tgctggagca ttctccttgt aaccaaatgg cggttcagga
     7861 gcgtttgaaa attgatcagg ctgctttgac acggaatttc aagattttgg aaacggaagg
     7921 tttggtggag cgtcatcgta atcctgaaaa tcagcgggaa gtattggtag aggctgcgaa
     7981 gtatgccaag gagcagttag tggtgaatcc ccctctgcag catatcaagg ttaaggaaga
     8041 gatggaaagt atcttaacag agtttgagag aacagaactc agccgtttat taaataaatt
     8101 ggttttgggt attgaaaata tagaaattta aggagaaata gatgtcaatt attacaatta
     8161 ttttaacaac gatcgttgct ttggagcatt tttacatttt ttatttggaa agtattgcca
     8221 cgcaatcaga tgcgactagt cgtgtattta atatggaaaa ggaagaattg gctcatccgt
     8281 cagtaagttc attgttcaaa aatcaaggga tttataaggc tctgctagga gtctttctct
     8341 tgtatgtcat ttatttctca cagaatttag aaattgtgac tatttttgtc ttatttgtga
     8401 ttggtgctgc gacttacggc tctttaacag cggataaaaa aattattttg aagcaaggtg
     8461 gatcagctat tttggccttg attagtattt tactctttaa atacacttga aggtcgattt
     8521 taatctcgct aatccttttt aatccagaat aagggaaata tgttatactt gtttttaaga
     8581 aaaaagtctc attgaattgg ttttgaggag ttagaaatga aagtattagt gacaggtttt
     8641 gagccctttg gaggggaaaa gggcaatcca actttggagg ccattaaagg tttaccagct
     8701 gaaatccatg gtgctgaggt ccgttggcta gaggtgccga cagtttttca caaatctgct
     8761 caagtattgg aagaagagat gaatcgttat caacctgact ttgtcctttg tattgggcaa
     8821 gctggtggaa gaactagtgt gacacctgaa cgagtggcca ttaatcaaga cgatgcacgc
     8881 atttctgata acgaagataa tcaaccgatt gaccgtccca ttcgcccaga tggtgcttcg
     8941 gcctacttta gtagtttgcc gattaaagcg atggttcaag ctataaaaaa agagggctta
     9001 ccggcctctg tttccaatac ggcagggact tttgtctgca gccatttgat gtatcaggct
     9061 ctctatttgg tagaaaagaa atttccatat gttaaggcag gttttatgca tattccttat
     9121 atgatggaac aggtggtgaa cagaccgact actccaacta tgagtttagt ggatattcgg
     9181 cgagggatag aagcagcaat cggcgctatg atagaacatg gagatcagga actcaagttg
     9241 gtaggcggag aaactcattg atagaaaaaa gcttgagggg aaaaaccttc aagcttttgg
     9301 acgttttcga gccaatactg ctcggtaaaa cataatttta gtgcattgga tataaggtag
     9361 gagtgaaaaa ctagcaatgc caaaggtaat ccaattgagg aagtaccaag gaagaagctg
     9421 taaatctagg acaaagtgct ggaacttgta gcccttcata aaggaacggc tagtttttag
     9481 gattcgtctt ggtgggacct gtcctaggtc tagactataa cagagaagaa attccacctg
     9541 tgaataggca taatactgtg gaatatagag gatatttcct acaatgatca agatgagact
     9601 tgcaagaaag tagagtccaa agaccatgag gaaacgctcg gtttcaactg atgagagatc
     9661 tagatttgga aactcaggat gtagggtgac gaattttttg gctaaaaagc tactataaaa
     9721 gaggaggtaa atcccaagta aattagggat actccataaa aagagataga aacgtttgag
     9781 aagtagggtc aaaaaggttt gagaaaagcg ctcctcatca aagagagcta ggctgttttt
     9841 tacagatggc tccgttttag aatctttcat gagtgtcagt gttgcataga cggaactggt
     9901 caaaagaata gtcccgataa aggagactag tagaggaaag aggtaggttt gaagtatttg
     9961 gccaagtatg ctgaaaaatg gctgttctaa aacagtcccg tggatccgag ataagggatt
    10021 aagaaaacca gataagatga ccagcatact gggaaggata tagaggagaa agagacgggg
    10081 ggtgtcagcc tgaaaatgtt ttgactcctg acgaattgtt tttaaatcaa tttttggata
    10141 gttcattctc ttattatacc atagttctta tacatagttc gtgacagttc ctactttttt
    10201 tgataaaatc atacagtgtg tccttgggca cactgtatga actgggactg tctttcccag
    10261 cttcggaggt aaaaaatgtc agattcacca atcaaatatc gtttgattaa gaaagaaaaa
    10321 cacacaggag ctcgtctagg agaaatcatc actccccacg gtacctttcc gacacctatg
    10381 tttatgccag ttgggacaca agccactgtc aaaactcagt cacctgaaga attgaaggag
    10441 atgggttcgg gaattatcct atcaaacacc tatcatctct ggcttcgccc tggagatgaa
    10501 ctcattgcac gcgctggtgg tctccacaag ttcatgaatt gggaccagcc tatcttgaca
    10561 gatagtggtg gttttcaggt ttattcttta gcagatagcc gtaatatcac agaagaagga
    10621 gtaaccttta aaaatcatct aaatggttct aagatgctcc tatccccaga aaaagccatc
    10681 tctattcaga ataatctggg ttcagacatc atgatgtcct ttgatgaatg tcctcagttt
    10741 tatcaacctt atgactacgt taagaaatcg atcgagcgta ccagccgttg ggctgagcgt
    10801 ggtttgaagg ctcaccgtcg tccacatgac caaggtttgt ttggaattgt gcaaggtgca
    10861 ggatttgaag accttcgccg ccaatcagct cacgatcttg tcagcatgga tttctcaggc
    10921 tactctatcg gtggtttggc agtgggagaa acccatgaag agatgaatgc ggtcttggac
    10981 tttacaactc aactgctgcc tgaaaataaa cctcgttatc tgatgggtgt gggagcgcca
    11041 gatagcttga tcgatggggt cattcgtggg gtggatatgt ttgactgtgt cttaccgact
    11101 cgaattgctc gtaacgggac ttgtatgacc agtcaaggac gtttggttgt gaaaaatgcc
    11161 cagtttgctg aggactttac gccactggat cctgagtgtg attgctacac atgtaataac
    11221 tatacacgcg cttaccttcg tcacctgctc aaggctgatg aaacctttgg tatccgcttg
    11281 actagctacc acaatcttta cttcttgctt aacctgatga agcaagtgcg acaagccatc
    11341 atggatgaca atctcttgga attccgtaag tattttgtgg aaaaatatgg ctataataag
    11401 tcaggacgta atttctaaaa tggaattgat ataagctaaa aatcctaagt tttctcttag
    11461 gatttttctt ctttttttga tagaataaag tgtacaatga aaggaagaat aaactcgtat
    11521 gcgcattaaa tggttttcct tgattaggat tataggttta cttttggtac tcttgtatca
    11581 cttctttcag acgatctttc ctggaggatt tttcggggta gatgtctttt tcacattttc
    11641 aggtttcctg attacagctc tactcattga agaattttct aagaacaatg agattgattt
    11701 gataggattt tttagaagac gcttttatcg gattgtgcca cctgtggttt tgatggtctt
    11761 ggtgaccatg ccttttactt tcttggttcg ccaagactat gttgctggaa ttggtggcca
    11821 gattgcgggc gtcttaggct ttatgaccaa cttctatgaa ctcttaacag gtgggagtta
    11881 tgaatctcag ttcattcctc atttgtttgt tcataattgg agtttggcag ttgaggttca
    11941 ctactatatt ctttggggat tggcagtttg gttcttatcc aaacaagcta aatcaaatgg
    12001 tcagttgaag gggatgatct ttctcttatc tgctactgcc ttcttgatca gtttcttctc
    12061 catgtttatt ggtagttttc tagtgacctc ttattcctct gtttatttct ccagtttaac
    12121 tcatgtctat ccattctttt tgggaagtat gttagcaact attgtaggcg ttcgtcagac
    12181 gacttccctc gtcaagcagt tggataaaat ctgggattta cgaaagactt tagtagtttt
    12241 tggaggaggc tttggtttcc tagttctttt gactttcttt gtcaaattca cttatctttt
    12301 tgcctatctt atcggcttct tgcttgccag tcttgcagct cttgccatga ttctggcggc
    12361 gcgtgtctta catgaaaaga cacatcatat acaggagccg aagattatca gctttttagc
    12421 ggatactagc tatgcggttt atcttttcca ttggcctttc tatatcattt tctcacagtt
    12481 gacatcaaat cttcttgctg tattactgac tctgatttgt tcttatggct ttgccagtct
    12541 gtcattttat gtattggaac cttggattgc aggcaagaac acacctattt tacaaactct
    12601 tcgtcccctg ccttatattc acgcaattct tgcagcaggt acaggaatct tgaccttcat
    12661 tgtctgcatg gtgaccttgt tggcaccaca agtgggagcg tttgagacag acttgactgt
    12721 caatggcttg aagcaagctg caacaaatat tggccagacc aaggtgatgg cagaacgggc
    12781 agatgcaaac agtttgggaa ttgctgatgg cactatgtta attggtgact cagtggcttt
    12841 aagggcaaat acagcactac agacagctct tcctggagca cagattaacg cgcaggtcag
    12901 cgtaacaacc aagaccgcaa atgaaatcat gctaaataat agccagaata aatttttacc
    12961 taagacggtg gtcattgcga ctggggtaaa taatcctgag aattacaagg atgactggga
    13021 cagtatcgtg aaaaatcttc ctaagggaca ccatatgatt ttggtgactc cttatgaggg
    13081 tgataagaca aaagagacct atgccatcgt tgagaaggct gctgcctata tgagagaatt
    13141 ggcagagaag acaccttaca ttacgatagc agattggaat caagttgcga aagagcatcc
    13201 agaaatttgg gctggaacag accaggttca tttcgggagt gagagtagca ctatcgaagc
    13261 aggagcaaaa ttgtatgcag atacgattgc cacagctttg cagacagctc aagacaagcc
    13321 ggttaaatca aaataacttg tattaaaaaa gagaagagtt ggagaaatcc agctctttta
    13381 aaatatctcc aaaaaatagg tctataccat ttacaaataa aaaagaaagg tttataatgt
    13441 aattgacata ataaattgta gaatcaatct tttaaggagg ttaacattat gcctaactat
    13501 attaaagcgg atcagttttt ctacccacac ggagttcgtc gaggtggtta cttggaactt
    13561 gtggacggca agtttggaaa acatgtagag cagattcctg aaggggctga ggtgattgac
    13621 tatacaggtt atagcattgc cccaggtctt gtggatactc atattcatgg atatgcaggt
    13681 gtagatgtga tggacaacaa cattgaaggt acattgcata ctatgagtga aggacttctt
    13741 agtaccggtg ttaccagttt cttacccaca actttaacag ccacttatga gcaattgctt
    13801 gcagtcactg aaaatcttgg aaaccattat aaagaagcaa caggtgctaa gattcgtggg
    13861 atttattatg aaggtccata tttcacagaa acttttaagg gggcacaaaa tccaacttat
    13921 atgagagacc cgggtgttga ggagtttcat tcttggcaaa aagcggcaaa tggcttgctt
    13981 aataaaattg cccttgcacc agaacgtgat ggggtggaag actttgttcg tacagttacg
    14041 ggcgaaggtg tgacggttgc tcttggacat tcaaacgcga cttttgatga agccaaaaaa
    14101 gcagtcgatg ctggagcgag tgtttgggtg catgcctaca atggaatgcg tgggttgact
    14161 caccgtgaat tgggtatggt tggagccatg taccaattgc cacataccta tgcagagttg
    14221 atctgtgatg gtcaccacgt agatccaaag gcttgcgaaa ttcttatcaa acaaaaagga
    14281 acagaaaaca tcgctcttat cacagactgt atgacagctg ggggattgga agacggagat
    14341 tatatgttgg gagaattccc agttgtcgtt gcaaatggaa ctgcacgcct caaatcgaca
    14401 ggtaacttgg caggttctat cctcaaactc aaagatggtt tgaagaatgt ggtcgaatgg
    14461 ggaattgcga atccgcatga agcagtcatg atggccagct tcaacccagc taaatccgtt
    14521 cacatcgatg acgtctgtgg ccaaatccgt gaaggctacg acgctgactt catcgtatta
    14581 gataaagatt tggaattggt agcaacctac ctagatggcg tgaaacgtta tcaagcataa
    14641 gagagaaggc agaagttaga gactaacttc tgctttttta tacagggtac tttactggta
    14701 aataaaaagt taaaaaaatc acaaaaaaag cttgaagaaa caaagaaaaa tgcttacaat
    14761 tgaactataa atattacaaa taaaaaaacg gaggagtgct ttatgaaagc ctatacttat
    14821 gttaaaccag gacttgcttc ttttgttgat gtagacaaac cagttattcg caagccaaca
    14881 gacgctattg tgcgtattgt aaaaaccact atttgtggaa cagacctcca tattatcaaa
    14941 ggggatgttc ctacttgcca aagtggtacc attcttggcc acgaagggat tgggattgtt
    15001 gaagaagttg gggaaggagt ttccaacttc aaaaaaggtg acaaggtctt gatttcttgc
    15061 gtctgtgcct gtggtaaatg ctactactgt aaaaaaggaa tttatgccca ctgtgaagac
    15121 gaagggggct ggattttcgg tcacttgatt gatggtatgc aggctgaata tctacgtgtc
    15181 cctcatgcag ataatactct ttaccatact ccagaagact tgtcagatga agctttggtt
    15241 atgctgtcag acattctgcc tactggatat gaaattggtg tcttaaaagg gaaagtagaa
    15301 cctggttgca gcgtagccat tattggttca ggtccagttg gattggctgc tcttttaaca
    15361 gcccaattct attcaccagc taaattgatt atggtagacc tagacgataa ccgcttggaa
    15421 actgccctat cattcggtgc gactcataag gttaattctt cagaccctaa aaaagccatt
    15481 aaagaaattt atgatttgac agatggtcgt ggtgtggatg tcgctatcga agctgtaggg
    15541 attcctgaaa catttgattt ctgtcaaaag attatcggtg tagacggaac ggttgccaac
    15601 tgtggtgtgc atggtaaacc agttgaattc gatttagata aactttggat tcgcaacatc
    15661 aatgtaacaa ctggtttggt atctacaaat acgactccac aattgttgaa agcacttgaa
    15721 agtcataaga ttgaaccgga aaaattggta actcactatt tcaaactcag tgaaattgaa
    15781 aaagcctacg aagtcttcag taaggcagca gaccaccatg ccattaaggt cattatcgaa
    15841 aacgatatct cagaagccta agtagtaaaa atatttttgt acataagcaa atagaaattc
    15901 agtcatccat cagatggctg gattttttat caaaaaatta agaaatgagc atatttcttt
    15961 ccttgtctgg cggaattggt tataatatac ggtacaaagg aatgaatgaa tatgtatcgt
    16021 gttatagaaa tgtacggaga ttttgaaccg tggtggttct tagaaggttg ggaagaagat
    16081 attgtagcaa gtagaaaatt tgaccagtat tatgatgctc tcaaatacta caaaacttgc
    16141 tggtttagat tggaacaaga atcccctctt tataaaagta gaagcgactt gatgaccatt
    16201 ttttgggacc cggaagacca acgctggtgt gatgaatgtg atgagtattt acaacaatac
    16261 cattctttgg ctcttttgca ggatgagcag gttatcccag acgaaaaact acgctcaggc
    16321 tatgaaaaac aaaccagtca ggaaaggaat cgttcttgcc gtatgaaatt aaaatagaga
    16381 aaagtaactt ttttggagtt gcttttttta tttttctaac gctttgcgaa tagtataggt
    16441 gaggaggtaa gtatggttca agaaattgca caagaaatca ttcgttcagc tcggaaaaaa
    16501 gggacgcagg atatttattt tgtccctaag ttagacgcct atgagcttca tatgagggta
    16561 ggagacgagc gctgtaaaat tggtagctat gattttgaaa agtttgcagc cgttatcagt
    16621 cactttaagt ttgtggcggg tatgaatgtg ggagaaaaaa gacgtagtca actgggttcc
    16681 tgtgattatg cctatgacca gaagatggcg tctctacgtt tatctactgt aggcgattat
    16741 cggggacatg agagtttggt tatccgtttg ttgcacgatg aggagcagga cttgcatttt
    16801 tggtttcagg atattgaaga attaggcaag cagtacaggc aacggggact ctatcttttt
    16861 gctggcccgg ttgggagtgg taagacgacc ttgatgcatg aattgtccaa gtcactcttt
    16921 aaaggacagc aagttatgtc catcgaagat cctgtcgaaa tcaagcagga cgacatgctt
    16981 cagttgcagt tgaacgaagc aatcggccta acctatgaaa atctaatcaa actttccttg
    17041 cgtcatcgac cagatctctt gattatcgga gaaattcgtg acagcgagac ggcgcgtgca
    17101 gtggtcagag ctagtttgac aggtgcgaca gtcttttcaa ccattcacgc caagagtatc
    17161 cgaggtgttt atgagcgtct gctggagttg ggtgtgagtg aagaagaatt ggcagttgtt
    17221 ctgcaaggag tctgctacca gagattaatc gggggaggag gaatcgttga ctttgcaaac
    17281 agagattatc aagaacacca agcagccaag tggaatgagc aaattgacca gcttcttaaa
    17341 gatggacata tcacaagtct tcaggctgag acggaaaaaa ttagctacag ctaagcaaaa
    17401 aaatatcatc accctattta acaatctctt ttctagcggt tttcatctgg tggagactat
    17461 ctccttttta gataggagtg ctttgttgga caagcagtgt gtgatccaga tgcgtgtggg
    17521 cttgtctcag gggaaatcat tctcagaaat gatggaaagt ttgggatgtt caagtgctat
    17581 tgtcactcag ttatccctag ctgaagttca tggcaatctc cacctgagtt tgggaaagat
    17641 agaagaatat ctggacaatc tggccaaggt caagaaaaaa ttgattgaag tagcgaccta
    17701 tcccttgatt ttgctgtttt ttcttctctt aatcatgctg gggctacgga attacctgct
    17761 cccacaactg gatagtagca atattgccac ccgaattatc ggtaatctgc cccaaatttt
    17821 tctaggcatg gcagggcttg tttccgtgct tgccctttta gcactaactt tttataaaag
    17881 aagttctaag atgaatgtct tttctatctt agcacgcctt ccctttattg gaatctttgt
    17941 gcagacctat ttgacagcct attatgcacg tgaatggggg aatatgattt cacagggaat
    18001 ggagttgacg cagatttttc aaatgatgca ggaacaaggt tcccagctct ttaaagaaat
    18061 cggtcaagat ctggctcaaa ccctgaaaaa tggccgtgaa ttttctcaga cgataggaac
    18121 ctatcctttc tttaggaagg aattgagtct catcatagag tatggggaag ttaagtccaa
    18181 gctgggtagt gagttggaaa tctatgctga aaaaacttgg gaagcctttt ttacccgagt
    18241 caaccgcacc atgaatttgg tgcagccact ggtttttatc tttgtggcac tgattatcgt
    18301 tttactttat gcggcaatgc tcatgcccat gtatcaaaat atggaggtaa atttttaaaa
    18361 tgaaaaaaat gatgacattc ttgaaaaaag ctaaggttaa agcttttaca ttggtggaga
    18421 tgttggtggt cttgctgatt atcagcgtgc ttttcttgct ctttgtacct aatctgacca
    18481 agcaaaaaga agcagtcaat gacaaaggaa aagcagctgt tgttaaggtg gtggaaagcc
    18541 aggcagaact ttatagcttg gaaaagaatg aagatgctag cctaagcaag ttaaaagacg
    18601 atgggcgaat cacggaagaa caggttaaag cttataaaga atacaatgat aaaaatgtag
    18661 gagcaaatcg taaagtcaat gattaaggcc tttaccatgc tggaaagtct cttggttttg
    18721 ggtcttgtga gtatccttgc cttgggcttg tccggctctg ttcagtccac ttttgcggca
    18781 gtagaggaac agattttctt tatggaattt gaagaactct atcgggaaac ccaaaaacgc
    18841 agtgtagcca gtcagcaaaa gactagtctg aacttagatg ggcagacgat tagcaatggc
    18901 agtcaaaagt tgccagtccc taaaggaatt caggtcccat caggccaaag tattacattt
    18961 gaccgtgctg ggggcaattc gtccctggct aaggttgaat ttcagaccag taaaggagcg
    19021 attcgctatc aattatatct aggaaatgga aaaattaaac gcattaagga aacaaaaaat
    19081 tagggcagtg attttactgg aagcagtagt cgctctagct atctttgcca gcattgcgac
    19141 cctccttttg ggacaaattc aaaaaaatag gcaagaggaa gcaaaaatct tgcaaaagga
    19201 agaagtcttg agggtagcta agatggcctt gcagacgggg caaaatcagg taagcatcaa
    19261 cggagttgag attcaggtat tttctagtga aaaaggattg gaggtctacc atggttcaga
    19321 acagttgttg gcaatcaaag agccataagg tcaaggcttt taccttgtta gaatccctgc
    19381 ttgccctcat tgtcatcagt gggggattac tcctttttca agctatgagt cagctcctca
    19441 tttcagaagt tcgctaccag caacaaagcg agcaaaagga gtggctcttg tttgtggacc
    19501 aacttgaggt agaattagac cgttcgcagt tcgaaaaagt agaaggcaat cgcctataca
    19561 tgaagcaaga tggcaaggac atcgccatcg gtaagtcaaa gtcagacgat tttcgtaaaa
    19621 ccgatgctag tggacggggg tatcagccta tggtttatgg cctcaaatca gctcagatta
    19681 cagaggacaa tcaactggtt cattttcgtt tccagtttca aaaaggctta gaaagggagt
    19741 tcatctatcg tgtggaaaaa gaagaaagtt aaggcaggtg ttctcctcta tgcagtcacc
    19801 atggcagcta tctttagtct tttgttgcaa ttttacttga accgacaagt cgcccactat
    19861 caagactatg ctttgaataa agaaaaattg gttgcttttg ccatggctaa acgaaccaag
    19921 gataaagccg agcaagaaag tggggaacag gtctttaatc taggtcaggt aagctatcaa
    19981 aacaagaaaa caagcttggt gacgactgtt cgtacgtcta agagccaata tgagtttctg
    20041 tttccttcag tcaaaatcaa agaagagaaa agagataaaa aggaagaggt agcgaccgat
    20101 tcaagcgaaa aagtggagaa gaaaaaatca gaagagaagc ctgaaaagaa agagaattcc
    20161 tagtcaattc aactataatg cgttgaatca agaatagtcc actatagttt ctagaaaatt
    20221 gctggaaatg gatgttaagc tccaattcat ttgtttatat cttatttcag tttactatac
    20281 tttgtgctaa attaaagata tgaaacatga ttttaaccac aaagcagaaa ctttcgattc
    20341 ccctaaaaat atcttcctcg caaacttggt atgtcaagca gccgagaaac agattgatct
    20401 tctatcagac aaagaaattt tagatttcgg tggtgacacg ggtctattag ccttgcccct
    20461 agcccctagc caagcaggct aagtcagtca ctcttgtaga catttctgag aaaatgttgg
    20521 agcaagctcg tttgaaagtg gagcagcaag caatcaagaa tatccagttt ttggagcaag
    20581 atttaccgaa aaatcccttg gagaaagagt ttgattgcct tgctgttagt cgggttcttc
    20641 atcatatgcc tgatttggat gcggctctct cactgtttca tcaacatttg aagtaagatg
    20701 ggaaactcat cattgctgat tttaccaaga cagaagctaa tcatcatgga tttgatttag
    20761 ctgaactgga aaacaagcta attgagcatg gtttttcatc tgtgcatagt cagattctct
    20821 atagcgctga agacttgttt caaggaaatc actcagaatt ctttttaata gtagcccaaa
    20881 aatcactcgc ctagtcaggg agtgattttt ctataaggat ggaaaaaaga agggaaattt
    20941 gataagatag gaatatggat tttgaaaaaa ttgaacaagc ttatacctat ttactagaga
    21001 atgtccaagt catccaaagt gatttggcga ccaactttta tgacgccttg gtggagcaaa
    21061 acagcatcta tctggatggt gaaactgagc taaaccaggt caaagacaac aatcaggccc
    21121 ttaagcattt agcactacgc aaagaagaat ggctcaagac ctaccagttt ctcttgatga
    21181 aggctgggca aacagaaccc ttgcaggcca atcaccagtt tacaccggat gctattgctt
    21241 tacttttggt gtttattgtg gaagagctgt ttaaagagga ggaaattact atcctcgaaa
    21301 tgggttctgg gatgggaatt ttgggcgcta ctttcttgac ctcgcttact aaaaaggtgg
    21361 attatttggg aatggaagtg gatgatttgc tgattgattt ggcagctagc atggcagatg
    21421 taattggttt gcaggctggc tttgtccaag gagatgccgt tcgtccacaa atgctcaaag
    21481 aaagtgatgt ggtcatcagc gacttgcctg ttggctatta tcctgatgat gccgttgcgt
    21541 cgcgccatca agttgcttct agtcaagaac atacttacgc ccatcacttg ctcatggaac
    21601 aaggacttaa gtacctcaag tcagacggat acgctatttt tctagctccg agtgatttgt
    21661 tgaccagtcc tcaaagtaat ttgttgaaag tctggttgaa agaggaggca agtctggttg
    21721 ctatgattag tttacctgaa aatctctttg ctaatgctaa acaatctaag actattttta
    21781 tcctacagaa gaaaagtgaa atagcagtag agccttttgt ttatccactt gctagcttgc
    21841 aagatgcaag tgttttaatg aaatttaaag aaaattttca aaaatggact caaggtactg
    21901 aaatataaaa tagattttgt tataatagtt gaaaacgctt aaaaaggggt atcatgttat
    21961 gacaaaaaca attgcaatca atgcaggaag ttcaagtttg aaatggcaat tatacttaat
    22021 gccagaagaa aaagtattgg cgaaaggttt gattgaacgt atcggtttga aagattcaat
    22081 ttcaactgta aaatttgacg gccgttctga acaacaaatt ttggatattg aaaatcatac
    22141 acaagctgtt aaaattttat tggatgactt gattcgtttc gatattatca aggcttatga
    22201 cgagattaca ggtgttggac atcgtgttgt tgctggtgga gaatatttca aagaatcaac
    22261 agttgttgag ggagatgttt tagaaaaagt tgaagagttg agtttgttgg ctcctctaca
    22321 caacccggca aatgcagcag gtgttcgtgc cttcaaggaa ttgttgccag acattaccag
    22381 tgtagttgtt tttgatactt ccttccacac aagtatgcca gagaaagctt atcgctaccc
    22441 tctaccaaca aaatattaca cagaaaacaa ggttcgtaaa tacggtgctc atggtacaag
    22501 tcaccagttt gtagcaggag aagctgcaaa actcttggga cgtccattag aagacttgaa
    22561 gttaattacc tgtcatattg gtaacggagg ctcaattaca gctgtgaaag ccggcaaatc
    22621 tgtagacact tctatggggt tcactcctct tggtggtatt atgatgggaa cgcgtacagg
    22681 ggatattgat ccagctatca ttccttattt aatgcaatat acagaggatt ttaacacacc
    22741 agaagatatc agtcgtgttc ttaaccgtga atcaggtctt ttgggagttt ctgctaattc
    22801 tagcgatatg cgcgatatag aagcagctgt agcagaaggg aatcacgagg ctagcttggc
    22861 ttatgaaatg tatgttgacc gtatccaaaa acatatcggt cagtaccttg cagtgctaaa
    22921 tggagcagat gccattgttt tcacagcagg tgtcggtgaa aatgcagaga gtttccgtcg
    22981 tgatgtaatc tcagggattt cgtggtttgg ttgtgatgtt gatgatgaaa agaatgtctt
    23041 tggcgttaca ggagacatct caacagaggc agctaaaatc cgtgtcttgg ttattccaac
    23101 agatgaagaa ttagtcattg cccgtgacgt tgaacgcttg aaaaaataag tgaaactaaa
    23161 aaaatattca atacaaggag ttgggaaagt tatttttcca gcttcttttt ctgatgaaat
    23221 tgtccaaaac cttgctatga ttggcttttt tgaaaaatat ggtataatag tagtaattta
    23281 atagatggag ttgagttttg aagaaaaact ttcgtgtaaa aagagagaaa gattttaagg
    23341 cgattttcaa ggaggggaca agttttgcta atcgcaaatt tgtggtctac caattagaaa
    23401 accagaaaaa ccattttcga gtaggtctat cagttagcaa aaaactgggg aatgccgtca
    23461 ctagaaatca aattaagcga cggattcggc atattatcca gaatgcaaaa gggagtctgg
    23521 tagaagatgt cgactttgtt gtcattgctc gaaaaggagt cgaaaccttg ggatacgcag
    23581 agatggagaa aaatctactc catgtattaa aattatcaaa gatttaccgg gaaggaaatg
    23641 ggagtgaaaa agaaactaaa gttgactagt ttgctaggac tgtctctgtt aatcatgaca
    23701 gcctgtgcga ctaatggggt aactagcgat attacagccg aatcggctga tttttggagt
    23761 aaattggttt acttctttgc ggaaatcatt cgctttttat cgtttgatat tagtatcgga
    23821 gtggggatta ttctctttac ggtcttgatt cgtacagtcc tcttgccagt ctttcaggtg
    23881 caaatggtgg cttctaggaa aatgcaggaa gctcagccac gcattaaggc gcttcgagaa
    23941 caatatccag gtcgagatat ggaaagcaga accaaactag agcaggaaat gcgtaaagta
    24001 tttaaagaaa tgggtgtcag acagtcagac tctctttggc cgattttgat tcagatgccg
    24061 gttattttgg ccctgttcca agccctatca agagttgact ttttaaagac aggtcatttc
    24121 ttatggatta accttggtag tgtggataca acccttgttc ttccgatttt agcagcagta
    24181 ttcacctttt taagtacttg gttgtccaac aaagctttgt ctgagcgaaa tggcgctacg
    24241 actgcgatga tgtatgggat tccagtcttg atttttatct ttgcagttta tgcgccaggt
    24301 ggagtcgccc tatactggac agtgtctaat gcttatcaag tcttgcaaac ctatttcttg
    24361 aataatccat tcaagattat cgcagagcgc gaggccgtag tacaggcaca aaaagatttg
    24421 gaaaatagaa aaagaaaagc caagaaaaag gctcagaaaa cgaaataaat aaggaggaat
    24481 ctggtaatgg tagtatttac aggttcaact gttgaagaag caatccagaa aggattgaaa
    24541 gaattagata ttccaagaat gaaggctcat atcaaagtca tttctaggga gaaaaaaggc
    24601 tttcttggtc tatttggtaa aaaaccagcc caagtggata ttgaagcgat tagtgaaacg
    24661 actgttgtca aagcaaatca acaggtagta aaaggcgttc cgaaaaaaat caatgatttg
    24721 aacgagcctg tgaagacggt tagtgaagaa accgttgacc ttggtcatgt ggttgatgct
    24781 attaaaaaaa tagaggaaga aggtcaaggt atttctgatg aagtcaaggc tgaaatctta
    24841 aaacatgaaa gacatgccag cactatctta gaagaaactg gtcacattga gattttaaat
    24901 gaacttcaaa tcgaggaagc gatgagggaa gaagcaggcg ctgatgacct tgaaactgag
    24961 caagatcaaa ctgaaaatca agacttgaaa gagatgggct tgaaggtcga gcaaagttat
    25021 gatattgccc aggtggctac ggatgtgact gcctatgttc aagcgattgt ggatgacatg
    25081 gatgttgaag ctacactttc aaatgattat aaccgtcgta gcatcaatct acaaattgac
    25141 accaacgaac caggtcgtat tatcggctac catggtaaag tcttgaaggc cttgcaactg
    25201 ttggctcaaa attatcttta caaccgctat tccagaacct tctacgttac aatcaatgtc
    25261 aatgattatg tcgaacaccg tgcagaagtc ttgcagacct atgcgcaaaa attggcgact
    25321 cgtgttttgg aagaagggcg cagtcataaa acagatccaa tgtcaaatag cgaacgcaag
    25381 attatccatc gtattatttc acgtatggat ggcgtgacta gttactctga aggtgatgag
    25441 ccaaatcgct atgttgttgt agatacagaa taagtaaaat caggtttatc ctgatttttt
    25501 gctagttaga ggaggttaaa ctgatgttga ataagataag agactattta gactttgctg
    25561 gtttgcagta ccgtaatcct gataaagcgg gagcagagcg agagaagatg ctggcattcc
    25621 gccacaaagg acaagaggcc cgaaaggctt ttacagaact ggccaaagcc tttcaagcaa
    25681 gccatccaga atggcaactc caacagacta gccagtggat gaatcaggcc cagcgtttga
    25741 gaccacattt ttgggtttat ctacagagag acggacaagt gacagaacct atgatggcct
    25801 tacgtttgta tgggacatct actgactttg gaatttcttt ggaagtcagt ttcatcgaac
    25861 gtaagaagga tgagcaaaca ctgggcaagc aggccaaagt tttagacatt ccaaccgtta
    25921 aagggattta ttatctaacc tactctaatg gtcaaagtca acggtgggag gcgaatgaag
    25981 aaaagcgtcg tactttacgc gagaaggtga gaagtcaaga agttcgaaaa gttttagtga
    26041 aggtagatgt tcctatgaca gaaaattcgt ctgaagaaga aatcgtagaa ggcttattga
    26101 agtcttattc taaaattctt ccctattatc tagctacgag aaaataagat aatttgtaaa
    26161 acatcataaa tcatacagtc caagagtgaa cagtccgctg tgtaattctt ggtctttttg
    26221 tttgcgcttt cgcattatat aataaactta caaaaacaat tcaaaaggag aacaattatg
    26281 gaagtcgttt caagtgttct aaattggttt tctagcaata ttttgcagaa tcccgcattt
    26341 ttcgtaggtt tattggtgtt gataggatat gcacttttga aaaaacctgc ccatgacgtt
    26401 ttttcagggt ttgttaaagc aacagtaggg tatatgttgc ttaacgtggg tgctggtggt
    26461 ttggttacaa cctttcgtcc aatcttagca gctcttaact acaaattcca aattggtgca
    26521 gcggttatcg acccttactt tggacttgct gcagcaaaca acaaaattgc agcagagttt
    26581 ccagattttg ttggaactgc aactacagct ctattgattg gttttggaat aaatatcttg
    26641 ctcgtagctc ttcgaaagat tacgaaggta agaaccctct ttattactgg tcacatcatg
    26701 gtacaacaag ctgcaacagt atctcttatg gttctattct tagtaccaca attgcgcaat
    26761 gcttacggta cagcagcgat tggtatcatc tgtggacttt actgggcagt tagttcaaat
    26821 atgactgttg aggcaactca acgcttgact ggtggtggcg gatttgcgat tggtcaccaa
    26881 cagcaatttg caatctggtt tgtagataaa gtagcaggac gctttggtaa gaaagaagaa
    26941 agtttagaca atcttaaatt acctaagttc ctctcaatct tccacgatac agttgttgca
    27001 tctgctacct tgatgctcgt attcttcgga gccattcttt taatcttggg tccagacatt
    27061 atgtctaata aagaagtcat cacttcagga actctattca atcctgctaa acaagatttc
    27121 tttatgtaca ttatccaaac agcctttacc ttctcagttt acttgttcgt tttgatgcaa
    27181 ggtgtccgaa tgttcgtatc tgagttgaca aacgccttcc aaggtatttc aaacaaattg
    27241 ttgccaggtt cattcccagc ggttgacgtt gcagcttctt atggatttgg ttctccaaat
    27301 gctgtcttgt caggatttac ctttggtttg attggtcaat tgattacaat tgtcttgctc
    27361 atcgtcttta aaaatccgat tcttattatt acaggatttg taccagtgtt ctttgacaat
    27421 gcagccattg cggtctacgc tgataaacgc ggcggatgga aagcggctgt tatcctttcc
    27481 tttatatcag gtgtccttca agttgctcta ggagctcttt gtgtggccct tctcgatttg
    27541 gcatcttatg gtggctacca tggaaatatc gactttgaat tcccatggct tggatttgga
    27601 tatatcttca aataccttgg tattgttggt tatgtacttg tgtgtctctt cttgcttgtt
    27661 attcctcaac ttcaatttgc caaagcaaaa gataaagaga aatattacaa cggtgaagtt
    27721 caagaagaag cttagtatct agaaaaggag aaataaaatg gttaaagtat tagcagcgtg
    27781 cggaaatgga atgggttcat caatggttat caagatgaag gttgaaaatg ctctccgtaa
    27841 gcttaatcaa acagatttta cagtcaattc atgcagtgtc ggtgaagcta aaggtttagc
    27901 agtaggatat gacatcgtaa tcgcttctct tcatttgatt caagaattgg aagggcgaac
    27961 taatgggaag ttaattggtc ttgataactt gatggatgat aaagaaatca ccgaaaaact
    28021 cagtcaagca ctacagtaaa aggttggagg gggctggaca gaaactgaga gttatcgttt
    28081 ctgtccttct ccctctttaa ataaaggagg cagatatgaa tttaaaacaa gctttaattg
    28141 acaatgactc gatccgacta ggtttagagg ctaacaattg gaaagaagca gtcaaggtag
    28201 cagtagatcc cctaattgaa agtggggcaa ttttgccaga gtattacgat gctatcattg
    28261 aatcgactga agagtatggg ccttactata tcttgatgcc aggtatggct atgccccacg
    28321 ctagacctga agctggtgtg caaagtgatg ccttttcatt gattacctta caaaatcctg
    28381 ttgtattttc agatgggaaa gaggtatctg ttttgttggc actagcagca acaagttcaa
    28441 aaattcacac aagtgtagcc attccacaaa ttattgccct gtttgaatta gaagattcta
    28501 ttgcacgttt acaggcttgc cagactaaag aagatgtctt ggctatgatt gaagaatcta
    28561 aggatagccc ttatctcgaa ggattggatt tggaaagtta gaaagaggaa taaagaaatg
    28621 acaaaaagaa tacctaattt acaagttgca ttagaccatt cagacttgca aggagcgatt
    28681 aaagcagctg tttctgttgg tcaggaagta gatattatcg aagctggaac tgtttgcttg
    28741 cttcaagttg gaagtgaact ggctgaagtc ttgcgtagcc ttttcccaga taagattatt
    28801 gtggcagaca caaaatgtgc tgatgctggt ggaacagttg ctaaaaataa tgcggttcgt
    28861 ggagcagact ggatgacttg tatctgttgt gcaaccatcc ctactatgga agcagctcta
    28921 aaggctatca agactgaacg aggagaacga ggcgaaatcc agatcgagct ttatggcgat
    28981 tggacttttg aacaagctca gctttggcta gatgcaggta tctcacaagc tatttatcac
    29041 caatctcgtg atgctcttct tgctggtgaa acttggggtg aaaaagacct taataaggtt
    29101 aaaaaactca ttgacatggg cttccgtgta tctgtaacag gtggtctaga tgtagatact
    29161 ctcaaactct ttgaaggtgt tgatgtcttt acctttatcg caggtcgtgg aattacagag
    29221 gctgcggatc cagcaggagc agcgcgtgcc ttcaaggatg aaatcaaacg aatttggggg
    29281 taaatcatgg tacgtccaat tggaatttat gaaaaggcaa ccccaacaca ctttacttgg
    29341 ctagaacgtt taaattttgc caaggagtta ggctttgatt ttgtcgagat gtctattgac
    29401 gaacgtgacg agcgtttagc aagacttgac tggagtaagg aagaacgctt ggaagttgtc
    29461 aaagcaatct atgaaactgg tgttcgtatt ccttctatct gtttttcagg ccatcgtcgc
    29521 tacccattgg gttcaaaaga tccagttcta gaggaaaaat ctctagaact catgaaaaaa
    29581 tgtatcgaat tagctcaaga cttgggagtt cgtacgattc aattagctgg ttacgatgtt
    29641 tactatgagg aaaagtcacc ccagacacgc caacgtttta tcaaaaattt gagaaaagcc
    29701 tgtgactggg ctgaagaagc tcaggtggta cttgctattg aaattatgga tgatcctttc
    29761 atcagtagca tcgaaaaata tttggctata gaaaaagaga ttgactctcc cttcctcttt
    29821 gtatatccag atattggtaa tgtgtctgca tggcataatg atatctatag tgagttttat
    29881 cttggtcatc atgccatcgc agctctccat ctcaaggata cttatgcagt gacagaaagt
    29941 tcaaagggcc agttccgaga tgtacctttc gggcaaggtt gtgtcaaatg ggaagaagct
    30001 ttcgatattt taaaggaaac caattataat ggacctttcc taatcgaaat gtggtctgaa
    30061 aattgtgaaa cagtagaaga aacacgcgca gccgttcaag aggcgcaagc ttttctctat
    30121 ccactcatta agaaagcagg tttgatgtaa gatgaatcaa gtaatcaatg ctatgcgtaa
    30181 acgagtctgt gatgccaatc aatcattgcc aaaacatgga cttgtcaaat ttacctgggg
    30241 gaatgtatct gaagttaatc gcgaactcgg tgtcattgtt atcaaaccat caggcgtgga
    30301 ttatgacgaa ttgacacctg aaaacatggt agtgactgat ctagatggta agatcctaga
    30361 aggggattta agaccatctt ccgacctccc aactcatgtg caattatata aggcttggtc
    30421 agaaattggt agtgtggttc acacccattc gacagaagct gttggttggg ctcaggcagg
    30481 tcgtgatatt cctttctacg gaacaaccca tgcagattat ttctacggtt caatcccttg
    30541 cgcccgtagt ttgaccaagg acgaagtaga agtggcctat gaaaaagata ctggcctggt
    30601 tatcgtagaa gagtttgaac atcgcggact taacccggtt gaagtaccag gaattgttgt
    30661 acgcaatcac ggtccattca cctggggcaa aaatccagag aatgctgttt atcactctgt
    30721 cgtactagag gaagtatcaa agatgaatcg ctttacagaa caaatcaatc caagagttgg
    30781 acctgctccc cagtacatac tagaaaaaca ctaccaacgt aaacatggac caaatgctta
    30841 ttatggtcaa aagtaagaac gatgaaggag gagaaaaaga taaatttagc tcctcttttt
    30901 acatttgatt tttattgaga gtaaagttgg aattgaagta attttaaaag attttttaga
    30961 aatagcgctt gatatatata tggtaaaata aaaagaattg ctgtgatatc aatagatttg
    31021 ggggattttt taatatggta ctggataagg caagttgtga tttgcttcaa tatttgatgg
    31081 atcaagaaac gtccaaaacg attatggcga tttcgaaaga tttgaaagag tcaagaagga
    31141 aaatttatta tcacattgac aaaatcaatg ctgctctggg tgacgaggcg cttcacatca
    31201 ttagtattcc acgaattggt attcacttaa cggaagagca gagagatgct tgttgtaaac
    31261 tattatcgga agtagattcg tacgattata tcatgagtgc gcatgaacgt atgatgataa
    31321 tgttactatg gataggtatt tctaaagaac gtattacgat tgaaaaattg atagagttaa
    31381 cagaggtatc taggaatact gttctcaatg atttgaatag tattcgttat caactaactt
    31441 tggaacaata tcaggtgatc ttgcaagtga gcaagtcaca gggataccac cttcatgccc
    31501 accctcttaa taaaattcag tatcttcaat cgcttctata tcatattttt atggaagaaa
    31561 atgccacttt tgtatctatt ttagaagata agatgaaaga gaggttagat gatgagtgtt
    31621 tgctttctgt tgaaatgaac caatttttta aggaacaggt tcctttagtt gaacaagatt
    31681 tagggaagaa aataaaccat catgaaataa cttttatgtt gcaggttcta ccttatttgc
    31741 tgttaagctg tcataatgtt gaacagtatc aagaaagaca tcaggatata gagaaagaat
    31801 tttctttgat aagaaaaaga atagagtatc aggtgtctaa gaaattagga gaacggttgt
    31861 ttcaaaagtt tgaaatttct ttgtcaggac ttgaagtttc tcttgtagct gttctcctcc
    31921 tctcctatcg taaagatttg gatattcatg cagaaagtga tgattttcgg caattaaaac
    31981 ttgctttaga agaatttatc tggtattttg aatcacaaat ccgaatggag attgagaaca
    32041 aggatgattt gttacgaaat ttgatgatcc actgtaaagc cttgttattt agaaagactt
    32101 acggtatttt ttctaaaaat cctctaacaa aacaaattcg atccaagtat ggagaattat
    32161 ttttagtcac tagaaaatct gcggaaattt tagaaggagc atggtttatt cggctaacag
    32221 acgatgatat tgcctatttg acgattcata ttggaggatt tttaaaatat acaccatcgt
    32281 ctcaaaaaaa tatgaaaaaa gtttatctcg tttgtgatga aggtgttgcg gtttcgagac
    32341 ttttgctgaa acaatgcaaa ctttattttc caaatgagca aattgacact gtatttacaa
    32401 cagaacaatt taagagtgtg gaagatattg cacaagttga tgtagtgatt actactaatg
    32461 atgatttgga tagcagattt ccgattttaa gggttaatcc tatccttgaa gcagaagata
    32521 ttttgaaaat gctagactat cttaaacaca atatatttcg taataagagc aaaagtttca
    32581 gtgaaaatct ttctagtctt atttcgtctt atattgtaga tagcaagttg gctagtaagt
    32641 tccaagaaga ggttcaaaca cttataaatc aagaaatagt agttcaagct tttttggaag
    32701 atatttgaag gacagtccaa tgatgaacac aaacctgtgt ttttcttggt cttttttagt
    32761 gttttgaagg gtggtatact aatctcaaag ataacaatta tatccaaagg aggcaacata
    32821 tgccaaacgt caaagaaatt acaagagagt catggatttt agccactttc ccagagtggg
    32881 gaacatggtt gaacgaagaa atcgaagaag aagtcgtacc tgaaggcaac tttgccatgt
    32941 ggtggctagg caactgtggt acttggatta agacaccagc tggtgctaac gttgtcatgg
    33001 acctttggtc aaaccgtgga aaatcaacca aaaaagtgaa agatatggtt cgtgggcacc
    33061 aaatggcaaa tatggcaggt gttcgtaagt tgcaaccaaa cttgcgtgtt cagccaatgg
    33121 ttatcgatcc atttgctatc aacgaactag actattactt agtttcacac ttccacagtg
    33181 atcatatcga cccatacaca gctgcagcaa ttctcaacaa tcctaagtta gagcatgtta
    33241 agtttatcgg tccttaccac tgtggacgaa tctgggaagg atggggtgtt ccaaaagaac
    33301 gtatcatcgt tgttaaacca ggtgacacta tcgaattaaa agatatgaag attcatgcag
    33361 tagaatcatt tgaccgtact tgcttggtaa ctctcccagt gaacggtgct gatgagacag
    33421 gcggtgaact tgctggcttg gctgttacag atgaagaaat ggctcaaaag gctgttaact
    33481 atatctttga aacaccaggt ggaaccatct atcatggtgc agattctcac ttctcaaact
    33541 attttgcaaa acatggtaaa gactttaaaa ttgatgttgc tttgaataac tatggtgaaa
    33601 atccggtagg tatccaagac aaaatgacat ctatcgacct tcttcgtatg gcagaaaatc
    33661 tgcgtaccaa agtcattatc ccagttcact atgatatctg gtctaacttc atggcttcta
    33721 ctaatgagat tctagaactt tggaaaatgc gaaaagatcg cttgcaatac gatttccatc
    33781 catttatctg ggaagttggc ggtaagtaca cttatcctca agatcaacac ttagtagaat
    33841 accatcatcc acgtggtttt gatgattgtt ttgaacaaga ctctaacatt caatttaaag
    33901 ctttgctata atataaaaat attctttgga ggttatccgt atgttgcatg atacggataa
    33961 ttttcatata atagtaaaat agaatgtgtg attcaataat cacctcaaat agaaaggaaa
    34021 ttctatgtca aatctatctg ttaatgcaat tcgttttcta ggtattgacg ccattaataa
    34081 agccaactca ggtcatccag gtgtggttat gggagcggct ccgatggctt acagcctctt
    34141 tacaaaacaa cttcatatca atccagctca accaaactgg attaaccgcg accgctttat
    34201 tctttcagca ggtcatggtt caatgctcct ttatgctctt cttcaccttt ctggttttga
    34261 agatgtcagc atggatgaga ttaagagttt ccgtcaatgg ggttcaaaaa caccaggtca
    34321 cccagaattt ggtcatacgg cagggattga tgctacgaca ggtcctctag ggcaagggat
    34381 ttcaactgct actggttttg cccaagcaga acgtttcttg gcagccaaat ataaccgtga
    34441 aggctacaat atctttgacc actatactta cgttatctgt ggagacggag acttgatgga
    34501 aggtgtctca agcgaggcag cttcatacgc aggtttgcaa aaacttgata agttggttgt
    34561 tctttatgat tcaaatgata tcaacttgga tggtgagaca aaggattcct ttacagaaag
    34621 tgttcgtgac cgttacaatg cctacggttg gcatactgcc ttggttgaaa atggaacaga
    34681 cttggaagcc atccatgctg ctatcgaaac agcaaaagct tcaggcaagc catctttgat
    34741 tgaagtgaag acggttattg gatacggttc tccaaacaaa caaggaacta atgctgtaca
    34801 cggcgcccct cttggagcag atgaaactgc atcaactcgt caagccctcg gttgggacta
    34861 cgaaccattt gaaattccag aacaagtata tgctgatttc aaagaacatg ttgcagaccg
    34921 tggcgcatca gcttatcaag cttggactaa attagttgca gattataaag aagctcatcc
    34981 agaactggct gcagaagtag aagccatcat cgacggacgt gatccagtcg aagtgactcc
    35041 agcagacttc ccagctttag aaaatggttt ttctcaagca actcgtaatt caagtcaaga
    35101 tgccttgaat gttgtggcag ctaaattacc aaccttccta ggtggatcag ctgaccttgc
    35161 tcactcaaac atgacttata tcaagacgga tggacttcaa gacgatacca atcgcttgaa
    35221 tcgtaacatt cagtttggtg ttcgtgaatt tgcaatggga acaatcttga acgggatggc
    35281 ccttcatggt ggacttcgtg tatacggtgg aactttcttc gtcttctctg actatgtgaa
    35341 ggcagctgtc cgcttgtcag ccttacaagg acttcctgtg acttatgtct ttacccatga
    35401 ttcaatcgca gttggggaag atggtccgac tcacgaacca gttgagcatt tagcaggtct
    35461 tcgtgctatg ccaaatctaa atgttttccg tccagcagat gcgcgtgaaa cgcaagcagc
    35521 ttggtacctt gcagtgacaa gtgagaaaac accaactgcc cttgtcttga cacgtcaaaa
    35581 cttgactgtt gaagatggaa cagacttcga caaggttgct aaaggtgctt atgttgtata
    35641 tgaaaatgca gccgactttg ataccatctt gattgcgaca ggttcagagg ttaatcttgc
    35701 tgtctcagct gctaaagaat tggctagtca aggcgaaaaa atccgcgtag tcagcatgcc
    35761 atctacagat gtctttgata aacaagatgc agcttacaag gaagaaattc ttccaaatgc
    35821 agtccgccgt cgtgttgcag tcgaaatggg tgcaagtcaa aactggtaca aatatgttgg
    35881 tctcgatggt gccgttctag gtattgatac cttcggagcc tctgccccag caccaaaagt
    35941 attggcagaa tatggcttta ctgtcgaaaa tcttgtaaaa gttgttcgaa atttgaaata
    36001 atcctaaaaa tcagggcgta agctctggtt tttcttacca gaaaagtaag gtacaatctt
    36061 gtaaaagtag ctgaaatttg atatagtagt cctatgtaaa agacaaagga gaaaacaatg
    36121 aatccaaata ttactttttt aatcatgctt gtaggtatga tggccttgat gttctttatg
    36181 caacgttctc aaaagaaaca agctcaaaag cgtatggaaa gcttaaataa actacaaaaa
    36241 ggctatgaag tgattacaat cggtggactt tatggaacag tcgatgaagt agatacggag
    36301 aagggaacaa tagttcttga cgtagatgga gtttacttga cttttgaact agctgctatc
    36361 aagacagtat taccgctgaa agaaacagct tcactagaag gcgcaattga aaaataagac
    36421 gggatcacga actcccgttt tctataaaag aaaggaaatg ggatgaaaaa attagtcttt
    36481 gtctgtctgg gaaatatttg ccgtagccct atggccgagt ttgttatgaa atcaatgaca
    36541 gataactacg aaatccaaag tcgagcaact tcctcttggg aacatggcaa tccgattcat
    36601 aaggggactc agggaatttt tcaagagtat gagattcctt atgacaagaa caagacatcg
    36661 cttcagatta gtaaggaaga ttttgaagcc tttgattata ttatcggaat ggacgcttca
    36721 aatatttctg acttacgtca gatgtgtcca gtagactgtc aagataagat ttactcattt
    36781 tcatctgaaa gtgttccaga cccttggtat acaggagatt ttgaagaaac ctatcgacgt
    36841 gttcaagagg gctgtcaagc ttggttagaa cgtttagaaa aggagagtta aggtggaaaa
    36901 tcttacgaat ttttacgaaa agtatcgtgt ctatctgact cgtccacgtt tagagctttt
    36961 ggcagtagtt accattgttt tctgtgctgt actcgtcttt tttctaaata ttccaggaaa
    37021 aggtgtctta aaactcgata atggaacgat tgtttatgat ggcagtcttg tccgtggtaa
    37081 aatgaatggc caaggtacca ttaccttcca aaatggagac caatatacag gtggcttcaa
    37141 caatggagcc ttcaacggaa aaggtacctt tcaatctaaa gaaggctgga cctacgaagg
    37201 tgattttgta aatggtcagg ctgaaggaaa agggaaacta acaacagaac aagaagtcgt
    37261 ttatgaagga acttttaaac aaggcgtttt tcaacaaaaa taaagcctcc tatcaaagga
    37321 ggtattatta gaatcacaag gtaagcgctt acctgtgaat ccctttcttt ccaaatccct
    37381 cttccaagca agtttgtgaa ataaaaaata tttgaaataa atttcacaaa cttcaaagat
    37441 aaaacttgat aagaaaagaa aatgagaaaa gtttcacaag agtttaaaaa ttctttgtga
    37501 aatgtttatg aaactgttta caaagttgaa ataatgcgtt ataataaact tgtgaaaaaa
    37561 ttaacaaagg atatcgttcc ttgaaagcta tggaggaaaa tatggctgat aaaaaaactg
    37621 tgacaccaga ggaaaagaaa ctcgttgctg aaaaacacgt agatgagttg gttcaaaaag
    37681 ctctagttgc ccttgaagaa atgcgtaaat tggatcaaga acaagttgac tacatcgttg
    37741 ccaaagcatc agtagcagct ttggatgccc acggagaatt ggctttacat gcctttgaag
    37801 aaacaggacg tggtgtattt gaagacaaag caactaagaa cttgtttgct tgtgaacacg
    37861 tagtaaacaa catgcgccac actaagacag ttggcgttat cgaagaagac gatgtaacag
    37921 gattgactct tattgctgaa ccagttggtg ttgtttgtgg tattactcca acaacaaacc
    37981 caacatcaac agcaatcttc aaatcattga tttcattgaa gacacgtaac ccaatcgtct
    38041 ttgccttcca tccatcagca caagaatcat ctgctcatgc agctcgtatc gtccgcgatg
    38101 cagctatcgc agctggtgct cctgaaaact gtgtacagtg gatcactcaa ccatctatgg
    38161 aagcaactag tgcccttatg aaccacgaag gtattgcaac aatccttgca acaggtggta
    38221 atgccatggt taaagccgca tactcatgtg gtaaaccagc tcttggggta ggtgccggaa
    38281 acgttccagc ttatgttgaa aaatcagcaa acattcgtca agcagcacac gatatcgtca
    38341 tgtctaaatc atttgataac ggtatggtct gtgcatctga acaagcagtt atcattgata
    38401 aagaaattta cgatgaattt gtagcagagt tcaaatctta ccacacttac tttgtaaaca
    38461 aaaaagaaaa agctcttctt gaagagttct gcttcggcgt caaagcaaac agcaaaaact
    38521 gtgctggtgc aaaattgaac gctgacatcg ttggtaaacc agcaacttgg attgcagaac
    38581 aagcaggatt tacagttcca gaaggaacaa acattcttgc tgcagaatgt aaagaagttg
    38641 gcgaaaatga gccattgact cgtgaaaaat tgtcaccagt tattgcagtt ttgaaatctg
    38701 aaagccgtga agatggtatt actaaggctc gtcaaatggt tgaatttaac ggtcttggac
    38761 actcagcagc tatccacaca gctgacgaag aattgactaa agaatttggt aaagctgtta
    38821 aagctattcg tgttatctgt aactcacctt ctacttttgg tggtatcggg gacgtttaca
    38881 atgccttctt gccatcattg acacttggat gtggttctta cggacgcaac tcagttgggg
    38941 ataacgttag tgccattaac ctcttgaata tcaaaaaagt cggaagacgg agaaataaca
    39001 tgcaatggat gaaacttcct tcaaaaacat actttgaacg tgattcaatt caataccttc
    39061 aaaaatgtcg tgacgttgaa cgtgtcatga tcgttactga ccatgccatg gtagagcttg
    39121 gtttccttga tcgtatcatc gaacaactgg accttcgtcg caataaggtt gtttaccaaa
    39181 tctttgcgga tgtagaaccg gatccagata tcacaactgt aaaccgtggt actgagatta
    39241 tgcgtgcctt caaaccagat accatcatcg cactcggtgg tgggtctcca atggatgctg
    39301 ccaaagtaat gtggctcttc tacgagcaac cagaagtgga cttccgtgac cttgtccaaa
    39361 aattcatgga tatccgtaaa cgtgccttca agttcccatt gcttggtaag aagactaaat
    39421 tcatcgcgat tccaactaca tctggtacag gatctgaagt aacaccattt gccgttatct
    39481 ctgataaagc aaacaaccgt aaatacccaa tcgctgacta ctcattgaca ccaactgtgg
    39541 caatcgtaga tcctgctttg gtattgacag ttccaggatt tgttgctgct gatactggta
    39601 tggacgtatt gactcacgcg acagaagcat acgtatcaca aatggctagt gactacactg
    39661 atggtttagc acttcaagcc attaaattgg tctttgaaaa tctcgaaagc tcagttaaga
    39721 atgcagactt ccactcacgt gagaaaatgc ataacgcttc aacaatcgct ggtatggcct
    39781 ttgccaatgc cttcctaggt atttctcact caatggccca taagattggt gcgcaattcc
    39841 acacaatcca cggtcgtaca aatgctatct tgcttccata cgttatccgt tacaacggta
    39901 cacgtccagc taagacagca acatggccta agtacaacta ctaccgtgca gatgaaaaat
    39961 accaagatat cgcacgcatg cttggacttc cagcttctac tccagaagaa ggggttgaat
    40021 cttacgcaaa agctgtctac gaactcggtg aacgtattgg gatccaaatg aattttagag
    40081 accaaggaat tgacgaaaaa gaatggaaag aacattctcg tgaattagcc ttcctggctt
    40141 atgaagacca atgttcacca gctaacccac gtcttccaat ggtagaccat atgcaagaaa
    40201 tcatcgaaga tgcatactat ggctacaaag aaagaccagg acgccgtaaa taattgttta
    40261 tcagtctaga agcaagacaa aaactcaatt tgagggaaag atccagtact ttttctatga
    40321 taaaaggcat cctatcaagg tttttgaaca cctgatagga tgccttttta tgatattgag
    40381 gcctttttgc cctttttgaa aaactagaat agaaacaaaa tatataatag attgaaactg
    40441 gaatagtaca cttctgcttc taaaacattg ttagaattcg atttgactgt cctgatcgat
    40501 ttgtcctgtt cttatttcat tttaatatat aaaaaatat
//
LOCUS       DAVBNO010000016        35482 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVBNO010000016 DAVBNO010000000
VERSION     DAVBNO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.016"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..270
                     /locus_tag="VQ846_000905"
     CDS             <1..270
                     /locus_tag="VQ846_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3075499.1"
                     /translation="QHDSYHRFLESKGIQASMSRKGNSQDNGMMESFFGILKSEMFYG
                     YEKSFQSLKQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(315..956)
                     /gene="plsY"
                     /locus_tag="VQ846_000906"
     CDS             complement(315..956)
                     /gene="plsY"
                     /locus_tag="VQ846_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET3075500.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            1092..3035
                     /gene="parE"
                     /locus_tag="VQ846_000907"
     CDS             1092..3035
                     /gene="parE"
                     /locus_tag="VQ846_000907"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET3075501.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            3455..5926
                     /gene="parC"
                     /locus_tag="VQ846_000908"
     CDS             3455..5926
                     /gene="parC"
                     /locus_tag="VQ846_000908"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET3075502.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            6058..7080
                     /locus_tag="VQ846_000909"
     CDS             6058..7080
                     /locus_tag="VQ846_000909"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET3075503.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            7136..7840
                     /locus_tag="VQ846_000910"
     CDS             7136..7840
                     /locus_tag="VQ846_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET3075504.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNGTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            7905..8594
                     /locus_tag="VQ846_000911"
     CDS             7905..8594
                     /locus_tag="VQ846_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET3075505.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            8591..9514
                     /locus_tag="VQ846_000912"
     CDS             8591..9514
                     /locus_tag="VQ846_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET3075506.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            9529..10173
                     /gene="pcp"
                     /locus_tag="VQ846_000913"
     CDS             9529..10173
                     /gene="pcp"
                     /locus_tag="VQ846_000913"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET3075507.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            10341..10571
                     /locus_tag="VQ846_000914"
     CDS             10341..10571
                     /locus_tag="VQ846_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET3075508.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            10621..10701
                     /locus_tag="VQ846_000915"
     tRNA            10621..10701
                     /locus_tag="VQ846_000915"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10655..10657,aa:Tyr,seq:gta)
     gene            10711..10782
                     /locus_tag="VQ846_000916"
     tRNA            10711..10782
                     /locus_tag="VQ846_000916"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10743..10745,aa:Gln,seq:ttg)
     gene            10944..12146
                     /gene="rpsA"
                     /locus_tag="VQ846_000917"
     CDS             10944..12146
                     /gene="rpsA"
                     /locus_tag="VQ846_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET3075509.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            12232..12729
                     /locus_tag="VQ846_000918"
     CDS             12232..12729
                     /locus_tag="VQ846_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET3075510.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            12729..14384
                     /gene="dnaX"
                     /locus_tag="VQ846_000919"
     CDS             12729..14384
                     /gene="dnaX"
                     /locus_tag="VQ846_000919"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET3075511.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVNLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            14412..14606
                     /locus_tag="VQ846_000920"
     CDS             14412..14606
                     /locus_tag="VQ846_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET3075512.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            14756..15526
                     /gene="sufC"
                     /locus_tag="VQ846_000921"
     CDS             14756..15526
                     /gene="sufC"
                     /locus_tag="VQ846_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET3075513.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            15554..16816
                     /gene="sufD"
                     /locus_tag="VQ846_000922"
     CDS             15554..16816
                     /gene="sufD"
                     /locus_tag="VQ846_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET3075514.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIAHSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSYADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            16827..18053
                     /locus_tag="VQ846_000923"
     CDS             16827..18053
                     /locus_tag="VQ846_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET3075515.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVSATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            18040..18480
                     /locus_tag="VQ846_000924"
     CDS             18040..18480
                     /locus_tag="VQ846_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET3075516.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            18534..19946
                     /gene="sufB"
                     /locus_tag="VQ846_000925"
     CDS             18534..19946
                     /gene="sufB"
                     /locus_tag="VQ846_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET3075517.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFNQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(20145..21386)
                     /locus_tag="VQ846_000926"
     CDS             complement(20145..21386)
                     /locus_tag="VQ846_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET3075518.1"
                     /translation="MKKIFLTLLTVSLLGGVSTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            21489..22598
                     /locus_tag="VQ846_000927"
     CDS             21489..22598
                     /locus_tag="VQ846_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET3075519.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            23244..23591
                     /gene="ssrA"
                     /locus_tag="VQ846_000928"
     tmRNA           23244..23591
                     /gene="ssrA"
                     /locus_tag="VQ846_000928"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(23774..23861)
                     /locus_tag="VQ846_000929"
     tRNA            complement(23774..23861)
                     /locus_tag="VQ846_000929"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23825..23827),aa:Ser,seq:gga)
     gene            24023..24763
                     /locus_tag="VQ846_000930"
     CDS             24023..24763
                     /locus_tag="VQ846_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET3075520.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            24760..25671
                     /gene="pfkB"
                     /locus_tag="VQ846_000931"
     CDS             24760..25671
                     /gene="pfkB"
                     /locus_tag="VQ846_000931"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET3075521.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            25668..27620
                     /locus_tag="VQ846_000932"
     CDS             25668..27620
                     /locus_tag="VQ846_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET3075522.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTEHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            27784..30009
                     /locus_tag="VQ846_000933"
     CDS             27784..30009
                     /locus_tag="VQ846_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET3075523.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(30162..30590)
                     /locus_tag="VQ846_000934"
     CDS             complement(30162..30590)
                     /locus_tag="VQ846_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075524.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            30728..31870
                     /locus_tag="VQ846_000935"
     CDS             30728..31870
                     /locus_tag="VQ846_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET3075525.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            31879..33093
                     /gene="thiI"
                     /locus_tag="VQ846_000936"
     CDS             31879..33093
                     /gene="thiI"
                     /locus_tag="VQ846_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET3075526.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            33228..34052
                     /locus_tag="VQ846_000937"
     CDS             33228..34052
                     /locus_tag="VQ846_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET3075527.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            34065..35231
                     /locus_tag="VQ846_000938"
     CDS             34065..35231
                     /locus_tag="VQ846_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET3075528.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
BASE COUNT        10614 a         6514 c         7911 g        10443 t
ORIGIN      
        1 caacacgatt cttatcatcg gttcttagag agtaagggaa ttcaagcatc catgtcacgc
       61 aagggaaaca gccaagacaa tggtatgatg gagtccttct ttgggattct gaaatcggaa
      121 atgttttacg gttatgagaa gtcgtttcag tcgcttaagc aattggaaca agccattata
      181 gactatattg attactacaa caacaagaga attaaggtaa aactaaaagg acttagccct
      241 gtgcaataca gaactaaatc cttcggataa attaattgtc taacttttgg ggggcagttc
      301 acagaactgg cattttattt tttaggatct tgatgggtta ggttcaatcc ccaagggacc
      361 aaattttcag ttttattttt gatacgagct atattgtcct tatgacgaat gataatcaaa
      421 ctagcaagtg ctaagataat agcgatgaag agagagtcat agttactcag gataaaacca
      481 aaaagtggaa agagcagaac cccgataaca gccgcgatcg atgctgtgac actagacagt
      541 gaaatcatac tgccaagata gagagctcca aagaagataa tcgcaaggta gagacagaag
      601 ataggcgcaa atccgaaaat tactccagca ctggttgcga cagccttacc acctttaaat
      661 cctgcaaaga tagggaaggt atggccgata acagccaaaa gtccaaagat gagaggagaa
      721 acgccttgta gatgaaaaat aatcggaagc agcgttgcta gggttccttt gaaaaagtca
      781 atcacaaagg ttgccatacc agctttctta cctaaaatgc ggaaggtatt ggtcgttcca
      841 gtgttaccag aaccatgctc gcgtagattg atttgaaaga atacttgtcc aatccagaga
      901 ccagatggaa tcgaacccag cagataggct aggattaata aaactattgt aatcatactc
      961 ctattatatc atgaattggg aaagaaaagc agagaatctc tgctaaaatt gtcacatcgg
     1021 agaaagaaaa attttgcaaa atccttggaa aacctgtaga atagtaaaga tgaacgaata
     1081 ggaggttcct tgtgtcaaaa aaggaaatca atattaacaa ttataatgat gatgctattc
     1141 aggtgctaga agggttggat gcggtccgaa aacgtccagg gatgtatatt ggatcgaccg
     1201 atggcgctgg tcttcatcac ctagtttggg aaatcgttga taatgcagtc gatgaagcct
     1261 tgtctgggtt tggtgatcgt atcgatgtaa ccatcaataa agacggtagt ctaacggttc
     1321 aagaccatgg acgtgggatg ccgacaggta tgcacgctat gggaattcca actgttgagg
     1381 ttatctttac cattcttcat gccggaggga aattcggtca aggtggctat aagacatcag
     1441 gtggacttca cggagtgggt tcttccgttg ttaacgccct ttctagctgg ttagaagttg
     1501 aaattacccg tgatggcgca gtttacaagc aacgtttcga aaatggtgga aaacctgtca
     1561 cgactttgaa gaaaatcggt acagcaccca agtctaaaac aggcaccaaa gttactttta
     1621 tgcctgacgc gactatattt tctacgacag atttcaagta caataccatt tcagagcgcc
     1681 ttaatgaatc agcctttctc ttgaaaaatg tgaccttgtc tttaacggac aagcgaacag
     1741 atgaagcgat tgagttccac tatgagaatg gagtacaaga ttttgtttct tatctcaacg
     1801 aagataagga aatcttgacg ccagttcttt actttgaagg ggaagacaat ggttttcaag
     1861 tggaagtagc cctccagtac aatgacggat tctcagataa cattctatcc tttgtcaata
     1921 acgttcgcac caaggacggt ggaacgcacg agacaggact caagtctgcc attaccaagg
     1981 tcatgaatga ctatgcacgt aaaacaggtc ttctcaagga aaaagataaa aaccttgaag
     2041 gttcagacta tcgtgaggga ctagcggccg ttctttctat cttagttcct gaagaacact
     2101 tgcagtttga aggacagacc aaggataaac taggaagccc cctagctcgc ccagttgtgg
     2161 atggaatagt ggctgataag ttgacctttt tccttatgga aaatggggaa ttagcttcta
     2221 acctcatccg caaggctatc aaggcccgtg atgctcgtga agcagcacgt aaggcgcgtg
     2281 atgagagccg aaatgggaag aaaaacaaga aagataaggg cttgttgtct gggaaattga
     2341 ccccagccca atctaagaat cctgctaaga atgaactcta tctagttgag ggggactctg
     2401 ccggtggttc tgccaaacaa ggtcgtgacc gcaagttcca ggctattcta cctcttcgtg
     2461 gtaaggttat caatacagcc aaggccaaga tggcggatat cctcaaaaat gaagagatca
     2521 ataccatgat ttataccatt ggtgcgggtg ttggagcaga cttctctatt gaagatgcca
     2581 actatgataa gatcattatc atgaccgatg cggataccga cggtgcccat atccagacct
     2641 tgctcttgac atttttctac cgttacatgc gtccgctagt cgaggcaggt catgtctata
     2701 ttgccctccc acctctttac aagatgtcca aaggtaaagg aaagaaagaa gaagtggcct
     2761 acgcttggac ggacggagaa ctagaagaac tccgtaaaca gttcggtaaa ggcgctaccc
     2821 tccaacgata caaaggactt ggtgagatga atgcggacca gctctgggaa acaaccatga
     2881 acccagaaac acgtaccctc atccgtgtca caattgaaga tttagcgcgc gccgaacgcc
     2941 gcgtcaatgt tctcatggga gataaggtag aaccacgccg taaatggatt gaagataatg
     3001 tcaagtttac gctagaagaa gcgacagtgt tttaatgaaa gaaaataata tgttagtaaa
     3061 ctggaaatta gagtcggatg tgaatgacta tgtgaaaaag cagtttgaaa atcttggttt
     3121 gaaaaaattg caggattatt aatgaagaat ctgctatgag tgcttatctc aaagaggcgc
     3181 ttaagggagc ggctaagaca caaactaaga ctaattttgg aaaaccagat tttcatattg
     3241 aaaaatacaa acaggatagt ttggttactt atcgtaactg aacacgggag gaattcccgt
     3301 aaaatatctt ttactgcata aaaacctact ctacattctt tgaaaggagt tgaacacgcc
     3361 ctagatactg tgtgaaaaag ataaattctc ttgtgagttt gcttacttca agaattttct
     3421 attttcactt ggtattttat gggctttgta tcttatgtct aacattcaaa acatgtccct
     3481 ggaggacatc atgggagagc gctttggtcg ctactccaag tacattattc aagaccgggc
     3541 tttgccagat attcgtgatg ggttgaagcc ggttcagcgc cgtattcttt attctatgaa
     3601 taaggatagc aatacttttg acaagagcta ccgtaagtcg gccaagtcag tcgggaacat
     3661 catggggaat ttccacccac acggggattc ttctatctat gatgccatgg ttcgtatgtc
     3721 acagaactgg aaaaatcgtg agattctagt tgaaatgcac ggtaataacg gttctatgga
     3781 cggagatcct cctgcggcta tgcgttatac tgaggcacgt ttgtctgaaa ttgcaggtta
     3841 ccttcttcag gatatcgaga aaaatacagt tccttttgca tggaactttg acgatacgga
     3901 gaaagaacca acggtcttgc cagcagcctt tccaaacctc ttggtcaatg gttcgactgg
     3961 gatttcggct ggttatgcca cagacattcc tccccataat ttagctgagg tcatagatgc
     4021 tgcagtttac atgattgacc acccaactgc aaagattgat aaactcatgg aattcttgcc
     4081 tggaccagac ttccctacag gggctattat tcagggtcgt gatgaaatca agaaagctta
     4141 tgagactggg aaagggcgcg tggttgttcg ttccaagact gaaattgaaa agctaaaagg
     4201 tggtaaggaa caaatcgtta ttactgagat tccttatgaa atcaataagg ccaatctagt
     4261 caagaaaatc gatgatgttc gtgttaataa caaggtagct gggattgctg aggttcgtga
     4321 tgagtctgac cgtgatggtc ttcgtatcgc tatcgaactt aagaaagacg ctaatactga
     4381 gcttgttctc aactacttat ttaagtacac cgacctacaa atcaactaca actttaatat
     4441 ggtggcgatt gacaatttca cacctcgtca ggttgggatt gttccaatcc tgtctagcta
     4501 catcgctcac cgtcgagaag tgattttggc gcgttcacgc tttgacaaag aaaaggctga
     4561 gaaacgtctc catatcgtcg aaggtttgat tcgtgtgatt tcgattttgg atgaagtcat
     4621 tgctcttatc cgtgcttctg agaataaggc ggacgccaag gaaaacctca aagttagcta
     4681 tgattttacg gaagaacagg ctgaggctat cgtaactttg caactgtacc gtttgaccaa
     4741 taccgatgtg gttgtcttgc aggaagaaga agcagagctt cgtgagaaga ttgctatgct
     4801 ggcggctatt atcggtgatg aaaggactat gtacaatctc atgaagaaag aacttcgtga
     4861 ggtcaagaag aaatttgcaa ctcctcgttt gagttcttta gaagacactg cgaaagcaat
     4921 tgagattgat acagctagtc ttatcgctga ggaagatacc tacgtcagcg tgaccaaggc
     4981 aggttacatc aagcgtacca gtccacgttc ctttgcggct tccaccttgg aagaaattgg
     5041 caagcgtgat gatgaccgtt tgatttttgt tcaatctgcc aagacaaccc agcacctctt
     5101 gatgttcaca agtcttggaa atgtcatcta cagaccaatc catgagttgg cagatattcg
     5161 ttggaaggac atcggagagc atctgagcca aaccatcaca aactttgaaa cgaatgaaga
     5221 aatcctttat gtggaagtac tggatcagtt tgacgatgcg acaacctact ttgcagtgac
     5281 tcgccttggt caaatcaaac gggtagagcg aaaagaattc actccatggc ggacctatag
     5341 atctaagtct gtcaagtatg ctaagctcaa agacgataca gatcagattg tagcagtggc
     5401 tccgattaaa ctagatgatg ttgtcttggt tagtcaaaat ggttatgccc tgcgtttcaa
     5461 tatcgaagag gttccggttg tcggtgctaa ggcagcaggt gtcaaggcta tgaatttgaa
     5521 agaagatgat gtcctccaat ctggctttat ctgtaatact tcgtccttct acctcttgac
     5581 ccagcgtgga agcttgaaac gtgtttctat tgaggaaatt ctagcaacca gccgtgccaa
     5641 acgaggatta caagtcttgc gtgagttgaa aaacaaaccg catcgtgtct tcttggcagg
     5701 agcagttgca gagcaaggat ttgttggcga tttcttcagt acggaagtgg atgtgaacga
     5761 ccaaactctg cttgtccaat ccaataaagg aacaatctat gaaagccgat tgcaagactt
     5821 gaacttgtca gaacgcacta gcaatggaag cttcatttct gacacgattt cagatgaaga
     5881 agtttttgac gcttatcttc aggaagtagt tactgaagat aaataagtga aatgaagaat
     5941 cagttctaag agctggtttt tattggagaa attttctgaa aattacaaaa tatacttgct
     6001 attttagaaa aatagtgtag aatataagaa ataggttttt agaataagga gtggaatatg
     6061 acagtaacga ttgattggga aaacctcggt ttttcctata tgaaattacc ttatcgctat
     6121 cttgctcatt tcaaaaatgg acaatgggat caaggagagc ttacagagga tgcaactttg
     6181 catatttcag agtcttctcc aagtcttcac tatggacaac aagcatttga aggtttgaaa
     6241 gcttatcgta ctaaggatgg cagtgttcaa ctgttccgtc ctgatgaaaa tgctaaacgt
     6301 ctgcaacgta catgtgaccg tctcttgatg ccacaagttc cgacagacat gtttgtagaa
     6361 gcttgtaaag cagttgtccg tgcgaatgaa gaatacgtac caccatacgg aacaggtgga
     6421 accttatatc ttcgccctct tttgattggt gtcggagata ttatcggggt aaaaccggca
     6481 gaagagtaca ttttcaccat ctttgctatg ccagttggaa attactttaa aggtggtttg
     6541 gtcccaacca acttcttgat tcaggatgag tacgaccgtg cagcaccaaa tggtacaggt
     6601 gcggctaagg ttggtggaaa ctatgctgca agtctcttac caggaaaaat ggccaagtca
     6661 cgtcatttct cagatgttat ctatctggac ccatcaactc atacaaagat tgaagaagtc
     6721 ggatcagcta acttctttgg aattacagct gataatgaat ttgtaacacc attgagtcca
     6781 tctatcttgc catctattac caagtattcc ttgctttatt tggcagaaca tcgcttggga
     6841 ttaactccta ttgagggtga tgttccaatt gataatcttg accgttttgt agaggcaggt
     6901 gcctgtggta cagcagcggt tatttctcca attggaggta ttcaacatgg tgatgatttc
     6961 catgtattct atagtgaaac agaagtaggt cctgtgacgc gtaaattata taatgaattg
     7021 acgggtattc agtttggcga tattgaagcg ccagaaggtt ggattgtaaa agtagattaa
     7081 aataaaccaa aggagatttt ttatgaaata gaaaaagtgg ctcttaacag caggagtggt
     7141 cctgagcacg tcagctattt tagtggcttg tggaaaaact gataaagaag cagatgcacc
     7201 gacaacattt tcttatgtct atgcagtaga tccagcatca ttgggctaca gtatagcgac
     7261 tcgaacatcg aggacagacg ttattggaaa tgttattgat ggtttgatgg aaaatgataa
     7321 atacggcaat gttgctcctt ctcaaaaaga ctatgatttg aacggtacag gatgggctcc
     7381 aagctatcaa gatccagcgt cttacttgaa tattatggat ccaaaatctg gttctgccat
     7441 gaaacacctt ggcattacga aaggaaaaga taaggatgtt gtagctaaac ctggtttgga
     7501 taaatataag aaattgttag aagatgctgt ttctgagacc actgacctag agaagagata
     7561 tgaaaaatat gccaaagctc aagcttggtc gacagatagt tcattattga tgccaacagc
     7621 ttcatctggt ggttttccag ttgtaagtaa cgtagtacca ttctcaaaac catactcaca
     7681 agttggtatt aagggggaac catatatctt taaaggaatg aaattgcaaa aagatattgt
     7741 tacaacaaaa gaatataacg aggtttttaa aaaatggcaa aaagaaaaat tggaatccaa
     7801 tagcaaatac caaaaagaac tagaaaaata cattaaataa ggaatggtat tgatcttgat
     7861 aaaattttta aaatactgtc attttgaata taaaggagtt tgatatggag tggattagat
     7921 taataggaat agcaatcatt gttgtgggtt ttattttaaa atttgataca attgcaacag
     7981 tagtcttagc tggtttggtt acagctttag tttcaggtgt ttctctcgtt gaatttttgg
     8041 agattttggg aaaagaattt agcaatcagc gagtgctcac gatttttatg gttaccttgc
     8101 ctcttgtggg gctgtcagaa acctttggac tcaagcaacg atcaatcgat ttgattcgaa
     8161 agattaaagg tctgacagtt ggaaacttct atacagttta tttctttatt cgagagttag
     8221 ctggtttctt ttcaattcgt ctaggaggac accctcagtt tgtcagacct ttggttcaac
     8281 ctatgggaga agcagctgca gagtctcaat taggtagaaa gttaacagag gttgaagatg
     8341 agacaataaa agcgcgtgcg gctgcgaatg aaaattttgg aaatttcttt gctcaaaata
     8401 cgtttgtagg tgctggggga gtcctcttga tagggggaac attagatcag ttaggctatg
     8461 aaagcaatta tgcagggatt gcttctacat ctattattgt tgctgttata acacttattg
     8521 tagtggggat ttacaattat ttatttgata aaaaattgat atcaaaaaag actaggggag
     8581 gagaacaaaa atgacagagt tggcaaagca actattagag ttgacctata ttgtgattgg
     8641 ttgtcaattt ctccatacag cctattgtag ttataaagat aaaacaaacc cagttcgact
     8701 tgggacatct gcattttgga ctctattgtc tattacgttt ataggtggtt cctatatgcc
     8761 aaatatgagt attggtatta ttgtaatcct attatcgctg ttaacattgt ttaagcaagt
     8821 ccgtatcgga accttgccat ccttagatga aatgaaagcc aatattgaat ctaacaggtt
     8881 gaaaaataaa atttttattc cagttatgct gatggcaata cttgcgttgg tcttagcgca
     8941 aatgattcca gaatttagca agatttcgat tagccttgcc gccttgtttg ctacaatttc
     9001 tgttcttgtg attaccaata gtcaccctaa gagtctgtta tcagaaaata atcgaatgac
     9061 tcagcaagtt tcaacaagtg gtattgttcc tcaattatta ggggctttgg gggctatttt
     9121 tactgtagca ggtgttggtg atgttatctc tcatctgatt agcggtattg ttccttcaga
     9181 tagtcgcttt ataggagttt tggcttatgt tcttggaatg gttctattca caatgattat
     9241 gggaaatgct tttgcagcat tcaccgttat tacagcaggt gttggagttc cctttgtatt
     9301 tgctctggga gctaatccaa ttgtggctgg tgctcttgcc atgacagcag gttattgtgg
     9361 gaccttattg accccaatgg ctgctaattt taacgctcta ccagcagcat tgatggatat
     9421 gaaagatcag aatggcgtta taaaggctca agcaggtgtt gctctagtaa tgattgttat
     9481 tcacatattc ttaatgtact ttctcgcatt ttagtaaagg aaaataaaat gaaaatattg
     9541 gttacaggtt ttaatccttt tggaggtgaa aagattaatc cagctttgga ggctgtaaaa
     9601 ttattaccat ctgagattaa tggggctgaa gttcgctggg tagaaattcc aacggttttt
     9661 tataagtcgt cagaagtttt agaggcagaa atattacgat atcaaccaga tgctgtactt
     9721 tgtattggac aagcaggcgg caggaccggc ttaacacctg aacgagtggc tattaatcaa
     9781 gatgatgctc gcatacctga taacgaaggc aatcaaccaa ttgatacacc gattcgtatt
     9841 gatggagcat cggcctattt tagtagttta cctatcaaag cgatggtaca agctatcaaa
     9901 aaacagggac ttccggcagt tgtatccaat agtgcaggaa cctttgtttg caatcatttg
     9961 atgtaccaag ctctttattt agtggataaa aaattcccta atatgagagc aggtttcatg
    10021 catattccat atatgatgga acaagtagta aataagccga atactgcagg aatgagtcta
    10081 tgtgatattg taagaggcat agaggtagct attgaagcta ttgtagacta caaagataag
    10141 gatttgcagt tagtaggcgg tgagactcac tgataaccta aaaaggatag tcaattatgc
    10201 ttgtttacta actattaact atgctaaatc aattgaggtt gtttacataa aactctatat
    10261 cagagaagcc tgatatagag ttttttcttg ctagttttag gattttcttg taaaatagaa
    10321 aaagtgaaga gaggtatgaa atgagcaaga aagataaaaa aatcgaaatt caagtagcgg
    10381 atgccaaagt taatgttggt aaagacagtt ttgaaggtta tacattgact atcggtaaaa
    10441 aagttatcgg agaaattgcc gaattagacg gacaatttgc cattataaag aatgggaatg
    10501 tcgatagttt ttataaaaaa ttggaaaaag ctgtggaaat tttgattgaa aattataatt
    10561 tagcaaaata agtcttgttt ttttgaaatt ttcatgatat aatagtccat gttgattgta
    10621 ggagagatag cgaagaggct aaacgcggcg gactgtaaat ccgctccttc gggttcgggg
    10681 gttcgaatcc ctctctctcc atttcattaa tggggtatag ccaagcggta aggcaaggga
    10741 ctttgactcc ctcatgcgtt ggttcgaatc cagctacccc agttcttagg taataatcaa
    10801 gataaaaagc aaaatatctt agggtatttt atttttataa ttgaaagacg tgaatgatat
    10861 gaacatgtcc ttgcgggtgc ttaggaaaaa aattataagt atgtcaagtt taagaaaaac
    10921 ttgattgttg gaggattttt tagatgaacg aatttgaaga tttgctaaat agcgttagtc
    10981 aagttgagac tggtgatgtt gttagtgctg aagtattgac agttgatgcg actcaagcta
    11041 acgttgcaat ctctggaact ggtgttgaag gtgtcttgac tcttcgcgaa ttgacaaacg
    11101 atcgtgatgc agatatcaat gactttgtta aagtaggaga agtattggat gttcttgtac
    11161 ttcgtcaagt agttggtaaa gatactgata cagttacata ccttgtatct aaaaaacgcc
    11221 ttgaagctcg caaagcatgg gacaaacttg ttggtcgcga agaagaagtt gttactgtta
    11281 aaggaacgcg tgccgttaaa ggtggacttt cagtagaatt tgaaggtgtt cgtggattta
    11341 tcccagcttc aatgttggat actcgtttcg tacgtaacgc tgagcgtttt gtaggtcaag
    11401 aatttgatac taaaatcaaa gaagttaacg ctaaagaaaa ccgcttcatc ctttcacgtc
    11461 gtgaagttgt tgaagcagct actgcagcag ctcgcgctga agtattcggt aaattggctg
    11521 ttggtgatgt tgtaactggt aaagttgctc gtatcacaag cttcggcgct ttcatcgacc
    11581 ttggtggtgt tgacggattg gttcacttga ctgaattgtc acatgaacgt aacgtatcac
    11641 caaaatcagt tgtaactgtt ggtgaagaaa ttgaagtgaa aatccttgat cttaacgaag
    11701 aagaaggacg tgtatcactt tcacttaaag caacagtacc aggaccatgg gatggcgttg
    11761 agcaaaaatt ggctaaaggt gatgtagtag aaggaacagt taaacgtttg actgacttcg
    11821 gtgcatttgt tgaagtattg ccaggtatcg atggacttgt tcacgtatca caaatttcac
    11881 acaagcggat tgaaaatcca aaagaagctc ttaaagttgg tcaagaagtt caagttaaag
    11941 ttcttgaagt taacgcagat gcagaacgcg tgtcactttc tattaaagct cttgaagaac
    12001 gtccagccca agaagaagga caaaaagaag aaaaacgtgc tgctcgtcca cgtcgtccaa
    12061 gacgtcaaga aaagcgtgat ttcgaacttc cagaaacaca aacaggattt tcaatggctg
    12121 atttgtttgg tgatatcgaa ctttaatcaa attgaaaatt cacaaaatcc tttgtttact
    12181 aaacaaggga tttttctttt gtctctgctc cttttttgat ataatagttc tatgttaaaa
    12241 tcagaaaaac aatcacgtta tcaaatgtta aatgaagaat tgtccttcct attggaaggc
    12301 gaaaccaatg ttttggctaa tctttccaac gccagtgctc ttataaaatc acgttttcct
    12361 aataccgtat ttgcaggctt ttatttgttc gatggaaagg aattggtttt aggtcccttc
    12421 caaggaggtg tttcctgcat ccgtattgca ctaggcaagg gtgtttgtgg tgaggcagct
    12481 cactttcagg aaactgttat tgttggagat gtgacgacct atctcaacta tatttcttgt
    12541 gatagtctag ctaaaagtga aattgtggtg ccgatgatga agaatggtca gttacttgga
    12601 gttctggatc tggattcttc agagattgag gattacgatg ctatggatcg agattatttg
    12661 gaacaatttg tcgctatttt gcttgaaaag acaacatggg actttacgat gtttgaggaa
    12721 aaatcttaat gtatcaagca ctttatcgaa aatatagaag tcaaaacttc tcccagttag
    12781 ttggtcaaga agttgtggct aagactctta aacaagcggt ggagcaagag aaaataagtc
    12841 acgcttatct tttttctggt cctcgtggaa cgggaaaaac cagtgttgct aaaatctttg
    12901 ccaaggctat gaactgtccc aatcaagtgg gtggcgaacc ttgcaataac tgctatattt
    12961 gtcaagcagt gacggacggt agtttagaag atgtcattga aatggatgca gcttctaata
    13021 atggggtaga tgaaattcgc gaaattcgtg ataaatctac ctatgcgcct agccttgctc
    13081 gttataaggt ttatatcata gatgaggttc acatgctgtc tacaggggct tttaatgccc
    13141 tcctaaagac gctggaagaa ccaacacaga atgtagtctt tattttggcc actactgaat
    13201 tgcacaagat tcctgctact attctatccc gtgtgcaacg ttttgagttt aaatcaatta
    13261 agacacagga tattaaggaa catattcact atatcttaga aaaagaaaat atcagttctg
    13321 aaccagaggc tgtggaaatc attgccagac gggcggaagg tggaatgcgg gacgccttgt
    13381 ctattttgga tcaagccctg agtttgacac agggaaatga gctgacgact gctatctctg
    13441 aagaaattac tggcaccatt agcctatcag ccttggatga ttatgtggcg gccttgtctc
    13501 aacaggatgt tcccaaagct ttgtcttgct tgaatcttct ttttgacaat ggtaagagca
    13561 tgactcgttt tgtgaccgat cttttgcact atttaagaga cttgttaatt gttcaaacag
    13621 ggggagaaaa tactcatcat agttcagtct ttgtagaaaa tttggcactt cctcaaaaaa
    13681 atctgtttga aatgattcgc ttagcaacag tgaatttagc agatattaag tctagtttgc
    13741 agcccaagat ttatgctgaa atgatgaccg tccgtttggc ggaaatcaag cccgaaccag
    13801 ctctatcagg agcggttgaa aatgaaattg ctacgctgag acaggaagtt gcccgtctca
    13861 aacaagagct ttctaatgca ggtgcggttc ctaaacaagt tgcaccagct cctagtcgac
    13921 cagctacggg caaaacagtc tatcgtgtcg atcgcaataa agtgcaatct atcttacaag
    13981 aggccgtcga aaatcctgat ttagcacgtc aaaatctaat tcgtttgcag aatgcctggg
    14041 gagaggtaat tgaaagtcta ggtgggccgg acaaggctct gctagttggt tctcaaccgg
    14101 ttgctgccaa tgaacaccat gctattcttg cttttgagtc taacttcaat gctggtcaaa
    14161 ctatgaaacg agacaatctc aataccatgt ttggtaatat cctcagtcag gcggcaggtt
    14221 tttcacctga gattttagct atttccatgg aggaatggaa agaagttcgc gcagcctttt
    14281 cagccaaagc caaatcttct caaactgaaa aagaagtaga agaaagcctg attccagaag
    14341 gatttgaatt tttggctgat aaagtgaagg tagaggaaga ctaaagaaag atttcatgat
    14401 acaataagtt tatgaataaa caacaattta ttattatggc gctatttaca gctgctgaga
    14461 cctatttttt caatgaagcc tggatgactg gccgctatat tatggcagcc ttttgggcaa
    14521 ttttactctt tagaaatttc cgagtcagtt atgtgatggg taaaattgtt gatgtcatcg
    14581 atcagcattt taataggaaa gactagccct cagcttccag acaaaatcaa agccttttag
    14641 gctttttttt gttatactag aaaagtatat ttatagaatt tttgctctat ttctggggaa
    14701 atcagacgtt tttctagtaa gtactgtaaa agttttgaaa aagaaaggaa ctatcatgtc
    14761 agtaatagag atcaaagatc ttcacgttga gattgaagga aaagaaattt taaaaggggt
    14821 taacctgacc ctgaaaacag gagaaattgc cgctatcatg ggaccaaatg gtacaggtaa
    14881 atcgactctt tctgccgcta tcatgggaaa tccaaactat gaagtaacta aaggtgaagt
    14941 tttgtttgat ggcgtaaaca tccttgagtt ggaagtggat gagcgtgcgc gtatgggact
    15001 tttccttgct atgcaatacc catcagaaat ccctggaatt accaatgctg agtttcttcg
    15061 tgccgctatg aatgcgggta aagaagatga tgagaagatt tcagttcgtg agtttattac
    15121 taagctagat gaaaaaatgg aattgctcaa catgaaagaa gaaatggcag agcgttacct
    15181 caacgaaggc ttctctggtg gtgagaaaaa acgcaatgaa attcttcaac ttttgatgtt
    15241 ggagccaaca tttgctcttt tggacgagat tgactcaggt cttgatattg acgctcttaa
    15301 agttgtgtct aaaggtgtca atgccatgcg tggtgaaggt tttggtgcta tgatcatcac
    15361 tcactaccaa cgtcttttga actatatcac acctgatgtg gtacacgtga tgatggaagg
    15421 tcgtgttgtc ctttctggtg gtccagaatt ggctgcgcgt ttggaacgtg aaggatacgc
    15481 aaaattagct gaagaacttg gctacgacta caaggaagaa ttgtaatttc ctcgtatctt
    15541 ttaggagaag taaatgacta gagaaaatat taaacttttt tcagaaatgc acgctgaacc
    15601 aagctggttg gctgatctcc gtcaaaaagc ttttgacaag attgagactt tggaattacc
    15661 agttattgag cgtgtcaaat tccaccgttg gaatctgggt gatggaacga ttacagaaaa
    15721 tgagccatca gcaaatgttc cagatttcac agctttagat catcacttga agttggtgca
    15781 agtaggaact caaactgttt tcgaacaaac tccagttgag ttagctgaac agggtgttgt
    15841 cttcacagac tttcactcag ctttagaaga aattccagag ctgatcgaag aattcttcat
    15901 gtcatctgtt aagtatgatg atgacaagtt ggcggcttac cacacagctt actttaacag
    15961 tggtgctgta ctctatattc cagataacgt agaaatcaca gagccaattg aaggaatttt
    16021 ctaccaagat agcgatagca atgtgccgtt taacaagcat attatgatta tcgctggtaa
    16081 aaattctaag attagttatc tggagcgttt agagtcacgc ggtgaaggaa gtgacaaagc
    16141 aactgccaat atcacagtgg aagtgattgc acattctggt gcgcaagtca agtttgctgc
    16201 tatcgaccgt ctaggtgaaa acgtcactgc ctacattagc cgtcgtggta aattaggcaa
    16261 cgatgcaagt attgactggg ctatcggtgt catgaacgaa ggaaatgtcg ttgctgattt
    16321 tgatagtgac ttgattggta atggtagcta tgctgacctc aaggttgtag ctctttcaag
    16381 tggtcgtcag gtacaaggga ttgatactcg tgtaactaac tatggctgca actctatcgg
    16441 aaatatccta caacatgggg ttatccttga aaaagcaact ttgactttca atggtatcgg
    16501 ccacatcatc aagggtgcta agggagcaga tgcccaacaa gagagccgtg ttctcatgct
    16561 ttcagaccaa gcgcgttcag atgctaaccc aattcttttg attgatgaaa atgatgtcac
    16621 tgcaggacac gcagcgtcta tcggacaagt tgatccagaa gacatgtatt acctcatgag
    16681 tcgtggattg gataaggcaa ctgcagagcg tttggttgtt cgtggtttcc ttggatctgt
    16741 tatcgtggag attccagtca aggaagttcg tgatgaaatg attgcaacta tcgaagagaa
    16801 attgtcaaaa cgctaagggg aagcctatgt tagatgtaga agcgattcgc aaggattttc
    16861 caattttaga tcagattgtc aatgatgaac ctctggtcta tctggacaat gctgcgacga
    16921 cacaaaaacc actagtagtt ctgaaagcta ttaacagtta ctatgagcag gacaatgcca
    16981 atgttcaccg tggtgtccat accttagcgg aacgagcgac agcttcttat gaagctgctc
    17041 gtgaaaccat tcgtaagttt attaatgcag gctctacaaa ggaagttctc tttaccagag
    17101 gaacgacaac cagccttaac tgggtggcac gctttgctga ggaaattctc actgagggag
    17161 accaggtctt gatttcagta atggaacacc attctaatat cattccatgg caggaagctt
    17221 gtcgaaagac tggagcagag cttgtctatg tctatcttaa agacggtgcc ttggatatgg
    17281 aggatttgcg agctaaattg actgataagg ttaaatttgt ttccctagct catgcctcca
    17341 atgttcttgg tgtggtcaat ccgatcaagg aaatcactca attagcccac caagttgggg
    17401 caatcatggt agtggatggt gctcaatcta cacctcatat gaagattgat gtccaggact
    17461 tggatctgga ctttttcgcc ttttcgggtc acaagatggc tggtccgact ggtatcggtg
    17521 tcctttacgg caaagaaaag tatcttgagc aaatgtctcc agtagaattt ggcggcgaga
    17581 tgattgattt tgtctacgag caatccgcta gttggaagga attgccttgg aaatttgagg
    17641 ctggaacgcc aaatatggca ggagctattg gacttgcgac tgcagttgat tatctggaaa
    17701 agattggtat ggatgccgtt gaagctcatg aacaggaatt gattgcgtac gtctatccaa
    17761 aactgcaggc aattgaggga ttgaccattt acggttctca ggatttggct caacgttcgg
    17821 gtgttattgc ctttaaccta ggtgatctcc atcctcacga tcttgcgacg gctctggatt
    17881 atgaaggagt ggctgttcgt gctggtcacc attgtgcgca acccttgctt cagtatttgg
    17941 aagtctcagc aacagctcgt gcaagttttt atatctataa taccaaggca gattgcgaca
    18001 aactagtcga tgccctacaa aagacaaagg agtttttcaa tggcactttc taaactagat
    18061 agcctttata tggcagtggt agcagaccat tcgaaaaatc cacatcacca agggaagtta
    18121 gaagacgctg agcaaatcag tctcaacaat ccgacttgtg gagatgtcat caatctctct
    18181 gtcaagtttg atgcagagga ccgtttggaa gatattgctt ttctaaattc aggatgcacg
    18241 atttcaactg cttctgctag tatgatgaca gatgccgttt taggaaaaac caaacaagaa
    18301 attttagaac tggcgactat tttttctgaa atggttcaag ggcaaaaaga tgagcgtcaa
    18361 gaccaacttg gagacgcggc attcttgtca ggtgttgcca aattccctca aagaatcaag
    18421 tgtgcaaccc tagcttggaa tgcccttaag aaaacaattg aaaatcaaga aaaacagtaa
    18481 gacaagtttc ttttgtctta tgaattatta gaaatgaaga aagaaaggat attatggctg
    18541 aagaaagagt agaaccaaaa ccaattgacc ttggtgaata taaatttggt ttccatgacg
    18601 atgtagagcc tgtcttatcg acaggaaaag gactcaacga aggtgttatt cgtgaattat
    18661 ctgctgctaa gggtgagcct gagtggatgt tggagttccg tttgaagtct tatgaaacct
    18721 tcaaaaaaat gcccatgcaa acttggggag cagacttgtc agagattgac tttgatgact
    18781 taatctacta ccaaaaacca tctgacaaac cagcccgttc ttgggatgat gtacctgaaa
    18841 agattaaaga aacctttgaa cgtatcggga ttccagaagc tgaacgtgct tatttagcag
    18901 gggcttctgc ccagtacgag tcagaagtgg tttaccacaa catgaaggaa gagttccaaa
    18961 aattaggtat tatctttaca gatacagatt ccgcactcaa ggaataccca gacttattta
    19021 atcaatactt tgcgaagttg gtaccgccga cagataacaa gttggcagcc ctcaactcag
    19081 cagtatggtc gggtggaact tttatctacg tgccaaaagg tgtcaaggta gatattccac
    19141 ttcaaactta tttccgtatc aataacgaaa atataggtca gttcgaacgt accttgatta
    19201 tcgttgatga gggagcaagc gtccactacg tagaaggatg tacagcacca acatattcaa
    19261 gcaatagctt acacgctgcc attgtagaaa tttttgcttt ggacggagct tatatgcgtt
    19321 atacaactat ccaaaactgg tctgataacg tctataactt ggtaacaaag cgtgctaagg
    19381 ctcaaaagga tgccactgtt gagtggattg atggaaactt gggtgccaaa acgactatga
    19441 aatatccatc tgtttacctt gatggagaag gagcgcgtgg taccatgctc tctatcgcct
    19501 ttgctaatgc agggcaacac caagacacgg gtgctaagat gattcacaat gctccacata
    19561 ccagctcgtc tattgtgtct aaatccatcg ctaaaggtgg aggaaaggtt gactaccgtg
    19621 gacaagtcac ctttaacaag aactctaaga aatctgtttc ccacattgaa tgtgatacca
    19681 ttatcatgga tgacttgtca gcatcagata ctattccatt taatgaaatt cacaactcgc
    19741 aagtggcttt ggaacacgaa gccaaagtat ctaagatttc agaagagcaa ttgtattatc
    19801 tcatgagccg tggattgtca gaatctgagg caactgaaat gattgtcatg ggatttgtag
    19861 aaccctttac aaaagaactt ccaatggaat acgcagttga gctgaaccgc ttgattagct
    19921 atgaaatgga gggatcagtt ggataaaatt tgattttata ctcttcgaaa atctcttcaa
    19981 accacgtcag catcgcctta tcgtatgtat ggttactgac ttcgtcagtt tcatctacaa
    20041 cctcaaaaca gtgttttgag caacctgcgg ctagcttcct agtttgttct ttgattttga
    20101 agatgaactt gtatcaaaaa atcgcggttt aaaatcgcga ttttttataa tttctcgtta
    20161 acaaagcgga caaactgatt ccaccaaact tttaagaaga aggctttttc aattttcttg
    20221 tctgctacca tttcgaaact agggcgctct gtggtgatgt aaccttgacc aatcaagtcc
    20281 ttgtcttcat aagtcaaatg gccaaccact gttccagctt caagtggtgc tgggattgct
    20341 ttggaatcag gtgtgaattg aacagattga gaagattgat tcccaacacg ttcgattaga
    20401 tagatatcct ctggagccac tgcagttact gtatcttctt ttccatcttg tacaggggct
    20461 ttgctatctt gataggcatc gccttgttga acgattttgc gaagtgtaaa tgtagaagaa
    20521 atataatcca ttagggaaga tgtagctgta aatcgagcgt aaggattatt gtcttgatga
    20581 tctgcattta aaacaactgt gatgactctc atgccttttt cgacagtagt accaacaaaa
    20641 gactctccag ccttatctgt tgttcctgtt tttagcccat caaaaccacc acggtaagca
    20701 ggcatacctt ctaacatgta gttggttgaa gtgattgtca tcccagcaaa agtagaagaa
    20761 ggttttttgg tgatttctaa gacttgtggg tattttttga tgaggttgcg agcaacgata
    20821 gcgacatcat aagcactaag cttattttcc tcatcttttt tagaacctgg gtaaatgtta
    20881 tcccctagag tttcattgtt aagacctgtc gtattgacaa cagtggcatc ctgaattccc
    20941 cattccaaga gttttgcccg catcatatcg acgaaatctt tttctgagcc agcaattttc
    21001 tcagctaggg caatagcggc gctgttggca ctagatacca gagttgcttc aagcaactct
    21061 tcgacagtat aattacgggc ctccatagga atattactgg cttcagaatt tgtcgtcaat
    21121 tgataaggat aatcagaaat atctacagga gtggagaggg taatacttcc gttttccaaa
    21181 gcttcataga ctagataaac agtaatcaat tttgttatgg aagcaatttc gacaggttgc
    21241 gttgcatcct tctcatagag aattttacca gtatttgcct caacagcaat cgcatgttta
    21301 gcggcaatgg taaaatcttg agcaacagca gtagaaacac cccctaaaag agagacagtt
    21361 aacaaagtta aaaatatttt tttcatagta gtcttattct atcataaaga aaaaaaatat
    21421 tcttgcttta ataattcatc tgttaagctt tttgaaaata tggtaaaata aagtgaggga
    21481 ggtaactcat gtttcgtaga aataaattat ttttttggac cacagaaatt ttactcttaa
    21541 ccatcatctt ttacctatgg agacagatgg gatctttgat taaccctttt gttagcgtgc
    21601 ttaatacaat tatgattcca tttttattag ggggctttct ttattatttg acaaacccta
    21661 ttgttacttt cttaaataaa gtctgtaaac tcaatcgttt gcttggtatt ttaattacct
    21721 tgtgtacttt ggtctgggga atggtcatag gtgttgtcta tctcttacct attttgatta
    21781 atcagttatc tagtttgatt atatctagtc aaactattta tagtcgagta caagacttaa
    21841 tcatagactt atctaattat cctgcgctcc agaatttgga tgtagaagct acaattcagc
    21901 agttaaactt atcctatgtt gatattcttc aaaatatcct aaatagcgta tcaaatagtg
    21961 tggggagcgt cttgtcagct cttatcagta ctgttttgat tttgattatg actccagttt
    22021 ttttggttta tttcttatta gatggacata aattcttgcc catgcttgaa agaacgattc
    22081 taaagaggga tcgcttgcat attgcaggct tattaaagaa tttaaatgcg acgattgctc
    22141 gctatattag tggagtttcg attgacgcaa tcattatagg ttgtttggct tatattggct
    22201 atagtattat tggtttaaaa tatgctttag tttttgccat tttttctggt gtagccaatt
    22261 taattcctta tgtggggcca agtattggtt tgattcctat gatcatcgca aatatattca
    22321 ctgatcccca tagactgctg attgcagtga tttatatgct tgttgttcag caggtagatg
    22381 gcaatatctt atatcctcga attgtaggaa gtgttatgaa ggttcatcca atcacgattt
    22441 tagttttact tttgttgtca agcaatatct atggtgtagt tggaatgatt gtcgcagtgc
    22501 caacctattc tatcttgaaa gaaatttcta agttcttatc ccgtttgtat gaaaatcata
    22561 aaataatgaa agaacgagaa agagaattag ctaagtaaaa gtcaggagaa ccctgatttt
    22621 tctttactgg aagtggcctt tagattagaa gactgaaaat aagttaaagt cttaaactaa
    22681 ttttcacagc taagaatagt agaagttaat ctgataaaaa tcgaaaaaac cagtggaatt
    22741 ccgtgtcagg gtaagttcca ctggttttca tagtctatta aagttcgaat gaaacctatt
    22801 tatagtatat tgaaactaga atagtacacc tctaattcta aaacattgtt agaaatcgat
    22861 ttgactgtcc tgatctattc gtcctattct tatttcattt tactatattt tggtgcaata
    22921 agtgaaaagt agtccgaata atataaggat tgattttata gtttttaaac tcaaatgaat
    22981 taaaataaag agagtacgaa aattctcatc tgaaagtatt ttagaataat tctcttcgtg
    23041 aatttcttca aaacagatag cttcatctta ggtatgtgtt aattggattt cgtaagctct
    23101 atttacaatc gtaaagttat gcactgaaaa agttgtagtt agtcttccag tttgctaaat
    23161 ttctcgaata gtatcttttt taggtatgtg atttcttttt gcatttttga gttagataag
    23221 gtataatggt tttattgtct tttggggtcg ttacggattc gacaggcatt atgaggcata
    23281 ttttgcgact cgtgtggcga cgtaaacgct cagttaaata taactgcaaa aaataacact
    23341 tcttacgctc tagctgccta aaaaccagca ggcgtgaccc gatttggatt gctcgtgttc
    23401 aatgacaggt cttattatta gcgagatacg attaagcctt gtctagcggc ttgataagag
    23461 attgatagac tcgcagtttc tagacttgag ttatgtgtcg aggggctgtt aaaataatac
    23521 ataacctatg gttgtagaca aatatgttgg caggtgtttg gacgtgggtt cgactcccac
    23581 cggctccatt attcctttgc attcttttgc attccttggt aaaacgttgt taaatcaacg
    23641 ttttttattt ttatctttgg tattcctttg cattcttttg ctaaaaaggg agtcacaaac
    23701 agaccctatt ttaaaaaaag gatagaaaaa aggatacaac atttgtcgca tcctaaaaat
    23761 aatctttttt cgacggaaga catgggattc gaacccacgc acgctattac acgcctaccg
    23821 cgtttccaac acggcctctt aagcctcttg agtaatcttc caatacttac tcaaatagtc
    23881 taccataaag gctcttatct tgcaataaaa attctagaaa taagaaaaat gatagatttt
    23941 gaaagaaaat gataaaaaat gcttgacttc aaaagaaagt atgatagaat gaatagtgta
    24001 aacgataaca ggaggtgatt cggtgttaaa aacagagcgg aagcaactga ttttagagga
    24061 gttaaatcaa catcatgtag tttctctaga aaaattagtt agtttgctag aaacgtcaga
    24121 atcaacggtt cgaagagact tggatgagtt ggaagcggaa aacaagcttc gtcgtgtgca
    24181 tggtggagca gaactccccc actccttaca ggaagaagaa accattcaag aaaaatcggt
    24241 caaaaacctt caagaaaaga agttgctggc tcagaaagca gcctctctca ttaaagaaaa
    24301 agatgtcatc tttatcgatg ctggaacaac aactgctttt ttgattcatg aattggtcaa
    24361 taagaatgtt acagttgtga ccaactccat tcaccatgcc gctcagttgg ttgaaaagca
    24421 gattccaact gtcatggttg gaggaaacgt caagacggcg acagatgcta gtatcggggg
    24481 cgttgctctt aaccagatta accaattgca ctttgaccgt gcctttatcg gaatgaatgg
    24541 tgttgacgat ggctattata cgactcctga tatggaggag ggagctgtga aaagagctat
    24601 tttggagaat gccaagcaga cctacgtctt ggtggattcg tcaaaaattg gacaaacttg
    24661 ctttgccaag gtagcaccac tcaaacgcgc tatcgttatc actagtcaag ggcatgagct
    24721 cttgcaggtt attaaggaga aaacggaggt aatagaagta tgatttatac agtcacactc
    24781 aatccatcca ttgactatat cgttcgtttg gaccaagtca aagttggtag tgtaaatcgt
    24841 atggacagtg atgataagtt tgctggtggg aaaggaatca atgtcagccg tgtcttgaaa
    24901 cgtttgaata taccaaatac agcgacggga tttatcggtg gctttactgg taaatttatc
    24961 acagatactt tagcagagga agaaatcgag acacgttttg tccaggtggc agaagatact
    25021 cgtatcaatg ttaaaatcaa agcagaccaa gaaacagaaa tcaacggaac gggtccaact
    25081 gttgaaccgg ttcagctaga agaattgaaa gctattttat ctagtctgac agcagaagat
    25141 acagttgtct ttgcaggttc aagtgctaaa aatctaggca atgttatcta taaggatttg
    25201 atttccttga cgcgccagac tggtgcgcaa gtggtctgtg actttgaagg acagacctta
    25261 attgatagtt tggactacca gcctcttctt gtaaaaccaa acaatcatga acttggagcg
    25321 atttttgggg ttaaactcga aagtttagat gaaattgaga aatacgctcg tgagttactg
    25381 gctaagggtg ctcaaaatgt tattatctct atggctggtg atggcgccct tcttgtcaca
    25441 tctgagggag cttacttcgc taaaccaatc aaaggaacag tcaaaaattc agttggagct
    25501 ggtgattcta tggttgctgg attcacaggt gaatttgtca aatcaaaaga cgcagtagaa
    25561 gccttcaaat ggggagtggc ttgcggaacg gcaactacct tctcagatga cttggcaacg
    25621 gcggaattta ttaaagaaac atatggaaaa gttgaggtag aaaaaagatg aaaattcaag
    25681 acctattgag aaaagatgtc atgttgctag atttgcaggc aactgaaaaa acagctgtca
    25741 tcgacgagat gattaaaaat ttgacagagc acggttatgt aacagatttt gaaacattta
    25801 aagaaggaat tttggcgcgt gaagctttga cttctactgg tttgggtgat ggaatcgcaa
    25861 tgcctcacag caaaaacgct gctgtcaaag aagcgacagt tctatttgct aagtcaaata
    25921 agggtgttga ctacgagagc ttggatggac aagcaactga cctcttcttc atgattgcag
    25981 ctccagaagg tgccaatgat actcacttgg cagccttggc agaattgtct caatacttga
    26041 tgaaagacgg ttttgcagac aaacttcgtc aagcaacatc tgcagaccaa gttatcgaac
    26101 tttttgacca agcttcagaa aaaactgagg aacttgttca agcacctgct aatgactctg
    26161 gtgactttat cgtagctgtt acagcttgta caacaggtat tgcccacact tacatggccc
    26221 aagaagccct tcaaaaagta gctgctgaaa tgggggttgg tatcaaggtc gaaaccaacg
    26281 gtgctagcgg tgttggaaat caactaactg cagaagatat ccgtaaggct aaagctatta
    26341 tcattgcagc agacaaggcc gttgaaatgg atcgatttga tggaaaacca ttgatcaatc
    26401 gtccagttgc tgacggtatc cgtaagacag aagagctaat taacttggct ctttcaggag
    26461 atactgaagt ctaccgtgcc gctaatggtg ccaaagctgc aacagcctct aacgaaaaac
    26521 aaagccttgg tggtgccttg tacaaacact tgatgagtgg tgtatctcaa atgttaccat
    26581 tcgttatcgg tggtggtatc atgattgccc ttgccttctt gattgacggt gctttgggtg
    26641 ttccaaatga aaaccttggc aatcttggtt cttaccatga gttagcttct atgttcatga
    26701 aaattggtgg agctgccttt ggtttgatgc ttccagtctt tgcgggttat gttgcctact
    26761 ctattgctga aaaaccgggt ttggtagcag gtttcgtggc tggtgctatt gccaaagaag
    26821 gttttgcctt tggtaaaatt ccttatgccg caggtggtga agcaacttca actcttgcag
    26881 gtgtctcatc tggtttccta ggtgcccttg ttggtggatt tatcgcaggt gccttggttc
    26941 ttgccatcaa gaaatacgtt aaagttcctc gttcactcga aggtgctaaa tcaatccttc
    27001 tattgccact tcttggaaca atcttgacag gatttgttat gctagctgtg aatatcccaa
    27061 tggctgcaat caacactgct atgaatgact tcctaggcgg tcttggagga ggttcagctg
    27121 tccttcttgg tatcgtcctt ggtggaatga tggctgttga catgggtgga ccagttaata
    27181 aagcagctta tgtctttggt acaggtacgc ttgcagcaac tgtttcttca ggtggttctg
    27241 tagccatggc agcagttatg gctggaggaa tggtgccacc acttgcaatc tttgtcgcaa
    27301 ctcttctttt caaagataaa tttactaagg aagaacgtaa ctctggtttg acaaacatca
    27361 tcatgggctt gtcatttatc actgagggag cgattccatt tggtgccgct gacccagctc
    27421 gtgcgattcc aagcttcatc cttggttcag cagtagcagg tggactcgtt ggtcttactg
    27481 gtatcaaact catggcgcca cacggaggaa tcttcgttat cgcccttact tcaaatgctc
    27541 tcctttacct cgtttctgtc ttggtaggag caatcgtaag tggtgtggtt tatggttacc
    27601 tacgcaaacc acaagcataa aaaatagaaa aatgaaaaga ttggaccgtt tggtgcagtc
    27661 tttttctctt cccgaaatgc ctgtgaaata tggtataata gaagaatggc aaacaagaat
    27721 acaagtacaa caagacggag accgtctaaa gcagaactgg aaagaaaaga agcgattcaa
    27781 cgaatgttga tttcgttagg aattgcgatt ttattgattt tcgcagcctt caaattaggg
    27841 gctgcaggta taacccttta taatttaatt cgcttgctag tgggtagcct agcttatctg
    27901 gcgatattcg gcctattaat ctatctcttc tttttcaagt ggatacgaaa acaggaagga
    27961 ctcttatctg gctttttcac catatttgct ggcttactct tgatttttga ggcctacttg
    28021 gtttggaaat atggtttgga caagtccgtt ctaaaaggga ccatggctca ggttgtgaca
    28081 gatctgactg gttttcgaac gactagcttt gctggagggg gcttgatcgg ggtcgctctt
    28141 tatattccaa cagcctttct cttttcaaat atcggaactt actttattgg ttctatcttg
    28201 attttagtgg gttctctcct agtcagccct tggtctgttt acgatattgc tgaatttttc
    28261 agtagaggct ttgccaaatg gtgggaaggg cacgagcgtc gaaaagagga acgctttgtc
    28321 aaacaagaag aaaaagctcg ccaaaaggct gagaaagagg ctagattaga acaagaagag
    28381 actgaaaaag ccttactcga tttgcctcct gttgatatgg aaacgggtga aattctgaca
    28441 gaggaagctg ttcaaaatct tccacctatt ccagaagaaa agtgggtgga accagaaatc
    28501 atcctgcctc aagctgaact taaattccct gaacaggaag atgactcaga tgacgaagat
    28561 gttcaggtcg atttttcagc caaagaagcc cttgaataca aacttccaag cttacaactc
    28621 tttgcaccag ataaaccaaa agatcagtct aaagagaaga aaattgtcag agaaaatatc
    28681 aaaatcttag aagcaacctt tgctagcttt ggtattaagg taacagttga acgggccgaa
    28741 attgggccat cagtgaccaa gtatgaagtc aagccggctg ttggtgtaag ggtcaaccgc
    28801 atttccaatc tatcagatga cctcgctcta gccttggctg ccaaagatgt ccggattgaa
    28861 gcaccaatcc ctgggaaatc cctaatcgga attgaagtgc ccaactccga tattgccact
    28921 gtatctttcc gagaactatg ggaacaatcg caaacgaaag cagaaaattt cttggaaatt
    28981 cctttaggga aggctgttaa tggaaccgca agagcttttg acctttctaa aatgccccac
    29041 ttgctagttg caggttcaac gggttcaggg aagtcagtag cagttaacgg cattattgct
    29101 agcattctca tgaaggcgag accagatcaa gttaaattta tgatggtcga tcccaagatg
    29161 gttgagttat ctgtttacaa tgatattccc cacctcttga ttccagtcgt gaccaatcca
    29221 cgcaaagcca gcaaggctct gcaaaaggtt gtggatgaaa tggaaaaccg ttatgaactc
    29281 tttgccaagg tgggagttcg gaatattgca ggttttaatg ccaaggtaga agagttcaat
    29341 tcccagtctg agtacaagca aattccgcta ccattcattg tcgtgattgt ggatgagttg
    29401 gctgacctca tgatggtggc cagcaaggaa gtggaagatg ctatcatccg tcttgggcag
    29461 aaggcgcgtg ctgcaggtat ccacatgatt cttgcaactc agcgtccatc tgttgatgtc
    29521 atctctggtt tgattaaggc caatgttcca tctcgtgtag catttgcggt ttcatcagga
    29581 acagactccc gtacgatttt ggatgaaaat ggagcagaaa aacttcttgg tcgaggagac
    29641 atgctcttta aaccgattga tgaaaatcat ccagttcgtc tccaaggctc ctttatctcg
    29701 gatgacgatg ttgagcgcat tgtgaacttc atcaagactc aggcagatgc agactacgat
    29761 gagagttttg atccaggtga ggtttctgaa aatgaaggag aattttcgga tggagatgct
    29821 ggtggtgatc cgctttttga agaagctaag tctttggtta tcgaaacaca gaaagccagt
    29881 gcgtctatga ttcagcgtcg tttatcagtt ggatttaacc gtgcgacccg tctcatggaa
    29941 gaactggaga tagcaggtgt catcggtcca gctgaaggta ccaaacctcg aaaagtgtta
    30001 caacaataaa aaaatagctt ctttccaagt ttggagggaa gctatttttc tggctattga
    30061 ttacttttat actcttcgca aatctcttca aaccacgtca gcttcgcctt gccgtactca
    30121 agtactgtct acggctagct tcctagtttg ctctttgatt ttcattgagt attattttct
    30181 gaagttggag tattgggctg tttttcattt tcagtggcag gtttacttgt agcaggtgta
    30241 gtagagtcct gagttgctgt tttctgatct tcttttttct cttccttgac gctagatttt
    30301 ggcgtttctt cttgttgtgt tttttcttga gaagtgttat tttccttatt tggagtagta
    30361 ttttcctgag gggattcttt ttgaatttct ttgacaaagg ttgtcgtctg gcttgtcgta
    30421 ggttcttttt tagtattttt attattatta tccaaggcgt tcatgatcgt gatacccgcg
    30481 gtaatcaaac caaggatact accgatagta gtaatggttt tttgaaaaac agtaaagcct
    30541 tttttgatat gttctgtttt cggttttgaa gttctacgat agttagacat acattctctc
    30601 ctttgattat gatttattat actctgtttc tttaaaaaaa tcttaaaaaa agagaaattg
    30661 ccctttcttg tcgcaggctc cgtttcatga tacaatagaa ggagtgattt tagaaagcgt
    30721 gagaaacatg atttactttg ataattcggc gacgaccagg ccctatccag aagcccttga
    30781 aacctatatg caggtggctt caaaaatttt aggaaatcca tctagtctcc atcgtttggg
    30841 agaccaggca acacgaattt tagatgcttc ccgtcaacag attgcagatt taatcggtaa
    30901 gaaaagtgat gaaatcttct ttacatctgg tgggacagaa ggggataact ggatcatcaa
    30961 gggagtagca tttgaaaaag cccagtttgg caagcacatc atcgtatcag ccattgaaca
    31021 tccagcagtt aaggaatcag ccctctggtt gaaagctcaa ggttttgaag tggattttgc
    31081 tccagttgat aacaaaggat ttgtggatgt tgaggcgcta gaagatttga taagacctga
    31141 tacgaccctc gtttccatca tggctgtgaa caatgaaatc ggctctatcc aacctattga
    31201 ggctatttca gaattcttgg cagacaagcc aactatttcc ttccacgttg atgcggttca
    31261 ggcgcttgcc aagattccga ctgaaaagta tctgacagaa cgagtggatt tcgcgacttt
    31321 ctctggtcac aagttccacg gaatccgtgg tgttggtttt atctatatca agtctggcaa
    31381 gaagattacg cctcttttaa caggtggtgg tcaggaacgt gattaccgtt cgacaactga
    31441 aaatgtggca ggaattgcag cgaccgctaa ggctctccgt ttatctatgg aaaagctaga
    31501 tatctttagg agcaagactg ggcagatgaa ggcagtgatt cgtcaagcac ttctggacta
    31561 tccggatata tttgtctttt cagatgagga agacttcgcc cctcatattc tgacttttgg
    31621 aatcaagggt gttcgtggtg aggtcattgt tcacgccttt gaagactatg atattttcat
    31681 ctcaacgacc tcggcttgtt cgtccaaggc tgggaaacca gccggaaccc tgattgctat
    31741 gggagtggac aaggataagg ctcagtcagc tgtgcgtctt agtctagacc ttgaaaatga
    31801 tatgagtcag gtcgagcagt tcttgaccaa gctaaaatta atttacaatc aaactagaaa
    31861 agtaagatag gagcattcat gcagtattca gaaattatga ttcgctacgg tgagctgtca
    31921 accaagggca aaaatcgtat gcgtttcatc aataaacttc gcaataatat ttcagacgtt
    31981 ttgtctatct atccccaagt taaggtaaca gcagatcgcg accgtgccca cgcttacctc
    32041 aatggagcag attacacagc agtagcagaa tcgctcaaac aagtatttgg aattcaaaac
    32101 ttttcccctg tttataaggt tgaaaaatct gtagaagttc tgaagtctgc tgtccaagag
    32161 attatgcagg acatctacaa ggaaggcatg acctttaaaa tctctagcaa gcgtagcgac
    32221 cacacctttg aattggacag tcgtgaactc aaccaaacgc ttggaggggc tgtattcgaa
    32281 gccattccaa acgtacaagc tcaaatgaaa agtcctgaca tcaatcttca ggtggagata
    32341 cgtgaagaag cagcctatct ttcttatgaa accgttcgag gggcaggtgg attaccagtg
    32401 ggaacttctg gtaagggcat gctcatgttg tcagggggaa tcgattcccc tgtagctggc
    32461 tatctagcgc ttaaacgagg ggtagatata gaggcagttc actttgccag cccaccttac
    32521 acgagtcctg gtgctcttaa aaaagcccaa gatttgaccc gtaaattaac caaatttggg
    32581 ggaaatatcc agtttatcga agttcctttt acagagattc aagaggaaat caaagccaaa
    32641 gcgccagaag cctaccttat gaccttgacg cgtcgtttta tgatgcgtat tactgaccgt
    32701 attcgtgagg tacgaaatgg tttagttatc atcaatgggg aaagtctagg tcaagtagcc
    32761 agccaaaccc ttgaaagtat gaaggctatc aatgctgtta ccaacactcc catcattcgt
    32821 cctgtggtta ccatggacaa gttggaaatc attgacatcg cccaggaaat cgataccttt
    32881 gacatttcaa tccaaccgtt tgaagactgt tgtactattt ttgcaccaga tcgtccaaaa
    32941 acaaatccta aaattaagaa tgcggagcag tacgaagcgc gtatggatgt tgaaggcttg
    33001 gttgagcgag cagtggctgg aatcatgatt actgaaatca cacctcaagc cgaaaaagat
    33061 gaagttgatg acttgattga caatctgctc taattcagaa aatccaaaag aatagcgaaa
    33121 atcagtaaaa aaagttagtt ttttctctaa aaacaggtaa aaaactaact ttttttattt
    33181 ttatgatata atgatataaa attttgaata tagagagttt tctgacaatg aatcaatcct
    33241 acttttatct aaaaatgaaa gaacacaaac tcaaggttcc ttatacaggt aaggagcgcc
    33301 gtgtacgtat tcttcttcct aaagattatg agaaagatac agaccgttcc tatcctgttg
    33361 tatactttca tgacgggcaa aatgttttta atagcaaaga gtctttcatt ggacattcat
    33421 ggaagattat cccagctatc aaacgaaatc cggatatcag tcgcatgatt gtcgttgcta
    33481 ttgacaatga tggtatgggg aggatgaatg agtatgcggc ttggaagttc caagaatctc
    33541 ctatcccaga gcagcagttt ggtggtaagg gtgtggagta tgctgagttt gtcatggagg
    33601 tggtcaagcc ttttatcaag cacaaaacag gttggtttga tggcatgatg acgacaggtt
    33661 gctctatggg agcctatcat gcactcaatt tcttcctcca gcatccagat gtctttacca
    33721 aagtgattgc tctcagtggt gtttacgacg cacgtttctt tgtcggcgat tactacaacg
    33781 atgatgctat ttaccaaaac tcgccagtag attatatttg gaaccaaaac gatggctggt
    33841 ttattgaccg ttaccgtcag gcagagattg tgctgtgtac ggggcttgga gcctgggaac
    33901 aagatggttt accatccttt tacaagctca aagaagcctt tgaccagaaa caaattccag
    33961 cctggtttgc tgaatgggga catgatgtcg cccacgactg ggaatggtgg cgtaaacaaa
    34021 tgccttattt cctcggtaat ctctatttat aaaaggagtt acctatgaat taccttgtta
    34081 tttctcccta ctatccacaa aactttcaac agtttaccat cgaactagct aataaaggca
    34141 tcacagtctt gggaattggt caagagtctt acgagcaatt ggatgagccc ttgcgcaata
    34201 gcttgaccga gtattttcgt gttgataatc ttgagaacat agatgaagtc aaacgtgcag
    34261 ttgcttttct cttttataaa catggtccaa ttgaccgcat cgagtctcac aatgaatact
    34321 ggcttgagct agacgcaaca ctcagagaac aattcaatgt ttttggtgcc aaaccagagg
    34381 atctcaaaaa gacgaaatat aagtctgaaa tgaagaaact tttcaaaaaa gcaggtgttc
    34441 ctgtggtacc tggagctgtt atcaagacgg aagcagatgt tgatcaagca gtgaaagaaa
    34501 tcggtcttcc aatgattgcc aaacctgata atggagtggg agcagccgca acctttaaac
    34561 ttgagacaga agacgatatc aatcacttca agcaagaatg ggaccattca accctttatt
    34621 tctttgaaaa atttgtcact tccagcgaaa tctgtacctt tgacgggctc gtggacaagg
    34681 atggaaagat tgtcttctca acaacctttg actacgccta tacaccgctt gacctcatga
    34741 tttataagat ggacaattct tattatgtgc tcaaggatat ggatcctaaa ctgcgcaagt
    34801 atggggaagc aattgtcaaa gaatttggta tgaaagaacg gtttttccat attgagttct
    34861 tccgtgaggg ggacgattat attaccatcg agtacaataa ccgccctgca ggtggtttta
    34921 ccattgatgt ttataacttt gctcattcct tggaccttta tcgtggctat gcagctattg
    34981 tcgcaggaga ggagttcccg gcgtcagact ttgaaactca gtattgtttg gctacttctc
    35041 gccgtgcaaa tgctcactat gtttattcag aagaggattt gcttgccaaa tatagccagc
    35101 agttcaaggt taaaaaagtc atgccagctg ccttcgcgga acttcaagga gattacctgt
    35161 atatgctgac aactccgagt cgacaagaaa tggatcagat gattgctgat ttcggacaac
    35221 gtcaagaata agaactatcg gattaaggaa attcactccc ttaatccttt tgttttgtct
    35281 ggtaaaaaat aagagcatcc taacaaggta gctatcataa aacttgttcg ataactattt
    35341 gaagcaggat taggtggcta gaaattgaat tttaacattt caatttagtc atagtattgt
    35401 gttttcgtat ccttaaatca gctaaaagga tccatgacga cacctatacg atatagtttt
    35461 caggatacca aaccagtcta tt
//
LOCUS       DAVBNO010000017        34124 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVBNO010000017 DAVBNO010000000
VERSION     DAVBNO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34124
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.017"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(21..833)
                     /locus_tag="VQ846_000939"
     CDS             complement(21..833)
                     /locus_tag="VQ846_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET3075529.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            1023..2441
                     /locus_tag="VQ846_000940"
     CDS             1023..2441
                     /locus_tag="VQ846_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET3075530.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHVMIWILDALFILNYISMAL"
     gene            2494..3201
                     /locus_tag="VQ846_000941"
     CDS             2494..3201
                     /locus_tag="VQ846_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET3075531.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGISIVVLVLFIIGARKTQLASFNFSFFRTKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            3297..4277
                     /gene="folP"
                     /locus_tag="VQ846_000942"
     CDS             3297..4277
                     /gene="folP"
                     /locus_tag="VQ846_000942"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET3075532.1"
                     /translation="MMKSLEGVTDMSSKANHAKTAICGIINVTPDSFSDGGQFFVLEQ
                     ALQQARKLIAEGASMLDIGGESTRPRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISI
                     DTRKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSS
                     LIFPHFGFGQTFTEKELADFETLPIEEMMEAFFERALARAAEAGIAPENILLDPGIGF
                     GLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDT
                     ASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            4279..5601
                     /locus_tag="VQ846_000943"
     CDS             4279..5601
                     /locus_tag="VQ846_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET3075533.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLALLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLDQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            5582..6136
                     /gene="folE"
                     /locus_tag="VQ846_000944"
     CDS             5582..6136
                     /gene="folE"
                     /locus_tag="VQ846_000944"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET3075534.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            6179..6991
                     /gene="folK"
                     /locus_tag="VQ846_000945"
     CDS             6179..6991
                     /gene="folK"
                     /locus_tag="VQ846_000945"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET3075535.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(7036..7353)
                     /locus_tag="VQ846_000946"
     CDS             complement(7036..7353)
                     /locus_tag="VQ846_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET3075536.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            7710..8156
                     /gene="rplM"
                     /locus_tag="VQ846_000947"
     CDS             7710..8156
                     /gene="rplM"
                     /locus_tag="VQ846_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET3075537.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            8175..8567
                     /gene="rpsI"
                     /locus_tag="VQ846_000948"
     CDS             8175..8567
                     /gene="rpsI"
                     /locus_tag="VQ846_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET3075538.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVIGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(8829..9251)
                     /locus_tag="VQ846_000949"
     CDS             complement(8829..9251)
                     /locus_tag="VQ846_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET3075539.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            9398..9574
                     /locus_tag="VQ846_000950"
     CDS             9398..9574
                     /locus_tag="VQ846_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075540.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            9976..12189
                     /locus_tag="VQ846_000951"
     CDS             9976..12189
                     /locus_tag="VQ846_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET3075541.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(12411..12887)
                     /locus_tag="VQ846_000952"
     CDS             complement(12411..12887)
                     /locus_tag="VQ846_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET3075542.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            13110..16313
                     /locus_tag="VQ846_000953"
     CDS             13110..16313
                     /locus_tag="VQ846_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET3075543.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSSWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSHEKLRAALHRMVPIEVKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKGGTTNVIVSKDGKE
                     VKKIPLKILASVKDTYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRPSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVGRATFNQMIKKLESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            16692..17077
                     /gene="tnpA"
                     /locus_tag="VQ846_000954"
                     /pseudo
     CDS             16692..17077
                     /gene="tnpA"
                     /locus_tag="VQ846_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(17456..18085)
                     /locus_tag="VQ846_000955"
     CDS             complement(17456..18085)
                     /locus_tag="VQ846_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET3075544.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(18095..19096)
                     /locus_tag="VQ846_000956"
     CDS             complement(18095..19096)
                     /locus_tag="VQ846_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET3075545.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEELFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(19127..19768)
                     /locus_tag="VQ846_000957"
     CDS             complement(19127..19768)
                     /locus_tag="VQ846_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET3075546.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(19787..20602)
                     /locus_tag="VQ846_000958"
     CDS             complement(19787..20602)
                     /locus_tag="VQ846_000958"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET3075547.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIKAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEIGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            20873..21307
                     /locus_tag="VQ846_000959"
     CDS             20873..21307
                     /locus_tag="VQ846_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET3075548.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            21319..22509
                     /locus_tag="VQ846_000960"
     CDS             21319..22509
                     /locus_tag="VQ846_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET3075549.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREAALKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEYYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            22520..23011
                     /locus_tag="VQ846_000961"
     CDS             22520..23011
                     /locus_tag="VQ846_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET3075550.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            23026..23805
                     /locus_tag="VQ846_000962"
     CDS             23026..23805
                     /locus_tag="VQ846_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET3075551.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            23792..24610
                     /locus_tag="VQ846_000963"
     CDS             23792..24610
                     /locus_tag="VQ846_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET3075552.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            24610..24903
                     /locus_tag="VQ846_000964"
     CDS             24610..24903
                     /locus_tag="VQ846_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET3075553.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            24925..26826
                     /locus_tag="VQ846_000965"
     CDS             24925..26826
                     /locus_tag="VQ846_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET3075554.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALIQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            26886..27887
                     /locus_tag="VQ846_000966"
     CDS             26886..27887
                     /locus_tag="VQ846_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET3075555.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(27997..28332)
                     /locus_tag="VQ846_000967"
     CDS             complement(27997..28332)
                     /locus_tag="VQ846_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075556.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLSNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(28343..28585)
                     /locus_tag="VQ846_000968"
     CDS             complement(28343..28585)
                     /locus_tag="VQ846_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075557.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(28596..29138)
                     /locus_tag="VQ846_000969"
     CDS             complement(28596..29138)
                     /locus_tag="VQ846_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET3075558.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYLGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(29154..29348)
                     /locus_tag="VQ846_000970"
     CDS             complement(29154..29348)
                     /locus_tag="VQ846_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3075559.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            29514..30464
                     /gene="rsmH"
                     /locus_tag="VQ846_000971"
     CDS             29514..30464
                     /gene="rsmH"
                     /locus_tag="VQ846_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET3075560.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            30476..30793
                     /gene="ftsL"
                     /locus_tag="VQ846_000972"
     CDS             30476..30793
                     /gene="ftsL"
                     /locus_tag="VQ846_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET3075561.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            30797..33049
                     /gene="pbpX"
                     /locus_tag="VQ846_000973"
     CDS             30797..33049
                     /gene="pbpX"
                     /locus_tag="VQ846_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET3075562.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETFAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            33051..34031
                     /locus_tag="VQ846_000974"
     CDS             33051..34031
                     /locus_tag="VQ846_000974"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET3075563.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGIIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
BASE COUNT        10306 a         6071 c         7607 g        10140 t
ORIGIN      
        1 tcattgagta tcagtatagg ttataagaat aagtcttgta gggttttggc aaccccatct
       61 tggtcgttgg taagggaaat ttgctcatct gcataaggga gtagctctgg attggcattt
      121 ttcatggcat aacccttccc agcaaaagcg agcatttcgg tatcattgtg ttcatctcca
      181 aaggcaatca aatctttttt gtcacgattc attatcttga gcaagtagtc caaagcaaag
      241 gccttgttga caccttttgg ggtacattca aggatattga gcggacctcc ccaggtattg
      301 atagaaagtt gatgctggta gaaggcgttc atgtcttttg ccaaggcata tttgtcactg
      361 gctctggtct gcaagaggat acagttaggg tccttggtca ccaattcagg ctggaattga
      421 tcttcaggct ggaaagcttc tacaccaaat agtttgggat tggcaatttc ttcattggga
      481 tttgtaatgt agaatttttt acgatattct ccagcgataa aatcggcttg aatgtcctct
      541 gaacgttgaa ccatatctag cagatatttt ttgtctacag tcaaacactt ttcaaaatcc
      601 caaacttggt ctggtaaatg agtaagggat ccgttgaagt taatcatagg agtgtctaag
      661 cccagttcac ggtaaaaatc ttttgacata cggtaagggc gacctgtcgt aataataacc
      721 tgatggcctt tttcagcaac ttttttgatg gtttttttgg taaagtcaga aatttgactg
      781 tctgagttga gcagggttcc atccaggtca actgcaataa tttttttcgt catatggacc
      841 tttctagtgt gttttcagat aagatgtctt ctattataac agaaagcagg cagaaaagca
      901 gattccaaac aaaaaatgaa attggattaa tttcttaaac aaatcaaaca aagacattga
      961 aaaagtgaat gataaatgat ataattctat tattgttcgt aaaaattaaa aggagattga
     1021 tgatggacaa attatttaaa ctaaaagaga acggtacaga cgttcgtaca gaggttctcg
     1081 ctggtttaac aactttcttt gcaatgagct atattctctt tgtaaaccca caaatacttt
     1141 cacaaacagg aatgcctgct cagggcgtct tcctagcgac gattattggt gcagtagcgg
     1201 gtaccctgat gatggctttt tatgctaact taccttatgc ccaagcgcca ggtatgggac
     1261 tcaatgcctt ctttaccttt acagttgtat tcgggcttgg ttattcttgg caagaagccc
     1321 tagctatggt cttcatctgt gggattattt cattgattat taccttgaca aatgttcgta
     1381 aaatgatcat tgaatcgatt cccaatgctc ttcgctcagc tatttcagct ggtatcggtg
     1441 tcttccttgc ctatgtaggg attaagaatg ctggactttt gaaattcacg attgatccag
     1501 gcaactatac tgttgtagga gaaggggctg acaaagctca agcaacgatt gcagcaaact
     1561 cttcagcagt tccaggattg gtcagcttta ataatccagc tgttttagtg gctcttgcag
     1621 gacttgccat tactatcttc tttgtcatca aagggattaa agggggaatt attctctcta
     1681 tcttgacaac aactgttctt gctattgcag ttggtttggt tgatttgtct agtatcgatt
     1741 ttgctaataa ccatgttggt gcagcttttg aagatttgaa gacaatcttt ggtgcagctc
     1801 ttggttcaga aggattggga gctttggttt cagatacagc tcgcttgcct gaaactctga
     1861 tggccattct tgccttctca ttgacagata tttttgacac aattggtacc ttgatcggta
     1921 caggtgaaaa agttggtatc gtagcgacaa atggtgaaaa tcaccaatca gccaaattgg
     1981 ataaggctct ttactctgat ttgattggaa cgacagtcgg tgccattgca ggtacttcaa
     2041 acgtaacgac ttatgttgag tctgctgctg gtatcggtgc tggtggacgt actggtttga
     2101 cagccttggt tgtagctatc tgttttgcga tttcaagctt ctttagccca cttctagcga
     2161 tcgtaccaac agcggctaca gctccaatct tgattatcgt tgggattatg atgcttggta
     2221 gcttgaaaaa tatccattgg gatgatatgt ctgaagcagt tcctgccttc ttcacatcta
     2281 tctttatggg attcagctac tctatcactc aagggattgc agttggtttc ttgacttaca
     2341 ctttgactaa gcttgttaaa ggtcaagtta aagatgttca tgtcatgatt tggattttgg
     2401 atgccttgtt tatccttaac tacatcagca tggccttata atagaatgac ccagggggat
     2461 ttcccccctt ttttaataca aaggagatag gtgatgaaag agaaaaatat gtggaaagaa
     2521 ttgttgaatc gtgcaggctg gattttggtc tttttacttg ccgtcctttt atatcaggtt
     2581 cccctagtgg ttacctctat tttgacttta aaagaagtag ccctgctaca gtcagggctg
     2641 atagttactg gtatttctat tgtggttctg gtgctattta ttattggagc tcgtaaaacg
     2701 caattggcaa gttttaattt ttcttttttt agaactaagg atttggcacg tttgggcttg
     2761 agttatttag tcattatctg ttcaaatata cttggttcta tcttgttgca actgtcaaat
     2821 gagacgacaa cagctaacca gtctcagatt aacgatatgg ttcagaatag ttctctgatt
     2881 tctagcttct tcttgctagc cttgcttgct ccaatttgtg aggaaatctt gtgccgtggg
     2941 attgttccta aaaagatttt ccgaggcaag gagaacttgg gatttgtagt cggtacgatt
     3001 gtgtttgctt tattgcatca accaagtaat ttaccttctt tattgattta tggaggtatg
     3061 tcgacagttc tatcttggac ggcctacaag acccaacgtt tggaaatgtc gatcttgctt
     3121 cacatgattg ttaatgggat tgctttctgt ttgttggctc ttgtggtgat tatgagtcgg
     3181 acattaggaa tttctgttta aaagttttta tgtaggaacc gacctctttc tactgggaaa
     3241 aagatgaatg caatcgtgtc catctttttc tttttatggt aaaatagaga aataatatga
     3301 tgaaaagcct tgagggagtg accgatatgt caagtaaagc taatcatgca aagacagcta
     3361 tttgcggaat tatcaatgta accccagatt ccttttcgga tggtggtcaa ttttttgttc
     3421 ttgagcaagc gctccagcag gctcgtaaat tgatagcaga aggagccagc atgctagata
     3481 tcggcggtga atcgactcgg cctcggccgg gaagtagcta tgttgagata gaagaggaaa
     3541 tccagcgtgt tgttccagtg attaaagcga ttcgcaagga aagtgatgtc ctcatctcta
     3601 ttgatactcg gaagagtcag gtggcagagg ctgctttggc tgctggtgcc aatctagtca
     3661 atgatatcac tggtcttatg ggtgatgaga aaatggccca tgtggttgct aaggctggtg
     3721 cgaaagtagt cattatgttt aacccagtta tggctcggcc tcagcatcct agttcgctta
     3781 tcttccctca ttttggtttt ggtcaaacct ttacagaaaa agagttagct gactttgaaa
     3841 cattgccaat cgaagaaatg atggaggctt tctttgaacg agcactagcg agagcggcag
     3901 aagctggtat tgcaccagaa aatatcctgt tggatccagg aattggcttt ggtctgacca
     3961 agaaagaaaa tctgcttctt ttacgggacc tggataaact acatcagaag ggctatccaa
     4021 tctttctcgg agtgtcgcgc aagcgatttg tcatcaatat cctagaggag aatggttttg
     4081 aagtcaatcc tgagacagag cttggtttcc gaaatcggga cacggcttcg gctcatgtaa
     4141 ctagtatcgc tgcgagacag ggtgtagaag tggtgcgcgt gcatgacgta gctagtcaca
     4201 ggatggcagt tgaaattgcc tctgccattc gtctggctga tgaagcggaa aatttagatt
     4261 taaaacaata taaataagat gaaagaaatt gaaaacaatc agtggattgc taactaccgg
     4321 acggatcaac cgcattttgg cttggaacga atggtggaac tgttagcttt gcgtggcaat
     4381 ccccatctca aactcaaggt cctccatatc ggagggacta acggcaaggg ctcgactatt
     4441 gcttttttga aaaagatgct agaaaagcta gggctgagag ttggcgtgtt tagctcgccc
     4501 tatctcattc attacacaga ccagattagc atcaatgggg aatcgatctc agaagcgagg
     4561 ctagaagctc tcatggcaga ttatcagtct ttgctggagg gagaagcggt cgccaattta
     4621 cagggcacaa ccgagtttga gattatcaca gccctggcct atgactactt tgcctcagag
     4681 caagtagatg tggccatcat ggaagttggc atgggtggac ttttggatag taccaatgtc
     4741 tgtcagccca ttttgacagg aattacaact attggcttgg atcatgtggc cctacttggt
     4801 gacaccttgg aggccatagc agagcagaag gcaggtatta tcaaacaagg gatgcccttg
     4861 gtaacagggc gtattgctcc agaagccttg actgtgattg accgcattgc ggaagggaaa
     4921 gatgcgccga gacttgccta cgggacagat tatcaggttc gtcatcaaga aagtgtggtg
     4981 acaggggaag tctttgacta tacaagtgct gtcagacaag gtcgcttcca gactagcctg
     5041 cttggtttgt accaaataga gaatgctggg atggccatag ctttacttga tactttttgt
     5101 caagaagatg gtcgagagct agcaagcaat gactttcttg gtcaagcctt ggaagaaaca
     5161 agttggccag ggcgtttgga aatcgtgtca agagatccct tgatgatttt ggatggagcc
     5221 cacaatcccc atgctatcaa ggccttgttg gtaaccttgc aagaacgttt tgcagattat
     5281 cataaggaaa tcctcttcac ttgtatcaaa accaaggcct tggaggatat gttggccttg
     5341 ttgggagcca tgccagatac cgagcttact ctaacacatt ttgcggatag tcgggcgacg
     5401 gatgaaagcg tgctgaaaga ggcagctaag tctagaaatc tcagctacca agattggcat
     5461 gattttctag atcagaattt gacagataaa aaagaagaga aacaaacagt taggattgtc
     5521 acaggttcct tgtatttctt gagccaagtg agggcctatc tgatggagag gaagaacgag
     5581 aatggataca caaaagattg aagcagctgt aaaaatgatt atcgaggctg taggagagaa
     5641 cgctaatcgc gagggcttgc aggaaacacc tgctcgtgta gcccgtatgt atcaagagat
     5701 tttttcaggt cttggtcaaa cagcagagga acatttgtca aaatcctttg aaattattga
     5761 cgataatatg gtggtagaaa aggatatctt tttccatacc atgtgtgaac accacttctt
     5821 gccattttat ggtagagcgc acattgccta cattccagat ggtcgtgtgg caggcttgtc
     5881 taagctagcc cgtacggttg aagtttattc gaaaaaacca caaattcaag aacgtttgaa
     5941 tatcgaagtg gccgatgcct tgatggacta tctaggtgct aaaggagcct ttgttgtcat
     6001 tgaggcggaa catatgtgta tgagtatgcg tggtgttaga aaaccaggca ctgcaacctt
     6061 gacgacagta gctcgtggtt tatttgaaac agataaggac ctccgcgacc aagcttatcg
     6121 tttaatgggg ctataaaaag aatccgcttc aagcggattt ttctagaaag gaatcattat
     6181 ggatcaactg cagattaagg atttggaaat gtttgcctat catggtcttt ttcctagtga
     6241 gaaagaattg gggcagaaat ttgtcgtttc agccatccta tcctatgata tgaccaaggc
     6301 agctacagac ttggatttaa cagcctctgt ccattacgga gaattgtgtc agcagtggac
     6361 gacttggttt caggaaacga gtgaggattt gattgaaacg gtagcctata aactggtgga
     6421 acgtaccttt gagtcttatc ctcttgtcca agaaatgaag ttggaactga aaaaaccttg
     6481 ggcaccggtg catttgtcac tagatacttg ctcggtaacc attcatcgcc gcaagcaacg
     6541 agcctttatc gccctaggaa gcaatatggg agataagcaa gcaaacttga agcaagccat
     6601 tgacaaactg cgagctcgtg gcatccatat tctcaaagag tccagtgtct tagcgacgga
     6661 gccttggggt ggagtggagc aggatagctt tgccaatcaa gtggttgagg tggaaacctg
     6721 gctaccagca caagacttgt tagaaacctt gttagccatt gagtcagagc tgggacgggt
     6781 gagagaagtg cattggggac ctcgtttgat tgatttggac ttgctctttg tggaggacca
     6841 gatcctttat acagacgacc tcatattgcc tcatccttac atagcggaac gcctttttgt
     6901 ccttgagtcc ttacaggaaa ttgcgcctca ttttatccat ccgatattaa aacaaccgat
     6961 ccgcaacttg tatgatgctt tgaaaaaata gaaaaactct agttttcagt tacttgcaac
     7021 tgaaggctag agtttttaaa ataggtcatt ttcttctggg aggaggatag tttctctacc
     7081 gtccatgtct aaaaccagta ctcttggggg ataacgaggg tcgaaaggat ggttaaagtc
     7141 aaaatcaatg gctgtaggga ggtgttgact tgaaaagtgg aaggtaatct ttccttggtt
     7201 attaagcaat tgaaactcga gttcttcttc caattcaaag acatttttta agaaatgatc
     7261 gatgatatac caaaaagagt caatgatgtc atcaggcaag ctggtaacaa tgccaaaact
     7321 agcagaccgc atgtgggtat tggtaaaagc catatctctg tcccctttct tttcccttat
     7381 catacagcaa ataggattaa aaatcaagaa aaggtgattt tttgaaaagg attttaacta
     7441 cagggaaaaa gaaggaaaaa aattcccaaa aatctaccga agttaatagg taaattccca
     7501 aattaacttg attatataac tttcagttac tttgagaagt taccgaaaaa tatttttcat
     7561 atctattgac ttttaggggt gaaatttggt atgatagtag gcggtattgt ttaccccatt
     7621 tgaaaggccc cggaaccttc caaatacttt tcgatgggaa ggaacaccca tcaccgtaaa
     7681 caaaaatcga actatatata ggagaaatca tgaacaaaac aacatttatg gctaaaccag
     7741 gccaagttga acgtaaatgg tacgtagttg acgcaactga tgtaccactt ggacgtcttt
     7801 ctgcagtagt tgctagcgta cttcgcggaa aaaacaaacc aacatttaca ccacacactg
     7861 atacaggtga cttcgtgatt gttatcaatg ctgaaaaagt taaattgact ggtaaaaaag
     7921 caactgataa aatctactac actcactcaa accacccagg tggattgaaa caaatctctg
     7981 caggtgaact tcgttctaaa aatgcagtac gtttgattga gaaatcagtt aaaggtatgc
     8041 ttccacacaa tacacttgga cgcgctcaag gtatgaagtt gaaagtattt gttggagctg
     8101 agcacactca cgctgcacaa caaccagaag ttcttgacat ttcaggactt atctaaggaa
     8161 aggaacaata aagtatgtca caagcacaat atgcaggtac tggacgtcgt aaaaacgctg
     8221 ttgcacgcgt tcgccttgtt ccaggaactg gtaaaatcac tgttaacaaa aaagatgttg
     8281 aagagtacat cccacacgct gaccttcgtc ttgtcatcaa ccaaccattc gcagttactt
     8341 caactgtagg ttcatacgac gttttcgtta acgttatagg tggtggatac gctggtcaat
     8401 caggagctat ccgtcacggt atcgctcgtg cccttcttca agtagaccca gacttccgcg
     8461 attcattgaa acgcgcagga cttcttacac gtgactcacg taaagttgaa cgtaagaaac
     8521 caggtcttaa gaaagctcgt aaagcatcac aatttagtaa acgttaattc gaaagaatta
     8581 ctatacttat acagagcacc tttcggggtg ttcttttttt atactttctt actaaattgg
     8641 tgcaattgac acagttgttg cgactttagt cgcttacaaa tgtggctgca acctgacaag
     8701 gtcagttgcc tcaaaacgtt aatcaataca attatatcaa cgtttcaaag cactcaagag
     8761 tttacctcat gggtgctttt tttatgtttt ttgaaaaagt tcacctcaaa gtttacctta
     8821 ttttcacctt attgttttag agactgtaag gcaaattcac cgacggccat agggactaca
     8881 ttagaagtat tgacataaat ctgtgttgta cctgtgtcgc tatgtattag agcttcagaa
     8941 atggcttcta agctcattcc tgcttgttta gcgagcgttg ctccggtatg acgtagtttg
     9001 tgtggcgtag cgtgtgtaag ctctgggtgt cgttttctga tagttttcat cttattattg
     9061 agatagtcag aatgtaaagg tttattgata ttgcctttgg tatctatgta agtaaagatg
     9121 aattgttctg gattactgat gataccaaac tttgccagtt catatttttg ttgttttttc
     9181 catgaagtca gaagttgaag taagtcatta gaaatagaga aaatgggaaa tgtttccaca
     9241 ataaaacgca tattatcaga tggttaatgc gttttatatt tgggtttgag gcttataaaa
     9301 taggataagc gtactgaaaa cttagatatg acaaacaaaa taaaattttt aaaagctaca
     9361 gagaaacatg gtataatgta tctaaaggag gtagcatatg tccttagata tagataaaga
     9421 aaagatgaca atcatgggaa tagcttttga aaaccgatcc gtttttaaga gtgtttggta
     9481 tgctttaagt acaaatatga ttgaaggatg gcgacctacg gttagtgatg ttgaaaaatt
     9541 acgagatgaa gctcttgctc tggggatgac ttaatgaagc gcaactgtta tgaatcttat
     9601 gactatgttg atccggataa tttatatacc gatccgggtt cttcagttct aagaaataag
     9661 caggaaggaa aaaagcacgc gagcttgaat atcgtatgat tgctagtaag agtttgaaat
     9721 tatttatcaa tcctatctta gttaatagtt taaaagtagt ttgtttcaca actgcataag
     9781 cttagaaaag ttgaaaagtg ataactcttt aataaactct tggtttgtta aaagatattt
     9841 tgtccactac atttttttca attgactatt atttgtcata gatataacag atagaacatt
     9901 gcattcagaa taatgaaaaa cgcatcaaaa atggtgcgtt ctgtttttat gctaccataa
     9961 aaggaggaaa acaaattgaa aatttttaag ggagagtttt atcgaatctc tgtattaaca
    10021 gacaagctag taaggttaga atactctcga actggaaatt ttgaggatag aacgacacaa
    10081 cttatctata atagagattt tggccaagtt tcgttagatt atatcgagac atcaaatgta
    10141 ctagatatta tgacggacta ttttcatctg cactttaata aaggagaatt taacgccgaa
    10201 aatttattta tagaattaaa aggaaatttt gccgtatatg gtagtcgctg gtattttggt
    10261 gaatctattg aaacgttaaa aggaacagct cggactctgg ataaggcaga tggagcaatc
    10321 tcgttagaag atggaattat tagccgaaat ggtatagcct tattggatga ttctcaagga
    10381 tttatttggg atgaacaatc tggttatatt gagagagaaa atcaaattga cctgtatttc
    10441 tttgcctatg ggcatgatta tagaggagca atcagagact tttaccattt gactggttca
    10501 acacccttgt tgccaagata tgctttaggc aattggtgga gtagatattg gccttatacg
    10561 tcggatgaat acttggattt aatagacaga tttgaaacag agaaaattcc attatctatc
    10621 ggtgtgttag atatggattg gcatataact gacattccag ctcgttttgg aagtggctgg
    10681 acaggatata gttggaatag aaacttaata ccaaatccag aacagttatt gcaacaactt
    10741 catgatagaa agttaaaact ctccttaaat gtccatcctg ctgatgggat acgggcttat
    10801 gaagaagctt atcctcaaat cgcaaaacgg ttggggttaa atgtagaact agaagaacct
    10861 gctatttttg atttttttaa tccctctttt agggaagcct actttaaaga tgttcattat
    10921 gagctagaaa agcagggagt agatttttgg tggattgact ggcaacaagg gacacaaggt
    10981 atgctagatc cactttggct tttaaaccat tatcactatc aggatagttg taaaaatgca
    11041 gaaggtggtt tgattttatc aagatatgca ggtcctggta gtcaccgcta ccctgttggt
    11101 ttttcagggg acactattat tagttggaat tccttaagat ttcaacccta ttttacagcg
    11161 acagcatcta atatcggtta tagttggtgg agtcatgata tcggtggaca tatgttgggg
    11221 gattatgacg aagagctaca aactagatgg ctacagtttg gtgtttttag tccgattact
    11281 cgattacata gttctagaag tccttttaat agtaaagaac cttggttctt ttcagaaaca
    11341 acatctaaga ttatgaagaa ataccttcgt ttgagacatc agatgattcc ctatctatat
    11401 accatgaatg taaagacaca tgaggaaggt gccccattaa tcagtccaat ctattatttc
    11461 tatccagaga atgatgagag ctataatgtt ccaaaccaat acttttttgg aacagaactg
    11521 atggtggctc ccattgtaga aaagatggat ttgacattcc aatctgcaaa agtagatgta
    11581 tggttccctg aaggtgaatg gtatgacttc ttttcagaga aaaaatacac aggtggtgtg
    11641 aagttaagtg tttataggga catctcgact acgcctgtgt ttgcaaaaag tggtgcaatc
    11701 attcccttgg ttggttctga gataggtatg ggtgttgatt tacctgaggt tgtagattgg
    11761 tatgtattcc cagggaaaca acattctttt gaaatgcttg aagatcaaaa tggtcaaaga
    11821 tataaaacaa gattatcaat cgattgggaa atgggaatgg tagagttagc attacaagga
    11881 gattctagta tcgttccaag caatagaaaa catagaattc attttaaagg aacgaatgtg
    11941 tctataattg aattgccaaa taagaatgat acagctaaat ttgaatggaa agataataaa
    12001 aggacatctt taaatgacga agtttttaga ctactaaaga cagcttctct tccatatgaa
    12061 ttaaaagata gattgttaaa tcaattcatc aatgccaaaa actctcatga cttaatgaat
    12121 atcttgcatc atcaggataa ggaattgaga gggcgtttgt tggaaatgat atttactagc
    12181 caaaactaat tgaaataatt agaaagtcat ttgtttagtg tatatgaaga ttctgtatgg
    12241 tattcgttat cactggtcac attgaaattg tgaaataagc acctacaaca tagactttac
    12301 aacacttcat gtttcatatc atggggtgtt tttctattta agtcaaagcc aatcaaaaaa
    12361 tctcactatt catcaggtta aaggaaacct aatcatagtg agattgtgaa ttatagtaga
    12421 gtttgtatcg cctcttcaat ttgttttggg tctgtttttg aagaaaagcg ttcaaagact
    12481 tgcccatctc gaccgatgag aaacttagcg aaattccatt cgactcgttt tcctagtggg
    12541 ccagatttct ggtcttttaa ccagacatag agagggtctg cttccttacc gttgacctta
    12601 atcttggcaa aacgtgggaa ggtggtttga aaatgtaggc tacagaaggc gttgatttcc
    12661 tctgcgctgc cgggtgcttg tcccataaac tgattgcaag ggaaatccaa tatttcgaag
    12721 ccctgatctt gatagcgttc atagagttct tgaagtccct ggtactgggg cgttaaacca
    12781 catccagtag cagtgttgac aatcaagaga actttgccac gatagctatc caagggagtt
    12841 gcttggttgt tttggttcaa aacggaaaaa tcataaagtg aagtcatgag ggcctctttt
    12901 ctttttctac attgtaacag tttttacttt tttcgtcgag tcggatggaa gatattgttg
    12961 aagaataagc cctcaatatg atagaaatcg tttacaaata actttaaaaa tggtatgatt
    13021 taactaaacc ggtttactta gttgctataa atctctaatt tgagatttag ttcctattga
    13081 ttttacaata tgtttattgg agtgtataca tgcaaacaaa aacaaagaag ctcattgtga
    13141 gtttgtcttc acttgtttta tcaggatttt tattaaacca ttatatgaca gttggagcgg
    13201 aagaaacgac tacgaatacc attcagcaaa gccagaagga agttcagtat cagcaaaggg
    13261 atacaaaaaa tttagttgaa aatggtgatt ttggtcagac ggaggacgga agcagttcgt
    13321 ggacaggaag caaagctcag gggtggtcag cttgggtaga ccagaagaat agttctgcag
    13381 atgcctcaac tcgagtcatt gaggctaagg atggggctat cactatctca agccatgaga
    13441 aattaagggc agcgcttcac cgtatggttc ctattgaagt taagaaaaag tataaactgc
    13501 gtttcaagat taaaacagat aataaagtcg ggattgccaa agttcgtatc attgaggaaa
    13561 gtggtaagga caagcgattg tggaattctg caacgacgtc aggaacaaag gactggcaga
    13621 ccattgaagc agactatagc ccgactttag atgttgataa aatcaagctg gagttattct
    13681 atgaaacagg aactgggact gtttccttta aggatattga gctggtagag gtagcagccc
    13741 agctttctga ggattctcaa acagataaac agcttgagga aaagattgat ttaccaattg
    13801 gaaaaaaaca tgttttttct cttgcggact atacttataa ggtagaaaat cctgacgttg
    13861 cttcagtcaa aaatggaatt ttagaacctc ttaagggagg gacaaccaat gtcattgtca
    13921 gtaaagatgg caaggaagtg aaaaagattc ctttgaagat tctagcctct gttaaggata
    13981 catacacaga ccgtttggat gactggaatg gcatcattgc tgggaatcaa tactatgatt
    14041 ctaaaaatga acagatggcc aaattaaacc aggaattgga aggaaaggta gctgatagcc
    14101 tatccagtat ttcaagtcag gcggaccgca cctatttgtg ggaaaaattt tcaaattata
    14161 aaacgtctgc aaatctgact gccacttatc ggaaattgga ggagatggcc aagcaagtga
    14221 ccaatccttc ttctcgttat tatcaagatg aaactgtcgt tcgaacagtc agggattcca
    14281 tggaatggat gcataaacat gtctacaata gtgaaaagag cattgttggg aactggtggg
    14341 attatgaaat cggtacacct cgtgccatca acaatacctt gtctctgatg aaagaatact
    14401 tctctgatga ggaaattaaa aaatatacag atgtgattga aaaatttgta ccagatcccg
    14461 aacatttccg aaagacgact gataacccat ttaaggctct aggtggaaac ttagttgata
    14521 tgggaagagt aaaagtaata gctggtttac tgcgtaagga tgatcaagaa atttcttcta
    14581 ccattcgctc gattgagcaa gtgttcaagt tggtagacca aggtgaaggt ttttatcaag
    14641 atggatccta tatcgaccac accaatgttg cctatacggg tgcttatggg aatgttttga
    14701 ttgatggcct gtctcaactg ttgccagtca ttcaaaagac caagaatcca atcgataaag
    14761 ataaaatgca aaccatgtac cactggattg ataaatcgtt tgctcctttg ctggtgaatg
    14821 gagagctgat ggatatgagt cgtggacgct cgatcagtcg tgcaaatagc gaggggcacg
    14881 tggccgcagt agaagtacta agagggattc accgaatagc ggatatgtct gaaggagaaa
    14941 ccaaacaacg tttgcagagt cttgtgaaga ccattgttca atcggatagt tattatgatg
    15001 tctttaagaa tttgaagact tataaggata tcagtttgat gcaatccttg ttaagtgatg
    15061 caggagtcgc aagtgttcca agaccaagtt acctatctgc ctttaacaag atggataaaa
    15121 cagccatgta caatgcagag aaagggtttg gatttggctt gtcactcttt tccagtcgta
    15181 ccttgaatta cgaacacatg aacaaggaaa ataaacgtgg ttggtatacg agtgatggga
    15241 tgttctatct ttacaatggc gatttgagtc actatagcga tggctactgg ccaacagtta
    15301 atccatataa gatgcctggt acaacagaga cggatgctaa gagagcggat agcgatacag
    15361 gtaaagtttt accgtctgct ttcgttggaa cgagcaaact agatgatgcc aatgcgacag
    15421 caaccatgga tttcaccaac tggaatcaaa cattgactgc tcataagagc tggtttatgc
    15481 tgaaggataa gatcgccttt ttaggaagca atatccaaaa cacttcaaca gatactgctg
    15541 caactacaat tgaccagaga aaactggaat caagtaatcc atataaagtc tatgtcaatg
    15601 ataaagaagc ctcccttaca gaacaagaaa aggattatcc tgaaacccaa agtgtctttt
    15661 tagaatcgtc cgattcgaaa aagaatattg gttacttttt ctttaagaag agttcaatca
    15721 gtatgagtaa ggctttgcaa aagggagcct ggaaggatat caatgaagga cagtcagaca
    15781 aggaagttga aaatgaattt cttacgatta gtcaggctca taagcaaaat ggagattctt
    15841 atggctatat gctcatccct aacgtgggtc gtgccacctt caatcaaatg ataaaaaagt
    15901 tagaaagtag cctcatcgaa aataacgaaa cccttcagtc tgtttatgat gccaaacaag
    15961 gagtttgggg cattgtgaaa tatgatgatt ctgtctctac tatttccaac caattccaag
    16021 ttttgaaacg tggagtctat accattcgaa aagaagggga tgaatataag attgcctact
    16081 ataatcctga aacccaggaa tcagctccag atcaggaagt ctttaaaaag ctagagcaag
    16141 cagctcagcc acaagtacag aattcaaaag aaaaggaaaa atctgaagag gaaaagaacc
    16201 attcggatca aaagaatctc cctcagacag gagaaggtca gtcaatcttg gcaagtctag
    16261 ggttcttgct acttggggca ttttatctat tccgtagagg aaagaacaac taatttgttc
    16321 atcatctaga tgaattacat gaaactgtgg aaaatagaaa aaatccgaag aaggccaaat
    16381 atcttcggat ttttctttta aaaaagataa gttgatactt atctttttta cttcattatg
    16441 tgactatagc gttttgaaac tagaatagga cgttataact actaaaagat ttctataaat
    16501 tcatttgatt ttcctaatca atttgttcgt atcctatttc actccactat attagtccgt
    16561 ctcgctccgt gaggtgcgag acaaaacaac cacccgctat gcgggtgcgc gtcgaaggtt
    16621 ataccaaaaa aactctaaac gcgatacaat aaaggtgttc aatccaattg taaagcgaaa
    16681 ggagaaaaaa tatggcacaa aaggctcata gtttatctca cacaaagtgg aggtgttcta
    16741 tcacattgtg ttcaccccta agtatagacg aaaagtcatc tataatcaat atagaagtag
    16801 tttaggcgaa atatttcatc gcttgtgtag ttataaaggt gttgaaatga tcgagggtca
    16861 cttaatgcca gaccatgtac acatgttagt cagtattccg tcgaggctaa gtgtttcaag
    16921 ttttatgggg tatttaaaag gcaaaagtgc actcatgatg tttgacaaac acgccaatct
    16981 caagtacaag tttgggaatc ggcatttctg ggcggaaggt tattatgtaa gtccagtagg
    17041 gcttaatgaa gccacactta agaaatatag tcaagattaa agaaattatc cagtggatga
    17101 tttcttcacg agtatgaaaa tttgagaact agtaaagtat gatatatagt aaaatgaaat
    17161 aagaacagga caaaatcgat caggacagtc aaatcgattt ttaacaatgt tttagaagta
    17221 gaggtgtact attccagttt caatctacta tagcactaga taaattgagt gtaaaagaat
    17281 atgaggatcc ctttagggat agtggtaagt aataccaaag tatcttaaag aggcaagtga
    17341 cgagtcaaga gcaataaggc ttgaacaacg tgaaagccag cgtctttagg cgctggctgg
    17401 taatttgagc ttatagctct gggataaacc acccgtttga cgggtggtta tgattttatc
    17461 tgagtgtaat atactgttgg gcaatctcgc tgatgcggtc aaagttgcct tgggaagcga
    17521 gtttattgag ttcgccacca attccaacgg catctgcacc agcagcgaac cattgaggga
    17581 tgttgtttag gccgactcct ccggttacca ttacggaaac ttgtgggatc ggtgccttga
    17641 ctgcagagat atatgctgga ctgagagtac tacctgggaa gagtttgatg atttcactac
    17701 cggcttcaag tgcagtcgtg atctctgtga gggtaataca gcctggaatg tacggtgtgc
    17761 tgtagagatt gcacattttc gcagtttcag catggaaaga tggagaaaca acgtaatttg
    17821 ctccggctag aatggcatct ctagcagtta cggcatcaag cacagtacct gcaccgatac
    17881 aaacactctg atcgtcctga tacaagtcta caagttcctt gatgatttgc cctgcatact
    17941 gattggtata ggcgatttca atagctttga taccgccctt gatacaagca atcgaggctt
    18001 gcagtccttc ttcctttgta tttcctcgaa tgacagcgac aattttctgt ttttttagtt
    18061 caataatcgt atctgatttg gtcatgtaat tctcctaacg aatgatatct tgtgcatttg
    18121 ccagtaaatt ttcaatacta gttgcggaag tggagagatg gtctcctgga agagtgcatt
    18181 tgagagttgc gctcgcaact gcaaagtcaa tggtagtttt tagggaggaa tgatggagta
    18241 gttggtaaag ggcaccagat ataaaggcat ccccgctacc aattcgttga tagactgcag
    18301 tttttagttg gacagactct tcaaatagtt cttctagagc ataggcttga tagacatttt
    18361 tgtcttgctc atcactagag cggagggtat ggaaaatggc cttgaaacca taggcttctt
    18421 ttaaaagtcg catgcgattt tccacctctt ctaggctagc actgtctctt ggaaacatct
    18481 ctagattttg gtcatcaatc atgagaggat caataccgaa gcaatagtca gtaaaacgtg
    18541 caaacttaga aaattcatac ttggcttcta ggactgaaat catctttgtt ctcagattga
    18601 gatccattga aacgacaatt cctcggcgct tggcttcttc caagagtagg agaaggatcg
    18661 cacggacctc ttgaccgata gctacggtga ttccactaaa atgaaaatgg ctaatcccct
    18721 gaaagagaga atccatatct agcatgtttg gccgaatctg gctgatactc gtatgcttac
    18781 gatcgtagaa aacttcactt tgacgacaac caaagccgtt ctccaaatag tagaggccga
    18841 ttcgatcgcc aagccgacag attgaactgg tatcgatttg gtgctgtttc aagaatgtga
    18901 gaaaacgatc tccaatctcg ttggcaggga gtgcggtaaa gactttcgtt gagataccca
    18961 gggcttgcaa attacaagcg atgttaattt ctgatccgcc aaaataagtc gaactggcaa
    19021 catgatcgcc gatactggtg gcatctaatg gtgagattcg aattaatggt tcgccaaaca
    19081 gtaatatttt cgtcatccat tagttccttt ctggtttaga atagctttac ttagctaata
    19141 cttctttcaa ataagcagcg acttcatcat cttggcagtt ttcaaagaag tattcttgga
    19201 agtatttgcc agagatggtg tctttgagga agtcttggtc gattgtttta aggatggtca
    19261 tcaaatcatt gtgggtgatt tgtttcacct cgtttaagat acgagcgttg cgttgttcag
    19321 ggattacacg ttctcttggg tagccaccgc ccatttcttc agcaaataag cgttcaaaca
    19381 tcaatttgag ggtcagttct gccccccagc caaatccttt ggcataaggg atagacaagg
    19441 cgttaccacc attgatttga gaataaaggt aagcgtcagt tgggtccact gctagaccac
    19501 agacaacacc agggaagctg tttaaagcaa gcatagcccc tacacccgta ccacagccgg
    19561 taacaacaaa gtcaactgcc tttgtattta aaaggatggc agccattagt ccgttctgca
    19621 cataagttaa ttgactttct ccttcttctc cacgcatacc atagttaaat aattggtagt
    19681 cttttttatc tgtcgcttct tttagactat cgtaaataat gtgattcttg ctagcttgac
    19741 tattttcatt gattaatgcg attttcataa gatcttcttt ctatttttac tcaggttgtt
    19801 ttccgatgta ggctaagata ccgccatcta catataggat gtggccattg acaaaattgc
    19861 tggcatcact agcgagaaag acagcagggc ccatcaaatc ttcaggattt ccccaacgtg
    19921 cagcaggtgt ttttgcaatg atgaactggt caaatgggtg gcgagaacca tcttcttgca
    19981 actcacgaag aggtgctgtt tgaggagtgg caatataacc cggtccaatt ccgttacatt
    20041 ggatattggc tccaccgtat tcagacgcaa tgttgcgggt caacattttc aagccccctt
    20101 tagcagcagc ataagcgcta actgtttcac gtcccagttc gctcatcatc gaacaaatat
    20161 tgataatctt tccatgccct ttctttatca tagaaggaat aactgccttt gaaacgataa
    20221 atggtgcgtt taagtcaata tcgatgacct tacggaaatc agcggcgctc atttcgcaca
    20281 ttggaactcg gcggataata ccagcgttat taacgaggat gtcaatgaca ccaatctctt
    20341 gttcgatttg cttgaccatg gcttggatac cgtcctcgtc tgtcacgtca cagacatatc
    20401 catgggcttc gatgccaact tcacgataag ctttaatccc tttattgacc agatcttgat
    20461 tgatatcgtt aaagacaata gtagcgccgg cttcagcgta ggatttggca atagcaaatc
    20521 caattccata agaagcgccg gtgatgaggg caatttttcc ttttaaagaa aactgctcaa
    20581 ttgagaatga tgtatttgtc atttgatact tcctttcaaa tttgttaaga cttctctata
    20641 accaaattat attctagtaa accggttttg tcaataaata aatattttta tttataaaaa
    20701 gatttatttt ccttaaaaat ggaatatttt gaaatttctg tctattaaaa tgattaaaaa
    20761 gataatgaaa gcgctaatat ttttttaaaa aaactattga caagataaaa ataaaggatt
    20821 acaatgggga atataaagta aaccggtaaa cctaaacaag aaaggagaaa agatgaaaat
    20881 tgtacttgta gggcatggac attttgctac agggatttat agttctttac aattgattgc
    20941 aggcaatcaa gaaaatgtgg aggcaattga ctttgtggaa ggaatgtcag cagatgaact
    21001 caagcaaaaa atcttacttg caatttcaaa tgaagaagaa gttttaatcc taagtgatct
    21061 cttgggagga tcgccattca aggtttcttc taccataatg ggagaaaatc cagccaaaac
    21121 aatgaatgtt ctctcgggtt tgaacttagc catgttaatg gaagcagtct ttgctagaat
    21181 ggctcatagc tttgatgagg ttgttaataa atcagtagtg gcggcccagg gcggagtcgt
    21241 aaatggtaaa gaattgtttt caacggatgc agaggaagag gaagaagatt tcgaatcggg
    21301 tatttaaagg gtaaaagaat gataaaaaag gttacgattg aaaaaataaa atcgcctgag
    21361 cgcttcttag aagtaccact tctgacgaaa gaagaagtcg gccaggcaat cgataaggtt
    21421 attcggcagt tagaactcaa ccttgactat ttcaaggaag atttcccgac gccagctacc
    21481 tttgataatg tctatccaat catggataac acggaatgga ccaatggttt ctggacagga
    21541 gaactgtggt tggcttatga atacagtcaa caggatgcat ttaaaaacat cgctcataaa
    21601 aatgttcttt ctttcctgga tcgtgtcaat aagagagtag aattggatca ccatgatctc
    21661 ggcttcttgt acacaccgtc ttgtatggct gaatataaga taaatggaga tggagaggct
    21721 agagaagcag ccttgaaagc tgcagataag ttgattgaac gctatcaaga aaaaggtggt
    21781 tttattcaag cttggggaga cttgggcaag aaagagcatt accgtttgat tatcgactgc
    21841 ttgctcaata tccaactctt attctttgct tatcaagaaa caggcgatca aaaatactac
    21901 gatattgcag aaagccattt ctatgcttca gctaataatg taatccgtga tgacgcttcg
    21961 tccttccaca ccttctattt tgatcctgag acaggtcaac cctttaaagg tgtaacgaga
    22021 caagggtata gtgatgattc atgctgggca cgtggtcaat catggggagt ctatggtatt
    22081 cctttgactt atcgtcactt aaaagacgag tcctgctttg acttgtttaa gggtgtgacc
    22141 aattatttct tgaatcgtct gccaaaagat catgtgtctt attgggattt gatttttaat
    22201 gatggtagtg atcaatcacg agattcttca gcaacagcta tcgccgtctg tgggattcat
    22261 gaaatgctaa aacatctccc agaggtggat gctgacaaag atatttataa acatgctatg
    22321 catgccatgc ttcgttcctt gatcgaatat tatgcaaatg atcaatttac ccctggtggg
    22381 acaagtctcc tccacggtgt gtactcatgg cattcaggta aaggagtgga tgaaggcaat
    22441 atctggggtg actactatta cctagaagcc cttatccgtt tctacaaaga ctggaaccta
    22501 tattggtagg aggagaaata tgacaatgcc aaatattatt atgacccgta tcgatgaacg
    22561 gttgattcat ggacaaggac aactttgggt aaaataccta ggttgtaata cggtcattgt
    22621 tgccaatgac gaagtaagca cggacaagat gcaacaaact ctgatgaaaa cagttgtgcc
    22681 agactcagtt gccatgcgtt tcttcccttt gcaaaaggtg attgatatca ttcacaaggc
    22741 taatcctgct caaacgatct ttatcgttgt aaaggatgta aaggacgctt taaccttggt
    22801 agaaggtggt gtccctatca aagaaatcaa tattgggaac attcacaatg cccctggtaa
    22861 agagcaagtg acacgctcca tcttcctggg tgaagaggac aaggcagccc tcaaggaatt
    22921 aagccaaact catcaagtaa catttaatac gaaaacaact ccaacaggaa atgatggagc
    22981 tgttcaagtc aacattatgg actatattta aaagaggaga tcgttatgtc gattaatgta
    23041 tttcaagcga ttttaattgg attatggaca gctttctgtt ttagtggaat gctgttagga
    23101 atttacacca atagatgtat tgttctgtca tttggtgtcg gaattattct aggtgatctg
    23161 cctactgctc ttgcaatggg agctattggt gaattggctt atatgggatt cggtgttggt
    23221 gctggaggta ctgttccacc aaacccaatc ggacctggta tctttggtac cttgatggct
    23281 atcactagtg ctggtaaagt cagtccagaa gcggctcttg ccctctctac tccgattgct
    23341 gtggcgattc aattcttaca aactttcgcc tacactgtac gtgctggtgc gcctgaaacg
    23401 gcgatgaagc acttgaaaaa ccataatttg aagaaattca agttcactct aaatgcaaca
    23461 atttggttgt ttgcctttat tggatttacc ttgggttgct tgggtgccct ttcaatggat
    23521 accttgttga aactcgtaga ctacattcca ccggtattac ttacaggttt gacagttgct
    23581 ggtaaaatgc tcccagctat cggttttgcg atgatcttgt cagtgatggc taagaaagag
    23641 ttgattccct ttgtcttgtt gggatatgtt tgtgcagctt atctaaacat cccaacaatt
    23701 ggtattgcaa ttgtaggtac tatctttgct ttgattgaat tttataacaa gccaaaaaca
    23761 gcggatcatg tggtagagga ggaagcacac gatgactgga tctaacaaat taacaaaacg
    23821 tgattatctt aaaacgtctt tgcgggcatt cttttgtcaa aatggattta actatagtaa
    23881 ctatcaaggg ttgggatatg ccaatgtaat gtatcctgct ttgaaaaaac actatggaga
    23941 ggatcaggaa ggtttctacc aagccttgga agaaaactgt gaattctata ataccaatcc
    24001 acacttcctg ccttttatta ccagcttgca ccttgtaatg ttggaaaatg gccgtccggc
    24061 aaaagaaaca cgtagcatca agatggcctt gatgggacca ttggcaggta ttggggattc
    24121 tctttctcaa ttctgtttag ctccattgtt ctcaaccatc gcagcttcgt ttgctcaaga
    24181 aggcttggtt gtcggtccaa tcttgttctt ccttgcgatg aatacgattt taacagcgat
    24241 taaattgtca actggtctgt atggatacaa actaggaaca actgtgattg ataaactaag
    24301 cgaacagatg gcaacgattt ctcgtattgc caatattatc ggtgtaaccg taattgctgg
    24361 tttggcagcg acatctgtta agattatggt gccgattacc tttgctgcag gggaagttaa
    24421 agcagacgct aaacaaagta tcgtaagtat tcagggaatg cttgataagg ttgctccagc
    24481 tcttctacca gccctattta cacttttagt ttactacttg atcaaagaaa agaaatggac
    24541 aacatataaa ctcgttattt taacagttat catcggaatt atcggaagct ggcttaagat
    24601 tatagcttaa tggatacaac attgttttat ggaatagtga ttgtcttggc agtgagtccc
    24661 cttttactgt caagctttca ttctattcgt cagcaaaagt tgcttcgcaa acagatggag
    24721 caacgacaag agtatttagc ttctttaaca tctggtgatg aagtgttgtt attgtctgga
    24781 attcatggaa aaatcatttc tatcaaagat gacttggtct ccttgcagat tgcaaaaggt
    24841 gtggtcatct atgtagaaaa ggaaagtgta atgggaaaga caaaagaact gctttttaag
    24901 tagttctttt tctggtaata gataatgaaa gaagaaagaa gacaattttt tgaaagagtc
    24961 gatggaaacc aatgtcgtga ttatatcttg tcccactgtt caaaagacta tgagaaagtc
    25021 aagagttccc ttgaacgctt gatggacaat cgttttatgt ttgatagtcc ttgggatatg
    25081 gagccttgtt caaaaatcca tcaaatccag ccgatggtat gggatcaagt atttgaagat
    25141 gatccagaat ggtcttatat gctcaatcga caagaatatc tcttgcagtt tatgataggg
    25201 tatctggtag aaggagataa ggactatatt caaaagtgca agttctttct atttgattgg
    25261 attgagcagg tgagagaatt ttctcctcaa tccttgatga ctagaacctt ggatacgggt
    25321 attcgttcct ttacttggtt gaaactactc ttgctcctct tgaaatttga cttgctagag
    25381 gagaaagaac tagagaaaat tttggtcagt ctagaaaagc agattgactt tatgaaaagc
    25441 tactatcgcg ccaagtacac ccttagtaac tgggggattt tacaaacaat tccgatgctt
    25501 gctatctatc atttcttttc agataagatg gacctagaag aagcttacca ttttgcttca
    25561 gaggagttga aacagcaaat tgagacacag attttaggag atggaagcca gtttgaacag
    25621 tcgattctct atcatgtaga ggtttataaa gccttgctgg atttgtgtct cttgcttcca
    25681 gacttgcaag atagttacca agagttgctg gaaaagatgg cgacctatat tcaaatgatg
    25741 acaggcttag atggacggac tttggctttt ggtgatagcg attctacaga aacgacagaa
    25801 atgttgagcc tgtctgctgt ggttttgaac aaggaagacc ttcttaacgg tctggatgtt
    25861 aaagtcgatt tgcttagcct cttgttcctg gggcgagaaa aggtcaagcg actgcaggaa
    25921 tttgaaagga gagcttggca gcctaagtcc atgatctttg aagactctgg acatgtctgc
    25981 attaaggatg aacatcgtta tctatttttc aaaaatggtc cgctaggaag tgcccatagc
    26041 catagtgacg agaatagttt ttgcttacag tatcaaggcc aacctatttt catagatgct
    26101 gggcgttatt cttatcggga gatatatgaa cgttatctct taaagagtgc ttggagtcat
    26161 tcgacctgca ttgtagatgg gaaagctccg gaaagaatca cgggatcctg ggaatatgaa
    26221 tactatcctc actccctgtt ttgtcaccat aaagaaaggg agggagtgca ttatattgag
    26281 ggggcttatt ggtcagcaga acctgatttg ccttatcttc acaagagaaa aatcctcatg
    26341 ttggtagagg atgtctggct cttggtagat gacatcaggt gtcaaggtca gcatgaggcg
    26401 ttgattcagt ttatccttga caaggatgtg acctatcaag atgggaaaat caatcagttg
    26461 agactatgga gtgaagttga ttttgatttg gaagatacca tcatttctcc taaatacaat
    26521 gagcttgaaa gaagtagcaa gctcaccaag cgccaattct ttgagaatca gatgctggat
    26581 tataccatca ttgcgcatga gagttttgaa atcatccgtc attctgtcta ccagacagat
    26641 gatcgtgaag tggaaaatgc tctggctttt gaagtgaaaa atgacgaaac agacaagctg
    26701 attctgttat taagcgagga tattggtgta ggtgaaaaat tgtgcctcgt tgacggaaca
    26761 aaaatgcgtg gaaaatgtct agtatatgat aaaataaatg agagaatgat tcgcttgcag
    26821 tgctagaaat aggcattttg aatagtgaat atgttataat aagtattagt aggaggtgtt
    26881 ttagattgga gaagaaactg accataaaag acattgcgga aatggctcag acctcgaaaa
    26941 caaccgtgtc attttaccta aacgggaaat atgaaaaaat gtcccaagag acacgtgaaa
    27001 agattgaaaa agttattcat gaaacaaatt acaaaccgag cattgttgcg cgtagcttaa
    27061 actccaaacg aacaaaatta atcggtgttt tgatcggtga tattaccaac agtttctcaa
    27121 accaaattgt taagggaatt gaggatatcg ccagccagaa tggctaccag gtaatgatag
    27181 gaaatagtaa ttacagccaa gagagtgagg accggtatat tgaaagcatg cttctcttgg
    27241 gagtagacgg ctttattatt cagccgacct ctaatttccg aaaatattct cgtatcatcg
    27301 atgagaaaaa gaagaaaatg gtcttttttg atagtcagct ctatgaacac cggactagct
    27361 gggttaaaac caataactat gatgccgttt atgacatgac ccagtcctgt atcgaaaaag
    27421 gttatgagca ttttctcttg attacagcgg atacgagtcg tttgagtact cggattgagc
    27481 gggcaagtgg ttttgtggat gctttaacag atgctaatat gcgtcacgcc agtctaacca
    27541 ttgaagataa gcatacgaat ttggaacaaa ttaaggaatt tttacaaaaa gaaatcgatc
    27601 ccgatgaaaa aactctggta tttatcccta actgttgggc cctacctcta gtctttaccg
    27661 ttatcaaaga gttgaattat aacttgccac aagttgggtt gattggtttt gacaatacgg
    27721 agtggacttg cttttcttct ccaagtgttt cgacgctggt tcagccctcc tttgaggaag
    27781 gacaacaggc tacaaagatt ttgattgacc agattgaagg ccgcaatcaa gaagaaaggc
    27841 aacaagtctt ggattgtagt gtgaattgga aagagtcgac tttctaaaat gaaggaaaat
    27901 gacttgcaat ctctgttaag aaataaaata atcccaccta gaacaagcta ggtgggatta
    27961 tttgcctatg aaatgagaaa ttatgggagc aagctcctaa atcaactgtt tttgatctac
    28021 ttctttaact acttgataaa agttatagaa gtaggccaaa cttgaaatga tggttacgac
    28081 taggaatatt gaaaatttcc attggacagg gttgcttaaa agttgtggaa aggatatgag
    28141 gagaaagaag agggctgcgt tgaggacagg tatccgtttt gattgtattt tctcaagtcc
    28201 tttattgagc gcaggaagaa agaggagtag gagtagtaaa actgtatgag aaacagctcc
    28261 tgaaataagg gcgaagaaaa ggaaaatact gataaaaaca tgaatgatca gtagtctagc
    28321 tagtgatttc ataaggcacc tcctaatcct ggtctttttt agctcttgca atacgaagtg
    28381 agtcgacaat atgtatcatc actccgaaaa agaaagctcc cagtatagtt ttaaaaatat
    28441 gttttgtatt tagaagagaa ctgataaaat ttggattttc acttgttagg gtatcaatga
    28501 gtggaattat aaaaaatatc actgttccat aaattgaacc tgctttcaga ccaggataac
    28561 gtaactgttt cttttctttt ttcatgagtt tcctcctaat cctcatcttg atttttctta
    28621 gtttttgcaa tgcgacggga gatgaggaac tgtatgctcg ctccgaagaa aatagaaccg
    28681 agaatgcttg atacaccatt tcttatagtg agaagagaat gaaaatagtc ctgaccttca
    28741 tctatgagta tactgagaag aggagttata aaaaacatcc atagaccaaa gaacaaacct
    28801 gctttaagac ctaggtagtg tagttgcttg ctttctttct cattcagcat atctggttca
    28861 atgactgtga tgcctgtttt tttcatttgg taggtgacat agccagcagc gatgagggca
    28921 atcactaaaa tcagaggagg atagattaga gccacttctt gagggtattt ataggccaga
    28981 aggagtggaa taagatttcc gaaaatcatc agataaaaga ggatgataaa gacttggttc
    29041 ccaatactat cggcctcacg ccgtttgtat tcgtcaaggg gaccagaaat accgtatgtg
    29101 cgtttgatca gtttttcagt gaaggtttct tttttcatga gtttgctcct tttttaaaaa
    29161 tcttcctccc aaaagagact gttgaggtca gtttggaggc tgcgggcgag attgagacag
    29221 agttccaagg ttggattgta cttgtcgttt tcaatcatat tgatagtctg tctcgagaca
    29281 ccgatatcct tggcgagttc gagctgggaa atacccaatt ccttgcgaaa ttctttcaca
    29341 cgattcatct gatctccttt ttgatttatg tcgtatatat ttgactttat tatagtcttt
    29401 taaacataaa gtgtcaagta tttttgacat attttttgaa gaaatagtag tctccttgtc
    29461 ctatttgtct gacaagtgca agctggtcgg atttgtggta aaatagataa gatatgacaa
    29521 aagaatttca tcatgtaacg gtcttactcc acgaaacgat tgatatgctt gacgtaaaac
    29581 ctgacggtat ctacgttgat gcgactttgg gcggagcagg acatagcgag tatttattaa
    29641 gtaaattaag tgaaaaaggc catctctatg cctttgacca ggatcagaat gccattgaca
    29701 atgcgcaaaa acgcttggca ccttacattg agaagggaat ggtgaccttt atcaaggata
    29761 acttccgtca tttacaggca cgtttgcgcg aagctggtgt tcaggaaatt gatggaattt
    29821 gttatgactt gggagtgtct agtcctcaat tggaccagcg tgagcgtggt ttttcttata
    29881 aaaaggatgc gccactggac atgcggatga atcaggatgc tagtctgaca gcctatgaag
    29941 tggttaatca ctatgactat catgatttgg ttcgtatttt cttcaaatac ggtgaggata
    30001 aattctctaa acagattgcg cgtaagattg agcaagcgcg tgaagtgaag ccgattgaga
    30061 caacgactga gttagcagag attatcaagt tggtcaaacc tgccaaggaa ctcaagaaga
    30121 agggtcatcc tgctaagcag attttccagg ctattcgaat tgaagtcaat gatgaactgg
    30181 gggcggcaga tgagtccatc cagcaggcta tggatatgtt ggctctggat ggtagaattt
    30241 cagtgattac ctttcattcc ttagaagacc gcttgaccaa gcaattgttc aaggaagctt
    30301 caacagttga agttccaaaa ggcttgcctt tcatcccaga tgatctcaag cccaagatgg
    30361 aattggtgtc ccgtaagcca atcttgccaa gtgcggaaga gttagaagcc aataaccgct
    30421 cgcactcagc caagttgcgc gtggccagaa aaattcacaa gtaagaggga aaaagatggc
    30481 agaaaaaatg gaaaaaacag gtcaaatact acagatgcaa cttaaacggt tttcgcgtgt
    30541 ggaaaaagct ttttactttt ccattgctgt aaccactctt attgtagcca ttagtattat
    30601 ttttatgcag accaagctct tgcaagtgca gaatgatttg acaaaaatca atgcgcagat
    30661 agaggaaaag aagaccgaat tggacgatgc caagcaagag gtcaatgaac tattacgtgc
    30721 agaacgtttg aaagaaattg ccaattcaca cgatttgcaa ttaaacaatg aaaatattag
    30781 aatagcggag taagatatga agtggacaaa aagagtaatc cgttatgcga ccaaaaatcg
    30841 gaaatcgccg gctgaaaaca gacgcagagt tggaaaaagt ctgagtttat tatctgtctt
    30901 tgtttttgcc atttttttag tcaattttgc ggtcattatt gggacaggca ctcgctttgg
    30961 aacagattta gcgaaggaag ctaagaaggt tcatcaaacc acccgtacag ttcctgccaa
    31021 acgtgggact atttatgacc gaaatggagt cccgattgct gaggatgcaa cctcctataa
    31081 tgtctatgcg gtcattgatg agaactataa gtcagcaacg ggtaagattc tttacgtaga
    31141 aaaaacacaa tttaacaagg ttgcagaggt ctttcataag tatctggaca tggaagaatc
    31201 ctatgtaaga gagcaactct cgcaacctaa tctcaagcaa gtttcctttg gagcaaaggg
    31261 aaatgggatt acctatgcca atatgatgtc tatcaaaaaa gaattggaag ctgcagaggt
    31321 caaggggatt gattttacaa ccagtcccaa tcgtagttac ccaaacggac aatttgcttc
    31381 tagttttatc ggtctagctc agctccatga aaatgaagat ggaagcaaga gcttgctggg
    31441 aacctctgga atggagagtt ccttgaacag tattcttgca gggacagacg gcattattac
    31501 ctatgaaaag gatcgtctgg gtaatattgt acccggaaca gaacaagttt cccaacgaac
    31561 gatggacggt aaggatgttt atacaaccat ttccagcccc ctccagtcct ttatggaaac
    31621 ccagatggat gcttttcaag agaaggtaaa aggaaagtac atgacagcga ctttggtcag
    31681 tgctaaaaca ggggaaattc tggcaacaac gcaacgaccg acctttgatg cagatacaaa
    31741 agaaggcatt acagaggact ttgtttggcg tgatatcctt taccaaagta actatgagcc
    31801 aggttccact atgaaagtga tgatgttggc tgctgctatt gataataata cctttccagg
    31861 aggagaagtc tttaatagta gtgagttaaa aattgcagat gccacgattc gagattggga
    31921 cgttaatgaa ggattgactg gtggcagaat gatgactttt tctcaaggtt ttgcacactc
    31981 aagtaacgtt gggatgactc tccttgagca aaagatggga gatgctacct ggcttgatta
    32041 tcttaatcgt tttaaatttg gagttccgac ccgtttcggt ttgacggatg agtatgctgg
    32101 tcagcttcct gcggataata ttgtcaacat tgcgcaaagc tcatttggac aagggatttc
    32161 agtgacccag acacaaatga ttcgtgcctt tacagctatt gctaatgacg gtgtcatgct
    32221 ggagcctaaa tttattagtg ccatttatga tccaaatgat caaactgctc ggaaatctca
    32281 aaaagaaatt gtgggaaatc ctgtttctaa agatgcagct agtctaactc ggactaacat
    32341 ggttttggta gggacggatc cggtttatgg aaccatgtat aaccacagca caggcaagcc
    32401 aactgtaact gttcctgggc aaaatgtagc cctcaagtct ggtacggctc agattgctga
    32461 cgagaaaaat ggtggttatc tagtcggggt aaccgactat attttctcgg ctgtatcgat
    32521 gagtccggct gaaaatcctg attttatctt gtatgtgacg gtccaacaac ctgaacatta
    32581 ttcaggtatt cagttgggag aatttgccaa tcctatcttg gagcgggctt cagctatgaa
    32641 agactctctc aatcttcaaa caacagctaa agctttggag caagtaagtc aacaaagtcc
    32701 ttatcctatg cctagtgtca aggatatttc acctggtgat ttagcagaag aattgcgtcg
    32761 caatcttgta caacccatcg ttgtgggaac aggaacgaag attaaaaaca gttctgctga
    32821 agaagggaag aatcttgccc cgaaccagca agtccttatc ttatctgata aagcagagga
    32881 agttccagat atgtatggtt ggacaaagga gactgctgag acctttgcta agtggctcaa
    32941 tatagaactt gaatttcaag gttcgggctc tactgtgcag aagcaagatg ttcgtgctaa
    33001 cacagctatc aaggacatta aaaaaattac attaacttta ggagactaat atgtttattt
    33061 ccatcagtgc tggaattgtg acatttttac taactttagt aggaattccg gcctttatcc
    33121 aattttatag aaaggcgcaa attacaggcc agcagatgca tgaagatgtc aaacagcatc
    33181 aggcaaaagc tgggactcct acaatgggag gtttggtttt cttgattact tctgttttgg
    33241 ttgctttctt tttcgcccta tttagtagcc aattcagcaa taatgtggga atgattttgt
    33301 tcatattagt cttgtatggc ttggtcggat ttttagatga ctttctcaag gtctttcgta
    33361 aaatcaatga ggggcttaat cctaagcaaa aattagctct tcagcttcta ggtggaatta
    33421 tcttctatct tttctatgag cgtgggggcg atatcctgtc tgtctttggt tatccagttc
    33481 atttgggatt tttctatatt ttcttcgctc ttttctggct agtcggtttt tcaaacgcag
    33541 taaacttgac agacggtgtt gacggtttag ctagtatttc cgttgtgatt agtttgtctg
    33601 cctatggagt tattgcctat gtgcaaggtc agatggatat tcttctagtg attcttgcca
    33661 tgattggtgg tttgctcggt ttcttcatct ttaaccataa gcctgccaag gtctttatgg
    33721 gtgatgtggg aagtttggcc ctaggtggga tgctggcagc tatctctatg gctctccacc
    33781 aagaatggac tctcttgatt atcggaattg tatatgtttt tgaaacaact tctgttatga
    33841 tgcaagtcag ttatttcaaa ctgacaggtg gtaaacgtat tttccgtatg acgcctgtac
    33901 atcaccattt tgagcttggg ggattgtctg gtaaaggaaa tccttggagc gagtggaagg
    33961 ttgacttctt cttttgggga gtgggacttc tagcaagtct cctgacccta gcaattttgt
    34021 atttaatgta agaatggcat cctgatgttt cagggtgttt ttgtatttaa atacacaatg
    34081 aaaatcaaag aacaaactag aaagctaact ttaggctgct caaa
//
LOCUS       DAVBNO010000018        33677 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVBNO010000018 DAVBNO010000000
VERSION     DAVBNO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33677
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.018"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            184..414
                     /gene="blpU"
                     /locus_tag="VQ846_000975"
     CDS             184..414
                     /gene="blpU"
                     /locus_tag="VQ846_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET3075564.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            1129..3282
                     /locus_tag="VQ846_000976"
     CDS             1129..3282
                     /locus_tag="VQ846_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET3075565.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGEMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            3295..4644
                     /locus_tag="VQ846_000977"
     CDS             3295..4644
                     /locus_tag="VQ846_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET3075566.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSFYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4814..5521
                     /locus_tag="VQ846_000978"
     CDS             4814..5521
                     /locus_tag="VQ846_000978"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET3075567.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5723..9448
                     /locus_tag="VQ846_000979"
     CDS             5723..9448
                     /locus_tag="VQ846_000979"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET3075568.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDVTELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMNWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNSYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9541..10983
                     /locus_tag="VQ846_000980"
     CDS             9541..10983
                     /locus_tag="VQ846_000980"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET3075569.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPSNLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            11020..12042
                     /locus_tag="VQ846_000981"
     CDS             11020..12042
                     /locus_tag="VQ846_000981"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET3075570.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTESAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            12039..12584
                     /gene="purN"
                     /locus_tag="VQ846_000982"
     CDS             12039..12584
                     /gene="purN"
                     /locus_tag="VQ846_000982"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET3075571.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYIKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYLEVVKALFTD"
     gene            12668..13177
                     /locus_tag="VQ846_000983"
     CDS             12668..13177
                     /locus_tag="VQ846_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET3075572.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            13202..14749
                     /gene="purH"
                     /locus_tag="VQ846_000984"
     CDS             13202..14749
                     /gene="purH"
                     /locus_tag="VQ846_000984"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET3075573.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAKYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWEYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14871..16133
                     /gene="purD"
                     /locus_tag="VQ846_000985"
     CDS             14871..16133
                     /gene="purD"
                     /locus_tag="VQ846_000985"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET3075574.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLDYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            16536..17024
                     /gene="purE"
                     /locus_tag="VQ846_000986"
     CDS             16536..17024
                     /gene="purE"
                     /locus_tag="VQ846_000986"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET3075575.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTILPVIGVPVNSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDQSIATALADFAEEQGKIAEESSNE
                     LI"
     gene            17011..18102
                     /gene="purK"
                     /locus_tag="VQ846_000987"
     CDS             17011..18102
                     /gene="purK"
                     /locus_tag="VQ846_000987"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET3075576.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDVVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKIATGGYDGHGQNVIRSADD
                     LEEAYALADSAACVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPAIKLHAPAVMLNVLGQHVEAAEKYVTENPSTHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVEEFGEGIDF"
     gene            18112..18339
                     /locus_tag="VQ846_000988"
     CDS             18112..18339
                     /locus_tag="VQ846_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET3075577.1"
                     /translation="MIQIIVNAFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            18402..19700
                     /gene="purB"
                     /locus_tag="VQ846_000989"
     CDS             18402..19700
                     /gene="purB"
                     /locus_tag="VQ846_000989"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET3075578.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19755..23690)
                     /gene="strH"
                     /locus_tag="VQ846_000990"
     CDS             complement(19755..23690)
                     /gene="strH"
                     /locus_tag="VQ846_000990"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET3075579.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTTTTENQ
                     PTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEVS
                     STPSDKAEVVTQTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDEA
                     KAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTDL
                     HLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTDL
                     INYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAVA
                     FTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYEK
                     FIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVASS
                     KLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIPI
                     IGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRYT
                     AESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEALKEEEAKRE
                     VEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTIT
                     ANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSPG
                     HMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKIF
                     NFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYED
                     KDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGGF
                     LKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFAD
                     HNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTLV
                     ANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAGQ
                     ENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESGL
                     APTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADGK
                     EVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPAE
                     ESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(24006..24722)
                     /locus_tag="VQ846_000991"
     CDS             complement(24006..24722)
                     /locus_tag="VQ846_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET3075580.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            25070..26857
                     /locus_tag="VQ846_000992"
     CDS             25070..26857
                     /locus_tag="VQ846_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET3075581.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26854..27330
                     /locus_tag="VQ846_000993"
     CDS             26854..27330
                     /locus_tag="VQ846_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET3075582.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            27358..28263
                     /locus_tag="VQ846_000994"
     CDS             27358..28263
                     /locus_tag="VQ846_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET3075583.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            28250..29065
                     /locus_tag="VQ846_000995"
     CDS             28250..29065
                     /locus_tag="VQ846_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET3075584.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            29072..29476
                     /locus_tag="VQ846_000996"
     CDS             29072..29476
                     /locus_tag="VQ846_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET3075585.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            29775..30941
                     /locus_tag="VQ846_000997"
     CDS             29775..30941
                     /locus_tag="VQ846_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET3075586.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            31057..32094
                     /locus_tag="VQ846_000998"
     CDS             31057..32094
                     /locus_tag="VQ846_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET3075587.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            32301..33155
                     /locus_tag="VQ846_000999"
     CDS             32301..33155
                     /locus_tag="VQ846_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET3075588.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTCIRLAR"
     gene            33305..33545
                     /locus_tag="VQ846_001000"
                     /pseudo
     CDS             33305..33545
                     /locus_tag="VQ846_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
BASE COUNT         9356 a         6516 c         7912 g         9893 t
ORIGIN      
        1 gtaaaaaagt gattagaaaa catctttttt aaaaatagag atgattttga aacaaaaaag
       61 ctaattcaag acgtttctat gccaattcaa gatttggatg aaaaaaatta atagatactg
      121 ttatactaaa cttgtcaagt ttgtaacaag acaaatatta aaaataaaaa agaggtattc
      181 gttatgaata caaaaaagat gtcacaattt gaaattatgg atactgagat gcttgcttgc
      241 gttgaaggtg gcggatgcaa ttggggagat tttgccaaag caggtgttgg aggaggagca
      301 gtacgaggtc ttcagctagg aattaaaaca agaacatggc aaggtgcagc aactggtgct
      361 gcgggaggag ctatacttgg aggtgtggcc tatgcagcga catgttggtg gtaattatgg
      421 attttaaaag ttttattatt ggtttagtag ttggtatatt tggtccttat atggatgatt
      481 taattagaaa aaaattttta aagtcttcgg agaagaaaac agaaaaatct gttaaaaaat
      541 aatcaaaact ataaatgatg aatctgaatc aaaattattt tgcgcatgta aataggagtc
      601 ttatagtaac gagtcaaaaa aggagtaact atgaatcgta atttagaacg gtgttatcta
      661 ttctgactag gaatagatca taccagaggt agcttagaaa tagcagagac attagaaatt
      721 gaagtaataa ataggatgtc gtaagtgtta ctatcaatga tttatttgtt tcaagcttgt
      781 ctagggtgac agtaaaaaat caatttcctt tcaatagcat atttttagtg ggcagtactc
      841 ttgttctgcc tattttttta tccaaaaagt gcagttggga gggagatagg ctcatttggg
      901 aaggaagtcc agtttttgtt tagtgattgg ggtaagatag ttgttatcag atgagtttat
      961 actcttcgaa aatcaaattc aaaccacgtc aacgtcgcct tgccgtatat atgtgactga
     1021 cttcgtcagt cctatctaca acctcaaaac agtgttttga gcagcctgcg gctagtttcc
     1081 tagtttgctc tttgattttc attgagtatt agggaaaagg agatgaatat gaaatttggg
     1141 aaacgtcact atcgtccgca agtggatcag atggactgcg gtgtagcttc attagccatg
     1201 gtttttggct actatggtag ttattatttt ttggctcact tgcgagaatt ggctaagacg
     1261 accatggatg ggacgacggc tttgggcttg gtcaaggtgg cagaggagat tggttttgag
     1321 acgcgagcca ttaaggcgga tatgacgctt tttgacttgc cggatttgac ttttcctttt
     1381 gttgcccatg tgcttaagga agggaaattg ctccactact atgtggtgac tgggcaggat
     1441 aaggatagca ttcatattgc cgatccagat cccggggtga agttgactaa actgccacgt
     1501 gagcgttttg aggaagaatg gacaggagtg actcttttta tggcacctag tccagactat
     1561 aagccttata aggaacaaaa aaatggtctg ctctctttta tccctatatt agtgaagcag
     1621 cgtggcttga ttgccaatat cgttttggca acactcttgg taaccgggat taacattgtg
     1681 ggttcttatt atctgcagtc tatcattgat acctatgtgc cagatcagat gcgttcgaca
     1741 ctagggatta tttctattgg gctagtcatc gtctacatcc tccagcaaat cttgtcttac
     1801 gctcaggagt atctcttgct tgttttgggg caacgcttgt cgattgacgt gattttgtcc
     1861 tatatcaagc atgtttttca cctccctatg tccttctttg cgacacgcag gacaggggag
     1921 atcgtgtctc gttttacaga tgctaacagt atcatcgatg cgctggcttc gaccatcctt
     1981 tcgattttcc tagatgtgtc aacggttgtc attatttccc ttgttctatt ttcacaaaat
     2041 accaatctct ttttcatgac tttattggcg cttcctatct acacagtgat tatctttgcc
     2101 tttatgaagc cgtttgaaaa gatgaatcgg gataccatgg aagccaatgc ggttctgtct
     2161 tcttctatca ttgaggacat caacggtatt gagactatca agtccttgac cagtgaaagt
     2221 cagcgttacc aaaaaattga caaggaattt gtggattatc tgaagaaatc ctttacctat
     2281 agtcgagcag agagtcagca aaaggctctg aaaaaggttg cccatctctt gcttaatgtc
     2341 ggcattctct ggatgggggc tgttctggtc atggatggca agatgagttt ggggcagttg
     2401 attacctata ataccttgct ggtttacttt accaatcctt tggaaaatat catcaatctg
     2461 caaaccaagc ttcagacagc gcaggttgcc aataaccgtc taaatgaagt gtatctagta
     2521 gcttctgagt ttgaggagaa gaaaacagtt gaggatttga gcttgatgaa gggagagatg
     2581 accttcaagc aggttcatta caagtatggc tatggtcgag acgtcttgtc ggatatcaat
     2641 ttaaccgttc cccaagggtc taaggtggct tttgtgggga tttcagggtc aggtaagacg
     2701 actttggcca agatgatggt taatttttac gacccaagtc aaggggagat tagtctgggt
     2761 ggtgtcaatc tcaatcagat tgataaaaaa gccctgcgcc agtacatcaa ctatctgcct
     2821 caacagccct atgtctttaa cggaacgatt ttggagaatc ttcttttggg agccaaggag
     2881 gggacgacac aggaagatat cttacgggcg gtcgaattgg cagagattcg agaggatatc
     2941 gagcgcatgc cactgaatta ccagacagaa ttgacttcgg atggggcagg gatttcaggt
     3001 ggtcaacgtc agagaatcgc tttggcgcgt gctctcttga cagatgcgcc ggtcttgatt
     3061 ttggatgagg cgactagcag tttggatatt ttgacagaga agcggattgt cgataatctc
     3121 atggctttgg acaagacctt gattttcatt gctcaccgct tgactattgc tgagcggaca
     3181 gagaaggtag ttgtcttgga tcagggcaag attgtcgaag aaggaaagca tgctgatttg
     3241 cttgcacagg gtggctttta cgcccatttg gtcaatagct agaaagagga gaggatgaaa
     3301 ccagaatttt tagaaagtgc ggagttttat aatcgtcgtt accataattt ttccagtagt
     3361 gtgattgtac ccatggccct tctgctcgtg tttttacttg gctttgcaac tgttgcagag
     3421 aaggagatga gtttgtccac tagagctact gtcgaaccta gtcgtattct tgcaaatatc
     3481 cagtcaacta gcaacaatcg tattcttgtc aatcatttgg aagaaaataa gctggttaag
     3541 aagggggatc ttttggttca ataccaagaa ggggcagagg gtgtccaagc ggagtcctat
     3601 gccagtcagt tggacatgct aaaggatcaa aaaaagcaat tggagtatct gcaaaagagc
     3661 ctgcaagaag gggagaacca ctttccagag gaggataagt ttggctacca agccaccttt
     3721 cgcgactaca tcagtcaagc aggcagtctt agggctagta catcgcaaca aaatgagacc
     3781 atcgcgtccc agaatgcagc agctagccaa acccaagccg aaatcggcaa cctcatcagt
     3841 caaacagagg ctaaaattcg cgattaccag acagctaagt cagctattga aacaggtgct
     3901 tccttggccg gtcagaatct agcctactct ttttaccagt cctacaagtc tcagggcgag
     3961 gaaaatcccc aaactaaggt tcaggcagtt gcacaggttg aagcacagat ttctcagtta
     4021 gaatctagtc ttgctactta ccgtgtccag tatgcaggtt caggtaccca gcaagcctat
     4081 gcgtcagggt taagcagtca attggaatcc cttaaatccc aacacttggc aaaggttggt
     4141 caggaattga cccttctagc ccagaaaatc ttggaggcag agtcaggtaa gaaggtacag
     4201 ggaaatcttt tagacaaggg gaaaattacg gcgagtgagg atggggtgct tcatcttaat
     4261 cctgagacca gtgattctag catggttgca gaaggtgccc tactagccca actttatcca
     4321 tctttggaaa gagaagggaa agccaaactc acagcctatc taagttcaaa agatgtagca
     4381 agaatcaagg tcggtgattc tgttcgctat actacgactc atgatgccgg gaatcaactt
     4441 ttcctagatt ctactattac aagtattgat gcgacagcta ctaagactga gaaagggaat
     4501 ttctttaaaa tcgaggcgga gactaatcta acttcggagc aggctgaaaa acttaggtac
     4561 ggggtggaag gccgcttgca gatgattacg ggcaagaaaa gttatctacg ttattatttg
     4621 gatcaatttt tgaacaaaga gtaatgttcg tgtttttaga gttaaataat ttttaaactg
     4681 tgagaaagat tcttcttgca gttttttctt tacaattttt gaaaaatatc tactatttat
     4741 tcggttaaat tcttgtgttt tttggttttt tgtggtaaaa tgtgctcaag taatacgaaa
     4801 ggcgaacttt aaaatgtcaa aacaattgat ctattcggga aaagctaaag atatctatac
     4861 aactgaggat gaaaatctta ttatttcaac ttacaaggac caggcgactg ctttcaatgg
     4921 tgtcaagaag gagcagattg caggtaaggg agtcttgaat aatcagatct catcttttat
     4981 ttttgagaaa ttaaatgcgg ctggtgtggc gactcacttt gtggagaaac tttcagacac
     5041 ggaacaactc aataaaaagg ttaagattat tcctttggaa gtcgtgctcc gcaactatac
     5101 tgctggttcc ttttcaaaac gttttggtgt ggatgaagga atcgccttgg agactccgat
     5161 tgtcgaattt tactacaaaa atgatgattt ggatgatcca tttatcaatg atgagcatgt
     5221 gaaattccta cagattgcgg atgaccagca gattgcctac ttgaaggaag aaacgcgtcg
     5281 tatcaatgaa ctattgaaag tctggtttgc tgagattggg cttaaattga ttgactttaa
     5341 gctagagttc ggttttgaca aggatggcaa gattatcttg gcagacgaat tttcaccaga
     5401 taactgccgc ttgtgggatg ctgatggcaa ccacatggat aaggatgttt tccgtagagg
     5461 attgggagaa ctaaccgacg tttatgagat tgtttgggaa aagttgcagg aattgaaata
     5521 atctgtttgc aatggaaaac cttcgtctct caactaaaag gactcaggct gaaaaggtcc
     5581 cccagacctt ttcactctgt agagaactag gtgaactaac cgatgtttac gaaattgtct
     5641 aggaaaagtt gcagggttta aaataacaac ctcaaggctg tttgggaata ttgcaagagc
     5701 tgaaataaag gaataagaat tgatggataa acgtattttt gttgaaaaaa aggctgattt
     5761 tcaggtcaag tcagagagtt tggttagaga gctccagcac aacttgggac tgtcaagctt
     5821 gaaaagtatt cgtattgtgc aagtatatga tgtatttgac ttggctgagg acttgtttgc
     5881 acctgcagag aagcacattt tctctgagca ggtaaccgac catgttttag atgaagtatc
     5941 tgtgcaggcg gatcttgcta actatgcttt ctttgccatt gaaagtctac cagggcagtt
     6001 tgaccagcgt gcagcttcgt cacaggaagc cttgcttttg ttgggaagtt cgagtgacgt
     6061 gacagtcaac acagcccaac tttacttggt gaataaagat attgatgtga ctgagttgga
     6121 agctgtcaaa aactacctgc tcaatccagt tgattctcgt ttcaaggata tcacgacagg
     6181 gattgccaag caggagtttt cagagtcaga caagaccatt ccaaaattga ctttctttga
     6241 aagctataca gcagaagact ttgctcgcta caaggccgaa caagggatgg ccatggaagt
     6301 ggatgatttg ctctttatcc aagactactt taagtcaatc gggcgcgtgc caactgagac
     6361 tgaactcaag gttttggaca cttactggtc tgaccactgc cgtcatacga cttttgagac
     6421 agagttgaaa cacatcgact tttcagcttc taaatttcaa aagcaattgc agtcaaccta
     6481 tgacaagtat attgccatgc gcgaggaatt aggtcggtct gaaaaaccac aaaccttgat
     6541 ggatatggcg actattttcg gtcgttatga gcgtgctaat ggacgattgg atgatatgga
     6601 agtctctgac gaaatcaatg cctgctcagt tgaaattgaa gtggacgttg atggtgtcaa
     6661 ggaaccttgg ctcctcatgt ttaaaaatga aacccacaac catccaacag aaattgagcc
     6721 atttggtgga gcggctacct gtatcggtgg agctattcgt gatccgttgt caggccgttc
     6781 ctatgtttac caagccatgc gtatttcagg tgctggtgat attacagcac cgatttcgga
     6841 aactcgtgct gggaaattgc cacaacaagt catttctaaa acagcagctc atggttattc
     6901 ttcatatggt aaccagattg ggcttgccac aacctacgtt cgtgaatact tccacccagg
     6961 ctttgtagct aaacgtatgg aacttggtgc cgttgttggt gcggctccca agggcaatgt
     7021 tgtccgtgaa aaacctgaag caggtgatgt gatcatcctt ctcggaggca aaacaggtcg
     7081 tgatggtgtc ggtggtgcga cgggctcttc taaggttcaa acagttgagt ctgtagagac
     7141 tgctggtgct gaggttcaaa aaggaaatgc catcgaagaa cgcaagattc agcgcctctt
     7201 ccgtaatggc aatgtcactc gtctgatcaa gaagtccaat gactttgggg caggcggcgt
     7261 ctgtgtggct atcggtgaat tggcagacgg tcttgaaatc gacctcaaca aggtgcctct
     7321 taaataccag ggcttgaatg gtacagaaat tgccatctct gaatcacaag aacggatggc
     7381 ggtcgtggtt cgtcctgaag atgtggatgc cttcgttgcc gaatgtaaca aagaaaatat
     7441 tgatgctgtt gtggtggcga cagtaactga aaaaccaaat cttgtcatga actggaatgg
     7501 tgagacaatc gttgacttgg agcgtcgttt ccttgacacc aatggtgtgc gcgtggttgt
     7561 cgatgccaaa gttgtggaca aggatgtcaa actcccagaa gagcgtcaaa catctgctga
     7621 aacactggaa tcagataccc ttacggttct atctgacctc aaccatgcaa gtcaaaaagg
     7681 attacagact gtctttgact gctctgttgg acgctcaacg gttaatcacc cacttggtgg
     7741 tcgttaccaa ctcacaccaa ctgaggcatc tgtgcagaaa ttgccagttc aacacggtgt
     7801 gactcatact gcgtcggtca ttgctcaagg tttcaactca tatgtagctg aatggtctcc
     7861 ataccacggt gctgcttatg cggttatcga agcaactgct cgtttggtgg ctgctggtgc
     7921 caactggtct aaggctcgtt tctcttacca agaatatttc gagcggatgg acaagcaagc
     7981 agagcgtttt ggtcagccag tagccgccct tctaggctct attgaagctc agattcaact
     8041 tggcttgcca tctatcggtg gtaaggactc catgtctggt acctttgaag aattgaccgt
     8101 tccgccaacc ttggttgcct ttggggtgac gacggcagat agccgtaagg tgctctctcc
     8161 agaatttaaa gctgttgggg aaaatatcta ctacatccca ggtcaagccc tctctgcaga
     8221 gattgatttt gacttgatta agaaaaattt tgctcagttt gaagcaagcc aagctgacca
     8281 taaagtgaca tctgcatcag ctgtcaaata cggtggtgta gttgaaagtt tggctcttgc
     8341 tacctttgga aactatattg gtgcagaggt gaccttgcct gaacttaaaa cagctttgac
     8401 agctcaatta ggcggctttg tcttcacatc tcctgaagaa attgctggag tagagaagat
     8461 tggacaaacg aaagcagact ttacactgac tgtcaacggt gtgaagctag atggacacaa
     8521 gcttgacagt gcatttcaag ggacattgga agaagtttac ccaacagaat ttacccaagc
     8581 gaaagaacta gaagaagtac cagctgtggc atcagatgtt gtgattaaag ccaaagaaaa
     8641 ggttgaaaaa cctgtggttt acatcccagt ctttccagga accaactcag aatatgattc
     8701 agctaaggcc ttcgaaaaag aaggtgcaga ggtcaatttg gtgccattcg tgaccttgaa
     8761 tgaagaagct attgtcaagt cagttgaaac tatggttgac aatatcgaca agactaatat
     8821 tctcttcttt gccggtggat tctcggctgc ggatgaacca gatggttcag ctaagtttat
     8881 cgtcaatatc ctgcttaatg aaaaagtgcg tgtggctatt gatagcttta tcgcccgtgg
     8941 tggcttgatt atcggtattt gtaatggatt ccaagcctta gtcaaatcgg gtctcctacc
     9001 atacggaaac tttgaagatg ctaacagtac tagtccaacc ctcttctaca atgatgccaa
     9061 ccaacacgtg gccaagatgg tggaaactcg cattgccaat accaactcac catggttggc
     9121 tggtgtgcaa gtgggcgata tccacgctat tcctgtttcg cacggtgaag ggaagtttgt
     9181 cgtgacggct gaggaatttg cagagctccg tgacaatgga caaattttca gccaatacgt
     9241 tgactttaac ggtaaaccaa gtatggattc taagtacaat ccgaatggtt ctgtccatgc
     9301 catcgaagga attaccagca agaatggtca aatcatcggt aagatgggcc actcagaacg
     9361 ttatgaggat ggtcttttcc aaaatatccc aggcaataaa gaccaacacc tgttcgcatc
     9421 agcggttaaa catttcactg gaaaataaga cttacagatt ttctaataga tagtatcagt
     9481 aatgtaaaag tcatgtaaat ctagctcttg atgattacaa atgaaaatta ggtataaaaa
     9541 atgacatacg aagtaaaatc tcttaatgaa gaatgtggtg ttttcggtat ttggggacat
     9601 ccagatgctg ctaagttgac ctactttgga ctccacagtc ttcaacaccg tggtcaggag
     9661 ggggcaggaa tcctctccaa cgatcaagga caactgaagc gccatcgtga catggggctt
     9721 ttatcagaag ttttcagaaa tccatctaat ttggataaat tgacaggagc tggtgcgatt
     9781 gggcatgtgc gttatgcgac tgctggcgaa gcttctgttg ataacatcca acccttcctc
     9841 ttccgttttc acgatatgca gtttggtttg gctcataatg gaaatctgac caatgcagcc
     9901 tctctcaaga aagaactgga acaaagagga gcaattttca gcgcgacttc ggactctgaa
     9961 atcttggctc acctcattcg tcgcagtcat aatcctagcc tgatgggcaa aatcaaggaa
    10021 gcgctcagcc ttgtcaaagg tggttttgcc tatatcttgc tgtttgagga caagttgatt
    10081 gcggctcttg accctaatgg ctttcgtccg ctttctatcg ggaaaatggc caacggagcg
    10141 gtggttgttt cctctgaaac ctgtgctttt gaggtcattg gtgccgagtg gatttgtgat
    10201 ttgaagccag gtgagattgt gatcattgat gacgagggca ttcagtatga cagctataca
    10261 gatgataccc agttggcgat ttgttctatg gagtatatct actttgcccg ccctgattct
    10321 aatatccacg gtgtcaatgt ccatacggca cgtaagagaa tgggagcgca attggcgcga
    10381 gaatttaagc atgaggcaga tattgtagtt ggtgtgccca attcttccct aagcgcggct
    10441 atgggatttg cggaagaatc aggcttacca aatgaaatgg gtctgatcaa aaaccaatac
    10501 acccagcgaa cttttatcca accgactcaa gaattgcggg agcaaggagt gcggatgaaa
    10561 ctgtctgctg tttcgggtgt tgtcaaaggc aaacgtgtgg tcatggtgga tgattccatt
    10621 gtacgtggaa caacctctcg tcgtatcgtt cagctcttga aagaagcggg tgcgactgag
    10681 gttcacgttg ccattggaag tcctgcacta gcgtatccat gtttctacgg gattgatatc
    10741 cagacccgtc aggagctgat tgcagccaat catacggtcg aagaaactcg ccaaatcatt
    10801 ggtgcggaca gtctgactta tctttcaatt gatggcttga ttgagtcgat tggtatcgaa
    10861 acagatgcgc cgaacggtgg tctctgtgtc gcttactttg acggtgacta cccaacgcct
    10921 ctctatgact acgaagaaga ctatcgtaga agtttggaag aaaagaccag tttttacaag
    10981 taggcgacag attctccatt aaagaaaagg aaaaaataaa tgacaaataa aaatgcatat
    11041 gcccagtcgg gtgtggatgt tgaagcgggt tatgaagttg ttgaacggat taaaaagcac
    11101 gtggcccgta cggagagtgc aggtgtcatg ggagctcttg gtggctttgg tggtatgttt
    11161 gatctttcca agactggggt taaagaaccc gtcttgattt cagggactga cggtgtcgga
    11221 accaagctca tgttggctat caagtacgac aagcacgata ccatcgggca ggactgtgtg
    11281 gccatgtgtg tcaacgacat cattgctgca ggtgcggaac ccctctattt tctcgactac
    11341 gtagcgacag ggaagaatga accagctaag ctagaacaag tggttgctgg tgtggcagaa
    11401 ggttgtgtgc aggctggtgc tgccctcatc ggtggggaaa cggctgaaat gccgggcatg
    11461 tacggcgaag acgactatga cttggctggt tttgcggtcg gtgtggctga aaaatctcaa
    11521 atcattgacg gttcaaaggt ggtagaggga gatgttcttc tcggacttgc ttcaagtggg
    11581 attcactcaa atggttactc tttggttcgt cgtgtctttg cggattacac aggtgaggaa
    11641 gtcctaccag aattggaagg caagaaactt aaggaagttc tacttgagcc gactcgtatc
    11701 tatgtcaagg ctgtcttgcc gctcatcaaa gaagagttgg tcaacggcat tgcccacatc
    11761 acaggtggtg gctttatcga aaatgtccct cgtatgtttg cagatgacct agctgctgaa
    11821 attgatgaaa gcaaagttcc agtgcttcca attttcaaag cccttgaaaa atatggtcag
    11881 attaaacacg aagaaatgtt tgaaatcttc aatatgggtg tgggacttat gttggcggtc
    11941 agccctgaaa atgtagagcg tgtaaaagaa ttgttggatg aagcagtcta tgaaattggt
    12001 cgcatcgtca agaaagaaaa cgaaagtgtc attatcaaat gaaaaaaata gcggtttttg
    12061 cctctggtaa tggctcaaat tttcaggtga ttgccgaaga atttccagtg gagtttgtct
    12121 tttcagacca tcgtgatgcc tatgtgcttg agcgtgcaaa gcagctcggc gttctgtcct
    12181 atgcttttga actcaaggag tttgagagca aggcagacta cgaagcagcc cttgtcgaac
    12241 tcttggaaga acaccagatt gacttggttt gcctagcagg ctacataaaa atcgttggac
    12301 caaccttatt gtcggcttat gaaggtcgga ttgtcaatat tcatccagcc tacttgccag
    12361 aatttccagg agctcatggg attgaggatg cttggaatgc tggcgtgggt cagtctggtg
    12421 tgaccattca ctgggtggat tcgggtgtgg atacaggcca ggtcatcaaa caggttcgtg
    12481 tgccacgact agctgatgat accattgaca gatttgaagc tcgcatccat gaagcagagt
    12541 acaggttgta tctggaagta gtgaaggctc tatttacaga ttgacttttt gatgattcat
    12601 atgatatctt tgattttaaa ttggagtcag tgtttgttga agacggcttc aaacggaggt
    12661 atttgtaatg ttagaatcta aaaaaacaac tcgatatgta ttttatgtct atctgatgtt
    12721 attaacttgg ggaatcttat ttaagtttga aacaaatcct gaatttatag catttttctt
    12781 agctccaagg tatatcaatt ggattccatt ttcagaacca ctaatagtcg atggaaaaat
    12841 tgtttttgct gaaatgttat ttaatctgat ttcctttatt ccattaggtg tttgtttccc
    12901 tttgataaaa actaatttat ctagtttaag aatagtcggg acaggtttct tgattagttt
    12961 attgtttgag tgcttacagt atattttagc aataggtata acagatataa cggatttgac
    13021 tttaaatacg ctaggtgtct gtgtaggctt actgatttat caaattttta taagagtgtt
    13081 caaatcacag actagaaaat ggatcaatat cttaggtatg cttagccttg gttttgctta
    13141 tcttgtttta ctgttactgc atttaattgg tgtttaacta atgattaaaa aggagaatat
    13201 aatgactaaa cgcgtcttaa tcagcgtctc agacaaagcg ggcattgttg aatttgccca
    13261 agaactcaaa aaacttggtt gggagattat ctcaacaggt ggaactaagg ttgcccttga
    13321 taatgctggg gtggatacca ttgctatcga tgatgtgact ggtttcccag aaatgatgga
    13381 cggtcgtgtg aagaccctcc acccaaatat ccacggaggg cttctcgctc gtcgtgactt
    13441 ggatagccac ttggaagcgg ctaaggacaa caagattgag ctcattgacc ttgtggtggt
    13501 caacctttac ccatttaagg aaactatcct taaaccagat gtgacttatg ctgatgcagt
    13561 tgaaaatatc gatattggtg ggccatctat gcttcgttca gcagcgaaaa atcatgccag
    13621 tgttacagtt gtggtagatc ctgctgacta cgctgtggtt ttggacgaat tggcagcaaa
    13681 cggcgaaacc tcttatgaaa ctcgccaacg tttagcagcc aaagtatttc gtcacacagc
    13741 ggcttatgac gccttgattg caaaatactt cacagctcaa gtgggtgaaa gcaagcctga
    13801 aaaactcact ttgacttatg acctcaagca accaatgcgt tacggtgaga atcctcaaca
    13861 agacgcggac ttttaccaga aagctttgcc tacagattac tccattgctt cagccaaaca
    13921 gctcaacggg aaagaattgt catttaataa tatccgtgat gcagatgctg ctatccgtat
    13981 catccgtgac ttcaaagata gtccaaccgt tgtggctctc aaacacatga atccatgtgg
    14041 aattggtcaa gctgatgaca tcgagactgc ttgggaatac gcttatgagt ctgacccagt
    14101 gtctatcttt ggtggaattg tcgtcctcaa ccgtgaggtg gatgctgcga cagctgagaa
    14161 gatgcacggc gttttcctcg aaatcatcat tgcaccaagc tatacggatg aagcgctagc
    14221 cattttgatc aataaaaaga aaaacttgcg tatccttgcc ttgccattta atgctcaaga
    14281 ggctagcgaa gtggaagcag aatacacagg tgtagtcggt ggacttctcg tgcaaaatca
    14341 agacgtggtc aaggaaagcc cagctgactg gcaagtggtg accaaacgtc agccaaccga
    14401 gacagaagcg actgctcttg agttcgcttg gaaggctatc aagtacgtca aatcaaatgg
    14461 cattatcgtg accaacgacc acatgacact tggtgttggt ccaggtcaaa ccaaccgtgt
    14521 ggcttctgtt cgccttgcca ttgaccaagc caaagatcgt ctggacgggg cggtccttgc
    14581 ttcagatgcc ttcttcccat ttgcggataa cgtggaagaa atcgccaaag caggaattaa
    14641 ggccatcatc cagcccggtg gctcagtccg tgaccaagaa tctatcgaag ccgctgacaa
    14701 gtatggcttg accatggtct tcacaggagt gagacatttt agacattaag aagataaaag
    14761 ggaagaaaac agtttctttc cttttttggc ttaaaatgct aactgaaaca agattaaaac
    14821 gaactttttt gatataatgt tggtaaataa ttcgcaaaag aggttgagga atgaaactgc
    14881 ttgttgtcgg ttctggtggt cgtgagcatg cgattgctaa aaagttactt gaatcaaaag
    14941 acgtggaaaa agtctttgta gctcctggga atgatgggat gactctggat ggtttggaat
    15001 tggtaaatat ctctatttcc gaacattcta aattgattga cttcgcaaag accaatgatg
    15061 ttgcttggac ctttatcggc ccagatgatg cccttgctgc tggtatcgtg gatgatttta
    15121 accaagctgg gcttaaggcc tttggtccga ctagggctgc agcggagctg gagtggtcca
    15181 aggattttgc caaggaaatc atggtcaaat atggtgtttc gacagcagcc tatggcacat
    15241 tttcagattt tgaggaagca aaggcctata tcgaaaagca tggtgcgcct atcgtagtca
    15301 aggcggatgg cttggcgctt gggaagggtg tcgtcgttgc tgagacggtt gagcaagcgg
    15361 tcgaagccgc tcatgagatg cttttggaca ataaatttgg tgactcaggt gcgcgcgtgg
    15421 ttattgagga attccttgaa ggagaggaat tttcactctt tgcctttgtc aatggtgata
    15481 agttctacat catgccaacg gctcaggacc acaaacgtgc ctacgatggc gacaaagggc
    15541 ctaacacggg tggtatgggt gcctatgcgc cagttccaca cttaccacag agtgtagttg
    15601 atacagcggt tgacaccatt gtcaagccag ttctagaagg gatgattaaa gaaggtcgcc
    15661 cttatctggg agttctttac gcagggctta tcctgacagc tgatggaccg aaagtcatcg
    15721 agttcaacgc tcggttcgga gatccagaaa ctcagattat cttgcctcgc ttgacctctg
    15781 actttgctca aaatatcaca gatatcctgg atagcaagga gccaaatatc acgtggacgg
    15841 acaagggtgt gactctgggt gtggttgtcg catccaaggg ctacccgcta gattattcaa
    15901 agggtgttga gttgccagtc aaaaccgatg gtgacatcat tacctactat gcaggggcta
    15961 agtttgcgga aaatagcaga gcgctgctct caaacggcgg acgagtttat atgctcgtta
    16021 ccacagcaga taccgtcaaa gaagcccaag ccagcatcta ccaagaacta tcccaacaaa
    16081 aaatagaagg actcttctac cgaacagata tcggaagcaa ggcaattaag taaagatata
    16141 agaataacgc gacgcagtcg ccaaacacga taatggtcgt cgtggtgaaa agaccagaac
    16201 agtgaatgtt ctggtcaggg ggaaactttg agaccttagg ctcaaagttt aggaatgaaa
    16261 ccgaaggttt gcttccgcct ccatcaccta agaccattat caaaaagaaa aataaaaatt
    16321 cacaaaatac gttaatggtc gtatggtttg cgagcgttag cgagctaata tggagcaatt
    16381 accgccgttg tgaaagaacg attggatgat aatccaatcg ttcagggaaa ttggaagacc
    16441 ttgggtttcc aatttaggca tgagacacct ttggtggctg ctgccgtccc tcacaagcta
    16501 aggtgattgt tgaaaaagag gaaaaaggag aagaaatgaa accagtaatt tccatcatca
    16561 tgggctcaaa atccgactgg gcaaccatgc aaaaaacagc agaagtccta gaccgcttcg
    16621 gtgtagccta cgaaaagaaa gttgtttccg cacaccgtac accagacctc atgttcaaac
    16681 atgcagaaga agcccgtagc cgtggcatca agatcatcat cgcaggtgct ggtggcgcag
    16741 cccatttgcc aggtatggtc gctgctaaaa caatccttcc agtcattggt gtgccagtca
    16801 actctcgtgc tcttagtgga gtggattcac tctattctat cgttcagatg ccgggtgggg
    16861 tgcctgttgc gaccatggct atcggtgaag caggtgcgac aaatgcggcc ctcttcgctc
    16921 tccgtctcct ctcagtagag gatcagtcta tcgcgacagc attggcagat ttcgcagaag
    16981 aacaaggaaa aatcgcagag gagtcgtcaa atgagctcat ctaaaacaat cggaattatc
    17041 ggtggcggtc aactgggtca gatgatggcc atttctgcta tctacatggg gcacaaggtt
    17101 atcgcgctgg atcctgcggc ggattgcccg gcctctcgcg tggcggaaat cattgtggca
    17161 ccttataacg atgtggacgc cctccgtcag ttggcagacc gttgcgatgt cctcacttac
    17221 gagtttgaaa atgtcgacgc tgacggtttg gatgtcgtta tcaaggatgg acaactccct
    17281 caaggaacag atctgctccg catttcgcaa aatcgtattt ttgaaaagga ctttttgtca
    17341 aacaaggctc aagtcactgt ggcaccctac aaggtcgtga cttctagcct agacttggca
    17401 gatatcgact tgtcgaaaaa ctatgtcctt aagatcgcaa caggtggtta tgatggacac
    17461 gggcaaaatg tcattcgttc agcagacgat ttggaagaag cctatgcatt agccgactca
    17521 gcagcttgtg tcttggaaga atttgtcaat tttgaccttg agatttctgt catcgtgtca
    17581 ggaaatggga aggaggtgac gtttttccca gttcaggaaa atatccaccg caacaatatc
    17641 ctgtctaaga ccatcgtacc agcccgtatt tctgaaagtc tagcagacaa ggctaaagct
    17701 atggcagtgc gaatcgcaga acaactcaac ttgtctggaa ctctctgtgt ggaaatgttt
    17761 gcgacagctg atgacatcat tgtcaatgag attgctccac gaccacacaa ctcaggccac
    17821 tactctattg aagcctgtga cttttcacaa tttgacacgc atatattagg tgttctggga
    17881 gcaccattgc cagccatcaa actacatgcg cctgccgtca tgcttaacgt tctcggccag
    17941 catgttgagg ccgctgaaaa atatgtcaca gaaaatccaa gcacccacct ccacatgtat
    18001 ggtaaaatag aagcaaagca taatcgtaag atgggacatg tgactttgtt tagtgatgta
    18061 ccggatagtg tggaagagtt tggggaaggg attgattttt aggacaagtc tatgatacaa
    18121 attatcgtta atgcatttat tgaaaaggat aagactggag cagttgttga agtgttgtat
    18181 gctagtgctg accaagataa ggtacaagct aaatatgaag aactagctgc acaatacccc
    18241 gaaaattatt tagctatcta tgatgtaccg ctggatacgg atttgaatac actagatcat
    18301 tacccgtctg tgtttattgg aaaagaggag tttgagtaga aatcttggtt tacctagata
    18361 gcttattccc aacagcttaa gaagaaagga aagattaaca catgatcaac cgttactctc
    18421 gccctgagat ggcgaatatt tggagtgaag aaaataaata ccgtgcttgg cttgaggtgg
    18481 aaatcctctc tgacgaggca tgggctgagt tgggggaaat ccctaaggaa gatgtggctt
    18541 tgattcgcaa gaaggcggac tttgacatcg accgtatttt ggaaattgag caggagacgc
    18601 gccacgatgt ggtggctttc acgcgtgcgg tttctgagac tcttggtgaa gagcgcaagt
    18661 gggttcacta tgggttaact tctaccgacg tggtggatac tgcctatggt tacctctaca
    18721 agcaggccaa cgacatcatc cgtcgtgacc ttgaaaactt cactaatatc atcgctgaca
    18781 aggccaagga gcacaagttc accatcatga tggggcgtac tcatggtgtg cacgctgagc
    18841 cgacaacttt tggtcttaaa ttggcaactt ggtatagcga aatgaaacgc aatatcgagc
    18901 gcttcgagca tgcggctgct ggtgtagaag ctggtaagat ttctggtgcg gttgggaact
    18961 ttgccaatat cccaccattt gtagaggagt atgtctgcga taaacttggc atccgtgccc
    19021 aagaaatctc tacacaagtc cttcctcgtg accttcacgc tgagtacttt gcggttcttg
    19081 ccagcattgc gacttcaatc gaacgtatgg cgactgagat tcgtggtcta caaaaatctg
    19141 agcaacgcga agtagaagag ttctttgcta aagggcaaaa agggtcttca gcaatgcctc
    19201 acaaacgcaa cccaatcggt tctgaaaata tgactggtct ggcgcgtgtc attcgtggtc
    19261 acatgattac ggcttatgaa aacgtcgctc tctggcacga acgcgatatt tctcactcat
    19321 cagctgagcg tatcatcaca ccagatacga ccattttgat tgactacatg ctcaaccgtt
    19381 ttggaaatat cgtcaagaac ttgacagtct tcccagaaaa tatgatccga aacatgaact
    19441 cgacttttgg tcttatcttt agccaacggg ctatgttgac attgattgaa aaaggcatga
    19501 cccgtgagca agcctatgac ttggtgcaac caaaaacagc ctactcttgg gacaaccaag
    19561 tagactttaa accacttctt gaggcagatt cagaagtaac atcacgtctc actcaagaag
    19621 aaatcgatga aatcttcaac ccagtttatt acaccaaacg agtggatgat atctttgaac
    19681 gtcttggact aggtgattaa ttaaaaaata aacagcgagc ttcaatctcg ctgtttattt
    19741 tttatcgaaa agacttagtc ttcttttctt ttagtgagtc cataggctgc tagtgtggac
    19801 atgagtcctg cgactactag tcctgcagaa tcgtgagttc ctgtttcagg aagttttttc
    19861 tctgttacca caggagctgg atcttgagga agagctttgc tttcctcagc aggagcagtt
    19921 gatggagctg gttggcttgg gatttctagt tttggttttt cttcagcaat agcggcttgt
    19981 ccgttttcat cgcctacatg tgttaccata gttccgactt cgactatttg agtaacggct
    20041 tcctgtgcta cgacactatt tacaagtgtt ttcacttcct taccatcggc agaagtgctc
    20101 acagagtaga agttgctacg atgtccattg acgcccttag taatgacttg tgtttttcct
    20161 ttgagtaaga gtggattttc acgagtcact gtggtaaatg gaatttcttc tttttggata
    20221 tccagtctag gttttacctc agtagttggt gcaagaccac tttcatcacc cttgtgagtt
    20281 acaggagcgc caacttcaac cacttggttt ataacttctt tggttacctg gctatcaagg
    20341 actgtttctg ttgtttttcc attttcagtg agtacagaga tgtaatgagt tcgttcacct
    20401 ttgactcctg ctgtgataat attttcctga ccagctggga ggttaggatt ttctttcttg
    20461 ataacttcaa atggaatttc ttcagttctt gtgatgagtt ctggtctggt ttcaacattg
    20521 gcagccactt cattttcatc taggcttcct gaatgagttg cagctggttt gaggccttga
    20581 agagcggctt ttaggttggc tacaagtgtg tcaagctcag cttgtttatt acggttgagg
    20641 ttgtaattta gagctgtttt agctgcgtca agggcctcaa gactttcttt actatatcct
    20701 tctaagtttg taggaatttt agctaattct tcgcggagag cattataatt agcacggaag
    20761 tagtctttgt tgtggtctgc aaaggcagtc atgagttcaa agatttcctc ttccttgtac
    20821 tcagcgcttg gtctatctgc ccagattgaa agcatacttc cgactgttgg aagatctact
    20881 tcaggatatt tggtagaagc tagttgattg aatggtgttt ttccagtatt ctcaatagct
    20941 ttcttgagga aaccaccacc atcttctggt ttttgaccaa gaatgtagta ccagtcaccg
    21001 ttggtattca agaatttata gcctttgctt gctaggtatt gaggtgatgc gaggttatat
    21061 ccccaccagc ctttagacca gtaagaaatc aagacatctt tgtcaaactg aacatcgtcc
    21121 ttgtcttcat agtagaagcc atcgttgaag gccattggtt gaagccctct ttctttggcc
    21181 atagctgcga gggtgttggc atattcggca aatttgccat agagttgata ccacttgagg
    21241 tagtaccagc cttgggcact agtcgcatcg ttggcgtatt cgtcagtacc aaagttgaaa
    21301 atctttgttt tacctgcaaa gaagtccatg tatttaccga tgagggcttt tacaaagttc
    21361 atcgcttctt cgtttttcaa gtccatagtt gtttttgaaa ctttatcaaa gtgggcttga
    21421 ggatttttaa tacctaattt ttccatggca accagcatag catccatgtg acctggactg
    21481 ttaatagctg ggatgagacc gatgtcctta gatttagcgt attcaattag ctctgttact
    21541 tctgcctgtg ttagtgcagt accgtttgga tcgtcgtagt aagctttagt tccttcgata
    21601 atagcttttt taacgtcatc actagcatag gtttttccgt tggcagtaat ggtcatatca
    21661 tcgagtagaa agcgaagtcc gtcatttcct agaaggagat ggacatcaga atatccgagc
    21721 tcactggcct tgtctacgat gcgtttgagc tggttcagag taaagtattt gcgtccagca
    21781 tcgattgaga ttaccttgtt tttggcaagt ttttcaacct cacgtttagc ttcttcttct
    21841 tttagagctt caggcgtgag ggtcaagttg ttgacagttt cttgaagttt agcaatggct
    21901 tgatcaatcg tatcttgttg ggcacggcta aggttgctat cgagagagcg aatagctttt
    21961 tcagcttctt ttacggccgt gacgctttct gcagtataac ggttcaggtc ttttggtacc
    22021 tcgttaagtg cttgctctgc agattcataa tcagctgcga agtattcagc gttggcattt
    22081 gcaaaatgac gcatgagttt gaagaggcgt gatggtgaat aacgtgcaga tggagtgtca
    22141 gcccaagcag ctaccatacc accgatgatt gggatatcag ctccttctgt ttttggtaca
    22201 gaagtgattg gtgtgttttt aataccattg agcccctgat cgagattgta ccagccttgg
    22261 ccatcagcgt ttcgtccaag aacgtagtac caagcatcat tggtattaag gatttggtga
    22321 cctttttcag ctagtagttt agaagaagcg acatcgtagc ctccccaacc accagtccac
    22381 atagaaacga tgatgtcttt gtcaaaacta ccaaagcttg tgtcgctatt gtagtagata
    22441 ccgtcgttaa aagccattgg tttgagaccg tgcgatttta caatacgagc gaggtcattg
    22501 gcgtaggcaa taaatttttc atagcctttt acagggtagc cttcgtttgg atagtattta
    22561 tcagcttgaa gcacactcca acctttagca tctgtcgcat cattggcata ttcatcaagt
    22621 ccgatgttga agatttcagt ctttttcgcg aaataagcag catacttgtc gataagggct
    22681 tttgtaaaag cgacagcttg ttcgttgtca agatcgacag tacgggctga tttcttccca
    22741 aaatagctaa agttagggtt ttggattccc aattctttca tggcattgag aatcgcatcc
    22801 atgtgtccag gactatttac tgtcggaatg agaccgatac ctttatcttt ggcatagtta
    22861 atcagatctg tcatttgact ttctgttaag tgattgccgt ttggatcgtt gtaataatca
    22921 tttgtacctt tttcaatggc gcgtttgaca tcgtcactgg cataggtctt gccgttagct
    22981 gtgatgctca tatcgtccaa catgaaacgg agtccatcat ttccgactaa taggtgtaaa
    23041 tcagtgtagc cataatgttt cgctttatcg atgatttcct tgagttgttc tggtgagaaa
    23101 tatttacgtc cagcatcaat agaaacaatt ttctttttcg ctagtttttc atttacagtt
    23161 gcagcacgtt cctttcctgc ctctgtttcc ggtttgtcag cctctgcttt cgcttcatct
    23221 tttttagctg gtttatcctt gtcagtcttg tctgtatttg actctttaga atcaacctct
    23281 ttcgcttctt cctttttagg gctagcttct tctgcctttt tattagcagt ttctttttca
    23341 gcagaagttt gagttaccac ctctgcttta tcactaggag ttgaactaac ttcctcttgt
    23401 ggtttttctt ctgtttttgg aagactagct accttatcag tagctggagt ttctgtttct
    23461 acagtttttg gagcttctgg ttgaagctct gctttaggtg tttcctcagt ccgattttcg
    23521 gatgattgag gggaatcaga aactgtatgg atggtcggtt ggttttctgt agtagtagta
    23581 actccatcgg ctgcaacagt ctgtgcttgg aaggcaaatc caattagaac agaagctgct
    23641 cctacagcgt atttacgaat agaaaaacgc tgttgttttt catgtttcat tgcaaaacct
    23701 cctgattgca ttgttatatt gatagcgatt atataaatca acgcctttat tttatttctt
    23761 atattaacga gagtcaagag gagatgacaa aaaactataa taagtataaa aaaatataaa
    23821 atttaagctt aagatttcag attggtcgga aaaaatacgt atatatatct agtataattt
    23881 ttggttctat ttctataaaa tattccacaa attatagaat cttccaaaaa taggtaagcg
    23941 ctaccttttt ggtgtggtat aataagcata gaaaaagccc gagcgattag gctcaggctt
    24001 tcttcttagt gatcacggtc acatgagatg aatttaatct tgtagtaatc agatcgtttg
    24061 taagtttcac tgtattctaa aacttggcca gttgattcga gtttggtgat tttagtttgt
    24121 aggacagtag ggaattgttc atcgactccg aggactgaag ccgcatgttc tggagttgga
    24181 aagactattt cgttgatttc ttcaaagtgt tcatcattca tgtgaatgtg gtagtctaac
    24241 ttgaaacgat tatagataga actatagtat tcaaggtttg gataatttgc gttgatatat
    24301 tgttctggga tgtaggatgt atggtagata taaacgacac cgtttgattc gcggatacgt
    24361 tcaatcttgt agtagaattg atcgccgcgt agacccaatt tttccaggta aacaagcttg
    24421 tttccgcgtt caattgaaag aacagttacc ttatcatctt tagcattgaa gagttcaata
    24481 tctgaaaact ctacaagctt gtgtttgcgt gcacgtgaaa cgaaggttcc ttttccttgt
    24541 tggcggacaa tatagccatc tttggcaagg tcgtttaagg cgcgaacaac tgtgatagag
    24601 ctgacatcgt acattgaaat gagttctgct tcagtataaa atttatctcc actgctaaac
    24661 tgcccagaga tgattttatt ttttaattcg tcttttatgt attgatattt gggaattgcc
    24721 atatttttac ctctattttc cttctccata gaaaatttta ccataaaagc gaaaaaaata
    24781 gtagtctttt gaataaaaaa ttagaataat agtttgaaaa attaagctat atagggattg
    24841 atcatatttt ataaatgtat tctatacaaa attattttag gtcctatatt ccaaatttaa
    24901 agagcgtttg aaatgggtct aaaaatgttt gaaactaatt gaatgtgtgt ataatagtaa
    24961 aaatgcagta aatatcccga caatttctat tgacagttca aacagattgg tttataatta
    25021 atataacaac aaatgaaagc gcaaactttc gcggtcggaa ggtagtttta tgacacgatt
    25081 tgagatacga gatgatttct atctcgatgg aaaatcattt aagattttat ctggtgccat
    25141 tcattatttt agggttcctc cagaggattg gtatcattcg ctctataact tgaaggctct
    25201 tggttttaat acggtagaga cttatgttgc ttggaattta cacgagcctc gtgaaggtga
    25261 gtttcatttt gaaggtgatc tggatttaga gaaatttctc caaatagcgc aggatttggg
    25321 tctctacgca attgtgcgtc cgtctccatt tatctgtgcg gaatgggaat tcggtggctt
    25381 accagcttgg ctcttgacca agaacatgcg aattcgctca tctgacccag catatatcga
    25441 ggcagttggt cgctactatg atcagttatt gccaagactg gtgcctcgtt tgttggacaa
    25501 tggtggcaat attctcatga tgcaggttga aaatgagtat ggttcttacg gagaagataa
    25561 ggcttacctg agagcgattc gacagctaat ggaagagtgt ggcgtaacct gtcccctctt
    25621 tacatcagat ggtccatggc gagctactct gaaagctgga accttaattg aagaggacct
    25681 ctttgtaaca ggaaactttg gttctaaggc accttacaac ttttcgcaga tgcaggaatt
    25741 ctttgatgaa catggtaaga aatggccact catgtgtatg gagttctggg atggttggtt
    25801 caatcgctgg aaagaaccga ttatcacacg ggatcctaag gaattggcag atgcagttcg
    25861 agaggttttg gaacaaggct ctatcaatct ttacatgttc cacggtggta caaactttgg
    25921 tttcatgaat ggttgctcag ctcgaggaac tttggacctg ccacaagtta cgtcttatga
    25981 ttacgatgcc cttctggatg aagaaggaaa tccaactgct aaatatcttg cagtcaagaa
    26041 gatgatggca acacattttt cagagtatcc gcagttggaa ccactctaca aagagagtat
    26101 ggagttggat gctattccac tagttgaaaa agtttctttg tttgaaacct tagatagctt
    26161 gtcaagtcct gtagaaagtc tctatcctca aaagatggag gagctgggac aaagttatgg
    26221 ctacctactt tatcgaacag aaacaaactg ggatgcagaa gaagaaagac ttcgtatcat
    26281 tgatggtcga gatagggccc agctgtatgt cgatggtcag tgggttaaaa ctcaatatca
    26341 gacagagatt ggggaagata ttttttatca aggtaaaaag aaagggctat ctaggttaga
    26401 tatcttgata gaaaatatgg ggcgtgtcaa ctatgggcat aagttcttag cggatacgca
    26461 acgtaaggga attcggacag gggtctgtaa ggatctgcat ttcttactaa actggaaaca
    26521 ctatccactc ccactagaca atcctgagaa aattgatttt tcaaaaggat ggactcaagg
    26581 acaaccagcc ttttacgctt atgactttac agtcgaagag ccaaaagata cttacctaga
    26641 cttgtctgag tttggtaagg gggttgcctt tgtcaatggg cagaatctag gacgtttttg
    26701 gaacgttggc ccaactctct cactttatat ccctcatagc tatctcaagg aaggtgccaa
    26761 ccgcatcatt atctttgaaa cagaaggtca atataaagaa gagattcatt taactcgtaa
    26821 acctacacta aaacatataa agggggaaaa cttatgacaa ttgtaggatg ccgtattgat
    26881 ggacgtttga tccacggaca agtagccaat ctttgggctg gaaaactaaa tgtttcacgc
    26941 attatggttg tagacgacga agttgtcaac aacgatattg aaaagagtgg tttgaaactt
    27001 gcgacaccac caggtgtgaa attgagtatt ttgccagttg agaaagctgc agccaatatt
    27061 cttgctggca aatacgatag ccaacgtctc tttatcgtgg ctcgtaaacc agaccgcttc
    27121 cttggtttgg tagaagcagg tgtaccactt gaaaccctta atgttgggaa tatgtctcaa
    27181 acaccagaaa ctcgttctat tacacgttct atcaacgtag tagacaagga tgtggaagac
    27241 ttccacaaac tggcagaaaa aggtgttaaa cttactgctc agatggttcc aaatgatcca
    27301 atttcagact ttttgagctt attaaaatag gaaaaaaatt tttaggaggt cattgttatg
    27361 atacaatggt ggcaaatttt acttctcact ttgtactcag cttatcaaat ctgtgatgag
    27421 ttgacgatcg tttcatctgc aggttcccct gtatttgctg gtttcattac tggtttaatc
    27481 atgggagatg tgactactgg tttacttatc ggtggtaact tgcaactgtt cgttcttggg
    27541 gttggtacct tcggtggtgc ttctcgtatc gacgcaactt ctggtgcggt tcttgcgaca
    27601 gccttctctg tttcacaagg aattgatgca ccgcttgcca ttactacaat cgctgtacca
    27661 gtagcagctc tcttgactta cttcgacgtt cttggtcgta tgactactac cttcttcgct
    27721 caccgtgtgg atgctgcaat cgaacgcttt gactataaag gtattgaacg caactacttg
    27781 cttggtgcga ttccgtgggc tctatctcgt gcccttccag tcttctttgc ccttgctttt
    27841 ggtggtgcct ttgtacaatc agtagtagac ttcgttgaag cctacaaatg ggttgcagat
    27901 ggcttgacac ttgcaggacg tatgcttcca ggtcttggat ttgcaatctt gcttcgttac
    27961 cttccagtta aacgtaacct tcactacctt gctatgggat ttggtttgac agctatgttg
    28021 actgttcttt actcatatgt aacaggtctt ggtggcgctg ttgctggtat cgtaggtact
    28081 cttcctgctg aagttgctga aaaaattggt ttcgtgaaca acttcaaagg tttgtctatg
    28141 attggtattt ctatcgtagg tattttcctt gcagtgcttc acttcaaaaa tagccaaaaa
    28201 gtagctgtag cagcaccttc tacaccatca gaaagtgggg aaatcgaaga tgacgaattc
    28261 taattacaaa cttacaaaag aagattttaa tcaaatcaac aaacgtagct tgtttacttt
    28321 ccaattaggt tggaactacg aacgtatgca agcttctggt tacctttaca tgatcttgcc
    28381 tcagttgcgt aaaatgtatg gtgatggaac tcctgaattg aaagaaatga tgaaagttca
    28441 tactcaattc ttcaatactt caccattctt ccataccatt atcgctggtt ttgaccttgc
    28501 catggaagaa aaagatggtg taggttcaaa agacgccgtt aacggtatca agacaggttt
    28561 gatgggacca ttcgctcctc ttggggatac aatctttggt tcacttgtac ctgctatcat
    28621 ggggtcagtc gcagcaacta tggctatcgc tggccaacct tgggggatct tcctttggat
    28681 tgcagttgca gtagcgtatg acatcttccg ttggaaacag ttggaatttg cttacaaaga
    28741 aggggttaac cttatcaaca acatgcaaag taccttgaca gctttgattg acgctgcatc
    28801 tgtacttggt gtcttcatga tgggtgctct tgtagcaaca gtgattaact ttgaaatttc
    28861 ttacaagttg ccaatcggtg aaaagatgat tgatttccaa gacatcttga accaaatctt
    28921 cccacgtttg cttccagcaa tctttactgc ctttatcttc tggttgcttg gtaagaaagg
    28981 tatgaactct actaaagcta tcggtattat tatcgtactt gctttggctc tttctgccct
    29041 tggtcacttt gcacttggaa tgtaattcct tatgactaaa tcattaattt tggtgagcca
    29101 tggtcgcttc tgtgaggagc ttagaggtag cacagaaatg attatgggcc cacaagacaa
    29161 catttacaca gtagctcttc ttccagaaga tggcccagaa gaatttactg ctaaatttga
    29221 agctgttatt gaaggattgg atgatttcct agtctttgcg gatcttctcg gtgggacacc
    29281 ttgtaatgtg gtgagtcgct tgatcatgga aggtcgtgat attgaccttt acgcagggat
    29341 gaatcttcca atggtgattg aatttatcaa tgcgagcctt acaggcgcag atgcggacta
    29401 caagagccgt gctgcagaaa gcattgtgaa agttaatgac ctgttagcgg gcttcgatga
    29461 tgacgaagat gaataatact cttcgaaaat ctcttcaaac tacgtcaacg tcgccttgcc
    29521 gtaggtatat gttactgact tcgtcagtct tatccggcaa cctcaaaacg gtgttttgag
    29581 ctgacttcgt cagtcttatc cggcaacctc aaagcagtgc tttgagcagc ctgcggctag
    29641 tttcctacag attttagttg gaactcgatt caattcatgt gacaacgtga aaatcgttag
    29701 agcattttat atagaatata catgggaatg tagcttactc ccattcccat atttaataga
    29761 aaaagaggaa ctcaatgcta cattatacaa aagaagactt gctcgaattg ggtgcagaaa
    29821 tcactacgcg tgaaatctac caacagcctg atgtatggag agaagctttt gaattttatc
    29881 aagcaaaacg tgaagaaatt gcagccttcc tacaagaaat cgctgataaa catgactata
    29941 ttaaggttat cttgacaggt gctgggactt ctgcttatgt gggagatacc ttgctacctt
    30001 attttaagga agtctatgac gaacgcaaat ggaatttcaa tgctattgcg acaacagata
    30061 tcgttgccaa tccagcaacc tatttgaaaa aagatgtggc aactgtcctt gtgtcttttg
    30121 ctcgtagtgg gaactcgcct gaaagtgtgg cgactgttga tttggccaaa tccttggtgg
    30181 atgagcttta tcaagtgacg attacttgtg cagcagatgg taaattggct cttcaagctc
    30241 acggtgatga ccgtaatctc ttgctcttgc aaccagctgt ctctaatgat gctggatttg
    30301 ccatgacttc tagctttacg tctatgatgt tgacagctct cttggtcttt gatcctacag
    30361 aatttgctgt taagtctgaa cgttttgaag ttgtatctag tcttgcccgt aaagttttag
    30421 acaaggcaga agatgtcaaa gagcttgttg atttagactt taaccgtgtc atctatctag
    30481 gcgctggtcc tttctttgga cttgctcatg aagctcagct caagattttg gaattaactg
    30541 ctggtcaagt tgcgaccatg tatgaaagcc cagttggctt ccgtcacggt ccaaaatctc
    30601 ttatcaacga caatacagtt gttttggtct ttggtacaac gacagactac actcgtaagt
    30661 atgacttgga cttggttcgt gaagttgctg gtgaccagat tgctcgtcgt gttgtgcttt
    30721 tgagtgatca agcttttggt cttgaaaatg tcaaagaagt ggcccttggt tgtggcggtg
    30781 tcttgaatga tatttaccgt gtcttccctt acatcgttta tgcccaactc tttgccctat
    30841 tgacttcact caaggtagaa aataaaccag atacaccgtc tcctacaggt acagtaaacc
    30901 gtgtagtaca aggtgtcata attcacgaat atcaaaagta agacagtgtt tatgaattct
    30961 tgacaagagg atttgtaaat tatcagataa accatagatt gtcagtacgc tttctatggt
    31021 ttgtttgctt gagagaaata gtaaaaggag aacagaatga aagcatacac agagcgtgta
    31081 tttggaaatg ttgagggcga ggatgtcttg gcctatcgat ttgagacaga cggtggctac
    31141 caacttgagg ttatgactta tggcgcgacc atcttgcgct atgtcgcacc tgacaaggct
    31201 ggaaattttg ccaatgttat cttgggattt gatgactttg atagttatgt aggcaatagt
    31261 cccaagcatg gagcaagtgt aggtcctgta gcgggtcgta ttgcaggtgc gacctttgag
    31321 ctcaatggta agacctatga ccttgaggtt aataatgcta gcaactgtaa tcacagtggt
    31381 tcaactggtt gggattccag cttgtttgaa gttgaagaag taagcgatca tggcttgact
    31441 ctctacacag agcgtacaga tgggacagga gggttccctg gaaatctcaa gatttggatc
    31501 agttatcact tggaagaaac tggtgcctat gaaatcagct acaaggtaac gaccgatcag
    31561 gatacgctgg tcaatccaac caaccacagc tatttcaact tgtctggtga tttcacgcag
    31621 acgattgacc gtcatgtctt ccaactaaac acagagggca tttactcaat cgctcctgac
    31681 ggtgttcctg ccaaaactcc agaagccaac cgtgatgtgg tcaaacacgt ctacaatggt
    31741 accttgttga aggatatctt tgcagaagaa gatgagcaaa tccagctggc atcaggtttg
    31801 gatcatccat ttgcccttcc tgcaggccat gacaatgctg gattccttta tgaccaaaat
    31861 tcaggtcgct tcctgctttt caagacagaa gctccttgct ttgtggtcta cacagcaaac
    31921 tttgtggatg aaagtgtcat cataggaggt cagccaatgc tacagcacaa tgggattgct
    31981 cttgaagcgc aagctttacc agatgccatt cacagtgacc ttaaaggcca agtcattctt
    32041 aaggctggtc aaaccttcac cagtaagaca cgttatgaac ttgttgtgaa ataaaaaagc
    32101 tggtcaaaaa gtctttaaaa ttaaaataac gcatattgtc aggtgatgaa aagtttgata
    32161 ctatgcgttt tgttatgaaa tgatttactt tatcttatcc tgaaatggtg ttatttttga
    32221 gttatctggc ttgactttct tgatggaaat tatataatag ttgtaacaat aataattaca
    32281 acgagaaaag aggaccattt atgacctatg aatacaagag tcacatttat ttggcagaga
    32341 cagttttaaa tgtaaaggat ttggccagtc aaacagcctt ttatcagcaa gttattggtt
    32401 tagaaatctt atctcaaact gagacagagt ccattctagg ccttggtgga aaagtcttgg
    32461 tacagctgat tcaagcacaa gagagcggag aagtgaggga acattatggt ctttaccatt
    32521 tggccattct cttacccaca cgcaaggctt tggctgatgt cttgaaacac ctgacagatt
    32581 tacagattcc tcttgtcggc ggtgcagacc atggttacag tgaagctatt tacttggaag
    32641 atttggaggg aaatggcatt gaactctatc gagataagcc agtttccaca tgggatattc
    32701 gagaagatgg acgcattatc ggagtgacgg aggctcttgc ggcgcaggac atttatgagt
    32761 tgggggaaag agtagagccc tttatcctgg cagaaggtac gagaatgggg catattcatc
    32821 tttcagtcaa ggatagtcga aagtccagcc agttttatca aaaggtgtta gggctagagg
    32881 ataaattcag tgtgcctagc gctagttgga ttgcggctgg agattaccat catcatttag
    32941 cagttaacga atggggagga aaaggtctgg cttcgcgtaa gcaaggctta ccaggtttag
    33001 cctactatgt catcgaagtc gcacataaaa aagaactgtt aacgattgcc caacgagcac
    33061 aagaagttga cgcaccaatc aaatggatga catcgagcca attggaaatc acagactcag
    33121 atggcatcgt gacctgtatt cgtttagcaa ggtaaaatta ggaattttta ataagttata
    33181 agtattgcta gtttgctttt tgaacggtct ttggtaacag aactgcgtga gctggatggg
    33241 tgagaatttt gacaaagaag ctgattagag atataatgaa gaaaaatcga gcaaggaaaa
    33301 agaaatgaca aattcaaagt atattactcg tttgaaacgt tcagagggcc agttgcgtgg
    33361 gattcaaaag atgattgaag gagatcgtga cgcaactgac agcagtgaga tctagtgtag
    33421 agcgcgtgat tgagatgata attaccgaaa atcttactga atgtatcaat cagccactag
    33481 atgattctga agctcaaaag gaacgcctag aaaaggctat ccgatacttg attaaacgga
    33541 aataaatgaa tgttttgaga cgatatgtgc aaaggtctat taactattag aatagtttaa
    33601 tgtgagtcaa aagaagttgt aaaaaatctt taaaattaaa aaaacgcata gtatcaggtg
    33661 ttgaatgtac tgacccc
//
LOCUS       DAVBNO010000019        33585 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVBNO010000019 DAVBNO010000000
VERSION     DAVBNO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33585
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.019"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            364..624
                     /locus_tag="VQ846_001001"
     CDS             364..624
                     /locus_tag="VQ846_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075589.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            578..798
                     /locus_tag="VQ846_001002"
                     /pseudo
     CDS             578..798
                     /locus_tag="VQ846_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1117..2046
                     /locus_tag="VQ846_001003"
     CDS             1117..2046
                     /locus_tag="VQ846_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET3075590.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGTLWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            2060..2983
                     /locus_tag="VQ846_001004"
     CDS             2060..2983
                     /locus_tag="VQ846_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET3075591.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            3067..4542
                     /locus_tag="VQ846_001005"
     CDS             3067..4542
                     /locus_tag="VQ846_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET3075592.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            4814..5800
                     /locus_tag="VQ846_001006"
     CDS             4814..5800
                     /locus_tag="VQ846_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET3075593.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            5922..6782
                     /locus_tag="VQ846_001007"
     CDS             5922..6782
                     /locus_tag="VQ846_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET3075594.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPLSFYHQLDANQIEVQPILD
                     KDFKALAQEE"
     gene            complement(6959..8023)
                     /locus_tag="VQ846_001008"
     CDS             complement(6959..8023)
                     /locus_tag="VQ846_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075595.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVPQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(8086..8997)
                     /locus_tag="VQ846_001009"
     CDS             complement(8086..8997)
                     /locus_tag="VQ846_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET3075596.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPIY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILNQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            complement(8990..9583)
                     /locus_tag="VQ846_001010"
     CDS             complement(8990..9583)
                     /locus_tag="VQ846_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET3075597.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKRKRGNQHA"
     gene            complement(9570..9896)
                     /locus_tag="VQ846_001011"
     CDS             complement(9570..9896)
                     /locus_tag="VQ846_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET3075598.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            complement(10035..11201)
                     /locus_tag="VQ846_001012"
     CDS             complement(10035..11201)
                     /locus_tag="VQ846_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET3075599.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            11259..12398
                     /locus_tag="VQ846_001013"
     CDS             11259..12398
                     /locus_tag="VQ846_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET3075600.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKGKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDAEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGGLILKIDAHSKVTESFVMNNVAVIQQGEFVCGGPRP
                     TIVKGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLSLLTLLTLLKHK
                     NGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTVIYLEK"
     gene            12466..14316
                     /locus_tag="VQ846_001014"
     CDS             12466..14316
                     /locus_tag="VQ846_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET3075601.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMVDFWGLSASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSAFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(14495..14593)
                     /locus_tag="VQ846_001015"
                     /pseudo
     CDS             complement(14495..14593)
                     /locus_tag="VQ846_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(14619..15251)
                     /locus_tag="VQ846_001016"
     CDS             complement(14619..15251)
                     /locus_tag="VQ846_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET3075602.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(15273..16145)
                     /gene="sdaAA"
                     /locus_tag="VQ846_001017"
     CDS             complement(15273..16145)
                     /gene="sdaAA"
                     /locus_tag="VQ846_001017"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET3075603.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(16154..16825)
                     /gene="sdaAB"
                     /locus_tag="VQ846_001018"
     CDS             complement(16154..16825)
                     /gene="sdaAB"
                     /locus_tag="VQ846_001018"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET3075604.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            17067..17639
                     /locus_tag="VQ846_001019"
     CDS             17067..17639
                     /locus_tag="VQ846_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET3075605.1"
                     /translation="MKSITKKIKATLAGVAALFAVFSPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(17691..18011)
                     /locus_tag="VQ846_001020"
     CDS             complement(17691..18011)
                     /locus_tag="VQ846_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET3075606.1"
                     /translation="MQRLEVYKNYQHLYDLQIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            18436..18720
                     /locus_tag="VQ846_001021"
     CDS             18436..18720
                     /locus_tag="VQ846_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET3075607.1"
                     /translation="MRKLVKIGVASLMVCSILASTSVLAVWVDGGQWNYGVGWSGNFG
                     YSDYLHSTRSHTATVKDGNKFSKDRAEAEAWARASIFKFPPTGMEYFYGF"
     gene            18775..20883
                     /locus_tag="VQ846_001022"
     CDS             18775..20883
                     /locus_tag="VQ846_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET3075608.1"
                     /translation="MKKISHLCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVITFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     VLASRMEKRSLVELLKGGIL"
     gene            20880..21521
                     /locus_tag="VQ846_001023"
     CDS             20880..21521
                     /locus_tag="VQ846_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075609.1"
                     /translation="MIELENITKTIGGKVILDHLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLSKKDKLKKVEEILERVGLSSTLHQKVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            21728..22534
                     /locus_tag="VQ846_001024"
     CDS             21728..22534
                     /locus_tag="VQ846_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET3075610.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSIPYYTAKNKLIVKKSDLTTYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVSKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            22500..22970
                     /locus_tag="VQ846_001025"
     CDS             22500..22970
                     /locus_tag="VQ846_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET3075611.1"
                     /translation="MRMPLKHPLKNRKKEKNMSKEKVILAYSGGLDTSVAITWLKKDY
                     DVVSVCMDVGEGKDLDFIHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKY
                     PLVSALSRPLISKKLVEIAHQTGATTIAHGCTGKGNDQVEYQIAVAKKANEAKK"
     gene            complement(23260..23580)
                     /locus_tag="VQ846_001026"
     CDS             complement(23260..23580)
                     /locus_tag="VQ846_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET3075612.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            24344..24724
                     /locus_tag="VQ846_001027"
     CDS             24344..24724
                     /locus_tag="VQ846_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HET3075613.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGRNWWNSRGSFATVLDVGL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTG
                     FSLGGAIAYGADWADGSLDGYIFA"
     gene            25098..26156
                     /locus_tag="VQ846_001028"
     CDS             25098..26156
                     /locus_tag="VQ846_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075614.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIGTVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFMFSYILIFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALGDLSKQYLNRNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKR"
     gene            26471..27447
                     /locus_tag="VQ846_001029"
                     /pseudo
     CDS             26471..27447
                     /locus_tag="VQ846_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27824..28198
                     /locus_tag="VQ846_001030"
     CDS             27824..28198
                     /locus_tag="VQ846_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET3075615.1"
                     /translation="MELVLPNNYVALEQEEMMYFDGGFSIPRWPVATAINIAFNDVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGN
                     AITTIWANNDVNRRDPNLNALW"
     gene            28406..29308
                     /locus_tag="VQ846_001031"
     CDS             28406..29308
                     /locus_tag="VQ846_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075616.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSP
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESDLTKTLKDKIQKSELSYSDLMSLQLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            29364..29717
                     /locus_tag="VQ846_001032"
     CDS             29364..29717
                     /locus_tag="VQ846_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET3075617.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGEAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYYIGYGVLNRGCDIN
                     GNPYPWDGFISATVK"
     gene            29714..30442
                     /locus_tag="VQ846_001033"
     CDS             29714..30442
                     /locus_tag="VQ846_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075618.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFITELIIFHL
                     IGKDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNHVLTLI
                     FLIKICADTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEFELFKNSK"
     gene            31574..31813
                     /locus_tag="VQ846_001034"
     CDS             31574..31813
                     /locus_tag="VQ846_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075619.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTDLHHLF
                     DLHKITRDYAGQTKPAIQAGVFILEDFRNILCTMM"
     gene            32363..>33585
                     /locus_tag="VQ846_001035"
     CDS             32363..>33585
                     /locus_tag="VQ846_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HET3075620.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASQPTVVRAEEAPVASQSKAEK
                     DYDAAKRDAENAKKALEEAKRAQKKYEDDQKKTEEKAKKEKEASKEEQAANLKYQQEL
                     VKYASEKDSVKKAKILKEVEEAEKEHKKKRAEFEKVRSEVIPSAEELKKTRQKAEEAK
                     AKEAELIKKVEEAEKKVTEAKQKLDAERAKEVALQAKIAELENEVYRLETELKGIDES
                     DSEDYVKEGLRAPLQSELDAKRTKLSTLEELSDKIDELDAEIAKLEKNVEYFKKTDAE
                     QTEQYLAAAEKDLADKKAELEKTEADLKKAVNEPEKPAEETPAPAPKPEQPAPAPKPE
                     KTDDQQAEEDYARRSEEEYNRLPQQQPPKAEKPAPAPKPEQPVPAPKTGWKQENGMWY
                     FYNTDGSMATGWLQN"
BASE COUNT        10539 a         5576 c         6971 g        10499 t
ORIGIN      
        1 tttcctgata gagttgttca catcttattt caaatcacta taaaagctct ataatctctt
       61 tattaagatt tgcccatcag acgaaatata atggattgaa actagaatag tacacatcta
      121 cttctaaaac attgttagaa atcgatttga ctgtcctgat cgatttgtcc tgttcttgtt
      181 tcattttact atagaacgat ttgaaggcgt ttatgatatt tagctgtacg agagtctttt
      241 aaaagtgttt tgatagtttg gatttcttct ttagttgatt tcatatcact gttatataat
      301 gctttttgat tttagtctag tattagtaag cagactcaaa ccttagaaaa tcttttgaga
      361 tcattgacag gtgtatcgaa tcctaccaca gattcagcac gtctggtttt ggcagaggct
      421 aagaaagctt ttgcagatga tagtttgaca gagcaaggtt tacgtgatat tttgcaaaca
      481 gtcaaagatg ccattgcttc cctagagtcc attaaggaaa gccagtcagc aactaaggat
      541 ggaggacaaa cagcgggcaa agaaacagca ggagaagatg agtctgcaaa tcaaactcaa
      601 gaaattagcc aaggaattaa gtaaacttct taaagactca aaccttgaaa cagtagataa
      661 ggatgtcctt gaaaactctc aaaaagaact tcaaaaagca gttttgttct tggcagatga
      721 aaaagctctg agcatactga agcagaggtg attgataatc ttaaagaggt tattgctaag
      781 ttgaaagcaa acgcctaata acatattaaa aatctccaga ttaggaacta tctctgagtt
      841 ccctagtctg gagatttttc agtatacccc gttgttggat gcttataaca gagtgacgcc
      901 atcactgggc taatagagat aaaataaaca attgatttag gacatttgtt tgatagtggt
      961 gataaagttt taaatctgct acataatttg tggagttttt tctataatta ggagtttaac
     1021 ctaacctgca aataggagta tactaataat gtaatcgtta tcaaaagtct aaaaaagaat
     1081 ttttagatgg atatcaaaat aaaaagggag gaaattatga aaaagttttc aaaaacattg
     1141 agagacaact ggatctttct cttgatggtt ttgccaggga cactctggtt gattctattc
     1201 ttttacattc cagtatttgg gaacgtggtt gccttcaaag actaccacat gaccagtaat
     1261 ggtttcatag atagtatcat aaatagtaaa tgggtcggac tcgataattt cagattctta
     1321 tttagttcaa gagacgcctt tattatcaca cgaaatactg tcctctacaa tcttggcttt
     1381 atctttctag gtttagttgt atctgtaggg attgccatta tcctcagcga gctccgttct
     1441 aagagaatgg tgaagatttt ccaaacttct atgttgttcc cttacttctt gtcttgggtt
     1501 atcatcagtt tctttacaga tgccttccta aatattgata aaggggtgtt caatcatcta
     1561 ttggaaagtc ttggtctcaa agaagtcaat ttttacgctg acctgggcat ctggccctat
     1621 ctcctacttt tcctaggtat ttggaaaggc tttggatata gcagtgtcat gtactatgcg
     1681 acgatcatgg gaattgatcc aacctactac gaagcagcga cagtggacgg agctagcaag
     1741 tggcaacgta ttcgcaacgt aaccattcct cagttgactc cgcttgtaac tgtattgacc
     1801 atccttgcag tcggaaatat cttccgcgca gactttggtc tcttctatca aatcccacac
     1861 aatgctggtc agctttacaa tgtaaccaac gttttggacg tatatgtctt taatggtttg
     1921 actcagacag cagatatcgg tatggctgca gcagccggtc tttaccaatc cgttgttggt
     1981 ttgattctgg ttatcctatc aaacttgctt gcaagacgag tcgatccaaa ctcagctttg
     2041 ttctagaaag gaggagaata tggcaaaaaa gaaaattaaa aaagaaaaaa ttgataatgt
     2101 cggcattcac tccttcagta agaaagcaga tatcttcttc agtatcattt cgggtttgat
     2161 tgccctctct tgtatcctac cttttgtatt cgttatcatt atttcagtga cagacgagaa
     2221 aagcctcctc caatacggct atagcttctt cccatcccaa tttggcttag acggttttga
     2281 gtttttggca cagtttaagg ataaaatcct acaagccctc ttcatctctg tctttgtaac
     2341 cgtagttggg acattgacaa atgtctttat cacaacaacc tatgcctacg ctatttcacg
     2401 gacaaccttt aagtatcgca gattttttac catcttcgtc cttctcagta tgttgttcaa
     2461 cgctggtttg gtaccaggtt atatcatggt gacccgtgta cttcagcttg gtgacactgt
     2521 ttgggccttg attgttccaa tgcttctctc accattcaat atcatcttga tgcgttcctt
     2581 cttcaagaag accattccag aagccattct cgaatcggct cgtatcgatg gtgccagtga
     2641 ggcccggatt ttcttccaaa tctgtttgcc attgtcacta ccaggtatcg caaccatcac
     2701 gcttttgaca gctcttggtt tctggaatga ctggttcaac gcccttcttt acatcaagag
     2761 tgataacttg tatccattgc aatatttgct catgcaaatc caacaaaata tggactacat
     2821 tgccaaagcg gtcggcttga ctggtcaact gggagttgct ctaccgaaag aaacaggtcg
     2881 tatggccatg gttgtggtag caacccttcc aatcgcgatt ttgtatccat tcttccaacg
     2941 ctactttgta aaaggtttga ctatcggtgg tgtgaaagaa tagcacttgt ttccaaactt
     3001 cccttgcgtg aagttaagat cattacattg tttataagtt taaaaataaa aaaaggagtt
     3061 tttatcatga aaaactggaa aaaatatgct tttgcatctg ctagtgtagt tgctttggct
     3121 gcaggtcttg ctgcttgtgg caacttgaca ggtaacagca aaaaagctgc tgattcaggt
     3181 gacaaacctg ttatcaaaat gtaccaaatc ggtgacaaac cagataactt ggatgaattg
     3241 ttagcaaatg ccaacaaaat catcgaagaa aaagttggtg ctaaattgga tatccaatac
     3301 cttggctggg gtgactatgg taagaaaatg tcagttatca catcatctgg tgaaaactat
     3361 gatatcgcct ttgcagataa ctatattgta aatgctcaaa aaggtgctta tgctgacttg
     3421 acagaattgt acaaaaaaga aggtaaagac ctttacaagg cacttgatcc agcttatatc
     3481 aagggtaata ctgtaaatgg taagatttac gctgtaccag ttgcagccaa cgtagcatca
     3541 tctcaaaact ttgccttcaa cggaactctc cttgccaaat atggtatcga tatttcaggt
     3601 gttacttctt acgaaactct tgagccagtc ttgaaacaaa tcaaagaaaa agctccagac
     3661 gtagtaccat ttgctattgg taaagttttc atcccatctg ataactttga ctacccagta
     3721 gcaaacggtc ttccattcgt tattgacctt gaaggcgata ctactaaagt tgtaaaccgt
     3781 tacgaagtac ctcgtttcaa agaacacttg aagactcttc acaaattcta tgaagctggc
     3841 tacattccaa aagacgtcgc aacaagcgat acttcctttg accttcaaca agatacttgg
     3901 ttcgttcgtg aagaaacagt aggaccagct gactacggta acagcttgct ttcacgtgtt
     3961 gccaacaaag atatccaaat caaaccaatt actaacttca tcaagaaaaa ccaaacaaca
     4021 caagttgcta actttgtcat ctcaaacaac tctaagaaca aagaaaaatc aatggaaatc
     4081 ttgaacctct tgaatacgaa tccagaactc ttgaacggtc ttgtttacgg tccagaaggc
     4141 aagaactggg aaaaaattga aggtaaagaa aaccgtgttc gcgttcttga tggctacaaa
     4201 ggaaacactc acatgggtgg atggaacact ggtaacaact ggatccttta catcaacgaa
     4261 aacgttacag accaacaaat cgaaaattct aagaaagaat tggcagaagc taaagaatct
     4321 ccagcgcttg gatttatctt caatactgac aatgtgaaat ctgaaatctc agctattgct
     4381 aacacaatgc aacaatttga tacagctatc aacactggta ctgtagaccc agataaagcg
     4441 attccagaat tgatggaaaa attgaaatct gaaggtgcct acgaaaaagt attgaacgaa
     4501 atgcaaaaac aatacgatga attcttgaaa aacaaaaaat aatcagctaa attgatttcg
     4561 tgtattcatt cctaactcct aaaataagaa tcactgcttt cctcaggttg gtctggggga
     4621 gacagtggtt tttttggcac aaggatatag atatccatgt tttctcttgt ccttatttta
     4681 accattcagg aaatttcgag aaccctctac aatttccagt caaataaatt gcattcgttt
     4741 tctcaagcag gtatactagt gtagataaat aaaaaattta gaaaatttaa gaatagaaaa
     4801 gagaacaaat cttatggcaa aagatattcg tgtcttactt tactaccttt atactccaat
     4861 tgaaaatgca gagcaatttg ctgcagacca cttggctttc tgtaaatcaa tcggtcttaa
     4921 aggccgtatc ctagtcgctg acgagggaat taacggaaca gtttcaggtg actatgaaac
     4981 aactcaaaaa tacatggact acgttcacag ccttccagga atggaagaac tctggttcaa
     5041 gattgacgaa gaaagtgaac aagccttcaa gaagatgttt gttcgctaca agaaagaaat
     5101 tgtccacctt ggtttggaag acaacgactt tgacaatgac atcaacccac ttgaaacaac
     5161 aggtgcttac ttgtctccaa aagagttcaa agaagcgctt cttgataaag ataccgttgt
     5221 ccttgacaca cgtaatgatt atgagtatga cctaggacat ttccgtgggg ctattcgccc
     5281 agatattcgc aacttccgtg agttaccaca atgggtccgt gataacaagg aaaaattcat
     5341 ggacaagcgt gtcgtggttt actgtacagg tggcgttcgc tgtgagaaat tctcaggctg
     5401 gatggttcgt gaaggttaca aagatgtcgg ccaattgcac ggaggaatcg caacttacgg
     5461 taaagaccca gaagttcaag gtgagctttg ggatgggaaa atgtacgtct ttgacgagcg
     5521 tattgccgtt gatgtcaacc atgtcaaccc aactatcgta gggaaagact ggtttgatgg
     5581 aacaccatgt gaacgttatg tcaactgtgg aaatccattc tgtaaccgtc gtatcttgac
     5641 atcagaagaa aatgaagaca agtaccttcg tggatgctca cacgagtgcc gtgttcaccc
     5701 acgtaaccgc tatgtttcaa aaaatgaatt gacacaagct gaagtgattg agcgcctagc
     5761 cgctatcggt gaaagcttgg atcaagcagc tactgtataa gatcaaacag tcctcagggg
     5821 ctgtttttct atgctttgta ctcaaaaatc taaagtttgt ttctgtatct ttcagaaaaa
     5881 taaggtatac tgtatgtaaa cgatttcaaa ggagtccagt tatggcaaaa acatttttta
     5941 ttccaaataa acagagcatt ttaggagaac aagagatttt gaatgccaag tcgatcttgg
     6001 ccttgctaga cggtttggag tcacatagct atgatgtagt ctatctccgt cagcctctta
     6061 atcgtctcga gtatattgag tgtgcgatag tggggcaatc acaatttctt tttaaggtca
     6121 gttatgctga tggtcaaaag gcttaccgtg tcgatcttcc tgacctacta acaaagacag
     6181 actggcagat tatcaagtca tttttagatg ctttgcttgc ttatacaggg actgatattg
     6241 aagggctaga tggttttgat tttgaagctt atttccaagc aagtattcaa gcctatctag
     6301 cagaccctgt agctcgtttt acgatttgcc aaggaatttt taatcctatt ttctttagtc
     6361 gtgagaactt gaaaagcttt ttagaggcag atggcttggc tcagtttgaa gcgcgtgtgc
     6421 gtgcggttca agagacagat gcctactttg cgagagtttc cttctatcag gatggagaag
     6481 gaaaagtgca tggcgtttac catctagctc aaggagtcaa gacagtttta ccgagagaac
     6541 cgtttgttcc tgcagcctat attgagcaat tggtggataa ggaagtgcag tgggagattg
     6601 acttggttca aatcacagga gatggctcta aaccagaaga ctatgaagcc attgcccgct
     6661 tggactatgc aaaattctta gaggtattac ccctatcttt ttaccaccaa ctagacgcca
     6721 atcaaataga agtgcaaccc atattagaca aagattttaa agcattagca caagaagagt
     6781 aaagcagaag caggtcaatc gacttgcttt tttgaaatag aaaaatcctg ccatgaaaga
     6841 caggattgct actcaatgaa aatcaaagag caaactagga agctagccgc aggctgtact
     6901 tgagtacggt aaggcgaagc tgacgtggtt tgaatttgat tttcgaagag tatgaagttt
     6961 aaagaaaggc caagatacga agataatctc caatcagtgc cacttcagct acaaagaaga
     7021 ggaggataat aactccgttc acaaggacag acaagaataa ttgatagaag gagtcggttt
     7081 cacttgcttg acttggtctt gtaatgatat ggagactggc aagcagaatg attccaatgc
     7141 taatcacaca caagagggct gtaaatcgta ggctatcaaa gaaagcaaag aaactagcaa
     7201 tagcagtgag gaagattgga attgctaaga gttgactata ttgttggaga accttgtcta
     7261 gcgtccagtc cttttcctgg tggataaatc gtctcacaac gaaactaccc aagaggaatg
     7321 aaaagaagaa gagtgttgtc gctactagga tagagatgat agaaaaaaga gttaaaggag
     7381 ctagctgctc agggaagcga ctgttaatgc ttgctatatg tccatagtaa gcatgtttga
     7441 tgtgatagat actaaagaaa aaggaagatg cagaaaacag aatgagcaag agaaaggctg
     7501 tgtaactgtg tgtgatactt gtttccaact tacttgtagg agatttgatc gcttccacta
     7561 gccaagacca aaaatcaagc acttgctctt tccatttatc cctagatttt ggagcttggt
     7621 cggggatata aggactttct aaagatttac tgataagaag tggctctttc gtggttgctt
     7681 tttgctgagg aagagcttct tggctctctt cagctatagt gactttttct gtttctttag
     7741 aaaggtctgg ctcttcttca gtagaattag atgccttctt ttcttctatt tctgttctcg
     7801 cttcactgtc ttcaggagct tcaattttct cttcttgctg gctttccaat tcgacttcag
     7861 cttgagggac ttcctcctct aactgagtat ttttttcaat tggtgtatcg agatcggcta
     7921 tcgtttcttc agccttgtct gcaacctctt gagcttgctc ttcaggcttg ttcttgcttg
     7981 ttgtttttac aaaatcatta ctttcaaacc attcttgttt catggtagaa cctccttttt
     8041 agttagataa atatgcttcc atagtagcag atgtaagcgt ttttgtcaac gtctgcttgg
     8101 tgtggatatt agatcaatat tatcatcaga tctcgcaatg agttgatcct tgacatcggt
     8161 tttttcagtt ttgtaagggt tgcttaattc cgtacctctt gattcaggct tttctcttgt
     8221 gaattggaag atagaaccat agttgcttga gatgtcccag ttaattcgtt ggctttcttt
     8281 ctggtttagg atgattctga gataatcttt ggcagtcagt tcaaccttgc catggacttg
     8341 gatattttca gcgtggaagt gattctctgt tgactctagc tgactatctg taagaactgt
     8401 atcaaagata ttaacgatat tgggcgttgt gagtttactg tttttgatac gacttccttc
     8461 aattcggagg atatagctgt ttgtattgag ggtcgcattt tcaaggctag catttatgat
     8521 ggtggtttgt ccgcgattgg ctgagatgtt gatccctttt agagttctcc cttttggtag
     8581 ctggagaata acttcttcaa aacgactaga gtagctactt gcgatatgaa gaatcccacc
     8641 aattccagaa gagagaaacg gagtttcaga cagtttctta tcagtgagac tcagagttct
     8701 atcgttctga ttggtgataa gatcatggtg agcagaaaga gatggatggt aagaaatgtg
     8761 gatttgatca tcgaaagagt ctgtgatggt gagcgcgtgt tggtggagag taatttctag
     8821 gttttcgact tccttgccaa aggttagctt ttccgtacgg ctatcataga taggttcttt
     8881 ggacatggaa agtaggcttt taatcccgtc agattggata cctacaaaaa gaaggataaa
     8941 gccgataacg gtagtcacca caccaaagat gagaaatcct tttgtccatt tacgcatgct
     9001 ggttacctct ctttcttttt ttaagaacaa attgtaccag gcgaacaatg agtagaccga
     9061 agaagcgagc tacataggaa atgcctagta aaactagcga agaagcaccg atagccagta
     9121 aaccagaacc aaaaatcaag ataaaggctg atttggcttg ggcgaggaca gtgaaacttt
     9181 caactaaaaa taggaatccg ccgatgatac ccagtatgga aactgcaaag aaagccagaa
     9241 tgacagtcaa agcggctaca aggattgcga acagggtcac gaggatggcg attcccagag
     9301 gaatgccgat aggtgctgca aggagggcta acaaggcgat atgtaaaatt tgtcggttat
     9361 ttttttgagc gggtgcttca ttgatttttt tatcgagaag attggataga acttcgtggg
     9421 ccgcttcttt gggagttccc aaactagcga tgagttcttc ttctccttcg actccagcat
     9481 cgtcaaagag ctctctgaaa tagtccatgg cttcgatacg gtcagcttca ggtagtttct
     9541 tgagatagag ttctagctga gtcaggtatt cagttcttgt catggcggat actcccttct
     9601 atgatgccat tgatggtgtc tgtatagagt gcccattcat cttttagggt caagagctgc
     9661 tctataccac cgtttgtcaa ggagtagtat ttgcgcatgc gaccttggaa ctctctagaa
     9721 taggttgtca gaaagctatt gccttccaat tttttgagaa tgggatagag tgtggattct
     9781 ttgatattag cgatcagctt aatggtttgg ctaatctcat aaccataaga atcaccctgc
     9841 tccagtacgg ccaagatgag aaattcaatc aaggcagagg atgttggaaa gtacatggga
     9901 aacctccttt tctaatgtgt aagattttta tatataattt ttctacacat acattgtaca
     9961 tctaaaagaa agccctgtca agagaaatgt gtaaaatttt tatatataaa aaacttctag
    10021 ctaaaactag aagtttaaag gatcttatcc gctctgtcca ctgtaaagag ggccacagtc
    10081 atcaggatat cgatgagcaa gagggcagct acagctggta cccaagagtg gaataggtca
    10141 aaactgtaac caaagagggt tggcccaaag gctgctagga tatagcctcc tgtttgagat
    10201 aggccggaca attgggctgt cttttcaggg gcgcttgtct tgagtgaaaa gttgaccatg
    10261 agataaggga agagggcgct ggttgcggtt ccgatgagga gatggatggc aagccagtaa
    10321 atgaaattat tgattgggaa aaagagcatg gaaatgccga ccacaccagc tagtgaaacc
    10381 agagtgagca tgagctgacg gttgcgagta gataaactgg ttgtcaggct tgggatggtc
    10441 attgaaaaag gaatgctaat cagagataag atagaagtca gcaagccagc ttcgtgactg
    10501 gatagacctg catggataga catggtaggt aaccaggtca tggcggtgta aaagatcaag
    10561 gattgaaaac ctgaaaagat aataattgcc caaacctgtt tattacgcat gacctttatt
    10621 tgactttttt gtttggtttg tggagctagt ctatgattat agcggtgatt tgggagccag
    10681 accaaaaaag ttgctagaca gagtaacgtg aggaggagga taagtccttt ccaagaactg
    10741 gcttgtgtaa tgggcacagc tagataggaa gccagagccg ttgcaatccc catagacgtt
    10801 acatataagg tggtcagaaa accaattttc tttggttgat tggcttggat aagactagga
    10861 agcaggacat tgatgactgc gacacttgcc ccaaccatca aggttcctag atagagcagg
    10921 ggcagattga ttagtcgaat aagtgagccg atggtcaaga agaagaggct gtaggtgaag
    10981 agatgctcca agccgatttt ctgagccagt cgggtagaaa atggtgaaaa gagggtaaac
    11041 ataaggagag gcaggctggt caagacacca agcgaactaa cttctacctc cagaccttgc
    11101 gaaatatttc ccaaaataat gggtaaaaca gtaaaaggag ttcgcaagga aacaccaatc
    11161 aggataatac ctggaacaaa aaagagtgat tgctttttca tataatatct cttctagctg
    11221 attttaataa cagcttattt taaggctttt tcactataat gtcaagtaag gtttctggct
    11281 ttcaagataa aaatggaaaa gtcttgaaaa ttatgataga atggtggaag ggaaaattca
    11341 ggagagtagt agtgactcaa aatgttgaaa gtcttctcgt atccattgta atcagtgcat
    11401 acaatgaaga aaaatatctg cctggtctaa ttgaagactt aaaaaatcaa acctatccta
    11461 aagagaatat tgaaattcta tttataaatg ctatgtccac agatgggacc acagctatca
    11521 ttcagcaatt tataaaagaa gatgcagagt ttaactcaat tagattgtat aacaatccta
    11581 agaaaaatca agctagtggt tttaacctgg gagttaagca ttctgtaggg ggccttattt
    11641 taaaaattga tgctcattca aaagttactg agagttttgt aatgaacaat gtggctgtta
    11701 ttcaacaagg tgaatttgtc tgtggagggc ctagaccgac gattgtcaaa ggaaaaggaa
    11761 aatgggcaga gaccttgcat cttgttgagg aaaatatgtt tggcagtagc attgccaatt
    11821 atcgaaatag ttccgaggat agatatgttt cttctatttt tcatgggatg tataaacgag
    11881 aggttttcca gaaggttggt ttagtaaatg agcaacttgg ccgaactgaa gataatgata
    11941 ttcattatag aattcgagaa catggttata aaatccgcta tagcccaagt attctatctt
    12001 atcagtatat tcgaccaaca ttcaagaaaa tgctgcatca aaagtattca aatggtttgt
    12061 ggattggctt gacaagtcat gttcagttta agtgtttatc attatttcat tatgttcctt
    12121 gtttatttgt tttgagtctt gtgtttagtc tagcattgtt accgatcaca ttcgtattca
    12181 taactttact attaggtgcc tattttctac ttttatcatt actgtcatta ctcactttgc
    12241 tgactttatt aaaacataaa aatggatttc taattgtgat gccctttctt ttattttcca
    12301 ttcactttgc ttatggcctt gggacgattg taggtttaat tagaggattt aaatggaaga
    12361 aggagtacaa gggaacagta atttatttgg aaaaataagc caaataaatt aaaatgtgct
    12421 ataataacaa tatagtagaa ctattttaag gaggagtaaa tttctatgaa taaaaaactc
    12481 acagattatg tgattgatct ggtggaaatt ttaaataaac aacaaaagca ggttttctgg
    12541 ggaatatttg atattttcag tatggtggtt tccatcattg tatcttatat tttattttat
    12601 gggctgatta atccagcacc tgttgactac attatctata cgagtttggc cttcctgttc
    12661 tatcaattga tggttgattt ttggggcttg agcgcgagta ttagtcgtta cagcaagatt
    12721 acggatttca tgaaaatctt ttttggtgtg actgctagca gtgtcttgtc atatagtatc
    12781 tgttatgcct tcttgccact cttctccatc cgttttatca ttctctttat cttgttgagt
    12841 gccttcttga ttttattgcc acggattact tggcagttaa tctactccag acgcaaaaaa
    12901 ggtagtggtg atggagaaca ccgtcggacc ttcttgattg gtgccggtga tggtggggct
    12961 ctctttatgg atagttacca acatccaacc agtgaattag aactggtcgg tattttggat
    13021 aaggattcta agaaaaaggg tcaaaaactt ggtggtattc ctgttttggg ctcttatgac
    13081 aatctgcctg aattagccaa acgccatcaa atcgagcgtg tcatcgttgc gattccgtcg
    13141 ctggatccgt cagaatatga gcgtatcttg cagatgtgta ataagctggg tgtcaaatgt
    13201 tacaagatgc ctaaggttga aactgttgtt cagggccttc accaagcagg tactggcttc
    13261 caaaaaattg atattacgga ccttttgggt cgtcaggaaa tccgtcttga cgaatcgcgt
    13321 ctgggtgcag aactgacagg taagaccatc ttagtcacag gagctggagg ttcaatcggt
    13381 tctgaaatct gtcgtcaagt tagtcgcttc aatcctgaac gcattgtctt gctcggtcat
    13441 ggggaaaact caatctacct tgtttatcat gaattgattc gtaagttcca agggattgat
    13501 tatgtacctg tgattgcgga cattcaagac tatgatcgtt tgttgcaagt ctttgagcag
    13561 tacaaacctg ctattgttta tcatgcggca gcccacaagc atgttcctat gatggagcgc
    13621 aatccaaaag aagccttcaa aaacaatatc cgtggaactt acaatgttgc tagagccgtt
    13681 gatgaagcca aggtgcctaa gatggttatg atttcgacag ataaggcagt caatccacca
    13741 aatgttatgg gagcaaccaa gcgcgtggcg gagttgattg tcactggctt taaccaacgt
    13801 agccaatcaa cctactgtgc agttcgtttt gggaatgttc ttggtagccg tggtagtgtc
    13861 attccagtct ttgaacgtca gattgctgaa ggtgggcctg taacggtgac agacttccgc
    13921 atgacccgtt actttatgac cattccagaa gctagccgtc tggttatcca tgctggtgct
    13981 tatgccaaag atggggaagt ctttatcctt gatatgggca aaccagtcaa gatttatgac
    14041 ttggccaaga agatggtgct tctaagtggc cacactgaaa gtgaaattcc aatcgttgaa
    14101 gttggaatcc gcccaggtga aaaactctac gaagaactct tggtatcaac cgaactcgtt
    14161 gataatcaag ttatggataa gattttcgtt ggtaaggtta atgtcatgcc tttagaatcc
    14221 atcaatcaaa agattggaga gttccgcact ctcagtggag atgagttgaa gcaagctatt
    14281 atcgcctttg ctaatcaaac aacccacatt gaataaaaaa gaaaaacgca tattatcaag
    14341 ttaaataacc ttggtaatat gcgttttatt atgtagagac ttatactctt cgaaaatctc
    14401 ttcaaatcac gtcaacgtcg ccttgccgta tatggttact gacttcgtca gttctatcca
    14461 caacctcaaa acagtgtttt gagctgactt cgtcagttcc atctacaacc ttaaaacagt
    14521 gttttgagca gcccgcagct agtttcctag tttgctcttt gattttcatt gagtattact
    14581 tcattttctt ctgaaataga gttgtcgccc agtctatgct attgaaaata cgccaaaact
    14641 tctaagggtt tgtgagcgat ataatcaggt tgatagttta gtagatctgc ttgctctcca
    14701 aatccccaag tgacggccaa tttctgaata cctgtttctc gagctcccag catatcaaac
    14761 ttggtatctc cgatgatgat ggcttgttct ggtgctagtt gatgtgtctg taaggcttgg
    14821 tgaatgacat ctgccttatg gggtgcttca gggctagaac cataaatacc atcaaagaaa
    14881 tgatggattt ccaagttttt ggccatgtct tgagcagtag atgtattctt tgtcgtggtg
    14941 atgtagagtg gataactgct cgataactcc tcaagcaagt ctataatctg aggaaagagt
    15001 tgagcttcat agatgccttt tgccttatag taagaacgat atatctgcat ggcttcagaa
    15061 atttggtctt tggacaggca ggtcgcaaaa ctactttcga gaggtggtcc cataaaacca
    15121 cgaatagttt tggcatcagg gctaggcacc cccagctctt taaaagtata ggtaaaggca
    15181 ttgtgaatcc cgatagaact atcaacgagg gttccatcca aatcgaaaaa aatcgctgtg
    15241 atagaggtca tggtttctcc tatttgataa gcttattctc cgaaaatctc tttttggagg
    15301 cgacgaccag taggggtggt agcgagtcca ccttcagctg tttcacgaaa ggcagttggc
    15361 atgcttgctc ctacttggta catggcatcg atcacttcat ccacagggat tttagattcg
    15421 atacctgcca aggccatgtc tgctgcgatg aaagcaaagc tagctcccat ggcattacgt
    15481 ttgacacagg gaacttcgac caaacctgca acagggtcac agatgaggcc tagcatattt
    15541 ttaatgacaa aggcaatagc ttgactggcc tgataaggtg ttccacctgc agccagagtc
    15601 aaggcggcag cactcatagc agaggctgaa ccaacttcag cttgacaccc accctcagca
    15661 cctgagatgg aggcattgtt tgcgatgact agtccaaagg caccagcagc aaagaggaaa
    15721 tccaattgtt gctcgtggct gaggtctaat ttttcaatag cagcagtgag aacggaaggg
    15781 agacaaccgg cacttcctgc ggtaggagta gcgcagacca agcccatttt agcattgtgt
    15841 tcattgactg cgatagcatt tcgggcagca gagagaatcg tgtaatctga taaagttttt
    15901 tcgtttttga tgtagtgatc caatttggca gcatctccac ctgtcaggcc actacgagat
    15961 ttattttcat tgaggccaag ttggacagag gctttcataa cttccagatt gcgttccatg
    16021 agaaggaaga cttcttcacg ttcgcgaccg gtcaattcaa actctgttgt aatcatgagt
    16081 tctgcgacat ttccttgaaa gtccagatct gcttgctcga ccaattcttt gatagaataa
    16141 aacatgcttc ctcctattta aagaaattga cattgtggag atgagggatt tttcgaattt
    16201 cttcgatagc ctcatcacag ttgcgactgt caacttcgat aatcataatg gctttttcac
    16261 cagctttttc acgagtgaca ttcatctggg cgatattgat accatagcgg gaaagcgcct
    16321 ctgtaacaag ggcaatcata cctggaatat cttgatgaac gatgatgata gtcggtgtat
    16381 tcatattgag agagacggca aaaccattga gttcggttac ctgaatattt cctccaccga
    16441 tagaaatacc agtcacgctg atggtcttgt gggcattttt aacagtaatt ttagtggtgt
    16501 tagggtgagg ggcattgctg tctttctgaa tggtccagac aatcttgata ccacgcttgt
    16561 gggcaatttc cagactattt ggaatttcag gatcatctgt atccattcct aaaatacctg
    16621 caacaagggc taggtctgtt ccgtgaccac gataggtctt ggcaaatgag ttaaaaagtt
    16681 ggaattcaac ttctgtcgga gtatcatcaa aaatggaaga gacaatcttc ccaatacgaa
    16741 cagcaccagc ggtatggcta ctagatgggc caatcataac tggtccgatg atatcaaaga
    16801 cagattgaaa acgaagtgat ttcatcagtt tccccttata aaaattctta tctctattat
    16861 atcaaagaat gaggggcttg gctttaattg tggatgaaaa cctttctaat acctcaaata
    16921 gcataaaaat agtatctttt atgacaaaaa acaccttatt tagggaaata aaaaataatt
    16981 ttgtaatatt tctacataaa agtgtcaaga aacggtaata tttaaagggt atgatagaac
    17041 tatagaaaga aggagaattt tcgaatatga aatcaataac taaaaagatt aaagcaactc
    17101 ttgcaggagt agctgccttg tttgcagtat tttctccatc atttgtatct gctcaagaat
    17161 catcaactta cactgttaaa gaaggtgata cactttcaga aatcgctgaa actcacaaca
    17221 caacagttga aaaattggca gaaaacaacc acattgataa cattcatttg atttatgttg
    17281 atcaagagtt ggttatcgat ggccctgtag cgcctgttgc aacaccagcg ccagctactt
    17341 atgcggcacc agccgctcaa gatgaaactg tttcagctcc agtagtaagt gaaacagttg
    17401 tttcaactgt aagcggatct gaagcagaag ccaaagaatg gatcgctcaa aaagaatcag
    17461 gtggtagcta tacagctaca aatggacgtt atatcggacg ttaccaatta acagattcat
    17521 acctgaacgg tgactactca gctgaaaacc aagaacgtgt agcagatgcc tacgttgcag
    17581 gacgttacgg ttcatggact gctgctaaaa acttctggct taacaatggc tggtattaag
    17641 aactaacgaa caaataatat gtaaagagtc gaggaaatcc ctcggctttt ttatctttcc
    17701 ttcgcttggt aatagatctc tacttctttt ttgagatgag acagcataga agtttcctcg
    17761 gttagctcca gaagggagaa ccctttttgg ataagtttag aatcgtctgt acaaatccca
    17821 atacggacat agcagatagc cagcctatcg tagcttttct tattcttggc gtcctctagt
    17881 aaagtgtagc agatttgctt acacatgttt ttatcttgat tgtatagata gagtgtggaa
    17941 aggttgagca ggattgctat ttgcaagtca tataaatgtt gatagttttt atagacttct
    18001 aagcgttgca agattttctc ggattattga caaataaacg gacattctga tagacgattg
    18061 ccctagcggc ttagtgtaaa ctttacaaat ttggcttgta atttttttat aaaattcact
    18121 tcagttactt gacagttatt atattttcac ttattatata gataaaagga tgagatggga
    18181 gtttaaaaat ttgaaagcgc tatccatatt gtttattgga ctgattcttg agtaatagtt
    18241 agagtgtgct taatactact ctggttgttc aaggacctga tacagaagcg caccattctc
    18301 tcggttaaga gtaagttcgt tgttaaaaaa tgaaatgaga tagaaggaag gtgagtccaa
    18361 tggactacga tacttgttga taaattacta tagctgtttt tacggaagag ctctcgataa
    18421 taggagaaat aaatcatgag aaaattagtt aaaattggtg ttgcatcttt aatggtatgt
    18481 agtatacttg cttctacaag tgttttggca gtatgggttg atggtggtca atggaactat
    18541 ggagtaggat ggtcaggaaa ttttggttat tctgattatt tacattctac tcgctctcat
    18601 acagcaactg ttaaagatgg aaataaattc tctaaggatc gtgcagaagc tgaggcatgg
    18661 gctcgggctt caatcttcaa attcccacca acgggaatgg aatatttcta tggattttaa
    18721 ttaaaattct ccctcccctt ttggagaggg agtttcttca cgggaggtat ctctatgaaa
    18781 aaaatcagtc atctttgtat gttactcctg cttctgtgta ccactttttt tgtttttaat
    18841 gtaaactata cacgagaagt ggttcggatt caagaaatgg gaaagactgt agattctttg
    18901 gatttgtatt tgaaagatat taacgaacct gcagcgtctg ttcttcgatt ttttgaggat
    18961 gtatcaaagg agtataaagt ctccatcatc aaaacagaca gtggtgatga ggtggtcaag
    19021 tctggtgttt ttgataaaga taccttcccc taccaagagt ttgggatttc ttctcttgat
    19081 tttaccacag atggtgaagg agtctatagt aataaagaaa tttccaataa acttggtacg
    19141 attccgacct ttctaaaagc caaacctatt cagcttatga cttttcaaac ctatatcaag
    19201 gatacatctc gtagtttaaa tggtcgctat acgataactt ctacacaaga gatggacaag
    19261 gataggattg tacagaaatg gagcgatttt ttcaagatag accaggctac cttgctagag
    19321 ccgacctaca aaagtgcagt ggaagtcata aatcgagatt tgcttttatc tgccattgtt
    19381 tttgtcttgg ctattttgct tcttgtgtta gtgacagtgt atcaaccgat gatggagatg
    19441 aaaagagttg gggtacaaaa attacttggt tttcaagata gggctgtttt agctgatgtt
    19501 gtaaaaggca acctttacct cctcctaggt ggggctcttg tgatcaatct aggcgtgttt
    19561 ttcttgcttg attataagcc aaaagatttg tttcctatgc tgtggttgtc tcattttttg
    19621 ctgttgcagc tttatctctt tatcagttgg ttgacttacc tcttaatcca aaaaatgaca
    19681 atcagctctc tgctgaaagg tttttcatct ttcaaatttg gtctactctt taattatttg
    19741 atgaaaattg gaactaccat tttactgaca gtcttgctag ttggggtagg aaaaagtttg
    19801 gagcaagaaa acaaagagtt ggattaccag aaacagtgga tcagccaagg aaattatctg
    19861 accttagaaa ctttccaact caatgataac ttgtggcaag agcagttggc aggctcaggg
    19921 aaggcattgg attatttcta tcgattttat caggatttgg tagaaaaaac gcaggcgggc
    19981 tatgtgcaga gtagcagtct tcctgtaaaa aattttgtcc aatcagaaca gattcagcaa
    20041 tatcagttaa cagatacggt ggatgtttac tatgccaatc gcaattttct aaagagcaag
    20101 ggattcaagc taccagatac cggtattaaa aaagttattt tgatgccagc aagtacgaaa
    20161 ggtgaagaag ataaaaatca gctcttgggg aagttaattg cctttcattc gatgaagtat
    20221 gaagagcagc aaaaacgaac gatagaggag atggatgtcg agattgccta ttatgaagga
    20281 gattggtcat ttttcccata tagtgataag cgaaaggaaa atctctccaa tccaattatt
    20341 agcttggtca atgattctga tatgatgtgg gatgagaaag cctccctgtc aacaactggc
    20401 ttaaataatc cgattaaaat tgaaaatacg gttcaacatc aaaaagagat tacagagtta
    20461 gttgagaaat tgtcagatgg aaattattta aaattttcat ctattcaagc cattcaacaa
    20521 gagaaagtgg attcttatcg agatgctgtt cggaatttta acctactctt tgctttgttt
    20581 ggtctcctta gcatgatgat ttcctacttc ttactagtaa taactttctt attgaagcgc
    20641 agggatatca ttaccaagaa gtttatgggg tggaaactgg tcgatcgcta ccgtcctctc
    20701 ctcgttctgc tcttgctggg ctatagtttc cctcttctag tcttgatttt ctttgcccat
    20761 gcgttcttgc cacttctgct gtttgcaggc tttacatgtt tggatatact atttgtgcta
    20821 gtcttggctt ctaggatgga gaaaagaagt ctagtagagt tattgaaagg gggcatctta
    20881 tgattgagtt ggaaaatatt accaaaacca ttgggggaaa agtgattttg gatcacttgt
    20941 ctctcaggat tgatcagggg gatttggtag ctattgttgg aaaaagtggt agtgggaagt
    21001 cgaccttgtt aaatttatta ggtttgatag atggtgatta tagtggacgg tatgagattt
    21061 ttggtcagac aaatctagcg gtcaattctg ctaagtcgca aacaataatc cgtgaacata
    21121 tctcttatct gtttcaaaat tttgccctga ttgatgatga aacggtcgag tacaatctca
    21181 tgctggcgct gaaatatgtg aaattgtcta agaaagacaa gctcaaaaag gtggaagaga
    21241 ttttagagag agtaggtttg tcatctactt tgcatcaaaa ggtttccgag ttgtctggtg
    21301 gcgaacaaca acgaattgca gttgctagag ccatcttaaa acccagccag ctgattttag
    21361 ccgatgaacc aacaggttcg ctagatcctg aaaatagaga tttggtcttg aagtttctct
    21421 tagagatgaa tcgcgagggg aaaacagtca ttattgtgac ccacgatgct tatgtagccc
    21481 aacaatgtca tcggatcatt gaattgggcg aggggaaatg agtttgttca gctctttttg
    21541 actgactgaa tactcatgtt tttcagagag gaatagcata aatacgccta gaaatgacat
    21601 ttttatgtag tatttctagg tttttttgtt tcaaattgaa aatttttcca atttaggctt
    21661 gacaaaggat gaagataggt gtattattta tacaaccaaa aagaataaac ataaaggaga
    21721 ctttgttatg aataagatga agaaggtgtt gatgacgatg tttggtttag tgatgctccc
    21781 cctactattt gcttgtagca acaatcaatc ggctggaatt gaagccatca agtccaaagg
    21841 aaaattggtt gtagccctca atccagattt tgctccattt gaataccaaa aagtggttga
    21901 tgggaaaaat cagattgtgg gttcagatat cgaattagcc aaggctatcg caacagaact
    21961 aggtgtcgaa ttggaactat ctcccatgag ttttgataat gtactggcta gtgttcaatc
    22021 aggaaaagcc gaccttgcca tatcaggtgt ttctaagaca gatgaacgga gcaaggtgtt
    22081 tgacttttcc attccctact atactgcaaa aaataaactc attgtcaaaa aatctgactt
    22141 gactacttat cagtctgtaa acgacttggc gcagaaaaag gttggagcgc agaaaggttc
    22201 gattcaagag acgatggcga aagatttgct acaaaattct tccctcgtat ctctgcctaa
    22261 aaatgggaat ttaatcacag atttaaaatc aggacaagtg gatgccgtta tctttgaaga
    22321 acctgtttcc aagggatttg tggaaaataa tcctgattta gcaatcgcag acctcaattt
    22381 tgaaaaagag caagatgatt cctacgcggt agccatgaaa aaagatagca aggaattgaa
    22441 agaggcagtc gataaaacca ttcaaaagtt gaaggagtct ggggaattag acaaactcat
    22501 tgaggatgcc tttaaagcat ccattgaaaa atagaaagaa ggaaaagaac atgagtaaag
    22561 aaaaagtaat tttggcctat tctggcggtt tggacacatc agttgctatt acctggttaa
    22621 agaaagacta tgatgttgtt tcagtttgta tggatgtggg tgaagggaaa gacttagact
    22681 tcatccatga taaggctctc aaggttgggg cagtcgaatc ttatgtcatt gatgtcaagg
    22741 acgaatttgc tacagattat gtgctagtgg ctcttcagtc acatgcctat tatgaacaaa
    22801 agtacccctt ggtatctgcc ttgagccgcc ctcttatttc taaaaaatta gttgaaatag
    22861 cgcatcagac aggagcgact acaattgctc atggctgtac cggtaagggg aatgaccaag
    22921 tcgaatacca gattgcagtc gctaagaaag cgaatgaagc taaaaagtga tggagtgatg
    22981 ggttcagaat aagaggttgg tcgattggta gcctctttct tgtcgttgaa aaagtgagat
    23041 atattgactt ttgaaaaaaa tgtcataatt gagttgggat aaacgtaaaa gtcatacttt
    23101 ctcgttaaat cgttcggcga ttcatggtga catggacaca tagtagtaaa cacactatgt
    23161 ggcttggcag agctaaaaac ttctggctta acaatggctg gtattaagaa ctaacgaaca
    23221 aataatatgt aaagagtcga ggaaatccct cgactttttt tataatttct ttggttgata
    23281 atgggtctct acttcttttt tgagatgaga cagcatagaa gtttcctcgg tcagctctag
    23341 gagtgagaac cctttttgga taagtttaga atcgtctgta caaatcccaa tacggacata
    23401 gcagatagcc agcctatcgt agcttttctt attcttggcg tcctctagta aagtgtagca
    23461 gatttgctta cacatgtttt tatcttgatt gtatagatag agtgtggaaa ggttgagcag
    23521 gattgctatt cgcaagtcat ataaatgttg atagttttta tagacttcta agcgttgcaa
    23581 gattttctcg gattattgac aaataaacgg acattctgat agacgattgc cctagcggct
    23641 tagtgtaaac tttacaaatt tggcttgtaa tttttttata aaattcactt cagatacttg
    23701 acagttatta tattttcgct tatgcttatt atatagataa aaggacggga taggagttta
    23761 aaaatttgaa agcgctatcc ataataataa cgcataattt tctccttttc gtgaaaaaag
    23821 ttaacgtaat tttaaaatga gcttaataag aggtactatg taggtagaaa ataaagatag
    23881 gagaaaaagt atgaataaaa aaatattttg tattctggta tgtattatat cattgattag
    23941 tctagctata atctttccat ggggctggtc gatataatgt aagggagctt gagttgttag
    24001 tggaggataa aatgttaaga gtaatagtag ttattcaatc tttagtaata atactgcttg
    24061 ctgttgcttt attattatct cggaaaaact ttgattcgtt aaatgttgtt cttataatga
    24121 tgatcgtatc acaattgttg ttattagtat atttgataaa atcagggaat cagtgattag
    24181 ctgttatttg aattaagtaa actgttaagc ttcaagcagt aactggtttt aaatatttaa
    24241 aataagataa aggaaggaag tctaatttag gtaactaaac aaagcctgcg atcacttgtt
    24301 aggaaactat cctaaccatg cttaataaaa aaggagaaaa taaatggaac tcgtattacc
    24361 aaataattat gttgctcttg agcaagaaga gatgatgtat cttgatgggg gtggtgttgg
    24421 tcgtaactgg tggaatagta gaggtagttt tgcaacagtt ttggatgtag gtttggccat
    24481 ctatagtggt ggtgcaacaa tttattctgc ttatgcgata aaaaaagcta tctcagctaa
    24541 tagaggggct attacgagaa cattacgtag tttaataatt aaacatgtag gtagtgcagc
    24601 tggccattta gtcaatactg cactaaacgt tgcactaact gttactggat tttcactagg
    24661 tggagcaatc gcatatgggg ctgattgggc tgacggtagc ttagatggtt atatttttgc
    24721 ttaaaatgaa acattttttt gcaggaattg gtgaaattca ttttttgtta tttttattat
    24781 atctaagcat gtctgctagt ccaattattg gactagtgat tggatatact aaaataaatc
    24841 taataaattc tattattata gctcttggta tgatattggc attaattttg gtattatcaa
    24901 gagtgtatag aaaatatttt tatagagacg atgatgacta aagtttttat caataatttg
    24961 ggctccttgt caactgtagt gggttgaaga aaagctaacg tctggggaag gggttaaagc
    25021 tttgctttat caaatattga cttacagagt tgctctattg tattgatgta ttcttagtaa
    25081 ataatatggt gttttgaatg aaagatgatc agaaatattt attagcggga ctttattcgc
    25141 ttctagttgc aatattttat tttccattga ttgagtccaa ggggattttt gtaagtatat
    25201 tgatggcggt tttactttta tatttaatat acttcattgg aacagtcatt catattgtga
    25261 taattaagtt tataagaaaa aaatctttta agtatttggt tctatatcca tttacttatg
    25321 atgggagctg gagatttcaa cctataaact tactctactt tccagaaatg gtgagagatg
    25381 tcattccgat taatttagtt caagaatatt gccaaggaca accttacggg ctgttaaaaa
    25441 agatgctgaa aagaattcga ttgtcacgag agatagcttt actcctggct accattatcg
    25501 tctatttttt tacgcataga attctacctc tttcagtatt tacgttcatg ttttcttaca
    25561 tattgatatt tgcacaatct tatttgggtg gtaatactgc ttggattgga aatagaagat
    25621 taataataga tgatgaattt gaaaaaatct tgctttcaaa aagttatata aaagaaatat
    25681 catctgcacg atactcggaa tacttgacat gtgaatacaa aaaccttaca ccaatcatat
    25741 tgttagctat ttttgaaaat ttacttgact cctacttgct tcagaatcag agtgaggttg
    25801 acttagatat tttttataaa gttttacctt tgctttataa agaaaaatat actatgggat
    25861 tcaattactt tgtaagttta aattatttac tatataaggt tggatttttg ggaatcattt
    25921 acgataatga agcattagga gatttgtcaa aacaatattt aaacagaaat atttcggaat
    25981 tacaagatgg ttctttcgag ggcggtatac aagatgctgt tgcctctaaa caaatagtag
    26041 ttataaatga atttatagct tgtttaaatt ccagatgcat gccatcccaa tatgatagat
    26101 ttttttataa agatagacct tatatttttt ccagaaagag tcctattaag aggtgaaaat
    26161 ttgttacatt tggattaaca agtgtcctag ggattagaaa taactctagt atagctagat
    26221 atttttgagt aactagaaat atcattaggc tacccatatg attgccagaa aagatggctg
    26281 atgaatctta tttatactgt tgcagtaggc gttgtctagg ataaataagc tatcttgatg
    26341 ggggagattg gaataatttt agtagaaaca ttaaaggatt atgggcaagt agcacaggat
    26401 taggtgatgc aatgtgctaa gttggcttgg tgttagcagt agtattattt aacaatggaa
    26461 agtagactaa atgaaaaata aacgttattt ttttgacaca attttaatga ttttactact
    26521 tataagcacg atattttgtg tcagcccagt atttataaaa ttggacatct taggaactcc
    26581 ctctcatgcc atcttaacct ttgtattggc gataccgtta ttctatattt tgtctcaatg
    26641 cttacatact ttgctattac tagtttcctc aatcttttgt aaattaagac caatttattt
    26701 ttattttata tttgtgatca ttataggagc taggaaatac tatcgaattc tctttcacca
    26761 actaatggga tttagtccag gaatagcagt gttttataag gagagtcaaa caactaagaa
    26821 tctgtttaaa ttttactact tcttgtactt tacaacacta atttcatact attttttctt
    26881 tacttttgtg tatgacaaac cgttgctttt gcctcttatt ccattttcta ttatcatagc
    26941 attagtacaa aaactttata gaattgagaa tcagcaatta tttctgttaa aatcaaaggt
    27001 actaacaata ctggaatcta agaagaattg tgagtttaat cttcaggatt atcatgaaat
    27061 ttggaaattg caatcaaaat ctgagttgcc atgcgtggct ttatcttata tcagtttgat
    27121 caagccatat ctttcagaga gcgtgcgtga acaaattgat ttattagaag ttaagcgatt
    27181 taaaaaaata aatcatccaa tatcacttta tggtatgttg gatgttataa aattaaatct
    27241 atatttacgg cattataatg aaaaaataaa tatgaatcaa tgttaaagaa aattttagag
    27301 gtaagaccag actttgtttt aatcgagcag aatattgatg atagtttaaa tagctcgcaa
    27361 ccattatctt tatctttagc tatttcagaa atccaacttt tgcttgaagt gtatataggg
    27421 atcaaacatg tttcaattag gagataaaac tagaataaca tttgctatta taggggcaag
    27481 ttgcatatta gtcagtatgt atcttatacc tctcattaac acggataata aattttggga
    27541 aatactttta tttataatag gtgtattgct aattggaatt gcgttactaa gtcaaaaaaa
    27601 ataaattttg aaatagtatt agtgagttcg caatcattta ttgctagtgg tttagaactg
    27661 ttaagtttca agcaataatt ggttttaaac cttaggtaac ctataaaaac aaataaggta
    27721 gagaaaggat atcttattta ggtaactaag tactataaac acagtcgaca atcacttgtt
    27781 aggaaaccat cctaactatg cttaataaaa aaggagaaaa taaatggaac tcgtattacc
    27841 aaataattat gttgctcttg agcaagaaga gatgatgtat tttgatgggg gattttctat
    27901 tccgagatgg cctgttgcaa cagccattaa tatagctttt aatgatgtct taggtggagg
    27961 agcaatcagt ctagttagaa attatattcg taattatggt ttgcggcgag ttacaagcgc
    28021 aattgctgga gcagctgcaa gatatgttgg ggtacgagtt gcaaatagag tggcaggatt
    28081 tgcactgtct gctattaatg gatttgcagc ttggatgtca attggcaatg ctattacaac
    28141 aatctgggcc aataatgatg taaataggag agacccaaat ttaaacgcct tgtggtaact
    28201 agatatgggt attaaaatgg aaaaaatttt tgttattatt ttttttgtat gtctatttat
    28261 atcatccata acttttttag cctatgattt tgttagcgaa gaaataaaaa agttgattat
    28321 ttggatgaat gtcgtttttt tgattttaat catagcaatg ataatttatc caaaattaag
    28381 aaaatgataa ggacactgta aaattatgat acgtaaacta atcatttttt tattaatgct
    28441 ccctcttttt gtcttgtggc tagagttgca tgtcctagtg aataacttac tattgaatct
    28501 agaatctccc cttgattttg tgattagtat gagtcctgct ttctgtagtt tgattttatc
    28561 aaaaatagtt ttagacatac tatacgcatt gaaagatttg tacaagaaag aagctcttat
    28621 tacgattttt ccttttattt ttataggcag gaagaaagta aatgtgaggt tctcgcccta
    28681 tttttcattc catcgtaaaa gtttgtcgcc tgatgattta agatctagga ttatatggag
    28741 ttttattctt gaaattgcta ttatcctagt atttatttta aaaattcctt ttgcaatcat
    28801 catgcttact acaatatttt tctggactat aatggatatc aatcatctcg tttttaataa
    28861 gacggaattt ctttttaatc aaaacaaatg gcaaaaagaa gattcgtttg aaagtgattt
    28921 gacgaaaacg ttaaaggata agatacaaaa atcagagcta agttattctg acttaatgtc
    28981 tcttcagctg tatgatgcga tgaaccaatc caccttttta acggatagtg aactttttga
    29041 ggatattttg aaaaaaatcg aagattctca taacaccctt ctgtgtacag gtcttgtcga
    29101 gttactgctg tatgaaattt ctattagtaa caataacaac tggcaagaaa aggttgataa
    29161 aattagaata cagctcatca gaatcaatca attagatttt ttctattata caagttggtt
    29221 aaggcaaaat tttgatttct gtatgaatag agagtatcat aaaatgaaat ctcggaaatt
    29281 attactgagt aataaaaaga tagtttaagt tcgtcctatt tgtaaaacaa atgaaacaat
    29341 ttcaactaag gaggagaaaa caaatggaac ttgtattacc aaataattat gttgtgattg
    29401 atgaagaaga aatgatgtat cttgatgggg aagcttattt aagcaagcgt gcttgtcaag
    29461 gaatttgcgc agctttagct atgagttcag gaacttttat agcattagct ggagctgcag
    29521 ttttaaccaa aaaactaata aactatatta aggttggagg tcttggaggc tggcttattg
    29581 gtgcagcagc aggtgtattg gctacagcag cagggaaaat agcttactat attggatatg
    29641 gtgttcttaa tagaggttgt gatattaacg ggaaccctta tccttgggat ggattcatat
    29701 ctgcgacagt aaaatgagta atgtagataa gattcgaaaa atacacatta tcgtatgttg
    29761 gatgtatata tttttatcat tcagggcgat tataaatgac acggagtatt ttttgcttat
    29821 ttttttagct tttatctact caatagtatc actacctcta tattctgtga aaaataaaat
    29881 agtatctatc tgtctagcca taaattcgat tctgttaatg agtttcccca ttttaatcaa
    29941 taagtttttc ccagaaagtt ttttgactta taccgtatta ataagtgttt ttattacaga
    30001 gttgataatt tttcatttaa ttgggaaaga ttttgatatt aaattgacta acgaatataa
    30061 gaaaattagt cagtttagaa gcaaggtgtc tcaatctcct tggataaaat atttagagat
    30121 ttctagtttt atattaacta tatttccatc tattctttat ggtacagttg ataatcatgt
    30181 actgactctt atctttttga taaaaatttg tgcagatacc acgataaaat ttttattcat
    30241 cagattattt gacacaagta ctttaatgaa gaggagaata ttttttcttt tcgcattgga
    30301 tgttatagct tacttatttt taggatatct tttagtgatt caaaaagcag gctatttgtt
    30361 ttcggtttta cttctttttt ctaatttttc agttccattt atcaaagaaa aggaattcga
    30421 attatttaaa aatagcaagt aatagaaaca taggttaaat cttagaacgg cttaaaatca
    30481 gatatgataa agggaaatag tcttatctag gtaactaagt gctacaaaca caatcgagga
    30541 tcacttgtta ggattacatt ttaatcatgc ttaataagaa tggagaattc ctatggggat
    30601 tgtattactc tgaagtttcg aataccttag ttctcgtgat tttgagctat tctaggttta
    30661 gaatccttca gaatgtaaaa ttttaagcag tcaaagactt agaatagccc gaaaacattt
    30721 attttatagc aattctaggc ttagaattgc tcatatatat gtatttgagc tgctcgatag
    30781 ttaggatagc tctattctga gaaatctgag ctatcaatgc ttcagaatag tattcgccaa
    30841 tggtttttat tgaagaggtg attgttttat gatagtctaa aataaaactg atttaataga
    30901 gtaaatggag aatcgttaca tgaaacattt ttttgcagga attggtgaga taaaacttgt
    30961 tggctactta ttgtatatat gtgtaggact agctcctctt tttcatatat atgtcatagg
    31021 ttcggaactg agttttgtga aaatagtgtt atctattttg ggagttatat ttgtaagtat
    31081 gttaactatt gctcgaattt atcggacttt tctataaaga atagtttgga cttgaactgt
    31141 atctaaaaac agaggttggt cgattatcag cctctttctt gtcgttgaaa agagtgagat
    31201 atattgactt ttgaaaaaag atgtcataat tgagttggga taaatctaaa aataatatag
    31261 tagattgaat ctggaatagt ccgttatgac tgttaaaata tttctagaaa ttaatttgac
    31321 tttcctaatc gatttgttca tatcttgttt caatctacta tactttctcg ttaaaccgtt
    31381 cggcaattca tggtgacatg gacacatagt agtaagcaca ctatgtggct tggcagatct
    31441 aaaaactgtt cccttgcgat aagcctaata agcacaacat agggagttag agaagccgac
    31501 tctaatcaac cactttgggc agtagtgaga actgccccgt gctttttatt ttgagaaaat
    31561 atggagtttg tcgttgaaat tacttgattg tattttggat tatcaagaaa gattcaatgg
    31621 aaaaacatgt caagtatcaa cgaattataa gtatttagag actttcaaag tgaatttttg
    31681 cttgactgat ttacatcatt tatttgactt acacaaaatc acacgagatt atgctggtca
    31741 aacaaaacct gctattcaag ctggtgtttt tattttagaa gattttagaa atatcctatg
    31801 tacaatgatg taatcgaaag gatatcttta tgtgagttta tcggcgatat cttctatatc
    31861 taagctagca agttgttgca tcgtagctaa ggatttatct aaaaatacta tgaaattgga
    31921 cgtcatattt tttgaggata aaaataaaag atccgcagtt ttaggtttac gaagagacaa
    31981 aagcggagta tttaaaccag ttactctaca ttttacaagc gctaagaaat atgctaaagt
    32041 tcgtaaaaca gatgtgaaag aaatgaaatg gctataaaga ttgtccgcag gcttaagctt
    32101 gcgttttttg ttgtttaacg aaattaaaaa acaagctctc tcatcggaaa tgtttttata
    32161 ggaatgaagg aagatgatgc tatgtttaaa aagagaatcg aaaagggcaa aagtagtgtc
    32221 tttatctttt tggagtagaa gcttatgata tagaaatttg taataaaaat gtaatataaa
    32281 acatttgaca aatatttacg gggggagtat acttaatata agtatagtct aaaaatgact
    32341 atcagaaaag aggtaaattt agatgaataa gaaaaaaatg attttaacaa gtctagccag
    32401 cgtcgctatc ttaggggctg gttttgttgc gtctcagcct actgttgtaa gagcagaaga
    32461 agcccccgta gctagtcagt ctaaagctga aaaagactat gatgcagcga agagagacgc
    32521 tgagaatgcg aaaaaagctt tagaagaagc aaaacgtgcg cagaaaaaat atgaggatga
    32581 tcagaagaaa actgaggaga aagcgaaaaa agagaaagaa gcttctaaag aggaacaagc
    32641 tgcaaatctg aaatatcaac aagagttggt taaatatgct agtgaaaaag attcagtaaa
    32701 aaaagctaaa attctgaaag aagtggagga agctgagaaa gagcataaga aaaaacgagc
    32761 agaatttgag aaagttagat cagaggtaat tcctagcgcg gaagagttaa aaaagactag
    32821 acaaaaagca gaagaggcta aagcaaaaga agcagaactt attaaaaaag tagaagaagc
    32881 tgagaaaaaa gttactgaag ccaaacaaaa attggatgct gaacgtgcta aagaagttgc
    32941 tcttcaagcc aaaatcgctg agttggaaaa tgaagtttat agactagaaa cagaactcaa
    33001 agggattgat gaatctgact cagaagatta tgttaaagaa ggtctccgtg ctcctcttca
    33061 atctgaattg gatgccaaac gaactaaact atcaacactt gaagagttga gtgataagat
    33121 tgatgagtta gacgctgaaa ttgcaaaact tgaaaaaaat gtagaatatt tcaaaaaaac
    33181 cgatgctgag caaactgaac aataccttgc tgcagctgaa aaagacttag ctgataaaaa
    33241 agctgaattg gagaaaactg aagctgacct taagaaagca gttaatgagc cagaaaaacc
    33301 agctgaagaa actccagctc cagcaccaaa accagagcaa ccagctccag caccaaaacc
    33361 agaaaaaaca gatgatcaac aagctgaaga agactatgct cgtagatcag aagaagaata
    33421 taaccgcttg ccccaacagc aaccgccaaa agcagaaaaa ccagctccag caccaaaacc
    33481 agagcaacca gttcctgcac caaaaacagg ctggaaacaa gaaaacggta tgtggtactt
    33541 ctacaatact gatggttcaa tggcgacagg atggctccaa aacaa
//
LOCUS       DAVBNO010000020        30962 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVBNO010000020 DAVBNO010000000
VERSION     DAVBNO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30962
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.020"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            289..2790
                     /locus_tag="VQ846_001036"
     CDS             289..2790
                     /locus_tag="VQ846_001036"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET3075621.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVACLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEIIAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKVEIDGKEIVKVISVPNKLVNIVVK"
     gene            2984..3679
                     /locus_tag="VQ846_001037"
     CDS             2984..3679
                     /locus_tag="VQ846_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3075622.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            3691..4107
                     /locus_tag="VQ846_001038"
     CDS             3691..4107
                     /locus_tag="VQ846_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3075623.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(4922..5014)
                     /locus_tag="VQ846_001039"
     CDS             complement(4922..5014)
                     /locus_tag="VQ846_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET3075624.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            5175..6146
                     /gene="ruvB"
                     /locus_tag="VQ846_001040"
     CDS             5175..6146
                     /gene="ruvB"
                     /locus_tag="VQ846_001040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET3075625.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            6127..6699
                     /locus_tag="VQ846_001041"
     CDS             6127..6699
                     /locus_tag="VQ846_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET3075626.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            6930..7688
                     /locus_tag="VQ846_001042"
     CDS             6930..7688
                     /locus_tag="VQ846_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET3075627.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            7697..8500
                     /locus_tag="VQ846_001043"
     CDS             7697..8500
                     /locus_tag="VQ846_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET3075628.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            8522..9781
                     /gene="rseP"
                     /locus_tag="VQ846_001044"
     CDS             8522..9781
                     /gene="rseP"
                     /locus_tag="VQ846_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET3075629.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            9794..11647
                     /locus_tag="VQ846_001045"
     CDS             9794..11647
                     /locus_tag="VQ846_001045"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET3075630.1"
                     /translation="MKQSKIPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTSEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            11746..13125
                     /gene="bglA"
                     /locus_tag="VQ846_001046"
     CDS             11746..13125
                     /gene="bglA"
                     /locus_tag="VQ846_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET3075631.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            13318..15126
                     /gene="glmS"
                     /locus_tag="VQ846_001047"
     CDS             13318..15126
                     /gene="glmS"
                     /locus_tag="VQ846_001047"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET3075632.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(15264..15341)
                     /locus_tag="VQ846_001048"
                     /pseudo
     CDS             complement(15264..15341)
                     /locus_tag="VQ846_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            15398..16447
                     /locus_tag="VQ846_001049"
     CDS             15398..16447
                     /locus_tag="VQ846_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET3075633.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(16547..20317)
                     /gene="spuA"
                     /locus_tag="VQ846_001050"
     CDS             complement(16547..20317)
                     /gene="spuA"
                     /locus_tag="VQ846_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET3075634.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSVKEAVQNESV
                     ENSSKKNIPVTPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNKKEN"
     gene            20734..21147
                     /gene="rpsL"
                     /locus_tag="VQ846_001051"
     CDS             20734..21147
                     /gene="rpsL"
                     /locus_tag="VQ846_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET3075635.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            21167..21637
                     /gene="rpsG"
                     /locus_tag="VQ846_001052"
     CDS             21167..21637
                     /gene="rpsG"
                     /locus_tag="VQ846_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET3075636.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            22062..24143
                     /gene="fusA"
                     /locus_tag="VQ846_001053"
     CDS             22062..24143
                     /gene="fusA"
                     /locus_tag="VQ846_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET3075637.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLVEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            24247..28638
                     /locus_tag="VQ846_001054"
     CDS             24247..28638
                     /locus_tag="VQ846_001054"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET3075638.1"
                     /translation="MSNSFEILMNQLGIPVEMRQHPALTQADIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKVLSQDFSNQLLQAYYREAFSEGP
                     CASQSFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEDAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPAFDFQTKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDVQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVERDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGYPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDAVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKNLIEIGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPNKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            28740..29003
                     /locus_tag="VQ846_001055"
     CDS             28740..29003
                     /locus_tag="VQ846_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET3075639.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            28996..29274
                     /locus_tag="VQ846_001056"
     CDS             28996..29274
                     /locus_tag="VQ846_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET3075640.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            29295..29447
                     /locus_tag="VQ846_001057"
     CDS             29295..29447
                     /locus_tag="VQ846_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075641.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALREK"
     gene            29469..30710
                     /locus_tag="VQ846_001058"
     CDS             29469..30710
                     /locus_tag="VQ846_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET3075642.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            30720..30950
                     /locus_tag="VQ846_001059"
     CDS             30720..30950
                     /locus_tag="VQ846_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET3075643.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
BASE COUNT         8792 a         5924 c         7248 g         8998 t
ORIGIN      
        1 gttcttcata aatcaaaaag cgacataggt agtcggctat ttattgtgaa tacattaatt
       61 agcattccag ttttatcttc ggtctaaaat aagtattttg tgctatacga gataagcttc
      121 ttgacttact ccttgattta ctgcataaca atgggataaa aagtgggaga tagagcaatt
      181 catagtcatc aaaattaatg agatacagta tacagttttt cctttaaaca catttcaaat
      241 tccctcaaaa atggtataat agtaacatca caaaattgga gagagaccat gagtttttac
      301 aatcataaag aaattgagcc taagtggcag ggctactggg cagaacatca tacatttaag
      361 acaggaacag atgcatcaaa acctaagttt tatgcgcttg atatgttccc ttatccgtct
      421 ggagctggtc tgcacgtagg acacccagaa ggttatactg caaccgatat cctcagtcgt
      481 tacaaacgtg cgcaaggcta caatgtcctt cacccaatgg gttgggatgc ttttggtttg
      541 cctgcagagc aatacgctat ggatactggt aatgacccag cagaatttac agcggaaaac
      601 attgccaact tcaaacgtca aatcaatgcg cttggatttt cttatgactg ggatcgtgaa
      661 gtcaacacaa cagatccaaa ctactacaag tggactcaat ggattttcac caagctttac
      721 gaaaaaggct tggcctatga agctgaagtg ccagtaaact gggttgagga attgggaact
      781 gccattgcca atgaagaagt gcttcctgac ggaacttctg aacgtggagg ctatccagtt
      841 gtccgcaaac caatgcgcca atggatgctc aaaatcacgg cttacgcaga gcgcttgctc
      901 aatgacttag atgaactaga ttggtcagag tctatcaagg atatgcaacg caactggatt
      961 ggtaaatcaa ctggtgccaa tgtaactttc aaagtaaaag gaacagacaa ggaatttaca
     1021 gtctttacta ctcgtccgga cacacttttc ggtgcgactt tcactgtttt ggctcctgaa
     1081 catgaattag tagatgccat cacaagcaca gagcaagcag aagcagtagc agactacaaa
     1141 caccaagcca gccttaagtc agacttggct cgtaccgacc ttgccaaaga aaaaactggt
     1201 gtatggactg gtgcttatgc catcaatcct gtcaatggta aggaaattcc aatctggatt
     1261 gcggattacg ttcttgctag ctatggtaca ggtgcggtaa tggctgtgcc tgcccacgat
     1321 caacgtgact gggaatttgc caaacaattt gaccttccaa tcgtcgaagt acttgaaggt
     1381 ggaaatgttg aagaagctgc ctacacagaa gatggccttc acgttaattc agacttccta
     1441 gatggactca acaaagaaga cgctattgcc aagattgtgg cttgcttgga agaaaaaggc
     1501 tgtggacaag agaaggtaac ctaccgtctc cgcgactggc tctttagccg tcaacgttac
     1561 tggggtgagc caatcccaat cattcattgg gaagatggaa cttcaacagc tgtccctgaa
     1621 agtgaattgc cacttgtctt gcctgtaacc aaggatatcc gtccctcagg tactggtgaa
     1681 agtccactag ctaacttgac agactggctt gaagtgactc gtgaagatgg tgtcaaaggt
     1741 cgtcgtgaaa caaatactat gccacaatgg gcaggttcaa gctggtacta cctccgctat
     1801 attgacccac acaatactga gaaattggct gacgaggatc tgctcaaaca atggttgcca
     1861 gtagatatct acgtgggtgg tgcggaacat gctgtacttc acttgcttta cgctcgtttc
     1921 tggcataaat tcctctatga cctcggtgtt gttccaacta aggaaccatt ccaaaaactc
     1981 tttaaccaag ggatgatttt gggaacaagc tatcgtgacc accgtggggc tcttgtggcg
     2041 actgacaagg ttgaaaaacg tgatggttct ttcttccatg tggaaacagg ggaagagttg
     2101 gagcaagcgc cagccaagat gtctaaatcg ctcaagaacg ttgtcaaccc agacgatgtg
     2161 gtggaacaat acggtgccga tacccttcgt gtttatgaaa tgttcatggg cccacttgat
     2221 gcttcaatcg cttggtcaga agaaggtctg gaaggaagtc gtaaattcct tgaccgtgtt
     2281 tatcgtttga ttacaagtaa ggaaattatt gcggaaaaca atggtgctct tgacaaggtt
     2341 tataacgaaa ccgttaaagc tgtcacagag caaatcgaat cattgaaatt caacacagct
     2401 attgcccaac ttatggtctt tgtcaacgcc gctaacaagg aagacaagct ctatgtggac
     2461 tatgccaaag gatttatcca attgattgcg ccatttgcac ctcacttggc agaagaactt
     2521 tggcaaactg ttgcagcaac aggtgaatca atctcttacg tagcttggcc aacatgggat
     2581 gaaagtaaat tggttgaaga cgaaatcgaa atcgtcgtcc aaatcaaagg taaagtccgt
     2641 gccaaactca tggttgctaa agacctgtca cgtgaagaat tgcaagaaat tgctcttgct
     2701 gatgaaaaag tcaaggtaga aattgacggt aaggaaatcg tgaaagtgat tagtgtacca
     2761 aataaattgg ttaatattgt tgtaaaataa ccaatttatt tgctgatttc caacctcaaa
     2821 cagtttccca aactgtttga gccaactaaa ctcgttaata tcgttgtgaa ataaggtagg
     2881 aatccttcag agtagaatct ggaggatttt ttgaatcttc ttatgaaagt atggtatact
     2941 atgggcaact ataaagtttg aaaagtgaaa aaaggagaaa gctatgccag taaatgaata
     3001 tggtcaaatg attggggagt caatggaagg ttatacacca ggtgaactgc cttctattga
     3061 tttcttagaa gggcgttatg ctaggataga ggctctttca gtggaaaagc atgcggagga
     3121 tttgctagct gtttatgggc ctgatacgcc tcaggagatg tggacctacc tttttcagga
     3181 gccagtagca gatatggggg agctggttag ccttttacat cagatgttgg ctcgtaagga
     3241 ccgtttttac tatgcaatca tagacaaggc gactggtaag gctttgggat ctttttctct
     3301 catgcgcatt gaccagaata accgagtaat agaagtcgga gcagtcactt tttctccaga
     3361 gctaaggggt acacggatag gaactgaagc tcagtatctc ctagctcgct atgtttttga
     3421 ggagctaaac tatcgtcgct acgagtggaa atgcgatgcc ttaaatctgc catccagacg
     3481 agctgcggag cgtttgagct ttatttatga aggaaccttc cgtcaggcag tggtttataa
     3541 gggacgtacg agagatacgg attggttgtc tatgattgat aaggactggc ctcaagtcaa
     3601 agatcgtttg gaaacatggt tgcgtcctga aaattttgat aaaaatggac agcagtacaa
     3661 gagcttgaga gaactttaag aggtgttgag atgattacta ttaaaaagca agaaattgtc
     3721 aagctagagg atgttttaca tctctatcag gctgtcggtt ggacaaatta tacccatcaa
     3781 ccagagatgc tggagcaggc cttatctcat tcattagtaa tttatctggc acttgatggt
     3841 gatgctgtgg tgggcttgat tcgtttggtt ggagatggat tctcatcagt attggttcag
     3901 gatttaatcg ttttaccaat ctatcagcgt caaggaattg gtagtgcctt gatgaaagag
     3961 gctttagagg attacaaaga tgcctatcaa gtccagctgg tgacagaaga gacagaaaga
     4021 accttgggct tctatcgttc tatgggcttt gaaatcttat ccacctataa ttgtataggg
     4081 atgacttgga tgaatcgtaa aaaataacaa aacttgtttt ttcttaagca aaatttaagg
     4141 atggtctagt attatatagt cattaaataa agacctccta actttattta ataaaatcct
     4201 aaactttttt catcacaatc tcctaatgaa gccacccaat caggtggctt ttttgcgtgg
     4261 tgaggtgctg gtgatagaaa ttttttgcaa aatagtaaaa ttttagaaaa gttaagctag
     4321 ttttaagttt cgtcttgtat catgtagtta ttaaataaag acctcctaac tttatttaat
     4381 aaaatcctaa acttttcttt ttcataataa tctcccttaa ctccacccaa tcaggtggag
     4441 ttttttagct ctatttcagg cttttgggga ctattctaaa aataattttt cgatattttt
     4501 cggtattttt cggattttgg tcggggaatt ggcggggact tttttagcga atataactaa
     4561 gaaataggtc tgtggtcgct tcagctagtt caaactcaat ttgattgtaa gtgactgtta
     4621 attgaagaac tggaactact gtaataatga ttttgcttgt ctagtagtgg cgtattaaat
     4681 gaaaagtgac atgtaagatt gtctcttcgt ttacgaaatc aaaatttcta tgaaattgat
     4741 agaatcccta acgttctctt aatcttaacc gtcttgtcgt acagcggttg aagaaaatgt
     4801 aaacctttga caaaattcgt gaactatggg ataataaaaa ggaattgagt actagttcta
     4861 tatcataggc tagaaaagac cccaagctca cgaccaaata agcttgaggt cttttttgac
     4921 actatttgtc cttgtttagc catttatcga ctatacgaag aacgacaccg accacaattg
     4981 gtccgatgat agttttgagt actaattcca tcatgggcta tctcacctcc tttcgtaggc
     5041 ggtgtagaag tgtcgtagaa tattatacca cataagtgct aaacttagga gtcacccaat
     5101 caggtggctt ttttgtttgt ggcttggttt tttgatataa tagagccatg agtagaattt
     5161 tagataatga gataatgggg gatgaggagt tagtagaacg cacgctccgt cctcagtatt
     5221 tacgtgaata tatcggacag gataaggtca aggaccagct acaaatcttt attgaagctg
     5281 ccaaaatgcg ggatgaagcg ctggatcatg tgctcttatt tgggcctcca ggtctcggga
     5341 aaacgaccat ggcctttgtt attgccaacg aactgggagt caatcttaag cagacgtcgg
     5401 gtccagtcat tgaaaaagcc ggagatctgg tagctatttt gaatgagtta gagcctgggg
     5461 atgtactttt tattgatgag atccatcgtt tgccaatgtc agtggaagag gtgctttata
     5521 gtgctatgga ggacttctac atagatatta tgattggggc tggtgagggt agtcgtagtg
     5581 ttcatttgga gttaccacct tttaccttga ttggtgcgac gactcgggct ggtatgctct
     5641 ccaatccgct acgggcacgt tttgggatta caggccatat ggagtattat gcccatgctg
     5701 acttgacaga aattgtcgag cggacggcag atatttttga gatggaaatc actcatgagg
     5761 cagcatctga gttggcccta cgtagtcgtg ggacccctcg tattgccaat cgtctcctca
     5821 agcgcgtgcg cgattttgcc cagataatgg ggaatggggt aattgatgat attattaccg
     5881 ataaggcttt gactatgctg gatgttgacc atgaaggttt ggactatgtg gatcaaaaaa
     5941 tccttcgtac catgattgag atgtacagtg gaggacctgt tggtctagga actctttctg
     6001 tgaatatcgc cgaagagcgt gagacagttg aagatatgta tgagccttac ttgattcaaa
     6061 aaggttttat catgcggaca cggtctggac gggtggcgac tgctaaggca tatgagcact
     6121 taggttatga atacagtgaa aaataagcaa gaaatcttag aggcttttag agaaaatcca
     6181 gatatgatgg ccattctgac gatcatccga gaccttggtc tgaaagactc gtggttggca
     6241 gcaggttctg tcagaaattt catttggaat cttttgtcag acaaatcccc ttttgatcat
     6301 gaaacagata tagatgtgat tttctttgat ccagattttt cttatgagga aaccttatta
     6361 ctggagaaaa agctgcgaga ggattttcct cagtaccagt gggaattgaa aaatcaggtc
     6421 tatatgcatc agcacagtcc tcacactgct tcctatacca gttctcgtga tgctatgagt
     6481 aagtatccag aacggtgtac gacagttgga ctgcgcttga atgaagaatc agattttgaa
     6541 ctctatgcac cttatggttt ggaggatatt ttgaattttc aagttcgtcc aactcctcat
     6601 ttcttagaaa atgaagaccg aatggaactc tatcaaacac gtttatccaa gaaaaattgg
     6661 caggagaaat ggaaaaattt gatttttaaa aatacttaag gaaactttaa gctagggagt
     6721 gtacactaag ttcataagtt aagaagacct taacttaaac tcctaaaact ttttcataat
     6781 aatctcccta taaaaataaa gtcgcccaat caggcggctt atttttttga aaaatgggct
     6841 tggtgcctga gaataaatag cttagtgata gaagaaaatg gggaaatatg gtataataaa
     6901 acgatagatt tttgaatagg aataagatca tgtttggatt ttttaagaaa gataaggctg
     6961 tggaagtaga ggttccgaca caggttcctg ctcatatcgg catcatcatg gatggcaatg
     7021 gccgttgggc taaaaaacgt atgcaaccgc gagtttttgg acataaggcg ggcatggaag
     7081 cattgcaaac cgtgaccaag gcagccaaca aactgggcgt caaggttatt acggtctatg
     7141 ctttttctac ggaaaactgg acccgtccag atcaggaagt caagtttatc atgaacttgc
     7201 cagtagagtt ttatgataat tatgtcccgg aactacatgc gaataatgtt aagattcaaa
     7261 tgattgggga gacagaccgc ctgcctaagc aaacctttga agctttaacc aaggctgagg
     7321 aattgactaa gaacaacaca ggattgattc ttaattttgc tcttaactat ggtggacgtg
     7381 ctgagattac acaggcgctt aagttgattt cccaggatgt tttagatgcc aaaatcaacc
     7441 caggtgacat cacagaggaa ttgattggta actatctctt tactcagcat ttgcctaagg
     7501 acttacgaga cccagacttg attatccgta ctagtggaga attacgtttg agcaatttcc
     7561 ttccatggca gggagcctat agtgagcttt attttacgga caccttatgg cctgattttg
     7621 acgaagcggc cttgcaggaa gctattcttg cctataatcg tcgtcatcgc cgatttggag
     7681 gagtttagga ggaaatatga cccaggattt acagaaaaga accttgtttg cagggattgc
     7741 cctggctatt ttcctaccaa ttttaatgat tgggggcctc ttgcttcaga tagcaatcgg
     7801 aatcatagcc atgctagcca tgcatgaact tttgaagatg agaggtctag agaccatgac
     7861 gatggagggc ctcttgaccc tctttgcaac ctttgcattg accattccct tggagaatta
     7921 cctgactttt ttgccagttg atgggaatgt ggttgcctat agtgttttga tttcaatcat
     7981 gttaggaacg accgttttta gcaagtctta tacgattgag gatgcggttt tccctcttgc
     8041 tatgagcttc tacgtgggct ttggatttaa tgctttacta gatgctcgtg ttgcaggttt
     8101 ggacaaggct ctcttagcct tgtgtatcgt ctgggcgaca gacagtggtg cctatcttgt
     8161 tgggatgaac tatgggaaac gaaagttagc accaagggta tcgcctaata aaacccttga
     8221 gggtgccttg ggtggtattt taggagcaat tttagtaacc attatcttta tgatagttga
     8281 cagtacagtt gctcttccat atggaattta caagatgtca gtctttgcta ttttctttag
     8341 cattgctgga caatttggtg atttactaga aagttcgatc aaacgtcatt ttggtgttaa
     8401 ggattctggg aaatttatcc ctggacatgg tggtgttttg gatcgtttcg atagtatgtt
     8461 gcttgtattt ccaatcatgc acttatttgg actcttttaa tcaaaagacg gaggaaacgc
     8521 tatgctcgga attttaacct ttattctggt tttcgggatt attgtagtgg tgcacgagtt
     8581 cgggcacttc tactttgcca agaaatcagg gattttagta cgtgaatttg ccatcggtat
     8641 gggacctaaa atttttgctc acattggtaa ggatggaacg gcctatacca ttcgaatctt
     8701 gcctctgggt ggctatgtcc gcatggccgg ttggggtgat gatacaactg aaatcaagac
     8761 aggaacgcct gttagtttga cacttgctga tgatggtaag gttaaacgca tcaatctctc
     8821 aggtaaaaaa ttggatcaaa cagccctccc tatgcaggtg acccagtttg attttgaaga
     8881 caagctcttt atcaaaggat tggttctgga agaagaaaaa acatttgcag tggatcacga
     8941 tgcaacggtt gtggaagcag atggtactga ggttcggatt gcacctttag atgttcaata
     9001 tcaaaatgcg actatctggg gcaaactgat taccaatttt gcaggtccta tgaacaattt
     9061 tatcttaggt gtcgttgttt tttgggtttt aatctttatg cagggtggtg tcagagatgt
     9121 tgataccaat cagttccata tcatgcccca aggtgccttg gccaaggtag gagtaccaga
     9181 aacggcacaa attaccaaga ttggctcaca tgaggttagc aactgggaaa gcttgatcca
     9241 agctgtggaa acagaaacca aagataagac ggcaccgact ttggatgtga ctatttctga
     9301 aaaggggagt gacaaacaag tcactgttac acccgaagat agtcaaggtc gttaccttct
     9361 aggtgttcaa ccgggggtta agtcagattt tctatccatg tttgtaggtg gttttacaac
     9421 tgctgctgac tcagctctcc gaattctctc agctctgaaa aatctgattt tccaaccgga
     9481 tttgaacaag ttgggtggac ctgttgctat ctttaaggca agtagtgatg ctgctaaaaa
     9541 tggaattgaa aatatcttgt acttcttggc aatgatttcc atcaatattg ggatttttaa
     9601 tcttattccg attccagcct tggatggtgg taagattgtg ctcaatatcc tagaagccat
     9661 ccgccgcaaa ccattgaaac aagaaattga aacctatgtc accttggccg gagtggtcat
     9721 catggttgtc ttgatgattg ctgtgacttg gaatgacatt atgcgactct tttttagata
     9781 atcgaggaat attatgaaac aaagtaaaat acctatccca acgcttcgcg aaatgccaag
     9841 cgatgctcaa gttatcagcc atgctcttat gttgcgtgct ggttatgttc gccaagtttc
     9901 agcaggtgtt tattcttatc taccacttgc caaccgtgtg attgaaaaag ctaaaaacat
     9961 catgcgccaa gaattcgaaa agattggtgc tgttgagatg ttggctcctg cccttcttag
    10021 tgcagaattg tggcgtgaat caggtcgtta cgaaacctat ggtgaagacc tttacaaact
    10081 aaaaaaccgt gaaaaatcag actttatctt aggtccaact cacgaagaaa cctttacagc
    10141 tattgtccgt gattctgtta aatcttacaa gcaattgcca ctcaaccttt atcaaattca
    10201 gcccaagtat cgtgatgaaa aacgcccacg taatggactt cttcgtacac gtgagtttat
    10261 catgaaggat gcttatagtt tccacgctaa ctatgatagt ttggatagtg tttatgatga
    10321 gtacaaagca gcctatgagc gtattttcac tcgtagtggt ttagacttca aggctattat
    10381 tggtgacggt ggagccatgg gtggtaagga tagccaagaa tttatggcca ttacatctgc
    10441 tcgtacagac cttgaccgct gggttgtctt ggacaagtca gttgcctcat ttgacgaaat
    10501 tcctgcagaa gtgcaagaag aaatcaaggc agaattgctc aaatggatag tctctggtga
    10561 agataccatt gcttactcaa gtgagtctag ctatgcagct aacttagaaa tggcaacaaa
    10621 cgagtacaaa ccaagcaacc gtgttgtcgc tgaagaagaa gttactcgtg ttgcaacgcc
    10681 agatgttaaa tcaattgatg aagttgcagc tttcctcaat gttccagaag aacaaacgat
    10741 taaaaccctc ttctacattg cagatggtga gcttgttgca gcccttctag ttggaaatga
    10801 ccaactcaac gaagtcaagt tgaaaaacca cttaggagca gatttctttg acgttgctag
    10861 cgaagaagaa gtggcgaatg ttgttcaagc aggatttggt tcacttggac cagttggttt
    10921 gccagagaat attaaaatta ttgcagatcg taaggtgcaa gatgttcgca atgcagttgt
    10981 cggtgctaac gaagatggct accacttgac tggtgtgaac ccaggccgtg attttacttc
    11041 agaatatgtg gatatccgtg aagttcgtga gggtgaaatt tccccagatg gacaaggtgt
    11101 ccttaatttt gcgcgtggta ttgagatcgg tcatattttc aaactcggaa ctcgctattc
    11161 agcaagcatg ggagcagatg tcttggatga aaatggtcgt gctgtgccaa tcatcatggg
    11221 atgttacggt atcggtgtca gccgtcttct ttcagcagtg atggagcaac acgctcgcct
    11281 ctttgttaac aaaacgccaa aaggtgaata ccgttacgct tggggaatca atttccctaa
    11341 agaattggca ccatttgatg tgcatttgat tactgtcaat gtcaaggatg aagaagcgca
    11401 agccttgaca gaaaaacttg aagcaagctt gatgggagct ggttatgaag tcttgacaga
    11461 tgaccgtaac gaacgtgtcg gtgttaaatt tagcgatagt gacttgattg gtcttccaat
    11521 ccgtatcact gttggtaaaa aagcggccga tggcattgta gaagttaaga tcaaggcaac
    11581 tggtgacacc attgaggttc atgcagataa cgtgcttgaa acgcttgaaa tcctaagcaa
    11641 gaaataaaaa ctataatcag aagaaaaaca aggaaaaaat gtaactagtt tttaccttgt
    11701 tttttctgta taatgggaga aatgagtaga taaagaggta aatgtatgct aagatttcca
    11761 aaggattttg tctggggatc ctctacttct ggaccgcaga cagaaggacg tgtagctggt
    11821 gacggtaagg gagacaatct ctgggattac tggttccaag tggagccaaa tcgttactat
    11881 aatgggattg gttcagataa gacatcgact ttttatgaaa attgggagcg ggatattgag
    11941 cttttgttag agactggtca cacagccttt cggacttcta ttcagtggtc acggattttt
    12001 ccacaaggct gtggaaaagt caaccctcaa ggtgtggatt tttatcgtaa ggtctttgag
    12061 gctattaagg ctaaaggaat tcgtctgtta gtcaatctct atcattttga tttacctttt
    12121 gcccttcaag aggatggtga tggttgggaa aataaggcga cagtctcagc ctatgaagac
    12181 tatgctcgtt tttgttttga gacttatgga gatttagtgg atcaatggat tacctttaac
    12241 gagcccatcg ttcctgtaga atttggttat ttttatgatg cccattatcc acataaggtg
    12301 gatgcagagg cagctgttaa ggtagcctat catacacaat tggccagcag ccgggctgtt
    12361 aaggcttgcc atgaactttt gcctgattcc aagattggga ttgtcctcaa cttgacaccg
    12421 gcttatccac gtagccagca tcctgctgat gtcaaggcag ctcgtattgc ggcccttttt
    12481 caggcccaat ctttcttaga tccatctgtc ttggggactt atccacagga gttggtagaa
    12541 atcttgcatg aacacggtct tttacctgat gctacagagg aagagttgga actcattcgt
    12601 gataatacgg tggacttcct tggtgtgaac tactatcaac ctttgcgtgt tatggctcct
    12661 cgatttgcta agcatccaga gagtccactc ttaccagaac atttttacga gccttatgtg
    12721 atgcctggac gtaaaatcaa ttctcaccgt ggttgggaga tttatgagca agggatttat
    12781 gacatcgccc aaaatatcaa ggaaaattat ggcaatattg agtggatgtt gactgagaat
    12841 ggtatgggtg ttgaagggga agaaaaattc cgtcaagatg gaatgattca agatgattac
    12901 cgtattgact ttgtaaaagg tcatcttcgt gaacttcacc gtgccattga agatggtgcc
    12961 aactgtaagg gctacttgat ttggaccttt attgactgct ggtcatggct caacagctat
    13021 aaaaatcgct atggtttggt cgaattagac ttggaaacgc aagaacgtcg tctgaagaaa
    13081 tcaggccact ggttcaaaga attaagcgat aataatggat tttaaaaagg actctgactg
    13141 attatcccaa tcggtcagag tttttatgct tacaggagct taatttgaac tataccaatt
    13201 tttactcttg acaagtgaaa gaaacaagta tatactgttt ttgctgattc tataaaagat
    13261 gaattagact ataccaattt taaggagaaa gcacagcttg cctgtgtcgt atatactatg
    13321 tgtggaattg ttggtgttgt tggaaacaca aatgcaactg atattttgat tcaagggctt
    13381 gaaaagcttg aataccgtgg ctatgattct gcgggaattt ttgtcctaga tggtgctgat
    13441 aaccatttgg tgaaggcggt tggtcgtatt gcagaattgt ctgccaagac agctggtgtt
    13501 gagggaacaa ctggtatcgg acatactcgt tgggctactc acggaaaacc aactgaggac
    13561 aatgctcacc cacaccgctc tgagacagaa cgctttgtct tggtgcataa tggggtgatt
    13621 gagaactatc ttgaaatcaa ggaagaatac cttgcaggtc accacttcaa ggggcagaca
    13681 gatactgaaa tagccgttca tttgattgga aaatttgcgg aagaagaagg gctctcagtt
    13741 cttgaagcct tcaaaaaagc ccttcacatc atccgtggtt cttatgcctt tgccttggtt
    13801 gactcacaag atcctgaagt catctacgta gctaaaaata aatcaccact tttgattggt
    13861 cttggagaag gctataacat ggtctgctca gacgccatgg ctatgattcg tgaaactaac
    13921 caatacatgg aaatccatga ccaagagttg gtaatcgtca aggctgatag tgtggaagtt
    13981 caagactatg atggaaatcg tcgtgagcgt gctagctaca ctgctgaact tgacttgtca
    14041 gatatcggta agggaactta cccttactac atgctcaagg aaattgacga gcaaccaact
    14101 gttatgcgga aactcatcca agcctacaca gatgaggctg gtcaagttgt cgtagaccca
    14161 gatatcatta aggctgttca agacgcagac cgcatctaca tccttgcagc tggaacatct
    14221 taccatgcag gatttgcttc taagaaaatg ttggaagaat tgacagatac accagttgaa
    14281 cttggaatct catctgagtg gggctacggt atgccacttc tcagcaagaa accactcttc
    14341 atctttatca gccaatctgg tgaaacagcg gatagtcgtc aagttttggt caaggctaat
    14401 gaaatgggaa ttccaagctt aacagtgaca aacgttccag gttcaaccct ctcacgtgaa
    14461 gccaactata ccatgctcct tcacgcaggt cctgaaattg ccgtggcatc aactaaagcc
    14521 tatacagcgc aaatcgcagc ccttgccttc cttgcaaaag cagtcggaga agcaaatggt
    14581 aatgctaaag cgcaagcctt tgacctggtt catgaattgt caatcgtagc tcagtctatc
    14641 gaatcaactc tttcagagaa agaaaccatt gaagccaagg ttcgtgaact tcttgaaaca
    14701 actcgtaacg ccttttacat cggacgtggt caagattact acgtagccat ggaagcaagt
    14761 ctcaaactca aagagatttc ttatatccag tgtgaaggct ttgctgcagg agaactcaag
    14821 cacggaacca ttgccttgat tgaagaagga acgcctgttt tggctctctt gtcagatcca
    14881 gttcttgcca atcatactcg tggaaatatc caagaggtcg cagcccgtgg tgccaaagtc
    14941 ctcactatcg cagaagagaa tgtagccaaa gataccgacg atatcgtcct tacgaccgta
    15001 catccatacc tctcaccaat ttcaatggtc gtaccaacgc aattagtcgc ttactttgca
    15061 accctccacc gtggcctcga tgtggacaaa ccacgtaacc ttgccaagtc agtaacggta
    15121 gaataagcta aaaaagtcta gttatctagg cctttttctt gtgagcaaat tggttgcttg
    15181 tactcaagat tcgtgttata ctcttcgaaa atctcttcaa accacgtcaa cgtcgccttg
    15241 ccgtaggtat ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca ctgttttgag
    15301 cagcctgcag ctagcttcct agtttgctct ttgattttca ttgagtatta gaataatttt
    15361 tcaaattgaa gtacagagat agataaggag aatcacaatg gtagaattgg gaatttcaac
    15421 atttggggaa ataacggagc ttgaagggac tggacaaact tacagtcatg ctgaacgcat
    15481 tcgccagttg gtggcagaga ttgagctggc tgacaaggtt ggtttggatg tatatgggat
    15541 tggtgagcac catcgggcgg attttgcagt atcagcccca gagattgttc tggccgctgg
    15601 ggcagtcaat accaagaaaa tccgtttgac cagtgcagtc agcattctgt caagcatgga
    15661 cccgattcgt ttgttccaac agtatgccac tatcgatgct ttgtcaaatg gacgagcgga
    15721 gattatggct ggaaggggct ctttcacgga atctttccct ctgtttggtt atgacttgaa
    15781 agactacgac tctctttttg atgagaaatt agacttgctt cagttagtca atgaaaagac
    15841 caagttagac tggcaaggtc gattgactca aacgattgct ggtaaagaag tttatcctcg
    15901 tccagttcag gacaaattgc ccttgtggat agctacaggt ggtcatgtcg aatcaacagt
    15961 gaagattgct caggctggtc taccgattgt atatgctatt attggtggta atccacgtta
    16021 ttttaaaaag ttgattcagg cttatcgtga gattggaagc gaagcgggtc atgctgacaa
    16081 agacttgaaa gtgggagccc attcttgggg gtggattgcg gaagatggtg agcaggcggt
    16141 gaaagattat ttccatccga ctaagcaagt ggtggatgct atttccaaag accgtccgca
    16201 ctggcaggaa ttgcgttatg agcaatattt ggagcaggta gggccaaatg gcgccatgtt
    16261 tgttggcaat ccagatcagg tggccgaaaa attgattcgc atgatagagg atttaggatt
    16321 ggaccgcttc atgcttcatc taccgcttgg ttctatgcct catgaccagg ttctgagagc
    16381 tattgaactc ttcggaacac aagttgcacc caaagtacga gcttattttg ctatgaaaga
    16441 ggcttaataa aaaatcctaa tcaagtttat taggacaaca aatattgtac gaaaattaat
    16501 cccccgagct ataatgcttg ggggattttt ctataggagg gctagtttag ttctcttttt
    16561 tattttttag taagaaaccg agaccaagga gcgcaaggag gctgattcct gcaaatagga
    16621 gtttgttttc gtttttgatt cctgtatttg gaagttcagc ttgtttatct ggggttacag
    16681 gtatattttt cttgctagag ttttctaccg attcgttttg aaccgcttct tttacagatg
    16741 atgcttgtgc ttcttgtgct ggttgttcag cttgtgaatc agctgtagct tggctaggtt
    16801 tattttccgc atcagctact ttggcatctg gtttagtaga atcaggtcta gcttctggag
    16861 ctgggtcttg atgttcaggc ttggaagggg ttgagtctgg tttctcttct gcagttgact
    16921 catggctagt tccattttga gagactcgaa gaacagtagc tgtaagggca ttcaatttca
    16981 agcctttttc agtccattca agtcctttcg ggttggcaat tccgactgat cctgcttggt
    17041 tttcatctgc caaaacttcc gcatttctta gatgtgcaaa ggcagttccc aaattaaatt
    17101 cgcgagcttt ttcatccgca ttgacaaaga ctgcgtagat atcgccgttt ggagcagtga
    17161 tttggtagcc aatcactaca tcctcttttt ccacaccatt ttggcctggg acagtgatga
    17221 ggtggacacg gtctttgata tcttggagac tcttaagtcg gaaggcatct gtagattgac
    17281 gaagggcaat caaacctttc atatagtcac ggctcttgac attttcagga taagctttac
    17341 catctgtagc cttagtccag tcaaacttgt tgactgcgtc gctagaatcg taagaatcat
    17401 ggatgaagta aggatagtca aatgggttgc cgtccttatc acgcaacaag tgagatttgt
    17461 ttggaacctt atcctctgct actggagtct tgtaggctgg gtcacggaat tgtttagtac
    17521 gtccatattc ctgaccggag tggataaatg gagttccttg agctgtcaag accatgagat
    17581 ttccaagtcg taaacgacgg tggatttcag catagttctc agccttgctt gggtcttttt
    17641 tgatagactg ggcaatgatg tcaaagaggg tcaagttatc atgggctgcg atgtattgga
    17701 tgacatctcc agggctgtca gcttcaaagt tagttggctg agcaatgaga tttttaaaga
    17761 tagtgttaac atcacgcttg ccacctgtga taaaggcagg ttgaccttcg tttggataac
    17821 cagatttgag gttgttacgg atgtcatctg aaaagacagc gacagtatcg gtatgtttca
    17881 tccaatcttg gtcagcagct ttagtaggca tgttttcatc accggcatag gttctccaac
    17941 cttcgcctaa catgattaga tttggattga gggcgcgtgc agccttgtaa gcttcttcga
    18001 tagaagcggc gtcatggtct cccatcatat cgaaacggaa tccatccact ttgtaggtat
    18061 caactaggta tttgatagag tcaactagga ggcgtttggt catatggtgg gttgtcccca
    18121 agcgtccacc accaaagcta gttcgaggtg tgccatcggc atccataaag tggtagtagt
    18181 ttggctctag gtcttcaaag atatcgactt tggctgtgtg gttataaacg acatctagga
    18241 tagctcccat accacgtttg tggatttcgt tgatgaggtt tttaaattct gcgattcgtt
    18301 tttctggatt cttaggatcg cttgagtaca taccagtcaa ggagaagtag ttttgagggt
    18361 catatcccca gttgtagttg ctgttgcttg aagcatagtc agacaagcgt tcatggtttt
    18421 tcaattcatt gacaaagtag taagacaaga ctggaaggag ctggatatgg gttacaccca
    18481 agtctttgag atagtctagt ttttcaatga aggcttcaaa agtcccaaat ggtttggtca
    18541 agtcttttgc aatggcagga tctgaagtga agtcacgcac atgagcttcg tagataacgg
    18601 cgtcttcacg agtcttgaaa ttgtgaatct taccataagt caagtcttga ggtccgagtt
    18661 tagctggatc tacaaaggcg gctttagcca ctttatgggc atcgtcaatc ttggcatcgt
    18721 cgctattcca agcagcaaga gatttagcgt aaggatcgag tgcaagaaca gttttacctt
    18781 gacgctcgat ttggtattga taatagtagc cagtgaaatc tgtgattccg agtttgtttg
    18841 tgctgtctag agtttgtttc caagttcctc tttccccttt ttcgagagcg acagttccaa
    18901 ctactttgtc agggtcattc ttgtcgtaga caacaacaga aaccttatca gcacttggtg
    18961 accaaagggt caaatcaact tgttttcctt cttcttttag gtcagctccc agtttgccat
    19021 catagctgta tgtctcatct ttcaggcgcc agcttgtttt ggtagtgaat tggtcggaat
    19081 tgtagctaac agtataagga tgttttgtgt cagagaaatc tccgctgtag gtcactttct
    19141 taccagcttc atcgattgca acatcggtaa tagttacctt gtttcctagg tgattagtga
    19201 tgttggagtg tttgaggata tcttcttttt tagcaccgac aagtgttgaa aagctacttt
    19261 caatgctaga agtgcctacg tgttgggctc ctgtcatacg gatatcatgg acatagtatg
    19321 gatttgtgta aatcgattca tcatcgtctt ttaggaaaat ttggctatga tttttcaaat
    19381 ctgtgaactt ataattttct ttacggattt tcacgtcgtc tccttgtttg ctctcatcta
    19441 gtaataaaaa tccaaattct cttgcggctt cattaagagg aatgtcgata tagcggccat
    19501 atttgcctgt agccgtaaag tctgttccgt caggccattg agcgctactt ggatttttta
    19561 catctcccca gtaccagaga gatttcttgt catagttgcc atctgtgcgg tagtagttga
    19621 cgcgaacagt tcctgcaggc tgtggctcgt aagagaaaac cttgtaatct tggtctaacc
    19681 aagcttcgtt catttttgga actagttttt ctacagattt atcgccggtt agattttttc
    19741 cagctgtatt gttgatgagg aagctaattt tcttggcttg ttctcccttt aatttgacat
    19801 ctaggtaata gccgtagtca tctttcttgg catccttgaa ggacaaagct ccgtttggcc
    19861 agttttcaga tggtttttca acatcgtccc aagtccatag tccttgagca tccttgtttt
    19921 cttcaggaag ttttttgaca tggatacgga agtagttatc ttcgatgggc tcttctgcct
    19981 tagtttcagt tgttactgga ctagtagaag tagttggctc tgttgcaggt ttttcttctg
    20041 ttttctctag ggaagcgttt gcatttctag gtgaagaaat atcactttcg ttcttaccag
    20101 agccagctgc tgtatcagta agaggttggg caagggtggt tgtatttctg ttgttgggtt
    20161 cgttagtggt tggtgtactt tcgtcggatg agatagttgg tgtagccatg gcaagcaaga
    20221 caaggcttgc gccgataagg acagaaccag ttccattttt gagggaacgg atgctgtaga
    20281 ccattttttt ctcagtgtga gatggtgttt ttctcataat gattctccaa tcaataaatt
    20341 tctatatcag tatagcatgt agtaaaacaa tatgcaagcg ttttctttga tttgtgaaaa
    20401 aaattcctac ttttgagaag ttttctgtac tattttatat atcagtataa atgacggaga
    20461 taaaaatgtc acataagcca agccgtatta ttaggaagat gatgtgttcc cagctatttt
    20521 tattcagaaa ttgatctcta acattaccaa taaaaatgaa aaatatcttg acaagcaagg
    20581 gaaaattttg ttacaataat agacggtact ttttactttt ggtctctcaa gagtgtacag
    20641 ggacgtgctg acaaatgttg caaaagtaca cacagatgat atctgtcacc aagtgtatca
    20701 tcaccaaaaa taaaaaaaca caggagaatg tagatgccta caattaacca attggttcgc
    20761 aaaccgcgta aatcaaaagt agaaaaatct aaatcaccag ctttgaacgt tggttacaat
    20821 agtcataaaa aagttcaaac aaacgtttct tcaccacaaa aacgtggtgt tgcaactcgt
    20881 gttggaacaa tgacacctaa aaaacctaac tcagcccttc gtaaattcgc tcgtgtacgt
    20941 ttgagcaacc ttatcgaagt tactgcctac atcccaggta tcggacacaa cttgcaagag
    21001 cacagcgtgg tgcttcttcg cggtggacgt gtaaaagacc ttccaggggt acgttaccat
    21061 atcgtccgtg gtgcacttga tactgcaggt gttaacgatc gtaaacaagg ccgttctaaa
    21121 tacggtacta aacgtccaaa agcataagga aaggggataa agagaaatga gtcgtaaaaa
    21181 tagagctcca aaacgtgacg tattgccaga tccgctatac aattcacaac tagttactcg
    21241 tcttatcaac cgcgttatgc ttgatggtaa acgtggtact gctgcttcaa tcgtttacgg
    21301 tgcttttgag caaatcaaag aagctactgg caacgatgca cttgaagtat ttgaaacagc
    21361 tatggaaaac atcatgcctg tacttgaagt acgtgcacgt cgtgttggtg gttctaacta
    21421 ccaagtccca gttgaagttc gtccagaacg tcgtacaaca cttggacttc gttggttggt
    21481 aacaatcgct cgtcttcgtg gtgaacacac aatgcaagac cgtcttgcaa aagaaatctt
    21541 ggatgctgct aacaacactg gtgcagcagt taagaaacgt gaagatactc accgtatggc
    21601 tgaagctaac cgtgcattcg cacacttccg ttggtaagat aggatgcgaa agcgttaaga
    21661 aagtcccaga gaaaataggg aatcgaagca ggttgcggtt gcaaccaatg agattcatct
    21721 ttttctccag acttttagct tgagctcaac taaatcatga tgctaggcac ggtaaggatg
    21781 caaggtgaaa ataggaaact gacgcagtat tcaacgaata caaggagttt tatctttttc
    21841 acgcagcatc ccgttccagc tcacatcggc taactaactt tagcctgggt tcaaattagc
    21901 taaatcgatt agtattagct ataactcagc ttaccatctc gtaagttgaa accaacaata
    21961 gcatgaaaac attgagaacg ggtaggtcct gcctatccgt ttttattaaa atcgtgttat
    22021 aatagaatag aaatcaaaaa taaataggag aaacaaacct catggcacgc gaattttcac
    22081 ttgaaaaaac tcgtaatatc ggtatcatgg ctcacgtcga tgccggtaaa acaacaacta
    22141 ctgagcgtat tctttactac actggtaaaa tccacaaaat cggtgaaact cacgaaggtg
    22201 cgtcacaaat ggactggatg gagcaagagc aagaacgtgg tatcacgatc acatctgctg
    22261 cgacaacagc tcaatggaac aaccaccgcg taaacatcat cgacacacca ggacacgtgg
    22321 acttcacaat cgaagtacaa cgttctcttc gtgtattgga tggtgcggtt accgttcttg
    22381 actcacaatc aggtgttgaa cctcaaactg aaacagtttg gcgtcaagca actgagtacg
    22441 gagttccacg tatcgtattt gccaacaaaa tggacaaaat cggtgctgac ttcctttact
    22501 ctgtaagcac acttcacgat cgccttcaag caaatgcaca cccaatccaa ttgccaatcg
    22561 gttctgaaga tgacttccgt ggtatcattg acttgatcaa gatgaaagct gaaatctata
    22621 ctaacgacct tggtacggat atccttgaag aagacatccc agctgaatac cttgaccaag
    22681 ctcaagaata ccgtgaaaaa ttggttgaag cagttgctga aactgacgaa gaattgatga
    22741 tgaaatacct cgaaggtgaa gaaattacta acgaagaatt gaaagctggt atccgtaaag
    22801 cgactattaa cgttgaattc ttcccagtat tgtgtggttc agccttcaag aacaaaggtg
    22861 ttcaattgat gcttgatgcg gttattgact accttccaag cccacttgac atcccagcaa
    22921 tcaaaggtat taacccagat acagatgctg aagaaactcg tccagcatct gacgaagagc
    22981 catttgcagc tcttgccttc aagatcatga ctgacccatt cgtaggtcgt ttgacattct
    23041 tccgtgttta ctcaggtgtt cttcaatcag gttcatacgt attgaacact tctaaaggta
    23101 aacgtgaacg tatcggacgt attcttcaaa tgcacgctaa cagccgtcaa gaaattgaaa
    23161 ctgtttactc aggtgatatc gctgctgccg ttggtttgaa agatactaca actggtgact
    23221 cattgacaga tgaaaaagct aaaatcatcc ttgagtcaat caatgttcca gaaccagtta
    23281 tccaattgat ggttgagcca aaatctaaag ctgaccaaga caagatgggt atcgcccttc
    23341 aaaaattggc tgaagaagat ccaacattcc gcgttgaaac aaacgttgaa actggtgaaa
    23401 cagttatctc tggtatgggt gagcttcacc ttgacgtcct tgttgaccgt atgcgtcgtg
    23461 agttcaaagt tgaagcgaac gtgggtgctc ctcaagtatc ttaccgtgaa acattccgcg
    23521 cttctactca agcacgtgga ttcttcaaac gtcagtctgg tggtaaaggt caattcggtg
    23581 atgtatggat tgaatttact ccaaacgaag aaggtaaagg attcgaattt gaaaacgcaa
    23641 tcgtcggtgg tgtggttcct cgtgaattta tcccagcagt tgaaaaaggt ttggtagaat
    23701 ctatggctaa cggtgttctt gcaggttacc caatggttga cgttaaagct aaactttacg
    23761 atggttcata ccacgatgtc gactcatctg aaactgcctt caagattgcg gcttcacttg
    23821 ctcttaaaga agctgctaaa tcagcacaac cagcgatcct tgagccaatg atgcttgtaa
    23881 caatcactgt tccagaagaa aaccttggtg atgttatggg tcacgtaact gctcgtcgtg
    23941 gacgtgtaga tggtatggaa gcacatggta acagccaaat cgttcgtgct tacgttccac
    24001 tcgctgaaat gttcggttac gcaacagttc ttcgttctgc atctcaagga cgtggtacat
    24061 tcatgatggt atttgaccac tacgaagatg tacctaagtc agtacaagaa gaaatcatta
    24121 agaaaaacaa aggtgaagac taatcagtcc tcactctaga aggaagtcac ttagtggctt
    24181 ccttttgtct ttagaaaata cctctaaata tggtaaaata gtagaagaat aatgtgagga
    24241 aaatgaatgt caaatagttt tgaaattttg atgaatcaat tggggatacc tgttgaaatg
    24301 agacagcatc ctgctttaac acaggcagat attgagcgag tcgtggttca taaaattagt
    24361 aaggtatggg agtttcattt cgtattttct aatattttac cgattgaaat ctttttagaa
    24421 ttaaagaaag ggctgagtga ggaattttct aagacaggca ataaagctgt tttcgaaatc
    24481 aaggttctgt ctcaagattt ttcaaatcaa ctcttgcagg cttattatag agaagctttt
    24541 tctgagggtc catgtgctag tcaaagtttt aagtctcttt atcaaaattt gcaagttcgt
    24601 gctgagggga atcaactctt tattgaagga tctgaggcga ttgataagga acactttaag
    24661 aaaaatcacc ttcctaattt agcaaaacaa cttgaaaagt ttggttttcc aacttttaac
    24721 tgtcaggtcg agaagaatga tgtgctgacc caagagcagg aagatgcctt ccatgctgaa
    24781 aatgagcaga ttgttcaagc ggccaatgag gaagcgctcc gtgctatgga acaactagaa
    24841 cagatggcgc cacctccagc ggaagagaaa ccagcctttg attttcaaac gaaaaaagct
    24901 gcagctaaac ccaagctaga taaggcggag attactccta tgatcgaagt gacgacggag
    24961 gaaaatcgtc tggtctttga aggggttgtg tttgatgtgg agcaaaaagt gaccagaaca
    25021 gggcgtgttt tgatcaactt taaaatgacg gactacactt caagtttttc gatgcaaaag
    25081 tgggtaaaaa atgaggaaga ggctcagaaa tttgatctca tcaagaagaa ttcttggctc
    25141 cgagttcgtg ggaatgtgga gatgaataac ttcacacgcg atttgactat gaatgtgcag
    25201 gatgtgcagg aagttgttca ctatgagcgg aaggatttga tgccagaagg tgagcgtcgg
    25261 gttgagtttc acgctcatac taacatgtcg actatggatg ctctaccaga ggttgaagaa
    25321 atcgttgcta cagctgctaa gtggggacac aaagcagttg ctatcaccga ccacgggaat
    25381 gttcaatcct tcccacacgg ctataaggcg gctaagaaag cgggaatcca gctaatctat
    25441 gggatggaag ccaatatcgt ggaggaccgt gtccctatcg tctataacga agtggagagg
    25501 gacttgtcag aagcgaccta cgtggtcttt gacgtggaaa cgacgggtct atcagccatc
    25561 tataatgatt tgattcaggt tgcggcctct aagatgtaca aggggaatgt cattgctgaa
    25621 tttgatgaat ttatcaatcc tgggtatccc ttgtcagctt ttactactga gttgactgga
    25681 attacagatg atcacgtcaa aaatgccaaa ccactagaac aagttctgca agaattccaa
    25741 gaattctgca aggatacggt cctagttgcc cacaatgcga cctttgacgt tggctttatg
    25801 aatgccaact atgagcgtca tggtcttcct aaaattagcc agccagttat tgatacgctg
    25861 gagtttgcta gaaacctcta tcctgagtat aaacgtcatg gtttggggcc tttgactaag
    25921 cgttttggtg tggctttgga acatcaccac atggccaatt acgatgcgga agctactggt
    25981 cgtctgctct ttatttttat caaagaagta gcagaaaaac atggagtgac cgatctagct
    26041 agactcaaca ttgatttgat tagtccagat tcttataaaa aagctcggat caagcatgcg
    26101 actatttatg tcaagaatca ggtaggtcta aaaaatatct ttaagctagt ttctttgtcc
    26161 aatactaagt actttgaagg ggtgccacga attccgagaa cagttctaga tgcccatcgg
    26221 gaaggtttga ttttaggttc agcttgctct gaaggtgaag tttttgatgc ggttgtttcc
    26281 caaggtgtgg atgcggcggt tgaggtggcc aagtattatg actttatcga ggtcatgcca
    26341 ccagctatct atgctccctt gattgccaag gagcaggtca aggatatgga agaactccag
    26401 actattatca agaatttgat agagatcggc gaccgtcttg gcaagcccgt tttggctacg
    26461 ggaaatgtcc actatatcga accagaagaa gaaatttacc gtgaaattat cgtccgcagt
    26521 ttgggtcagg gggcgatgat taaccgaacc atcggtcatg gtgaacatgc ccaaccagca
    26581 ccacttccaa aggctcattt tagaacgact aatgagatgt tggatgaatt tgccttcttg
    26641 ggagaggaac tagcccggaa attggttatt gaaaacacca atgccttggc agaaatcttt
    26701 gaacccgttg aggtagttaa gggtgacttg tatacgcctt tcattgacaa ggctgaagaa
    26761 acagttgctg agttgaccta taagaaagct tttgagattt atggaaatcc gttgccagat
    26821 attgttgatt tgcgaattga aaaagaattg acttccattc tggggaatgg atttgctgtg
    26881 atttatctgg catcgcagat gctggttcaa cgttccaatg aacggggtta cctggtgggt
    26941 tctcgtgggt ctgtaggatc tagtttcgtt gcgactatga ttggtattac ggaggttaat
    27001 cctctctctc ctcactatgt ctgtggtcag tgtcaataca gcgagtttat caccgatggt
    27061 tcttatggtt caggttttga tatgcccaat aaggactgtc caaactgcgg tcacaaactc
    27121 agtaaaaacg gacaggatat tccgtttgag accttccttg gctttgatgg ggataaggtt
    27181 cctgatattg acttgaactt ctcgggagaa gatcagccta gcgcccactt ggatgtgcgt
    27241 gatatctttg gtgaagaata tgccttccgt gcgggaacgg ttggtacggt agctgccaag
    27301 actgcctatg gatttgtcaa gggttacgag cgagattatg gcaagtttta tcgtgatgca
    27361 gaagtagaac gcctcgctca aggagcggcg ggtgtcaagc ggacaacagg ccaacacccg
    27421 gggggaatcg ttgttattcc gaactacatg gatgtctacg attttacgcc tgtccagtat
    27481 ccagcagatg atgtcacggc tgaatggcag accactcact ttaacttcca cgatatcgat
    27541 gagaacgtcc tcaaactcga tgtactggga catgatgatc cgaccatgat tcgaaaactt
    27601 caggatttgt ctggtattga ccctaatgaa attcctatgg atgacgaagg cgtgatggca
    27661 ctcttttctg ggactgatgt gctaggggta acacctgaac aaattggaac gcctacgggt
    27721 atgttgggga ttccagagtt tggaacaaat ttcgtacgtg gaatggtaga cgaaacccat
    27781 ccgacaacct ttgcggaatt gcttcagctg tctggtctgt cccacggtac tgacgtttgg
    27841 ttggggaatg ctcaggatct gattaagcaa ggaatagcgg acctatcgac tgttatcggt
    27901 tgtcgggacg acatcatggt ttacctcatg catgcgggtc tggaacctaa gatggccttt
    27961 accattatgg aacgggtacg taagggtttg tggctaaaga tttcagaaga ggagagaaat
    28021 gactatatcg aagccatgaa ggccaataag gtgccagagt ggtatatcga atcctgtggg
    28081 aaaattaagt acatgttccc taaggcccat gcggcagcct acgttatgat ggccttgcgt
    28141 gtagcttact tcaaggttca ccatcctatt tattactact gtgcttactt ctccattcgt
    28201 gctaaggctt ttgatatcaa gaccatgggt gcgggcttgg aggtcatcaa gcgcagaatg
    28261 gaagaaatct ctgaaaaacg gaagaacaat gaagcctcta atgtggaaat cgatctctat
    28321 acaactcttg agattgtcaa tgagatgtgg gaacgaggtt tcaagtttgg caaattagat
    28381 ctctaccgta gtcaggcgac agagttcctc atcgacgggg atacccttat cccaccattt
    28441 gtagcaatgg atggtctggg agagaacgtt gccaagcaac tggtgcgggc gcgtgaagag
    28501 ggagaattcc tctctaaaac agaactacgc aagcgtggtg gactctcatc aaccttggtt
    28561 gaaaagatgg atgagatggg tattcttgga aatatgccag aggataacca gttgagtttg
    28621 tttgatgagt tgttttaaaa aattgcttaa taatctatta aaagaggcta acgtatatcc
    28681 aatagattta cattagcttt cttttttgtt aaaatagtct atggaaagag ggtgagagta
    28741 tgtcaaagat gagtataagc atccgtctgg atagtgaggt taaggagcag gcccaacagg
    28801 tgtttagtaa tctgggaatg gatatgacaa cagctattaa tattttcctt cgtcaggcaa
    28861 ttcaatatca gggattacct tttgatgtta gactagacga aaatcggaag ttgctccaag
    28921 cgttaacgga tttagaccaa aatcgtaata tgagccagtc ttttgaatca gtctcagatt
    28981 tgatggagga cttacgtgct taagattcgt tatcataaac agtttaaaaa agattttaag
    29041 ttggctatga agcgtggttt gaaggcagaa ttattagaag aagttttgaa ttttctggtt
    29101 caagaaaaag aacatcctgc cagatatcgt gatcattcat tgacggcatc caagcatttt
    29161 caaggagttc gtgaatgcca tacccagcca gattggcttt tggtttataa agtagacaag
    29221 tcggaattga ttttaaattt gctgaggaca ggcagtcaca gtgatttatt ttaatctatt
    29281 ttaagggggt tctcatgaaa ctaagaatat ttgcggaaga taagccggct aagaaggtat
    29341 ttgaatatca attagaactt gctgatcgta caattcttct atcgacagca ctcttgtcag
    29401 gtgctattgc tttagcagga atcttttctg ctttgagaga aaaataaaaa tagaaaagag
    29461 aaaacagaat ggttttacca aattttaaag aaaatctaga aaaatatgcg aaattgttgg
    29521 ttgcgaacgg aattaacgtg caacctggtc acactttggc tctttctatt gatgtggagc
    29581 aacgtgaatt ggcacatcta atcgtgaaag aagcttatgc cttgggtgcg catgaggtca
    29641 tcgttcagtg gacagatgat gtgattaacc gtgagaaatt cctccatgcc ccgatggagc
    29701 gtttggacaa tgtgccagaa tacaagattg ctgagatgaa ctatctcttg gagaacaagg
    29761 ctagccgtct tggagttcgt tcatctgatc caggtgcctt gaacggagtg gacgctgaca
    29821 agctttcagc ttctgctaaa gctatgggac ttgccatgaa gcctatgcgt atcgcaactc
    29881 aatctaacaa ggttagctgg actgtagcag ctgcagcagg acttgagtgg gctaagaaag
    29941 tcttcccaaa tgctgcgagc gacgaagaag cagttgattt cctttgggac caaattttca
    30001 aaacttgccg tgtctacgaa gcagatcctg ttaaggcttg ggaggaacat gcagccattc
    30061 tcaagagcaa ggccgatatg cttaataagg agcaattttc agcccttcac tacacagcgc
    30121 caggaacaga tttaacactt ggtttgccaa agaaccacgt ttgggaatca gctggtgctg
    30181 tcaatgcaca gggcgaagaa ttcttgccaa atatgccaac agaagaggtc ttcacagcgc
    30241 ctgacttccg tcgtgcagat ggttatgtca cttctacaaa accgcttagc tacaacggaa
    30301 atatcattga aggcattaag gtgaccttta aggatggaca aatcgtagat atcactgctg
    30361 agaagggtga tcaggttatg aaagaccttg tctttgaaaa tgcgggtgcg cgtgccttgg
    30421 gtgaatgtgc cttggtacca gatccaagtc caatttctca gtcaggcatt accttcttta
    30481 acaccctttt cgatgaaaat gcgtcaaacc acttggctat cggtgcagcc tatgcgacta
    30541 gcgttgttga tggagcggag atgagcgaag aggagcttga agctgcaggg cttaaccgtt
    30601 cagatgttca cgtagacttt atgattggtt ctaaccaaat ggatatcgat ggtattcgtg
    30661 aggatggaac gcgggtacct cttttccgta atgggaattg ggcaaattaa ggagataata
    30721 tgttaggaag tatgttcgtt ggtctcctag tgggattttt agcaggtgct atgaccaatc
    30781 gtggagagcg aatgggatgt tttggaaaaa tgtttctcgg ttggatcgga gcctttctag
    30841 gtcacttgct ctttggaact tgggggccag ttttatcagg aacagctatt atcccagcga
    30901 ttttaggagc catgattgtt ttagctattt tttggagacg aggaagttaa tttcctaaat
    30961 ct
//
LOCUS       DAVBNO010000021        29971 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVBNO010000021 DAVBNO010000000
VERSION     DAVBNO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29971
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.021"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            59..772
                     /locus_tag="VQ846_001060"
     CDS             59..772
                     /locus_tag="VQ846_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET3075644.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            786..1433
                     /locus_tag="VQ846_001061"
     CDS             786..1433
                     /locus_tag="VQ846_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075645.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCMGDFPHDFFDIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSN
                     FDDFLEKNYLKTIWQVSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            1571..2224
                     /locus_tag="VQ846_001062"
     CDS             1571..2224
                     /locus_tag="VQ846_001062"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET3075646.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTSWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            2234..2698
                     /locus_tag="VQ846_001063"
     CDS             2234..2698
                     /locus_tag="VQ846_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET3075647.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            2711..3979
                     /locus_tag="VQ846_001064"
     CDS             2711..3979
                     /locus_tag="VQ846_001064"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET3075648.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAAGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALFLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            3993..4253
                     /locus_tag="VQ846_001065"
     CDS             3993..4253
                     /locus_tag="VQ846_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075649.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            4264..5625
                     /gene="pdrM"
                     /locus_tag="VQ846_001066"
     CDS             4264..5625
                     /gene="pdrM"
                     /locus_tag="VQ846_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET3075650.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            5779..6747
                     /locus_tag="VQ846_001067"
     CDS             5779..6747
                     /locus_tag="VQ846_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET3075651.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            6763..7755
                     /locus_tag="VQ846_001068"
     CDS             6763..7755
                     /locus_tag="VQ846_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET3075652.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            7973..9016
                     /locus_tag="VQ846_001069"
     CDS             7973..9016
                     /locus_tag="VQ846_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET3075653.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQSNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            9080..10765
                     /gene="lpdA"
                     /locus_tag="VQ846_001070"
     CDS             9080..10765
                     /gene="lpdA"
                     /locus_tag="VQ846_001070"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET3075654.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASSVPVASTSNDDDKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDCIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAIAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIVDKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            10829..11818
                     /locus_tag="VQ846_001071"
     CDS             10829..11818
                     /locus_tag="VQ846_001071"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET3075655.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(11891..12961)
                     /gene="xerS"
                     /locus_tag="VQ846_001072"
     CDS             complement(11891..12961)
                     /gene="xerS"
                     /locus_tag="VQ846_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET3075656.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETAGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(13455..15005)
                     /locus_tag="VQ846_001073"
     CDS             complement(13455..15005)
                     /locus_tag="VQ846_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET3075657.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(15021..15800)
                     /locus_tag="VQ846_001074"
     CDS             complement(15021..15800)
                     /locus_tag="VQ846_001074"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET3075658.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(15787..16638)
                     /gene="ylqF"
                     /locus_tag="VQ846_001075"
     CDS             complement(15787..16638)
                     /gene="ylqF"
                     /locus_tag="VQ846_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET3075659.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            complement(16910..22123)
                     /locus_tag="VQ846_001076"
     CDS             complement(16910..22123)
                     /locus_tag="VQ846_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET3075660.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVVGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVG
                     TTGVDENGNLIEPPVNDIPEYTEPVGTTGVDENGNLIEPPVSDIPEYTEPISTVSEVA
                     SEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVRQVGEDGERQIVTSYEELHGK
                     KISDPVETVTILKEMKPKILVKGTKEKPKEKTAPVLTLDRTNTNVLNRSATLSYHLVN
                     TDGVTINKITATIKDGNEIVKTVDLTSEQLDKQVEDLKFYKDYKIETTMTYDRGKGEE
                     TATLEEKPLRLDLKKVEIKNIASTNLVKVNDDGTETPSDFMTEKPSDEDVKKMYLKIT
                     SRDNKVTRLAVDKIEEVTEEGKKLYKITAEAQDLIQHTDPTKVRNKYVHYIEKPVPKV
                     DDVYYNFKELVDAMNADKNGTFKIGADLNATNVPTPNKQYVPGTFKGHLSSVDGKQYT
                     IHNIARPLFDRVENGSVKNINLGNVDINMPWADGIAPVANMVKNATVEDVKVTGNVVA
                     NNNIAGIVNKIDSGGQLTNVAFIGNLTGVGDKGQYMAGIAGEIWRGNLAKAYVEADIV
                     ANRARIGGLVAKTDNGNDSMGIGKYGSIRKSVTKGTIKTKVLFETGGFINSNLPFGKL
                     EDNISMMRVENGEEFFGSSDLDYDGGYFTNGWLERNFVVKGVSSGKHSYKRSRDKIKE
                     ISQDEANKRIANFGLTADKYEINEPVVNRLNRLTRREDEYKSTQDYKSERDLAYRNIE
                     KLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYADGT
                     KEEMDVTKNTDSKVQQVREYSVSGLGDVVYTPNMVVKNRDKLIADVKSQLSSVELISQ
                     EVRDLMSRRDKPAENTDERKNGYIKDLYLEESFAEVKQNLDKLVKSLVENEDHQLNGD
                     EAAIKSLLKKVETNKAKIMMALTYLNRYYDIKYGDISIKNIMMFKPDFYGKTPSVIDR
                     LINIGSSEKNLKGDRTQDAYREIIAGNTGKSNLRNFLEYNMRLFTEDKDINDWFIHSA
                     KNVYVSEPKTTNTELKDKRHRVFDGLDNGVHGRMILPLLTLKDAHMFLISTYNTMAYS
                     SFEKYGKHTEEARNEFKTKIDEVAHAQQTYLDFWSRLALPNVRDRLLKSQNMVPTPVW
                     DNQTYNGSPVGRRGFDSKGNPIAPIRELYGPTWRHHDRDWRMGAMASIFPNPNNDDKV
                     LFMVTDMISPFGISAFTHETTHVNDRMLYFGGHKHRQGTDVEAYAQGMLQTPDSSTTN
                     GEYGALGINMAYHRPNDGNQWYNPDPDKLKTRDDIDRYMRNYNEAMMLLDHVEADAVL
                     PKIKGDNSKWFKKIDKEMRSKIQYNDLLGPNQWDSIRDLKDEEKVMTLSSVNDLVDNN
                     FMTKHGNPGNGRYRPEDFTPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGY
                     YGYENGFISYVSNKYKAEADKNNHGLLSDKLIINKVSKGNFNTLEEWKRHWYGEVLAK
                     AKKGFEAIDIDGVHISNYDELRPLFDKAVEEDLKKPDDFSHTVALKSKVFKALLKNTD
                     GFFNKLFKEDI"
     gene            complement(22167..28526)
                     /locus_tag="VQ846_001077"
     CDS             complement(22167..28526)
                     /locus_tag="VQ846_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET3075661.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDNETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYRDETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGALGKKVEIVRIFSVNNEEVSREIISTSTTAPVSRIVEKGTK
                     KAQVIKEQAETGAEHKEVQSGAIVEPAIQPELPAAVLTDKGESAVQPELPEAVVSDKG
                     VPEVQPALPEAVMTDKGDPEQVEPLPEYTGVQAGAIVEPEKVEPEYAGVQAGAIVEPE
                     QVAPLPEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVEPEKVEAPKEYTGVQAGAIVE
                     PEKVEALKEYTGKIEQPSAEDTKPNNENTNTPEEMSIQKKSSALINMNFITDSSKVTG
                     VGSATFIAPNVLLTVAHNFINNSTDNTTGEFRGDKSKNVYEWVTPDGQKGTFTANNIH
                     FYNKKDYPKGFIYDLAVIKLPETTGREHVELVKNYSKVNLNDKLNVHGYPAGKYTHLK
                     DATVEMEQEYANNTYGVQYQGGNPGMSGGGIFNANGEVIGVHQNGAQNRSGGLILSPT
                     QLAWIKSIIAGNEIPPVYDELYRHKDEKKDDAKDEKEVIKKLELRNISSVELYSKDGN
                     KYRHVTSLASLPSNAENYFMKVKSENFKDVMLPVTSITNDTKDNRDVYKIVASANSLI
                     QHENNNVLENYTYYLPKTQQSETGVYTSFKNLVDAMNSNPNGTFRLGATMDAREVELP
                     DGQESYVNNVFHGILVGTNNEKYYAIYNLKKPLFGELNGATVEKLSLKDVNISAKDDT
                     ATLAKEANNNTHIDNVHADGAIAGERSIGGLVSQVNNSTISNSSYTGRITNTYKTVAS
                     YQIGGLVGKLSGPRGLIDKSFASIDLSSNATQGDQSIGGIVGAVENSALISNSYAEGN
                     LNNVQRFANVGGVVGNLWDPVGGLEKSGRLSNVLSDVNVTNGNAIAGYNFNGIKANGT
                     YSNKNNKVVNVVQEDDEILTKDSTVQRGEVLEDAQIKEKKATFVSKNTIKTEDFNFSS
                     RYVTDYKNLENADSSKEKVYKNIEKLLPFYNRETIVKYGNLVETSSNLYNKELLSVVP
                     MKDKEVISDINKNKSSINKLLLYYADNTSETLNVNYQTDFSNVAEYRIGGTNLIYTPN
                     TLLRNYQNILDEVLPALNSVEYKSEAIRKVLDVSKDVSLTELYLEEQFNTTKTNLKDS
                     LTKLLTADAAIAENNNKVIDNYVIEKIKNNKEALLLGLTYLERWYDFKYRDTKAKDLV
                     MYHLDFFGKSNSSALDNVIELGKSGYNNLLAKNNVITYNVLLAKNYKTNNLFDALEKY
                     RKAFVPDKTNNEWFKEQTKAYIVEEKSTIKEVSDKQSIAGSPYSIGVYDRLTSPSWKY
                     PSMVLPLLTLPEKSVFIIANISTIGFGAYDRYRSKEHPAGTDLNDYVEKKAKEAAVRF
                     RDHYDYWYRILDDKNKEKLYRSVLVYDAFRFGTDEKEDKDTYQATFETNHPAIKHFFG
                     PAGNNVVHNSNGAYATGDAFYYMAYRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRNG
                     LGPEFYAKGLLQAPDHPNDPTVTINSILKYDQSEESTRLQVADPTQRFGSVDDLNKYM
                     HNMFDVIYMLEILEGKAVAKLDTNQKYDLLRKIENEYKPDPDGNSVYATNVVRRLKPE
                     ELTKLTTFNSLIEHDIITRRGYVDEATYKRNGYYTINLFSPIYSALSSKIGTPGDLMG
                     RRIAFELLAAKGYKDGMVPYISNQYEKEAKAQGKVITSYGKQIGLVTDEIVLSKVFNN
                     QYNSWIDFKKDMYKEREDKFGKLNKVSFIDPNGSWARQQKVTIDNINRLEKMIEDAVK
                     FDAEDEVAKLYPETNSRVLKLKKAIFKAYLDQTGDFRSSIFENKK"
     gene            complement(28735..29913)
                     /locus_tag="VQ846_001078"
     CDS             complement(28735..29913)
                     /locus_tag="VQ846_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075662.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         8696 a         5801 c         5573 g         9901 t
ORIGIN      
        1 tatgaggtgc tttatgatat aatgttctta atgaattttc agaaaggaaa acctcaaatt
       61 gttctacaaa tttctactct tcgacctcga ccacactctt cttgattttg atgctgctga
      121 ggatgtggct ttgacccaac ttctaaaaga agaaggagtt gcggatattc aggcttataa
      181 agattattac gttcctatga acaaggctct ctggaaagac ttggagctga agaaaatcag
      241 taaacaagag ctggttaaca cgcgcttttc tcgtttattt tctcattttg gacaggaaaa
      301 agacggtagt tttcttgccc agcgttacca attttatctc gctcagcagg gacaaacact
      361 atcgggcgct catgatctct tggacagcct cattgagcgt gattataact tgtatgctgc
      421 gacaaatggc attactgcca ttcagacagg acgtttggct caatctggtc tagtacctta
      481 tttcaatcaa gtctttatct cagaacagtt gcaaactcaa aagccggatg ctctctttta
      541 tgaaaagatt ggccagcaaa ttgctggatt tagtaaagaa aagacgctga tgattggaga
      601 ttctctaacc gccgacattc aaggtggcaa taatgcgggg attgacacta tctggtataa
      661 tcctcatcac ctcgaaaatc acacacaagc ccagccgact tacgaagtct attcttacca
      721 agacttgctg gattgtttag ataaaaatat tcttgaaaag atcacatttt aaaggagatt
      781 agctaatgac tacaaaaaaa gctaatatta ctattgaaga gtacattgaa atgtctgaag
      841 ttgattttaa tgaagctgtt aattatgaat ttacatctga cacttgtcaa ttagcaaata
      901 gtatttatca atctcttttt aagttttttg ataagaaaaa tttctctggc gatttaattt
      961 ttacttggaa atctccatca ttagtcaaag aaggggatta tattgggaga agggattcac
     1021 aagtagataa tcttagagta ataggaaata tatttccgaa ttatcttact aatcgaaaat
     1081 atagcctcaa tatgaatcgt aatggctgta tgggagattt tcctcatgac ttttttgata
     1141 tatacctaga tcatgtagca aaatatgcct acgaacaaaa agttaataat attaaagagt
     1201 attatccttt aaaaagagcg attttacacc aagagaatgc attgtatttt cgattttttt
     1261 ctaattttga cgacttttta gaaaaaaatt atttaaagac tatatggcaa gtttctaaag
     1321 aaactccttt ttctgaaatg gattttaata tgtttaaaaa tatttcagaa aagataatct
     1381 tcgaaagagg tagtaaaatg cttaatgatt taaaaagtaa ttataaaaaa tagccagtct
     1441 ttataaactg tattcacgta ttcaaatcgt atatatagtc gatttattaa atctttagat
     1501 aaactgtaaa aagtggtttc catagccact ttttgctata atagaggcag taaaaatgaa
     1561 ggagtcggct atggaacact cgtcttggca tgctttgatt aaggcgcaat tacctgaggg
     1621 ttatttcggg aaaatcaatc agtttatgga gcaggtctat tctcagggga ttatttatcc
     1681 acccaaggaa aaggtttttc aggctctctt gacaacactg cttgaagaag ttaaggtggt
     1741 aattctaggg caagacccct atcacggacc aggtcaagcg cagggcttga gtttttctgt
     1801 acctgactct atcccagctc caccatcctt gcaaaatatc ttgaaagaat tgtcagatga
     1861 tatcggagtt aagaaatctc atgatttgac atcttgggct gagcaaggag tcttgcttct
     1921 taatgcttgt ttgactgttc ctgctggaca ggccaatggt catgctggtc aaatctggga
     1981 gccttttact gatgctgtga ttcaggtggt caatcatcta gatagaccag tcgtttttgt
     2041 actctgggga gcttatgcac gtaagaagaa ggcattagtt accaatcctc atcacttgat
     2101 tatcgaatca gcccatccaa gtcctttgtc ggtttataga ggattttggg gttccaagcc
     2161 cttttccaag gccaatgcat tcttaaaaga gacaggacaa gagccaatcg attggcttag
     2221 ataaggagaa aatatgcctc agttagcgac gatttgctac attgataatg ggaaagaact
     2281 gctcatgctc catcgtaata agaaacccaa tgatgtccat gaagggaaat ggattggtgt
     2341 gggtggtaag ctagagagag gagagacgcc ccaggaatgc gcggcgcgtg aaatccttga
     2401 agaaacaggg ctcaaagcca agccagttct aaaaggtgtc atcacttttc ctgaatttac
     2461 accagattta gactggtaca cctatgtttt taaggtgacg gagtttgagg gcgacttgat
     2521 tgactgcaat gaggggacgc tagaatgggt tccctatgat gaggttttga gcaagccgac
     2581 ttgggaaggt gaccacacct ttgttgagtg gcttttagag gataaaccct tcttttcagc
     2641 caagtttgtt tatgatgggg ataaattgtt ggatacccaa gttgatttct atgaataaag
     2701 gagaaagcag atgctactaa tcaaaaatgg tcgtgtaatg gatcccaagt ctggtttgga
     2761 tcaagtttgt gatgtcttag ttcaagatgg gaaaattatc aaaattgcgc ctgagatcac
     2821 ggaagaagga gcagaaacga ttgatgctac tggtcttgtg gttgctcctg gtttggtcga
     2881 tattcatgtg catttccgtg aacctggtca aacacataaa gaagacattc atactggtgc
     2941 cctggcagcc gctgcaggtg gttttactac tgtcgtcatg atggctaata ccagtccaac
     3001 catttcagac gtggagactt tgcaagcagt tctccagtca gctgccaaag agaagattaa
     3061 tgtcaagaca gttgcgacca ttactaaaaa ctttaatggt aaaaacttga ctgactttaa
     3121 ggcgctctta gaagctggtg cggctggttt ctctgatgac ggtattccgc ttgagagcag
     3181 taagattgtc aaggaagcca tggaggaagc caaaaaactc aataccttta tcagccttca
     3241 tgaggaagat ccaggtttga acggtgttct tggctttaat gaaaatattg ctagagaaca
     3301 tttccatatc tgcggtgcta ctggggtggc tgagtacgct atgatggcgc gtgatgtcat
     3361 gattgcctat gcaactaaag cccatgttca catccagcat ttgtctaagg aagaaagtgt
     3421 taaagtagtg gagtttgctc aggggttagg tgcagaagtc acagcagaag tagcgccaca
     3481 gcatttctct aagacagaag cacttttttt aacacaaggt agcaatgcta agatgaatcc
     3541 accgcttcgt ttggaatcag accgtcgtgc cgttatcgaa ggtctcaaat caggtgtcat
     3601 cacagttatt gcgactgacc acgcccctca tcatgtagat gaaaaaaatg ttgaggatat
     3661 taccaaagcg ccatctggta tgactggctt agaaacatcc ctgtctctca gcttgaccta
     3721 tttagtagaa gctggtgagt tgagcttgat ggaattactt gaaaaaatga catacaaccc
     3781 agccaagctt tacaactttg aagcaggtta cttggctgag aatggtccag cagatatcac
     3841 tatttttgat gccaaggctg accgccttgt ggactcccat tttgcttcca aagcagctaa
     3901 ttcaccattc atcggtgaaa ccttaaaagg gcaggttaaa tataccatct gtaagggaca
     3961 aatcgtctat caagcttgat aggagttaga atatgaatta ttttcgaaaa cgtagggaga
     4021 gacaagcgaa atctaatagt ggaatttact gtcccgcagc taatcgttcc aaaattcaat
     4081 atgaaactaa agaaaaagct gaccatgcgg ttcaatatga taacggaggt ttagttagaa
     4141 gctattattg tcgtacatgt gcgtgttggc atactacttc aaaatctaat aaacctccgc
     4201 taagtttgaa aaactatggt ctgaaactct ttggtttaga taaatcagaa tagaaagaga
     4261 agtatgtata agacaaagtg tttacgagag aagttagtat tatttttaaa aattttcttc
     4321 ccaatcctga tttaccaatt tgccaattat tctgcctctt ttgttgatac tgcaatgaca
     4381 ggtcaataca acactatgga cttggctggt gtatctatgg caaccagtat ctggaatcct
     4441 ttctttacat ttctaacagg gattgtgtca gccttggtgc ctatcattgg tcaccatctt
     4501 ggtcgaggaa aaaaggaaga agttgcgtct gatttttacc aatttattta tttggccttg
     4561 ggcctatctg tggtattgct ggggatggta ctttttttgg caccaacaat cttgaatcat
     4621 attgggttag aagcagcagt agcggcagta gcggttcgct atctttggtt tttatctatc
     4681 gggattatcc ccttgttgct ctttagcgtc attcgttcct tgctggattc gctgggcttg
     4741 accaaactgt ccatgtacct catgcttttg ttactccctc tcaatagcgg atttaactat
     4801 ctcttgattt acggtgcctt tggtgttcca gaactgggag gggctggtgc tggtttagga
     4861 acatccttgg cctactgggt cttgcttggg atttctgttc tggttttatt taaacaggag
     4921 aagctcaaag ccttacacct tgagaaacga attccactta atatggataa aattaaggaa
     4981 ggagttcgtt taggtctgcc tattggggga actgtcttcg cggaagtggc tatcttttca
     5041 gtggttggct tgattatggc taagttttcg tccttgatta tagctagtca ccagtcagct
     5101 atgaactttt caagtcttat gtacgccttt cctatgagta tctcatcggc tatggctatt
     5161 gtcgtttcct atgaagtggg agccaagcga tttgatgatg cgaaaaccta tattggtcta
     5221 ggaagatgga ctgccctcat ttttgcggcc ttcaccttaa ccttccttta catttttagg
     5281 ggaaatgtgg ccagtcttta tggtaacgac ccaaaattta tcgatttgac agtgcgtttt
     5341 ttaacttata gtcttttctt ccagttagca gatacctttg cggcgccgct tcagggaatt
     5401 ttgcgggggt ataaggatac agttattcct ttttaccttg gtttgcttgg ttattggggc
     5461 gtagcaatcc ctgtggctac gctatttgat tctctaacag attttggagc ttattcttac
     5521 tggatcggct tgattattag tttgattgtg agtggggcgc tctaccgttg gcgtttaact
     5581 gtgattatga agagatttga atctttagca aaatccaaac gctaaaaagt ttgtgaaaaa
     5641 atattcttga taagaaaatc ccttgttttc acggggtttt cttttttatt ttgtgaaatt
     5701 tcttttagaa aaactttgac agtatttgcc aaaaacggta aaatagtaag gtaagaagaa
     5761 agttagaaag gcaagaagat gtcaacttta gataaaaatc ttttgctaga aatgttccgt
     5821 aagatggaag aaatccgtcg catggactta aaaattgcac aattagtaaa gaaagggaaa
     5881 gtgccaggaa tgacgcactt ttctgttggt gaagaggcag ctaacgtggg ggctatgtta
     5941 gctctcaatc cagatgatct gattacctca aaccaccgtg gacacgggca agctattgct
     6001 aaagggattg acctcaacgg aatgatggct gaaatccttg gtaaatacac tggaacctgt
     6061 aaagggaaag gtggatctat gcatatcgct gaccttgatg ctgggaacct tggtgccaat
     6121 ggtattgtag gtggtggtat ggggatcgct gtcggtgcag ccctcagtca gcaaatgcaa
     6181 aataccggta aaatcgttgt ctgcttcttt ggagatggtg cgaccaatga aggtgttttc
     6241 cacgaagcag tgaacatggc ttctatctgg aacctgccag tcattttcta ttgcattaac
     6301 aacggttacg gtatctctgc ggatatcaag aaaatgacca atatagaaca tatccatcaa
     6361 cgtagcgccg cttatggaat tcctggaatg ttcatcgaag atggtaacaa tgtcatcgat
     6421 gtctatgaag gatttcagaa agctgtggac catgttcgca gtggcaatgg tccagtcttg
     6481 attgaaagtg taacttatcg ctggctcggt cactcatcat ctgaccctgg taaatatcgt
     6541 acgcgtgaag aagtggaatt gtggaaacaa aaagatccaa tcgaaaacct ccgcaattac
     6601 cttattgaaa ataacattgc aagtgccgaa gaattggaag aaatccaagc gcaagtaaag
     6661 gaagcagtag aagcttctgt taaatttgca gaggaaagcc cattcccacc gcttgaatca
     6721 gcctttgaag atatttacgc agactaagga gaaagagata aaatggaaac aaaaacaatg
     6781 tccttccgtg acaccattat ccttgctatg tctgaggaaa tgcgtcgcga tgaaaatgtg
     6841 ttcttgatgg gagaagacgt cggtgtcttc ggaggagact tcggtacttc tgttggaatg
     6901 cttgaagaat ttggtccaga acgcgtccgt gactgtccga tttctgaagc tgccatctct
     6961 ggagcagcag caggagcagc catgacagga cttcgtccaa tcgtcgatat gaccttcatg
     7021 gacttctctg ttattgccat ggacaatatc gtcaaccaag ctgctaaaac acgttacatg
     7081 tttggtggta aaggtcaggt tccaatgact gttcgttgtg cagctggtaa cggagttggc
     7141 tctgccgccc agcactcaca atctctagag tcttggttta ctcacattcc aggacttaag
     7201 gttgtggcac ctggaacacc tgcggacatg aaaggcctgc ttaagtcttc tatccgtgat
     7261 aaaaacccag ttattatact tgagtacaag tcagaattta accaaaaagg ggaagtgcca
     7321 gttgatccag actacacaat tccacttggg gttggtgaaa tcaaacgcca aggaacggat
     7381 gtaacagttg ttacttatgg aaaaatgctt cgccgtgtgg ttcaagctgc tgaagaatta
     7441 gcagaagaag gaatttcagt tgaaattgtt gacccacgta cccttgttcc gcttgataag
     7501 gatatcatca taaactcagt gaagaagact ggtaaggttg ttctggtcaa cgatgcccac
     7561 aaaacaagtg gctatatcgg tgaaatttca gctattattt cagaatcaga agcatttgac
     7621 tatctagatg caccaattcg ccgttgtgca ggagaagatg tgccgatgcc ttatgcacaa
     7681 aacctagaaa atgcaatgat tccaacagtt gaaagtatca aagatgcaat ccgaaaaact
     7741 tataacaaag aatagagtta cataagataa gttatgtaaa taaaataaag ctgacgagaa
     7801 actttgtatc ttttaggtgc agagctcttc gtcattaata tcttagatta gagcacgggc
     7861 taaaagctcg gaaaaaagat aaatctcctt ggcttcatcg aagccatcgt cgatttccta
     7921 tttttcagtc gcttttgacg ccctttgtat cataaataga attggagagg tcatggctga
     7981 tgacaagcta agagcgactc ctgcagctag aaagttagcg gatgatttag ggataaacct
     8041 ctacgacgtt tctggctcag gtgcaaacgg tcgtgtccac aaagaagacg tggaaactta
     8101 taaagacaca aacgtggttc gcatttcgcc acttgcaaaa cgaattgccc tcgaacataa
     8161 cattgcttgg caggaaatcc aaggaaccgg tcatcgtggt aaaatcatga agaaggatgt
     8221 tttggccctg cttcctgaaa atatcgaaaa cgatagcatc aaatctcctg ctcagattga
     8281 aaaagtggaa gaagtccctg ataacgtaac accatatggt gaaatcgagc gtattccaat
     8341 gacaccaatg cgtaaggtta ttgcccaacg catggttgaa tcttacttga ctgcgccaac
     8401 cttcaccctc aactatgaag ttgatatgac tgaaatgttg gctcttcgta agaaggttct
     8461 tgagccaatc atggaagcaa ctgggaagaa gactactgta acagaccttc tttcgcttgc
     8521 agttgttaag actcttatga aacacccata catcaacgct tcattgacag aagatggcaa
     8581 gactattatc actcacaact atgtcaatct tgccatggca gttgggatgg ataatggatt
     8641 gatgacacct gttgtttaca atgctgagaa aatgagtctt tcagaactgg ttgtagcctt
     8701 taaggatgtt attggccgta ccttggatgg taaattggct ccaagtgagt tacaaaattc
     8761 aacattcaca atcagtaatt tgggaatgtt tggtgttcag tcctttggtc cgattattaa
     8821 ccaatccaac tcagctatcc ttggtgtcag ttcgacaatc gagaagccag ttgtcgtcaa
     8881 tggtgaaatt gtgattcgcc caatcatgag tttaggatta accattgacc accgtgtcgt
     8941 agatggtatg gctggtgcta agtttatgaa ggacttgaaa gagttgattg aaacgccaat
     9001 ctcaatgtta atttaagagc aagagtattc atttttaaga tatagataaa ggaaggaaga
     9061 catggcctta gaagtaatta tgccaaaagc cggcgtggat atgacagaag gacaaatcgt
     9121 ccaatggaat aaaaaagtcg gagaatttgt aaaagaagga gaaatccttt tggaaatcat
     9181 gactgataaa gtcagcatgg aattggaagc cgaagaagat gggtacttga ttgccattct
     9241 caaaggagat ggtgaaactg tccctgtaac ggaagttatc ggttaccttg gtgaagaaag
     9301 ggaaaacatc ccaacagctg gagcagcatc gccagaagct agctctgtac ctgtagcaag
     9361 tacctcaaac gatgatgata agagcgatga tgcttttgat atcgttgtga ttggtggagg
     9421 tcctgctggt tatgttgcag ccattaaagc tgcccaattc ggcggtaagg ttgcccttgt
     9481 tgagaaatct gaacttggtg gaacctgctt aaaccgtgga tgtattccaa ccaagaccta
     9541 ccttcataac gctgaaatta ttgaaaatat cggtcatgct gcaaatcgtg gtatcgtgat
     9601 tgaaaatcct aacttcactg ttgatatgga aaaactttta gaaactaaat ctaaagttgt
     9661 taatactctt gttggtggtg ttgcaggact tcttcgtagc tacggagtta ctgttcataa
     9721 aggaattggt acaatcacta aagacaagaa cgtcttggta aatggctctg aattgcttga
     9781 aaccaagaaa atcattcttg ctggtggttc aaaagtcagc aagatcaacg tccctggtat
     9841 ggaatctcca cttgtcatga ctagtgatga cattcttgaa atgaacgaag tgccagaaag
     9901 ccttgttatc atcggtggtg gagttgtcgg tatcgaactc ggtcaggcct tcatgacatt
     9961 tggttcaaaa gtgactgtta tcgaaatgat ggactgtata gttccagcta tggatgcgga
    10021 agtttctaag aaccttcgct tgatccttga gcgtaaagga atgaccatct tgactggtac
    10081 taaactgcaa gaaatcattg aggaaaatgg tcaacttcgt atcaaggttg aaggaaaaga
    10141 cgatatcatc gcaagcaaag ctcttctttc aattggtcgt atgccagacc ttgaaggtat
    10201 tggagaggtt gagtttgaat tggatcgtgg ttgtatcaag gtcaatgaat acatggaaac
    10261 ttcagttcca ggcatttatg caccaggtga catcaacggt actaagatgt tggctcacgc
    10321 agctttccgc atgggtgaag tttccgctga aaatgccctt aaaggaaatc atgcaattgc
    10381 caaattgaat ttgactcctg cagccatcta cactctccct gaagtagcag cagtaggttt
    10441 gacagaagaa caagcccgtg agaaatacga tgttgccatc ggtaagttta actttgctgc
    10501 taacggtcgt gctattgcat ctgacgctgc tcaaggtttc gtaaaagtta tcgttgataa
    10561 gaaatacgga gaaatccttg gtgtacacat tattggtcct gcagccgcag aattaataaa
    10621 cgaagcatca agcatcatcg aaatggaaat cactgttgag gaaatgctga agaccatcca
    10681 cgggcaccca acttactctg aagtgatgta cgaagcgttt gcagatgttc taggaatggc
    10741 catccattca cctaagaaaa aataaaacga agatagatta gactggaagg ttatttccag
    10801 tttctatcat ataaagtaaa gggatatcat gaaatacatt atcaatcatt caaatgacac
    10861 tgcttttaat attgccttgg aagaatacgc ctttaaacac cttttggatg aggatcaaat
    10921 cttcctactt tggattaaca agccatctat cattgttggt cgtcaccaga acactatcga
    10981 agaaatcaac cgtgattatg ttcgtgaaaa tggtattgag gtagttcgcc gtattagtgg
    11041 tggtggagct gtttaccacg atttaaataa cctcaactac acgatcatct ccaaagaaga
    11101 tgaaaataag gcatttgact tcaagagctt ctcaactccg gttatcaata ccctggctca
    11161 acttggggtt aaagctgagt tcacaggtcg taatgacctt gagattgatg gtaagaagtt
    11221 ctgtggcaat gcccaagcct atatcaacgg ccgtatcatg caccacggtt gcttgctctt
    11281 tgacgttgat ttgtcagtcc tcgcaaatgc ccttaaggtt tcaaaagata aatttgaatc
    11341 aaaaggtgtg aaatccgtcc gtgcccgtgt aaccaatatt atcaatgaat taccaaaaaa
    11401 aatcacagtc gaaaaattcc gtgatttact cttggaatac atgaaaaaag agtacccaga
    11461 gatgactgaa tacgtctttt cagaagaaga attggccgaa atcaatcgca tcaaggatac
    11521 taagtttgga acttgggact ggaactatgg taaatcacct gaatttaacg tccgtcgtgg
    11581 aaccaaattc actagtggta aggttgaagt ctttgctaac gttactgaat caaaaatcca
    11641 agacatcaag atttatggtg acttctttgg tattgaagac gttgctgcag tagaagatgt
    11701 ccttcgtggg gtgaaatacg aacgcgaaga tgttcttaag gcgctaaaaa ccattgatat
    11761 cacacgctac ttcgctggta ttagccgtga agaaatcgct gaagcggtag ttggataaat
    11821 caaaaagaag ttggccaact aaccaacttc tttttttgat atttatttac ataatttaaa
    11881 atacgtaaaa ttataaacta tctagagcat tcttttgttc atcattaaca atatgggtat
    11941 agaggtcagt gacttgtgtg ctggcatgtc ctagttggtg actgactaaa acttgtgatt
    12001 tagtcgcatc atagagccta gttgctagcg tatggcgcag tttatggggt gttacacgca
    12061 ctttaaaatc ctctgagtat ttagcaacca ttttctcaac gctagaagca tcgatacgat
    12121 taggaacacc tctgtagaga gttaaaaaaa gggctgtatc tgttttttcc gttttatagc
    12181 gttgattccg aatggccaga taattctcta aataaggttt agcaaaagca gcgacattga
    12241 ctgagtcacg tttgccacct tttcgagtaa catcaataac catcattttt agattgagat
    12301 ctcttagatc tagattaaca gcttcagata agcgaacacc agatgccaag agaagggcaa
    12361 taatggctaa atctcgttct ttatttttgt tgaatgatga gagagctcga tttgaaagct
    12421 gttgtgggtg ctcttgatcg atataagtta gaaaacctgc tgtttcatca cctagaaaga
    12481 gtttttgctt gatattttca gctctggcag caagggtttc tttctttttc ttggttgaaa
    12541 cttttttcat tacattacga tagaaataag gttccccctg atcattttca acctcctcgg
    12601 ttagatactt gtaaagacta gaaagtgctg ataaggttcg attgatagtt gtctgtgaaa
    12661 caccttgttt tgttgtatta gcattcagca agggacgttc acgtagataa aggataaagg
    12721 attccatgtc tttcttagac atattttcca aaactgataa aggaatatcg gatattttat
    12781 cagcgtttga aataccagac tccaaaaccc agctgaaaaa tcggtcatat tccttaaggt
    12841 attcgtacag ggttgtaaaa ctgtagggca cagccagttt agattggtag tattccagaa
    12901 cataccaggg catgagttgt tttagtttgt cgattcgttc cagtaaaatc tcacgtttca
    12961 tgtattttct ccataattaa gtatactagt agtatagcat agacttgtat atttttcaag
    13021 aaaattatag tttttcagaa agatgttata ggagtttttc aaagtgataa aaagacttag
    13081 acctacaaac aaaacagaat aaggaaaata ataaaaatct cacccactaa acttctgtta
    13141 atactacata gctagaaatt gatttgattg tcctgattgt tttatcctat ttctatttca
    13201 tttcactata taattgctat tgttttcttt ttcatgattt taatcttact gtacagattt
    13261 gatacagtaa actcctttct agctttatgc tttatcattt ctaacttaat tatagtctta
    13321 tttttaataa agttcaatta tttatatgta aaattcctca tcagtaaaaa ataaccgatc
    13381 tcatttctga gaatcggttt tctaaataaa tcaaaccgat catttacata acataaatta
    13441 tgtaaatgat catactacaa caacaaatct ttgacttttc caatttcact ttttggaata
    13501 accaggtgaa tcatatcacc cagatacatt ctggttgagc cgttaactgt ttggctcttg
    13561 ccattatgga cttgagttgt gatgaggacg ttgtgtggta agttgagttc atgaacttgt
    13621 ttcccagcaa ttttatcaga aactggtatt tcgataagtg taacttctcc ttcgctagat
    13681 acttcttctg gaagcatttt ttccagcatg gcttcataga ctggcgtacc tttgagcaaa
    13741 tccatgataa tataagaaac aagagtgaca agaccaagtg gcataaggtt gcgaatatct
    13801 cctaccatct cagttacgag gatcatagcg gttaagggag cttttgatat ggctccaaaa
    13861 tagccactca ttcctagaat gacaaatata gggaattgct cttgactgac aagtccaaga
    13921 ttgacacaga taacaccaac taaggcacca agcaaagaac caagagctaa aatggggagg
    13981 aaaattcctc ctggcagtcc acttccatag ctaatcatac tccaaataaa gcggattaaa
    14041 aagtaagcta ataaaacttg gaaactaaaa ttttgttcag ttaaagaaag gacaagctga
    14101 tttccgccac caatgatttg aggtaagaag attccgactg gtatgataag gataaaagcc
    14161 aagatgggat aatatgccct atccaaatgg attttttgac caatcaagtc ataaactctt
    14221 ccaacgttta atacagcttt ctcatagaga aaacctgaaa atccaaggaa aattcccatg
    14281 acgagatata tccaatactg gtctagggtc atgggaggaa tgttatctgg catatccaat
    14341 actggtgtca aaccgaacat gagtagagac acaaagtttg ctacgatgct ggctgctaga
    14401 gttgagaccc agaaaaagcg cgaaaaatgg tgatagactt cttctacaac aaagagaagt
    14461 gctgcaatag gagcattaaa ggctgcggct aaacctgctg cagctccact ggcaatcaag
    14521 gaacgttcct ctactggact ggatttgagc cacttggcaa ttcctttacc accaactgct
    14581 ccaagttgaa tgctgggtcc ctctcgaccc agcatgagtc cactggcaat agcaagaata
    14641 cctagcacat attttttcca aagaatgccc caccagttga gggacatgag gcctttcagt
    14701 tcggcttcga cttgaggaat tcctgagcct ttaatatctt tttctgaccg tgttagtttg
    14761 gcactgagcc aacagatgag tatataaaac aaaaccagta caaaaagatt gcgcactaag
    14821 tacccttgat cttgataaac tccttgtatc aggtggaagc ccttttcaat taagaaacga
    14881 aaggatccga cgataattcc aacgatgagg ccgacaatga ttcctcgacc aacttgggat
    14941 agtatagtgc tggatgcaaa ggcgaattct ttcttagaac ggactatttc cgactgttcc
    15001 tccatggtat ttcctttcaa ttaactttct tttttaccta aaaccagtga tttaacgggt
    15061 tcaaagctgg ttcggtgaat tggggtaact cctagttttg tgaggccttc cagatgttta
    15121 gctgtgccat atcctgcatt agtagcgaaa tcatagccag agaactgctg atcgtattct
    15181 ttcatcaatt catcacgtgt taccttggct actatagatg ctgctgcgat agagagggag
    15241 ttggcatctc ctttgataat ggaggtttgt gaaatgggca agtccagttt catggcatca
    15301 atcaaaaggt gctctggttg agggctgagc tgggagattg cttcttgcat ggctagtttg
    15361 gttgcttcat agatgttgac ttggtcgatg acctgattat ctatgatacc aattccaatc
    15421 gacaaggctt ggtcttgaac ggcttggaaa atctccagat gtttcttttt aggaattttc
    15481 ttgctgtcgt tgagaccttt aatcttacaa tttttaggta aaataacggc cgcagcgact
    15541 acaggaccag caagaggacc acgaccaacc tcatcaatac ctgcaattaa ggtcaatcct
    15601 tgcttataaa gttctttttc ataagaaagc atggattcca agcgcaaatt ttcatctaat
    15661 tcagcttgaa tggctctttt acgcttgctg atttcctttt gaactcctga gcgattatcc
    15721 ttttcaagct ctaaaaaaat agggctttct aactccttga ctgtcacaag gagttctttg
    15781 atttctttaa tcgttgccat cgaggtcttc caatgtatct aaggtatagt taccgagttt
    15841 gccatcacgg acttccttca cgaagagact gtaaaaacgg tcatagtcat cacggaaacc
    15901 gagggcgcgg gtcatatcca taataatcac aggcgcttct tcttcaattt tcatttgttt
    15961 gaagcgttca gccagctttt ctggataatg ttctttgaaa taattgatac caaaaatggt
    16021 aacctcatcc ataggaagca actgatcctt gatagctcca gtcaatgcca acttaagtgc
    16081 aacagtttca tcctcaaact taggccagag aatccccggt gtatccaaga tttccagatc
    16141 tttattggtt ttaagccatt gttgaccttt tgtgacccct ggcttgtttc caacaacagc
    16201 aatcttttta ccagccaaac ggttcatcag agttgattta ccagcgtttg gaatcccaat
    16261 aatcatggta cgcaaggttt caatctgaat cccacgttct ttctggcgag caatcttatc
    16321 agccatgagc ttcttggccg catctgttac aacttttaca gtcacttgct ctttggagtt
    16381 gatagctagc gtctggattc cttgtgattc aaaatactga cgccattcct tggtcattgc
    16441 tggatcagcc aagtcggcct tgtttaaaat caagagtttt ggtttatcac caacaatctt
    16501 ggtcaacata ggattttgac tagatagagg caagcgtgca tctactaaaa tcgtcacaaa
    16561 atcaacaaat tttaaatttt cctgcacctg tcgacgcgct ttagacatgt gaccaggaaa
    16621 ccattgaata gtagccattg agttttttcc tttcgtagca aggatttaga tcccctattt
    16681 tattttacta tagtttgaac atagagagtt ttcaaatgaa tgatgtccaa acagttcttg
    16741 ttttatgaca tagaaatcta ctttatatta tatcatgcct tctttatttt gtccgatgat
    16801 agccttttac cctatctttt agcctatctt ttaccctata tagaaaaaag ccatcaaatg
    16861 atgacttttc agagggagca aaaaactgtt ccttctatat tctaaacatt taaatgtctt
    16921 ctttaaacag tttattaaag aaaccgtctg tgtttttaag aagggctttg aagaccttag
    16981 atttaagagc aacagtgtga gagaagtcat ctggtttctt caaatcttct tcaactgctt
    17041 tatcaaataa aggccttaac tcatcatagt tactgatatg gacgccatca atatcgatag
    17101 cctcaaaacc tttcttagct ttagcaagaa cttccccgta ccaatgtctc ttccactctt
    17161 caagagtatt aaagtttcct ttcgatactt tattaattat tagcttatcg cttagtaaac
    17221 cgtggttgtt tttatctgct tctgctttgt atttatttga tacataacta ataaagccat
    17281 tttcataacc ataatagccc cacatacgga aagcattgtg cttaaatgat aatgaaccag
    17341 gagcaccttg actcgtattc ccaccgtaaa ttcctgccat catattgaca tttacgtaag
    17401 ctgagtttgg tgtaaaatct tctggtctat aacgtccatt acctggattt ccatgttttg
    17461 tcatgaagtt attatccact aaatcattga cgctagataa agtcattacc ttttcttcat
    17521 ctttcaaatc acggatacta tcccattggt ttggacctaa aagatcatta tactgaatct
    17581 tagagcgcat ttctttgtca atcttcttga accacttact attgtctcct ttaattttag
    17641 gaagtacagc atcagcttct acatgatcaa gcaacatcat agcttcattg tagtttctca
    17701 tgtagcggtc aatgtcatca cgtgtcttta atttgtcagg atctgggttg taccattggt
    17761 taccatcatt tggacgatgg taagccatgt taattccaag tgcgccgtat tcaccattag
    17821 tcgttgagct gtcaggtgtt tgcaacatac cttgagcata ggcttcaacg tctgtacctt
    17881 gacgatgctt atgaccaccg aagtaaagca ttctatcgtt tacgtgtgtt gtttcgtgag
    17941 taaaggctga aataccaaat gggctaatca tatccgtcac cataaacaag accttgtcat
    18001 cattatttgg attagggaat attgatgcca tagcgcccat acgccagtct ctatcatggt
    18061 gtcgccatgt tggaccatat aattcacgaa ttggagctat agggttacct ttactgtcaa
    18121 atcctcgacg acctacagga gaaccattgt aggtctggtt atcccaaact ggtgtcggta
    18181 ccatattttg actcttaaga agtctatcac gaacattagg cagagctaat ctggaccaga
    18241 aatctaaata agtttgttga gcatgtgcaa cttcatcaat cttagttttg aactcattac
    18301 gagcttcttc tgtatgtttt ccatatttct caaatgaact gtacgccatc gtgttatatg
    18361 ttgaaattaa gaacatatgg gcatctttaa gcgttagaag tggtaaaatc atacgaccat
    18421 gaacaccgtt atctaaacca tcgaacacac gatgacgttt atctttcaac tctgtatttg
    18481 ttgtcttagg ttctgaaaca taaacattct tagctgagtg gataaaccaa tcattgatgt
    18541 ctttatcctc tgtaaacaga cgcatgttgt attctaagaa gttacgcaaa ttacttttac
    18601 cagtattacc tgcgatgatt tcacggtaag catcctgagt ccgatcccct ttcaagttct
    18661 tttcacttga accgatgttg atgagtctgt caataacact tggtgtttta ccataaaaat
    18721 caggtttgaa catcatgatg ttttttatac ttatgtcacc gtatttgata tcatagtaac
    18781 gattgagata agtcagagcc atcatgattt tagctttatt ggtttccact ttcttgagaa
    18841 gtgattttat cgcagcttca tcaccattca attgatggtc ttcattttcg accaaggatt
    18901 tgactaactt atctagattt tgcttcactt cggcaaagct ttcttctaag tatagatctt
    18961 taatataacc attcttacgt tcatccgtat tctctgcagg tttatcacgt ctactcatta
    19021 aatctcttac ttcttgtgaa atcaattcca cacttgataa ttgagattta acatcagcaa
    19081 tcaatttatc acgattttta acaaccatat tcggtgtata aacaacatca ccaagaccag
    19141 atacactata ttcacgcact tgttggactt tagaatcagt attcttagta acatccattt
    19201 cttccttagt accatcagca tagtgaacca taatcttatc aatatctgat aaatcggtca
    19261 cgaattgacc atccttcatc cccgtaacag aaaggacttc tttcttagct aagtttgaat
    19321 cttctgcaag tttgttacct tggttgacaa tccattcttt attataaaat ggttgaagtt
    19381 tctcaatatt acgataagct aaatcacgtt cggatttgta gtcttgagtt gacttatatt
    19441 catcttctct tctggttaaa cgatttaagc gattgaccac aggttcgttg atttcgtatt
    19501 tatcagcagt aagtccaaaa tttgcaattc tcttattagc ttcatcttga ctaatttctt
    19561 taattttgtc acgagaacgt ttgtatgaat gcttacctga actgacacct ttgacaacaa
    19621 agttacgctc taaccagcca tttgtgaaat aaccaccatc gtagtccaaa tcggaagaac
    19681 caaagaactc ttcaccattt tcaactctca tcattgaaat attatcttca agtttaccaa
    19741 aaggaagatt gctattgata aatccacctg tttcaaacaa aactttagtc ttaatggttc
    19801 ctttggtgac tgatttacga attgaaccgt atttaccaat tcccatagag tcgttaccat
    19861 tatctgtttt agcaactaaa ccacctatac gggctctgtt agcaacaata tcagcctcga
    19921 catatgcttt tgcaagatta cctctccaaa tctcaccggc aatgccagcc atatattgac
    19981 ctttatcacc aacacctgta agattaccga tgaaggctac atttgtcaac tgaccaccac
    20041 tatcgatttt attgacgata cccgcaatat tgttattagc aactacatta ccagttacct
    20101 taacatcttc aacagttgca tttttcacca tattggcaac aggtgctatt ccgtcagccc
    20161 aaggcatatt gatatccaca ttacctaaat taatattttt cacagaacca ttttcaacac
    20221 ggtcaaataa gggacgggca atgttatgaa ttgtatattg tttaccatca acacttgata
    20281 gatgtccctt aaacgtacct ggaacatatt gtttgttagg tgttggtaca ttcgttgcat
    20341 tcaaatctgc tccgatttta aatgtaccat ttttatcagc attcatggca tctactaact
    20401 ctttaaagtt ataataaacg tcatcaactt ttggaactgg tttttcaata taatgaacat
    20461 acttgttacg aaccttcgtt gggtctgtat gttgaatcaa atcttgagct tcggcagtga
    20521 ttttataaag tttcttacct tcttcagtca cttcttcaat tttatcaact gctaaacgtg
    20581 ttactttatt atcacgacta gtaattttta agtacatttt cttcacatct tcatctgaag
    20641 gtttttcagt cataaaatca ctaggtgttt cagtaccatc gtcatttacc ttcactaaat
    20701 tagtagatgc gatattttta atttcaactt ttttcaaatc caagcgaagt ggtttctctt
    20761 ctaatgttgc agtttcttct cccttgccac ggtcataggt catagtggtc tctatcttat
    20821 agtccttgta gaattttaaa tcttctacct gcttatctaa ttgctctgaa gtcaaatcta
    20881 ctgttttgac aatttcattt ccatctttaa ttgtcgcagt aattttattg attgtgactc
    20941 catctgtatt aactaaatga taagaaagag ttgcggatcg gtttaataca tttgtatttg
    21001 ttctatctaa ggtcaaaaca ggagccgttt tttctttagg tttttctttg gtacctttta
    21061 caagaatttt aggcttcatt tctttcaaaa tagttactgt ttcaactggg tcactgattt
    21121 ttttgccatg taactcttca tagctagtaa caatttgacg ttcgccatcc tcaccaactt
    21181 gtctaacaga atcatcgcct ataaatttgc taggatcaga ttcttctata gtggttttag
    21241 gaatagtctc tgtccgaata tcggtatgta aagaaggcaa ctcttctctt tctgatgcaa
    21301 cttctgaaac tgtagatatt ggttcagtat actctggaat gtcgctaaca ggtggctcaa
    21361 tcaagtttcc attttcatcc actcctgttg tacctactgg ctcagtatat tctgggatgt
    21421 cgttaacagg tggttcaatc aagtttccat tttcatctac tcctgttgtc cctactggtt
    21481 cagtgtatgc tagtttctca tgaacttcag gctcaacaag gtttgcacct attggttgcg
    21541 tatagtcagg cttctcatga acttccggtt cgcctttctc tgttacaact ttgggttgct
    21601 caactggcga aacagttgat gctggagaaa ccgacgaaac agttttatca tcaagctcca
    21661 agtaaccaac atatctgtaa ccagaaattt gcaacacacc atcacgtcca gactcatgga
    21721 tgttagcatt caaattaagt gctgaagcag ttgatagtgt aactaaacct gtcgccccta
    21781 caatcaaaaa tgtagctatt tttttatttc tattatcttt tgtgataatc acaataagac
    21841 tcccgatagc taacaaaccc aatgcagtca taatagattg agaacttcct gtgtttggta
    21901 gtgcgtcttt ttcataatcc aaagcatatg actctttgga ttcatcaggt ctaccttgtt
    21961 tgagttgatc acgttcagtt tgtgtcaaac tactataatc taagtaatga taagtcgatg
    22021 caccaacaac tgacggtgca aataaaagac tccccaagaa taccgaacca acaataccct
    22081 ttattttccg aatggaaaat ttatcttttt ttaataatga caattctaat cctccacctt
    22141 cactttttat ttcctaatag taaacactat tttttattct caaaaattga acttctaaaa
    22201 tcaccggttt ggtcaagata agctttaaag attgcttttt tcagtttaag aactctacta
    22261 tttgtctcag gatataattt ggcaacttca tcctcagcat caaattttac tgcatcttca
    22321 atcatttttt caagtctatt aatattatca atagtcactt tctgttgtcg agcccaagaa
    22381 ccgtttggat cgataaaact aactttattt aactttccaa atttatcttc tctttcttta
    22441 tacatatctt tcttgaaatc tatccaagaa ttatactggt tattaaatac ttttgaaaga
    22501 acaatttcat cagtaactaa acctatttgt ttaccatatg atgtaataac ttttccttga
    22561 gctttcgctt ctttttcata ctgattagaa atatatggaa ccataccgtc tttgtacccc
    22621 ttggctgcca ataactcaaa tgcaatacgt cgtcccatta aatctcctgg tgttcctatt
    22681 ttactgctca atgctgaata aattggtgag aatagattaa ttgtatagta gccattccgc
    22741 ttataagttg cttcatctac ataacctctt cttgttataa tatcatgttc tatcaaactg
    22801 ttaaatgtag ttaatttagt aagttcttct ggttttaatc tacgtacaac attcgttgca
    22861 taaacagaat ttccatcagg atcaggttta tattcatttt ctatttttct taataaatca
    22921 tatttttgat ttgtatctaa tttagctaca gctttacctt ctagtatttc aagcatgtat
    22981 attacatcaa acatattatg catatactta tttagatcat caacacttcc aaatcgttga
    23041 gttggatctg ctacttgaag ccttgttgat tcttctgatt gatcatattt taatatggaa
    23101 ttaatagtga ctgttggatc attaggatga tcaggagctt gtagcagtcc tttagcataa
    23161 aactctggtc caagtccatt tcgtcttcca tagcctccaa gataaatttc tctatctgag
    23221 ttatgtgtca tttcgtgtgt ataagttacc gcaccatctt tatcaagcat gcgatatgcc
    23281 atataataga atgcatcccc tgtagcatat gctccattag aattatgaac aacattattt
    23341 cccgctggac cgaagaaatg ttttatagct ggatgattag tttcaaaagt tgcttgatat
    23401 gtatctttat cttctttttc atctgtacca aatctaaatg catcatagac tagaacactt
    23461 ctatataatt tttctttatt tttatcatct agtattctat accaataatc atagtgatct
    23521 ctaaatctaa cagccgcttc tttagctttt ttctcaacat agtcatttaa atcagttcct
    23581 gctggatgtt ctttacttct atatctatca taagcaccga aacctatagt cgaaatgttg
    23641 gctataataa acaccgattt ttcaggtaat gttaatagcg gcaacaccat acttggatat
    23701 ttccaagatg ggctagttaa tctatcatat actccaattg aatatggact accagctatt
    23761 gattgcttat cacttacctc tttaatagta gatttttctt ctacaatata tgctttagtt
    23821 tgttctttaa accactcatt atttgtttta tctggtacaa atgcttttct atatttctct
    23881 aatgcatcaa ataaattatt agtcttataa tttttcgcta ataaaacatt ataagttata
    23941 acattatttt ttgctagtaa gttattatat cccgatttac ctagttcgat aacattatct
    24001 aacgctgaac tatttgattt accgaagaaa tccaagtggt acatgactaa atctttcgct
    24061 tttgtatcac gatatttgaa gtcgtaccaa cgctctaagt atgttaatcc tagaagtaaa
    24121 gcttctttat tatttttaat tttctcgatt acataattat ctattacctt attgttattt
    24181 tcagctattg cagcatctgc agtaagaagt tttgtcaaac tatctttcaa attagttttt
    24241 gtggtattga attgttcttc taaatataac tccgttaaac taacatcctt agaaacatct
    24301 aatacttttc taattgcctc tgatttatat tctacactat ttaatgctgg taatacttca
    24361 tctaaaatat tttgataatt gcgaagtaat gtattcggtg tataaattaa gtttgtaccg
    24421 cctattctat actctgctac atttgaaaaa tcagtttgat aatttacatt aagtgtctct
    24481 gatgtattat ccgcgtaata tagtaatagt ttatttatac ttgatttatt tttgttaata
    24541 tcactaatta cttctttatc tttcataggg actactgaca ataattcttt attataaaga
    24601 ttactacttg tctctactaa atttccgtac ttaacaattg tttctctatt ataaaacggt
    24661 aatagttttt caatattttt atatactttt tcttttgatg aatcagcatt ctcaagattt
    24721 ttatagtcag ttacatatct agaagagaaa ttaaaatctt ctgttttaat agtattttta
    24781 gatacaaaag tagctttttt ctcttttatt tgtgcatctt ctaatacttc tcctctttga
    24841 acagtagaat ccttagttaa gatttcatca tcttcttgta ctacgttcac aaccttatta
    24901 tttttattac tgtatgtgcc atttgctttt ataccattaa aattataacc tgcaatagca
    24961 ttaccattcg ttacattaac atcgcttagt acattagaaa gtcgaccgga tttttctaat
    25021 cctccaaccg gatcccaaag attaccaact actccaccaa cgtttgcaaa acgttgaaca
    25081 ttatttagat ttccttcagc ataactatta ctaataagcg cactattttc tactgcacca
    25141 actattccac caatagactg gtctccttgt gttgcatttg aagataaatc gattgatgcg
    25201 aacgacttat ctattaatcc cctcggacca gaaagtttgc ctactaaacc tccgatttga
    25261 taactagcaa ctgttttata tgtattagtt attctacctg tatagctact gttagaaata
    25321 gtagaattat taacttgtga tactaatcct cctatgctac gttccccagc tatcgctcca
    25381 tcagcatgaa cgttatcaat atgagtattg ttatttgctt ctttcgcaag tgttgcagta
    25441 tcgtcctttg cggaaatatt aacatctttt agtgaaagtt tttctactgt agcaccattt
    25501 aattccccaa ataatggttt tttcaaatta taaatagcat aatatttctc attatttgtt
    25561 cctactaata ttccatggaa tacattattc acataacttt cttgaccatc tggaagttca
    25621 acttcacgag catccatagt tgctcctaaa cggaatgtgc cattaggatt gctattcatt
    25681 gcatctacta aatttttaaa tgatgtatat accccagttt cactttgctg agtttttggt
    25741 aaatagtatg tataattttc tagtacatta ttattttcat gttgtatcag actatttgca
    25801 cttgctacta tcttataaac atctctatta tctttagtat cattagtaat gcttgtaact
    25861 ggtagcatca catctttaaa gttctctgat ttaactttca taaagtaatt ttctgcatta
    25921 cttggtaaag aagcaagtga agttacatgt cgatacttat taccatcttt tgaatatagt
    25981 tctacagaag atatatttct aagttctaat ttcttaataa cttctttttc atctttagcg
    26041 tcatcttttt tctcatcttt atgacgataa agttcatcgt atactggtgg aatttcatta
    26101 cctgctataa tactcttaat ccaagctaat tgtgtaggag ataaaatcaa tccgcctgag
    26161 cgattttgtg ctccattttg atgcacacca ataacttcac cattagcatt aaatattcct
    26221 ccaccactca taccaggatt tccaccttga tattgaacac cgtaggtgtt attcgcatat
    26281 tcttgttcca tttctaccgt agcatctttt aagtgcgtat atttcccagc tggatatcca
    26341 tgaacattta gtttgtcatt aagatttact ttcgaataat ttttaactaa ttctacatgt
    26401 tctctgccag ttgtttcagg tagcttaatc actgctaaat catagataaa tccttttgga
    26461 taatcttttt tattataaaa atgaatattg ttagcagtaa atgttccttt ttgtccatca
    26521 ggagtcaccc actcatatac gtttttagat ttatcacctc taaattcgcc agtagtatta
    26581 tcagtagaat tattaatgaa attatgtgct actgttaaaa gtacatttgg tgcaataaaa
    26641 gtagctgatc ctactccagt aaccttcgac gaatctgtaa taaagttcat attaattaat
    26701 gcactagatt ttttctgaat actcatttct tctggtgtat tagtattttc attatttggt
    26761 ttagtatctt ctgcactagg ttgttcgatt ttaccagtat attctttcag tgcttctact
    26821 ttttctggtt ctactattgc tccggcttgc actcctgtgt attccttcgg tgcttctacc
    26881 ttctctggtt cgactatcgc tccagcctgc actcctgtgt attccttcgg tgcttctact
    26941 ttctctggct cgactatcgc tccggcttgc acccctgtgt attctggtag tggtgctacc
    27001 tgctctggtt caacaatagc gccagcttgt actccagcat attccggttc aactttttca
    27061 ggttcaacaa tagctccagc ttgtactcct gtatattctg gtagcggttc tacctgctct
    27121 ggatcaccct tgtcagtcat gactgcctct ggtaacgctg gttgaacttc tggtacgcct
    27181 ttatcactta ctacagcttc tggcaactct ggttggactg ctgactcacc tttgtcggtt
    27241 aatacagctg ctggtagttc aggctgaatt gcgggttcaa caatagctcc agactgtact
    27301 tccttatgtt ctgcaccagt ctcagcttgt tcctttataa cttgagcttt tttagtacct
    27361 ttttcgacta ttcttgaaac aggcgcagtc gttgaagttg aaataatttc tcgtgaaact
    27421 tcttcattgt ttacagaaaa tattctgacg atttcaactt tcttacctaa tgcaccttct
    27481 tgttttactc ttacagttcc ttctgctaaa tcaggatttt cttgaatttc ttcttgaaaa
    27541 tctatttttg tttctatagt ttcgtcacga tataagagtt ccggtttgtt caactgacct
    27601 gatagcactt catcctgtgg atttaatgta tttaccccag tcttttcctt tggagaaatc
    27661 ttctcctctt tcttcgtttc tagattctta tgttcggcta attgttcttg agaatctgaa
    27721 gattgtttct cttcttttct tggattgatt aattcagtag agaaaggttt ttcaactact
    27781 tgaacttctg tcggcttagt tgaagaaaca ggtgtttgtt cctgaatagc ttgtactgtt
    27841 gatggatggt ctacaaaatt tggtgtaaca ttataatcca ccttttgttg ttttgtagga
    27901 gtggctgctg atttttcttg attacttact tcaaaatcag aagtcgtttt tccctctttg
    27961 atatatccaa tataagtgta acctgaaatc tctttaggaa gaggtaattt ttctccagag
    28021 gtcaattcat agtccgtatt gtaatttagc aaaagatgat tttctaaagc atggactgaa
    28081 actaagacac catttcccat tcccgcaacc aatactaaat gtaataccgt tttattctta
    28141 acctttttct tggaaacagc aaaaattaaa attcccagag cagctaagct agcaccagca
    28201 actagggctt gcctctcatt cttgcttcca gtatttggta attccgccag ttgattttga
    28261 gaatttaact tataaacaag ataataagtt tcattatcat tctccacgta tgtcggaata
    28321 tcatagacaa gctgcttctt ttcttctgat gatagctctg aatctgccac atatttatag
    28381 tgaactcccg cagtttcttg agcatccaca gatgaactag ctaatacaga cataaaaaat
    28441 aaacttgaaa tcgttgcaga tacaagtcct actgataatt ttctaaatga aaaacgctct
    28501 tgtttttcac caaaatactt ttccattatt cctccttgaa ataaaattta tatatgttac
    28561 aaagaccttt attatattag tgtattatct attatctata gaaaaggcag tataccttaa
    28621 ttatactctt aatttacaaa aaagtcttaa aattgagatg cgctttcata ctttgtttta
    28681 tattatttgg gggtacaata acacctacca tgaaatttac acggtaggtg ttactcatat
    28741 cactaatcgt tctaaaaatg gtttgaggca gttgaggaga attccttcta tccagcttcc
    28801 ttgtgctgat gagcgatggt cttcctgcag gctttttttt agaaaatctc ggacttgttc
    28861 tggtgcgatt tcaaattcaa aggctttcat tttatagaaa aagtcgatga gatgatcgga
    28921 caggtattca gttgaaaagg gtacttcacc actttttcga tattctaata agagcctaga
    28981 aaatcgagct ttttcttcag gaagctcacg aaaataggaa ttgaggagcc aagtctgctt
    29041 ctgttttctt tcaattggat cctgactggc aattcgttgg tctttttcca gctctttttg
    29101 gtattgtttg gccttgatag cccgttctgc tctattttta ccaaaaagaa ttttttccca
    29161 cttgcgttct tcttgagtca gggtctctgt aaagccaaag taatcttgat aagcacgctc
    29221 tgcgggtccc atggctagaa ccagattgtc tgcatattgc ttggcgattt tatccctctt
    29281 cttgcgttct ttctctgcct ggatacggag ttcttgttcg tagtcaattt tctccttgcc
    29341 tagcttgaca aggtagagtt ggtcatccga tttcccaagt aaaaagggtt tgatacactt
    29401 ttcaaggact tcttccatcc gagccttttt ctttggttcc gccttggtcc aacttcctcc
    29461 ctgaaagact tctaggaaaa gctggtagtc tctctcaggc gcaaattgat tgccacgatt
    29521 gggtttgaaa acaccttttt cccagagcca ttttagaagt cgctcgtcaa agtcactttt
    29581 attgaccttg attttttctt ttttctgagc ttttctggtt agattttcaa cctttctgag
    29641 cagtttttct tcctcttcca attgctggtc aagggacaat cgatgaaaat gacgaacaca
    29701 gtcgctacca attggaaaga ggcgttggcc tgtgacaccg ttaaagagtt cataagcgta
    29761 tttgatggca tttccacaga cacaattgct acggccgata ccgttaaaaa tcaaggaaac
    29821 ttcattccat tccttggtag cttgttccca agtatccgct ttcgaagcct gtaaaactgc
    29881 atcgtgcagg gattttctaa ctggaagtgt catgaggtct cctttctaat actcaataaa
    29941 aatcaaagag caaactagaa agctagccgc a
//
LOCUS       DAVBNO010000022        27949 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVBNO010000022 DAVBNO010000000
VERSION     DAVBNO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27949
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.022"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(59..1762)
                     /gene="ilvD"
                     /locus_tag="VQ846_001079"
     CDS             complement(59..1762)
                     /gene="ilvD"
                     /locus_tag="VQ846_001079"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET3075663.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(1995..2927)
                     /locus_tag="VQ846_001080"
     CDS             complement(1995..2927)
                     /locus_tag="VQ846_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET3075664.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDVVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSAVCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(2924..3781)
                     /locus_tag="VQ846_001081"
     CDS             complement(2924..3781)
                     /locus_tag="VQ846_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET3075665.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNSGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(3785..5131)
                     /locus_tag="VQ846_001082"
     CDS             complement(3785..5131)
                     /locus_tag="VQ846_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET3075666.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(5144..5428)
                     /locus_tag="VQ846_001083"
     CDS             complement(5144..5428)
                     /locus_tag="VQ846_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET3075667.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(5432..7462)
                     /locus_tag="VQ846_001084"
     CDS             complement(5432..7462)
                     /locus_tag="VQ846_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET3075668.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            7653..8660
                     /locus_tag="VQ846_001085"
     CDS             7653..8660
                     /locus_tag="VQ846_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET3075669.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            8663..8863
                     /locus_tag="VQ846_001086"
     CDS             8663..8863
                     /locus_tag="VQ846_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET3075670.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            9015..9197
                     /gene="rpmF"
                     /locus_tag="VQ846_001087"
     CDS             9015..9197
                     /gene="rpmF"
                     /locus_tag="VQ846_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET3075671.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            9213..9362
                     /gene="rpmG"
                     /locus_tag="VQ846_001088"
     CDS             9213..9362
                     /gene="rpmG"
                     /locus_tag="VQ846_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET3075672.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(9784..11664)
                     /locus_tag="VQ846_001089"
     CDS             complement(9784..11664)
                     /locus_tag="VQ846_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET3075673.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASMIY
                     TT"
     gene            complement(11658..12527)
                     /locus_tag="VQ846_001090"
     CDS             complement(11658..12527)
                     /locus_tag="VQ846_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET3075674.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILYKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(12611..15256)
                     /locus_tag="VQ846_001091"
     CDS             complement(12611..15256)
                     /locus_tag="VQ846_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET3075675.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGHPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAFRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGPLDPQEIRTEFIKKEEI"
     gene            complement(15347..16627)
                     /locus_tag="VQ846_001092"
     CDS             complement(15347..16627)
                     /locus_tag="VQ846_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET3075676.1"
                     /translation="MIYSKEIVREWIDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            16798..18882
                     /locus_tag="VQ846_001093"
     CDS             16798..18882
                     /locus_tag="VQ846_001093"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET3075677.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QGGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGKAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADH
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLNKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(18922..20601)
                     /locus_tag="VQ846_001094"
     CDS             complement(18922..20601)
                     /locus_tag="VQ846_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET3075678.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGSLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIYVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            21357..22586
                     /gene="arcA"
                     /locus_tag="VQ846_001095"
     CDS             21357..22586
                     /gene="arcA"
                     /locus_tag="VQ846_001095"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET3075679.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            22644..23660
                     /gene="argF"
                     /locus_tag="VQ846_001096"
     CDS             22644..23660
                     /gene="argF"
                     /locus_tag="VQ846_001096"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET3075680.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            23825..24772
                     /gene="arcC"
                     /locus_tag="VQ846_001097"
     CDS             23825..24772
                     /gene="arcC"
                     /locus_tag="VQ846_001097"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET3075681.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNSGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            24983..26494
                     /locus_tag="VQ846_001098"
     CDS             24983..26494
                     /locus_tag="VQ846_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET3075682.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            26516..27847
                     /locus_tag="VQ846_001099"
     CDS             26516..27847
                     /locus_tag="VQ846_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET3075683.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQANMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKETGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEICIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT         8235 a         6218 c         5365 g         8131 t
ORIGIN      
        1 aaccatacct acggcaaggc gacgttgacg tgatttgaag agattttcga gtatgagttt
       61 attttttacc tgacttgtcc atattccaga agtctgtcac ggctccgcgt gaagcagatg
      121 atacgatgtg ggcatattta ccgaggacac cacggctgta aagtggtggc aaggttgttt
      181 ctgccttgcg tttttcaagt tcttcttcgg atacggccat ggaaatttct ttggtatctt
      241 ggtcaaccgt aacgatatcg ccggtacgga gataggcaat tggtccacca tcctgagctt
      301 caggagcgat atgtccaaca accagaccat aagtaccacc agagaaacgt ccgtccgtca
      361 agagggccac cttatctccc tgacctttac caacaatcat tgaagaaagt gacagcatct
      421 caggcatacc aggaccacct ttaggtccaa caaaacgaac aacgactaca tcgccatcaa
      481 cgatttcatc tgtcagaacg gcctgaatcg catcttcttc tgagtcaaag accttagctg
      541 gcccaacgtg acgacgcact ttaacacctg ataccttggc aactgcaccg tcaggagcaa
      601 ggttcccgtt caagatgata agcggaccat ccgcacgttt tggattttca agtggcatga
      661 taactttttg gcctggagtc aagtctgcaa agtcagccaa gttttcagct acagtcttac
      721 cagtacatgt gatgcgatct ccgtgaagga aaccatttgc caacaagtac ttcataaccg
      781 cagggacacc accgacttcg tagaggtctt ggaagacata ctgaccagat ggtttcaagt
      841 cggccaagtg aggcacacgt tcttgaatcg tattgaagtc ctcaagtgac aagtcaacat
      901 ttgcggcatg ggcaatggcg agcaagtgaa gagtggcgtt tgtagaacca ccgagagcca
      961 tcgttacagt gatagcatct tcaaaggctt cacgagtcaa gatatctgat ggtttgagac
     1021 caagttccaa catcttaaca acagcacgtc ctgctgcttc gatatcttct ttcttatcag
     1081 ctgattcagc tgggtgagag gatgaccctg gcaaactcat ccctagaact tcgatagcag
     1141 ttgccatggt attagcagta tacataccac cacaaccacc agggccaggg caggcattac
     1201 attcaagacg tttcacgtcc tcagctgtca tgtcaccgtg gttccatttt ccgatacctt
     1261 caaagacaga aaccaagtcg atatctttac catcaagatt tcccggtgca atagttccac
     1321 cataggcgaa aatggctggg atatccatgt tggcaatagc aatcatagat ccaggcatgt
     1381 tcttgtcaca gccaccgata gcgacaaagg catcgacgtt gtgaccactc atagccgcct
     1441 cgatggagtc cgcgataatg tcacgagatg ttagagagaa acgcatacca ggcgttccca
     1501 tagcgatccc gtctgctacg gtaatggttc caaactgtac aggccaagcg cctgcagatt
     1561 tgacaccttc tttagccagt ttcccgaaat catgcaagtg aatgttacat ggtgtatttt
     1621 ctgcccaagt cgaaatcact cccacaatcg gtgtttcaaa gtccttatct gtcataccag
     1681 tcgcacgaag catagcacgg ttaggtgatt taaccatgct gtcataaatg ctactgcggt
     1741 gacgtttatc taattcagtc atcttatccc tcccatttca gtttttacta ttatagcaca
     1801 atttaaaggc aatgaacaga ataaaattct tgaattttca gaaaattcta tacgcatgtg
     1861 aaatatttaa aattaaaaac aacaaagcgg attagtgcac tttctgatga ccagaatatg
     1921 ctttttaatc cgctttcttt aaataacgta ctgtaatttt tacagaaatt ctttcaaata
     1981 agtgtattta acatctatct tgcattataa atttctagaa ccttctcttt tatattcgat
     2041 tcactcaaac catactcatt aagaagataa tccattttcc ctacttgacc aaatctttct
     2101 tgaacaccca tccgatgaat ttttgttatt ccatcatcag agaataattc acatacagca
     2161 ctgccaattc cacctatctg attgtggttt tctacagtaa atatagtttt tccacttaac
     2221 attgttttta tctgttctgg tatcggtttg attctaaata aatctatcac acctactgaa
     2281 taacctaatt tagacagttc atctgcaact cgaatacttg gagcaaccat tatgccagaa
     2341 gcaacgatta caacatcttc accatgcctt aactcaatgt agcctttaga aaaatcttct
     2401 ccaccttgat acacaggaac tggagctttt ctaattgttc gaatatattt tagtcctttt
     2461 aagtctaatg tctggttcaa tatttcacga aattggatat catcagttgc ttcaaaaatg
     2521 atcgatttag gaattaaacg taacaatcca atttcttcaa atggcatatg tgtcccacca
     2581 ttcatttctg cagttactcc tgcatctgat ccaatcacag tggcatccaa ttgtgcgtat
     2641 ccaagagaaa taaataattg atcaaatact cttcgtgaag caaaaggacc aaatgtatga
     2701 agataaggtc taaacccctg aatagacaag cctgctgcaa ggccgaccat ttctgcttcc
     2761 ataatcccaa cattcacata acggtctcca aagtcctttt caagattatg agtagccatc
     2821 gaacttgaca aatcggcttc taagactact atatcagaat cactttgatt agcctctaaa
     2881 aggaagtctc tatatacatg ccgtaattct ttcgtacttc tcatcattct gtttcctcca
     2941 attcctgact taatctttct acaactgaag ttaacatttg tttctcctct acagtagggc
     3001 gaagatgatg attggatttc atttcttcca gctcttgaac cccttgacct ttaatagtat
     3061 ctaatacaat gcacttaggt gatgaattat ttgactgttt taattggaca atcccttcat
     3121 aaatttctct aatatctgaa cctttgaccc taatggattc aaatccaaat gctgaaaatt
     3181 tttctacgaa atcacctgaa ttacaaatat cctttgtaaa accatctaat tgttttttgt
     3241 tatcatcaac aaatacaatt aagttggata actgttgatg agaagcaaac tgtatagcct
     3301 cccaacattg tccctcattt aactcaccat ctccaacaat agcgtaagta taaaagggac
     3361 tctttcttat tctctgacca tatgcaagtc cagttgcaac actaattcct tgtcctaaag
     3421 agcccgttgt catatctatg cctggcgtta gatttctatc aggatgagac ggtaatttgg
     3481 ttccatttgt atttaaagaa tataagaatt ctttgtcaaa gaaaccattc aaatagagtg
     3541 tactgtatag agctggtcct ccatgacctt ttgataatat gaaataatct ctatctcgtg
     3601 ctgcaaatat ttctggagtc attggcatta tttcaccata aagcactgct aacacttcta
     3661 cgatagacaa acttcctccg taatgtccga atccaagatg attcaatgtt ctaagagtat
     3721 ttaatcggat gttagtcgca aattttctta acccatcttc tctattttta cttaaaatca
     3781 tcccttattc ctcctttgca gatggctttt taataaagga tactccaaac ataactgcta
     3841 aaataagaac aagaccaatc acaatgcctg cttgtgagcc aaattgattt aacattccta
     3901 aaataattcc tgatagacca aaatctgcat ctgagaaagt tgatccttgg aaaccaagtc
     3961 ctcccaaaac tggcattaaa aagactggaa gaaaactgat taaaatacct tgtaaaaatg
     4021 ctccaatagt ggctccacga acaccaccag atgcattccc aatgacacct gcagtcgctc
     4081 cacagaagaa atgaggcaca acgcctggta agataacaac cgttcctgaa gcaatcataa
     4141 ttgccatact tactaaacca ccaacaaaac tagagataaa tccaattaga actgcattgg
     4201 gtgcataagt ataaacaatc ggacaatcca aagcaggttt tgaattaggt acaagacgct
     4261 ctgaaatacc tttaaaggct ggaacaattt cgcccaaaat aaggcgaaca cctgctaaaa
     4321 taacaaatac ccctgctgca aattgacctg ctaattgtaa agcataaact agaccacttg
     4381 taccactact gatttctttt tctatatatt ctgaccctgc aaagatagct acaataatgt
     4441 aaataactgc catggataaa gtgatactaa cagtactatc acgtaaaaaa gctaaactct
     4501 ttggaaattt aatgtcctct gttgattttg atttgtcacc gataaggcta ccagtaaaac
     4561 cactcaacca atatcccaaa gaactgaaat gacctaaagc taccttgtca tttccagtta
     4621 attgaaccat atatttttgc acaaatgctg gggaaatact cataataata ccgagtgcta
     4681 atcctcctag taagataaga ggcaagctag taaagccagc aactgataaa atgaccgcaa
     4741 tcatacatgc catatataga gtgtggtgcc ctgttaaaaa aatatattta aatcgagtaa
     4801 aacgagcgat taagatattg aacaccatgc ctgcaaacat aatcattgca gtagctgagc
     4861 catatgttgt taaagctaca gctacaattg cttcattatt cggcacaacg ccagataaat
     4921 gaaaagcatg ctcaaacatg gtaccaaatg gatttaaaga attttgtaca attcctgcac
     4981 caccagatac aactaagaaa ccaacaaagg tcttaattcc acctttaata atatcaggta
     5041 atttcttctt ctgaagaact aatcctaaaa ttgcaattaa agctactaaa atagctggtg
     5101 tactaacaat atccaatatg aacttcatca tgacgctagc ctcctatata agtccttttt
     5161 cttcacaaag tttagtaatt aattctcgta gttcatccat atcaataata ctatttaaga
     5221 tacgaacatc tccaagatga ctagctgaat cagctagatc acgaccaaca atccaaatat
     5281 cagctgcatt tggatctgct ccacctaaat cataatgttc aacttctaca tccgaaacat
     5341 tcaaatcact caatacagat tcaatattca tctgtaccat aaaacttgaa cctaatcctg
     5401 aaccacaagc tgtaccaatt tttaacatta tctaatcctc ctgtttaatt atcattttaa
     5461 tgtcatcata gttttttgat gatattaaag tttgaacatg atttttatct cttaaaattg
     5521 ttgttaaatg tgacaaagcc tttaaatgac tctcattatc aatggctgca atacaaatca
     5581 acaatcttac ctcttgttct ggattatcca ataaataaat cggttcttcc aaaactaaca
     5641 ttgacattcc tatttcattc acaccttcat ctggccgagc gtgaggaatt gctactccct
     5701 tccctaaatt aataaaaggt ccaaactctt ctactttttg aatcattgcc tcagggtagt
     5761 tctcagttat cttatcttga tccaaaagcg gtttagctgc taaacgaatc gcctccttcc
     5821 atcctaattt ttgcgaacta acctgatagg tttctttggt aataagttgt tctagcactg
     5881 gtacaatttc ctttctatca tttttttgat aaagataatt ctttaacgcc aatcttaatt
     5941 ccaattcttg tgtaataatt ccatatcttt tgacaatatt caggatttgt tcaatctcaa
     6001 aatctccata ctctaaattc ggaaaatctt ttaacactag ttctactagt tgtattgctt
     6061 gctcttcagt catcataacc gaaactagat aatttggctt ttctgtctcc acctttatgg
     6121 tagaaaaaac catatcatag tcactactag ctttcacctg taaatcatca atctttgagg
     6181 ttcctataaa ctcaatttga ggaaataatg ctaatagatt ctcttttaac atcaatgaag
     6241 aactaacacc attaggacaa atgattgctg ctttatagca tttttgaggc aaagtatctg
     6301 ctttctttaa ataacctccg aaatggataa caaaatatgc tgtttcacta tcaggtatgg
     6361 gattgtcaat agcgtccatc aagggcatca aagaatcttt gactaattca aataaatcag
     6421 gataatgttc tttaacatgc aatacatatt catttgaact aggtaggccg aactttaatc
     6481 tatagtaagc cggtataagg tggcggcgaa gattttctct caatccttcc ctttgtttaa
     6541 aatgtaacaa agaaatatct tccattctac ttataatagc ctctgttaat tgattaaagt
     6601 aaaccggagc aacatctact tcaccttcaa agcaacttga taataaaact gtgatatagc
     6661 gataatcatc ctcagaaaac accgtatcta taattcccaa atcaaccact gtatccaata
     6721 aaatagtggt tatatcttga ataacaggag atactaatgt ctctgaaaga catactcttt
     6781 caacatccct ttgataccta cacagaatga atactaaacc gaaaaggcaa acttttaatt
     6841 gattaacaat aggtactagc tgtagcttct cataataatc tttaactacc tgatttatca
     6901 aatcataagt taatgaatac ccccaactgg ataaaacata atccaaaccc caaatcccta
     6961 tggaggattc cagcaactca ctaaccattt gaaaagctaa gcggtgctta ttccactctg
     7021 aaccgtgtaa agtataacct tttgctctac tgtaccctag ctccaaatca ttatctaaca
     7081 taatctttct taatgattga atatcagata aggttgtatt cttacttact ttcaaaaagt
     7141 cttggtaatg attattcgat ataaaatcta atcggcaaaa agtgtaaaga tagattaaag
     7201 ctaagcgagt cgactttggt aaaaccaatt catccgactt aataatatct gtcaaagact
     7261 gcttcgtacg atttgataaa ctatagcgac cttgcttttt atccagcact atccctttat
     7321 tagctagata aggcactaaa taatctattc cttctttgac ttcctttata ggtaagctca
     7381 ccttaacaga taattcatat aatgatagct cacaatgatc catcaaagtc atcaaaataa
     7441 ctagtgctct ataatcaaac atttctcttc ctttctaact acaattatag tgtaaaagtt
     7501 ataagaataa aagagtattt gagcacaata taatctcacc ttttaaaaga aattattggg
     7561 ctgaaagttt ttagtgatta ttttcaactt tctattgact tttaaaaaca aagtgatatc
     7621 atttagatat caaataatgg gaggtcattc ttatgactga aaaactaatc aattcaaaac
     7681 caaatggtgt attcgcattg attctcattg agttgacaat cgtacttggt atctttatat
     7741 ttataatggg tgttggttcg gaaaacattt ttggaattat tatcggacct ttactaatcg
     7801 taattgcagg tctagctcat gctggtttaa aagttgtcaa acctcaggaa gctctggttc
     7861 tgacactctt tggtaattat acaggtacca tcaaagaacc tggtttttac tttgtcaatc
     7921 ccttcagcgt agcagtcaac cctgcaaacc acactcgact tggacaaagt ggtgatgtta
     7981 gcacaaaatc tcctttttta ggagctaaat catcaaatga caatgatgta aatcttgaaa
     8041 ttggcaagaa acacatttcc ctcaaagtca tgaccttgag caattctcgt caaaaaatca
     8101 atgattgctt aggaaaccct gtagaaatcg gtatcgcggt aacttggaga gttgtcgata
     8161 ccgctaaggc agtcttcaac gttgataact acaaagaata tctttcattg caatgtgata
     8221 gcgccctccg taatattgtc cgcatctatc cttacgatgt gtctcctaat gtggatacta
     8281 cgggtgacgg gcaagcagat gaaggtagtc tccgtggctc tagcgaaatt gttgctaacc
     8341 gtattcgtga ggaaattcaa agtcgcgtag aagatgctgg cttggaaatc cttgaagcac
     8401 gtatcactta cctagcttat gctccagaaa ttgctgccgt tatgcttcaa cgccaacaag
     8461 catctgccat tattgatgca cggaagatga ttgtagatgg tgctgtagga atggttgaaa
     8521 tggcactaga acggctcaat gaaggggaat tggtagaact tgacgaagaa cgaaaagctg
     8581 ccatggtttc aaatctcctt gtcgttcttt gtggcaatca tgatgcacaa ccaattgtca
     8641 acacaggaag tctttactaa agatggctga agctaaaaaa aagcagatcc cccttcgact
     8701 ctcaacaaag ttatacgctg cactcgcatc atgggcagaa gacgactttc gttcagtcaa
     8761 tgggcaaatc gaatatctcc tcacagagtg tgtcaagcaa cggaaaaaag acggaaaata
     8821 cgtatcagaa accattgacg aaccatttga gattgatatt taacaccttc tcctgtctgc
     8881 cgtgtcggat aggagaattt tttcatcttt ttttgtcaag taactttact ttacaaaaaa
     8941 aatgtgttat cctagtatgg ttgatgaaaa tcagtagatt gaatcgaata taaataccta
     9001 aaggagaaat caaaatggca gtacctgcac gtcgcacttc aaaagcgaag aaaaacaaac
     9061 gtcgtacaca ctacaaagta acagctccat ctgtaaactt tgacgaaact actggagatt
     9121 actcacgttc tcaccgtgta tcacttaaag gatactacaa aggacgtaaa atcgctaaag
     9181 ctgcatcagc tgaataatag aagggagata ccatgcgcgt aaatattaca cttgaacaca
     9241 aagaatctgg tgaacgcttg taccttactt ctaaaaacaa acgtaacact ccagaccgcc
     9301 ttcaattgaa gaaatactca ccaaaacttc gcaaacacgt tgtgtttaca gaagtgaagt
     9361 aagcattcaa tacgaatcaa tacagaagaa aaacgcttga tcagcgtttt ttctttttgt
     9421 taatttcact atattgcaag ggaaatcatt cgaatgacta cattattgat aatatttgtt
     9481 aagccatcaa aagtaattaa catttcatag cgagtaatcc tatttaaact cgtcccacta
     9541 tctatttctt cttatctctg tgttataatc ccgactttat cataagtttg tctaactgta
     9601 gcatttgaaa ccagataatt cttctgtttc acgcgaaaca ccctgcgcca agcaaggtgt
     9661 tcttgttttt atactcaatg aaaatcaaag agcaaactag gaagctagct gcaggctgct
     9721 caaaacactg ttttgaggtt gtagatggaa gctgacgtgg tttgaagaga ttttcgaaga
     9781 gtattaagtc gtataaatca tcgaagctgt cgcaatggta tgccactggt tagctgtagt
     9841 tgctgcgaaa tccttgtctg cgtagaagtc agtaaatggt aggatttcaa cttccagctc
     9901 gtcgatgcgg tcaagctggc cagccagata aacctcgata cggctttctg ctcgtttgat
     9961 gcgttgcaag agtccgccca tacggatgtc aactgtatcc aaaccaaaga ccttgttttc
    10021 tttcagccat tggtgactaa agagggcatg gaagtcttca atttggcttc taagttctgg
    10081 taattcttgt ctggcgattt gttgtaaact ttctttatca tccgcttggt aggcctgacg
    10141 aatgcgtcgt cccacatcta ctttgctact taaaatagca ttcaactggg cctgagtttc
    10201 aaagagataa gcatagtttc cagctttttc tttaatgtta gcaagcgtct cagcagcctg
    10261 agcgaagtgt ggcttgtcct gttcaggtgt catgtgtcgg tcaagtatcg gacagagaac
    10321 atcctgataa aagacatagc ggttgggatt gatgccactg agattacctg gtaggtctgg
    10381 taagaggttg gcaagatcaa tctgcataaa atcctcaacc gttagaccag tattggtctt
    10441 gaaatgcgca gacaaactat ctaggtcatt gcgatagctg agttctgccc agatttgcaa
    10501 gcttggtagg atagagaact gggcagtttc accaccattg tctccccaac ccgttacgat
    10561 gacttcttta atctgattgg cacggcaggc tttattagcc tcgatagcca ctagacggct
    10621 aaaatggttg tgaggtgtaa agccaatcca cttccaagct ccccctgcaa atgcaaggtc
    10681 atggctaatc ttgtgatgat tgcggaaatt acggttgtat ttttcctcgc tatcctgata
    10741 ataatcccag taaaccagag tcacacggtc tttgagacgg tctaggtaga cacgagtttc
    10801 ctctggaatt tccacatcac ggtcgtactg gccatccgct gacatgagtt tgaagaacat
    10861 atcactccac atctggcagt ggaaaccata tttgtcagca atatccagca cgcgctccaa
    10921 gtgttggcac atgaggagac tacgatccac aacaccgttc aaaatcaggt agcgtcccaa
    10981 accaaccaag tgggcttcgt ccatcccgat attgaccttg cgagtcttca gtttagacaa
    11041 cgtggcaaac atgccatcaa tcaggtcata aaccttttct tcgccaatga gaagaatgtc
    11101 ctctacatca cggagctcct gcacttcctt gacaccccat ttgacaaagg ccgacaagtg
    11161 ggccaaggtc tggatgcatg gtacaaaggt catgtcaaac tgttgggcat aggcttcgat
    11221 ttcctgcaac tcctctgctg aataagctcc acggaaatag ccaaagtaag gctgcccttc
    11281 aatctggtaa gtgtcttcca tgtaaagctc aaaggttgag tagcccatga gagccaagac
    11341 ctcaatcatc tgcttggcag aagccacatt cagcaccgca tttcgagaac agtcaaccat
    11401 gtaagccaaa tcttcgtaag ctgcttgttc ctcaatctct actttatcag cttctgctag
    11461 aactgttacc aacaaggata aggcacgata aagttggtga ggtttgcggt aggtcaattg
    11521 atagtgacct tcctcaccct cgatagagat agaggcttgg tcagactgag tgacagccac
    11581 ttccacatct ggtagagaaa tgtgaccttt taaaacctca atagcttgcg tttgtttggg
    11641 actaagtcct gtaaatctta ccattgcttt tcctcctgta gccagttgac aagagcaccg
    11701 tagagattgg catctgcgtg ataggtgcag gcctggataa ctggtgcgac cgtgtattct
    11761 tcgtaggtat cgacaaagtt atcaacagcc ttcttgacat cttggataaa atctggattt
    11821 tgactgatag agccacccag actgatgaca tctggatcga tgagatactg gatattgagc
    11881 aagccttgcg ccagattgcg gttcatgcgc tcaatggctt cttgacaaag agcattacca
    11941 gctgcggcct cttggtaaat cttgcgaccg tcccaatcag tatgaccaga tttttcaatc
    12001 acgtatcgta ccatattccc agttgatgct agttgcgacc agttattaag tttttcagca
    12061 ggggcaaggg ttgtcatgta gccaaattct ccacccagac cgtggcgacc tcgatgaagt
    12121 ctaccattga taatcatggc tccgccaatc cctgtcccaa tcacgacaca ggctgcattt
    12181 tcaagctctg gatgagctag tagttcactg agtccaacgc agttggcatc attttctaaa
    12241 tggacaggta gctgataaga gctaagcgcc tcataccaag aaaagccatg gatgtagggc
    12301 accgcactga agccatcaat cacacctgtc tcttgattga ccgcacctgg aacgctcata
    12361 gcaatcccac tgtaatcctg ttctgacaag cgttgatcta gccacgctag taaatcctcc
    12421 aagttttcag gcgttgaaat acttgtctta tacagtattt tcccatcagg agtcagactg
    12481 gcaaacttaa tcccagtccc tccgatatca atcgttgcaa tggtcattgt ttttaccata
    12541 aaaaggggcg aatcagcagt tagttcttac tttttcgccc ctcctttctt ttgatgttta
    12601 ctttttgaaa ttaaatttct tcttttttga tgaattctgt acgaatctct tgtggatcca
    12661 atggtcctga atgaactgga tatggtcgct caagtaagtc aagaatggtt tgttgatgtt
    12721 ctgatatgcg cacattttct tgactcatat tatagtaacg gaagacatag ccttcttcat
    12781 tttccgccac tttaaaggct gttggacaaa cttgcggtat gctgagaaca gaatggctca
    12841 agaggctacc agtcgcaacc acgcttcctt cctgtctagc aagctgaagg ctggtaaacg
    12901 gtgtctgcaa ggctttggca cgacggaaag ctgaaaagcg ttcttgggct tggtggcatt
    12961 caagtgcaaa ctcgacttca aactcacgca agcattgtgc ttctggcgtt gggaagtagc
    13021 cccagtcacc tagctcacct gatgcacgca aaatggtcac ggcaatggtg ttatccccaa
    13081 ggatttcgta ttcattcaat cccttattgg atacagtcac ccctttttca tcgtcataca
    13141 gactgacaaa agcttgttgg tgttgaggat tttcaggatt ttcccatgaa gcagctggtt
    13201 tgtttggtcg tgtcaccacc tcatagatac tttcagaatc attgcttgga cgcgtgttat
    13261 gagtcttgac caagagacgg atacggtgat ccttggcagt gttagtaaag cgagtcttga
    13321 agcggatttg tggattgtca acgaagacag tcaactcagt ttccagagga atgtttgtca
    13381 attcttctga ccgtccagcc tcacgcttca taaactcgat gataccttgt tgctcttctt
    13441 ctagcttttc atccgcactg acaggcacgg tcaattcatg tttgagcaag attttagcat
    13501 agcgatctgt gttttccaag acctcgtggc ccttaagctc tgcaaagatt ggctctgttc
    13561 cttttggttg gaaatagata tactcgtttc cgatgtcccc acggtcttcg aagcggataa
    13621 agtcttcata ggcttcgtga gttgtcttgt catatactgt gatgttgtca tccacactca
    13681 ccgttacgaa tggtgtatca atcactccgt tttggtaaat accctcacgg tgttcttgtt
    13741 ttccttccag caattggaag gttgtccaag aaagcggcgc taggtgaact ggaatggtca
    13801 cgcgcacttg tcgagcaata cgagcttggc ggaacttgtc ttttggtaaa tcgtactcaa
    13861 aattagctcc gaggtcttcg attgtagcct ctacaggatg accatccaag tcctccacac
    13921 ggtaacttgg caagataaga gcagccatct ttttgtagcc ttctgttggg tgcaattcct
    13981 tgaaatcaca agtcgccaca tcaatcactg tgctgacagt atcgacctta tcatgcaagc
    14041 ctgtgttaat gacagtaaag agatagtcac tttgagcctt atccgtagca attttaccct
    14101 tccactcgtt gagcaaatta cttttaacaa agtttcctac ttggttgacc ttggcaaaac
    14161 gcgtttccat ctcgcggtga acttcgtcca cgctacagcc acagatacta tcatgtggcg
    14221 cattctgcaa aagtgttttc caagcatagg tcaactggtc cttgtggttg tgtccaccag
    14281 tgataatagt caagggttct accacttgct ctaggaggtt gctattttct tggaaggctt
    14341 gttttaggta aatgcgggat gaagaagtgt tggcaagtgt gtaccagcca tctgtttcct
    14401 gactggtcaa ctcgcctgta acagttgata agtgttcagg aagcgcacct tctacagctt
    14461 gaacatattc atcaaaagaa ctatgaacaa agattacatc tgggaagagt tcatttgcca
    14521 cacgaatggc ttcgctcaga tttttctgta caggctggtg gtcacagccg ttcatcatca
    14581 accattggtt ggtcgaagcg taggcacgca catctgacaa tttttgtttc cagaaggtca
    14641 aggcctcatc tttgtcaact gggatttcat tcccattact gtaccagtta gcaaagagaa
    14701 tacctaaaac acgactacca tccacaccct gccagtacat ttctgaaaac tgggatgtaa
    14761 actgctcatc ttcaaggact tggttgtcaa atccaatcgg cttcacacca cgaccaaagg
    14821 ccgccacgtg aatgcctgat ttttgaagaa tttgaggcgc ttgtcccata tttccaaagg
    14881 tatctggaaa gtagccaatc tgggttgatt taccccattt ggcagcttct tgttgaccaa
    14941 tcaaggtatt gcggacattg gcttcactgg agatcaagta gtcatcctgc aagatgtaaa
    15001 agggaccaat tttaagtttg ccctcgtcaa tgtagcgttg gaccttgtcg cgattttcag
    15061 ggcgaatttg taagtagtca tcaaggacaa tagtttgtcc atccaagtgg aaactcttga
    15121 actcagggtc attttcaaag agatcaaaga gattgtcaaa caattccacc aactgcatac
    15181 gatggctttc aaaaggcaag taccactcac ggtcccagtg actatgtgag ataatatgta
    15241 caacaacatt ttccatgaag taaaacctca ttctaaattt aaattttaac gttttaaatg
    15301 taaacttgtg attctgataa gaatcggttg agctaaagcg agccccttag cgaatatcca
    15361 agtaatccaa gaccaactca cagaacatca tgttagccca ggagaaccat tcacgagagt
    15421 agagggtcgg atcatctaca tgaaagcttt cgtgcatgac acctgtacca ccatcgcagg
    15481 caaccagctg atccagcaag aattttttct ctgccttatc tcttgttgtc aatccttgga
    15541 tagaaagggc gattggccag atatagcgat agaaggtatg agaacttccg agaccgctag
    15601 cgtattctcc ttggtagaaa tatggatttt cagagctcaa aatggtacga cgagtagctt
    15661 gatacacttc atcatcgacc gaacagtagc ccagataggg cgcagctagt agacttggta
    15721 catttggatc atccatgatg ctggcatttc ctaggccatc cacttcaaaa gcgtaaatct
    15781 tttcgccctt gctgttggtg gtgtaagcgt agtttttgat tccttcttgg atttcatcct
    15841 gaagacgctt ggcatcagca ataacactct ggctatcagc taggtttaat gctgcgaaga
    15901 tttcttgcac ataacccaag actactacag caaacatatt tgacggaatc aagtaactat
    15961 actgacagca gtcatcgctc ggacgaaagg ctgaccaagt catacctgtc actgcaaagt
    16021 caggtccaaa gccatcattt accaaggtgt cttccttacg gtccgtgtct cggacaaaac
    16081 gataaggaga gttcttgtgg tcttgttcca ccgtccacag atggagaatt tccttagtcg
    16141 ctgcgacaaa aatctcatca aactgactag tctcgccagt ctctttccag aggagataag
    16201 ccaactgcaa aggatagcaa agcgaatcca cctcatactt gcgctcccag atccagccgt
    16261 taaggtctgt gtggtcagtc tcgtggtgcc ctttccagtt ctcctcaatg ttgaaggagt
    16321 tagcataggg atccttgagt accaaggtca tctgacgttt gaccaaacct gcaatggtct
    16381 gacgcaggag ggcatctctt ttagctacat gaaggtaggg tctgagttgg gctgtcgaat
    16441 ctcgaagcca catggcagga atatccccag tcaagacaaa agttgaacca tcttctaaga
    16501 tttcaaccgt attgtccaag gtatcggtgt agcaacgctc gaaaacatcc acccactctg
    16561 gatggtcctt agcccgctct gctacttcat ctatccattc tctaacaatt tctttcgaat
    16621 aaatcatcgt gcaattccct ctttcaaaca agtttcatgt tcagtatata gcttttgaga
    16681 cagaaaatac atacacaaat gatagtcatt tttctacaaa atagttattt ttgaaactcc
    16741 ttttctaaag gccctctttt tctgatataa tgtagaaaaa cagctaaagg aaagactatg
    16801 aaaccactac ttgaaaccat cgatacccgc tttggtactg ctagcaagca tgccttctct
    16861 cggggaaata ccctgccata cacaggcgtg ccttttggga tgaattactt tgtgccccag
    16921 accagtgacc agggtggatc ttggtttttt gatccgcatc tgcctatctt tcaggggatt
    16981 cgattaactc accagcccag tccttggatt ggggactact cttggctcct tctgacacct
    17041 gtcaccagtc aactgggggg agacagcctc tttcaccgcc agtcttctta tgatatagat
    17101 aaagcttgct tccagcctca ctatttgaag ctcttttctc tgcgttatca gattgaaacc
    17161 cagctcacac cgacttgcta tggtgcttct attcgtttga atcaaaagca aggcaaagcc
    17221 ctctccctct atcttcacgc agcagatgaa ctgacagtag agcaagtaga taagcggact
    17281 cttgccctgc gacaagaagg taaaactgaa actaacaaaa attcgctaac gatgttcact
    17341 gccctgcaaa tgaacacgga tattcttgct atcagccaag aagctggaga ctggcgaatt
    17401 gacttagcaa gtagtcaaac cgagatgcaa ctagccactt ctttcatctc tccttctcaa
    17461 gctctaatca atctacctca agaagatttt gatagctgta aaacaagtgc ccaagcggat
    17521 tgggaaaatc tcctccatcg ttttgacatt atagagacag gagaggctga ccgaaccttc
    17581 tttgaccact gtctctacag actcttcctc ttcccacaga ctttttatga ggttgatgag
    17641 tcagggaaag ccatccacat ggatctggct actggtactg tcaagcctgg tgtcctcttt
    17701 agcaacaatg gtttctggga taccttccgc accaccttcc ccctctttgc ccttatcata
    17761 ccggagcact atcaacgctt tttagaaggt ttcctcaata gctaccgcga tactggtttc
    17821 cttccaaaat ggctggctcc agatgaacgg ggtatgatgc caggtacact gctagatggc
    17881 attatcgcag atagcgcctg taaggacatg gcccccgacc tagaaggaga actcttccaa
    17941 gccatgctcg aaacagccag caaagccgac catctcggca tcaatggccg ccacggacta
    18001 gcccaatacc aagaactagg ttacctctct accgaccacc atgaaagtgt tagccatact
    18061 ctagactatg cctatagtga cttttgtatc gccagctgtg ccaaaaaact agagaacata
    18121 gaaatcgctg aaacctacaa ggctgcttca caaaattacc gccagctatt tgacgctgag
    18181 acaggttaca tgcgagcacg agacaatcaa ggaaactttc accctgactt ctctccttat
    18241 agttgggggc gagactacgc tgaatgctct gccattcaag ctactttagg cgttctccac
    18301 gacatccctg gcttaatcca actgatgggt ggaaaagaaa cctttagcaa ctatcttttg
    18361 aaagcctgtc aagatgctcc cctctttgaa acaacaggct atggttatga aattcacgaa
    18421 atgagcgaga tggctactgc tccttttgga caaatagcca tttccaacca gcctagtttc
    18481 cacatccctt atctcttccg ctacagcgat taccctgact acactgccct tcttatcaag
    18541 acgctccgtc agaaagcttt tcacccaagt tgggaagcct atcctggaga tgaagacaat
    18601 ggtagtctct ctgcttggta catctggtca gctctcggat tttatccaac ctgtccagga
    18661 aaaccaagct atgacctcgg aatccctctc tttgaccatc tacgagtcta cctagctaaa
    18721 gaagataaat ggctggatat ccatactaaa caaaaccaca accattttaa ctttgtcaaa
    18781 gaatgccgac tgaacaaaac actcgtatca actattcaac accaagacct cttaaaagct
    18841 gaacaactaa ctttcaccct cagctggcta ccaagtcact aataaccaaa agaacctttg
    18901 catcgcgcaa aggttcttct tttatgaaac ttggacttga agttgatcca caagctgacc
    18961 atccaccgtt aggttcagat agaaatccaa ggttttatct cctgcgaaat acagggttgg
    19021 tagatgcaaa tcttttttgc tttcccctgc ttggaattga aggacataaa tctcgtcctg
    19081 ataggcgaca ccatggacac ctgtccccgg ttgaatcgaa atcttagctt ctaaaggacc
    19141 actacattct ctgcgcttaa ctgtaaaata ggcattttct ccctttgcca cagctaggct
    19201 tttttctgca aatgctagtt cctgaacaac ttcttttttt gataatccag gagttttata
    19261 aagggaaatc ttggtcaata caggaataga ttgtgcctcg gtaatcatca cgcgcacctt
    19321 ttgtgcctct actagcgaac ctcgcaagag gcgcttatga ccaactgtga agcccctgcc
    19381 aaattcctgc cagacaccat ccacctctac ttgcacatgg aaagcagaaa tacgttggcc
    19441 taacttcaaa tcttctctca actcaattac atcaaaagtt ttaggagaac ctaagtcgag
    19501 ttctaactgg atgggcaagt ctgcatcgtt tgcccaagag ctggtctcaa ggccgtctgt
    19561 caaatggcga caagcaaagt ctgcggaaag agctggacca gatacctcag ctcccagagc
    19621 caaatcttct ttatagagct cattgcgata ggtcgcaaat tcataaagtc gttcaatatc
    19681 ctttgcatca aagagcccag cttgattcgg cggaatatta agcaagagtg gagttcctcg
    19741 ccctactgag tgaaagtaga tttcgaccaa ctcctcgaga gacttaggat cctgatcctc
    19801 atggtagaac cagcctggac ggatggaaac atctgcctct ccgattgaaa aaatcgtgcc
    19861 cgagggatcc ccgtgctgaa gatagttcag ctctgcttct gttcctagtt tatcaggatt
    19921 caccttttgc cacagtggat cacctgcata ccctcgttca ttgccaatcc agcggatact
    19981 ggtgccttct gttgaaaaaa tcaagcaatc gccctgcagg tcacgaatgg tttcaaacca
    20041 tttttcaaat tcataattaa ccttttgcgc gccctctcct ctggcaccat ccatccaaac
    20101 ctcagcgaac ttaccagcat tcccatagtt aggatttgat aagatttcct tcaactgagc
    20161 cagataatag gcattgtagt ccgcttctcg gtccacatga tagaggggac tatgggcatc
    20221 ccacggtgac aggtagaccc ccatatccat atcaaactct gtggcagctt gggatacttc
    20281 aaggagcaag tcgccctttc ctctcctcca aggactgacc ttaaccgaat aatctgtgtg
    20341 agctgtcgga taaaggacaa agccatcgtg gtgcttgacc accaaaatca actttttgaa
    20401 gcccgtttcc ttgagcacac gaacccactc acgcgcatcc aactgactcg ggttaaagcg
    20461 ctcaggatcc tcctgtccag tcccccattc ttggtcataa aaggtattag gaccgaagtg
    20521 gataaaagct gctagttcat ctcccagata agctagctga gtctgacttg gtaacggtcc
    20581 atgcggtttg attttcttca tcgttatata tctccttctt tagcaacttc tcttgttcac
    20641 aaggttatcc acaggttgtg gataataaac catgatctaa cagccagctc tttccaactc
    20701 caactggtag gctcaggatt ttgctcctca atattccctc ttcaaacagc tctgtcatat
    20761 ggttctgcta acacctctca gaatgctact ctccataatt attctcaggc ttctttcagg
    20821 tcattgtgtg aagaccattc actctccaga cttttccaca gattgtgaat aagtgattat
    20881 tctaaactat cacctcgtcg actataacaa aaaataaaaa gagggtcaat aagataatta
    20941 tctcttccta aatactcaga aaagattcta taatttgtac aattgaacaa gaaatgcttc
    21001 tccaaaatct atcttcaaaa gaatgttgca tctttttatc ctttcacaca aacatttctc
    21061 tttatacaga tccaagtgat aaccagatga taattggaca ctttttggtt atttgtgtat
    21121 tttttgaaaa attttttaaa aaaattttat atcctcttta atagaagttt atgaatttgt
    21181 atatatgtat tccatcctat gttatgtata ataacataaa attgtgctaa atatcataat
    21241 ttatattatt ttccgagaaa ttatagccat tgacattacc taggaattgc gttataacat
    21301 acatgtaagc ggtacccaaa atggtgccaa gtcaaaattt ttaaggagga aaatacatgt
    21361 cttcacatcc aattcaggtc ttctcagaaa ttgggaaact gaaaaaagtt atgttgcacc
    21421 gtccaggcaa ggagttagaa aacttgttgc cggactatct tgaaaggctt ctttttgatg
    21481 atattccttt cttggaagat gctcaaaaag aacatgatgc atttgcccaa gctcttcgcg
    21541 atgaaggaat tgaggttctc tacctagaac aactcgctgc tgaatcattg acctctccag
    21601 aaatccgcga tcaatttatc gaggaatact tagacgaagc caacatccgt gatcgtcaaa
    21661 ctaaggttgc tattcgtgaa ttgcttcacg gcatcaagga caaccaagaa ttggttgaaa
    21721 aaacaatggc tgggattcaa aaagttgaat tgccagaaat tcctgacgaa gctaaagatc
    21781 taactgactt agttgaatca gagtatccat ttgcaattga cccgatgcca aacctctatt
    21841 tcactcgcga cccatttgca acaattggaa acgccgtatc gcttaaccac atgtttgcag
    21901 acactcgtaa ccgtgaaaca ctctacggta agtatatctt caaataccac ccaatctatg
    21961 gcggaaaagt ggatttggtc tacaaccgtg aagaagatac gcgtatcgaa ggtggagacg
    22021 agctagttct ttctaaagac gtccttgcag taggtatctc tcaacgtaca gacgcagctt
    22081 ctatcgaaaa acttttggtc aacatcttca agaaaaatgt tggcttcaag aaagttttgg
    22141 cctttgaatt tgctaacaac cgtaaattca tgcacttgga tactgtcttc actatggtag
    22201 actatgacaa gttcactatt cacccagaaa tcgaaggcga ccttcacgtt tactcagtta
    22261 cttacgaaaa cgaaaaactt aaaattgttg aagagaaagg tgacttagct gaacttcttg
    22321 ctcaaaacct tggtgtagaa aaagttcatt tgattcgttg cggtggtggc aatatcgtag
    22381 cagctgcgcg tgaacaatgg aacgacggtt ctaacacttt gaccatcgca cctggtgtgg
    22441 tagttgttta tgaccgcaat accgtgacca ataagatttt ggaagaatac gggcttcgct
    22501 tgattaagat tcgcggaagt gaattggttc ggggccgtgg tggacctcgt tgtatgtcta
    22561 tgccatttga acgtgaagaa gtgtaatcgc tgttcggtat tcgtcaatag aaaatgtaaa
    22621 aaatagaaag aggaaataat aaaatgacaa attcagtatt ccaaggacgc agcttcttag
    22681 cagaaaaaga ctttacccgt gcagagttag aataccttat tggtctttca gctcacttga
    22741 aagatttgaa aaaacgcaat attcaacacc actaccttgc tggcaagaat atcgctctcc
    22801 tatttgaaaa aacatctact cgtactcgtg cagcctttac aactgcggct atcgaccttg
    22861 gtgctcaccc agaatacctc ggagcaaatg atattcagtt gggtaaaaaa gaatctactg
    22921 aagatactgc taaagtattg ggacgtatgt ttgacgggat tgaattccgc ggattcagcc
    22981 aacgtatggt tgaagaattg gcagaattct caggcgttcc agtatggaac ggtctaactg
    23041 acgaatggca cccaactcaa atgctcgctg actacttgac tgttcaagaa aacttcggtc
    23101 gcttggaagg cttgacattg gtatactgtg gtgatggacg taacaacgtt gccaacagct
    23161 tgctcgtaac aggtgctatc cttggtgtca atgttcacat cttctcacca aaagaactct
    23221 tcccagaaaa agaaatcgtt gaattggcag aaggatttgc taaagaaagt ggcgcacatg
    23281 ttctcatcac tgaagatgct gatgaagcag ttaaagatgc agacgttctt tacacagacg
    23341 tttgggtatc aatgggtgaa gaagacaaat tcgcagaacg tgtagctctt cttaaacctt
    23401 accaagtcaa tatggactta gttaaaaaag caggcaatga aaacttgatc ttcctacact
    23461 gcttgccagc attccacgat actcacactg tttatggtaa agacgttgct gaaaaatttg
    23521 gtgtagaaga aatggaagta acagacgaag tcttccgcag caagtacgct cgccacttcg
    23581 atcaagcaga aaaccgtatg cacactatca aagctgttat ggctgctaca cttggtaacc
    23641 tttatattcc taaagtataa ttttagataa taaaccgtct accaacagct atgagggctg
    23701 cgactaatag ctttagtccg gtcctctttt atataatggt aatctattat ttcttataaa
    23761 atatgtgaaa aatcattaaa ttgaaatcta aacgcattct attgagtgtg ataaaggaga
    23821 atttatggca aatcgtaaaa ttgtagtagc tttgggagga aatgcgattc tttcttctga
    23881 cccatcagca aaggctcaac aagaagcttt agttgaaaca gctaagcatc ttgtaaaatt
    23941 gattaaaaat ggagatgatt tgattatcac tcacggtaat ggacctcaag ttgggaatct
    24001 cttgctccaa catttggcat cagactctga aaagaaccct gccttcccac tcgactcact
    24061 tgtcgctatg acagaaggta gcatcggttt ctggttgaaa aatgctttgc aaaatgctct
    24121 cttggatgaa ggcatcgaaa aaaatgttgc ctctgttgta acgcaagttg tcgtagataa
    24181 aaatgatcca gcttttgtta acttgagtaa accaatcggt cctttctatt cagaagaaga
    24241 agcaaaagca gaagccgaaa agagcggagc gactttcaag gaagatgctg gccgtggctg
    24301 gcgtaaggtc gttgcctcac caaaacctgt tgacatcaaa gaaattgaaa ccatccgtac
    24361 tcttttaaat agtggtcaag tcgtcgtagc tgcaggtggt ggcggtattc ccgtcgtcaa
    24421 agaaaacaat ggacatttga ctggtgtcga agcggttatt gataaagact tcgcttccca
    24481 acgtttggca gaattggttg atgcagacct cttcatcgtt ttgacaggtg tagattatgt
    24541 atttgttaac tacaacaagc caaaccagga aaaattggaa catgtgaatg ttgcccagct
    24601 ggaagaatat atcaaacaag atcagtttgc accaggtagc atgcttccaa aagtagaagc
    24661 agctatcgct tttgtcaatg gtcgtccaga aggaaaagca gttattactt cccttgaaaa
    24721 tctaggcgcc ttgattgaat ctgaaagtgg aacaattatt gaaaaaggat aagttgtttt
    24781 actaataaga tgtattctat ttctagtatc tttatatcaa attagaaatt attcttgaaa
    24841 acatgtacaa tatttcaaaa gatattagtt ttttagactt taatatggta aaacaaatat
    24901 aaatagaaag cgttttctta aatgtttatt taagaaagta gttggttttt tacactttgt
    24961 tagacatcag gaggaaaaac aaatgagtga aaaagctaaa aaagggttta agatgccttc
    25021 atcttacacc gtattattga taatcattgc tattatggca gtactaactt ggtttatccc
    25081 tgcgggggcc tttatagaag gtatttacga gactcagcct caaaatccac aagggatttg
    25141 ggatgtcctc atggcaccga ttcgggctat gctaggtact catccagagg aaggttcgct
    25201 cattaaagaa acgagcgcag cgattgatgt agccttcttc atccttatgg ttggtggttt
    25261 ccttggcatt gtcaacaaaa ctggtgctct tgacgtaggg attgcctcta tcgtgaagaa
    25321 gtataagggc cgcgaaaaaa tgttaatttt ggtactaatg cctttgtttg ccctcggtgg
    25381 tacaacttat ggtatgggtg aagaaacaat ggccttctat ccactccttg tgccagttat
    25441 gatggccgtt ggttttgata gcctgactgg tgttgcaatt attttgctcg gttctcaaat
    25501 cggctgtttg gcatctactc tgaatccatt tgcgacaggt attgcttcag cgactgcggg
    25561 agttggtaca ggggacggta tcgtacttcg tctgatcttc tgggttacct tgactgctct
    25621 tagtacttgg tttgtttacc gttatgcgga taagattcaa aaagatccga ctaagtcact
    25681 ggtttatagt actcgcaaag aagatttgaa acactttaac gtagaagaat cttcatctgt
    25741 agaatctaca cttagcagca aacaaaaatc agttctcttc ttatttgtgt tgacattcat
    25801 cttgatggta ttgagcttca ttccatggac agaccttggc gttaccattt ttgatgactt
    25861 taatgcttgg ttgactggtc ttccagttat tggtaatatt gtcggttcat ctacttctgc
    25921 actaggtact tggtacttcc cagaaggcgc catgctcttt gcctttatgg gtatcctgat
    25981 tggtgttatt tatggtctta aagaagataa gattatctct tccttcatga atggtgctgc
    26041 tgacttgctc agtgttgcct tgatcgtagc gattgctcgt ggtattcaag ttatcatgaa
    26101 cgacggtatg attaccgata caatcctcaa ctggggtaaa gaaggcttga gcggtctatc
    26161 ttcacaagtc tttatcgttg taacttatat cttctatcta cctatgtcat tcttgatccc
    26221 atcttcatct ggtcttgcca gcgcaactat gggtatcatg gctccacttg gagaatttgt
    26281 aaatgtccgt cctagcttga ttatcactgc ttaccaatct gcttcaggtg tcttgaactt
    26341 gattgcacca acatctggta ttgtgatggg agctcttgca cttggacgta tcaacattgg
    26401 tacttggtgg aaattcatgg gcaaactcgt agtcgctatt attgtagtga ccatcgccct
    26461 tcttctcctt ggaaccttcc ttccattcct ataaaatagt gagtgaggtg attccatgaa
    26521 aatagatata acaaatcaag ttaaagatga atttcttata tcattaaaaa ccttgatttc
    26581 ctatccttca gtactcaatg aaggagaaaa tggaacacct tttggacaag caatccaaga
    26641 tgtcctagaa aaaactttag agatttgtcg agacataggt ttcactacct atcttgaccc
    26701 taaaggttat tacggatatg cagaaatcgg tcagggagca gagcttctgg ccattctctg
    26761 tcatttggat gttgttccat caggtgatga agcagattgg cagacaccgc catttgaagc
    26821 aactatcaaa gacggctggg tattcggacg tggtgtccaa gatgataaag gcccttcgct
    26881 cgcagctctc tatgcagtaa aaagcttgct ggaccaaggt attcagttca aaaagcgcgt
    26941 acgctttatc tttggtaccg atgaggaaac cctctggcgc tgcatggcac gctacaatac
    27001 catcgaagaa caggccaaca tgggctttgc acctgactca tcttttcctc tgacctatgc
    27061 tgaaaaaggg cttctacagg tcaaacttca tggccctgga tcggatcaac tagagcttga
    27121 agtaggaggc gcctttaacg ttgtaccaga caaggccaac taccaaggtc ccctctatga
    27181 acaggtttgt aacggtctca aagaaactgg ttatgattac caatccactg aacaaaccgt
    27241 aacggttctc ggagtgccaa agcatgctaa ggatgctagt caaggtatca atgctgtcat
    27301 ccgactagct accattcttg ctcctctcca agaacaccct gctctcagtt ttcttgcaac
    27361 acaagcaggt caagacggca caggaagaca aatctttggt gatatagcag atgaaccttc
    27421 tggtcaccta tcctttaatg tcgcaggtct catgatcaat catgaacgtt ctgaaatctg
    27481 tattgacatt cggactcctg tcttagctga taaggaagaa ctagtagagt tgcttacaag
    27541 atgtgcacaa aactaccaac tccgctacga agagtttgac tatctagcgc ctctatacgt
    27601 cgcaaaagac agtaaactcg ttagcacact gatgcaaatc taccaagaaa agactggcga
    27661 taacagtcct gctatttcat ccggtggtgc cacttttgct cgcaccatgc caaattgtgt
    27721 agccttcggc gccttattcc caggagcgaa gcagacagaa catcaggcaa atgaatgtgc
    27781 cgttctagaa gatttgtacc gtgctatgga tatttatgcc gaagccgtct atcgacttgc
    27841 aacttaatca ggcaactgtt tctaccaaaa aaaatcgacc gattaatgaa ctgcacccca
    27901 aaagttagac agaataaatc taacttttgg ggtgttttat tatgaaatt
//
LOCUS       DAVBNO010000023        27475 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVBNO010000023 DAVBNO010000000
VERSION     DAVBNO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27475
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.023"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(1..505)
                     /locus_tag="VQ846_001100"
                     /pseudo
     CDS             complement(1..505)
                     /locus_tag="VQ846_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(581..1357)
                     /locus_tag="VQ846_001101"
                     /pseudo
     CDS             complement(581..1357)
                     /locus_tag="VQ846_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1281..1475)
                     /locus_tag="VQ846_001102"
     CDS             complement(1281..1475)
                     /locus_tag="VQ846_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3075684.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLSLFEEEQNMEEDSDLPS"
     gene            complement(1645..1995)
                     /gene="tnpB"
                     /locus_tag="VQ846_001103"
                     /pseudo
     CDS             complement(1645..1995)
                     /gene="tnpB"
                     /locus_tag="VQ846_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1976..2161)
                     /locus_tag="VQ846_001104"
     CDS             complement(1976..2161)
                     /locus_tag="VQ846_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075685.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2465..4027)
                     /gene="guaA"
                     /locus_tag="VQ846_001105"
     CDS             complement(2465..4027)
                     /gene="guaA"
                     /locus_tag="VQ846_001105"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET3075686.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIRDIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4169..4867
                     /locus_tag="VQ846_001106"
     CDS             4169..4867
                     /locus_tag="VQ846_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET3075687.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERHVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4910..5806)
                     /locus_tag="VQ846_001107"
     CDS             complement(4910..5806)
                     /locus_tag="VQ846_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET3075688.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSAFTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5815..6750)
                     /locus_tag="VQ846_001108"
     CDS             complement(5815..6750)
                     /locus_tag="VQ846_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET3075689.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGSVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLSQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6747..7472)
                     /locus_tag="VQ846_001109"
     CDS             complement(6747..7472)
                     /locus_tag="VQ846_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET3075690.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLVSISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(7556..8191)
                     /locus_tag="VQ846_001110"
     CDS             complement(7556..8191)
                     /locus_tag="VQ846_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET3075691.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(8193..9020)
                     /locus_tag="VQ846_001111"
     CDS             complement(8193..9020)
                     /locus_tag="VQ846_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET3075692.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(9042..9377)
                     /locus_tag="VQ846_001112"
     CDS             complement(9042..9377)
                     /locus_tag="VQ846_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HET3075693.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(9378..9909)
                     /locus_tag="VQ846_001113"
                     /pseudo
     CDS             complement(9378..9909)
                     /locus_tag="VQ846_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            10540..11151
                     /locus_tag="VQ846_001114"
     CDS             10540..11151
                     /locus_tag="VQ846_001114"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET3075694.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11196..11924
                     /gene="rlmB"
                     /locus_tag="VQ846_001115"
     CDS             11196..11924
                     /gene="rlmB"
                     /locus_tag="VQ846_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET3075695.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEITEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(11963..12874)
                     /gene="trxB"
                     /locus_tag="VQ846_001116"
     CDS             complement(11963..12874)
                     /gene="trxB"
                     /locus_tag="VQ846_001116"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET3075696.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12940..13164)
                     /locus_tag="VQ846_001117"
     CDS             complement(12940..13164)
                     /locus_tag="VQ846_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET3075697.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13242..13985)
                     /locus_tag="VQ846_001118"
     CDS             complement(13242..13985)
                     /locus_tag="VQ846_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET3075698.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(13985..14785)
                     /locus_tag="VQ846_001119"
     CDS             complement(13985..14785)
                     /locus_tag="VQ846_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET3075699.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14943..15299)
                     /locus_tag="VQ846_001120"
     CDS             complement(14943..15299)
                     /locus_tag="VQ846_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET3075700.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15312..15824)
                     /locus_tag="VQ846_001121"
     CDS             complement(15312..15824)
                     /locus_tag="VQ846_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET3075701.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(15824..16318)
                     /locus_tag="VQ846_001122"
     CDS             complement(15824..16318)
                     /locus_tag="VQ846_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3075702.1"
                     /translation="MEIRLAFPNEVDAIMQVIEDAKKCLADAGSNQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16453..16899
                     /locus_tag="VQ846_001123"
     CDS             16453..16899
                     /locus_tag="VQ846_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET3075703.1"
                     /translation="MKTTFSYPKWAEIPNINLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16896..17543
                     /locus_tag="VQ846_001124"
     CDS             16896..17543
                     /locus_tag="VQ846_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET3075704.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(17564..18145)
                     /gene="pdxT"
                     /locus_tag="VQ846_001125"
     CDS             complement(17564..18145)
                     /gene="pdxT"
                     /locus_tag="VQ846_001125"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET3075705.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18146..19021)
                     /gene="pdxS"
                     /locus_tag="VQ846_001126"
     CDS             complement(18146..19021)
                     /gene="pdxS"
                     /locus_tag="VQ846_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET3075706.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19173..20552)
                     /locus_tag="VQ846_001127"
     CDS             complement(19173..20552)
                     /locus_tag="VQ846_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET3075707.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20846..21769
                     /locus_tag="VQ846_001128"
     CDS             20846..21769
                     /locus_tag="VQ846_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET3075708.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21829..22434
                     /locus_tag="VQ846_001129"
     CDS             21829..22434
                     /locus_tag="VQ846_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET3075709.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFSDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22453..23697
                     /locus_tag="VQ846_001130"
     CDS             22453..23697
                     /locus_tag="VQ846_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET3075710.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(23920..24177)
                     /locus_tag="VQ846_001131"
     CDS             complement(23920..24177)
                     /locus_tag="VQ846_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET3075711.1"
                     /translation="MDPKKIARINELAKKKKTEGLTSEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(24219..26255)
                     /locus_tag="VQ846_001132"
     CDS             complement(24219..26255)
                     /locus_tag="VQ846_001132"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET3075712.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEYFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(26515..27432)
                     /gene="glyQ"
                     /locus_tag="VQ846_001133"
     CDS             complement(26515..27432)
                     /gene="glyQ"
                     /locus_tag="VQ846_001133"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET3075713.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEILLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
BASE COUNT         8098 a         6166 c         5009 g         8202 t
ORIGIN      
        1 ataatagctc ttgctttagc tccttcaaaa ctttgagaaa ataaccaccc tcgctttttt
       61 aaactgagct caggctaaaa gagtccactg gactctttta ctccgtccca taaccaatga
      121 tttaatggcg cgttcagcta gattattgga aaggaccaga tgtccgtctt ttaaaatggt
      181 cttaaaggtt tctttatact tgaggctgta ttcaattgcc cttcctagtt ttgaacctgc
      241 taaaactgac tgacggcggc accaagcaaa gaagtcttcc attaggggct ggacgtttct
      301 gtagtcgttc atcagctggc aaagcctccc agtctctttc caaggcaaat aactggtcac
      361 gataggctaa acctttagct cctaaggatg atttatctgc ttgcttgggg gtcgcttcaa
      421 aaaatttcct tctcacatgc gcccaacatc caacaagttc agcttcttcc agttgacgat
      481 aagcttctag cggttcgcag tttaccaagc ttagcgtcgc cttgctccta aaccttagac
      541 tgctatcgca taggcagaac tagaggacta aagttctaag ttactgccgc aacatatcac
      601 aatgcatata gccagaataa tttcctagga attcttgcac taccgaacca ctccgacgct
      661 gatcatggtg gtacagcgta atcccttgtt tctcagcttt cccagacaaa aaagtccaat
      721 agtaagtcag ctgactatca ctttctaaga cccgataaga ggtttcatct gcatgaagaa
      781 gagcttgttc taacaacttt tctcgtaaaa ggttataaag ggactctaaa tagtattgac
      841 tcgccttgat atgccaatta gcaatttcct tacgtgtgat tggtaaaccc atcttagccc
      901 aatcttcttc ttagcgataa ttgggtacct tcagaataaa cttctgatgg atggtgtgag
      961 cgataataga agctgagcca aggctatgcg ccaaaggggc tttaggaata ggagctttca
     1021 cgattttatc acttggatta ttcttactgc atgtttggca tttgtaagcg tgttggatat
     1081 gattcactct ttttaattgc gcaagaataa agaccaattc ttgtcgttga aggctcgctc
     1141 caatctcttt tagctctccc tgacagtcag ggcaaatgca gttctctaag cgatgatgaa
     1201 cttcttctga atcaaattgg gcaagaatag cttgacgttt ccctttagct ttcttacgtt
     1261 tataggtgat ttcttctctt tcaactgggt aagtcagagt cttcttccat attttgttcc
     1321 tcttcaaaaa gactgagttg tccagatggg caaacacttt tctcagagga ttttccatag
     1381 agcttttgcg ttagataagc cacttgttca cgaagaaggg caagttcatt ggtgagacta
     1441 tcaattgtag cactctgttg ttgaatgatt ttcaatcttc tcccatagac tttcctcctt
     1501 atttcctaac tttattatat agtgtattga atctataaca gtacaccttg actgctaaaa
     1561 tatttctata aattaatttg actttcctga tagagatgtt catatcttat ttcaattcac
     1621 tatactaaaa agaaagtcct catttcaata gaaatcacga ctttctgatg aatttatttt
     1681 tggagtgata gaaaagccct tcataagcca gtctacttgt tcaggtgtga gagctttgac
     1741 atccttttct gtactgggtc aagtcagtct gccgttctca aagcgtttat atagtagcca
     1801 aaatccttga ccatcccagt aaagggcttt aaagcggtct ttacgtccac cacaaaagag
     1861 aaaaacttga ccggagaaag gatctaattc aaagtgggtt ttaaccagat aagccagtga
     1921 atcaatgcct tgcctcatat ccgttttccc acatacgaga tagacctgcc ctaggctaga
     1981 tagatgaatt gtcatagagc aacaccttat ccaaaagctg ttctaccatt tcgagattga
     2041 gagattgaaa aaaacttact tcaagcttgc caatacgaat tttaagcaga atatcatttc
     2101 tctttttaga atcaaaagga cgagattgtg gaatctctac aggaacaata ggttgtggca
     2161 ttaaaatatc ctccaatagt tttactttac taatagtata caggaggagc tccatgaatg
     2221 atagatacct tgttatgacg cgcttaccat attagaaagt tggctctcta attaaattaa
     2281 ttctcattat atatatcaaa agaccgcagt ttgaaaacta cggtctaatt ttttgtccaa
     2341 atgtaaacca cttggaacta ttattcaatt tattcttttt agcaaatgtt cccgttttaa
     2401 tcccagttac agtttttaat cctttgtaac cgttgatttt acaaagcttt taattttttt
     2461 gcgattactc ccactcaact gttgcaggtg gtttacttgt aatatcgtag acgatacggt
     2521 taacatgatc cacttcattt acgatacgta ctgagatttt ttgaagtact tcccatggaa
     2581 ttttggcaaa atcagcagtc ataccatcga tagaagtgat agcacggatt gcaatcgtgt
     2641 agtcatacgt acgaccgtca cccataacac cgactgaacg aacgcctgtg ttaacagtga
     2701 agtattgcca aatatcgcgg tcaagtccag ctttagcgat ttcttcacga agaatagcgt
     2761 ctgattcacg aacggtttca agtttctctt cagtgatttc acccatgaca cggatagcaa
     2821 gtcctggtcc tgggaatggt tggcgccata cgatatggtc tggcatacca agctctgtac
     2881 caagagcacg aacttcatcc ttgtaaagag tattgagtgg ttcaatcaat tcaaactgca
     2941 tatcttctgg aagaccaccc acgttgtggt gtgacttgat agtttgagct gtatccgtac
     3001 cagactcgat aacatctgta tataaagtac cttgagcaag gaatttcaca tctttgagct
     3061 tgcttgcttc gtcatcgaat acatagacaa actcgttacc gatgatttta cgtttttgtt
     3121 caggatcaga aacgccagca agtttgtcaa ggaaacgttt agcagcgtct gctttgacga
     3181 tattcaaacc aaacttacca ccgagcatgt ccataacttg atcagcttcg cctttacgaa
     3241 gaagaccgtg gtccacgaag atacagatca attgatcgcc aatcgctttt tggagaagaa
     3301 ccccaacgac agatgagtca acaccacctg atagaccaag aaggacacgt ttatcaccga
     3361 cggtttcacg aatttttttg atctgcatgt caatgaaatt atccattgac cagtcacctt
     3421 tagccttaca aatgttaagg gcaaagttac gaaggatatc atttccgtat acagaatgac
     3481 gaacttctgg gtggaattgg ataccgtaaa tgtgtttatc tgggttttcg atggctgcgt
     3541 atgggcagtc agctgatgta cctgtacgaa caaagtcagc aggaatctca gtaaccgcat
     3601 caccatggct catcaaaaca gtctgttcat caggtgttga ttcaaaaagc gctgatggtg
     3661 tgtgagttag ggttgattga ccgtattcac gatttccagc atcacctgca ggaacaactt
     3721 ttcctccaag tttatgggtc aataactgca taccataaca gattcccaaa attggaattc
     3781 cgagttcgaa gatttctggg tcaatatcaa atgaaccatc ttcatataca gaatttggac
     3841 cacctgatag aataattcct acaggattga cttcacgaac ttcagcagct gaaattttat
     3901 ggctttttag ttctgaaaaa acaccaatat cacggatacg gcgtgaaatc agctggttgt
     3961 actggctacc atagtccaat acgatgattt tttctacatc ttgcaaatca gttgaaatgt
     4021 tgctcatctt tttcctttct caaaagaaat atcttttctc aatattctat cacaaataag
     4081 ggcgaattac caactaaaca aggcgaagtt tgactttttc tagtttattg gggtacaata
     4141 gagaaaagat agaaatgaag tgaaaaatat gctacctgct tatatgaaaa tccatgatca
     4201 gataaaaaag gatattgacg agcaccgttg ggctattggt gagaggcttc ccagtgaaag
     4261 agatttagct gagcagtttg cggtcagtcg catgaccctc cgccaagccg tatctctatt
     4321 agtcgaagaa ggcgtcttag agcgccatgt aggaagcggc acctttgttt ccagtactcg
     4381 agtacaagaa aagatgcgag ggacaaccag ttttactgaa attgtcaaat cccaaggtaa
     4441 agttccctct agccagctca tttcctacaa aaaaaccatt cccaatgagc aagaagttgc
     4501 caagctagga atttttccaa cggacaatat tatccgaatg gagcgggtcc gctatgccga
     4561 ccaagttccc ctagtttatg aagttgcttc tattcctgaa aaattcatta aggactttaa
     4621 aaaagaagaa atcaccagtc atttcttcca aaccttgcaa aaacatggct atcgtatcgg
     4681 caaatctcaa cagaccatct atgctcgcct tgctaaagaa aagattgccc actatttgga
     4741 agttgaaaaa ggacatgcta ttcttggatt gacccaggtt tcctacctag aagatggtac
     4801 tgcttttgaa tacgtaaaaa gtcaatatgt aggcgaacgc tttgaatttt atcttgaaaa
     4861 taattagatt accacgcaaa aactctctgt cacggacaaa gagtcttttt tatttttcta
     4921 agaataaatc tttcaaagaa atcaaagtta cagcaaacaa tatcattgcc ataccaagaa
     4981 aatcaatggg ataaaattgt tcattcatta ttaagaaggc aaagaaaatc gccgatattg
     5041 gctcaattga agccaacaag cttgacttga ccggtcctat cagactggct cctttaagga
     5101 aagctgtata ggcaaagaca gtcccgataa ggataatgcc tgcaaacgca aggagaaaat
     5161 caagactagt cgggatatcg gcctgtagaa cccctgtaaa aggaagagcg accaaacctg
     5221 ctatgaccat tcccacacca atgaccaagc tgctccccca ctttttaatc aaggctatgg
     5281 gtaaaatgat atacagagca taagtaaagg cagaaaagag accccagaac agaccagcag
     5341 gtgtcatgga taactggtcc aactgcccat gtgttgcgat caggaaggtt cctccgatgg
     5401 cgaatatgat ggaaactatc tctcccagtg tcggtgccac cctatccttg atacagctat
     5461 aaattaaaat tccgacagga caaacatact gaagcaccgt cgctgttccc gcattggtct
     5521 cctgaatagc agacagatag gcgaattggt tgagaaaaag accaatcaga gcaaaaataa
     5581 gaagagacag caaactcttt ctatccttta aaaagaccag tattttatcc tttgcagtag
     5641 cataagccaa gagcatgaga attccaccag cgattaaaag acgcaagttg gtcaagacca
     5701 gagccgaaat tccgtgtgcc attaggtatt ggccactcgt tcctgacaac ccccaagcaa
     5761 taccagccac aactgttagt aaagtccctt ttaaactatt tgacatgtct ctccttactc
     5821 ttctttgcga atcaagttca tcacttgatt gcggtctaat atccattgag ctctctcgtc
     5881 cttttcatag gtacgattcg ataggaaaat agtggcttct tgcttctgac gattccacat
     5941 gataaaggta cctgtatagc ccgtatggtc tagccaatct ccttccaaat tccatgctaa
     6001 agaacgttcc ttgtcatcca aaggagaaaa attttgactt aagtctcttg caaaatcatc
     6061 tgctaaatag tgttctaaaa agatttgtaa atcctttata gtcgaaaata aaccagcact
     6121 cccagcatgt ctacccagga gacgagcctt gggatcatgc actatgcctg cctctacacc
     6181 tctaactgtt ggaacagcaa gctcaactga cccaaacttg gtttccgtca ttccccaagg
     6241 tttccagact tgatccttta aaatcacatc caaatcttga ttaaaaattc tttccaaaat
     6301 aaagcccaac agcaaaaaat ggacatccga ataaagaaag gctggctgac ttcgtctgtt
     6361 gagatgaaac atcgcttcct ttaattcagg ggctgttaaa agatcacgat taggaataaa
     6421 aggatcaagg tctgttgcat gagtcaagag ctggcgaata gtgatgtctg gataatcact
     6481 ctcaggtaaa aaatctatta ccagtctatc aatatctaat tgacctattt cccacaagaa
     6541 ggtacaaact gtgccaaccc caacaacctt gctgacacta gctaggtcat aaactagtcc
     6601 tgcctcagtc tgcaagccat gctctgggtc actctggcct aaatagaact ctgtccattg
     6661 attgtcctta aaatacgcaa aagaggctcc gggataaatt cctgcctcga tttgttcttc
     6721 tattttttta ataatcttgg tccacttcat acttcttcaa accacaattc aacattattt
     6781 ctatctttac aaaggaagaa tttttcagac ttaggaataa aatattcagt agactcaaac
     6841 ttctggcgaa gacttgctat gtctaattca ttgaccaaga acttgagcat agataagtcc
     6901 caggtaaccg tattgtccac agtcaaatcc tgcccctgag ctggaataaa atcaagggat
     6961 gccccaattt cagatgattc caagaaactt tcgatatcag ttgggagatg taattccata
     7021 gaaatctcaa atttacttaa agaaattgat accaaatctg tttgaaattc aggcttttct
     7081 cctacttcta ctagacttgc tctgtcatct tccgcatgaa tcaaaatcaa atcatcttct
     7141 ggtgagaaaa tttcaaaagc gtagccattt tgacctttat ataatcgatg aatcgaatct
     7201 gttttagata agattccttc aatttctaag ggattttcca ccttgacaat caatctagct
     7261 agtttttttc ttccctctac cttacgagta cgcatactgg gagcttcttc taaaaccagc
     7321 ttttcaagac ccgtttggtc acctagtgac agaaaggccg attcttctaa caaggccttc
     7381 attccaaggg tttcaatata aaatgtttca tttaattttc tattattagc ttttaaagta
     7441 ggaataatcc gtacaatctg atttacattc ataaattcct ccgtcacttt ttattttaaa
     7501 ggattttaga attttttaca agatattatg ctcaaattta acattattta gctatttagt
     7561 aaagatagtc tttcaaggct tttgacaaag cctgataacc agcaatactg aggtgcagtc
     7621 catcagttgt atattctttt ttgagttggc ctgcttggtc tgtcaaacaa tcaaatactg
     7681 gcacaaattc cacctgcata taggcagatg caagctcttg ataggcttga ttccagttct
     7741 gaattttttc attcgagcgg atatagactg cctgctggta ctcctctccc tcattgacag
     7801 gcaaaatgga aagcaattta atctctgtca atggataatc gcgagcaacg gattgaatga
     7861 tagcttcgag attattgaga gcctcattca caggaacatc ctttccgata tcatttgtcc
     7921 caatcagaag aaaaatttta tctactgctc caccatatag atgagcatca aggttctcta
     7981 gtaacagtcc tgtctgatag ccacgaattc ctcgattgac aatcgtcttt gaagtcccaa
     8041 atagctcctg tagaggataa tactcgacaa tggaatcccc aataaaaaga atgtttggtt
     8101 ctacaacaga aatgtgattt aggtgacgat acttagtttg aattttttct tgttccttta
     8161 ggagccaatt ttctaataac tgtactgcca cttcattctc ctttttctaa ccagttttcc
     8221 aagacttgat aaactcctcc ttggctgttg gctggtgcta gagcagtcgc cacagctttg
     8281 gctttctcat cagcattttc catcgcatag gcaattccag ccatttcaag catttcaaca
     8341 tcattttcac tatcaccaaa agccatgatt tcttgggatt tcaagtccca gcgcttgagt
     8401 aattcctcca agccccatgc tttatgaatc ccagcttgga ggatatcaat gcaaccatag
     8461 ccactggata cagctcggac acggccatca aagagagcat tgatttcttc caaaaccgaa
     8521 ctcaaacgtt cctcaccaac aaccatgctc atcttgagca caccaccaaa gaggtcagat
     8581 gttaattcat ccacaaattg catccgttgg tagaattttt caatcatttc tggagtcata
     8641 aaactttcaa gatctgtaaa aatcgtacct tccttgacaa aatcaccctt catccccgtt
     8701 acaacaaact ggtcctgaca cgctcgaccc ttgaaatgag tcaaagcctt gttgacaatg
     8761 gcgtcatccc atgtctgagc ctgaatcaat tcattgtttt caaaaatacg agcgccatta
     8821 gcaacaacca gaaccactcg atccaccaag ggactcagta gttgtctcat gcggtgaatt
     8881 tcattgcccg tcgcaatgac aaaacgaatg cccctttgat ccaactgatc taaaatcttt
     8941 tccaatcgtg ggagatcaag ctgacctcta gcatccagca aggtcccatc catatctgtc
     9001 gcaattacct taatcatcat ttactttcct ctggattcaa gctaatacca cttcaatccc
     9061 acatgttcat tcatttcttt ataggctgta tcaattcgtt ccttagtcgc ttcatctaac
     9121 tggtcacgat gactgccatc ctctataaaa tcttccaaaa tataagtttg gtaatgatac
     9181 aagggatatc cttctggtga aaaggttccc tttggtgttc gattgaccca agtaggatga
     9241 gctttagctg ttccgatagt tgtttttcca tccttcttct tgatggtgac atccatgaga
     9301 acaccacgtt cagtccactt agcattctct tcatctccca tagattcaat ccgttgattg
     9361 gaaatgaagt tccccattta atagacaatg agcttcttat ctccatcttt ttccaccgtt
     9421 tcagatggtt caacaacgtg aggatgccct ccaaagataa tatccgctcc ccaatcgatc
     9481 atcttgtgat aaagagcttt ttgttcttca gttggttcca atcgatactc aacacccatc
     9541 tgaagcatga taatggtgat atctgcttcc ttctctgccc gttcaatttc aaccttcatc
     9601 ttatcttcgt ttaaatctga aagataacga ttatagtctt cctgagaaat atactgctca
     9661 attccattga aaccatagga ataggctaac aatgcaacct tgataccatt cacttcctta
     9721 atgaccagcg gagcctgaac acgtggttcg tgcgtataaa ctccgattgg agtgattcca
     9781 gctttctcaa taatatcggc cgttgaaata actccctcaa gttgaaataa ctccctcaag
     9841 ttgcgaatcc aaaatatgat tatgagctaa atccagcaca tgataacctg cctccttaat
     9901 agcatccata acttcagcag gagcattaaa gagaagataa cccgctaaat aatgatcctt
     9961 attaatggtt ccttcaaaat caccaatagc taaatctgct tgcttgagcc aaggagtcac
    10021 atactcaaaa ttttcatgaa agtcataggt accgtcttct tttttagctg aaaagaaaag
    10081 tccatcgtgg tagagcaaat ccccgtgggc cataattctg gcagtttttt cctcttccta
    10141 ctcctgagac ttttgcttag tcccctcttg agaaatagta tctcgttttt gactagtcaa
    10201 gggattcctt ggaagctttc aaacaacaag accaagccct tcataaaacg aaaagctaaa
    10261 ccaccctgat ttcgatttta tcttctttgt atcttcattc tccctacttt cttatgcaag
    10321 ccttttcttt ttattatatc acagataagt atttctttca caattgaatt gaacttccca
    10381 tctattttct ataaatccta aatgccataa tgctttcaat tcttgtcatt ttgtgatatc
    10441 atgtagagga aatgaactaa tccacagtgg cttatttcaa gtataccact tgggctttgg
    10501 cagtagctaa ctgcactaaa tataatataa ggagagaaaa tgtctgcaat agaacgtatt
    10561 acaaaagctg ctcacttaat tgatatgaac gatattatcc gtgaagggaa tcctactcta
    10621 cgcgcgattg ctgaggaagt cactttcccc ctatctgacc aggaaatcat cctaggcgaa
    10681 aagatgatgc aattccttaa acattcccaa gaccctgtca tggctgaaaa aatgggactc
    10741 cgcggtggtg ttggactggc tgctccccag ttagatatct caaaacgcat tatcgctgtt
    10801 ttggtaccta atattgttga agaaggcgaa actccacagg aagcctacga tttggaagcc
    10861 attatgtaca atccaaaaat cgtctctcac tctgttcaag atgctgctct tggcgaagga
    10921 gaaggttgcc tgtctgttga ccgtaacgtg cctggctatg ttgttcgcca tgcccgcgtt
    10981 actgttgact actttgacaa agatggagaa aaacaccgta tcaaactcaa aggctacaac
    11041 tccattgttg ttcagcatga aattgaccac attaacggta tcatgtttta cgatcgcatc
    11101 aatgaaaaag acccatttgc agttaaagat ggtttactga ttcttgaata aagaaaatcc
    11161 cgttgcaaga cggggttttg tgttataata gaggcatgaa aacaaatgat attgtctatg
    11221 gcgtccacgc cgttaccgaa gccctccttg caaatacagg aaacaaactc tacctccaag
    11281 aagatctccg aggtaagaat gttgagaaag tcaaggaact agctacagaa aagaaggtgt
    11341 ccatttcttg gacatcaaaa aaatctctct ctgagattac tgaaggtgct gttcatcaag
    11401 gttttgttct acgagtgtct gaatttgcct atagcgagct agattacatc cttgcaaaaa
    11461 cacgccaaga agaaaatcca cttctattga ttctagatgg tctaaccgat ccccataatc
    11521 tgggttctat cttgcgaaca gccgatgcga ccaatgtttc aggtgtcatc attcccaagc
    11581 accgtgctgt cggagtaact cctgtcgttg ccaaaacagc cacaggtgct attgaacacg
    11641 ttccaattgc ccgagtgaca aacctcagtc aaaccttaga taaactcaag gatgaaggct
    11701 tctggacctt tggaacggat atgaacggta ctccttgcca caagtggaat acaaaaggga
    11761 aaatcgccct catcatcgga aatgaaggaa aaggcatctc tagcaacatc aaaaaacagg
    11821 tcgatgaaat gattaccatt ccgatgaatg gacatgttca aagcctcaat gccagtgttg
    11881 ctgcggccat tctcatgtac gaagtttttc taaatagact ataaaaaagt ttccagtcat
    11941 ctgattggaa acttttttat aattaactat gttctgtgat aaacttgtag gcttcttgac
    12001 cagcgatagc tccatctcca actgctgttg ttacttggcg aaggtctttc aagcgaacat
    12061 ctccaactgc aaagataccg tcgactgcag tcttcatgtg gctatctgtc acaatccagc
    12121 ctgcctgatc ttggatattc aattctttaa caaaatcgct aagagggtcc aaaccaacat
    12181 agataaagac accaccgaag gcttgctctg tcacttgacc tgttttcaca ttttcaaaaa
    12241 cgacagattc tactctgttt tcacccttga tttccttcac tacagaatcc cagataaagc
    12301 tgattttttc attcgcaaag gcgcgatctt gtaaaacctt ttgggcacga agttggtcac
    12361 gacggtgaac aatggtaaca gtcttagcaa aacgagtcaa gaagagggct tcttcaacag
    12421 ctgaatctcc accaccaact accaataaat cttggtcacg gaagaaagca ccatcacaca
    12481 cagcacagta agaaacacca cgactgttca gttcttcttc tccaggcact cccaaaggac
    12541 ggtgtttaga accagttgct acgataactg tacgtgtttc atatgtttgg tcatcagtca
    12601 tcactttctt aaaatcacca tggtcttcga cattttcaac ataaccataa atgtgctcaa
    12661 caccaagatt ttcaagtggt tcaaacatct tttcagccaa ttcaggtcca ctaatattag
    12721 cgtatcctgg gtaattttcg atatcagatg tattattcat ctgaccacct ggcagaccac
    12781 cttcaatcaa agctactttt agattgcttc gagcagcata caaggccgca gtcatccctg
    12841 caggtccagc accgataata atagtatcgt acatatagat tccttctttc ttgttgtaac
    12901 tatctttatt ctaactcttt cttgtcaatc acgcaaggat tatgccttga aaacaagaat
    12961 taaactcata accgcaaagg ataatactgg gacaaccaag actccaatca taatcatatc
    13021 atagagatgg gcttggcgag ccctggctgt cttatcaact cccgacatgg ctctcagacc
    13081 aatccaaatc cctaaaaaaa tcagaacaag gatggtggtc aagatcaaac tctcgaaata
    13141 taaagaaaat agttgcagta gcatgatttc tctcatttct atctttttta aagagtaaac
    13201 tcagctagtc caactaactg agttttcctt tatctattat atcaaatata agtccgtttg
    13261 taactagcga agaattcttt tgtccgctct tctttagggg tgtggataat ctcatccgga
    13321 gttccagact cgatgatttt ccccttatct aagaagagaa ttttatccgc aacttgggct
    13381 acaaaggaca tgtcatgact gaccaaaatc atggtctgac ctgacttagc agcatctgca
    13441 atagactttt ctacttcacc gaccaattct gggtcaaggg ctgaagttgg ttcgtctaag
    13501 agcaaaacat ctggtttcat agcaagcgca cgcgctaggg caacccgttg cttctgtcca
    13561 cctgataaat ggcgaggata atggttttca cggtccgaaa gcccaacctt agccaactct
    13621 tccttggcaa tcttagtcgc ttcttggtca gataatttct tgacaacaac caagccttct
    13681 ttcacattat caagtgctgt tcgacgttca aataaattaa actgttggaa aaccatagac
    13741 aacttacggc gtagggcaag gatttcttct tgagtgattt tagaaaaatc aactgaaaaa
    13801 ccatcaatct gaatagagcc actgtcaggt gtttcaagat aattgagact gcgaagaaag
    13861 gttgattttc cagctcctga agaaccaatc aaggctacaa cttccccttt ttgaatatcc
    13921 aagttcagat gatccaagac agtctgtcct gaaaaggatt tgcttaaatt cgaaatctta
    13981 atcattaacg aaggtctcct ttcacatctg tttgcactgt atcaggtgca gaaatagcca
    14041 tttttctctc gatgaaacga ccgaggcttt caattccgat attgactacc caataaacaa
    14101 gggcaacaga gatgaagcgt tcaaaatagc gataatcagc tccacctaga atctgagctt
    14161 gggcaaagac ttccacaaca cccgcactaa aagctagaga tgttcccttg gtcaaaccga
    14221 tgagggaatt aatcaaggtt ggagtagcta ccaccgctgc attaggaata atcactcgtc
    14281 gataaacttg cgctcgggtc atacccagac tgcgcgccgc ctcaatctca ccaggattaa
    14341 ctgagagaat ggctgcacga atggtttcac tagcataagc tgcctcatta aaggcaaaag
    14401 cgacaatcgc aaaagctgca gctggaatcg cattgatatt gagaccagtt ccccattgct
    14461 gattgagggc tttcaaagcc aaaggaattc cgtagtaggt caacatgagt tgcaccaaaa
    14521 tcggtgtccc ttttaagaaa ctaacaaaga aggcctgcaa gggatataaa atcttgacac
    14581 gattgatctt cacaatggca aaaagaagcg ccaaaaccaa gccaaaaagg gcaccaccaa
    14641 ttgtcaacat aattgttgtt ggaagttgtt ggacaattct agggattcca tcaaagaccg
    14701 aacgtaggct aaacagcttg ccatccggaa tcaattgcat caagttttgg taccaatctg
    14761 atgctaaaat cgttgtaaca ttcataaaaa catcctcttc tgataatgat tcattctacc
    14821 atgcttctgc cgtcaatgaa attatttgct acggtcagat acagactttg tctacctact
    14881 agtttatcat actttgaaac agggaagtta tatattttat ctatcaaaga gatagataaa
    14941 aactatttca gtcccagttc ttcataagat tttcgataac cgatttgctt aacagttcca
    15001 ttttctacta aaatgggccg ttttaacaac ataccgtcac ttgctagcaa ctcagccgct
    15061 tcttggtttg acaaacttcc taccttatct tttagcccta attcacggta tttgatacca
    15121 ctggtgttga aaaattgctt caattcaaat cctgaggttt ctagccaatt caaaatgact
    15181 tcttggctag gtgtttcttc cacgatatgg acggctttat agtccacacc taattgattt
    15241 aattcttgtt ttgctttttt acaagttgaa catttggggt attcgataaa ttctaacatg
    15301 tgcttctcct tctatttgtt tatatcttta aaatctacgc cttcatgctc caagagccaa
    15361 gctttctttt ccactcctgc agcatagcct gtcagacgct tgcctgctcc caacacacga
    15421 tgacaaggta ctaggataga ccaaggattg cgtcccactg ctccaccaat tgcttgagca
    15481 gaagccactt gcaggtcttg agcaatttgt ccataggtca ctgtctgacc ataaggaatg
    15541 ccctgtaaat agtcccaaac tctcttttca aaattcgttc cgattggcgc caagagcaag
    15601 ttggataaat cctgaggctt gcctttaaag taatcatcta agcaagcaat aactgggtct
    15661 aaaataggat gactaacaac ttcttctatc gtttcatctc ctagtcccct ctcaaaatgc
    15721 ttctgctcct gaacccaaat tccatacaaa taatggtcat cagctacaag tgatagaata
    15781 ccaattggtg aggagtagag gatttttaca tacttctttt gcattatttc tttaattttt
    15841 gataggccaa gcgttcgcca tctactggca ccttaccaac ttgtttaaaa ccaagttttt
    15901 caaaaatatg ttgcataacc ttgttttcag catgcgtatc tgagcgaaaa tcaagataat
    15961 caaaaccttc aatcaagccc tctaagaagg tttgagcaac tccttttccc tgcacatctg
    16021 ctgccacagc aatacggtga aagactagat actctgactc tccagcttgc cagtttccct
    16081 cataaatagc ttcataggcc tcctctggac tcttggtcac agcagcatag gctagcagtt
    16141 ctccctcttc caaggctacg taggcttgcc ctgagatgat atcatcgata ataacatctg
    16201 catttggata gccattttgc cactggttac taccagcatc tgctaaacat tttttggcat
    16261 cctctatcac ctgcatgatg gcatctactt catttggaaa agctaaacga atttccatct
    16321 tttttcctca tttctgacta gtttctccca tcatagcata atttaagtct ttctacaagc
    16381 agttaaaact tgactttgta tttttgttat tttataatct agttatcaaa actatataaa
    16441 aaggagttga aaatgaaaac taccttttcc tacccaaaat gggcagaaat tccaaacatt
    16501 aacctctatc tggaccaggt tttgctctat gtcaatcagg tctgcgcccc tatctctcct
    16561 aataaagaca aaggcctaac agcatctatg gtcaacaatt atgtgaaaaa tggttacctg
    16621 acaaagcctg acaaaaaaaa ataccaacgc caacagattg cccgtttgat tgctatcaca
    16681 accctcaagt ctgtattttc tattcaagaa atagcccaga cacttaatac tctacaaact
    16741 caagcaagtt cagaccaact ctacgatgct tttgtggact acatgaacca aggaattgac
    16801 ccagctaacc ctattatcca aaccagctgc caaaccgtta aactctatca tcaaactcta
    16861 gacttaatcg atcatactca agaggaggta atccaatgaa cactagtctt aaactcagca
    16921 aacaactcag ttttggagag gagattgcta atagcgtgac ccatgctgtg ggtgcagtca
    16981 tcatgcttat cttgctgcct atttcatcca tctatagtta tgaagcacac ggatttttat
    17041 cctctatcgg cgtttccatt ttcgtcatca gtctctttct catgttccta tcatccacca
    17101 tttatcactc tatggcctat ggttcgaccc acaaatatgt tttgcgaatc attgaccatt
    17161 ctatgattta cgttgccatt gccggctcat acacgcccgt tgtcttgacc ttgatgaata
    17221 actggtttgg ctatctgatt attgtcatcc aatggggaac gaccatcttt ggtattctct
    17281 ataaaatctt tgctaaaaag gtcaatgaga aatttagcct tgctctttac ctgattatgg
    17341 gctggttggt tctggctatc attcctgcca ttatcagtca aacgacaccc gttttctgga
    17401 gtctcatggt aactggcggg ctctgttata cagttggagc tggattttat gccaagaaaa
    17461 aaccttattt ccacatgatt tggcatctct ttatcctagc tgcgtccgca cttcaataca
    17521 tcgctattgt ttattacatg taaaaaagtt gagaaattca atctcaactt ttttctttac
    17581 acatattgat aaagtactgg tgcaagcgca catcatcagt caattctgga tgaaaagaac
    17641 ttaccaacat atttttttct tgggctgcaa caatttgatt gttcactgtt gctaaaattt
    17701 ctacaccctc accaacacta ctgataatcg gaccacggat aaaggtcatt ggaatcttgc
    17761 caactccctt acattctgct tccgtgtaga aacttcctaa ttggcgccca taagcattac
    17821 gctcgaccac catatccata gttcctagat gactctcttt ctgagaagtg atttccttag
    17881 ccagcaaaat taagcccgca caggtcccaa acactggtaa gccagataga atggcttctc
    17941 ggatgggaag tagcatgttc tggtcacgta agagcttgcc catggttgta gactcaccac
    18001 caggcaaaat caaacccgac aagtcactct gatcttgctg aaaatcatct agatttctga
    18061 gttctacact ctcgacacct aattgatcta gcacttttgc atgttctgca aaggcccctt
    18121 gcaaggccaa tattccgatt ttcatctatt ttcctcgttc agccatgaga atttggattt
    18181 cattttcatt aataccaacc atggcttctc ctaaatcttc agagatttga gctaggattt
    18241 gaggattacg gaagttagtc acagccttaa caatggcact cgctcgttta acaggatctc
    18301 ctgacttgaa aatacctgaa ccgacaaaga ccccctctgc ccctaattgc atcattaacg
    18361 cagcatctgc tggcgttgca acacctccag cagcgaaatt tacaactggc aattttccat
    18421 gttcatgaac atattggacc aattctacag ggacttgcaa atccttggca gcaacataaa
    18481 gctcgtcctc acgtaagttt tgaatgcggc gaatttcctg attcatcata cgcatatgac
    18541 gaacagcttg gactatatcc cctgtccctg gttctccttt ggtacgaatc atggaagcac
    18601 cttcagcgat acgacgcaag gcttcaccca aatccttagc accacagaca aaaggaactt
    18661 ggaattcttt cttgtccaca tggaaacggt catcagctgg agatagaact tcactctcgt
    18721 cgatataatc aatttcaata gcctctaaaa tctgagcttc aacaaaatgc ccgattctga
    18781 ccttagccat tactggaata ctaaccgctt cttggatttc cttaatcatc tttgggtcgc
    18841 tcatgcggga aactcctcca gctgcacgaa tatcagccgg aattcgttcc aaggccatca
    18901 cagctgccgc accagcagct tctgcgatac gagcctgttc aggattctgc acatccataa
    18961 taacaccacc cttgagcatc tgtgccaagt ttttatttag ttcataacga ttttcagtca
    19021 tttgttttat cctcatcatt tgtattggta gctacctttt cattttaagt cagaacagaa
    19081 tgaagcacaa gataaaaaaa ctataattgt atggggacag attagctaaa aactatctga
    19141 ccttaactta aggccagata gctcattcat ttttattttt cagccgtaag ggcagccatt
    19201 gtgatgtagt tgtatggttt gttgaagtgt ggcaagaaga agaggtctgt caatgccaat
    19261 ttatcaattg tcacatgctc ttggatagca agtgagaaca tgtggattcc catgctaatt
    19321 gcaatatcat gtgaaaccat ttgggcacca agaatttcac ggctatcttt gtcaaagaca
    19381 atcttaattg ctacttcatg gttgtcatgt ttcatgaatt ctggtttttg aagatcgtta
    19441 aagcctgttt cagttgcgtt gtaaccagca gctttcgctt tttcaagagt caaaccagtt
    19501 gaaaccatgt gaagaccgta gattgagata ccatttgaac cttgaacacc gattccttcc
    19561 aattcatgtc cacaagcatt gtaggcacca acgataccag tgcgaacagc atttgaagca
    19621 agagcgatat agcttgtatc tttacgagca ttgtcataaa cagtcgcaca gtcaccaaca
    19681 gcgtaaacgc ctgggataga tgtttcttgt ttcttgtcta caaggaaggc accgttgcgg
    19741 aagagttcga tcttaccatc tgcaagggct gtgtttggac ggaaaccaac tgcaaggata
    19801 accatatcca cgtcaaagct ttctttgtca gtaatcaagc gttcaacttt accgtcacct
    19861 tcgattgctt taacagtttg acctagagcc aagcggatgt tgtgatcttc caagttcttc
    19921 gccatcattt gtgtgaagtc tttgtcatag taaccgttca agacagtatc aacgatatca
    19981 acaaggacaa cttcttttcc aagacgttca aaggcttcag caagttcaac accgatgtaa
    20041 ccaccaccaa caacggcgat acggtcgagg tgttggctct tgtcagaaag tttattgata
    20101 acttcttcag cattttggta caatttcacg aattgtacgt tttcaagagt tgctttaaat
    20161 tcgcggtttc ctttaacaat ttcaacacct tcgattggtg gcaagattgg tgtagaacct
    20221 gtagcgaaaa tcaatttttc gtatgattct ttgtgctctt ttccttcaac ttccgctgta
    20281 actactttgt tatcatagtc gattgaaaga acaggtgagt tcatgtaaac tttagcacct
    20341 ttagcttcca atttttcttt atcagaatag aacaagcctt cagcaccgtc aatttgttca
    20401 ccaatccaaa gagccattcc acatcctagg aaagagatgt tagagttttg gtcaaataca
    20461 acaatttcgt tctcatttcc aaaattatcc aacatggtat tgatacatgc tgtaccagcg
    20521 tggttagcac cgactacaac gattttactc ataaaaattt cctgccttca ttctaaattt
    20581 accttactag tataccattt accgttagcg tttacaaggg gggtactatt atttcagcat
    20641 tttttatcaa aaataattcc ccatttatcc tgttttcaag cctcatgaat tttatgagca
    20701 tatttcttga ctttttccaa attttgtttg tgaaaattct aactttatgg tattataata
    20761 acatgaaaat gaaatgtaag ggcttcaatt tagatgatta tttgatatta caacttattt
    20821 tctataagag aaaggagttg gtagcttgac tcttcgttca cattctgaac ggctaatggg
    20881 gactactatc actatttcat tagtggatga gcaagccgat atctttctcc aaaaatcctt
    20941 tgacttgctc aaagaacttg aataccgctt caacgccaat agtcaagaat ctgagttgat
    21001 ggaaatcaat tatcaagcag gaatagcccc cgtcacggtt catccagacc tgtttgagct
    21061 gatttcactt gggttagagc atagcctagc gctctctagc cacctcaata tcagcattgg
    21121 tcccttaatt caaacctggc gtatcggttt ttcagatgcc aaggtcgccc agcctcaaga
    21181 aattgaatcg gtgctgcctc taatcaatcc tcatggtatc gagttagatt cttctacttc
    21241 tactgtgttt ttaaaacaga aaggaatgaa gatcgatcta ggttgtttag ccaaaggata
    21301 cagtgcggat aaggttgccc aatttcttag gaaagagggg gtgacttctg ccttgatcaa
    21361 tctgggaggg aatatcctga ccattggaaa aaatcaggca agaggggata acccatggca
    21421 aatcgggatt caagacccag ccaatcctag gggaaatcat ttaatgacca tccctgttgt
    21481 caataaatct gtcgtgactt caggcattta tgaacgtcac ctgaccgtcg atggacaaga
    21541 ttaccatcac atttttgaca gtcaaacagg atatcctgtt gaaacggaac tagcgagtct
    21601 aacaatcatc tctgataaat cagtcgatgg cgaaatctgg acgactcgac tctttggaga
    21661 aagacctgct tctatcctct ggcaagtcga aagtttggag ggcatcgaag tcatcctcat
    21721 cgataaagaa ggccacctaa gctgttcttc aggaattcct actctataga gacaactgtt
    21781 tcaatggaat tttaaaattg tattatgtaa aaagagaaag gaaatctcat gttaaaactt
    21841 attgctattg ttggaacaaa ttcaaaacgt tctacaaacc gtcaattgct tcaatacatg
    21901 caaaaacact tttctgacaa agctgaaatt gagcttgttg aaatcaaggc cattcctgtc
    21961 ttcaacaaac cagctgacaa gcaagtacct gctgaaatat tggaaattgc tgctaaaatc
    22021 gaagaggcag atggcgttat tatcggtact cctgagtatg atcactctat tccagctgtt
    22081 ttgatgagcg ctcttgcttg gttgtcttat ggtatttacc cacttttgaa caaaccaatc
    22141 atgattacag gtgcttctta cggtacgctt ggttcatctc gtgcccaatt gcaacttcgt
    22201 caaatcttga atgctcctga aatcaaggca aatgttcttc cagatgaatt cttgctctca
    22261 cactctcttc aagcatttaa cccaagtggc gacttggttg accttgatgt tatcaagaaa
    22321 ttggatgcca tctttgatga cttccgtatc tttgtaaaaa tcacagaaaa attacgtaat
    22381 gcacaagaat tacttcgcaa agatgctgaa gactttgact gggaaaattt gtaagatagg
    22441 agaccaaaaa gaatgaaatt tgttggactt gttggatcaa actacgatca atcatataac
    22501 cgtaagctct tggaatttat ccgtcgctac ttcaaactca aatttgaatt agaagttctt
    22561 gaaattgacg aagttccaat gtttaaccaa gatgaaaaat gggatgaaag tttccaatta
    22621 cgttatttat ataacaaaat tactcgtgca gatggtgtta tcattgctac tcctgagcat
    22681 aaccacacta tctcagcttc actcaaatct gtacttgaat ggctttcata cgaagttcat
    22741 ccatttgaaa acaagcctgt tatgattgtg ggtgcatcat actatgacca aggaacatca
    22801 cgtgctcaag ttcaccttcg taaaatcctt gatgctccag gtgttaatgc ctacacgctt
    22861 ccaggtaacg aattccttct tggtaaagct aaggaagctt ttgataacaa cggaaatatc
    22921 accaacgaag gcactgttaa attccttgaa acttgcttag ataactttgt taaatacgta
    22981 ggagtcgttt cgaaattgaa aaaaccaaaa ccaatcgaat cagaagactt ggattgtgga
    23041 aaaccaatcg ctacaaccat cacagaagtt gaccctgacg atccagaatg ggtagaaaaa
    23101 gttgcagcaa tcactggtgc tgtttctggt gatacctatg tcaaattgga ccacggtatc
    23161 ctaacagtta accaaattga tatgttcttg aaagctatgc catttgaatt gacatacgct
    23221 gacgacaaca accaattcct ctactacaac aacgctcacc aagatccaga cactatgttc
    23281 gctaaacgtg taccacctca atcaggtagc cgtatgtcga ctgttcatgg ttctcttcca
    23341 ccagcacgta tgaagaacgt agagtgggtt atcggaacac ttcgcaacgg aaaccaagaa
    23401 tacgtccgta cgatcgttcc aggttctcct gcaggtgtta tcaacactca caactaccaa
    23461 gcaatgtact atcctgatgg atcatacgct ggtatcaatg aaatcgtctt taacttccaa
    23521 ccatggcttg actggtacct aaaagagact ggtcaacgtt tggtgggtgg tagcggacca
    23581 tttgctcctg ctgccggagg tcatggagac gccgatgcta cttctggcgc ttctgattca
    23641 ggggatgctg gaggccacgg tggtgacgca gacgctacag ccggtgcaag ttactaatga
    23701 tagtttttag gaaccattat ggttcctttt ttagcgaaaa aagactagca aacacaaact
    23761 gctagtctct ttgttatcgt ttcatctatc tttgttaaaa gaaagagtac ttatactcaa
    23821 tgaaaatcaa agagcaaact aggaagctag ccgcaggctg tacttaagta cggcaaggtg
    23881 aagctgacgt ggtttgaatt tgattttcga agagtattat tatgaatttg ggtcatcaag
    23941 actacggcca tgtaatcctt tttcacgttg tacttggcgt agtttttctg gtgtaacatc
    24001 gtttccttct tcgtccacaa ttttgattcc ttcaatgtgg tgacgaacag cgcggcgata
    24061 accttcgatg tactcctcac gtagtttggc ttgttccact ttttcttctg atgttaagcc
    24121 ttctgttttt ttctttttag caagctcatt gatacgagca atttttttcg gatccatttc
    24181 ttctcctttg taataagata aagtccgttt atcaaatttt atttagtgtt aatttggtta
    24241 aaacaagcaa acttagctgc tttcttggtt agttgtgaca agattgccaa acgattttga
    24301 cggacagcct gatcttcagc cattaccata gtattttcaa agaaagcatc aatgactggg
    24361 ctaagcgcaa agagttgttt caattgctga cttgcaggtc ctgataaaac gagtgtttct
    24421 actgcttctg ccaaagcttt ttcttggtca ttctcaaata gtgctgaatc aactgtagca
    24481 accccttctg ccttctcagc caggttaaag gcacgagaaa gtgattcaac agatggttta
    24541 aaatattctt ccttgcttac ttctacgaga gcacttgctg cttccaacat atctgccaca
    24601 acaaagtttg aacctgcaag aactgcttcc ttgatatctt ttggagtaga gcccatcatc
    24661 ttatcaacac gagccttgat aaagtccata acctctgctt tattttcata agtcaaactg
    24721 tcaaatttca atgcataaag gctatcaatc agctcatcca tagcaatgtg ccaaccaaag
    24781 gcatccaaga tacgaaccac accttgagtt gcacgacgaa gggcataagg gtcattagaa
    24841 cctgatggaa tcaatcctac tgagaagaaa ctcaaaatcg tatccaattt gtctgcaatg
    24901 gctagaactg cgccgacctt gctctctgga agttctcctt cagctgatgt aggcatgtag
    24961 tgttcacgaa tagcagctgc caccgctgga gtttcaccag caagaagggt gtatttttca
    25021 cccataattc cttggagttc gtcaaattca ccaaccatac ctgtcaataa gtcaaacttg
    25081 taaatggctg ctgcacgagc aaggtcaact gtttcatcca ctgacaaacc agctttttct
    25141 gccaaaagta cagtgatttg acccgtacga atcatgtgtt cacgaaggga accaatcttc
    25201 tcatggaagg tgacattgtt taatttttca acaagatctg aaatcaccaa tttttggtct
    25261 tcacgccaga agaattctcc gtcttccaag cgggctacca agactttttc atttccttta
    25321 atgacatttt tcaaacgctc tgcgtttccg ttacgaacag aaatgaagtt tggcaagagt
    25381 tttccatctt gatcacgaac aacaaagtaa cgctggtgtt ccttcataga agtcaccaag
    25441 acttcttctg gaacttcaag gtatttagca tcaaaacttc ccatgaaggc agttgggtat
    25501 tcaaccaaat tcaagacttc attcagcaaa tccgcatcaa tttcgatacg tacaccatgt
    25561 tttgcctcaa tttccttgat ttggtcaaca atcatttgct cacgttcaca tggatctgcg
    25621 attacaaact gcttacgaag gtcttcttca tagctcaatg ctgactgaat tttggtttct
    25681 ttccccaaga aacgatggcc acggctcaca cgacttccct tgatatcaag gaaatccaag
    25741 tcaaactctt gctcatccaa gagaacagtt aaagtgtgga caggacgaat gtattcaaag
    25801 ctatttccag cccagtgcat gctgacaggg aaagtcaatg acttcaagac atctacaaca
    25861 ccaggaacaa tggcttcaac tgcttgacca acttcttcct tagtgacata gacatattct
    25921 tcacccttga tttcacggaa ttcgatatct tcaacagtca agccttttcc acggacaaat
    25981 ccttgagctg ctttggtaaa gtttccatca ctatccaagg caattttctt tgctggtccc
    26041 ttgaaatctt ctgtcaaatc agactgtttg tctgataaac cagtcacacg aacagccaaa
    26101 cgacgtggtg ttgagaaggt ttgaatggct tcaaaagaca ggcggttttc cttgaggaag
    26161 gctgccattt tttcgcctag ttgtttttca cttggtgtga caacataggc tggtaattct
    26221 tcaagaccga gttctactaa taagtttttt gtcatgtttt ttcctttaca ttttcttcaa
    26281 tcttttttga tatgcacctt aggtactggg gacgtcgcta actaactctg tgagtctgta
    26341 tggaaatcca atgctaaatt tatcaagatt tccaacaatc tcatcaaagt ggatttagct
    26401 gactgatttt tgaacctaag gtttcaaaaa tccccacata acagtacggc ggtgcatatc
    26461 cctctagcaa gtcttcgact tgcctctaac gattttagag tacagtatta ctcactattc
    26521 tgcgtcttct gctaaaagtt taactcgtgt ttcttcatcc aaaagtgggt aacctaggcg
    26581 tttgcgttct gcaacaaagg ttttggctac aacacgggcc aagttacgga tacgagcgat
    26641 atagcctgca cgctctgtta cagatacggc accacgcgcg tcaagcagat taaaggtatg
    26701 tgaacatttg agaacatagt cataggcagg gtgtaccaag ccttcttcta atgcacgacc
    26761 agcttctttt tcaaacttat caaagttttc aagcaaaatt tcttggtccg aaatttcaaa
    26821 tgaatatttt gagtgctcat actcaggctg gataaagatt tctccgtatt ttacaccatc
    26881 agcccactcg atatcataga cagagtctac ttcttgaatg taagaagcca agcgctccaa
    26941 accataggta acttccgcag tcacagggcc agttgccaat ccaccgactt gttggaaata
    27001 agtgaactga gtgatttcca ttccgtcaag ccaaacttcc caaccaagac cagctgaacc
    27061 agttgatggg ttttcccagt tgtcctcaac aaaacgaata tcgtgctcca aaggattgat
    27121 tcccaatttt tccaaagact caaggtaaag ttcttggata tttgatggag aaggcttcat
    27181 gaccacctgg aattggtggt gttggtagag acggttaggg ttttccccat aacgaccgtc
    27241 agcaggacga cgtgatggct ctacataagc tgcattccat ggctcaggtc cgatagcacg
    27301 aaggaaagtg taaggactca ttgtccccgc acctttttca ttgtcataag cctgcataag
    27361 catacaacct tggtcattcc aaaattgttg caaagtcaaa ataatttctt gaaatgttaa
    27421 tttcttagac attgtttact ccttaataaa aaatgatttc ttgcgacacg agtct
//
LOCUS       DAVBNO010000024        26827 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVBNO010000024 DAVBNO010000000
VERSION     DAVBNO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.024"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            393..1571
                     /locus_tag="VQ846_001134"
     CDS             393..1571
                     /locus_tag="VQ846_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET3075714.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     LVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            1885..2016
                     /locus_tag="VQ846_001135"
     CDS             1885..2016
                     /locus_tag="VQ846_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET3075715.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2037..3587
                     /gene="dltA"
                     /locus_tag="VQ846_001136"
     CDS             2037..3587
                     /gene="dltA"
                     /locus_tag="VQ846_001136"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET3075716.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEDLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3584..4828
                     /gene="dltB"
                     /locus_tag="VQ846_001137"
     CDS             3584..4828
                     /gene="dltB"
                     /locus_tag="VQ846_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET3075717.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            4842..5081
                     /gene="dltC"
                     /locus_tag="VQ846_001138"
     CDS             4842..5081
                     /gene="dltC"
                     /locus_tag="VQ846_001138"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET3075718.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5074..6342
                     /gene="dltD"
                     /locus_tag="VQ846_001139"
     CDS             5074..6342
                     /gene="dltD"
                     /locus_tag="VQ846_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET3075719.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6497..6937
                     /gene="adcR"
                     /locus_tag="VQ846_001140"
     CDS             6497..6937
                     /gene="adcR"
                     /locus_tag="VQ846_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET3075720.1"
                     /translation="MRQLAKDIDAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            6937..7641
                     /locus_tag="VQ846_001141"
     CDS             6937..7641
                     /locus_tag="VQ846_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET3075721.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7634..8440
                     /locus_tag="VQ846_001142"
     CDS             7634..8440
                     /locus_tag="VQ846_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET3075722.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8450..9955
                     /gene="adcA"
                     /locus_tag="VQ846_001143"
     CDS             8450..9955
                     /gene="adcA"
                     /locus_tag="VQ846_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET3075723.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGKEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(11117..11890)
                     /locus_tag="VQ846_001144"
     CDS             complement(11117..11890)
                     /locus_tag="VQ846_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET3075724.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            12066..13469
                     /locus_tag="VQ846_001145"
     CDS             12066..13469
                     /locus_tag="VQ846_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET3075725.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPLLTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            13584..14222
                     /locus_tag="VQ846_001146"
     CDS             13584..14222
                     /locus_tag="VQ846_001146"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET3075726.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            14234..14674
                     /locus_tag="VQ846_001147"
     CDS             14234..14674
                     /locus_tag="VQ846_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HET3075727.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            14667..15098
                     /locus_tag="VQ846_001148"
     CDS             14667..15098
                     /locus_tag="VQ846_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET3075728.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            15089..15559
                     /locus_tag="VQ846_001149"
     CDS             15089..15559
                     /locus_tag="VQ846_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET3075729.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            15584..16357
                     /locus_tag="VQ846_001150"
     CDS             15584..16357
                     /locus_tag="VQ846_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET3075730.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            16354..17154
                     /locus_tag="VQ846_001151"
     CDS             16354..17154
                     /locus_tag="VQ846_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET3075731.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            17213..19507
                     /locus_tag="VQ846_001152"
     CDS             17213..19507
                     /locus_tag="VQ846_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET3075732.1"
                     /translation="MKLWYKKAAANWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKGCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            19517..22633
                     /locus_tag="VQ846_001153"
     CDS             19517..22633
                     /locus_tag="VQ846_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET3075733.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            22690..24456
                     /locus_tag="VQ846_001154"
     CDS             22690..24456
                     /locus_tag="VQ846_001154"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET3075734.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHTQKGIPAFGIYGRDVQEANDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVERVTGYTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELDESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            24602..25753
                     /locus_tag="VQ846_001155"
     CDS             24602..25753
                     /locus_tag="VQ846_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET3075735.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            25898..26782
                     /locus_tag="VQ846_001156"
     CDS             25898..26782
                     /locus_tag="VQ846_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET3075736.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
BASE COUNT         8384 a         4418 c         5705 g         8320 t
ORIGIN      
        1 actcccctga aatttatttt aattatgcaa atttcacgta tttttgatgc tgagacgacg
       61 atcctgggaa cttttcagat atttttttga ctatctaaat ctatcattag aaaagcttag
      121 agcgccaaag gatttgagcg tttttctgat ttttaagact ttttccagtc tctttttcga
      181 ttgaagatgt aattattcta ctaactaact aactaacttc ttagtactag ccaacaacga
      241 taatcataat tcctcctaaa attaggaata ataaaggcaa tagtttttgt tttttcatgt
      301 aaaaaacctc acttttgttt tctgctattt tatgctaaaa tattaaaaat caaatttaat
      361 tccaaagttt gtaactaaag ggggagcgct acatgtctaa ttcatttgtc aagttgttag
      421 tctctcaatt atttgcaaat ttagcagata ttttctttag agtaacaatc attgctaaca
      481 tatacattat ttcaaaatca gtaattgcca catcactagt tcctatctta ataggaatat
      541 cctcttttgt tgcgagtctt ttagttccgt tggttactaa aaggttagcg ctaaataggg
      601 ttttatcttt atctcaattt ggaaagacta tattattggc gatactggta ggaatgttta
      661 ccgtaatgca atccgtagcg cctttggtga cctatctatt ggttgttgca atttccatac
      721 tagatggttt tgcagcaccc gtttcctatg ctattgtgcc acgctatgcg accgatttgg
      781 gtaaggctaa ttcagcctta tcaatgactg gtgaagctgt tcaattgata ggttggggat
      841 taggtggact cttgtttgca acaattggtc tgttacctac cacgtttatc attttagtct
      901 tgtatatcat ttctagcttt ctgatgttat ttcttcctaa cgctgaagtg gaggtgttag
      961 agtcagaaac taatcttgaa attttgctca aaggttggaa gttagttgct agaaatccta
     1021 gattaagact ttttgtatca gcaaatttat tggaaatttt ttcaaatacg atttgggttt
     1081 cttccattat acttgttttt gtaacggagt tattaaataa aacggaaagt tactggggat
     1141 attctaatac agcatactct attggtatta taattagtgg cttaattgct tttaggctat
     1201 ctgaaaagtt ccttgctgct aaatgggaaa gtattctttt tcctcttgta gctatggcaa
     1261 tagtgacact gactattcta tattttccaa acgcacagat gtttttatta ttttcagctt
     1321 tagttggtat gttatcgcaa ctaaaagaag ttcctgaaag tgtatttctt caggaaacag
     1381 tagaggaaaa tcatttagtt aatgtctatt ccgttcttga agtgatttct acattagcat
     1441 tttcagtatt tgttttgcta atgagctata ttactgagag ttttggtatt agcatcagtt
     1501 tttggctatc agccatttgt ctgatgatag aagctatttt aatttatatc agacgagatt
     1561 attttaaatg agagagtggt taaaaagtct ttaaaaatca gaaaagcgca tagtatcagg
     1621 tgttgaaaaa acttgatatt gtgtgtttta ttatgggaag atttacttca ttgtctcctg
     1681 aaatggagtt ttgaggcagg atcactctat ttgaaaatat acatagaaaa actgtataat
     1741 ccttaaaata agctgaaatc tattggaaac atacggtaaa caattgaaaa gatactatat
     1801 aaagtggtac aatggtagaa aaataagcta gtaagaatca ctcttatttt agcgaaaaat
     1861 tacagaaatg aatggttttt cttgatgaaa cagagaaaag aattgtacct ctttcttggt
     1921 cggacagcct tgtattttct tatctttcta gggctgcttt acttctttag ctatcttggt
     1981 cagggtcaag gaagctttat ctataatgaa ttttaacttg aaggagaatc cctattgtgt
     2041 caaataaacc aatagcagat atgattgaaa ccattgagca ttttgctcag acacagccta
     2101 gctatcctgt ctataatgtt ttggggcagg aacacactta tggcgattta aaggctgatt
     2161 cggatagttt ggctgcagtc attgaccaac taggcttgcc tgagaagtct cctgtggttg
     2221 tttttggtgg ccaagaatat gaaatgttgg caacctttgt agcgctgact aagtcaggtc
     2281 atgcctacat tccaattgat agccattcgg ccttggagcg agtttcagct attttagaag
     2341 tagcagagcc aagcttgatt attgccattt cagcctttcc cttggagcag gtttctacac
     2401 caatgataaa tctagctcag gttcaagaag cctttgccca agggaataac tatgaaatca
     2461 cgcatccagt caagggagat gataattact acattatctt tacttctggt acgactggta
     2521 agcctaaggg agtgcagatt tcacatgata atctcctcag ctttacaaac tggatgatta
     2581 cggataagga atttgcgaca ccgagtcgtc cgcaaatgct ggcacagcca ccttattctt
     2641 ttgacttgtc tgtcatgtat tgggcaccga ccttggcact tggtggtacg cttttcactc
     2701 ttccttcagt catcactcag gactttaagc aactctttgc ggctatcttt tcattgccaa
     2761 tcgctatctg gacatcaaca ccatcctttg cagatatggc catgttgtct gaatacttca
     2821 acagtgagaa aatgcctgga atcacgcatt tctactttga tggtgaagaa ttgacggtca
     2881 aaacagctca aaaactgcgc gagcgtttcc caaatgcccg tatcatcaat gcttacggcc
     2941 caacagaagc gacagtagct ctgtcagcag ttgccgtgac agacgagatg ttagcgactc
     3001 tcaaacgcct accaatcggc tataccaagg ctgattctcc aacctttatc attgacgagg
     3061 aaggaaataa actgccaaat ggtgagcagg gagaaatcat tgtttctggg ccagctgttt
     3121 caaaaggtta tatgaacaat cctgaaaaaa cagcagaagc cttctttgag tttgaagatc
     3181 tgccagccta tcacacaggc gatgtgggaa ccatgacaga tgagggcttg cttctctacg
     3241 gcggacgcat ggacttccag attaagttta acggttaccg cattgagtta gaagatgtct
     3301 ctcaaaacct caacaagtct cgctttatcg aatctgctgt cgcagtaccg cgctataaca
     3361 aggaccacaa ggtacaaaat ctattggctt atgtcatctt aaaagacggt gttcgtgagc
     3421 agtttgagcg agatatcgat attaccaagg ccatcaagga agacctgaca gacatcatga
     3481 tgtcctatat gatgccatct aaattccttt accgagacag tttgccacta actccaaatg
     3541 gaaagattga catcaaagga ttgattaacg aggtgaataa gagatgatgg agttttttca
     3601 acagcttcct catttagagc catatggcaa tcctcagtat tttgtttatg tgattgctgc
     3661 aaccttgccc atctttatag gtctcttttt caagaaacgc tttgcctggt atgaagtgtt
     3721 ggtaagtctc ttctttattg tcaccatgtt ggtgggtgga aagaccaatc aactagctgc
     3781 cttgggtatt tacctttgct gggaaatatt gctcctgctt ttctacaagc attatcgaaa
     3841 aagcaaggat ggcaagtggg tcttctactt agttagtttt ctgtccctac ttccgattat
     3901 ctttgtcaag gtgcaaccag ctatcaatgg aacgcagtct ttgcttgggt tcttgggaat
     3961 ttcttacctt acctttcgtt cggttgggat tgtcatcgag ctgagagatg gagtgattaa
     4021 ggattttacc ctctgggaat tcctccgttt ccttctcttc atgccaactt tctcgagtgg
     4081 tccaatcgat cgctttaagc gatttaatga aaattatcag gctattcctg agcgagatga
     4141 gttgatggat atgctggatg aatctgtccg ctatatcatg tggggctttt tgtataagtt
     4201 tatcctagct catgttttag gagagacctt actacctcct ctgaagaatt tagccttgca
     4261 gtcaggtggc ttctttaatc tctatgcctt ggcagttatg tatacttttg gtctggaact
     4321 cttctttgac tttgcaggtt attctatgtt tgctttggcc atctcaaact tgatgggaat
     4381 ccgtagccct atcaacttta acaagccctt tttatcaagg gatttaaagg agttttggaa
     4441 tcgctggcat atgagtctgt ccttctggtt ccgtgacttt gtctttatgc gaatggtgat
     4501 ggtgttaacc agaaagaaag tctttaaaaa tcgtaatgta acctcaagca tggcctacat
     4561 tgtaaatatg ctgattatgg gattttggca tggtgtgacc tggtactata tcgcctatgg
     4621 actctttcat ggactaggct tggtcatcaa tgatgcctgg gttcgcaaga aaaaaacgct
     4681 caataaggaa cggaaaaaag cagggaaggc tgccctacct gagaatcgct ggattcagtt
     4741 gcttggcatg gttgtcactt tccatgttgt catgttgtca ttcttaatct tttctggatt
     4801 cttgaataat ctatggttta aaaaataaaa ggaaataaaa tatggatatc aaatcagaag
     4861 ttattgaaat tattgatgag ttgtttatgg aagatgtttc tgacatgatg gatgaagatc
     4921 tttttgatgc aggtgtcttg gatagtatgg gaacggttga gttgattgtg gagattgaga
     4981 accgttttga cattcgtgtc cctgtaacag agtttggtcg tgacgactgg aatacagcta
     5041 ataaaatcat agctggtatt gtggagctac aaaatgctta aacgcttatg gatgatcttc
     5101 ggaccggtct tgatcgctgg tttgttggtt tttctgctca ttttctttta tcctactgag
     5161 atgcatcata atctaggagc tgaaaagcgt tcagcagtgg ctactactat cgatagtttt
     5221 aaggagcgaa gtcaaaaagt cagagcacta tctgatccaa atgtgcgttt tgttcccttc
     5281 tttggctcta gtgaatggct tcgttttgac ggtgctcatc ctgcggtatt agctgagaaa
     5341 tacaatcgtt cctaccgtcc ttatctttta ggacaggggg gagctgcatc gcttaaccaa
     5401 tattttggaa tgcaacagat gttaccacag ctggagaata aacaagttgt gtatgttatc
     5461 tcacctcagt ggttcagtaa aaatggctat gatccagcag ccttccagca gtattttaat
     5521 ggagaccagt tgactagttt tctgaaacat caatctgggg atcaggctag tcaatatgca
     5581 gcgactcgct tactgcaaca gttcccaaac gtagctatga aggacctggt tcagaagttg
     5641 gcaagtaaag aagaattgtc gacagcagac aatgaaatga ttgaattatt ggctcgtttt
     5701 aatgaacgcc aagcttcctt ttttggtcag ttttcggtta gaggctatgt taactacgat
     5761 aagcatgtag ctaagtattt aaaaatcttg ccagaccagt tttcttatca ggcaatagaa
     5821 gatgttgtca aagcagatgc tgaaaaaaat acttccaata atgagatggg aatggaaaat
     5881 tatttctata atgagcagat caagaaggat ttgaagaaat taaaggattc tcagaaaagc
     5941 tttacctatc tcaagtcgcc agagtataat gacttgcagt tggttttaac acagttttct
     6001 aaatctaagg taaacccgat ttttatcatt ccacctgtta ataaaaaatg gatggactat
     6061 gctggtctac gagaggatat gtaccaacaa acggtgcaga agattcgcta ccagttggaa
     6121 agtcaaggtt ttaccaatat agcagatttt tctaaggacg gcggggagcc tttctttatg
     6181 aaggacacca ttcaccttgg ttggttgggt tggttggctt ttgacaaggc agttgatcct
     6241 ttcctatcca atcccacacc agctccgact taccatctga atgagcgctt tttcagcaaa
     6301 gattgggcga cttatgatgg agatgtcaaa gaatttcaat agataatata atatagaaga
     6361 gttcaagaat gaaactagtt ttcacgtttt ttcttgactc tttttttgct tgtgatataa
     6421 ttaactggta aacaaaatgt caaagaatag tatttttctc ttaaaaaaga gaagtctaaa
     6481 aggaaaggag tcagatatga gacagctagc aaaggatatc gatgcttttt tgaatgaggt
     6541 gattttgcag gcggaaaatc agcatgaaat cctaataggt cattgcacta gcgaggtggc
     6601 cctgaccaat actcaggagc atatccttat gctcttgtca gaggaatctt taacaaattc
     6661 agaattggcc cgtcgtctca atgtcagtca ggcggcagtt accaaggcca ttaagtcttt
     6721 ggtcaaggaa gggatgttgg aaacatctaa agattctaaa gatgcgcgtg tgatttttta
     6781 tcagttgact gacttggctc gtccaatcgc tgaggagcac caccatcacc atgagcatac
     6841 acttttaacc tatgaacaag tggcgactca gtttactcca aatgaacaaa aagtgattca
     6901 gcggtttttg actgctttag taggagaaat caaataatga gatatattac ggtagaggat
     6961 ttgtccttct attatgataa ggagcctgtt cttgaacata tcaattattg tgttgatagt
     7021 ggggaatttg ttaccttgac tggggaaaat ggagcggcta agacgacgct catcaaggct
     7081 agtcttggaa ttctgcaacc acgcattgga aaggtggcta tttcaaagac aaatacgcaa
     7141 ggtaagaaat tgagaatagc ctatcttcct caacaaattg ccagttttaa tgctggtttt
     7201 ccaagtacgg tctatgaatt tgtcaagtcg ggtcgctatc cgagaaaagg ctggttccgt
     7261 cgtttgaatg ctcatgatga ggagcatatc aaggctagtc tggactcagt tggcatgtgg
     7321 gaacatcgag acaaacgctt ggggtctcta tctgggggac aaaagcagcg agcggtgatt
     7381 gcgcgtatgt ttgcttctga ccctgatgtg tttatcctag acgagccgac aacggggatg
     7441 gatgcaggaa gtaaaaatga attttacgaa ctcatgcacc acagcgccca tcatcatggc
     7501 aaggctgttt tgatgattac ccatgaccct gaagaagtta aggattatgc ggatcgcaat
     7561 attcatctag tccgtaacca agactcgcca tggcgttgtt tcaatgttca tgagaatggc
     7621 caggaggtgg gccatgctta gtttattatc ttacgacttt atacaacgcg cctttttggc
     7681 ggttattgct atgagtcttt tctcaccggt attgggaacc ttcctcatct tgcgtcgtca
     7741 gagtttgatg agtgataccc ttagccacgt ctcactttca ggtgtagcct ttggtctggt
     7801 tttggggatt tctccaactg tttctactat tgccattgtc ttgattgcgg cggtctttct
     7861 ggagtatctc cgtacggttt acaagagctt tatggaaatc gggacagcta tcctcatgtc
     7921 aacaggtctg gctgtttctc tgattgtcat gagcaagggt aaaagctcga gttcaatgag
     7981 tttggaccaa tatctctttg gttcgatcgt gactatcagt gaagaacagg tcatttccct
     8041 ctttgtcatt gcggcggttg ttttgatttt gacctttctc tttcttcgtc ctatgtatat
     8101 cttaactttt gacgaagata cggcctttgt ggatggcttg ccagttcgta ccatgtccat
     8161 tctttttaac atggtgacag gggtggctat tgcccttatg attcctgcag caggagctct
     8221 tctggtatcg accattatgg tcttgccagc tagtattgcc ctgcgtctgg ggaaaaactt
     8281 taaatcggtt atgctgcttg ccagtgcgat tggctttttg ggaatggtag caggacttta
     8341 catttcctac tatgcagaaa cacctgcaag tgcaagtatt accattattt ttgtaactgt
     8401 ctttatacta atcagtttag taagacgttt tatcaaatag gagacgaacg tgaaaaaaat
     8461 tagcttatta ctagccagtc tatgtgcctt gtttttagtg gcttgttcca atcaaaaaca
     8521 ggcagatggt aaactcaata tcgtgacaac cttttaccct gtctatgaat ttaccaagca
     8581 agtcgcagga gatacggcta atgtagaact cctaatcggt gctgggacag aacctcatga
     8641 atacgaacca tctgccaagg cagttgccaa aatccaagat gcagatacct tcgtttatga
     8701 aaatgaaaac atggaaacat gggtacctaa attgctagat accttggata agaaaaaagt
     8761 gaaaaccatc aaggcgacag gcgatatgtt gctcttgcca ggtggcaagg aagaagaggg
     8821 agaccatgac catggagaag aaggtcatca ccatgagttt gacccccatg tttggttatc
     8881 accagttcgt gccattaaac tagtagagca catccgcgac agcttgtcag cagattatcc
     8941 tgataaaaaa gagacctttg agaagaatgc agctgcctat atcgaaaaat tgcaagcctt
     9001 ggataaggct tacgcagaag gcttgtctca agcaaaacaa aagagctttg tgactcaaca
     9061 cgcagccttt aactatcttg ccttggacta tggactcaaa caagtcgcaa tctcaggact
     9121 ttctccagat gcagagccat cagctgctcg cttggcagaa ttgacagagt atgtcaagaa
     9181 aaataaaatc gcctatatct attttgaaga aaatgcctca caagcccttg ctaacacact
     9241 ttcaaaagaa gcaggtgtca aaactgatgt cctcaatcct ttagaaagtc tgacagaaga
     9301 ggacaccaag gctggagaaa actacatttc cgtgatggag aaaaacctca aggctttgaa
     9361 acaaacaaca gaccaagaag gcccagcaat cgaacctgaa aaggcagagg ataccaagac
     9421 agtccaaaat ggttacttcg aggatgcagc tgtcaaggac cgcaccttga gtgactatgc
     9481 aggtaactgg caatcagttt atcctttcct tgaagacggc acgtttgacc aagtctttga
     9541 ctacaaggct aagttgactg gtaagatgac ccaggctgag tacaaggctt actatacaaa
     9601 aggctatcag acagatgtga ctaagattaa cattactgat aatactatgg aatttgttca
     9661 aggtggacaa agcaagaaat acacttacaa gtatgtcggt aagaaaattt tgacttacaa
     9721 gaaaggcaat cgtggcgtgc gtttcctctt tgaagccaca gatgctgacg ctggacaatt
     9781 caagtatgtt cagtttagtg accacaatgt tgccccagtt aaggcagaac atttccatat
     9841 cttctttgga ggcacaagcc aagaagccct ctttgaagaa atggacaact ggccaaccta
     9901 ctacccagat aacctatctg gccaagaaat cgcccaagaa atgttggcgc attgatgaga
     9961 aatcgactag ttcataagag ctagtcggtt ttttgatact agatggctaa cttcattata
    10021 gacctttcag aactttaaac aataaataaa actcttgaaa tatctgggct tatatgctag
    10081 aatgtagtta gtgtataagg ttcattagaa caccaaagcc cctaactagt gccatagtta
    10141 agggcttttt tgacgtatct tagcgattta acgggtgttt ataggctagt cagtcggtct
    10201 acttcttacc atctagccat ttgcagacga aatacaagat aattccagct ataacatctg
    10261 ctataatagt aagagcaagc tctggtataa ggctcattag tttcacctcc tctcacgaac
    10321 ccataggaac gtaatcggta accgatgaca aaagtattat atcacactaa atttagatca
    10381 tcaacctcac ttaatgcttg aactattgta gattatatgc taaaatgtag ttaattgata
    10441 aggttcatta gaacaccaaa gcccctaact atggccgtag ttaagggctt ttttgacgta
    10501 tcttagcgat ttaacgggtg tttataggct agtcactcgg tctacttctt actatctagc
    10561 catctgcaga cgaaatacaa gataattcca gctataacat ccgctataat agtaagagcg
    10621 agctctatga taaggctcat tagtttcacc tcctctcacg aacccatagg aacgtaatcg
    10681 gtaaccgatg acaaaaatag tataccacaa tacatttaga tcatcaaggt cacttaattc
    10741 ttgaaatata gggtctaggc gacaaagtct ttaaaatcag aaaaacgcat aatatcaggt
    10801 gtgcaaaaac ttgatactat gcgttttatt gtgggaaggt ttactccatt ttctcctgaa
    10861 attgagtttt tgtccagcct ctgtttttag ggttgctgag aaaataatgt catgtggtga
    10921 atatttgtaa atcagtcagc agacagaacg aatactcttc gaaaatctct tcacatcatg
    10981 tcagcttcgt ctttccgtat atatgtgact gacttcatca gttctatcta caacctcaaa
    11041 acagtgtttc gagctgactt catcagtttt caaatctgta ctttgagcaa gctgagacta
    11101 gcttcctatt tgattttcat tgaatatcag aaacccattc tccatcaaat aattcgactg
    11161 cgtctaataa tttttgatct ggcacggtgt ctgaaataaa ggttgtgtat ttggagaggg
    11221 gattaatttt aaaaaatcca gtcttgtaaa atttagaact atcaatcagt aagatggttt
    11281 catgggcttt gtcaataata ttcttttttg aaatagcttg gctgagagaa gcttcataaa
    11341 catattggtc atcaatacct cttgctgaac aaaatgctaa atcgatatta aaatgatcta
    11401 ataaagaatt ttccttatca tagttgacca cggaacagga ttgatgtttg acctcgccag
    11461 atgtgataaa gattttggag ctatctttaa cagtttcaga tagggtttgt gcagtatgta
    11521 aaccatttgt aaaaataatc aaattatcaa gttcagaaag atagggacaa agttcgtaga
    11581 cagtagtact ggaatccaga taaacacaca taccagaccg aataaagtct ttagcgagac
    11641 tagcgattag tcttttttgc ctagtacttt ctccttcccg tatttgatga gaaagttcaa
    11701 ttgtgttcat agaggacagg gtcacgtatc cgtgctttct tttgataaga ccttgatttt
    11761 ctaagaaaat taaatcacga cgtaaggtac ttgtgctgga gaaagtgatt tctgccagct
    11821 cttttacggc aattcttttt ttctttttaa taatttcaat caattcaagt acacgttcat
    11881 cttttatcat aagctcctcc taatttatca tttcaactat attatagcac aaattggagg
    11941 aatttgaatt atttttatga atattgggtt aacatttgaa cattattcaa gtaagcgttc
    12001 acatattgaa aaaataaaac gtggggatta taataaagtt aatcaaggac gaagagagaa
    12061 gaaaaatgga agcggtttta gcaatagatt taggtgcgac ttctggaaga gcaatcgttg
    12121 gttacctttc tgaaaataaa ctagtaatgg aagaaataaa tcgcttttct aatctaccta
    12181 ttagagtaaa agggcattta tcttgggata ttgactttct actagctaaa attcttgaaa
    12241 gtatccgctt ggctaatact agttacaaga ttttatctat cggtattgac acatggggag
    12301 ttgattttgg actgattgat aatgaaggta agctgttatt acaacctgtt cattatcgtg
    12361 atgaaagaac aaagggagtg ttaaaggaaa tatctgaaat gactgaatta gaaaaactgt
    12421 attcagagac aggaaatcag attatggaga taaatacctt gtttcaactc tttaaggcac
    12481 gtcaagaatc tcctgactct ttctataaga ccaataagat tcttttaatg ccagatttgt
    12541 ttaattatct cttgacaggt aagtttgcta cagaaaaaag cattgcttca acaactcaat
    12601 tatttgatcc taggagtcaa aactggaatc agaatatctt aaaactattt gaattggatt
    12661 catctttact tcctgagatt gtttcagagg gaaatgttct tggaaggata aaaaaggagt
    12721 atggtttagg cgatattcct gttgtgaatg tttgtagtca tgatacagca agcgcgattg
    12781 tctcagtacc taagacagaa ggtagtttat ttatttcatc aggtacttgg tctttggttg
    12841 gagtggaact tacttcaccg cttcttacta ccgaatcctt cagttatgga tttacaaatg
    12901 aagtcggtaa agatggagtg attacatttc tgaagaattg tacagggttg tggatcatag
    12961 aggaactaag acgttcattt gaacgaagag ggaaagccta ttcttttgat gatattagga
    13021 caatggtgga gaaagaaaaa gaaaatcttc ctctgattga tactgaatca actgaatttg
    13081 caacagaatc tgatatgcac aagactttga cagaatatct agcttatcat catgaaacta
    13141 gagagtggac agatggacaa ctatttaaga ttgtttatga aagcctagct gaaacgtata
    13201 ggaaagcgat agagttacta gaagaactaa ctcataaggt ttataagagg atatatgtga
    13261 ttggaggagg tgctagagcc agttacttta accaaatgat tgctgataga actggtaaag
    13321 aggttcttac aggtttgact gaggctacag ctgtggggaa tattgttgtg cagctcatag
    13381 ctatgggaca attaaaaggg atggaagagg ctcaccatgt tattgaggag tttctacaat
    13441 tagagagtta ttactcccaa aagaattaaa aagattgaga gtttgtaaat ttgcctccct
    13501 cccccttctt agcttttgtg caggaagggg ggataattgg tgaattgaaa aatatttagt
    13561 gttttgatat gaggaggaca aggatgtcag atgtaaaaca agaattaatt aaatatggta
    13621 agaagctagt agaaacagat ttgacgaaag gaacaggtgg gaatctcagc gttttcgatc
    13681 gtgaaaaaca attgatggca attaccccgt cgggtattga tttctttgaa atcaaagaat
    13741 ccgatattgt agtgatggat attaatggaa atgttgtaga gggagaacgc ttgccatcta
    13801 gcgaatggta tatgcatttg attcaatatc aaactcgtga tgatatcgat gcaattatcc
    13861 atgctcatac aacttatgca acagtattag cttgtctcag agaaccactt ccagcgagtc
    13921 attatatgat tgcagtggca gggaaagatg ttcgggtagc tgagtatgca acatatggca
    13981 cgaaagaatt ggctgtgaat gcagctaaag caatggaagg tcgtagagca gttttactag
    14041 cgaatcatgg aattttagca ggtgcacaaa atttattgaa tgcatttaat attgttgaag
    14101 aagttgaata ttgtgcaaaa atttattgtt tagctaagaa ttttggagag ccagtagttc
    14161 ttcctgatga ggagatggaa ttgatggcag aaaaatttaa aacatacggt cagagaaaat
    14221 agggaggata ttaatgttaa aacatatacc gaaaaatatt tctccagatt tattgaagac
    14281 tttaatggaa atgggacatg gagatgaaat agtattagct gacgcgaatt atccttctgc
    14341 ctcatgtgca aataagctaa ttcgttgtga tggtgtaaat attccagaat tattagattc
    14401 cattctgtat ttaatgccat tagatagtta cgtcgatagt tcaattcagt ttatgaacgt
    14461 tgtttcgggt gatgatattc ctaagatatg gggtacctat agacagatga ttgaaggtca
    14521 tggtacagat cttaaaacga ttacttatct tagaagagaa gacttttatg aacgtagtaa
    14581 gaaagcttat gctattgttg ctacaggaga aacttcactt tatgctaata ttatccttaa
    14641 gaaaggagta gttgttgaaa gagaaaatgt ttaatagagg aattttagtt gccagtcatg
    14701 gtaattttgc tagcggagct ctcatgaccg cagaaatgtt tgttggtgag acaacaaatg
    14761 atagagttag gacattaggt ttgatgcctg gagagaatat tgtagagttt gaacattatt
    14821 ttaaaaatca agtaaatgaa ctgttagact caaatcaaga ggttatcgtt ttgactgact
    14881 tgattggagg aagtcctaat aatgtggctt tgtcacggtt tttaaatttg gattcagttg
    14941 atattgtaac agggtttaat atccctctcc tagtggaatt aatatcaagt tatgattcaa
    15001 aaatcaattt agaagaaatt gttcaaaatg ctcaaaatag tttgtttaat gttaaacaac
    15061 aacttaacgt agaggaggaa gaggatttat gtctatagag tttgttcgta ttgatgaccg
    15121 tctggtacat ggtcaagttg tcactacgtg gctaaaaaag tatgatattg agcaagttat
    15181 cattgttaat gatcgcatct cagaagataa aacacgacaa tctattttaa agatttctgc
    15241 accggtaggt ttaaaaattg ttttctttag tgtaaaacgg tttgtggaag ttttaaactc
    15301 tgtgccaata aaaaagagaa caatgctgat atatacaaat ccaaaagatg tgtatgattc
    15361 tattgaagga aatttaaaat tggagtacct caatgtagga cagatgagta aaacggagga
    15421 aaatgaaaag gtaacgggag gtgtagctct aggtgaagaa gacaaatatt attttaagaa
    15481 aatagttgat aagggaacga gagttgaaat tcaaatggtt cctaatgata aagttacaat
    15541 gttggaaaaa tttttataaa aataatttaa ggaggtacag tatatgctat tcacacaagc
    15601 attactggtg acattagttg ggattattgc cactattgac tataatggac cgttatttat
    15661 gattcaccgt cccttagtta caagtgcaat ggttggctta gtcttaggag atttcaccca
    15721 aggtgttctt attggttcag ctcttgaatt aacttggctc ggtgtaacag gtattggagg
    15781 ttacactcca ccagatacta tttcaggtgc gattattggt actgcatttg gtattttatc
    15841 tggtcaagga gaaactgctg gtatcgctat agcagttcca attgcagttg ctacccaaca
    15901 gttggatgtt cttgcaaaaa ctttagatgt ttattttgtg aaaaaagctg ataatgatgc
    15961 taaaaacgga gattattcaa agatcggttt ttatcattat tcaagtttgg ttttaatcac
    16021 gttatttaaa attgtaccaa ttttcctagc tattatgctt ggaggggaat atgtggcaga
    16081 cttgtttgct aaggttccac caatcgttat gcagggactt aactctgcag gtgctttact
    16141 accttcaatt ggttttggta tgcttttaaa tatgatgctc aagaaaaata tgtgggtatt
    16201 cttgttgatt ggattcattt gttctgtgta tggaggaatg tcaaccattg ggatctcact
    16261 agttggtatt gcggtagcat acttctacga tatgattgga agtaaaccac aagaaacaac
    16321 ttcaagtagt gatgttgagg aggatcttga tctatgatga ataataaagt aactaaagtt
    16381 gaacttaaaa aagttttcaa acgaagtttt atgtatggtt cttcatggaa ctatgagaga
    16441 atgcagaacc taggttttct atatacaatt cttccagtat tgaaaaaact atacccagac
    16501 aaagattcag cttctcctgc aatgaaacgt caccttgagt ttttcaatac tcatcaaaca
    16561 gcggcaccat ttattcttgg agttacttcc gctatggaag aacaagaagg aaatgaaggt
    16621 gcagcttcaa ttactggtat taaagttggc ttgatggggc cactggctgg tctaggagat
    16681 agtttgttct ggctgacact agttcctatc tgttttagta ttggtgcgtc ttattctaaa
    16741 gacggcggtg ctttaggtat ctttatcgcc ttaatattgt ttaatattat taatattcct
    16801 gttaaatatt tcggtttgaa atatgggtat actaagggtt ctagtcttat ccaagaaaat
    16861 aatacaaaag gaacattgaa tcgcgttacg agtatggcga cagcattagg gctagtacta
    16921 gtgggtggtt tgattccatc aatggttggt attaattttg gattagaatt taagcagggg
    16981 gaacttgtta tttctgttca agaaatgatt acaaaattaa ttccaggatt tatccctatg
    17041 gctttgactt tattaatgtg taaattaatt agaaaaggaa agaatccggt tgtactaatc
    17101 tttagtgtta tggctattgg agttatccta gttgttttag gaattttgaa gtagtagaaa
    17161 gtgtggaggt ggtatttggg atatcacctc cattttggaa gagaggtaaa gagtgaaatt
    17221 atggtataag aaagctgccg caaattggaa tgaagccttg ccgattggga acggtcattt
    17281 aggtggtatg atttatggtt cagctacaaa agaatgtatt caactaaacg atgagactat
    17341 ttggtataga ggaaagtcag atagaaataa tccagactca ctattgcatc ttaaaaaaat
    17401 tcgggaatat cttttagatg gagaaattca gaaagccgaa gaattgataa agttaacaat
    17461 gtttgctacc ccaagagatc aaagccacta tgaattactt ggggaacttt acattgagca
    17521 tatagatatt cagtcttgtg ctctttcatt gtatgaaaga gagctagatt tagatacagc
    17581 tatttctaat gttgtgtttg agcctaatag ttgtaattta caaataaaaa gagaatattt
    17641 tacgagtttt aataagaata ttttatgttg ccgtatagtg tcatcagttc aaaacacatt
    17701 aaatttaaac attaatttgg gtagaaataa acggtttaat gacgaagtat ctaaactgga
    17761 ttcaagtaca attttaatgt cggcctctgc tggaggtaga aaaggtgttc agtttaaagt
    17821 agtatgtcat tctaaggtta cggatggtga agtaagtgta ttgggagaga caatagttat
    17881 tcggaatgct acagaggtat ttctttatct caaatcaatg acggattatt ggggaaatat
    17941 agatatttct tctcttcagg gagaatttag tagtattgat tactttacag aaaaagatga
    18001 acatgtaaaa aaatatcagg agcaatttaa tagagttgat tttaaactag actatagtaa
    18061 aggttgtctt agcattccaa cgaatctact tcttgaaaac actaaaaagt atagtaacta
    18121 cttgactaac ttgttatttc attatggaag atatctgtta atatcgtcta gtcaaccgaa
    18181 tggtttacct gccaatcttc aaggaatatg gtgtgatgaa ttaaatccaa tttggggttc
    18241 taaatatacg attaatatta atactcaaat gaattattgg atggtaggtc catgtgattt
    18301 accagaagta gaatatccat tatttgatat gctcgaaaga atgagagaac cgggaagact
    18361 aaccgctaag aaaatgtatg gagctagagg ttttacagca catcataata cggatggttt
    18421 tggcgatacg gctccccaat ctcatgccat gggggctgca atttgggtat taactattcc
    18481 atggttatgt actcatattt gggaacacta tttatatttc caagatgagc gtattcttac
    18541 ggaacatttt gaaatgataa aagaagcatt tcttttcttt gaagattatt tatttgaggt
    18601 ggatggctac ttgatgacag gtccaagtgt ctcaccggaa aataaatatc gcttaaaaaa
    18661 tggtattgaa ggaaatgctt gtctatcatc tacaattgat aatcaaattt taagatattt
    18721 ttgtgattca tgcattggaa ttgcaaaaca attaggagac aattcggatt ttattagtcg
    18781 tgtgaaggag ttaaaaaaga aactacctaa aacaaaaata ggtagtaatg ggcaaatcca
    18841 agaatggtta gaagattatg aagaagtaga gcctgggcat agacacattt cacctctatt
    18901 tgggctttat ccttataatg agattgatat tcataaaact ccggaattag cagaagcagc
    18961 taaaatcact atcaatagga gattatcaaa cgctaacttt ttatcttcac aggagaggga
    19021 gcaagcgatt aataattggt tagtaagtgg tttgcatgct agtacacaaa caggttggag
    19081 tgctgcatgg ctgattcatt tttttgcgag actatatcaa ggtgaacctg cttataacca
    19141 gattaatggt ttgttaaata atgcgactct tggcaattta tttcttgacc atccaccatt
    19201 tcaaattgat ggtaatttag gtttggtgag tggaatttgt gaattattag tacagagcca
    19261 tcataattgg ttatcactaa ttccagcttt accttctgct tggtcagaag gagaagtgaa
    19321 aggtttcaga gtaagaggag gatataaggt atcgtttgct tggaaaaatg gggatataac
    19381 attcctaaaa ttggaaggag gaaacaaaga tcaaaaagta agagtaagaa tatatggcaa
    19441 aaatactgat gtacaaaata ttgaattggt atttaattca gaaaaaatta ttgagttaaa
    19501 tttttaggta taagtcatga ataaagaaaa aataaaaaga aaattaatca caatactgtt
    19561 tgtatgtatt gggatgttat gttttggatt gttagcagga gttaaggctg ataatcgtgt
    19621 tcaaatgaga acgacgatta ataatgaatc gccattgttg ctttctccgt tgtatggcaa
    19681 tgataatggt aacggattat ggtgggggaa cacattgaag ggagcatggg aagctattcc
    19741 tgaagatgta aaaccatatg cagcgattga acttcatcct gcaaaagtct gtaaaccaac
    19801 aagttgtatt ccacgagata cgaaagaatt gagagaatgg tatgtcaaga tgttggagga
    19861 agctcaaagt ctaaacattc cagttttctt ggttattatg tcggctggag agcgtaatac
    19921 agttcctcca gagtggttag atgaacaatt ccaaaagtat agtgtgttaa aaggtgtttt
    19981 aaatattgag aattattgga tttacaataa ccagttagct ccgcatagtg ctaaatattt
    20041 ggaagtttgt gccaaatatg gagcgcattt tatctggcat gatcatgaaa aatggttctg
    20101 ggaaactatt atgaatgatc cgacattctt tgaagcgagt caaaaatatc ataaaaattt
    20161 ggtgttggca actaaaaata cgccaataag agatgatgcg ggtacagatt ctatcgttag
    20221 tggattttgg ttgagtggct tatgtgataa ctggggctca tcaacagata catggaaatg
    20281 gtgggaaaaa cattatacaa acacatttga aactggaaga gctagggata tgagatccta
    20341 tgcatcggaa ccagaatcaa tgattgctat ggaaatgatg aatgtatata ctgggggagg
    20401 cacagtttat aattttgaat gtgccgcgta tacatttatg acaaatgatg taccaactcc
    20461 agcatttact aaaggtatta ttcctttctt tagacatgct atacaaaatc cagctccaag
    20521 taaggaagaa gttgtaaata gaacaaaagc tgtattttgg aatggagaag gtaggattag
    20581 ttcattaaac ggattttatc aaggacttta ttcgaatgat gaaacaatgc ctttatataa
    20641 taatgggaga tatcatattc ttcctgtaat acatgagaaa attgataagg aaaagatttc
    20701 atctatattc cctaatgcaa aaattttgac taaaaatagt gaggaattgt ctagtaaagt
    20761 caactattta aactcgcttt atccaaaact ttatgaagga gatgggtatg ctcagcgtgt
    20821 aggtaattcc tggtatattt ataatagtaa tgctaatatc aataaaaatc agcaagtaat
    20881 gttgcctatg tatactaata atacaaagtc gttatcgtta gatttgacgc cacatactta
    20941 cgctgttgtt aaagaaaatc caaataattt acatatttta ttgaacaatt acaggacaga
    21001 taagacagct atgtgggcat tatcaggaaa ttttgatgca tcaaaaagtt ggaagaaaga
    21061 agaattagag ttagcgaact ggataagcaa aaattattcc atcaatcctg tagataatga
    21121 ctttaggaca acaacactta cattaaaagg gcatactggt cataaacctc agataaatat
    21181 aagtggcgat aaaaatcatt atacttatac agaaaattgg gatgagaata cccatgttta
    21241 taccattacg gttaatcata atggaatggt agagatgtct ataaatactg aagggacagg
    21301 cccagtccct ttcccaacac cagataaatt taatgatggt aatttgaata tagcatatgc
    21361 aaaaccaaca acacaaagtt ctgtagatta caatggagac cctaatagag ctgtggatgg
    21421 taacagaaat ggtaatttta actctggttc ggtaacacac actagggcag ataatccctc
    21481 ttggtgggaa gtcgatttga aaaaaatgga taaagttggg cttgttaaaa tttataatcg
    21541 cacagatgct gagactcaac gtctatctaa ttttgatgtg attctatatg acaataatag
    21601 aaacgaagtt gctaagaaac atgttaataa tttgtcgggt gaatctgtta gtctagattt
    21661 caaagaaaaa ggagcaaggt atattaaagt taaattacta acgagtggag tgcctttgag
    21721 tttagcagaa gtagaggttt ttagagaatc agatggtaag caatctgaag aggatataga
    21781 taaaataaca gaagataaag tagtctctac aaataaggta gctactcaaa gttcaaccaa
    21841 ttatgagggt gtagctgctt tagcagttga tggtaataaa gatggagatt acggacatca
    21901 ttcggtgact catactaagg cagattctaa cgcttggtgg caggtcgatc tgggagaaga
    21961 gtttacggtt tctaaagttg atatttataa tagaacagat gccgaacctc agcgtttatc
    22021 taattttgat gttatttttc tatcttcatc aggagaagaa gtttttagaa gacattttga
    22081 taaagtagtt gatggtttgt tatctttaaa agtaccttct gtaggggcta agctagtcaa
    22141 aatagaatta aaatcagcag ctattccgtt aagtttagcg gaagttgaag tctatggttc
    22201 aaagagaact ccgaagaaac tttctaatat tgcattaacc aaagaaactc gacagagttc
    22261 aacggattac aatggttttt ctcgtctagc agttgatgga aataaaaacg gagattatgg
    22321 tcatcattca gtgactcata ccaaaggaga ttctccttca tggtgggaga tagatttagc
    22381 acaaaccgaa gaattagaaa agttaattat ttataataga acagatgctg aaattcagag
    22441 attatcaaat tttgatatta ttatatatga ttcaaataat catgaagttt ttaaacaaca
    22501 tattgacagt ttagaaagca ataatctatc catagactta aaagggctga agggaaaaaa
    22561 ggttagaatt tctttgagaa acgcaggaat tcctttaagt ttggcagagg tagaggttta
    22621 tacttataag taattttaaa aattatcatc ggtagtatga aagaaacaga aaaataagag
    22681 gaaaatagta tgattcaaca tccacgtatt gggattcgtc cgactattga tggtcgtcgt
    22741 caaggtgtac gcgaatcact tgaagtacaa acaatgaaca tggctaaaag tgtggcagat
    22801 ttgatttcaa gcacattgaa atatccagat ggggaacctg tggaatgcgt gatttctcca
    22861 tctactattg gccgtgtacc agaggctgca gcttcccatg agttgttcaa aaaatcaaat
    22921 gtttgcgcaa caattacagt tacaccatgc tggtgttatg gtagtgaaac tatggatatg
    22981 tctccagata ttcctcatgc tatttgggga tttaatggga cagaacgccc aggagctgtc
    23041 tatcttgcag ctgtactagc ttcacatact caaaaaggga ttccagcctt tgggatttat
    23101 ggtagagatg ttcaggaagc taatgataca gctattccag aagatgtcaa agaaaaactt
    23161 ttacgttatg cgcgggcagc tcttgcaact ggcttgatga gagacactgc ttacctatca
    23221 atgggtagtg tttcgatggg gattggtggt tctattgtaa atccggattt cttccaagaa
    23281 tacttaggaa tgcgaaatga atcggtagat atgacggagt tcacacgccg tatggaccgt
    23341 ggtatttacg accctgaaga gttcgaacgt gcgctcaaat gggtgaaaga aaacgtaaaa
    23401 gaaggattcg accataaccg tgaagacctt gttttaagcc gtgaagaaaa agatagacaa
    23461 tgggaatttg ttattaagat gttcatgatt ggacgtgact taatggttgg taacccaaga
    23521 cttgctgaac ttggttttga ggaagaagca gttggtcacc atgctttagt agctggtttc
    23581 caaggtcaac gtcagtggac agaccatttt ccaaatggag attttatgga aactttcctc
    23641 aatactcagt ttgactggaa tggtattcga aaaccatttg tatttgcgac agagaatgat
    23701 tcactaaatg gtgtgtctat gctctttaat tatctattaa caaatactcc acaaatcttt
    23761 gctgatgtgc gtacttattg gagtccagag gctgttgaac gtgtaacagg atatacttta
    23821 gagggtcgtg ctgcagctgg cttcttacat ctaatcaact ctggttcttg tacattagat
    23881 ggtacaggtc aagctactcg agatggcaaa cctgttatga aaccattctg ggagttggat
    23941 gaaagtgaag tgcaggctat gcttgaaaat acagacttcc caccagcaaa ccgcgaatac
    24001 ttccgtggag gaggattctc aactcgtttc ttgacgaagg gggatatgcc agtaacaatg
    24061 gtacgtctca atcttctaaa aggggttggt ccagtgctac aaattgcaga aggttataca
    24121 cttgaacttc ctgaagatgt tcaccatact ttagataatc gtacagatcc aggatggcca
    24181 actacttggt ttgctccacg tttgacagga aaaggtgctt tcaagtctgt ctatgacgtc
    24241 atgaataatt ggggagctaa tcacggagcc ataacatatg gacacattgg agcagacttg
    24301 attaccttgg cttctatgtt gagaattcct gtcaatatgc ataatgtacc tgaggaagat
    24361 atctttagac ctaaaaattg gtccttattt ggaacagaag atctagaatc agcagactat
    24421 cgtgcatgtc agttgttggg accactacat aaataaaact tgtttatata ggaggtgaac
    24481 ttacgtccct cctatccttt taaaaagatt tgttaaacaa ttcacaaata attgaaaacg
    24541 aatacaaaaa gtaatataat gatgttaaat agatagcgcg gaggcgcagg aggaaaatta
    24601 tatggctaca ttttatgttc cggcagtcaa ccttattgga aaaggtgttg taaatgaagt
    24661 gggtccttat atcaaggaac ttggctataa aaaggcactt ttggtgacag ataagtacat
    24721 cgaaggcagt gatattttac ctaagacttt aaaaccactg gatacagaag gaatcgaata
    24781 tgtcatcttt agcgatgtag agccaaaccc tacttgtaag aatgtcacag atggggtagc
    24841 tgctttgcaa gaacatggat gtgattttat catcagtctt ggcgggggtt ctccacagga
    24901 tgcagctagt tgtatttcta tcatggctac aaatggtgga aaaccacagg attatgaagg
    24961 gcttcataag tctgctaaaa aaggcttgcc agttgtggct atcaatacaa cggcaggaac
    25021 ttctgcagaa atcactatta actatgtgat tactgatgaa gaacgcaagg ttaagatggt
    25081 aatggttgac aagaatagcc ttgctcttat ctctgttaat gacccggaac tcatgctttc
    25141 caaacctaaa ggtctgactg ctgctactgg tatggatgct ctgactcacg ctgttgaagc
    25201 tttggtaaca cctggtgctt atgatgtaac caagaaactg tctattggtg ctattgagct
    25261 tatcaaggaa tatcttcctc gtgctgtaga aaatggacat gatattgaag cgcgtgaagg
    25321 tatggtcaat gccatcttcc ttggtggtat gagctttaat aatgctggtc ttggttatgt
    25381 tcactcaatg gctcaccaac tcggtgcagt atataatttg ccacatggtg tttgctgtgc
    25441 catgttgcta ccagttatag aacgtgaaaa tgctaaacgt gtaccagaag ctttccgcaa
    25501 tgttgccaaa gccttgggcc ttcatgtgga aggtaaatca gatcaagagt gtgccgatta
    25561 tgcgattgct gagattgaga agctttctga gacagtaggt attcctaaga aattgactga
    25621 acttggtatt gaagaaaaag atttcgactt tgaatacctt tctaagaatg ccttgattga
    25681 tgcctgcgca ccaggaaatc catttatgcc aaccttagaa gaaacgattg ccttttataa
    25741 agagttattt tagcaagaga ttagacgaca ataggacaat tcacctctaa ttattgacat
    25801 ttcaggaaaa atcgcagtaa aaacggcaca gccttcttta aatgtgctat aatactagaa
    25861 aaatacttgt ggaggttcca ttatggcaat atttttcatg atttttctga ttgtttgtgt
    25921 gctcctattg gtgatagtca cactgagtac agtttatgtg gttcgtcagc agtcggtggc
    25981 gattattgaa cgctttggga aataccaaaa ggttgctaat agcggtattc atattcgctt
    26041 gccttttggg attgactcga ttgcagcacg gattcagttg cgcttgttgc aaagtgatat
    26101 tgtggttgag actaagacca aggacaatgt gttcgttatg atgaatgtag cgactcagta
    26161 ccgtgtcaac gagcagagcg tgacagatgc ttactataaa ctcatacgtc cagaatctca
    26221 gattaaatct tatatcgaag atgctcttcg ctcttctgtt ccaaaattaa ccttggatga
    26281 attgtttgag aaaaaagatg agattgccct tgaggttcaa caccaagtag cagaagaaat
    26341 gaccacttac ggctacatta tcgtgaaaac cttgattacc aaggtcgaac cagatgcaga
    26401 agttaagcaa tctatgaatg aaatcaatgc ggcgcaacgt aagcgggtcg cagcacaaga
    26461 attggcggaa gctgacaaga ttaaaattgt cactgcagct gaagccgaag cagaaaaaga
    26521 ccgccttcat ggtgtgggga ttgcccaaca acgtaaggcg attgtggatg gattggcaga
    26581 gtctatcacc gaactcaagg aagccaatgt tggcatgaca gaagaacaaa tcatgtctat
    26641 cctcttgacc aaccagtatt tggatacctt gaataccttt gcctctaaag gaaatcaaac
    26701 catcttttta ccaaatactc caaatggtgt ggatgatatc cgtacacaaa tcttgtcagc
    26761 ccttcgcgct gagaagaaat aatagactaa tactcttcga aaatctcttc aaactacgtc
    26821 agcgtcg
//
LOCUS       DAVBNO010000025        26014 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVBNO010000025 DAVBNO010000000
VERSION     DAVBNO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.025"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(15..311)
                     /locus_tag="VQ846_001157"
     CDS             complement(15..311)
                     /locus_tag="VQ846_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075737.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            complement(295..2454)
                     /gene="pfbA"
                     /locus_tag="VQ846_001158"
     CDS             complement(295..2454)
                     /gene="pfbA"
                     /locus_tag="VQ846_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET3075738.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHTIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRENGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SVIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3584..4810)
                     /locus_tag="VQ846_001159"
     CDS             complement(3584..4810)
                     /locus_tag="VQ846_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET3075739.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVFEVGPGDEVIVPAITYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4853..5545)
                     /locus_tag="VQ846_001160"
     CDS             complement(4853..5545)
                     /locus_tag="VQ846_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET3075740.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGNLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSTEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFKVLK"
     gene            complement(5731..7482)
                     /locus_tag="VQ846_001161"
     CDS             complement(5731..7482)
                     /locus_tag="VQ846_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075741.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALVDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(7472..9217)
                     /locus_tag="VQ846_001162"
     CDS             complement(7472..9217)
                     /locus_tag="VQ846_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET3075742.1"
                     /translation="MSIIQKLWWFFKLEKHRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTELRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSEVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9423..9719)
                     /locus_tag="VQ846_001163"
     CDS             complement(9423..9719)
                     /locus_tag="VQ846_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET3075743.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(9872..10328)
                     /locus_tag="VQ846_001164"
                     /pseudo
     CDS             complement(9872..10328)
                     /locus_tag="VQ846_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10461..11288
                     /gene="xth"
                     /locus_tag="VQ846_001165"
     CDS             10461..11288
                     /gene="xth"
                     /locus_tag="VQ846_001165"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET3075744.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAKNADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11300..11443
                     /locus_tag="VQ846_001166"
     CDS             11300..11443
                     /locus_tag="VQ846_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET3075745.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            11443..11682
                     /locus_tag="VQ846_001167"
     CDS             11443..11682
                     /locus_tag="VQ846_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET3075746.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVSKLYQRVMKANYPI
                     YRPLTKRKFRVSDPYIYPHEFAVLDPDGYFLRFSE"
     regulatory      12065..12160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12560..13141
                     /locus_tag="VQ846_001168"
     CDS             12560..13141
                     /locus_tag="VQ846_001168"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET3075747.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGRVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13141..14403
                     /locus_tag="VQ846_001169"
     CDS             13141..14403
                     /locus_tag="VQ846_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET3075748.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(14490..15356)
                     /locus_tag="VQ846_001170"
     CDS             complement(14490..15356)
                     /locus_tag="VQ846_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HET3075749.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(15343..16113)
                     /locus_tag="VQ846_001171"
     CDS             complement(15343..16113)
                     /locus_tag="VQ846_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET3075750.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(16139..16993)
                     /locus_tag="VQ846_001172"
     CDS             complement(16139..16993)
                     /locus_tag="VQ846_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET3075751.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYINNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(17150..17554)
                     /locus_tag="VQ846_001173"
     CDS             complement(17150..17554)
                     /locus_tag="VQ846_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET3075752.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERQVRI"
     gene            complement(17624..19105)
                     /locus_tag="VQ846_001174"
     CDS             complement(17624..19105)
                     /locus_tag="VQ846_001174"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET3075753.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSRKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(19124..20302)
                     /locus_tag="VQ846_001175"
     CDS             complement(19124..20302)
                     /locus_tag="VQ846_001175"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET3075754.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAFDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEVFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            20466..21473
                     /gene="galR"
                     /locus_tag="VQ846_001176"
     CDS             20466..21473
                     /gene="galR"
                     /locus_tag="VQ846_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET3075755.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(21519..22556)
                     /locus_tag="VQ846_001177"
     CDS             complement(21519..22556)
                     /locus_tag="VQ846_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET3075756.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(22557..22910)
                     /locus_tag="VQ846_001178"
     CDS             complement(22557..22910)
                     /locus_tag="VQ846_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET3075757.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(23154..24044)
                     /locus_tag="VQ846_001179"
     CDS             complement(23154..24044)
                     /locus_tag="VQ846_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET3075758.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALSRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            24177..24713
                     /locus_tag="VQ846_001180"
     CDS             24177..24713
                     /locus_tag="VQ846_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET3075759.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(24879..25685)
                     /locus_tag="VQ846_001181"
     CDS             complement(24879..25685)
                     /locus_tag="VQ846_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET3075760.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLPN"
BASE COUNT         7804 a         5737 c         4613 g         7860 t
ORIGIN      
        1 gatttgtcct attattattt cattttacta tactctgtta attcatatga gtttaaaccg
       61 atttcatctt taacctcgag taaagcagtt tcaaatattt gtttaagagt ttttgattct
      121 ttacaattaa ccgacaaact ttctgataaa atatgtacaa cttctgagac tgaataacct
      181 atctcctctt tagaattata taaatctgta gctccaccaa taatccaaaa atactgattt
      241 tgtgaaccta caatatcctc attttctacg gaacttcctt gtatcgaaca aattttattt
      301 atctttacca taatacttca acccttttag tgtcaaaagt aaaccaattc ctgtcactgt
      361 taagaatagt tccataatct tgttcgaacc agtctttggt aatttttgtt ttacatctac
      421 tatttcttta gatttattaa tatgattttc agtttctctg ccatctccaa ctattttata
      481 gtttacttct tctgtcttat tatcttgttt attgtcgatc ttgtcattca tttgtctatt
      541 atctttactt gagttaaact ctccgttctt ctggttacta tcaattacat tatttgaatt
      601 agattgtttt tcctctttgt ttttttcttt ttcgttttta tcacttaaat tatttgttac
      661 aattttgtaa agcccattct ccgttacaat attgaaatta ccatcgctat cacgtataac
      721 aggttctttc ccatttgcat tagatttgat gactgatata tacttaccgg ataaattata
      781 aaattggtta tttaaaacgg ttattttacc ctttgaatcc tcaataacaa ttccttcttt
      841 accaccgaat atactattct ctgagactac taaattatca ctaactcgta ataattcaat
      901 atttggttgt tctgtttcct tagaattatt attaattaca ttttttgtta cagtaatatc
      961 tgatactttt cctaaatatt ctgcactatc ttttgcaact cgtatcgctt ttgttttagg
     1021 atcggcaata ttaaatatat tttcggcgat aaccacctgt ttatttaaat ctaataggtc
     1081 tttagtgttt ttatagctat aagcatttac taaagctgct ccgttttctc gataatgtac
     1141 actctctcct ttaactttct tatcaaagcg atttcctttg attaatacat cagtgaatcc
     1201 tgtaaaacgt acacctgcat acatcatgtt atcaaaatga ttatttaaaa ttttaatatt
     1261 agaggggttc tgtgtcgaca atgtttgata gtgtgtacca attgctgtta ctaattcccc
     1321 agatttatca cttttgccaa aataggaatt ttgaatagtc acattttcag attttttccc
     1381 atcatcattc aaggcataag gaaaaccttt tctagttaat ggttcaatct gaatgctctc
     1441 cttacttatg atttgcccat ccttcatcgt tttgggtaag gcttgcccaa gaaaacgaga
     1501 attatcaact aatacatttt tcgaacctgc aatttgaata gtatgccctt gataactatc
     1561 cttgaatgtt acatttttta tagttacgtt atttgaattc ccaatagcaa atgcacctga
     1621 agaatttata agtggtagat tttttgcttt agttccttct tcattcaaag caccgttcat
     1681 atcaatcgta ccaccagaat aactaatatc ttctgttggg ccccattcta cttgcgcacc
     1741 atcatccgta aataaacctg tcataaaaac aatggaaggg tgattcttaa tatttatacc
     1801 atttagaatt gtagctttct cattcaattc taagtgtgta tgacttttta aaaaaacaat
     1861 ttcttttact aaataagttc cttcaggaaa atatacattt cctccaccta gcccttgagc
     1921 tgcagcatct attgcatctt gaattgcttg tcgatcatca tgaatcccat cacctaccgc
     1981 accataatct tttacactta aagcagtatc ttcaaattct tttttgataa ttctgtcact
     2041 tataacatct tccttataac tcttattttc attagtagat gttactgtag attcaactgt
     2101 tctgttgtct aattgtgaat tggaacgaat agtaccatta gagctgatac tattaacact
     2161 taacgaatta tctaaaccat tagtggttga attagaggca atcatttcag cagaacgatt
     2221 ctgattaaca gttggtgaat tatctaaatc attagtaatt gcattagaag actcttttgt
     2281 agccatcggt gaagaactag taacaacttc atccgataaa ataccttggc ttcccaaaat
     2341 tgaaattgcc aatagtaccg agcaagtccc cacctttaat ttacgaatac tgaatacctc
     2401 attaggaaca aaatatttca tcagaacctc caattttttt actattttta acaattgatt
     2461 attacttttc ttaaaataga tagaatgaca ccgctgtcat tatctttatc atatattttc
     2521 caaattgaat tgtcaaggaa taacacaaga aaatattaaa atttatactc ttcggaaatc
     2581 tcttcaaact aggtcagtgc cgccttgccc taagtctagg ttactgactt ggtcagttct
     2641 atagtaaatt gaaactaaaa tagtacacct ctacttctaa aacattgtta gaaatcgatt
     2701 tgactgtccc gatcaatttg tcctattctt atttcatttt actatatcta caacctcaaa
     2761 gcagggtttt gagctatcta tggccaactt cctagtttgc tctttagcct tccattcaaa
     2821 agtgatgcta tctttcttag cttgaatata aaatatccca tttactatat ttaaatttat
     2881 tgatatgttg taaattataa atgaaagaac aaataccttg tcatacaagc attcgccagg
     2941 gatatttaca tgaatatcgc tcatatggcg gattgaaact aaaacactac aatatatttc
     3001 ttaaatagac cttctataaa agctagattt aacctcgata aaaatagatg cagtgcttct
     3061 aaaatactgg aatagggcat ataggtaata gtagacaagt gttttataga ttgtctcgcg
     3121 ggagatctaa tgatgtatag caagcatcac taaacatata gaatatttgt gttattataa
     3181 tatcaatatg ctataaattc tatcatgcaa aagaatgact agaacatgaa agacaggcac
     3241 aaaactttga acaatattct gaaaacaaaa aaagacgcga gtttccacaa tctcactcat
     3301 ggaaactcac gtctttcaaa aaatagggtg aattaaaaac tagaacaaga cactagattt
     3361 tctaaatgat gtctagtaag gattctaaat gctacaccaa attgctcaca tcttatttca
     3421 aaccacggga ctcccacaag aaaggaaaat cctataaaaa ttctctctct gaggagagag
     3481 aggcctcaac tacaggtgta aactattatc aattgaaaac aacagttttt acactcaatt
     3541 agacttttta gggacaggat cactttcgct tgactgtagt ttgtcatttt tttgataaag
     3601 ttagcacttt ttcagaaact gttttgaaag tctcaatgat atagtctact tcttcatcgc
     3661 ttaatttagt atgaagaggg agggtaattt cattctcaaa gaaggcatag gccttaggat
     3721 agttcgtcat atcaaatcca agattcttat aggctgtcaa gagaggaagc ggtttgtagt
     3781 gaacattact tgcaattcct gctttagcca attcttggat gatgaggttg cgttcttcta
     3841 ggcttgctcc ttctacacgg gtgatgtaga ggtggcgtga agattcgaca gtttcagtct
     3901 tgtgtgccaa aggatggatg cgagaacctg caaaaccact atcatagcgg tccacaatat
     3961 ccttacggcg ttgcaacaaa cttggatagc ggtccaattg taccaaacca agtgaagcca
     4021 tgatatcggt catgttgcac ttataggctg gtgtaacgat atcgtattcc catgacccca
     4081 gttgcatctt ggcaagagca tccttagttt gcccgtgaag ggaaaggatt tggaattcct
     4141 tgtacatctc ttcgtcatca atcactggat tggctttcca agtcgcactt ccaccttctg
     4201 ccgttgtaaa gttcttaacg gcatggaatg agaaggaagt aaagtcagcg atagaaccag
     4261 aaggttgtcc tttataagta gatcccaaag cgtgggcact atcagagaca atgacaatac
     4321 ggttaaaggc cttttgccac ttgcttgaag cggtaaagaa gtcacgtttt ttctccacga
     4381 cttggaacaa acggtcataa tcgcaaacaa tccctgcgag ctctactggg ataatcacct
     4441 tagttttctc agtgatagct tgctcaagca ggtcatagtc catctcaaac gtatctgctt
     4501 ggatatccac catgacaggg gttgctccca cgtgcgtaat gacactacat gaagccgtat
     4561 aggttatggc tggaacgatg acttcatcac caggtcccac ttcaaaaacg cgtaaaatca
     4621 actccagagc ggctgtcgca gagttgagac aaacagtctt aggtgtctgt gtgtaaagag
     4681 acaagcggcg ctccagttct tttgttttag gacctgttgt gatccaacca gaacgcaggg
     4741 tatccgctac ttcagcaatt tctgcttctg tgatatcagg cggtgaaaat ggaatattgt
     4801 aatttggcat tgatttgctc cttttatctg tactcattct cttatgacta ctttatttta
     4861 gtaccttaaa cacggtttga aacatgattt tgatgtcccc aaagaaacta aactctcgga
     4921 gataggcgag gttatagcgc atcttttcag gaagaacatg ctccacatag gcctgatcaa
     4981 ctgacagacc tttctctgtc atttgactga tgatggtgtc ctcatccttg tagttgatgc
     5041 tggctggaga ggtaattcct gcttgcaaga gcaaggttgc catcatttca gtgctatact
     5101 gctctgtata acgtggcact tcaggtcgtg taccgacaaa ggacatctca cctttaagga
     5161 cattgaccaa ctgaggcagt tcgtccaaac ggacacgtcg gatgaaattt ccaaccttgg
     5221 taatacggct atcgttagca gaagtcacca gatttccttt tttatccgca tccgtcacca
     5281 tggtacggaa cttccaaatc ttgaaccgac ggttgtactg ggtcacacgc tcttgcttgt
     5341 aaatcactgg ccccttgcta tccaacttga tccaaatgct caagatgaga aagatgggag
     5401 aggtcagaac cagtaagacc aaggccaaaa cccagtccaa gcaacgcttg aaaatcagcg
     5461 aacccttcct tttagagaca agctggtagt aagactcaac ctcgcttgat ttcatttcca
     5521 caggcaagtc ttcccatttc agcatgctgt tctcctttgt ttttattata ctatttgtca
     5581 acactttttg tcaacacttt tcattatacc acaaaatgga aaaggcggtg aaaaaaatct
     5641 gtaatttgaa ggatttcctt ctagtactca atgaaaatca aagtacagct ttgagattgc
     5701 agataaagct gacatggttt aaagagattt tcaaagagta taggccatgg cccctgcctg
     5761 caaactatac atcttgtgat aggttcctcc cagagccaag agttcctcat gggttccact
     5821 ctcgataatg cgtcccttat ccaagacata gatgcagttg gcgtcttgaa tagtagaaag
     5881 gcggtgagct atagcaatag ttgttcggcc ctgtctcatc ttcgccagag aagcttgaac
     5941 caagctttct gtttcagagt caatattggc tgtcgcttca tccaaaatca ggattttagg
     6001 ctggctggcg actgttctag caaaggcaag aagctggcgt tgcccagtag agaagctcga
     6061 accacgctcg gaaacagggg agtcgtaccc ctgaggaagt tcttgaataa aggaatctgc
     6121 atccacaaag gctgccgcag cctgaacctg ctcatcactg atttcttggt acatggcgat
     6181 attggactta atagttccat gatagaggaa gggttcctgc aagaccaaac cgatgttttt
     6241 tctcagctct tcttgactga aatccctgat atccacatca tccaagagaa ctctccctga
     6301 ctggaattca taaaagcgca tgaggacatt gataatagac gatttccctg aacctgtatg
     6361 acctacaaag gcaatggttt cacccttatt aacagagaaa gaaatgtcat ccagaatcgg
     6421 atgtttaccg tcatatgaga aacacacatg ttcaaagcgg atattgcctt cttggacttt
     6481 ggcttgccca ttttcttgaa gaggttcata ggtcctctcg tctaccaggg caaagacacg
     6541 acctgcagaa accatagacg tttgcagtgt tgaaaagttt tgcgtcacct caatcaaagg
     6601 gtcaaagagg cggttgatgt actggataaa agcatacatg gtcccggccg ttatcccgat
     6661 agaaagacca cgatagccaa aataggccat caagacagca taacctagaa gtttcaacaa
     6721 actcatggca ggtctcaaaa agagggcatc caaggctaca gaacggttgg catagaccaa
     6781 gtgctcttgg ttgatttcat caaattctgc ctgcaggcgc ttctcttgat taaaggcctg
     6841 gattattctg attccctcga tgttttcagc cagcttgcta ttgatatctg acaagagact
     6901 tctggttttc tcaatgattt tcactgattt tttccgatag agattgacca aaaggaaaat
     6961 caaggggaga aagagcaaga ctaatgctgt caaacgaaaa tccaatacca acatggtata
     7021 aagggttgtc agaaagatga aaactgctga gataaagcta gataaaatcc cagaaaacat
     7081 atcactaatc gtctcggtat cgttggtcaa acgagaaacg atagaacctg ctggcgtctt
     7141 gtcaaagtaa gacatgccca gtttctccat attggcaaag gcatcccgac gaatatccct
     7201 aacaatactg taagacacgc gcgcaaagag aagattgccg acatactgaa ctacagtttg
     7261 taggatgtag agaccatagt agaccagcaa aacggtaacg gctagttggt taagattgct
     7321 gagatactgg tcgataaagt gggaagccac gaggggtatg acacttttaa tgaccgtcgt
     7381 cgctaggaga aaactgagtg ccaaaaaggt caggagtcta taaggcttga gataagacat
     7441 caagcgcttc aatacagccc attgttcttg tttattctgc atcttcttct cctttcattt
     7501 ctaactgctg agactggtag gtttgggcat accagccatc catcgctagc aagtcttcgt
     7561 gcctgcctcg ttcgataatt tgaccatttt gtagaactaa aataaaatct gcatggacaa
     7621 cagcactgag gcgatgggca gttataatgg ttgtcttgtc ctttcgcgtc tccttgaggt
     7681 tgtcgataat cgcatactct gtcttggcat ctacggcgga taaggaatca tccaaaatca
     7741 agatatcagg gtttaaaatc atagcccgac tcatagccaa ccgttgcttt tgaccaccag
     7801 aaagactgac tcctttttca ccaatcagcg tatcaaatcc ttgaggcatg tctacaatat
     7861 cttggtaaac tcgggctagc ttagtagctt cctcaacctc tgaaaggggc aagttaggat
     7921 tgccaaagcg gatattgtct aggattgaag tcgcaaagag aaactggtcc tgaggaacat
     7981 agcccatgag actgcgaagt tctgtcaaac gatagtcccg aatatcgtga ccgtttagat
     8041 aaatggcacc cttatccaca tcgtattcac gcaagaggag cttgattaag gacgttttcc
     8101 cagagcctgt ctgcccaacc aagcccagtg tttgcccttt tgccaaacta aagtgaatat
     8161 ccgtcagtgt ttcctcattt tcaaaagcaa agctgtcaat ggcatactcc aaacgcccat
     8221 tttcaatacc atccagagga aactcagggt cttgtacagg agattcctga gacaaaagat
     8281 tttcaatccg ctggtaagaa accttccctc gctgagtagt attaaagagg aagccgatgg
     8341 ccataagagg ccagaccagc atatccaaat agctgataaa ggtgactaga ttaccaactg
     8401 taatctgccc ttcctgaacc atcaaggagc caaccaaaag cgttaaaaca taggacgaac
     8461 caacaaacaa gagaaccata gggtcaaaga gactatcata tttcatggtt tgcaggttct
     8521 tttggaaggt taattcattg actgcctgaa aagacttcaa ctcgtctgcc tgataaccga
     8581 aagacttggt cactttgata cctgatacgg actcctgtac cttgttattg agttcagaaa
     8641 aagcagcttg ggattcgcca aaggccttat gagtctttct ccctaggcga ctagtcgcat
     8701 aggccatgaa aggtaggggg agaatggcaa caagagtcat ctgccatgag atgctaaaga
     8761 gcatggtcaa caaagtcacc agagccgtga tagaggcatc caccgcagac atgacaccgc
     8821 cacctgctaa acgagtcaag gcattgatat cattggttgc gtgtgccatc agatcacccg
     8881 tccgataggt ttgataaaag gctggcgaca tttttgtgaa atgcttaaac aagcgagacc
     8941 gcatgatctg tcccaagcaa taagaggtcc caaggatata catacgccac acatagcgca
     9001 aatagtacat accaaaggct gcaagtagca agtaaaatag gctaagaagg aggtcctgct
     9061 gggttaattg ccccgatgtg atggcatcaa tgactcgccc cataaccata ggaggaatga
     9121 gattgagaac ggaaaccaag accagggcca caatcccgac tagataacgg tgtttttcta
     9181 acttgaaaaa ccaccagagt ttttgaataa tggacataaa atccctttct gattgcaaat
     9241 ggaaacctga ggccaatact caatgaaaat caaagagcaa actaggaaac tagccgcagg
     9301 ctgctcaaag cactgctttg aggttgtaga tagaactgac gaagtcagta acctacatac
     9361 ggcaaggcga cgttgacgcg gtttgaagag attttcgaag agtacaagac cccaggtttt
     9421 tcttattcat aagttacgac tgtaacagca cccttgtcat attcagcaat aaagatattg
     9481 gctacattgt catgcccttg tttgctgagg ttatcaagca accactcttc gctacgacca
     9541 atcgattcca agacgtctac ttgaatcaca ccgtcagtca caactggata cttaggattt
     9601 tcatctccca tttgcacaac gatgagttgc ccattttgct cttgcacagc tcgtttgact
     9661 tgcttcatct ggaaaatccc ttggctacga agtttgaggg ctacatccga tgcagacaaa
     9721 ccaactgaac gacaggctta tgggtcaata ttcccatttt tgataaggag agttggctta
     9781 ccatcaatca aacgtttcac aaagtgaaca ttgttgttga gctatttgag agtcaagatc
     9841 agtttgatta aaatttcgat ataattaagt gtcatttgtt cacatccacc agttcaattg
     9901 cgtctgtctt atacaattcg actttctcta acaggtactt gtctggctca cttccgttta
     9961 gggcacgata atagcgatct cccaccttga taagtgcgcc atctgtgttt gtggaagtat
    10021 taacatggac ttctgacttg tctactccca aatctttgga aacaacctcg atgaaatgaa
    10081 gtgaagtttg aaatttattg ttagaaactt gattgttttg ataagtcgtg atgctgacca
    10141 aaagcatagc taaataaggc cagagctgaa atcatgacca actcacgaaa tttggttcct
    10201 tttttatcat ggtatgcctt gaaagcaaaa aaaactgtca cagctaatag aacaatccca
    10261 aagccaacca tgataccatt ttgctgaccg atttggctga gtacatagtc ataagagtag
    10321 aatttcatgt attttcactc cctttcataa aatcattctt aattataaac gaaagagagt
    10381 aatttttcaa accatacgtt ggggaaatag ctcttttttt ggtataataa aatctataat
    10441 ctgaatgaaa aggtaacttt atgaaactta tctcatggaa tattgattcc ctaaacgctg
    10501 ccctaactag tgactcagct cgtgccaaat tgtcccaaga agtcctacaa accttggtcg
    10561 ctaaaaatgc tgatattatt gctatccaag aaaccaagct ttctgccaaa ggacctacaa
    10621 agaaacacgt ggaaatttta gaagaactct tcccaggcta cgaaaacacg tggcgttctt
    10681 cccaagagcc tgcccgtaaa ggctatgctg gaaccatgtt tctttataag aaagaactta
    10741 cacctactat cagcttccca gaaatcggtg ccccttctac catggacttg gaaggtcgta
    10801 tcatcactct agaatttgat gcatttttcg taacccaagt ttacactcca aacgctggtg
    10861 acggtctcaa acgcttggaa gaacgccaag tctgggatgc caaatatgct gagtatttgg
    10921 ctgaactaga caaagaaaaa ccagtccttg cgaccggtga ctacaacgta gcccacaatg
    10981 aaatcgacct tgcaaatcct gctagcaacc gccgttcacc tggatttact gacgaggaac
    11041 gtgctggatt taccaacctt ttggcaactg gatttacaga caccttccgc catgttcatg
    11101 gcgatgttcc tgaacgctac acttggtggg cacaacgcag caaaacttct aaaatcaaca
    11161 atacaggctg gagaatcgac tactggctca caagtaaccg tatcgctgac aaggtcacta
    11221 aatctgatat gattgactca ggtgctcgcc aagaccatac accgattgtc ttggaaattg
    11281 atttgtaagg attttctata tggactacaa tgcggtcatt cccgagttgc ttgtatcgaa
    11341 tatcgaacag tcccggtcct tctactgtgg tttactgggt tttaggattg aataccaacg
    11401 tccagaagaa aactttctct ttctcttgaa gagtgtcaac taatgttaga ggagggaaca
    11461 aaggatcaac tagctgaact gacctatcca tttggtcgtg gagtcaatct ctcctttggc
    11521 ataaaggatg tttcaaaact ctatcaaaga gtgatgaagg caaactatcc tatttatcgt
    11581 cccttgacca aaagaaagtt tcgtgtcagt gatccctata tctatcctca tgaatttgca
    11641 gttttggatc cagatggcta ttttttaaga tttagcgagt agaataataa aaattgggaa
    11701 aagtctttaa agttagaaaa aggcatattg tcaggtgttt aaaaacttga tagtatgcct
    11761 tttatgatgg aaaaatcttc taatactctt cgaaaatctc ttcaagccac atcaacgtca
    11821 ccttaccata ggtatatgat actgactctg tcaattctat ccacaacctc aaaacagtgt
    11881 tttgaggttg tggatagctt cctagtttgc tctttgattt tcatttagta taaatgttag
    11941 cttacaatgg agtttttgtt caccccctct tctgtttgtt cgttttttaa atcaaaacac
    12001 taacaaaata gttgatttat aaagttattt agtgtaaaat aaaaacattg gctcaagcct
    12061 atttaaaatt gaatatcgtt ttacttgttt atgtcgtgaa ttggcacgac gtttctacaa
    12121 ggtgccggaa cacctaacaa taagtaagtc agcagtgaga tatggccgtt tttgccatgc
    12181 ctatttgggg gacttactta gagattaagt aggtcttttt tgaatatttt taatatttcc
    12241 cttaattagt gcggacggga ggtgaaatta tcctgtgaat aatttcagga atccagggaa
    12301 tttcatccct aatttttgag cccctagctc tttcatttct aggcttaggc taaaatagtt
    12361 ccccgaacta ttttactctc aaaaatcccg tcatagacaa taacaagatt attgtctatg
    12421 ataccactac aacgggaaat atttgataag actcgcttcg ctcgtaatag ttttatcaaa
    12481 tatatagtat tagcaaaagg gatcactata gaaatattta aacaacaagt aaattaactt
    12541 ttagacttta ggaggtctta tgaaattatt agaagagcgc atcctcaagg atgggcatat
    12601 cttgggtgat aacatcctca aggtagattc ctttttaacc caccaagttg actttagctt
    12661 gatgcgagag attggtaggg tttttgcgga aaaatttgct gctactggca ttaccaaggt
    12721 cgtaaccatt gaagcgtcgg gtattgcccc agccgttttt acagctgaag ccttaaacgt
    12781 tcccatgatt ttcgccaaaa aagctaagaa catcaccatg aacgaaggca tcttaactgc
    12841 tcaagtctac tcctttacca aacaggtgac cagcaccgtt tctatcgctg gaaaattcct
    12901 ctcaccagag gacaaggttt tgattatcga cgatttcctt gctaatggcc aagctgctaa
    12961 aggcttgatt caaatcatcg aacaggccgg tgccacagtc caagctatcg gtatcgtgat
    13021 tgagaaatcc ttccaagatg gccgtgattt gcttgaaaaa gcaggctacc ctgtcctatc
    13081 acttgctcgt ttggatcgtt ttgaaaatgg tcaggtcgta tttaaggagg cagatctcta
    13141 atgcaaactc aagaaaaaca ctcgcaagca gccgttcttg gcttgcagca cttactagcc
    13201 atgtactcag gatctatcct ggttcccatc atgattgcga cagcccttgg ctattcagct
    13261 gagcagttga cctacctgat ttctacagat atcttcatgt gtggggtggc aaccttcctc
    13321 caactccaac tcaacaaata ctttgggatt ggactcccag tcgttcttgg agttgcattc
    13381 cagtcggtcg ctcccttgat tatgattggg caaagccatg gtagtggcgc tatgtttggt
    13441 gcccttatcg catctgggat ttacgtggtt cttgtttcag gcatcttctc aaaagtagcc
    13501 aatctcttcc catctatcgt aacaggatct gttattacca cgattggttt aaccttgatc
    13561 cctgtcgcta ttggaaatat gggaaataac gttccagagc caactggtca aagtctcttg
    13621 cttgcagcta ttactgttct gattatcctc ttgatcaaca tctttaccaa aggatttatc
    13681 aagtctatct ctattttgat tggtctggtt gttggaactg ccattgctgc tactatgggc
    13741 ttggtggact tctctcctgt tgcggtagct ccacttgtcc atgtcccaac tccactctac
    13801 tttgggatgc caacctttga aatctcatct attgtcatga tgtgtattat cgcaacggtg
    13861 tctatggttg agtcaactgg tgtttatcta gccttgtctg atatcacaaa agatccaatc
    13921 gacagcacgc gccttcgcaa cggttaccgc gcagaaggtt tggccgtact tctcggagga
    13981 atctttaaca ccttccctta caccggattt tcacaaaacg ttggtttggt taaattgtca
    14041 ggcatcaaaa aacgcctgcc aatctactac gcagctggtt tcctggttct ccttggactg
    14101 cttcctaagt ttggtgccct tgcccaaatc attccaagct ccgtcctcgg cggtgccatg
    14161 ctggtgatgt ttggttttgt atctattcaa gggatgcaaa tcctcgcccg agttgacttt
    14221 gctaacaatg aacacaactt ccttatcgca gctgtttcaa tcgctgcagg tgtcggtctc
    14281 aacaacagta atctctttgt cagcatgccg acagccttcc aaatgttctt ctcaaacgga
    14341 atcgtcgtag ccagcctact cgccattgtc ctcaatgccg tattaaatca taaaaagaaa
    14401 taagaaaaag aggtgcgagc ctcttttttg tttatcacaa attgattata aatacaatca
    14461 atcccaattc ccatctaatt gaattggacc tatttcaaat tctttaaaat attatgttcc
    14521 aaatcgttaa tattatataa gaatggtaaa atatcaaatg tttctcgtaa attattctta
    14581 gccttatacc aaccttgtcc atcagtaatc cacataaact caacattagg aatagcttta
    14641 atttcttcag caatcatttt gtaagatcta gccgtctcat ttaattttga accacttcct
    14701 gaataaaaat taacctcaat cagataaagc acttgttcat tttttatgac aaaatcaaaa
    14761 cgtttcacag tattcccatc attcgtaata gcagataaat cgacagaaaa aatttcctct
    14821 atttcatttt gttcaatctc tttaaataaa ttctctccta aaatataccc ttcagcttca
    14881 agatagcttt gaacaatatt ttccatcgca tctccagtcc gatttttgcg cccattagta
    14941 tccataccaa cctcaacacc tgtaacgtag tcaactaaat ttgatactaa atgattttgc
    15001 aataaatcaa atatacctga tttttccaaa aacatagtat attcttcaat actatagttt
    15061 cgtttactaa aatcaaaata gaaatctttt atcggatcct taacaataat cgtatctctc
    15121 aaacgtttag caataagtag aggcacaact ttcaaaattt ctggataatt ttgatataag
    15181 tctaaaaaat cctcttgtat atttttacta ccaataagac tattcataat atttagagca
    15241 acttttatac tagatacatt tttataaacc ttaggaaaat ctgtataata agtccagtca
    15301 gctactgtgt cggtcatcgt tgacaaccac tcatcaaaat ttctagtttg tttcattttc
    15361 tgcctccaac ctcttccttg caatttttaa atattctttt tcagcatcga tacctataaa
    15421 tcttctacct aaccgcttcg caacaacacc cgtagtgcca ctaccaacaa atgggtctag
    15481 aatatagtca ccctctttag tagaggctaa aataatgcgt tctaacaaat actctggttt
    15541 ttgagttgga tgtttcccag cccatttttc aactttcttt gttaaagaac cggtccagac
    15601 atctttcatt tgttttccat cattcaattc tttcattaaa tcataattgt agtaatgacg
    15661 agctttttta tcattttttc tggcccataa aatggtttca gtagaatggg taaaataacg
    15721 acaagataaa ttgggggcag ggtttgtttt ctgccaagta atattattca gaattttaaa
    15781 accttcttgt tctaatgcca ttccaactga gtatatgttg tgcaaactac ctgaaatcca
    15841 taccgtccca ttaggcttca gaacttcttt tgctaggcga atccatttac gattaaactc
    15901 atgtttttct tcgaatgaag aaattttatc ccaatctcct ttatcaacag aaacaacttg
    15961 tcccccagaa ttagatattc caccattact taaaaaataa ggtggatcag caaaaatcat
    16021 atccatactt tctggtttca tttttgataa gaacttgaac gtatctgaat gaacaagaat
    16081 catcttattt ttattgtagt atggttttgt cataagaaca cctccatact tatattcgtt
    16141 atttttcata atttgtgact ataatttcag aaatttttcc tcgacttgaa gattttgctc
    16201 cattagttcg ggtagcttca acataatgta tattaaaatc cttatacaac tcctctacta
    16261 aagcactaga agaatttgat aacataacat aagctcctgt atcactcaat ctcttaaagg
    16321 catctcttaa tcttacttga tctgcaaaag agaatccctc atgagtataa gacgtaaatg
    16381 cactcgtctc agacaatgga atatatggag ggtcaaaata cacaaaatct cctgttcgaa
    16441 catctacaat tgccttttca aaatctccca ctttaatttc tagttgattg ttatttatat
    16501 aaactgaaat agcagatatc aattcctcat caacaatttt aggattctta taacgtccat
    16561 atggaacatt aaattgattc ttagaattca cacgatatag accattaaag ttcactctca
    16621 acatatatag aatacgtgca gctctttgta cttcggacat catatctatt ctttcatcac
    16681 gatctgcaga acgtaaatct aaataatatt cttttgaatt atattcctga tgaactttca
    16741 aaatttcaat caattcttga ggattgtcct taatttgttg atagcaattt attagttcag
    16801 cgttaaaatc attaataact gcatctttag gagccaaatc aaaaaataaa gctccacctc
    16861 caacaaaagg ttcgaaatac ctgttatagg ttttaggcat taattctcta ataacaggca
    16921 ataattgtct tttaccacct gtccatttcg tgaacggttg taaagtaact ttctttattt
    16981 cttttatctt catgctttta attataccat agaaattaaa acacaactct tgtattttct
    17041 accacattac taaattttct attttagaat taaatcatat caatatcaaa aaatgagtaa
    17101 ggcaaatctt ataaattcca aaattcacct cactctatta aattactctt tatatcctta
    17161 cctgtctttc ttctgaccgc tggcctgtga caaacatggt aaaggttgct ttacagacat
    17221 ttctgccctc ttgattggtg atatccacat ccatcacaca ggttgtacga ccttgatgga
    17281 cacattctcc tttaatggtc aatacatcgt cgagttttcc tgccttaagg tagttgatag
    17341 aggattggag tgtcacccca tcaagtccca gcgagataac caccaaacca ctgatttggt
    17401 cacatagcgt gaatagatag ccaccatggg cattgccata gtagttgagc gacgagttca
    17461 ctactttggt cgtcaccaca acgtgaccat ctctcatttt ttcaatttcg taattttcaa
    17521 aggcagatat agcgtcaaaa tgaaagtctt tcatcgtttc ctcctgatat ttcaaacgac
    17581 actatgatac tactttttat aggactgtgc aaggagaaag ctcctagtct agtaaaattc
    17641 cgacctgttc cacaaagcgc ataaaggctg tctgaccttg ttctgtctgc ttgtagactc
    17701 ctgcatcctc aagtacacgc acaaagatag cacccacaga gtccttgacg attgcaaggg
    17761 ctttttcttt atccgttagg tctggatgtt gggatttgag ttggtctgcc cactcctgat
    17821 gataatcggc aactgtaaca gcttctccta caagatagct agcgacttgc tccacttctt
    17881 ctttcagacg tggtggcaag attgccaagc ccatgacctc aatcaagccg atattttcct
    17941 tcttgatatg ttggacatcc ttgtggggat gatagatgcc atcaggatgc tctgctgaag
    18001 tctgattgtc tcgcaagacc aagtccaact caaactgtcc atcacgctta cgcgctattg
    18061 gtgtgatggt gtgatgcggt gtcccgtctg tctctgctaa aatctgcaca ctagaatctg
    18121 aatactgacg ccattcctgg aaaatcttat cagctaagtt aatcaaatcc tctttggaat
    18181 ccgaagtcaa gcgtaggaca gacattggcc acttgataat tccagccttg acctgctcaa
    18241 aaccagcaaa tcggaaggtc ttttgcaagg gagccaattc cataggaaat acgtgacggc
    18301 ctccctgata atgatcatga gttagaatag agccccccac aatcggcagg tcggcattag
    18361 atccagcaaa atatcctgga aactgctcta cgatagccaa cagacgttca aaactctgac
    18421 gactaatggc catgggacga tgctggccat ctaaaaagat acaatgctca ttaaagtaag
    18481 catagggcga atactggaac ccccattcct gaccaaccat ttcaaaacgg ataatccggt
    18541 ggttgctgcg agctgggtgg ttgacccgac catggtaacc ctcattctct aaacaaagtt
    18601 gacactgagg ataattacta gcttgcacca acttggctac cgcaatctct ttcggatcct
    18661 tttcaggctt agagagattg atggtaattt caagttctcc ataatcagat ggaacacgat
    18721 aagcgatatt tttagcaatg gccttgagtt tgatgtagtc atttttccga ctgagttgat
    18781 aaaaatcctc tatcgcttgc tcaggagagt aggcataggc ttcccagaag tcacgattga
    18841 cctgactcgg acaaggagtc accaagtcca tcagttcagt accaaggatt tcacgcgcag
    18901 tctgactatc ctcaatcgtc tctaatcgaa cggcttcctc aactagctgg tccttgaggt
    18961 caatcaattt atccagattg gtctcaactt ccaaaacacc ttctcccact cgtgccaaaa
    19021 cacgattggt caggtagatt cgatccattt cctcaaatga actttcagaa atgacatgtg
    19081 ttacaaattt atctactaag gtcactatag agcctccttt tgactagtca aggacgcgag
    19141 tgccacctgc aacttcagcg atatagaagc ttggagcgta tccaactact tcctcgtagt
    19201 gtttgcctac agcttcctta aagacctcaa cagtatcttt ttgaaccaag gcaatggcac
    19261 agccaccaaa accagcccct gtcatacgag caccgagaac tccttcttgt gcccaagctg
    19321 tgtgaacaag ggtatctaat tccaaaccag ttacttcata atcatgctcc agagaaacgt
    19381 gtgacgcatt catcaaacga ccaaatgttt ccaaatcacc tgcttgaagg gctgcttgag
    19441 ctttaagggt gcgttggttt tcaagcacag catggcgagc acgtttcaaa cgattttcat
    19501 ctttaatcag atagctatat tggtcaaagg cccactcgtc caattcaccc aaggtctgaa
    19561 tatccaaggc aacttgcaac tcttccactg ctttttcaca ctcagcacga cgttcattgt
    19621 atttagagtc cgccaattca cggcgtttgt tggtgttcat gataacaacg acattgtcct
    19681 tcaaatcaag tggcaccaaa tcatactcta aggtgttggt atcaaggtaa atagcacgtt
    19741 gatcagcccc cataccaata gcaaactggt ccatgatacc agagttgact ccgataaagt
    19801 tgttttctgt ttgttttccg attttaacca aatcgagacg ctctaatttt aaatcaaaga
    19861 gatgctctgc cacgactcct gtcaagagct ccaaggatgc tgaagaagac aaaccagcac
    19921 catttggtat attcccataa acataaaaat caaaaccttt gtcaatcacg tgcccagctt
    19981 cttgcaagaa atgaaggaca ccttttggat aattggtcca gttgtgctct ttttcaaact
    20041 tgaggtcagc gagaggcact tcgataatgc ccttgtcctc aaagttagct gagtagaaac
    20101 gcaaaacttg gtcgtcacgc ttacgagctg caccgtaagt tcccaaggaa atagcagcag
    20161 gaaaaacgtg cccaccgttg tagtctgtgt gttcaccaat caaattgata cgacctggtg
    20221 aaaagaaagt ttggtctgct tcttgaccaa aaacagcaag aaagtctttg cgaagagttt
    20281 cagcagtaag atgttgtgtc atatgatttc tcctttgact gtccgttaag ccaccttaac
    20341 gtgtaatcgt tttcttctat tgtatccaag ggatttccca aggtcaatcc tttttactaa
    20401 aattttacta aaatatcagt aaaaagcaga aaaatatgat atactaacct aatcaaggag
    20461 gatttatggc taccttaaaa gacattgcac agctagcctc tgtctctatc gcgaccgtat
    20521 cccgtgtcct caaccgcgac cagagcctat ctgttacaga agaaaccaga caccgtattt
    20581 taaccgttgc tgaagagctg ggctacacca agcacctcaa gacaggcgag tcccataaac
    20641 ccaagcaaaa gattgccatt atccaatggg tcagcgaaca aggggagctg gacgacctct
    20701 actactacca gattcgccta ggaatagaaa aaagagccca agagttggac tatgatatct
    20761 tgcgctattt taatgaccat ccttttacct taagcgagga agtgattggg attctctgca
    20821 ttggaaaatt tagccgagct cagatttctg cctttgaaga ataccaaaaa cccctggtct
    20881 ttctagacag tgatacactt tccctgggac atacctgtat tatcacggat ttttacactg
    20941 ctatgaaaca ggttgtcgat tatttcctca gccaaggaat ggaccgtatc gggattctaa
    21001 caggccttga agaaacgact gaccaagaag aaatcattca ggataagcgg ctggaaaatt
    21061 tcagaaacta cagtcaagca aaaggaatct atcatgatga actggtcttt caaggaaact
    21121 ttactgccca gtctggctat gacttgatga aggaagccat tcagagcttg ggagaccaac
    21181 tcccaccagc atttttcgca gccagcgata gtttagccat cggtgccctt cgtgcgctcc
    21241 aagaagctgg aatcagccta ccagaccgtg tcagcctcat ttcctttaac gacaccagcc
    21301 tgaccaagca ggtttatcct cccctctcca gcatcacagt ttatactgaa gaaatggggc
    21361 gagcaggtat ggatattctt aacaaggaag tcctccacgg tcgcaaaatt cctagcctga
    21421 ccatgctggg aaccagactg acattaagag aaagtaccct aaatcaagaa taggataaca
    21481 taaaaaacga atagagttct aaaactccta ttcgtttttt attcgattac aatcatagac
    21541 ttaattgtct tacgttcatc catatcttta taggcttggt cgatatcttc cagtttataa
    21601 cttgaagtaa agacgcgacc tggattgata tcaccatcaa ggacggcttt tagtaaaaat
    21661 tgcttatcgt atgttgtagc agaagctgcc ccacctgcta cagagatatt ttgcataaat
    21721 gtcgaaccaa gagcacgatt attatagtgt gggactccta caaagcccat acgccctcca
    21781 ttatgaagaa cacctagcgc ctgttctata gcagcctccg taccaacaca ttcaagtgct
    21841 gcatctgctc ctccaccgag gatttcacgc accttggtaa ttccttcttg accacgttct
    21901 gcaacaacag ctgtcgcacc tgactccata gccatctttt gacggtcttc atgacggctc
    21961 ataaggataa tttgtgatgc tccacgcatc ttagccgcga tgacagcaca ttgaccaaca
    22021 gccccatcac cgataacaac aaccttgtcc cctttttgaa catttgcaac acgcgccgca
    22081 tgatagcctg tcggcatgac atctgcaaga gtcaaaaggg acttgagcat cccttctgta
    22141 tagtcagaag gttgaccagg gattttaacc agcgcccagt ttgcatagtg gaagcgaata
    22201 tattctgcct gaaaatcacc ccccaaatta ttgccaatat gattgtcgca agaaccgtca
    22261 aatccagcaa gacaggcatc acactcacca catccatgtg taaaagggac aatcacaaaa
    22321 tcacctggtt tcaccgtcgt aatggcttcc ccagcttctt caacaatccc aatcgcttcg
    22381 tgtccactat ttttgtgtcc agctttcgtt tctggattac ggtacctcca taaatctgaa
    22441 ccacaaacgc acgcacgaac cacacgaata atcacatcat ccgcttctat tatttgcgga
    22501 cgttcaatgc tagcaagtcc aacctgacct gcctttgtat atactgctga tttcatttaa
    22561 aattttcctt ccttataaag tttaattttg agatttaaac gatttaaagc tgtctgtagc
    22621 tgagacaagc gctcctctaa tttttgcttt tcctcttcta aaataccaag cctctcctct
    22681 ctcgtttcat ctcccttttg gtagagcgac atatagtcaa ctaaactatc tacagagaca
    22741 cccgccgaac gaaaacactt aataaattcc agcgcttcga tatcctgatc ttgaaaatca
    22801 cgaatcccag tagcagtacg agtaatcggt ggcacaagac caacccgttc ataataccga
    22861 atcgtatccg ctgaaattcc caacaaatca ctggcagatt taatattcac acgctaacct
    22921 cctctcattt cattcactga attcacgcta ttctaactca cttatcttat tatataactt
    22981 tgagttgact ccaagtcaag cattttgtat ctaatcgaaa aatttttatc attaacaatc
    23041 atatatctgg agtttataca atgctcttct cttaccaatt tttgaaatat ttaaataata
    23101 aagaaattac tctattccaa ataagatagt acccaagtat ttttcacttt tttctaatgt
    23161 tgatgctcat aactccgttc caagtcacac acctttcgct tatgggtttg gtgagtttct
    23221 aggtcagcat caatttcaat ggtaatattt tgaaaaccac aatcttttag gaaaattcga
    23281 atagactctt tacaagtttc catatgttcc atttctttta gacaaacatg gacaatggca
    23341 tttttttcca aagcatccat agtccagaga ttaagctgat taaggctggc cacattgtcc
    23401 aatcgttcca ggccactctt tacttgcttg atatcaagac cttctggcac agcatccaag
    23461 aaaatcttga gtgtagacca aaaacgtgaa agggcttttg aaagaataaa gaaagaaatg
    23521 acaagggaca aaagaggatc taggatatac cagtccgtaa atcgaagaac aatcgccatc
    23581 aggataacag ctacccaccc tagcgtatct tccagaaaat gcagactcag aatagactca
    23641 ttctttgtct ttcccttacc aaccaccaga ctcgctaaca gattgatagt aatcgcaata
    23701 attcctaacc agagaatccc ctcatcattg actggttgcg gatgcaaaat cttcgtgaca
    23761 ttttccaaaa tgactagaac agagcctgtt acgagaatca cagctgttac caaggctcct
    23821 agcaggctaa accgcttata gcccaaggtg tactgattgt cttcttcacg attggagatt
    23881 gtttctagaa aagctgatat tccaattgca atcacatctc ccaagtcatg cacagagtca
    23941 gcaagaacag cgctagaacc aaatactcca cctgcaataa actcaacaat ggcataagtc
    24001 aaatttaaga aaaaagccac ccaaacagca tattttgcct tcatatttct cattcctttg
    24061 ttataatagg tttatgaaca ccttgttcat tatcattatc cactaaaact caaagaaagg
    24121 atagaagcaa aacgtcagct ttattcagtt ctgaacaatt tgccttaagt gtccatatga
    24181 ctaacattga ccgccgtatc agcaaaacca aaaaagccat ctatcaagct tttatacaac
    24241 ttttgaatgc taagggctac gaggccacta ctgttcagga tatcattgat ctcgcagatg
    24301 tgggaagatc caccttttac tgtcactatg aaagtaagga gcttcttctg gaccagctct
    24361 gccgctacct cttccatcat ctttttgaaa gagagcaagc catttcaacc gaggactacc
    24421 tcgcccacct ctttctccat tttcataaaa accaagacca tatcaccagt ctgctatttt
    24481 ccaaaaatga ctacttcctc cgtcaactcc ataaagagct agaacaccat gtctattccg
    24541 tgctagctga taatttaaaa gaagcccacc ctaatatgcc tacttcttac ctccaacact
    24601 tggtcgtgtc caactttatc gagacattga cctggtggct caaaaaaggt caagatttca
    24661 cagaccagga agttgtccaa ttttatctag accttctcat tcctaaaaat tgaatataga
    24721 gtaaagctca gttgtcttat ttctaggtta ctgagttttt tatcttttca acaacaaaag
    24781 aggacccgcc gatcctcttt ttcatactat aaatccttga ttatcaacta tatctgtttt
    24841 aatcgaaatc tcaaaacagc actttcaaac atcttttcct agttaggtaa atcagtattc
    24901 tgcttagctg ccttgctcca ttgataccaa ccaactagac tgttaatgag ataaattaga
    24961 tatttccctt gaatttgcag gctttctccc caccagagat agattgaaaa aacattggta
    25021 gccgcccaga atatccactg ttcacggtaa acagctgtca tgaggatttg ccctacccca
    25081 ttggttgcat ctgtgattga atcacgatag ggacgattgg caccaataga ctgataaatg
    25141 aagccaaagg ccaaccacca aagcacacta atggaaagat actttgtcca gcccttgccg
    25201 tccagtttac gcgcgacaaa ctcctgcttt tccttcttaa actgtgcctg ataaatccaa
    25261 actagaagtc caattggctg catgactgtg aagtaaagtg tcgtcagcac ctcaccataa
    25321 aagcctttct gtagggccaa aataaggtaa ataacagagt taatcaagcc aaaaagataa
    25381 ttacttgctc gaccttccga tacaaagatt acacagataa tccctgtcaa gctacaaatc
    25441 atcccaatcc agtcaacaat acgatgttcg taaaccaact ccagccagag aggaaaactt
    25501 cctaaaacca gcaaataaat ccactgggca aaactacgat gggcaaagag gtcatcccag
    25561 atagccttca tagttcctga aaatcctaaa tcagccatag ccgcaaccat acgacggtaa
    25621 ccacctgaca tttcacctag ggttgttttg atattttcaa ttttcttttg caaataagta
    25681 tgcatcattt ctccttttgt ttttaaagag ccgtgtctgg atagactttc ggacgcaacg
    25741 ctctattaga taatgaactg cctatacaca agatttctaa ccttagtcga catgagctga
    25801 aacctcttat ttgttaagta gttcacaaaa tattatacac ctattttatg aatagtcaac
    25861 tgtctttaca gtaaaatttt agaaaatcat gaaaattttc tctttctttc cattttaagt
    25921 gacattcagt cattctcaca tcaaaaaagc ccagacgaaa ctgtctgagc attcttttat
    25981 ctagtcgttt aaggaagttg agttcagtat gttt
//
LOCUS       DAVBNO010000026        23896 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVBNO010000026 DAVBNO010000000
VERSION     DAVBNO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.026"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(65..1051)
                     /locus_tag="VQ846_001182"
     CDS             complement(65..1051)
                     /locus_tag="VQ846_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET3075761.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(1061..1861)
                     /locus_tag="VQ846_001183"
     CDS             complement(1061..1861)
                     /locus_tag="VQ846_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET3075762.1"
                     /translation="MLPYPFSFFSSILGFRKPLSKRFGLNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     PSQIKELPKNLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(2122..2964)
                     /locus_tag="VQ846_001184"
     CDS             complement(2122..2964)
                     /locus_tag="VQ846_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET3075763.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(2966..4258)
                     /locus_tag="VQ846_001185"
     CDS             complement(2966..4258)
                     /locus_tag="VQ846_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET3075764.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(4369..5640)
                     /locus_tag="VQ846_001186"
     CDS             complement(4369..5640)
                     /locus_tag="VQ846_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET3075765.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            6190..7707
                     /gene="malQ"
                     /locus_tag="VQ846_001187"
     CDS             6190..7707
                     /gene="malQ"
                     /locus_tag="VQ846_001187"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET3075766.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLGASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            7733..9991
                     /gene="glgP"
                     /locus_tag="VQ846_001188"
     CDS             7733..9991
                     /gene="glgP"
                     /locus_tag="VQ846_001188"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HET3075767.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKIDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(11036..11608)
                     /locus_tag="VQ846_001189"
     CDS             complement(11036..11608)
                     /locus_tag="VQ846_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075768.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGNWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(12231..13079)
                     /locus_tag="VQ846_001190"
     CDS             complement(12231..13079)
                     /locus_tag="VQ846_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET3075769.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYNKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            13231..13509
                     /locus_tag="VQ846_001191"
     CDS             13231..13509
                     /locus_tag="VQ846_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075770.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            13511..15574
                     /locus_tag="VQ846_001192"
     CDS             13511..15574
                     /locus_tag="VQ846_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET3075771.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRICVTD
                     NQMNSRLLQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLTRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(15652..16908)
                     /locus_tag="VQ846_001193"
     CDS             complement(15652..16908)
                     /locus_tag="VQ846_001193"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET3075772.1"
                     /translation="MHIFDELKDRGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQELDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            17050..19515
                     /locus_tag="VQ846_001194"
     CDS             17050..19515
                     /locus_tag="VQ846_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET3075773.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            19580..20491
                     /locus_tag="VQ846_001195"
     CDS             19580..20491
                     /locus_tag="VQ846_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET3075774.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            20721..21419
                     /gene="dapD"
                     /locus_tag="VQ846_001196"
     CDS             20721..21419
                     /gene="dapD"
                     /locus_tag="VQ846_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET3075775.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            21487..22617
                     /locus_tag="VQ846_001197"
     CDS             21487..22617
                     /locus_tag="VQ846_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET3075776.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALACELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            22629..23168
                     /locus_tag="VQ846_001198"
     CDS             22629..23168
                     /locus_tag="VQ846_001198"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET3075777.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLMPKTYPKGRMDFVVYDPQQLVKTSFALLEPQ
                     GDVEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            23152..23829
                     /locus_tag="VQ846_001199"
     CDS             23152..23829
                     /locus_tag="VQ846_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET3075778.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGL"
BASE COUNT         6983 a         4390 c         5268 g         7255 t
ORIGIN      
        1 tatcaagttt tttcaatacc tgatagtatg cgtttttgct tttaacgact ttttgtccag
       61 tctcttattt ttcgataatt ttgtgggcga tcaattgacg gtaacaaatt tgtttattgt
      121 ttttattatc attaataatc tggagaatag tttcaaggga aacacgacca agctctaaac
      181 tattgatatc gacataggct gccaagttga gcttgggatt aaccgagtca aagctgagaa
      241 cagggacatc cagctggtgt ttggcaatat agttacaaac accttcagct aggaggctat
      301 cggttgtgat gatagcatca atttgtggat cgtgcttgaa taatcgcttg ctaaatttat
      361 agcccttttc ttccagaaac tcgtcggcaa agtagatgcg attgttgtca gtggtaagtt
      421 tgtaatgttt aagcgcctgt tcatagcctg ttaaacggtc tttggtcacg aagagctttt
      481 tacttcctcc gataaaggca atgcgtttgc agcctttttt gatgaaatat tcagtcgcat
      541 caaaaccagc ttgaacattg tcgttgtcga caagtgggat gaaaggagat agagatttac
      601 ctaagataag gaaggggaac tgttcttctg cgacgagttt tacgagaggg tcttcttctt
      661 gggcatagag aaagattagc ccatctacac gcttgccgta gaccatttgt gaaatagcgt
      721 tgagacgctc cttctcatct ttccctgttg ctatctgaat ggcatagtgg ttttcagatg
      781 cgacttgaga gatgccacgt agaaccgatg gaaagaaagg attctggtag aaggcgtctg
      841 agtcatcagg aagaactaat ccgataacct gagtatagct gcttaccaag ctacgagcgt
      901 tgaggtttgg gtggtagttg agttccttca tagctttgcg aacgcgtttt tttgtttcgt
      961 cgctaatggt tgatttattt tgaataacac gggttacggt tgaaggcgaa acaccagcag
     1021 ccttggccac gtctttaatc gtaacgggca taaaaatctc ctatttatgg taatttggat
     1081 cacggaaatg tgttttataa aagatagtga ccagatacag aacaaaaaga atattttgta
     1141 cagtaatcag ggttgtcata ttttggccaa ataatcccaa aataagtgta atcagagtcg
     1201 gcaatcctaa acagttcaag ataaagtgat agcactcttt aaaggttcta aatgaaaaga
     1261 ggcgtgattt cttagtgata taaaggagaa gactagctcc tagagagacg ataaagaaat
     1321 tcaaaccaaa gaggaagctc gcaccgagaa ctaggaagag gctgatatag acacgatttt
     1381 gttggtacca gtctttagaa attgcttggg tcaagctgtc tttgcttttg aaactctcag
     1441 tctgaatggc tcggtaagag atgcgggtca gttccttgct ttccttgctg atgactagct
     1501 catttgtatc gaaatgcagt tgcaagttct taggtaattc cttgatttga cttggaccaa
     1561 tcacaacaga agaggcttga ctaactgttc cagtataagt taatgtgcca tcgacaattg
     1621 tagcatgttc agagagatcc tggacaacct catttgtcag aggttcatag acattatcga
     1681 taaaagtttc tagcggatag gtctcctggg agctgttttg gatagcaatg ggtaccatag
     1741 acaagctgat aaggaagata ctggtaaaga gaagttgaaa ccagttgagc ccgaaacgtt
     1801 tggacagggg cttacgaaac cccaaaatac ttgaaaaaaa ggaaaatgga tatggaagca
     1861 taggtatctt tctaaaaggt gttttctata taagtattat tatatagaga aatgcgcttt
     1921 atttcaagtc taggagaaaa attgcttgct acaaggatat tatttacagt gacaatcgct
     1981 aatagtcaat gaaaatcaaa gtgcaaacca ggaagctagc tgcaggttgc tcaaagcaca
     2041 actttgaggt tggagataaa gctgacgtgg tttgaagaga ttttcgaaga gtataaaatg
     2101 aaaaagggtg gcggggataa attatccctt gtcgccacca cttgtaagtc ctgaaacaaa
     2161 gttcttttgt aggaagaaga agagaataca gattggaagg gcgatgagga tagcacctgc
     2221 tgagaagtag gcaatcttca agtttttcgc attgttaacg aaggtttgga gacctacggc
     2281 aacagtaaag tattctttct cacgaagcaa gaaactagag aggatgtagt ccccgaaagg
     2341 tcccatgaag gcccagagag cttgtacggc aaccattggg cgaacaagtg gaagaacaat
     2401 ttgccagaag cggcggaagt gtcctgcacc gtctagtttt gcagattcgt ctaaagacat
     2461 tggcactgta tcgaagtagc ctttcatgag ccaagcattc atcgggatac caccaccaac
     2521 gtagaggaag atgaggaacc agttgtggtt aagggcgttc aacataagcg ccataacgaa
     2581 gaaggctgtc aaagcggcca tagttggcac catttggatg atcaagaaga agaccaaact
     2641 ttgtttacga gccaagaagt tgtaacggct gtaagcataa ccagcaagta cgatgatact
     2701 tgtttgaaca gccatggtaa ttaaggcgat aatcaaagtg ttgaggtacc aagtaccgta
     2761 caaggtttca gtgaagaggc ctttaaagtt atcaaaattg aggtcgatat tagtatctag
     2821 tttaaaggct gagacgttac ctgctttaaa ggctgacata atggtaatca acagtggata
     2881 gataattaca attgatagac caatcaggta aaggtaagta aggctttgag tcagtctacg
     2941 tttgagttta attgagttat tcatcttaga cgtcctccat atcaaatgcg tgtagtttct
     3001 tgaatgcgat catagagatt gagatgacaa tgatagagat aatcaaggta acagctgccg
     3061 ccattgagta ttgaggagat gtacctgttg tcaaacggta gatccatgag atcaagatat
     3121 cggttgaacc agctccacct ccgacactac caggtcctcc accattgaag aggtacatga
     3181 tagagaagtt gttaaagttg aaggtgtatt ggctaatcaa agtaggtgcc gcaacagcca
     3241 aaatcattgg gaaagtgatg ttgcggaatt tttgccaagc gttggcaccg tcaatataag
     3301 ctgcttcgta aaggtcgtta ggaatagatt gcaagatacc caaagtcaaa acgtagatgt
     3361 atgggaatcc gagccaacct tgcatcataa tcaaggcaat cttagtccaa gttgggtctg
     3421 ttttccaagg aataagagct ccatcaagga aaggaaggaa tttagccaag attggcaata
     3481 cttgagtgtt gatagcaccg acactatcgt taaacatgtt tgagaatgtc aagatagtga
     3541 tgaaggctgg gacagcccaa ggaagaagga aaataacacc aaagatacgt tttcctttga
     3601 taaatggttg gttggcaatg atagctgtga agataccaat tacgatttgt aaagtagaag
     3661 ctgccaaagc ccaaatgata gtccaagaaa gaacagaacc aaaggcagaa cggaaggtac
     3721 tcaagctcca aatgtttgta aagttggtca aaccaaccca gtccaacaac ttgtttggtg
     3781 gcaagtgttg gaagtcgtag ttggtaaagg cgatcatcaa ggttacgata actgggaaga
     3841 taatcgcgaa ggtcatggca acataagatg gaatgatcaa gaggtaaggg aagccatttt
     3901 cataaatccc tttgatcatg tctttgagtg tgcgtggaac tggaattcca ttgttaatgc
     3961 gttttgcaat cgtatgtgca tctttgatat ttgagaaata aaagagtaca taaacgatta
     4021 caaagattag atggaaggca ccacgaatca gcataaagag ggaattatca cgacctggtt
     4081 tgtcaccaag agtgatgagg ttgctcaatt caggggttgc aagtgctagg aagtaaagga
     4141 caaatacgat ggttacacca aggaagataa aacctttggc tttttgttta ttgtaaatct
     4201 gtcctaaccc aggaatgata gacagcaggg ctgctttact aggttgttgc ttttccatat
     4261 taactccttt cataggatac tactttttta taagaaagct aaattatttg tgtctctatt
     4321 gataaattca aagggggatt tgatttccac cccccttgaa caaatttttt attcaccaaa
     4381 tttttgtttg attgtttctt tgatcaatgt tacagcatcg ttagcagctg ttttagcatc
     4441 tttttgacca cttacagcat caaagagcat atttttcgct ggatcccaaa ctgcagacat
     4501 ttgagagatg tttggcagtg gttgagtgtt cttgaactgt ttgataacag ctgttgtcaa
     4561 ctcatcgttt ttaccttcag cgtatgaacg agcctcagta ttagctggga tttcgttagt
     4621 cttatcatat aatacttttt gttgttcagt tgcaacaagg aagtctacaa atttttgaga
     4681 agcttcaagg ttcttaacgg cttgaggaat gacccaagct ttaccaccac cgaatgcagc
     4741 atattctttt ccatttggaa gagttgggat agttgcaact ccgtagttta ctttagcatc
     4801 tttaaaggct tgagctttcc aaggtccgtc gatgatagca gctgttttac cttcttggaa
     4861 ttgagtttgg attaagtttc cagcaccttc tgtatcttgc atacctttag gccatttttc
     4921 gtaccaagat ttagcgtagt tgatacctgc gatagaaccg tcgtttgcaa gaccgatgtc
     4981 tttagcgtct ttaccgtttt ggccaaagac gtaagcaccg ttaccggcaa gaagtccata
     5041 tgtatagtag aagtttgtcc agtcagctag gaaggcagta gttttaccat cttcaccagc
     5101 gaatgcgtat ttgctatctt tagcaaggtt ttccaagtca gcaaatgttt ttggagcatc
     5161 tttcaccaag tctttgttgt agtacataac aagtgactcg ataacggcag gagcaccgta
     5221 aactttacca ttagcagctg ttacaagaga tttagttgtg tcgtctgttt tagcaccatc
     5281 gctcaatttc acttctgaaa gttgtccgtc agaaccaagg ctacctacac ggtcgtatgg
     5341 agccatcata acatcaggga cattaccaga ttggttgtca agagaaagtt tatcaagacc
     5401 tcctagagca tcaccagttt taagagtgac ttttactcca gcttcttttt cataagcttt
     5461 agcaacctct tcaatatagc tcttatatcc ctcgtctaca tatacagtga gttctttgac
     5521 ttcagatgaa ccagaatcag caggcttatc agcagttttg cttccgcaag ctaccaaaag
     5581 caagctagca agtgtaacag ttccaagcac cgcagtgctc ttcataaatt tagatgacat
     5641 agtgtattcc tcccaaagaa tagcaagttt tattgataag gaaacgcaaa cgttttcctt
     5701 tatgagctta gtatagcaca aatagaaaac ggttgcaagt attttttgta aaaattttaa
     5761 aaaattttta agcttgattt gatagcataa ttttgcattt taatagaaga aaagtatgaa
     5821 atagataaga atacagtatt tttaataaaa atatatggaa tctgtctaaa gagggaaacc
     5881 aggaggggct acctccctgt ttctaatccc tatctatttt ctatagacgt ttgtgctttg
     5941 ttgcatataa attcaagctc cctaaggagg ttttgtactt tgtagactgt ctcgtgagag
     6001 tcaaacttta ggattagata gttttaagtt atctttgcta aaatgagtta gtctccttaa
     6061 aaataatcaa aaaagttttt taaaaacgct tgcaattatg cttgaaaagg agtatactta
     6121 taagtaacgc aaacgtttgc gtctgcaaaa tacgcaacgt tccattattt taacacacga
     6181 ggtgctatta tgaaaaaacg tcaaagtggt gtgttgatgc acatctcttc tcttccagga
     6241 gcttacggaa tcggatcatt tggtcaaagt gcttacgact tcgttgattt cttggtccgt
     6301 acaaaacaac gttactggca aatccttcca ttaggagcaa ctagttacgg ggattctcct
     6361 taccaatctt tctcagcctt cgcaggaaac actcatttta tcgatttaga tatcttggtg
     6421 gagcaaggtt tgttgggagc gagtgacctt gaaggagttg actttggtag cgatgcgtct
     6481 gaagttgact atgctaaaat ctactatgca cgtcgtcctc ttttagaaaa agcggtgaaa
     6541 cgtttctttg aagtcggaga tgttaaagat tttgagaaat ttgctcaaga caaccaatca
     6601 tggcttgagc tctttgctga gtatatggct atcaaagagc attttgacaa tcttgcttgg
     6661 actgaatggc cagatgcaga tgctcgtgct cgtaaagctt cagcacttga aagctatcgt
     6721 gagcaattgg cagacaagtt ggtttaccac cgtgtgactc aatacttctt cttccaacaa
     6781 tggttgaaat tgaaagctta cgctaacgac aaccacattg aaatcgttgg ggacatgcca
     6841 atctacgtag cggaagattc aagtgatatg tgggcaaatc cacatctctt caaaacagat
     6901 gtcaatggta aggctacttg tatcgcagga tgcccaccag atgagttttc tgtaactggt
     6961 cagctttggg gtaacccaat ctatgactgg gaagcaatgg acaaagacgg ctacaaatgg
     7021 tggattgaac gcttgcgtga aagcttcaaa atctacgata tcgttcgtat cgaccacttc
     7081 cgtggcttcg aatcttattg ggaaatccct gctggttccg atacagcagc acctggtgag
     7141 tgggtgaaag gtccaggcta caagcttttt gcagccgtta aggaagaact tggtgagcta
     7201 aacatcatcg cagaagacct tggcttcatg acagatgaag tgatcgaatt gcgtgaacgt
     7261 actggcttcc caggaatgaa gattcttcaa tttgccttca acccagaaga cgaaagcatt
     7321 gatagcccac acttggcacc tgctaactca gttatgtaca caggaacaca cgataacaat
     7381 acggttcttg gttggtaccg taatgagatt gatgatgcga ctcgtgagta catggctcgt
     7441 tacacgaacc gtaaagaata cgaaacagtg gtacacgcta tgcttcgtac agtattttca
     7501 tcagttagct ttatggcaat tgcgactatg caagatttac tagaattgga tgaggcagct
     7561 cgtatgaact tcccatctac ccttggtgga aactggtctt ggcgtatgac tgaagatcaa
     7621 ttgacaccag ctgtcgagga aggtttgctt gacttgacaa caatttatcg ccgaattaat
     7681 gaaaatttgg tagatttaaa gaaataagac aataatcagg agacaactaa acatgttatc
     7741 actacaagaa tttgtacaaa atcgttacaa taaaaccatt gcagaatgta gcaatgaaga
     7801 gctttacctt gctcttctta actacagcaa gcttgcaagc agccaaaaac cagtcaacac
     7861 tggtaagaaa aaagtttact acatctcagc tgagttcttg attggtaaac tcttgtcaaa
     7921 caacttgatt aaccttggtc tttacgacga tgttaaaaaa gaacttgcag ctgcaggtaa
     7981 agacttgatc gaagttgaag aagttgaatt ggaaccatct cttggtaatg gtggtttggg
     8041 acgtttggct gcctgcttta tcgactcaat tgctactctt ggtttgaatg gtgacggtgt
     8101 tggtcttaac taccactttg gtcttttcca acaagttctt aaaaacaacc aacaagaaac
     8161 aattccaaat gcatggttga cagagcaaaa ctggttggtt cgctcaagcc gtagctacca
     8221 agtaccattt gcagacttta ctttgacatc aactctttac gatattgatg ttactggtta
     8281 tgaaacagcg actaaaaacc gcttgcgttt gtttgacttg gattcagttg attcttctat
     8341 tattaaagat ggtatcaact ttgacaagat agatatcgct cgcaacttga ctctcttcct
     8401 ttacccagat gatagtgacc gtcaaggtga attgctccgt atcttccaac aatacttcat
     8461 ggtttcaaac ggtgcgcaat tgatcatcga cgaagcaatc gaaaaaggaa gcaacttgca
     8521 tgaccttgct gactacgcag ttgtccaaat caacgatact cacccatcaa tggtgattcc
     8581 tgaattgatt cgtcttttga ctgcacgtgg tatcgagctt gacgaagcaa tctcaattgt
     8641 tcgtagcatg actgcctaca ctaaccacac aatccttgct gaggcgcttg aaaaatggcc
     8701 tcttgaattc ttgcaagaag tggttcctca cttggtacca atcatcgaag aattggaccg
     8761 tcgtgtgaag gcagagtaca aagatccagc tgttcaaatc atcgatgaga gcggacgtgt
     8821 tcacatggct cacatggata tccactacgg atacagtgtt aacggggttg cagcactcca
     8881 tactgaaatc ttgaaaaatt ctgagttgaa agccttctac gacctttacc cagaaaagtt
     8941 caacaacaaa acaaacggta tcactttccg tcgttggctt atgcatgcta acccaagatt
     9001 gtctcactac ttggatgaga ttcttggaga tggttggcac catgaagcag atgagcttga
     9061 aaaactgttg tcttatgaag acaaagcagc tgtcaaagaa aaattggaaa gcatcaaggc
     9121 tcacaacaaa cgtaaattgg ctcgtcactt gaaagaacac caaggtgtgg aaatcaatcc
     9181 aaattctatc tttgatatcc aaatcaaacg tcttcacgag tacaaacgcc aacaaatgaa
     9241 cgctttgtac gtgatccaca aataccttga catcaaagct ggtaacatcc ctgctcgtcc
     9301 aatcacaatc ttctttggtg gtaaagcagc tccagcctac acaatcgctc aagacattat
     9361 ccatttaatc ctttgcatgt cagaagttat tgctaacgat ccagcagtag ctccacactt
     9421 gcaagtagtt atggttgaaa actacaacgt tactgcagca agtttcctta tcccagcatg
     9481 tgatatttca gaacaaatct cacttgcttc taaagaagct tcaggtactg gtaacatgaa
     9541 attcatgttg aacggagctt tgacacttgg tactatggac ggtgctaacg tggaaatcgc
     9601 tgagttggtt ggagaagaaa acatctacat cttcggtgaa gattcagaaa ctgttatcga
     9661 cctttacgca aaagcagctt acaaatcaag cgaattctac gctcgtgaag ctatcaaacc
     9721 attggttgac ttcatcgtta gtgatgcagt tcttgcagct ggaaacaaag agcgcttgga
     9781 acgtctttac aatgaattga tcaacaaaga ctggttcatg actcttcttg atttggaaga
     9841 ctacatcaaa gtcaaagagc aaatgcttgc tgactacgaa gaccgtgacg catggttgga
     9901 taaagtcatc gttaacattt ctaaagcagg attcttctca tctgaccgta caatcgctca
     9961 gtataatgaa gacatctggc acttgaacta atactcttcg aaaatctctt caaaccacgt
    10021 cagctttatc tgcaacctca aagcggtgct ttgagcaacc tgcggctagc ttcctagttt
    10081 gctctttgat tttcattgag tataagatac aaatttatac taatacatct tgtaaaaaag
    10141 cgagtttcga ttgaaattcg cttttttaaa tgctgtggat ttgggtcaat cttgtctaaa
    10201 aatagggaaa tcctagatac agtgaaggct ttaaatgctg gtttttgctg tcttcagcct
    10261 tatatttttt cgtagttggt tacctcatat ctcttgtatt cgcttacata aagtattata
    10321 atataattgt aggaaagaag gtgtttttat gatatacaca cttaaattgg tgttgtttat
    10381 tacctttctt gtaataagct tgttacctga taagattttt ggaaaaaata aaaaaatttg
    10441 gaaaatagtt tttgcaatat tgacggcagt ggcagcattg tcatttatgt actaagttat
    10501 tttaagaatg tagggaaata aaccctacat tctttttagt tttttctgtt ttctaaattc
    10561 tatttatcca agcgcttcaa catttcttgc ttcttcgctt caagttctgc acgcttttct
    10621 tcgatttcgg catgtttttt ctcgagttca gaacaacttg caccattgct aaattctttt
    10681 cgccatcagg agatagggtg agtcgacatg tctattactc acccaaagca gtcctacaaa
    10741 gcaggaattt tctgttactt ttttggaaat agtaacgttt atacagcttt gacacttcgt
    10801 atcaaagcgc caaacacact ccgagggatt tacagaaagc agaaaaggaa tgatctggta
    10861 taagatcatt ccttttctct ctttttcttt aagtaattat atacaatgta cgacgaagtc
    10921 gtcattgcaa tgctgatcca ccacctaaag ggaactttaa acaacattga taagataaag
    10981 aatataaaca acgaaaatac gttataccca attaatttta ttgtatatct catgattaaa
    11041 agttaatcct tccgttgtta ggaatggcat catttttatc ccataattgt gctaaataag
    11101 tccccggtga taataaattc atagcggatt ctaaagcaac atcatttaca aaccaattac
    11161 ctagatatct agaaattgct gaacgaatag cactttttgc tgcatgtttt ccttttactt
    11221 taattagatt tgcaaggcct gcagtagttc ctcctaatgc taaagctatt gcagtatcta
    11281 atagagcacc catttgatta actgtaatac cttgccaagc tgctctaaat ggagagtatg
    11341 taggtgggat tgtataatcg ccttgtaatt gtcggttaat tacttctttg atccattgtt
    11401 gtgagacgtc tggatgaaaa gattggattt cgtttgcaag tgtattgatt tgttcttctg
    11461 ttagagaagt gacaggttga agttccatat ttgtttcaat ttgtgatact tgttcagaag
    11521 cgtatacagc tgaaacactt ggaatcgctg atacaattaa cacaattgac gtcaaaaaaa
    11581 ccgaaataaa tttcattaat ttgttcatga gcttttctcc tttttatttg tatctgctta
    11641 cattttatta tatactgtta ttatagtcaa taaaaatatg ctattatgtt aaaaaaatat
    11701 ttttaaaaat ataaatggac ggatttattt tggattttat ttgttatttt gacctgcctc
    11761 tatattggta accatgattt gtttactttc aatcatcaag aattctcttt tcgtggtagc
    11821 gtttggggtc tggtactggc cttatatcac ttattgttca ttgataagtt tgtaatttcg
    11881 aatcgaaaat aaagattaga actatgattg actggctact tttaatattg attttggagg
    11941 aagattttgt ctctattatt tattatttta aatttattta ttttgtataa gatctattct
    12001 ttgaggcggg aaaaatcgaa atacttgatt tatacggcct atatcatatt tggggtaaat
    12061 gtagtatatg gtattcaatg gttattaaga gaactgattt caactatttc cccttaatgt
    12121 gtatggaaac tataacaaaa cgcataagat taaggtttgt gtacctgttc ttatgcgttt
    12181 ttataatatt gaagattttc ggtaagtttt atacctttgg gaagtctagt ttagaatgct
    12241 ttcccaacca aaatctctgc ttcgatggta atctctgtca gttggctcca gtcaatcttg
    12301 ctctggtcaa cgtgaaagag gagaggagtc atactgagta gggcttggag ttgttctgct
    12361 gtgatagtct tagtcagaga ggcagtttga ctagatagga tggtaaagtg ttcctggaaa
    12421 tgctccttga tatcttggtt agaatactcc ttgtttgtca gctggtcctg tactctttga
    12481 cggatttctt tgaggtgatt ttcagttggg ataaccttta tcaagatacc gtctttggat
    12541 aaaacgcgac gaaattctcc atagttggca ggtgagaaga tatcaagcag aatatccatg
    12601 ttagcgtctt ttataggaag tcgtgccaag tcgccaacaa accaattgac tgcccagttg
    12661 ggttcacttt tagcagcgat ttggactgaa tctttggaga tgtcaaaggc atagaaagtt
    12721 ttttcagagt gactttcttg tagtttgcga gaatagaatc cttcaccaca accgatatcc
    12781 aaaattgttg tggtagtttt tgagcttgca agcaagtcag atacagcatc taagatagct
    12841 tggtaaaagc cggcttctag gatttgttga cggttttgaa aattttcctt gttatagtta
    12901 gcagattgct tgatttgagg tgctagattg acatagccaa atttcgccaa gtcaaaagaa
    12961 tgacggttgc agcacttgaa attagtctct aacagagtca gattttcttg acagatagga
    13021 caggcaaagg cagtcgcaga agcaaaacgc tgaagtttgg gcttgagatt tgtattcata
    13081 gtttaagtgt atcaaaagag gcaaatgaaa gcaactttag gaataagtag atgggagatt
    13141 cagtagaaat aaaactaatt ctccaattta tagaatgaaa ttgcttttat atctaaattg
    13201 ctatataata atgataagga ggaaataagt atgttaaaag aagtattaac cgttgcaaaa
    13261 gttgcgaaaa aatcttcatt atttttgggt ggtgtcgcat ttggtaccct tggtttgaaa
    13321 atcttagcaa gtaaggaagc taaaaaaggt tattctaaag ctttggctaa ggcttacaag
    13381 ttgaaagacg agctagatgc atctgtttct gttgtgaagc aacatggaga cgatgtcttg
    13441 caagatgcca aatatttgta cgagcaagag aaaaaagaag agcaattaga tagccttata
    13501 ggtgaataat atgtctttta aagtgctaca tagaggatac caacatatcc gactatcatc
    13561 ttctttttca cttaccttgg atattcaaga ctatcttcgt tccttggcga gagatgaaaa
    13621 ggggattgag tctatccagt tttacatgga tcaacagcac tttactctac gcataaaaga
    13681 aggcttttct gtattagata atgcagaagc ctttttaaaa agaattgata aagggaaagt
    13741 ttctgagttg atgactcttc ccattcgtag agaagagagt gcttattcta ttgtttcagg
    13801 tgcagcggtt aagcgtgtac tttttcgtag ttttgtgccg tatcctattc gctatatatg
    13861 gacttgttat caggctttgg gttatattag agaagcctat caaacactag cgcgtaagga
    13921 actaacgatg gaagtcttgg actgttcggc tattttattg tctttgttta tgaaccaatc
    13981 caagacagct agcaatatca tgtttatgct tgatttgggg aatcatttag atcagtggtc
    14041 cttgaaaaaa actgcaacag atttagaaca gagtcttctt gcaaaagaga gcgatgtatt
    14101 cctagtacag ggcgatacgg ttgttagtat caagagttcc gatgttcaaa taggagatgt
    14161 cttgatctta tctcaaggaa atgaaattct gtttgatgga caagtagttt caggtttagg
    14221 tatggtcaac gaaagttcct tgacaggaga gagttttcca gttgaaaaaa gagagtctga
    14281 tttggtttgt gcaaatacag tattagaaac tggagagtta cgcatttgtg taacagataa
    14341 tcagatgaac agccgtcttt tacagctgat tgagttgatg aagaaatctg aagaaaacaa
    14401 gaaaacgaaa caacgctatt tcatcaagat ggcggataaa gtcgtcaaat ataatttctt
    14461 gggggctggg ctgacttacc tattgacagg ttctttttct aaggctattt ctttcctatt
    14521 ggtcgatttc tcctgcgctt tgaaaatctc tactcctgta gcttatttga cagttatcaa
    14581 ggaagggttg aaccgtgaaa tggtgattaa ggatggagat gttctggaga aatatctgga
    14641 agttgatact ttcttgtttg ataagacagg aacaatcaca actagttatc ctatagttga
    14701 aaaggtgtta ccttttggag actatagtga ggaagatatt ctcagaatca gtgcctgtct
    14761 tgaggaacac atttatcatc ctattgctaa tgccatcgtc aagcaagctg agatagaggg
    14821 aattgaacat gaggaaatgc atgggaaact ccaatatatc gcaagcaagg ggatcaaatc
    14881 tcatatagat ggccaaccag ttcttattgg gaattatgtc ttgatgcagg atgagcagat
    14941 tcatatcagt tcagaacaaa atgctttaat tgaagagtac aagagtcact acaatctctt
    15001 attcttggct tatcaaaatg aattgattgg aatgttctgc attcatactc ctttgagaaa
    15061 agaagcaaaa acagccttgg ataaacttaa ggcacagggg aaaaaattga ttctggcaac
    15121 aggagacacc ttgactagga cagaggaatt agtcaaagat ttgccctttg atcaggtcta
    15181 tacagacttg aaacctgatg ggaaatttga gttagtagag aaactgcaga aagcaggtca
    15241 cactattttg atggttggag atggattgaa tgactcagcg gctttaactc tatcagatat
    15301 cggtgtggtg atgaatgaga gtgcagatat ttctaagcag atgagtgata tcttattgtt
    15361 agataatcgt ttggatttct tccaagagtt ggattcgtta tcatcatctt tgcaaacact
    15421 catcaagaaa aatattcaag ataccgttgt cgtaaatagt agtttgattg gcttcggctt
    15481 atttaattgg ctcagtcctt caaatctctc tatcttacat aatctaacaa ccttgcgcat
    15541 tgtcctgcgt agcctgtcta ttaaaaatag atagatgaga gttattaaca gcaaaaagga
    15601 gttacctttc tcgaggctaa ctcctttgtt tatagataaa tgttgaatag tttagtaagt
    15661 caatacaaag tattttttct tcccacgacg gataacagtc agttcattct ctaacttatc
    15721 agcgtcactc aagacatagt caagctcttg gatgcggtcg ccgtttacgt agatggctcc
    15781 gttttggacg tcttcacggg cttggcgttt tgagttaact ataccagatg agacgagcag
    15841 ttccacgata ttgttgtttt cgtctgcctg tacttggtag tttggcacac cacgaagtcc
    15901 ttgtttgagc tctttgacag aaaggttttt gatgtttcct gcaaagagtt gctcagtgat
    15961 gttaagtgct tctttgtagg cttcttctcc gtgaacaagt gtaacaactt cacgagccaa
    16021 gactttttga gccaagcgtt cgtgtggcgc tgcttcaaat tgtttacgaa tatcttcaat
    16081 ctcatcaagt gacaagaaag taaagatttt caagaagcga acagcgtcag cgtccatcac
    16141 gttcatccag aattggtaca tttcgtatgg agaagtcttt tcgggattga gccagacggc
    16201 atttccttct gatttaccaa atttcttacc agttgcatct gtgattagtg gaacagtgat
    16261 aacgtgacca gtcttgtccg ctttacgacg aagcaattcg gtaccagctg tcatatttcc
    16321 ccactggtca gaaccaccga tttgaagagt gacattatgg tcttggttaa ggacgaagaa
    16381 gtcgtaccct tgcatgattt ggtaagcgaa ctcagtgtaa gaaattcctg tttcgattcg
    16441 tttttttaca gattccttac tcatcatgta gttgaccgtg aagtattttc caatatcacg
    16501 gaggaagtca atgaagctga tgctgccaaa ccagtcgtag ttgttgacca tgacagcctt
    16561 gttttcgcca ttttcaaagt caagaaaacg agaaagttgt ccttggatag acttgaccca
    16621 gccatctact gtgtcttttg tttggagact acgttcagca tctttgaagg acggatctcc
    16681 gatgagacct gtagcaccgc caacgagcgc ataaggtttg tgacctgcta gttgcaagcg
    16741 acgacttgtc aagattgcga caaggtggcc taggtgaagg ctgtcagcag ttggatcgta
    16801 gccagtataa taagaaactt gaccttcttc tagggcttta cgcaaagctt cttcatcagt
    16861 cgtttgaaat atcaaaccac gatcttttag ctcatcaaaa atgtgcatgt gtcttttctc
    16921 ctttttaaaa ttattgtttc tacctattgt atcacaaact tgggcaatag cctagcagaa
    16981 tagggaagaa agtggctatt ttattgaaaa tttccctttt gtggtataat agatagagtg
    17041 aggaaatcca tgcaaaatca attaaatgaa ttaaaacgaa aaatgctgga atttttccag
    17101 caaaaacaaa aaaataaaaa atcagctaga cctggcaaga aaggttcaag taccaaaaaa
    17161 tctaaaacct tagataagtc agccattttc ccagctattt tactgagtat aaaagcctta
    17221 tttaacttac tctttgtact cggttttcta ggaggaatgt tgggagctgg gattgctttg
    17281 gggtacggag tggccttatt tgacaaggtt cgggtgcctc agacagaaga attggtgaat
    17341 caggtcaagg acatctcttc tatttcagag attacctatt cggacgggac ggtgattgct
    17401 tccatagaga gtgatttgtt gcgcacttct atctcatctg agcaaatttc ggaaaatctg
    17461 aagaaggcta tcattgcgac agaagatgaa cactttaaag aacataaggg tgtagtaccc
    17521 aaggcggtga ttcgtgcgac cttggggaaa tttgtaggtt tgggttcctc tagtgggggt
    17581 tcaaccttga cccagcaact aattaaacag caggtggttg gggatgcgcc gaccttggct
    17641 cgtaaggcgg cagagattgt ggatgctctt gccttggaac gcgccatgaa taaagatgag
    17701 attttaacga cctatctcaa tgtggctccc tttggccgaa ataataaggg acagaatatt
    17761 gcaggggctc ggcaagcagc tgagggaatt ttcggtgtag atgccagtca gttgactgtt
    17821 cctcaagcag catttttagc aggacttcca cagagtccca ttacttactc tccttatgaa
    17881 aatactgggg agttgaagag tgatgaagac ctagaaattg gcttaagacg ggctaaggca
    17941 gttctttaca gtatgtatcg tacaggtgca ttaagcaaag acgagtattc tcagtacaag
    18001 gattatgacc ttaaacagga ctttttacca tcgggcacgg ttacaggaat ttcacgagac
    18061 tatttatact ttacaacttt ggcagaagct caagaacgta tgtatgacta tctagctcag
    18121 agagacaatg tctccgctaa ggagttgaaa aatgaggcaa ctcagaagtt ttatcgagat
    18181 ttggcagcca aggaaattga aaatggtggt tataagatta ctactaccat agatcagaaa
    18241 attcattctg ccatgcaaag tgcggttgct gattatggct atcttttaga cgatggaaca
    18301 ggtcgtgtag aagtagggaa tgtcttgatg gacaaccaaa caggtgctat tctaggcttt
    18361 gtaggtggtc gtaattatca agaaaatcaa aataatcatg cctttgatac caaacgttcg
    18421 ccagcttcta ctaccaagcc cttgctggcc tacggtattg ctattgacca gggcttgatg
    18481 ggaagtgaaa cgattctatc taactatcca acaaactttg ctaatggcaa tccgattatg
    18541 tatgctaata gcaagggaac aggaatgatg accttgggag aagctctgaa ctattcatgg
    18601 aatatccctg cttactggac ctatcgtatg ctccgtgaaa agggtgttga tgtcaagggt
    18661 tatatggaaa agatgggtta cgagattcct gagtacggta ttgagagctt gccaatgggt
    18721 ggtggtattg aagtcacagt tgcccagcat accaatggct atcagacctt agctaataat
    18781 ggagtttatc atcagaagca tgtgatttca aagattgaag cagcagatgg tagagtggtg
    18841 tatgagtatc aggataaacc ggttcaagtc tattcaaaag ctactgcgac gattatgcag
    18901 ggattgctac gagaagttct atcctctcgt gtgacaacaa ccttcaagtc taacctgact
    18961 tctttaaatc ctactctggc taatgcagat tggattggga agactggtac aaccaaccaa
    19021 gacgaaaata tgtggctcat gctttcgaca cctagattaa ccctaggtgg ctggattggg
    19081 catgatgata atcattcatt gtcacgtaga gcaggttatt ctaataactc taattacatg
    19141 gctcatctag taaatgcgat tcagcaagct tccccaagca tttgggggaa cgagcgcttt
    19201 gctttagatc ctagtgtagt gaaatcggaa gtcttgaaat caacaggtca aaaaccaggg
    19261 aaggtttctg ttgaaggaaa agaagtagag gtcacaggtt cgactgttac cagctattgg
    19321 gctaataagt caggagcgcc agcgacaagt tatcgctttg ctattggcgg aagtgatgcg
    19381 gattatcaga atgcttggtc tagtattgtg gggagtctac caactccatc cagctccagc
    19441 agttcaagta gtagttctag cgatagcagt aactcaagta ctacacgacc ttcttcttca
    19501 agggcgagac gataattctc taaatgaagt ggccaatcaa tggattgcca cttttttctt
    19561 ttcgtgttac aataagggta tgaatcagta tcagaaaaag attgttaacg gaaaaattta
    19621 ttcgctccta tccggcttaa tatggggaat ctgtggaatt ttaggagagt acttctttac
    19681 tcattatcag gtgtcttcgg gctggattac ctctatgcgt ttgacactgg cagggagtct
    19741 tgtactcatt tggtctgcaa tacaattaaa atcgcaagtg ctagatattt ggcgagacaa
    19801 gaaaaattac ctgccctttt tagcctatgc tattttgggg attttttcag ttcagtattt
    19861 tttctatctc tgtgtagaat actcaaatgc tacgacagca actattttac agtttattag
    19921 ccctgtcttt atcctctttt acaatcgctt ggtttatcaa aaacgagcgt caaaaagcgc
    19981 tgttttctat gttttggttg ccatgctggg tgtttgcttg atggcgacaa agggagatct
    20041 ctctcagtta tccatgacgc cgctagctct tataacaggt ttgctgagtg ccatgggtgt
    20101 tatgtttaat gttatcttgc cccaaccttt tgctaagcgt tatggttttg ttcctacggt
    20161 tgggtggggg atgattttgg caggtttgtt tagcaatgtc ctctcgccgg tttatcagct
    20221 ttcctttact cttgatattt ggagtatctt gatttgcctc attatcgctt tctttggaac
    20281 ggcttttgct tttttcattt ccatgaaggc tgtgtccttg gtttctcctt tggtggttgc
    20341 cgttatcagt gccagtgaac ctctctcttc tgctctcttg agtgttttgt tcttaggatt
    20401 agtagtggat tggtccctcc ttctagctat agccttgatt attttaccca tgatattttt
    20461 gtctatagaa gaagcgaaag aaagtagata aaaagtcttt tcttaattct aaaagtgtgc
    20521 tatactagaa tagtcaataa aacacgggga gatagttgtg aagaatgttt ttaggttgta
    20581 tcggtagggg cttgctttta ccgacagcac gttttatctc acaaccctaa aaaatcatac
    20641 ctaaaaacag acaaaaaggt ttcaaatttg aggccttttt tggtagaata ggtatcatta
    20701 taacgaacca ggaggcacct atgactgcta caaaaatgaa cgctcaagaa attatccaat
    20761 ttatcgccaa tgctgaaaag aaaaccagtg tcaaagtaac ctttgagggg caactcgcaa
    20821 ctgctgtgcc tagctctgtt gtcaaactag gaaatgtcct attcggagac tggaaggacg
    20881 tggctccgct tcttgaaggt ttggtagaaa atcaagatta tgttgtcgag caagatgctc
    20941 gtaattctgc agttcctttg ctagataagc gtgctatcaa cgctcgtatc gagccaggtg
    21001 ctattatccg tgaccaggtg gaaattggtg acaatgctgt tatcatgatg ggagctgtta
    21061 tcaatatcgg tgctgaaatc ggtgctggaa ccatgattga catgggtgcc atccttggtg
    21121 gccgtgccat cgttggaaaa aatagccacg ttggtgcagg tgcagttttg gcaggtgtga
    21181 ttgagccagc tagtgctgaa ccagtccgtg tcggagacaa tgttcttatc ggtgctaatg
    21241 cagtggttat cgaaggagtc caaatcggta gtggttcagt tgtcgcagca ggagctattg
    21301 ttacccaaga tgtcccagaa aacgtggtag tagcaggtgt tccagctcgt attatcaaag
    21361 aaattgatgc ccaaactcaa caaaaaacag cgctagagga tgcgcttcgt accttgtaat
    21421 tgtaaaagta aaaaagaggc ggaacccttt ttccagcctc tttctgctat atcggaggac
    21481 agatagatgt tagatttgat tcagactaga cgagatttac accagatacc agagattggc
    21541 ttggaggagt tcaagactca ggcttatttg ctggatgtga ttgagaaatt gactacgggc
    21601 aaggattttg ttcaaattcg tacttggcgg acagggattt tggtctacct gcagggaagt
    21661 cagccggagc gaaccattgg ttggcggaca gatattgatg gcctgcctat cgtcgaacaa
    21721 acaggtctac cttttgcttc tcaacaccaa ggtcgcatgc atgcctgtgg ccatgatttt
    21781 catatgacca ttgccttagg ctgtcttgag cgcgcccttg aggagcaacc taaaaataat
    21841 ctgctctttc tatttcagcc tgctgaagaa aatgaagctg gtggtatgct catgtatgag
    21901 gctggtgctt ttggagattg gttgccaaac caattctatg gtctccatgt tcgtccagat
    21961 ttgaaagtcg gccagattgc gactaatact catacactct ttgcaggaac ttgcgaggtg
    22021 aagatccgtt tcaaaggcaa aggtggccac gcagcttttc ctcatgaagc caatgacgcc
    22081 ttggtggcgg ctagttactt tgtgacccaa gtgcagtcag ttgttagccg caatgtcaac
    22141 ccaatcgagg gagcagtggt gaccttcggc gtttttcaag ctggaaccac caacaatgtc
    22201 atcacagaca cagctttttt acatggaact attcgtgcct tgacacagga catgagtctc
    22261 ttggtgcaaa agagggtaaa aacagttgca gaaggggttg cagctgcctt tgatatggaa
    22321 gtcgaagtgg aactcaaaca aggtggatac ctacctgttg agaacaatcc agccttggcg
    22381 tgtgaactga tggacttctt tgacgaaaaa gacggaatcg agttgattga tatcgaacct
    22441 gctatgacag gcgaggactt tggttatctc ctttcaaagg ttgatggcgt gatgttttgg
    22501 ctaggtatcg atagtcccta cgctcttcat caccctcaaa tgagtcctaa ggaagaagtc
    22561 ttagctattg gggtggctgc ggtgtctagt ttcctgaaaa agaaggcagc agaatagagg
    22621 gcttgtctat gaaatcggaa ttacgcaagc aagtcttgca tgaaatgaag gctatatctc
    22681 aagagcaaaa acaggctata gaccaagctt taaccgagcg gcttttacac catcccttct
    22741 accaagaagc caaggtcatc gcaacctacc tctctttttc tcatgagttt caaacgcggg
    22801 aactgattga gcaggcgctg aaggacggca agaaggtttt aatgcccaaa acttatccca
    22861 aggggcgcat ggactttgtg gtctatgatc cgcaacagtt ggtaaaaact tcctttgcct
    22921 tactggagcc acagggagat gtggaagtgg tggatgcatc tcagattgat ttgattcatg
    22981 ttcctggtct ggcttttacg acgaaaggat atcggatagg atacggtgga ggctattatg
    23041 accgctatct ggaacatttt tctggtcata ctttgagtac ggttcatcct tgtcaaattc
    23101 aggactttat acctgaaaac catgatattc ctgttcagga ggtattaatt gatgaaggaa
    23161 atctttgata gacgttaccc tgtgacgagt ttcttcctct tagtgacggc cttggtattt
    23221 ttactaatgt tggtcactgc aggcggaaac tttgacaggg cagatacatt atttcgattt
    23281 ggagccatgt atgggccagc tattcgcctc tttcccgagc aggtttggcg tctcttgtct
    23341 gccatttttg ttcatattgg gtgggaacat ttcattgtta atatgctttc actttattat
    23401 cttggaaggc aggtagagga gattttcggc tctaagcagt tcttttttct ctatctctta
    23461 tcaggaatga tgggcaatct ctttgttttt gtatttagtc ctaaatcctt agcagcaggc
    23521 gcctctacct ctctttatgg gctatttgcc gcgattattg ttcttcgcta tgcaactcgc
    23581 aatccttata tccaacagct agggcaatcc tatctgacac tttttgtggt taacattatt
    23641 ggaagtgttc tgattccagg aatcagccta gcaggccata tcggtggtgc agttggtggc
    23701 gcatttctag cagttatctt tccagttaga ggagaaaaac ggatgtataa caccagccag
    23761 agattaggag cggtagtctt gttcgtagga ctcgccattt tgcttttcta caagggaatg
    23821 ggattgtgac gactaatact caatgaaaat caaagagcaa actaggaagc tagccgcagg
    23881 ttgctcaaag cactgc
//
LOCUS       DAVBNO010000027        23842 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVBNO010000027 DAVBNO010000000
VERSION     DAVBNO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23842
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.027"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(98..2356)
                     /locus_tag="VQ846_001200"
     CDS             complement(98..2356)
                     /locus_tag="VQ846_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET3075779.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2585..2815)
                     /locus_tag="VQ846_001201"
     CDS             complement(2585..2815)
                     /locus_tag="VQ846_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET3075780.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3005..3688
                     /locus_tag="VQ846_001202"
     CDS             3005..3688
                     /locus_tag="VQ846_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET3075781.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3688..4422
                     /locus_tag="VQ846_001203"
     CDS             3688..4422
                     /locus_tag="VQ846_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET3075782.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVIENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4576..5433
                     /locus_tag="VQ846_001204"
     CDS             4576..5433
                     /locus_tag="VQ846_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET3075783.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5575..6447
                     /locus_tag="VQ846_001205"
     CDS             5575..6447
                     /locus_tag="VQ846_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET3075784.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQDRVSKDKLV"
     gene            6729..7412
                     /gene="rpiA"
                     /locus_tag="VQ846_001206"
     CDS             6729..7412
                     /gene="rpiA"
                     /locus_tag="VQ846_001206"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET3075785.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7426..8637
                     /locus_tag="VQ846_001207"
     CDS             7426..8637
                     /locus_tag="VQ846_001207"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET3075786.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8639..9181
                     /locus_tag="VQ846_001208"
                     /pseudo
     CDS             8639..9181
                     /locus_tag="VQ846_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            9197..10006
                     /locus_tag="VQ846_001209"
     CDS             9197..10006
                     /locus_tag="VQ846_001209"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET3075787.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10482..11213
                     /locus_tag="VQ846_001210"
     CDS             10482..11213
                     /locus_tag="VQ846_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075788.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGCYLAQIAEVEAYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWNAKNGFWDVGLESRKLAVSGKIKHYVVDNDNVVTP
                     LIHNDRDIVTFTGNSRFKHRSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRF
                     FKKQALPRSSSQPSAEPMENIALGK"
     gene            11259..11462
                     /locus_tag="VQ846_001211"
     CDS             11259..11462
                     /locus_tag="VQ846_001211"
                     /inference="COORDINATES: protein motif:HMM:NF012956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET3075789.1"
                     /translation="MDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIRQINIYNRT
                     DTAQDRLANFDVILLDSSGKEIE"
     gene            11487..11999
                     /locus_tag="VQ846_001212"
     CDS             11487..11999
                     /locus_tag="VQ846_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET3075790.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNGETAI
                     QVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12657..13367
                     /gene="deoD"
                     /locus_tag="VQ846_001213"
     CDS             12657..13367
                     /gene="deoD"
                     /locus_tag="VQ846_001213"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET3075791.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13390..13914)
                     /locus_tag="VQ846_001214"
     CDS             complement(13390..13914)
                     /locus_tag="VQ846_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET3075792.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(13967..14203)
                     /locus_tag="VQ846_001215"
     CDS             complement(13967..14203)
                     /locus_tag="VQ846_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET3075793.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(14271..15191)
                     /locus_tag="VQ846_001216"
     CDS             complement(14271..15191)
                     /locus_tag="VQ846_001216"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET3075794.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15389..15793
                     /locus_tag="VQ846_001217"
     CDS             15389..15793
                     /locus_tag="VQ846_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075795.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGICFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            16009..16590
                     /locus_tag="VQ846_001218"
     CDS             16009..16590
                     /locus_tag="VQ846_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET3075796.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16587..17864
                     /locus_tag="VQ846_001219"
     CDS             16587..17864
                     /locus_tag="VQ846_001219"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET3075797.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            17882..18544
                     /gene="deoC"
                     /locus_tag="VQ846_001220"
     CDS             17882..18544
                     /gene="deoC"
                     /locus_tag="VQ846_001220"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET3075798.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18531..18920
                     /locus_tag="VQ846_001221"
     CDS             18531..18920
                     /locus_tag="VQ846_001221"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET3075799.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19011..20063
                     /locus_tag="VQ846_001222"
     CDS             19011..20063
                     /locus_tag="VQ846_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET3075800.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20207..21742
                     /locus_tag="VQ846_001223"
     CDS             20207..21742
                     /locus_tag="VQ846_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075801.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            21735..22793
                     /locus_tag="VQ846_001224"
     CDS             21735..22793
                     /locus_tag="VQ846_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3075802.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            22796..23752
                     /locus_tag="VQ846_001225"
     CDS             22796..23752
                     /locus_tag="VQ846_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3075803.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         6918 a         4300 c         5437 g         7187 t
ORIGIN      
        1 atcataatgg acaaagggca agctgtcaag aaattacagt tattttagta aaaaaagaag
       61 cagaaaaatt tcattttcta ctccttttct ataggattta tttttctttt tcagaacttt
      121 taacttcagc ttttttggca gatttaatct gaatgtttcc cttgctagtc ataactgctt
      181 tgagatcttt ttcgcttgga ttttcaaggt agtagtcagt gattgtgtcc tcaatatagt
      241 cttgaatagt ccgacgaagt gggcgtgctc ccatttttgg atcataacct aggtcaacca
      301 acttttcctt gaccttgtca gttacatcca aacgaatgtt gttgctagag aggcgcttgt
      361 taacatctgc tagcatgagc tcgacaatct gaaggaggtt atccttgctg agagccttaa
      421 attcgataat gccatcaaaa cggttcataa actctgggct aaagaagtta ccgagttcac
      481 cgaggacaga attggtacgt ccttctctag cagcaccaaa tccaacgcta gcttcggtct
      541 ttcctgtacc tgcatttgag gtcatgataa tgatggcatc cttgaagcta acggtgcgtc
      601 cttgcccgtc tgtcaaacga ccatcgtcca agacttgaag aaacatgtgc ataacatctg
      661 ggtgagcttt ttccacttca tcgagaagga tgagagaata tggattgtgg cgaacttttt
      721 cagttaattg accagcctca tcatagccaa cataacctgg aggagcgccg accaacttag
      781 ccacactatg tttttccatg tattcactca tatcaaagcg aatcatacta tcagcagaac
      841 caaaaagttc gatagccagt tgtttggaaa gttctgtctt accgacacca gttggcccaa
      901 cgaagaggaa gcttccgatt gggcggttag gggtaccaag tccgacacga ttacggcgaa
      961 tagccttggc aatcttatcg actgcatcat cttggccaat aacatgagac ttgagatctt
     1021 cggctagatg gatgagttga gattgttctt tctctttcaa atcaccaaca gggatattgg
     1081 ttttctgctc gataatgtgc tcaatagttt tctcgctgat gataggagta tcctggtctg
     1141 tgatcttttt cttttgcatt tccttatact tggcaatctg gtcgcggaag taggccgcct
     1201 tctcaaaatc ttcttctcgt gtagcttgag acttgagatt ttcagcctca atcaagcgct
     1261 gatcaattac tttaggatcc acaaaattca aggtcaagtt catcttagaa ccagcttcat
     1321 ctaggaggtc aatggccttg tcaggcaaga agcgatcttg gatgtagcga ttggaaagag
     1381 ttgcagctgc ttcaatcgca gcatcggtat attgaacgtg gtggtaatct tcgtatttct
     1441 tttgaatccc tttgagaata gtgattgttt catccaccgt tggttcatcg actttaacag
     1501 gctgcatacg acgctcgagg gcagcatcct tttcaatgat acggtattca ttgagggtag
     1561 tagcaccgac tagttgcagt tctccacgag caagggctgg cttgaggata tttcctgcgt
     1621 ccatattacc atcactcgca gaaccagcac caacaatttc atggatttca tcgataaaga
     1681 ggatgatgtc ttcacgtttg cgaatttctt ccatgagttt ttgcatgcgt tcttcaaatt
     1741 gtcctcgaat ccccgttcct tgaactaagc taaccacatc cagacggatg acttgtttac
     1801 cttggagttt atgtggcaca tcgccatcga caattttctg agctagacct tcgacaacgg
     1861 ccgtttttcc gacaccaggt tcaccgataa ggacaggatt attcttcgtt ctacgattga
     1921 gaatctcgat gacacggata atctcatcgt cgcgcccaat aacggggtca atgtctccac
     1981 gacgggcaat ttcagttaca ttaataccaa attcttccag caggcctttt tcttggctag
     2041 gtggcggagt ttgagcagat ccacgatttt gggaaccata accgccgttt ccaccgtaac
     2101 ctccacctga ttgggttggg ggaataggag gagtattgct agaaggtctg aaattgttta
     2161 gatcattgaa gaaatcacca aagggatcga agtcacgatt gttcagatcc gtcatacctt
     2221 tgaagaggct attgttagga tctgttttga taatcttata gcagttttga cagaggtcaa
     2281 tttgtttttg ttttccattg agattggtgt aaagatgaat tgttgagtcg ttaattttac
     2341 agttttgaca aagcatacat ctacctcatt tctttcttta gcctgacttg tttaaaggtc
     2401 aaaaatagtc aaattctata ctctcattat aacaggattg gggtgtaaag caagtaaaaa
     2461 gattgaagct ttgagaagtt gttacaatga aaatttttgt gaatttggac tataaaaaaa
     2521 tcctatttgt gagacaaaaa ggattttact tagacatgca gggacaatcc cagcttgttt
     2581 tagattagta gagaagttcg taaatttcca ttgcgattaa gtcaatgcta tcaaacgtat
     2641 acgtttcgcg atcttctacg tcacgaaggg taaagattgg gccgttttct tcgtcgtggc
     2701 tgtacgcaac ttctgcaaca acaactcctt cgcgttcaaa attacgtttt aaatttccgc
     2761 catcgtttgc cattgcttca aggcggttga tgattctaac caaatgtgat tccattttga
     2821 ttctccttca tttcttatcg ttactatttt accataaagg aggccaactt gactagaaaa
     2881 aactaagatt tctcgctaat tctgctagtt taaagatttt taaataaatt ggataaaaaa
     2941 tttaaatttt aattcaaaac atgttataat catacagtat tctattttag aaagcagtgt
     3001 gactatgaat ttttcttttt tacctaagta tttaccttat tttaactatg gggctgttgt
     3061 gacgattctt atttctatct gtgttatctt tttgggaact attttgggtg ttgtcttggc
     3121 ttttgggcaa cgttcaaagt ttaaaccgct tgtttggttg gccaacttgt acgtttggat
     3181 tttccgtggg acaccgatga tggttcaaat tatgattgcc tttgctctta tgcatatcaa
     3241 tgctccgact attcagattg gaattttagg tgttgatttt tcgcgtctga ttccagggat
     3301 tttgattatc tctatgaata gtggtgctta tgtttcggag actgttcgtg ccggaatcaa
     3361 tgcggttcca aaaggtcagc tagaggcggc ttattcgcta gggattcgtc ctaaaaatgc
     3421 gatgcgttat gtgattttgc cacaagcaat caaaaatatc ttgccagcat tggggaacga
     3481 atttatcacc attatcaagg acagctccct cttatcagct attggggtca tggagttgtg
     3541 gaatggggct acaacagttt ctacaacaac ctatctacct ttaacaccgc ttttatttgc
     3601 agcattttat tacttgatta tgacctctat tctgacagta gccttgaaag cttttgaaaa
     3661 acatatggga caaggagata agaaataatg acagaaacct tgataaaaat tgaaaattta
     3721 cataaatctt ttggaaagaa tgaagtattg aagggaatca acctcgagat taaaagagga
     3781 gaagttgtcg ttatcatcgg tccttcaggg agtgggaaat ctaccttgct tcgctctatg
     3841 aatttgttgg aggaagcaac caaggggaag gttatctttg agggagtcga tattacggac
     3901 aagaagaatg acttgtttgc catgcgtgag aagatgggca tggttttcca acaatttaac
     3961 ctctttccta atatgactgt gattgaaaat attaccttgt cacctatcaa gaccaaaggt
     4021 gaaagcaggg aagttgctga gaagagagcc caagagcttt tggaaaaagt tggtttgcca
     4081 gataaggcag acgcttatcc acagagtctg tcgggtgggc agcaacaacg gattgccatc
     4141 gcgcgtgggt tggctatgga accagatgtt ttgctctttg acgaaccaac ttcagccttg
     4201 gatcctgaaa tggtaggtga ggtattggct gttatgcaag accttgccaa gtcagggatg
     4261 actatggtta tcgtaacaca tgagatggga tttgcccgtg aggtggcaga tcgtgttatc
     4321 tttatggcag acggtgtggt tgttgaagat gggacacctg agcagatttt tgaacaaacc
     4381 caagaacaac ggactaaaga tttcttgagt aaggttttat aatctatttt aaaatttcaa
     4441 gacaagatta gtgaaaagtt caaccagagc tttttcttat agtttgaaac tataggataa
     4501 cctaggaaag aagtgttaga gggcactagc agtttttggt ataatggaag atatttgaaa
     4561 gaaaagagaa gtgatatgac acagattatt gatgggaaag ctttagcggc caaattgcag
     4621 gggcagttgg ctgaaaagac tgcaaaatta aaggaagaaa cagctctagt gcctggtttg
     4681 gtagtgattt tggttgggga taatccagcc agccaagtct acgttcgcaa caaggagagg
     4741 tcagctcttg cggctggttc ccgtagcgaa gtagtgcgag ttccagagac cattactcaa
     4801 gaggaattgt tagacctgat tgctaaatat aatcaggatc cagcttggca tgggattttg
     4861 gtccagttgc cattaccaaa acatatcgat gaagaggcgg ttttattagc cattgaccca
     4921 gaaaaggatg tggatggttt ccatccccta aacatgggac gtctttggtc tggacatcca
     4981 gtcatgattc cctcgacacc tgcaggaatt atggaaatgt ttcatgaata tgggattgac
     5041 ttggaaggta aaaatgcggt cgtaatcggt cgttcaaata tcgttggaaa acctatggct
     5101 cagcttcttt tggctaaaaa tgcgacagta accttggccc actcacgaac tcataatctt
     5161 gccaaggtgg ctgctaaagc agatattctt gtagtcgcaa tcggccgcgc caagtttgtg
     5221 actgctgact ttgtcaaacc aggtgcggtt gtcattgacg ttgggatgaa ccgagatgaa
     5281 aatgggaagc tctgtggaga tgttgattat gaggcagttg caccacttgc tagccatatc
     5341 acgccagtcc ctggaggtgt cggtcctatg accattacca tgctgatgga gcaaacttat
     5401 caagcagcac ttcggacatt ggatagaaaa taagagaaac attcgattaa tagagtgtat
     5461 tttctatagc tatatctaaa atggcagaaa tgaatattaa attttagata taagattaca
     5521 aaaggaggtc tgcgtctcct ttttgttgta taataaaagt gagagggaaa aaggatgaaa
     5581 gtgattaatc aaaccttact agaaaaagtt attattgaac gttctcgttc aagtcataaa
     5641 ggagattatg gtcgtctgct gctgcttggt ggtacttatc cttatggagg tgccatcatc
     5701 atggctgctt tagcagctgt aaaaagcggt gcaggattgg tgaccgttgg aacggacagg
     5761 gaaaatatcc cggctctgca cagccattta cctgagccta tggccttttc tcttcaagac
     5821 cagcaattgt taaaagagca attggagaag gcagaagtta tcttgctggg gcctggttta
     5881 cgagacgatg cttctggaga aaatctagta aaacaggtct ttgttaattt aagccaaaat
     5941 cagattttga ttgtagacgg aggtgccttg accattcttg ctaggacaag tttgtcattt
     6001 ccctcaagcc agcttatcct aactcctcac caaaaagaat gggaaaaatt atctggaatt
     6061 acgattgaaa agcaaaagga agatgcaacg gctagtgttc tgacttcctt ccctcaagga
     6121 acaattttgg tggagaaagg cccagctact cgtatttggg aagttggcca atctgattat
     6181 taccagttac aggttggtgg tccttatcag gcgactgggg gaatgggaga tactctggct
     6241 gggatgatcg caggatttgt aggccaattc cgacaggcca gtctctacga gcgtgtggca
     6301 gtagcgaccc atcttcattc agccatagcc caagaactat ctcaagaaaa ttatgtggtc
     6361 ttaccgacgg aaatcagcag gtatcttccc aaaataatga aaattatttg tcaacaagat
     6421 agggtaagca aagataaact tgtttaaaat agacagataa atctacttcc ttctcttaat
     6481 ttatgattct tgaaatgcgc ctgctttaag gctaaactag acgtatcaaa gttaagagtt
     6541 tgtttttgta actggaatga gatgaacaaa tcaaatacat aaaagataaa ctcattttta
     6601 ttcgtttggt aaaaataaaa tgcatatttt taaagaaaga ttgatgaaaa atgtagattt
     6661 gatgttttta cttgaataaa aagcggtttc atagtaaaat aagaactgag aatgatggag
     6721 gtaaatcggt ggaaaatctg aagaaaatgg caggtatcaa ggctgctgag ttcgtgagcg
     6781 atggaatggt cgttggactt ggaacaggct cgactgccta ttattttgtc gaagaaatcg
     6841 gtcgtcgtat caaggaagaa ggattgcaga ttacagctgt aacaacttct agtgtgacca
     6901 ctaaacaggc agaaggcctt aatatcccgc tcaagtctat tgaccaagta gactttgtcg
     6961 atgtgacggt tgatggcgcg gatgaagtgg atagtcagtt taatggaatc aaaggcggtg
     7021 gtggtgccct tctcatggaa aaggtggtcg caacaccatc aaaagaatac atttgggtgg
     7081 tggatgaaag caagctggtc gaaaaactag gtgcttttaa attgccagta gaagtggttc
     7141 agtatggtgc agagcaggtc tttcgtcatt ttgaacgagc tggctacaaa ccaagtttcc
     7201 gtgaaaaaga cggccaacgt tttgtgacgg atatgcagaa ttttatcatt gacctcgcct
     7261 tggatgtcat tgaaaatcca attgcttttg gacaagaatt ggaccatgtc gttggtgttg
     7321 tggagcatgg tttattcaac caaatggtgg ataaggtaat cgttgctgga cgagatggag
     7381 ttcagatttc aacttcaaaa aaaggaaaat agaagggggc ataagatgtc taaatttaat
     7441 cgtattcatt tggtggtact ggattctgta ggaatcggtg cagcaccaga tgctaataac
     7501 tttgtcaatg caggggttcc agatggagct tctgacacac tgggacacat ttcaaaaaca
     7561 gttggtttga atgtcccaaa catggctaaa ataggtcttg gaaatattcc tcgtgaaact
     7621 cctcttaaga ctgtagcagc tgaaagcaat ccaactggat atgcaacaaa attagaggaa
     7681 gtatctcttg gtaaggatac tatgactgga cactgggaaa ttatgggact caacattact
     7741 gagcctttcg atactttctg gaacggattc ccagaagaaa tcctgacaaa aatcgaagaa
     7801 ttctcaggac gcaaggttat tcgtgaagcc aacaaacctt attcaggaac ggctgttatc
     7861 gatgattttg gaccacgtca gatggaaact ggagagttga ttatctatac ttcagctgac
     7921 cctgttttgc agattgctgc ccacgaagac attattcctt tggatgaatt gtaccgtatc
     7981 tgtgaatacg ctcgttcgat tacccttgag cgtcctgccc ttcttggtcg catcattgct
     8041 cgcccttatg taggtgaacc aggtaacttc actcgtacgg caaaccgtcg tgacttggct
     8101 gtatctccat tttccccaac tgttttggat aaattgaatg aggctggtat cgatacttat
     8161 gctgtgggta aaatcaacga tatctttaac ggtgctggta tcaaccatga catgggtcac
     8221 aacaagtcaa atagtcatgg aattgataca ctattgaaga ctatgggact tgctgagttt
     8281 gaaaaaggat tctcattcac aaacctagtt gactttgatg ccctttacgg ccatcgtcgt
     8341 aatgctcacg gttaccgtga ttgcttgcat gagtttgatg aacgcttacc tgaaattatc
     8401 gcagctatga gagagaatga ccttctcttg attactgcgg accatggaaa tgacccaacg
     8461 tatgcaggaa cggatcacac tcgggaatat attccattgt tggcctatag ccctgccttt
     8521 aaaggaaatg gtctcattcc agtaggacat tttgcagata tttcagcgac tgttgccgat
     8581 aactttggtg tggaaactgc tatgattggg gaaagtttct tagataaatt ggtataagat
     8641 gacgcgctat gctttgctgg tgagaggtat caatgttggt ggtaagaata aggtcgtcat
     8701 ggcggaactt cgtcaagaat tgacaaactt gggactggaa aaggttgaga gctacatcaa
     8761 cagtggcaat attttcttta cttcgataga ttccaaagcc caattggttg aaaagctaga
     8821 gactttcttt gcagtccatt atccatttat tcagagcttt tctttactga gtctagagga
     8881 ctttgaggcg gaacttgaaa atctaccagc ttggtggagc agagacttgg cacgaaaaga
     8941 ttttctcttt tacactgagg gtttggatgt ggaccaagtc atcgcgacag ttgaaagttt
     9001 agagctgaaa gatgaagtgc tttattttgg aaaacttggg attttctagg ggaaattttc
     9061 tgaagaatcc tattctaaga ctgcctatca taagtacttg ctgaaggtgc ctttctaccg
     9121 ccacattact attcgtaatg ctaaaacctt tgacaaaatt ggtcaaatgc taaaaaaata
     9181 ataaaggaga cacacaatga catttttaaa caaaatccat gaaactgcta ctttcctgaa
     9241 agaaaaggga attgcagccc ctgagttcgg tctaatcctt ggatcaggac ttggagaatt
     9301 ggcagaagaa atcgaaaatc cagttgtagt agactatgct gagattccaa actggggccg
     9361 ttcaacagta gtcggtcatg ctggtaaatt ggtatatggt gaactggcag gtcgcaaggt
     9421 cttggctctt caaggtcgtt tccatttcta tgaagggaat cctctggaag tggtgacttt
     9481 cccagttcgt gtgatgaaag ttcttggatg tgaaggtgtt attgtaacca atgcagctgg
     9541 cggtatcgga tttggtcctg gtaccttgat ggctatctca gaccatatca acatgacggg
     9601 gcaaaatcca ttgatgggtg aaaacttgga tgactttggc ccacgtttcc cagatatgtc
     9661 tagggcctac acaccagaat accgtgccac tgcccatgaa gtggctaaaa aacttaatat
     9721 caagcttgat gaaggtgtct atatcggagt tactggtccg acttatgaaa caccagcaga
     9781 aattcgttcc tataagacac tgggagcaga tgcagttggt atgtctacgg ttcctgaagt
     9841 tatcgtggca gcccactctg gcttgaaagt tctgggaatt tcatgtatca ctaactttgc
     9901 ggctggtttc caagaagaac tcaatcacga agaagttgta gaagtgactg aacgtgttaa
     9961 aggtgatttc aaaggcttgc ttaaagcgat tcttgctgaa ttgtaagaaa aaagatttaa
    10021 aagggggagt gcctctgtct tttcaggatt gactgcctat ccgtattaaa gaagaaacag
    10081 aggaatacta tgagcttctt cctgctctta taactgaaag aagcggaaga ataggtatgt
    10141 ctgatctgat agccagcatt gtgaaagaca agattctagg atactagcat tagcttccta
    10201 gccaagcaga ctagtatgat aaggagagat gagaatgaat tgactttctg aatttctcag
    10261 tcttatcata tatagcacaa tgagatttcg cttgagtctg cttgtaaata aacgaaaaga
    10321 aagataagaa ataatgaaaa ttggtcaacg aattatgcgc tttggcataa aaaattaagt
    10381 atcggagttg tatctgttgt agtcggcttt gatttctagc tccagctgga atttcagcca
    10441 atgaagtaaa gcaagatgta acatctgaag tggtaatagg tgtgctagat tctaaggagg
    10501 aattgaaaga gtcagaaaat gatgctccaa aactagaaac tcctcttaga gaggagccaa
    10561 gactagctcc tcaaacgctt ccggaagcaa gtgaagttct tgaaaacaaa agggaagagt
    10621 caaaagtaga gataacagag ccagctcaag cggatgatat ccgcaaggtt gttggggaat
    10681 tagccaagga tataagtatt actaagttgt atatgacagg tcattctctt ggatgctacc
    10741 tagctcagat tgcagaggtt gaagcttacc aaaaatatcc tgatttttat aaccatgtat
    10801 tgaggaaagt gacaactttc agtgctccta aagtgattac ttccagaact gtttggaatg
    10861 ctaagaatgg tttctgggat gttggtttgg aaagtcgtaa attagctgtt agtggaaaaa
    10921 ttaagcatta tgtggttgat aatgacaatg ttgtgactcc cttgattcat aatgatcgtg
    10981 atattgttac atttacaggt aattcacgct ttaaacaccg ttctcgtggc tattttgaaa
    11041 gtccaatgaa tgatattcct aactttaata ttggtaaaca agctaccttg gataaacatg
    11101 gttatcgtga tccgaaattg gataaagtgc gattctttaa gaaacaggct ctgcctcgat
    11161 cttctagtca accaagcgct gaaccaatgg aaaatattgc cttaggaaaa taggttactc
    11221 aaagttcgac agctttcgga ggagatgcta gaagagctgt ggatggcaaa gtcgatggta
    11281 actatggtca caattctgtc actcatacaa actttcaatc taagccttgg tggcaagtag
    11341 atttggctaa agaagaaacc attcgccaaa tcaatattta caaccgaaca gacactgccc
    11401 aggatagatt ggcaaacttt gatgtcattc ttttagacag ttctggtaaa gaaattgagt
    11461 gaaaacgtat aacatctcct aaagatgtgt cagcacaaat tacgattaac cataaaaaag
    11521 cgcgctatgt tcggattgag ctagaaggct ataatgccct cagtcttgca gaagttgaag
    11581 ttttccgctt tatagctacg aatggtgaaa cggcgataca agtttctaag tcagttcaac
    11641 caatcagtca gactcctgtg aaggataaaa cattgacaat tcaacacagt ggagcttaca
    11701 ttgcccgcta ctccataact tgggaagaag ttacagtaga taaagatgga aaccaagttg
    11761 ttcgtagtca ttcttgggaa ggaaacggtc gcaaccagac tgcaggtttt gtcctcaacc
    11821 tcccaatcaa agaaaatatg agaaatctgc gagttaagat tgagaaaaag acgggcctac
    11881 tatggaatag atggcaaaca atctatgaaa acagaccaat tttagctcaa ccccaccgta
    11941 aaattaccca ttggggtacg acattgaatt ccaaggtgag tgacgatgat gtcttgtaat
    12001 ctgatggtac aatgatagtt agtttgtcta gtttataaga aagtactacc tgagcttgaa
    12061 taggactcag gtagctctct gtgaaagaac aaaattaata ctcaatgaaa atcaaagagc
    12121 aaactaggaa actagccgca ggttgctcaa agcactgctt tgaggttgta gataagactg
    12181 acgaagtcag tcacatatat aatccaaggc gacgttgacg tggtttgaag agattttcga
    12241 agagtataaa cagaaaggta gagcgcgtgt tctaatttga acacgagtag aaaacttttc
    12301 taaaaataaa aacgaaagga tgggtaaact gtattcgctg aactgaatac gggcgactct
    12361 cctctaaatc aaaattaaga aagaaaggaa ttgaccccac cctaaaagca gtgggaaaaa
    12421 gatagttggt ctagcgagca tcgctcactg cgcccaactc ctattttccc ttcgcttttt
    12481 gatgggtttg gtatctttct caatataaaa tataaaataa agaaaggtag agcgtgtgtt
    12541 ttgatttgaa cacgagcgga aaactcggaa aatagataat ctgactgaaa aatcaggatt
    12601 tctcgtcagg ttcctaattt tcagtcgttt tcttctcgct ctttgtatca taaattatgt
    12661 ctatccatat tgctgctcag cagggtgaaa ttgctgataa aattcttctt cctggggatc
    12721 ctcttcgtgc taagtttatt gcggagaatt tccttggtga tgctgtttgt tttaacgaag
    12781 tgcgtaacat gtttggttac actggtactt acaagggtca ccgtgtatct gtcatgggaa
    12841 ctgggatggg aatgccatct atttcgattt atgcgcgtga gttaatcgta gactacggtg
    12901 tgaagaaatt gattcgtgtg ggaactgcag gttctttgaa tgaagaggtt catgttcgtg
    12961 aattagtttt ggcgcaggcg gctgcaacca actcaaacat cgttcgtaat gactggccac
    13021 agtacgattt tccacaaatt gctagctttg atttgcttga taaagcctac catatcgcca
    13081 aagaacttgg tatgactact cacgttggga acgttttgtc atctgatgtc ttttactcaa
    13141 attactttga aaagaatatc gagcttggta aatggggagt caaggctgtg gaaatggaag
    13201 cagcagctct ttactatctt gctgcccaat accatgttga tgcgctagct atcatgacca
    13261 tctctgatag cttggtcaat ccagacgaag acacaactgc agaagaacgt caaaatacct
    13321 tcactgatat gatgaaggtt ggtttggaaa ccttgattgc agaataatta tagccaaaaa
    13381 ggaagccctt tatgaattgt aggacttcct tttcttatcc agaaattgat ctagctctct
    13441 ctgatttcga agaatagtga ctttatgtga atattcttgg caaagttttt ggtaattttc
    13501 tttttgagtt ttgctacgcc catcccaaag aatccatctg ataaactccc agtcaaagcg
    13561 ttcagggcaa tctgccgcca tactttctct gacttttcca cggtatttaa gataacgctt
    13621 aaaggctcta aagagacagg tcaatggcaa aaaattgaga aagatgattt ggtcagcttc
    13681 ttgcattcgt tcttggtagt agcaccaaga ataattacca tcgatgaccc aagctttatg
    13741 cttggtgaga aagtttttta tctcggttaa catccattcg cagtcactgt cttgccaacc
    13801 aggttgaaat tggagtgtgt ccatgtgcag ttttggaatg gagtagtagt tagataactt
    13861 ttctgctaga gttgacttac cagaaccaga atatccgata attgtgattt tcattttcta
    13921 ccttttccta tttggagaca aaaaaacagc ctctatggac tgtttcttat ttagcaagtt
    13981 tagctgaaag acgagcttta tcgcggcttg ctttgttttt gtgaatcaaa cctttagttt
    14041 ctgctttatc gatagctgag ctagcagcac ggaaaagttc ttcagatggg tttgcttcga
    14101 aagcttttat agcagtacgc atagctgatt tttgagctga gttcttttcg ttttgtttaa
    14161 cgttcaattc agcgcgtttg atagctgatt taatgtttgc caatgttctt acctccatat
    14221 ttactaacta taccattata tatgaaaact tacgttttga caagggaaaa ttattttttt
    14281 aagtaaattt catcgatttc atggttcttg cttttatgaa gaatcacttc tgcacgattt
    14341 ctggttggtt caatataatt ttgtagattt gtgagattga tactggtcca gacctgatgg
    14401 gcaaaggctt ccacttcccc aatcggcatc tgagtaaaac gataatagta gctatcaggg
    14461 tcgttttggg ctaggctcag cattttcaag aaacggtcca gataccaact ttcaatatca
    14521 tccactccag catctacata gatggaaaag tcaaagaagt cagtgatata gagacgatcg
    14581 ttttgtggat tttgaaagac attgattccc tcaacaatta caaaatcagc agctttgaca
    14641 ctttgtttct cttcgggtac gatgtcgtaa acttcatgag aatagacagg aatatctaca
    14701 tcttgtccat ttttgatgcg gtccaagaag ttgagaagag cttccatatc atagctttca
    14761 ggaaatcctt tacgatttaa aatcccctgc tcaatcaagg tttgattggg atagagaaaa
    14821 ccatcagttg taaccaactc aaccgtagca tctgtaaacg tacgggacag taggatttga
    14881 agtaggcgac tggttgtgga ttttccaacg gcaacactcc cagaaacccc aataataaaa
    14941 ggttgagatt tactttcacg ttggaggaaa attccttttg aaaaggctaa atcttcctta
    15001 gttcgcttgt aaatctgaat cagatgagcc aaggggagat agatatctgt aacgtcttgg
    15061 agactgattt ggtcattaaa actcttgata gattccaatt cttcttctgt caaaggaggt
    15121 gttgtctttc gatgtaaaga ttgccaagtc tggcggctga ttttttcaaa atgtaaaaat
    15181 tcgttggtca tttgttttcc cctagatatt ttgtctatca taccataaaa atctaaaaaa
    15241 ataaagcggt ttcttgaatg tggtaagtca atggtatata gtataagtga tcaatttatt
    15301 gatgaaatag gagatagatt ctactgattt ataagtttat aagcttataa agggggcttt
    15361 ttagttcaca acttatagga ggtgtactat gaaaatctta aaacgttaca tattggaact
    15421 ctgttttatt ttaagttttg ctttaccttt tataaaagga gccaatgcag ataatggtat
    15481 atgctttgtg gaaacctatt acggttttac ttttttgatg gaacatgcta ttgtaacagc
    15541 tgtctttatc tattcgtttt taattgcttt cttactaaaa aaacgatggg cgaaatggat
    15601 tgctgctggt agttattgct ttttagtcct atggattgcg acagagggtt atttcttccg
    15661 catgtcacta gaagatttga tacgactttg gacaagtttg gaaatcctga cacaaacgta
    15721 tcagttgggc ttttatctaa acattttgtt aggaattttg ttgataataa agtattttaa
    15781 ggttaagcaa tagaaataat aatctgcctg attatagatg ataaactttt tgtttttatc
    15841 tggactgtga tatttatagt tctaaatggt ttattattta taagtgttga aagagattga
    15901 agaaaatggc ttcgtgactt gctttcttcg gtctttttcg ttttattatc aatttgtaaa
    15961 cgatttacaa tttgagtcaa attatggtaa aatagtgtca tgagtaaaat gtattatgca
    16021 gaaaatcctg acgctgctca cgacattcat gagttgagag tagagttgtt gggacaaaaa
    16081 atgacctttt taacggatgc gggtgttttt agcaagaaaa tagttgactt tggaagtcaa
    16141 ctattgctca agtgtcttga ggtcaaccaa ggagaaactg tccttgatgt aggctgtggt
    16201 tacggacctt tgggtttgtc cttagctaag gcttatggag ttcaggctac tatggttgat
    16261 atcaacactc gtgccttgga tttagctcgg agaaatgctg aaaaaaataa tgcaaaagcg
    16321 acgattttcc agtccaacat ctatgaacaa gttgaaggac attttgatca tgtcatttcc
    16381 aatcctccta tccgtgcggg caagcaagtc gttcatgaaa tcattgagaa aagtaaagat
    16441 ttcttggaaa ctggtgggga tttgacaatc gtcatccaga aaaaacaagg ggctccaagt
    16501 gctaagtcca agatggagga tgtgtttggt aattgtgaaa tccttaaaaa agacaaggga
    16561 tactatatcc ttagaagtgt gaaagaatga gagcagttga tttaatacaa aaaaaacgag
    16621 acggtcaaga actgacttca agtgaaattg aatggctggt agaaggctat gtgtcaggaa
    16681 ctgttcctga ttatcagatg tctgcctttg ctatggctgt ttattttaaa ggaatgacga
    16741 ctcgtgaaat ttctgattta acgatgaata tggttaagac tgggcaagag tttgacctgt
    16801 cagccattga tggtgtgaaa gttgacaagc attctactgg tggtgtcggc gataaggtaa
    16861 ccttgattct agctcctctt gttgctagct tcggtgtgcc tgtagcaaaa atgagtggtc
    16921 gtggtcttgg ccatacaggc ggaacaattg ataaattgga gtccattaag ggctatcaag
    16981 tagaacgtag tcaagaggat tttattcgtc aggttcagga tattggagta tctgtcattg
    17041 ggcaatcaga ccagctggtc aaagcagata agcttctcta tgcccttcgt gatgtgaccg
    17101 caactgtcga cacgattcct ttgattgcga gttcggtgat gagcaagaaa attgcggcag
    17161 gggcggatgc tattttgcta gacgtgactg tcggtgaggg tgccttcatg aagacggttg
    17221 atgaggcgcg tgagttggct caaactatgg tagatcttgg taaggttgtt ggacggaaga
    17281 cagtagcagt gattacggat atgagccagc ctttgggacg agcgattgga aatcgtctgg
    17341 aaatccttga agcattggag attttgcaag gtcaaggacg tcaggatatt acccacttta
    17401 tttgtgaatt ggctcaaatt atgcttggtt tggcaaatgt taacaagact gttgaagaag
    17461 ttcgccaaca tcttgagaat ggtcaagcac tggctaagtt tgaggaaatg gtgcaagccc
    17521 aaggtggaga cttggaagac ctctatcgtc ctgtaaatgt agctcatgtg gtggaaatcc
    17581 ctgctcagga aacgggtgtc atttcagctc ttccagctat ggactttggt ctttatgcca
    17641 tgaggctggg tgctggtcgt gcagttaagt ctgatgcctt ggactatgaa acaggaattg
    17701 tttttgaaaa gaagattgga gactccgttc aaaagggtga aattgttgca aaagtataca
    17761 caaatgaaaa aattccacct caactagtta cagatttcca aaaatgtgtt aaaataagtg
    17821 atgaagtgaa aaaaatccgt gaaattgtag aaatcatctc ctaaatggag ggaaaaccga
    17881 aatgaaatta aataaatata tagatcatac gcttttaaaa caagatgcaa agaaaaaaca
    17941 aattgatagt ttgttgtctg aggctagaga gtatgacttt gccagtgttt gcgttaatcc
    18001 gacctgggtt gaacatgcta aaaaaggact tgaaggcaca gatgttaagg tttgcacagt
    18061 agtaggtttc cctttgggag caacaacttc agccgtgaaa gcatttgaga caaaagaagc
    18121 tatccaaaat ggtgcagatg agattgatat ggtgatcaat gttggagctc tcaaatcagg
    18181 taatttagcc ttggttgagt cagatattcg cgcagtagtg gaagcaagtg gtgataagtt
    18241 agtgaaagtc attattgaag cttgccttct gacagaccaa gaaaaaattg ttgtttgcca
    18301 attggcccaa aaagctgggg ctgactttgt taaaacatct actggttttt caactggtgg
    18361 tgctacgata gcagatgtta cattaatgcg tgaaacagtt ggatctgata tgggagtcaa
    18421 ggctgctggt ggagctcgtt cttatgcaga tgctcttgcc tttgtcgaag caggtgcgac
    18481 ccgtatcgga acgtcagctg gggtagctat tttaaaagga gaattggcag atggcgacta
    18541 ctgagttgat tgaactggca attgaaacca gcaaacatgc ctatgtcccc tattctcact
    18601 ttcctatcgg agcagtttta gtagccaaag acggaaatgt ttacacggga gtgaatatcg
    18661 aaaatgctag ctatcctttg accaattgcg gtgagagaac agctattttt aaggctatat
    18721 ctgaaggcca aagagaattt tcagaattga ttgtctatgg tcaaactgaa aaaccgattt
    18781 caccatgtgg tgcttgtcgc caagtaatgg tcgaattttt tgaacaagat ttaaaagtga
    18841 ccttagtcgc aaaagataaa tcgacggtcg agatgacggt cggggagtta cttccatatt
    18901 cttttacaga cttaaattag tctgagtcgc tctttgagtg gcaagggtct ttgtgaccaa
    18961 tcaatccata cttgcaacat cgttgcacat cttatttagg aggttcagta atgaacaaga
    19021 aacaatggct aggtcttggc ctagttgcag tggcagcagt tggacttgct gcatgtggta
    19081 accgctcttc tcgtaacgca gcttcatctt ctgatgtgaa gacaaaagca gcaatcgtca
    19141 ctgatactgg tggtgttgat gacaaatcat tcaaccaatc agcttgggaa ggtttgcagg
    19201 cttggggtaa agaacacaat ctttcaaaag ataacggttt cacttacttc caatcaacaa
    19261 gtgaagctga ctacgctaac aacttgcaac aagcggctgg aagttacaac ctaatctttg
    19321 gtgttggttt tgcccttcac aatgcagttg aagaagcagc taaagaacac actgacttga
    19381 actatgtctt gattgatgat gtgattaaag accaaaagaa tgttgcgagc gtaactttcg
    19441 ctgataatga gtcaggttac cttgcaggtg tggctgcagc aaaaacaact aagacaaaac
    19501 aagttggttt tgtaggtggt atcgaatctg aagttatctc tcgttttgaa gcaggattca
    19561 aggctggtgt tgcgtcagta gacccatcta tcaaagtcca agttgactac gctggttcat
    19621 ttggtgatgc ggctaaaggt aaaacaattg cagccgcaca atacgcagcc ggtgcagata
    19681 ttgtttacca agtagctggt ggtacaggtg caggtgtctt tgcagaggca aaatctctca
    19741 acgaaagccg tcctgaaaat gaaaaagttt gggttatcgg tgttgaccgt gaccaagaag
    19801 cagaaggtaa atacacttct aaagatggca aagaatcaaa ctttgttctt gtatctactt
    19861 tgaaacaagt tggtacaact gtaaaagata tttctaacaa ggcagaaaaa ggagaattcc
    19921 ctggcggtca agtgatcgtt tactcattga aggataaagg ggttgacttg gcagtaacaa
    19981 acctttcaga agaaggtaaa aaagctgtcg aagatgcaaa agctaaaatc cttgatggaa
    20041 gcgtaaaagt tcctgaaaaa taatggatgg aagtcatttc ttaggaagcg gccaagggcc
    20101 gcttttttaa gagttgagac aaagtcattt tgtctcagta aagcttgcta ctagacaaac
    20161 tagcaagttt tattgaaata aaataagact ctgaaaggaa gagcacatgg cacacgaaaa
    20221 tgtcattgag atgcgtgata ttaccaaggt gtttggtgga tttgttgcca acgacaaaat
    20281 caacttgcac ctacgaaaag gtgaaattca tgcactttta ggagaaaatg gggctggtaa
    20341 gtccacgcta atgaacatgt tagcagggct tcttgaacca actagtggtg aaatcgcggt
    20401 caacggtcaa gttgtcaatc tcgactcccc atctaaagca gctagcttgg gaatcgggat
    20461 ggttcaccag cactttatgt tggttgaagc cttcacagtg gctgaaaaca tcattttagg
    20521 tagtgaattg actaaaaatg gtgtgctaga tatcgctgga gctagcaaag aaatcaaggc
    20581 tctttctgaa cgttatggct tagctgttga cccttctgcc aaggtagcag atatctcagt
    20641 tggagcccaa caacgtgtag aaattttaaa aacactttat cggggggctg atatccttat
    20701 ctttgacgaa ccaacggctg ttttgactcc atcagaaatt gatgagttga tggctattat
    20761 gaaaaatctt gtcaaagaag gaaaatcaat tatcttgatt acccacaaat tggatgaaat
    20821 tcgcgcagtt tctgaccgtg ttacagttat ccgtcgtggg aaatcaattg aaaccgttga
    20881 aattgcaggg gctaccaatg ctgatttggc ggaaatgatg gtagggcgtt ctgtttcctt
    20941 taaaacagag aagcaagcct ctaaaccaaa agaagtggtt ttgtctatca aagatttggt
    21001 ggtcaatgaa aaccgtggtg ttccagctgt taaaaatctg tccttggatg ttcgtgctgg
    21061 agagattgtt ggtattgcgg ggattgatgg aaatggtcag tctgaactga ttcaagccat
    21121 tacaggtctt cgtaaggttg aatctggtag cattgagcta aaaggagatt caattgtagg
    21181 cttgcaccca cgtcagatta cagaactaag tgttgggcac gttccagaag accgtcaccg
    21241 tgatggcttg attttggaaa tgatgatatc tgaaaatatt gcccttcaaa cctactataa
    21301 agaaccacat agtaaaaatg gaattttgaa ttattcaaat attacttctt atgctaaaaa
    21361 gctgatggaa gagtttgatg ttcgcgctgc cagtgaatta gttcctgcag ctgcactctc
    21421 aggaggaaat caacaaaaag caattattgc tcgtgaaatt gatcgagatc ctgatctcct
    21481 tatcgttagc cagccaactc gtgggttgga tgtcggtgcc attgagtata tccacaaacg
    21541 cttgattgaa gagcgtgata atggcaaggc tgtccttgtt gtcagctttg aattggatga
    21601 gattttaaac gtctcagacc gtattgccgt tatccacgat ggtaagattc aaggtattgt
    21661 atcaccagaa acaaccaata aacaagaact tggtgtcttg atggctggtg gaaacttggg
    21721 aaaggagaag agtgatgtct aaaaaattac aacaaatttc ggttcccttg atttctgtat
    21781 ttctaggaat tttactcgga gccattgtca tgtggatctt cggttatgat gctatttggg
    21841 gctacgaaga attgttctat acagcctttg gcagtctgcg tgggattgga gaaatcttcc
    21901 gtgctatggg tcctctggtc ttgattggtc ttggttttgc ggttgccagt cgagctggtt
    21961 tctttaacgt cggacttcct ggtcaggctt tggcaggttg gattctcagt ggttggtttg
    22021 cgctgtcgca tccagatatg ccccgtccct tgatgattct agcaaccatc gtgattgcct
    22081 tgattgctgg tgggattgtc ggagcgattc caggtattct tagggcctat ctagggacgt
    22141 cagaggttat tgtaaccatc atgatgaact acattgtctt gtatgtaggg aatgccttta
    22201 tccatgcttt ccctaaagac ttcatgcaaa gtacagattc gaccattcgt gttggggcta
    22261 atgcaactta tcagacacct tggttggctg agttgactgg taactcacgg atgaatattg
    22321 gtattttctt tgccatcatt gccgttgcag ttatttggtt catgctcaag aaaacaactc
    22381 ttggttttga aatccgtgca gttggtctta atccacatgc ttcagaatat gctggtattt
    22441 ctgccaagcg gactattatc ctatctatga ttatttcagg tgccttggca ggtcttggtg
    22501 gagctgttga aggtttggga accttccaga acgtctatgt tcaaggttcg tcattagcta
    22561 tcggatttaa cggaatggcg gttagtttgc ttgcggccaa ctcaccaatt ggtatactct
    22621 ttgcagcctt cctatttggc gttctccaag ttggggctcc tggtatgaat gcggcgcagg
    22681 taccatctga gcttgtcagc attgtaacag cgtctattat cttctttgtc agtgttcatt
    22741 accttatcga acgctttgtc aaaccgaaaa aacaagttaa aggaggtaag taaagatgtc
    22801 tattataacc ttgctccccc tcttggtgtc ttctatgctg atttactcag cacctctcat
    22861 ctttacaagt atcggtggtg ttttctctga acgtggtggt gtggtaaacg ttggccttga
    22921 aggaattatg gttatgggtg ccttttctgg agttgtcttt aaccttgaat ttgcagaaca
    22981 atttggagca gcaactccat ggctatcctt gcttgtagca ggattggttg gtagtgtttt
    23041 ctctatcatc cacgcagcag cgacggttca tttccgtgca gaccatgttg tcagcggtac
    23101 ggtattgaac ttgatggcgc ctgccttggc tgttttctta gttaaagttc tttataacaa
    23161 aggacaaacc gacaacctaa gtcaaacttt tggacgcttt gatttcccag tcttggcaaa
    23221 tatcccagtg attggtgata tcttcttcaa gtcaactagt ctacttggtt atctggcgat
    23281 tgccttctca ttccttgctt ggtttattct ctttaagact caatttggtc ttcgtctccg
    23341 ctctgtcggt gaacatcctc aagcagcgga taccttggga atcaacgtct acaagatgag
    23401 atatttaggg attattattt caggttttct aggtggaatt ggcggagcga tttatgcgca
    23461 atcaatctca gttaacttct cagtgacaac tattgttgga cctggattta tcgcccttgc
    23521 tgcgatgatc tttgggaaat ggaatccaat cggtgctatg ctttctagtc tcttctttgg
    23581 actttcacaa agtttggctg ttatcggttc tcaattgccg ttcctacaag gagtgccagc
    23641 ggtttatctt caaattgcac cttatgtttt gacaattctt gtcttagcag ccttctttgg
    23701 aaaagcagtc gcaccaaaag cagatggtat caactacatc aaatcaaaat aagcatacaa
    23761 aaaaacgtca gtccaatgac tgaactgccc cccaaaagtt agacagaaaa aatctaactt
    23821 ttggggtgtt tttattatga aa
//
LOCUS       DAVBNO010000028        23291 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVBNO010000028 DAVBNO010000000
VERSION     DAVBNO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23291
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.028"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            60..929
                     /locus_tag="VQ846_001226"
     CDS             60..929
                     /locus_tag="VQ846_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET3075804.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILGISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            933..1889
                     /locus_tag="VQ846_001227"
     CDS             933..1889
                     /locus_tag="VQ846_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET3075805.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1889..2653
                     /locus_tag="VQ846_001228"
     CDS             1889..2653
                     /locus_tag="VQ846_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075806.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2653..3363
                     /locus_tag="VQ846_001229"
     CDS             2653..3363
                     /locus_tag="VQ846_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075807.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQETTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3671..4327
                     /locus_tag="VQ846_001230"
     CDS             3671..4327
                     /locus_tag="VQ846_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET3075808.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            4435..5530
                     /gene="prfB"
                     /locus_tag="VQ846_001231"
     CDS             join(4435..4506,4508..5530)
                     /gene="prfB"
                     /locus_tag="VQ846_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET3075809.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5548..6240
                     /gene="ftsE"
                     /locus_tag="VQ846_001232"
     CDS             5548..6240
                     /gene="ftsE"
                     /locus_tag="VQ846_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET3075810.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6233..7159
                     /locus_tag="VQ846_001233"
     CDS             6233..7159
                     /locus_tag="VQ846_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3075811.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            7445..9625
                     /locus_tag="VQ846_001234"
     CDS             7445..9625
                     /locus_tag="VQ846_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET3075812.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDTSQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKNLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            9677..10492
                     /locus_tag="VQ846_001235"
     CDS             9677..10492
                     /locus_tag="VQ846_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET3075813.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            10632..11975
                     /locus_tag="VQ846_001236"
     CDS             10632..11975
                     /locus_tag="VQ846_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET3075814.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIADSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQSMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            12352..13542
                     /locus_tag="VQ846_001237"
     CDS             12352..13542
                     /locus_tag="VQ846_001237"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET3075815.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKAKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            14095..15030
                     /locus_tag="VQ846_001238"
     CDS             14095..15030
                     /locus_tag="VQ846_001238"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET3075816.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            15064..16101
                     /locus_tag="VQ846_001239"
     CDS             15064..16101
                     /locus_tag="VQ846_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET3075817.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            16274..16879
                     /locus_tag="VQ846_001240"
     CDS             16274..16879
                     /locus_tag="VQ846_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET3075818.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            17035..17565
                     /locus_tag="VQ846_001241"
     CDS             17035..17565
                     /locus_tag="VQ846_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET3075819.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            17586..18671
                     /gene="rlmN"
                     /locus_tag="VQ846_001242"
     CDS             17586..18671
                     /gene="rlmN"
                     /locus_tag="VQ846_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET3075820.1"
                     /translation="MKPSIHSLTHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKSSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGT
                     DIDAACGQLLFNTMKRDRQKAVAAVNP"
     gene            18671..19153
                     /locus_tag="VQ846_001243"
     CDS             18671..19153
                     /locus_tag="VQ846_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET3075821.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            19155..20696
                     /locus_tag="VQ846_001244"
     CDS             19155..20696
                     /locus_tag="VQ846_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET3075822.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQTKNLQIGY
                     DLPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(20752..21555)
                     /locus_tag="VQ846_001245"
     CDS             complement(20752..21555)
                     /locus_tag="VQ846_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET3075823.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(21638..21859)
                     /locus_tag="VQ846_001246"
     CDS             complement(21638..21859)
                     /locus_tag="VQ846_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075824.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            complement(22338..22547)
                     /locus_tag="VQ846_001247"
     CDS             complement(22338..22547)
                     /locus_tag="VQ846_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075825.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            22717..22989
                     /gene="rpsP"
                     /locus_tag="VQ846_001248"
     CDS             22717..22989
                     /gene="rpsP"
                     /locus_tag="VQ846_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET3075826.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            23009..23248
                     /locus_tag="VQ846_001249"
     CDS             23009..23248
                     /locus_tag="VQ846_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET3075827.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
BASE COUNT         6969 a         4224 c         5092 g         7006 t
ORIGIN      
        1 agaacctatc aaaaagtgag ggaaacccct cggaattata aatagaaaga gtgaatctta
       61 tgctccaaca actcgtaaat ggtttgattc taggtagtgt ttacgcgctg ttagccctag
      121 gatataccat ggtttacgga attatcaagc tcatcaactt cgcccatggt gatatttata
      181 tgatgggagc ctttatcggt tatttcttga tcaattcttt ccaaatgaat ttctttgtag
      241 cgcttattgt agctatgcta gcgacagcta ttcttggtgt cgtgattgag tttcttgctt
      301 accgaccttt gcgccactct actcgtattg ctgttttgat tacggctatt ggggtttctt
      361 tcctattgga gtatggaatg gtctatctgg ttggtgccaa tacccgtgcc ttccctcaag
      421 cgattcaaac agttcgatat gatttgggac caattagctt aacaaatgtg cagttaatga
      481 ttttgggcat ttccttgatt ttgatgattt tgttacaagt cattgtccaa aagactaaga
      541 tggggaaagc catgcgtgca gtatcagtag atagcgacgc ggcgcaattg atggggatca
      601 atgtaaaccg tacgattagc tttaccttcg ctttgggttc tgctcttgcg ggtgcggctg
      661 gtgttctgat tgctctttat tataactctc ttgagccttt gatgggggtt actccaggtc
      721 ttaaatcttt cgttgccgca gtacttggtg gtatcggaat tattcctggt gcggctcttg
      781 gtggctttgt gattggtcta ttggaaacct ttgcgactgc ctttgggatg tcagatttcc
      841 gtgatgccat tgtttatgga atcttgttgt tgatcttgat tgtccgccca gctggtatcc
      901 ttggtaagaa tgtgaaagag aaggtgtaaa cgatgaagga aaatttaaaa gttaatattc
      961 tatggttact ccttttgtta gctggctata gcttgattag tgtactggtt tcagtcggag
     1021 tacttaatct attctatgta cagattttac aacaaattgg aattaatatt attttggctg
     1081 ttggtctcaa cttaatcgtt ggtttttcag gacaattttc acttggtcat gctggtttca
     1141 tggcgattgg tgcctatgca gcagctatta ttggttctaa atcaccaacc tacggtgcct
     1201 tctttggagc tatgcttgta ggggctttgc tttcaggagc agttgcctta cttgtcggca
     1261 ttccaacctt gcgcttgaag ggggactatc ttgcggtagc aactctaggt gtttctgaaa
     1321 ttatccgtat ctttatcatc aatggtggaa gccttacaaa tggtgcggca ggtattttag
     1381 ggattcctaa ctttacaact tggcaaatgg tttacttctt tgtcgtgatt acaaccattg
     1441 caaccttgaa cttcttgcgt agcccaattg gtcgttcaac cctctctgtt cgtgaagatg
     1501 aaatcgctgc tgagtcagtt ggggttaata cgactaaaat taaaatcatc gcttttgtct
     1561 ttggtgccat tactgcaagt attgctgggt cacttcaggc aggatttatc gggtctgttg
     1621 taccgaaaga ttacaccttc atcaactcaa tcaacgtttt gattattgtt gtatttggtg
     1681 gactcggttc cattacaggt gcgattgttt cggctattgt tctgggaatt ttaaatatgc
     1741 ttctccaaga tgttgctagt gtgcgtatga ttatttacgc tttggccttg gtattggtaa
     1801 tgattttcag accaggtgga ctccttggaa cgtgggaact gagcctatca cgtttcttta
     1861 aaaaatctaa gaaggaggaa caaaactaat ggcattactt gaagtaaaac agttaaccaa
     1921 acattttggt ggtctaacag ctgttggaga tgtgactctt gaattgaacg aaggggaact
     1981 ggttggatta atcggtccaa acggagctgg gaaaaccacc cttttcaacc ttttgaccgg
     2041 tgtttatgaa ccaagcgagg gaacagtaac cctagatggt caccttttga atgggaaatc
     2101 accttataag attgcctctt tgggacttgg acgtactttc caaaatatcc gtctctttaa
     2161 agatttaaca gttttagata atgttttgat tgcttttgga aaccatcaca aacagcatgt
     2221 ttttactagt ttcttacgct taccagcttt ttacaagagt gaaaaagaat taaaggctaa
     2281 agctttggaa ttgttgaaaa tctttgattt agatggtgat gcagagactc ttgctaaaaa
     2341 tctttcctac ggacaacaac gtcgtttgga aattgttcgt gcccttgcta cggaacctaa
     2401 aattctcttc ttagatgaac cagcagcagg tatgaaccca caggaaacag ccgaattgac
     2461 tgagttaatt cgtcgtatca aagatgaatt taagattaca atcatgttga ttgaacacga
     2521 tatgaatctg gtcatggaag taacagaacg tatctacgta cttgaatatg gccgtttaat
     2581 tgctcaagga actccagacg aaattaagac caataaacgc gttatcgaag cttatctagg
     2641 aggtgaagcc taatgtctat gttaaaagtt gaaaatcttt ctgtgcatta cggtatgatc
     2701 caagcagtcc gtgatgtaag ctttgaagtt aatgaaggag aagttgtttc ccttatcggt
     2761 gccaacggtg caggtaagac aactattctt cgcaccttgt caggtttggt tcgaccaagt
     2821 tcaggaaaga ttgaattttt aggtcaagaa atccaaaaaa tgccagctca gaaaatcgtg
     2881 gcaggtggtc tttcacaagt tccagaagga cgccacgtct ttcctggctt gactgttatg
     2941 gaaaatcttg aaatgggagc tttcttaaag aaaaatcgtg aagaaaatca agcaaacttg
     3001 aagaaggttt tctcacgctt tcctcgtctt gaagaacgta agaaccaaga tgcagctact
     3061 ctttcaggag gggaacaaca aatgcttgcc atgggacgcg ctcttatgtc aacaccaaaa
     3121 cttcttcttt tagatgaacc atcaatggga cttgccccaa tcttcatcca agagattttt
     3181 gatatcattc aagatattca gaagcaagaa acaaccgtcc tcttgattga acaaaatgcc
     3241 aataaagcac tcgcaatctc tgaccgagga tatgtattgg aaacaggaaa aatcgtccta
     3301 tcagggacag gaaaagaact cgcttcatca gaagaagtca gaaaagcata tctaggtggc
     3361 taaaacaatc cagtggattg ttttagtcgg cagatgaaga ttacgaagta atcatcaata
     3421 tagtccgggg gaccttttta gtcggtagat tgagattgca aacaaatatg catctacatt
     3481 gaaagcttaa tttctaataa ttgaaaaaat cgaatgaaaa atttcttacc ttcattcaca
     3541 gagctctatt tcagagctct ttttgctagc ttattcatac ttttctgaat ttcgaaaaag
     3601 aaatgtaagc gtttgataga tttacaaaaa gattgtataa tagggataag aatagaaaag
     3661 gagaagtctc atggcagtta aagattttat gacccgcaag gtagtttata ttagtccaga
     3721 tacaacagta tctcatgcag cagatttgat gagagagcaa ggtttgcacc gtctgcctgt
     3781 tatcgaaaat gatcaattag ttggtttggt gactgaggga accattgcac aagcaagtcc
     3841 atctaaagca acaagtcttt ctatctatga gatgaattat cttctgaata agacaaaagt
     3901 aaaagatgtc atgattcgcg atgttgtcac tgtctcaggc tatgctagtc tagaagatgc
     3961 aacttatctg atgttgaaaa ataagattag tattctccct gtcgtagata accatcaagt
     4021 atacggagtt attactgacc gtgacgtttt ccaagccttt cttgaaattg caggttatgg
     4081 cgaagaaggg attcgtgtac gctttgttac agaagatgaa gttggtgttc ttggaaaaat
     4141 tgtttctttg attgtagaag aaaatttgaa tatctcccat acagtcaata ttccgcgtaa
     4201 ggatggtaag gtgattatcg aagtgcaaat cgatggatca attgatttac cagccttgaa
     4261 agaaaaattt gaagcaaatg gtattcaagt ggaagaaatc gctcgcactt cagcaaaagt
     4321 cttgtaagaa gggaagccca aaggcttctt ttttcatgaa aaggggatta gagcaaaaga
     4381 tggaaagaaa tgataaaata tgctataatg aaataatgta aaaaaggagt atttatggac
     4441 atttcagtaa ttcgtcagaa aattgacgca aatcgtgaaa aattagcttc tttcaggggg
     4501 tctctttgac ctcgaagggc tagaggaaga gattgccatc ttggaaaaca agatgacaga
     4561 acctgatttt tggaacgata atattgcggc ccaaaaaacg tcgcaagaat tgaatgaatt
     4621 aaaaaacact tacaatacct tccataagat ggaagagttg caggatgaag tcgaaatttt
     4681 attggatttt ttggctgaag acgagtcagt gcatgatgaa ctggtagcgc agttagccga
     4741 acttgataag ataatgacca gctacgagat gactctacta ttgtcagaac cttatgacca
     4801 caacaatgcc atcttggaaa tccatccagg ttctggtggt actgaggcgc aggactgggg
     4861 tgatatgttg cttcgtatgt atactcgtta tggtaatgct aaaggcttta aagtggaagt
     4921 gttggattac caagcaggtg atgaggctgg tattaagtcg gtaactttat catttgaagg
     4981 gcctaatgcc tatggtctcc tcaagtcaga aatgggtgtt catcgcttag tgcgaatctc
     5041 accatttgac tctgccaaac gtcgccatac ctctttcaca tctgtagaag tgatgccaga
     5101 attggatgat actattgaag tggaaatccg tgaagatgat atcaagatgg ataccttccg
     5161 ttcaggtggt gccggtggac aaaacgtcaa taaggtttca acaggtgtac gtttaaccca
     5221 cattccaact ggaattgttg tccaatcaac agtggatcgt acccagtatg gaaatagaga
     5281 tcgtgccatg aagatgttgc aggctaagct ctatcaaatg gagcaagata agaaggctgc
     5341 ggaggtagat tctctcaaag gtgagaaaaa ggagatcact tggggaagcc aaatccgttc
     5401 ttatgtcttc acgccttata ctatggtaaa agatcaccga actagctttg aggttgctca
     5461 ggtagataag gttatggatg gggacctaga tggttttatc gatgcttatc tcaagtggcg
     5521 aattagctaa gatagaaagg aactcacatg tcaattattg aaatgagaga tgtcgttaaa
     5581 aaatacgaca acggaacgac tgctctacgc ggtgtttcgg ttagcgttca accgggggaa
     5641 tttgcttaca tcgtaggacc ttcaggagca gggaagtcaa cttttattcg ttctctgtat
     5701 cgtgaagtaa aaatcgataa aggaagtcta tcagttgctg gttttaatct ggttaagatc
     5761 aaaaagaaag atgtcccgct tctacgtcgt agtgttgggg ttgtcttcca agattataaa
     5821 ttgttaccaa agaaaactgt ctatgaaaat attgcttacg ctatggaagt aatcggggaa
     5881 aatcgccgta atatcaaaag acgagtgatg gaagttttgg acttggttgg attgaagcat
     5941 aaggttcgtt ctttcccaaa tgaactctca ggtggggagc agcagcgaat tgcgattgcg
     6001 cgtgcaattg taaataatcc caaagtattg atagctgatg agccaacagg aaatctggat
     6061 ccggataatt catgggaaat tatgaatctc ttggaacgga ttaacctaca agggacaact
     6121 attttgatgg cgactcataa tagccagatt gtaaatacct tgcgccaccg tgtcattgcc
     6181 attgaaaatg gccgtgtcgt tcgtgacgaa tcaaaaggag agtatggata cgatgattag
     6241 tagatttttt cgccatttat ttgaagcctt aaaaagtttg aaacgaaatg gttggatgac
     6301 agtagctgct gtcagttcag tcatgattac tttgaccttg gtggcaatat ttgcatctgt
     6361 tattttcaat acagcgaaat tagctacaga tattgaaaat aatgtccgtg tagtagttta
     6421 tatccgaaag gatgtggaag ataatagtca gacaattgaa aaagaaggtc aaactgttac
     6481 aaataatgac taccacaagg tatatgattc tttgaagaac atgtctacgg ttaaaagtgt
     6541 taccttttca agtaaagaag aacaatatga aaaattaacc gagataatgg gagataactg
     6601 gaaaatcttt gaaggagatg ccaatcctct ctatgatgcc tatattgtag aggcaaacac
     6661 tccaaatgat gtaaaaacta tagccgaaga tgctaaaaaa attgaaggtg tctctgaggt
     6721 tcaagatggc ggtgccaata cagaaagact cttcaagtta gcttcattta tccgtgtttg
     6781 gggactaggg attgctgctt tgttaatttt tatcgcagtt ttcttgattt caaataccat
     6841 tcgtattacc attatttccc gcagtcgcga aattcaaatc atgcgcttgg ttggagctaa
     6901 aaacagttat atccgtggac cgttcttgtt agaaggagcc tttatcggtt tattgggagc
     6961 tatcgcacca tctgttttgg tctttattgt ttatcaaatt gtttaccaat ctgtcaacaa
     7021 atcgttggta gggcaaaatc tatccatgat tagtccagat ttatttagtc cgttgatgat
     7081 tgccctacta tttgtgattg gggttttcat tggttcattg ggatcaggaa tatccatgcg
     7141 ccgattcttg aagatttagg taaaatagct gcttttatga ggagattgta aaatctcctt
     7201 ttttgctaca agagtttttg aaaagagatg cgtagaagaa aagagcttcc aaagaagtcc
     7261 cccagagaag acttcttgat ggctaatttg aacttgaaat ttggcgcact agaaattttt
     7321 tagaaaaaag tgtaattttt ttataaaagc ctttacaaaa aaaattaaag tggtataatt
     7381 aaatcataga aggtgcaaac gttttcgtaa aacgtttgcc taaataaaaa taaaggagat
     7441 ttgaatgatg aaagatacat tcaaaaatgt cttgtctttc gaattttggc aaaaattcgg
     7501 taaggctttg atggtagtta tcgcggttat gccggctgct ggtttgatga tttcaatcgg
     7561 taagtctatc gtgatgatta acccaacctt tacaccactt gtcatcacag gtggaattct
     7621 tgagcaaatc ggttgggggg ttatcggtaa ccttcacatt ttgtttgccc tagccattgg
     7681 aggaagctgg gctaaagaac gtgctggtgg tgctttcgcc gctggtcttg ccttcatctt
     7741 gattaaccgt atcactggta caatttttgg tgtatcaggc gatatgttga aaaatccaga
     7801 tgctatggta actactttct ttggtggttc aatcaaagtt gctgattact ttatcagtgt
     7861 tcttgaagct ccagccttga acatgggggt attcgtaggg attatctcag gttttgtagg
     7921 ggcaactgct tacaacaaat actacaactt ccgtaaactt cctgatgcac tttcattctt
     7981 caacgggaaa cgtttcgtac catttgtagt tattcttcgt tcagcaatcg ctgcaattct
     8041 acttgctgct ttctggccag tagttcaaac aggtatcaat aacttcggta tctggattgc
     8101 caactcacaa gaaactgctc caattcttgc accattcttg tatggtactt tggaacgttt
     8161 gctcttgcca tttggtcttc accacatgtt gactatccca atgaactaca cagctcttgg
     8221 tggtacttat gacattttaa ctggtgcagc taaaggtact caagtattcg gtcaagaccc
     8281 actatggctt gcatgggtaa cagaccttgt aaaccttaaa ggtactgata ctagtcaata
     8341 tcaacacttg ttagatacag tacatccagc tcgtttcaaa gttggacaaa tgatcggttc
     8401 attcggtatc ttgatgggtg tgattgttgc tatctaccgt aatgttgatg ctgacaagaa
     8461 acataaatac aaaggtatga tgattgcaac agctcttgca acattcttga caggggttac
     8521 tgaaccaatc gaatacatgt tcatgttcat cgcaacacct atgtatcttg tttactcact
     8581 tgttcaaggt gctgccttcg ctatggctga cgtcgtaaac ctacgtatgc actcattcgg
     8641 ttcaatcgag ttcttgactc gtacacctat tgcaatcagt gctggtattg gtatggatat
     8701 cgttaacttc gtttgggtaa ctgttctctt tgctgtaatc atgtacttta tcgcaaactt
     8761 catgattcaa aaattcaact acgcaactcc agggcgcaac ggaaactacg aaactgctga
     8821 aggttcagaa gaaaccagca gcgaagtgaa agttgcagca ggctctcaag ctgtaaacat
     8881 tatcaacctt cttggtggac gtgtaaacat cgttgatgtt gatgcatgta tgactcgtct
     8941 tcgtgtaact gttaaagatg cagataaagt aggaaatgca gagcaatgga aagcagaagg
     9001 agctatgggt cttgtgatga aaggacaagg ggttcaagct atctacggtc caaaagctga
     9061 cattttgaaa tctgatatcc aagatatcct tgattcaggt gaaatcattc ctgaaactct
     9121 tccaagccaa atgactgaag cacaacaaaa cactgttcac ttcaaaaatc ttactgagga
     9181 agtttactca gtagcagacg gtcaagttgt tggtttggaa caagtaaagg atccagtatt
     9241 tgctcaaaaa atgatgggtg atggatttgc agtagaacct gcaaatggaa acattgtatc
     9301 tccagtttca ggtactgtgt caagcatctt cccaacaaaa catgcttttg gtattgtgac
     9361 ggaagcaggt cttgaagtat tggttcacat tggtttggac acagtaagtc ttgaaggtaa
     9421 accatttaca gttcatgttg ctgaaggaca aaaagttgca gcaggagatc tccttgtcac
     9481 agctgacttg gatgctatcc gtgcagcagg acgtgaaact tcaacagtag ttgtcttcac
     9541 aaatggtgat gcaattaaat cagttaaatt agaaaaaaca ggttctcttg cagctaaaac
     9601 agcagttgct aaagtagaat tgtaatatac ttgaggttgg aagctgtatt ccaacctctt
     9661 attttgggag aaaagaatga aatttttaac actcaatact cacagttgga tggagaaaga
     9721 agcagaggaa aaattccaga ttttgcttga agatattctt gaaaaggact atgatttgat
     9781 ttgtttccaa gaaatcaatc aggagatgac ctcgtcagag gtggaggtta atgaccttta
     9841 tcaagctttg ccagcagctg agcctattca ccaagaccat tatgttagac tcttggttga
     9901 aaagttgtta gagcaaggga aaaattacta ctggacctgg gcttataacc atatcggcta
     9961 tgaccgctac cacgaaggtg tggctatctt gtctaaaaca cctattgaag ccagagaaat
    10021 tttggtttca gatgtggatg atccaacaga ctatcatact cgccgtgttg ccctagctga
    10081 aactgtagtc gatggcaagg agctagcagt tgccagtgtt catctctctt ggtgggataa
    10141 aggttttcaa gaagaatggg cacgatttga ggctgtcttg aaaaaattga acaagccact
    10201 tttactagct ggagatttta acaacccagc aggtcaggaa ggttaccaag ctattttagc
    10261 tagtccatta ggcttacaag acgcatttga agttgctcaa gagaaaagtg gtagctatac
    10321 tgttccgcct gaaattgatg gctggaaagg gaacactgaa ccccttcgaa tcgattatgt
    10381 ctttactacc aaagagttag cggtggaaaa tttacatgtc gtatttgatg gtaacaagag
    10441 tccacaagtg agtgatcact atggcttgaa tgctatatta aactggaaat aataactgaa
    10501 aagaggttgg aactataaaa ttccagcctt ttcttactag agaagctact ggaaatagcc
    10561 taaataagtg agactactgt aatggaataa aatatggtat aattgataag atagatagaa
    10621 tcgaggatgt tatgtcattt acgaaatttc aatttaaaaa ctatattaga gaagccttga
    10681 aggagttaaa atttacaact ccaacagagg tgcaagacaa gttgattcct attgttttgg
    10741 caggtcgtga cctagtagga gaatcaaaaa caggttcagg taagactcat actttcttgt
    10801 taccgatttt tcagcaatta gatgaagcta gcgatagtgt acaagcagtg attactgcac
    10861 cgagtcgtga gttggctact caaatttacc aagtagcgcg tcagatttca gctcactcag
    10921 atgtcgaagt tcgtgtggtt aattatgtgg gtggtacgga taaggctcgc cagattgaga
    10981 aattggcaag caatcagcct catattgtta ttggaacacc aggccgtatc tacgacttgg
    11041 ttaaatctgg tgatttagct attcataaag ccaagacatt tgttgttgat gaagcagata
    11101 tgaccttgga tatgggattc ttggaaactg ttgataagat tgctgacagt cttccaaaag
    11161 acttgcaatt catggtcttc tcagcgacta tcccacaaaa actgcaacca ttcttgaaaa
    11221 aatacttatc aaatcctgtt atggagaaaa ttaagaccaa aacggttatt tctgacacca
    11281 ttgataattg gttgatttcg accaagggac atgataagaa tgctcaaatt taccagttga
    11341 ctcagtcgat gcagccgtat ttggcaatga tttttgttaa cactaaaacg cgtgctgatg
    11401 aattgcattc atatctgact gctcaaggct tgaaggttgc aaaaatccat ggcgatattg
    11461 cccctcgtga acgcaagcga atcatgaatc aggtgcaaaa tctggatttt gagtatattg
    11521 tcgcaacaga tttggcagca cgtgggattg acattgaagg tgtcagccat gtcatcaatg
    11581 atgccattcc gcaagactta tctttttttg ttcaccgtgt tggtcgtact ggacgaaatg
    11641 gcctaccagg tacagctatt accctttatc agccaagtga tgactcggat atccgtgagt
    11701 tggagaaatt gggaatcaag tttagtccta agatggtcaa agacggggaa tttcaagata
    11761 cctatgaccg tgatcgtcgt gccaaccgtg agaaaaaaca agataaactt gatatcgaaa
    11821 tgattggttt ggttaaaaag aaaaagaaaa aagtcaaacc gggttataag aagaaaattc
    11881 aatgggcggt tgatgaaaag cgccgtaaaa ccaagcgtgc tgaaaatcgc gctcgcggtc
    11941 gtgcagagcg taaagctaaa cgccaaacat tttaatagaa attgttggag tactgagctc
    12001 caactttttt atttatgaga acgaactatc taaaccgaaa cactacatta aagactgcaa
    12061 attgcgatta aaaatggtat aatgataaag ttatatagtc ccgataagat ggtaggtatt
    12121 tattacgaag agttttccta tcagtagttt gcaattcgca aagaatgacg gcttcgaggt
    12181 cgctcgtctt tctacggttg ccgccatact gcggtcaccc tatgcgcctt actagcttca
    12241 gaaataaaaa agtatcgttt ttatttctga agctagtaac tcttagataa tggaaattcc
    12301 tatcagtact ttgtaactct ataacaatat tttttaaggg gggacatttt tatgtcagaa
    12361 cgtaaattat tcacgtctga atctgtatct gaggggcatc cggataagat tgcagaccaa
    12421 atttcagatg cgattttgga tgctatttta gcaaaggatc cagaggcgca cgttgctgct
    12481 gaaacagctg tatatactgg ttctgtccac gtttttggtg aaatttctac aaatgcctat
    12541 gtggatatta accgtgtggt tcgtgatacc attgcagaga ttggttatac caatacagaa
    12601 tatggatttt ctgctgagac ggtgggagta cacccatctt tggtggaaca atctcctgac
    12661 atcgctcaag gtgttaacga agccttggag gttcgtggaa atgctgatca agatccactg
    12721 gacttgattg gagcaggtga ccaagggctc atgtttggat ttgcagtaga tgaaacagaa
    12781 gagctcatgc cattgccaat tgcactcagt cataaattgg ttcgtcgtct ggcagaactt
    12841 cgtaagtctg gagaaattag ctatctccgt ccagatgcaa aatcacaagt tacagttgag
    12901 tacgatgaaa atgaccgtcc ggtacgtgta gatacagtcg ttatttctac tcagcatgat
    12961 ccagaggcca ctaatgaaca aatccatcaa gatgtgattg acaaggtcat caaagaagtt
    13021 attccatctt cttatcttga tgataaggca aaattcttta tcaatccgac aggtcgtttt
    13081 gtaatcggtg gtcctcaagg ggactcaggt ttgactggtc gtaagattat tgtagatact
    13141 tatggtggct actctcgtca tggtggtggt gccttctctg gtaaagatgc gactaaggtg
    13201 gatcgttcag cctcttatgc ggctcgctat attgccaaga atatcgttgc agcaggcctt
    13261 gctaagaagg cagaagtgca gttggcctat gctatcggtg ttgcgcaacc tgtttctgtt
    13321 cgtatcgata ctttcggtac aggaacagta gctgaaagtc aacttgaaaa agcggctcgt
    13381 caaatctttg accttcgccc tgcagggatt atccaaatgc tggacctcaa gcgtccaatt
    13441 taccgtcaaa catcggctta cggtcacatg ggacgtacag atattgatct tccatgggaa
    13501 cgtttggata aggtagatgc tttgaaagaa gcagtaaaat aagattttaa gaggggaacg
    13561 tcctctcttt tttatagttt ttaactatac tgggatactg ttctgaaaat ccattttgcg
    13621 aaagtagaga tttacatgta taatggattg aaactagaat agtacacctc aacttctaaa
    13681 acattgttag aaatcgattt gactgtcctg atcgatttct cctgttcttg tttcatttta
    13741 ctatatttct ttaaaaatga taaaggttga gatttctcct cgtaatagat aatcatgggg
    13801 atatttcaag ccaaagtttt attcgttatc acttgactat tgcaaggttt tctagatcaa
    13861 cagagtcatg gaatggactc atggttgaga tttctccttg ttgcttggac ttcattcaaa
    13921 agtctgttac ccaagccttg ttcaaacttc caatacacta gctgtttcca tagcatgact
    13981 tctgtactag actttctttt ccgaataaat agatagaacc acagaatcta gtaaacctag
    14041 aattaaaatt atggtataat attagcaata aaagaaatct ggaggattag aatcatggta
    14101 tcaacgaaaa cacaaattgc tggttttgag tttgacaatt gcttgatgaa tgcagcaggt
    14161 gtggcttgta tgacgataga ggagttagaa gaggtcaaaa actcagcggc aggaaccttt
    14221 gttactaaga cagcgacctt ggacttccgt caggggaatc ctgagccacg ctaccaagat
    14281 gttccacttg gttccatcaa ctctatgggc ttgccaaata atggcttaga ctattatttg
    14341 gattatcttt tagatttgca ggaaaaagag tcgaaccgaa ctttcttctt atctctggtc
    14401 ggcatgtctc cagaggaaac ccatactatt ttgaaaaaag tccaagagag tgattttcgt
    14461 ggtctgactg agctaaatct ttcctgtcca aatgttccag gtaaacctca gattgcctat
    14521 gattttgaga caacagaccg gattttggca gaagtgtttg cttacttcac caaacctctt
    14581 ggaattaaat tgccacctta ttttgatatt gttcactttg atcaagcggc agctattttc
    14641 aacaaatatc cgctcaagtt tgtcaactgc gttaactcta tcggaaacgg cctctatata
    14701 gaagacgaat ctgtcgttat tcggcctaag aatggttttg gtggaattgg tggagaatac
    14761 atcaaaccga ctgctctagc caatgttcac gccttttatc aacgtttaaa tcctcaaatc
    14821 caaattatcg gaacaggtgg cgttctgact ggtcgagatg cctttgaaca catcctctgt
    14881 ggagcaagta tggtgcaggt gggaacgacc cttcacaaag aaggcgtcag tgcttttgac
    14941 cgcattacca atgaactgaa agcaatcatg gtggaaaaag gctacgagag cttagaagat
    15001 ttccgtggga aattgcgcta tattgactaa attaaatcaa aaaatctgaa gaaaggagag
    15061 acgatgctag ccattgaaga aagtcagaag ttgactttat caaatttacc gagcctgagc
    15121 ctatttacag ggacagatca gggtcagttt gaagtgatga agagtcaaat gttgaaacag
    15181 attgggtatg attctgctga cctcaacttt gcctactttg atatgaaaga agtagtttac
    15241 aaggatgtgg aactggagtt ggtcagcctt cctttctttg cggatgagaa aatcgtgata
    15301 ttagattatt ttatggatat cacgactgct aagaaacgct ttttgacaga tgatgagctt
    15361 aagtcatttg aggaatacct tgacaatcct tctccaacaa ccaagttgat aatctttgca
    15421 gaaggaaagc tggatagcaa aagacggtta gtcaaattac ttaagcgtga tgccaaggtc
    15481 ttcgatgcag tagaagtaaa agaacaagaa ttgcgccagt atttccaaaa gtggagtcag
    15541 aaacaaggtc tgcagtttac caatcattct tttgaaaatc tcctcatcaa gtcggggttt
    15601 caatttagcg aaatccagaa aaatcttctc tttttacagt cctataaggc gaattctgtt
    15661 attgaggaag aggatattgt taacgcaatt cccaagacct tgcaggacaa tatttttgat
    15721 ttaactcagt ttattctgac taaaaagatg gatcaggcgc gcgatttggt gagagacttg
    15781 accttgcaag gggaagatga aatcaaactg attgcagtca tgctgggaca atttcggact
    15841 tttactcagg tgaagatttt ggcggagtct ggccaaacag aatcgcagat tgcaagtagt
    15901 ttaggtagtt atctgggacg taacccaaat ccttatcaaa tcaagtttgc attaagagat
    15961 tcgagaggac tttctttgag ctttctgaag caagctattt cctatttgat tgagacagac
    16021 tatcagatta agacaggtct ttatgaaaaa ggtttccttt ttgaaaaggc actcttacag
    16081 attgctagtc aggtcaattg acatttgttg aaactacaac ccgcgtcagg attatcttgt
    16141 cgtgggtttc ttgctgattt gcttatttta ttagaaagta tgtcagtgat ttccttatct
    16201 ataagcaaaa aacttgaaaa aagtgaatgt ttgcgttatc attttcatat agaaagaaaa
    16261 agaggtatta cagatggcta ttatcttacc agaacttcca tatgcatacg acgctttgga
    16321 accatacatc gatgcggaaa caatgcattt gcaccatgac aaacaccatc aaacttatgt
    16381 caacaatgcc aatgcagctt tagaaaaaca ccctgaaatc ggtgaagacc ttgaagcctt
    16441 gcttgctgat gtagaatcta tcccagctga tatccgtcaa gcacttatca acaatggtgg
    16501 cggacacttg aaccacgctc ttttctggga attgatgact cccgagaaaa cagctccttc
    16561 agcagaactg gcagcagcaa tcgatgcaac atttggttca tttgaagaat tccaagcagc
    16621 cttcactgca gcagcaacaa ctcgttttgg ttcaggttgg gcatggttgg ttgtcaacaa
    16681 agaagggaaa cttgaagtga cttcaacagc aaaccaagac acaccaatct cagaaggtaa
    16741 aaaaccaatc ttgggcttgg acgtttggga acatgcttac tacgtgaaat accgcaacgt
    16801 gcgtcctgac tacatcaaag ctttcttttc agtaatcaac tggaataaag tagatgaatt
    16861 gtacgcagct gctaaataat gatagttgga gggaagaatt gttcttctct ttttaggtta
    16921 taagattctg gtctgacaaa atcgtcagac ttttttcatt tttatgagaa acgtgctaac
    16981 tgctagaaat tatggtagaa taaagtatta agtaatcgtg gaaaaaggat atctatgcga
    17041 aaagaaattg cacctgaatt atacaactat aacaagtttc ctggtccgga gttccattta
    17101 cacggggata aggtcgaaac ggaagggata gctttttcct tggttgaaaa tatcaaggat
    17161 gcctttgatg tgacgacttt taatcagcgt ttttcagaag tcttaaccaa gtttgattat
    17221 atcgtggggg actggagcaa cgaacagctt cgcctacgag gtttttacaa ggatgaccga
    17281 acagaagaaa aacttgaaaa aatcagtcgt ttacaagact accttttaga gtattgtagt
    17341 tatggttgtg cctattttgt cttagaaaat gaagccccta agcgagcatc atttgacaag
    17401 aaaatgcgta agacggaaga agaaacacct tctagaaaag gaaagaaacc gactcaaact
    17461 aaacgaaagt cgaatgcaga taagaaaaat agacgtcgtc agaaagacca gcattctcag
    17521 aaagaggaca aggaacaacg tcattttgtc attcgtcaga agtgagtaga gaataaggag
    17581 aagaaatgaa accctcaatt catagtttga cacatcaaac catgcaggaa tgggtattgg
    17641 agcagggaga aaagaaattc cgtgcagatc aaatctggga atggctctac cgtaaacgtg
    17701 tgcagtcatt tgaagaaatg accaaccttt ccaaggattt gattgctaag ctcaatgacc
    17761 agtttgtggt caatcccttg aaacaaggta ttgtgcaaga gtctgctgat ggtacagtca
    17821 aatatctctt tgaattgcct gacggtatgt taattgagac tgtactcatg tgtcaacact
    17881 atggtttatc tgtctgtgtg accactcagg tcggctgtaa tatcggttgt accttctgtg
    17941 cctctggttt gattaagaag cagcgtgacc tcaataacgg tgaaatcgta gcgcaaatca
    18001 tgctggttca gaaatacttt gatgagcgtg gtcaggatga acgcatcagc catatcgttg
    18061 tcatgggaat cggtgagccc tttgataatt acaacaatgt cttgaatttc tttcgtacta
    18121 tcaatgatga taaaggaatg gctatcggtg ctcgtcacat cacggtttca atctcaggtt
    18181 tggctcataa aattcgtgac tttgctgatg aaggggttca ggtcaatctt gccgtgtctc
    18241 ttcacgcacc caacaatgaa cttcgttcaa gtatcatgaa gattaaccgt gcctttccga
    18301 ttgaaaaact ctttgctgct attgaatact acattgagac aacaaaccgt cgtgtaacct
    18361 ttgaatatat catgcttaat gaagtcaacg acggtgtgga acaagccttg gaattggctg
    18421 aattgctcaa gaacatcaag aaatcgtctt atgtaaactt gattccttat aacccagtta
    18481 gtgagcatga ccaatatagc cgtagtccca aagagcgcgt gttggctttc tatgatacgc
    18541 ttaagaaaaa agggggcaac tgtgtggttc gtcaagagca tggtacagat attgacgcag
    18601 cttgtggaca attgctcttt aacacaatga aacgtgaccg ccagaaggca gtcgcagcgg
    18661 taaatcctta atgatgaaga agacttataa tcatattttg gtctggggag tcattttcta
    18721 tagcatttgc attgtctatt tttgctttac tcctcaagaa caatctaccg tgggagtggg
    18781 aactccagat attcagcatc ttggacgcct ggtttttctt ttgactcctt tcaattctct
    18841 ctggaaactg ggcgaagtga gtgacattgg acaattatgt tggatttttt tacaaaatat
    18901 cctcaatgtc ttcttgtttt ttcctctgat tttccaactc ctttatctat ttccaaattt
    18961 gcggaaaaca aaaaaggtcc ttctttttag ttttcttgtg agtcttggaa tcgagtgtac
    19021 gcaattaatc ttggactttt tctttgattt caatcgcgtc tttgagattg atgatttgtg
    19081 gaccaacact ttgggtggct atctggcttg gctcctttat aaacgattac ataaaaacaa
    19141 gataaggaat taaaatgagt attttagaag ttaaaaatct gagtcacggt tttggtgacc
    19201 gtgcaatttt tgaagatgtg tccttccgtc tcctcaaggg ggaacatatc ggcctggtcg
    19261 gtgccaatgg tgaaggaaaa tcaaccttta tgagtatcgt gactggtaaa atgctgccag
    19321 atgaaggaaa ggttgagtgg tccaaatatg tgacggctgg ttacttggat cagcactctg
    19381 tccttgctga aagacagtcg gtgcgtgatg ttctccgtac ggcttttgat gagcttttca
    19441 aagctgaagc tcgtatcaat gacctctata tggaaatggc tgaagacggt gcggatgttg
    19501 atgctctcat ggaagaagta ggagaacttc aagaccgtct ggagagtcgt gatttctata
    19561 ccttggatgc taagattgac gaagtagcgc gtgctcttgg tgttatggac tttggcatgg
    19621 atacggatgt aacttctttg tcaggtgggc aaagaaccaa ggtgcttttg gcaaaacttc
    19681 tccttgaaaa gcctgatatc ttgctgttgg acgagccgac caactacttg gatgctgagc
    19741 atattgattg gctcaagcgc tatctccaaa actatgagaa tgcctttgtt ctcatttcgc
    19801 acgatattcc attcctcaat gacgttatca atattgtcta tcatgtggaa aatcaacagc
    19861 tgacgcgtta ctctggtgac tactaccagt tccaagaagt ttatgctatg aagaaatctc
    19921 agctagaggc agcctacgaa cgccagcaga aagagattgc agacctcaag gactttgtgg
    19981 ctcgtaataa agcccgtgtt gcaactcgta atatggctat gtctcgtcaa aagaaattgg
    20041 ataagatgga tattatcgaa ctccaaagtg agaaaccaaa accatccttt gatttcaaac
    20101 cagctcgtac accagggcgc tttatcttcc aaaccaagaa cttgcaaatt ggttacgacc
    20161 ttcctcttac taagccttta aatcttacct tcgaacgcaa tcaaaaggtt gcgattattg
    20221 gtgctaatgg tattggaaaa acaactctct tgaagagtct cttgggcatt atctcgccaa
    20281 tcgctgggga agtggagcgt ggagattatt tagaacttgg ttattttgag caggaagtag
    20341 aaggcggtaa tcgccaaact cctcttgaag ctgtctggaa tgcctttcct gcccttaatc
    20401 aagcagaagt ccgtgcagcc cttgcccgtt gtggtttgac aaccaaacat attgaaagcc
    20461 agattcaagt attatcaggg ggagagcaag ccaaggttcg tttctgtctc ttgatgaatc
    20521 gtgaaaacaa cgttttagtg ctggacgagc cgaccaacca tttggatgtg gatgcaaagg
    20581 atgagctcaa acgcgctctc aaagaatata ggggatctat ccttatggtc tgccacgagc
    20641 cagactttta tgaaggctgg atagaccaaa tatgggattt taataattta acttaagaat
    20701 gagagtaaaa gaaatacagt accgaatgtg gatactgtat ttttttggtt tttaagattt
    20761 aaaatcgtag tctttcacca cttcgatgct gtcgattgtg atagcagtag ttggcttgtc
    20821 tttttcatct ttttcggcct tagcaatctt atccacaaca tccataccgt caatcacttg
    20881 accaaagact gggtgtttgc catctagact agggtttcca ccttctttgt aggcttcgat
    20941 aattttctgt ggatacttgc ttgtagggag tttagaagag gtatctgtag agctttggtt
    21001 gatgaagaac tggctgccat tggtgtttgg ttgaccagta ttagccatag caagagcacc
    21061 acggatgtta tacaaataag gagtaatctc gttcttgaaa ccagttcctt tgtctttagt
    21121 cttatccttg tcatgccaga tggactgacc acctgtaccg tccccttttg gatctccagt
    21181 ttggaccata aagccatcga tgacacggtg gaaggtaata ccgttatagt agccttcttt
    21241 ggcgtgagtg aggaaatttt caaccgctag aggagcgagt ttagggaaga gtttaatgcg
    21301 aatatcacct tggcttgtgt ggagaatcac ttcggcttca tcttcagcaa cttccttaga
    21361 tagttgtggg aaattggcgt tttcgtttgt taaagcatcg tttaactcct tggcagattg
    21421 ggcagctact ttggaacttt cttcagcagc aagactggaa tcaacataat catcaccacg
    21481 cagactgcgt tggacgctgc tacacccagc tagggctaca gtagacagta aaagaagggt
    21541 tgctagtttt ttcattctga tcctctcaaa aattcattta taccattgta ccctaaaaag
    21601 aagtggattt caaatattag tagtttacag aactggctta gataatagtt gggatttcat
    21661 catcatcttg aaaatcttca acttcttcaa cttcttcccc agtaaatggt ttctgaaaag
    21721 aacttagtat aaaacgggca attggttcaa caattaggaa atttagagga agagctgcaa
    21781 taaagtttac gaaccaggcg cttagatagg tattgggtgt gatagcaatg ccattgtaaa
    21841 aaagtccata gaaggacatg aaagtgacca gctaattctt cttctgaaaa atgctcatga
    21901 agggcttgat ccaggtcgca agatttcatt ttagaaagtc cagtaactcc cttcttcttc
    21961 aggatattct ttttcatggt attatttaga tggtcaagtg agtcaaaaga agtttcgaca
    22021 agagcatacc ctttttctat cagagttttt agatgtttag gggcatcaat accataagtg
    22081 tactcaaagt attttggaaa ccagatctta agaaagctcg taaagctagc cagttctcta
    22141 aacgctaaga actagctaca acacagcatt tacaacactt catggttcac accatggggt
    22201 gctttttaat gattttaagc aaaattcaca cttaattgaa agttatttaa attagatgaa
    22261 aatttatttt ttgaaaaagc gggaaaacat tgattctaag cgaattttga atcatattcg
    22321 aatgggagtt tttaaaatca tgtttcagtg gtaaaagtac caaactggat tctccatagt
    22381 aaaatgatat ctcctgctag tgaatcatct actaataagt ccatatttcg cttgggaacg
    22441 ggcttgggac ttagaagcca agtatccaac tctcgttcag gcgatatgaa gggtttgact
    22501 tggtgttctt tataaaattt ttctaaaata gtattcacgg atagcatctt tcaaacattt
    22561 atttcctatt ctaacaaatt tctaaatact ttaagaaatt tatagtctgt caagtttttt
    22621 tcttgacaca tgccagaaat ctgctataat agaacatgtg ctaaatagct cagctatttc
    22681 accgaaataa aaaattaaag aaatgagact ttaaaaatgg cagttaaaat ccgtttgact
    22741 cgtatgggtt ctaagaaaaa acctttctac cgtatcaacg tagcagattc acgttcacca
    22801 cgtgacggac gtttcatcga aacagttgga acttacaacc cacttgttgc tgaaaaccaa
    22861 gtaactttga aagaagaccg cgttcttgca tggttggcta atggagctca accttcagac
    22921 acagtacgta acatcctttc aaaagaaggc gtattgaaaa aattccacga ttctaaattc
    22981 tcaaaataag tttaaagtag gttgacagat ggatacgatt gaaaatctca ttattgcgat
    23041 tgtgaaaccc ttaatttcac aaccagatgc cttaactatc aagattgagg atacaccaga
    23101 atttttggaa tatcatttga atcttgatca aagcgatgtg ggtcgtgtaa tcggtcgtaa
    23161 gggtcgcact atttctgcga taagaacgat tgtctactct gtcccaactg aatacaaaaa
    23221 agtaagaatc gttattgacg aaaaataaga agggcgggac ggatgtctcg cttttttgtg
    23281 tggaatagaa g
//
LOCUS       DAVBNO010000029        22708 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVBNO010000029 DAVBNO010000000
VERSION     DAVBNO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22708
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.029"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(220..504)
                     /locus_tag="VQ846_001250"
     CDS             complement(220..504)
                     /locus_tag="VQ846_001250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075828.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDTTDEVSSKHCFEVVDRTDEVSNHTHGKPTLTWFEEIFEEYHSPFHN"
     gene            complement(666..953)
                     /locus_tag="VQ846_001251"
     CDS             complement(666..953)
                     /locus_tag="VQ846_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075829.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(963..1373)
                     /locus_tag="VQ846_001252"
     CDS             complement(963..1373)
                     /locus_tag="VQ846_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET3075830.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            1441..2172
                     /locus_tag="VQ846_001253"
     CDS             1441..2172
                     /locus_tag="VQ846_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075831.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            2169..3218
                     /locus_tag="VQ846_001254"
     CDS             2169..3218
                     /locus_tag="VQ846_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3075832.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVEVVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(3386..3715)
                     /locus_tag="VQ846_001255"
     CDS             complement(3386..3715)
                     /locus_tag="VQ846_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET3075833.1"
                     /translation="MTDTDPIKRAQTLITDLNKAYQACKQATADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            3992..4330
                     /locus_tag="VQ846_001256"
     CDS             3992..4330
                     /locus_tag="VQ846_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3075834.1"
                     /translation="MKHMIIQTQKTVYKVNVDDVYYIQTHPTKAHTVKIVTEEASFNM
                     VQNLSNLENQYGETLMRCHRNCLVNLDKVKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            4335..5072
                     /locus_tag="VQ846_001257"
     CDS             4335..5072
                     /locus_tag="VQ846_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3075835.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITLSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            5086..6426
                     /locus_tag="VQ846_001258"
     CDS             5086..6426
                     /locus_tag="VQ846_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET3075836.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNVYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNVRDRALKSLLAGKFIKARDKNIVFNVEVPEEI
                     QVEGVSLLDFLTVVSILCDNAIEASVEACQPHVSIAFFKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(6470..6625)
                     /gene="blpC"
                     /locus_tag="VQ846_001259"
     CDS             complement(6470..6625)
                     /gene="blpC"
                     /locus_tag="VQ846_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET3075837.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FYQPIQL"
     gene            complement(6682..8043)
                     /locus_tag="VQ846_001260"
     CDS             complement(6682..8043)
                     /locus_tag="VQ846_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET3075838.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPFSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSSEDEFGYHNTFMNFTKQSHDIELGISKTN
                     TEVSNQANLTNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQGQTQGTAEEPFLSQINQSIASLESSIASIKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            complement(8054..10207)
                     /gene="blpA"
                     /locus_tag="VQ846_001261"
     CDS             complement(8054..10207)
                     /gene="blpA"
                     /locus_tag="VQ846_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
                     /protein_id="HET3075839.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFSEWTGVAIFLAPKPSYQPHKDKKNGLLSFLPL
                     IFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVITYIL
                     QQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDANSII
                     DALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLLSIPIYMFIIFSFMKPFEKMNH
                     DVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYSILQT
                     SLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQTKLQS
                     AKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDINLTIK
                     QGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINYQDIKNIDKKVLRRHINYLPQQ
                     AYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAGLSGG
                     QKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLISLTDKTILFVAHRLSIAE
                     RTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            10489..10743
                     /gene="blpM"
                     /locus_tag="VQ846_001262"
     CDS             10489..10743
                     /gene="blpM"
                     /locus_tag="VQ846_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HET3075840.1"
                     /translation="MNTKMMEQFHEMDIAMLSSIEGGKNNWQTNVFEGGSAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVALWAGVTGATGGF"
     gene            10759..10962
                     /gene="blpN"
                     /locus_tag="VQ846_001263"
     CDS             10759..10962
                     /gene="blpN"
                     /locus_tag="VQ846_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HET3075841.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            11206..11355
                     /gene="blpO"
                     /locus_tag="VQ846_001264"
     CDS             11206..11355
                     /gene="blpO"
                     /locus_tag="VQ846_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HET3075842.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            11459..11578
                     /locus_tag="VQ846_001265"
     CDS             11459..11578
                     /locus_tag="VQ846_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET3075843.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            12046..12417
                     /locus_tag="VQ846_001266"
     CDS             12046..12417
                     /locus_tag="VQ846_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075844.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLASIL
                     VTVYLTREMRIKEFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQIIAYI
                     GAILCLTIMLLLIWGLKYYKK"
     gene            13046..13429
                     /locus_tag="VQ846_001267"
     CDS             13046..13429
                     /locus_tag="VQ846_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET3075845.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            13481..14170
                     /locus_tag="VQ846_001268"
     CDS             13481..14170
                     /locus_tag="VQ846_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET3075846.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            14212..14445
                     /locus_tag="VQ846_001269"
     CDS             14212..14445
                     /locus_tag="VQ846_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075847.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            14596..15207
                     /locus_tag="VQ846_001270"
     CDS             14596..15207
                     /locus_tag="VQ846_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET3075848.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGVFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            15368..16162
                     /locus_tag="VQ846_001271"
     CDS             15368..16162
                     /locus_tag="VQ846_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET3075849.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            16159..16794
                     /gene="trmB"
                     /locus_tag="VQ846_001272"
     CDS             16159..16794
                     /gene="trmB"
                     /locus_tag="VQ846_001272"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET3075850.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            16920..17399
                     /gene="rimP"
                     /locus_tag="VQ846_001273"
     CDS             16920..17399
                     /gene="rimP"
                     /locus_tag="VQ846_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET3075851.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            17443..18579
                     /gene="nusA"
                     /locus_tag="VQ846_001274"
     CDS             17443..18579
                     /gene="nusA"
                     /locus_tag="VQ846_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET3075852.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            18601..18894
                     /locus_tag="VQ846_001275"
     CDS             18601..18894
                     /locus_tag="VQ846_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET3075853.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            18887..19186
                     /locus_tag="VQ846_001276"
     CDS             18887..19186
                     /locus_tag="VQ846_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET3075854.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            19203..21995
                     /gene="infB"
                     /locus_tag="VQ846_001277"
     CDS             19203..21995
                     /gene="infB"
                     /locus_tag="VQ846_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET3075855.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQVAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDCYNDI
                     KMDDVIEAYVMEEIKR"
     gene            22246..22596
                     /gene="rbfA"
                     /locus_tag="VQ846_001278"
     CDS             22246..22596
                     /gene="rbfA"
                     /locus_tag="VQ846_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET3075856.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         6696 a         3958 c         4959 g         7095 t
ORIGIN      
        1 gtttcctagt ttgctttttg attttcattg agtataaaca aaaagagctg tcgggctctt
       61 tttactttat agatttttta agactttcct aagtaatgac ggacggtagc gacctccttc
      121 gaagttccat acctaaactt tgaacctaag tcttaaagtt tccggacagc tgaaaccaag
      181 ctgtttcagg tgttttcatt acggcagaaa gtcttcgatt tagttgtgaa atggtgaatg
      241 atactcttca aaaatttctt caaaccacgt cagcgtcggc ttgccatggg tatggttact
      301 gacttcgtca gttctatcca caacctcaaa acagtgtttt gagctgactt cgtcagtcgt
      361 atctacaacc tcaaaacagt gttttgagct gacttcgtca gttctatcca caaccttaaa
      421 acggtgtttt gagcagtctg tgcctagctt tctagtttgc tttttgattt ttattgagta
      481 tgagttacct aaattatgat gcatagttga tgggatatat ataatagatt gaaatagaat
      541 atgaacaaat tgataagagg attttaaagt aatctctaac aatgctttag aaactatagt
      601 gtgctattct aaattcaatt cactataact tgtttacgtt ttaaaaaaga gccggggggc
      661 tctttttact tatcttcagt tccctgcatt tcttttatca cagctagtct agtctggata
      721 tccttttcca agaccttaaa cttgtaagtc aagtcttctt ggtattcctt gataagttct
      781 ttttgctggt taatgatttg caggctgttt tggataatat ccacatcgtc cttgatagct
      841 tgaacgcggt cagtggtatt caagacttca tctgtgatgg tttggcgatt ttttgtaacc
      901 agataacttc cggctgcagc tcctgcaaat agcagtaggt tggataattt catagcaact
      961 ccttaagcgt ttttgatggt ttcagcgact tgagcaagtt tgtcaaagtc tggttcgtgg
     1021 gcgataaaat caatcttgag gtcatcgtca gcactgtagc gaggcacaag gtgaacgtga
     1081 gtatgaaaaa ctgtttgacc agcgacttct tcacagttgg aaatgatatt cataccagca
     1141 gccttagtga ctttcatgac tttttgagct acttttggta cttgggcaaa gagttggctg
     1201 gcgctcgtag catccatctc caaaagattg cgatagtgtt cttttggcac gaccaaggtg
     1261 tgtcctagtg ttacttgaga gatatcaaga aaggcaagga cctgctcatc ttcatatact
     1321 tttgaagcag gaatttcccc tgcgatgatt ttacaaaaaa tgcaatctga cataaaatct
     1381 acctctactg tactgaattt tgatataata tagctacatt ataccagatt tggagaaaat
     1441 atgttagaaa ttaaaaacct gacaggtggc tatgttcatg ttcctgtttt gaaagatgtg
     1501 tcctttactg ttgaaagtgg gcagttggtc ggtttgattg gtctcaatgg tgctgggaaa
     1561 tcaacgacga tcaatgagat tatcggtctg ttggcacctt atagtggctc catcaatatc
     1621 aatggcctga ctctgcaagg agatgcgact agctaccgca agcagattgg ctacattcct
     1681 gagacgccta gtctgtatga ggaattgacc ctcagagagc atatcgaaac ggttgctatg
     1741 gcttacggta ttgagcaaaa agtggctttc gaacgagtag agcccttgtt aaaaatgttc
     1801 cgtttggaac agaaattaga ctggttccct gttcattttt caaaagggat gaagcagaag
     1861 gtcatgatta tctgtgcttt tgtggtggat ccaagtcttt tcatcgtgga tgagcctttc
     1921 cttggtcttg atccgctggc tatttctgat ttgattcagc ttttggaagt ggagaagcaa
     1981 aagggcaagt ctattctcat gagtacccac gtgctggatt cggcggagaa gatgtgtgat
     2041 gcctttgtca ttcttcacaa gggagaggtg cgtgccaaag gcaatctcct gcaactacgt
     2101 gaagcctttg atatgcctga ggctagtttg aatgatattt acttggctct gaccaaagag
     2161 gaggatctat gaaagacttg tttttaaaga gaaagcaggc ctttcgtaag gagtgtcttg
     2221 gttatctgcg ctatgtgctc aatgaccact ttgtcttgtt cctgcttgtc ctgttgggct
     2281 ttctagccta ccagtacagt caactcttac aacattttcc tgaaaatcat tggcctatcc
     2341 ttttgtttgt aggaattacg tctgttttac ttttactttg gggaggaatt gccacctata
     2401 tggaggctcc agacaagctc tttctcttag ttggagaaga ggaaatcaag ctccatctca
     2461 agcgtcaaac tggcatttcc ctagtctttt ggctctttgt acagaccctt ttcttgctgt
     2521 tatttgcgcc cttgttttta gcaatgggtt atggcttgcc agtttttctg ctctatgtgc
     2581 ttttattggg ggtaggaaaa tatttccact ttcgtcaaaa ggccagcaaa tttttcactg
     2641 aaactggact ggactgggac tatcttattt ctcaagaaag caagcgtaag caagtcttgc
     2701 ttcgtttctt tgccctcttt acgcaggtca agggaatttc aaacagtgtc aagcgtcgtg
     2761 cctatctgga ctttatctta aaggctgttc agaaggtgcc tgggaagatt tggcaaaatc
     2821 tctatctgcg ttcttatctg cgaaatggcg acctctttgc tctcagtctt cgtcttctct
     2881 tactttccgt actggcgcag gtttttatcg agcaagcttg gattgcgaca gcagtggtag
     2941 ttctctttaa ctacctcttg ctcttccagt tgctggccct ctatcatgcc tttgactacc
     3001 agtatttgac ccaactcttt ccgctggaca aggggcaaaa ggaaaaaggc ttacaggagg
     3061 tagttcgagg attgaccagt tttgttttac ttgtggaagt agttgttggg ttgattacct
     3121 tccaagaaaa actagccctt ctagccttac taggagctgg tttggtttta ctagtcttgt
     3181 atttgcctta tcaggtaaaa cgtcagatgc aggactaaca ttgctgatac gacactaaaa
     3241 aagaagttga gttcagtctg tctcaacttc ttttttgtta ctacaggata atggttggtc
     3301 cgtagagact tatactcttc gaaaatctct tcaaaccacg tcagcgtcgt cttaccgtac
     3361 tcaagtacag cttgcggcta gcttcctagt ttgctctttg attctcattg agtattaact
     3421 tggtcttgac ttggtcaaag tggaagcggt cataggcccg ccaagcggcg cgagttggag
     3481 catctggatc aagagcgctg agtcccatga gaagactgga agtctggtaa aatttttcca
     3541 attcaatcaa gaatcgatta tccactgttt cagccttggc tagaaaacca agaatagagt
     3601 ttaattgctc ctgaaagcgg acgtcgtcag cggttgcctg tttgcatgct tgataggctt
     3661 tgtttaagtc agtaatcaaa gtctgagctc ttttgatagg gtctgtatct gtcatgagaa
     3721 ttcctcctct aatctgggtg ccagtcttga ttctagcaac tgtgttttga tactgtcagt
     3781 ctatcacttt tatctccttt ttcgccttta aatctttaat tgtcattgaa atgtcttgaa
     3841 ttgcgctgag tgaattttat gataaaatag tggtaagctc atcatgatat tgtagaaaaa
     3901 taattctttt aggagctttc aaagactgtt taggattggg tgtgcttgtg ttagattttt
     3961 tctgttattc ttttcttagg aggagaatcc aatgaaacat atgattattc agacacagaa
     4021 aacagtctat aaagtaaacg tcgacgatgt ctactatatc caaacacatc caactaaagc
     4081 ccatactgta aagattgtta cagaagaggc tagttttaat atggttcaaa atttaagtaa
     4141 tcttgagaac caatatgggg aaaccttgat gagatgtcat cgaaattgtt tggtcaatct
     4201 tgataaagta aaatcgattg attttcaaga aagaatcctt tttcttggag aagaaggtca
     4261 atacgctgtc aagtatgcca gacgtcgcta tagagaaatt cgtcaaaaat ggttgaaaga
     4321 aggagagtaa gaagatgaga atatttgttt tagaagatga tttttcccaa cagactagaa
     4381 ttgaaacgac gattgagaaa ctcttgaagg aacatcatat cactctgagt tcttttgaag
     4441 tttttggtaa gccagatcaa ttgctggcag aggtgcatga gaagggggcc catcagctat
     4501 tctttttgga tattgagatt cgaaatgagg agatgaaggg tctggaagtg gctagaaaga
     4561 ttcgggatcg ggatccgtat gccctgattg tctttgtgac gactcactcg gagtttatgc
     4621 ccctgtcctt tcgctaccaa gtgtctgctc tggattatat tgataaggcc ctttcggcag
     4681 aggagtttga atctcgtatc gagacagccc tcctctatgc caatggtcaa gacagtaaaa
     4741 gtctagcgga agattgcttt tactttaaat caaaatttgc tcaattccag tatcctttta
     4801 aagaggttta ctatctcgaa acgtccacaa gaccccatcg tgttattctc tataccaaga
     4861 cagacaggct ggaatttaca gcgagtctag aggaggttct caagcaggag cctcgtctct
     4921 tgcagtgcca tcgctctttt ctcatcaatc ctgccaatgt ggtgcattta gataagaaag
     4981 aaaaactgct tttctttccc aatggtggaa gttgtctaat cgcgcgttat aaggtcaggg
     5041 aagtgtctga ggctattaat aacttacact gagctaggag agtttatgaa tgttgcttgg
     5101 atattgttgt atacacttgt tactcacgga ttaaaaattg tcatattctt taaggtagat
     5161 ggaattagtc tcacttttga gaggattttt aaggcctttc tttttaagat actgttggcc
     5221 gttgtttttg gaatgcttgg ctatatggta ggaaatgttt acctatctta ttttatggaa
     5281 cccttgtacg gcataggctt atctttctta ttgttaagag aacttcctaa aaaactcctt
     5341 ctcttttatg gtctctttcc aatgatatta gtgaatctct tttatagagg tgtttcctat
     5401 tttgtgcttc catttttggg acaagggcaa gtatatgatg actactcatt tatttggtta
     5461 tgtataataa ttttcaattt cttcatttct ctagcttttt tgaaatggtt ggactatgat
     5521 ttcactagct tgagaaaggg gattctcgat aaagattttc aaaagtccct gactcagatt
     5581 aactggataa tgggggctta ctatttggtg atacaaaatc tgtcttactt tgaatatgaa
     5641 caggggattc aatcaacgac tgttcgccat ctcatcctag tcttttacct gctctttttt
     5701 atggggatta tcaagaaatt ggatacctat ttgaaggaca aactccatga gagactgaac
     5761 caagagcagg acttgcgcta cagagagatg gagcggtata gtcggcatat agaggagctt
     5821 tataaggaag tacggagttt tcgccatgac tacaccaacc tcttgactag cttacgtctg
     5881 ggcattgaag aggaggatat ggagcagata aaagagatct acgactcggt cttaaaggat
     5941 tctagtgaaa aattgcagga caataaatat gaccttggcc gattggtgaa tgttcgggat
     6001 cgtgccctca aaagtctcct agctggaaaa tttataaaag ctagagataa gaacattgtc
     6061 tttaatgtcg aagttccaga ggagattcag gtagaggggg tgagtctact tgattttcta
     6121 accgttgtgt ctatcctttg tgacaacgct attgaagcta gtgtagaagc ctgtcaacct
     6181 catgtttcaa ttgccttttt taaaaatgga gcacaggaga cctttatcat tgaaaattcc
     6241 atcaaagaag agggaatcga tatttctgaa atcttctcct ttggagcaag ttctaaaggg
     6301 gaggagagag gagttggtct ctataccgtt atgaaaattg tggaaagtca tcccaatacc
     6361 agtctcaata ccacctgcca agatcacgtt tttcgtcagg tacttactgt aatacatata
     6421 gaatgaagaa aaaataagac cgagagtgcg ttctcttggt cttgtttttt tatagttgta
     6481 ttggctgata aaatcctttt gtattttttt gttcaattat attaaatcta tataagaaat
     6541 cttcccacca tcctccacct ataatttggt tgagttcggt agttgttagt tcttgaaatg
     6601 aagttaggtt ttgtttctta tccatgttat gattctcctt tttgataaga taataaatag
     6661 ttatagagtg ttatctgaaa attaatcaga atgggttaaa attttatctt tgaaataatc
     6721 aaaatatgtt ttctttgtag ttacactagt gacgcgacct tgtaagccat attggatgag
     6781 tttactatcc tcgttagata gttttgcaag agcggttaat ttaaagagat tgccttgctc
     6841 tgttctggta ggagtttgat caatactctg aagttggccg atgatggtgg tgccgtgatt
     6901 tccaatcttc tccagtttta atcttacagt ttgtccttta tctagtaagg ggagatagtc
     6961 agatgatacg tagtaagtga ttagtacttc tcttgtatct gtgatgacag ggaatatttg
     7021 agcaatttct gtaccagttg gaattctatt tttaccttca aattcgctgt tcagatgaac
     7081 gataccttta cttggagcgg ttaaggtatt attttctaag cgctgtgtcg cttgatctag
     7141 ttgtactttt aattctgtta attgattctc cacggttagt tgttgctgtg aggctgtttg
     7201 taaaaactga gtgcggagta cttcaatttt ggttgctaaa ctattatcat aagttgctac
     7261 acttccgata ccagcttgct gaattttgat gcttgcgata gatgattcaa gacttgcaat
     7321 actttgatta atttgagata aaaatggctc ctctgccgtt ccttgtgttt gtccttgaga
     7381 ggctataaga taacgattta aaattgactg gtgcggattg ccagttggta agcgtgctct
     7441 gttatttatg atagcatctc tcaactcttg atattctcca atttgttgtt gaacttttgt
     7501 aatttcttgt tcgatggctg atgaactgct attggtaaga ttagcttgat ttgaaacttc
     7561 agtgttagtc tttgagatac ccagttcaat atcatgggat tgtttagtaa aattcataaa
     7621 ggtattatgg tagccaaact catcctcgct agaaaaaaga tcagtcgctt tttctaagct
     7681 ttgtttcaag attccaagtc cttctttttg cttctcaagt ctttgtaatt gagttgctaa
     7741 ggcagttttc tgactttctt ccattgtttc agagtatttg atgagtaagt cacctttctc
     7801 aactacttga tttgccacta aatgattagc aaggatagga ttatcactgg tcgactgaat
     7861 agatgcaatg acacttgtag gggcgatttc tccttgggaa gtaacagtaa tttcttttgt
     7921 ggcaacaagg gagaaaatca agatgaaagt aaatagtaat gaaaaaggta taatgaacac
     7981 tgtcgcatag ttatggtaac gtctctgata aaactcgaca cttctaaaaa gattaggatt
     8041 catgacattc tccttattta ttgaatagat gatggtagaa gccttgcgcc tgcattaact
     8101 cttggtgact accaacttca atgattttcc cctggtcaag aacaatgaca cggttagttc
     8161 gttcggctat actgagacga tgggctacaa agagaatggt tttatcagtt agggatataa
     8221 gattatctat aacctttttc tcagtcaaga catcaagacc gctagtagct tcatctagta
     8281 ttaaaacagg agctttagtt aaaagagcac gagcgagagc gattcgttgc ttttgtcctc
     8341 ctgatagacc agctccatca gagagctgag tttgatagcc cataggcatt ctttcaatgt
     8401 cttgacggat ttcagctaat tcacaagctc ttagaatatc ttcttggcta atcatatgat
     8461 taccgcccaa ggttaaattt tccaagatag agccattaaa gatataggct tgttggggta
     8521 ggtaattaat atgacggcgc aagacttttt tatcaatgtt tttaatatcc tgataattga
     8581 tggaaatatg ccctttgtag ggttcaaaga aattgacaat cattttggct aaagttgttt
     8641 taccagaacc actaactcca actaggctaa ccttatctcc ttgtttaatc gtgagattaa
     8701 tatctgttaa ggtatctcgt ccaaaaccat acttataaga aaggtcatca aattcaatat
     8761 cgcccatcaa aaaatgtgaa tgaacagggt tttcttgaac ttgaaattca gattcgacta
     8821 gatagacttc gttcaaacgg ttattagcga ccttcgcaga ttggagtttg gtttggaggt
     8881 tgataatatt ttccatagga gttgtaaagt aagaaaaaag tgtgttaaag gtaatcagct
     8941 gaccgataga aattttactc gacatgacta attgagcgcc aaaccatagg ataaggatat
     9001 tcagaactaa ttttgttccc tgctttaaac tcgtttgtaa aatagaatat ttactgagct
     9061 taaaggattt ttccaaataa tctacaaatt cgctgtctat attttgatag cgattttctt
     9121 cactcgtgag cgactttata gtttcaatcc cgttgatatc ttcgataatg gcagagctaa
     9181 ccatagaatt actttgcatg acatcatggt tcattttttc gaaaggtttc ataaaagaaa
     9241 agatgatgaa catgtatata ggaatggaaa gaagagaaag gaggaagaga ttagggtttt
     9301 gtgccagtaa gacgccccct acaagaatca gaatagaaac atccagaaaa agagaaagaa
     9361 tggtagaagc caaggcatct ataatagagt tagcatctgt gaaccgtgaa atgatttctc
     9421 ctgtacgacg tgtcgcaaag aaagacatgg gaagttcaaa aatatggcga atataggata
     9481 aaatcacatc aatgcttaat ctctggctca gaacggttag gagataatct ctggaaaagc
     9541 tcatgacttg ttggaggata taggtgataa ccagaccaac tgagatgatt cctaaagttg
     9601 atttcatctg atttgggatg tattcatcca agattccttg gagataataa gaaccaccga
     9661 tattgataat agtgaccaat aagcttgaga gaacaatgta agcaatgaga gatttttgtt
     9721 tgaaaatcag aggaaggaag cttagtagac cattcttttt atctttatgg ggttgatagc
     9781 tgggtttggg agctagaaaa atagctactc cagtccattc agagaaaaag cgttcttttg
     9841 acattttagt gatttttaca gaagggtcag gatcaccaat aatcagatag tctttctttg
     9901 tttgatagac aacatagtaa tgttggagtt ttccttcttt gttaacgtga acgataaatg
     9961 gataggggac atcactcatg tcaaagagag ttttatctgc ttgaacaggc cttgtttcaa
    10021 agcccatttc atcagcggct tttacaatgc caagagcagt cgtcccttct ttattggtct
    10081 ttgcaagttc tctcaagtga gctagagaaa aatctgaacc atagaattta gcaatcgagg
    10141 ctaaggcagc aacaccgcag tctctagcat ctatttgagg aacaaatgta cgtttataag
    10201 aagtcattgg caattccttt catatagtgg atagctctat tatgacataa aaacaatttc
    10261 gtgctttata aaatcttgaa tagtcattga aatttcctga atggtaaaaa agtgattaga
    10321 aattatcttt taaaaaaaat ttagaggtag ttttaaataa aaaagctaat ttaagacgtt
    10381 tcgatgacaa ttcaagattt ggatgaaaaa ctttaacaaa tagtgctata ctaaacttgt
    10441 caaagttgca acaagacaaa aattaaaaat aaaaaaggag tatttgttat gaatacaaaa
    10501 atgatggaac aatttcatga aatggatata gctatgttat ctagtattga aggaggcaag
    10561 aataattggc aaactaatgt tttcgaaggt ggtagtgctg cttttggtgg ctgggggtta
    10621 gggacagcta tttgtgctgc gagtggtgtt ggagcgccat ttatgggagc atgtggatac
    10681 ataggagcta aatttggtgt ggctctttgg gcaggagtaa caggggcaac gggtggattt
    10741 taacaaaagg agacttgtat gaatacttat tgtaatataa atgaaacaat gctgagtgag
    10801 gtttacggag gtaattccgg aggagcagct gtagttgctg ctttaggttg tgcagcgggt
    10861 ggagtgaaat atgggaaaat tctaggacca tggggcgctg caataggagg aattggagga
    10921 gcagtggttt gtggatattt agcctatacc gctacatcat aaaaatgaaa gctaaatttt
    10981 atgaaggcta tattctttat tattctcttc gcttttcaaa cctatcttat ttatctatcc
    11041 ataagtatct ctgataaaaa acaaaagact attgaattaa catcattgaa ttgttttgtc
    11101 atattatttc taatctatga taaattaatt tttctcttta ttgcctatgt ttttttgata
    11161 atttttatat taaacttgtt ccgtaactga aaagtgacat actatatgaa tacaaaaatg
    11221 atgtcacaat tttctgttat ggataatgaa atgcttgctt gcgttgaagg tggagatatt
    11281 gattggggaa gaaaaattag ttgtgcagca ggggttgcat atggcgcaat tgatgggtgt
    11341 gcaacaacgg tttgacattt ctattgggac catttgctat aggaataggt gtaactggtg
    11401 ctgcaggtgg agctatactt ggaggtgtag cctatgcagc aacatgttgg tggtaattat
    11461 ggattttaaa agttttatta ttggtttagt agttggtata tttggtcctt atatggatga
    11521 cttaattaga aaaatatttt caaaatcttc caaaaaggat acggatagta ctctctaaaa
    11581 aataaatttc tcccctgtat ttgctagctt aatttacagg ggagtttctt taatattgtt
    11641 tcgcctgtca aaatgatatg tgatagcagg atgaaaagtc tacaaacaag ttggtatcaa
    11701 tacaatataa agaggaaagt atgtttttca tgcttgcttt tttaattttt acaattcaag
    11761 aggttttgat gaccatttat gatttgagtg atccaaggtc aaaataagtg ctatactagc
    11821 agtgtaaatg ttcttgctca actaagataa atcgcacatt taggaggaaa tcttatgaag
    11881 aaaaaaatac tgattatttt cgttctgtat ctgatcatgt ccatctttct ttatccgctt
    11941 agggagagta tttggtataa tctattttat accatagcct atatgattgc ggttatgatc
    12001 tatttttctt taattaaaaa gaaagaaaag aaatgaaaga ttttcttgat taaaaagaat
    12061 catattcttt gtgagattgt tcctgggtca gttgcctttg cttgtttcta cttatctatt
    12121 tctatttcgt cagttttcca atttctttta gtggttatta acttagcttc tattttggtc
    12181 actgtttacc tcactaggga aatgcggata aaagagtttg aagatgatga tttggttagt
    12241 cctagaacca atcaacttat gttcatcggc ttgacaggct ttatgtctat tatttgtttg
    12301 tatagaggga tcacagcagg agaatcctac caacaaataa tcgcttatat tggcgctatt
    12361 ctctgcttga ccatcatgct tctgctcatt tggggattga agtattataa aaagtagagg
    12421 ctaatgcagt ttttgcaatt taagatgtct tgatgacaat tcaagatttg gatgaaaaac
    12481 tttgacaaat agtgctatac tgaaattgtc aaagttgcaa cgggtgctgc tacattatgt
    12541 taaaaatcgc tcttgattga taagttggat ttgttaggaa aggagtaaat aattatggat
    12601 aagaaaaaga tagtttcgac tataatatgt atagtatttc ttgtagtgtc agtagataat
    12661 ttttttagag acttaacccc gttactgttc attttaaata ttattggcct gtcttgcttt
    12721 tcagtgctaa catacattaa cataaaagag atactcttaa atataagtaa atgatgattt
    12781 ggttagtcct agaaccaatc aacttatgtt tatcggcttg gcagatttta tgtttgtcat
    12841 ttgtttgtat agaggtattt cagaaacaga attctatcaa caactaatcg cttatatagg
    12901 tgtattctct gcttgtttat cacgcttctg ctcatgtggg gcttgaagta ttataaaaag
    12961 tagctttcta tccttttctg acaggatatt tgagtagaag gctctattaa attgttagtg
    13021 aaataaatga atggaggtat ttaatatgaa atacagatta ttttttgtta ttttcttgag
    13081 tagtgtgttg gatattcttt tagggacatt tttacaaatc tctatcgtat ctattgggtg
    13141 gcttgttctt tacagtggtt tgtttgaagc aggagttttc cttcttgcta ataaaggggt
    13201 ggcggtaaaa atcaaggaag tagatattcg aaatcgcttt aaatttattt ttggaaaaac
    13261 cttatggttt caaattcttt tgctcatctt tttgataatc aaactttatc ttggtttgga
    13321 tgcgaggttg attttattct atggacatat tttcattgtc tttaatgcct taatgtatct
    13381 attatctagt agtcaggtta gccttaaaaa aaacaaactg tcttcttaat cttacagcag
    13441 tagagcctga caaggtgagg tcatagtagg gaggatttct atgaaaaagt atcaacttct
    13501 attcaaaata agtgcagttt tctcttactt atttttcgta tttggtcttt ctcagatgac
    13561 gcttattatt caaaattatt ggcaattttc ttcccagatt ggcaatttcg tctggattca
    13621 aaatttcttg agtttgctat ttagcggagt catgatttgg attctggtta agacaggtca
    13681 tggttatctc tttcacattc caagaaaaaa atggctttgg tattcgattt tgacagtatt
    13741 agtggtagtg ctccagatct cttttaacgt tcagacagct aaacatgttc agtcaactgc
    13801 tgaaggttgg gctgtattga tcggttatag tgggaccaac tttgctgagc taggtatcta
    13861 tataactttg ttctttctga ctccacttat ggaagagcta atctatagag gattactgca
    13921 acacgccttt tttaagcatt cgagatttgg ccttgatttg cttcttccgt caattttatt
    13981 tgctcttcct catttttcaa gcctgcctag tttgttagat atcttcatct ttgcaacatc
    14041 tggcatcatc tttgctagtt tgacccgcta taccaagagc atttatcctt cctatgcggt
    14101 gcatgtgatc aataatattt tcgcaacatt accatttttg ctgacttttt tacatagggt
    14161 cttggggtaa aaaaacaaaa ggcttgcttt tcagccatag aggagattat catgtataaa
    14221 cacttatttt tcctagattc caaaacttta gatcggttga caccctatat tctagtcttg
    14281 gcttctgaca ccattgcttt taatgttttt gtgctaacct ttgtatctgc ggtggttttt
    14341 aatttcctaa attccatgct agctttaatg gctatattca taggggctgg ctatgtggtc
    14401 ggattttggt tactaatact caatgaaaat caaagagcaa actaggaaac tagccgcagg
    14461 ctgtacttga gtacggcaag gcgacgttga cgtggtttga atttgatttt cgaagagtat
    14521 aaaatggttt gttttggaaa ggctagaact aaagaatgac gcgtaggaag tctgtttggt
    14581 gaggaggata gttttatgga gttttttgat aaatttcatg ccttgtgttt tggattttta
    14641 gtactaataa ttgtcattac agttccttat acgattaacc atggggtttt ttttcaaaat
    14701 gaatctgcat tgattcttgt aagtcttctt gtaacctcgc tgagtgttgc ttatgctaga
    14761 aagtttgaaa tgatttcttt tgggatgtta agcaagaaac aacttttgct tttcattgca
    14821 atctttcttc taagtgtact tgagacgctg gtttatattc atttcttcgc tgtttcttct
    14881 ggctcagggg tccaacactt ggcggaagtc agcagaggaa tttccctgtc tttgattttg
    14941 actacctcag tttttggccc catccaggag gaactcattt tcagaggact tcttcaaggt
    15001 gcggtttttg acaattcttg gttagggctt gtgctaactt cctctctctt ttctttcatg
    15061 catggacctt ctaatgtccc ttcgtttatt ttttatctac ttgggggttt gttgctgggc
    15121 tttgcttata aaaagagtca aaacctatgg gtttctactc tagtccacat gttttacaac
    15181 agttggccac tcctatatta tttataaaaa ttatgaaaag gtaagcagaa gatggtgctt
    15241 acctttttca agctataatg atacccaagc caatccagag gcttttcttt gagaatcggg
    15301 ttagagtggt tgacgaatag gccaaaaact agtagaatag taaggaaact ttatacggag
    15361 gaaagaaatg gatttgggtg ataatgagct aacactgact cccatacctg ggaaaagtgg
    15421 caaggcttat atgggtagct atcctgacgg gaagcgcatc tttgtaaaaa tgaacacctc
    15481 tccaatccta cctggtctag ctagagaaca aattgctcca caattattat ggagtcgtcg
    15541 tttggcagat gggcgtgata tgtgtgctca agaatggttg acaggcaaga tattgacccc
    15601 ctatgatatg aatcgtaagc aaatcgtcaa tattttaacc cgtcttcatc gctcacgtcc
    15661 gttgatgaca caattgagtc gtttgggcta tgccatggaa acacctgtag atttactaca
    15721 gtcttggcag gaaacggctc cagatgcttt gcgtaaaaat cattttatca gtgaagtgat
    15781 ggctgattta cgtcagacta ttccaggatt tagagaggac catgcgacca ttgtccatgg
    15841 agatgtacga catagtaatt ggattgagac agatagtggc ttgatttatt tagtagattg
    15901 ggattcggtt cgcttgaccg atcgcatgtt tgatgtggcc catatgctct gccattatat
    15961 ttcagaacat cagtggaagg aatggttgac ctactacggt tacaagtaca atcaaacggt
    16021 attaagtaaa ttgtattggt atggtcaatt gtcttatttg agtcagattt ccaagtatta
    16081 tatgaaccaa gatttagaaa atgtcaatcg ggagattcat ggtttgcgtc atttccgaga
    16141 caagtatgga aagagaagat gagagttaga aatcgtaaag gggcaacaga attactagag
    16201 gcaaatcccc agtatgtggt cctcaatccc ttggaagcca aggcaaaatg gcgggacttg
    16261 tttggcaatg ataatcccat tcatgtggaa gttggaagtg gaaagggtgc ctttgtttca
    16321 ggtatggcca agcaaaaccc tgacatcaac tatatcggga ttgatattca aaagtctgtt
    16381 ttgagctacg ctttggacaa ggtgcttgaa gttggagtgc ctaacatcaa gctcttgtgg
    16441 gtagatggtt ctgacttaac tgactacttt gaagacggtg agattgatcg cttgtatctg
    16501 aacttttcag atccatggcc gaaaaaacgc catgaaaagc gtcgtttgac ctacaagacc
    16561 ttcttggata ccttcaaacg tatcttgcct gaaaatggag aaattcattt caagacggat
    16621 aaccgtggct tgtttgagta cagtttagtg agcttttctc aatatggcat gaaactcaat
    16681 ggtgtctggt tagatttgca tgccagtgat tttgaaggca atgtcatgac agaatacgag
    16741 caaaaattct caaacaaggg gcaagttatc taccgagttg aggcagaatt ttaagagata
    16801 gcctaaaatt aggctgtaca agtgcttttg ctttacataa gttggcaaac gtgctatact
    16861 gatagtaaga atatgaaaag tgaggcgggg aaatatcttc gcctcttgct tatgaggagg
    16921 tggacgcaat cgcaacaatc gtagaattag tcagagaagt tgtagaacct gtcatagaag
    16981 ctccttttga actcgtggat atcgagtatg gaaagattgg cagtgacatg attctcagta
    17041 tttttgtaga taaacccgaa ggaattacct tgaacgacac ggcagacttg acagaaatga
    17101 tcagtcctgt cctagacacc atcaagccag atcccttccc agaacaatat ttcctagaaa
    17161 ttaccagtcc aggtttggaa cgtcctttga aaaccaagga tgccgtcgct ggagcggttg
    17221 gaaaatacat ccatgtcggg ctctaccaag ccatcgataa gcaaaaggtc tttgaaggaa
    17281 ccttgttggc cttcgaagag gacgagttga ctatggaata tatggacaag acgcgtaaga
    17341 aaaccgtcca aattccatac agtttagtat caaaagcacg tttagcagtt aaattataga
    17401 aaaagaaagg atagcttttg aggattcaaa agtgaagaaa acatgagtaa agaaatgcta
    17461 gaggccttcc gcattttgga agaagacaag ggaatcaaaa aagaagatat catcgacgca
    17521 gtagtagagt cgcttcgttc cgcttatcgc agacgctatg gtcagtcaga cagcgtagct
    17581 attgacttca acgaaaaaac aggtgacttt acagtttata ctgtccgtga agttgttgat
    17641 gaagtatttg atagccgttt ggaaatcagc ttgaaagatg ctcttgccat taattcagct
    17701 tatgaacttg gagacaaaat caagtttgaa gaagcaccag ctgagtttgg tcgtgtagca
    17761 gcccaatctg ccaaacaaac catcatggaa aaaatgcgca agcaaacacg tgccatcact
    17821 tacaatactt acaaagaaca tgagcaagaa atcatgtctg gtacagtaga acgctttgac
    17881 aaccgcttta tctatgtcaa ccttggtagc atcgaagccc aattgtcaaa acaagaccaa
    17941 attcctggag aagtttttgc ttctcatgat cgtatcgaag tttatgttta caaggttgaa
    18001 gacaaccctc gtggtgtgaa cgtctttgtt agccgtagcc atccagaaat gatcaaacgt
    18061 ttgatggagc aagaaattcc agaagtttat gatggaactg ttgaaatcat gagcgtggct
    18121 cgtgaagcag gtgaccgtac gaaggttgct gttcgtagcc acaatccaaa cgtggatgct
    18181 atcggtacaa tcgttggacg tggtggtgct aatatcaaga agattactag caaattccac
    18241 ccagctcgtt acgatgctaa aaatgaccgc atggtaccaa tcgaagaaaa tatcgatgtt
    18301 atcgagtggg tagcagatcc agctgaattt atctacaatg ccatcgctcc tgctgaggtt
    18361 gaccaagtta tctttgatga aaacgacagc aaacgtgcct tggtggttgt tccagataac
    18421 aagctttctc ttgccattgg tcgtcgtgga caaaacgtgc gcttggcggc tcacttgact
    18481 ggttaccgta tcgatatcaa gtctgctagc gaatttgaag ccatggaaga cgctgcttca
    18541 gtagagttgg aagtagaaaa cgatactgta gaagaataaa agctgctaga ggagggaaag
    18601 atgaaaacaa gaaaaatccc tttgcgcaag tctgttgtgt ctaacgaagt gattgataag
    18661 cgtgatttgc tccgcattgt caagaacaag gaaggacaag tctttattga tcctacgggc
    18721 aaggccaatg gccgcggcgc ttatatcaaa ctagacaatg cagaagccct agaggcgaaa
    18781 aagaagaagg tctttaaccg cagttttagc atggaagtgg aagaaagctt ttatgacgag
    18841 ttgatcgctt atgtggatca caaagtgaaa agaagagagt tgggacttga ataagcaaaa
    18901 gataagtaat ctcttggggc ttgctcagcg agcagggcgc atcatatcgg gtgaagaatt
    18961 ggtggtcaag gccattcaag acggcaaggc caagttggtc tttctagctc atgatgctgg
    19021 acccaatctg accaagaaga ttcaagataa aagtcattat tatcaagtag aaattgtaac
    19081 cgtgttttca acactggaat taagcatagc agtcgggaaa tcgagaaagg ttttggctgt
    19141 aacagatgct ggatttacaa agaaaatgag gtctcttatg gaatagaaga ggaggacatg
    19201 atttgtctaa gaaaagattg tacgaaatcg caaaagaact tggaaaagaa agtaaagaag
    19261 ttgtagcgcg tgcaaaagag ttgggcttgg atgtgaaaag ccactcatca agtgtggaag
    19321 aagctgtcgc tgcaaaaatt gctgccagct ttaagcctgc agctgctccg aaagtagaag
    19381 caaaacctgc agcaccaaaa gtaagtgcag aaaagaaagc cgaaaaatct gagccagcta
    19441 aaccagctgt agctaaggaa gaggcaaaac cggctgagcc agttgctccg aaaacagaaa
    19501 aagtagcagc gaaaccgcaa agccgtaatt tcaaggctga gcgtgaagcc cgtgctaaag
    19561 agcaggcaga acgacgcaag caaaataagg gcaataaccg tgaccaacaa caaaacggaa
    19621 accgtcagaa aaacgacggc cgtaatggtg gaaaacaagg tcaaagcaac cgcgacaatc
    19681 gtcgctttaa tgaccaagct aagaagcagc aaggtcagca aaaacgtaga aatgagcgcc
    19741 gtcagcaaga ggacaaacgt tcaaatcaag tggctccacg tattgacttt aaagcccgtg
    19801 cagcagccct aaaagcagag caaaatgcag agtacgctcg ttcaagtgag gaacgcttca
    19861 agcagtatca ggctgctaaa gaagccttgg ctcaagctaa caaacgcaag gaaccagagg
    19921 aaatctttga agaagcggct aagttagctg aacaagcaca gcaagttcaa gcagtggttg
    19981 aagtcgtccc tgagaaaaaa gaacctgcag tggatacacg tcgtaaaaaa caagctcgac
    20041 cagacaaaaa tcgtgacgat tatgatcatg aagaagatgg tcctagaaaa caacaaaaga
    20101 atcgaagtag tcaaaatcaa gtgagaaatc aaaagaatag taactggaat aacaacaaaa
    20161 agaacaaaaa aggcaataac aagaacaacc gtaatcagac tccaaaacct gttacggagc
    20221 gtaaattcca tgaattgcca acagaatttg aatatacaga tggtatgacc gttgcggaaa
    20281 tcgcaaaacg tatcaaacgt gaaccagctg aaatcgttaa gaaacttttc atgatgggtg
    20341 tcatggccac acaaaaccaa tccttggatg gggaaacaat tgaactcctc atggtggatt
    20401 acggtatcga agccaaacaa aaggttgaag tggataatgc tgacatcgaa cgtttctttg
    20461 tcgaagatgg ttatctcaat gaagatgaat tggttgagcg tccaccagtt gttactatca
    20521 tgggacacgt tgaccacggt aaaacaaccc ttttggatac tcttcgtaac tcacgtgttg
    20581 cgacaggtga agcaggtggt attactcagc atatcggtgc ctaccaaatc gtggaaaatg
    20641 gtaagaagat taccttcctt gatacaccag gacacgcggc ctttacatca atgcgtgcgc
    20701 gtggtgcttc tgttaccgat attacgatct tggtcgtagc ggcagatgac ggggttatgc
    20761 ctcagactat tgaagccatc aaccactcaa aagcagctaa cgttccaatc atcgtagcca
    20821 ttaacaagat tgataaacca ggtgctaacc cagaacgcgt tatcggtgaa ttggcagagc
    20881 atggtgtgat gtcaaccgct tggggtggag attctgaatt tgttgaaatc tcagctaaat
    20941 tcaaccaaaa tatcgaagaa ttgttggaaa cagtccttct tgtagctgaa atccaagaac
    21001 tcaaggcaga cccaacagtg cgtgcaatcg gtacggttat cgaagcgcgc ttggataaag
    21061 gaaaaggtgc ggtcgcaacc cttcttgtac aacaaggtac cttgaatgtt caagacccaa
    21121 tcgttgtcgg aaataccttc ggtcgtgtcc gtgctatgac caacgacctt ggtcgtcgtg
    21181 ttaaagttgc tggaccatca acaccagtct ctatcacagg tttgaacgaa gcaccgatgg
    21241 cgggtgacca ctttgccgtt tacgaggatg aaaaatctgc gcgtgcagca ggtgaagagc
    21301 gtgccaaacg tgccctcatg aaacaacgtc aagctaccca acgtgttagc cttgaaaacc
    21361 tctttgatac ccttaaagct ggggaactca aatctgttaa tgttatcatc aaggctgatg
    21421 tacaaggttc tgttgaagcc ctttctgcct cacttcaaaa gattgacgtg gaaggtgtca
    21481 aagtgactat cgtccactca gcggtcggtg ctatcaacga atcagacgtg acccttgccg
    21541 aagcttcaaa tgcctttatc gttggtttca acgtacgccc tacaccacaa gctcgtcaac
    21601 aagcagaagc tgacgatgtg gaaatccgtc ttcacagcat tatctacaag gttatcgaag
    21661 agatggaaga agctatgaaa gggatgcttg atccagaatt tgaagaaaaa gttattggtg
    21721 aagcggttat ccgtgaaacc ttcaaggtgt ctaaagtggg aactatcggt ggatttatgg
    21781 ttatcaacgg taaggttgcc cgtgactcta aagtccgtgt tatccgtgat ggtgtcgtta
    21841 tctatgatgg tgaactcgca agcttgaaac actataaaga cgacgttaag gaagttacaa
    21901 acggccgtga aggtggattg atgatcgact gctacaatga tattaagatg gatgatgtga
    21961 ttgaggcgta tgtcatggaa gaaatcaaga gataagattt tttgctcctt tcttaggtgg
    22021 tgagggacgc aagcaaaccg atggtttcat tgcttatttt tgagcctagg gtctcaaaaa
    22081 tcccctgtga tgggactgat aaatcagttc catcactttc accacggcga aagaagcaga
    22141 tgacttcaaa ttgaacttcg tttcaattta aactgaaaat caagaagttt aaaatagcta
    22201 ggtctgctgg cctagctttt ggttcaaagt agagaaagga atatcatggc aaatcatttc
    22261 cgtacagatc gtgtgggcat ggaaatcaag cgtgaagtca atgagatttt gcaaaagaaa
    22321 gtccgtgatc cacgtgtcca aggtgtgacc atcacagatg ttcagatgct gggtgacttg
    22381 tctgttgcca aggtttatta caccattttg agtaaccttg cttcggataa ccaaaaagcc
    22441 caaatcgggc ttgaaaaagc aactggtacc atcaaacgtg aacttggtcg caatttgaaa
    22501 ttgtacaaaa tcccagattt gaccttcgtc aaagacgagt ccatcgagta tggaaacaag
    22561 attgacgaga tgctacgcaa tctggataag aactaaagaa gaggggttgc ccctcttttt
    22621 tggtggagga aaataggttg aatttgaaat ggaaaaatat tcttttataa tagtttgaaa
    22681 ctagaatagt acgcctctac ttctaaaa
//
LOCUS       DAVBNO010000030        22634 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVBNO010000030 DAVBNO010000000
VERSION     DAVBNO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22634
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.030"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(339..1319)
                     /locus_tag="VQ846_001279"
     CDS             complement(339..1319)
                     /locus_tag="VQ846_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET3075857.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVDDQVYNWLCGSEIPFKYLK"
     gene            complement(1635..3650)
                     /gene="recG"
                     /locus_tag="VQ846_001280"
     CDS             complement(1635..3650)
                     /gene="recG"
                     /locus_tag="VQ846_001280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET3075858.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQVYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3669..4772)
                     /locus_tag="VQ846_001281"
     CDS             complement(3669..4772)
                     /locus_tag="VQ846_001281"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET3075859.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4762..5130)
                     /locus_tag="VQ846_001282"
     CDS             complement(4762..5130)
                     /locus_tag="VQ846_001282"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET3075860.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5169..6200)
                     /locus_tag="VQ846_001283"
     CDS             complement(5169..6200)
                     /locus_tag="VQ846_001283"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET3075861.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(6202..7233)
                     /locus_tag="VQ846_001284"
     CDS             complement(6202..7233)
                     /locus_tag="VQ846_001284"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET3075862.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7314..9827)
                     /gene="secA"
                     /locus_tag="VQ846_001285"
     CDS             complement(7314..9827)
                     /gene="secA"
                     /locus_tag="VQ846_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET3075863.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQSMIKRTLERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9973..10262)
                     /locus_tag="VQ846_001286"
                     /pseudo
     CDS             complement(9973..10262)
                     /locus_tag="VQ846_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10290..10922)
                     /locus_tag="VQ846_001287"
     CDS             complement(10290..10922)
                     /locus_tag="VQ846_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET3075864.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(10926..11567)
                     /locus_tag="VQ846_001288"
     CDS             complement(10926..11567)
                     /locus_tag="VQ846_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075865.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLILSRLEEKFKKG"
     gene            complement(11560..12351)
                     /locus_tag="VQ846_001289"
     CDS             complement(11560..12351)
                     /locus_tag="VQ846_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075866.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            complement(12536..12871)
                     /locus_tag="VQ846_001290"
     CDS             complement(12536..12871)
                     /locus_tag="VQ846_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075867.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(13011..13346)
                     /locus_tag="VQ846_001291"
     CDS             complement(13011..13346)
                     /locus_tag="VQ846_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075868.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13437..14747)
                     /gene="der"
                     /locus_tag="VQ846_001292"
     CDS             complement(13437..14747)
                     /gene="der"
                     /locus_tag="VQ846_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET3075869.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14761..15474)
                     /locus_tag="VQ846_001293"
     CDS             complement(14761..15474)
                     /locus_tag="VQ846_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET3075870.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDVKPRLPLENVVFEEEYQEQSTETIQAYDRVQADYAGGRATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15471..16367)
                     /gene="dnaI"
                     /locus_tag="VQ846_001294"
     CDS             complement(15471..16367)
                     /gene="dnaI"
                     /locus_tag="VQ846_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET3075871.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(16368..17537)
                     /locus_tag="VQ846_001295"
     CDS             complement(16368..17537)
                     /locus_tag="VQ846_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET3075872.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17538..18011)
                     /gene="nrdR"
                     /locus_tag="VQ846_001296"
     CDS             complement(17538..18011)
                     /gene="nrdR"
                     /locus_tag="VQ846_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET3075873.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            18151..18516
                     /locus_tag="VQ846_001297"
     CDS             18151..18516
                     /locus_tag="VQ846_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET3075874.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18521..19216
                     /locus_tag="VQ846_001298"
     CDS             18521..19216
                     /locus_tag="VQ846_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075875.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            19233..20000
                     /locus_tag="VQ846_001299"
     CDS             19233..20000
                     /locus_tag="VQ846_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075876.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20353..21552)
                     /locus_tag="VQ846_001300"
     CDS             complement(20353..21552)
                     /locus_tag="VQ846_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3075877.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21549..22442)
                     /locus_tag="VQ846_001301"
     CDS             complement(21549..22442)
                     /locus_tag="VQ846_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075878.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQEIYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
BASE COUNT         6646 a         5106 c         4192 g         6690 t
ORIGIN      
        1 tttttgaaaa gtattaacac ctacagacaa gcctgacgat agcaagaact accctactcg
       61 ataggtatcg gcttttgctt tctgaaaaaa attattttaa gcaatttgac aaatctagca
      121 acaaaaaatt ttataaatac aatagattga aactagaata gtacaccttt gcttctaaaa
      181 cattgttaga aatcgatttg actgtcctga tcgatttgtc ctgtttttat ttcattttac
      241 tatatttcca tgataaaacg catagtatca agttttctta atcccctgat actatgcgta
      301 tttgtaattt ttaagatttt gtgcttagag tcgactcctt attttagata tttaaaagga
      361 atctcactcc cacagagcca gttgtagact tggtcatcga caaatacatt catggcttcg
      421 tgagcatact caggcatgat gcgataggtt ttatcgcagg tcagacgatt ataaatcgca
      481 aactgggtaa tgggatagca aacatcgtcg tccaagcccg taatcatctt aacctcacct
      541 tggatacgat gggcaagatt tttgacatcg atataggcaa gggtcgccat gatttcctcc
      601 tctgtttcat ggaaggggtc gtgaaacttg aaataacgga aaagttcgtc gtaagcctcg
      661 ctagtattac caatctcaat cacccgtctg aagtctgaca agaagggata aatggcaact
      721 gttttctgaa ttcgaggatt gagcgctgct gcaactagag ctagagcccc tccttgtgag
      781 gcaccatagc tagaaagacg cttctcatca acctgagaca gactagcaac aatttcgacc
      841 aactggtaaa tatccagata aacatcctta taaaagaggt ggtcccgacc ttccatagca
      901 ccacggataa tatgcccctt cacggtattt cctaaaggag aacgcaagcc gtcttgtgag
      961 taacctgact ggccccgcac atccatggaa acaacaccgt aaccagctac ggtgaagccc
     1021 agcatgtcgg cccagtccca gccacgtccc atataaccat gaaaatggaa gattagtgga
     1081 accttctcct cactctttgg aagaacaatg cgtgcataga cctttccttc cttgcttcct
     1141 tcaaatgtta actcatagca cttgacttga ggaatgtgga aatctctttc ctccaagtgg
     1201 taggatggaa gcgtggaaac atttttcact tccccatccc agaaagcatc aaagtcttcc
     1261 ggaacctcat cccttcctct ataggtctta atttcttcta gcaaagctgg atttttcata
     1321 gcatgctcct tttattttgt aatcgttacc acaactgtag catatctatg taatcaatgc
     1381 aagaaagaag gataaataga aagtgaattt agattcttta cttttaggat gataggctga
     1441 aagtagtttt agaaacgaaa aagagcattt cgctctctct tctgtttata ctagtttttt
     1501 gcttccttct tttggaaagt ggtttggtaa tttactttaa tcgttactgt catatgagag
     1561 tcctcgtttc tttttgatga ctttagtata agagccaaac ctaaaaacta gcttaagttt
     1621 tccttagaga aagcttaatc cagatgttct ttcttttcca gatgaagggc aatcatgcgc
     1681 cactctggat cttcttgcca agcttctata gaactaatgt agctagcaac ctttcttgct
     1741 tcttctaaaa tcggaaaatc ttcgataata tcagccactt ggaactctgg aagtcctgac
     1801 tgtctggttc caaaaatctc accagaacca cgcattttca aatcttcctc cgcaaggaca
     1861 aatccattgg tcgtttctgt catgatacgc atgcggtctt tcccagaatc cgtcttggga
     1921 ttagcaacga gaacagcgta ggactgcttg tccccccgac cgacacgacc tctaagctgg
     1981 tgaagttgac tgagaccgaa gcgatcggca tccatgataa tcatgacagt cgcattggga
     2041 acgttgaccc caacctcaat aaccgtcgtc gaaaccagaa tatccgtctt tctctccttg
     2101 aaatcctgca tgatctggtc tttttcgtca ctcttcatcc taccatgtag aagagccacc
     2161 tctgccttgc ctgcaaaatg agtcgtcaac tcctctgata aggcaatggc atttttcaaa
     2221 tctagagctt ctgattcttc aatcaaagga gagatgacat agacttggga acctttttga
     2281 atttccccct ctaaccaagt caagacctga ggtagttgct catgtttgat ccagcgcgtc
     2341 acaataggct tccgacctgc tggcatctgg tcgataatgg aaacatccat atctccaaag
     2401 gctgtgatgg caagcgtccg tggaatggga gtcgccgtca tcatgaggac atctggattg
     2461 tcaccttttt cccgtaaaat acgcctttgc cctacaccaa aacggtgctg ctcatcgata
     2521 ataatcaaac caagacgagc atactccacc ccatcttgta tcagagcgtg agttcctata
     2581 atcaaatcag cctcaccctt ggcaatggtc tccaagactt ctctcttttc tgcagctttc
     2641 aaggaacctg tcaagagagc cagtttcaaa ttgggaaaaa ggttctgtaa actctcaaag
     2701 tgttgctctg cgaggatttc tgttggtacc attagggcag cctgataacc tgctgtcact
     2761 gccgcaaaca tggccaagcc agcgactacc gtttttccac tccccacatc cccttgtagg
     2821 agacgattca tgtggtggtc ggacttcata tcagttaaaa tttcctgcaa actcttttcc
     2881 tgagcttggg tcagggcaaa aggaagactt actttaactg ctgtcacttt ttcctgagac
     2941 caattcagaa ccagaccact tccctgaact ctattttcag acttgagcat ctgcagctgc
     3001 atttggaaat aaaagagttc ctcaaacttt atacggcgaa gagcctgctt gtattctgcc
     3061 aaatcctttg gaaaatgcat agcacggact gcctgacaac gggacatgag tttgtatttg
     3121 tctagtaaag actggggcag attttcttct atcaagaggt ccagtccctg atcaaaagcc
     3181 gtcttgatga ccttgaccag actggcctga ctgattccct gagccagacg atagacaggc
     3241 tggaggtcat cttctacctg agccagaacc ttcatcccag tcagactagc cttagcgcgg
     3301 tcccattttc caaagacagc aagggttgct cccaactcta ttttatcagc cagatagggc
     3361 tggttaaaga aattcaccgc aaaaacgacc tctccctgct tgagactaaa acgcaggcga
     3421 ttgcgcttga aaccataata ctggacacta gcaggagtca ctacctgacc agaaagaact
     3481 gccttctcac cgtcttccag ctccagcacc tgcttggttt tgaagtcttc ataacggaaa
     3541 ggaaagtaga gcaagagatc ttgcaagttt tcaattccta gtttggcgta tttttctgct
     3601 gactttggtc ccacaccagg caagacatgc aagggttgat gtagattcat gctccactcc
     3661 tttcttttct aataatattc tctcggaata cggtcgctga ggaggcaaac cacctcatag
     3721 ttaatggtta cgcggtaggt cgctacctga gttgcagtga tttccttatc cccattggag
     3781 ccaatcaagg ttaccttggt tcctagcgga taaagcttag gcaatcgaat agtgatttgg
     3841 tccatcgaaa ccctgccgac aattgggcaa gcttggccat ctaccaagac agagaaattt
     3901 tgcatgtctc ttgtccatcc atctgcatac ccgattggca cggtcgcgat gacttgctcg
     3961 ctatccgctt gataagttgc tccatagccc atgcaagctc cagctggaac tgtcttgaca
     4021 tgaaccagag cagactccaa ggtcaaggcc ggtatcaaat cataaggcaa atccaagacc
     4081 gctccacttg gattgaggcc atacatggca tctcccatac gaaccgcatt gaaaatagtc
     4141 tctacatgcc aaagagtcgt tgcagaattg ctagcatgaa ccagctctgg aacttccttc
     4201 atactagcta aaatagtttt aaaccgttct aactgggcat taaaatagtc atctgattcc
     4261 tcatcagcag tagcaaagtg ggtaaagatt ccttcaacac aaacaccgtg ttgttggagc
     4321 aaatcttgag cctgctcaac ctcacttgcc tctctaaaac caatccgtcc catccctgaa
     4381 tcaatcttga ggtggactgt caatccagtt aggtccactt ccttatctaa gagtgcttga
     4441 atccactcca gtccagccac tgtcaaggtg aagtcatatt ctttagctag agcaacagct
     4501 tcgatttcag aaactcctaa aatgaggatt ggcttgctga gtccagcttg tctgagttca
     4561 atggcttcat cgatattgga aacgcaaaag ccatcaacat catcttgaat tgccttggca
     4621 acggcaacag ctccatgacc ataagcattg gccttaacca cagccaactt gagcgttcct
     4681 tgagggatat gagcccccat ttgctgaata ttttgtcgaa tagctcccag atgaatcaga
     4741 gccttggttg gtctatgtgg actagctttc atgattttcc tccaaaatga cactggctgt
     4801 cacaaactga tcggtgtggc tgatagacag ccaaatcttt cctgaaaatg gtgcctgact
     4861 aaaataaggc gccccacgtt cattgttcaa gacttccaaa tcctgaaaac cgagcttgct
     4921 aatgcccgtt cccatagcct tggaaaaggc ctccttagcc gaccagcgac cagctaaata
     4981 ttctatttgc ctgcgtcctt tgagactggt gaagcgctcc atttcctgag cggtcagtac
     5041 acgcttagca aatccttcat gtcgtgtaac tgcgctttct atcgaagcca attcttcaat
     5101 gtcaattccg tgtccaacta tcattctcat caaaaggagt tgagattccc caactccatc
     5161 cttttcactt attttgtcaa ggtaacataa atctcttcta ccaaggcctc tgtattttcc
     5221 caacctaggc aagggtcagt aatagagcaa ccaaagatct ctggttggtt ttgacgacca
     5281 tctgctaggt aagattcaat cataaatcct cgaaccgtct ttttaatttt ctcattccaa
     5341 tcacgattct gcaaggtctg gcgaacaatt cgaatctgct ccatatattg cttgcctgag
     5401 ttatcatggt tggtgtcaat gaggataaaa ggattttcaa gtcccatggt ttcatagcgt
     5461 tcaatggctt ggagtagatt ttcatagtag taattcggca tataattgcc atactcgttg
     5521 actgctccac ggaggataac atgggccaaa ggattacctg atgtctcaac ttcctgccca
     5581 tgataaagga aggtttgctt gttttgagca gcatagatgg cgttaaacat aacacccaaa
     5641 tttcctgagg ttggattttt catccctact ggtgcatcaa tcccagaagc cacaaagcgg
     5701 tgctcttggt cttccacaga acgagctcca acggcatggt agctgaccaa gtcatccacc
     5761 aagatcagat ttgacggata aagcatctca tctgccgttg tcaaaccagt ctctgtaatc
     5821 acgcggtagt gcaactggcg cacagcctgc aagccattaa tcaggcttgg agccttagaa
     5881 gtatctggct ggtgaactaa tcctttatag ccgtctccat tggtacgagg cttagcagta
     5941 tacacgcgca tgaccatgaa aatcttatcc gctaccttct tttgcaaggc agataaacgg
     6001 cgagcatatt ccaagaccgc ctcttcatta tcagaagagc aaggaccaat caccaagaga
     6061 atacggtcat cttcccctga aataatatct gccaattccc tgtcacggct ttccttgagt
     6121 ctcaaggctt ccgcagacaa ttgggtttca gccttgatga cttccatatc gatttcttga
     6181 cctttttcaa taaatgccat cttattctcc taacgttttg taaatttctc tgacaagagc
     6241 ttctgtgtta tcccaaccca ggcaagggtc tgtgatagac ttaccaaata cttctggctc
     6301 attttgtcga ccatcttcca gataagactc aatcataaaa ccacgaacga actgcttaat
     6361 tttttcattc caagcacggt taatcaaggt ctggcggaca attcggatct gttcaatata
     6421 ctgcttacca gaattgtcat gattggtatc aatgatgata aaaggatttt ccaagcccat
     6481 tttctcatac tgggcaatgg tatcaattaa attgtcataa tagtagttgg gaatattttt
     6541 tccatactca ttaagagcac cacgaagaat agcgtgtgaa agcgggttcc cagttgtttc
     6601 tacttctttt cctaagaaaa ggaaactttg tttgttttga gcagcataaa tcccattaaa
     6661 catgacattg agatttccag aggttggatt tttaaaacca gtagaaaatc ctgccccact
     6721 tgccacaaag cggtgttgct ggtcttcaac tgaacgggca ccaactgcca tgtaagaaat
     6781 caaatcatct acaagcggaa ggttttcagg ataaagcatt tcatcagcag ttgtcatccc
     6841 tgtttctgtg atgacacgat agtgaagatg gcgaacggct ttgattccat tgataagact
     6901 aggcgcttct gtcgcgttag gctggtgaat caagccctta tagccatctc cgttggtacg
     6961 gggtttggca gtataaacac gcataaccat aaagatacga tctgccactt cttcttgtag
     7021 gactgccaaa cgcttagcgt attcaaggac agcttcttcg ttgtcagatg agcatggccc
     7081 gattaccaag agaattcgct ggtcttctcc acgtataatg gcttctagct cttgatctcg
     7141 ctgtgatttc ctctccaaag cctgaccttc taattttgac aaggcacgaa cttcttctat
     7201 atttatttta gagctttttg ctgtaaatac cataactcat ctccttataa agcaaacgga
     7261 tacccagtaa aaatttactt ttcacaagat atccgcctct aaactatctc attttattgt
     7321 cttttaccgt gacagttttt aaatttctta ccagaaccac atgggcaaag ttcattgcgt
     7381 ccaatctggc tcaaatccaa atcttctggc atacttgctt ggtgagcagc gatattgcga
     7441 gtcgctgttg tactgatatg acgttctgcc tgtggtcttt cttgttcatg aatttgtgct
     7501 ttcatcatca agcgtgtcac atcaaactca atcgaaccaa tcatatcatt aaacatacgg
     7561 aaaccttctg cctgatactc aacaacaggg ttgttctgag catagccacg aagtccaacc
     7621 gcgttacgca attgatcaag ggcatcgata tgatctgtcc acttgttatc caccactcgt
     7681 agaatcaaaa ctttttggaa ttctttaact gcttcttcat cgcgtagttt tgaaacctga
     7741 ctatcgtaaa ccttcaaggc acgttggaaa agctcttcct tgatggcctt atcagacaag
     7801 cctgacaagt cttccatcgt aatagaatct tcaggaagca agttgtactt agcaaagttc
     7861 aaaattgcct ctagtttttc atcttgtttg gcacgcgcat gaccatcaac gacacgttca
     7921 agcgtgcgct taatcataga ctgaatttca ggtgccaagt cacggtctgc agtaatgaca
     7981 tcataacgtt gagtatagat aatctcacgt tgttcacgca tgacatcgtc gtattgaagg
     8041 acttgtttac gggtatcgta gttatttcct tcgacacgtt tctgagctgc ttcaacctga
     8101 cgcgtcaaca tgcgagactc aatcgcctct tcagacatgt tcaagcgttc aaagattccc
     8161 ttcaagcgtt cagaaccaaa acgtttcatc aaatcatctt caagagatag gtagaattgt
     8221 gactcacctg gatctccttg acgacctgaa cgtccacgaa gctggttatc gatacgacga
     8281 ctttcatgac gttctgtacc aataacacaa agtcctccaa gttcacgaac accttcacca
     8341 agcttgatgt cggtaccacg acccgccatg ttggttgcga tggtaacggc accacgttga
     8401 ccagcattca tgatgatttg ggcttctcta tagtggtttt tggcattcaa gacttcgtga
     8461 ggaacaccag ctgcaaccaa tttcttagaa atgtagtcac tagtttcaac cgctactgta
     8521 ccaaccaaga caggttgacc cttttggtaa cgagccttaa cgtcttcgac aaccgcttta
     8581 aacttagatt ctatacttgc ataaagaagg tctgagtggt caatacgttg aacaggacgg
     8641 tttgttggga ttggaataac acgaatgttg tagatttcac ggaattcttc ttcctcagtc
     8701 ttacctgtac ccgtcatacc agacaatttc ttgtacatac ggaaaaggtt ttggtacgtg
     8761 attgaggcag atgtcttggt ttcatcctgg attggcacac cttctttggc ttcaatagct
     8821 tggtgcaatc catcagaata acgacgacct tccatggtac gacctgtaaa ttggtcgaca
     8881 atcaagattt cttgctcttc gctcaccaca tagtcaatat cgagaagcat gatgtagttg
     8941 gcacgaaggg cgttatcgat aaagtgggtc aaagccacgt tttcgatgtc atagaggttt
     9001 tcaagtttga agtagctttc agccctgtca atccctgaat cagacaaacc aatagtctta
     9061 gactgcacat cgatgatgta gtcatctttg ttcaaagatt ttacatagtg gtctgccatg
     9121 tgatacaact gactggtttc aaccgcattg gcacctgata caatcaaagg tgtacgagcc
     9181 tcgtcaatca agatagagtc aacctcatcg accaaggcat agttaagcgg acgttgtacc
     9241 atgttttcag cgcgaacgac catattgtca cgaaggtagt caaatccgat ttctgagtta
     9301 gttgagtaag taatatcaca ctcataggct tcttttttct ccattggaga tttggtagcc
     9361 aagttaatcc ctactgacaa accaagccaa gagtacaatt cacccatctc agtcgcgtca
     9421 cgttctgaca ggtattcatt aaccgtaact acgtgaaccc ctttacctga aagggcattg
     9481 aggtataccg gcatggtcgc agtcaaggtt ttcccttccc ctgtacgcat ctctggcacg
     9541 tcaccatggt gaagaacaat cccccccatg acctgaacct tataagggaa gagacctagg
     9601 acacgtttgg caccttcacg gacaaccgca aatgcttcgt aaagcaatga atccagtgat
     9661 tctccatttt gataacgttc cttaaattca actgtttttg cttttagttg gtcgtcagtc
     9721 aaagcagcca tttggtcttc gtatttgaaa accttgtcag ccatcttttc cagacgacgg
     9781 atttctcctt tatcattttc gataattgtt tttaaaatat tagccatgtt tttccttact
     9841 ttaaattccg aatattttag aatgttcctt taattttagc acaattactg attattttca
     9901 aggaagaatc cccattttga aaaggaaaaa gaggttagtt tccaagctaa cctctctgac
     9961 ttttatgata ctttaattgc caagaatcgg caaaatagca aataggataa ataagttaat
    10021 ccaaagagaa agacagcaga tcaatccaaa aacaaagaga ctgactttct tggacagcaa
    10081 ggccatcaaa atggacagaa tgccaaaaac tagcaagact ttctgtatgc tgaagaggtc
    10141 aatcttcccc cccccagaat cggactgccc caaggaagaa agaagaaagc aatccagatc
    10201 aacagaacta aaccaatata gaccttagaa tagcgtgtaa taaatgattt tagatgtgcc
    10261 ataagccatc tcctaggatt gtatatcctt tacatttgta tatgtccatt ttcgacaact
    10321 aatacatctg tacaaaggtc tgaaatatct tccgatatgt gcgacgtcag gataaccaga
    10381 ccattttctt ttttcaggta agataaaatg acctgtttgg ttaacctcac acttttctca
    10441 tccaaagcat tcataggttc atcgagaaag agtatagaag gagaggaaat aaaggcttga
    10501 atcaaggcca ttttttgctt tgttcccaag gataaatgcc ggtattcaac gtcttcaaat
    10561 tcctgtaaat catagtattg aatccaatag gcaattcttt tttccgtaac tttagatgaa
    10621 aaatacctta acagttccaa attttctctc agggataaat gagataaaaa ctcgactttt
    10681 tcaattaaaa ttcctgcatc ctgaatataa tgatttttta tcccgtaaac tttaccatct
    10741 tgaagaactt ttcctttgtc aagcttaata taaccagcaa gtatctttaa aagaaccgtc
    10801 ttgccagatc cattatcacc tttaagtcca taaatttttc ctgattgaaa caccaaattc
    10861 aactgattta aaataggtct tttcccataa attttctgtc catttataat ttcaagtctc
    10921 atcgtttatc ctttcttaaa tttctcttcc aagcgactaa gaatcaacat aactaataag
    10981 cttaagagta ctaaatacac tgttacaata tgatccatca tccattcttg tcccacaaat
    11041 gccaaaaaat agagaaaaaa agaaaaacca gcgcccgcac taatcagtgc tatcagataa
    11101 gacagtagta agtacaacat cattaactga gcaagtaaaa agaaaagtgc gactgttttg
    11161 aaatgaattc ctagcggaag taaaccaaga aaccagacaa acaaatcttg agtaaaaaat
    11221 ttagaaatga caaccaatcg tttccttgta taatcgaaaa aggagcttga ttgatagcga
    11281 atcaagtgat ggtaatggtc tgccaaatca ctttcaagta aaatgacaga gagccatcct
    11341 accatgaaaa gcgaaaggtt taacaagagt ttggggattt ctaaattcgc ttcagaatag
    11401 tcattatagg aaaaaattga aatcaattct ctcttgaaaa agataacagc atcctgcttt
    11461 tgaaccgctg ttgacaatag atagtttatc aaaatcatca tcagaactat acgaagtagt
    11521 aaaacgatct ttctctttag ccaaatattg ttaaaattct taaacattat cttcatattt
    11581 tttaagtacc atataaggga caacataaag gatataagga gtaaagagtt tcatcaaact
    11641 tacatcccca tagctagctt gcatcaaact cgtcatagga accatatagt aaggcaaggc
    11701 actatagagc agcatagaac caagccaaag aaacatcaaa taggtgattg ccgaacgagt
    11761 cacattcccc acataatcaa ctatttgtaa aaaatagatt gcattgatga aaataccgat
    11821 tagtaggaca caagtaaaga acaaatagct ttttatctct ccatctagga gtctttgtaa
    11881 accactccca tcagaaaata gagaattcca ttcaagagga gaaaaagtcc caaagaaata
    11941 ggttataatt gcaattatca gcacagtcac taaatatatc aaacaaggga tacttgcaac
    12001 ttgcaaagtc aactttcttt ttaacccttg atagctcact tctcttccaa tactcaatcg
    12061 taaaacttta tttttcagct caatatattg aaaacctagc aaaacaatga tgattggaat
    12121 aataaatgat tgataaaaac ctagattgaa ctggagaata cgaaaatcta atgctgattt
    12181 taataagtca tttttaattg cttgtccatc gaatttagct agatcgggcg catgaatatc
    12241 aaaaaagaaa taagtcatca aaacatcacg ataaaccaaa gcaagaaaaa gcaaagtaag
    12301 caagacataa acaaatttat gccttgccaa agttaagaga ggaatcttca taagccacct
    12361 cctacaaata gaaagtaata taatactcaa tgaaaatcaa agagcaaact aggaaactag
    12421 ccgcaagttg ctcaaagcac tgctttgagg ttgtagataa gactgacgaa gtcagttaca
    12481 tatataatcc aaggcgacgt tgacgtggtt tgaagagatt ttcgaagagt ataaatcaat
    12541 gccttccgcc tttagacttc cctagttccc gtctcaagtc aagcattttt ttgaaacaga
    12601 tatagtctaa ccagtcacta gcaaaagcga taagtagtaa taatagccaa ataccccata
    12661 acttgatatc tgtcaaattt ctcaagacga tattgaaaaa cagaactgaa aaaactgtcc
    12721 aagcaaggct aaaaagagca tagagggtga tgtaaatcca gtaaaaatag taaaaaattg
    12781 ggaaaatctt actatctctg tttgcctttt caatccagct gaaaaagtaa aaacagggca
    12841 ataagattat cattttaaac aagtattcca tcacaaacct cctcttttaa attgttttct
    12901 ttagtttcgt tctattaaat gttattacca atataaacct tggaatagcg tgtaataaat
    12961 gattttagag gtaccataag ccacctccta caaatagaaa ccgatataaa tcaatgcctt
    13021 ccacccttag acttccctag ttcctgtctc aagcgaaaca tttctttgaa acaggaataa
    13081 gttaaccaat tcataccaat agctagcaga ataaaaagaa accaaatgcc ccataacttg
    13141 atatctgtca catttctcaa gacggtattg aaaaacagaa ctgaaacaac tgtccaagca
    13201 aggctaaaaa gagaatagaa ggggatgtaa aaccagtaaa aataataaaa aattggaaaa
    13261 aacttactat ttctgttggc cttctcaatc cagttatcaa aataaaagta cggtgctaaa
    13321 agtaagaatt taaacaaatg ttccatcacc gacatcaccc tccttctttt gatagcgttt
    13381 tctattattt tattatatca aaaaaatccg gaactgtcat tccagattct acttttttat
    13441 ttgcgttttc ttgcgatgag atgaatcggt gttccctcaa aaacaaaggc cttgcggatt
    13501 tgattttcca agaaacgcag gtaagaaaag tgcatgagtt cttcttcatt gacaaagatg
    13561 acaaaggttg gtggtttggt tgccacttgg gtcgcataga aaatcttgag acgttttcct
    13621 ttgtctgtcg gtgttgggtt gatggcaatg gcatccatga tgacatcgtt caagacagct
    13681 gatggaatac gtgtattttg actttcgctg atttgcttaa tcatctcagg aagtttgtgg
    13741 agacgttgct tagtcaaagc tgatacaaaa ataatcggtg cataaggcag gtattggaac
    13801 tgctcacgga tatcttcttc ccagtttttc atggtatggt tgtctttttc aagcgtatcc
    13861 cacttgttga ctacgataat cattccttta ccagcttcat gggcaaatcc tgcgatacgc
    13921 ttgtcgtact cacgaatgcc ttcttccgca ttgatgacca tcaagaccac atctgaacgg
    13981 tcaatagcac gcatggcacg cataacagag tatttctcag tattttcata aaccttacca
    14041 gacttacgca taccagccgt atcaatcatg gtaaactctt gaccatctgt atctgtaaag
    14101 tgggtatcaa tagcgtcacg agttgttcca gcaacaggac tggcaatgac acggtcttct
    14161 cccaagatag cattaatcaa gcttgatttt ccaacgttag gacgaccaat caagctaaac
    14221 ttaatgacat ctggattttc ttcttcatat tcatttggaa ggttttccac aatcgcatct
    14281 agtacatccc ctgtaccgat accatggaca gatgagatag gcagtggttc acccaaaccg
    14341 agagcataga aatcatagat atcatttctc atctcagggt tatccacctt gttgactgcg
    14401 aggataactg gtttgtgggt cttataaagc ttgcgggcta cgtattcgtc tgcatcggta
    14461 attccttcct taccggacac gacaaagacg ataacatctg cttcttccat ggcaatttct
    14521 gcctggtgct tgatttgttc catgaaagga gcatcgacat catcaattcc tcctgtatca
    14581 atcatgctaa aagaacgatt gagccactca cccgttgcat aaatacggtc acgtgtcact
    14641 ccttcgacat cttctacaat ggagattcgc tcaccagcga tccgattaaa tagggttgat
    14701 ttcccaacat tgggacgtcc tacaatggca atagttggta gggccataat ttctcacttt
    14761 ctacaataat ttcttctgtt caagattttt tctagttgag cttggttcag cttgaccaaa
    14821 ctgttctgct aggcgctgac tccagcttgt ggtcgcacgc cccccagcat agtcagcctg
    14881 cacacggtca taagcttgga ttgtctcagt tgactgttct tggtattctt cctcaaagac
    14941 aacattctct agtggcagtc tcggtttcac atcatgatgt tgatttggca cacccagtgc
    15001 catcccaaag acagaatagg tgtagtcagg taggttaaag agctctgcca cttcttcaga
    15061 cttgtatcga accaaaccga taatcacacc accatagccc aagctttcag ctgccaacaa
    15121 ggcgttttgt ccagcaagag ctgcatcgac cgaactaatc aagagacctt ccacaccttg
    15181 gggttggaag gtgtcggtat gaagtcgggc tcccttttct gctcggttca aatctccgac
    15241 aaagagaagg aaaacagcag actggcgaat ggcttcttga ggtaccaatt catacaaggc
    15301 atctttcttc tcttgacttc gtaccacaat cacagagtag gattggaaat tcttccaaga
    15361 tgatgccatc tgggctgctg tcaaaatctc atttaagtct acttggggaa tttcttgctc
    15421 tttaaacctg cgcactgaag tatgagcctt catcaattta atggtttctg tcatcgacgg
    15481 tttactcctt ctaaacgagt ctcctcagcc aaataacgga tgcgttccat gacccgtctg
    15541 gcttcccagg tttcgtcatt tccatgtttc actttcgcaa aatgcttctc caaatcttca
    15601 aagttgaagt tggatgtgaa aaaggtcggt aaattttcct gcatccgata ttggagaatg
    15661 acctgcagga tttcgtcacg tacccaaacg gttgattgct cggcgccaat atcatctaaa
    15721 atcaggacct cagacagctt aatctcatcc accaaggtct taacattgcc atcactgata
    15781 gcatttttga catcaatgac aaagctagga tagtggagga gagttgatga aacaccacgt
    15841 ttttctgata aatcatgagc taaggccgcc accatgaaac ttttacccac accaaagtct
    15901 ccatataagt aaagaccttt tcgaatagct ggatattgct ccacgaaggc tagtagcttt
    15961 tcaaaaactg gtaagcgccc caaatcatcc aagtcaactt gagccaaact agctttcttg
    16021 agactggctg gtagattgat taacttgaga cggttcttaa tagccgcttc tttttcagcc
    16081 gcgattagct caggagtttc ttcatatgaa acatctgcat agccatgatt cttaaccaaa
    16141 atcggcttgt agcctttggc aatataatcc gtatccccac ggagaaactt gtcacgctcg
    16201 gtgatgtact gattaaactt ggagatactg cgatttaatt cctctggagt caaggattct
    16261 tgctggataa aagccgcaac atcagggtcc ttcatgattt tctggaccaa atcttgataa
    16321 taaaaacggc tgggttgacg tttgagtacg tctccgacac tttccatcta atctcctcct
    16381 ttttctaatc gagctaatag ttcttgcttc ttacgttcta gttccagacg agtttcctcg
    16441 ctggtttcat tcttatattc aggattactc catttaggaa cattggtttt tactggggca
    16501 gtctgattct gtttttgtgt ttttgctttc tgtcctcgct cacggatgcg caagactgcc
    16561 ttttctgccg aatgaatctt ttgataggca tagtcattgg ctaccttcat ggcatatttc
    16621 tcattgatat ttgccgaatc gaccttatta aaggtcaata aaagaatgat attgatgact
    16681 tcgtccagca agcccaagcc agccatctgt tgcaagagtt ctctttctgt ttgggtaatg
    16741 gttcccttgc gtgtttgctt gatttctgcc aagaactgca gggcagtttt acttttggct
    16801 tctttgataa tggttgtttc cttaagacta aagtcagagg aaactggttt ttgagcaatt
    16861 ttttcacgca tgcgtttggt tgaaataacc tgagaaacag ctgttgactt ggccaattga
    16921 taggtttcaa accaagtcca tttcttctcc tcggcaatag caaagaggtt taagacatcg
    16981 gactgctcat ccgcaaaacg aagtccatct cgagccatca gctggcgaaa atgttccaag
    17041 tcaaaatcat tggccacttt cttcttgaga ccaaggtctt cttgactgcc tagttctgcc
    17101 aattctggaa agacttgatt gagtgagaca ggtatttctt caccatcagc actttcaact
    17161 ttcaaatcct ccacagcaga gtcgccaatc tttttctcta agagtctgcg ataaacagga
    17221 tgctccaaga agtcttgact agatagagga gcatggaggg ctagctgata aacatccccc
    17281 ttttgataga gggtcaagag attaaaagca gataagattt tcaatgattt tatcagtcta
    17341 tccatcccaa agttgagatg attgaggata cttgaaaaaa gatattcctt tctaccatta
    17401 tcccaaaaac tgattgtata aagataaagg ctcagtgcct cctgaccgat aatcgggagg
    17461 tagcactgta ccagagatga ggtatcttgc gacacccgat tattctttag ataagaaaaa
    17521 cggtcaattg gcttcattta tctttccttt ttctttttag aggactgggt gatttgttgg
    17581 agcaagctct ctaactcact gacatcctta aaactacgat agacactagc aaaacgtaca
    17641 taggtaatct cgtccaattc agccaactcc tccatgacga gtgaaccaat gtcctcactt
    17701 tgaatttcat tttcatttcg accacggagt ttctgttcga tacgattgac taccatgttg
    17761 atttcatcac ttgacacagg acgtttctgg gctgagcgga taatcccatt aaagatttta
    17821 tctctggaga attgttcccg tgtaccatct tttttaacaa ccactaaggt tctttcttct
    17881 actcgttcgt aggttgtaaa acggtgttgg cattcgtcgc actcacgtct tctacgaatg
    17941 gtgttccctt cttctgcttg gcgactatcg ataacacttg acttggtagc cccacatttt
    18001 ggacaacgca tcctttccct ccttatcgtt ttcttttcat tataccattt tttaagcgat
    18061 tcccaaaaca attcttcttt ttgcttgaca agttttttgt tttgttgtat tatttaatta
    18121 agacaaaagg ataagagaaa ggagactaag atgtcctgga catttgacaa caaaaaaccc
    18181 atctatttac agattatgga gaaaatcaag cttcagattg tttcccatac actggaaccc
    18241 aatcaacaac ttccaaccgt gagggagcta gctagcgagg ctggtgtcaa tcccaatacc
    18301 atccaaagag ccttatcaga ccttgaacga gaaggatttg tctacagcaa gcgaacaact
    18361 ggacgatttg tgactaagga taaggagcta atcgcccagt cacgcaaaca attatcagaa
    18421 gaagaattgg aacacttcgt ttcctccatg acccattttg gctatgaaaa agaagaacta
    18481 ccaggcgtag tcagtgatta tattaaagga gtttaagcct atgtcattac tagtatttga
    18541 aaatgtatcc aaatcatatg gagcaacacc agcccttgaa aatgtttctc ttgacattcc
    18601 agctggaaaa attgtcggcc ttcttgggcc aaacggctca ggaaaaacaa ccctgattaa
    18661 actaattaat ggcctcttac aaccagatca aggacgtgtc ctcatcaacg acatggaccc
    18721 aagcccagca accaaggccg ttgtagctta tttgcctgat acgacctatc tcaatgagca
    18781 aatgaaggtc aaagaagccc taacctactt caagaccttc tataaagatt tcaatcttga
    18841 acgcgcccat catctacttg cagacctggg cattgatgaa aatagtcgtc tcaagaaact
    18901 atcaaaagga aacaaagaaa aggttcaact gattttggtt atgagccgtg atgctcgtct
    18961 ctatgttttg gacgaaccca ttggtggggt ggatccagca gcccgtgctt atatcctcaa
    19021 taccattatc aacaactact caccaacttc taccgttttg atttctaccc acttgatttc
    19081 tgatatcgag ccaatcttgg atgaaattgt cttcctaaaa gacggaaaag tcgtccgtca
    19141 aggaaatgta gatgatattc gctacgagtc aggtgaatcc attgaccaac tcttccgtca
    19201 ggaatttaag gcctaagcaa aggagattat ttatgttttg gaatttagtt cgctacgaat
    19261 ttaaaaatgt taacaagtgg tatttagccc tctacgcagc cgtgctagtc ctttctgccc
    19321 tcatcggaat acagacacaa ggctttaaaa atctacctta ccaagaaagt caggctacta
    19381 tgctactttt tctagctaca gtctttggtg gcttgatgct tacacttggg atttcaacca
    19441 ttttcttgat tattaaacgc ttcaaaggta gtgtctacga ccgacaaggc tatctgactt
    19501 tgaccttgcc agtttctgaa caccatatca tcacagccaa actaatcggt gcctttatct
    19561 ggtcattgat tagcaccgct gtattggctc taagtgctgt tattattctg gctttaacag
    19621 ctccagaatg gattcctctt tcttatgtga ttacatttgt agaaacacat ctccctcaga
    19681 tctttcttac aggtatatcc ttcctactaa atactatttc aggaatcctc tgcatctacc
    19741 tggctatttc cattggacag cttttcaatg aataccgtac agcactcgct gttgcagtct
    19801 acattggtat ccaaatcgtc attggattta ttgaactttt cttcaatctt agttctaatt
    19861 tctatgtcaa ttcactggta ggactcaatg accatttcta tatgggagca ggtatagcca
    19921 ttgttgaaga actcatattc atagctatct tttatctcgg aacctactac atcttgagaa
    19981 ataaggttaa tttgctttaa ataattttta cctagatatg taacatactc atagaacaaa
    20041 agagaccagg caaaaagtct ttacaattag aaaacgcata gtatcaagtg ttaaaaatcc
    20101 ttgatattat gcgttttttg tgagctgact tatttccttt cactatatcg caaaatgaaa
    20161 taagaacgga acgatgggat tttggaattc aaatcaattt ataagaatgt tttagaagta
    20221 atattatcct attccagatt cagttcacta tacaattgag ttttcaagca acctgtttac
    20281 ataatgtgta cataattagg ttcgtgattc cacccttttc acctttaaaa acctcgcttt
    20341 cgcaaggctc ttctatttat aagataaggc acgtttaaag gttttccaaa tccctaaatc
    20401 atccgtttga agaacgagac tagcatacat gcgtccgata aatcctgttg ctaccaccgc
    20461 aaaaatcact gtaatagcaa gtgaaatcca tgcttccgct ccctccgcat agccattaat
    20521 cgttcgaaac ggcataaaga aggtcgaaat aaagggaata taagaaccaa tcttcaagag
    20581 gagattgtca ccagctgcac ctagagctgt cactccaaaa aaaccaccca taatcaaaat
    20641 catcaaaggc gacaaggctt tccctgagtc ttcaggacga gaaaccatag atcctaggaa
    20701 ggctgccaag actacgtaca tgaaaagact gatcaaaata aagagcaagg tattcagtga
    20761 gatagcatct cccaagtgat ccaaaatacc agactgagcc aagaatggca aatctttaaa
    20821 gagcaaaacg gcagccagac cacctacaac atagatccca atatgcgtta aaatcactag
    20881 aaacagagcc atcatccgcg catagaaata gtgacttgcc cttatgctag aaaagacgac
    20941 ttccataatt ttggtgcctt tttcactggc aacttcctga gctgttacac ccgcataggt
    21001 aatcagaatc atataaagaa agaatcctaa ggcacctgct gcaattgttt gaataaactt
    21061 tttattttcc ttggcttcat caatcttttc tgtgaattga attgtctgcg ctaagcgttt
    21121 ttcctgctct tgagacaagg aagcagttga acgattaagc tgattttgca gttcattgag
    21181 tgtacctgta acctcaaatt taattccatt ttcaagcgat gtttcgccat gataaactgc
    21241 ctttagaaca ctatcttctt gatcaatggt caaataacct tttaattttt cttctttaat
    21301 tgcttctttg gcacttgctt cgtctttata gtcgaagtta acaccattta cattcttcag
    21361 tccttctgct acagatggca ctgttgtcac tactgccact ttattatttt tagccataga
    21421 agaaccttgg agatgcccaa ttcctacaga gattcctaaa aagaggaacg gcgaaatcac
    21481 cataaagaag aaactccatg actcgacatg tcgaagatag gtttccttga ttacaaccca
    21541 catatttctc atacttccac tcctgattct agtttaaaga tttcatcgat agttggcgct
    21601 tgttggtcaa atgttgcgat atattgacct tgagtcaaga ttgggaagag ttcccttcca
    21661 gcgctctcat cctccaaaat caatttccaa ctgccttgtt tggtcaagct cacctgtttg
    21721 acatgaggaa gattttccaa ttcttccttg cttcgttcac ttgaaacaaa gagacgcgtt
    21781 ttcccgtatt gattgcggac atcctgaact ggtccgtgca agaccacacg gccatctcga
    21841 atcatcagaa tatcgtcaca aagttcctca acattggtca tgacatggtc agaaaagata
    21901 atggttgctc cgcgctcttt ttcctgaaaa atgacttgtt tgagcaattc tgtattaact
    21961 gggtccaatc cactaaaagg ctcatccaag ataatcaggt ctggttcatg aatcagagta
    22021 ataatgagct gaatcttctg ctgatttcct tttgacagac tcttgatttt atctgtcagc
    22081 tttcctttta cttccaacct cttcatccat tgagggagtt tttctttgac ttctttggca
    22141 tccatgcctt ttagagtcgc caagtagcga acttgttcaa gaactgtcaa tttaggcatg
    22201 agactgcgtt cttcaggcag ataaccaatc cgagcataga tctcctgacg aatatcctga
    22261 ccatccagac cgatttctcc ctgatattct aggaatttta aaatactatg gaaaatcgtt
    22321 gtttttccag caccattttt tccgactagt cccaaaatac gacctggtcg cgcttgaaag
    22381 tcaataccaa acaaaacttg cttggatcca aaacttttct ctagacttct tacttctagc
    22441 atctttcacc tccgaaattt cttgcactca ttatactcct ttttgatagc ctttacaatg
    22501 ttttttgtcc atttttagaa gactattgct gtgtaaaata tggcctggag cacttttata
    22561 ctcaatgaaa atcaaagagc aaactaggaa gccagccgta gactgctcaa agtacagctt
    22621 tgaggttgca gata
//
LOCUS       DAVBNO010000031        21874 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVBNO010000031 DAVBNO010000000
VERSION     DAVBNO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21874
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.031"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(408..1604)
                     /gene="tuf"
                     /locus_tag="VQ846_001302"
     CDS             complement(408..1604)
                     /gene="tuf"
                     /locus_tag="VQ846_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET3075879.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2480..3349)
                     /locus_tag="VQ846_001303"
     CDS             complement(2480..3349)
                     /locus_tag="VQ846_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET3075880.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3572..4252)
                     /locus_tag="VQ846_001304"
     CDS             complement(3572..4252)
                     /locus_tag="VQ846_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET3075881.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDK
                     PTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGF
                     FKKKEDEK"
     gene            complement(4210..5316)
                     /locus_tag="VQ846_001305"
     CDS             complement(4210..5316)
                     /locus_tag="VQ846_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET3075882.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWVRSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELILENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5427..5681)
                     /locus_tag="VQ846_001306"
     CDS             complement(5427..5681)
                     /locus_tag="VQ846_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075883.1"
                     /translation="MLNVDQDFMSISKPNKSGSDWKKTFTVRTTNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(6039..6572)
                     /locus_tag="VQ846_001307"
     CDS             complement(6039..6572)
                     /locus_tag="VQ846_001307"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET3075884.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     RAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(6559..6774)
                     /locus_tag="VQ846_001308"
     CDS             complement(6559..6774)
                     /locus_tag="VQ846_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3075885.1"
                     /translation="MGKERCEQGHIERKKRVVKNRKIPLEELKAFVEAHPDAFLREIA
                     AHFDCALPSVWAALKYIKVTLKKDDEL"
     gene            complement(6741..6884)
                     /locus_tag="VQ846_001309"
     CDS             complement(6741..6884)
                     /locus_tag="VQ846_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HET3075886.1"
                     /translation="MAYSTDFKQRALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KDT"
     gene            7118..8836
                     /locus_tag="VQ846_001310"
     CDS             7118..8836
                     /locus_tag="VQ846_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET3075887.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8947..9294
                     /locus_tag="VQ846_001311"
     CDS             8947..9294
                     /locus_tag="VQ846_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET3075888.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     SGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9572..10408)
                     /locus_tag="VQ846_001312"
     CDS             complement(9572..10408)
                     /locus_tag="VQ846_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET3075889.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10421..11050)
                     /locus_tag="VQ846_001313"
     CDS             complement(10421..11050)
                     /locus_tag="VQ846_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET3075890.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(11060..11701)
                     /locus_tag="VQ846_001314"
     CDS             complement(11060..11701)
                     /locus_tag="VQ846_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET3075891.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11847..13076)
                     /locus_tag="VQ846_001315"
     CDS             complement(11847..13076)
                     /locus_tag="VQ846_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET3075892.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(13066..14232)
                     /locus_tag="VQ846_001316"
     CDS             complement(13066..14232)
                     /locus_tag="VQ846_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET3075893.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14376..15389
                     /locus_tag="VQ846_001317"
     CDS             14376..15389
                     /locus_tag="VQ846_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET3075894.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPNQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15434..15853)
                     /locus_tag="VQ846_001318"
     CDS             complement(15434..15853)
                     /locus_tag="VQ846_001318"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET3075895.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15864..17270)
                     /gene="atpD"
                     /locus_tag="VQ846_001319"
     CDS             complement(15864..17270)
                     /gene="atpD"
                     /locus_tag="VQ846_001319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET3075896.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17356..18234)
                     /locus_tag="VQ846_001320"
     CDS             complement(17356..18234)
                     /locus_tag="VQ846_001320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET3075897.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(18250..19755)
                     /locus_tag="VQ846_001321"
     CDS             complement(18250..19755)
                     /locus_tag="VQ846_001321"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET3075898.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19770..20306)
                     /locus_tag="VQ846_001322"
     CDS             complement(19770..20306)
                     /locus_tag="VQ846_001322"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET3075899.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(20306..20800)
                     /gene="atpF"
                     /locus_tag="VQ846_001323"
     CDS             complement(20306..20800)
                     /gene="atpF"
                     /locus_tag="VQ846_001323"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET3075900.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20814..21530)
                     /gene="atpB"
                     /locus_tag="VQ846_001324"
     CDS             complement(20814..21530)
                     /gene="atpB"
                     /locus_tag="VQ846_001324"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET3075901.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21565..21765)
                     /locus_tag="VQ846_001325"
     CDS             complement(21565..21765)
                     /locus_tag="VQ846_001325"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET3075902.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         6509 a         4701 c         3988 g         6676 t
ORIGIN      
        1 gatttcttct ttagttgatt tcatattact attgtataat gctttttgat ttgagtctag
       61 tattatactc ttcgaaaatc tcttcaaacc gcgtcagctt cgccttgtcg tatagatgtt
      121 actgacttcg tcagttctat ctacaaccta aaaacagtgt tttgaacagt ctgcagctaa
      181 ctttctataa caaaatgaaa taagaacaag acaaatcaat caagacaatc aaatcgattt
      241 ctaacaatgt tttagaagta gatgtgtact attctagttt caatatatta tagtttgctc
      301 tttgattttc attgagtatt agttcaatat ctaaaaaaag aaccttgcca agcaagattc
      361 ttttagtgtc taacttaaat aattgttctt ctgggaacta aatcgaatta agcttcgatt
      421 tctgtaacca tacctgaacc aacagtacgt ccaccctcac ggatagagaa tgtagtacct
      481 tgttctacgg cgattgggtg aatcaactca acgtcgattg tcacgttatc accaggcatt
      541 accatttcag tacctgctgg aagttcgatt gaacctgtaa cgtcagtagt acggaagtag
      601 aattgtggac ggtagttgtt gaagaatgga gtgtgacgtc caccttcttc tttagtaagg
      661 atgtagactt cacctttgaa tttagtgtgt gggttgattg aacctggttt agcgataact
      721 tgtccacgtt cgatttcatc acgttgaaca ccacgaagaa ggacacctac gttatctcca
      781 gcaagacctt cgtcaagttg tttacggaac atttcaacac cagtaacaac tgctttttga
      841 gtttcttctt tgataccaac gatttcgatt tcgtcgttga ctttaacgat accacggtcg
      901 atacgtcctg aagcaactgt accacgtcca gtaattgaga atacgtcctc gactggaaga
      961 agcaatggtt tgtcagtgtc acgttctggt tctgggatat actcatcaac tgtgttcatc
     1021 aattccataa cgatgtcttc gtatttagag tcaccttcaa gagctttaag tgctgaacct
     1081 tggataactg gaagatcgtc acctgggaag tcgtattctg acaataggtc acggatttcc
     1141 atttcaacca attcaagcaa ttcttcgtcg tcaaccaagt caactttgtt catgaagacg
     1201 ataaggtgtt taacaccaac ctgacgtgaa agaaggatgt gctcacgagt ttgtggcatt
     1261 ggtccgtcag ttgaagctac tacaaggata gctccgtcca tttgagcagc accagtgatc
     1321 atatttttaa cgtagtccgc gtgtcctgga gcgtcgatgt gagcgtagtg acgtttttca
     1381 gtttcgtact caacgtgcgc agtgttgata gtgataccgc gttcgcgttc ttctggagca
     1441 gcatcgatag acgcatagtc tttaggttgg ttaactgatg aaggcaagcg acgtgccaaa
     1501 acagttgtga tagctgcagt tagggtagtt ttaccgtggt caacgtgtcc gatagtacca
     1561 atgttaacgt gtggtttact acgatcgtat ttttcttttg ccatttgagt aaaagcctcc
     1621 aataaaatat attttataga tagacagtag gcaatacagt ctaactttcc ttactatttt
     1681 atcaaatttc agctaaattg caagtgtttt acaacgtttt tgtgaattat tcttggtaaa
     1741 aatttaacta actaatagaa agtactaaat agacttatcc ttcaaggagt tttcagcatt
     1801 atccatccac tcacttgctg aaaatgtaaa cagtctctgg tttatatagt aaaatgaaac
     1861 aagaataggg caaatcgatc aggacaatca aatcgatttc taaaaatgtt ttagaagtag
     1921 aggtgtacta ttctagtttc aatctactat acaacctcaa aactgtgttt tgaacaacct
     1981 gcggctagct tcttagtttg ccctttgatt ttcattgagt ataaaattgc agataaaacg
     2041 ataacaatca taggactcag cagagtcaat gtaggtcgaa gaatagcccc cctaatcgct
     2101 tattttatag caaaatgaaa tagaaactga acattttgtt gaggaaggta aatcaattcc
     2161 tcacaatgtt tgagaaagaa gggaggacta ttccaacttc aacccactat taatatataa
     2221 tagaatgaaa actgcacaat ttgattggag aattcaaacc aatttctaac aatgttttag
     2281 aaacaagggt ctattatttc aactgcaacc tactatagcc cattgaagct ggaatagccc
     2341 actgagactt ctaaaccact gctggagagg agtttgttta gagtttcttt catttcccta
     2401 aaaaatcaaa aagagagaac cagtctgatc ctctcttact tatagatggt gctcactcaa
     2461 atgttaaagg agctatttct tagagataaa ggaatttgaa tacagctact gccgcaattg
     2521 ctgctgcgat aggtgctact actggtaccc aagaatacca ccattttgaa tcgcctttat
     2581 gctcaccaag aactgatttt ggaaggaaag catgaaggag acgtggtccc aagtcacggg
     2641 ctgggttcaa ggcgggtcct gtaggtcctc caagtgatgt taccaaagcc ataacgagga
     2701 atccaagtgc caagtgagcc acagaaagtc ctgaagcagt gtgtggagcc acctgtgctt
     2761 taatagccaa gtcagaaaaa tcaactgttt gtcctgcttc tattgccttt tgtttcatga
     2821 attgaagcac ttcagcacca aagaagtttt tagtcaaacc aagagctgca aagaacaaaa
     2881 caaatgaacc aacaaactca ttaatcaaac cattgacagt tgctgcatag cgactttctt
     2941 ttgtaccatg gtcaatactt gaaatagttg agaaagttcc caagatgtta tttggttttt
     3001 cagttttcaa gtagaatgga cggtatgttg ccacaactaa ggcttggcca aagatagccc
     3061 ccaagacttg cgcgataatg taaggtacca cttgtgccca agggaaaaga ccgctaactg
     3121 caagccctag agtgaaagca gggttgatgt gattcccaga tacgttacca aacatcaagg
     3181 ctgggatcat aacccccata ccataaccaa cagcgatgac gatccagcca ctttggtgac
     3241 ctttcgtacc tttaagttca acgttggcaa ctgcaccatt cccaagaatg atcaaaatgg
     3301 cagttcccaa aaattcagtg gcatacttca gtgcccatgt gaaatccatt tgctagattc
     3361 tccttaaaat tttttagata ttccttattt tatcaatttt taccctattt agcaactgat
     3421 ttctggaaat atttttaaaa aaataggatt catagtttcc tagttcaacg tacttgtaag
     3481 cgtttttatt cttcacgaaa gacctcattc tcaaaaccag gaaaaagccc acatctgtga
     3541 tcttttcctt gttcctagat tctaaaatct attatttttc atcttctttt ttcttgaaga
     3601 atcctaatcc tagaccagct aacaaggtca tgataccagc actagccaag gtagcattag
     3661 cttctgtacc tgtgtttggc aattcctctc tcttacctgt ctcataagtc ggaacttctg
     3721 ggtctggatt cactggagtt tcagtttttg gagtacctgg ttctggagtt ggtttatctg
     3781 gtgttggagt ctcttgattt ggtagttctg ggtttggtgt cggaacactt ggatgctctg
     3841 gatttggttg tgggattggt ttttctggag tcggttcacc tggagtacct ggttcctgag
     3901 gacttggaat ttctggtttt tctggagttg gtttatctgg tgttgataaa cggtcatacc
     3961 ttaccgttat ttctttatca ctagagtctg acgtaacttc ttgtgattca actgttggaa
     4021 tatctggatc tttgtacttg tcaatcttac cagatataac ctcgtcccag tttcctgttg
     4081 tccattcacc gtaggttaca actcccgtga ccttgttctc agtttttgta cggcttaagg
     4141 ttacaggttg aacaacatct tcttttacat tttggttcgt aactttatca acgtaatgaa
     4201 tgatacgcgt tatagtcttc gtctcagtag aggttgctgt tttgggaacc actgtttcct
     4261 caacattctc acggtagtaa tagtcaactg ttgcaccgtc ttctggtacg catttgcagg
     4321 agttgctacc aaggtgtatg ttgttttcct tgtgataact cggtcttctt tgtcctcagt
     4381 tgttgttttc ccttcaatag tttttgattc tgtggtatac tcagaaccta tcgctaaatc
     4441 agcttttata acagactctg ccaacttctc ttggctacct tctttatagt aattggctgt
     4501 tactgtagca gtggttggcg cttcgctttc tctataaact aaggtcactg ttctaccttc
     4561 gcttacaata ttcccagtta aacttgcaga atttgtatct gcttctttaa aagtataata
     4621 ttttccgtca gtagtagtca tgctactgag ttttttatct gtgacattgt agctggtacc
     4681 aatcagttgt tttttattgg taatgtaggt tccgtcactt tctttttctc caattccagt
     4741 atcatttttc aatgatagca aacgcccttg ttcatcaaca tagcgaactt tcacattttc
     4801 taagattagt tctgccaatt ctgaggtttt tttctttttc ttgatttctt cggttatttt
     4861 ccctttctct tcttcgggaa tatttagttt tggaatgatt ttttcaacaa cggttcgtga
     4921 tggttccaca gtatcttgga tgactgaaaa gtcagctaga attgggagat tatactgaac
     4981 acggtgactt tgagtgttta ctcctactct ttcattattc tctgaaaata cttgtacggt
     5041 ataagaaaca acatcttttc ctaatagaac atccccagta gagaaatagc cgccttttcc
     5101 tagtttgcta tctccagagc ccacttcttt cctaatctta tcagatagtt ttttaccagt
     5161 cagtacattc gttcgcacaa tccctttgtc tacccctaca aagtgggaga actttttgaa
     5221 ctcttcagaa ccagatctaa cccaaccatt attaagggca tttgcttttg tatttgtatt
     5281 ctctctcaaa agtttggcga ttagaattat attcatcggc acttagagtt gctgctatat
     5341 ctgactcttg aataccaact tccttactac catttctagc ggcagtatat gtgtatttat
     5401 ctgtttctac ttctagctga ttatccttaa catgataggt aattttctcc tggtataaga
     5461 gtcttcagta gtttatatac tctgtcatca tctttagctt tagaagcagc tgagtttagc
     5521 caagttgggg tattaggagt atctttatca acctgtttca agacattatt caagtcattt
     5581 gctagcctat tggttgtcct cactgtgaaa gttttcttcc agtctgaacc acttttatta
     5641 ggcttagata tgctcatgaa atcctggtca acattcaaca caatatcgcc ttgtttttta
     5701 gcatcttccg ttgcttctgc ttcaaattga tcatggagtt tttgttgctc tggtgtatag
     5761 tttttttatt tacacgattt aatgtactat tttttatttt agtattaaga actccattaa
     5821 tacgattttt aaattctgct tcaaaaaatc ccttaatttg ttggtgcgca agttcaacat
     5881 tcaaaccttt cttatacaag tcttcattct gatacaagtc ttcattctga acgatttcgg
     5941 ctacacggtg aatggtttga ttgtataccg tcttctctta accagcagta tccttagttt
     6001 taatgactac tcgataggtt cctttagaac cgttatagtc atctagtttt aaaaaagcat
     6061 tccaaacatt caaaaatagt tggaacttcc cttaataaat ccgtcacttt ctttttcaag
     6121 atagcccaag cttgctcaat aggattcaaa tccggtgaat aaggtggtag aggtaagaaa
     6181 aagtgtttat cttggatgca gagttcatcc aaaatgttct tgggatgaaa actagcattg
     6241 cccatgacaa taacatgagg tgtcttcaaa gcaggtagga gttgcgtttt gaaccactcc
     6301 acaaagaaat cgcttgtcat gcttttcttg taaatcatgg gagctataaa ctctccgtct
     6361 acttgtcctg caacaattga agttcgctca aaacgacgtc cgctaatctt ttcatagact
     6421 ttctcccctc taggagcccg tgcataagga cgatagaggt agcggtcgat tcctgtttca
     6481 tcaatatata cgattggtaa atcttttagg ttatccaaaa tatcaagaaa ctcagcgact
     6541 ttctctggct cttgttcctt aaagctcgtc atcttttttt aaagtgacct taatatactt
     6601 taaagctgcc catacggagg gcagagcaca atcaaaatgt gccgcaattt cccgtaaaaa
     6661 agcatctgga tgagcctcta caaaggcttt caattcctct aaaggaatct ttcggttttt
     6721 gacgactcgc tttttcctct ctatgtgtcc ttgttcacat ctttcttttc ccacgtgaag
     6781 agagttctga cgccaacacc aaaaaacttg gctgcctcga catggctgtg cccctctttg
     6841 atggaatcta atgctcgctg tttaaaatca gtactatatg ccatagcttt attataatat
     6901 gcttgtttaa aactaagcaa ctatatcaat gttttgttat tagaagactt acttttttta
     6961 ctgaaatcga atttttccta acctcaacat catataaccc caaaattcaa tctgacctgg
     7021 tttgaagaga tgttcgaagc agataaggtt ttactaaaac taaaaaaaat gatataatca
     7081 aactgattaa atgttttaaa aataaaggag aatttgaatg tcttaccaag aaaattacca
     7141 gaaatgggtt gattttgtgg agcttcctga ctaccttcgt caagatttgg aaaatatgga
     7201 cgaaaaaact aaggaagatg ccttctatac aaatcttgaa tttggtactg caggtatgcg
     7261 tggcttggtt ggtgctggta caaaccgtat caacatctac gttgttcgcc aagctactga
     7321 aggattggct cgtttgattg agtcaaaagg tggaaacgag aaagaacgcg gtgtagcaat
     7381 tgcctacgat agccgtcact tctcacctga gtttgccttt gaatctgcgg cagttcttgc
     7441 taaacacggc atcaaatctt acgtatttga aagccttcgt ccaactccag aactatcatt
     7501 tgcagttcgt catctcaact gtttcgcagg tatcatggtc acagccagcc acaaccctgc
     7561 tccatttaac ggttacaagg tttacggtga agacggtgga caaatgcctc cacacgatgc
     7621 ggacgctttg actacttata tccgtgcaat cgaaaaccca tttgcagttg aagttgctga
     7681 tgtggaaact gaaaaagctt ctggcttgat tgaagttatc ggcgaagctg ttgacgtaga
     7741 ataccttaaa gaggttaagg acgtaaacat caacccagcc ttgattgaag aatttggtaa
     7801 agacatgaag attgtctaca caccacttca tggtactggt gaaatgttgg ctcgtcgtgc
     7861 tcttgcccaa gcaggatttg actctgttca agttgttgaa gcgcaagcaa ctgctgaccc
     7921 agacttctca actgtaacat ctccaaaccc agaaagccaa gcagcctttg cccttgctga
     7981 agaacttggt cgtcaagttg gtgcagatgt tcttgtcgca actgacccag acgctgaccg
     8041 tgttggtgtt gaagttcttc aaaaagatgg tagctacctc aacctttcag gtaaccaaat
     8101 cggtgctatc atggctaaat acatcttgga agctcacaaa aacgctggaa ctcttcctga
     8161 aaatgccgct ctctgcaaat ctatcgtttc aactgacttg gtaacgaaga ttgctgaaag
     8221 ctacggcgca actatgttca acgtcttgac aggtttcaaa tttatcgctg aaaaaatcca
     8281 agaattcgaa gaaaaacaca accacactta catgatggga tttgaagaaa gcttcggtta
     8341 cttgattaaa ccatttgtac gtgataaaga tgccatccaa gccgttcttg tcgttgctga
     8401 acttgctgcc tactaccgtt ctcgtggttt gacacttgct gacggtatcg aagaaatcta
     8461 taaagaatat ggctactacg cagaaaaaac aatctctgtt actctttcag gtgtcgatgg
     8521 tgctgaacaa atcaaagcga ttatggctaa attccgcaac aatgctccaa cagaatggaa
     8581 cgcaacagct atcactgtcg tagaagactt caaggcacaa actgctactg ttgctgacgg
     8641 tactgttaca aacttgacaa ctcctccaag tgatgtgttg aaatacacac ttgctgacgg
     8701 ttcatggatt gccgttcgcc cttcaggtac agaaccaaaa atcaagttct acattgcagt
     8761 tgtaggggaa accaacgaag aatcacaagc taagattgct aacatcgaag cagaaatcaa
     8821 tgcatttgta aaataaagac ctataaaaga tgagaccaac caacctcatc ttttttcgta
     8881 tcaaatctaa gcaattttag aaactttatg gcatactaga cttatcaatg aaagcgaggt
     8941 aatctcatgg aacaattttt agataacatc aaagaccttg aagtcactac agttgtgcgt
     9001 gcgcaagaag ctcttgataa aaaagaaact gcaaccttct ttatcggtcg caaaacttgc
     9061 ccttactgcc gtaaattttc aggtacattg tcaggtgtcg tagctgaaac caaagctcac
     9121 atttacttca tcaatagtga agaaccaagc caactcaatg atttgcaagc attccgctca
     9181 cgctatggaa tcccaactgt accaggtttt gttcatatta cagatggaca aatcaacgtc
     9241 cgttgcgact cttcaatgtc agcacaagaa atcaaagatt tcgcaggatt gtaaaaaatc
     9301 agctaacaag tcttaaaatc acaaaaacgc ataatgtaag gtgcaaaaac cttgatatta
     9361 tgcgttttat tgttcctaaa acagttacat cagcatttca ttacagaaca ttttaatttt
     9421 ggaagtcaaa tcaattttta acaatgtttt agaaatcaca gtgggctatt ccatattcaa
     9481 ttatccagat tcaatcgcta ttatcgtttt cctagattac aaaaaaacta cgttttgcta
     9541 atcaaaacgc agtttcagcc actattttat cttactgtcc ttcttttact tctttggttg
     9601 ctacatcttc tccaaaccat ttttgactga tttcttggaa cttgccgtcc ttgtaaagac
     9661 tagaaaaagc ttcatttatc ttcttaacca agtttgtatc ttccttacgg gctccaaccg
     9721 caaaagcttc tgtttctagt ccaactgtaa agacattata atcgtttaaa acaccttctg
     9781 cttctaaata ataatttgca tagacacggt caatcaatag accatcaatt cgatcgtttt
     9841 tcaaatcaat caaggcttca ttaaaggttt ggtattgatt cgcttcctta ttagcgacaa
     9901 tattcttcaa aatttctgga tttgcttcaa agtccgcata accagatgaa ccagcttgag
     9961 ctcctaatgt ctttccagtc atatcctttg cagtcgtgat accagatgat ttcttcgtaa
    10021 ccaatacctg ctcattcttc atatatgagt tactgaaagc caccttttca cggcgttcgt
    10081 ctgtagcgga atagccattc caaatcagat caatcgttcc ttttgtcaat tcagcttctt
    10141 tcaaatccca atcaatcggt tgccaattta ccgtgattcc gtatttttca aaaacagctg
    10201 tagctaaatc aatatcaaat cctgcataag aaccatcttt ctgagcaaat cccattggaa
    10261 caaaagtact atcaaatcca atagtaatag acttgttaga ctggtacttt gaccaattat
    10321 ctccactagt ttcgctagaa tttttcccac aagctactaa gaacaaagca gtcatcagac
    10381 tgactaatac aagcatccat tttttcatcc atttttcctc ctatttaggt tctactttca
    10441 ataatacatc tgcgatattt tcagcaaact gcaaatcatg ggtaaccaca atctgggtca
    10501 tcccaagttc cctattttgc aagattagct tctccacttc caaacgtaat tctggatcca
    10561 gggcagaagt tggttcatcg tagccaatga tttctgggtc aatcatcata gcacgcgcca
    10621 aagccacccg ctgcttttgc ccaccagata gtgagaaagg ataggcctct gcgtgtcctc
    10681 ctagtcctaa ctgttccaag agtccactcg ccttcttctc agcctcttcc tgcttcattc
    10741 ccatggtctt cacaggcgat aaagtcaaat tttccagaac tgatagatga ggaaatagtt
    10801 gaaaatcttg gaagacaaat cccagtaggt tgcgcttctg caattcatct agctctaaag
    10861 gttgtccatt ataaaagatt tgccctgaat caatggtttc aagacctgca agcatacgta
    10921 aaagagttgt cttacctcca ccagaaggtc caacgatagc caggatttgc ttttcaggaa
    10981 tacttagact gaaatcagac aggatttgtt tgtctccaaa gactttattg atatttcgta
    11041 attctaacat agctgccccc tatctataat aactgtactt tttctcaact tttttggaaa
    11101 taattgtcac aatcccaatt aaaatcaagt aaatggctcc tgccaagaac ataggaacca
    11161 gactagcatc gcggttagca gctgttcgac tagccaagat aaggtctgaa attccgagag
    11221 catagaccaa agaagtatcc ttgaccaaac tcataacttc attaaagaca ctaggaagaa
    11281 cgatcttggt cacttggggc aagataatat agcgcactct gtcaaaaggg ctaaacttca
    11341 agaccttggc agcctcatac tgtcctcttg gaatagtgtc aattccccca cggaaaattt
    11401 ctgcaaagta agctgcatag ttgagaacaa aggcaataat agctgcagga aggcgatcta
    11461 aacgaatccc aatacttggg agcacataat agataaaaat cagttgcaag agtaaggggg
    11521 ttcctcgcat aacccagatg taaatgttaa tcagataatg gaggggcttc caatggactt
    11581 gcaaggcaaa ggcaatcaaa acgcccaagg gaatcgaaaa tagcaagacc agtgcaaaga
    11641 cctgtacagt cgtgctcgca ccattcagta aactcggtaa tatctcaaat aaataagaca
    11701 tactgcacct cctaaaagta attgttccta ttatagcatg aataaacaag attaccaagt
    11761 atttttgtaa aaagattcta ccatatacaa ataaaaaccg tagctatcaa aagagataaa
    11821 ctacagttct atatgtttaa acattcttac aatctctcaa acagttcttg catttgcaca
    11881 tcatctggaa ccagttttaa gtaagtgtga gcatggactt ttgcttcttc aaaatgtccc
    11941 aattcacgca agagatagat atagtgttcc agaaattctg gattgtcctt caaatctcct
    12001 gtcaactctt gataatgctc ataagcagta tccaaatcgt ccatttcttg ataagaacga
    12061 gcaatcatcc acttggtcaa aagattttct ggctcctcac tctgcaagtc tagaatatcc
    12121 tcataacgct cctgctccag ataaatagtg gctaaacgaa gcaagatttc ttctgtatcc
    12181 tcagcgtctt cttttgcagt aaggagataa ttttctgcac cactagcatc atgcaattca
    12241 taagaaaatt gtgaagcagc tagcaagagg cgagtttcaa agggattttt ctctaatcct
    12301 tgcttagcga tacgcagggc ttcttgaact tgatgttcct tatgtaaagc ctgactgtac
    12361 ccatactcat agccttcaaa gtcaggagaa atggtatcaa gctgcttaaa gtagagggtg
    12421 gctttttgat attcttcttg atcaaaataa agactggcca actcaaaagc tgttaagtca
    12481 tcgtattcta actccagggc tttttctaaa aactcagtag ccgtttcaaa tttccctaac
    12541 tgagcatagg caaagccaat tcgttgatag gtggaaatgc ccgtttgctc ataaatcgag
    12601 cgattatcta actgggcata ggcttgaata gccgcttggt aattttccaa ctcactatcc
    12661 aactctgcca aacccaatat caagagagaa tcctctgagt aggtcaaggc ctccaataat
    12721 ttctcacgtg ccacatctgt caaaccttcc agctggtaaa ggtctgcctt cagagccaaa
    12781 gacgagacat accagtcact gtcagcttgg atttcctcaa gataggtaaa ggcttcttct
    12841 atttgaccat cctcgctagc aattgcagct agattaagat gaacctctgg aaaatcctct
    12901 acaattttca ggtaaatttc cttggcctga ggatagaaac caatcccttc aagataagtt
    12961 gccaactcat acagaagatc acttgaatca ttttctaaag ctttggcgaa ataatgctca
    13021 gccttagtta aatcttgctc ctccaaagcc tgtaacatct gttgactatt gttcactctt
    13081 gacttcctca ccctctaatt catatagacc actgactacc ttataccatt cgaaaatggc
    13141 tgaaatgaca accttagcag aggcataaac cggaatacca agtaaaactc cccagatacc
    13201 aaacatagat cctgaagtta acaaaacaaa gagaacatta ataggatgga tgtttaattg
    13261 acttcccaaa atcaatggag agacaaaacg gccttcaata gtttgttcta caataaagac
    13321 aatcactact ttcaaaagca tgactggacc agcaatcaaa cccaatacta gggcaggaag
    13381 catggctaga aagctaccaa gataagggac cagatttaaa ataccagcag taacccccag
    13441 cgtaaccgca tagcgtagac caataatctt gaagaagatg ataaacatta ctgctacaat
    13501 aatagccact gtcacttgcc ctcgaacata gttggacaac tgttgattca catctgatag
    13561 aacttgtcca acaggttcct tcaattttct tggaatgaat tgggtcaaat agttacgcaa
    13621 gcctttccca tcacgcaaga gataaaagag catgaaagga acgataatca aggcaacaat
    13681 cacttgagaa gctccgctaa taaaggcact cacccagttg actgcctgag atgaaacctt
    13741 acttgccaaa actgtagcct ggctagaaaa attggtcaaa acttgctcta attgaggtct
    13801 gaaatcatct ggcaggtgct gggctaccaa tccattaaca atcctatcta tatcttctaa
    13861 gtaaacagga acgtttcttg caaaggtcaa aacctgacgt tgcagatttg gaatggcgac
    13921 tgccaagccc caaatgataa agagagcgat gataacaaag acaatagtga tagctataac
    13981 acgattaacc ttatgcttct ccatccaatc aacaatagga ttcaacaaat aatataacaa
    14041 accagacaaa atgactggca acatcacaac tgctaaaaag tctaaaacag gtgaaaatag
    14101 aaaactaatc ttacttaaaa taaaaagatt cagtcccaat aataaggtta ctaaaaatac
    14161 cgtaattgcc ttgttatcta aaaaccactt gaaaaaccaa gataggctaa aatgtttctc
    14221 tttttgctcc ataaatactt cctttctatt gtcctctcat tataccattt ttgaaacaaa
    14281 ataactcttt tttaaagtgc ttacatctaa ttggatatct ttaatttact tgtttttgtg
    14341 atataatagt cttatcttta cacagaggta tagtaatgaa agaaactgtt tattttggaa
    14401 cttatacacg tcgtacttct caagggattt acaaggcaga ctttgataca gaaactggtc
    14461 agctttcaaa tctagaactt tttgcagctg agccaagtcc aacctacctt gcctttgacc
    14521 agcaccaaca tttatacact gttggtagcc aagacgataa ggggggaatt gcagcctatc
    14581 aaactgacgg gactgtgtta aatcatgttg ttgaagaagg agctccccac tgttatgttg
    14641 ctgtcgatga aaagcgtgat ttggtttacg cagccaacta tcacaaggga caagtccttg
    14701 tttataaacg ccaggaagat ggtagtcttc tacttagtga tatggatcaa cacagtggcc
    14761 aaggtccaca tgaaaatcaa gcttcccccc atgttcacta tacagattta acacctgacc
    14821 actatctagt gacctgcgac ttgggaactg accaagtcat cacctatgac ctcgatcaag
    14881 aaggaaaatt atctaagctc tatacctatc acagcaagcc aggagcaggc tcacgccata
    14941 tcattttcca taaccactat aaaatcgctt atctcatttg tgaactcaat agtactatcg
    15001 aagttttaat ctacgatggc gttggcgaat ttgtacgtat gcaggttatt tcaactttac
    15061 cagaagctta cgaaggcttt aatggaaccg ctgctattca tctctctaaa gacggtaaat
    15121 acctctacgc ttctaaccgt ggccatgatt ctatcgcagt atataccatc cttgcggacg
    15181 gtagcttaga gttattagaa atcgttccaa cgcatggtca gactccacgt gattttgatt
    15241 tgacacccaa ccaaaaattt ctcattgttg tccatcaaga ctctgacaat gcaactgtct
    15301 ttaaacgtaa ttgtgacaat ggtcgtctag cagaactctc caacgacttc catgttcccg
    15361 aagcagtctg catccgtttt gctccttaaa acaccataaa aaatgaactt ggtattttca
    15421 agttcatttt ttcttatagt ctatttccga cattaatacg gttaatggca cgttgcaaag
    15481 caatcttagc acgacgttct tggtcaatca aatgtttgtc ttgtgcttct tcaattgcac
    15541 gttctgcacg aagtttggca cgttctgcac gactgatatc gatatcacga gcacgttctg
    15601 cagagtcagc gacgattgtg atcatatcat tggcaatttc aataacgcca ccgtttactg
    15661 caatccagtt cacgtgatct ttatcatcga tacgttttac ctttacttca tcaactgcta
    15721 aaaccgcaat catattttca tgtcgtggca agatccccat ctcaccatcc agagttcgaa
    15781 ccgatacata gctggcatgg tgatcataga cgagaccatc tggtgtcacg atctggacag
    15841 ttaactgagc cataggtcac ctcttaaaat cccatttttt cagcttttgc aatcacatct
    15901 tcgatagaac ctacaccacg gaaggcatct tctggcaagt ggtcgtattt accatcaagg
    15961 atttccttaa agccacgtac agtttcagca actggaacat aagaacctgg ctgaccagta
    16021 aattgttccg caacgttgaa gttttgtgac aagaagaact ggatacgacg ggcgcgagca
    16081 accaaggtct tttcttcatc agaaagctca tccataccaa ggatagcaat gatatcttgc
    16141 aattcatggt aacgttgaag gacacgtttt acttcagcag caactgcata gtgctcttct
    16201 ccaacgattt caggtgccaa ggcacgtgag cttgaagcaa gtgggtcaac ggctgggtag
    16261 atacccaatt gtaccaactt acgttccaag tttgttgttg aatccaagtg agcgaaggct
    16321 gttgctggcg ctgggtcagt atagtcatcc gctggcacat agatagcctg gatagaggtt
    16381 acagaaccct tcttggttga tgtgatacgt tcttgcaatt gacccatttc cgtagcaagt
    16441 gttggttggt aaccaacggc tgatggcata cgacccaaaa gggcagatac ttctgaacca
    16501 gcctgagtga aacggaagat attatcgata aagagaagca cgtcttggcc ttccacatca
    16561 cggaagtatt cagcgattgt caaaccagta agggcaacac gcatacgtgc tcctggtggc
    16621 tcattcatct gaccaaagac catggctgtt ttctcgataa cgcctgattc tttcatttcc
    16681 cagtaaaggt cattcccctc acgagtacgt tccccaacac cagtaaatac tgaaatacca
    16741 ccgtgctctt gggcaatgtt gtgaatcaat tcttggatta agacggtttt accaactccg
    16801 gcaccaccga aaagtccaac tttaccacct ttaaggtaag gggcaagaag gtcaataacc
    16861 ttgatccctg tttcaaggat ttcagaagag gtagacaact catcaaaagt tggagctttt
    16921 ttatgaattg gctgacgctc tgcgtcttct gtaaaaggag cttccaaatc aatggtatct
    16981 cccaaaacgt tgaagacacg tcccaaagtt tctttaccta ctggtacaga gattggacga
    17041 cccgtgtcca atacttccat tccacgagtc aacccatctg ttgattccat ggcgatagta
    17101 cgaaccatac catctcctaa ctccaaggct acttcaagga cgatttttgt ttttctttcg
    17161 tcatttttgt agacgacaag tgcattgtta atctcaggaa gtttttcccc tgctgcaaac
    17221 aaaacgtcta caacgggacc gataacctga gcaattttac ctgaactcat ctccttctcc
    17281 tattctatat aagatactgt cggttcctag ctcaactagg tcctaagttc attttcatac
    17341 gagctggact agagcctatt ctaaggcact agcacctgct acgatttctg taatttcttg
    17401 tgtaatcgcc gcctgtctgg cacggttata ctgaattgtc aaatcattga tgactttctt
    17461 agcattatct gtcgctgttt gcatggctgt catacccgca gcattctcag ctgtcttggc
    17521 atcgataatg gcaccgtaaa tcatactttc tgcaaactga ggcaacaact gctccagaat
    17581 ttcttctcgg ctggtttcca attcaaaagt caagctgtac tcttcatccg cttcatttgg
    17641 atccaagtca acaatcggaa gcatttgttc cacacgcatt tgactggtta gcgtattgac
    17701 atggtggttg tagcaaacat aaagctcatc aaagagttca ttttggtaca tttcaacagt
    17761 ttttgaaata atcttacgaa cttgatcaaa gctaggttgg tctgccaagc cacgtaattc
    17821 ataaagtggt tgaataccgc gagccttaaa gaaatcagct cccatcccac cgatacagat
    17881 catttcaaaa cctttaccgt ctgggtggta ttcttctttc aactccataa cagctttcaa
    17941 aatagaggaa ttataacctc caaccaaacc gcggtctgaa gtgataacga tatagcctgt
    18001 cttcttcaca gaacggctaa tcaacatcgg attagttgaa gcaccagctc cattaccatg
    18061 aaggatatct gtcaaaagtt tacgcacttt ctgagcgtaa acttggaagt tgcgagcagc
    18121 ttcttcagaa cgacctagct tagcagccga taccatttgc atggcattag tgatttgact
    18181 cgtatttttt gttgaggcga tttttgtttt aatatcattt agagatactg ccatctgaca
    18241 cctctattct tattggaagc tagattgatt gagaaactct gtaatcgcag catccaagac
    18301 tgcttcttct ggcaagtctt ttgtatcacg aatggtttcc aaaatctctg gatgttgagc
    18361 atcaaagaag gtatggaact cttcctcgaa acgaacaata tcatctactg gaacagtatc
    18421 caagaaacca tgtgtcaaag cataaagaat ggttacttgt ttctcaacag gtaatggttt
    18481 gtgaacaggt tgtttcaaga cctcaacggt acgacgtcca cggttcaact tagcctgtgt
    18541 tgctgcgtcc aagtcagaac caaacttagt aaaggcttcc aactcacggt atgaagcaag
    18601 gtcgatacga agtgtaccag caaccttctt catggctttg atttgtgcag aaccacctac
    18661 acgagataca gatgaacccg catcgatggc tggacgaata cctgcattga agaggccatc
    18721 gccaaggaag atttgtccat cagtgataga aatcacgttg gttgcgatat aggctgagat
    18781 atctcctgct tgtgtctcga taaatggtag ggctgtaatt gatccaccac caagttcatc
    18841 agaaacttta gctgagcgct caagcaaacg gctgtggaga tagaaaacat cccctgggaa
    18901 ggcttcacga cctggaggac gacgaagcaa gagcgatagt tcacgataag ctaccgcttg
    18961 ttttgataga tcatcgtata caatcaaaac atgcttacct tgatacataa attcttccgc
    19021 catagcaacc ccagtataag gagctaggaa gagcaatgga gatggttgtg aagcagaggc
    19081 tgtcacaacg attgtgtagt ccaaggcacc gtactgacga agtgtttcta cttgcgtacg
    19141 aactgttgat tctttttgtc caatcgcgac gtagatacag atcatatctt gatctttttg
    19201 gttcaagatt gtatcaatcg caatggttgt tttccctgtc tgacggtcac cgataatcaa
    19261 ctcacgttga ccacgaccaa tcggtacaag ggcgtcaata gctttcaaac cagtttgcaa
    19321 tggttctgaa acagacttac gttgcataac accaggagct ggtgcttcta ctggacgagt
    19381 tttatcagtg tggatttctc caagaccgtc aactggacga ccaagcggat ccacaacacg
    19441 accaatcaga ctttcaccta cagggacttc catgattttc cctgtacggc ggattgtatc
    19501 gccttcacgg atatctgtaa agtcacctag gatgataata ccaacgtctg ttgactccaa
    19561 gttttgagcc ataccataag agccgttttc aaaattcaac aactctccac tcatgacatt
    19621 ttcaaggcca tgagcacgcg cgataccgtc cccgatatag gttacaacac ctgtttcagt
    19681 cacatcaaaa ttgggtttga aattttcaat ttgttgctta attaaagcgc tgatttcttg
    19741 tgcgttaatt gccaaaagaa caccactttc tatttcaaat tttctttaac aactttaagt
    19801 tgttgtttaa tactcacatc aattgtcttg tgattggcaa aaatgacaaa accaccaatg
    19861 agactttcat cgatttgttc ttttacactc cttactttca gagacatttt tttctcaatc
    19921 aaagggagca aacgagtctt ctgttcatca gttagaggat gagcagacgt aatcgtcact
    19981 tcaaatcgat ttgtttcttt ttcaagtcgg ttcaagcaat ctacaagcac atcataaaaa
    20041 agatttgctc tgtgattgta ggccagaacc tggataaagt tttgtaataa aggcgacaca
    20101 gaatcttgga aaaaagcaat tgttttttcc ttatccgact cgtctactgc cacttgtttt
    20161 aaaaaagaag gcagacctgt tttttcaaca acttgcttga tttgagtcaa gtctgaaaag
    20221 atacggtctt cttctccttt ttcaagtacc aattggacaa aaggcatgct gtatttttca
    20281 attaccttta ctgttttctt gtccattaag cttctcctag ctgatcgata tactgatcaa
    20341 tgagtgcttt atgggcatga ctgtcaaggt tttgtgagat gattttacca gctaagctga
    20401 tggtcaaatc tgcgacctca cccttaacac tctgtaaagc ttctacttta ttttgagcaa
    20461 tttcttgatt ggctttttct tttaagtgtc ctgcttctag tttagcatct gctaagatat
    20521 tagccttact ttgctcagct gtttcctttg cattttcaat gattgtctta gcttctttac
    20581 ggctaccagc caattcatct tcgcgttttt gagccaatac ttctgctttt tgacgggctt
    20641 cttcagctct gtcaatatct gaagcaattt tttcagctct ttcttcgaaa atgcctgtaa
    20701 tattagacca tgcaaatttt ttaatcaaga ctagcaaaag aataaaagag ccagtgatta
    20761 aaataaaatt accaattaat tcacctactg ttacgtgcat cagttactcc tttctattcc
    20821 tcttcaatat taatcttatt ccctaaatac acagatgtca aaagagtaaa aacataagct
    20881 tggatgcagg aaataaagac agaaaatgca gtccaagcca aattagctcc aaaggctact
    20941 ggataccaat aaatagcttg gtgggaaaga agaagtaaca aactcgtcat gacctctcct
    21001 gcaaagatat tcccaaaaat ccgcaaagcc aaagataaga agtttgtaaa ttcttccaag
    21061 atattcatcg gtatgacaaa aacaggactc ataaaagatt ttatactttt tttaaatcca
    21121 cgacgacgaa cgctttctat atgtgtcaac aaaatgacaa gaaaagataa ggttaagtca
    21181 tactgtaaat tagcggttgg cgaactccac cagttagtcc catcgatcgt ttgaagcttt
    21241 gtcattaagc caaggttatt ggcaatcacc atgaaaagga ataaacaaag gaaaaagagt
    21301 gagtaatctt tcatgtagcg cgaaccaatg ttaggttctg taaatccaat aacaaagtca
    21361 tagacatact caagtacatt ttgctttcct ttgggtttca aggtcatatt gcggcttgcc
    21421 caataaataa agacaaaaat aactcccaca atcaacaaag tcatggctaa catagttaga
    21481 ttgaagataa caggaccaat agagatgatt ggattaatac tttcttccat ctaaaatcct
    21541 cccttttctt ctcgttccat gtatttattt gataataaat gagaagacaa gagttacaaa
    21601 gaaagttcct tcaataaagg caacacctaa aaacatcaaa ctacgaaact cagaaagcat
    21661 atctggttgg cgtgctactg atttaaacag tccattcatc aataaacctt caccgacaga
    21721 tacgcccata caggcaatac ataagcctaa aaatgttaaa ttcataacaa attctccttt
    21781 aaaataaaat ttacttactt agtgtagtcc taaaatttaa atttgtcaac ttttaaccgc
    21841 tattggaagc gtttaataaa aattatttcc attt
//
LOCUS       DAVBNO010000032        21565 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVBNO010000032 DAVBNO010000000
VERSION     DAVBNO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.032"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            1..1024
                     /locus_tag="VQ846_001326"
                     /pseudo
     CDS             1..1024
                     /locus_tag="VQ846_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(1417..3144)
                     /gene="ezrA"
                     /locus_tag="VQ846_001327"
     CDS             complement(1417..3144)
                     /gene="ezrA"
                     /locus_tag="VQ846_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET3075903.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(3226..5172)
                     /gene="gyrB"
                     /locus_tag="VQ846_001328"
     CDS             complement(3226..5172)
                     /gene="gyrB"
                     /locus_tag="VQ846_001328"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET3075904.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(5187..5750)
                     /locus_tag="VQ846_001329"
     CDS             complement(5187..5750)
                     /locus_tag="VQ846_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET3075905.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(5835..6389)
                     /locus_tag="VQ846_001330"
     CDS             complement(5835..6389)
                     /locus_tag="VQ846_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET3075906.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(6400..7623)
                     /locus_tag="VQ846_001331"
     CDS             complement(6400..7623)
                     /locus_tag="VQ846_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET3075907.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(7660..10110)
                     /locus_tag="VQ846_001332"
     CDS             complement(7660..10110)
                     /locus_tag="VQ846_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET3075908.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKMAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQNLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEVKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(10310..11239)
                     /locus_tag="VQ846_001333"
     CDS             complement(10310..11239)
                     /locus_tag="VQ846_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET3075909.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            11379..11666
                     /locus_tag="VQ846_001334"
     CDS             11379..11666
                     /locus_tag="VQ846_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET3075910.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(11700..13034)
                     /gene="ciaH"
                     /locus_tag="VQ846_001335"
     CDS             complement(11700..13034)
                     /gene="ciaH"
                     /locus_tag="VQ846_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET3075911.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(13024..13698)
                     /gene="ciaR"
                     /locus_tag="VQ846_001336"
     CDS             complement(13024..13698)
                     /gene="ciaR"
                     /locus_tag="VQ846_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET3075912.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(13807..16353)
                     /locus_tag="VQ846_001337"
     CDS             complement(13807..16353)
                     /locus_tag="VQ846_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET3075913.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(16476..16916)
                     /locus_tag="VQ846_001338"
     CDS             complement(16476..16916)
                     /locus_tag="VQ846_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET3075914.1"
                     /translation="MTPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(16891..17760)
                     /locus_tag="VQ846_001339"
     CDS             complement(16891..17760)
                     /locus_tag="VQ846_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET3075915.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(17738..18238)
                     /locus_tag="VQ846_001340"
     CDS             complement(17738..18238)
                     /locus_tag="VQ846_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET3075916.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TISEAVSDGANDSLGYIWMDIQEITEENASPLVFKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(18249..18947)
                     /locus_tag="VQ846_001341"
     CDS             complement(18249..18947)
                     /locus_tag="VQ846_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET3075917.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18940..19176)
                     /locus_tag="VQ846_001342"
     CDS             complement(18940..19176)
                     /locus_tag="VQ846_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET3075918.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(19276..20202)
                     /locus_tag="VQ846_001343"
     CDS             complement(19276..20202)
                     /locus_tag="VQ846_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET3075919.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(20277..21092)
                     /locus_tag="VQ846_001344"
     CDS             complement(20277..21092)
                     /locus_tag="VQ846_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075920.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVICIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(21096..21416)
                     /locus_tag="VQ846_001345"
     CDS             complement(21096..21416)
                     /locus_tag="VQ846_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET3075921.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
BASE COUNT         6504 a         4721 c         3807 g         6533 t
ORIGIN      
        1 tcgtcaagaa gaatcaccaa atccctcgta tcatcaacca aaaaattgcg caaaagttaa
       61 ttgaaaagaa ttctatgacc gatattgccc atcagctggc catttcaact tcaactgtca
      121 ttcgaaagct caatgatttt cactttaaac atgatttttc ttgtcttcct gagattatgt
      181 cttgggatga gtatgctttt acaaagggaa agatgagctt cattgcgcaa gattttgaaa
      241 agctcgatat catcactgtt cttgaaggta gaacacaagc tgtcatccga gatcactttc
      301 ttaaatatga tagagccgtc cgatgtcgcg tcaaaatcat tactatggat atgtttagcc
      361 cttactatga cttggctaaa cagcttcgct ttcaaatttc taggctcagg ctgaaacagt
      421 ctcccaggct gtttcactcc cgaatgctaa aatcgttctt gatcgctttc atattgtaca
      481 acatcttagc cgtgctatga gtcgtgtgca tgtccaaatc atgaatcagt ttcatcgaaa
      541 atcccatgaa tacaaggcta tcaagcgcta ctggaaactc attcaacagg atagccgtaa
      601 actgagtgat aagcgatttt atcgtcctac ttttcgtatg cacttaacca ataaagagat
      661 tttaaacaag cttttgagtt attcggaaga cttgaaacac cactatcaac tctatcagct
      721 cttgcttttt catttccaga ataaggaacc agagaaattt ttcggactta ttgaggacaa
      781 tcttaagcag gctcatccta tttttcagac tgtctttaaa accttcctca aagataaaga
      841 aaagattatc aacgcccttc aactacacta ttctaatgcc aaactggaag cgaccaataa
      901 tctcatcaaa cttatcaagc gcaatgcctt tggttttcga aactttgaaa acttcaaaaa
      961 acggattttt atcgctttga acatcaaaaa agaaaggacg aaatttgtcc tttctcaagc
     1021 ttagctgact tcaacccact acagttgaca aagagcccag attaggaact atccgtgagt
     1081 tctctagtct ggagattttt caatagactt cgttattgaa cggttaccaa gttactcaaa
     1141 gtacagattt gaagctgcag ataagactga cgaaatcact ttaatactaa atgaaaatca
     1201 aaaagcaaac taagagcctc aggctgtact tgagtacggt aaggcgacgc tgacgtgatt
     1261 tgaagagatt ttcgaagagt ataaaacact gttttgaggt ggtggataaa tcggctgggc
     1321 aaaaactcaa tctcaggaga aaatgaagta aatctcccca caactataga aaaagatttg
     1381 attctgctcc tcacacaata aaatcttttt cttttattaa aaacgaatcg tttcacgtgt
     1441 tttctcatat gaggtaacaa agcgattggt tacaccaggc tctgccactt ccaatgcttg
     1501 agaaatcttg tcaaatgaag cgtgataatc aaattctttt tcaaaaatat ctaaagcttc
     1561 gttaaatgct tcttgaatgc gttcatcaaa tgagcgatag cggttagaat attgcaagag
     1621 ttgctctgtc aaagttgcat attgtacaat attataagtt tccgtttcta aagcttccat
     1681 atcattcgtt gcaatttcaa gaactcgggt aacagattca atgttaatca ttttttgttc
     1741 taactcaacc attaaatcct cggtattatt gcttgccgta aagaataact tcaagaaagt
     1801 ttgtggaata cctggcagat tgcgtttttc catgtatcgc ttgatagtat ggagacgatt
     1861 gacataaaca ttggcctttt gacgtgcatt aatatcatct ttctcaattt gtgtcaggcg
     1921 ctcactaact gaaatttgct catcttcaat atcttttagt tgagtttgta aatcctcaag
     1981 attttcttca agaactgaat aagcttgggt tggttcttct tgatttgaag ttacctcaac
     2041 aatagctgcc tcaaaactct ctaattctgt ctgaatacga cgaacatggc ttgcagctgt
     2101 ctcaggaagt aaataggtct tgttcaaacg tgcaatatct tctcccaata aagtattatt
     2161 ctctttcata tgttgaaggt aagttggaag agttgcaagt agattttcca ctactttctg
     2221 agcagcaatt tctcgagtaa aaatatcata caaggcattg atttcctctt gtgcctgtcc
     2281 attctcatat tcggcattat ccaattccaa ctgacgaata ttctcttggt ttttcttgaa
     2341 tgcttcataa agcaagtgga aacgcgcttc aatatccgtt tcaacaaaat gataattagc
     2401 atcaattagt ttacgataac cggcttccaa atcctgtaat tgatctggca attctgtaga
     2461 aagcgtagta accaaggctg gaacacgatc cacaatatga cttaaggcca aaatgtgatt
     2521 ttctgtatta tccaaaatca ctgcggcttc cacagggtca cccgatgaat tcaaggttac
     2581 aaattgtgaa aattcagatt ggatattttc taattgtttt tcaatttcat ccaaggcttg
     2641 accatactgt tctgaatttt cagcaactct atgctgaagt tcctcaaata aatccaaagc
     2701 atgaagaaca cgaccactat ttttagattc ttgcttctct aagtctgcca aagcattgcg
     2761 aattgccgca atatcttctt caatcaaagt aatttgactc tcaatttggt caatttgatg
     2821 actggccttg agaaaacgaa atgaatggtt atagccctct gcttcaaaga gattattttc
     2881 aatatcggca aaagagttga gagataaatc gacccatttt tgattccatt cacggaaagc
     2941 cacttgactt tgtccaatca agtgcatatt ttttacagct tctacttcat catttactgg
     3001 aagattgtat agttcttctt ttctttcttc tagtgcctct aatctcccct cgtttcgctt
     3061 acgtagaaag attgccacta cataagccag aactaaaatg actgcaattg caaccattaa
     3121 ataaattagt tgtccattag acatatcaaa ctcctttttt acttgaaaca atcgtaacga
     3181 ttatatcata ttttttagac caagggaact acttctccct attttttaga catcaagtgt
     3241 actatagaca gcattttctt cgataaactc acgacgaggc tctactcgat cccccatcaa
     3301 catatcaaag attttatctg cttctgcagc atcatctaca gaaactctag ccatcaagcg
     3361 atgttcggga tccatggttg tttcccacag ctgatgatcg tccatttcac ctagcccctt
     3421 ataacgctga atagtcggtt tggtacgacc ttcactataa cgggctaaag cttcttggag
     3481 tttgatttct tgatctgcac ccggctggat atattcttta atctcgcttc caaccttgac
     3541 accatagatt ggtggttggg caatataaac ataaccagct tctaggattg gtttcatata
     3601 acgataaatc aaggttaaaa gaagggtacg aatatgggct ccatcgacat cggcatcggt
     3661 catcaaaacg agtttttggt aacgggcttt cgaaacatca aattctgcgc caaatcctgt
     3721 tcccatggct gtgaaaagac tacgaatttc ttcgttagct agaatcttat ccatacttgc
     3781 tttttcaacg ttcaaaatct taccgcgaat tggaaggata gcctgaaact cacggttacg
     3841 accagatttg gctgatccac cagctgagtc tccttcgacg atgaagagtt ctgtttcagc
     3901 agggttatta gaagaacagt ctgctagttt ccctggaagg ttggaaattt ccaaaccaga
     3961 ttttttacgt gtgacttcac gcgcacgctt ggcagccaca cgagccttgg cagccaaaat
     4021 tcctttttct acgatacgtt tggcaatctg tggattttcc atgaggaaat cggagaaggc
     4081 ttcactgaag aggcgattgg taatcttgac cacttcgcta tttcccaatt tggtcttggt
     4141 ttgtccttca aactgtggat ttgggtgttt aactgagata actgcagtta atccttcgcg
     4201 aacatcttcc cctgttaaat tatcttcatt gtctttcagt aacttattct tacgagcata
     4261 atcattgata acacgagtca gtgccgtacg gaaaccttgt tcatgcgttc caccttcatg
     4321 ggtatgaata ttattggcga aactcatgac attttcatgg taacctgttg tgtactgcat
     4381 ggctacctca actgtgatat catccatctc accgtctgta tagattggtg tatcaaagat
     4441 tacatccttg ttctcgttga tatattcaac gtaactagca atcccacctt cataatgata
     4501 atgcttggtt tgttccaaac cttggcgctt atctgtaatt gaaatttgaa gaccgcgatt
     4561 tagaaaggcc aactcttgaa tccgtttatt taatttatca aaatcaaaga ttgttgtttc
     4621 agtgaagatt tttgggtccg gtgtgaagtg aacagttgtt cctgttttat ccgtatctcc
     4681 aactatttca agatctgcga caacatgacc acgacggtat tcttggtaat gaatcttacc
     4741 atttttgtga acatgaacgt ctaattgagt ggaaagggca ttaactactg acgaccccac
     4801 cccgtgaaga ccacctgaaa ccttgtatcc accaccgccg aactttcctc cagcgtgaag
     4861 gactgtaaag acggtctcaa cagcaggacg gcctgttttt tcctgaatat cgactgggat
     4921 accacgccca tcatccacaa cagtaatcga atcatctggc tcaataaaaa cttgaatatg
     4981 gctggcaaat cctgccaagg cctcgtcaat tgagttatca acaatttccc agactagatg
     5041 gtgaagacct tcttttgagg ttgatccaat gtacatccct ggacgcatac gaacagcctc
     5101 taagccctct aaaacttgaa tttgactggc atcataatcc tgtgcctgca gatttttgat
     5161 ttcttctgtc atcttattcc tttttcttat atgtctaata cttgtcttaa attcacgata
     5221 ctggtaaaca agtgggtatc aagtcctgca gcttgacctg cttcgatatc aatcggccga
     5281 tcaccaatga caagaccaga gctaatctga tacttttctc ttaaataaag catggattcg
     5341 ggatttggct ttctcttaaa gcctgagcta gaagtcacca cttctgtaaa ataagctgct
     5401 atagaggttt tttctaaaat ttccaaaacc tgatcatttc gatgagagac caaaaaatga
     5461 cggccacctt gatttgaaat gacttccaat aggtcagaaa ctccttcaaa taaaatcggg
     5521 tgttcaagct ctctggcttc attttccttg tacttttcta aaaaattctc taaattggga
     5581 gcgaatgtct caatcgcaaa aggagtagaa acctttaaag cttgatagac actgtcatgg
     5641 tcttgtgtga taccatacag tgccaatgtt tcaacaaatg cagctgttga agtttcataa
     5701 ttatccagta aagttccacc taaatcccag atgtaatcgt gatatttcat atccttcatt
     5761 atagcatttt tttatgaaaa aagcgatgat ttccctgagt tttccacata aaaaacgaga
     5821 gaataactcc cgttttactg atttttacca aagacatctt gatagagcat tcctgttcgt
     5881 aaactctctg cgtcccctcc tagttgctcc accaactcgt aggcaaaggc aagggctgtt
     5941 gaaggacccc gactggttgt caactgacca tctaccacta ctgtttcctt gacgtagtga
     6001 ccatcaagga tttgctcttg aacgccgtca taacaagtgt atcgcttatt tttcaatatc
     6061 tctgcttgat tgagggcaat tggtgccgca caaatggctg ctagtttctt cccttcttgc
     6121 tcgaagcttt gcaattcttg aatcaaggtc tgattatcac gtaaatgtgc agaaccaggc
     6181 atacctccag gaagaacaat catatcatag tctgataaat ctccatcaaa gacatgatct
     6241 gctcttactt ggattgcatg cgaacccgtt acttgctctt caaaaccaac catatcacat
     6301 gtgatattgg ctcgacgcaa gacatctaca actgtcaagg cttcaatttc ttcaaagccc
     6361 tgagctaaca taactgctac ttttaccatg attttctcct tatttaattt gttttaatac
     6421 aacctttttc cgtttgaatg ggacttttcc gctcttttct tcagctagat tagtctggta
     6481 actcatcgat aaaagcaaac caacaccaat cagattactg ataatagctg atcccccttg
     6541 cgaaataaaa ggcaagggaa tccccgtcaa aggaagtagt ccagtcacag caccgatatt
     6601 ctcaaagatg tggaagagca acatcataat caaacctgtg gaaatataag tgtagaactg
     6661 gttatttgat ttaagagtaa tcttcaacat acggtaaatc aacatgagat agagggcaat
     6721 aaccaggaca gagccaataa agccaaaatc ttctgcaata accgtaaaaa tcatatctga
     6781 ctctcgaact gggataagca gattcgaagc attaaatccc tgaccaaata agccaccact
     6841 cccaatggca atctgtcctt gagcctgctg gtaagtcgtt gtttgggcaa actcaaaggg
     6901 attgagccaa gccaaaatcc gattaatttg gtaggtcggc attccaatct ggtgaagaaa
     6961 agctcgtccg tccttgctaa taaagatagc taagaaacca gcaactcctg ttacagcagt
     7021 cacaaatact gggataataa ttttccaaga aacccctgat aataaaacga ttcctgagaa
     7081 aatggctaca aaaaccaaag ccgtccccaa gtcactttga agtgctaaaa gaactaggac
     7141 tggaatggta aagagaatca tccagaaaat taacaaaaag tccagcggaa ccgtgcgtct
     7201 ccattcctta tgtttctttg taaattggac aatgacacga gccaacatga ggatatagga
     7261 tatcttcata aattctgacg gttggaatag ggtaattcca tttattgata cccagttttt
     7321 ggcacccgtt gatgcaacta agcttggatt ataaaataca atcggcaaga tcataagtcc
     7381 caagcctaaa atatatagaa agggggtcac cttccaaaga aattctgtat taaagagcat
     7441 gaccacaaaa ccaatcacaa gccccaaggc aatccaggcg acctgctgcc ctaaaatggg
     7501 cagaatatta ttgggataat catgactaac ggctatatag atagccacca caccgatgac
     7561 cagtagaaaa aatactggca agagcaaact gtaatcgact cttgagtcga gagaacgttt
     7621 catatatact aaccttatac tttcatacaa tactatttat caaagttcat taaaaaatct
     7681 atcaatagcc tcgtcaactt cggatcgaga gatggtttta acagtcgctt cttctgctag
     7741 agatgctact atttgtttgc cgtatcgttt tccgacgatt ctcctatcca aaataagagt
     7801 taaggaacgt tggtattcac gtctcatact tcttcccaaa gcctgtttta aacgaataat
     7861 ggccattggc aattgataat cataaaaggc atttttccct tcttgattca gttcttgatt
     7921 aatctttttc gtcaagggtt cttgaggatt ttggaaagga agcctcggta caacttgaat
     7981 cacaaaagga tggcttgaaa aatcaactcc ctcccagaaa cttgctgcac caagcaagat
     8041 ttgttgttca cctttttcaa agcgtttctt tagctgatga acatccccat ttttatactg
     8101 ggccaagtgg ctaactgtaa gtaaatccga tactgctaga agcatgtctt tagcggtaaa
     8161 gagaaccaaa atcggttgct ggaaagcttg aatttccact agtaaagcag ctacctctct
     8221 ggcatagact tctaaggagg tttctgttac cagagggaaa tctttgacca taaccacttc
     8281 ttgttcctgt tttccccgag attcaatctt gacaaatttg acttcaggat agcctaaaag
     8341 gtctgccaaa gaaaccctct gactaatctc aagagtagct gatactccca agacttggca
     8401 actctcagga agtaaagagg acagaagagt acggcctgat tttgtagaag aaatctgaat
     8461 tttcttttga ctcgtttcag ttacttcaag ccagtaatca ccttcagctg taaaatagcg
     8521 aaccagctca tcaaagcctt ctacttctaa ttctgaaaaa tactgatgga gattgtccag
     8581 agaatctaag atatttttcc tagatttacc agactgaaat tgttctatca agtagagaca
     8641 ctcaaagcga atactttcta gtatccgttg ttgaaccctg ttttcttctc ctactaaagc
     8701 cttatcaatt aaatcgataa tagattgtat atcgtaggtc tcttgaagca gattttctag
     8761 agctaacaaa atcttttgga cttcatcaat aatcagtaaa cggtcactga caaattcagg
     8821 attatcttca agtctggtta cgagataggc atgattagtc actaaaagct tgcaagtctc
     8881 tgccctttct tgactacgtt tccaaaaatc ttccgtcaca aataagttct gggatgataa
     8941 attcccatca tgacgaaggt ctgctagaaa atgttggtaa cggtagagtt gcccgatttc
     9001 atccaaatct cctgtctctg tctcagtaag ccagaccaag acttgcattt taaagcgtct
     9061 aaataagcga ttttcatcat tttcctgcaa ggaatgataa aaggcatcca acttcagata
     9121 attttgtggt ccctttaagc tatgaatatc tgtatggaac acttccttga ggcgtttacc
     9181 ttcttcttcc atgatttgat tttgaagaat ctttgtcgga acactaagaa caatttgtcg
     9241 ctctttggat tgagataaag cgggtaagag atagccatag gttttcccaa tccctgtcgg
     9301 cgcttgaatc agagagacag gttcatcttt caatagcaag ccaacctctt tagcaaaact
     9361 ttcttgttcc tccctcactt caaggttcaa cagagaaata tttttagaaa agtcttgaga
     9421 tagttttcgt ggctccagag aagctgccat tttcttaaaa tacagacctt gaacttggac
     9481 caagtctgga gaactcagga tagattggtt gcgataagtt tcctcaataa ccaggtagga
     9541 ctcatatagg agagcgtcag ccatttccag caagcgttcc aagagacctt taggaagctg
     9601 ggtcatcttt tcccgtaaaa aaagaagtaa ttctgctgta gcttgggcat ctgaaagggc
     9661 tgtgtgtgcg tgtttaagag gaattcctaa ttctcgacac aaaatcggca agctatattt
     9721 ttccagttca gggaaaaaga cctgggccaa ttcgaccgta tcaacacgag ggtttcttag
     9781 ctcatagcct tcaaaaaata aattttccgc caagagatta gcatcaaact gaacattatg
     9841 ggctacaaaa atcccatcct ccaccaagtc aaatattttt ctggcaactt gcgaaaaatc
     9901 aggtgcttgc gccagacgtt ggtctgtcaa tcctgtcagt tctttgatat gagcatccaa
     9961 gggttcatgt ggattgacat ccgtcgtata gtgatcgacg atttctccat cctcaatcac
    10021 gacaattccc acttggataa ttttagcctt actacctgtg ctagttgcct ctaaatccac
    10081 cacaacatag cgattaccaa tcgttttcat tataaaaaat tataccaaaa aaagggccat
    10141 ttggcaccta caaacatagg ataattttca aactcaagaa cactactttt cttaaaaatc
    10201 ccatcgaata gagcattcta gcctgctgat ttttgtaaaa tagtgaagaa atacgaaaaa
    10261 ttgagagaag aaatgtttgc atcttccttc tctcaacgaa tcatgtattt tatttaacca
    10321 tgtcaggatc gtagtcataa aatcctgtat caaggaaacg gtttttaggg tgaagtttac
    10381 gaacttcttc atccagcaag aaggcttgat catggctaaa gttgccctct tgaatcaagt
    10441 tacgcgcttg gataaagagt tttgcaatct caacaaagtt ctgaccagga gtgtagagcc
    10501 cttctagttt ccagcgagta aagccatgct ccaccaattc tgttaatttg atcatcaaat
    10561 caaggtcatt gttggcaaag atatgggtcc catgattatc ttcaaaaatg gaatagtggc
    10621 tctctggatc acttggctca gccaagaaga ggtcacgttt atgcgtcttt tcatcatcga
    10681 tatgtgtaaa gttatagtag ttttgcaaga gtggacgttt agaatgatgg atgacgctag
    10741 caccgtaaac caaaacttca gcaggaattt ccaaaatctc tggcattttg aaaagttcag
    10801 ctgatggaat ttcacgcgcc aaaacagcct cagatgcgcc agccttttgt ccccagaagt
    10861 taatctgacg actgctagtt accatggttg aagcatcgta gatggtctta aatgaataac
    10921 catcgcggtt aactacgtaa aagacgcctg catccccaat cgtaatatag tctgtcttga
    10981 tttcttccaa gaagtctaag aaaggcttga tacggtccat catatcttgg tgcatgagag
    11041 cattgaccgc aacgatcaat tccttaccag catcatgaac caacttagcg atttcacgta
    11101 attggtcata actaaaggtc gttggcagac gaaggccaaa atctttctca ccgacataga
    11161 tacggtctac gccagcttcg agtagttgtt caacttgttc aatactttca gcagttgctg
    11221 taatgacaat cttttccata agaaaaatta taccacattt ctccaaaatc atccatgctt
    11281 tgaaaatgaa aagggacttt attctttttg taacctttct gtaataattc tctgccgaaa
    11341 aaatgataaa ataggtatgt actgttaagg agagaattat gcccgtaaga aaattacaat
    11401 cctatgaggt agactatcaa gaagaattaa accagcagct tcctcattac caagcttata
    11461 cacctgaagc gcaatctgat gccaatctca aggaaatcct attttttatt aatatcgctg
    11521 ttttttgtat ctgtattgct atctttagtt ttatcttttt agcattaaaa ttatcaactg
    11581 ctcttgcctt tgccgcagca atcggattca gcttacttgt tttaaaagtt caacggtcta
    11641 ttatcaaacg aaaacgtaga agataaatcc aaaatatcgc cccagttgga gcgatatttt
    11701 tattttttct ttttagatgg tgtctgaatg gcaatcttca cttcaaagat tgttcccttg
    11761 ggtttattat ctttgacagt aacagttcct tttagagcat ctacaatttg cttggctagg
    11821 gataatccta aaccaaaacc acctttttgc cgggttctag ccttgtctac tcgataaaaa
    11881 cggtcaaaaa ttttcttttt atcttctgtc gaaataccga ttccattatc agaaacaagt
    11941 aaataaagat tgcgatcggt cgccgagata agaaaatcaa tttcaccatc ctcctcagta
    12001 tacttgacgg cattatcgaa aagaatggtc atcagttgtt tcagaagaag ctgatctgtg
    12061 acaattgttc gatggatacg attttcaaaa cggaagacac gattattttc cgaagcaatc
    12121 atctcatagt ttgtgaaagt tgtattaaaa aagctagttg gaacttctgc aagctctggc
    12181 ttaatcccat catctctccg agctaagttc agcaagcttg tcgttaaaaa acgcatattt
    12241 cggacttctt ccaaactcga tgcaatgctt tcactcacat ccataatggt agcttctggc
    12301 ttacgaaaaa gggtctctaa gcgattttgc aaaactgcga gtggagttcg taactcatga
    12361 ctggcatttt ccacaaaaga ctgttgcttc tgcatactct caagcagggg cctaacactg
    12421 accctagcta gatagagact ggcaagtaaa gacaaaatcc agaaactagc catcacgacc
    12481 acaatcaatt gctcatgctt ttgactagcc tgctccaact gactggtatt aatcaagaca
    12541 gcagcatact tgatattggt tgaaaccgaa ctaatattgg tctccatcaa aatcacacga
    12601 tagatttctt cctgcccata gctattaaaa acctgaatct ggtagatatg tcctagttct
    12661 ttcttttcta acttaatctt atccaagccc aaaaatcgat ttccagaaag aagttgagta
    12721 aagtccttat caaagagaat gacttccgta ttggaactga cattaggttt tatttctact
    12781 ttactagcgt ccgccctagc attttctaaa tctttaatct cttctgttgc cctatttata
    12841 gccagctgaa taactgcttg aggattttca ctcagtccat gaagcttatc gtccaccgaa
    12901 gtataaagac tcgaatgcat gacttgtaaa ataatcagag tcattgtaga aaaaatcagg
    12961 gtgaagacac cgaagttgcg gataaaataa ctaaagtcat ccgcatacca tgttttttta
    13021 agtttactga acatctttta aaagataccc aacactacgc aaggtttgca aattctctgc
    13081 aaaagtggtt ccctttaatt tcttacggac ttttgaaaca tagacttcga caaccgaaat
    13141 cgttgtatca ctatcaaatc cccatagacg gtcaaaaatc tgcgtcttag gcaaaatcac
    13201 attttgattt tgaaggaaat aaactagtaa atcgaactct ttccccagca attcgacagg
    13261 agtatcttca actttaacgg tattggttga taaattaacc acgatattcc cataagtcaa
    13321 ggtgttttca ttaaacttcc ctgaacgttt gagaagggcc tgaatccgca ttttaagttc
    13381 ttctaggtag aaaggtttgg tcagataatc atccgctccc agttcaaatc catgtccctt
    13441 gtcatccaaa ctttccttgg cagtcataat cagaactggt gtcgtaattc ccttttcacg
    13501 caattctttt aagacttgga aaccattttt ttctggcaac atcaaatcca gcaaaatcaa
    13561 gtcatagaca ccactctcag cttcgtagag accttcttct ccatcaaata cctgcataac
    13621 atccgcaaaa tcgtctaaaa agtcaaatac tgaatttgac agacctaggt catcctcaac
    13681 caataagatt tttatcatga gaaactcctc cttattaaaa ctattatacc aaatttgcct
    13741 taaaaaaaac tcaactctct gcattttaca tgagatagct gagttttctt tttattttag
    13801 gcttatttat gcatttccgt attgaagaac aactgcttcg actgcagctt tttcacggct
    13861 aatcaagtca acacgtgctg caatttcctt gattcccata ccgatgttac ggctaagagc
    13921 aaggtcagaa agttgcggct caaagaactc cttgtattcc gccaagcgtt gctgagtctt
    13981 aaatacatga gcaggaagga taacaaagct atcaaagctc atatctcctc caagggctgc
    14041 cttaatccaa gcccagtttt cacgcgccca agaccaagct gttttctgag ttgcttgatg
    14101 agctaggaat tggtaatacc aagcagacaa gtcctgtggt ttgaccacaa atttgtcctt
    14161 ccaagaagta atcaagtttt ggatattatc cgcatctgta ctgtatgcaa gagctgctgc
    14221 caactggcgt ttaaagacag catctgttgc gtgagtataa gtatcaagat aaagtgctaa
    14281 caagtcttta gtctcatgat gtttcatctc attaatcaga acttgtgaac gaatagctgc
    14341 tgggagtcct gcaagattct ccttgtgtgt tgcgaagatt tggctagcga cttgactagc
    14401 ttctgcatca tttgagcgaa tcatcatcga aacagccagc tgacgaacca attcatcctc
    14461 atctgattct ccgtctttag cttcaaaacc aagacggtca tagttatgac gagccaattt
    14521 agcaaccagt cctttgaagg ctgtttcagc atccgttcct tcatcaataa agcgctcaag
    14581 agctgaaatc acttgagaaa cagctgaaac caccagataa gactcttcct tagcaagttt
    14641 atcaagaact ggaagcaagt ctgcataaga aatgtgccct gcctcagcca acaaacgacg
    14701 ttcttgaaca atttgcagtt tgcttgtgtt atcaagtgtc tctagctcag caagaacagc
    14761 ttctaacaag tctccttgat agtcggtaat atagtgggca gtattttcag tgttgagacg
    14821 aagagctcct tcattttcag caagaagagc tgcgtagcca gggatttcga tactttcagt
    14881 ttcgagtgta tcaggcaagc ctttccagtt gctattgagg ggcaccaccc agagacggtt
    14941 cttgtcttcg ttctcaccga tgaagaattg tttttgtgaa atcttcaaga catcattttc
    15001 aactttgaca gtaagaactg ggtaaccagg ctgttccaac caagaatcca tgaaggctgc
    15061 gacatcacgt cctgacgctt gaccaagggc atcccaaagg tcactaccaa tggtgttgct
    15121 gtattggtgt ttttcaaagt aggcgtgcaa acctttagca aaatcagcat ctcctagcca
    15181 acggcgaagc atgtgcatga gacggcttcc tttggcatag acgatagcgc cgtcaaagag
    15241 tgtattgatt tcatctggat gtttaacttc gacgtggaca gactgaacgc catcagtagc
    15301 gtcacgttca agagcaagag gtactccacc tgtttggaaa tcttcaaaga tattccagct
    15361 tggttcgatg gtatccacac agacgtattc catcatatta gcgaaacttt cattgagcca
    15421 aaggtcatcc caccatttca tagtcacgag gttcccaaac cattggtgag ccaattcatg
    15481 agccacaaca agggcaactt gttgacggct agcaaatgta gagttttcat cgacaaccaa
    15541 gtaaacttca cggtaggtca caagacccca gttttccata gcaccagctg agaagtcagg
    15601 aagggcgatg tggagagatt gaggaattgg gtacttaact ccatagtaat cttcgtaaaa
    15661 ctcgatagag cgaacagcga tatccagtga gaaatcaaga tttgaaagtg gatgtgcttt
    15721 ggttgagtag acacctacca gggtaccatt tttagtttta gcggtcaccc cttgcaaatc
    15781 accagcaaca aaggccaaca agtaagaaga catgcgaggt gttgtctcaa acttccagat
    15841 acctgtttcc ttacggtttt caacatcgat ttctggcatg tttgacaagg ccaattcacc
    15901 ttctgcttgg tcaaagcgaa gagagaggtc aaaagttgct ttggcttcag gctcatccac
    15961 acatgggaaa gcttcgcgcg caaaatggct ctcgaactga gtagacaaga cctccttctt
    16021 gactccatca actgtataat aagaagggta aatccctgtc atgttgtctg taattttacc
    16081 agaaaaggca agaaccaatt caacttgacc agcctcagcc aattcgatat gaagggcttc
    16141 attgtcatgg tcaactgtaa atggacgagc ttgacctgca acttctacag aggtgatttc
    16201 caagtctttt tggtggaggg agatgcggtc actctgtgct tgaccagtga tggtcacttt
    16261 cccagaaaaa gtcttggtct cacgactcaa atctaaaaat aaatcataat gttcaggaac
    16321 aaattgctta ataaaatgtt caactgcttg catagttttc tcctattcta agtttaagag
    16381 ctgctgctct tcttcaaaca aacttattat atcatatttt gcttgagaaa aaaggattgg
    16441 acagcgattt ccaaaacttt cttccttcta atagtctaca gtgggtagac cttgcgaaat
    16501 tcttctaaaa caaccttgct ttcaggtgta aaagtcagcc ctacttcacc caaacagtct
    16561 gtgaaaaagt cctcatgaac ctggcgccaa taggcaagtg atttgtctcc ctcaccttcc
    16621 ttaaaggcat gttgcgcaga aacttgattg aagagttcaa cagaaacctt tgtaatttcg
    16681 acaatgcaga cagcctgatt ttgactatct aaaatgacat cgaaggtccc tacttgggga
    16741 agtggttcgt cttctagtac atagaggtca taggctgatg ctgttgctgt cttttctcct
    16801 ttaaacacca aatccgctaa aaggtctggt tcaactccaa aagcccaagc atcgatttca
    16861 tctccgatca aaggattgat ttgcttgtat ttattccaca tttcttgcgg tgtcatgggt
    16921 actcctttgt aattttttac tttcttcttt tatgtgttta agatgatctg gatggtcaat
    16981 ctctaaatca aaaatctctg gaatagaact gtagtggata atgcacttga tacccaactg
    17041 attcattttt tgtatgaaag aagtattcag atagcctgct acagcaaaat taatcttgtt
    17101 ctttcttgct ttatcctgca tatctcttag catatctaac attattggac tttccatatc
    17161 atgccattga ctgtttctca tagtcgcaaa aacaaaggaa gtcaaatcat tcattccaac
    17221 tacaatcttt gaaatgcccg tttccagtat actagataag tcaaaatacg ctgacggtaa
    17281 ttcaatcatc gttccgactt tcccagtaaa gccatactga cgcaatactg taatagcttg
    17341 ttttaattgg tcggcatcat tgacaaaagg aaagataaca gatagattgg ggttggtttg
    17401 ataaacttct gtaacgacat gtgcttcagc ctgaaattca tccaaacacg ccagtaaacg
    17461 cctagttcct ctatagccaa acaagggatg cccttcgtca aaaaactctt tagtccccac
    17521 taaacaattg gcttctgtat tcgttaattc agtaaaacga taccaaactt cttcatctga
    17581 gtacagggag cagatagtct ctagataatc ttttacaaat tgctgacaac ttgctaatat
    17641 gatgttttga ttaagctctc tcaacaaata ttccccacga atcatgccga cgtggtgaaa
    17701 tagttgagga taaatttttt caagaatttt ttcgccacta agggcaagtt gatttctcat
    17761 cattcacctt ccaattcatg taagaagtct tgtccagttc tggaaatcct aataattcag
    17821 acttaacctt aaagactaat ggcgatgcat tttcttctgt aatctcttga atatccatcc
    17881 aaatatatcc aagtgaatca ttcgcaccat cagacacagc ttccgaaatc gtaacttgag
    17941 gtgcactctc attcatttca acatcataca aggccatgac atggtgaacc ataaaatttt
    18001 ttaactcttc tctgacgaaa acatcgtaga ttcgaggatt agagtagctt ctaacagtaa
    18061 atcccgtctc ttccataact tctctagtca gcgtttccgt cagtccttca ccaagttgct
    18121 gactgcctcc aggtagatca taccgatgtt gataagggcc tctcgttttt tcaatgcaga
    18181 gtaacttccc attttctaag caaacagcgt agactccaaa gtgttttttg atttccatcc
    18241 aactcctcct acttcaaaga ccagccacca tctattgtca agatttgtcc ttgcatggcg
    18301 ctcgcttttc cacttgctaa aaaaagacta agctctgcta tttcctctgg ctcaatccag
    18361 cgcttgattg gggtttcact agccacccag tcagccaaac cacctggttc aaaatccgca
    18421 gcggtcatag ctgtcttgac tgctcctgga gcgataccaa agacctgaat cccagcttca
    18481 gcatagtcta gagccaactg cttggtgaag ccagccaagg catgcttgga tgaagtatag
    18541 gcgtgaccac ctccacctgc taggctagaa gcaatggaac acatattgat gatgattccc
    18601 tttttatttt ccagcatttg tgtcaaataa taccgagtca actctactgg aataatgtag
    18661 ttgatttcaa aaatctcttg aatgtcctgc gccgtttgtt ccaacagtgg tttgtaatca
    18721 tccaaaactc cggcagtatt acacaaaaca tccacctgag ggcaccagtc aaaaataggt
    18781 tccaagtcca aggtcaaatc tctctgtaaa aagtgaaaat caccctctaa gagtggcttt
    18841 tcaccttggt caactccata aacttgatag cccttctcta aaaagaggcg agcttgagcc
    18901 aatccgatcc ctgaactcac tcctgtaatg agtacacgtt tagtcatgca cttctaccca
    18961 atccgttgcc aaaacatcac aagctgtcgg gctccacatg gaaaaacctt ctccttctcc
    19021 agacacgttg attaagaaat aaggtgtcac ttcaagtgca agcccatttt gctcgatggt
    19081 atcaaagagt tggacatagt tttcagcacc tccccaacca gttcgtacat attttttctt
    19141 agcctttaag ccaggtagaa tttcttcaaa tgtcatgttt ttctccttta atgtcaataa
    19201 ttctcccctt cattataaca aaaaaaccgc tttaaaacgg ctttttgact gtgatttatt
    19261 tacatcttct tctacttacg gcaaattatt ccctgcagca agataaattt cataccattc
    19321 ttttcttgtt aagctaaagt ttgccgcttg gctaacttct ctcaagtgct taggatttgt
    19381 ggtacctaca actgcctgca tttttgctgg ataacgcaat atccaagaaa tggcaatagt
    19441 tgaagaggtt actccatatt taatagctaa acgatcaagt acttgattta aagcttgaaa
    19501 tttctcattt ccaacaaaat tccctttaaa atacccgaat tgtaagacag accatgcttg
    19561 aatgaccaca tcgtgtaatt ggcaatattc aaaaatgctg ccatctcgca tagctgcttg
    19621 actatcttcc atattaacat gaaaagctga ttcaaatcct ggagtaaaag ccgcactcaa
    19681 ttgtagctga ttaacagcta acggctgctt gacatctttt ttaagcaact ccatcatcat
    19741 aggattttga ttagaaactc caaaatctcg aactttacct tgtttataaa ggagatcaaa
    19801 ggcttctgct acttggtcag attccatcaa agcatctggt cgatgaagga gcaagctatc
    19861 tagatgatca atcttcaatc tttgcaaaat accgtctact gattttataa tatagtcctt
    19921 agaaaaatca aaataggtaa attcttcaat gcgaatgcca catttggact gaatccacat
    19981 cttttctctt aaatctggac gattttttag gacaagacct aacagttctt cacaacgacc
    20041 acgaccataa atatcagcca agtcgaaggc attgattcca acagaaagtg ctgtttctac
    20101 aagctcttca acttctttta cagacttatc ttttattctc atcattccga gaacaatttc
    20161 tgataattct ttgtcatctt gaccaagagt tatgtatctc atcaaatttt tctcctttaa
    20221 tttctaacat tcttcccttc attataacaa aaaaccgctt tgcaacggct ttttgactat
    20281 acttcactcc attttatctt cttaaaccca cggaacaaga caaagattcc aataaagagg
    20341 acagctaaag gaataacttt tgtaaggaaa acatttgaaa ttcccatcca ctcataataa
    20401 cggagcagag aacccaccac aagatgggca ataatcatac tgacaaatgg acgaaagacc
    20461 gcttctttcc aattccaaat acagataact agcgaaatcg taaagacaga caaactatcc
    20521 cagggagccg gaatataaaa ggctccttct tgtatgaagc ttgccattcc tacatatcct
    20581 aaaacaacta aaagaactat agtcccaaca acaatgtaag tgccaatttt cattttagga
    20641 gaatcttgga ctaaacttct tcgtaaaatt gtggccacaa gtccaaatcc aatcagaaaa
    20701 ataagaagtt gccctaaaaa tgtgagcaaa ttgactgtta agagaggacc tttagaaaaa
    20761 tcacttagta gttgataata acgtaatacc gccaggacaa gaattggcgt caaaagggac
    20821 tctttgatag aactgcgagg tgctcccttg agaatctctt tcattatttt tttaggattc
    20881 ttacctagat aatcctctgc actcatgcca tctcgttctg cttctgagaa atctagcatc
    20941 atcaaataga tctgctctct gagatagtct tcatcataga gaaatccagc aagattaaaa
    21001 ctttcccaca actcctcaaa atacttttga ttctcctcag aaaactcatg tagcaaagcg
    21061 cttgtttctt cgtaatactt cattttcttc atggtttaac ccccattctt aatcccttct
    21121 actttttgac tcaaatcgtc ccattgttgc caaaagactg agacacgctc ttctccttct
    21181 ttcattaatg aaaaatactt ccgatctgga ccatctggcg acgggcgcat gtcgcctctt
    21241 atccattgat ttttttctaa cttttgcaac aaaggataaa tagttcctgg aacgatagta
    21301 tcaaatccag cctctcgcaa agtctgaacc aactcataac cataccgctc tttttgacca
    21361 atcatatcca agacacaacc ttcaagaaca ccttttaata gctgagtttc tttcatcact
    21421 tctcccttct aatctatttt gtaataccta ctagtaactt cacctatagt atatcacttc
    21481 tacactagtt tgtaaagcat aatagttaat actcttcgaa aatctcttca aaccacgtca
    21541 gcgtcgccct accgtatgta tggtt
//
LOCUS       DAVBNO010000033        20672 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVBNO010000033 DAVBNO010000000
VERSION     DAVBNO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20672
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.033"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            177..2159
                     /locus_tag="VQ846_001346"
     CDS             177..2159
                     /locus_tag="VQ846_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET3075922.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVQFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            2459..7762
                     /locus_tag="VQ846_001347"
     CDS             2459..7762
                     /locus_tag="VQ846_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET3075923.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWKTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKNVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGTTTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKAIITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDEAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGNMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIETLKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALTKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASEQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(7929..10088)
                     /locus_tag="VQ846_001348"
     CDS             complement(7929..10088)
                     /locus_tag="VQ846_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET3075924.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQSAQP"
     gene            complement(10085..10681)
                     /gene="recU"
                     /locus_tag="VQ846_001349"
     CDS             complement(10085..10681)
                     /gene="recU"
                     /locus_tag="VQ846_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET3075925.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKRQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10747..11274
                     /locus_tag="VQ846_001350"
     CDS             10747..11274
                     /locus_tag="VQ846_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET3075926.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            11344..11673
                     /gene="gpsB"
                     /locus_tag="VQ846_001351"
     CDS             11344..11673
                     /gene="gpsB"
                     /locus_tag="VQ846_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET3075927.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11694..12074
                     /gene="rnpB"
                     /locus_tag="VQ846_001352"
     ncRNA           11694..12074
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VQ846_001352"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12159..13316
                     /locus_tag="VQ846_001353"
     CDS             12159..13316
                     /locus_tag="VQ846_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET3075928.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13329..14723
                     /gene="mapZ"
                     /locus_tag="VQ846_001354"
     CDS             13329..14723
                     /gene="mapZ"
                     /locus_tag="VQ846_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET3075929.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            14799..16223
                     /gene="gndA"
                     /locus_tag="VQ846_001355"
     CDS             14799..16223
                     /gene="gndA"
                     /locus_tag="VQ846_001355"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET3075930.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16235..16924
                     /locus_tag="VQ846_001356"
     CDS             16235..16924
                     /locus_tag="VQ846_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3075931.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            17022..18041
                     /gene="cbpC"
                     /locus_tag="VQ846_001357"
     CDS             17022..18041
                     /gene="cbpC"
                     /locus_tag="VQ846_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET3075932.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPISEVAKGWTQDFHVTIDIDRSKPAPWYYLDPETGIM
                     RTGWQYLGNNWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSS
                     GAMATGWYQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYN
                     GEWVR"
     gene            complement(18159..19391)
                     /locus_tag="VQ846_001358"
     CDS             complement(18159..19391)
                     /locus_tag="VQ846_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET3075933.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLKLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAYIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(19551..20021)
                     /locus_tag="VQ846_001359"
     CDS             complement(19551..20021)
                     /locus_tag="VQ846_001359"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075934.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCGIAY
                     ALFAYSKVLEPSMFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKSNR"
     gene            complement(20188..20598)
                     /locus_tag="VQ846_001360"
     CDS             complement(20188..20598)
                     /locus_tag="VQ846_001360"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075935.1"
                     /translation="MQLLELSHTQGQLTDEQLFWLQCLLNLSQTNNLVKIKKIEEHKY
                     DYKTECNSVLRFLLDKTVNSEGRITSSTEFGNFVSNLSFQHGIAGLLFPLNKLYHPEL
                     DSKILSIIKKAVTIRTSHTYEYQYSLLFGGAGYL"
BASE COUNT         6424 a         3744 c         4636 g         5868 t
ORIGIN      
        1 tccgtgagtt ctctagtctg gagatttttc aatagacttc gttattgggc ggttacgata
       61 ttcatatttt ttgcaaagat gttgtttgaa aaataatttt caaaaattct gaaaattctg
      121 ttgacatctt tctgaaaaga gtctataatg gagagaaagt tttaaaggag aaaatgatga
      181 aaagttcaaa actatttgcc cttgcgggcg tgacattatt ggcggcgact actttagctg
      241 catgctctgg atcaggttca agtactaaag gtgagaagac attctcatac atttatgaga
      301 cagaccctga taacctcaac tatttgacaa ctgctaaggc tgcgacagca aatattacca
      361 gtaacgtggt tgatggtttg ctagaaaatg atcgctacgg gaactttgtg ccgtctatgg
      421 ctgaggattg gtctgtatcc aaggatggat tgacttacac ttatactatc cgtaaggatg
      481 caaaatggta tacttctgaa ggtgaagaat acgcggcagt caaagctcaa gactttgtaa
      541 caggactaaa atatgctgct gataaaaaat cagatgctct ttaccttgtt caagaatcaa
      601 tcaaagggtt ggatgcctat gtaaaagggg aaatcaaaga tttctcacaa gtaggaatta
      661 aggctctgga tgaacagaca gttcagtaca ctttgaacaa accagaaagc ttctggaatt
      721 ctaagacaac catgggtgtg cttgcgccag ttaatgaaga gtttttgaat tcaaaaggag
      781 atgattttgc caaagctacg gatccaagta gtctcttgta taacggtcct tatttgttga
      841 aatccattgt gaccaaatcc tctgttgaat ttgcgaaaaa tccgaactac tgggataagg
      901 acaatgtgca tgttgacaaa gttaaattgt cattctggga tggtcaagat accagcaaac
      961 ctgcagaaaa ctttaaagat ggtagcctta cagcagctcg tctctatcca acaagtgcaa
     1021 gtttcgcaga gcttgagaag agtatgaagg acaatattgt ctatactcaa caagactcta
     1081 ttacgtatct agttggtaca aatattgacc gtcagtccta taaatacaca tctaagacca
     1141 gcgacgaaca aaaggcatcg actaaaaagg ctctcttaaa caaggatttc cgtcaggcta
     1201 ttgcctttgg atttgaccgt acagcctatg cctctcagtt gaatggacaa actggagcaa
     1261 gtaaaatctt gcgtaatctc tttgtgccac caacatttgt tcaagcagat ggtaaaaact
     1321 ttggcgatat ggtcaaagag aaattggtca cttatgggga tgaatggaag gatgttaatc
     1381 ttgcagattc tcaggatggt ctttacaatc cagaaaaagc caaggctgaa tttgctaaag
     1441 ctaaatcagc cttacaagca gaaggagtcc aattcccaat tcatttggat atgccagttg
     1501 accagacagc aactacaaaa gttcagcgcg tccaatctat gaaacaatcc ttggaagcaa
     1561 ctttaggagc tgataatgtc attattgata tccaacaact acaaaaagac gaagtaaaca
     1621 atattacata ttttgctgaa aatgctgctg gcgaagattg ggatttatca gataatgtcg
     1681 gttggggtcc agactttgcc gatccatcaa cctaccttga tatcatcaaa ccatctgtag
     1741 gagaaagtac taaaacatat ttagggtttg actcagggga agataatgta gctgctaaaa
     1801 aagtaggtct atatgactac gaaaaattgg ttactgaggc tggtgatgag gctacagatg
     1861 ttgctaaacg ctatgataaa tacgctgcag cccaagcttg gttgacagat agtgctttga
     1921 ttattccaac tacatctcgt acagggcgtc caatcttgtc taagatggta ccatttacaa
     1981 taccatttgc attgtcagga aataaaggta caagtgaacc agtcttgtat aaatacttgg
     2041 aacttcaaga caaggcagtc actgtagatg aataccaaaa agctcaggaa aaatggatga
     2101 aagaaaaaga agagtctaat aaaaaggctc aagaagatct cgcaaaacat gtgaaataac
     2161 tgttgcaaaa tataagaaag gatttagtat ttctcttgaa tgctgaatcc ttttttacat
     2221 ttgtaaagaa agcttctaaa tgtactgacc cccaaaagtt ggagcctctt tttgtcagaa
     2281 tagagaaaat ttttgttaat tttacttgtt tcctattgct ttctcagtta ttatttgtta
     2341 tattaaaagt ataattattt tttatttatc agggttaagc attgcacttt cagaggaagg
     2401 agtatttttt aaaaaggaaa tgtaaacgct tgctaaaaat gaaaggattt agaagtttat
     2461 gaataaagga ttatttgaaa aacgttgtaa atatagtatt cggaaatttt cattaggtgt
     2521 tgcttctgtt atgattggag ctgcattctt tgggacaagt ccggttcttg cagatagcgt
     2581 gcagtctggt tccacggcga acttaccagc tgatttagct actgctcttg caacagcaaa
     2641 agagaatgat ggacgtgatt ttgaagcgcc taaggtggga gaagaccaag gttctccaga
     2701 agttacagat ggacctaaga cagaagaaga actattagca cttgaaaaag aaaaaccggc
     2761 tgaagaaaaa ccaaaagagg ataaacctgc agctgctaaa cctgaaacac ctaagacggt
     2821 aacccctgaa tggaaaacgg tagagaaaaa agaacaaaag ggaacagtca ctatccgaga
     2881 agaaaaaggt gtccgctaca accaactatc ctcaactgct caaaatgata acgcaggtaa
     2941 accagccctg tttgaaaaga agggcttgac cgttgatgcc aatggaaatg caactgttga
     3001 tttaaccttc aaagatgatt ctgaaaaggg caaatcacgc tttggtgtct tcttgaaatt
     3061 taaagatacc aataataatg tttttgtcgg ttatgacaag gatggctggt tctgggagta
     3121 taaatctcca acaactagca cttggtatag aggtagtcgt gttgctgctc ctgaaacagg
     3181 atcaacaaac cgtctctcta tcactctcaa gtcagacggt cagctaaatg ccagcaataa
     3241 cgatgtcaat ctctttgaca cagtgactct accagctgcg gtcaatgacc atcttaaaaa
     3301 tgagaagaag atccttctca aggcgggctc ttatgacgat gagcgaacag ttgttagcgt
     3361 taaaacggat aaccaagagg gggtaaaaac agaggatacc cctgctgaaa aagaaacagg
     3421 tcctgaagtt gatgatagca aggtgactta tgacacgatt cagtctaagg tcctcaaagc
     3481 agtgattgac caagccttcc ctcgtgtcaa ggaatacacc ttgaatggac atactttgcc
     3541 aggacaggtg caacagttca accaagtctt tatcaataac caccgaatca cccctgaagt
     3601 cacttataag aaaatcaatg agacaacagc agagtacttg atgaagcttc gcgatgatgc
     3661 tcacttaatc aatgcggaaa tgacagtacg cttgcaagtt gtggacaatc aattgcactt
     3721 tgatgtgacc aagattgtca accacaatca agtcactcca ggtcaaaaga ttgatgacga
     3781 aagaaaacta ctttcttcta ttagtttcct cggcaatgct ttagtctctg tttctagtga
     3841 tcaaactggt gctaagtttg atggggcaac catgtcaaac aatacgcatg tcagcggaga
     3901 tgatcatatc gatgtaacca atccaatgaa ggatttggct aagggttaca tgtatggatt
     3961 tgtttctaca gataagcttg ctgctggtgt ttggagtaac tctcaaaaca gctatggtgg
     4021 tggttcgaat gactggactc gtttgacagc ttataaagaa acagtcggaa atgccaacta
     4081 tgtaggaatc cacagctctg aatggcaatg ggaaaaagct tataagggca ttgttttccc
     4141 agaatacacg aaggaacttc caagtgctaa ggttgttatc actgaagatg ccaatgcaga
     4201 caagaacgtt gattggcaag atggtgccat tgcttatcgt agcattatga acaatcctca
     4261 aggttgggaa aaagttaagg atatcacagc ttaccgtatc gcgatgaact ttggttctca
     4321 agcacaaaac ccattcctta tgaccttgga tggtatcaag aaaatcaatc tccatacaga
     4381 tggtcttggg caaggtgttc tccttaaagg atatggtagc gaaggccatg actctggtca
     4441 cttgaactat gctgatattg gtaagcgtat cggtggtgtc gaagacttca agaccctaat
     4501 tgagaaggct aagaaatatg gagctcatct aggtatccac gttaacgctt cagaaactta
     4561 tcctgagtct aaatacttca atgaaaaaat tctccgtaag aatccagatg gaagctatag
     4621 ctatggttgg aactggctag atcaaggtat caacattgat gctgcctatg acctagctca
     4681 tggtcgtttg gcacgttggg aagatttgaa gaaaaaactt ggtgacggtc tcgactttat
     4741 ctatgtggac gtttggggta atggtcaatc aggtgataac ggtgcctggg ctacccacgt
     4801 tcttgctaaa gaaattaaca aacaaggctg gcgctttgcg atcgagtggg gccatggtgg
     4861 tgagtacgac tctaccttcc atcactgggc agctgacttg acctacggtg gctacaccaa
     4921 taaaggtatc aacagtgcca tcacccgctt tatccgtaac caccaaaaag atgcttgggt
     4981 aggggactac agaagttatg gtggtgcagc caactatcca ctgctaggtg gctacagcat
     5041 gaaagacttt gaaggctggc aaggaagaag tgactacaat ggctatgtga ctaacctctt
     5101 tgctcatgac gtcatgacta agtacttcca acacttcact gtaagtaaat gggaaaatgg
     5161 tacaccggtg actatgaccg ataacggtag cacctataaa tggactccag aaatgcgagt
     5221 ggagttggta gatgctgaca ataataaagt agttgtaact cgtaagtcaa atgatgtcaa
     5281 tagcccacaa tatcgcgaac gtacagtaac gctcaacgga cgtgtcatcc aagatggttc
     5341 agcttacttg actccttgga actgggatgc aaatggtaag aaactttcta ctgagaagga
     5401 aaagatgtac tacttcaata cgcaggccgg tacaacaact tggacccttc caagcgattg
     5461 ggcaaagagc aaggtttacc tttacaagct aactgaccaa ggtaagacag aagagcaaga
     5521 actaactgta aaagatggta aaattaccct agatcttcta gcaaatcaac catacgttct
     5581 ctatcgttcg aaacaaacca atcctgaaat gtcatggagt gaaggcatgc acatctatga
     5641 ccaaggattt aatagcggta ccttgaaaca ttggaccatt tcaggcgatg cttctaaggc
     5701 agaaattgtc aagtctcaag gggcaaacga tatgcttcgt attcaaggaa acaaagaaaa
     5761 agttagtctc actcagaaat taactggctt gaaaccaaat accaagtatg ccgtttatgt
     5821 cggtgtcgat aaccgtagta atgccaaggc gatcatcact gtaaacactg gtgaaaaaga
     5881 agtgactact tataccaata agtctctcgc tctcaactat gttaaggcct acgcccacaa
     5941 tacacgtcgt gacaatgcta cagttgacga tacaagttac ttccaaaaca tgtacgcctt
     6001 ctttacaact ggatcggacg tctcaaatgt tactctgaca ttgagtcgtg aagctggtga
     6061 tgaagcaact tactttgatg aaattcgtac ctttgaaaac aattcaagca tgtacggaga
     6121 caagcatgat acaggtcaag gaaccttcaa gcaagacttt gaaaatgttg ctcagggtat
     6181 cttcccattt gtagtgggtg gtgtcgaagg tgtcgaagac aaccgcactc acttgtctga
     6241 aaaacacgat ccatatacac aacgtggttg gaacggtaag aaagttgatg atgttatcga
     6301 aggaaattgg tcactcaaga caaatggact agtgagccgt cgtaacttgg tttaccaaac
     6361 tattccgcaa aacttccgtt ttgaagcagg taagacctac cgtgtaacct ttgaatacga
     6421 agcaggttca gacaatacct atgcttttgt agtcggtaag ggagaattcc agtcaggtcg
     6481 tcgtggtact caagcaagca acttggaaat gcatgaattg ccaaatactt ggacagattc
     6541 taagaaagcc aagaaggcaa ccttcctcgt gacaggtgca gaaacagggg atacttgggt
     6601 aggtatctac tcaactggaa atgcaagtaa tactcgtggt gattctggtg gaaatgccaa
     6661 cttccgtggt tataacgact tcatgatgga taatcttcaa atcgaagaaa ttaccctaac
     6721 aggtaatatg ttgacagaaa atgctctgaa gaactacttg ccaacggttg ccatgactaa
     6781 ctacaccaaa gagtctatgg atgctttgaa agaggcggtc tttaacctta gtcaggccga
     6841 tgatgatatc agtgtggaag aagcgcgtgc agagattgcc aagattgaaa ccttgaagaa
     6901 tgctttggtt cagaagaaaa cggctttggt agcagatgac tttgcaagtc ttacagctcc
     6961 tgctcaggct caagaaggtc ttgcaaatgc ctttgatgga aacttatcta gtctatggca
     7021 tacatcttgg aatggtggag atgtaggcaa gcctgcaacc atggtcttga aagaaccaac
     7081 tgaaatcact ggacttcgtt atgttccacg tggatcaggt tcaaatggta acttgcgtga
     7141 tgtgaaactt gttgtgacag atgagtctgg caaggagcat acctttactg caactgattg
     7201 gccagataac aataagccaa aagacattga ttttggtaag acaattaagg ctaagaaaat
     7261 tgtccttaca ggtactaaga cttacggaga tggtggcgat aaataccaat ctgcagcgga
     7321 actcatcttt actcgtccac aggtagcaga aacacctctt gacttgtcag gctatgaagc
     7381 agctttgact aaggctcaga aattaacaga caaagacaat caagaggaag tagctagcgt
     7441 tcaggcaagc atgaaatatg cgacggataa ccatctcttg acggaaagaa tggtggaata
     7501 ctttgcagat tatctcaacc aattaaaaga ttctgctacg aaaccagatg ctccaactgt
     7561 agagaaacct gagtttaaac ttagctcttt agcttccgag caaggtaaga cgccagatta
     7621 taagcaagaa atagctagac cagaaacacc tgaacaaatc ttgccagcaa caggtgagag
     7681 tcaatctgac acagccctct tcctagcagg tgttagccta gccctatctg ctctctttgt
     7741 agtaaaaacg aagaaagact agtatttagt aaaacctctt aacaagatta cggaagcagt
     7801 ctctatcttt tccaatgagg tttatagtac agaaaaagcc tgagaagatg tcttctcagg
     7861 cttttgttaa gcacataaat acaatagtgc tatgaaaaaa tcacccagaa aaatctgggt
     7921 gataaatgtt atggttgtgc tgattgagga ttctgatttt gttgatcagg ggttgtattt
     7981 gattgttgcg tattattgtt aggattggta gtcgtactat tatttgtgct tggagtggtt
     8041 gagctagact gtgaagttga actatctgat gatgagcttg aactttcagt tgatgggggt
     8101 tgttgtggag caggtgagct ccacgtagaa cgagcaccat ttttaaatac gaattctcca
     8161 tttctgtaga gcccctctgg tatattccaa tcctctggat tgcttccttc agacaggtag
     8221 gtcatcatag agcggtaaac tttggcagcg accgtaaggc cattgcctac aagtggtgtc
     8281 agacggttag aatagcctgt ccatacagcc attgaatatt tacgcgtata gccagcaaat
     8341 agttcatcag gtgctacaaa ttgagaggtc ttgatgtggt tttcaatttc ctcgtctgta
     8401 tagttagagg ttcctgtttt accagcctga gggagccaag caagataggc atttcgtcca
     8461 gttccataac tcaagactgt tttcatcatg tcggtcatca tataggctgt cgtttccttc
     8521 atggcacgag ttccgacatt agagaactct ttttcactcc catcactaaa gacgacttta
     8581 tggatataca ttggtttata gtaagttcca ccatttgcaa aggcagcgta agcagcagcc
     8641 atcttttcac tacttgctcc atattttttg tctgattcgg ttgtgttact tgaaatggca
     8701 tttgagtagt gaatacttgg gtagtcgatt ccgagaccat ttaggaaagt cttggcgcgg
     8761 ttgagtccga ccttgtttag agtttccacg gctgggacgt ttcgcgattg ttgcagggcg
     8821 tattgcaagg tgatgttgcc aaagtagccc ctatcccagt tataaacagg agtatttgtc
     8881 ccagggtagt tatagggctc atcgtgaacg atagtagcag ttgaatcgta gacaccgtac
     8941 tccaaggcag gagcatagtc tgtgatcggt ttcatagttg atccccagtc gcggtttgtt
     9001 tctactgctt ggttaattcc gaaggaaaca ttacttgact gatggcgtgc tcctagctgg
     9061 gcaatgactt taccgttaga aacatcaaca atggtagaag cgacttgcaa ttcatcgtct
     9121 ggataggcaa cgtattcgtc tgtattgtaa atatcccaca gatgtttttg agcttcttgg
     9181 tctacatttg tgtagacatc catcccagtt gtgagcaggt tatagcctgt ttcttcttca
     9241 acttgattga tgacttcctt gaggtaatta tccatgtaag cagggtaatt acttgctgat
     9301 ttgagacttt gtagtccatc agtaattggt gtattgactg ctttctcata ctgttcagca
     9361 gagatgtagc cttgattttt catttcagat aagaccaagt ttcggcggtc ttgggctgct
     9421 tctggatgtg aataggggtc atattggttt ggtgcctgag gcattccagc cagcaaggct
     9481 aactgaggta aacttaaatt attgaggtct ttaccatagt agttttgagc tgctgtctgc
     9541 attccatagt tcccattaga catgtagacc ttatttatat agtaggtcaa gatttcttgc
     9601 ttggttgctt tttgttctaa ctgaatcgct aaccaagctt cctgagcctt acgagaaata
     9661 gtctggtcgg aagtcgaagt tgaaaagtaa gtcaacttaa tcaactgttg agtgagagtt
     9721 gatccacctt ggagggaatt gctttgcaga ttgcgcaaga aagctcccag gatacggatg
     9781 gtatcaatcc ccctgtggtc gaagaagcga tggtcttcga tagaaacgat tgccttaacc
     9841 aaatctgtgg gaatatcatt agcttgggca ttgacgcggc gttcagaacc caagtcagca
     9901 atgagttgat ttttattgtc gtagatttta ctagaagttg ttgcaactag tttactctcg
     9961 gataggctag gagccttgct aacgtagtag aaaaaaactc ctccgcctaa gacaatggct
    10021 gcgataacca agcttaagaa gctaatgctc agatacttga ttaggcgcag aatcgttggt
    10081 ttgttcatct tgttttacca cctaataaat gttctttgat aacattgaga taaggaattt
    10141 gagggaaggc accagccttg atttcatatc catattctcg aatatattca agtggcattg
    10201 atttttgtct cttatcttga tgatagaagc gaatcaaatc gaatgccggc aataagtagg
    10261 tttcttgctg agaagaaaag tgaagaagga caaagcagat tccttgttgg gcaaggactt
    10321 gttccatatg ctgaatctga tgtggatgaa aatttttcat cggaatcgca cgtttttgtt
    10381 ttgtttcctt gacttcaaag tcgatgtaat atccattata aacgccagaa tagtccgtcg
    10441 ttgaagcttg tcgaaaatag gcttcaacaa tcttggcacg acttcgttgt ggatagtcca
    10501 cttgtacgat ttgaatagga gttggtttct tatgtataac agccaagccc tgagacaaat
    10561 agtagtcgtt ggtagcattg atcatctttt caaaagacat tcctcgattt gcgaaatttt
    10621 tgggttgaga aagagatgtt tgtctttttt gtgatgaaac tttatgtgga tagttgacca
    10681 taattctcct tattggtaca ataacatcac tttattatac cataatttgc acagaaaggg
    10741 ttaaaaatgg ctacagcttt ggttttaggc tattctgctt ttgatttggg tttattttct
    10801 gataaggatc ctcgtcttaa attgattaaa aaagcgatac gtaaagattt agaggctatg
    10861 gcagcagatg gggtgtcttg gcttgtgttt acagggagtt tgggttttga atattgggtt
    10921 ttagaggttg ctcaggagat gaaaaccgaa tacggtttcc agttggccac catttttgct
    10981 tttgaaaccc atggggaaaa ttggaatgaa ggcaatcaga tgaaactgag tcgctttaag
    11041 caggtagact ttgtcaaata tgcttatcct cgctatgaac acaaggggca gttaagagat
    11101 taccagcagt ttttgctgga aaacacgact agttcctatc ttttttatga tgaagaaaat
    11161 gaaacaaaac tagcttattt ttaccaaaag atgaaaaatc aagaggacta ttttataaag
    11221 agattaacat ttgatgaatt aaatgaactt gctgaaaatt tttccgaaaa ttgaagcttt
    11281 gaccttgatt tttgtttgcc tttttttata taataatact agcaagcgag aatggagaga
    11341 gacatggcaa gtattatttt ttcagcgaaa gatatttttg aacaagagtt tggacgtgaa
    11401 gtccgtggct ataataaagt agaagttgac gagtttttag acgatgtcat caaggactat
    11461 gaaacctatg ctgccttggt caagtcactt cgtcaggaaa ttgcggattt gaaggaagaa
    11521 ttaactcgta aaccgaaacc ttcaccagtt caagcagaac cccttgaagc ggcaattaca
    11581 agttctatga cgaattttga tattttgaaa cgcctgaata gattggaaaa agaagttttt
    11641 ggtaaacaaa ttttagataa ctcagatttt taagtagtta tttgagatgt gcaatttttg
    11701 gataatcgcg tgaggagaat tgcttctcat gaggaaagtc catgctagca caggctgtga
    11761 tgcctgtagt gtttgtgcta ggcgaaacca taagcctagg gacgagaaat cgttacggca
    11821 gttgaaatgg ctaagtcctt ggataggcca gagtaggctt gaaagtgcca cagtgacgga
    11881 gtctttctgg aaacagagag agtggaacgc ggtaaacccc tcaagctagc aacccaaatt
    11941 ttggtcgggg catggagtac gcggaaacga acgtagtatt ctgactgcta tcagctagag
    12001 ctgttagtgg tagacagatg attatcgaag gaagtggtcc tagtcacttc tggaacaaaa
    12061 catggcttat agaaaattgc atataggttg gggctgagaa attttctcaa cctcattttt
    12121 taaagtggac atatagaaag gtcttgcaag actgtaacat gaaaaaagaa tttaatttaa
    12181 ttgcaactgt ggcagcaggg cttgaggctg tcgttggtcg tgaagtgcga gagttgggct
    12241 acgattgtca ggttgaaaat ggacgtgttc gttttcaagg agacgtgaga gctattatcg
    12301 aaaccaacct ttggcttcgg gcagcagatc gtatcaaaat tatcgtagga acgttcccag
    12361 ctaagacttt tgaagagcta tttcagggag ttttcgcttt agattgggaa aattatttac
    12421 cacttggagc tcggttcccg atttcaaaag ctaaatgtgt taagtccaaa cttcacaatg
    12481 agcccagtgt tcaggctatt tctaagaaag ctgttgtcaa gaaattgcag aaacactatg
    12541 ctcgcccaga aggggttcct ctgatggaga atggcccaga gtttaagatt gaggtctcta
    12601 ttctcaaaga tgtggcaact gtcatgattg atacgaccgg gtctagcctc tttaaacgtg
    12661 gttatcgtac cgaaaaaggt ggcgctccta tcaaggaaaa tatggcagca gccattttac
    12721 aactttctaa ctggtatcca gacaagcctt tgattgatcc gacctgtggt tcggggactt
    12781 tctgtattga ggcagttatg attgctagaa agatggcgcc aggtcttcgt cgctcttttg
    12841 catttgagga atggaactgg atcagcgatc gcttgattca agaagtgcgc acagaagcgg
    12901 ctaaaaaagt agaacgtgag cttgagttgg atatcatggg ctgtgatatt gatgctcgca
    12961 tggtggaaat tgctaaggcc aatgctcagg cagctggtgt tgcaggagac attactttta
    13021 agcagatgcg cgtgcaggat ttacgttccg ataaaatcaa tggagtaatc atttccaatc
    13081 cgccttatgg tgaacgtttg tcagatgatg caggggtgac caagctctat gctgagatgg
    13141 ggcaagtatt tgcaccgctg aaaacttgga gcaaatttat cctgactagt gatgaagctt
    13201 ttgaaagcaa gtatggtagc caagcagata agaagcgtaa gttatacaac ggaaccttga
    13261 aagtggatct atatcaatat tttggtcagc gtgtcaaacg gcaagaggta aaatagaaag
    13321 ggatactcat gagtaaaaaa agacgaaatc gtcataaaaa agaaggtcaa gaaccgcaat
    13381 ttgattttga tgaagcaaaa gagctaacag ttggtcaagc tattcgtaaa aatgaagaag
    13441 tggaagcagg agtcttgcct gaggattcca ttttggacaa gtatgttaag caacacagag
    13501 atgaaattga ggcggataag tttgcgactc gtcaatacaa aaaagaggag ttcgttgaaa
    13561 ctcagagtct ggatgattta attcaagaga tgcgtgaggc tgtagagaag tcagaagctt
    13621 cttcggagga agttccatct tctgaagaca tcttactacc cttgcctctg gacgatgagg
    13681 agcaaggctt ggatcctcta ttgctagatg atgaaaatcc aacagaaatg actgaagaag
    13741 tggaagagga gcaaaacctt tctcgtctgg atcaagagga ctcagaaaag aaaagtaaaa
    13801 aaggctttat tttgaccgtt ttggcgcttg tatcagtaat tatttgtgtc agtgcttatt
    13861 atgtctaccg tcaagtggct cgttcgacca aggaaattga aacttctcaa tcaactacag
    13921 ccaatcaatc agatgtggat gattttaata cactttatga cgccttttac acagatagca
    13981 ataaaacggc tttgaaaaat agccagtttg ataaactgag tcaactcaaa accttgcttg
    14041 ataaactgga aggtagtcgt gaacatacgc ttgccaaatc taaatatgat agtctagcaa
    14101 cgcaaatcaa ggctattcaa gatgtcaatg cacaatttga aaaaccagct attgtggatg
    14161 gtgtgttgga taccaatgcc aaagccaaat cggatgctaa atttacggat attaaaactg
    14221 gaaatacgga gcttgataaa gtgctagata aggctatcag tcttggtaag agccagcaaa
    14281 caagtacttc tagctcaagt tcaagtcaaa ctagcagctc aagctctagt caagcaagtt
    14341 caaatacgac tagtgagcca aaaccaagta gttcaaatga gactagaagt agtcgcagtg
    14401 aagtcaatat gggtctctcg agtgcagggg ttgctgttca aagaagtgcc agtcgtgttg
    14461 cctataatca gtctgctatt gatgatagta ataactctgc ctgggatttt gcggatggtg
    14521 tcttggaaca aattctagcg acttcacgtt cacgtggcta tatcactgga gaccaatata
    14581 tccttgaacg tgtcaatatc gttaacggca atggttatta caacctctat aagccagatg
    14641 gaacctatct ctttaccctt aactgtaaga caggctactt tgtcggaaat ggcgctggtc
    14701 atgcggatga cttggactac taagcagtcg ttacaaaatt ctttcttttc aaaagtaaaa
    14761 atgataaaat aaaacaaatg aaacaagagg agtgtcaaat gacaaaagct aactttggtg
    14821 tcgtaggtat ggccgtaatg ggtcgtaacc ttgcccttaa tattgaatca cgtggttaca
    14881 cagttgctat ctacaaccgt agtaaagaaa aaacggaaga tgtgattgct tgccatcctg
    14941 aaaagaactt tgtaccaagc tatgacgttg aaagttttgt aaactcaatc gaaaaacctc
    15001 gtcgtatcat gctgatggtt caagctggac ctggtacaga tgctactatc caagcccttc
    15061 ttccacacct tgacaagggt gatatcttga ttgacggtgg aaatactttc tacaaagata
    15121 ccatccgtcg taatgaagaa ttggcaaact caggtatcaa ctttatcggt actggagttt
    15181 ctggtggtga aaaaggtgcc cttgaaggtc cttctatcat gcctggtgga caaaaagagg
    15241 cctacgaatt ggttgcggat gttcttgaag aaatctcagc taaagcacca gaagatggca
    15301 agccatgtgt gacttacatc ggtcctgatg gagctggtca ctatgtgaaa atggttcaca
    15361 atggtattga gtacggtgat atgcaattga tcgcagaaag ctatgacttg atgcaacact
    15421 tgctaggcct ttctgcagag gatatggctg aaatctttac tgagtggaac aagggtgaat
    15481 tagacagcta cttgatcgaa atcacagctg atatcttgag ccgtaaagac gatgaaggcc
    15541 aagatggacc aatcgtagac tacatccttg atgctgcagg taacaaggga actggtaaat
    15601 ggacgagcca atcatctctt gaccttggtg taccattgtc actgattact gagtcagtgt
    15661 ttgcacgcta catttcaact tacaaggaag aacgtgtaca tgctagcaag gtgcttccaa
    15721 aaccagctgc cttcaacttt gaaggagaca aggctgaatt gattgaaaaa atccgtcaag
    15781 ccctttactt ctcaaaaatc atttcatacg cacaaggatt tgctcaattg cgtgtagcct
    15841 ctaaagaaaa caactggaac ttgccatttg cagatatcgc atctatctgg cgtgatggct
    15901 gtatcatccg ttctcgtttc ttgcaaaaga ttacagatgc ttacaaccgc gatgcagatc
    15961 ttgccaacct tcttttggac gagtacttct tggatgttac tgctaagtat caacaagcag
    16021 tgcgtgatat cgtagctctt gcggttcaag ctggtgtgcc agtgccaact ttctcagcag
    16081 ctattactta ctttgatagc taccgttcag ctgaccttcc agctaacttg atccaagcac
    16141 aacgtgacta ctttggtgct cacacttacc aacgtaaaga caaagaagga accttccact
    16201 actcttggta tgacgaaaaa taagtaggtc agccatgggg aaacggattt tattacttga
    16261 gaaagaacga aatctagctc attttttaag tttggaactc cagaaagagc agtatcgggt
    16321 tgatctggta gaggaggggc aaaaagccct ctccatggct cttcagacag actatgattt
    16381 gatgttattg aacgttaatc tgggagatat gatggctcag gattttgcag aaaaattgag
    16441 tcgaactaaa cctgcctcag tcatcatgat tttagatcat tgggaagact tgcaagaaga
    16501 gctggaagtt gttcagcgtt ttgcagtttc atacatttat aagccagtcc ttatcgaaaa
    16561 tctggtagcg cgtatttcgg cgatcttccg aggtcgggac ttcattgatc aacactgcag
    16621 tctgatgaaa gttccaagga cctaccgcaa tcttaggata gatgttgaac atcacacggt
    16681 ttatcgtggt gaagagatga ttgctctgac ccgccgtgag tatgaccttt tggcgacact
    16741 tatgggaagc aagaaagtat tgactcgtga gcaattgttg gaaagtgttt ggaagtatga
    16801 aagtgcgacc gagacaaata tcgtagatgt ctatatccgc tatctacgga gcaagcttga
    16861 tgttaaaggc caaaaaagct acattaaaac cgtgcgtggt gttggatata ccatgcaaga
    16921 atagataagc agttgcagtt gtgtaactgc ttttttgagg agtttttata tattgacatg
    16981 cagtcgggtc tttgctacaa tcgttttagg aggatatata gatgaaactt ttgaaaaaaa
    17041 tgatgcaagt tctactagca gtctttttct ttggtttgct agctacaaat acggtatttg
    17101 cgaataccac aggtggccga tttgttgata aggataatag aaaatattat gtaaaagatg
    17161 atcataaagc aatctattgg cataaaatag acggtaaaac ttactatttt ggtgatattg
    17221 gagagatggt tgtcggttgg caatacttag aaattcctgg aacaggttat cgtgataatt
    17281 tattcgataa ccaaccagtt aatgaaattg gccttcagga gaagtggtac tattttggac
    17341 aagatggtgc tttgctagaa caaacagata aacaagtact agaggcaaaa acgtctgaaa
    17401 atacaggaaa agtatacggt gaacaatatc ctctatctgc tgaaaagaga acttattatt
    17461 ttgataataa ttatgctgta aagacaggct ggatttatga agacggcaat tggtattatt
    17521 taaataagct aggaaatttt ggcgatgatt cttacaatcc actaccaatt agtgaagttg
    17581 ctaagggttg gactcaagat tttcatgtta ctattgacat tgatagaagc aaacctgctc
    17641 catggtacta cctagaccca gaaactggca ttatgcgaac aggatggcaa tatctaggta
    17701 ataactggta ctacctccgt tcatcaggag caatggccac tggctggtat caggaaggta
    17761 ccacttggta ttatttagac cagccaaatg gcgatatgaa aacaggttgg caaaaccttg
    17821 ggaacaaatg gtactatctc cgttcatcag gagctatggc aactggttgg tatcaagatg
    17881 gttcaacttg gtactaccta aatgcaggta atggagacat gaagacaggt tggttccagg
    17941 tcaatggcaa ctggtactat gcttatagct caggtgcttt ggcagtgaat acgaccgtag
    18001 atggctattc tgtcaactat aatggcgaat gggttcggta atgacagggc gcttataatt
    18061 atattagaac actcgcttaa tcaaagaagt gactttgttg tacttgacct gacgattggg
    18121 gagattaaaa gcatgtgaaa tctacacatg ctttttcgtt agttatgagt gttgaacttg
    18181 actaaccatt gacaaaagat aagagcaatt aaaataagat acaaaaatat caatgctaac
    18241 tgcaccgaaa cgcccgatgc tatggatagg gctaataggg agagcaggga cggggcgaga
    18301 atgtccactg tgctcagagc agagcccacc gtccccatct tgtcactggg gatataggca
    18361 aagacagctg cttgtaggcg agggctgaga atgccgataa tcgtagaact gatgaaactg
    18421 gtcatgaaaa ttacgatata ggcttgacaa aggatactag ttattagagc caatagggaa
    18481 atctcacaga aaaccaagag gtgcgtgaaa cggatatttt taaatagatt actgctggtc
    18541 atattgccaa ggatagctcc acaagacatg atgatggaga gcagggcgag gagttgccca
    18601 atgctcagtt gtgctagtgt ctgttggatg aagcggaggg caatcagccg aggggacaca
    18661 tccaagatgg caatcatact ggtcgtcaga aacagtagta tgacggtatc ttttaacttc
    18721 agtagcacag ccagcgactg tttaagatgt tgaaaatatt tcttaaaact cagtgctgta
    18781 tgtgacattt caatcctttt ttcaacctca tacaaggtat gtcgaataac atacaggccc
    18841 aaaaaggcaa tgacaaaagt cagagtgttg ataagggaaa tagtttgaat acttataaca
    18901 ttgataagga atgcgccagc cagattggcg acaatgcgga caaccctcat cagagactgc
    18961 ctgaacccca tggcatcatg caggtcgtcc ttaattacac tgatgtagag ggcgttcatc
    19021 atgtaggcag acaggtagct caaggtgtca gagatgaggt tgatgatgac aatcataatg
    19081 actgaaacta atgaaaaggg cgacaagacg agaaatactg tcaaaatagc gtagagcagg
    19141 attttgattt tggcaataca gagtgcgtgt ttgactctat tttcttgaaa atctcctaga
    19201 atcccgagaa agagttgcga tagtagggga accatctctg aaatggaaat cagtaaaatc
    19261 gctagcggag cgaaattgta ctgagccaca taattgataa tggcgaggta gaaaatactg
    19321 tcgccgaatg atgaaaaaca gctgtttagc agcaactgtc tatatatgtt gtttgtccaa
    19381 aataatttca tacatttctc cttatgtctg cacatcgaat gggaatttat tatttaatag
    19441 acaccaatat acccccatgc ttcctattcc gaagtcaaac aaatctgctt taccactatc
    19501 tccttgaaac atcagtaaac cgtgatcatc tcgttcagaa catgctaaaa ttacctgttg
    19561 gattttttca tcagtaattt gttttgattg tagacagtgg tttgtagtaa gctagttatt
    19621 ccgtggcaat atcctgtcat catttttaga tgatggttaa aaacaaattc ttgatattta
    19681 ctaataatat ctttgtttcc gtcacaatcg tacatacaaa gatataagat tattccggaa
    19741 attcctttgc accaagaaag ttgtaaattt cctaaatttt cagtattaga agtaactttt
    19801 tctaataatt tttttaattc agtatggaat gtatggagat cattatagaa catagaaggt
    19861 tctaagactt tagaataggc aaataaagca tatgcaattc cacaatatcc atgagcaaag
    19921 ctatagtcta aaatgctttc tttggctgta tctctttgta ggaaataatg ataaatttcc
    19981 ccatatacta ttgtggatga aagttttaag agtattgtca ttggtaactt gatagtattt
    20041 tattaatgat aataggacac cagattttcc caatgcaaag tctatttcct ctagagtatc
    20101 ataattctct attaatttct tagcggtgac gtttgctaat tgtagatagt attgattttt
    20161 actgatagaa aataaatgta ggagtaacta tagatagcct gcaccaccaa atagcagtga
    20221 gtattgatat tcatatgtgt gtgacgttct aattgtcact gccttcttga tgatagagag
    20281 tattttagaa tccagttctg ggtggtacaa tttatttaga ggaaacagta gtccagctat
    20341 tccgtgttgg aatgataaat tacttacgaa atttccaaat tctgttgaag aagtgatcct
    20401 tccttcagaa tttacagttt tatctagtaa aaatcgtaga acactattac attctgtttt
    20461 gtaatcatat ttatgttcct ctattttttt aattttgact aaattatttg tttgacttaa
    20521 atttagtaga cattgtaacc aaaatagttg ctcatctgtg agctgacctt gagtatgtga
    20581 tagttctagt aattgaatag gatcctttag ttagattcaa tcaatctata acacaacaac
    20641 ataataatac ttttaggata acttgtcaaa ta
//
LOCUS       DAVBNO010000034        20031 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVBNO010000034 DAVBNO010000000
VERSION     DAVBNO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.034"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(112..387)
                     /locus_tag="VQ846_001361"
     CDS             complement(112..387)
                     /locus_tag="VQ846_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET3075936.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(494..1333)
                     /locus_tag="VQ846_001362"
     CDS             complement(494..1333)
                     /locus_tag="VQ846_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET3075937.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILCHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1475..2323
                     /locus_tag="VQ846_001363"
     CDS             1475..2323
                     /locus_tag="VQ846_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET3075938.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2416..3333)
                     /locus_tag="VQ846_001364"
     CDS             complement(2416..3333)
                     /locus_tag="VQ846_001364"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET3075939.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRIIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3770..3975
                     /locus_tag="VQ846_001365"
                     /pseudo
     CDS             3770..3975
                     /locus_tag="VQ846_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4286..4579)
                     /gene="rpmA"
                     /locus_tag="VQ846_001366"
     CDS             complement(4286..4579)
                     /gene="rpmA"
                     /locus_tag="VQ846_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET3075940.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4596..4940)
                     /locus_tag="VQ846_001367"
     CDS             complement(4596..4940)
                     /locus_tag="VQ846_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET3075941.1"
                     /translation="MIQAVFERAEDGELRGAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(4956..5270)
                     /gene="rplU"
                     /locus_tag="VQ846_001368"
     CDS             complement(4956..5270)
                     /gene="rplU"
                     /locus_tag="VQ846_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET3075942.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5306..5393)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5488..6087)
                     /locus_tag="VQ846_001369"
     CDS             complement(5488..6087)
                     /locus_tag="VQ846_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET3075943.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLF
                     SSFVLGVVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            6557..6856
                     /locus_tag="VQ846_001370"
     CDS             6557..6856
                     /locus_tag="VQ846_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET3075944.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            7075..8152
                     /locus_tag="VQ846_001371"
                     /pseudo
     CDS             7075..8152
                     /locus_tag="VQ846_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8468..9442)
                     /gene="pta"
                     /locus_tag="VQ846_001372"
     CDS             complement(8468..9442)
                     /gene="pta"
                     /locus_tag="VQ846_001372"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET3075945.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(9486..10382)
                     /locus_tag="VQ846_001373"
     CDS             complement(9486..10382)
                     /locus_tag="VQ846_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET3075946.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(10379..11197)
                     /locus_tag="VQ846_001374"
     CDS             complement(10379..11197)
                     /locus_tag="VQ846_001374"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET3075947.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(11181..11852)
                     /locus_tag="VQ846_001375"
     CDS             complement(11181..11852)
                     /locus_tag="VQ846_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET3075948.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            11980..12549
                     /locus_tag="VQ846_001376"
     CDS             11980..12549
                     /locus_tag="VQ846_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET3075949.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            12662..13621
                     /locus_tag="VQ846_001377"
     CDS             12662..13621
                     /locus_tag="VQ846_001377"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET3075950.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            13631..14746
                     /locus_tag="VQ846_001378"
     CDS             13631..14746
                     /locus_tag="VQ846_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET3075951.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFTNNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            14751..15098
                     /locus_tag="VQ846_001379"
     CDS             14751..15098
                     /locus_tag="VQ846_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET3075952.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKEVTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            15106..15321
                     /locus_tag="VQ846_001380"
     CDS             15106..15321
                     /locus_tag="VQ846_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET3075953.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            15404..16045
                     /locus_tag="VQ846_001381"
     CDS             15404..16045
                     /locus_tag="VQ846_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET3075954.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            16063..16752
                     /locus_tag="VQ846_001382"
     CDS             16063..16752
                     /locus_tag="VQ846_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET3075955.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(16749..17429)
                     /gene="radC"
                     /locus_tag="VQ846_001383"
     CDS             complement(16749..17429)
                     /gene="radC"
                     /locus_tag="VQ846_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET3075956.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(17472..19763)
                     /gene="pcrA"
                     /locus_tag="VQ846_001384"
     CDS             complement(17472..19763)
                     /gene="pcrA"
                     /locus_tag="VQ846_001384"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET3075957.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQNCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGPGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDTDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
BASE COUNT         6200 a         4213 c         3617 g         6001 t
ORIGIN      
        1 aatattttcg gaacattcaa ctttttaact ctatttatta ctagatttca taattaaaaa
       61 gcctactgac caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac
      121 agcgtcttta agagctttac cagctttgaa tgctggtact ttagaagctg caattgtcat
      181 ttctttacca gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt
      241 accaaaaccg atcaactgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa
      301 tacagcttca actgctgctg ctgagtcttt cttagtcaat tctgtagctt ctgctacttt
      361 agcgatcaaa tcttgtttgt ttgccatgtt aatgattcct ccaaataatt tctaattaac
      421 aaatataatc atatcctaaa atccctttta ggtcaagtta aaaacgctat ttcttcccat
      481 tttatttatt tttttaggag tggtaacgta tcaaaatagc ccaagcgttc tcacccgtgt
      541 gagtttgaat aatggaaccc gtttccaaaa cagaaattgg cttttcaaca taagcttgta
      601 agctttcttt catctctttt gcccaatcat cactaccaga atatgaaatt ccaatctctg
      661 ctacagcacg ttcagaaagc gatgttatca actcatctaa ccatttttta aatgttttag
      721 ttccacgacc tttaaccatt ggctgcaatt catggtcttt catttgcatg acaacacgga
      781 tattgagaag agagctcaac aagccagtta cacggctaat tcgtccacct ttgacaagat
      841 tttccaaagt tgaaacacca atataaagct ctgtatggtt tttaacctct tctacatgac
      901 ataaaattgc ctccatatct ttaccttctt gagctaactt cgcagcctca acaacttgga
      961 atttcagggc ttggtcagtg aaggaactat caacaacagt cacatctgca gtagataggc
     1021 tagcaccttg gcgtgctgct tctaccgtac ccgaaagagc atgggacata tgaatagcaa
     1081 gaatctggcc accatctttg cataggtctt caaaaatctc agcaaagaca cctacaggtg
     1141 gctgacttgt tttcggaaga ttcttacttt cttgcatcaa ctgaagaaat ttaccttctt
     1201 ctttcaaatc cgcatcagaa taaacaacat tatcaatcat tacagataat ggaacaattg
     1261 taatatctaa ttgctttact agttcaggtt caatagtaac agatgaatcg gttacaatct
     1321 taatttttgt catagtatca atctttctat tttaggattc agattggttt ccttacttct
     1381 aattatatca aaaaaaagat taaaaatcct aatggagtca atcaaatttt ccgtaaaatt
     1441 tgatataatc aacttataag aaaagaggtg tcctatgatt aaaaaaattt accccatttt
     1501 taccatttta ctaggtgctg ctatttatgc ttttggactg acttattttg tagttcccca
     1561 tcatctcttt gaaggagggg cgacaggtat taccctcatc acattttatc tttttaaaat
     1621 ccctgtttcc ctcatgaacc tgctgattaa tattcccctt ttcatcctag cttggaaaat
     1681 ctttggagcc aaatccctct attctagttt actaggaacc ttagctttgt ccggctggtt
     1741 agcttttttt gagcatattc cccttcatat tgatcttcaa ggtgatttac taatcacagc
     1801 ccttatagcg ggaatcctat tgggaattgg ccttggaatt atttttaatg ctggaggtac
     1861 aactggtgga actgatattc tagctcgtat tctcaacaaa tacactcata tatccatagg
     1921 aaaactgctc tttatcttag atttttgtat tctcatgttg attctcctaa tcttcaagga
     1981 tttgagattg gtttcctaca cgcttttgtt tgattttatt gtttctcgtg ttattgattt
     2041 gattggtgaa ggaggatatg ccggcaaagg ctttatgatt atcacaaaac gtcctgacca
     2101 acttgctaag gcgattaatg atgacctcgg aagaggtgtt acttttattt ctggtcaagg
     2161 ctactatagt aaagaaaatt tgaaaatcat ctactgtatt gtcggaagaa atgaaattgt
     2221 gaaaacgaag gaaatgattc atcgaatcga tcctcaagcc tttataacta ttacagaagc
     2281 ccatgaaatc ctaggagaag gcttcacctt tgaaaaagaa taaaaagagg taatgtcgtg
     2341 acctcaaaag ttagactaaa tcatctatct tttgggttac agacaacctc ttttttgttt
     2401 tatttactca agctcttaag accaattccg agttacttct tcatcagcct ttaactgatc
     2461 cactaattgg tcaactgagt caaatttggt catatcacga atgcgatcaa gccaataaac
     2521 catgacggtt tccccataaa tatcttgatt aaaatcaaaa atattgactt caaaacgtgc
     2581 ttcttctcca tcaaaggtca catttttccc gacactagcc atagcacgat acttctgtct
     2641 ttgaatctca acatcaacga cataaacgcc atctgctggc atataagtac ggtctaaaag
     2701 cactaaattc gctgtcggat aaccaattat acgaccacga gcattaccat gaaccaccat
     2761 acctcttgat ggaagcggtg cccccaaaag ttttcctgct tctttcacat ttccatctaa
     2821 aatagcttga cggatacgag ttgaactaat ctttcctttc tcatcttcta caggtggaac
     2881 aatgataact tctccatcaa agtaatcctt taaatcttct gctgtttttt tgtcagaacc
     2941 aaatgtataa tcaaaacctg caacaataat tttggcattc atagccttga tataagttgc
     3001 aaagaattct tgtgcagtga gactagcgaa ttgactacta aaatcaagga gatataattc
     3061 ttctacacct tcgcgcttta attttctttc acgttcagca gggttcaaaa tatgcaaaaa
     3121 caaatccgga tgataaggct ctaaagcgat ctttggagat tcattaaagg tcataacgac
     3181 gataggtaat aaatcctttc tcgcagcctt gttggcaaca cgaaataatt cttgatgccc
     3241 cttatgtatg ccatcaaaat agccgagaac aacgactgaa tcagatggtg tgccaatatc
     3301 tttttggttt tttataggaa tagtaataat cataaaataa ttatatcata gcgatagcta
     3361 tttctggaac agaaaatctg aaatgttgtt tttttcacat gaagtgtacc tgttttcaaa
     3421 aagcacttta ttctatcgtt gcttaactat gaactttgca atattcttct caaaaacttg
     3481 taggacatct tcaaaatttt gcaaggagtg attagacttg ttcggtaacc ataaagtgtc
     3541 atactatgtt atgtatgaaa aagcaatgca actaactcct gagaacttta aattactaat
     3601 tggtgccgaa aaggtagaat ttagaatcga ggtacaccta tggctgtaaa atttacaaaa
     3661 tgagacaact tgggcaagat gtttgaagaa tttcctaaac tccctgattt gaagcaagtc
     3721 actttccctg atgacaaaga aaaaagccaa aacagcaaag aaaaactaga tgactgcttt
     3781 ccaacaactc ccatctagtg tgcttcagac tgggctattt ttctctccat ctgttagctt
     3841 ggattctcag accgtttcag ctaaagaata tcttttccct tatcagaagg aacggctcaa
     3901 accattcaga caagtgaagg gacgacaagc caatatttga aaccagatag cagttcttat
     3961 agtcaattga aataaaatct gaagaaatcg agtaggaaac tcatatcaat ttttaacagt
     4021 gttctattcc agattcatac tcaatgaaaa ttaaagtgca aactaggaag ttagccgcag
     4081 gctgtacttg agtacggcaa ggtgaaactg acgtggtttg aatttgattt tcgaagagta
     4141 tcaatccact atacatatta aaaagtatgg atatcaaaac aaaaaaacag gctctccgaa
     4201 aactcggaaa gccttatttt aagctgtttc tagcttcctc acctttacat ttcaaggttc
     4261 gggataaaag gttcaatgga cctttttatt tagcgattgg gtaaacagaa acttgttttt
     4321 tatcgcgtcc tttacgttca aagcgtacta cgccttcaac tttagcgaac aaagtatcgt
     4381 ctccaccacg accaacgttt acacctggat agatgtgtgt accacgttga cggtaaagga
     4441 ttgatccacc tgttacagtt tgtccgtcag ctgctttagc tccaagacgt tttgcttgtg
     4501 aatcacgtcc gtttgatgta gaacctccac cttttttgtg ggcgaaaagt tgcaagttgt
     4561 taagagtcat ttttaacata atgttttcct ccgtgttagt tttctgtgat aactctggtt
     4621 tggacgaact cagaatagtt ctccgataag tttgccatac ctaagaaaaa tgattcaaag
     4681 aataactggg tcatttctct ctggtgtgaa ggaagatctt ttggtatttc aaccatcaga
     4741 tagccacctt catcttcgtt taattctagg attggttcat agcctgcaaa tttctcaata
     4801 gaattgataa agttaatggc aagcgtagaa accgatgcac acacgacatc taagccgtat
     4861 tcgccactct cggcgtgtcc agtaatttcc gcacccctca gctcgccatc ttcggctctc
     4921 tcaaagactg cttgtatcat gtgttctcct taaaattaag cgttaattgc gttgatgaca
     4981 acttttgtat atggttgacg gtgaccttgt ttacggtggc tacctttttt aggtttgtac
     5041 ttgtaagtaa caactttctt ttgttttcct tgtttttcaa cagttccaac tacagtagct
     5101 ccagcaacaa gtggagttcc gacaacagtg ttttcaccac caacaagaac aacttcgtta
     5161 aaagtaactt cttgaccagc ttcaacgttc aatttttcaa tgtaaactgc ttgaccaact
     5221 tcaactttaa cttgttttcc gccagttttg ataattgcgt atgtgctcat tatgcacctc
     5281 ctatgatttt tatgggtttc cccgattttt tgtgaagact cgcctagcat cgtgggacaa
     5341 accacttaaa cttgaataat caagcaacga tgttcgtgcg gttgcacagg atcgtgcata
     5401 gtcaactctt caagtatagc atatctccta ttttcttaca agtaataaca cctaaaatca
     5461 agctttttct tttacttttt tctgccacta ggcaaaaagc atactgagat aaaaaatgct
     5521 catcataata ggaacaccaa gaatggtctt ttcatgatag aaaatcgtca aataggctga
     5581 aaagacaacg ccaaggacaa aactactaaa caggctaaca aatatgaacc cttcacgcaa
     5641 aaaaggagtg tgcttagttc ggaaataatc tccaaaagct agcattgttc gtttgatatt
     5701 ccctgtcata aaggcattat tgtaggcaat acccgaaacc tcaccaaaag cagttgtcac
     5761 cagtcccata cagaaggcca agggctgcac tagatagata ttatctacag tttgtggcac
     5821 aaaaccaata attattgata ggattgctaa aggaatcaag gacagaatag gttttttcac
     5881 aattctcaat ttttccttat aaatcgttaa taaaaagact cccattataa acgttattaa
     5941 ggttaaaagc ttatccctaa catccgaaac attattttgt attagctcta ccgaaagaaa
     6001 gacaacattt ccagcttgtc cagctacaag ggtattccct cgaacaataa aagtgtaagc
     6061 atccacatat ccagcacaaa atgtcaaaaa agtgctaacc ttttagactg acgtgatatt
     6121 tttcttatag gtaataacct cattttccct cccattgtat tttctcttag aaatattgta
     6181 ccatttcttt ctaaaaaatc gtagactacc atttagattt tactattagc ataaaaataa
     6241 taatagacaa ctatttatcc aaaaatagat agaggtaaca tgtttgcaaa caaagcatac
     6301 gaacctttag taaaatcatt tccatgaaac tagaatagag cccctcttag caaaaatcat
     6361 tattttaatt tatttctaat cactccttga cataaataac tctcaccaat aaaagactat
     6421 gtcttaaaaa aatggtataa taaaatcaat acctgggctt gatggctatg ctactaataa
     6481 caattaggag agaaaatcag gcacttgtta acaacaagga ttatcccctt gagatgaaag
     6541 gaactttaga aatcttatga tgaacatgca aaacatgatg cgtcaagcac aaaaacttca
     6601 aaaacaaatg gaacaaagcc aagctgaact tgctgctatg caatttgttg gcaaatctgc
     6661 tcaagatctt gtccaagcga ccttaactgg cgataagaaa gttgtcagca ttgatttcaa
     6721 tccagctgtc gttgacccag aggaccttga gactctttct gatatgaccg ttcaagccat
     6781 caactctgct cttgaacaaa tcgatgaaac tactaagaaa aaactgggtg ctttcgctgg
     6841 gaaattgcca ttctaaaaac aaggagctag aacaatactt gtcgataaca aaggctaaga
     6901 aaggtgcaaa aattataaaa aacgcatagt atcaagcaac ctaaaacttt gggctctata
     6961 atatttgtag tgggtaaatc cactatagat attatggagc ctattttatt gtagaaaaaa
     7021 atcccataag atctataatg aaaagcgacc aaacaactca ttagaaagat tcatatggga
     7081 caattacatt ttatcacaaa attactagac attaaagacc ctaatatcca gattttagac
     7141 atcatcaata aggatacaca caaggaaatc atcgccaaac tggactacga cgccccatct
     7201 tgccctgagt gcggaagtca aatgaagaaa tatgactttc aaaaaccgtc taagattcct
     7261 taccttgaga caactggtat gcctactaga atcctcctta gaaagcgtcg attcaagtgc
     7321 tatcactgtt caaaaatgat ggtcgctgaa acttctatcg tcaagaagaa tcaccaaatc
     7381 cctcgtatca tcaaccaaaa gattgctcaa aagttaattg aaaagatttc tatgactgat
     7441 attgcccatc agctttccat ctcaacttca actgttattc gtaagctcaa tgattttcac
     7501 tttgagcatg atttttcgcg tcttcctgag attatgtctt gggacgttga aacagtccgg
     7561 ggagtgactg tttcaatcgg gagatggaga tgagctttat tgctcaggac tttgataagc
     7621 tcaatatcat aactgttctt gagagcagaa cacaagccat catccgaaat cccatgaata
     7681 caaggctatc aagcgctact ggaaactcat tcaacaggat agccgtaaac tgagtgataa
     7741 gcgattttat cgtcctactt ttcgtatgca tttaaccaat aaagagattt tagacaagct
     7801 tttgagctat tcacaagact tgaaacatca ctatcagctc tatcaactct tgctgtttca
     7861 ctttcagaat aaggaaccgg agaaattttt cggactcatt gaggacaatc taaagcaggt
     7921 tcatcctctt tttcagactg tctttaaaac ctttctaaag gacaaagaga aaatcgtcaa
     7981 cgcccttcaa ctaccctatt caaacgccaa attggaagcg accaataatc tcatcaaact
     8041 tatcaagcgc aatgcctttg gttttcggaa ctttgaaaac ttcaaaaaac ggatttttac
     8101 cgctctgaat atcaaaaaag aaaggacaaa atttgtcctt tctcgagctt agcttttctt
     8161 caacccacta cagttgacaa agagccagaa aacgcataat agcaggtgtc aagacactta
     8221 atattatgcg ttttctcatg aaaaaattct cttttcatac tcatttaaaa tcaaaaaggg
     8281 aaattatcgt tttacggaca tacatattac agatttctat aggtcgtgta tagacaggat
     8341 atcatcgctt ggaagttttc ctttttgaca ccaatcagta atttagttct caggaattaa
     8401 tggcagtgct tttcgtacat agtttcataa ctacagtata gcatagtata tcgctttata
     8461 gttttcacta ttgatgaact gcttgagctg ctgtgatgag ggtcaacttg taaacatcat
     8521 ctgcattaca tccacgagaa agatcattaa ctggcttgtt taaaccttgc aaaacaggtc
     8581 ctacagccgc aaagccaccc aggcgttcag ccatcttgta accgatattt ccggcctcga
     8641 tacctgggaa gatgaagaca tttgcttgac cagctaccgt acttccagga gctttcagag
     8701 ctgcagtttc aggaacaaag gctgcatcaa attgcaactc accatcgatt tcaaggtcag
     8761 gacgcaagtc gtgagcaatt ttagttgctt caacgacctt atcaacgctt tcaccaaacc
     8821 ctgaaccttt agtagaatag ctcaacatgg caattttagg ttcgatgcca aacatcttag
     8881 ctgtgattgc tgagttgatg gcaatttcag ccaaggcttc tgcatctgga ttgatgttaa
     8941 tggcacagtc tccaaatagg taacgttccg taccacgaac catgaggaag gctcctgaag
     9001 tacgagttac atttggacga gttttgatga tttgtagagc tggacgaact gttgaagctg
     9061 ttgagtgaat cgctcctgac accattccat caaccaagcc caagtaaacc aacatcacac
     9121 caaagtagtt gacatcttca accaaaacct tgcgtacatc ttcttcagtc attttgccct
     9181 tgcgacgctc caccaaggca gaaaccattt cttcaaattg aggataatgt tgagggtcga
     9241 tgacctcata accatccatg attccttcaa tttcaagata aattttaatt ttttcaggat
     9301 ttccaagcaa aacaggaatc acttctgttt cttttactaa gcgttttgtt gcttgaagaa
     9361 tacgaggctc ttccccttca gggagaacga tacgagcatt tttaccaaca aggttggctt
     9421 tgagactttc aaaaacttcc atgagttttc tcctttaaga taataattat actctgagac
     9481 agtttttata gagtattagt tgataactgg gtaataaggt tactgaaatc atccggcaag
     9541 ggactttcta actgcaagtc ttgctctaaa aatggatgat aaaaggatag gtaatggcaa
     9601 tgcagagcct gacgttgaat accatcttcc agactaccac catacaaatc atctcccagc
     9661 aaaggaaaac cgatatgaga aaaatggact cggatttgat gggttcgacc agtgtgcagg
     9721 tgaatataga ccaagtgaat atttccataa gaagctacaa tcttgtatga agtatgggca
     9781 tactttccgc ctttagccac tcgtctggta ataatggaat cttcatcacg cgcaatcgga
     9841 gcaataattt ccccttctgg ctccaaatgt ccatctccct taaccaaagc aaagtagcgt
     9901 ttctcgatag atttcttctg caactgcttg tctaatcgtg catgggcata accgtgcttg
     9961 gcaaagagca tcaagccaga agtgtcccta tctagtctgg taacaatgtg aacctgctga
    10021 ttttcataat tttgcttgac atagtaaccc ttgataaaat tggcaatggt gttagagtga
    10081 ttgacactag gaatagaagc cactccatag ggtttattca agactagaaa gtggtcatcc
    10141 tcatagagaa tatctaatgg atgctcaata gcctccaagg tttcaaagcc ttcctcagcg
    10201 ggaatgtaaa tggtaacgta gtctccaacg tccaatagat acgttgcatt ttgcggttga
    10261 ttattgacca gaatagctcc acctcgaaac ttaatcttag ccagcaatcc cttagaaacc
    10321 tcgtgctttt ttaaaaaggt cttaaccttg acatgttcat ctgcgataaa ttcaaacctc
    10381 attcatccac ctcaccgata aaggcatcct taacacggtt ccagaaactg gtatggctag
    10441 gagtcgcgac aaagtgaatc ttatgatggt cgatttgata ctcaatacgc tcaatattac
    10501 ggaaagaata aacgctattg tcaaccgaaa tagtatgata atcgtttctt gttggaataa
    10561 gttcaatctt atccttctta ggcacaataa tggaagagcc cagtgttcga taaacacgat
    10621 tattaaggct ggcaatttcc gttaattgca aagcttcaat ggtagggtgt aaaacagcac
    10681 cgccaagaga cttgttatag gcagtactac cagtcggtgt cgaaactgtt agcccgtctc
    10741 cacgaaaacg ttcaaaggga acaccattta ttacaatatc tgccaccatg gttcgatcag
    10801 acctgcggat gctggcttcg ttgagtgctc tgaaaatctt aacttcacca ttttcaagaa
    10861 agaccttcac attcagaaca gggtaagaaa cccttgcccc agtatctagc tgcaaattag
    10921 tcactagctt gtccaactca aaatcacgat aatctgtata gaagcccaaa tgtccagtat
    10981 gaagaccgat aaagcggacc ttgtcaagct gattttcgta cttatgaaag gccgacaaga
    11041 gcataccatc cccgccaatg gaaatgacaa tatccggatt ggtatcattg agtataaact
    11101 gatttctctt caaacgatct cgcaattcat acaaaaccct ttgactctgc ggttttctat
    11161 tggctatcag atcaattcgt ttacctgtat tcttcatctg tatcgtcact gtttcctaca
    11221 ccgtcattta attttctact caaaggatca aaaagtgcct gggcttcttg gatatcatca
    11281 cgaatttcac ccatttcttc atccaactga tgggcgattc tagctgtaat ttccagtcgc
    11341 ttcttaatct catctgggaa atccccttgg tacttgtagt tgagagaatg ttctatcgtt
    11401 gcccagaaat tcatggccaa agtacgaatt tgaatttctg ccaaaatagt cttagctcca
    11461 ttgatggtat caaccgtata ttctactacc acatgatagg aacgatagcc tgatgctttt
    11521 ctatgagtaa tgtaatctcg ctcctgtatg attcgcatat cctgacgctt gtgcaaaata
    11581 tccactactt ccttgacgtc atctacaaac tgaaccatca cacgtaagcc agcaatatcc
    11641 tgcaaatcgt gttccaaggt cgcataagta atgccacgac gagccatttt ttctttgatg
    11701 ctctcaattg gcttgactcg accggtcaca aactcaattg gagaatgctt attttgctta
    11761 cgatattgct tacgaatacc acgaagttta atctttaact caccaacagc ttgaatgtaa
    11821 ggatctagaa attcttccca ttctaaggtc atatattctc ccttgttctc atattatccg
    11881 tcaaaaatat ctttgatttt ttctccagat tcatatacaa taaatacaat gcttattata
    11941 ccataaatcc gctttcaacg ataaaaaaag gtaagaaaaa tgaaacattt agaaattgaa
    12001 ttgaaaacac tattgaaaaa agatgaatac aatcgtctaa aagaccagtt cacaggtgtc
    12061 actcctgttc ttcaaacaaa ttactacatc gacacgcctg attttgaact gcgagaaaag
    12121 aaagtcgcta tgcgcattcg aacctttgaa gactgggctg agttgacact caaagtcccg
    12181 cagagtgttg gaaacatgga atacaaccaa aaattgcaac taaaagatgc tgaggactat
    12241 ctgagtaagg aagaacttcc tcaaggacta gtactggatg aattggcgaa acatggtatc
    12301 caaagtaaga actggcaggt tcttggttgt ctaacaacgc ttcgctatga aatgaaaaca
    12361 gctattggtc tcatggcact ggatcagagc cgatactttg atataacaga ttacgaattg
    12421 gagcttgaag tggaaaatca tgagcaaggc aaacaggatt tccgacaatt tttagagaaa
    12481 aatcagattt cctaccaaaa agctccttca aaattggttc gatttgtcaa aagtatgaaa
    12541 aatagctgaa ataatctcca ttttttggta aaatagaaaa gataaaaata cacaaatccc
    12601 agttcatata tgtaaggtaa atataactag gatttataaa gtttacagag gacggtctat
    12661 aatgtcagat agaaaaaaca tgaaactttt cgcactcaac tctaaccaag agattgcaca
    12721 aaaaattgcc caagctgttg gtgtcccact tggaaaacta tcatcacgtc aattttcaga
    12781 cggagaaatc caagtaaata tcgaagaaag tgtccgtggt tatgatgttt acatcatcca
    12841 atcaacaagt ttccctgtca acaaccacct aatggaattg ttaatcatgg tcgatgcttg
    12901 tgtgcgtgca agtgcccaca gtatcaacgt tgtccttcca tattttggct atgcacgtca
    12961 agaccgcatt gcttgtcctc gtgagccact tacagcaaaa ctagttgcca atatgctggt
    13021 taaggctgga gttgatcgta tcctgactct tgatttgcat gccgttcagg ttcaaggttt
    13081 ctttgatatt ccagtggata atcttttcac tgttccccta ttcgcaaaac attactgcga
    13141 taaaggattg cttggttcag atgttgttgt cgttagccct aaaaattcag gtgtcaaacg
    13201 tgcgcgtagc ctggctgaat atcttgatgc tcctatcgcc attatcgact accctcaaga
    13261 cgatgcaact cgtaacgaag gttatattat tggtgatgtt gaaggtaaga aagctatctt
    13321 gattgatgat attttaaata caggacgtac cttctctgaa gcttctaaaa tcgttgaacg
    13381 tgaaggagct acagaaattt atgctgtttc tagccacggt ctcttcgtcg agggagctgc
    13441 tgagcttctt gacaatacta atattaaaga aattcttgtg actgattcag tagcaacaaa
    13501 agaaaaaact cctaaaaatg tatgctatat cactgctagt gagttaattg gtgatgctat
    13561 cgtacgtatt cacgaaagaa aaccagtcag cccactcttt gcctacaata aaaagaaata
    13621 aggtgattct ttgatttatt tggacaatgc tgcaacgact cctatgtcag cagttgctat
    13681 ttcagctatg accaaggtta tgcaagaaac ccacggaaat ccttctagta ttcatggtca
    13741 tggtcgtcaa gctggcaaac tcttgcgaga agcccgtcag gaactagccc agttactaag
    13801 gacaaaacct caacatatct ttttcacttc tggtgggact gaaggcaata atactaccat
    13861 cattggctac tgtcttcgtc accaagaaca aggaaaacat atcatcacaa ctgccatcga
    13921 gcaccatgct gtccttgaaa caattgatta cttggttcaa cactttgggt ttgaagcaac
    13981 cattatccag ccagaaaatc aagaaatcac agcccagcaa attcaaaagg ctttacgtga
    14041 cgatacgatt ttggtttcta ccatgtttac caataatgag acaggaaacc tactgcccat
    14101 cgctgaaatt ggccaaatac tcaagcaaca ccctgctgcc tatcatgttg atgcagttca
    14161 ggctattggt aaaatcccaa ttcattcaga agaattgggc attgattttc tcactgcttc
    14221 tgcccacaaa ttccatggtc ctaagggaat cggttttctc tacgcatcta gcatggactt
    14281 tgattcctat ctacatggcg gagaccagga acagaaaaaa cgtgcaggaa ctgaaaatct
    14341 gcctgctatc gtaggcatgg ttgcagccct aaaagaagac ctagaaaaac aagaagaaca
    14401 ttttcaacat gtacaaaatc tagaaactgc ctttctggca gagctggagg gcattcagta
    14461 ttacttgaat agaggaaaac atcatctccc ttatgttctc aatattggat ttcctggtca
    14521 gaaaaatgac ctcttactcc ttcggctaga tttagctgga atttcaatct ctactggctc
    14581 agcctgtact gcaggcgttg tccaatccag ccatgttctt gaagccatgt atggcgcaaa
    14641 ttcagaacgc ttgaaggaat cccttcgcat cagtttgtcg ccacaaaata ccgttgaaga
    14701 cctacaaacc ctcgcaaaaa ccttaaaaga aattatcgga ggttagccat atggcatttg
    14761 aaaaaatcat tcagttaaaa aattgtcgtt acgattacac tcttagccct tctgttaaaa
    14821 aattcaccct caaagataac accttttttg aaactaaggt tggtaactat gaactgactc
    14881 gccttttgga aaaagtgcca aacagcggtg aaggcttcca actcaaaatc atcattaaca
    14941 aggaagttac aggggctaaa atcaatatca ctgataagtt tggccttcgt ctagttgata
    15001 ttttcaaatc agaagaccac catattcatc aggaaaaatt ctacttcctc atggatagct
    15061 tggtagaacg tggtgtcttt acaaaatcgg aaagatagag ccaaaatgtt tgaactgacc
    15121 tataaggact gctatcatgt agagcgtact ctcaagtatg aggatcatga ggctctcatg
    15181 ttgactttgt caggatgtgt aaccttacca gatacacttt atgtgacttc tctgactttt
    15241 aggggccaaa aagtttatca gggtctggtc ggagacctct accgttttct atctcatgca
    15301 gattttttac atcaaaacta agatttttct tagttttttc ttatttttgt tgattttttc
    15361 acaatgtttc tataaaatag aagaatagaa aggttgtgat tttgtgaaag ataaacagtt
    15421 tgctattcca aaagctacag caaaaagact ctctctctac tatcgaattt ttaagagatt
    15481 tcatgcagaa aagattgaac gtgccaactc taagcaaatt gcagaggcta tcggtattga
    15541 ttcagcgacc gtacgtcgtg atttttccta ttttggtgaa cttggtcgtc gtggttttgg
    15601 atatgatgtc aaaaaactaa tgacattttt tgccgatttg ctcaatgaca actctattac
    15661 caatgtcatg ctggttggta ttggaaatat gggccatgcc cttctccact accgcttcca
    15721 cgaacgtaac aagatgaaga ttatcatggc ctttgaccta gatgaccatc ctgaagtcgg
    15781 tacccaaact cctgacggga ttcccattta cggaatttct cagatcaagg ataaaatcaa
    15841 ggatgctgat gtgaagactg ctatcctaac tgttcccagc gtcaagtcac aagaggttgc
    15901 taatctcttg gttgatgctg gtgtgaaagg aattctcagt ttttcaccag tccatctgca
    15961 tttaccaaaa gacgtggtcg ttcagtatgt cgatttgaca agtgaactcc aaaccctcct
    16021 ctacttcatg cgaaaagagg attagaaagc gaaaatcatt ttatgaaaaa accagttatt
    16081 gggattacag gaaacgaaaa aactcatcca gatgatgaca tcatgatgag ctacgcagca
    16141 aaaggctttg ttgaaggcgt taaagatgct ggagggattc ccatcatcct accgattggt
    16201 gatcaagaaa tggcctgcca ctatatcagt ttgattgaca agctcatctt gacaggtggg
    16261 caaaatgtcg atccaaaatt ctatggcgaa cccaaaacca ttgatagcga tgactatcac
    16321 cttcaaagag atatcttcga actggccctc atcaaggaag ctattaaaca gaaaaagccc
    16381 attttctctg tctgccgtgg tacccaactc tttaacgttg ccatgggtgg aactttgtac
    16441 caagatatcg aagaccattg gcaggattct tctgttgagt acacaactca acgcttggtg
    16501 acagaaccag ataccgttct ccaagaaatc tatggagaaa tctctcatat caactccttc
    16561 caccaccaga gcatcaagga cttagcacca aatttaaaag ttgtggctca tgatcctaaa
    16621 gatggtatca ttgaggctgt catgagtacg gatgatgtcg cctttctcgg tgtccaatgg
    16681 catccagaac ttctttttga aaatcgtccc aaagataaaa aactctttga ctatgtcgtt
    16741 aatgaacttt agattaaatc tgtcttttca cgataactaa agtaattaga atgagagaca
    16801 atcaaatggt ccaagagaac aatccccatc agttcgcagg cttctttaac aagtttagtg
    16861 acatgatcat cattttggct aggcgctacc gctcctgaag gatgattgtg gaccaagata
    16921 agagaagtcg ccatatgctt gattgcatag tgcagaatct ctcgcggttc agcgatacta
    16981 cgagttacag aaccgataaa aatggtctgc tgatggatga tttgattttg agtattgaga
    17041 tagagtgcca ccaggtgctc ttgtttttta tcccctaatt cctgctgcat cttcttggcc
    17101 aacttttgac tgctgagaat actttccatt tcaagagtct cgtgtttgtg aatacgatgc
    17161 cccagttcaa tcatagcttg taattctatg gccttaacac gcccaatacc agacaaactc
    17221 tgcaattcct gcagggtcat ttttttcaaa tccgttaggc ttgaaagatt gttcaagact
    17281 ttttgggcaa tttcaaaaac gctagcttga cgtgttcctg tcctgagtaa aatagctagc
    17341 aactcttggt tactaagcgc ttcaactcct tccttggcca gcctttctct tggtaatagt
    17401 gaatcttctt ggaatgaaat actgtacata aaaatcctcc tcactttatt attcgtgaga
    17461 aggatggaaa attagatttt tttctcaatt ggagccacac tggctaaaag ttttttcaaa
    17521 cctacttctg ggaaattgat tttcaattcc tgcctagcac cgctacctga aacttccaga
    17581 acagttccct ctccccattt cttgtggaga gcaatgtcac caatggacca atttgcctcg
    17641 ctagatgctg attttgcgcc agctgtaaat tgaccaaatg gaagaccgct tgactggatt
    17701 gattttgggg cagcaccgcg tttacggtct tgaagagcct gagccaaact cataccttga
    17761 ccaaaggaaa tactaccact gctatatgat gccttaaagc ttgtatttgc aggacgagcc
    17821 agaccttgat actcaagcaa gtctgaactg atttcgttaa taaaacgagt cggacggtta
    17881 taattggtac gaccaaaaag caagcgtgag ttggcattgg tcagatagag aattttctct
    17941 gcacgcgtga tacctacata ggctagacgg cgctcttctt ctaattcatc tgtatcttca
    18001 gtcgcacgac taagtggaaa gacattttct tccatcccaa tcaaaaagac aactggaaat
    18061 tcgagacctt tggcagcatg cagggtcatc aaggtcactt ctgatgtctc ctgactacct
    18121 gaatctgtgt cggcaatcaa agccaagtca tttaagaaac gactcagttt gtccagacca
    18181 gtttcctctt ctgtcacatc agtggtgtca tcaaagttct tcgtaacaga aagaaactct
    18241 tcgatatttt caacccgtgc cttgctttct agagtcgctt gggcgttaag aatatcgaca
    18301 taacctgttt tttctaggac ggactcaacc aactctgtaa tgcttaagtg gtctagctgc
    18361 tcccgcaaat caagcatcat attggcaaaa tcccagatag attgggctgc cttacccttg
    18421 ataccagaca acataatatt agcagaagca tccagcatag acatattttg caaatttgca
    18481 aaatcacgga ttttctcaac tgtacctgga ccaattccac gtttaggctc gttgataata
    18541 cgctcaaaac taatattgtc actcaaatta gcaataaggt tgagataagc aataatatcg
    18601 cgaatttcct tacggctgta gaatttggtt ccgccaacca tggtataagg aatgttagac
    18661 ttgagcaggg cttcctcaat tgtacgggac tgggcattag tccgatagag aactgcaaaa
    18721 tccttatgaa ggaagttttg actgcgacta agttcatcga tggttctggc tacaaataca
    18781 gcctcatcca gctcatcatc ggcacgatag taaacgattt gctccccatc agcgttttga
    18841 gtccagagat ttttaggacg gcgattttta ttgtttttaa taacctcgtt ggccgcttgg
    18901 agaatggttt tggttgagcg gtaattttcc tccaacaaaa caaccttggc tttggggtaa
    18961 tccttttcaa agtccaagat attctgcata tcagcaccac gccaaccgta gatagactgg
    19021 tccgcatccc caaccacaca gatattttta aaacgggaag ccaagagttt gaccaattgg
    19081 tactgagcgt ggttggtatc ttggtactca tcaacgtgga tgtattggaa tttttgctgg
    19141 tagtaggtta aaacatcagg attttgatca aagagacgca aggtcagcat aatcaaatca
    19201 tcaaagtcaa cggattcaga ctgacgaagt tctttttgat aggctgtata acactgggcc
    19261 acaatttgcg tatacatatc gccagcttgg gcagcataag caacatcatc aatcaaatca
    19321 ttcttagcat tggaaatggt ccccaaaata gttcgttcat tccatttttt aggatccaag
    19381 ttcaactgtt tgagaatacg tttcatgagc gttcgctgtt caccaggatc cacaattgta
    19441 aaattacgat tgtagccaat atggtccgca tcgcgacgca aaatacgcac acacatggag
    19501 tggaaggtcg caatcagaca gttctgagta gctggattga ggctataagc acgctctttc
    19561 atctcacgcg cagccttgtt ggtaaaggta atggccaaga tattccaagg attgaccagc
    19621 ttttcatcaa tcaaataagc gatacggtgg gttaaaacac gagtctttcc agaaccagcc
    19681 cctgccatga ttagcaaggg accttctgtc gtttgcaccg cctcagcctg acggtcattc
    19741 attccattta ataatgcgtt catcttctct tccttactct tgaagtcaat atttctatta
    19801 tatcagaaat cagggaaaat agaaacttta cggggagtag caaaatatag tggattgaaa
    19861 ctagaatact acactgtggg tttctaaaac actcgacttt tcacccaatt ttttcataaa
    19921 ctagctcaat acctcaatag acttcctgca aaactagaat cctagttcat gattgataat
    19981 accagcaata aaattcattc gtaatccgaa gcgtttacga tgatttcgat a
//
LOCUS       DAVBNO010000035        18308 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVBNO010000035 DAVBNO010000000
VERSION     DAVBNO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18308
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.035"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            166..480
                     /locus_tag="VQ846_001385"
                     /pseudo
     CDS             166..480
                     /locus_tag="VQ846_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            657..1316
                     /locus_tag="VQ846_001386"
     CDS             657..1316
                     /locus_tag="VQ846_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET3075958.1"
                     /translation="MKNLTKIKFKENGEFNHFSGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            1341..2408
                     /locus_tag="VQ846_001387"
     CDS             1341..2408
                     /locus_tag="VQ846_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET3075959.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            2511..3521
                     /locus_tag="VQ846_001388"
     CDS             2511..3521
                     /locus_tag="VQ846_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3075960.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            3533..5224
                     /locus_tag="VQ846_001389"
     CDS             3533..5224
                     /locus_tag="VQ846_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET3075961.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            5227..5937
                     /locus_tag="VQ846_001390"
     CDS             5227..5937
                     /locus_tag="VQ846_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET3075962.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            6033..6224
                     /locus_tag="VQ846_001391"
     CDS             6033..6224
                     /locus_tag="VQ846_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075963.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKC"
     gene            6509..7510
                     /locus_tag="VQ846_001392"
     CDS             6509..7510
                     /locus_tag="VQ846_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET3075964.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            8118..8192
                     /locus_tag="VQ846_001393"
                     /pseudo
     CDS             8118..8192
                     /locus_tag="VQ846_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            8651..10012
                     /gene="trpE"
                     /locus_tag="VQ846_001394"
     CDS             8651..10012
                     /gene="trpE"
                     /locus_tag="VQ846_001394"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET3075965.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETTSVQVTKYMEVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            10009..10575
                     /locus_tag="VQ846_001395"
     CDS             10009..10575
                     /locus_tag="VQ846_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET3075966.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDDRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            10586..11590
                     /gene="trpD"
                     /locus_tag="VQ846_001396"
     CDS             10586..11590
                     /gene="trpD"
                     /locus_tag="VQ846_001396"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET3075967.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIARVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            11587..12354
                     /gene="trpC"
                     /locus_tag="VQ846_001397"
     CDS             11587..12354
                     /gene="trpC"
                     /locus_tag="VQ846_001397"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET3075968.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            12341..12940
                     /locus_tag="VQ846_001398"
     CDS             12341..12940
                     /locus_tag="VQ846_001398"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET3075969.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAV
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            12918..14141
                     /gene="trpB"
                     /locus_tag="VQ846_001399"
     CDS             12918..14141
                     /gene="trpB"
                     /locus_tag="VQ846_001399"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET3075970.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDMTGQDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            14134..14910
                     /locus_tag="VQ846_001400"
     CDS             14134..14910
                     /locus_tag="VQ846_001400"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET3075971.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(14882..14983)
                     /locus_tag="VQ846_001401"
                     /pseudo
     CDS             complement(14882..14983)
                     /locus_tag="VQ846_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            15212..15352
                     /locus_tag="VQ846_001402"
     CDS             15212..15352
                     /locus_tag="VQ846_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075972.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WT"
     gene            15389..15943
                     /locus_tag="VQ846_001403"
     CDS             15389..15943
                     /locus_tag="VQ846_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3075973.1"
                     /translation="MFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFPL
                     VQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNSN
                     IEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDIP
                     NFSELSETEDFEIIPRIEKQEGQK"
     gene            15958..17109
                     /locus_tag="VQ846_001404"
     CDS             15958..17109
                     /locus_tag="VQ846_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET3075974.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(17161..17820)
                     /locus_tag="VQ846_001405"
     CDS             complement(17161..17820)
                     /locus_tag="VQ846_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET3075975.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLSIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSITELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            17890..>18308
                     /locus_tag="VQ846_001406"
     CDS             17890..>18308
                     /locus_tag="VQ846_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3075976.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGSRIPFLLTLQ
                     ALEEVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQGQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVR"
BASE COUNT         5903 a         2959 c         3937 g         5509 t
ORIGIN      
        1 tactatatta tcgcttacca atatttgctt ttatatttgc taaactttca gaaaaaaagt
       61 tggagtatat gatgctaggt gactgcgtca tgttaataaa cgaaatggaa ataactgatc
      121 atagagtaga taacttattt gaaaaaggga aaaatgagat aaaagatcca tgtagtagat
      181 ttagcaatag gacatataaa agctttagaa aaagtatctg aaaaaacaga tgtttatatc
      241 tataacctag gctcgggaga aggcactagt gtattacaac ttgtaaatac atttgaaagt
      301 gttaataaga tccctattcc ttataaaata gttccaagac gttcaggaga cgttgcaatt
      361 tgttatgcaa atgcagacaa agcatataag gaattaaatt ggaggacaac aaaatcgatt
      421 gaagacatgt gtagagatac atggaattgg caatcaaaaa atcccaatga ctataattga
      481 tgatactgtc tatatgagtg ctgtattttg gaaaaattac ttaacctttt aatgaaatgc
      541 atatttaatg aaagataaat aaaaaaagtt tgacagcgct tgacaaagga aacggtttca
      601 ttgtataatg taaacatggt gacaccgatg tcaaatataa aaaagaaggt ctttatatga
      661 aaaacttaac aaaaattaag ttcaaagaga atggggaatt taatcatttc tctgggaata
      721 cagttgtagc aaatctttat actaaacaag atttgatgga agttgttgat attattcaat
      781 cacgttatag agaattacca tttatcgata agtttacttt aactccaagg aattctattc
      841 acatgactgt aattgaattg ttgtgccatg aaaatcgtga aacggagttt tggagcagta
      901 atcttcctct agatacacct ttacaggaaa tacatgatta ctttgctaaa caacttgaaa
      961 tttttccatt gttggatgaa gaaattcata tgcgtataac tgaaatggga aaacaaaaca
     1021 tactagttga acctgcagat gaagcttctg caaagagatt agaagaaatt cgtacttatg
     1081 tctcagaaaa agcaggtgtt tgtttcccta atcatgatag atatcaattc catatttcaa
     1141 ttgggtatct tcggattcct ctaaccgaag aggaggaaga agagtttact aaagtcagag
     1201 cagaattaac tgaaatttta ttagagaaga ttccaactat tactgtaaac cgtattgact
     1261 atactgtatt tgaagatatg agacaatttg ttccatatca cgaaaaattt aaataaaata
     1321 ataaaaaagg agaacttatc atgaaaaaaa tgaaagtttg gtctactgta cttgcaacgg
     1381 gagttgctct tactacactt gctgcttgct ctggaggttc aaattctacg actgcttctt
     1441 catctgaaga aaaagctgat aaaagtcaag aattagtaat ctattcgaac tcagtctcaa
     1501 atggtcgtgg tgattggtta actgctaaag caaaagaagc tggttttaat ataaaaatgg
     1561 ttgatatcgc tggcgctcaa ttagcagacc gtgttattgc tgagaagaat aatgcagttg
     1621 cagatatggt atttggaatt ggtgctgttg actcaaataa aattagagat caaaaattac
     1681 tagtacagta caagcctaaa tggttagata aaattgatca atctttatca gataaagata
     1741 attattataa tcctgtgatt gttcaaccat tagttttaat tggggcgcct gatgtaaaag
     1801 aaatgcctaa agattggact gaattaggta gtaagtataa aggtaaatat tcaatttatg
     1861 gtcttcaagg tggtacagga cgggcaattc tagcaagtat cttagttcgt taccttgatg
     1921 aaaaaggaga tttaggcgta tcagaaaaag gatgggaagt tgcaaaagaa tactttgcaa
     1981 atgcctatac tcttcaaaaa ggtgaaagct cagttgttaa agcgttagat aaagaaagcc
     2041 caattcaata cggtatgatc tggggttctg gagcgttaat cggacaaaaa gaacaaaatg
     2101 ttgaattcaa agttatgact cctgagattg gtgtaccatt tgtaactgaa caaactatgg
     2161 ttttaagcac tagtaaaaaa caagcgttag ctaaagaatt tattgattgg tttggtcaat
     2221 cagaaattca agtagaatat agtaaaaaat ttggctctat ccctgccaat aaagatgccc
     2281 taaaagagtt acctgaagat acgaaaaaat ttgttgatca agtgaaacca caaaatattg
     2341 actgggaagc tgttggaaag catttggatg aatgggtaga aaaagctgaa ttagaatacg
     2401 tacaataaga aataagagtt attttttatg tccaagtctc tctattattt ctaaatttat
     2461 ggaagaaata aagagaggct tttggtttag taatagaggg agttagtgag atgattaaat
     2521 ttgataatat tcaaattaaa tatggtgatt ttgttgcaat tgataatctg aatttagata
     2581 tacatgaagg ggaattcttt acatttcttg ggccttcagg atgtggtaaa tcaactactt
     2641 tgagagcatt ggtaggtttt ttagatccat catcaggaag tattgaagtt aatggaacag
     2701 atgtcactca tttggaacct gaaaagcgtg gaattggtat tgtatttcaa tcttatgcgc
     2761 tatttccaac tatgactgtt tttgataata ttgcatttgg tttaaaagtt aagaaggtag
     2821 ctccagatgt tattaaagct aaagtatcag cagtggcagc aaaaattaag atctctgatc
     2881 aacagttaca gcgtaatgta tcagaattat ctgggggtca acaacaaagg gtagcattgg
     2941 ctcgtgctct ggttcttgaa cctaaaattc tttgtctaga tgaaccattg tcaaaccttg
     3001 acgcaaaatt acgtgtagat ttgagaaaag agttgaaaag acttcaaaaa gagttaggta
     3061 ttactacttt atatgttact catgatcaag aggaagcctt gactttatct gatagaattg
     3121 cagtctttaa caatggatac atcgaacagg tcggtacacc agtagagatt tatcataatt
     3181 ctcaaactga atttgtatgt gattttattg gagatattaa tgttttgacc gatgaaacag
     3241 tccacgaagt attactgaaa aatacaagcg ttttcttaga agataaaaaa ggatacattc
     3301 gattagagaa agttcgattc aatcgtgaaa ctgaacaaga tttcattcta aaagggacaa
     3361 ttattgatgt tgagttttct ggagttacaa ttcactatac aataaaagtt tctgaaagtc
     3421 agattcttaa tgtaacaagt attgatagtc aggctgctat tagatctgtc ggagaaagtg
     3481 tggaattatt tatcacacca tcagacgttc tgcaatttta aggaggtgca gtatgcgtca
     3541 taaattaaat ttaaaagatt ggcttattcg tttagggtta atctggttct tagtaacatt
     3601 tattatttat ccaaactttg atctagtagt gaatgtattt gtaaaaggag gagaattttc
     3661 ccttgatgct gtacatcgtg ttctaaaatc tcagagggca cttcagagta ttatgaacag
     3721 ttttaagtta gcattttcac tcattattac agttaatgtc gtaggtattc tttgtgttct
     3781 atttacagag tactttgata ttaaaggtgc taaaatttta aaattaggtt atatgacctc
     3841 tttaatttat ggaggagtgg ttttagcgac tggatataaa tttgtctatg gtccttatgg
     3901 attgattaca aaatttttac aaaatgttat cccttcttta gactctaact ggtttattgg
     3961 gtatggtgca gtcttattca ttatgacatt ttcaggaact gctaatcata cattgttttt
     4021 aacaaataca attcgaagcg ttgactatca cactattgag gctgctcgaa atatgggagc
     4081 aaaaccattt actgttttcc gaaaagtagt gttaccaacc ttaattccaa ctctatttgc
     4141 acttactatt atggtttttc ttagtggttt atctgcagta gcagcaccca tgattgttgg
     4201 tggtaaagaa tttcaaacta taaatccaat gattattaca tttgcaggga tggggaattc
     4261 tcgtgattta gctgccttac ttgcaattat tttaggtatt gcaactacaa ttttgcttac
     4321 tatcatgaat aagatagaaa aaggtggaaa ttatatttct atctctaaga ctaaagcgcc
     4381 tcttaaaaaa caaaaaattg cgtctaagcc ttggaatatc attgctcaca ttgtagcata
     4441 tggattgttc acagttttca tgcttccact aatttttata gtattatact catttacaga
     4501 tccagttgca attcaaacag gtaacttaac attatcaaac tttactttag aaaattatcg
     4561 cttattcttt agtaatagtg cggcattctc tccattcttg gtcagcttta tttattctat
     4621 tattgctgcg acaacagcaa caattctcgc agttgtattt gctcgtgttg tcagaaaaca
     4681 taaatctcgt tttgatttct tatttgaata tggtgctcta cttccttggt tactaccaag
     4741 tacactttta gcagtaagtt tattatttac ttttaatcag ccacaatttc ttgtcttgaa
     4801 tcagattttg gtaggtagtt tggtaattct acttattgca tatatagttg taaaaatccc
     4861 attttcttat agaatggtac gtgctatttt atttagtgtt gatgatgaga tggaagatgc
     4921 agcaagaagt atgggtgctt caccttttta tactatgatg aaggttatca ttccatttat
     4981 tttaccggtt gttctctctg ttattgcttt aaactttaac tctttattaa ctgacttcga
     5041 cttatctgta ttcctttacc atcccctagc tcaaccatta ggtattacga ttcgatctgc
     5101 aggtgatgaa acagcaacat ctaatgcaca agctctggta tttgtttata caattgttct
     5161 gatgattatt tctggaacgg tattatactt cacacaaaga ccggggcgta aagtaaggaa
     5221 ataatcatga cagccactag tcttgggtta tcaaatattg aaatagttgt caggattgtt
     5281 ttatcagtag tcattggtag tataattggt ttagagagag ggagcaaatc ccagcctgca
     5341 ggcatccgaa cttatagtat tgtttgtcta gctgcatgtt tgattatgat gacgaatgaa
     5401 tacgtatctt ataaatttgg gacaggagat cctacacgat taggagctca agttatatca
     5461 ggtgtgggtt ttctaggcgc tggaacgatt cttattacag ataaaaagaa aattacaggt
     5521 ctgacaactg cagcaggcat ttgggcttcg gcaggaattg gattagctat tggagtaggt
     5581 ttttatgagg gagctctttt agtagctatt tctgtttggg gtgtgatatc catgttccaa
     5641 ccactaaaaa aatatctgca aaatcgttct aaaatgattg aattgtatat agtggttaaa
     5701 tctacagaag cctataatcg tgtattggtt tattgtgcag aaaatggtat cagaatgacc
     5761 gattcaagaa cagcgtttgg agatgttaat tctgacagaa ttgaatactt tgatgttcca
     5821 gataaaaaaa tagcgtcatt tattactctg gaattatcag gtagatttga acatcttcga
     5881 ctgatggaag aaattgctaa tattgtaggt gttatttatg tcgaagaaat tagctgaaat
     5941 ttagtaaaca aagggacaga tgtttttgtc cttttgtttt ttagatgttt taatcatcta
     6001 atttttgagt agatttttgc tcagaggttt ttatgaaaga ttataaaatc aattttgatt
     6061 taggtaaaat agaatatttt gataataatt gtttgataca agtatacaaa tttatttctt
     6121 tttatgatat ttgtgaaatg gtgtttgcct ttcatttacc tcctgatgag ttgataacaa
     6181 atgttatctt taaagaaaaa attaactcta tgttaaaatg ttaaattgac aggctattat
     6241 atgtttttat aaatcctaca cattttactg aaaaagttaa tctacaattt tatggtagtt
     6301 ttttctcata tgaatttatt tgtcgtgaag ttggaaacat tcttaaaaat aaaggtgtga
     6361 aatgcaattt aaactttttt gaagggaaag aatatttgta aaattaattt tgtgaatttg
     6421 tggatcttaa ataatttact aaattctctt gttaaaaaaa attaaatatt gtatagtaat
     6481 attagaatgt tcgtaatgga ggactgaaat gaaaaatata aatgggaaaa aggtaactat
     6541 ttatgatatt gcacgtttat caggtttttc tccaaaaaca gtttcacgag ttattaatgg
     6601 tggagtaaat gttaaagaag aaacttatca agcgattcaa aaagttatag aggaattgtc
     6661 gtatattcca aatgcgtatg caaaaaattt aactaaaaaa gaagctataa atatattgat
     6721 ttctgtaaaa aaaatcgact cttttccttt aatttggttt catacgctgt tagataaagt
     6781 gttacggacg tgtaaagaat ttggtgttaa tgctatagtt gagtactttg gagaggagga
     6841 tacaattagt aattcaatta tatcaagcac aggtagccta gtagatggtg ttattgtttt
     6901 ttatgaaagt gtagatgata tcagaattca gtatttaaag aagaaccata tgccttttct
     6961 tgttttcggt gaatctcaaa cgtctggagt agtctatgta tctaataata attttcaagc
     7021 tacttatgat atgatgaaag ctgtaactga agaaaaattt aaaaatatgt ggttgcttat
     7081 gggaggagaa tcccatgtta ataaggatcg tgaaagaggt gtgcgttctt ttctgaatga
     7141 taaaaattac tttatggatt taaaagtcat ttatggctta tctacaattg attcagttta
     7201 ttcttatgca atgcagcact tgactaccca aaattatccg gacattattt ttgtttcagg
     7261 agatgaaaag gttcaaggct tgattcgtgc gtgctatgag aaaggaattc ttattccaga
     7321 tgatatttct atcattggat ttgataatat tcctatttct cagtattata ctccagcttt
     7381 gtctacaatt gctcccaatt atgttaaatt ggctaaagaa atgatagaag gtgttttagc
     7441 aattattaaa ggggaaagtg tcacatctgt tgaagtttct cctaaatttg ttaggagaca
     7501 gagtttctag tattgtttaa ttcttgaaaa tcctagtgga aaagatggag tgttctaaaa
     7561 taagttctgt ttagcctcca ttcagtttct attgagttgg atccaatttc taacgagtgg
     7621 gacatgttca tgttcgatct aaggaagaca tggtcacatt cgaatgatat tttcaaaaaa
     7681 atcgtaaaaa agtcttgaca aagaaaaaac aaagtattaa aataagttca acaaatcaga
     7741 aaagtaacta tttttgatct tcagggagcc tgtggtgatt gtgaataggt ggttggaagt
     7801 agtgaaagtg ggctgatttt gaaaatgaat ttgaaacaat gaaaattcgg tgcgcacacc
     7861 ttacagtgca gcttgttgtt aaacaaggca gagatgtaaa gggatagtcc ctttataatt
     7921 gaggtggcac cgcgttacca acgccctcac acggaagtat attctgtgtg tgggcttttt
     7981 tctatccgtc gtttgcttta tcttttatta gggccttaac tcgctttgat gaacttgagt
     8041 tctatctaca gctccttgcc tcctactaaa agtaaacaaa aaggctggac taatacgaaa
     8101 aaagctagta atactcaatg aaaatcaaag agcaaactag gaaactagcc gcaggttgct
     8161 caaaacactg ttttgaggtt gcagatagaa ctgacgaagt cagtaaccat acctacgata
     8221 aggcgacgtt gacgcggttt gaagagattt tcgaagagta taaaggtgta ccgagatgaa
     8281 catctcttca tcaatgtctc tggtcgtgga ggcaaggacc tagctgcgat tgcagattac
     8341 tagaagctaa aaaataatag atggaaaaat tacagtcttt tctctcacag agagcttgtg
     8401 gttgctgaaa acaagcagag aaagctgtaa agttgggttc atctgaaacg agagaagaaa
     8461 acatcattct caagggagtg ccctttatcg cacagcctgt tacaggaggt atctgagaca
     8521 ggaatgagag ataggagaaa tcctataatt gaggtggcac cgcgaatttc gtcctcacgc
     8581 aagttatttt gcgtggggat ttttcataca gtcgccacga actagaatta gaactgaaag
     8641 ggaaattaca atggaacgaa tcattcatgg agatgtctta tcaccaatct tggcttatat
     8701 gcgcttaaag gggcaacata aggttatttt agagagtatt ccgagagaca aggaaacggc
     8761 tcgtttttct atcctagcct ataatccagt ttttgagatt aagtttgaaa atggagttct
     8821 ctatcaaaat ggtcaagtga ttgatcggga tcccttggat ttcctttatg aagtgattca
     8881 taagagtcag caccattcag agctcccttt tggtggtgga gccattggtt ttgtgggtta
     8941 cgatatgatt tcgctttatg aagaaattgg tcaaatccct gaggatacaa ttgggacgcc
     9001 agacatgcat ttctttgtct atgagagcta tatggtcttt gaccacaaga aggagaagat
     9061 acatgtcata gaggatgcac tttatagtga gcgcagtcaa gaggccttgg aaaaatcctt
     9121 gaaccaagtg cttgaggaat tacgcattcc tgctccaaat gaatttgaag acttggattt
     9181 atcaccactt gacttcaaac cgcatatcgc tccacataag tttgagggaa tggtagaaac
     9241 agctcgtgac ttgattcgta acggtgatat gttccaatgc gtactcagcc agcgcttctc
     9301 agcagaggtt actggaaatc catttgactt ctacagaaat ctccgcgtga ctaatccttc
     9361 taattacctt tatttctatg attttgggga ttatcaaatc atcggagcca gtccagaaag
     9421 tttggtttct gtcaaaaatg gcatcgtgac aaccaatccg attgcaggga cgcgaccaag
     9481 aggggctacg gatgaagaag acaaggcctt ggcgactgac ctcctctctg atgagaagga
     9541 aacagcagag catcgaatgt tagtagacct gggtcgtaat gatattggtc gcatctctga
     9601 aacgaccagt gtccaagtca ctaagtatat ggaagtggag ctcttccgtt atgttatgca
     9661 tctgaccagc gtggtcaagg ggcgtttgct tccagaactc actgccatgg atgccttgaa
     9721 agctacactt cctgctggaa ccgtttctgg agcaccaaag attcgtgcta tgagacgtat
     9781 ctatgaactg gaaacagaaa aacggggcgt atacgcagga gcaatcggct acttgtctgc
     9841 gacgggtgat atggatttgg ccattgccat ccgaaccatg attctcaaaa atcaaagagc
     9901 ctatgtgcag gctggggcag ggattgtcta cgactctatc gcccaaaacg aataccaaga
     9961 aaccattaac aaagcaaaat ctatgactag aattggagaa ctaagaccat gattttattg
    10021 attgacaact atgattcttt tacctataac ttggcccaat acattgggaa ttttgcagaa
    10081 gttcaggttc tgagaaatga tgattccaag ctgtatgaag aagctgaaaa agcagatggt
    10141 ctggtctttt ctcctggtcc tggttggcca gttgatgctg gaaagatgga agacatgatt
    10201 cgtgactttg caggcaagaa gccgattcta gggatttgtt taggccacca agccattgca
    10261 gaagtctttg gtggtaagct aggtttggct ccaaaagtca tgcatgggaa acagagcaat
    10321 atcaactttg aagcgccatc tgttttgtat caaggtattg aggatgaccg tgcggtcatg
    10381 cgttatcaca gtattttgat tgaggaaatg ccagaagact ttgaagtgac agctcgttcg
    10441 actgatgacc aagccatcat ggggattcaa cataaaaacc taccgattta tggcttccag
    10501 taccatccag agagcattgg aacgccagac ggcttgtctt ctattcggaa ttttatcgag
    10561 aaggttgtaa agtgaggaaa ctaggatgaa agagattatt gaaaaactag caaaatttga
    10621 aaatttatca ggtgtggaaa tgacggatgt cattgagcgt atcgtaactg ggcgtgtaac
    10681 tgaagcgcag attgcttctc tcctcttagc tcttaagatg aagggggaaa cacctgaaga
    10741 acgcacagcc attgcccgag tcatgagagg acatgcccag catattccaa ctgagattca
    10801 tgatgctatg gacaactgtg gtacaggtgg ggacaagtct ttcagtttta atatttccac
    10861 aactgcagcc tttgtcttgg ctggtggcgg tattcacatg gccaagcatg gtaaccgctc
    10921 gatttcttct aaatctggtt ccgcagatgt cctcgaagcc ttgggaatca atcttgacct
    10981 caaaccagct gaactaggta aggtctttga taaaactggc atcgtctttc tcttcgctaa
    11041 aaatatgcac ccagctatga aatacatcat gccagctcgt ttggaattgg gaattccaac
    11101 gattatgaac ttgactggtc cactgattca tccaatggct ttagaaacac agcttcttgg
    11161 aattagtcgt ccagaactcc tagaaagtac agctcaggtt ttgaaaaata tgggtcgcaa
    11221 acgtgccatc gtggttgctg gaccagaagg gttggatgaa gctggcttga acggaacaac
    11281 caagattgca cttcttgaaa atggcgaaat cagcttgtca agctttactc cagaggattt
    11341 gggaatggaa ggctatgcta tggaagatat tcgtggtggg aatgctcagg aaaatgcaga
    11401 aattttgctt agcgttctga aaaacgaagc aagtccattc ttggaaacga cagtcttgaa
    11461 tgctggtctt ggtttctatg ctaatggtaa gattgatagc atcaaggaag gagttgcctt
    11521 ggcccgtcaa gtgattgcta gaggcaaggc ccttgaaaaa ctcagactgt tacaggagta
    11581 ccaaaaatga gtcaggaatt tttagcacga atcttggagc agaaggcgcg tgaggtggag
    11641 cagatgaagc tggagcaaat ccagcctctg cgccagacct atcgcttggc agaatttttg
    11701 aagaatcatc aggaccgctt gcaggtaatc gctgaggtca agaaagctag ccctagtttg
    11761 ggagatatca atctcgatgt ggatattgtg caacaggccc agacttatga agaaaacgga
    11821 gcagtgatga tttcggtgtt gacagatgag gttttcttta aagggcattt ggattatcta
    11881 cgggaaattt ccagtcaggt agagattccg acgctcaaca aagactttat catagatgaa
    11941 aagcaaatca tccgcgctcg caatgcaggt gcgacagtta tcttgcttat tgtggcagcc
    12001 ttgtccgaag aacgcctcaa ggaactgtat gactacgcga cagagcttgg tctggaagtc
    12061 ttagtggaga ctcacaatct agctgaacta gaggtagccc acagacttgg tgctgagatt
    12121 atcggggtca acaaccgcaa cttgactacc tttgaggtcg acttgcagac cagtgtagat
    12181 ttagcccctt actttgagga aggtcgctat tacatttctg aatctgccat tttcacaggg
    12241 caggatgcgg aacgactagc cccatacttt aacggaattt tggtagggac agctcttatg
    12301 caggcagaaa atgtggccca gagaatcaag gagttgcaga ttgacaaagg ttaaaatttg
    12361 tggactatcg accaaagaag cggtggaaac agccgtttca gcaggagccg actatatcgg
    12421 ttttgtcttt gcacctagta aaagacaggt gactttagaa gaggcagctg tgttggcaaa
    12481 gcttattcct gcagatgtga aaaaggttgg agtatttgtt tcaccaagtc gggtagaact
    12541 gctggaagcg attgacaaag ttggcttgga cttggttcaa gttcacggtc aggtggcaga
    12601 tgatttattt gagaatttgc cttgtgccag tattcaggct gtgcaggtag atggaaatgg
    12661 gcatgtcccc aattctcagg cagattatct actctttgat gcccctgtgg caggaagtgg
    12721 ccagcccttt gattggggtc aactggatac gactggacta gcacagccct tctttatcgc
    12781 aggtggcctt aatgaagata atgtagtaaa agcaattcaa cattttactc cctatgcagt
    12841 agatgtatcg agcggagtgg agacagatgg acaaaaagat catgaaaaga ttagaagatt
    12901 tatagagagg gtaaagcatg gcatatcagg aaccaaataa agatggattt tacggaaaat
    12961 tcggcggacg ttttgtccca gaaacattga tgacagcagt tttggagttg gagaaggcct
    13021 accgtgaaag tcaggcagac ccaagtttcc aagaggaatt aaaccagctt ttgcgccagt
    13081 acgtaggacg tgaaactcct ctttattacg caaaaaactt gacccagcat atcggcggag
    13141 ccaagattta tctcaaacgg gaagacctta accatacagg agcccacaag attaacaatg
    13201 ccttaggaca agtttggctt gccaaacgca tgggtaaaaa gaaaattatc gcagaaacgg
    13261 gtgctggtca gcacggtgtg gcaactgcaa ctgctgcggc cctctttaac atggaatgta
    13321 ccatctacat gggtgaggaa gatgtcaaac gccaagccct caatgtcttc cgtatggagc
    13381 ttttgggagc taaggtcgag gccgtgacag atggttcgcg cgtgctcaag gatgcggtca
    13441 atgcagccct tcgttcatgg gtagctaata tcgatgatac ccactatatc cttggttctg
    13501 ccttggggcc tcatccattt ccagaaatcg ttcgtgactt ccaaagtgtc atcggtcgag
    13561 aggctaaaca acagtaccgc gatatgacag gtcaagatct gccagatgcc ctagtagcct
    13621 gtgttggtgg tgggtcgaat gctatcgggc tcttccatcc ctttgtagaa gatgagtcag
    13681 tagctatgta tggaactgaa gcagcagggc ttggtgtgga tacagagcac cacgctgcta
    13741 ccttgaccaa gggtcgtccg ggtgttcttc acggttctct catggatgtg ctccaagatg
    13801 cccatggtca aattcttgaa gccttctcta tctcagcagg tttggactat cctggtattg
    13861 gtccagaaca ttctcactac cacgatatca aacgtgccag ctatgtccct gtgacagatg
    13921 aagaagcctt ggaaggattc caactcttgt ctcgtgtgga agggattatc ccagccttgg
    13981 aatctagcca tgctatcgct tttgcggtga aattggccaa agaacttgga ccagaaaagt
    14041 ctatgattgt ctgcctatca ggtcgtgggg acaaggatgt ggttcaagtc aaagaccgct
    14101 tggaagcaga tgcagcaaag aagggagaag ctcatgccta agacactaac agaaaaattg
    14161 aatgctatta aagcagctgg aaaaggaatt tttgttccct atatcatggc tggtgaccac
    14221 gagaaaggtt tggacggtct tgctgaaaca attcactttt tagaagattt gggtgtctct
    14281 gcaattgaag tgggtattcc cttttcagac cctgttgcag atggacctgt tatcgaagaa
    14341 gctggcttgc gcagtttagc ccacgggacc tctacccagg ctttagttga aaccttgaaa
    14401 accattgaaa cagagattcc actggtcatc atgacctact tcaaccccct ctttcagtac
    14461 ggtgtggaga actttgtcaa agatctggct gatacagctg tgaagggctt gattatccca
    14521 gacttgcctc atgaacatgc caactttgta gaaccatttt tggcagatac agatatcgcc
    14581 ttgattcctt tagtaagctt gaccacagga attgagcgcc agaaagagtt gattgaaggc
    14641 gcggagggat tcgtctatgc cgttgccatc aatggggtga cagggaaatc aggcaattac
    14701 cgtgcagatt tggacaagca cttggcgcaa cttcatcaag tggccgacat cccagtcttg
    14761 acaggttttg gtgtatctag tcaagccgat ctggaacgct tcaatgcggt gtcagatggt
    14821 gttatcgtcg gttcgaaaat agtaaaagct ctccaccaag gagagccgat tcaggatttt
    14881 atcagacaag cagtagctta ccaaaaataa tcaaacaagt agcaagtaag aggaaaggca
    14941 cgaaaggaag tctttccttt ttcttttgca ggcgaaaggc tagttttata gtgaattgaa
    15001 tctggaatat tacactacgg ttcctaaaat attttaagaa agtgatttga atttcccaat
    15061 aaatttgttc agtttttaat tgattttact atagaagata taattttcta aattactctg
    15121 acacagaact ttaagaaaaa gctaattatt gtagtaattt ctttaagaaa atgatataat
    15181 tcattaaaaa gtatttggag gaggaagaat tatgttgaat caggatctct ttgattcgct
    15241 tgaggcacaa aaaattgtag atactttgat gaaagggcaa aaagattatg tagatgaacg
    15301 tctagaaaaa agagagacaa tgatagtgtc gaatggttat gcatggacgt gacctaatca
    15361 tattgatact gcatttgcat cagcagattt gtttgagtat aaattacaat tagcaggaca
    15421 gacttgggga tatttagaat ttgaaacaaa tacagaaaaa tatgggaaag tattgttaat
    15481 tataaagggt aagaagcgac ttacgaacca atttcctttg gtacaaaaaa ataagagtgg
    15541 ctacttattt gaatatgctc agatgaatac actttatctt aatcaacatt cttcctacaa
    15601 aaatgatgaa gatagtcatt cctttccaat tcagatggag ttagtttctg atgaaatgat
    15661 tcaagaaatt gaacaagcta ctaaaaattc gaatatcgaa aaatttatga ttttaactta
    15721 tgaggcggac tcagaaaaca atattatatc tgtagatgtt gttatgcctg atgcacgaac
    15781 tggtcagtta cacttgattc aagatttgtc tgagtatatt caatcaagtt cgtaccattt
    15841 cgaagaagct aaatatcaag atattcctaa tttttcagaa ttatcggaaa cagaagattt
    15901 tgaaattatt ccaagaatag aaaaacaaga aggtcaaaag tagtaaggag tatgtaaatg
    15961 tttaatggtc gggtattgaa agaattacgg ctgttaaatg gtttaagtag agcagaatta
    16021 gctcagagaa ttaatttaac ggaacaagcc atttggcagt ttgagtccaa cgaaacgaaa
    16081 cctaaattat caaccaaaat gcatttggcc aaccaatttc atgttgattt aacttatttt
    16141 gaacaggaag aagagagcat tcgatttgat tcttctgtaa ttgcctttag aaatgcagac
    16201 ctagcaacac ggaaaacaat agatattcaa actatgtatt tacataaggt agatagtttg
    16261 attgattatt ttgaaagttt tgtaattata cctaatatta taattcatga cctaagtaat
    16321 gtagtgagtg aatcttatca taagggagaa tccattgagg aattggctct ttatgccagg
    16381 gaaaaattag gtatttcaaa agataatcat gatttgcttt ataaattaga acgttcaggc
    16441 atctatatcg tggaacgatt aattaatggc caagctgatg cttatagcgc atggtcaaaa
    16501 ttgggaagac cttatattgt gttgggaacg aataaatcat ctgtacgtcg aaattttgac
    16561 ttagctcatg agctaggaca tattctttta cataaatata aagatatgaa tgaagatggc
    16621 gatcgtttgg agcaagaagc aaattatttt gcatcatgtt ttttattgcc aaaagaagag
    16681 tttttagtca aatttgaaga gagggttggc aagcgtgtca gcaatcctga tagttatatt
    16741 ttattgaagt cggatttgaa tgtttcgata caggctttag agtatcgagc ttttaagtta
    16801 ggattattga ctccaaagca acattcttac ttttatcgtc aaattgcgca aaaaggttac
    16861 aaaatgattg aacctttgga tgatcaaatt tttgttaaaa aaccaagcaa agtaaagagt
    16921 attctggacg tcgttttgag taatcatcta gtcagtctag cgactataat gtctaaacaa
    16981 agtattcgtt tacagtttat aagcgaaata ttttcagtcg aaatgaaatt ttttgatcag
    17041 tatcaagaag atagaagaac agatcgattt gataacatca tccctttgta caaaagaaat
    17101 aatttataat ttaactgtga aataatttca tgaaggacat atggcagaaa ttggacttta
    17161 tcaaacaagc agtagcttac caaaaataat caaacaagta gcaagtaaga ggaaaggcac
    17221 gaaaggaagt ctttcctttt tcttttgcag gagaaaggct aggatacccg tcgcagaagc
    17281 aaactgaatc aagatcagta actccgttat gctaaagacg agagcacaag aagctaaaaa
    17341 gaggaaatcc cctgcgccca tgcggatatc gataaaatga gccaaaattc caagagcaag
    17401 gaaggagacc atgaccagat tccagccaga ggaagctatt aggattaggt ggaaagtcat
    17461 ccagaccagt aagggatatt cctgatggcg aaagtcatag atgctcaagg tcaaaccagc
    17521 ggtgattagg acgacttgcc ccaaggaaag caattcccaa gagtaaagca gaaagaggag
    17581 tcctaagcct agttcaaaga gggcatacca gacaggatag cggaccttgc agtagcgaca
    17641 gcgaaagcga ttgaagacct gtgagagaat cggaatcaaa tctaagggac gcaagggagt
    17701 ctgacaggaa tcgcagtgac tggctgaact gataatggat tgctctggaa aacggtcaat
    17761 gaccaaacca agaaaggaag cgagaatgct cccgacgaga aaaaaataaa aatcaatcat
    17821 acttatctat tcgtaaaaaa tgggagaaat agtataatgg agaaacatag ataagatggt
    17881 gaggaaaaga tgacaattcg ttttgaagaa aaggtgagta tagaaaacgc tcaatttgta
    17941 tgccaatggt ccaactccct aggcaaatcc tttcaagaac aatggatggg atcaaggatt
    18001 ccttttctac tgacccttca agccttggaa gaagtctttt caatctttga tgaacaagag
    18061 tttgtgggac ttatccagaa aatcaggcta gaagacagca atcttcatat cgggagattt
    18121 tttatcaacc cccagaaaca ggggcaaggc ttaggtagcc aggctttaag gaaatttgtt
    18181 agtttggcct ttgaaaatga agatatagat agtatttctc taaatgtctt cgaggcaaat
    18241 caaagagctc agaatcttta ccaaaaagaa ggatttgaaa tcgttcaaat ggttgaagca
    18301 cctgtacg
//
LOCUS       DAVBNO010000036        18177 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVBNO010000036 DAVBNO010000000
VERSION     DAVBNO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18177
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.036"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(62..859)
                     /gene="proC"
                     /locus_tag="VQ846_001407"
     CDS             complement(62..859)
                     /gene="proC"
                     /locus_tag="VQ846_001407"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET3075977.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMDAVHQAYKRTQELGK"
     gene            complement(863..2125)
                     /locus_tag="VQ846_001408"
     CDS             complement(863..2125)
                     /locus_tag="VQ846_001408"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET3075978.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVS
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(2135..3265)
                     /locus_tag="VQ846_001409"
     CDS             complement(2135..3265)
                     /locus_tag="VQ846_001409"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET3075979.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(3345..5228)
                     /gene="cbpE"
                     /locus_tag="VQ846_001410"
                     /gene_synonym="pce"
     CDS             complement(3345..5228)
                     /gene="cbpE"
                     /locus_tag="VQ846_001410"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET3075980.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTGGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(5231..6118)
                     /locus_tag="VQ846_001411"
     CDS             complement(5231..6118)
                     /locus_tag="VQ846_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET3075981.1"
                     /translation="MEIKIETGGLRLDKALSDLTELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVIAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIDHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(6108..6569)
                     /locus_tag="VQ846_001412"
     CDS             complement(6108..6569)
                     /locus_tag="VQ846_001412"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET3075982.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRASQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(6566..7474)
                     /locus_tag="VQ846_001413"
     CDS             complement(6566..7474)
                     /locus_tag="VQ846_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET3075983.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            7467..7664
                     /locus_tag="VQ846_001414"
     CDS             7467..7664
                     /locus_tag="VQ846_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3075984.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(7716..8387)
                     /locus_tag="VQ846_001415"
     CDS             complement(7716..8387)
                     /locus_tag="VQ846_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET3075985.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(8441..8662)
                     /locus_tag="VQ846_001416"
     CDS             complement(8441..8662)
                     /locus_tag="VQ846_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET3075986.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            8934..9743
                     /locus_tag="VQ846_001417"
     CDS             8934..9743
                     /locus_tag="VQ846_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET3075987.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHIAAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKLRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(9924..10799)
                     /gene="aguB"
                     /locus_tag="VQ846_001418"
     CDS             complement(9924..10799)
                     /gene="aguB"
                     /locus_tag="VQ846_001418"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET3075988.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(10809..11894)
                     /gene="aguA"
                     /locus_tag="VQ846_001419"
     CDS             complement(10809..11894)
                     /gene="aguA"
                     /locus_tag="VQ846_001419"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET3075989.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALGRPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(11891..13018)
                     /gene="nspC"
                     /locus_tag="VQ846_001420"
     CDS             complement(11891..13018)
                     /gene="nspC"
                     /locus_tag="VQ846_001420"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET3075990.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLEIMDHIVF
                     NSERQLRKHGPRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGVDDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPSLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(13018..14277)
                     /locus_tag="VQ846_001421"
     CDS             complement(13018..14277)
                     /locus_tag="VQ846_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET3075991.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(14274..15134)
                     /gene="speE"
                     /locus_tag="VQ846_001422"
     CDS             complement(14274..15134)
                     /gene="speE"
                     /locus_tag="VQ846_001422"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET3075992.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            15390..16105
                     /locus_tag="VQ846_001423"
                     /pseudo
     CDS             15390..16105
                     /locus_tag="VQ846_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(16071..17546)
                     /locus_tag="VQ846_001424"
     CDS             complement(16071..17546)
                     /locus_tag="VQ846_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET3075993.1"
                     /translation="MKEVDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         5330 a         4168 c         3326 g         5353 t
ORIGIN      
        1 agtttgaatt tgattttcga agagtatcaa ccaccataaa agaggcagat aaaactacct
       61 cttatttacc tagttcttgt gttcgtttat aggcttgatg aactgcatcc atgaccgttc
      121 ctcgaaaagc atgcgcttct aggcttgcta cgccagcaat agtcgaaccg cctggactac
      181 aaacttgatc tttcaaaact ccaggatgtt gctggctttc taggaccaat tgcccagctc
      241 ctaccacagt ttgggctgcc attttcaagg ctgtttctcg tggtaatccc gtctgaacac
      301 ctgcatctgc caaggcctca ataaagagat agacaaaggc tggtccacaa cctgcaagac
      361 ctgtcgctgc attgattaag ctttctccta attcaaccaa gagaccagcc ttggctaaaa
      421 gctgacaaaa gagctcactg tcctcagccc tgcaattagg agacaaggca taactaatca
      481 ctccttgccc gatagaagca ggggtattag gcatcatacg aataattcgg tgttggcttg
      541 ggataagact tgctagtttt tctaaggtca atccagctgc catcgaaatc aaaagaagac
      601 tttctctttt ttcaaggatg gtctggtatt gagaaagcag ttcagaaaac tgagcaggct
      661 taacacctag aaaaatcaca tctgcttctt cgaatatttc ttcattgtta gaagtctgac
      721 caccgaagtt agcgatgaaa gcatccacct tgacttgact acgattggca aggagaatct
      781 catctgacgg tctagcctgc aagacagact tggctaaact agcacccata ttccccaaac
      841 cgataaatcc aatcttcatc ccttactccc ttatctgtcc atcaccagtg accacatact
      901 tgtagctagt caattccttc aagcccatgg gaccacgcgc gtgcaatttc tgagtagaaa
      961 tccccatttc acaaccaaga ccaaattgtc ctccatctgt gaaacgagtt gaggcattaa
     1021 catacaccgc tgcagagtcc acttgatctg taaagtatgc tgcagcttca gcattttccg
     1081 tcacaatagc atccgaatga tgagtgctgt gggattcaat gtgcgaaacc gcttcttcta
     1141 aactgctcac aaccttaaca gcaaggacat agtctaaaaa ctcggtgtca aagtcttggg
     1201 tctcagctgc ttgacctgaa acaaactggc ttgctttgct atccaagcgg aattgaattg
     1261 gttccagtcc agcttcctta cgctctgcaa ccagcatttg atccaagcga ggaaggatgc
     1321 ttgctgcctt gttttcatga accagcagaa cctccatggc attacaaaca gaaggacgac
     1381 tggttttagc attgttgatg atagacagcg ccttgtcttc gtctgcatcc ttatccacat
     1441 agacatggac aatcccagtc cctgtctcga taacaggtac aatcgcattc tcaaccactg
     1501 cattgatcaa gccagctcct ccacgaggaa tgagaaggtc tagatagccc ttggccttca
     1561 tcatagcata actactttca cggctagtat cctccaccag ttgaatcaca tttggatgaa
     1621 tagtagtcgt ctccaagccc ttcttcaagg ctgtgacaat ggcatgggtt gtttgatagg
     1681 catccttacc actacgaaga acaaccgcat ttccactctt aagagtcaaa gcagccgcat
     1741 cagacgtcac atttggacgg ctttcataga taataccgat gacacccata gctacacgtt
     1801 tttttgtgat aaccaaacca ttttcaagct gacttgtttc taaaacttca ccgattggat
     1861 ctggtaaggc aaccacttca cgaattcctc ttgccatcgc ttctatacga tctgcatcca
     1921 aataaagacg atccaacatc acatctgaga ttttcccctt agccgctgcc atatcgaggg
     1981 cattagccgc taaaatttcc tcagtagcag ccactaagtg atcagccatg gctagcaagg
     2041 cttggttttt cacttcttca ctagctgtgt taatcgattt tttaacagcc tgtacctgtt
     2101 caaattgttc ttgtctactc accatagttt acctctaaaa ttctgtaaaa agtagttgga
     2161 tttcaggagt aatggaaatc cagtcgtcac ggtaaatcaa gacacccttg gctttttgag
     2221 aacgcaacat atcctccaaa gcagatgctc caaattgcac gcgtcctttt ccaagtgatt
     2281 ttccactttc cttgtcaaat actgtcacga tatcaccgta agaaaaggct ccttctgctt
     2341 caacgatacc agataagaga agactctttc catgttgaga gagagcttcc gcagcccctt
     2401 tatcaaccca aatagaacct tgactctgag catagaaggc aagccattgt ttctgggtac
     2461 gaagcccctt ctcttgagca acaaagtaag aaccatcctc ggtctcctct gccgcctcaa
     2521 tcatggaatc tgatttcaag gatgagcaaa tataaacagg aactcctgat tccgtcgcga
     2581 tagttgcagc cttgattttg gttaacatac ccccagttcc gtttgacgaa cctgctccac
     2641 cagccatatc aataatctca cgattgatgg tctcgattct ctccaagcgt ttggctcttg
     2701 gatctgaatt aggatttcca gtatagagac cgtccacatc tgtcaagaaa actaaaaggt
     2761 ctgcttggac catcgccgct acttgagcac ttagagtgtc attgtcccca accttgagct
     2821 catcaataac gacactatca ttctcattga tgataggaat tgccccacgg ttgagcaaaa
     2881 ccgacaaagc ctgatgggca tttttataac gacgcttatc cacaaagtca tcttgggtca
     2941 gcaagatttg tgcagaaacg atttgacgca agagaagatt ggtcgtatat tcttccaaca
     3001 aaagtccctg ccctaccgct gctgaagcct gtttatcagc aattttagtc ggacgctttt
     3061 taaatcctaa ggctccaaaa ccagccgcaa tggcacctga agacaccaaa atcaactcat
     3121 gaccagcctc gtgcagcata gccaactgct gggtaatatc ctttacctta ctacgtgata
     3181 aacttccatc ctcatttgtc agagaagaag tacccacctt aaagacaatc cgtttgtatt
     3241 tcatagtctc actccgatta ttcatattta tccattataa catgaataac aggaaataga
     3301 aaagagttga taggaaaaag aaggccctaa aaccttcttt tctactactg ttctgattcc
     3361 gatttgttta cactcgtttc agagctttgt gaagttgagg atgtcaaatc ttgactagtt
     3421 gaagaatttg taacactttc atgttgactc gtttctttat tttcaagatc ctttttcact
     3481 acttctttgg attcttttat ttctgaatga cttgtagttt taattgtagc agatttctcg
     3541 atcggaacat ccactaacca ggcaccactt gaatccacag tatagccttc tggtgtctta
     3601 ccattcacaa gtaattgacc attttctttc aagaagtacc atttatcctt gtctttaatc
     3661 caacctatag ctcgtgaacc acctgtcttg tagaaatacc aatcatttgc ctttttaagc
     3721 cagccttgct tcatagctcc tgattcttct agatagtaat gatgtccagc aacttctatc
     3781 tcacctgtct tcatgatacc agtagaatcc atatagtacc aggcttcttt atcttttacc
     3841 cagcccgttt tcatttcccc tgatggagca aagtaatacc attgcccctt atattgaagc
     3901 caacctgtct tcatagagcc atcaacatcc aaataatacc actgctcttt atcttgaatc
     3961 caaccaattt ccatctggtt ttctttgtta aaataatacc agattccagc gattttcttc
     4021 caatttttag cagcaacacc agagtctgtc aaatagaacc aacgattgtt ccattttttc
     4081 cattgattct gtaataagat acccgtttgg ttaaagtaat accattcacc cttaatttca
     4141 ttccaaccga cagcatattc tcctgtagaa tcaggcgctt gataccacca gtttccatat
     4201 gaactcttat gccaaccagc ttgaaaactt ggaataggct tgtaggaagt tgaaatgttt
     4261 actaaaccgt cttgtcgaat atcaaaaact gttgcatcat agtctttgct ggctgcgttg
     4321 attctctcaa ttcctcgttc tttgagccaa ttaacatact cactatcaac accatttttc
     4381 caaggtagac tatccgaagt ttgaacaatc aaactcggac tcaaattttt aatgaaatcc
     4441 ttggtatttg atttgttggt atcatggtga tgattaaact tcatcaaatc aacttttcca
     4501 atgagaggac catacttgtc ttctgctcca tgaacattat ctaagtcgcc cccaaggtaa
     4561 attttcttgc cattgacttt taccacacta atcaaggaat tggaattatc atcccaaatt
     4621 ttctttaatt cacctgatga atcggtttca ttttcatagt tatagagttg aatatccatg
     4681 tccccaaact gaaaatgagc atctccctgt gtgatgtttt gaataactga aacacctttt
     4741 tctgaagcag tctgtaaaac cttatcataa ccgtacagat tatcccatag acgttcagaa
     4801 ttagtaatac gattatcact atatttttta agatagactc ggtcaactgg ataggtagac
     4861 agtaattcat caacatttcc aatatggtca ctgtgggtat gggtcaccaa aataaaatca
     4921 agtttttgga cacccaattc cttcaaacga cgaaagacac ggtctgttag aacatgctta
     4981 taagacgttt caattccttc tctccatgga tagcgagaat cacttccatc tgggaaatca
     5041 taatcttctc ctgtatccac catggcaaaa tgtccattgc tttcaagaat aatcgcatca
     5101 ctgccacctt cttgaacatt gataaagtgg attttatttc ctgaactttc ttgagcctga
     5161 acattcccat accatgacaa acctaaaaaa gcgcctacaa gtgctaaact agttaatttc
     5221 tttttcattc ttactttctc aatctctcca gggtttcctt aaaaatttct gggatatctg
     5281 ctttaaattc caaggtctta cctgttctcg gatgagtaaa acctaaagtc ttggcatgaa
     5341 gaaattgtcc atgtcctttc aaagtcttgc gaggaccata gacctcatca ccagcgactg
     5401 gatggtcgat ataagccatg tggacacgga tttgatgagt gcgccctgtc tccagttgca
     5461 actctactaa gctataatcg ccaaagcgtt ccaagacatg aaaacgcgtc actgcaggct
     5521 tccctttagc aattacagcc tgtttcttac ggtctttttc actccggcca atcggcgctt
     5581 caattacacc acgatcatta ggcagatttc catgaacaat cgcccaatat ttgcggagag
     5641 actttttatc cttgagttct tgggcaagtg ctagatgcgc atcatcgttt ttagcaatca
     5701 tgagaagacc tgacgtatcc ttatcaatac ggtgaacaat tcccggacgc agaaccccat
     5761 tgataccaga caagtcctta atatgataca tgagggcatt gactagagtt ccactagtat
     5821 gaccagcact cggatgcaca accattccct gaggtttgtt aacgacagcc acatcctcat
     5881 cttggtagac tatttctagc ggaagatcct cagccacata ctctaatacc tctggttctg
     5941 gcacatggta agtgacgaca tcaccctctt ggactgtgta tttagctttc ttgacttgac
     6001 cattgaccaa gacctggcct gatttaattt gttcattcgc aagactgcgt gataattctg
     6061 tcaaatctga caaagcctta tccaaacgca gaccaccagt ttcaatttta atttccattt
     6121 atttcctctt ttagcattgc aatcaataaa ataatcactc caaccgtcag atagctatct
     6181 gccacattga aaattgcaaa gttgataaag tcaaggtgga acatatccac aacaaagccc
     6241 tgactggccc tgtcaataaa gtttccaaga ccacccgcga ttatcaaagt caaacccaag
     6301 accatccaga atgagtcctc catgtgttta tgtaaatacc aaatggcacc tatcacgaca
     6361 accagagtaa tgacagcgaa taacagctgc tgatcttgta agatagaaaa ggctgcacct
     6421 cgattttgca ggtaggtcaa gctaacgaaa ttgggaatcc aggagcgcac ttcacccagt
     6481 ggaatctgct ggacgatata ggatttgacc aactgatcca gcccaattaa aagcagtaca
     6541 atgactgcca ctattgctct ttttttcatg atttcctctt ttgatcaaaa tattcttgca
     6601 tgacttctac gaagagagtc ccagcttgac taagctccac ttcttcacgt ttaacataga
     6661 ccatgcggtt atctaggtta tccttgagac gaataactgt aatgccatta acactgtcac
     6721 tatctaaaaa tccagaacct gtcgcatagg cgtccgtccg ctccaaaata ccattcaagg
     6781 tggcacggtc tgtcacatta aacatctgtg agctagcgct ggtatcgaca aagttctctg
     6841 aataataaag gtactcgtct ttctcttgag tgaaacgaac cgttggtaaa tccgctaaat
     6901 cctccatgac taattcctct ttctgggcta aaggatgacc ctcacagaga taaatatggg
     6961 tatggaaagg aatcaattcg atgacctcca gacctaactt ttcaacccgt tgcataatcc
     7021 cctttttatt ttgattgttg aggtagataa tcccaatctc actatgccct tgcgccactt
     7081 catctaatat ttgaacagta gttgattcaa aaatacggaa gttcttatag tcaggatagc
     7141 gctctgaaaa ggccgtaata gttggtggta agaagtcata gtgctggcta gcaacggaaa
     7201 attcatcttt ttcttcttca ggattggcat actgattttg aaaaatatca aatcctttaa
     7261 ccaattcttg cgctttttca taaaattcca tcccacgacg ggtcaagaaa gtccctgagc
     7321 tggtccgacg gaaaatctta aagcccaact ctttttccaa atcacgaaca gaaatagaca
     7381 gactcggctg actaacatac atcttttcag cagcttcacg aaaagtacca ctattggcaa
     7441 tagccacaac atagcgtaat tgttgaatgt tcatcttcta cccccaactt ctttatcttt
     7501 tcattatacc atattttaga agttttccaa aaaggaaaaa agaacatcct attcttctta
     7561 actatcttca ctatctgcct ttctcacgcc aatcttattt tcaaaatcaa ttcaaaaaaa
     7621 taagtggcta cacaccactt cagtatcgga aagaaaaacg ttgactattt gtgaaaaaag
     7681 aaaatgccgg aaaattccga cattttttta gtaagctaac ttcctgaaaa taggagtaac
     7741 cacacatccc agaaggcagt gatattgatg agaatatgaa ctagtattgg atagtagaga
     7801 tttttagtca tgcgatacaa tagagctaaa ataagacctc cactagcata aatgaaaaaa
     7861 gcaagaggag ttaaagcaaa attgatatga atataaccga aaataatagc agaaagcaaa
     7921 acatctccgt accaaggtga gtttttgaaa aaggttgtca taagcacacc tcgataaatc
     7981 aattcctcag caataggggc gatgaagcaa acgatgagca agaaataagg gaactcctgt
     8041 ctccccatca tttctaccgt ttcattcaaa gaaatctgat ttgaagacag ggatatgaaa
     8101 tacgaaaaga ggaagtcaga catatatgaa atgatgtagc ccagtaaaag gtagatgaag
     8161 tacctcagct gccacttgaa atgaaaaatt ttctttcctg caaaaaccag tagatagatg
     8221 accgctaaga aaagaactcc gctctccatc aaaagcagaa tctgaaaaaa ttcacgactt
     8281 gctggtagat agggctttgc gaggtgattg aaaactctcc aaaaccaagt tgatttgtaa
     8341 aaaattaagg acaatgctag taaaatttga atagcccgtt ttttcatatt aaattctctg
     8401 ctttctccac ttgtttcttc atattactaa taaattttac ttaaaatctc gcagcacttc
     8461 ctttgcaaag atgattgcct cctccaatat atcctaaatc ataggtgcct ctgggacaaa
     8521 atccatgaac cggcagagtt aatactggtt ctatttccca atcgtaccta ccattaggat
     8581 ttttaccata tggatctgct ccaccttgta tcagctctaa ttcttcattt gttagattta
     8641 tattttttgt cattaaaatc atgatatatt tcctccatta ttcttcagat tagttgagta
     8701 atctgatact catacctact tacaaaaaaa ctattatatt aagttggttt tttaatttgt
     8761 ctagttgcaa cattgacaaa catagtatag catatctttg ccaaatatcc tcttcaaatc
     8821 atgaattgtc atcaaaacat cttaaactat aaaatcaatt agtctcaagc tttctatcaa
     8881 tttcttctcc aaaatatgct ataataatag caaaagataa agaaggaaga cctatgatta
     8941 aactactagc cttggatatg gatggaaccc tcctcaatga agccaaggaa atcccacagg
     9001 ctcacatcgc tgctattcac caagccattg aaaaaggtgt caaactggtt ctctgtacgg
     9061 gtcgccccct tttcggtgtc ctcccctact acaaaaaact gggactcgac ctccagaatg
     9121 agtatgtgat tgttaacaac ggttgttcaa ctcaccagac tagcgactgg ggcttggttg
     9181 actggcaaga acttagtcca gctgacatcg aatacctcta tgaccttgct gaaaagagtg
     9241 atgttcagtt gacacttttt gacgagtcac attattttgt tctcggtggc aaacccaatc
     9301 aagttattga aaatgatgct aaactagtat tttcagacct gactgaaatt tctcttgaag
     9361 aagcgactag tggaaagtta cggatgttcc aaggtatgtt tttaggaaca aaagaacaaa
     9421 cagacgattt tgagcagcgt tttgctgaag agctttgcca acgattcagt ggagttcgtt
     9481 cgcagcctgt catttatgaa gcaatgccgc ttggaacgac aaaggctact gctctttcac
     9541 gactagctga gattttgaag attgattcct cagagattat ggccatgggc gatgctaata
     9601 acgatatcga aatgctccag tttgcagggc ttgggattgc aatgggaaat gccagcgatt
     9661 atgtcaaatc tcttgcggat gccgttacct caagcaacga agaagacggc gttgcgcgtg
     9721 ctattgagaa atatattcta taaaaaagaa aagcaaatag acagaagtta ctgctatttg
     9781 cttttttgct aatattttaa aatacggact aatcaataga caagaatagc gaatcataaa
     9841 aatcgattta ctagatgcca tagtatctta tagttgctaa taagaagtta gtctagcaga
     9901 atgaatctct catttctccc acactaatct gtaatttgtc tatacatttc tggtcttcta
     9961 tctcgaaaca agccccagtt taggcgttca cttgctccct tgtctaggtc ataagtagct
    10021 aacagaacag cttcttcttg tctttcagct cgttctagaa tagctcctgt ttcatccgtc
    10081 ataaaggagg aaccgtagaa gtcaagactg gaactctgtc cgccattttc ctcactagga
    10141 gtaacctcct ctaaaccata acgattggct gcgatgactg gaacaatatt cgctgctgcg
    10201 tgcccttgca tagtacgttg ccagtgacca caactatctg tatccaaaat cggctctgaa
    10261 ccgatggctg ttggataaaa gagcaattct gccccattca gagcaagaca gcgggctgtt
    10321 tcggggaacc attgatccca acagataccg ataccaatct tagcatagcg agtattccag
    10381 accttgaaac cagtgttacc aggcgtgaaa tagaattttt cttgataata atggtcatct
    10441 ggtatgtggg tctttcgata aacgcccagc acttccccat ctgcatcaat gacggcaata
    10501 gagttataca agacattacc atctttttca tagaaactga ttggtaaaac aacttgtagt
    10561 tccttagcaa tcaccttaaa atgctgaatg gcagtatttt ccgctacaga ttgggcatac
    10621 tggtagtagt catactgacg ttcctgacag aaatagggat gttcaaacaa ctcgggcaag
    10681 agaataattt gggctccttg ctcagcagcc tgacgtacta aacgctctgc ggtttggata
    10741 tttgttgcca catccttagc gcattgcatc tgaatggttg caactcttac atttctcatc
    10801 tttttctcct attctggaat ttgttgggtg atacagtgga tattgccacc acctaagaga
    10861 atatctctgg ctggtattcc gacaacttta cggtctggga aacacttgct gaggatatct
    10921 aaggccactt ggtcgtttac atcctcaaac tgtggaacca agacagcctt gttggcgata
    10981 taaaagttta cgtaggaagc tgctagtcgt tcacctgcgt atcgcttttc ttctccttct
    11041 tcatagatgt agcctggcaa atcttcttct gtcacaactt gtcgaactgc agggataggc
    11101 aatttatgaa tggtgaagtg acaacctttt gcatctgttt cctgttctaa gagttcgaga
    11161 tctgcttttg acatggcata ctggggatca ttttcgtcat ctgtccaagc caaaacaagc
    11221 tcagcaggac caacaaaggc agcaacatta tcgacgtgtt cattggtttc atcctgataa
    11281 ataccataag gaagccaaat aactttttca gcaccaagac tttctaataa tgtgttttca
    11341 atctcctctt tagtcaggtt aggattgcga ccaggactaa gcaagcaact ttcagttacg
    11401 agaatagttc cttgaccatc gctatggatt gcgcctcctt ccagtacaaa aggtttagca
    11461 tcatagacag gccttcccaa ggcctcagca aaacgactgg ctacttggtc atcctcttca
    11521 taatcttgat aaagaccatc ataggtgcct ccccaagcat tgaaggccca atccacggct
    11581 aatttcttac ctttatcatt gacgagaatg gttgggccag tatcacgcgc ccaggcatca
    11641 ttggtgggaa tgtctaaata aacaaccttg tctccaagat aggattgggc ttcagataga
    11701 taggcctgct ccaccaaaag atagactctt tccccttctg ctatggtctc gatgatctga
    11761 gtaaatgctc ttttagcagc ctttccttga aaaggccatg atcctggtcg agtcggccat
    11821 atcatgaggg taccatgatg gggttcgtac tctgctggca tgtgatagcc taattttttt
    11881 ggactgtcca tcatgataat ctccctttaa agtcttgata gccaaaagct ttgagtaagc
    11941 tacagtctcc ctgttcgtcc atgagataga gacttggcaa tccaatacca ttaaaggtat
    12001 tatttttgac aaaagaataa atggccatgt cttgaaaata aagtctgtct ccgatttgga
    12061 ctggattttc aaaactataa tcaccaatca catcgcccgt cagacaggta ttagaagaaa
    12121 gtctgtaggt atgggctttt tcctgtgcct caaagccatt tctcaaagat ggacgatagg
    12181 gcatctcaag tacatcaggc atatggcagg tcgcagaggc gtctaaaacc aagatttcca
    12241 taccgttttc tacaatatct aatacctcag ttgctaaata acccgcatta agcgcaatgg
    12301 cttcaccagg ctcgatatag atttcaagat tgtaagtttt tcggatacgc ttgatttctg
    12361 aaatcagcaa atccacatcg taaccttctc ttgtaatatg atgaccacca cccatattga
    12421 gccattttac ctcatgtaag tagggaccaa actgttcttc tactgctttc aaagttgttt
    12481 gtaaatcatc tactccctgc tcgcaaaggg tatgaaaatg aagtccgtca accaaatcta
    12541 gcaaatcact cggaatcttg tctatagtaa ctccaaagcg agaacctggt gcacaagggt
    12601 catagagcgc gtgatctcct tgagttgaac actgagggtt gaggcgcaaa ccaacactga
    12661 caccagcctc tcgacaacgc ggaccgtgtt tacgcaactg tctctctgag ttaaagacga
    12721 tatggtccat tatctctagc aattcctcca agtctgcatc cttgaaagca ggcgcaaata
    12781 catggacttc accaggaaat tcttcccttg ccaatttggc ctcatagagt ccactagctg
    12841 tcgtacctga tagatactgg ctaatcaagg gataagtttt gtagagggaa tatgccttct
    12901 gggcaagcaa gaccttgcaa ccggcctctt cttgtacata ttgtagaatg cggcaattag
    12961 cttctaactt ggccaagtca ataacatagg ctggtgttgg tacttgttct aacttcatta
    13021 gtccaccatt tgtggatttt caaccacaac ccatggcaaa ccatactcat tcaaagcttc
    13081 catgaatgga tctggatcta actcctcaag gttatacact ccagcttgtt tccaagttcc
    13141 gttcatgact aattttgtcc caatcatggc tggaacgcct gtcgtgtatg aaattgcttg
    13201 tgacccgact tctgcatagc actcttgatg gtcgcaaaca ttgtagatgt agatagtctt
    13261 ttcaacgcca tctttgacac ctgtaaagat acatccaata ttggtttttc cgactgtacg
    13321 tggcccaaga ctggcaggat ctggaagcaa ggctttcaaa aattgaattg gaacaatttc
    13381 ttggccgtta aagttaatgg tatccgtacg aaggagtcca acattttcaa gacatttcat
    13441 gtgcgtcaag taagattgac caaaagtcat aaagaaacga atgcgtttga cacctggaat
    13501 gttcttggcc aatgattcga tttcttcatg gtgaaggaga tacatatctt tttgtccaac
    13561 ttgagggaaa tcatactcac gcttgataga catagcttcg acttcgaccc atttcccatc
    13621 ttcccagtaa gaacctggag cagaaacctc acggagatta atttctggat taaagttggt
    13681 tgcaaatgga taaccgtggt caccgccatt acagtctaaa atgtcgatat aatggatttc
    13741 atcaaaatag tgtttgaggg cataagctga aaagacacta gttacacctg ggtcaaaacc
    13801 agaaccaaga agagcagtca agcctgcttc tttgaatttc tcttgataag cccactgcca
    13861 tgagtagtca aagtaggctg taaaaccaag ttccttacaa cgtttttcgt agatagcacg
    13921 ccactcaggg tcttctgtgt cttctgcttc gtagttggct gtatcgatat agtgaacacc
    13981 tgttgccaaa caagcatcca taatggttaa atcttgataa ggcagagcta cattcaaaac
    14041 ggcttctggt ttgtagcttt caatcagggc aatcacttct tcaaccttgt cagcatcaag
    14101 tgctgcagtt tcaattttag tacttgtttt gccttctagc ttcgctttca agtcatcgca
    14161 ttttgacttg gtacggctag caatcataat ctctgtaaat gtttcgctat cttgacaaat
    14221 ctttgaaata gcaacttggg caacgccccc acaaccaata actagtaaac gactcatttt
    14281 tttccttcct cttcttctaa aatgtcctca acatacttgg gcaacataaa ggatcccacg
    14341 tgtaagtttg cagtgtagta ttctgtgaaa agctggcgtt ttttccaacc ttccttgtca
    14401 aaatctttga cagggtggta ttttttcgat gcaaatccaa acaaccaata gccagctggg
    14461 ctagttggaa tatgggcctg ataaacccga ctgattggaa aggattgatt gaccttgcgg
    14521 tgcatgcttc ggcaggccga ctcatcctcg tcaaagaagg gactcccatg ctggtaaatc
    14581 atgatgccgt cttccttcag agctcgataa ctattgccgt agaattcctt ggtaaagagt
    14641 ccttccgtat ggccaaatgg atctgtcgca tcgttgatga taatatcgta atcatcttcg
    14701 cagtttcgca aaaagcgtag cccattttgg tagtaaatgg taacacgagg atcatctagc
    14761 cctgcagcaa agtctgggaa atactcacga cagacctcga ccaacatctc atccggttcc
    14821 acgatatcga tttgttccag ttcaggatag agtgttaata cttgggcaac accgccgtca
    14881 ccacccccaa taaccaatac tttctttgga tttgggtgga cagccatggg aacgtgaacg
    14941 gtcatttcat tgtagacgaa atcatccgca tctgagaaca agacatggcc atttaaaatc
    15001 agtattttcc caaaagctgg cgtatccaag acttcgatat cctgccattc actttttcca
    15061 gcgtaaagtt gcttggctgt tctcagagac aatttgacat ctggagtatg aacttcagaa
    15121 aaccataaat ccatctattt ctccttatat tatatttagc gcagttagct actgccaaag
    15181 cccaagtggt atacttggaa taagccactg tggattagtt tattccctcc aatcgcttga
    15241 cgatttccga aaggctctaa ccacagtttt ttgtttcaat tggtaatgag tttgataacg
    15301 catagacgtt ttatctcgtc taaggtaaca agacaaaaaa ttcgtggata accaagtaga
    15361 cttgaaatac tacatctgga ggtactccca tgctttatgt gggcattgat gtcgctaaaa
    15421 ataaacacga tgttacagcc ttgaatgttc caggaaaaac tgttcttaaa ccactcactt
    15481 tttcaaataa taaagctggt tttgaactct tagatctgtc tcttcgacaa ctcaaccaag
    15541 actgtctcat cgctcttgaa aataccggac attatgcctt taatctactg aacttcctgc
    15601 atgaacaagg atataaagtt tacacctgca atcctttgct catcaaggaa tttgccaaat
    15661 cattatcact tcgcaagact aaaacggata agaaagacgc tcatggcatc gctcttaaac
    15721 ttctttctga ccccaatcgt gaacaatttc aacacgataa tcggcaagta gaactaaaaa
    15781 tactggctag acatattcat cgtctcaaga aaaacagtct gattggaaag tacaatacac
    15841 tcgttgtctt gatatcatct ttcctgagtt ggataaaatc gttggaaagc attcagaata
    15901 tacctaccaa ctcttgacac gctaccctaa tcctcagaaa aggattgagg caggatttga
    15961 taagctgata gaaattaagc gattgaccgc ttctaattca agggcttttt gccgtgctct
    16021 aaaatcatcg ccaaatcatc aaattgaaat aagaaatagt ttagataaaa ctattgactt
    16081 ttcttatagt ttgtctttct cttaataacg ttgatatgat tgacctctgg atcttccgtc
    16141 ccttggaggg agcaaccacg ttccttggcg aattggatat agtctacaat ttctcgtgta
    16201 atgcgttcac caggagccaa gataggaatc cctggaggat agcacatgac aaattctcca
    16261 cagacctgtc caacagactc atctaaggtc aaactttttc tctctgaata gaaggcttct
    16321 tgtggagaca gcaccaactc gggctgaata tattctccag ctattaagtc cttcccatct
    16381 cgtgaataga gtctcttaat atcagccaga gcaccaacca agcgctcgat gtcttggatg
    16441 cggtcgccga tggaaatata ggccaagata ttgccgatat caccaaactc gatctgaatg
    16501 tcgtattcgt ctcgcaagag gtcataaacc tcgatacctg ttaagcctat tccctgagtg
    16561 taaactgaca acttggttac atcaaaatca cagaccgaca caccatctat taactctttt
    16621 gagtaagcat agtaaccgcc aatggcattg atttcatgac gagcgtactc ggatagctca
    16681 atgacttcct caaacgactc tttaccacga agggccaagt tgcgacgtga aatatccaaa
    16741 ctagccatca ataagtaaga ggcagatgta gactgggtca ggtttataat ctgacgaacg
    16801 tactcgggat tcatctgctc cccgattaaa agaagcgaac tttgtgtcaa actcccacca
    16861 gacttatgca tggaaactgc tgccatatca gctcctgcat ccatagcaga aattggaagt
    16921 ttaccagtga aatgcaaatg cgctccatgg gcttcatcta ctaaaaccat catgccagct
    16981 tcatgagcca tttctgtcaa cccctttagg tctgaacaga tgccgtagta agtaggattg
    17041 ttgattagga tagccttagc atctggatgg tcctttatgg cctgtgctac tcggtcattt
    17101 tcaagaccta aagcgatacc aatcttagga tctacactca tctcgatata gatgggaatg
    17161 gcaccacata gaaccaacgc attgatagca gatttatgga catttcgtgg cagaataatc
    17221 ttatctcctg ccttgcaggt tgccagaatc atagtctgca ccgatgaagt tgttccacca
    17281 atcattagaa aggcatggct agctccaaaa gcatctgcag ccagctcctc tgcatcacga
    17341 ataatcgaaa taggatggcc taaattatcc aagggtttca tcgaattgac atcaatgcct
    17401 acacattttt ctcctaagag ttcgacaagt tctggatttc cccgtccacg cttgtgacct
    17461 ggaacatcaa agggaacaat ccttttcttg cgtaacttca ccaaggcctc ataaattggg
    17521 gcttggtttt gatctacctc tttcaaaaca tactcctttc aatattttta ggaccttatg
    17581 aaaactaagg aactaaaggt tgactcatga aaaaagagca ggttttcacc tgctctgcaa
    17641 tcttctgacg tgtttatttt ggtttctgtt gagtatgtct gttcccgatg tttcctaact
    17701 ctctagtagc aaatattacg tactttattg atcgtttcac aagatgacgt gtgctttcac
    17761 cacactaaaa atttatgaat taggacaagt gtcctaacat caacttataa aagtaggctt
    17821 ttaaccctat caataatgtg gcttttcaac cacgcttcgg cattcaaccc tgcctgtccg
    17881 taggcatttt ttactcgggt ttatatttgc tagaaaacat catctcattt tctaagcttt
    17941 attactatat catgttttta agaacttgta aagtattttt tgataaaaaa caaaaatata
    18001 atagattgaa actagaatag tacacttctg cttctaaaac attattagaa atagatttta
    18061 ctgtcctgat cgatttgttc tgttcttgtt tcattttact atatgttcgt aaatgaaagt
    18121 tctataatga agttagccac ccagccctcc taaaaattag atggttcaac tagctga
//
LOCUS       DAVBNO010000037        18138 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVBNO010000037 DAVBNO010000000
VERSION     DAVBNO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18138
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.037"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(<1..839)
                     /gene="lytB"
                     /locus_tag="VQ846_001425"
     CDS             complement(<1..839)
                     /gene="lytB"
                     /locus_tag="VQ846_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HET3075994.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYTENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQA"
     gene            complement(881..1867)
                     /locus_tag="VQ846_001426"
     CDS             complement(881..1867)
                     /locus_tag="VQ846_001426"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET3075995.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            complement(1830..2630)
                     /locus_tag="VQ846_001427"
     CDS             complement(1830..2630)
                     /locus_tag="VQ846_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET3075996.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            complement(2880..3260)
                     /locus_tag="VQ846_001428"
     CDS             complement(2880..3260)
                     /locus_tag="VQ846_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET3075997.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            complement(3318..3677)
                     /gene="rplT"
                     /locus_tag="VQ846_001429"
     CDS             complement(3318..3677)
                     /gene="rplT"
                     /locus_tag="VQ846_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET3075998.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            complement(3729..3929)
                     /gene="rpmI"
                     /locus_tag="VQ846_001430"
     CDS             complement(3729..3929)
                     /gene="rpmI"
                     /locus_tag="VQ846_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET3075999.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            complement(3962..4492)
                     /gene="infC"
                     /locus_tag="VQ846_001431"
     CDS             complement(3962..4492)
                     /gene="infC"
                     /locus_tag="VQ846_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET3076000.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     misc_feature    complement(4508..4639)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(4805..5989)
                     /locus_tag="VQ846_001432"
     CDS             complement(4805..5989)
                     /locus_tag="VQ846_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076001.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIESWGSSRKKEVFN
                     RS"
     gene            complement(5993..6580)
                     /locus_tag="VQ846_001433"
     CDS             complement(5993..6580)
                     /locus_tag="VQ846_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3076002.1"
                     /translation="MLQLTHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNIAAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDVCDRVVTIHQGQIEEV"
     gene            complement(6613..6801)
                     /locus_tag="VQ846_001434"
     CDS             complement(6613..6801)
                     /locus_tag="VQ846_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076003.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSWSRYDSYSEYKVG
                     GGWNYGRYEVINYYSGGY"
     gene            complement(6980..9220)
                     /locus_tag="VQ846_001435"
     CDS             complement(6980..9220)
                     /locus_tag="VQ846_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET3076004.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSFQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKENVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQQGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGVDSW
                     KIESVR"
     gene            complement(9204..9854)
                     /locus_tag="VQ846_001436"
     CDS             complement(9204..9854)
                     /locus_tag="VQ846_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET3076005.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            complement(9922..10491)
                     /locus_tag="VQ846_001437"
     CDS             complement(9922..10491)
                     /locus_tag="VQ846_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3076006.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            10668..11779
                     /gene="ald"
                     /locus_tag="VQ846_001438"
                     /pseudo
     CDS             10668..11779
                     /gene="ald"
                     /locus_tag="VQ846_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            complement(12146..12406)
                     /locus_tag="VQ846_001439"
                     /pseudo
     CDS             complement(12146..12406)
                     /locus_tag="VQ846_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(12409..12909)
                     /locus_tag="VQ846_001440"
     CDS             complement(12409..12909)
                     /locus_tag="VQ846_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3076007.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            complement(13089..14057)
                     /locus_tag="VQ846_001441"
     CDS             complement(13089..14057)
                     /locus_tag="VQ846_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET3076008.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQDKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(14143..14358)
                     /locus_tag="VQ846_001442"
     CDS             complement(14143..14358)
                     /locus_tag="VQ846_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET3076009.1"
                     /translation="MRKSFYTWLMTERNPKSNNPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            complement(14367..15221)
                     /gene="cvfB"
                     /locus_tag="VQ846_001443"
     CDS             complement(14367..15221)
                     /gene="cvfB"
                     /locus_tag="VQ846_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET3076010.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTSLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(15281..15838)
                     /gene="frr"
                     /locus_tag="VQ846_001444"
     CDS             complement(15281..15838)
                     /gene="frr"
                     /locus_tag="VQ846_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET3076011.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVV
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(15847..16584)
                     /locus_tag="VQ846_001445"
     CDS             complement(15847..16584)
                     /locus_tag="VQ846_001445"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET3076012.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(16668..18002)
                     /gene="trmFO"
                     /locus_tag="VQ846_001446"
     CDS             complement(16668..18002)
                     /gene="trmFO"
                     /locus_tag="VQ846_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET3076013.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
BASE COUNT         5188 a         4136 c         3375 g         5439 t
ORIGIN      
        1 gcttgactat gagaatcgta gacccattct ttttcagcta ttttcccatc aaatttgaga
       61 taaaaccaag attccttatc ccaaatccat tcattggctg ccatgtagcc accagatttt
      121 agataataat agtgaccatt ctcgaaaatc cattctttat cagcatagtt tccattttct
      181 tttatgtaaa accaagattg gtattgtttg tcaaaaagcc aaccttgctg tactttggca
      241 ccactagcat tcacataagc ttgattaatc cactgacttg ttagtagata accaccggat
      301 ttgaaataat agtccttccc tttaatttgg agccattctt tctctgcgtg ctgtccatct
      361 gctttgatat aaaaccaagc atcgtattga gaatcataga cccagtcttc tattactttg
      421 gcacctgttg caccaacata ggaagttcct acccaagcat ttcttttcat ctttccatct
      481 tggtcaagat aataaaaggc tcccttgtct tctacccatt ctgatttggc catatagcca
      541 ccagatttga ggtaaaaata gtcgtcacct tgctttagcc attcattttc agtatagtta
      601 gcatctgctt ttatatagaa ccaagattga taatgagtat caaaaaccca ctcattcgct
      661 gcttgactgc catcttcttt cagatactgt tttccttgcc aagtaccatc actagccatt
      721 gcaccctctg gactagctaa gaaaaatagc agagctaaaa aaataaatct tactttcttc
      781 attaaccttc ttcctctgtt cttatttatt ttattgtacc aaaattctcc taaattcata
      841 ttacaaacta ataaaaaatc aagaacagat tgattgcagt ttacctcaga gactctttta
      901 cttcctgacg gagttcttcc agactgctaa taccgtattt atccatgact tttggtaaat
      961 tttcgatgat gtcagggcag gcataaggat tggtaaagtt agctgttcca actccgatag
     1021 cagatgcccc agccagatac atttctaggg cagcttcagc cgaatccact ccccccattc
     1081 caatgatagg caggtctgtt gtttgggcta cttggcggat gagtttgagg gctactggaa
     1141 agactgctgg acctgacatt ccacctgttc cattggccaa gattggtttt ctggttttga
     1201 ggtcaaagcg cattccaacc agagtattga tcatggtcaa gccacttgct cccgcatctt
     1261 ctgcagcttt tgcgacagta acgatatcgg tcacactcgg ggttaatttg acataaactg
     1321 gcacttctga ggcttccaca gctgctttca ccacatcata agccaaatct ggatcttgac
     1381 caatcaaaag tccatgatta cagtggtcaa cattgggaca agaaatattg agctcgatag
     1441 cttttacatt agttgccttg gaaatcccat gagaaacagc tgcatactct tgttttgaaa
     1501 aaccagctac attggcaata ataggaagat ttggatattc tctttccagc caaggtagct
     1561 tttcagccaa aacaacctct aaaccaggat tttgcaagcc aattgcattg agcataccag
     1621 caggcgtctc tgctactctt ggagttgggt tcccaaaacg tggttcaagg gttgtcgcct
     1681 tgatcataat agaacctaaa aggtttaaat catagtactt ggcatactct tgtccaaagc
     1741 caaaacaacc tgatgctgga ataatcggat ttttcaaatc caaaccaggt agagacactt
     1801 gtaatcgatt tgtagtcata attttctcct tataatacaa ctgttcctgt gcggaaaaca
     1861 ggaccatctt cacagacgcg ttggctgacc gtctcgcttt ctggtacttt tagaacacag
     1921 gcatagcaag ctcccatccc acaagccata cgagattcca gagataaata ggctcttggg
     1981 tgatcatcaa aggtttgatt gatatacttc atcattcctg gagccccaca cgagtaaaca
     2041 gcatcaaact gactatctaa atcattgata acaacggaaa catttccctt gatgccataa
     2101 gaaccatcat ctgtcgttac aaagacctga ccatactgag ccaattccgt tttcaaaata
     2161 acagcatcct tattagcaaa accgaggact gtcactactt tcactccacg ttcatgcaat
     2221 tccttggcca cctcaagcaa gggtggaaca ccaatcccac caccaacaag gagaacctga
     2281 ttctgctcat caaggtcaga caagtcaaaa ccatttccct gaggccccat cacatcaaga
     2341 gtgtctccct gacttaaggt tgaaaaaatt gcagtcccag ctccctcaat ccgataaatg
     2401 aggtgacact gcttgtttgc cttgtcaata gacgaaattg aaataggacg acgtaagaga
     2461 tgggcatcgt ccggtacacg cagatgaaga aattggcctg ctcgcatggc ttcaaccatt
     2521 tctccttcta ggactaattc aaagattgct ggcgcgattt cctcttgtgc aaccaccttc
     2581 atggtttcca accgaatgac acccaaacgc ttcttacatg tgggattcat aacttttcct
     2641 ccttaaattt taaggggacc agataaagaa aagaccttgc tagtgcaagg cctcagttaa
     2701 aatcgtacaa cacaagagag cacactcaaa aagtctcctc tagaaaattg cactattctt
     2761 ttatctgaca ccttgtgagt ctctctggac tccccttaaa ggttgaattt ttattagtat
     2821 actctttcag ctaaaaaaag tcaagtagaa aacgaatatt ctacttaact tgcacgaaat
     2881 tatttttcac gaatgacttc gaccttatat ccatcagggt ctttgacaaa gtaatagttt
     2941 ggtgtagttc ctggtagacc atttggctca gtcacttcat agccttttgc actgtgttct
     3001 tgatgaagtg cctcaagatc aggtgtactg agggcgatat gggcaaaccc atcaccaacc
     3061 acatacggac cgtgatcgta gttataagtc aactccaact catagtcatc accctcaaga
     3121 cctagataga caatcgtgaa ggcatggtct ggaaaatctc tgcgacgcaa ttctttaaaa
     3181 ccaaaagcat cttgataaaa tgcgattgat ttttcaagat tttctactcg taagcaagtg
     3241 tgtagcattt ttgaagccat atttttctcc tttattttta aaaagactgg acaatcctgt
     3301 tccagtctca tcagttgtta tttaccaagt tttgctttag ctgcatctgc aagagctgtg
     3361 aaagctactg catcgttaac agccaagtca gcaagcattt tacggttaac ttcgatctca
     3421 gccaatttca aaccatgcat caattgtgag tatgaaagtc cgttcatacg agctgccgca
     3481 ttgatacgag tgatccacaa tttgcggaag tcacgttttt tctgacgacg gtcacggtat
     3541 gcatagtagt aagagttcat tacttgttct tttgcagtac ggaacaagat gtgtttagct
     3601 ccatagtaac cttttgctaa tttaagaata cgtttacgac gtttgcgtga tacaacgcca
     3661 cctttaacac gtgccatgtt ttatttcctc caaatatttc ctagaattgt ttacttacag
     3721 tcaagctatt atttcaagcg agtaagcatt gctttgatac gtttgtaatc tcctgaatgc
     3781 accatagatg ctttacgaag atgacgacgt tgtttcttag tttttccgtg gaaacggtga
     3841 gaagtgtaag cacggaaacg tttaagtcca ccagaacctg tacgtttgaa acgtttagct
     3901 gatgcgcggt gtgttttttg ttttggcatg attttttctc ctttatttaa ctttctgaca
     3961 attatttttt gtcagtcgct ggcgccaatt gcatgaacat ttgacgtcca tccattttag
     4021 cacgttgttc gatgatggca atatcttgag ttgcttcagc aaactcggct aaaacttttg
     4081 caccaatctc tttatgggta atcatacgac ccttaaagcg aatagatacc ttaactttat
     4141 ttcctttttc aaggaactta cgtgcattgc gaagttttgt atcaaagtca cccttgtcaa
     4201 ttgttggact tagacgaact tctttcacag taacaacgct ttgtttttta cgttgttctt
     4261 tttgcttctt ctggtactca aatttgaact taccgtagtc cataattttt gcaacaggcg
     4321 gtttggcttg gggttgaatc aatactaggt caacattagc gttatcagcc aaagcttgcg
     4381 cttcactgag tggcttgatg cctagctgtt ctccttcaag accaatcaag cgaacttcac
     4441 gtacacgaat ctcatcattg atgaataagt cttgctttgc tatggttttc acctcttttg
     4501 ttttattaga gaaaaacaat agcggactcg taaatataca agcccgcacg ttatgatagc
     4561 gtttcttcga aacttttcat ccgtagggcc aggcaactta atgtcacaag gcgagaagct
     4621 ctcacttctg cttttctcaa cttctatatg ataccaagtt ttctagctct tgtcaagata
     4681 aaaagttatt ttttgaaaag tttgtgtttg tgtttgtatt ttggtttggt tttcgaatca
     4741 aaaagagaga atcaagttga ttccctctct atgttagttt ttccttaact tccttataag
     4801 aatgctaaga cctattaaaa acttcctttt tacgtgaact tccccagctt tcgataaata
     4861 gaatccccac tagcaaaagg ataatcaggc aaggaagtaa gaccatcccc atgctccaat
     4921 ttagatcgac attatcaatc tgcttagata atcttccaga taaaatctcc actgaacgca
     4981 agtaagtaaa gggaatcaga tgtgcaatcc tttgaagagg ctgaattgtt tggatgccaa
     5041 acaataagcc aacaatccca atgagtgaaa gaaagaggac aggcattttt tgcttgaaaa
     5101 agtaagcaat caagtacaca acttccacaa tgacgataaa ggctaagaaa gctaagagca
     5161 agccaggaaa taatacatct tgtattttcc caatagttac ttcttgattc actaagctat
     5221 aaattgggta gggataatct aactgtccaa aaccacttat cagacttccc actagaaaag
     5281 aaaagccaca gattccgata aacagcacag ttacatatcc cactccaact ccaagagagg
     5341 atattgcaaa tgtcactttt gaaacaggat ataagtgagc tgtgtccaga tgattttgat
     5401 atctttctgc aaatagttgt gttagcataa aaataatcgc aaccacaaac aagcttggga
     5461 tgataacctc taaaatccag acaatctgat caatcccgtg ggtcggaaac tccaaagtat
     5521 gtgcttttat gttcaaggga tacagggctt ggtaaatctt ccgttcgcgg tcaaccccca
     5581 tttttaagcc agagctagca gtcgggtcat ttgatacaaa ttcataattc ttctcttcat
     5641 cttgccactg cagatagtag gcttctttcc agcgcccttc ttttagtaaa gttagaattt
     5701 ctgtctttcg cgtcaaaaga tttttttgca cgtctaaatt atttttagca aactggtatt
     5761 cctccgagct ggtatcagac atttgggaga gtttctcttc attttcattg atagccctct
     5821 cgttggctgc aatgcgactt tccaacctgc tctccaagct gtgtgagttt gcagtctgac
     5881 tatttaaata aaaggtaaca ccgagtacag atgcaaataa aagtaagata atccagttta
     5941 aacgactttt gaaaactttc tttaacaaaa atagactaac atctttcata aactaaacct
     6001 cttctatctg cccctgatga atggttacta ctctatcgca gacatcaacc aactcttcct
     6061 tatagtggga acttaaaaga accagctgtt cttgtctatc gatttgtgct agcctatcaa
     6121 aaaacttctg tcgataatac tcatctaagc catttgtaat ctcatccatg agccagcatt
     6181 tggcctgact gaggaaatac atggcaatca ccaagcgttg cttcatgcct aaggaatact
     6241 tgcgaatggg aagactgata tagtcagaca tttcccaata ggcgatttca tccctcaagt
     6301 tcagacctga cttccagatg tttttgataa gacgaaggta gtccatccca cttaagtttc
     6361 catccagcca ttcaatactc tcataataaa ataaagaagg aggggctgcg atatttccac
     6421 tacttatggg aattaaattg ctaatggcac ggaacagggt cgtctttcca gagccattga
     6481 tagcaagaag accataaacc ctaccctttt taaaggtgaa atccacatct tgcaagatga
     6541 cttgtcgcgt ttttaaggtc acatgagtaa gctgcaacat atctagccct cctttttctc
     6601 actctttaag aattaatagc ctccgctgta atagtttatg acctcataac gaccgtagtt
     6661 ccagcctccg ccaactttat actcagaata gctgtcataa cgagaccatg acggaatcca
     6721 agcatactga tggtcgtgat agttggtact atattcccaa accgtattcc aatcatactt
     6781 gtaactttta gtgactgtca cagcagatac actggactga agaagaccaa tagattataa
     6841 actaactaat taaacgattt ttgctaattt ttaatgattt tatatcctca atattgaata
     6901 cgttttcatt ataccatttt tatcattatt atgcaacaaa tttttagttt atttcactaa
     6961 tctacaacat ttatccttcc tatcgaacac tttcgatttt ccaactatcc acccccttaa
     7021 aacgtatagc tccttgctgg tcagttcgat aaactttgct attgatacct tccagtcgtg
     7081 tcaatgtttc ctgatggggg agtttcattc gattgctctt tccaactgag ataagagtaa
     7141 gctctggttt gagtttttct agaaaggctg gacttgatga ttttttattt ccttgttggc
     7201 tagctttcaa aacatttact ttcaagtctg gatagtgctt cagcaagtcc ttctctcctt
     7261 tctcctccaa atttcccgtg aagagaaatt gcttatccaa gaatttccca tacagaacta
     7321 gggtatcatc gtgtcctcca tctcccattt tccttggaga tagaacttct aactgacttc
     7381 caaaaatggg caagttctcc cctactgtca tactacgcac ctttgtttga gtcgcctgta
     7441 gttctgccac aaattccttc tgtttcagac tgtcttttga tactagaatc tcccctacat
     7501 ggaaagcctt ggtcacctct gacaaatctc caacattctc cttgtccgtg ttagtcaaaa
     7561 ttagctggtc aatcttagct actcctcgac ttttgagata gggaatcaag gttcgctggg
     7621 cattgctggt cgtcatcttt tcttgccatt tttcgatttt cttataagat tctgccttac
     7681 cacctacatc tatgagaatg gttttcccag ttacatcccg taggaaaata ctttctcctt
     7741 gccccacatc cagcatggtg atttcatttt ccagtggata cttggtaagg aaaaagagac
     7801 ctgtaatcaa taaactcaat actgttaatc ctttaatgtt tttcctcaaa tcatagacca
     7861 aagccaagga aattaacaat aagattaaaa gccatgcgtt gggttgacca aagacaagcg
     7921 gtctccttgc cacctgcgag accaagcgaa taatgccctc taaccattca aagataaagt
     7981 tcagctgaat gactggatag agaaaggaaa ggacaaataa gatagacaag agcggtaaga
     8041 agaccaagtc aaaaagaaag gaaaagacaa aggtcaaaag gatagaccaa ggttgaaatt
     8101 ccgcaaaata gaaggataga atgggcaata tgcccaagga gatgactaga ctttcactag
     8161 taacagcctt gagcccctcc ccttctttgc tggtcatggt caggataaaa gcataagcac
     8221 aggacaagac tcctcctgct gtcaagaaaa agtttggcat gacaataaag aggacaagca
     8281 ccgtcaaggc aaaattatcc aagcccttaa ccccatgttg agccagtagc ttttgcaaga
     8341 gactgcgaat aaccgatgct gaaaatccag ttagtcccgc atagataagg gaaaagggat
     8401 aagtcagcca cttcaacttt tcttgtgtca agcccaatcg taaaagtagt ttcttaaatc
     8461 cgtccatgaa aaaccctacc tgcatacctg acaaggcaaa aaggtgaata attcctaaac
     8521 tggaataaag ctcattcatc tcctcaaaat cggtgtccag atgtcctagc aagagtcctg
     8581 tcatgtaatt gcgcataggg tctgggaagt gcatcttaat ccaaaccaca gcctttcgac
     8641 gtaaactgga caggttttca cctatatccc aactgccaac cttttggaat gactggattc
     8701 ttttaatatt gagtgtctgg taaattccct gagtcttcag atagccttgg tagtcaaagc
     8761 caccaaaatt tctctgccct tctggctccg aaagcttccc ttctagtcct atctcatgaa
     8821 ggtcagttaa agcttgaaag gcttctttct cctcctcgga ctggagttta taatagactt
     8881 ggaaagcacg accatcagac ttaccacgaa aggacagact gtcaccgtta accttaatag
     8941 tgtctggtaa aatccgtacc ctttcaacag aatccaccaa attttgactc acttgactct
     9001 gttgccaagt ttgaaacaga aaccagaagc caaagactcc acaaatcact agaactttac
     9061 ctgctgattt ccaaggaaat tggataaaga ggcagactag cagaaaaaca aaacccaaca
     9121 aagcaaaata ggatactgag aaaatggcgt agtaaagcca tagtaacaga aaactcaggt
     9181 aaattagggg aatagagaaa ttcttaatcc actgtaacat agtctttaag tttttctatg
     9241 gtcttggcac caatgccaga gaccttcttg agctcgtcta ctgacttgaa cttgccattt
     9301 gcctcacgat ggtcaataat gtcctgagct cgttttcctc ccagtccctt gacctgcttg
     9361 agttcttcca gactggcctt gttgagattg accttctttt ccttgcttgt tgaagaagct
     9421 gtccccgaac cagtctgttg actaactgct tcttctccct tagtaggaac gtaaaccaga
     9481 gcctcatcac taactttctg agctagattg agcgacttgc tgtctgcttg ctctgtcaag
     9541 ccaccagcct tctgaacagc atcattgact cgactaccta caggcaagtc ataaatccct
     9601 ggcgatttga cagcaccttt gacatctact gtgattagat cttgttcaac tggttcttcc
     9661 ttctcttcct tgttcacttc ctttccggtc gatgagtcct tggaaacagc tgcaacttca
     9721 gcctgcaaat tcgtctcttt gacagatgtt tgtggagctg gctttagcag gaaaaatccg
     9781 cctacaagca agcccagacc agtacagatg acgatgattt tatactcttt gattttctcg
     9841 ataattgctt ccatattttc tcctctctta gattattcgt aagaggaaga aaaaacagtc
     9901 gaaaaatttc ttttcaactg cttatttatt tgcaaaatag tcttccttgg tcaagacata
     9961 atgaactctt gtcacgattc ggcctttttc atgctggtcc atacaagcat atggttctgc
    10021 atgggaaaaa cgcatgcctg atttctccat gacctttcct gacgcgggat tagccttatc
    10081 gtgaagggca gtcaacttat tcatccctat cttctcaaaa gctagctcaa tcacagcacg
    10141 attggcttct gtcgttaatc cttgattcca atacttttta ttgataatgt agccaatagc
    10201 tgccttctta agaacaggat caatcttgtg taagtcaata gttccgataa acttgccgtt
    10261 atcttttaat tcaatccccc aacgtcccaa gggattagcc aagtagaact gagcaatgtt
    10321 attcttggtt tcttccaagc tttgattggt tggaaaagtg taacgtgtat tacccttgtc
    10381 cgaggcatag tcaaacattg cttccgcatc atccaaagtt acaggtctaa gcaataaacg
    10441 ctccgtttct atagacggat actgggcaaa tttcacaaat attgattcca tacttttccc
    10501 tccattagag cttgattttc actagtctag cataaaaaaa tttggatgac aagtttttct
    10561 tgatttttga agcggtttca tatacaatga aaccgtcaca tttttgattt atgaactgga
    10621 atgtgacaac caaaacaaga ccgtcaattg aaaggtaggt tatctcaatg ttaatcggaa
    10681 tcccaaaaga aattaaaaat aacgaaaacc gtgtcgccct cacacctgca ggtgttcata
    10741 gcttagttag tcgtggtcat cgtgtcctta tcgaaacaaa tgctggtctc ggttctggct
    10801 ttactgatgc tgactatcaa aagcaaggag ctgagattgt cgctactgct ggtgaagctt
    10861 gggcagcaga gttggttgtg aaagtaaaag aacctttaag ttctgaatac ggttacttgc
    10921 gcgacgatct tcttctcttc acctacttgc acatggccgc tgctccagaa ttagcagatg
    10981 ctatgttagc agcaaaaaca acaggaattg cctatgaaac tgttcgtgac aatcaaggac
    11041 aactaccgct cctcgttcct atgagtgagg ttgcaggtcg tatggctgtt caaatcggag
    11101 ctcacttcct tactaagcaa gctggtggct ctggtgttct acttggtggt gtaccaggtg
    11161 ttccaaaagg aaaagtaact atcatcggtg atggtgtcgt cggtacacat gctgcccgca
    11221 tcgcccttgg tcttggtgct caagtgacta ttttagatat tagtgccaag cgtctctcag
    11281 ttctagaaga agtctttgga agtcaaattc aaactattat atctaattca ttcaacattg
    11341 aagcaagtgt gagagatgct gatgtggtga ttggagccat tctcatccct ggtgcaaaag
    11401 caccggaatt ggtgacagat gagatggtca aacaaatgcg tccaggctct gtatcgttga
    11461 cgttgctgtt gaccaaggtg gcgttatcga aacagctgac cgtgtgacaa cgcacgatga
    11521 acccgtctat gaaaaacacg gtgttctcca ctatgccgtt gccaatatcc ctggtgcggt
    11581 tgcccgcact tcaaccatcg ccctaaccaa tgtcactctt ccttatatcg aagctttggc
    11641 tggcaaagga ttcgcacaag caatctctga agatgaaggc ttgcgtcaag gtgtgaccac
    11701 ttatcaaggt tacttgacta gcctaccagt tgctcaagga cttaatcgtg actacactga
    11761 tatcaatgat ttagtataaa ttttgaaaag aaaagggtga cttcgcaagt cctacccact
    11821 gtgctttgtg tcatcagcta ttacatcgtt ggtttattgt actgtatact ttgcaactgg
    11881 agactttcgt tccagactca attgtgcata agaaactcta aattgaataa agtcaacaga
    11941 gtttcgccca accacttcgt cttgtaatga caattatata aaccaaagga agctgagctc
    12001 tttagtccca gcttcttttt atatataaaa ttttacccgt gcaaagacag ggccttagca
    12061 gacttctttt ttacttcgtt cacccttgct ttttcttttt aggtttgggc gttggcagtt
    12121 ggttatacat agctaaaatc aggtcttata gaaacatctt attatcaagt tcttccactc
    12181 aaatcatttc tttggcacct ttgtatggaa actcaaaaga agattggtca atcttatcta
    12241 agactgcttg cacgggttta actaaaaagc gatcgtcata aatgccgcca ataatcttgc
    12301 cgcggaagta aagaatatac tccccaatca tggaacggta agtcacatca tctaatcctg
    12361 ataattgttc caaaacaaat tccaaatagt tcttacttga tgccatttct aatcttctag
    12421 gctctgttca gcgataacaa ccgtatagag ttcttgctta acctcgcatc caattgattt
    12481 aaagccctgc ttttcccaaa aatgctgaga ttgcggattt cccttaacat aagccaaacg
    12541 tgcctttcga aagttcttag caaaataagc tagtgcttct gtcacaatat gactaccaat
    12601 ccctttcctc tgataggctt gatcaaccat aaacaaacca ataaaaacag tctcctcatc
    12661 aggatatgca tagacaaaat ccataacagc cacaaggtca gatccattcc aaaatccaac
    12721 aaaaaactta tcagccttag ctttaccttc aggtagacaa agcatgtcct cttttacagt
    12781 tgcaaaattt ggctctggtg gacaatgctg aaaatacaga ggattacttt catataaaga
    12841 taaaatactt ggaatatcct tttcagttag tatcctacaa ctgtaatact tagatagttg
    12901 gtcaatcatc ttttcaaatt cctactttct tgcgccctgt gattatgaca caggaagatg
    12961 cactgatcgt catcagccac ataaaagttc tttccatcgt gcctaatcgt tgtctcaaac
    13021 ctttagccta tacaactgga ttttcctctc tcaaaagtat attcttttgc aggcgaactt
    13081 cctcaaaatc agtcgtgtgc aacttcagta gaatattcat aggctcggat aatctgagcg
    13141 acaactggat ggcgaaccac atccttggct gaaaaatgaa caaagtcaat ctgatggatg
    13201 ttcttgagtt tatcttgagc atcaatcaaa ccggacttga cattacgtgg caggtcaatc
    13261 tgactgatat ctccattgac aatcatctta gaatgaaagc ctaaacgcgt caagaacatc
    13321 ttcatctgca tgatggtcgt gttttgcgcc tcatcgagaa tgacaaaggc atcatccaag
    13381 gtccggccac gcatataggc aaggggcgca atttcgataa tttcacgctc catgagacga
    13441 gtcgtttggt ctttcccaag aatttgatac aaggcatcgt aaacaggacg aaggtaagga
    13501 tccaccttct ccttaagatc acccggaaga aatccaagac tctctcccgc ttccaccgct
    13561 ggacgagtta ggataattcg cttgacttgc ccacgtttaa gggcagtcac tgccaaggtc
    13621 actgcaagga aggtcttccc tgtacctgct ggcccaattc caaaggtcac atcatgctgt
    13681 ttgacactgt ccacataaag tttttgacct aaggttttga cgcggatagg cttcccagta
    13741 ttatccttga tgatttcttc ttcgtaaagg gcgacaaact tgtcaatttc atcatttttg
    13801 accatactaa tagccgtcac tacatctggc gtaccaacgg tcatcccacg atttaccaag
    13861 gccatgagag cttgaataac ctgacgggct tcctcgcaag cagactcttc tcccaaaacc
    13921 tggacaatct ccgtacgagc atgaatcaca acatcaagct cttcttccat caaacgaaga
    13981 tggcgttcat tggaaccaaa aagatgaaac aggtcatctg gatgactcag ttgaatgtct
    14041 attgaatgtt ccttcaaata aagaacctct ctaatccttt tatttctttt tattatagca
    14101 gagggatgga gaaattacta gcccaaaccc aatgaataaa tgctagtgtt ctagatattc
    14161 ctgccaaatg ctgtcaaaat ctcctaggtt aaaagagaaa ctggcatgct cctccaaaaa
    14221 gcgactgacc tcatcaaaat catctgtatg ttttggaaaa gctgcctctt caaaagcgag
    14281 gtctgccaaa attgctttgg gattgttact tttaggattg cgctcggtca tgagccaagt
    14341 gtaaaatgat tttctcataa gcctccctaa atcaactctg taccaaactg gtcctgcttg
    14401 attttaccag ccttcataag accacctaaa gctttcttga actgaccttt agaaatgcca
    14461 aaggttgcct tgatgtcgtc tggagatgac ttgtcattta aggtcatgaa accgccattg
    14521 ctttccaaat aagtcaaaat catctgagca tcgttttcca acatttcaaa ggagcgtggt
    14581 ttgagggaga ggttcaaagt gcggtccact tcacggaaac caataacgcg cgcatctaat
    14641 acttgcccca aacgtggctc tgcgtaacgc tcgctaggat gaataaaacc aagcatatta
    14701 ttttctggta ggtaaacaaa agttcctgat agcttgagac ggtaaacaat ggctggccag
    14761 ttttggttct gcatgttgtt gtaggcagga cgagcaagac gttggaagtc ttcttgataa
    14821 gccaagagac cccagatacg gtctttctta tccacttcaa gacggatgta gagttggtcg
    14881 cccttcttag gccagagttc cttgagctca gggagaatat cgagtgacac aacgatttcc
    14941 ttgtcaggaa ggcctgtatc cacaaagaca cccaagtcct tacgaacctc tgtgacacgt
    15001 ccccaaccaa attggtcctg agtggcagtc acttctaagc ttgtcaggcg gagtttttgc
    15061 ttcatatccg tgtatgcaaa acctttgacc gtatccccta ctgtatgttg gccttcttcc
    15121 ttagcaagag cataggtttg accatccttt tgcacaaagt aaaaacggtc gttttcatcg
    15181 atgatcagtc caacgataaa acttgcaaga tttgtattca tatttccttc tttcgaataa
    15241 aactcagcca gcaatgccaa ctgagttttt ctgtttattt ttagacttcc aaaagttctt
    15301 tctctttgtt ggcagtcata tcgtcgatgt gtttaacagc atcgtctgtt actttttgaa
    15361 tgtctttttc aagagtcttc aattcgtctt cagtgatttc ttttgctttt tcttgtttct
    15421 tagcttcgtc catagcatcg cgacggatat tgcggacagc cactttagca ttttcgccga
    15481 ccttcttcac ttctttagca aggtcacgac gagtctcttc tgtaagagct gggacaacca
    15541 agcgaatcac agaaccgtca ttagctggtg tgataccaag atcagaagcg ttcaaggcac
    15601 gttcgatgtc tttcaatgaa gacttgtcaa atggtgttac caacaaaaca cgcgcttctg
    15661 gaatcgtaat tgaagcgatt tggttaagag gagtttcaac tccatagtat tctacatgta
    15721 tacggtcaag caagcttgca ttggcacgac cagcacggat accaccaaat tcacgagcaa
    15781 gtgattggtg agactgggtc attctctctt tagctttttc aataattgcg ttagccatat
    15841 tctttcttat tccttttctt cgatattatt tgaaactgtt gttccgatat tttcaccaaa
    15901 tacgacacgt ttgatgttgc ctgattggtt catgttgaag acaaccaagt caatgtcgtt
    15961 gtccattgag agggttgaag ctgttgagtc catgatacga agacctttat tgataacgtc
    16021 acggtgggtc aattcttcaa acttaacagc tgtcttatct ttcttaggat cggcattgta
    16081 aacaccatcg acaccatttt tagccatgag gatggcatcc gcttcgattt cagctgcacg
    16141 aagggccgct gttgtatctg tcgagaagta tggtgaacca attccagcac caaagataac
    16201 gatacggcct ttttcaaggt gacgaagggc acgtccacgg acataaggct ctgccacttg
    16261 ttgcatagca atagctgttt gtacacgcgt atcaacccca acttgttgca atgaatctgc
    16321 catcacaaga gcattcataa cagtcccaag cattccagtg taatctgcct gaacacggtc
    16381 catacctgct tctgctgcag gttctccacg ccagagattt cctccaccaa taacaagggc
    16441 aatttcgata cctaagctat gaacttcttg aatctctttt gcgattgttt gaactgtttg
    16501 gatatcaatc cctacgccac gttcaccggc aagggcttca cctgataact tgattaaaat
    16561 acgtttatac ttgggattcg ccattttcac tctccttctt tcatcctacc tattttatca
    16621 caatttctaa gatttttata gtatcatgaa caattctttc aaaaaaatta gacagtcaaa
    16681 aattcctcta agtcggcaag ggcacgctct gcaatttttt cataacgagc cttcttatca
    16741 cggatacgct cgccttccaa ctccttgatg atcccaaaat tgacattcat tggttggaaa
    16801 tgtttgctgt cggcatgggt aatgtaatga gctaagcttc caatcgctgt cgtctcgggg
    16861 aaaataacct cgctttcttc cttgaagaga cgagctgcgt taattcccgc aactaagccc
    16921 gaagccgccg actcaacata gccttccacc cccgtcatct gaccagcaaa gaagagattt
    16981 ggttgtttct tggaacggta agtctgctca agaagatttg gtgaatccat gtaagaattg
    17041 cgatgcatga caccataacg gacaaactcc gcattttcaa gacccggaat catttggaag
    17101 acacgctttt gttctcccca tttgaggtgg gtctggaaac caacaatatt gtataggcta
    17161 ccagctgcat tatcctgacg aagttgcaca accgcataag gtgttttaaa ttctccatca
    17221 cgaggtcctg tgtagtcgtc cggatactca agaccgactg gcttcatagg gccataaagc
    17281 atagttttaa tgccacgttt ggccataact tcgattggca tacatccttc aaagtacttt
    17341 tctttttcaa aagaattgag cggtgcttct tccgcattga ccaaggcttc atggaaatcc
    17401 ataaactctt gcttggtcat tggagcattg agataggctg cttctccctt atcataacga
    17461 gatttgagat agaccttact catatcgata gtgttgacat cgataatagg cgctgctgca
    17521 tcgtagaaat agaagccatc gccattatta agggcatgaa tcttttcagc cagggcatcg
    17581 ctagtcaagg gaccagtcgc cacaactgta ataacatctg ttggcaattc tgtaatttca
    17641 tcacgcacca cttcaatcaa ggggtggttg accacttttt cggtcaccat ttgagagaaa
    17701 ccatcacgat ccaccgcaag ggctccaccg gcaggaacac gtgtagcctc agcagattcc
    17761 aagataacag aacccaagcg acgcatttct tccttgagaa gaccaactgc atttgtcaaa
    17821 gcatccccac gcaaagaatt ggaacaaacc aactcagcaa aattgtctgt tttatgctgg
    17881 ggtgtagact tgacaccacg catttcatat agtttaactg gaataccacg ctctgcgatt
    17941 tggtaagctg cttcagaacc tgccaaacca gcaccgataa cattgatata agattgagac
    18001 acgacactaa tacctctttg ggagtgtgaa gttaagattc acattgaaaa agccaatcag
    18061 acttacaagc tttcgagttt cttggctcag gctgaaaaag tccattggac ttacttcgct
    18121 ctcgaatttc ttggctca
//
LOCUS       DAVBNO010000038        18101 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVBNO010000038 DAVBNO010000000
VERSION     DAVBNO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18101
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.038"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            3..308
                     /locus_tag="VQ846_001447"
     CDS             3..308
                     /locus_tag="VQ846_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET3076014.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            356..1831
                     /locus_tag="VQ846_001448"
     CDS             356..1831
                     /locus_tag="VQ846_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET3076015.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            1854..1931
                     /locus_tag="VQ846_001449"
                     /pseudo
     CDS             1854..1931
                     /locus_tag="VQ846_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2091..8792
                     /locus_tag="VQ846_001450"
     CDS             2091..8792
                     /locus_tag="VQ846_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET3076016.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKLEEKAPRIESQASSQEKPLKEDAKAVTNEEMNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIANTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLVTVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLVTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPAVPTV
                     TVGGEAVTGLTSQKPMQYRTLAYGAKLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVDLTIQANTEKKIAQSIRP
                     VNVVTDLHQEPTLPSTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARARVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKSLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEITVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKDIKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSASSQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9040..9896)
                     /locus_tag="VQ846_001451"
                     /pseudo
     CDS             complement(9040..9896)
                     /locus_tag="VQ846_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10107..10466)
                     /locus_tag="VQ846_001452"
     CDS             complement(10107..10466)
                     /locus_tag="VQ846_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET3076017.1"
                     /translation="MGMILMKLASILLLILTLVICIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLTLSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYVEMIVELFLMK"
     gene            10558..11514
                     /locus_tag="VQ846_001453"
                     /pseudo
     CDS             10558..11514
                     /locus_tag="VQ846_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11498..12055
                     /locus_tag="VQ846_001454"
     CDS             11498..12055
                     /locus_tag="VQ846_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET3076018.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLRQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12048..12302
                     /locus_tag="VQ846_001455"
     CDS             12048..12302
                     /locus_tag="VQ846_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076019.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12314..14368
                     /locus_tag="VQ846_001456"
     CDS             12314..14368
                     /locus_tag="VQ846_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET3076020.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEKSKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14547..15407
                     /locus_tag="VQ846_001457"
     CDS             14547..15407
                     /locus_tag="VQ846_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET3076021.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNISNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            15626..16336
                     /gene="ccdA2"
                     /locus_tag="VQ846_001458"
     CDS             15626..16336
                     /gene="ccdA2"
                     /locus_tag="VQ846_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET3076022.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16356..16922
                     /locus_tag="VQ846_001459"
     CDS             16356..16922
                     /locus_tag="VQ846_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET3076023.1"
                     /translation="MKKWQTCVLGAGSLLWLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            16933..18045
                     /gene="msrB"
                     /locus_tag="VQ846_001460"
     CDS             16933..18045
                     /gene="msrB"
                     /locus_tag="VQ846_001460"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET3076024.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         5445 a         3449 c         3979 g         5228 t
ORIGIN      
        1 aaatgattaa aattcttgct gcctgcggtg caggtgttaa ctcaagtcac caaattaaaa
       61 gtgctctaga agaagaactt tcaaaccgtg gttatgatgt tcactgtgat gcagtcatgg
      121 tgaaagatgt aaacgaagac cttatgaaag gttacgatat ctttacacca atcgctgcaa
      181 cagaccttgg ttttgaacca ggtatcccag ttatcgaagc tgggccaatc ttattccgta
      241 tcccagcaat gagcgctcca gtatttgaca atattgaagc agctattaaa gaacacggat
      301 taagctaatt ctaatttgtt aacattcata taacataaaa aagggaggaa ctgttatgga
      361 tgcaatcttt gacctaatcg gaaaggtttt caatcccatc ttagaaatgg gtggacctgt
      421 catcatgttg atcattttga cagtattggc tttacttttt ggagtgaaat tctccaaagc
      481 gcttgaaggt ggtatcaaac ttgccatcgc tcttacaggt atcggtgcta tcatcggtat
      541 gctaaatggt gctttctcag catcacttgc aaaattcgtt gaaaacactg gtatccaatt
      601 gagtattaca gacgttggtt gggcaccact cgcaacaatc acttggggtt ctgcttggac
      661 actatacttc ttgctcatca tgttgattgt caacgtagtg atgcttgcta tgaagaaaac
      721 cgatacactt gatgtcgata tatttgatat ctggcacttg tctatcacag gtctcttgat
      781 taaatggtat gctgataaca atggtgtgag tcaaggggtt tcactcttta ttgctacagc
      841 agctatcgtc cttgtcggtg tgttgaaaat tatcaactct gacttgatga aacctacatt
      901 tgatgacctt cttaacgccc caagttcatc accaatgaca tcaactcaca tgaactacat
      961 gatgaaccca gttatcatgg ttttggataa gatttttgaa aaattcttcc caggccttga
     1021 taaatatgac tttgatgctg ctaaattgaa caagaaaatc ggtttctggg gatctaaatt
     1081 cttcatcggt ttcatccttg gtatcgttat cggtattatg ggaactccac atccaattgc
     1141 aggtgttgca gatgcagata aatggcgtct tgttatcaaa ggatggttgt ctcttggttt
     1201 gactgccggt gtatctttgg aactcttctc acttatcggt tcatggttca tcgcagccgt
     1261 agaaccacta tcacaaggta ttacaaacgt tgctactaaa cgtcttcaag gacgtaaatt
     1321 caatatcggt cttgactggc cattcatcgc tggtcgtgct gaaatctggg cttgtgccaa
     1381 cgtacttgca ccaatcatgt tgattgaagc agtgcttctt tcaaaagttg gaaatggtat
     1441 cttgccactt gcaggtatca ttgctatggg tgttactcca gctctcttgg ttgtaactcg
     1501 tggtaaattg ctccgtatga ttatcttcgg aacactcttg ttgccactct tccttctttc
     1561 aggtaccctt attgcaccat ttgcaacaga acttgctaaa ggtgtaggtg ccttcccaga
     1621 aggtgtgagc caaactcaat tgattactca ctctactctt gaaggaccaa tcgaaaaact
     1681 tcttggttgg acaattggta acactacaac tggtgatatc aaagcaatcc ttggtgcagt
     1741 agccttcctt gtattctata tcggtatctt tgcttggtac agaaaacaaa tgatcaaacg
     1801 taacgaagag tacgcagcaa aagcaaaata atgcgctcct ataaaatata aactcaatga
     1861 aaatcaaaga gcaaactagg aaactagcca caggttgctc aaaacacagt tttgaggttg
     1921 tagatgaaac tgacgaagtc agtaatcata tctacggcaa ggcgacgctg acgaagtttg
     1981 aattggattt tcgcagagtg taaattgttg aaactaggtt gtcataccat gtataccact
     2041 tgggctttgg tagtagctaa ctgcactaaa tataatataa ggagggtttt atggggaaag
     2101 gccattggaa tcggaaaaga gtttatagca ttcgtaagtt tgctgtggga gcttgctcag
     2161 taatgattgg gacttgtgca gttctattag gaggaaatat agctggagaa tctgtagttt
     2221 atgcggatga aacacttatt actcatactg ctgagaaacc taaagaggaa aaaatgatag
     2281 tagaagaaaa ggctgataaa gctttggaaa ctaaaaatgt agttgaaagg acagaacaaa
     2341 gtgaacctag ttcaactgag gctattgcat ctgagaagaa agaagatgaa gccgtaactc
     2401 caaaagagga aaaagtgtct gctaaactgg aagaaaaagc tccaaggata gaatcacaag
     2461 cttcaagtca agaaaaaccg ctcaaggaag atgctaaagc tgtaacaaat gaagaaatga
     2521 atcaaatgat tgaagacagg aaagtggatt ttaatcaaaa ttggtacttt aaactcaatg
     2581 caaattctaa ggaagccatt aaacctgatg tagacgtatc tacgtggaaa aaattagatt
     2641 taccgtatga ctggagtatc tttaacgatt tcgatcatga atctcctgca caaaatgaag
     2701 gtggacagct caacggtggg gaagcttggt atcgcaagac tttcaaacta gatgaaaaag
     2761 acctcaagaa aaatgttcgc cttacttttg atggcgtcta catggattct caagtttatg
     2821 tcaatggtca gttagtgggg cattatccaa atggttataa ccagttctca tatgatatca
     2881 ccaaatacct tcacaaagat ggtcgtgaga atgtgattgc tgtccatgca gtcaacaaac
     2941 agccaagtag ccgttggtat tcaggaagtg gtatctatcg tgatgtgact ttacaagtga
     3001 cagataaggt gcatgttgag aaaaatggga caactatttt aacaccaaaa cttgaagaac
     3061 aacaacatgg caaggttgaa actcatgtga ccagcaaaat cgccaatacg gacgacaaag
     3121 accatgaact tgtagccgaa tatcaaatcg ttgaacgagg tggtcatgct gtaacaggct
     3181 tagttcgtac agcgagtcgt accttaaaag cacatgaatc aacaagccta gatgcgattt
     3241 tagaagttga aagaccaaaa ctctggaccg ttttaaatga caaacctgcc ttgtacgaat
     3301 tgattacgcg tgtttaccgt gacggtcaat tggttgatgc taagaaggat ttgtttggtt
     3361 accgttacta tcactggact ccaaatgaag gtttctcttt gaatggtgaa cgtattaaat
     3421 tccatggagt atccttgcac cacgaccatg gggcgcttgg agcagaagaa aactataaag
     3481 cagaatatcg ccgtctcaaa caaatgaagg agatgggagt taactccatc cgtacaaccc
     3541 acaaccctgc tagtgagcaa accttgcaaa tcgcagcaga actaggttta ctcgttcagg
     3601 aagaggcttt tgatacttgg tatggtggca agaaacctta tgactatgga cgtttctttg
     3661 aaaaagatgc cactcaccca gaagctcgaa aaggtgaaaa atggtctgat tttgacctac
     3721 gtaccatggt cgaaagaggc aaaaacaacc ctgctatctt catgtggtca attggtaatg
     3781 aaataggtga agctaatggt gatgcccact ctttagtaac tgttaaacgt ttggtcaagg
     3841 ttatcaagga tgttgataag actcgctatg ttaccatggg agcagataaa ttccgtttcg
     3901 gtaatggtag cggagggcat gagaaaattg ctgatgaact cgatgctgtt ggatttaact
     3961 attctgaaga taattacaaa gcccttagag ctaagcatcc aaaatggttg atttatggat
     4021 cagaaacatc ttcagctacc cgtacacgtg gaagttacta tcgccctgaa cgtgaattga
     4081 aacatagcaa tggacctgag cgtaattatg aacagtctga ctatgggaat gatcgtgtgg
     4141 gttgggggaa aacagcaacc gcttcatgga cttttgaccg tgacaacgct ggctatgctg
     4201 gacagtttat ctggacaggt acggactata ttggtgaacc tacaccatgg cacaaccaaa
     4261 atcaaactcc tgttaagagc tcttactttg gtatcgtaga tacagccggc attccaaaac
     4321 atgacttcta tctctaccaa agccaatggg tttctgttaa gaagaaaccg atggtacacc
     4381 ttcttcctca ctggaactgg gaaaacaaag aattagcatc caaagtagct gactcagaag
     4441 gtaagattcc agttcgtgct tattcgaatg cttctagtgt agaattgttc ttgaatggaa
     4501 aatctcttgg tcttaagact ttcaataaaa aacaaaccag cgatgggcgg acttaccaag
     4561 aaggtgcaaa tgctaatgaa ctttatcttg aatggaaagt tgcctatcaa ccaggtacct
     4621 tggaagcaat tgctcgtgat gaatctggca aggaaattgc tcgagataag attacgactg
     4681 ctggtaagcc agcggcagtt cgtcttatta aggaagacca tgcgattgca gcagatggaa
     4741 aagacttgac ttacatctac tatgaaattg ttgacagcca ggggaatgtg gttccaactg
     4801 ctaataatct ggttcgcttc caattgcatg gtcaaggtca actggtcggt gtagataacg
     4861 gagaacaagc cagccgtgaa cgctataagg cgcaagcaga tggttcttgg attcgtaaag
     4921 catttaatgg taaaggtgtt gccattgtca aatcaactga acaagcaggg aaattcaccc
     4981 ttactgccca ctctgatctc ttgaaatcga accaagtcac tgtctttact ggtaagaaag
     5041 aaggacaaga gaagactgtt ttggtgacag aagtgccaaa agtacagacc attattggag
     5101 aggcacctga aatgcctacc actgttccgt ttgtatacag tgatggtagc cgtgcagaac
     5161 gtcctgtaac ctggtcttca gtagatgtga gcaagcctgg tattgtaacg gtgaaaggta
     5221 tggctgacgg acgagaagta gaagctcgtg tagaagtgat tgctcttaaa tcagagctac
     5281 cagttgtgaa acgtattgct ccaaatactg acttgaattc tgtagacaaa tctgtttcct
     5341 atgttttgac tgatggaagt gtacaagagt atgaagtgga caagtgggag attgccgaag
     5401 aagataaagc taagttagca attccaggtt ctcgtattca agcgaccggt tatttagaag
     5461 gtcaaccaat tcatgcaacc cttgtggtag aagaaggcaa tcctgcggca cctgcagtac
     5521 caactgtaac ggttggtggt gaagctgtca caggtcttac tagtcaaaaa ccaatgcaat
     5581 accgcactct tgcttatgga gctaagttgc cagaagtcac agcaagtgct aaaaatgcag
     5641 ctgttacagt tcttcaagca agcgcagcaa acggcatgcg tgcgagcatc tttattcagc
     5701 ctaaagatgg tggccctctt caaacctatg caattcaatt ccttgaagaa gcgccaaaaa
     5761 ttgctcactt gagcttgcaa gtggaaaaag ctgacagtct caaagaagac caaactgtca
     5821 aattgtcggt tcgagctcac tatcaagatg gaacgcaagc tgtattacca gctgataaag
     5881 taaccttctc tacaagtggt gaaggggaag tcgcaattcg taaaggaatg cttgagttac
     5941 ataagccagg agcagtcact ctcaaagcgg aatatgaggg agctaaaggc caagttgatc
     6001 tcactatcca ggccaatact gagaagaaga ttgcgcaatc tatccgtcca gtaaatgtag
     6061 tgacagattt acaccaagaa cctactcttc cgtcaacagt aacggttgag tatgacaaag
     6121 gtttccctaa agctcacaaa gtcacttggc aagctattcc gaaagaaaaa ctagactcct
     6181 atcaaacctt tgaagtacta ggtaaagttg aaggaattga ccttgaagcg cgtgcaagag
     6241 tctctgtaga aggtatcgtt tcagttgaag aagtcagtgt gacaactcca atcgcagaag
     6301 caccacaatt accagaaagc gttcggacat atgattcaaa tggtcacgtt tcatcagcta
     6361 aggttgcatg ggatgcgatt cgtatagagc aatacgctaa ggaaggtgtc tttacagtta
     6421 atggtcgctt agaaggtact caattaacaa ctaaacttca tgttcgcgta tctgctcaaa
     6481 ctgagcaagg tgcaaatatt tctgaccaat ggaccggttc agaattgcca cttgcctttg
     6541 cttcagactc aaatccaagc gacccagttt caaatgttaa tgacaagctc atttcctaca
     6601 ataaccaacc agccaatcgt tggacaaact ggaatcgtag taatccagaa gcttcagtcg
     6661 gtgttctgtt tggagattca ggtatcttga gcaaacgctc cgttgataat ctaagtgtcg
     6721 gattccacga agaccatgga gttggtgcac cgaagtctta tgtgattgag tattatgttg
     6781 gtaagactgt cccaacagct cctaaaaacc ctagttttgt tggtaatgag gaccatgtct
     6841 ttaatgattc tgccaactgg aaaccagtta ctaatctaaa agcccctgct caactcaagg
     6901 ctggagaaat gaaccacttt agctttgata aagttgaaac ctatgctgtt cgtattcgca
     6961 tggttaaagc agataacaag cgtggaacgt ctatcacaga ggtacaaatc tttgcgaaac
     7021 aagttgcggc agccaaacaa ggacaaacaa gaatccaagt tgacggcaaa gacttagcaa
     7081 acttcaaccc tgatttgaca gactactacc ttgagtctgt agatggaaaa gttacggcgg
     7141 tcacagcaag tgttagcaac aatggtctcg ctaccgtcgt tccaagcgtt cgtgaaggtg
     7201 agccagttcg tgtcatcgcg aaagctgaaa atggcgacat cttaggagaa taccgtctgc
     7261 acttcactaa ggataagagc ttactttctc ataaaccagt tgctgcggtt aaacaagctc
     7321 gcttgctaca agtaggtcaa gcacttgaat tgccgactaa ggttccagtt tacttcacag
     7381 gtaaagacgg ctacgaaaca aaagacctga cagttgaatg ggaagaagtt ccagcggaaa
     7441 atctgacaaa agcaggtcaa tttactgttc gaggccgtgt ccttggtagt aaccttgttg
     7501 ctgagatcac tgtacgagtg acagacaaac ttggtgagac tctttcagat aaccctaact
     7561 atgatgaaaa cagtaaccag gcctttgctt cagcaaccaa tgatattgac aaaaactctc
     7621 atgaccgcgt tgactatctc aatgacggag atcattcaga aaatcgtcgt tggacaaact
     7681 ggtcaccaac accatcttct aatccagaag tatcagcggg tgtgattttc cgtgaaaatg
     7741 gtaagattgt agaacggact gttgcacaag gaaaagttca gttctttgca gatagtggta
     7801 cggatgcacc atctaaactc gttttagaac gctatgtcgg tccagagttt gaagtgccaa
     7861 cctactattc aaactaccaa gcctacgacg cagaccatcc attcaacaat ccagaaaatt
     7921 gggaagctgt tccttatcgt gcggataaag acattgcagc tggtgatgaa atcaacgtaa
     7981 catttaaaga tatcaaagcc aaagctatga gatggcgtat ggagcgtaaa gcagataaga
     8041 gcggtgttgc gatgattgag atgaccttcc ttgcaccaag tgaattgcct caagaaagca
     8101 ctcaatcaaa gattcttgta gatggaaaag aacttgctga tttcgctgaa aatcgtcaag
     8161 actatcaaat tacctataaa ggtcaacggc caaaagtctc agttgaagaa aacaatcaag
     8221 tagcttcaac tgtggtagat agtggagaag atagccttcc agtacttgtt cgcctcgttt
     8281 cagaaagtgg aaaacaagtc aaggaatacc gtatccactt gactaaggaa aaaccagttt
     8341 ctgagaagac agttgctgct gtacaagaag atcttccaaa actcgaattt gttgaaaaag
     8401 atttggccta caagacagtt gagaaaaaag attcaacact gtatctaggt gaaactcgtg
     8461 tagaacaaga aggaaaaact ggtaaagaac gtatctttac agcgattaat cctgatggaa
     8521 gtaaggaaga aaaactccgt gaagtggtag aagctccgac agaccgcatc gtcttggttg
     8581 gaaccaaacc agtagctcaa gaagctaaaa aaccacaagt gtcagaaaaa gcagatacaa
     8641 aaccaattga ttcaagtgaa gctagtcaaa ctaataaagc ccagttacca aatacaggta
     8701 gtgcgtcaag ccaagcagca gtagcagcag gtttagctct tctaggtttg agtgcaggat
     8761 tagtagttac taaaggtaaa aaagaagact agaaattttg ataccttctt tatcattaaa
     8821 gaccaaagaa aaactcttgt cctaaataga agataaagag tctggaacaa aatagttctc
     8881 aaccacaaaa aagctagata tttccaattg cggaactcta gctttttaat tttgagtcgt
     8941 tctctgattg tatagtagat tgaaactaga gtagtacact tctacttcta aaacattgtt
     9001 agaaatcgat ttgactgtcc ttatcgattt gtcctgttct tatttgattt cactatgttt
     9061 taggaggtta ttggccataa gtaccaatcc catgtcaatt ctcacttgac gcttgcctct
     9121 cagattacat ctcttgtaac ccaaacaagc ctttatctgc ccaaagacag gttccacatc
     9181 aatcttgcgt tgagcgaaaa tctgtctatc ttggggagat aaaagcgcct gacattcttt
     9241 agctttcaag ttttgatagc gttcgttcat atacagtccc ttttgagggg ctgattcagg
     9301 ttcatcagcg tagtaaacct tgatttcctg ttgaaagtct gtctgtgttt tctggtactt
     9361 gatatggtga aaacgatagc accagccatc aggatgtatg tagctatcct ccttgtcatt
     9421 atagtgccaa ttcgctaagt ttttagctga ctgtttatac cctctctttt gtttcttatc
     9481 aaacatggca tatttaatca gaaggggagt gatttcttct tgaaaataga cttgtatctg
     9541 ttcttgaagt ttggcggaga atttctctgt cgttttcttc cacacaaaac tatacttgtt
     9601 ggcattggct tcaatcttag ttccgtcaat aaacaaacaa tctaaggtca ctaactcttc
     9661 catttttcaa tttttcgacc agagaaaatc ccttgtgaat aggcaaatag aagagtagat
     9721 acaagcattt taaggtgata agacgggcga tcaaaggcat gatagaaggc gtggaaatga
     9781 caatcctcca aggtattcac cactttttca atagtaaaaa cgagatggtc ttgtggcaag
     9841 aaggaactga tttctagtgg taaagttgtt tgatttgtgt tatagtgaat atgcatggga
     9901 tagcctttct aaatggttta ttgtgcaatt ctattttacc aagactatcg caaccatgca
     9961 aaaaagcaac ggcaaatccg ttgctttttt tggagataag agactattgt cccagactct
    10021 tttctttgta taaaattgta tagtgttatt gtctatacta ttttgtagag aaagcaaaaa
    10081 tccctagaaa atgcttaggg attcgactat ttcattaaga acaattcaac aatcatttct
    10141 acatacatga taagggttaa taagaatcct gcacgccaga agaatttgat gaatttagga
    10201 tagtaaaagc tgcgtttttt caaaaggaag aaaaagacga gaataatggc taggatcgag
    10261 agggttagcc ccagtctagg gaggaaatta tgggtaaagg ttttagctgt aatcaagtaa
    10321 tactcaaata ccaagactgg aaaagccaaa tccgcaaagt ttcgtcctag tttttttaat
    10381 ctaaaaagtt tggttaggat aatgcagatg actaaggtca gtatcaataa taaaatagat
    10441 gctaatttca ttaaaatcat acccatattg tatcataaaa aaaggctaat ggaaacgaga
    10501 aacagaggaa tttataagaa agtcaggctt ttgagattgt gggtgatttt tgttataatg
    10561 aaagttataa aatcttataa taccttgaat gattattatc gaaaactctt tggagaaaag
    10621 acttttaaag tccctattga tgcgggattt gactgtccca atcgtgatgg aactgtggct
    10681 catggaggct gtactttttg tacggtttca ggttctggag atgctattgt agccccagat
    10741 gcgcctatcc gtgagcaatt ttataaggaa attgacttta tacaccgtaa gtggccggat
    10801 gttcagaagt atctggttta ttttcaaaat tttaccaaca ctcatgaaaa ggtggaagtt
    10861 attcgagagc gttatgaaca ggctatcaat gagccaggtg tggtaggaat caatattgga
    10921 acgcgcccag actgtttacc agacgaaacc atcgaatatt tggctgagtt atcggagtgc
    10981 atgcatgtga cgtttgaatt gggcttgcaa actacttatg aagcaacctc tgacctgatt
    11041 aaccgtgcgc attcttatga attgtaggtg gaaacggtca agcgtttgag aaagtatcca
    11101 aaaattgaga ttgtttccca tttgatcaat ggtttgcctg gtgaaaccca tgagatgatg
    11161 gttgaaaatg tccgccgttg tgtcacggat aacgatattc aagggattaa actgcatttg
    11221 ctccatctta tgacaaatac tcgtatgcaa cgagattacc atgaggggcg tttacaattg
    11281 atgagtcagg acgaatatgt cagggtcatc tgtgaccaac tggaaattat tcccaaacat
    11341 atcgtcatcc atcgaattac aggagatgcg cctagggata tgctgcttgg gcctatgtgg
    11401 agcctcaata aatgggaagc tctcaacagc attgagatgg agatgcgacg tcgtggaagt
    11461 gttcaaggat gcaaggctgt aaaacaggag tttgaaaatg aaaagaccac ttgagatggc
    11521 acatgatttt ttggctgagg tcgtgacaaa agaggatgtc gtagtggatg cgactatggg
    11581 aaatggtcat gacacgcttt ttttagccaa gctagccaag caagtctatg cctttgatat
    11641 tcagaagcaa gccttggaaa agacccaaga gcgtttgcgt caggctgact tgacaaatgc
    11701 ccagttaatc ttgcaaggcc atgagacact ggaccagttt gtgataaaag ctaaggcagg
    11761 gatttttaat ctgggctatt tgccgtcagc tgataagtct gtcatcaccc gaccgcagac
    11821 aacgattgag gcattagaaa agctatgtgg cttacttgtc aaaggtggac gaattgctat
    11881 tatgatttac tatggtcatg aaggaggcga tctcgagaga gatgctgtct tggattttgt
    11941 gatccagttg aaccaacaag agtacacagc tgccatttac cgaactttaa accaagtcaa
    12001 caacccgccg tttttagtga tgattgaaaa attagagaga tacagacatg gataaacaat
    12061 acttacacga aaagctggat gccatgcgcc agaattttgt tgaatcaacc caccacgaac
    12121 gagcggtggg cgtgctagac caagcgcata tgagcaaaaa aatgcttaaa atcaagaaaa
    12181 aattagttgc tcttgaaatg gaacggtgcc agagaaaaat tgagcacaaa gactgttcca
    12241 agattgacca aaaaatcaaa gagcagaagg agatatttga atcctgttgt aaaaaagatt
    12301 aaggaggacg tgcgtggaat tactgattta cctcatccta tttttactgg ttttgattgt
    12361 ctcaagtaca accaataagc tcctgccctt tttgcctctc cctttggtgc aaattctttt
    12421 gggaattgtg attggtctct ttttacccaa tactgacttt caccttaata cggagttgtt
    12481 tttggcactg gttatcggac ccttgctttt ccgagaggct gaagaagcag atgttacggc
    12541 tattttaaaa cactggcgaa tcattgttta tctcatattt ccagtgattt ttatctcgac
    12601 cctgagtttg ggtggcttgg cccatcttct ttggttcagc cttcccttgg cagcttgctt
    12661 ggctgttggg gcagcccttg gtcctacgga cttggtggcc tttgcctctc tttcggagcg
    12721 ttttagcttt cctaagcgcg tgtccaatat tcttaagggc gaaggactct tgaatgatgc
    12781 ttctggtttg gtggcttttc aggtagcttt gacagcttgg acaactggag ctttttctct
    12841 ggggcaagct agcagttcgc tcatcttttc aatcctaggc ggttttttaa ttggattttt
    12901 aacagccatg accaaccgct tcctccatac cttcttgcta agtgtgcgcg caacggatat
    12961 tgccagtgaa cttttattag aattgagttt gcctctagtg accttctttc tggcagaaga
    13021 agtccatgtt tcaggtatta ttgccgtcgt agttgctgga attttaaagg caagtcgctt
    13081 caagaaaatc acgctcctcg aagcccaagt ggatacggtg accgagacgg tctggcatac
    13141 agtgaccttt atgctcaacg gttctgtctt tgtgatttta gggatggagt tggaaatgat
    13201 agcagaacct atcttgacca atccaatcta taatcctcta cttttattgc tatctctcat
    13261 cgcccttacc tttgtcctct ttgtcattcg ttttattatg atctatggct attatgccta
    13321 tagaacccga cgcctcaaga aaaagctaaa taagtatatg aaggacatgt ttctcttgac
    13381 cttttcaggt gttaagggaa cggtgtcgat tgctacgatt ctcttgatac caagtaatct
    13441 agaacaggag tatcctcttt tgcttttcct tgttgcaggt gtgacgcttg tcagcttttt
    13501 aacaggtctc ttggtcttgc ctcatctttc tgatgaagag gaaaaaagca aggattatct
    13561 catgcatatc gccattttga atgaagtaac gctagagttg gaaaaagagt tggaagacac
    13621 cagaaataaa cttcccctct atgcggctat tgacaattat catggacgta ttgaaaatct
    13681 cattttaagc caagaaaacc aggatgatca agaagactgg gctgctttga aactcttgat
    13741 tcttagtatt gaaagtgatg gtttggaaca ggcctatgaa gaggggaaca ttagcaatcg
    13801 tgcttaccga gtttaccaac gttatctgaa aaatatagaa caaggaatca atcgtaaact
    13861 tgcctcaaga ctgacctatt atttccttgt ttccttgagg attttacgtt ttctccttca
    13921 tgaagttttt actcttggaa agaccttccg tagctggaag gacaaggagc aaagccgtct
    13981 ccgtgctctt gattatgacc aaattgcaga gctctatctt gccaatacgg agatgattat
    14041 tgaaagtttg gaaaacctga agggagtcta cagacgctct ttgattagtt ttatgcagga
    14101 gtctcgtctt cgagaaacag ctattatcag cagtggtgcc tttgtcgaac gggttatcaa
    14161 tcgtgtcaaa cccaacaata tcgatgaaat gctgagaggc tattatctgg agcgcaagtt
    14221 gattttcgaa tacgaagaaa aacgattaat tacaactaag tatgccaaga aattacgaca
    14281 aaatgtaaat aacttagaga actattcctt gaaggaagct gccaataccc tgccgtatga
    14341 tatggtggaa ttggtaagaa gaaattagtt aatactcttc gaaaatctct tcaaaccacg
    14401 tcagcgtcgc cttggattat atatgtgact gacttcgtca gtttcatcta caacctcaaa
    14461 gcagggcttt gagcaacctg cggctagctt cctagtttgc tctttgattt tcattgagta
    14521 taagattata aatgaaggag tatgacatga aaaagtggtg gaaagagctg atagacaagc
    14581 ctttattaaa agcttttttg cattattatc aagcatcaga tagtgagttg accagtgtcg
    14641 cagtagccta ctattggttg atttcgattt tccccctgct tttggtggtg gtcaatatcc
    14701 tcccctattt tcagattcct gtgggagaat ttctgtgctt tatgaaagac gttctgcccc
    14761 caagcctcta tgaaggtgtg gaaaaaatag ccagagaagt tttgacgcaa ccttcaacag
    14821 gtttattaag tttttctgtt ttatcggcgt tatggagttt ttcaaaatct atgaatttct
    14881 tgcaaaaagc ttttaacaag gcttatggcg tcgaaaagag tcgtggactt atttcccatc
    14941 agatgctaag tctcctagtc agttttggct tgcagctcct ctttgcgttt gccttgtttt
    15001 tgatcttatt tggtcagatg attttggctt tacttgctca ttattggaca aaagatggca
    15061 ttatctatca ggctttacaa gggttggcag gtcctctgat ttacgccctc ctctttgcta
    15121 tcttggtcat gctttattat tttcttccca atatatctaa caggaaaatt agctataccc
    15181 taccaggcag tgcctttgtt ctcttagtga ttttaggttt actaactctg ttctctagtt
    15241 atctcaatta ctatgtccat catttggtgg atgtccgaat tttaggttcc gttcttcttg
    15301 ttgttatgat gttttggttt atcttgattg ctaaaattgt tatcctaggc gcggttatca
    15361 atgccagtat gcagagtttg aaagatccgg tctttaaaaa agactaacta tttatccatt
    15421 ataaaagcgc atagtatcaa gactttatag aaccttgata ctatgcgctt ttgcgctttt
    15481 gtgctttgac agttttttgt atagtttttg tccaagattt ctaaatcatc tgattaaatt
    15541 cctattttcc cctatcattg tccctgtttt ttcgaaaatg gaaggagtat aataaatcta
    15601 tcaataaaat gataggagga actttatggg tcatattttc ttttttctaa gtgtcttttt
    15661 ggcagggatt ctatccttct tttctccttg tatcttacct ttgttaccgg tctatacagg
    15721 agtgttacta gatgataagg atggtgctca ggcttctagc ggcaaatttt caatctcagt
    15781 tactagttta ttacgaactc tggcctttat agcaggaatt tcctttatat ttattttgtt
    15841 gggctatgga gctggttttt taggcgattt gctttatgct tcttggttcc aatatcttac
    15901 tggggcaatt attatccttc ttggtttgca ccaaatggag attctacact ttaaggggct
    15961 ttataaggaa aagaggctac aactgcaagg acaggggcaa aatggtaagg gctatagtca
    16021 ggcattttta ttgggcttga cctttagttt tgcttggacg ccttgcgtgg ggccggttct
    16081 ggggtctgtt ttggccttgg cggcttcagg tggttcagga gcttggcagg gagctggtct
    16141 catgttggtg tatacgctgg gcttggcgct accattcttg cttctagctc tgacctctag
    16201 ttatgttttg aaacatttcc gaaaacttca tccctatctc ggaatcctca aaaaagtggg
    16261 tggttttctc attattgtga tgggactctt ggttctgttt ggaaatgctt caattttaag
    16321 tcaattattt gaataaaatg gaaaggaata tcaatatgaa aaaatggcaa acatgtgttc
    16381 ttggagcagg ttcgctcctt tggttgacgg cttgttcagg caagtccgtg actagtgaac
    16441 accaaacgaa agatgaaatg aagacggagc agacagctag taaaacaagc gcagctaaag
    16501 ggaaagaggt ggctgatttt gaattgatgg gagtagatgg caagacctac cgtttatctg
    16561 attacaaggg caagaaagtc tatctcaaat tctgggcttc ttggtgttcc atctgtctgg
    16621 ctagtcttcc agatacggat gagattgcta aagaagctgg tgatgactat gtggtcttga
    16681 cagtagtgtc accaggacat aagggagagc aatctgaagc ggactttaag aattggtata
    16741 agggattgga ttataaaaat ctcccagtcc tagttgaccc atcaggcaaa cttttggaaa
    16801 cttatggtgt ccgttcttac ccaacccaag cctttataga caaagaaggc aagctggtca
    16861 aaacacatcc aggcttcatg gaaaaagatg caattttgca aactttgaag gaattagcct
    16921 aggaggtgtc ttatgaatga taagttaaaa atcttcttgt tgctaggagt attttttcta
    16981 gccataaccg gtttctatgt tctattgata cgaaatgcag ggcagacaga tgcctcgcaa
    17041 attgaaaagg cggcagttag ccaaggagga aaagcagtga aaaaaacaga aattagtaaa
    17101 gacgcagact tgcacgaaat ttatctagct ggaggttgtt tctggggagt ggaggaatat
    17161 ttctcacgcg ttcccggggt gacggatgcc gtttcaggct atgcaaatgg tagaggagaa
    17221 acaaccaagt acgaattgat taaccaaaca ggtcatgcag aaaccgtcca tgtcacctat
    17281 gatgccaagc aaatttctct caaggaaatc ctgcttcact atttccgcat tatcaatcca
    17341 accagcaaaa ataaacaagg aaatgatgtg gggacccagt accgtactgg tgtttattac
    17401 acagatgaca aggatttgga agtgattaac caagtctttg atgaggtggc taagaaatac
    17461 gatcaacctc tagcagttga aaaggaaaac ttgaagaatt ttgtggtggc tgaggattac
    17521 catcaagact atctcaagaa aaatccaaat ggctactgcc atatcaatgt taatcaggcg
    17581 gcctatcctg tcattgatgc cagcaaatat ccaaaaccaa gtgatgagga attgaaaaag
    17641 accctgtcac ctgaggagta tgcagttacc caggaaaatc aaacagaacg agctttctca
    17701 aaccgttact gggataaatt tgaatccggt atctatgtgg atatagcaac tggggaacct
    17761 ctcttttcat caaaagacaa atttgagtct ggttgtggct ggcctagttt tacccaaccc
    17821 atcagtccag atgttgtcac ctacaaggaa gataagtcct acaatatgac gcgtatggaa
    17881 gtgcggagcc gagtaggaga ttctcacctt gggcatgtct ttacggatgg tccacaggac
    17941 aagggcggct tacgttactg tatcaatagc ctctctatcc gctttattcc caaagaccaa
    18001 atggaagaaa aaggctacgc ttatttacta gattatgttg attaatactc ttcgaaaatc
    18061 tcttcaaacc acgtcagcgt cgccttaccg tatgtagggt t
//
LOCUS       DAVBNO010000039        17991 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVBNO010000039 DAVBNO010000000
VERSION     DAVBNO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17991
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.039"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            331..1377
                     /gene="pheS"
                     /locus_tag="VQ846_001461"
     CDS             331..1377
                     /gene="pheS"
                     /locus_tag="VQ846_001461"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET3076025.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1377..1886
                     /locus_tag="VQ846_001462"
     CDS             1377..1886
                     /locus_tag="VQ846_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3076026.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1963..4365
                     /locus_tag="VQ846_001463"
     CDS             1963..4365
                     /locus_tag="VQ846_001463"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET3076027.1"
                     /translation="MLISYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIETDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDNF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     RVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAEG
                     AGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEAALQPATPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(4433..5434)
                     /locus_tag="VQ846_001464"
     CDS             complement(4433..5434)
                     /locus_tag="VQ846_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET3076028.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5590..6037
                     /locus_tag="VQ846_001465"
                     /pseudo
     CDS             5590..6037
                     /locus_tag="VQ846_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6163..6429
                     /locus_tag="VQ846_001466"
     CDS             6163..6429
                     /locus_tag="VQ846_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3076029.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARNKQGISQKKLEEL
                     SGVSQPVIARMETGKTSPQLDTVLKVLASLGKILAVVRLEQGKS"
     gene            6689..8938
                     /gene="metE"
                     /locus_tag="VQ846_001467"
     CDS             6689..8938
                     /gene="metE"
                     /locus_tag="VQ846_001467"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET3076030.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYFFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9002..9868
                     /gene="metF"
                     /locus_tag="VQ846_001468"
     CDS             9002..9868
                     /gene="metF"
                     /locus_tag="VQ846_001468"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET3076031.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIVDLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEEHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10427..12640
                     /gene="pnp"
                     /locus_tag="VQ846_001469"
     CDS             10427..12640
                     /gene="pnp"
                     /locus_tag="VQ846_001469"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET3076032.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELITFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDHKPKKEFTETPKDSE"
     gene            12656..13273
                     /gene="cysE"
                     /locus_tag="VQ846_001470"
     CDS             12656..13273
                     /gene="cysE"
                     /locus_tag="VQ846_001470"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET3076033.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13285..14169
                     /locus_tag="VQ846_001471"
     CDS             13285..14169
                     /locus_tag="VQ846_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3076034.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFEREIASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14251..15594
                     /locus_tag="VQ846_001472"
     CDS             14251..15594
                     /locus_tag="VQ846_001472"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET3076035.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15587..15973
                     /locus_tag="VQ846_001473"
     CDS             15587..15973
                     /locus_tag="VQ846_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET3076036.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            15977..16861
                     /locus_tag="VQ846_001474"
     CDS             15977..16861
                     /locus_tag="VQ846_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET3076037.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGSQDYVFVLQQDKYTYVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            16998..17432
                     /locus_tag="VQ846_001475"
                     /pseudo
     CDS             16998..17432
                     /locus_tag="VQ846_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17740..17910
                     /locus_tag="VQ846_001476"
                     /pseudo
     CDS             17740..17910
                     /locus_tag="VQ846_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         5428 a         3446 c         4088 g         5029 t
ORIGIN      
        1 tcatcttatt tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga
       61 taggtcttgc aagaatggta aaaatctgat aaactaagtg gtaattgaat agaaatctga
      121 aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat
      181 atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga
      241 tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct
      301 ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg
      361 cttcgcgaag aaacgctgac tagcttgaag cagattactg ctggaaatga aaaagagatg
      421 caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg
      481 atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt
      541 gatgtcttga cagctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg
      601 gctcaactgg ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt
      661 caccgtcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt
      721 tatcaagtcg tggatggttt tgaagtggag caagactact ataactttga acgtatgaac
      781 cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc
      841 ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct
      901 aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg
      961 acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg
     1021 gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa
     1081 attcgtttgc gtccatctta cttcccattc acagagccat ctgttgaggt ggatgtttct
     1141 tgcttcaagt gtggtggaga aggctgtaac gtatgtaaga aaacaggttg gatcgaaatt
     1201 atgggagccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta
     1261 tactctggtt ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc
     1321 aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga
     1381 ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa caagcctttc
     1441 gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaggctt
     1501 atagtctgaa aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta
     1561 tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga
     1621 gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaaggat
     1681 ttggactagg taagcaactg tttgaattcg cacttgaact tgctacaaaa aatagttttt
     1741 cttgggcttg gctaggtgtt tgggagcata atacaaaagc tcaagcgttt tataatcgat
     1801 atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagta gatacggatt
     1861 ggttactgag aaagaaatta aggtaagaag aggattcttc tattgaaatg ataggaaagg
     1921 aaaagaacca gtgtggcaat ggtcattctg gagaactaaa ttatgcttat atcttataaa
     1981 tggttaaaag aattggtgga cattgatgtg ccatcacaag agttggctga aaaaatgtca
     2041 actacaggaa tcgaggtcga aggtgtcgaa tcaccagctg ctggtctctc aaaaattgtc
     2101 gtcggtgagg tcttgtcttg cgaagatgtg ccagagactc acctccatgt ttgtcaggtt
     2161 aacgttggcg aagaagagcg tcagatcgtt tgtggtgccc caaatgtgcg tgctgggatc
     2221 aaggtcatgg tggctcttcc aggagctcgt atcgctgata actacaaaat caaaaaagga
     2281 aaaatccgtg gtttggagtc acttggaatg atctgttcac ttggtgaatt gggaatttct
     2341 gactcagttg tgcctaagga attcgcagat ggcatccaaa tcttgcctga agatgccgtg
     2401 ccaggtgagg aagtcttttc ttacctagac ttggatgatg aaatcatcga actttccatc
     2461 acaccaaacc gtgcagatgc cctttctatg tgtggagtgg ctcacgaagt ggcagccatc
     2521 tatgacaagg cagtcaactt taaagaattt actctaacag aaactaatga agctgcggca
     2581 gatgcccttt ctgtcagcat tgagacagac aaggcgcctt actatgcagc tcgtatcttg
     2641 gacaatgtga ccatcgcacc aagtccacaa tggttgcaaa accttctcat gaacgaagga
     2701 atccgtccca tcaataacgt agtggacgtg accaactaca tcctgctcta ttttggtcaa
     2761 ccaatgcatg cctttgactt ggataacttt gaagggactg acatccgtgt gcgtgaagcg
     2821 cgtgctggtg aaaaattggt gaccttggac ggtgaagaac gtgacttgga cgtgaatgac
     2881 ctagtcatca ctgtcgcaga caagccagta gcccttgcag gtgtcatggg tggtcaagca
     2941 acagaaatct ctgaaaaatc tagtcgtgtt gtccttgaag ctgctgtttt caatggcaaa
     3001 tctatccgta agacaagtgg tcgcctgaac cttcgttctg agtcatcttc tcgctttgaa
     3061 aaaggaatta atgtggcaac agttaatgaa gcccttgatg cggcagctag cctgattgcg
     3121 gaacttgcag gtgcgacggt gcgtaagggc atcgtttcag cgggtgagct tgatacttca
     3181 gatgtagaag tttcttcaac ccttgctgat gttaaccgtg tcctcggaac tgagctgtct
     3241 tatgctgatg tagaagacgt cttccgtcgt cttggctttg gtctttctgg aaatgcagac
     3301 agctttacag tcagagtccc acgtcgtcgt tgggatatca caatcgaagc tgacctcttt
     3361 gaagaaattg ctcgtatcta tggttatgac cgcttgccaa ctagtctacc aaaagacgat
     3421 ggtacagcag gtgaattgac agccacacaa aaactccgcc gtcaagttcg tactattgct
     3481 gaaggagcag gtttgacaga aatcatcacc tatactctaa caactcctga aaaagcagtt
     3541 gagtttacgg ctcaaccaag taaccttacg gaactcatgt ggccaatgac tgtggatcgt
     3601 tcagtcctcc gtcaaaatat gatttcaggt atccttgata ccgttgccta caacgtggct
     3661 cgtaagaata aaaacttggc cctttacgag attggaaaag tctttgaaca aacaggtaat
     3721 ccaaaagaag aacttccaaa tgaaatcaac agttttgcct ttgccttgac aggcttggtt
     3781 gctgaaaaag atttccaaac agcagcagtt ccagttgatt tcttctatgc taagggaatc
     3841 cttgaagccc tatttactcg tttgggactc caagtaacct atacagcaac atctgaaatc
     3901 gctagccttc atccaggtcg tacagccgtg atttcactcg gtgaccaagt tcttggtttc
     3961 cttggccaag tgcatccagt cactgccaag gcttacgata ttccagaaac gtatgtggct
     4021 gagcttaacc tttcagctat cgaagctgcg cttcagccag cgactccatt tgtagaaatc
     4081 accaaattcc cggcagtcag ccgtgacgtt gcccttctcc tcaaggcaga agtgactcat
     4141 caagaagttg tagatgctat ccaagctgcc ggcgtgaaac gtttgacaga tatcaaactc
     4201 tttgacgtct tctcaggtga gaaattggga cttggtatga agtcaatggc ttatagcttg
     4261 accttccaaa atccagaaga tagcttaacg gacgaagaag tcgcacgcta tatggaaaaa
     4321 atccaagcat cgctcgaaga aaaagtcaat gcagaagtgc gttaaacgat agtatgatac
     4381 aaattccccc tctagtttat gaacactaga ggggtgattt tatataaaat aattaaaatg
     4441 tgatttctgt aaaaatacat ctatgatcag agttttgaaa acttacaatg tctaaatctt
     4501 taacatagta gtggttttta ggcaataaaa tatgatctat tgttgcatta aaaagttttg
     4561 gactttggct attccaagtt cctctttcaa aaggtggcag tacatttaat gcgtccctat
     4621 gagagcttat ttttgcaagt gctccatgac gcatagttgc attaaaatca cctgcaataa
     4681 tagcctttgg atattttgaa gccaattgat tatgaatgat gtttaagtct tgcttccaga
     4741 tttccattaa acctggcaga ggaggagcag tatgcaaggc aatgatatct ggtatatttt
     4801 gttttctcga atgtaataca attgtcccga accgtgttgt atgaaaagtt ttagcttctg
     4861 tatagaaacc ataacttttc ttgacaatca cagtcacagg agctattcca ctattggtag
     4921 gtggagaagt gaaaatatca tagttggcca tagaaagtcc aacttgatga aacaatagtt
     4981 tgattctctg gttttcttgc tcacctctga tattggtagc tagttcaggg aatatagcca
     5041 tatcggcgtc aaaatggcta aaaattcgct caatatgttg tgcttctatt tgattagcga
     5101 cgttccaagt tactaatttt aaggttttag tcttatttga tgcagaagaa agggttttat
     5161 ctgttccgaa taatagtaat atgagcgata gtaagagcaa aatgccagag taaaaccgta
     5221 tttgtttatt tttcttgtaa aaagatagga aaaaaaataa gaaacttaaa atagcagcca
     5281 tagttgcagt agctaagggg aaggcataaa tctgtgccaa gataggttgg gtagatagcc
     5341 aaatgttaag attagaagat gggaaaaaag aataagttat gatagggata acaaagagaa
     5401 tgaccgaaag ggcagagaga aatgttttaa tcattgtgtg agtacctcaa tttgcaatgg
     5461 ttgctcggag ctaatgtgac tttgtgactt tacttgattg atattctttt tcttgtcttc
     5521 ctcatagatt aagtacaacc caagtaaaaa aagtccaatt taagattgaa atgattaaaa
     5581 ggcacagaga tgcaagacaa ttatactaca aaaggtaaac atttgacaat cgatagccgt
     5641 cgcttaatcg aaagatggaa aaaagaagga aaatcaaata gagaagttgc ctctctactt
     5701 ggaaaagctc ctcaaactat ccacactgaa atcaagcgtg ggacagtccg aaaatgtctt
     5761 ggaaaagggc gcttcaaaga ggtttattct gccgactacg ctcaacagtc ttatgaaaac
     5821 aatcgcaagc gctcggtcaa gaaatcaagc ttgaccaagg aactaaagga aaagattctc
     5881 cactatacat aaccaaaaat tttcgcctga aatgatggtt atggctaaag gggttaacgt
     5941 gggaatttca accatttact attggattca tcgtggaaaa ttggggttaa gcaagcagga
     6001 tctgctttat cctagaaaag gaaaatcgct taagaaatag gctagcacca actttaaacc
     6061 cgctggtcaa tctatcgaat cataattcgc tcattttttt aaaaatacat agacgtaaaa
     6121 gaaagagggt taagtgatga agaatagtgc tattgggagt aattggaagg atgtccatct
     6181 gagctcttta gcaaggagga aatccttgaa agtgatatgc gagtagctat catgagcgag
     6241 ttgattgaag ccagaaataa gcaaggaatc agtcagaaaa agctagagga actcagtgga
     6301 gtgagtcagc ctgttatagc taggatggag acaggtaaga ctagtccaca gttggacaca
     6361 gtcttaaaag tcctagctag tctaggaaag atactagcag tcgtccgact tgaacagggg
     6421 aaaagttgac tagaatcagt tatagtcttt tctaataaca agccatagtc acttataaga
     6481 attactaata acgcgtttga gcattctaac taaaatacag ctaaaaacaa gttataaaag
     6541 ggatttgagg catgtgtctc aagtcttttt cttttgtcta aaatagaggt atagtttcaa
     6601 actatatcaa agcctaattt tttacaatta tacagatgct ttcttttctg ttaagatagt
     6661 ttcaacaaca aattttggag gacacatcat gtcaactaca atcatcggtt tccctcgttt
     6721 gggcgaattc cgtgaattaa aatttacaac tgaaaaatac tttagaaaag aaatctcaga
     6781 agaagaactt ttagcagcag caaaagactt gcgtgctaaa cactggaaca ttgtcaaaga
     6841 aaaaggcatc actgaaattc catcaaatga cttttctcac tatgataact tcctagatgc
     6901 ggctttcctt ttcaacgtgg tgcctgcatc cgttcaaaac ttggacttgt ctgaccttga
     6961 gcgctacttc gctttgggac gtggttacca gggagaaaaa ggggacgttc gcgcccttcc
     7021 aatgaagaaa tggttcaaca ctaactacca ctacatcgtt cctaaatttg aaaaagacac
     7081 tcaagtaaaa ttggctggtc acaagatttt cgatgagttc caagaagcaa aagaacttgg
     7141 attgaacact cgtcctgttc ttgtaggtcc attcactttc cttcaattgt cagactttga
     7201 agaaggcgtg aaagcagacg acttcgtaga tagcttagtg gctgcttacc aagaagtttt
     7261 tgctaaattg gctgaacttg gtgcgactcg catccaattg gatgaagcgg ctcttgtaaa
     7321 agacttgaca gctgaagaaa aagctctctt cttgaacctt tacaacaaac tcttggctga
     7381 caaaaaaggt cttgaagtct tgcttcaaac ttatttcggt gatgttcgtg acgtatacgc
     7441 tgaccttgta aacttgccag tagatgctat cggtcttgac tttgttgaag gtaagaaaac
     7501 tcttgaactc gttaaaggtg gcttcccagc tgacaagact ctctatgtag gtattgtcaa
     7561 tggtaaaaac atctggcgca acaactacga aaagagcttg gctgttcttg agcaaatccc
     7621 agctgaaaac atcgttttga caagctcttg ctcacttctt catgtgccat ttacaactgc
     7681 taatgaagaa tttgaaccag cactcttgaa ccactttgcc tttgcagttg aaaaattgga
     7741 tgaaatccgt gacttggatg ctatccgcaa tggtcaaggt tcagaagctc ttgcagccaa
     7801 caaagaactc tttgcgactg agcgtgttgg tgaaaatgct gaacttcgtg cgcgtatcgc
     7861 tggcttgaca gacgcagact acactcgttt gccagccttt gcagaacgtg aagctatcca
     7921 agaagaagct ttcaaacttc cagctcttcc aacaacaacg attggttcat tccctcaaac
     7981 aaaagaagtt cgtgccaaac gtttggctta ccgtaaaggt gaattgtctc aaaaagagta
     8041 cgatgctttc cttgctgaaa cgattgacga atggatcaag tggcaagaag atattgactt
     8101 tgatgtcctt gttcacggtg aatttgagcg taatgacatg gttgagtact tcggtcaaaa
     8161 cttgtcagga tacttcttct ctaaaaatgg atgggtacaa tcatacggta tgcgtggggt
     8221 aaaaccacca atcatctggg gtgatgtcac tcgtcttaac cctatcactg ttaaatggtc
     8281 tagctatgca caaagtcgta caaacaaacc tgttaaaggt atgttgactg gacctgttac
     8341 catccttaac tggtcattcc cacgtgaaga catctctatc aaggattcta ctcttcaaat
     8401 cgcccttgct atcaaggatg aagtgcttga ccttgaagct gctggtgtga aaatcatcca
     8461 aattgatgag gctgctcttc gtgaaaaatt gccactccgt cgtagtgact ggtacgaaga
     8521 ttaccttgac tgggcaattc ctgccttccg cttggtacac tcaacagtag cgtcagacac
     8581 acaaatccac actcacatgt gttactcaga atttacagat atcatcccag ctatcgataa
     8641 catggatgca gacgttattt cctttgaagc cagccgttca aaccttgaaa tcttggacga
     8701 actcaaagcg aaaaacttcc aaacagaagt gggacctggg gtttacgata tccactcacc
     8761 tcgtgtacca aacgaaggcg aaatcgacaa cacaatcgaa gccatccttg ctaaagtgcc
     8821 aagcaagaaa gtttggatca accctgactg tggtttgaaa acacgtggta tcccagaaac
     8881 aaaagaaagc ttgatccgcc ttgttgaagc agctaaagct gcgcgtgaga aattgtaaga
     8941 ttggatttct ctccatacag tggttgttca aaaagaaatc aactagaaaa ggttattaca
     9001 tatgtcacgc caaacaccgt cactctcatt tgaagtgttc cctccaaacc cagccgtggg
     9061 taatggtaac attatttcag ccttgcagga tatgcaggag ttggctcccc attttatcag
     9121 tgtaactgcc agcaataata aatttaatat caaggaaacg acggttcgtt tggctgactt
     9181 tatccaaaat gacttggcga ttccgaccat tgcccacttg ccagctattt atctaactaa
     9241 ggacaaggtt gctgaaacta ttgttgactt ggacaaagtt ggggttcaga aaatcttggc
     9301 tcttcgtggg gatattattc cagacgtaga accacaaaag gatttccgct atgcaaccga
     9361 cttgattgag tttatcaagg aacaagcccc tcactttgat attattggag cttgctatcc
     9421 agaagagcat ccagattcgc caaatcagat ttcagatatt caaaatctta agaagaaagt
     9481 ggatgcaggc tgttcgagcc tcgtaactca gctcttcttt gacaatgagc gcttctatga
     9541 ttttcaggac aagtgtatct tggctgggat tgatgttccc attcatgcag gaattatgcc
     9601 aattctgaat cgaaatcagg ctctccgact cttgaagact tgtgagaata tccatcttcc
     9661 acgcaaattt aaagccatct tagacaagta tgagcatgac cctgagtcgc tcagagcagc
     9721 aggacttgcc tatgcagtgg accaaatcgt ggacttggta actcaggatg ttgccggtgt
     9781 gcatctctat acgatgaata atgctgatac agcaaaatac atccatcaag caacccatgc
     9841 cttgtttaat caccagtctc taggataata aaagcaaacc attcttctca ggtgagggga
     9901 atggttcctt tttaatggta aagacctcac tttttaagaa aaatatgata aaataaattc
     9961 tgtacgtact tgatacaaag atgaaggtat taaataatac aatgcattta aactgtaaga
    10021 tttaacgaca cgagtgagtc aaatcgatgt tatttgacga gagagttagt aaagcattta
    10081 gctaatcgcc cgatagcgat tagcgtgaaa gctttagata ctttagtatt aaacctttct
    10141 aatgatgtat cttgaaaact gaacttgcct acaactctgt ggaaaaagat aaatctccct
    10201 aggagtagtc gctccgtcgt tcgatttcct atcttccttt gagttgcttg acggcttggt
    10261 atcttgagta attgaacacg gccgaaaagc tgtgtaaaaa agataaactg tcttgtcttc
    10321 atcgaagact tcgtcagttt cctattttta ctttgctttt gacgtccttg gtatcttgat
    10381 ctttgtaggc aaggcgtata atttcatcaa tcaaaagggg attaaaatgg caaaacaagt
    10441 gtttcaaacg acttttgcgg gtcgtgagtt aattgtagag actggtcagg ttgctaagca
    10501 agcaaatgga tctgttgtcg tacgttacgg tgagtcaact gtcttgactg ctgccgttat
    10561 gtctaagaaa atggcaactg gggatttctt cccactccaa gtcaactacg aagaaaaaat
    10621 gtatgcggct gggaagtttc ctggtggctt tatgaaacgt gaaggacgtc cttcaacaga
    10681 tgcgaccttg acagcgcgtt tgattgaccg tccgattcgt cctatgtttg cggaaggttt
    10741 ccgtaatgaa gtccaagtca tcaatacagt gctttcttat gatgaaaatg catctgcacc
    10801 aatggctgct atgtttggtt catctttggc actgtctatt tcagatattc catttgacgg
    10861 accaattgct ggggtacaag tgggatatgt agatggccaa atcatcatca acccaagtca
    10921 agaacaagca gagcaatctc ttcttgaatt gacagtagct ggaaccaagc acgctatcaa
    10981 catggtagag tctggtgcca aagaattgtc agaagaaatc atgttggaag cgcttcttaa
    11041 agggcacgaa gctgtcaaag aattgattac cttccaagaa gaaatcgttg ctgctgtcgg
    11101 taaagaaaaa gcagaagtgg aattgcttca cgtggatgct gaattgcaag ctgaaatcat
    11161 tgcagcctac aacagtgacc tccaaaaggc agttcaagta gaagagaaat tggcccgtga
    11221 agctgcaact caagcagtga aagaccaagt gactgccgtt tacgaagaaa aatatgcgaa
    11281 ccacgaagaa tttgaccgta ttatgcgtga tgtggctgaa atcttggaac aaatggaaca
    11341 cgcagaagtg cgacgtttaa ttacagaaga caaggtgcgt cctgatggtc gtaaggtcga
    11401 tgaaatccgt cctttggatg cggttgttga cttccttcct cgtgtacatg gttcaggtct
    11461 ctttactcgt gggcaaactc aagctctttc agtcttgacc ttggctccga tgggagaaac
    11521 tcaaatcatt gatggtttgg atccagagta caagaaacgc tttatgcacc actataactt
    11581 ccctcaatat tctgtagggg aaacaggtcg ttacggtgcg ccaggtcgtc gtgaaatcgg
    11641 tcacggtgct cttggtgagc gtgctcttgc tcaagtcttg ccaagcttgg aagaattccc
    11701 atacgctatc cgtctagtag cagaagtttt ggaatcaaac ggttcttcat ctcaagcttc
    11761 tatctgtgcg ggaactcttg cccttatggc tggtggtgtg ccaatcaagg cgccagtagc
    11821 tggtattgct atgggactta tctcagatgg aaataactac acagtattga cagatatcca
    11881 aggtttggaa gatcactttg gagatatgga cttcaaggtt gcaggtactc gtgatgggat
    11941 tacagccctt caaatggata tcaagattca agggattact gcagaaatct tgacggaggc
    12001 tcttgctcaa gccaagaaag cgcgttttga aatccttgat gtcattgaag caaccattcc
    12061 agaagttcgt ccagaattgg ctccaactgc tccgaaaatt gatacgatca agattgatgt
    12121 ggacaagatt aagattgtca tcggtaaggg tggagaaacc atcgacaaga ttatcgctga
    12181 aacaggtgtt aagattgata tagacgaaga aggaaatgtg tctatctact ctagtgacca
    12241 agatgctatt aaccgtgcca aagaaattat tgctggtttg gttcgtgaag ccaaagtgga
    12301 tgaagtttac cgtgctaaag tcgttcgtat cgagaaattt ggtgcctttg ttaacctctt
    12361 tgataagaca gatgcccttg ttcatatctc tgagatggct tggactcgta ccaatcgtgt
    12421 agaggatttg gtagaaatcg gggatgaagt tgatgttaag gttatcaaga ttgatgaaaa
    12481 aggccgtatc gatgcctcta tgaaggctct tctacctcgt ccgccaaaac ctgagcatga
    12541 tgaaaaaggt gaaaagtctg agcgccctca ccgcccacgt catcaaaagg atcacaaacc
    12601 taagaaagaa tttacagaaa caccaaaaga ttcagaataa gaaaaggaga aatgtatggg
    12661 gtggtggcgc gaaaccattg atattgtaaa agaaaatgat ccagcggccc gcaccacttt
    12721 ggaggttttg ctgacttatc caggtgtcaa ggccttggcg gcccaccgtc tctcgcattt
    12781 tctctggaag cacggcttca aactattagc tcgtatgtac agtcagtttt ggcgcttttg
    12841 gactcagatt gagattcatc caggagccca gattgattca ggtgttttta ttgaccatgg
    12901 ttctggtctg gtgattggag agacagcgat tgttgaaaaa ggcgttcttc tctatcacgg
    12961 agtgactctc gggggaacag ggaaagactg tggcaaacgc catccgactg tacgaaaggg
    13021 agccctcata tcagcccatg cccaagttat cgggcctgtg gaaattggtg aaaatgccaa
    13081 ggtcggtgct gcagcagttg tcgtagcaga tgtacctagt gacgtgacgg ttgtcggtat
    13141 tccggccaag attgtccgtc ttcatggtaa gaaagatgag cctgtcattc acgaagtcga
    13201 agaaaaacga gagtattatg tcaataaact cgagcaggct aaagatgcca gtcacagatc
    13261 gtctggtttg tagaggatac ctatatgatt caagcaagaa acaagttaag ccaagaggag
    13321 ctatctgaag cgaaaaaagt aattaactgt tgccaaaact atgacggtac ctatcgcgat
    13381 ccctatctct ctaacatgct taattttgac ccaaacatgc ccgccttttt cctttattat
    13441 gaaaaaggcg aacttgttgg tttattaact gtctatgcag atgaccaaga tgtagaagta
    13501 acgatactgg ttcatccagg tcatcgccgt caggggattg cgcgtgcatt gtttactagt
    13561 tttgagagag aaatagcttc tttccccatt cggtcagtca cttttcagac agaacgtatt
    13621 tttttagaga atcatcctga ctttgccagt aactggggac taatcgagga tgaagagaca
    13681 gaaacttggt taggtaagga tagaagacct tatccgttag caaatgtttc ccatcttgaa
    13741 gttttgttag cagatagttc gtatcaggat caaattagtc aattaaaatt tcaggcattt
    13801 tcagaggaac atgaatcgag agaagttgtg gatagatatg tcgctgaagc tctgaaggat
    13861 ccagaaagtc gcttatatat tttattaaaa gacggtcagg ttattgggac ttgtacggtt
    13921 gatttatcga ctaatacgaa ttacttctac ggtttagcaa tattggaacc tgaacgtgga
    13981 aaaggctatg gaagctactt agcaaaatcc ctcgtcaacc aactaattga gcaaaatgac
    14041 aaggaatttc agattgcagt ggaagatagc aatgtaggtg ccaaacgttt gtatgaaaaa
    14101 attggctttg tcaaacagac tcaggtggtt tatctgaatg agaaaggagc aagggattcc
    14161 gaagtgtaga gatattcgga ctgaaattta cttgaacttt tagtgatgaa actaattgtt
    14221 cttggatttc agctttcctg attatgattt atgattaaaa tctatgacac catgtctcgt
    14281 gatttgcgag aatttgtccc gattgaggac ggcaagatca agatgtatgt ttgtgggcca
    14341 acggtgtaca actatatcca cgtgggaaac gcccgttcga cggtagcttt tgatacgatt
    14401 cgtcgctatt ttgagtaccg tgggtataag gttgcctata tttccaattt tacagatgtg
    14461 gatgataaga ttatcaaccg tgccagggaa gaaggcatca cgcctcagga ggttgcggat
    14521 aagtacatcg ctgcctttcg tgaggatgtg acggccttgg gcgtgaaacc tgcgactcgc
    14581 catccgcgtg tagtggagtt tatggcagac atcatccgtt ttgtggaaga cttgatcgag
    14641 aaaggctttg cctatgagag tcaaggggat gtctatttcc gtgtagaaaa atcccacaac
    14701 tatgctaaat tggctaataa aaccttggaa gatttggagc taggtgcttc aggtcgtacc
    14761 gatgaagaaa cggctcgtaa ggaaaatcct gtagactttg ctctatggaa atcttccaaa
    14821 ccaggtgaga tttcttggga tagtccttgg ggacctggac gtccgggttg gcatattgag
    14881 tgttcagtca tgtcgacaga gattttaggt gataccattg atatccacgg tggtggagct
    14941 gacctagagt ttccacacca caccaatgaa attgcccaat ctgaagctaa aacaggcaag
    15001 gcttttgcta actactggat gcacaatggc tttgtcaata tcgacaatgt caaaatgtct
    15061 aagtccttgg gtaacttcat tacggtacac gatgccctta aaactcttga tgggcaagta
    15121 cttcgtttct tctttgcgac ccagcactac cgtaagccta tcaacttcac ggaaaaggca
    15181 gttcgcgatg ccgagaccaa tctcaagtat ctgaagaata cttacgaaca accatttaca
    15241 ggaaacgtgg atgcccaaga gttacaaaac tttaaagata agtttgtagc agctatggat
    15301 gaagatttta atgctgccaa cggtatcaca gttgtctttg aaatggccaa atggatcaac
    15361 tcagggaact atgatgcaag tgtcaagcaa gctcttgcag atatgttaga aatttttgga
    15421 attgtctttg ttgaggaagt tttggatgca gagattgaag acttgattca aaaacgccaa
    15481 gaggcgcgtg ccaatcgaga ctttgagaca gcagaccaaa tccgtgacca attggttact
    15541 caaggaatta agctccttga taccaaggat ggagtgaggt ggacacgtga ttgatgtcaa
    15601 tctcattaac gggattgcgc tagcctttga gggggatgcg gtgtattcta tgtatattcg
    15661 ccgtcacctc atcctcaaag gtatgaccaa acccaataaa ctccatcaag aagcaaccaa
    15721 gtatgtgtca gccaaggctc aggctcgcct gattgctctc atgttggagg agcaggtcct
    15781 aacggaaaaa gaagaagaaa tctacaaacg tggccgcaat accaatagcc acacaaaggc
    15841 taaaaatgca gatgtcgtga cttatcgtat gtccacggga tttgaagcgg ttatgggcta
    15901 tctccatatg actgagaatc tggaacgtct tgagagtttg gtttcatggt gcatccaaaa
    15961 agtggagggc tagaacatga gggaaaaaga actacaagac tggtttcctg aggctcggat
    16021 ttcagaccaa ccagtagaga aagagggcta tctcacgctc cctttagctt ctcagcagtg
    16081 gattttgctg gaggaagctg ggctcagcga gcgtgaaaag cagttggttg cccttttgac
    16141 ccagcaggag caggctcgtt cgctaaaccc ttggtattcc tatctggttg aaggcaaggg
    16201 acaggcaccg caagttttta aaaagattca gttggtttat tgccatcttt cttattttca
    16261 gcaggaaaat ctggcttctt ggctagatat gatgcggact ctttttccga attgtcagac
    16321 agtgctacag gtcggatctc aggattatgt tttcgtgctt caacaagaca aatacactta
    16381 tgtaagagat attttaagtg atacgattga agcggttgag tatgactttg gacttcgtct
    16441 ttctatcatg ttgggtcagg tttggtctca gacgggacat caagccctat cagacttaat
    16501 caaagctgag cgggatttgt tcaagacatg gtggcgtcag ggtcaccaag gtgttcatac
    16561 tttttctcag ctctatcttt ggagtatggg agaaagactc gtggacttga agccaatcaa
    16621 ggaatgtcta caccagatga ttttggatca agatcagatt caggaaatca ttctctctct
    16681 ttgggaaaat agtgctgttc tcactaaaac agcccagcaa ctctatctgc accgcaattc
    16741 tctccaatac aagattgata aatgggaaga gttgacaggg cttcagttga aagagttgac
    16801 cgacctgacc ttgtgttatc aattgatttt aggttctttg tcaactatag ttggtttgta
    16861 aagaagttaa tatttggaga agaggatggc catcttctcc atttttatgt gcagagttat
    16921 agtggcttga tgctgggaaa gtacactgtg actgctaaaa cattcctaga agttgctttg
    16981 atttccctaa tctatttatg tgaattttat gttattttac tataacagtt gttgctaagc
    17041 taaataatag tggggaagtt tatttagacg taagcattga agataatcag ggaggtagtg
    17101 ggtataattt cagttctgtt tcaagtagct cacaaacagc taaatatgaa ggaactggtt
    17161 ataataacaa ttcatcatta tatataacga ttgataaaac gtctgatgca acagctcttt
    17221 tgaaattaaa gttgaataat gttgataatc aacctgctac tgaagttcct agttcaggaa
    17281 ttactgtaaa attaaatgct aaagataatg ctggaaactg gacaagtgct tcgaataaaa
    17341 aagaagtaac agtaaaaatt gtttctgcta aaccgacata tccagacaaa attttagtga
    17401 aaaatcctga taatataaaa gatacagaaa aaaatgccat tattgaaaaa ttgaaagagg
    17461 caaataaaaa tcatccagca ggagctccaa cctttgctaa aggtgaagga gagcatgcaa
    17521 atgatattgt agcaacttat tcagatggta caacttatta tgtaccgtta aatgatgtga
    17581 caaaatatgc gaggtagtgg ctgtaccact cacttattca cctccccgtg atttgtagta
    17641 gtgataggct ttctcactat tattataaaa caaaataaag atcacaacac tttttcattc
    17701 tgtgttgtgc cttgagtgaa acgaaaggaa tgaattataa atatgaaaag tatagtcact
    17761 aacatagatg agcgcttgcg tactcgccta cgagtgatta tctggaagca atggaagaag
    17821 aaatcgagac gattatgggg attgcttaag ttaggggttc ctaaatggat agcagataag
    17881 gtatctggct ggggcgagca ctatcaatta tattaaaatg aaataagaat aggacgaatc
    17941 gatcagggaa gtcaaatcga tttctaacaa tattttagaa gtagaggtat a
//
LOCUS       DAVBNO010000040        16753 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVBNO010000040 DAVBNO010000000
VERSION     DAVBNO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.040"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            80..544
                     /locus_tag="VQ846_001477"
     CDS             80..544
                     /locus_tag="VQ846_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET3076038.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            702..1127
                     /gene="lacA"
                     /locus_tag="VQ846_001478"
     CDS             702..1127
                     /gene="lacA"
                     /locus_tag="VQ846_001478"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET3076039.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            1158..1673
                     /gene="lacB"
                     /locus_tag="VQ846_001479"
     CDS             1158..1673
                     /gene="lacB"
                     /locus_tag="VQ846_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET3076040.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1684..2613
                     /locus_tag="VQ846_001480"
     CDS             1684..2613
                     /locus_tag="VQ846_001480"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET3076041.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYCASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFTKHGDTFYKVDIPRIQAVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2615..3595
                     /gene="lacD"
                     /locus_tag="VQ846_001481"
     CDS             2615..3595
                     /gene="lacD"
                     /locus_tag="VQ846_001481"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET3076042.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3829..4665
                     /locus_tag="VQ846_001482"
     CDS             3829..4665
                     /locus_tag="VQ846_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET3076043.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4701..5018
                     /locus_tag="VQ846_001483"
     CDS             4701..5018
                     /locus_tag="VQ846_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET3076044.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5018..6709
                     /locus_tag="VQ846_001484"
     CDS             5018..6709
                     /locus_tag="VQ846_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET3076045.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            6924..8330
                     /gene="lacG"
                     /locus_tag="VQ846_001485"
     CDS             6924..8330
                     /gene="lacG"
                     /locus_tag="VQ846_001485"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET3076046.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNVKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDPKNPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTTLEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIIRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8420..8632
                     /locus_tag="VQ846_001486"
     CDS             8420..8632
                     /locus_tag="VQ846_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET3076047.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWICHSDDELEEFKN
                     LFLNFINPEEWDTISFDSDFMPFQQS"
     gene            complement(8911..9672)
                     /locus_tag="VQ846_001487"
     CDS             complement(8911..9672)
                     /locus_tag="VQ846_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET3076048.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9854..10816)
                     /gene="nrdF"
                     /locus_tag="VQ846_001488"
     CDS             complement(9854..10816)
                     /gene="nrdF"
                     /locus_tag="VQ846_001488"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET3076049.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(11004..13163)
                     /gene="nrdE"
                     /locus_tag="VQ846_001489"
     CDS             complement(11004..13163)
                     /gene="nrdE"
                     /locus_tag="VQ846_001489"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET3076050.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13244..13462)
                     /gene="nrdH"
                     /locus_tag="VQ846_001490"
     CDS             complement(13244..13462)
                     /gene="nrdH"
                     /locus_tag="VQ846_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET3076051.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            13813..14076
                     /locus_tag="VQ846_001491"
     CDS             13813..14076
                     /locus_tag="VQ846_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET3076052.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            14082..15815
                     /gene="ptsP"
                     /locus_tag="VQ846_001492"
     CDS             14082..15815
                     /gene="ptsP"
                     /locus_tag="VQ846_001492"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET3076053.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            16070..>16753
                     /gene="phtA"
                     /locus_tag="VQ846_001493"
     CDS             16070..>16753
                     /gene="phtA"
                     /locus_tag="VQ846_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET3076054.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAA"
BASE COUNT         5215 a         3020 c         3477 g         5041 t
ORIGIN      
        1 tggtattatc aatcatgaac taggattcta gttttgcagg aagtctaata aagaaactag
       61 caaggtcagg ccttacttta ttggtggaca tccaggattt aattgtcttc tacttgatga
      121 tgaagtctat gaagattatt atctagagtt tgagaaagaa gagatttgct ctgttccacg
      181 tcctttccca gaaacaggta tgttggattt ccaagataga agtccatggc tagagggaca
      241 aaaagaaata gatcttagtt atgatctttt cagcacagat gcagtgactt tggatgaatt
      301 gcaatctcga acaattgccc ttcgttctct taaacatgat aagggattga aagtgcattt
      361 tgcagaattt ccaaacctca tcatctggtc aactttgaac aagggacctt tcattacctt
      421 tgaaccatgg tctggcttgt caacattcct tgaagaagga gatcatttag aagataagaa
      481 gaatgtttgt ctcttagaag ccaatcaggt tgaagaatta gggtttgaga tagaagtttt
      541 ataaaaaaag taaaaaataa ataacaaaaa caaacacaaa ctgttgactt cttcaaacaa
      601 tagtagtata ttatgtttgt gaggactaat tgactgtttt ttaaagacag ccaatacact
      661 gctggaatcc agcatagaaa aaataaaaag gagtatacaa tatgtctatt gttatcggtg
      721 cagatgctgc aggtttgaga ttgaaagaag ttgtgaaaga cttcttagaa aaagaaaact
      781 tccaccttgt ggatgttaca gctgaaggtc aagactttgt tgatgtgact ctcgctgttg
      841 ctgcagaagt aaacaaagaa gaacaaaacc ttggtatcgt gattgatgct tatggagctg
      901 gtccatttat ggttgcaact aagatcaaag gaatggttgc tgcagaagta tctgacgaac
      961 gttcagctta tatgactcgt ggccacaaca actcacgtat gatcactatg ggggcacaac
     1021 ttgttggtga tgaattggct aaaaacatcg ctaaaggatt tgttaatggt aaatacgacg
     1081 gtggtcgtca ccaaatccgg gttgacatgt tgaacaaaat gtgctaatta gattatagta
     1141 agaaaggtaa gttaaaaatg agaattgcaa ttggatgtga ccacatcgta acagatgaaa
     1201 aaatggcggt ttcagaattt ttgaaatcaa aaggatatga agtcattgac tttggtacct
     1261 atgaccatac acgtactcac tacccaatct ttggtaaaaa agtaggggaa gcagtaacta
     1321 gtggtcaagc tgatctcggg gtatgtatct gtggtactgg tgttggtatc aacaacgctg
     1381 taaataaagt tccaggtgtt cgttctgcct tggttcgtga tatgacaaca gccctttatg
     1441 ctaaagaaca attgaacgcc aacgttatcg gttttggtgg taagattact ggtgaattgc
     1501 ttatgtgtga tatcatcgaa gctttcattc atgctgaata caaaccatct gaagaaaaca
     1561 aaaaattgat tgcgaaaatt gaacaccttg aaagtcacaa tgctcaacaa acagacgcaa
     1621 acttcttcac tgaattcctt gaaaaatggg atcgtggaga ataccacgac taagaggtaa
     1681 cctatgattt taacagttac aatgaaccca tccattgata tttcctatcc cttggatgag
     1741 ttgaagattg atactgtcaa tcgtgtggtg gatgtaacca aaacggctgg tggtaaggga
     1801 ctcaatgtta cccgagtact ttcagaattt ggcgattctg ttcttgctac tggtttagtg
     1861 ggtggcaaac ttggtgagtt tttggttgaa catatcgata atcaagtaaa gaaagatttc
     1921 ttctcaattc agggagaaac tcgtaactgt atcgctattc tccacggaga caaccaaaca
     1981 gaagttcttg aaaaaggtcc tgaagtattg gaacaggaag gtcaagactt tttggaacat
     2041 ttcaaaaaac tcttggagtc agttgaagta gtggccatct caggtagtct gccagctggc
     2101 cttccagttg attactgtgc gagcttggta gaacttgcta atcaagctgg caagcctgta
     2161 gtcttggact gctcaggtgc agcacttcag gctgttcttg aatcacccca taaaccaaca
     2221 gtcatcaaac caaataatga agaattgtct cagcttcttg gaagagaagt ttctgaggat
     2281 ttggatgaat taaaagaagt acttcaagaa cctttgtttg cagggattga atggattatc
     2341 gtttcacttg gtgccaacgg tacttttacc aaacatggtg acactttcta caaggtagat
     2401 attcctagaa ttcaggcggt aaatcctgtt ggatctggag actctactgt ggcaggaatt
     2461 tcttcaggac ttcttcacaa agaatcggat gcagaattac tcatcaaggc aaatgtcctt
     2521 ggtatgctca atgctcaaga aaaaatgact ggtcatgtca acatggccaa ctatcaagct
     2581 ctatatgatc aattaatagt aaaagaggta taaaatggct ttaacagaac aaaaacgtgt
     2641 acgcttagaa aaactttctg atgaaaatgg tatcatctca gctcttgcat ttgaccaacg
     2701 tggtgctttg aaacgcctca tggctcaaca ccaaacagaa gaaccaactg tggcccaaat
     2761 ggaagaactt aaagttttgg tagcagatga attgactaaa tacgcttcat ctatgcttct
     2821 tgaccctgag tatggacttc cagcaactaa agctcttgat gaaaaagctg gtcttctcct
     2881 tgcttatgaa aaaacaggtt atgacacaac aagcacaaaa cgcttgccag actgcttgga
     2941 tgtttggtct gcaaaacgta ttaaagaaga aggtgcagat gcagttaaat tcttgcttta
     3001 ctatgatgta gatagctcag acgaactcaa tcaagaaaaa caagcctaca tcgaacgcat
     3061 cggttctgag tgtgtggctg aagatatccc attcttcctt gaaatccttg cttacgatga
     3121 aaaaattgca gacgcaggtt ctgtagaata cgctaaagta aaaccacaca aagttatcgg
     3181 tgctatgaaa gtcttttcag acccacgctt taacattgat gtcttgaaag ttgaagttcc
     3241 tgttaacatt aaatatgttg aaggcttcgc tgagggtgaa gtggtttaca cacgtgaaga
     3301 agcagcagcc ttcttcaaag cgcaagatga agcaacgaac ttgccataca tctacttgag
     3361 tgctggtgta tcagctaaac tcttccaaga tactcttgta tttgctcatg aatcaggtgc
     3421 aaactttaac ggagttcttt gtggccgtgc tacatgggca gggtcagttg aagcttacat
     3481 caaagatggt gaagcagcag ctcgcgaatg gcttcgcaca actggatttg aaaacattga
     3541 tgagctcaat aaagttcttc aaacaacagc gacttcatgg aaagaacgtg tgtaagaaag
     3601 tcctcctagt ttaggaacat gaatctaaaa aaatttaaaa aaagttgtat gtaaaggttt
     3661 acaaaataac ttacttgtgc tatacttaaa tcacaagtta atacaaggtg agtgttacta
     3721 agtaatatta ggcatgatca caggtgaatt agaaatcagc tgattttcta gttcatttgt
     3781 ggtcattttt tgtactcata tacctttaaa atataaaagg aggttgacat gtatcgaatt
     3841 ctaaatccaa tgaatcacaa tgtctcgctt gtcagaaatg ataagggaga agaggtgatt
     3901 gtaattggta aggggattgc attcggaaag aagaaggggg atttgattgc tgaaaatcag
     3961 gttgagaaaa tctttcggat gaagaccgaa gagtccagag aaaactttat ggctcttctc
     4021 aaagatgttc cgcttgattt tatcacagtg acctatgaaa ttattgataa gctatcaaag
     4081 aaatatcatt atccgattca agagtatctc tatgtaacct tgacagatca tatttactgt
     4141 tcttatcaag ctctaactca aggaaggtac aaggatagta atctgccaga tatttccgct
     4201 aagtatcctg tcgcttttca aatcgcaaat gaagcttttg aaatttaccg tcagaagcta
     4261 gcagatcatt ttcctgagga cgaaattatt cggattgctt atcatttcat taatgctgaa
     4321 ggtgaaaatg aagtggaact tgtggagtcg attgataaga ggaaagaaat tctcaggaat
     4381 gttgaagaag ttttaacgga ctatgcaatt caacgaacta aaaagaataa ccatttctat
     4441 gatcgcttta tgatccattt gaattatttc ttggattatt tagacagatc tagagatgat
     4501 aaccaatcac ttctggatat ggaagatcat attaaacaat cctatccaaa agccttcgag
     4561 attggttcca agatctatga tgtgattacg caacatacgg gtcttgattt gtataaaagt
     4621 gaacgagttt atctagttct acatatccaa cgtttattgt cataaaaatt tatttaaaac
     4681 tatataagga gaattctatc atgaatagag aagaagtaac attgttaggt tttgaaatcg
     4741 tagcctatgc tggcgatgct cgttcaaaac tattggaagc cttgaaggct gctgaagctg
     4801 gtgattttga aaaagcggac gctctggtag aggaagctgg tagctgtatt gcagaggctc
     4861 accacgcgca aacaagtcta ttgactaagg aagcttctgg tgaggactta gcgtatagtg
     4921 tgaccatgat gcatggtcaa gatcacttga tgacaactat cttgttaaaa gacctaatgc
     4981 accatttaat tgaactttac aagagaggag ttaaataatg aataaactaa ttgcatttat
     5041 cgagaaagga aagcctttct ttgaaaaact atctcgtaat atctatcttc gtgctattcg
     5101 tgatggtttc attgcaggta tgcctgttat tctcttctca agtatcttta tcttgattgc
     5161 ctttgtacca aactcatggg gctttaaatg gtctgatgaa gttgtagcct ttctgatgaa
     5221 accttatagc tattctatgg gtattctggc tctcttggta gctggtacaa cagctaagtc
     5281 attgactgac tcagtaaacc ggagcatgga aaaaaccaat caaatcaact atatgtcaac
     5341 attgttggca gcaattgttg gtttgttgat gttggcagct gatcctatcg aaagtggtct
     5401 agctactgga ttcttgggaa caaaaggttt gctttcagcc ttccttgctg ccttcgttac
     5461 tgtagccatc tataaggtct gtgttaagaa caacgtcact attcgtatgc ctgacgaagt
     5521 tccaccaaat atctcacaag tctttaaaga tgtgattcca ttcactctat cagtggtttc
     5581 tctttatgct cttgatttac tagctcgtca ttttgttggt tctagtgtgg cagaatcaat
     5641 cggtaaattc ttcgcaccac tcttctcagc agcagacgga taccttggta ttaccattat
     5701 ctttggtgcc tttgccttct tctggtttgt tgggattcat ggtccatcta tcgttgaacc
     5761 tgctatcgca gcgattacct atgcaaatgc tgaagtcaac ttgaaccttc tccaacaagg
     5821 gatgcatgca gacaagattc ttacttctgg tacacaaatg tttatcgtta ccatgggtgg
     5881 tacaggtgcg acattggtcg ttccatttat gttcatgtgg ttgacaaaat cgaaacgtaa
     5941 ccgtgcaatc ggacgtgctt cagtagttcc taccttcttc ggtgtaaatg aaccaatctt
     6001 gtttggtgca cctcttgttt tgaatccaat cttcttcatt ccatttatct ttgctccaat
     6061 tgcaaacgta tggattttca aattctttat tgaaactctt ggaatgaact cattcactgc
     6121 taatctacca tggacaactc cagctccact aggtctagtt cttggaacta acttccaagt
     6181 gctatcattc attcttgctg cccttctaat tgtggttgac gtagttattt attacccatt
     6241 ccttaaagtc tatgatgaac aaattcttga agaagaacgt tcaggtaagt ctaatgatga
     6301 attgaaagaa aaagttgctg caaacttcaa cactgcaaaa gcggatgcta ttcttgaaaa
     6361 agcgggtgtc gatgcagcac aaaataccat cactgaagaa acaaatgtcc tcgttctctg
     6421 tgctggtgga ggtacaagtg gactccttgc aaatgctttg aataaggcag cagcagaata
     6481 caatgtccct gtgaaagcag cagcaggcgg ctatggtgct caccgtgaaa tgttgccaga
     6541 gtttgatttg gttatccttg ctcctcaagt tgcttcaaac tttgaagata tgaaggcaga
     6601 aacagataag ctcggtatta aacttgctaa gacagaaggc gctcaataca tcaaattaac
     6661 tcgtgatgga aaaggtgccc tagcattcgt acaagagcaa ttcgattaag gctaggaact
     6721 gtgaaacagt ctcccatcgt tacggaaatc gctatggcga atttcctaat cgccttatta
     6781 attcgtcggt aaaaagatat cgtttttacc tcctcatgtc acaattcagt gacttggtac
     6841 aagaagtgag atggagaagc atggctcact gactcctctc ctctcacttt tactttattt
     6901 aaatcaagaa ataggtgaaa aaaatgacaa aaacacttcc aaaagacttt attttcggtg
     6961 gcgcaacagc tgcttatcaa gcagaaggtg ctacacatac tgatggaaaa ggaccagttg
     7021 cttgggataa atatcttgag gataactact ggtacactgc cgaaccagct agtgattttt
     7081 acaatcgata tccagttgac ctcaagctag cagaagagta tggtgtcaat ggtattcgaa
     7141 tttctattgc ctggtcacgt attttcccga ctggttacgg ccaagtaaat gttaaaggtg
     7201 ttgagtttta tcataattta tttgcagagt gtcacaaacg tcatgttgag ccctttgtaa
     7261 ctcttcatca ctttgacacg ccagaagctc tccattcaaa tggagacttc ttaaaccgtg
     7321 aaaatatcga acattttgta gactacgctg ccttctgttt tgaagaattt ccagaagtaa
     7381 actattggac aacctttaat gaaattggac caatcggtga tggtcaatat ttggttggga
     7441 aattccctcc aggtatccag tacgaccttg ccaaagtctt tcaatcacac cacaatatga
     7501 tggtgtctca tgcgcgtgca gtcaagctat ataaagataa aggctataaa ggggaaattg
     7561 gtgttgttca cgctctgcca accaagtatc ctttggatcc taaaaatcca gcagatgttc
     7621 gtgcagctga gttggaagat atcatccaca ataaattcat cttagacgca acttatctag
     7681 gtcgctattc agctgaaacc atggaaggtg tcaaccatat cttatcagtc aatggtggta
     7741 gtttggatct tcgtgaagaa gattttacaa cattagaagc tgcaaaagac ttgaatgatt
     7801 tcctaggaat caactactat atgagtgact ggatggaagc ctttgatgga gaaactgaaa
     7861 ttatccataa tggtaaaggt gaaaaaggaa gctctaagta tcaaatcaaa ggtgttggtc
     7921 gtcgtgtagc tcctgactat gtaccacgca cggattggga ttggattatc taccctcaag
     7981 gtttgtatga ccaaatcata cgtgtgaaga aagattatcc taactacaag aagatttaca
     8041 tcactgaaaa tggtctcggc tataaagatg agttcgttga taacactgtt tacgatgatg
     8101 gtcgtattga ttacgtgaaa caacacttgg agatcttgtc tgatgcgatt gcagatggag
     8161 ctaatgtaaa aggttacttc atttggtcat taatggatgt cttctcatgg tcaaacggtt
     8221 atgaaaaacg ttacggtctc ttctacgtag attttgaaac tcaagaacgt tatcctaaga
     8281 aatcagctca ctggtataag aaagtagcgg aaactcagat tatagactag tagaattagt
     8341 cattagatat agaattttag tgagtcaaaa agatgttcaa agattttatc caatctattt
     8401 atgaaaaaaa gtttatatta taaatttcga aaaatgctct caaataccgt gtttgacgag
     8461 tgaagaattg aaaagtcttg gaaaatggta tgtctcgact ggtaaagaat ggatttgtca
     8521 ttcagatgat gagctggaag aatttaaaaa tctattttta aattttatca atcctgaaga
     8581 atgggatact atctcctttg attcagattt tatgccgttt caacaatcgt aaccaatttc
     8641 tcaaaaaagt taaatcttat atttagtact ctgtaaaact cttatctaat cacgttgctt
     8701 atactcaatg aaaatcaaag agcaacttta aactaggaag cgagtcgcag atttctcaat
     8761 gcatagcttt gaggaattgg gcaaaaagtc tttgatatag aaaaacgcat agtatcaggt
     8821 gtttcaacac ctgatattat gcgttttatt gtgggaagat ttactttttt tcttctgaaa
     8881 ttgagttgtt acccaggctc tttcagttta ttaaggcttg atgactttaa tgtgtttaga
     8941 tagcttaaaa aggattgaat cacttagttt agaatctgaa acaatagtat caagatttga
     9001 tacattataa aaagtataaa aatcaaactt attgaacttg ctatgatctg cgagtaaata
     9061 ttttttatta gaattattta aagcgatgcg ttgagcctct ccctcttcct cgctaaaagt
     9121 agctagagct ccgttttgaa taccattaca gctaacgaaa gctttagaaa attggagatt
     9181 agagagattt tgtagggtca atgtaccaac aaaagcacct gtaatatcgc gataatttcc
     9241 acctattaaa atcaaatctg ttaattttcg ttcgcttaaa atcagaaaaa caggtagact
     9301 gttggttacg acgcggatat tgtcaatagg caactcacgc gcaaaaaact ctaatgttgt
     9361 tcctggtcca atgaaaatag tttctctttc ttctactaga ctgcctgcaa aatgggctat
     9421 ttcttgtttt tctgccgttt ggagggcttg tttttcaata tttgatcgct cattagtcaa
     9481 aagggagttg gttcgaagtt tttcagctcc accatgcaca cgaatcagca aatctttatc
     9541 agctaattcc tgtaaatagc gccttgcagt catatctgaa acggctattt cgtccataat
     9601 ctgtttaact gttatggttc ctttactatt tactatctct aaaattttgg ttaatttttc
     9661 ttgtttgagc atattatcac ctcgtttcct actactatct taccataaac aaactcatca
     9721 ttcaaataca aaaacaacaa aatgaaacaa aaacaaaaat atcgaagttt gttttcaaaa
     9781 ctttcgatat ttttgttggg ttataacttt gatgtttcta gtttactttt tgatgattga
     9841 gagtgatgga gaattagtct aaaccgtagt tatagtcatc gtcttgcatg gcttcaactt
     9901 cgccaagaag gtaaccattt ccgacttgag agaagaagtc atggttggaa gttcctgttg
     9961 aaataccgtt cataacgatt gggttgacat cttcagctga atctgggaaa agtggatctt
    10021 gtcccatgtt catgagagct ttattggcat tgtagcgaag gaaggtttta acctcttcag
    10081 tccaaccaac accgtcataa agactctctg tgtagccttc ttcattttca taaagagtat
    10141 agagtaggtc gtacatccat tctttgagtt tttcttgctc ttcttcaggc aattcattga
    10201 aaccaagttg gaatttgtaa ccaatgtagg ttccgtgaac agactcgtca cgaataatca
    10261 atttaatgat ttctgcaacg ttggctagtt tgttgttacc gagatagtag aggggagtga
    10321 agaaaccaga gtagaagagg aaggtttcga ggaagacgct ggcaactttc ttttcaagtg
    10381 ggctgccgtt taggtagatt tcgttgacaa tctcagcctt cttttgtagg taaggattgg
    10441 tattggtcca ttcgaaaatt tcttcaatct cagccttagt attcaaggta gaaaagattg
    10501 atgagtaaga tttagcgtgg acagattcca taaattggat gttattgaaa acagcttcct
    10561 catgtggtgt acggatgtct gcgcgaaggg cttgaacccc agtttcagat tgcatagtgt
    10621 caagaagggt taaaccacca aaaacttttc cgaccaagtc tttctctttg ttagatagct
    10681 ttctccagtc atccaagtcg tttgataagg gaatacgtgt atcgagccaa aattgctccg
    10741 tcagtttttc ccaagttgat ttgtcgatga catcttcgat ggcattccag ttaatggctt
    10801 tgtagtaagt ttccatttaa aatctctttc tgtgtttagt attgcgaact cacaattatt
    10861 tctactttac cataattcta taggagtatc gcacaaaaag tcggaagccc gacttttaaa
    10921 atgttacata aattatgtta tgacatagta gatttgattt tatcagtgct gcttagggaa
    10981 aaataatgtt tctatgctag aaactaaatc acacagcttt cacattggtt ggcgccgact
    11041 tctccaccgt catctgtaaa ggtacggacg tagtagatag acttgattcc cttgttaaag
    11101 gcatagttac gaaggatgga caaatcacgt gtcgtttgtt tattttccct cttccattcg
    11161 taaaggcctt ttggaatgtc actgcgcatg aagagggtga gtgaaagtcc ttgatccacg
    11221 tgttcagtcg cagcagcgta aacatcgatg actttacgca tatccatatc gtaggcagaa
    11281 gtgtagtaag gaatggtttc tgtagacaag ccagcagcag ggtagtagat tttaccaatt
    11341 ttcttctctt ggcgttcttc gatacgttgc gtaatcgggt ggatagaagc agaaacgtcg
    11401 ttgatatagc tgatagaacc atttggcgct acagcaaggc gattttggtg gtaaagacca
    11461 tcttcttgaa ccttgtcgcg aagttcagcc caatcagcaa caccagggat aaaaacattt
    11521 ttgaagagtt ctttaacacg gtctgatgtt ggaacaaatt caccagttac atacttgtca
    11581 aagtaacttc cgttagcata gtctgatttt tcaaagttgt ggaaggtaat accacgttca
    11641 cgcgcaatat tgtttgactc taccaaggtc cagtagttca taagcataaa gtagatgctt
    11701 gtaaattcaa cagactcagg tgaaccatat tcaatgagtt gttgggcaag gtagctgtgc
    11761 agtcccatgg caccgagacc aaaggtgtgg gcttggctat ttccatggtc aatcgttggt
    11821 acagctacga tatgtgaact atctgtaacg aaagtaaggg cacgaaccat agcacggata
    11881 gaacgaccaa aatcaggtga agtcatcatg ttaaccacgt tggttgaacc cagattacat
    11941 gaaacatctg ttcccatttg aaggaattct tgagcatcgt tgatcaagct tggttcttga
    12001 acttgaagaa tctcagaaca caagttactc atgataatct ttccatcaac aggatttgca
    12061 cggttagccg tatcgatgtt gactacataa ggatagccag actcttgttg caatttagag
    12121 atttcagttt ccaaatcccg cgccttgatt tttgtcttgc gaatatttgg atttgcgacc
    12181 aattcatcgt atttttcagt aatgtcgatg taattgaatg gcacaccgta ttctttttct
    12241 acagagtaag ggctgaagag gtacatttct tcatttttac gagccaattc gtagaattta
    12301 tcaggtacta caacaccaag tgatagagtc ttgacacgta ctttttcatc agcgttttct
    12361 ttcttagttg aaaggaaagc gatgatatct gggtgaaaga cgttgaggta gacaacacca
    12421 gcaccttgac gttgccccaa ttggttggag taagagaagc tgtcttcaaa aagcttcata
    12481 acaggaacga cacctgaagc agctccttca tagcctttga taggtgcacc agcttcacga
    12541 aggttgctga gggtaattcc cacaccacca ccaatacgtg aaagttgaag agctgagttg
    12601 atagaacgtc cgatagagtt catatcatcc gtcacttgga ttaggaaaca agataccaac
    12661 tccccacgac gagcacgtcc agcattcaag aaggaaggag tagcaggttg gtagcgttgg
    12721 tggatgattt cattggcaat atcgattgca acagcttcat tcccatcagc gaaataaagg
    12781 gcgttgaaga agacacggtc ttccatattt tcaagatagt attcaccgtc attagtcttt
    12841 aaggcatatt gattgtaaaa tttataagct gccatgaatg acttgaattg gaagttttgg
    12901 tctttgataa attgagccaa ttcttccaag aactctggac ggtatttctt gataaaggct
    12961 gtttcgatgt agttgtgttc aatgaggtaa ttgattttgt ctttgattga atcaaaaacc
    13021 atagtgtttg gaactacatt ttctttaaag aaagcatcca aggcttcctt atctttatga
    13081 agcatgattt gtccattaac aggacggtta atttcgttat taagacggaa gtaagtcacg
    13141 tcttcaagat gttttaatcc cataaaattt cccttatcta attacaaaag aaaggcttct
    13201 aagttagccc taaaagcagt ttcttctgga tgatgtacta agattatgct aattgtttca
    13261 gttttcctgg ttggaaacct gaaaagactt cagttggtgt ttggataaca ggagctgcgc
    13321 taaaaccgag ctctttaact tgatcgacgt actcaggttg ctcatcaaga ttgatttcac
    13381 gataagagac attattactg tccaagaaac gcttggtcat tttacattgg acacaattgt
    13441 ttttagaata aacggttacc attgtgtaac tcctcttcaa aatttaatac tatcttagta
    13501 tatcagaaaa taaaattttg tcaatgattt gaactaaata tagtggtgtt cttttgttgt
    13561 ttttatatat aaacacaata tatagtgttt gtattgtgaa atagcaatga ttatcctata
    13621 aattgttttt aaagaaacta tatcctgtgt tttgttatct aggatagaag attttcagca
    13681 agttatctga aaggaaattg aaggaaaatc cagaaaatgc ttgaaaaaaa tcctagaaga
    13741 tggtataata ctaaattgta agggttatca catataactc aaaaaaagaa agaacaaaag
    13801 gagagtcaaa ctatggcttc taaagatttc cacgtagtgg cagaaacagg tattcacgca
    13861 cgtccagcaa cattgttggt acaaactgct agcaaatttg cttcagatat cactcttgag
    13921 tacaaaggta aatcagttaa ccttaaatca attatgggtg ttatgagtct tggtgttggt
    13981 caaggtgctg acgtaactat ctcagctgaa ggtgcagatg cagatgacgc tatcgctgca
    14041 atctcagaaa caatggaaaa agaaggattg gcataaggga aatgacagaa atgcttaaag
    14101 gaatcgcagc atctgacggt gttgcagttg caaaagcata tctactcgtt cagccggatt
    14161 tgtcatttga gactattaca gtcgaagata caaacgcaga agaagctcgc cttgatgccg
    14221 ctctacaggc atcacaagac gagctttctg ttattcgcga gaaagcagta ggtacgctcg
    14281 gtgaagaagc agctcaagtt tttgatgctc acttaatggt tcttgctgac ccagaaatga
    14341 tcagccaaat caaggaaact atccgtgcga agaaagtgaa tgcagaagca ggtctgaaag
    14401 aagttacaga tatgtttatc actatctttg aaggcatgga agacaaccca tacatgcaag
    14461 aacgcgcagc ggatatccgc gacgtgacaa aacgtgtatt ggcaaacctt cttggtaaaa
    14521 aattgccaaa cccagcttct atcaatgaag aagtgattgt gattgcgcat gacttgactc
    14581 cttcagatac agctcaattg gacaaaaact ttgtaaaagc ttttgtaacc aacattggtg
    14641 gacgtacaag ccactcagct atcatggcac gtacacttga aattgctgct gtattaggta
    14701 caaacaacat cactgaaatc gttaaagacg gtgacatcct tgctgttaac gggatcactg
    14761 gagaagtgat tatcaaccca acagatgaac aagcggcaga atttaaagca gctggtgaag
    14821 cctatgcgaa acaaaaagct gaatgggcac ttttgaaaga tgctcaaaca gtgactgctg
    14881 acggtaaaca cttcgagttg gctgccaata ttggtactcc aaaagacgtt gaaggtgtta
    14941 acaacaacgg tgcagaagct gttggacttt accgtacaga gttcttgtac atggattctc
    15001 aagacttccc aactgaagat gagcagtatg aagcatacaa ggctgttctt gaaggaatga
    15061 acggtaaacc tgttgtcgtt cgtacaatgg atatcggtgg agataaggaa cttccttact
    15121 tcgatatgcc tcacgaaatg aacccattcc ttggattccg tgctcttcgt atctctatct
    15181 ctgagactgg agatgctatg ttccgcacac aaatccgtgc tcttcttcgt gcgtctgttc
    15241 acggtcaatt gcgtatcatg ttcccaatgg ttgcgctctt gaaagaattc cgtgcagcga
    15301 aagcagtctt tgatgaagaa aaagcaaacc ttcttgctga aggtgttgca gttgcggata
    15361 acattcaagt tggtatcatg atcgagattc ctgcagcggc tatgcttgca gaccaatttg
    15421 ctaaagaagt tgacttcttc tcaattggta caaatgactt gatccaatat acaatggcag
    15481 cagaccgtat gaacgaacaa gtttcatacc tttaccaacc atacaaccca tcaatcctac
    15541 gcttgattaa caatgtgatc aaagcagctc acgctgaagg taaatgggct ggtatgtgtg
    15601 gtgagatggc tggtgaccaa caagctgttc cacttcttgt cggaatgggc ttggatgagt
    15661 tctctatgtc agcaacatct gtacttcgta cacgcagctt gatgaagaaa ctcgacacag
    15721 ctaagatgga agagtacgca aaccgtgccc ttacagaatg ctcaacaatg gaagaagttc
    15781 ttgaacttca aaaagaatac gttaattttg attaatcgaa aagtccctgc aactcagtta
    15841 cagggacttt ttttgatatt ttaaaaagaa ttttcaagaa aatctttctt ataaaaagtc
    15901 caaccttgaa aaagtagtgg tcagaacaaa aaatacttaa atggttcata aaattcttga
    15961 caagttggag atttaggagt aaactattaa ccagttaagt aatagagagg agtttctgca
    16021 atttagaaat gaattgcaac tagaaatatc aaatagaaag agagtttcga tgaaaattaa
    16081 taagaaatac cttgttggtt ctgcggcagc tttgatttta agtgtttgtt cttacgagtt
    16141 gggactgtat caagctagaa cggttaagga aaataatcgt gtttcctata tagatggaaa
    16201 acaagcgacg caaaaaacgg agaatttgac tcctgatgag gttagcaagc gtgaaggaat
    16261 caatgctgag caaatcgtca tcaagataac agaccaaggc tatgtcactt cacatggcga
    16321 ccactatcat tattacaatg gtaaggttcc ttatgatgcg attttcagtg aagaattact
    16381 catgaaagat ccaaactata agctaaaaga tgaggatatt gttaatgagg tcaagggtgg
    16441 atatgttatc aaggtagatg gaaaatacta tgtttacctt aaggatgctg cccacgcgga
    16501 taacgtccgt acaaaagagg aaatcaatcg acaaaaacaa gaacatagtc aacatcgtga
    16561 aggtgggact ccaagaaacg atggtgctgt tgccttggca cgttcgcaag gacgctatac
    16621 tacagatgat ggttatatct ttaatgcttc tgatatcata gaggatactg gtgatgctta
    16681 tatcgttcct catggagatc attaccatta cattcctaag aatgagttat cagctagcga
    16741 gttggctgct gca
//
LOCUS       DAVBNO010000041        16590 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVBNO010000041 DAVBNO010000000
VERSION     DAVBNO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.041"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            49..537
                     /locus_tag="VQ846_001494"
     CDS             49..537
                     /locus_tag="VQ846_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET3076055.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            534..1721
                     /locus_tag="VQ846_001495"
     CDS             534..1721
                     /locus_tag="VQ846_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET3076056.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSTKEDNHFKVTVDQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            1714..2079
                     /locus_tag="VQ846_001496"
     CDS             1714..2079
                     /locus_tag="VQ846_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET3076057.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKMAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            2095..3441
                     /gene="asnS"
                     /locus_tag="VQ846_001497"
     CDS             2095..3441
                     /gene="asnS"
                     /locus_tag="VQ846_001497"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET3076058.1"
                     /translation="MMTKRVTIIDVKDYIGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMERTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            3594..3884
                     /locus_tag="VQ846_001498"
     CDS             3594..3884
                     /locus_tag="VQ846_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET3076059.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            3896..4366
                     /gene="ssbA"
                     /locus_tag="VQ846_001499"
     CDS             3896..4366
                     /gene="ssbA"
                     /locus_tag="VQ846_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET3076060.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            4398..4637
                     /locus_tag="VQ846_001500"
     CDS             4398..4637
                     /locus_tag="VQ846_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET3076061.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            4932..6087
                     /locus_tag="VQ846_001501"
                     /pseudo
     CDS             4932..6087
                     /locus_tag="VQ846_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            6089..6448
                     /locus_tag="VQ846_001502"
     CDS             6089..6448
                     /locus_tag="VQ846_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET3076062.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            6502..7251
                     /locus_tag="VQ846_001503"
     CDS             6502..7251
                     /locus_tag="VQ846_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET3076063.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            7241..7540
                     /locus_tag="VQ846_001504"
     CDS             7241..7540
                     /locus_tag="VQ846_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET3076064.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            7611..8546
                     /locus_tag="VQ846_001505"
     CDS             7611..8546
                     /locus_tag="VQ846_001505"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET3076065.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            8632..9144
                     /locus_tag="VQ846_001506"
     CDS             8632..9144
                     /locus_tag="VQ846_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET3076066.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            9181..9776
                     /locus_tag="VQ846_001507"
                     /pseudo
     CDS             9181..9776
                     /locus_tag="VQ846_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            9877..10071
                     /locus_tag="VQ846_001508"
     CDS             9877..10071
                     /locus_tag="VQ846_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET3076067.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(10117..11562)
                     /locus_tag="VQ846_001509"
     CDS             complement(10117..11562)
                     /locus_tag="VQ846_001509"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET3076068.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HSSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            11668..13290
                     /locus_tag="VQ846_001510"
     CDS             11668..13290
                     /locus_tag="VQ846_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET3076069.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALVLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            13493..15451
                     /locus_tag="VQ846_001511"
     CDS             13493..15451
                     /locus_tag="VQ846_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET3076070.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVVSKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGIESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            15623..16183
                     /locus_tag="VQ846_001512"
     CDS             15623..16183
                     /locus_tag="VQ846_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3076071.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILLLSNISEKYSRRLQEEIGFLTG"
     gene            16184..16558
                     /locus_tag="VQ846_001513"
     CDS             16184..16558
                     /locus_tag="VQ846_001513"
                     /inference="COORDINATES: protein motif:HMM:NF012869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076072.1"
                     /translation="MTEKIQEHELIKTNQAEKSVQDVLDNCIERVQNNSLKSDRVTSF
                     ETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLLNPISNIANFVTVY
                     SRSKGSSVALENLLAVPKEKFE"
BASE COUNT         4927 a         3054 c         3686 g         4923 t
ORIGIN      
        1 ttcttatatt cacttgtaat aaagcttaat agatggtaaa atagacgagt gaaaaaaaga
       61 caaaacaaag caaaaaataa tctactgtgg cagtatggtc tagggatgac gattttgttt
      121 gtggttatca gtgcttcctt tctgtatatg gtttctctta gcatgaaacc ctatcaaaca
      181 gctaaaagtg aaggagaaaa attagctcag cagtatgcag gattagagca ggccgatcag
      241 gttgatttat acaatggctt ggaatcttat tacagcgttc ttggtcgtaa taaacagcaa
      301 gaagcacttg ctgttctgat tggaaaagat gatcataaga tttacgttta tcagctaaat
      361 cagggtgttt cacaagaaaa agcagaaacg gtttctaagg aaaagggagc tggcgaaatt
      421 gacaagatta tctttggtcg ttatcaagat aagccaatct gggaagtcaa gtcaggatct
      481 gatttttatc tagtagattt tgaaacagga gcattggtca acaaggaggg cctatgaaat
      541 tatccaaccg tgttttagaa atggaagaaa gtgtgacttt ggctgctgga gcccgtgcca
      601 aagcactgaa ggctgagggc agagatattt tgtctctaac cttgggtgag ccagatttca
      661 ctactcccaa aaatatccaa gatgccgcca ttgcatcgat tcgagatggg cgtgcttctt
      721 tttatacagt aacctcaggt ctgccagagc ttaaggcggc ggtcaatagc tactttgagc
      781 gcttttacgg ctattctgta gcgtcaaatc aagtgacagt cgctgcggga gccaaatatt
      841 ctctctatac cttctttatg gctgtggtca atccaggtga tgaagtcatc atcccaaccc
      901 catactgggt tagctatgga gatcaggtca agatggcaga aggcgttccc gtctttgttt
      961 ctactaagga agacaatcac tttaaggtga ccgtagatca gttagaagca gctcgcactg
     1021 acaagaccaa ggttttggtg ctgaattcgc catctaatcc cacaggtatg atttacaccc
     1081 gtgaggaact cttggcaatt ggaaactggg ctgtagaaaa tgatattctc atcctagcag
     1141 atgatatcta tggccgcttg gtttataatg gtcatgagtt cacaccgatt tctagcctat
     1201 cggaagcgat tcgcaagcaa acagtggtca tcaatggtgt gtctaaaacc tatgccatga
     1261 ctggttggcg gattggttat gccgttggag aagcagacat tatcgctgcc atgtccaaga
     1321 ttgcaggtca aacaacttcg aatccgtcag cagtagccca atatgcagca gttgaggctc
     1381 tatcaggtga gcaagatact gtagaaagca tgcgtcaggc ctttgaggaa cgtcttaata
     1441 ctatctatcc cctccttgca gaggtgccag gatttgaagt ggtcaaaccg caaggggcct
     1501 tctatctctt cccaaatgtc aaaaaggcca tggagatgaa aggctacacg gatgtgacag
     1561 actttacaac tgctatctta gaagaagccg aagtggcctt ggtaacagga gctggatttg
     1621 gagcaccaga aaatgtccgc ctcagctatg cgacagacct agacacgctt aaagaagcag
     1681 tcgaacgctt gaaagcattt atgggtagtg agaatgattg atcattttga gattaaggta
     1741 aaggatttac aaatttcaga aggattttat aggagttttc tcgctccttt ggactataaa
     1801 atggctttta agactagttc tctaattagt tttctatccc cgaacagccc tcatcctggt
     1861 ggtgattttt ggctgactca gggaacacaa gatcctgttc attttgcttt tttagcagaa
     1921 aataaggaag aagttcaggc ttgttatgag gctggcttag aggcaggtgg gcgagacaat
     1981 ggggttcctg gttatcgaag tgagcatccg atttattatg ctgcttttat gattgacctg
     2041 gatgggaaca atatagaggt ggtttgccat aaagaataaa aaagaaaagg aaaaataatg
     2101 acaaaacgtg taacgattat tgatgtaaaa gactatattg gtcaagaagt gacgattggc
     2161 gcttgggttg ccaacaaatc aggaaaaggg aaaatcgctt tcttacaatt gcgtgatgga
     2221 acagccttct ttcaaggtgt ggcttttaaa ccaaactttg tcgaaaaatt tggtgaagaa
     2281 gtgggacttg agaagtttga tgttatcaaa cgcttgagcc aagaaacgtc tgtttatgtg
     2341 acaggtattg tcaaagagga cgaacgttct aaatttggct atgagttgga catcacagac
     2401 atcgaagtga tcggtgaatc tcaagactac ccaatcacac caaaagaaca cggaacagac
     2461 tttttgatgg ataaccgtca cttgtggcta cgctctcgta agcaagtagc tgtgttgcaa
     2521 atccgtaacg ctattatcta tgcaacttat gagttctttg acaagaacgg ttttatgaag
     2581 tttgacagcc caattctttc aggaaatgcg gcagaagatt ctacagaact ctttgaaact
     2641 gactacttcg gaacgccagc ctacttgagc caatcaggtc aactttacct agaagcaggg
     2701 gctatggctc ttggtcgtgt ctttgacttt ggtccagttt tccgtgctga aaaatcaaaa
     2761 acacgccgtc acttgactga gttctggatg atggatgctg agtactcata cttgacacat
     2821 gatgagtcgc ttgacttgca agaagcttat gtgaaagctc ttctacaagg tgttcttgac
     2881 cgcgcgcctc aagccttgga aaccttggaa cgtgatacag aactcttgaa acgctacatt
     2941 gcagagccat tcaaacgtat cacttacgat caagccattg acctcttgca agagcatgaa
     3001 aatgatgaag atgctgacta cgagcatctt gagcatggtg atgactttgg gtcaccacac
     3061 gaaacttgga tttcaaacca ctttggtgtg ccaacatttg tcatgaacta tccagcagcc
     3121 atcaaggctt tctacatgaa accagttcct ggaaatccag agcgcgtgct ttgtgcagac
     3181 ttgcttgctc cagaaggcta tggagaaatt atcggtgggt ctatgcgtga ggaagattac
     3241 gatgcccttg tcgctaagat ggatgaactt ggcatggaac gtacagaata tgaattctac
     3301 cttgaccttc gtaaatacgg tacagttcca cacggaggat ttggtatcgg tatcgaacgt
     3361 atggtaacct tcgcagcagg aacaaaacat atccgtgaag ctattccatt cccacgtatg
     3421 ttgcaccgta tcaaaccata agaacaggaa aacgcccatg tggcgttttt tcgaattcta
     3481 gtgtttttac ttgcgtaaaa aaattttttc tagtataata gtttattgtg agcgaacctc
     3541 acttacccct tgcaaagtct tggggtcatt agaccaaaag gaggaacata tcaatggcta
     3601 aatacgaaat tctttatatc attcgtccaa acattgaaga agaagctaaa aacgctttgg
     3661 tagcacgttt tgactctatt ttgactgaca acggtgcaac tgttgttgaa tcaaaaactt
     3721 gggaaaaacg tcgtcttgca tacgaaatcc aagatttccg tgaaggactt taccacatcg
     3781 ttaacgttga agcaaatgac gatgcagctc ttaaagagtt tgaccgtctt tcaaaaatca
     3841 acgctgacat tcttcgtcac atgatcgtca aaattgacgc gtaagaaggt aaactatgat
     3901 taacaatgtt gtacttgtag ggcgtatgac acgtgacgct gagttgcgtt ataccccatc
     3961 aaatgtagca gttgcgactt ttactcttgc agtaaaccgt acatttaaga gtcaaaatgg
     4021 tgaacgtgag gctgatttta tcaatgtcgt tatgtggcgc caacaggctg aaaatcttgc
     4081 taactgggct aaaaaaggct cacttatcgg ggtgacaggt cgtatccaga ctcgtagtta
     4141 cgataaccag caaggacaac gtgtctacgt gacagaggtc gtggctgaga atttccaaat
     4201 gttggaaagc cgtagtgtgc gtgagggtca cacaggtgga gcttactctg caccaactgc
     4261 aaactattca gcacctacaa attcagtacc agacttttca cgtaatgaaa atccatttgg
     4321 agcaacaaac ccattggata tttcagatga tgatttacca ttctaatgga caactaaact
     4381 tataaaggag aaaaaacatg gctcaacaac gtcgtggcgg attcaaacgc cgtaaaaaag
     4441 ttgattacat cgcagcaaac aaaattgaat atgttgatta caaagatact gagcttctta
     4501 gccgtttcgt ttcagaacgt gggaaaatcc ttcctcgtcg tgtaacagga acttcagcta
     4561 aaaaccaacg taaagtaaca acagctatca aacgcgctcg cgtaatggct ttgatgcctt
     4621 tcgtaaacga agattaaaga aaagacccgg ttgggtctct ttttcatgag cttgaaaaca
     4681 agaaagcgaa ttaagacccc aacgggtctt ttttcacaag ctttgaaatg agagagcgag
     4741 ttaggctcag tggcttcctt tttacgagtt tgaaaacaag agaacacatt aaaagcacga
     4801 tggagctttt tcataaatat gtctcttttc tcatccaaga aattaaaata aaaatgataa
     4861 actttttgaa aataggggat ttgcctactt tatgatataa tggaaagagt tagattgaaa
     4921 gaggtagtga gatggacgcg aaattaagat acaaggcaaa gaagatcaag attgtctttt
     4981 ttgatattga tgatacattg cggaattcaa agacagggtt tattccaact acaattccca
     5041 ctgtttttaa acagttgcgt gaaaaaggaa ttttaagaag ctatcaatcc aatttatccc
     5101 gacttagatg tagatcccga tttccatgaa aaagaagata tctatcagat gtggactttt
     5161 gaagataagg gagatgactt gcacttgcct gacagtctct cagacaaact tcgcatggtt
     5221 cgttggcatc aacattcgtc tgatattgtg ccgatttcag gctccaaagc gacgggggtg
     5281 gaaaaggttg tggaacacct tggcttgaaa ccagagaagg tcatggtttt tggagatggt
     5341 ctcaatgact tggaactctt tgattatgct ggaatcagcg ttgccatggg aatttctcat
     5401 gataaaatca aagaaaaagc agattatatt acaaaaacat tagaagaaga tggcattttt
     5461 gatgccttag aagtatttgg tatggtagaa aaagaattgc attttccaca agtagacatt
     5521 gaaacagtag aaggtcttct tgcgactatt aagaccaatc acggagactt acgtatcaag
     5581 cttttccctg aacatgctcc taaaacagtg gctaactttg tatctctttc aaaagatggc
     5641 tactatgatg gtgtcatttt ccaccgtatt atcaaggact ttatgatcca aggtggagac
     5701 ccaactggaa ctggtatggg tggcgagtca atctacggcg aatcttttga ggatgaattc
     5761 tcagaagagc tttacaatat ccgtggtgct ctttccatgg caaatgctgg tccaaatacc
     5821 aacggcagcc agttctttat cgtgcaaaac caacacctac cttattcgaa gaaagaaatt
     5881 actcgtggtg gttggccaga accgattgca gaaatctatg ccaatcaagg tgggacacct
     5941 cacctagacc gccgtcacac ggtttttggt cagttagctg atgaagcatc ttacgctgtc
     6001 ttggatgcca ttgctgctgt tgagacagga gctatggaca agccagttga agatgttgta
     6061 attgaaacta ttgaaatcga ggactaagat gaaaatcggt gataagctaa aggggcgtat
     6121 tacagggatt cagccctacg gtgcctttgt tgagttagag acgggtgata cggggctgat
     6181 tcacatttca gaaattcgga caggatttat tgaaaatatt catgaaacct tgaaagtcga
     6241 tgaagaggtt caagttcagg tagtggattt agatgaattt acggggaaag ccagtctttc
     6301 tatccgcact ttggaggaag aaaagtacca gtttcctaga cggagacgct tttcaagtga
     6361 ccgctttaac tacggttttg ctccctttcg acgaatgcta ccaatctgga ctggagaagc
     6421 tctgcaccat ttaaaaaaga agaagtagtt tagagtttta tattttttgt aatgttaata
     6481 taaatttatt ataataggac catggaagaa gaacaattat taaaatcagg agagcgcatt
     6541 aaccagctct tttcgacaga tatcaaaatc attcaaaata gagaggtttt tagctattcg
     6601 gtggatagtg ttctcttatc acgatttcca cgttttccta agaaggggtt gattgtggat
     6661 ttctgtgctg ggaatggagc agtggggctt tttgctagca ctcgtactca agcacagata
     6721 ttgtctgttg agattcagga gcgtttggcg gatatggctg aacgctctgt ccgtttgaat
     6781 ggattagagg agcaaatgca ggtcatctgc gatgatttga aaaatatgcc tgctcacatt
     6841 caggggagta aggtagatat gattttgtgt aatccaccct atttcaaggt ggatccttat
     6901 tctaatctga acgagagtga acattatctc ttggctcgac acgaaatcac gactaatttg
     6961 gaagaaatct gtcgtagtgc ccagagtatt ctcaagtcta atgggcgttt ggccatggtt
     7021 catcgtcctg atcgacttct ggatattttg gatacgttaa aacgacataa tctagcccct
     7081 aagcgcctgc agtttgttta tccaaaaaga gaaaaggaag ccaatatgct tttgattgag
     7141 gcgatcaagg atggctcaac aagtggcttt aaggtcttac ctcctttgat tgtccacaat
     7201 gatgatggct cttatacgcc cgaaatcgaa gagatttatt atggatcata aggcttatat
     7261 gtacgttctg gagtgtcgtg atggatccta ctatataggc tatacgactg atgtgagaag
     7321 acgcctcgct atccacaata gtgggaaggg agccaaatat acacgagcac gcttgccagt
     7381 caaacttatc tatgctcaag gttttgccag taaggaagaa gccatgtcgg ctgaagctct
     7441 tctcaagcgt aagaagaggc cacagaagga agaattttta tctgaaaatc aagatagaaa
     7501 tttactcagt tattttgaag aatcgtgggg agtcctttga ctcctcttac ctttgtcaaa
     7561 aagcgaaaaa aatgctacaa taaagaaaat aaacaaaatg aggtattatc atgtccaaga
     7621 ttctagtatt tggtcaccaa aatccagact cagatgccat cgggtcatct gtagcttttg
     7681 cctaccttgc aaaagaagct tacggtttgg atacggaagc tgttgccctt ggaactccaa
     7741 atgaagaaac agcctttgtc ttgaactatt ttggtgtgga agcaccacgt gttatcactt
     7801 ctgccaaagc agagggggca gagcaagtta tcttgactga ccacaatgaa ttccaacaat
     7861 ctgtatcaga tatcgctgaa gtagaagttt acggtgttgt agaccaccac cgtgtggcta
     7921 actttgaaac tgcaagccca ctttacatgc gtttggagcc agttggatca gcgtcttcaa
     7981 tcgtttaccg tatgttcaaa gaacatggtg tagctgtgcc taaagagatt gcaggtttga
     8041 tgctttcagg tttgatttca gatacccttc ttttgaaatc accaacaaca cacccaacag
     8101 ataaaatcat tgctcctgaa ttggctgaat tggctggtgt gaacttggaa gaatatggtt
     8161 tggcaatgtt gaaagctggt accaacttgg ctagcaaatc tgctgaagaa ttgattgata
     8221 tcgatgctaa gacttttgaa ctcaacggaa ataatgtccg tgttgcccaa gtgaacacag
     8281 ttgacatcgc tgaagttttg gaacgccaag cagaaattga agctgcaatg caagctgcaa
     8341 acgaatcaaa cggctactct gactttgtct tgatgattac agatatcgtc aactcaaact
     8401 cagaaatctt ggctcttggt gccaatatgg acaaggtcga agcggctttc aacttcaaac
     8461 ttgaaaacaa tcatgccttc cttgctggtg ccgtttcacg taagaaacaa gtggtacctc
     8521 aattgactga aagctttaat gcgtaagatt ttgggtgtca gctcaaaatc ggaaagtcta
     8581 gtttgcctta tatcgaaagg agtttcggct ccttttttct aggagtgaag tatgttagaa
     8641 aatggcgatt tgatttttgt gagagatggg tcagacatgg gacaggccat ccagacttcc
     8701 acaggtaact atagccatgt tgccatttat ttggatggga tgatttatca tgctagtgga
     8761 caggctggtg ttgtctgtca agaaccggca gacttctttg agtccaatca tttgtacgac
     8821 ctctatgttt acccagaaat ggatatccag tcggtgaagg aaagagcttg cagacatctt
     8881 ggagcaccct acaatgcttc tttctatcca gatgcagctg gtttctactg ctcccagtat
     8941 atagcagaaa tcctacctat ttttgaaact attcctatga aatttggaga tggggaacag
     9001 gagattagtg atttttggag ggagtattac atagaactag gtctgcctgt tcctctgaac
     9061 caagctggga ccaatcctag tcagttggca gcatcgcctc tgttacaatg taaagaaagg
     9121 aatcttcatg attcagattt ttaatccatc tcgtttgacg agacagccat ttttggagaa
     9181 ttgatccgct atctggacca gtatgaggat gtgattctac gggaaattaa ggctcaattt
     9241 ccagatgttg cagttgataa actcatggaa gagtatataa aggcaagctt gattctacgt
     9301 gaaaataagc gctattacct caattttcct acgcttgaat cacttgatag tcttgaactg
     9361 gatcaagaga tttttgtcag agaagctagt ccggtctatc aagccttgtt ggagcagagt
     9421 tttgagacgg aattgcgcaa tcaaatcaat gcagctattt tagttgaaag acggactttg
     9481 cgcgcattaa aatgaccctg tccaattatt tttacaaggt caaacagcag tatcctttga
     9541 cagaaaaaca gcaggagctc tatgacattt taggagatgt taatcctgag tatgcactca
     9601 agtatatgac ggcttttttg ttgaaatttc tcaaaaaaga ccagcttatg cagaaatgcc
     9661 gtgatatctt tgtggacagt ttggttgtct taggctatat tgtgcaaaat gaagatagaa
     9721 agtatgagtt ggctatcgat tttgataagg agagattaac tttctactta gcgtgatttc
     9781 ttgtttctga gcacattgtt tgactttcct tagtattcgg tataaactat atgtaaccgg
     9841 taacacatat cggaataaac taaaggagac aatcatatgt cacttgaaaa caaattggaa
     9901 caagcaacag gcgctgtcaa agaaggtttt ggtaaagtta ctggagacag caagacagaa
     9961 cttgaaggag ctgttgaaaa aacagttgct aaggcaaaag acgttgtaga agacgcaaaa
    10021 ggtgctgtag aaggtgccgt tgaaggtttg aaaaacgttt ttactaaata ataggaaaaa
    10081 atcaagggtt tcattttccc ttgatttttt ctattcttat aaataatttt ctgcgacggc
    10141 tgtatctcct gggtaggatt ctttcttgcc ctggatgatt tggtaacaat cggctccctt
    10201 acccgcaata ataactgcat ctaattcgtg atttgtgata gccattgccg ccttgatggc
    10261 ttcttggcga tccgcaatct tttcaacagg atgattgatg tagctactaa tttcatctgc
    10321 aatggccatt gggtcttcat agttagggtc atcagcagtc agaaagactt gaatctcagg
    10381 gtgttgattg aggaggaggc caaagtcctt acgacgactt tctcccttgt ttcctgttga
    10441 tcccagaacc agagcaatct ttccggtttg atgagtttca accacattga tgagtttttt
    10501 cagactatcc ccattgtggg catagtcgat gaagaccttg gctccatttt tctgagtgag
    10561 gacttccata cgaccaggaa cgcgggttgc agcgatgcct tttttgatgt cctcaagact
    10621 tgctccgaga cggagacaag caagtccagc agcaactgca ttttcttggt tgaagttgcc
    10681 aatgagttgg atatcataat ctccagcgag tttacccgta gctgaaaagc taaaggcttt
    10741 ggaattctcg atttggttat caaattggct accatagaaa tcatggtctt gatcttcaac
    10801 ctgttctttc aagactgaga agtggtccat gtcactgtta atgatgactg ctcggctatt
    10861 ttccatcaag agacgcttgt ggtagaaata gtcttcaaag ctagagtgtt caatcgggcc
    10921 gatatggtca ggagtgatgt taagaaagac tcctacatca aaggtcagtc catagactcg
    10981 atggactaga taggcttgac tggagacttc catgatgagg tgggtacggt cattttgcac
    11041 agcctgattc atcatgtcaa agaggtcaat actctcaggg gttgtcaacg ctgacttaaa
    11101 aaaagtctcg ccatcaagag ttgtgttcat ggtcgacaac atagcaggtc tatgcccttg
    11161 agataagatg ttataggcga aataggttgc tgttgtctta cccttagtac cagtaaaggc
    11221 aaggagtttg agtttctctt gtggattacc atagaactcc atggcaatca aactcatggc
    11281 tttctttata tcgttcacaa tgatgacagg gatatcgact tcgtagtcct tttcagctac
    11341 ataccaagct aaaccttgtg ttatagcaga aagaaggtat tcttttttaa aggcagcgcc
    11401 ttttgcaaaa aaaagagtgt cttctgttac ttttcggctg tcgtagctga tgctatcaaa
    11461 aataactttg ctgtagttgt agtggtaatg accttggtca ataatttcgc gaaaaaggcc
    11521 atctttcttt aaaatatcta atacggtttc aatcttaatc atactttcta ttgtaaaccg
    11581 aaagtcgtaa atttacaagt aacaaggaaa agtttataat ggaagataag gagtttttcc
    11641 tagttatcaa aattgaatga ggaatctatg tcgcacgaaa acaatcacca gcaggcccag
    11701 atgttacggg ggactgcttg gctaacggct agtaacttta tcagtcgcct actcggggct
    11761 gtttacatta tcccttggta catctggatg ggggcttatg cagctaaggc aaatggtctc
    11821 tttaccatgg gttacaatat ctatgcttgg ttcttgttgg tttcaacagc ggggattcca
    11881 gttgcggtgg ccaagcaagt tgccaagtat aataccatgc gagaagaaga gcatagcttt
    11941 gccctgattc ggagcttctt aggctttatg acaggactag gtctggtttt tgctttagtc
    12001 ttgtatgtct ttgctccttg gctagcagac ttgtctggcg tgggcaaaga cttgatccca
    12061 atcatgcaaa gcttggcttg gggagtcttg attttcccgt ctatgagtgt tatccgagga
    12121 tttttccaag ggatgaataa cctcaaaccc tatgctatga gccaaattgc tgagcaggtc
    12181 attcgtgtta tctggatgct cctagcaacc tttatcatta tgaagctcgg ttcaggagat
    12241 tatctagcag ccgttaccca atcaaccttt gctgcctttg tcggtatggt agccagtttt
    12301 gcagtcttga tttatttcct tgcccaagaa ggttcactca aaagagtctt tgaaacagga
    12361 gataagatta acagtaagcg tctcttggtt gataccatta aggaagccat tccttttatc
    12421 ctgacagggt ctgccatcca gctcttccag attttggatc agctgacctt tatcaatagt
    12481 atgagctggt ttaccaacta cagcaatgag gacttggttg tcatgttttc ttatttctca
    12541 gccaatccta ataaaatcac gatgattttg atttctgtag gggtttcgat tgggagtgtt
    12601 ggtttgccac ttttgacgga aaactatgtc aagggggact tgaaagcagc ttctcgtctc
    12661 gttcaggaca gtctcaccct actctttatg ttcttgctac cagcaacggt tggagtggtt
    12721 atggtaggag aacctcttta tacggtcttc tatggtaagc cagatagttt ggctctggtc
    12781 ttatttgtct ttgcagtttt gcagtctatt attttaggct tgtacatggt cttgtctcca
    12841 atgcttcagg ccatgttccg caaccgcaag gccgttctct attttatcta tggttctatt
    12901 gccaagctag tcttgcaact acctaccatc gccctcttcc acagttatgg tcctttgatt
    12961 tcaacaacca ttgctctcat cattcctaac gtcttgatgt atcgggatat ttgtaaagta
    13021 actggtgtca agcgcaaggt gattttgaag cgaaccattt taatcagttt gctgacccta
    13081 gtcatgtttc tgttaatagg aaccatccag tggctgttag gatttttctt ccaaccaagt
    13141 ggacgtttgt ggagcttctt ttatgtagct cttgtcggtg ccatgggggg tggactttat
    13201 atggttatga gtctgcgtac ctatttatta gataaggtaa taggaaaagc ccaagcagat
    13261 cgcctgcgag caaaatttaa gctttcgtaa ggtagcattt ataaataaaa ggaataagtt
    13321 gaacaatctt ttaggagaac ttgaaactta ttcctttttt agcgtcaaaa tagctaaaat
    13381 tatctttcaa aaatagaaaa tgaggttttt ttgatttttt ggaaaaaaca attgactaaa
    13441 caaacccaga gttgtataat aaaacaaata tttaaagcag gaggttctgg aaatgaaaaa
    13501 gtctaagagc aaatatctaa ccttggcagg tcttgtcctg ggtacaggag ttttattgag
    13561 cgcgtgtgga aattctagca ctgcatcaaa aacctataac tatgtttatt caagtgatcc
    13621 atctagtttg aactatctag cagaaaaccg cgcagcaaca tccgatattg ttgcaaattt
    13681 ggtagacggg ttattagaaa atgaccaata tgggaatatt attccatcat tagcagagga
    13741 ttggactgtt tctcaggacg gtttgaccta tacctacaaa cttcgtaagg atgccaagtg
    13801 gtttacttct gagggagaag aatatgcgcc tgtaactgcc caggattttg tgacaggttt
    13861 gcaatatgca gctgataaaa aatcagaagc tttgtatcta gtgcaggact ctgttgctgg
    13921 tttggatgac tatatcactg gtaaaacaag cgacttttca actgtcggtg tcaaggcact
    13981 tgatgaccaa acggttcaat atactttggt taaaccagaa ctttactgga attcaaaaac
    14041 acttgcaacg atactttttc ctgttaatgc agatttcctg aaatcaaaag gggatgattt
    14101 tgggaaggcg gatccatcta gtattttgta caatggacct ttcttgatga aagcacttgt
    14161 ctcaaaatct gctattgaat ataagaaaaa ccctaattac tgggatgcta agaatgtctt
    14221 tgtagacgat gtgaaattga cctactatga tggtagcgac caagaatcac tggaacgtaa
    14281 ttttacagct ggtgcttata ctacggctcg tctttttcct aacagctcca gctatgaagg
    14341 gattaaagaa aaatacaaaa acaatatcat atatagtatg caaaattcaa cttcatattt
    14401 ctttaatttt aacctagata ggaagtctta caattatact tctaaaacaa gtgacattga
    14461 aaagaaatcg actcaggaag cagttctcaa taaaaacttc cgtcaggcta tcaattttgc
    14521 ttttgacaga acatcttatg gggctcagtc tgaagggaaa gaaggtgcaa caaagatttt
    14581 gcgtaaccta gtggttcctc caaactttgt cagtatcaag ggaaaagact ttggtgaagt
    14641 tgtagtctct aagatggtca actatggtaa ggaatggcaa ggtatcaact ttgcggatgg
    14701 tcaagaccct tactacaatc ctgagaaagc caaggctaag tttgcggaag ctaagaaaga
    14761 actcgaagca aagggtgttc aattcccaat ccacttggat aagactgtgg aagtaacaga
    14821 taaagtaggc atacaaggag ttagttctat caaacaatca attgaatctg ttttaggttc
    14881 tgataatgta gtgattgaca ttcagcaatt aacatcagat gagtttgaca gttcaggcta
    14941 ctttgctcaa acagctgctc agaaagacta tgatttatat catggcggtt ggggacctga
    15001 ttatcaagac ccgtcaacct atctcgatat ttttaatact aatagtggag gagttctaca
    15061 aaatcttgga ctagagcctg gagaagccaa tgacaaggct aaggaagttg gactggatgt
    15121 ctatactcaa atgttggaag aagctaataa agagcaagat ccagccaaac gttatgagaa
    15181 atatgctgat attcaagctt ggttgattga tagttcttta gttcttccaa gtgtttcgcg
    15241 tgggggaaca ccatcattga gaagaaccgt accatttgct gctgcctatg gtttaaccgg
    15301 tacaaaaggg attgaatcat ataaatacct caaagtacaa gataagattg tcacaacaga
    15361 cgaatatgca aaagctagag aaaaatggtt gaaagaaaaa gaagaatcca ataaaaaagc
    15421 ccaagaagaa ttggcaaaac atgtcaaata aaatctaatt gtagataagt ttgtgtaaga
    15481 agtctttgtg cttaaaaacg cataatatca ggtatataaa atcctagatg ttatgcgttt
    15541 tgttatagga agatttatta gattttatcc tcaaattgag ttattcccga ctttatccgt
    15601 tgaaatatta taaggagtat ttatgaaatt aaaattatta agagtagata ctaaggtgat
    15661 tatggggagt ttcttacttg ttctgtctag tctacttgct ttgttgcttc cccttatctt
    15721 aaagggttta atagatggga gttctattga aaatataggt tccaaagtat ttcaatcgtt
    15781 tttgattttt attggtcaag ccttgttttc ttctattggt tactatctgt ttagtcaatc
    15841 gggtgaaaaa aagatagcaa aaatcaggaa aaaagtgata gaggggttga tttatgcaga
    15901 gaaatccttc tttgataaga gccaaagtgg ggagttgact tctgccattg tcaatgacac
    15961 gagtgtcatt cgtgagtttt taattacgac tttcccaaat attattctga gtttagttat
    16021 ggtacttggt tccattgtag tcttatttag tcttgattgg aatctttctc tacttttatt
    16081 catcactctt ccttgtatga tgtttattat cttgctcctt tccaatatca gtgaaaagta
    16141 tagtcgtcgt ttacaggagg aaatcggatt tttaacaggg taattgactg aaaagattca
    16201 agaacatgaa ttaattaaga ctaaccaagc agagaaaagt gtacaggatg ttttggataa
    16261 ttgtattgaa agggtacaaa acaattcact gaaatcagat agggttactt cttttgagac
    16321 cccgtttgct ctcttattta tctttgcgac tatagctgtg atgctaacct atgggggtta
    16381 tcgggtcagc gcaggatata tatctgtggg aaccttggtt tcgtttttga tttacctctt
    16441 tcaattactt aatcctatta gtaatatagc taattttgta actgtttatt ctaggagcaa
    16501 gggatcttca gttgcactgg agaacttgct tgcagttcct aaagaaaaat ttgagtgagg
    16561 aaaatcggta tcaggacaag ggttgaattt
//
LOCUS       DAVBNO010000042        16459 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVBNO010000042 DAVBNO010000000
VERSION     DAVBNO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.042"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..906
                     /gene="lytB"
                     /locus_tag="VQ846_001514"
     CDS             <1..906
                     /gene="lytB"
                     /locus_tag="VQ846_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HET3076073.1"
                     /translation="YMAKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDS
                     DGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYES
                     DGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNY
                     SAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKD
                     KNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEY
                     ASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(967..2622)
                     /gene="pavA"
                     /locus_tag="VQ846_001515"
     CDS             complement(967..2622)
                     /gene="pavA"
                     /locus_tag="VQ846_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET3076074.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            2727..3224
                     /gene="ybeY"
                     /locus_tag="VQ846_001516"
     CDS             2727..3224
                     /gene="ybeY"
                     /locus_tag="VQ846_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET3076075.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            3205..3600
                     /locus_tag="VQ846_001517"
     CDS             3205..3600
                     /locus_tag="VQ846_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET3076076.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            3617..4516
                     /gene="era"
                     /locus_tag="VQ846_001518"
     CDS             3617..4516
                     /gene="era"
                     /locus_tag="VQ846_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET3076077.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            4565..5389
                     /gene="mutM"
                     /locus_tag="VQ846_001519"
     CDS             4565..5389
                     /gene="mutM"
                     /locus_tag="VQ846_001519"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET3076078.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYHKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFCLTDKVLISHLRMEGKYFYYPDQVPERKHAHVFFRFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            5389..5994
                     /locus_tag="VQ846_001520"
     CDS             5389..5994
                     /locus_tag="VQ846_001520"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET3076079.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            5981..7180
                     /locus_tag="VQ846_001521"
     CDS             5981..7180
                     /locus_tag="VQ846_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HET3076080.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            7180..7329
                     /gene="rpmG"
                     /locus_tag="VQ846_001522"
     CDS             7180..7329
                     /gene="rpmG"
                     /locus_tag="VQ846_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET3076081.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            7369..7602
                     /gene="secG"
                     /locus_tag="VQ846_001523"
     CDS             7369..7602
                     /gene="secG"
                     /locus_tag="VQ846_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET3076082.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            7704..10058
                     /gene="rnr"
                     /locus_tag="VQ846_001524"
     CDS             7704..10058
                     /gene="rnr"
                     /locus_tag="VQ846_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET3076083.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLKLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     AGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKKYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGESYADVLSMMLLRSMQQARYSEHNYGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            10021..10488
                     /gene="smpB"
                     /locus_tag="VQ846_001525"
     CDS             10021..10488
                     /gene="smpB"
                     /locus_tag="VQ846_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET3076084.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKRQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            10504..11364
                     /gene="tehB"
                     /locus_tag="VQ846_001526"
     CDS             10504..11364
                     /gene="tehB"
                     /locus_tag="VQ846_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET3076085.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMETVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            11437..12390
                     /gene="coiA"
                     /locus_tag="VQ846_001527"
     CDS             11437..12390
                     /gene="coiA"
                     /locus_tag="VQ846_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET3076086.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            12409..14211
                     /gene="pepF"
                     /locus_tag="VQ846_001528"
     CDS             12409..14211
                     /gene="pepF"
                     /locus_tag="VQ846_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET3076087.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            14213..14926
                     /locus_tag="VQ846_001529"
     CDS             14213..14926
                     /locus_tag="VQ846_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET3076088.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            14993..15934
                     /gene="prsA"
                     /locus_tag="VQ846_001530"
     CDS             14993..15934
                     /gene="prsA"
                     /locus_tag="VQ846_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET3076089.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDIITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(16051..16392)
                     /locus_tag="VQ846_001531"
                     /pseudo
     CDS             complement(16051..16392)
                     /locus_tag="VQ846_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4952 a         3075 c         3687 g         4745 t
ORIGIN      
        1 tacatggcga aaaatgagac agtagatggt tatcagcttg gaagcgatgg taaatggctt
       61 ggaggaaaag ctacaaatga aaatgctgct tactatcaag tagtgcctgt tacagccaat
      121 gtttatgatt cagatggtga aaagctttcc tatatatcgc aaggtagtgt cgtatggcta
      181 gataaggata gaaaaagtga tgacaagcgc ttggctatta ctatttctgg tttgtcaggc
      241 tatatgaaaa cagaagattt acaagcgcta gatgctagta aggactttat cccttattat
      301 gagagtgatg gccaccgttt ttatcactat gtggctcaga atgctagtat cccagtagct
      361 tctcatcttt ctgatatgga agtaggcaag aaatattatt cggcagatgg cctgcatttt
      421 gatggtttta agcttgagaa tcccttcctt ttcaaagatt taacagaggc tacaaactac
      481 agtgctgaag aattggataa ggtatttagt ttgctaaaca ttaacaatag ccttttggag
      541 aacaagggcg ctacttttaa ggaagccgaa gaacattacc atatcaatgc tctttatctc
      601 cttgcccata gtgccctaga aagtaactgg ggaagaagta aaattgccaa agataagaat
      661 aatttctttg gcattacagc ctatgatacg accccttacc tttctgctaa gacatttgat
      721 gatgtggata agggaatttt aggtgcaacc aagtggatta aggaaaatta tatcgatagg
      781 ggaagaactt tccttggaaa caaggcttct ggtatgaatg tggaatatgc ttcagaccct
      841 tattggggcg aaaaaattgc tagtgtgatg atgaaaatca atgagaagct aggtggcaaa
      901 gattagtact ataagtgaat atgatttgag tgaatagtaa gttaaaaatc ctgatttcaa
      961 gtaaaatcag gattttttca tggatgcaat ttttttggag tctggtgtga cgcggagggt
     1021 cttttgtcct gtgtaagtga caaagccggg ttttccacca gttggtttat tgagtttttt
     1081 gacttcaatc atatctacct gcaccagatt cgacaggcgc ccttgagaga agtaggcagc
     1141 taactctgct gcgtctgtct tgactgcatc agatgggtca agatttcctg agatgacaac
     1201 atggcttcca ggaatgtcct tagcatggaa ccaaagttcc tccttgcggg ccattttaaa
     1261 ggtcaattcc tcattttgaa gattgtttcg tccgacatag atgatggttt tgccatcgct
     1321 tgctagatat tgttctagtt ttttgcgttt ctggattttc tcccgttgtc ttctgcggat
     1381 aaaacctgtt tgaatcaatt cttcacggat ttcagcgatt tcttccagtc cagcttggtt
     1441 gaggacggtt tctacacttt ccagatagag aatagtggct ttggtttctt caatcaaatc
     1501 agtcaagtat ttgacagctt ctttgagttt ctgataccgt ttaaaatagc gttgggcatt
     1561 ctggttggga gtcagagcct tatcaagcgc aatcatgata ggttggttgg tatagtagtt
     1621 gtctaggata acctggtctt ggtcgttagg cacttggtgg aggaaggttg tcagcaattc
     1681 tcctttttga cgaaattctt cagcgttgtc tgtcgccagt aactcttttt cctgtttttt
     1741 gagtttgtgt cggtttttct gaagttcatt ttcaacacga cgaatcagtt cactggcctg
     1801 ctgtttgacg cggtcgcgct cagccttatc cttatagtag gtgtccaaca aatcagaaag
     1861 atttgcaaga ggctctccca cctgatttgc aaaaggaact ggactgaagg aagtctcagt
     1921 caagcatggc ttggtttctt gattgaaaaa atttcggaaa gcggaaagtt tttcactaac
     1981 cagtatcctt tccaattcat ttgccgtatc gcgtcccaga ccttgaaaga ggctttgaag
     2041 attttttgct gttagttctt gggtttgcag gatttcaaag agcttttcat ccttgatagt
     2101 aaaaggattg agagattttg tacttggcgg agcgatatag gtcgatcctg gaagtaaggt
     2161 gcggtagcta ttttgtgaaa agccgacgtg tttgataact tcgaggattt tatgactgct
     2221 tttatcgacc agtagaatat tactgtgttt ccccataatt tcgataatca aggtagcctg
     2281 gatatggtct ccaatctcgt ttttattgga aactgtaatt tccacaatac ggtcattttc
     2341 cacttgctca atcgactcaa tcagggcccc ctgcaaatac tttctcaaaa ccatgataaa
     2401 ggtagaaggt tgggctggat tttcaaaagt cgtttgggtc agctgaatgc gtccaaaaac
     2461 tggatgggca gaaaggagca ggcgatggct ttggcgattg ctgcggattt gcaagaccaa
     2521 ctcttgttca aaaggctgat tgattttctg gatgcgacca ttcactaatt cgcttcgcaa
     2581 ttcctcaact atgtggtgta aaaaaaatcc gtcaaatgac atcgttctct ccttgtgatt
     2641 gtattccata gtattatatc aaaaaggtag aataaaatca tggaaatgtg gtataataaa
     2701 gccaagtaaa gagaaacgag aagcacatgt atattgaaat ggtagatgaa actggtcaag
     2761 tttcaaaaga aatgttgcaa caaacccaag aaattttgga atttgcagcc caaaaattag
     2821 gaaaagaaga caaggagatg gcagtcactt ttgtgaccaa tgagcgtagt catgaactta
     2881 atctggagta ccgtgacacc gaccgtccga cagatgtcat cagccttgag tataaaccag
     2941 aattggaaat tgcctttgac gaagaggatt tgcttgaaaa tccagaattg gcagagatga
     3001 tgtctgagtt tgatgcctat attggggaat tgttcatctc tatcgataag gctcatgagc
     3061 aggccgaaga atatggtcac agctttgagc gtgagatggg cttcttggca gtacacggct
     3121 ttttacatat taacggctat gatcactaca ctccggaaga agaagcggag atgttcggtt
     3181 tacaagaaga aattttgaca gcctatggac tcacaagaca ataaacgaaa atggaaaaat
     3241 cgtgacttga tatccagttt agaatttgct ttgacaggta tttttactgc tatcaaggaa
     3301 gaacgcaata tgcgaaaaca cgcagtgacg gctctagtgg tcatccttgc aggttttgtt
     3361 tttcaggtgt cacgaatcga atggctcttt ctcctattga gtattttctt ggtagtagcc
     3421 tttgagatta tcaactctgc tattgaaaat gtggtggatt tggccagtca ctatcacttt
     3481 tccatgctgg ctaaaaatgc caaggatatg gcggctggcg cggtattagt ggtttctctt
     3541 ttcgcagcct taacaggcgc attgattttt ctcccacgaa tctgggattt attattttaa
     3601 acagtaagag gaaattatga cttttaaatc aggctttgta gccattttag gacgtcccaa
     3661 tgttgggaag tcaacctttt taaatcacgt tatggggcaa aagattgcca tcatgagtga
     3721 caaggcgcag acaacgcgca ataaaatcat gggaatttac acgactgata aggagcaaat
     3781 tgtctttatc gacacaccag ggattcacaa acctaaaaca gctctcggag atttcatggt
     3841 tgagtctgcc tacagtaccc ttcgcgaagt ggacaccgtt cttttcatgg tgcctgctga
     3901 tgaagcgcgt ggtaaggggg acgatatgat tatcgagcgt ctcaaggctg ccaaggttcc
     3961 tgtgattttg gtggtgaata aaatcgataa ggtccatcca gaccagctct tgtctcagat
     4021 tgatgacttc cgtaatcaaa tggactttaa ggaaattgtt ccaatctcag cccttcaggg
     4081 aaataacgtg tctcgtctag tggatatttt gagtgaaaat ctggatgaag gtttccaata
     4141 tttcccgtct gatcaaatca cagaccatcc agaacgtttc ttagtttcag aaatggttcg
     4201 cgagaaagtc ttgcacctaa ctcgtgaaga gattccgcat tctgtagcag tagttgttga
     4261 ctctatgaaa cgagacgaag agacagacaa ggttcacatc cgtgcaacca tcatggtcga
     4321 gcgcgatagc caaaaaggga ttatcatcgg taaaggtggc gctatgctta agaaaatcgg
     4381 tagcatggcc cgtcgtgata tcgaactcat gctaggagac aaggtcttcc tagaaacctg
     4441 ggtcaaggtc aagaaaaact ggcgcgataa aaagctagat ttggctgact ttggctataa
     4501 tgaaagagaa tactaagtag aggtaggctc atgcctgctt cttgttttta cagaaggagg
     4561 acttatgcct gaattacctg aggttgaaac cgtttgtcgt ggcttagaaa aattgattat
     4621 aggaaagaag atttcgagta tagaaattcg ctaccacaag atgattaaga cggatttgga
     4681 agagtttcaa agggaattgc ctagtcagat tatcgagtca atgggacgtc gtggaaaata
     4741 tttgcttttc tgcctgacag acaaggtctt gatttcccat ttgcggatgg agggcaagta
     4801 tttttattat ccagaccaag tgcctgaacg caagcatgcc catgttttct tccggtttga
     4861 agatgggggc acgcttgttt atgaggatgt acgcaagttt ggaaccatgg aactcttggt
     4921 gcctgacctt ttagacgcct actttatttc taaaaaatta ggtcctgaac caagcgaaca
     4981 agactttgat ttacaggtct ttcaagctgc ccttgccaag tccaaaaagc ctatcaaatc
     5041 ccatctccta gaccagacct tggtagctgg acttggcaat atctatgtgg atgaggttct
     5101 ctggcgagct caggttcatc cagctagacc ttcccagact ttgacagcag aagaagcgac
     5161 tgccattcat gaccagacca ttgctgtttt gggccaggct gttgaaaaag gcggttccac
     5221 cattcggacc tataccaatg cctttgggga agatggaagc atgcaggatt ttcatcaggt
     5281 ctatgataag actggtcaag aatgtgtacg ctgtggtacc atcattgaga aaattcaact
     5341 aggcggacga ggcacccact tttgtccaaa ctgtcaaagg agggactgat gggaaaaatc
     5401 atcggaatca ctgggggaat tgcctcaggt aagtcaactg tgacaaattt tctaagacag
     5461 caaggctttc aagcagtgga tgccgacgca gtcgtccacc aactacagaa acctggtggt
     5521 cgtctgtttg aggctctagt acagcacttt gggcaagaaa tcattcttga aaacggagaa
     5581 ctcaatcgcc ctctcctagc tagtctcatc ttttcaaatc ctgaagagca aaaatggtct
     5641 aatcaaattc aaggggagat tatccgtgag gaactggcta ctttgagaga acagttggct
     5701 cagacagaag agattttctt catggatatt cccctacttt ttgagcagga ctacagcgat
     5761 tggtttgctg agacttggtt ggtctatgtg gaccgagatg cccaagtaga acgcttaatg
     5821 aaaagggacc agttgtccaa agatgaagct gagtctcgtc tggcagccca gtggccttta
     5881 gaaaaaaaga aagatttggc cagccaggtt cttgataata atggcaatca gaaccaactt
     5941 cttaatcaag tgcatatcct tcttgaggga ggtaggcaag atgacagaga ttaactggaa
     6001 ggataatctg cgtattgcct ggtttggtaa ttttctgaca ggagccagta tttctttggt
     6061 tgtacctttt atgcccatct tcgtggaaaa tctaggtgta gggagtcagc aagtcgcttt
     6121 ttatgcaggc ttagcaattt ctgtctctgc tatttccgcg gcgctctttt ctcctatttg
     6181 gggtattctt gctgacaaat acggccgaaa acccatgatg attcgggcag gtcttgctat
     6241 gactatcact atgggaggct tggcctttgt cccaaatatc tattggttaa tctttcttcg
     6301 tttactaaac ggtgtatttg caggttttgt tcctaatgca acggcactga tagccagtca
     6361 ggttccaaag gagaaatcag gctctgcctt aggtactttg tctacaggcg tagttgcagg
     6421 cactctaact ggtcccttta ttggtggctt tatcgcagaa ttatttggca ttcgtacagt
     6481 tttcttactg gttggtagtt ttctattttt agctgctatt ttgactattt gctttatcaa
     6541 ggaagatttt caaccagtag ccaaggaaaa ggctattcca acaaaggaat tatttacctc
     6601 ggttaaatat ccctatcttt tgctcaatct ctttttaacc agttttgtca tccaattttc
     6661 agctcaatcg attggcccta ttttggctct ttatgtacgc gacttagggc agacagagaa
     6721 tcttcttttt gtctctggtt tgattgtgtc cagtatgggc ttttccagca tgatgagtgc
     6781 aggagtcatg ggcaagctag gtgacaaggt gggcaatcat cgtctcttgg ttgtcgccca
     6841 gttttattca gtcatcatct atctcctctg tgccaatgcc tctagccccc ttcaactagg
     6901 actctatcgt ttcctctttg gattgggaac cggtgccttg attcccgggg ttaatgccct
     6961 actcagcaaa atgactccca aagccggcat ttcgagggtc tttgccttca atcaggtatt
     7021 cttttatcta ggaggtgttg ttggtcccat ggcaggttct gcagtagcag gtcaatttgg
     7081 ctaccatgct gtcttttatg cgacgagcct ttgtgttgcc tttagttgtc tctttaacct
     7141 gattcaattt cgaacattat taaaagtaaa ggaaatctag tgcgagtaaa aattaatctc
     7201 aaatgctcct cttgtggcag tatcaattac ctaaccagta aaaattcaaa aacccatcca
     7261 gacaagattg aggttttaaa gtattgtccc aaggaaagaa aagtaaccct acatcttgaa
     7321 tctaagtaga ttttatggta aaataatagg gattttaagg agtttgatat gtataaccta
     7381 ttattaacca ttttattagt attatctgtt gtgattgtga ttgcaatttt catgcaacca
     7441 accaaaaacc aatccagcaa tgtatttgat gccagttcag gtgatttgtt tgaacgcagt
     7501 aaagctcgcg gttttgaagc tgtaatgcag cgtttgacag ggattttagt ctttttctgg
     7561 ctagccattg ccttagcatt gacggtatta tcaagtagat aagaaaataa tgggcaggac
     7621 taggtctttg cctcttttta tttttaaagg atgtttgaga aggttttaca gtaaaagaaa
     7681 attaaaaaat ctagaaagaa aatatgaaag atagaataaa agaatattta caagacaagg
     7741 gaaaggtgac tgttaatgat ttggctcagg ctttgggaaa agacagttcc aaggattttc
     7801 gtgagttgat taaaaccttg tccttaatgg aaagaaagca ccaaattcgt tttgaagaag
     7861 atggtagtct gaaattagaa attaagaaaa aacatgagat taccctcaag gggatttttc
     7921 atgcccataa aaatggcttt ggctttgtta gtctggaagg cgaggaggac gacctttttg
     7981 tagggaaaaa tgatgtcaac tatgctattg ctggtgatac cgtcgaggta gtgattaaga
     8041 aagtcgctga ccgcaataag ggaacagcag cagaagccaa aattattgat atcctagaac
     8101 acagtttgac aacagttgtc gggcaaatcg ttctggatca ggaaaaacct aagtatgctg
     8161 gctatattcg ttcaaaaaat cagaaaatca gtcaaccgat ttatgttaag aaaccagccc
     8221 taaaattaga aggaacagaa gttctcaaag tctttatcga taaataccca agcaagaaac
     8281 atgatttctt tgtcgcgagt gttctcgatg tggtgggaca ctcaacggat gtcggaattg
     8341 atgttcttga ggttttggaa tcaatggaca ttgtatccga gtttccagaa gctgttgtta
     8401 aggaagcaga aagtgtgcct gatgctccgt ctcaaaagga tatggaaggt cgtctggatc
     8461 taagagatga aattaccttt accattgacg gtgcggatgc caaggacttg gacgatgcag
     8521 tgcatatcaa ggctctgaaa aatggcaatc tggagcttgg ggttcacatc gcagatgttt
     8581 cttattatgt gaccgagggg tctgcccttg acaaggaagc ccttaaccgt gcgacttctg
     8641 tttacgtgac agaccgagtg gtgccaatgc ttccagaacg actatcaaat ggcatctgct
     8701 ctctcaatcc ccaagttgac cgcctgaccc agtctgctat tatggagatt gataaacatg
     8761 gtcgtgtggt caactatacc attacacaaa cagttatcaa gaccagtttt cgtatgacct
     8821 atagcgatgt caatgatatc ctagctggcg atgaagaaaa gagaaaaaaa tatcataaaa
     8881 ttgtaccaag tatcgaactc atggccaagc ttcatgaaac tttagaaaac atgcgtgtga
     8941 aacgtggagc tctcaatttt gataccaatg aagcgaagat tttagtggat aaacaaggta
     9001 agcctgttga tatcgttctt cggcagcgtg gtattgccga gcggatgatt gagtctttta
     9061 tgttgatggc taatgaaaca gttgccgaac atttcagcaa gttggatttg ccttttatct
     9121 atcgaattca cgaggagcct aaggctgaaa aggttcagaa gtttattgat tatgcttcga
     9181 gttttggctt gcgcatttat gggactgcca gtgagattag tcaggaggca cttcaagaca
     9241 tcatgcgtgc tgttgaggga gaatcttatg cagatgtatt gtccatgatg cttcttcgct
     9301 ctatgcagca ggctcgttat tcggagcaca attacggcca ctatggacta gctgctgact
     9361 attatactca ctttaccagt ccaattcgtc gttatccaga ccttcttgtt caccgtatga
     9421 ttcgggatta cggccgttct aaggaaatag cagagcattt tgaacaagtg attccagaga
     9481 ttgcgaccca gtcttccaac cgtgaacgtc gtgccataga agctgagcgt gaagtcgaag
     9541 ccatgaaaaa ggctgagtat atggaagaat acgtgggtga agagtatgat gcagttgtat
     9601 caagtattgt caaattcggt ctctttgtcg aattgccaaa cacagttgaa ggcttgattc
     9661 acatcactaa tctacctgaa ttttatcatt tcaatgagcg tgatttgact cttcgtggag
     9721 aaaaatcagg tatcactttc cgagtgggtc agcagatccg tatccgtgtt gaaagagcgg
     9781 ataaaatgac tggagagatt gatttttcat tcgtacctag tgagtttgat gtgattgaaa
     9841 aaggcttgaa acagtctagt cgtagtggca gagggcgtgg ttcaaatcgt cgttcggata
     9901 agaaggaaga caagagaaaa tcaggacgct caaatgataa gcgtaagcat tcacaaaaag
     9961 acaagaagaa aaaaggaaag aaaccttttt acaaggaagt agctaagaaa ggagccaagc
    10021 atggcaaagg gcgagggaaa ggtcgtcgca caaaataaaa aggcacgcca cgactataca
    10081 atcgtagata cgctagaggc agggatggtc ctgactggaa ctgaaatcaa gagtgtacga
    10141 gctgctcgaa ttaatctcaa ggatggcttt gctcaagtga aaaatggaga agtttggctg
    10201 agcaatgttc atatcgcgcc ttacgaagag ggcaatatct ggaaccagga accagaacgt
    10261 cgtcgtaaac tcctgctcca taaaaggcaa attcaaaaat tggaacaaga gaccaaaggg
    10321 acaggaatga ccttagttcc ccttaaggtc tatataaaag atggctacgc taagcttctt
    10381 ttaggacttg ccaaagggaa gcatgactat gacaaacggg agtctatcaa acgtcgtgag
    10441 caaaatcgag atatcgcgcg tgtgatgaaa gctgttaatc agcgataaaa agaggaattg
    10501 aaaatggaaa aattagttgc ctataaacgc atgcctttgt ggaataaaca aacaatgcct
    10561 gaagctgttc agcaaaagca caatacaaaa gttgggactt gggggaaaat tactgtcttg
    10621 aagggagctc tcaagtttat tgaattgaca gaagaagggg aagttctagc tgaacacctc
    10681 tttgaagcag gggcagacaa tccaatggcc caacctcaag cctggcaccg agtggaagct
    10741 gccacagatg atgtggaatg gtacttggaa ttttattgta aacctgagga ttattttgct
    10801 aaaaaataca ataccaatcc tgttcattca gaggtcctag aggccatgga gacagtgaaa
    10861 caagggaaag ctttggattt gggttgtggt caggggcgta attctctttt tctagcccag
    10921 caagattttg atgtgacggc tgtagatcaa aatggactag ctcttgaaat cttgcaaagc
    10981 attgtggagc aggaagattt ggacatgcct gttggccttt acgatatcaa ttcagctagc
    11041 attgaacaag aatatgattt tatcgtttca acagttgttc tcatgtttct acaagcggac
    11101 cgcattccag ctattattca aaatatgcag gagaaaacca gtgttggtgg ttacaacctt
    11161 atcgtttgtg ccatggacac ggaggattat ccttgctcgg ttaacttccc attcaccttt
    11221 aaagaaggag aactggcaga ctattacaag gattgggaat tggttaagta caatgaaaat
    11281 ccaggccatt tgcaccgtcg cgatgagaat ggcaatcgta ttcaactacg ctttgcgacc
    11341 ttactagcta agaaaatcaa gtaaacacac atgaagatta ggaattttcc tgatcttttt
    11401 tcttttttac gaatgatata gaaaaggagg gaattcatgt ttgttgcgag agatgctagg
    11461 ggagaattgg taaatgtgtt agaggataaa cttgagaagc aagcatacac ctgcccagct
    11521 tgtggaggcc agctccattt gcgtcaagga ccaagtgtac ggacgcattt tgcccataaa
    11581 tccttaaaag actgtgattt tttctttgaa aatgaaagtc cagaacacct ggccaataag
    11641 gaatccctct atcactggtt gaaaaaagag acaaaggttc aattagagta cccgctttca
    11701 gaacttaaac agattgcgga tgtatttgta aatggcaatc tagctctaga agttcagtgt
    11761 agtcccttgc ctcagaaagt ccttaaagag cgaagtgagg gatatcgtag tcagggttac
    11821 caagtactgt ggttgctggg tcaaaaactg tggctcaagg agcgtttgac tcgtctacag
    11881 caaggttttc tttatttcag tcaaaacatg ggcttttatg tttgggaatt agacaaggaa
    11941 aaacaagttt taagactcaa atacctgatt taccaggatc tccgcggtaa actccattat
    12001 caaatcaagg aattttccta tggtcaaggt agtttattgg aaatattgcg tcttccctat
    12061 aagagacaaa aaatatctca ttttacagtt tctgaggaca aggacatctg tcgctatatc
    12121 cggcaacaac tttattatca aaatctcttt tggatgaaag aacaagcaga agcctatcaa
    12181 aagggagaaa atatcctgac ttatggactg aaagaatggt atccacaaat tcgaccaata
    12241 gtgggcaaat ttttccagat tgaacaagac ttgactagct attatcagca cttttatacc
    12301 tattaccaaa aaaatcctca aaatgattgg caaaagcttt atccaccagc cttttatcag
    12361 caatatttct tgaaaaatat ggtagaatag aaaggatgga ggaatctaat ggtattacaa
    12421 agaaatgaaa taaatgaaaa agatacatgg gatctatcaa cgatctaccc aactgaccag
    12481 gcttgggaag aagccttaaa agatttaaca gaacaattgg agacagtagc ccagtatgaa
    12541 ggccatctct tggatagtgc ggataaccta ctagaaatca ctgaattttc tcttgaaatg
    12601 gaacgccaga tagagaagct ttacgtttat gctcatatga agaatgacca ggatacacgt
    12661 gaagctaagt atcaagagta ctatgccaag gccatgacac tctacagcca gttagaccaa
    12721 gccttttcat tctatgagcc tgaatttatg gagattagcg aaaagcagta tgctgacttt
    12781 ttagaagctc aaccaaagct gcaggtttat caacactatt ttgacaagct cttgcaaggc
    12841 aaggatcacg ttctctcaca acgtgaagaa gaattattgg ctggagctgg agaaatcttt
    12901 ggttcagcaa gtgaaacctt tgctatcttg gacaatgcgg atattgtgtt cccttatgtc
    12961 ctagacgatg atggtaaaga agttcagcta tctcatggga cttacacacg tttgatggag
    13021 tctaaaaaac gtgaggttcg tcgtggtgcc tatcaagctc tttatgcgac ttacgaacaa
    13081 ttccaacaca cctatgccaa aaccttgcaa accaatgtta aggtgcaaaa ttaccgtgct
    13141 aaagttcgta actacaagag tgctcgtcat gcagccctcg cagcgaattt tgttccagaa
    13201 agtgtttatg acaatttggt agcagcagtt cgcaagcatt tgccactctt acatcgctat
    13261 cttgagcttc gttcaaaaat cttggggatt tcagatctca agatgtacga tgtctacaca
    13321 ccgctttcat ctgttgaata cagttttacc taccaagaag ccttgaaaaa agcagaagat
    13381 gctttggcag tcttgggtga ggattacttg agccgtgtta aacgtgcctt cagcgagcgt
    13441 tggattgatg tttacgaaaa tcaaggcaag cgttcaggtg cctactctgg tggttcttac
    13501 gataccaatg cctttatgct tctcaactgg caggacaatc tggacaatct ctttactctt
    13561 gttcatgaaa caggtcacag tatgcattca agctatactc gtgaaactca gccttatgtt
    13621 tacggagatt actctatctt tttggctgag attgcctcaa ctaccaatga aaatatcttg
    13681 acggagaaat tattggaaga agtggaagac gacgcaacac gctttgctat tctcaataac
    13741 ttcctagatg gtttccgtgg aacagttttc cgccaaactc aatttgctga gtttgaacac
    13801 gccattcacc aagcagatca aaatggggag gtcttgacaa gcgatttcct aaataaactc
    13861 tacgcagact tgaaccaaga gtattatggt ttgagtaagg aagacaatcc tgaaatccaa
    13921 tacgagtggg ctcgtattcc acacttctac tataactact atgtatatca atattcaact
    13981 ggctttgcgg ccgcctcagc cttggctgaa aaaattgtcc atggtagtca agaagaccgt
    14041 gaccgctata tcgactacct caaggcaggt aagtcggact atccacttaa tgtcatgaga
    14101 aaagctggtg ttgatatgga gaaggaagac tacctcaacg atgcctttgc agtctttgaa
    14161 cgccgtttaa atgagtttga agcccttgtt gaaaaattag gattggcata aaatggttga
    14221 atcgtatagt aagaatgcta accataacat gcgtcgtcct gtcgtcaaag aagaaattgt
    14281 agacttgatg cgtcagcgtc aaaagcaggt cacaggtttc ttgaaagaat tggaagactt
    14341 tgcccgcaag gaaaatattc ctattattcc ccatgaaacg gttgcttatt tccgttttct
    14401 tatggaaacc atgcagccta aaaatattct ggaaattggg acggctatcg gtttttcagc
    14461 tctcttgatg gctgaacatg cgccaaatgc taagattaca actattgatc gtaatccaga
    14521 aatgattggt tttgccaagg aaaattttgc ccagtttgac agtcgcaagc aaatcactct
    14581 cctagaggga gatgcggtgg atgtcttatc tacactgaca gagtcttatg atttcgtctt
    14641 tatggattct gccaagtcta aatacatcgt ctttctgcca gaaatcctca aacatttgga
    14701 agttggtggt gtggttgtct tggatgatat ttttcaaggt ggtgatgttg ccaaggatat
    14761 tatggaagtc cgtcgtggtc agcgaaccat ttatcgaggc cttcaaaaat tatttgatgc
    14821 aaccttagac aatccagaac tcaccgcaac attagtgcct ttaggagatg gtattctcat
    14881 gcttcgtaaa aatgtagcag atgttcaact gtctgaaagc gaatgatttt cagaaaaatt
    14941 taagaaaaaa tagtaaaata gatagagtaa cacttatctc aaaggagtag acatgaagaa
    15001 aaaattattg gcaggtgcca tcacactatt atcagtagca actttagcag cttgttcgaa
    15061 agggtcagaa ggggcagacc ttatcagcat gaaaggggat atcatcacag aacatcaatt
    15121 ttatgagcaa gtgaaaaaca acccttcagc ccaacaagtc ttgttaaata tgaccatcca
    15181 aaaagttttt gaaaaacaat atggctcaga gcttgatgat aaagaggttg atgatactat
    15241 tgccgaagaa aaaaaacaat atggcgaaaa ctaccaacgt gtcttgtcac aagcaggtat
    15301 gactcttgaa acacgtaaag ctcaaattcg tacaagtaaa ttagttgagt tggcagttaa
    15361 gaaggtagca gaagctgaat tgacagatga agcctataag aaagcctttg atgagtacac
    15421 tccagatgta acggctcaaa tcatccgtct taataatgaa gataaggcca aagaagttct
    15481 cgaaaaagcc aaggcagaag gtgctgattt tgctcaatta gccaaagata attcaactga
    15541 tgaaaaaaca aaagaaaatg gtggagaaat tacctttgat tctgcttcaa cagaagtacc
    15601 tgagcaagtc aaaaaagccg ctttcgcttt agatgtggat ggtgtttctg atgtgattac
    15661 agcaactggc acacaagcct acagtagcca atattacatt gtaaaactca ctaagaaaac
    15721 agaaaaatca tctaatattg atgactacaa agaaaaatta aaaactgtta tcttgactca
    15781 aaaacaaaat gattcaacat ttgttcaaag cattatcgga aaagaattgc aagcagccaa
    15841 tatcaaggtt aaggaccaag ccttccaaaa tatctttacc caatatatcg gtggtggaga
    15901 ttcaagctca agcagtagta catcaaacga atagtccaaa tcaatgagtc agggaaaaaa
    15961 actcgacttc aggaaaaaat gaagcaaaca ttcccacaat aaaacgcata gtacaaggtt
    16021 tgtactgacc ccaaaagtta gacaattaat ttatctgaag gatttagttc tgtattgcac
    16081 agggctaagt ccttttagtt ttaccttaat tctcttattg ttgtaataat caatatagtc
    16141 tataatggct tgttccaatt gattaagtga tttaaatgtt tgctcatagc cataaaacat
    16201 ttcggatttt aaaatgccaa agaaagattc catcctatcg ttgtcttggc tgtttccttt
    16261 gcgtgacata gatgcttgaa ttcccttact ctctaggaag caatgataag aatcgtgttg
    16321 gtattgccag ccttggtcac tatggagaat cgtattctcg tagtgcttct ctttgaatgc
    16381 ctgttccaac attaacgatc aatcaattta atcatgtacc taatattaga attgtttatc
    16441 ccaaatttat ttgaaagct
//
LOCUS       DAVBNO010000043        15722 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVBNO010000043 DAVBNO010000000
VERSION     DAVBNO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15722
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.043"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(1..218)
                     /locus_tag="VQ846_001532"
                     /pseudo
     CDS             complement(1..218)
                     /locus_tag="VQ846_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            343..801
                     /locus_tag="VQ846_001533"
     CDS             343..801
                     /locus_tag="VQ846_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET3076090.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            814..1422
                     /locus_tag="VQ846_001534"
     CDS             814..1422
                     /locus_tag="VQ846_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076091.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            1493..1825
                     /locus_tag="VQ846_001535"
     CDS             1493..1825
                     /locus_tag="VQ846_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076092.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            1836..3251
                     /gene="ply"
                     /locus_tag="VQ846_001536"
     CDS             1836..3251
                     /gene="ply"
                     /locus_tag="VQ846_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET3076093.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            4132..4848
                     /locus_tag="VQ846_001537"
     CDS             4132..4848
                     /locus_tag="VQ846_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET3076094.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            4972..5441
                     /locus_tag="VQ846_001538"
                     /pseudo
     CDS             4972..5441
                     /locus_tag="VQ846_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5458..6279
                     /locus_tag="VQ846_001539"
                     /pseudo
     CDS             5458..6279
                     /locus_tag="VQ846_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            6286..7257
                     /locus_tag="VQ846_001540"
     CDS             6286..7257
                     /locus_tag="VQ846_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET3076095.1"
                     /translation="MDFLLMGALFIVLPRTMVSAKRINQVLDLHSSIQNPAQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSIGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSATAHAGQRVAIVGPTGAGKTTIVNLLMKFYEIDKGSIRI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVGQKQLLTIARALLKDAPLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSTIRNADLILVMKDGNIIEQGNYEELMV"
     gene            7383..7745
                     /locus_tag="VQ846_001541"
     CDS             7383..7745
                     /locus_tag="VQ846_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HET3076096.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLTCLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            7742..8245
                     /locus_tag="VQ846_001542"
     CDS             7742..8245
                     /locus_tag="VQ846_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET3076097.1"
                     /translation="MKNYQEWYRNIRSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            8359..8805
                     /locus_tag="VQ846_001543"
     CDS             8359..8805
                     /locus_tag="VQ846_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3076098.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKSPRLIF
                     YKGTGEYYLDLSEILFFETEGSKIYAHTQKNAYEVRLKLYELEAILPRYFSRVSKSTI
                     VNIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            8810..9484
                     /locus_tag="VQ846_001544"
     CDS             8810..9484
                     /locus_tag="VQ846_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076099.1"
                     /translation="MKKKAFGIVLLVLAALILVQGNFGIPSLGGHIWPLIGIAFFAYQ
                     SVGALLRRHLTSASFTALVALMIANHFYGILPIPNQTLIWAGVLIVLGVNMLTHSNRT
                     WNGKKWWYDGEKNIFIDKEVAFGTGTFYKQDQELVEDQVEVGFGNAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYAK"
     gene            9645..9836
                     /locus_tag="VQ846_001545"
     CDS             9645..9836
                     /locus_tag="VQ846_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET3076100.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            10121..10420
                     /locus_tag="VQ846_001546"
     CDS             10121..10420
                     /locus_tag="VQ846_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET3076101.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            10417..10734
                     /locus_tag="VQ846_001547"
     CDS             10417..10734
                     /locus_tag="VQ846_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET3076102.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            10750..11376
                     /locus_tag="VQ846_001548"
     CDS             10750..11376
                     /locus_tag="VQ846_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET3076103.1"
                     /translation="MIFTYNKEHVSDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            11409..12170
                     /locus_tag="VQ846_001549"
     CDS             11409..12170
                     /locus_tag="VQ846_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET3076104.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            12248..12643
                     /locus_tag="VQ846_001550"
     CDS             12248..12643
                     /locus_tag="VQ846_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET3076105.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            12913..13197
                     /gene="groES"
                     /locus_tag="VQ846_001551"
     CDS             12913..13197
                     /gene="groES"
                     /locus_tag="VQ846_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET3076106.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            13213..14835
                     /gene="groL"
                     /locus_tag="VQ846_001552"
     CDS             13213..14835
                     /gene="groL"
                     /locus_tag="VQ846_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET3076107.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEVTGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            14973..15722
                     /locus_tag="VQ846_001553"
                     /pseudo
     CDS             14973..15722
                     /locus_tag="VQ846_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567576.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT         4686 a         2866 c         3617 g         4553 t
ORIGIN      
        1 gtggcttgag tttgctggat ttacgtggag tttgtgcttg aggatatatc ttcatgagcc
       61 cttgataatt actgtcagcc aagattttac cagcttgtcc gacttttttg ataagctgtt
      121 tttaatacag ctaacatctc tttaaaagtg gtacgctgaa cactaacaag acgcttaaat
      181 cgtgcatcag ttagttgttt acttgcttca taattcatag aactactata ccatattttg
      241 tttcgcagga agtctaataa gttatagtcc agtttttact atactttgat attcaaatat
      301 gttatactat ggttatcaaa gtaaatgaaa ggagttacga atatgacgcc agaagaaatg
      361 tacctgacag agcgattaga cgtacagata gctcattttt taaagaaaag cgttcaacat
      421 cgtaggcgct ataaggtatt aaaaataaca gaaatcgtgg caggtttcct catagctgtc
      481 ttttgtgcta ttcctatgcc aggtgatcgc taccgtttga tttcggttgc cttatccagt
      541 ctcggcttgc tgtgtgaggg gattatcaat ttgtataatg caaaggaaaa ttggatttct
      601 taccaaaaaa ctgcgcaact cctggagaaa gaaaaattcc tctatcaatg ccaaacggag
      661 aaatatgcag gaaagaccaa ggcttttgcc ctatttgtca agacatgcga aggtcttatc
      721 tcagaggaga ttaaccagtg ggaaagtatc cagtcaaaag aagtggcagc tagtgcagat
      781 gctccagtta aaaaagagta ggaggtagag gaaatgtctc aatccagtta cctgtcgccc
      841 ttgctctggt taaaaaaaga agccgataag gaaaagatga gcgcgaccca gtgccagata
      901 tttttctttt actatcaaat gtttgagctc ttatttgcta gagaaagcga catgaaagac
      961 ttatgtctgg gaacgaaagg tttttatttc tcgcagttag agaaaaattt gctttctgga
     1021 gtttcccgat ttctaaaaaa cttggagggg aaagtaactc tcaaggctaa ccaagaagta
     1081 tcagctcgca aagccctttt tctagccttg acaactagcc aatcagattg gcaggagtta
     1141 gctcctgttt ttgattttta tcagactatc gggaggcttg aaaatccttc tctcttgagt
     1201 tctcaggaca gacaacatct gatgtggatt taccagtcag ctttggagaa ggattatatt
     1261 gtcaaggtta ttggcgacaa gcattttgta ttgaagagac aagatgctac taaattgaca
     1321 gcgcgccaaa ctcaaacttt ggaaattctg agtcaatcag aagacttggt caatcctgtc
     1381 tatgttacat taggagaaaa gggggtgctc ttgcttgatt aagagaggag atgttgtagc
     1441 tctttatttg ccttttccga ctattagtag cgatttggct gtgaagaatc atatgtatat
     1501 ctgtattgac aacagcatga ctaaaaacaa agagttggtt aaaaatcaga ccttcaaacc
     1561 agctctcttg accagacgtt tggtcaagaa ctttatgata gaagagccgg atctagctcg
     1621 taatcctttt acaagaccaa ccttgattga cttagataag gtatttatgt tggataatac
     1681 ggttattccg acttcttatc tagccagacg gcgacgcaat gtctcagaag aattgtacga
     1741 ggaaattttg gatcacttag tccaaccacg gctgatttcg ctgaacaagt ctgagtttat
     1801 gcaactcaat ccaggaactt attaggaggt agaagatggc aaataaagca gtaaatgact
     1861 ttatactagc tatgaattac gataaaaaga aactcttgac ccatcaggga gaaagtattg
     1921 aaaatcgttt catcaaagag ggtaatcagc tacccgatga gtttgttgtt atcgaaagaa
     1981 agaagcggag cttgtcgaca aatacaagtg atatttctgt aacagctacc aacgacagtc
     2041 gcctctatcc tggagcactt ctcgtagtgg atgagacctt gttagagaat aatcccactc
     2101 ttcttgcggt cgatcgtgct ccgatgactt atagtattga tttgcctggt ttggcaagta
     2161 gcgatagctt tctccaagtg gaagacccca gcaattcaag tgttcgcgga gcggtaaacg
     2221 atttgttggc taagtggcat caagattatg gtcaggtcaa taatgtccca gctagaatgc
     2281 agtatgaaaa aatcacggct cacagcatgg aacaactcaa ggtcaagttt ggttctgact
     2341 ttgaaaagac agggaattct cttgatattg attttaactc tgtccattca ggcgaaaagc
     2401 agattcagat tgttaatttt aagcagattt attatacagt cagcgtagat gctgttaaaa
     2461 atccaggaga tgtgtttcaa gatactgtaa cggtagagga tttaaaacag agaggaattt
     2521 ctgcagagcg tcctttggtc tatatttcga gtgttgctta tgggcgccaa gtctatctca
     2581 agttggaaac cacgagtaag agtgatgaag tagaggctgc ttttgaagct ttgataaaag
     2641 gagtcaaggt agctcctcag acagagtgga agcagatttt ggacaataca gaagtgaagg
     2701 cggttatttt agggggcgac ccaagttcgg gtgcccgagt tgtaacaggc aaggtggata
     2761 tggtagagga cttgattcaa gaaggcagtc gctttacagc agatcatcca ggcttgccga
     2821 tttcctatac aacttctttt ttacgtgaca atgtagttgc gacctttcaa aatagtacag
     2881 actatgttga gactaaggtt acagcttaca gaaacggaga tttactgctg gatcatagtg
     2941 gtgcctatgt tgcccaatat tatattactt gggatgaatt atcctatgat catcaaggta
     3001 aggaagtctt gactcctaag gcttgggaca gaaatgggca ggatttgacg gctcacttta
     3061 ccactagtat tcctttaaaa gggaatgttc gtaatctctc tgtcaaaatt agagagtgta
     3121 ccgggcttgc ctgggaatgg tggcgtacgg tttatgaaaa aaccgatttg ccactagtgc
     3181 gtaagcggac gatttctatt tggggaacaa ctctctatcc tcaggtagaa gataaggtag
     3241 aaaatgacta gtagaggaga atgcttgcga caaaaagagg cgatgatctc tctgcggata
     3301 tctgaaagtt tatctcttgc ctagcgattt ccattggagt atgctcgtga tgttaaaatg
     3361 aatctatcaa acagaatgtg taaaaaagta aaaagggtag ccaattcctt gatatacagg
     3421 ggattgatta tcctttactt gttttagatg attggaaatt caagaatgac acgtatcgga
     3481 aatattttgc tgtttatatc taatacccat ttatggaaaa aatgaaatta aatatgaaca
     3541 aatcaattgc tagcaatggt ttagaagtgc cagtgtacta gtctaatttc tccatcttgg
     3601 gaacacgtaa attatactct tcgaaaatct cttcaaacca ggtcagccct atccgcaacc
     3661 tcaaaacagt gttttgagca acctgcggct agctttctag tttgcttttt gatttttatt
     3721 gagtattaga ggtttgctta gaaactcttc tattgatttc ttagaataca atagcgccta
     3781 taagaactgt tggatttcaa agtatttact tgaactatga tatgtctgga gtagactgtc
     3841 aacaaaaggt gcagtatgat tgtttttgtc gcttatatct acaaacttca aacagtaatt
     3901 tgagctgact ttgtcaaact tggctgaaac tttctatttg cagataatgg tctagtttgt
     3961 tttttgaatg tggatgagtg tctgttgata aagatagaaa ggtaagacca tcttagcagg
     4021 taaagtgcta tcctagcttg atttgctaat aaaatccatt tcctctagtg aaaatcaaaa
     4081 aaacttgtgt tataataaga aagattaaaa tgtgaaaaaa ggagattcct aatgggacgt
     4141 aaatgggcca atatcgtagc caagaaaacg gctaaagatg gagctaactc taaagtatat
     4201 gcaaaatttg gtgtagaaat ctatgtagca gctaaaaaag gtgatccaga tccagaatca
     4261 aactcagctt tgaaattcgt tatcgaccgt gctaaacaag cccaagtgcc aaaacacatt
     4321 atcgataaag cgattgataa agccaaagga aacacagacg aaacctttac agaaggacgt
     4381 tacgaaggtt ttgggccaaa tggctctatg ctaattgtgg atactttgac ttctaacgtt
     4441 aaccgtacag cagccaatgt ccgtgcagcc tttggtaaaa acggcggaaa catgggcgct
     4501 tcaggttctg tttcttacct ttttgacaac aaaggtgtta ttgtatttgg aggtgaagat
     4561 gcggacgcag tctttgagca attgctcgaa gcggatgtgg atgtggatga cgtagaagca
     4621 caagaaggta caatcacagt ttacacagcg ccaactgacc ttcacaaggc tatcgttgct
     4681 ctccgtgagt ctggtattga agaattccaa gtgactgaat tggaaatgat tcctcagtca
     4741 gaagtggaat tgtcaggcga agaccttgaa acctttgaaa aactttacag cgttcttgaa
     4801 gacgacgaag acgtccaaaa gatttatacg aacgtagatg ggttttaatt taacctatag
     4861 aacatgatcc taagtgagaa aatcacttgg gattttttta atcaagaaaa tagttctcat
     4921 agaaatttaa ttattctcca gagaactatt tttatttaga agggggagat tatggataaa
     4981 ccgatgttag tctttaagcg ttttggtcat caaatttacc tgatggtgca aaaggaagcc
     5041 aaacgttgcg gcattgaatt tatgggtggg ccgcaaggtc aggttgtgcg ttttttggat
     5101 aatcgcgaga aaaaccaaga cttggtcttg attaaagata tcgagcagga actcaatatt
     5161 accaagtctg ttgctagtaa tctggttaag cgtatagtgc aaaatggttt ggtggaattg
     5221 gaggcgagtc ctgttgataa gcgggctaag tttgttcgtc tgacggacaa agcacgttct
     5281 cagatgcaac aggttaaggc ttttttgaac gcatagacaa gcagttgatg gaagacattg
     5341 atgaagatga attactgatt tttgagaaag ttctcggtca actacaggca aatatcaagg
     5401 gaataggagg agagaataaa gaaattagcc aaaagaatta gtagaaaaga atgggggatg
     5461 attttactag ccattctctt tacttgcttt tcggtctatc tagagttgga agtgccgacc
     5521 tatatctcga aaattacgga tttgctaggt agtcaaggaa ctaatttaga tgagttgtgg
     5581 cagccggcaa gcatgatgat gggaatgccc tttcttgcct tcttgtccgt agttgcagtt
     5641 ggattttttg catcccgagt ggcggcttct tatactagta ggctgagaag tgatattttt
     5701 aaccgagttt tggattactc gcagacagag attaagaaat tttcaattcc tagcctcttg
     5761 atgcgtacta ccaatgacat tactcaagtt caaatgttga ttactatgga cttgcaagtg
     5821 gtaacgtgtg gtccaattat ggctatctgg gctattggga agattttagg tcattcagaa
     5881 tactggctct gggccgtact tgtggcagtg attgtcaacg tcctgatgac gaccgttttg
     5941 atgacgctag cctttccaaa acagtccttg attcaggggc tgacagataa actgaacagt
     6001 atcactcgtg agagtttaac aggtattcgt gtcgttcgtg cctacaatgc agaggattat
     6061 caaaatgaaa aatttgcagc agtaaatgat gaattgaccc gttaaaattt gtttgtcaac
     6121 cgtcttatgg ctattttgaa tcctatcatg atggggattt caagtggttt gagtgtggcg
     6181 atttactgga ttggggccta tgtgattaac gacgctgctc cgatagcgcg tctgcctttc
     6241 tttagtgaca tgattgtttt catgtcttat gccatgtagg ttgtcatgga cttccttctc
     6301 atgggagcac tcttcatcgt tcttccccga actatggtct ctgctaagcg gattaatcaa
     6361 gttttagatt tgcattcttc tatccaaaac cctgctcaag tgcagctgac tgatgaaaac
     6421 ttcaaaggtc aggtcgagtt taaggatgtg accttccgct atgcggcaaa ttcggaggca
     6481 gttattgaac atgttagctt taaagcagaa actggtcaaa cagtggcctt tattgggtca
     6541 ataggttctg gtaaatcaac tctggtcaat ctgattccac gtttctacga cgtgtcagca
     6601 ggagaaattc tggtggacgg tgtcaatgtt caagactatg acttctctgc gacagctcat
     6661 gctggtcaaa gggttgccat tgttgggccg actggggctg gtaagacaac cattgtcaat
     6721 cttttgatga aattctatga gattgataag ggaagtattc gcattgatgg tgtggatacc
     6781 aaggctatga cgcgttcaga agtgcatgat gccttttcaa tggtcttgca ggatacctgg
     6841 ctctttgaag gaactattcg agacaatctc atctataatc aaatagggat tagtgatgaa
     6901 cgaatgatgg aagctagtaa ggctgtggga attcaccact ttattatgac cttgccagat
     6961 ggctatgata ccatcttgga tgacaccgtg accttgtctg taggacaaaa acaactattg
     7021 actattgctc gtgcccttct taaggatgca ccgcttttga ttttggatga ggcgacttct
     7081 tctgttgaca cacggacaga ggaattgatc caaaaagcca tggaccgttt gatggaagga
     7141 cgcacatcct ttgtcattgc ccaccgcttg tcaaccatcc gaaatgcaga cttgatcttg
     7201 gtcatgaaag atggaaatat catcgagcaa ggcaactatg aggaactgat ggtgtaaggt
     7261 ggcttctacg ctgacttgta caatagtcaa tttacagaag atgaagcaga agaataaatc
     7321 aaaagaaggc ttgacggcct tctttttctt atttatacta gtacacatag aaagagagaa
     7381 gtatgagtca gaaattatac aatatgaaat ttgctgctgt ctacttagcc ttgattgcaa
     7441 aagttgagcg aaagggtgga aaggcagagt cagttcacca agtgaccagt tggttgacag
     7501 ggtatgaagt gagtgacgtt cttacttgtc tggataggga tgttacttac ggagattttt
     7561 ttcgacaagc accatattat gtccctgaac gaattgcaat tacagggaag atttgcggtg
     7621 tccgtattga ggaaattgat gatcctctga tgcaggaaat acgtcgcttg gacaagctag
     7681 tggattggct tgccaagggg aaaacatctc aacaagttct agaaaagtat gagaagcata
     7741 aatgaaaaat tatcaagaat ggtatcgaaa tatcagatcc agactaacta gtcgtccaat
     7801 tcttttattc ctattacgca gtttcaatcg tttgatgaca gtcgccatgc ccctggtcta
     7861 tctgatcttg ctagtcacca cttacctgca actaggactt ggtcagcaag ttggagttta
     7921 tgtgcttatt cctgcatcag gttttgtggc cttgtccctg tttcgtaaaa aaatcaatca
     7981 cccgagacct tatgaaactt gggatatctg tccccttctt gataaggata gttcgggacg
     8041 gtcgatgccc agtcgccatg tcttttctgc aactatcatc tccatggcct gtctgcatgc
     8101 tagtctacct attggcttag tatgcttgat cttttcagct ttgctggggt tggtgagggt
     8161 tttagggggt gttcattatc ccaaggatgt cttggttggc tatgcttgtg gtctggtgtg
     8221 gggattcctt ttcttcttat tctgacatgt aagttaagct agtcctacaa ccacttactg
     8281 cttcaaattg accacttgct tttttgccct tgtattccta acttagcttt gatatagtag
     8341 agtcagaaag gagatgtgat gaagttacga attgagattg acggcaattt agaggagact
     8401 gaaattgtca tcaagacccc cactttgaca gatgaaattg cagacttgca acggctcttg
     8461 caagaatcca agtctcctag gttgattttt tacaagggga cgggtgaata ttatctggac
     8521 ttgtcggaaa ttctcttctt tgagacagaa ggtagcaaga tttatgccca tacccagaaa
     8581 aatgcctacg aagttcggct caaactctat gagttagagg ctatccttcc tcgctatttt
     8641 agtcgagtat ccaagtcaac gatcgttaat attcggcaaa tctattcggt ggacaagtcc
     8701 ttttcaggaa cgggcaccat ttccttttat caaacccaca aggaggttca tgtgtcacgg
     8761 cattaccaat ccctcctaaa agaaaatcta agaaatatga ggtaagaaca tgaaaaagaa
     8821 agcatttggt attgtgttgc ttgtactagc agctttaata ttagtacaag gaaattttgg
     8881 aatcccttct ctaggagggc acatttggcc cttgattggt attgcttttt ttgcctacca
     8941 atcagttgga gctttgttgc gtcgccactt aacttcagcc tcttttacag ctctagtagc
     9001 cttaatgatt gctaaccact tttatggcat tttaccgatt ccaaaccaga cactaatctg
     9061 ggcaggagtt ctaatcgttc tcggagtaaa tatgttgact cattctaaca gaacttggaa
     9121 tgggaaaaaa tggtggtatg atggtgaaaa aaacattttt atagataagg aagttgcttt
     9181 tgggacaggg actttctaca agcaagacca agaattggta gaagaccaag tagaagttgg
     9241 ttttggcaat gcaaaaatct actatgacaa tgcagagatg ttaggtgatt ttgcaacttt
     9301 aaatattgaa gtagcttttg gtaatgcaac tgtctatgtt ccgcaacact ggcgtgtcga
     9361 tttgaaagta gaaacatcct ttggtgcagc caaggcggac gctcctgtag ccccaaccag
     9421 caaaaccttg attatccgtg gagatgtggc ttttgggaag ttggaaattg tctacgctaa
     9481 ataaaaaaat cttcacttca accatcaaaa taggcgtact aagagtagga aattgatgcc
     9541 ttgctctgat ttcagttcta tggttgttag actttaaaaa atgaaatgct gcctttaaaa
     9601 gttgtatatt tttcgatatt ttggctttta cgtttgatgt atctatgtac tacagcgtag
     9661 atgatgtagt gtcgaatgct tttaaaaaac gaatgatatt ggacagtttt tttgccttta
     9721 attgctcagg aaccatgaaa gtcagtactt gggtttatga caagggagaa tggtattatg
     9781 taagttcttc tggttccatg attgcgaatg attgggtcaa agacaatggc aagtagtatt
     9841 atctggcttc atcaggtaag atgcttcgca atacctacac acctgatggt tactacgttg
     9901 gcaacttagg tgcctggcaa taagaataat actctttgaa aatctcttca aaccacgtca
     9961 acgtcgcctt atctacaacc tcaaaaccgt gttttgagca gcctgcggct agctttctag
    10021 tttgctctta gtataagaag tccttttcct taaaggaaga ggctttttac ttgcttttct
    10081 gctgcttcct catttgtcct gaagcctttt ttgtgttaaa atgaatggta tgaaagctaa
    10141 aaaaatgtgg atggcaggct tggctctgct aggtatcgga agccttgctc ttgctacgaa
    10201 aaaagttgca gatgaccgta agctcatgaa gactcaggaa gagttgacag agattgtgcg
    10261 agaccatttt tccgacatgg gggaaattgc gaccctttat gttcaagttt acgaaagcag
    10321 tctggagagc ttggttggtg gcgtcatttt tgaggatggc cgtcattata cctttgtcta
    10381 tgaaaatgaa gacctagtct atgaggagga agtcttatga tacagccagc aagtttagaa
    10441 gaattagcat ctttagtgga aaaagcgggc aagaaggtct tcctttttgt ggcagactgg
    10501 tgtggcgatt gtcgttatat ttatcctgcc ttaccagaga ttgaagagac caatccagag
    10561 ttcaccttta ttcgaatgga ccgagatcag tatatggatt tggccaaact ctgggatgtt
    10621 tacggaattc ctagccttgt tgttctagaa aaggacaagg aaatcggtcg ttttgtcaat
    10681 cgcgaccgta aaagtaagga gcaaattaac gattttttag caggattgaa ataggagaaa
    10741 aaggaaacaa tgatttttac gtataacaaa gaacatgtca gtgatgtcct tatggtcatc
    10801 gtgaaaaata gcggagatgc caaactgaat gtggaacgca aaggcaaggt agcccgtgtt
    10861 ttcctcaaag aaaatgggga aacagtagct tggaacattt ttgaggtttc gagcttgttt
    10921 gaaattgcag agcgcggtca agtcttttta tcagatgagc aagtcgctcg tttgaaccaa
    10981 gaattacagg cggaaggttt tacagaagaa attgttaatg ataaggaacc taagtttgtt
    11041 gttggtgaaa ttgtcgagat ggtagctcat ccagacagtg accacctcaa catctgccaa
    11101 gttgcagtcg caagtgacaa gatagtgcaa atcgttgcag gagcacctaa tgcgcgtgtt
    11161 gggttgaaaa ccattgtggc tcttcctggt gccatgatgc caaaagggaa tttcattttc
    11221 ccaggcgaac ttcgtggtga aaagagtttt gggatgatgt gtagtcctcg tgaattgcat
    11281 ttgccaaatg ctccgcaaaa acgtggggtg cttgaattat cagaagacca agttgtcgga
    11341 acaccgttcg acccagcgaa acactggact gcctaggaac ttgtcagcat ctgatagaag
    11401 gaaataagat ggcaaaaaat gtagtgatta cgggagcaac ctcaggaatc ggtgaagcga
    11461 ttgcgcgtgc ttatctggag cagggtgagg atgtcgttct aacaggacga cggatagaca
    11521 gattagaaat cctcaagtcg gagtttgcag taagctttcc aaatcaaacc gtctggactt
    11581 ttccactaga tgtgacggat atggtcatgg tgaagactgt ttgctctgat attctagaaa
    11641 cgatagggag gattgatatc ttggtcaaca acgccggact ggctcttgac ttggctccct
    11701 atcaagacta tgaggagttg gatatgttga ccatgttgga taccaatgtt aaaggtctga
    11761 tggcggttac tcgctgtttc ttgccagcaa tgataaaagt caatcaaggt catattatca
    11821 atatggggtc aaccgcagga atctacgcct atgctggtgc cgctgtttac tcagctacca
    11881 aggctgcggt taagaccttt tcggatggac tgcgaattga taccatcgca acggatatca
    11941 aggtgacaac cattcagcct gggattgtcg aaacagattt ctcaactgtt cgttttcatg
    12001 gtgataaaga gcgagctgcg tccgtttacc aaggaataga agccttgcaa gctcaggata
    12061 ttgcagacac agtagtctat gtgaccagtc agcctcgccg tgttcagatt acagatatga
    12121 ccattatggc caatcaacag gcgacaggtt tcatgattca taaaaaataa gaaatttcct
    12181 cgaaaagtta caaatttctg taactttttt tgatttccta cgaatagata agtaggagga
    12241 agaaaatatg tataataaag ttatcatgat tgggcgttta acgtctacac cagaattgca
    12301 caaaaccaac aatgacaagt cggtagcgcg agcaactatc gctgtgaacc gtcgttacaa
    12361 agaccaaaac ggtgaacgtg aagctgattt tgtcaatatg gtcctatggg gcagactagc
    12421 agaaactttg gcaagctacg caaccaaagg tagtctcatt tccgttgatg gagaattgcg
    12481 tacccgtcgc tttgagaaaa atggccaaat gaactacgtg accgaagtac ttgtaacagg
    12541 attccaactc ttggaaagtc gtgctcaacg tgccatgcgt gaaaataatg caggccaaga
    12601 tttggcagat ttagtcttgg aagaggaaga attgccattt taatactctt cgaaaatctc
    12661 ttcaaaccac gttagcttta tccacaacat caaagcaatg ctttgagcag cctgcggcta
    12721 gcttcctagt ttgctctttg attttcattg agtataaaca ttaaaaagtc tgagttggtc
    12781 tcaggctttt tatcttgaga aagtcagact tttttcttga ttatttctga ctaagtgata
    12841 caatagaact atgaattagc actcaggtat aaagagtgct aataatatct atctcattat
    12901 ggaggaaatc agatgttgaa accattaggg gaccgtgtgg tcttaaaaat agaagaaaaa
    12961 gaacaaaccg ttgggggctt tgttctcgca ggttcagccc aagaaaaaac caaaacagcc
    13021 caagttgtgg ctactggaca aggtgttcgt accttgaacg gtgacttggt tgctccaagt
    13081 gttaaaactg gagaccgtgt cttagttgaa gcccacgcag gtcttgatgt caaagatggc
    13141 gatgaaaagt acatcatcgt aggcgaagct aacatcttgg caatcattga agaatagaag
    13201 gagaaagtaa gtatgtcaaa agaaattaaa ttttcatcag atgcccgttc agctatggtc
    13261 cgtggtgtcg atatccttgc agacactgtt aaagtaacct tgggaccaaa aggtcgcaat
    13321 gtcgttcttg aaaaatcatt tggctcacca ttgattacca atgacggtgt gactattgcc
    13381 aaagaaatcg aattggaaga ccattttgaa aatatgggtg ctaagttagt atcagaagta
    13441 gcttctaaaa ccaatgatat cgcaggtgac ggaactacga ctgcaacagt cttgacccaa
    13501 gctatcgtcc gtgaaggaat caaaaacgtc acagcaggtg caaatccaat cggtattcgt
    13561 cgtgggattg aaacagcagt tgccgcagca gttgaagctt tgaaaaacaa cgccatccct
    13621 gttgccaata aagaagctat cgctcaagtt gcagccgtat cttctcgttc tgaaaaagtt
    13681 ggtgagtaca tctctgaagc aatggaaaaa gttggcaaag acggtgtcat caccatcgaa
    13741 gagtcacgtg gtatggaaac agagcttgaa gtcgtagaag gaatgcagtt tgaccgtggt
    13801 tacctttcac agtacatggt gacagatagc gaaaaaatgg tggctgacct tgaaaatccg
    13861 tacattttga ttacagacaa gaaaatttcc aatatccaag aaatcttgcc acttttggaa
    13921 agcattctcc aaagcaatcg tccactcttg attattgcgg atgatgtgga tggtgaggct
    13981 cttccaactc ttgttttgaa caagattcgt ggaaccttca acgtagtagc agtcaaggca
    14041 cctggttttg gtgaccgtcg caaagccatg cttgaagata tcgccatctt aacaggcgga
    14101 acagttatca cagaagacct tggtcttgag ttgaaagatg cgacaattga agctcttggt
    14161 caagcagcga gagtgaccgt ggacaaagat agcacggtta ttgtagaagg tgcaggaaat
    14221 cctgaagcga tttctcaccg tgttgcggtt atcaagtctc aaatcgaaac tacaacttct
    14281 gaatttgacc gtgaaaaatt gcaagaacgc ttggccaaat tgtcaggtgg tgtagcggtt
    14341 attaaggtcg gagccgcaac tgaaactgag ttgaaagaaa tgaaactccg cattgaagat
    14401 gccctcaacg ctactcgtgc agctgttgaa gaaggtattg ttgcaggtgg tggaacagct
    14461 cttgccaatg tgattccagc tgttgctacc ttggaattga caggagatga agtaacagga
    14521 cgtaatattg ttctccgtgc tttggaagaa cctgttcgtc aaattgctca caatgcagga
    14581 tttgaaggat ctatcgttat cgatcgtttg aaaaatgctg agcttggtat aggattcaac
    14641 gcagcaactg gcgagtgggt taacatgatt gatcaaggta tcattgatcc agttaaagtg
    14701 agtcgttcag ccctacaaaa tgcagcatct gtagccagct tgattttgac aacagaagca
    14761 gtcgtagcca ataaaccaga accagtagcc ccagctccag caatggatcc aagcatgatg
    14821 ggcgggatga tgtaagcatt ctatagaaaa caacttataa aaaacacaaa aggagggaat
    14881 gactaaccct tctttttata atattcactt ctaaattgat ttgagctctc ctaacttata
    14941 tgataaaata agactagaaa aaggagaaga acatgatcga tgtagaaaaa attctgtgca
    15001 agatgccccc caatcagaag attaattatg accgtgtcat gcagaaaatg gtagaagtat
    15061 ggtagaaaaa tgagcaacgg ccaaccattc tcgtgcatgt ttgctgtgcc ccttgtagta
    15121 cctatacact agaatatttg accaagtatg cagatgtgac catctatttt gccaattcta
    15181 atatccatcc caaggtagaa taccataagc gggtctatgt caccaagaaa tttgttagtg
    15241 attttaatga gcggacagga aatacggttc agtacctaga agctccctac gaacccaatt
    15301 aataccgaaa actagttagg ggactagagg aggagcccga aggtggcgac cgttgcaagg
    15361 tttgttttga ctaccgactg gataaaacag cgcaagtggc tatggacttg ggctttgact
    15421 actttggttc agccttgacc atcagtcctc ataagaattc tcaaaccatc aacagcatcg
    15481 gaatcgatgt gcaaaaaatt tacacgaccc actatcttcc caacgatttc aagaaaaatc
    15541 aaggctacaa acgttcagta gagatgtgtg aggagtatga tatctatcgt caatgttatt
    15601 gtggctgcgt ctatgcagcc caagcccaga atattgacct ggttcaagtt aagaaggacg
    15661 ctacagcatt cttgttgggt aaggatgttg agaaagacta ttctcatatc aaatttatag
    15721 ta
//
LOCUS       DAVBNO010000044        14767 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVBNO010000044 DAVBNO010000000
VERSION     DAVBNO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14767
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.044"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            83..880
                     /locus_tag="VQ846_001554"
     CDS             83..880
                     /locus_tag="VQ846_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET3076108.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGFLMGLPYAFLNKNEDTYVD"
     gene            1028..2287
                     /locus_tag="VQ846_001555"
                     /pseudo
     CDS             1028..2287
                     /locus_tag="VQ846_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604466.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2383..3657
                     /locus_tag="VQ846_001556"
     CDS             2383..3657
                     /locus_tag="VQ846_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET3076109.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VQ846_001557"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VQ846_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET3076110.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMAEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4786..5088
                     /locus_tag="VQ846_001558"
     CDS             4786..5088
                     /locus_tag="VQ846_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET3076111.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5196..5834
                     /gene="udk"
                     /locus_tag="VQ846_001559"
     CDS             5196..5834
                     /gene="udk"
                     /locus_tag="VQ846_001559"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET3076112.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5962..7302
                     /locus_tag="VQ846_001560"
     CDS             5962..7302
                     /locus_tag="VQ846_001560"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET3076113.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7280..7492
                     /locus_tag="VQ846_001561"
     CDS             7280..7492
                     /locus_tag="VQ846_001561"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET3076114.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7489..8364
                     /locus_tag="VQ846_001562"
     CDS             7489..8364
                     /locus_tag="VQ846_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET3076115.1"
                     /translation="MKKQEKLALVELALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQVPFE
                     TESIVSVVESLRING"
     gene            8357..9172
                     /locus_tag="VQ846_001563"
     CDS             8357..9172
                     /locus_tag="VQ846_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET3076116.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEV
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9165..9596
                     /locus_tag="VQ846_001564"
     CDS             9165..9596
                     /locus_tag="VQ846_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET3076117.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9603..11270
                     /gene="recN"
                     /locus_tag="VQ846_001565"
     CDS             9603..11270
                     /gene="recN"
                     /locus_tag="VQ846_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET3076118.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQYGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11272..12000
                     /locus_tag="VQ846_001566"
     CDS             11272..12000
                     /locus_tag="VQ846_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET3076119.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12079..13902
                     /gene="lepA"
                     /locus_tag="VQ846_001567"
     CDS             12079..13902
                     /gene="lepA"
                     /locus_tag="VQ846_001567"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET3076120.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(14106..14186)
                     /locus_tag="VQ846_001568"
                     /pseudo
     CDS             complement(14106..14186)
                     /locus_tag="VQ846_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            14360..>14767
                     /locus_tag="VQ846_001569"
     CDS             14360..>14767
                     /locus_tag="VQ846_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET3076121.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPGK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGH"
BASE COUNT         4396 a         2673 c         3486 g         4212 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatggtttc ttcagaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ttcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat gatatggagg
     1021 tcaccttatg acttgtgatt ttaaatctga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atacgatttt ggcgcttgcc catgctggtg accatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttg tcgatgttga taatttggag gaagtagaag cagttatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac tccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaata ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgcgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggatggctt cagggaaatt ccctcaattt gttgacgagg gttcaagctg
     1741 ccacaatttg agctatactc gtgatgtggg tgcagtagcc tttattatag ctgttcgagt
     1801 tcaattgctt tgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa accctttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttatcat gcattggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccacgtcaaa ggtgacgagg aagaagcacg caaggccatt gataatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgctaaatc gcttgttgtc catccagcaa caatcactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaatc aaatccactt
     2221 gtctatcggt cttgaaaatg tagaagattt gattgaagac ttacgcttag ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg ttttgactca ctgtttttga ttttccctca
     2341 ggcatgatat aatggttaca gaagtctaga aagaggaatg taatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gaaagccagt atgctgaact tttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaggcagga actggccttg
     2521 gctgagcaaa aggctatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattgc agagtcaaat ccaaaacttt gatacagaaa aagaattggc caagaaagaa
     2641 gttgaacaga caagccatca ggccttattg gctaaggaca aggaagtaca ggccttggaa
     2701 aaccagttgg cgaccttgcg tttggagcat gaaaatcaat tgcaaaagac cctttctgac
     2761 ctagaaaaag aacgcaatca ggtcaaaaat cagctcctac tgcaagaaaa ggaaaatgag
     2821 ttgtctttgg cttctgttaa gcaaaactac gaagcccagc tcaaagcagc cagtgaacaa
     2881 gtcgagtttt ataagaattt taaggctcaa caatctacaa aagcgattgg ggaaagccta
     2941 gaacagtatg cagagagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa tgaagcggac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggctgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagtcacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc aattgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgact
     3541 acatctgaaa accaactccg tctagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat caacttaaaa aaagaagcag gaatgacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggccgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaattactc tgggttattc cacgacgact
     3901 gaggatgcta gtggggaagt gatcgcagaa acccctgttt tgtctccctt ggatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta cccagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgcccgatt cacttttcgt gtaaaatgca gtaaagggac ttacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gacgctcttg ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaactcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataaca attgcttgct aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct atatagaaat
     4681 ttagaggtgt gaaatgaagc aatttaaaat tctttcagat aaatatttag agtccattac
     4741 aggttctggt gggaacttag gcccagaatt tggtgtgata attccatgat gcgaaatgag
     4801 tttcgagaaa gggtggagca acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg
     4861 agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa atgaaaaata ccaaacggtc
     4921 atggccaatt tgagtcaagg gttgtcactt tacctcatga cgcatcatta ccaggcacct
     4981 aagtctgtca ttgattttgg tttatggatt gccaaagctc ctagccagga aagagggaga
     5041 ctggctttct tgcagatgct tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca
     5101 tcagagggaa gactctggtg ttttttgctg atgagactat aaattttgta aaaaatgtga
     5161 tagaatagac gacggataaa ataacggagg atagcatgca aaatagacca atcattattg
     5221 gagtgacagg tggttctggt ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt
     5281 ttcctgatga aaagatttcc atgattgagc atgattcata ctacaaggat cagtctcatt
     5341 tgacctttga agagcgtgtc aaaaccaact atgaccatcc ttttgccttt gatacagact
     5401 tgatgatcga gcagattaag gaattgttgg cagggcgtcc ggtggacatc ccgacctacg
     5461 actatacaga gcatacacgg agtagcaaga cctatcgtca ggagcctcaa gatgtcttta
     5521 tcgttgaggg cattttggtc ttggaggaca agcgtctgcg cgatttgatg gatatcaaga
     5581 tttttgtgga tacggatgac gatgtgcgca ttattcgtcg tatcaagcgt gatatggagg
     5641 agcgtggccg tagccttgat agcgttatta accagtactt aggtgtggtc aaaccaatgt
     5701 accaccagtt tatcgagtca actaagcgtt atgctgatat cgtcattcct gaaggggtta
     5761 gcaataccgt ggctatcgac ctgttgacga ccaagattgc aaagattttg gaagaagctc
     5821 gaaacagcaa ataatcacat gtggaggcct tgcctccttt ttctattttt ccctcataat
     5881 ggtacataaa tggagatttt taggcatatt tttggtataa taatacccat gaaagagcaa
     5941 gtaaatgaat agtaggtgga gatggaaaag tatttatcgg taacaacttt gaccaagtat
     6001 ctgaaaatga aattcgataa agacccatac ttggaacggg tctatttaac tggtcaagtt
     6061 tccaactttc gtaaacgacc tactcaccaa tatttctccc taaaggatga ccatgcagtt
     6121 attcaagcga ccatctggtc tgggatttat cagaaattag ggtttgacct ggaagaagga
     6181 atgaagatca atgtgattgg gcgtgtacag gtttatgaac caagtggtag ctactccatc
     6241 atcattgaaa aagctgagcc tgatggggtt ggggcgcttg cgattcagtt tgaacaactt
     6301 aagaaaaaat tgacagaaga aggcctgttt caagaacgct tcaagcaagc tctgccccaa
     6361 ttttctaaga gaattggggt agtaaccagc cgtagtggag ccgttattcg agatattatc
     6421 acgaccgtca gcaggcgatt cccaggtgtt gacatccttc tttatccgac caaggttcaa
     6481 ggtgaagggg ctgcggagga aattgctaga aatattgcgc gtgctaatca acgggacgat
     6541 ttggatttgc tcattattgg tcgtggaggt ggttctattg aggatctctg ggcctttaac
     6601 gaagaaattg tggtacgagc tatttttgaa tctcgtttgc cagttatctc tagtgtgggg
     6661 catgaaacgg atgtgacctt ggcagatttt gtggcagatc gacgcgctgc aacgccaaca
     6721 gcggcggctg aactggcaac acctgtgacc aagttggatg tattagctca tttgcaaaat
     6781 caggaaaaac ggatggtaac ggcagtccga aatgttctat ctaagaaaca agaggctttg
     6841 aaaaaatgca gtcagtctgt tatctttaga caacctgagc gcttgtatga cggttatttg
     6901 caacgcttgg accaactgca actgcgtttg aaacaaagtt tgcgaactcg gatttctgat
     6961 aacaaacaat tagttcaagc aagaactcat caattagtac aattatcacc tgttaccaaa
     7021 atccaacgct atcaagaccg tttaggacag ttggacaagc tcttaggtag ccaaatggcg
     7081 ttagtttatg acgccaaggt tgctgaggcc aagcgacttt cggaagcttt gctcatgttg
     7141 gatactagcc gaatcgtggc gcgtggttat gctattgtca aaaaagaaga gtccgttgta
     7201 gattcggttg agagtttgaa gaaaaaagac caagtaacgc ttttgatgcg agatggtcaa
     7261 gtagaattag aggttaaaga tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact
     7321 ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct ctggaagatg cgattactgc
     7381 ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct acgctggaca aggctgaaaa
     7441 gaccttggtc aaggtcatgc aagaagacgg aacagaaagt gattttgaat gaaaaagcaa
     7501 gaaaaattag ctcttgtcga gttggctttg gaagattttt atggagacca gcagtttgcc
     7561 tctagtttac gggagtctgt tctctattct attcatgctg gtggcaagcg tattcggcct
     7621 tttctcttgt tagaagttct ggaagccttg caggttgcca tcaaacctgc tcacgcgcag
     7681 gtagctactg ccttggagat gattcataca gggagcttga ttcacgatga ccttcctgct
     7741 atggatgatg acgattatcg aagagggcgg ttaaccaatc acaagaaatt cggtgaagct
     7801 atggccattt tggctggaga tgccttattc ttagacccat atgccttgat tgcgcaggca
     7861 gatttgccaa gtcagatcaa ggtggacttg attgccaact tatcccttgc ttcaggtagt
     7921 ctgggtatgg tggcagggca agttttggat atggagggcg aacaccagca cttgtctctg
     7981 gaagaacttc agactattca tgccaataag actgggaagt tactagccta tcccttccaa
     8041 gcggcagcta ttatagctga attgtcacct gaaatgcagg tgaagctgaa aactgtgggt
     8101 gaattgattg gacttgcttt tcaagtcaga gatgatgtac tggatgtgac agctagtttt
     8161 gaggaaatcg gtaagacacc tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc
     8221 ttgttgggct tggaagagtc cattgccttt tgtaaccaaa ccctggatca agctaatgaa
     8281 aaattggaag aaattgccca gcaggttccc tttgaaacag aatcgattgt aagtgtagta
     8341 gaaagtttga gaatcaatgg ctaaggaaag agtggatgta ctagcttata aacaggggtt
     8401 gtttgaaacg agagagcagg ccaagcgagg tgtgatggct ggcctagtcg tagcagtcct
     8461 taatggagaa cggtttgaca agccaggaga gaaaattcca gatgataccg aattaaaact
     8521 caagggggag aaactcaagt atgtcagccg tggtggtttg aaactggaaa aggccttgca
     8581 ggtctttgat ttgtcggtgg atggcgcgac tacgattgat atcggggcct ctactggagg
     8641 ttttaccgat gtcatgctac agaatagtgc caagttggtc tttgcagtcg atgttggtac
     8701 caatcagttg gcttggaaat tacgccaaga cccacgagtt gtcagcatgg agcagttcaa
     8761 tttccgctat gctgaaaaga ctgatttcga gcaggagccg agctttgcca gtattgatgt
     8821 gagtttcatt tcccttagtc tgattttgcc agccttgcac cgtgtcttgg ctgatcaagg
     8881 tcaggtggta gcacttgtca aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa
     8941 tggaattatt cgagatgcta aggttcatca gaatgtcctt gaatctgtaa cagctatggc
     9001 agtagaggta ggtttttcag tccttggctt ggacttttct cccatccaag gtggacatgg
     9061 aaatattgaa tttttagcgt atttgaaaaa agaaaagtca gcaagcaatc agattcttgc
     9121 tgagattaaa gaagcagtag agagggcgca tagtcaattt aaaaatgaat aaaaaagaga
     9181 gacttgaaaa aattagaaga ttggtgacag attatcaaat cggcacgcaa gaagaaattg
     9241 tagaacattt gaaagaagca ggtatcactg ccactcaggc gacggtatcc cgagatatca
     9301 aagagttagg tattgtcaaa attcctttga gagacaacac ctatgtctac gaattgccaa
     9361 aatcaatcgt aaaaagtctg caactggctg aagacaatat cgaatcggct gaattgatgg
     9421 ataagatgat caatctccaa gttattccag gaaatacggc ttttgtaaaa gctcagttaa
     9481 tcgagacttt tgcagacaag atttttagct gtttgactga tgatagctcg attttagtca
     9541 ttgccagaag tggaagtcta gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct
     9601 atatgttact tgaaatttcg ataaaaaact ttgccattat tgaggctatt tccctcaatt
     9661 ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc agggaagtcg attatcattg
     9721 atgccatgaa tatgatgttg ggagctcgtg cgacgacaga tgttattcgt catggtgcac
     9781 caaaggcaga gattgagggg cttttctcag ttgagaatag tcgtctttta caggaaattt
     9841 ttgatgagca aggtttagaa ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa
     9901 atggtcgtag tatcagccgt gtaaatggtc agatggttaa tctgtctgtt ttgcgagcta
     9961 ttgggcaaca tcttgtagat attcatggtc agcatgacca tgaggagtta atgcgtcccc
    10021 aactgcatat ccagatgttg gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa
    10081 cctatcaaac gagttttgat gcctatcgga aaatgcgcaa gcaggttctg gaagtcaaga
    10141 aaaaccaaca ggaacacaag gcacgtatcg aaatgttgga atttcaaatg gcagagattg
    10201 aggcagcaaa cttgcaggct ggagaagact tggctctcaa tcaagagcga gataaactcc
    10261 tcaaccacaa aaatattgcg gatacactga ccaatgccta cagtatgttg gacaatgaag
    10321 atttttcaag tctggccaat gttcgttcag ctatgaatga catggaaagt gtcgaagagt
    10381 atgatcctga ataccgtgaa atttcaagct ctctgtctga gacctactat gttttagaag
    10441 acattagcaa acgtttggaa gctatcattg aggaccttga ttttgatggc aatcgcctca
    10501 tgcaggttga gaatcgtttg gacctccttc atactattac tcgtaagtat ggtgggactg
    10561 ttgatgatgt tttgctttat tttgccaaga ttacggaaga atacaatctc ttgacaggca
    10621 ataatctttc gtctgaggac atggaagcag agcttaagaa gttggaagtc aatcttgtca
    10681 atttggcagg tcaacttgct tctgctcgtc ataatttggc tcagcaactc gaagctgaga
    10741 ttaaacaaga actgcaagat ctttatatgg aaaaagccca gtttcaggtt cgttttagta
    10801 agggaaaatt cagtcgtgag ggaaatgaaa tggttgagtt ttatatttca accaaccctg
    10861 gagaagactt taaacccttg gttaaggttg cttctggagg ggaattatct cgtctcatgt
    10921 tagccattaa gtctgccttt tcacgtaaag aaggcaagac tagcattgtc tttgatgagg
    10981 tggatacggg agtttcaggt cgtgttgctc aagctattgc tcagaaaatt cataaaattg
    11041 gtcagtatgg tcaggttttg gctatctccc atttgccaca agtaattgcg attgcagatt
    11101 atcaattctt tattgagaag attagtaatg accattcaac ggtttcgact gttcgtctct
    11161 tgacagtcga agagcgagtg gaggaagttg ccaagatgtt ggcaggtgat gatgtgacag
    11221 aagcagccct gacgcaagcc agagaattgt tgagaaacag ggagaaataa gatgacagat
    11281 tattatgtaa ttggagatgt tcacggaaaa gctgggatgc tggaagacct tcttaaaacc
    11341 tgggatggtc agacccagtt gctctttcta ggtgatttga ttgacagagg tgaggatagt
    11401 caccgtgttc ttgaaatggt caaggacttg gtggacaatc aaggggctat ctgtttgtct
    11461 ggaaaccacg agtatatgtt tttgacttgg ctagatgacc cagaagaaag ttatgaccat
    11521 tatcgtcgca atggtggaga tacaaccatt aactctatcc taggtcgtcc cttggatgca
    11581 ccagttgatg gagtagaaga tgccaagcgg gttgccgctg aagcggcaga cttggtcgaa
    11641 ttcattcgtc aaatgccatt tgtagtagag acagacaagt atatctttgt tcacgcaggt
    11701 attgatttga ccttggacga ctggcatgaa acaacagatt ataaaaaagt atggcttaga
    11761 aaaccattcc acggagctga aaatcatact ggaaaaacca ttgtcttcgg tcatacaccg
    11821 gtctatggtt tgttacagca agaacgcggt acatctgagc tttggactac agaagatggc
    11881 aagattggaa tggatggagg agctgtttat ggtggtgtcc ttcacgggat tgtctttaca
    11941 gaccaaggaa tgacagaaca ccactttatc gaaaatgacg gctttgtcgc tgaagattag
    12001 tactcctagc agggtatggt cttgtcaaaa tatcaaaaac aatttataat aaatagatac
    12061 cctgaaagga agagaatcat gaacttagaa gaattgaaaa aacgacagga gaagatccgt
    12121 aacttctcta ttatcgccca tattgaccat ggaaaatcaa ctctagcaga ccgcattttg
    12181 gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc agcttttgga tagcatggag
    12241 ctagagcggg aacgtggaat tactattaag ttgaatgcca tcgagttgaa ttacactgca
    12301 aaagatgggg aaacttatat tttccatttg attgacacac cagggcacgt tgactttacc
    12361 tatgaagttt cacgttcgct agctgcctgt gagggtgcta ttttggtggt cgatgcggct
    12421 caaggaattg aggctcaaac tcttgccaac gtttatctgg ctttggataa tgatttggaa
    12481 atcatgccta tcattaataa aattgacctg ccggctgcag atccggagcg cgtgcgtaca
    12541 gagattgaag atgtgattgg tttggatgcc agtgaagcag ttttggcttc tgccaaggct
    12601 ggtattggga ttgaagaaat cctcgagcaa attgtagaaa aagtaccagc accaacgggt
    12661 gatgtgacgg cgccacttaa ggccttgatt ttcgactctg tttacgatgc ttaccgtggg
    12721 gttatcctcc aagtgcgtgt catggacgga gtggtcaaac ctggtgataa gattcagctc
    12781 atgagcaata gtaagacctt tgatgtggcc gaagtcggta tttttacacc aaaagcggtt
    12841 ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg cggcgtctat caagacggtt
    12901 caggatactc gtgtgggtga taccgttacc ttggcaacca atcctgcggc agaaccatta
    12961 catggttata agcagatgaa tcctatggtc tttgcgggtc tctacccaat cgaatcaaac
    13021 aaatacaatg acctgcgtga agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag
    13081 tttgaaccag aaacatctca ggcacttgga tttggtttcc gttgtggatt tcttggactt
    13141 ctccatatgg atgttatcca ggaacgttta gagcgtgagt tcaatattga cctcatcatg
    13201 acagctccgt ctgttattta caaagttaat ttgaccgacg gtgagtctat ggatgtgtct
    13261 aacccatctg agtttccaga cccaactaag attgcgacca ttgaagagcc ttatgtcaag
    13321 gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa tggagctagc tcagcgtaag
    13381 cgtggggact ttgtgactat ggactatatt gatgataacc gtgtcaatgt tatctatcaa
    13441 attcctcttg ctgaaattgt ctttgacttc tttgataaac ttaagtcttc gacacgtggt
    13501 tatgcaagct ttgactacga attgtcagaa tatcgcccat ctaagctggt gaaaatggat
    13561 atccttctca atggagataa ggtggatgcc ctcagcttta tcgttcacaa ggactttgcc
    13621 tacgaacgtg ggaaactcat cgttgataaa ctcaagaaaa tcatccctcg ccaacaattt
    13681 gaagttccaa tccaagcggc tattggacac aagattgtcg ctcgtactga tatcaaggcc
    13741 cttcgtaaga acgtacttgc taaatgttat ggtggtgacg tttctcgtaa gcgtaaactc
    13801 cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca tcggatcagt tgaagttccg
    13861 caagaagcct tcctcagcgt cttgagtatg gatgaagaat aagttatcag aaatgccttt
    13921 ccttgttcac aggaaattta tataagaatc aatagtaaag caggcttaga tggtctgctt
    13981 ttttagtttt tatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttaccgtat
    14041 gtatggttac tgacttcgtc agtttcatct acaacctcaa aatcatgttt tgagctgact
    14101 tcgtcagttt catctacaac ctcaaaactg tgttttgagt aacctgcggc tagcttccta
    14161 gtttgctctt tgattttcat tgagtattag aacagaaaaa tgcttggagt atttgtttgt
    14221 gtgtttattt ttatataaca aactataaac aaaacaaaaa tataaaaaaa gagacaaaaa
    14281 agaatagaaa gtaattaaca acgatttcat atagtgttat acttatagca taaagttaat
    14341 ggaaagaagg gaaaacctta tggtattaga ttatttcttt gacaaaaacc ttgtgttttg
    14401 cttagaagcg gataatcaag aacaactctt tgatcaggta gcaagcttgt tggaagaacg
    14461 agaaattgtc actccaactt atcgtgaagc cttgatcacg cgtgaaaagt catttccaac
    14521 tggtttagat atggaatttc taggaaagga cttgccaaat gtagcgattc ctcacacaga
    14581 tattgttcat aatctagctg aaaaagtggt ggttgttcga ttagaaaaac cagtaacttt
    14641 tcacaatatg attgcgccag gtaaggaagt agaagtatcc ctactcttct ttatcattaa
    14701 caattcaagt tcaagtcaaa caaatattct ggctcagttg atggactttt tcacaggaaa
    14761 tggacat
//
LOCUS       DAVBNO010000045        14491 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVBNO010000045 DAVBNO010000000
VERSION     DAVBNO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.045"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            89..1027
                     /gene="msrB"
                     /locus_tag="VQ846_001570"
     CDS             89..1027
                     /gene="msrB"
                     /locus_tag="VQ846_001570"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET3076122.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            1085..2809
                     /locus_tag="VQ846_001571"
     CDS             1085..2809
                     /locus_tag="VQ846_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3076123.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            2811..4559
                     /locus_tag="VQ846_001572"
     CDS             2811..4559
                     /locus_tag="VQ846_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3076124.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEVSREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            4816..6075
                     /locus_tag="VQ846_001573"
     CDS             4816..6075
                     /locus_tag="VQ846_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET3076125.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    6151..6286
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            6325..6825
                     /locus_tag="VQ846_001574"
     CDS             6325..6825
                     /locus_tag="VQ846_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET3076126.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            6901..7269
                     /gene="rplL"
                     /locus_tag="VQ846_001575"
     CDS             6901..7269
                     /gene="rplL"
                     /locus_tag="VQ846_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET3076127.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            7456..7629
                     /locus_tag="VQ846_001576"
     CDS             7456..7629
                     /locus_tag="VQ846_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076128.1"
                     /translation="MQKKGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVEILVLEGQS"
     gene            7626..8222
                     /locus_tag="VQ846_001577"
     CDS             7626..8222
                     /locus_tag="VQ846_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET3076129.1"
                     /translation="MKRITASQYQTSERYYKLPKLLFESERYKNMKLEVKVAYSILKD
                     RLELSLNSGWIDEEGAVYLVFSNSKLMKLLGCSKSKLLSIKKTLREYGLIDEVQQSSS
                     EKGRMANKIYLGELEHEIPLVLHSDGASVKNRLGQSQKQTGPFLNSAPSETEESETDS
                     LFIEDEEERDTQPILKRKVEKVTKYDRDYIWGLVQDQF"
     gene            8259..8396
                     /locus_tag="VQ846_001578"
     CDS             8259..8396
                     /locus_tag="VQ846_001578"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076130.1"
                     /translation="MTDFERIYQYALDNVRFVRRAEVLAEFVFNGLYSVWNNRVRKGG
                     G"
     gene            8495..9894
                     /gene="dcm"
                     /locus_tag="VQ846_001579"
                     /pseudo
     CDS             8495..9894
                     /gene="dcm"
                     /locus_tag="VQ846_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947343.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            9878..10327
                     /locus_tag="VQ846_001580"
     CDS             9878..10327
                     /locus_tag="VQ846_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076131.1"
                     /translation="MKALNKESILDCDELETELHDAEIKQLDEQLFLMPNYPCEFEVT
                     FLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDHQNLVFVLYGQGYRAEG
                     KEGILTTQVTVKAYDEDKKPINFANLLDSLIVSEYQMEPNLWKVSHD"
     gene            10320..10700
                     /locus_tag="VQ846_001581"
     CDS             10320..10700
                     /locus_tag="VQ846_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076132.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQKYGIEVTPHSISEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFIEMILKNPELCIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMAQTHYLSS"
     gene            10713..10946
                     /locus_tag="VQ846_001582"
     CDS             10713..10946
                     /locus_tag="VQ846_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET3076133.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQVLDLDYEDLFRR"
     gene            10949..11536
                     /locus_tag="VQ846_001583"
     CDS             10949..11536
                     /locus_tag="VQ846_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET3076134.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKLLCIGELTILQILYCASLIS
                     FLWMYGQRKQVVKVNMKTRMKWLGIGFVSLLIISLCFSLIHAQGTTNQANLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALVHHPGTILAWCLYV
                     SLGIFLGMVRYKSDLWGSMGLHLVWNLSVYVLFFL"
     gene            11666..12256
                     /locus_tag="VQ846_001584"
     CDS             11666..12256
                     /locus_tag="VQ846_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HET3076135.1"
                     /translation="MVDKREKLMNSFNQYGFLTFKQVIDENLHYKTLLKMVTEGKIDA
                     EEKGLYRLPDIYLDEWFVIQYRFPKGIFSLETALWLHGLSLTIPFNMTMSFPYGTNTK
                     NIKEADICPIILRSHYSEGIIEIERLPGQFIKVYEIERVLVECLRPVHQVDLQIIAPA
                     FKKYFQQNQIHLHKLFYYAQLFKVTDKLQSYTEVLS"
     gene            12256..13092
                     /locus_tag="VQ846_001585"
     CDS             12256..13092
                     /locus_tag="VQ846_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HET3076136.1"
                     /translation="MFSNANSFKAKIKNISKDKGIPAQQVQQHYLIEQVLKLISTSSY
                     RDSFIVKGGYLIGQMIGLDKRTTMDLDVTLKGTEMSRENLIDIFEEILCSKTDGFSFS
                     VDKLEPIRQDDEYGGFSLKLNATFDTLKEVVFIDITTGDKITPREITYSMTSIFTNES
                     IKIWTYNLETVLAEKLETIISRGLASTRPRDRYDLFTLYKLRKEEINLEVLKNALENT
                     AKKRKSKDTIYNWEEQVRGIEISDYQKELWIRYQRQFKYAKDISFDNSVQVIREIMQQ
                     IF"
     gene            13375..13593
                     /locus_tag="VQ846_001586"
                     /pseudo
     CDS             13375..13593
                     /locus_tag="VQ846_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13893..14180)
                     /locus_tag="VQ846_001587"
                     /pseudo
     CDS             complement(13893..14180)
                     /locus_tag="VQ846_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            14179..14442
                     /locus_tag="VQ846_001588"
                     /pseudo
     CDS             14179..14442
                     /locus_tag="VQ846_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4567 a         2543 c         3046 g         4335 t
ORIGIN      
        1 aaccatacct acggtaaggt gacgctgacg tggtttgaag agattttcga agagtattag
       61 ttaaaaacgt gataaaggag aaataaaaat ggcagaaatt tatctagcag gtggttgttt
      121 ttggggccta gaggaatatt tttcacgcat ttctggagtg ctagaaacca gtgttggcta
      181 cgctaatggt caagtcgaaa cgaccaatta ccagttgctc aaggaaacag accatgcaga
      241 aacggtccaa gtgatttacg atgagaagga agtgtcactc agagagattt tactttatta
      301 tttccgagtt atcgatcctc tatctatcaa tcaacaaggg aatgaccgtg gtcgccaata
      361 tcgaactggg atttattatc aggatgaagc agatttgcca gctatctaca cagtggtgca
      421 ggagcaggaa cgcatgctgg gtcgaaagat tgcagtagaa gtggagcaat tacgccacta
      481 cattctggct gaagactacc accaagacta tctcaggaag aatccttcag gttactgtca
      541 tatcgatgtg accgatgctg ataagccatt gattgatgca gcaaactatg aaaagcctag
      601 tcaagaggtg ttgaaggcca gtctatctga agagtcttat cgtgtcacac aagaagctgc
      661 tacagaggct ccatttacca atgcctatga ccaaaccttt gaagagggga tttatgtaga
      721 tattacgaca ggtgagccac tcttttttgc caaggataag tttgcttcag gttgtggttg
      781 gccaagtttt agccgtccga tttccaaaga gttgattcat tattacaagg atctgagcca
      841 tggaatggag cgaattgaag ttcgttctcg ttcaggcagt gctcacttgg gtcatgtttt
      901 cacagatgga ccgcgggagt taggcggcct ccgttactgt atcaattctg cttctttacg
      961 ctttgtggcc aaggatgaga tggaaaaagc aggatatggc tatctattgc cttacttaaa
     1021 caaataaaac agagagtggg gcttcccact ttcttcattt ctagaatatg aatagaaggg
     1081 atttatgaaa cacctattat cttacttcaa accctacatc aaggaatcaa ttttagcccc
     1141 cttgttcaag ctgttagaag ctgtttttga gctcttggtt cccatggtga ttgctgggat
     1201 tgttgaccaa tctttacctc agggagatca aggtcatctc tggatgcaga ttggcctgct
     1261 ccttatcttt gcagtaattg gcgttttagt ggccttgata gctcaatttt actcagcaaa
     1321 ggcagcagta ggttttgcta aggaattgac aaacgatctt tatcgtcata ttctttcctt
     1381 gccaaaggac agcagagacc gtctgacaac ttctagtttg gttactcgct tgacttcgga
     1441 tacctaccag attcagactg gtatcaatca attcctgcgt ctctttttac gagcgcccat
     1501 tatcgttttt ggtgccattt ttatggctta tcgaatctca gctgagttga ctttctggtt
     1561 cttagtcatg gttgccattt tgaccattgt cattgtaggg ttatctcgat tggtcaatcc
     1621 tctctacagt agtctcagaa agaaaacgga ccaactggtt caggaaacgc gccagcaatt
     1681 gcaagggatg cgggttattc gtgcttttgg tcaagaaaaa cgagagttac agatttttca
     1741 aacccttaac caagtttatg ctagattaca agaaaagaca ggtttcttgt ctagtttatt
     1801 aacacctctg acctatctga ttgtcaatgg aactcttctc gttattatct ggcaaggcta
     1861 tatttcaatt caaggaggag tactcagtca aggtgctctc attgctctta tcaattacct
     1921 cttacagatt ttggtggaat tggtcaagct agccatgctg atcaattccc tcaaccagtc
     1981 ctatatctca gccaagagaa tcgaggaggt ctttgcggaa tctcccgaaa acatccattc
     2041 agaattagaa caaaagcaag ttaccagtgg tcgggtttta caagtccaag aattgacttt
     2101 tacctatcct gatgcggccc agccttctct gagagacatt tcctttgata tgactcaagg
     2161 acaaatcctt ggtatcattg gggggactgg ttctggtaaa tcaagcttgg tgcaactctt
     2221 acttggactt tatccagtag acaaggggaa cattgacctt tatcaaaatg gacgtagtcc
     2281 tcttaatttg gagcagtggc ggtcttggat tgcctatgta cctcaaaagg tcgaactctt
     2341 taagggaact attcgttcca acttgacttt aggtttaaat caagaagtga ctgatcaaga
     2401 actctggcaa gccttggaga ttgcgcaagc taaggatttt gtcagtgata aggaaggact
     2461 cttggatgct ctagttgaag caggagggcg aaatttctca ggtggacaaa aacaaaggct
     2521 gtctatcgca cgagcagtct tgcgccaggc tccgtttctc atcctagatg atgcgacctc
     2581 agcactggac accattacag agtccaagct cttgaaagct attagagaaa attttccaaa
     2641 catgagctta attttgatct ctcaacgaac ctcgacttta cagatggcgg accagattct
     2701 cctcttggaa aaaggtgagt tgctagctgt tggcaagcac gatgacttga tgaaatccag
     2761 tcaagtctat cgtgaaatca atgcatccca acatggaaag gaggactaga atgaaacgac
     2821 aaactgtaaa ccagacgctc aaacgtttag tcgtagattt agcaaaccat cccttcctcc
     2881 ttttcctagc ctttctagga actattgccc aagttggctt atcaatttac ctacctattc
     2941 tgattgggca ggtcattgac caagtcctag tggctggttc atcaccagtt ttttggcaga
     3001 tttttctcca gatgctcttg gtggtaatag gaaatactct ggtacaatgg gccaatcctc
     3061 tcctctataa tcgtctaatc ttctcttata ccagagattt acgagagcga atcatccata
     3121 aactccatcg gttaccgatt gcttttgcgg atcggcaagg caatggagag atggtcagtc
     3181 gtgtaaccac ggatatcgaa cagttggcag ctggcttgac catgattttt aaccaatttt
     3241 tcattggtgt tttgatgatt ttggttagta ttctagccat gctccaaatt catctcctca
     3301 tgactctctt agtcttgctg ttgacgccac tgtccatggt gatttcacgc tttattgcca
     3361 agagatccta tcatctcttc cagaagcaaa cagagacgag gggaattcag actcagttga
     3421 ttgaagaatc gcttagccag cagactataa tccagtcctt caatgctcaa acagaattta
     3481 tccaaagatt gcgtgaggct catgacaact actcaggcta ttctcagtca gccatctttt
     3541 attcttcaac ggtcaatcct tcgactcgct ttgtaaatgc actcatttat gcccttttag
     3601 ctggagtagg agcttatcgt atcatgatgg gttcaacctt gaccgtcggt cgtttagtga
     3661 cttttttgaa ctatgttcag caatacacca agccctttaa cgatatttct tcagtgctag
     3721 ctgagttgca aagtgctctg gcttgcgtag agcgtatcta tggggtctta gatagccctg
     3781 aagtggctga aacaggtaag gaagtcttga ccagtgacca agttaaggga gctatttcct
     3841 ttaaacatgt ctcttttggc taccatcctg aaaaaatttt gattaaggag ttgtctatcg
     3901 atattccagc tggtagtaag gtagccatcg ttggtccgac aggtgctgga aaatcaactc
     3961 ttatcaatct ccttatgcgt ttttatccca ttagctcggg agatatcttg ctggatgggc
     4021 aatccattta tgattataca cgagtatcat tgagacagca gtttggtatg gtgcttcaag
     4081 aaacctggct cacacaaggg accattcatg ataatattgc ctttggcaat cctgaagtca
     4141 gtcgagagca agtaattgct gctgccaaag cagctaatgc agactttttc atccaacagt
     4201 tgccacaggg atacgatacc aagttggaaa atgctggaga atctctctct gtcggccaag
     4261 ctcagctctt gaccatagcc cgagtctttc tggctattcc aaagattctt atcttagacg
     4321 aggcaacttc ttccattgat acacggacag aagtgctggt acaggatgcc tttgcaaaac
     4381 tcatgaaggg ccgcacaagc ttcatcattg ctcaccgttt gtcaaccatt caggatgcgg
     4441 atttaattct tgtcttagta gatggtgata ttgttgaata tggtaaccat caagaactca
     4501 tggatagaaa gggtaagtat taccaaatgc aaaaagctgc ggcttttagt tctgaataag
     4561 ccattctctt ttgaaagttt atggacgaaa aaagttgcct tcgagtgact tttttgttac
     4621 aatagctaga aaaattgttc actgtaatac tcaatgaaaa ttaaagagca aactaggaag
     4681 ctagccgtag gttgctcaaa acactgtttt gaggttgcag atagaactga cgaagtcagt
     4741 aaccacacct acggcaaagt gaatctgaag tggtttgaag agagtacaac ttgtctttta
     4801 gaaaaggagc ctataatgaa agtctttcag catgtaaata tcgtgacttg tgatcaagat
     4861 ttccatgttt atcttgatgg aatcttagca gtcaaggatt ctcaaatcgt ctatgtcggt
     4921 caagataagc cagcgttttt agagcaagct gagcagatta tagactatca gggagcttgg
     4981 attatgcctg gtttggtcaa ttgtcacacc cattctgcaa tgacaggtct gagagggatc
     5041 cgagatgaca gcaatctcca tgaatggctc aatgactata tctggccagc agaatctgag
     5101 tttactcccg acatgactac caatgcggtc aaagaagccc taacagagat gctccagtca
     5161 ggaacaacaa cctttaacga tatgtataat cccaatggtg tggatatcca gcaaatttat
     5221 caggtggtga aaacttccaa gatgcgttgt tatttttctc cgactctctt ttcttcagag
     5281 acagaaacaa ctgctgagac tataagcaga actcgatcca tcatagatga aatcttaaaa
     5341 tataaaaatc caaatttcaa ggttatggta gcacctcatt ctccgtatag ctgcagtaga
     5401 gacttgctgg aagcgagttt ggaaatggca aaagagctaa atattccgct ccatgtccat
     5461 gtggcggaga ccaaggagga gtcaggaatt atcctcaaac gatacggcaa acgccccctt
     5521 gcttttcttg aagaactggg ttatttagat catccgtccg tatttgctca cggggtcgaa
     5581 ttaaacgaga gagaaattga acgcttggca tcttctcaag tggctatcgc ccacaatcct
     5641 atcagtaacc tcaaactggc atcaggaatt gctccaatta tccagctcca aaaagcggga
     5701 gtagcagtcg gaattgcgac tgactcggtt gcttccaata acaatctaga tatgtttgag
     5761 gaaggaagga ctgcagctct tcttcagaag atgaaaagtg gggatgccag ccagtttcca
     5821 atcgaaacag ctctcaaggt actgacaatc gaaggggcta aggtccttgg aatggaaaat
     5881 cagataggaa gtctggaagt cggcaagcaa gcagattttc tggtcattca accacaaggg
     5941 aaaattcatc tccaacctca ggaaaatatg ctgtctcacc tggtttatgc agttaaatct
     6001 agtgatgtag atgatgttta tatcgccgga gaacaggttg ttaagcaagg tcaagtcctg
     6061 acagtagaac tttaaaagaa aaatcacgaa aaattttaaa aaaagtttgc aaaaatcttg
     6121 cattcttttt ttgactatgc tatacttata tacggtttaa aaaaactgcc taagacagta
     6181 ggggagctcg actcataaat atcctaccga ggacaaaacg tatcatgtaa aaagaagcgt
     6241 attgtacttt cgtgtctagg tttgggcgcg tttttctttt tgaaaaattc cccaagcaaa
     6301 ataattacgg aggtgaacac actaatgagt gaagcaatta ttgctaaaaa agcggaacta
     6361 gttgacgtag tagctgaaaa aatgaaagct gctgcatcta tcgtcgttgt agacgctcgt
     6421 ggtttgacag ttgagcaaga tacagttctt cgtcgtgagc ttcgtggaag cgaagttgag
     6481 tataaagtta ttaaaaactc aatcttgcgt cgtgcagctg aaaaagctgg tcttgaagat
     6541 cttgcatctg tatttgttgg accatctgca gtagcatttt ctaatgaaga tgttatcgca
     6601 ccagcgaaaa tcttgaacga cttttctaaa aacgctgaag cacttgaaat taaaggtggt
     6661 gcaatcgaag gcgctgtcgc atctaaagaa gagattcttg cacttgcaac tcttccaaac
     6721 cgcgaaggac ttctttctat gctcctttct gtacttcaag cgccagtgcg caacgttgct
     6781 cttgcagtca aagcggttgc agaaagcaaa gaagacgcgg cttaatctta agctacacag
     6841 cgtagcctag ctacgaaaaa aactattata aaatttaaaa cttatttgga ggaaataaca
     6901 atggcattga acattgaaaa cattattgct gaaattaaag aagcttcaat ccttgaattg
     6961 aacgaccttg taaaagctat cgaagaagaa tttggtgtaa ctgcagctgc tcctgtagct
     7021 gttgctgcag ctgatgcagc tgatgctggt gctgctaaag attcattcga cgttgaattg
     7081 acatctgcag gcgacaaaaa agttggcgtt atcaaagttg tacgtgaaat tactggtctt
     7141 ggtcttaaag aagctaaaga acttgttgac ggtgcaccag cacttgttaa agaaggcgtt
     7201 gcaactgcag aagctgaaga aatcaaagct aaattggaag aagctggagc ttcagttact
     7261 cttaaataat agagtatcgc aatttaattg cggaatccct atttaccagt ctttgagagc
     7321 gataagcgat tgatagaaac tgataaattg atttggtttc ctgccaaaaa ttctttggca
     7381 ggatttttgt tttaataaac tttcgggggt agacactaga ttgtctaccc ctttttttgt
     7441 gtttcggagg taattatgca aaagaaagga cagtcatcac gtcaattcgt gaagagtcat
     7501 ctcatgacta tcatgggagt tgatattgta aaagcaactc agttagttga tgaaatggaa
     7561 caagtaggtt taatccgatt tgatgaattt ggaaatgttg aaattttagt cttggaggga
     7621 caatcatgaa gcgtattacc gctagtcaat atcagacatc agagcgttat tataagctcc
     7681 ccaaactttt atttgagagt gaacggtata agaatatgaa actagaagta aaggtggctt
     7741 attctatttt aaaagatcgt ttagaattat ctctcaatag tggttggata gatgaagaag
     7801 gggcggtcta tttagtattt tctaactcta aactgatgaa gcttttaggc tgttcaaagt
     7861 caaaattact ctccatcaag aaaactttac gtgagtatgg cctaattgat gaagtccaac
     7921 agtcttccag tgaaaaaggt cgtatggcta ataaaattta cttaggggaa ttagaacatg
     7981 agatacccct agtcttacat tcagacgggg ctagtgttaa aaacagatta gggcagtctc
     8041 aaaaacagac ggggccgttt ttaaattcag cccctagtga gactgaagaa agtgagactg
     8101 attctttatt tattgaggat gaggaggaga gggatactca acctatcttg aaaagaaaag
     8161 tagaaaaggt cacaaaatat gatcgagatt atatttgggg attggtacaa gatcaatttt
     8221 gacgagaggg gttttctgaa acagccagtg aaattgctat gactgatttt gagagaatct
     8281 atcagtatgc tcttgacaat gttcgctttg ttagacgagc ggaagtactt gctgaatttg
     8341 tgtttaacgg cctatattct gtttggaata accgtgttag aaaaggaggt ggttaaatgt
     8401 cgccaatcga gtggatatta gttatgctca ttttaatttc aagtggtgtg acactttgga
     8461 cattgatagt tgatcagaaa ggggtaatca agaaatgaga tttattgatt tattttcagg
     8521 tatcggtggt tttcgacttg gaatggaaag tgtcggacac gagtgtattg gattttgtga
     8581 gattgataaa tttgctagag aatcttataa gtccattttt caaacggaag gagaaataga
     8641 atatcatgac attaaagagg taacagatca tgactttaga caatttagag ggcaagtgga
     8701 catcatctgc gggggatttc cttgccaagc tttttcactt gcaggaagac gattgggatt
     8761 tgaagatact agaggaactt tgttctttga aattgctcgg gcggccaaac aaatccaacc
     8821 acgttttctt tttcttgaaa atgttaaagg cctactcaat cacgataagg gacggacgtt
     8881 caccacaatc cttaccacat ttgatgagtt ggggtttgat gttgagtggc aggtgcttaa
     8941 cagtaaggat tttggcgttc cccaaaacag agagagggtg tttattatcg gacattctag
     9001 aaagaaaggt accagactct tatttccttt cagacgagaa ggtcaagcaa ctaactctga
     9061 gactttgaaa gcactaggaa atttgaatcc atcaaaaagt ggcgtgagcg gtaaagtgta
     9121 ttattcagaa ggtcttgcgc caactttagt tcgtggaaaa ggagaaggat ttaaggttgc
     9181 tattccttgt atgacaccag atagattaga caaaagacaa aatggtagac gctttaagga
     9241 aaaccaagag ccgatgttta ctttaaatac tcaggatcgt catggcattg tcgttgttgg
     9301 agatttaccg actagtttta aggagacagg tcgtgtctat ggaagtgagg gcttatctcc
     9361 aacactgact acgatgcaag gaggagataa aatccccaaa atactaattc cagaaccaat
     9421 ccaatttcta aaagtacggg aagcaacgaa aaaaggatat gctcaagcag agattgggga
     9481 ttcaatcaat ttggaaagac caagttctca gtatcgtcgt ggtagagttg gcaaaggtat
     9541 agctaatacg ttaacaactg tggtagagtt ggcaaaggta tagctaatac gttaacaact
     9601 agtggtcaaa tgggagtagt agtggctagc tatgaaggag aagataaaca agtttaccaa
     9661 gtagctggtg ttttaataga tggacaattt taccgtttga gaatacgacg aatcactcct
     9721 aaagagtgtt ttcgattgca tggctttcct gattgggctt ttgaagctgc tagaaaagtc
     9781 tctagtaata gtcagctcta taaacaagct ggtaatagtg taaccgttcc tgtgattgcc
     9841 gcaatcgcaa agaaattaaa agaaatagag gaaaaagatg aaagcattaa ataaagaatc
     9901 aatactagat tgtgatgaat tagaaacaga attacatgat gcagaaatca aacagctgga
     9961 tgaacaatta tttttgatgc ccaattatcc atgtgagttt gaagtaacat ttttagatga
    10021 ttaccataaa aaacacaact acccactatt ttacgagtcc tatcttcaaa acgttatgga
    10081 attccttgaa agtcaagaca taaagaatgg ggttgatgcc tttgtagatg atcatcaaaa
    10141 tctcgttttt gttttatatg gacaaggcta tcgagcagag ggaaaagagg gaatacttac
    10201 aactcaagta actgtaaaag cttatgatga agacaagaaa ccgattaact tcgcaaattt
    10261 attagattcc ttaatcgtct cagaatatca aatggaaccg aatctttgga aggtctccca
    10321 tgattgatct ctatctaagt aaaaatagcc aaagaaatca acttcttttg gatttctttc
    10381 aaaagtatgg catagaggtc actcctcatt ctatttctga aatgacaaag gacaaattaa
    10441 ttgaaatgat gagctattct tcagattgtt ttgagtttct atctcccaat ttattacgat
    10501 ttaagaaccg tgacaatcta agattaacgg atttcattga aatgatatta aaaaatcctg
    10561 aactatgcat caggcttcct cttgcggttt caaataagag agtttatcca aatctgaatt
    10621 tggaagaggc tagagctcta ttgccaagag atacgaaaca attgatttac atggcacaaa
    10681 cacattattt atctagttaa aggagaagca atatggctga acgattttgg gagaacttgt
    10741 ccattatttt ggctgaaaga aatattagct ggattgagtt aaccagaaaa atgtttgcgg
    10801 gagagtttca ctatccaagt gaattgaatc gtttgtatca aaagatacgc cactacaaga
    10861 tggaacaacg aatgcctcaa agtccatggg tagaaaggat tgtgcaggta ttagatttag
    10921 attatgaaga tttatttcgg aggtaactat gaaaagaata attccagttt atatattcca
    10981 acaagtaaat gtcttattgg tatccctata cttactgaaa ttgctttgta ttggtgagtt
    11041 aaccatacta cagattctct attgtgcgtc gctcatttct tttttatgga tgtatggtca
    11101 acgaaaacaa gtggtcaagg tcaatatgaa aactaggatg aaatggcttg gtattggatt
    11161 cgttagccta ctgattataa gcctatgttt tagtctgatt catgctcaag gaaccacgaa
    11221 tcaagcaaac ttaattggcc ttcaacatca ggttccttgg ttttcatttt tattgttctt
    11281 aatcaatgcg agtatggttg aagaatttct gtatcgagaa attttatgga acttggtcag
    11341 aaaattagat attcgagttg ctttgacaag tgttttattt gccttagtac atcatccagg
    11401 aaccattcta gcttggtgtt tatatgtttc acttgggatc tttttaggga tggtgcgcta
    11461 taaatcggac ttatggggaa gtatgggtct acatttggtg tggaacctat cagtctatgt
    11521 cttatttttt ctttaataat agctggtctg tcttacgtat ggattaattt tgaaataaaa
    11581 aaactaaatg aattgatttt aaagaaataa aatgctaaca tatatagaag atgttagcat
    11641 tttatttcta agaaaggaga atggtatggt tgataaaaga gagaaactga tgaactcttt
    11701 caatcagtat ggttttttaa cttttaaaca agtaatagat gaaaatttac actacaaaac
    11761 cttattaaaa atggttacag aaggaaaaat cgatgctgaa gaaaaagggt tatatcgctt
    11821 acctgatatt tatttagatg agtggtttgt cattcagtat cgatttccaa aaggaatctt
    11881 ttctttggag acagcacttt ggttacatgg tttatctttg actatccctt ttaacatgac
    11941 aatgagtttt ccttatggta cgaataccaa aaacattaag gaagcagata tatgtcctat
    12001 tatcttacgc tctcactata gtgaaggaat tattgaaata gagcgtcttc ctggtcaatt
    12061 tattaaagtt tatgaaattg aacgagtttt ggttgaatgt ctaagaccag ttcatcaggt
    12121 ggatcttcaa attattgcac cagcgtttaa gaaatatttt caacagaatc aaattcattt
    12181 acacaaatta ttttattatg cccaactatt taaagtaact gataagttac aatcttatac
    12241 ggaggtgcta tcttaatgtt ttcaaatgcg aatagcttta aagcaaaaat caaaaatatt
    12301 tcaaaagata agggaattcc agctcaacaa gtacaacaac attatttaat tgagcaagtg
    12361 ttaaagctga tttctactag ttcttataga gattccttca ttgtaaaagg agggtatcta
    12421 ataggtcaaa tgattggact agataagcga accacaatgg atttagatgt cactctgaag
    12481 ggaaccgaaa tgagcagaga aaatttgatt gatatctttg aagagattct ttgttctaaa
    12541 actgatggtt tttcattttc agtagataag ctagaaccta ttcgccaaga tgatgaatat
    12601 ggaggatttt cattaaaatt aaatgcaact tttgatacat taaaggaggt tgtttttatt
    12661 gatattacta ctggtgataa gatcacacca agagaaatta cttattcaat gacttctatc
    12721 tttactaatg aaagcatcaa gatatggaca tataatctag aaactgtact tgctgaaaag
    12781 ttagaaacaa tcattagtag gggattagct tcaacacgcc cacgtgatcg atatgatctt
    12841 ttcaccttgt ataaacttag aaaagaagag attaatcttg aagtattaaa aaatgcactt
    12901 gaaaatacag caaaaaaacg taaaagtaaa gatactattt ataattggga agaacaagtg
    12961 agaggaattg aaatttctga ttatcagaaa gagctatgga ttcgttatca acgccaattt
    13021 aagtatgcta aagatatctc atttgataac tctgttcagg ttattaggga aattatgcaa
    13081 caaatatttt agtacaaaag taactcattc gtaaagcagt gagttgcttt ttttattcca
    13141 acaacctcaa acatgtgata aaaatttcaa ctactcttat ctataataaa gctacctgtt
    13201 ctttgaaaat tgaatccact ccgtcttgat tagcgtgcct gtgtaagtag ggaatgagag
    13261 tatttccctg tgaagggcaa ctggcacaag acgtgtgtga gaattttcta acagacacgg
    13321 agtttatcgt ttccagactc aaacgatgcg acaaactaga taaaggagtt aatcatgaag
    13381 gtagtaaact tgtatgattt gaaacaaatg ggaaataaag gtggttgtac catccaattg
    13441 attcatcact ttccttttgg tatgggctta ggacatctca aaaaagacta cattgaattt
    13501 aaacgtgttg gtatctttga tgggaaagca gtagaagtta ctcttcgaga accttattcg
    13561 agagatctac tgcaggttgt caagtcaata aagtaactga caccacgagt ataattatta
    13621 gaaacgtgga atcttattaa attttataat aaatatatta aatgaaaaat tattagatat
    13681 aaacttatgt aataaaacat tgaggaaata ataaaaatct atgggtaatg ttctgaaagt
    13741 acttttaggt taggagagaa atataatgat ttttgaaaat tgattcaaaa aatagcgaat
    13801 tgtagaaaac gcttaacaaa tggtgtataa tagacttcca gcgaaacaaa atatgctaca
    13861 ataaacctat gaattataaa gtaaccttat actcatctgt cttcacaaag cattctagtc
    13921 catctccgat taacgatgga ctttatcatc tccttctcca gtccttgtat gacatcttcc
    13981 aaagttcgaa aggctttatt cttaaatcca cgtttacgaa tctctttcca cacttattca
    14041 atggggttca tttctggtgt gtatggagga ataaatgcaa agccaatatt agtcggaatc
    14101 tttaaggcac ttgatttatg ccatatagca ttgtccataa cgaataaaag ataatcatct
    14161 ggataagctt gtgaaagctt ggctgacagt ggttatcaag ggctcatgaa gatatatctt
    14221 caagcacaaa ctccacgtaa atccagcaaa ctcaagccgc taacagctga agataaagcc
    14281 tataaccatg cgctatctaa ggagagaagc aaggttgaga atatttttgc caaagtaaaa
    14341 acgtttaaaa tgttttcaac aacctatcga aatcatcgta aacgcttcgg attacgaatg
    14401 aatttgattg ctgatattat caatcatgaa ctaggattct agttttgcag gaagtctaat
    14461 aaatgtataa gaaaaaggag gaaaaaggat g
//
LOCUS       DAVBNO010000046        14180 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVBNO010000046 DAVBNO010000000
VERSION     DAVBNO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.046"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..183
                     /locus_tag="VQ846_001589"
     CDS             <1..183
                     /locus_tag="VQ846_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3076137.1"
                     /translation="LTVEDQAYNHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFG
                     LRMNLIAGIINHELGF"
     gene            complement(498..2189)
                     /locus_tag="VQ846_001590"
     CDS             complement(498..2189)
                     /locus_tag="VQ846_001590"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET3076138.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2325..2771
                     /gene="argR"
                     /locus_tag="VQ846_001591"
     CDS             2325..2771
                     /gene="argR"
                     /locus_tag="VQ846_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET3076139.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDKWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2822..5356
                     /gene="mutS"
                     /locus_tag="VQ846_001592"
     CDS             2822..5356
                     /gene="mutS"
                     /locus_tag="VQ846_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET3076140.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5503..7197
                     /gene="patA"
                     /locus_tag="VQ846_001593"
     CDS             5503..7197
                     /gene="patA"
                     /locus_tag="VQ846_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HET3076141.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDNEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(7527..7790)
                     /locus_tag="VQ846_001594"
                     /pseudo
     CDS             complement(7527..7790)
                     /locus_tag="VQ846_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            7988..9754
                     /gene="patB"
                     /locus_tag="VQ846_001595"
     CDS             7988..9754
                     /gene="patB"
                     /locus_tag="VQ846_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET3076142.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISSPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            9893..10582
                     /locus_tag="VQ846_001596"
     CDS             9893..10582
                     /locus_tag="VQ846_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET3076143.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            10586..10765
                     /locus_tag="VQ846_001597"
     CDS             10586..10765
                     /locus_tag="VQ846_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076144.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            10752..12101
                     /locus_tag="VQ846_001598"
     CDS             10752..12101
                     /locus_tag="VQ846_001598"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET3076145.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWETFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            12225..13685
                     /locus_tag="VQ846_001599"
     CDS             12225..13685
                     /locus_tag="VQ846_001599"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET3076146.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVAIDDHDMQISHVIRGD
                     DHIANTPKQLMVYESLGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            13699..14133
                     /locus_tag="VQ846_001600"
     CDS             13699..14133
                     /locus_tag="VQ846_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET3076147.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         4150 a         2739 c         3190 g         4101 t
ORIGIN      
        1 ctaacagttg aagatcaagc ctataaccat gcgctatcca aggagagaag caaggtcgag
       61 aacatctttg ccaaagtaaa aacgtttaaa atgttttcaa caacctatcg aaatcatcgt
      121 aaacgcttcg gattacgaat gaatttgatt gctggtatta tcaatcatga actaggattc
      181 tagttttgca ggaagtctat tatttggtta ggtgaattag tgaagcgttt aggcaagtgt
      241 ctctggttac gacgtcatgg cctctaaatc gattatattt aggggtcatg actagtgaag
      301 cagttagcta gttcgcatat aagcggctag cgtctaacaa ttaggaactt tagttccaat
      361 aactttaaga ttacgacgtt ttaggacata aatcgatcat atttatgtcc taaaactagt
      421 gaagcgccta gccaaagtcc gaataggatt tggcgttagt tacttagatt gctttgcaat
      481 caagtaactt tggcgattta catcttctct ggcgcttcta ctccaagcaa gcgaagggct
      541 tcttttagaa cgactgcggt tgcgtagctg agggctagac ggctgtcgcg ttctgggctt
      601 tcatccaaga tacgtgtatg tgcatagtat ttgttaaagg attgagctag gctaattgca
      661 aatttagcaa tgatagaagg ttcaaagtta tctgccgcac ggttgataat acgtgggaag
      721 tcttgaatga gtttaatgat ttcccagctt tcagtatcat tcaagctata gttgccagct
      781 gtttctggtt tgaaatcggc tttgcgtaag atagattgga tacgagcgta ggcatattga
      841 acgtaaggtc cagtttcacc ctcgaaggat accatagctt ctaggtcgaa gtcgtatcca
      901 tttgtacggt cggttttgag gtcatagaat ttaatggctc caaccccaac agcatgtgct
      961 acttggtctt tgttttctag ttcaggattt ttagcctcga tttggacctt ggcacggcta
     1021 acagcctctg caacagtagg ctctagcaag atgacattcc ctttacgagt agagagcttc
     1081 ttcccttcct ttgtaaccaa accaaaagga acgtgagtaa tgtcgtcact ccagtcgtag
     1141 cccatctctt gcaagacagc tttgagctgt ttaaagtggg cagattgttc ttgaccaacg
     1201 acatagatag atttagcaaa ttggtattcg tttttacggt aaagggctgc agccaagtca
     1261 cgtgtgatat agagagttgc accatcagac ttcttgatga gggctggatg ttcaattcca
     1321 tatttctcaa gattcacaac ttgggcacct tctgattcaa gaagtagtcc tttttcagaa
     1381 agaatgtcta caactgcatc catcttatca ttgtagaagg cttctccgtt atagctgtca
     1441 aattcaacct tcaattcatt gtaaaggcgg ttaaattcca ctaaactttc atcgcggaac
     1501 cattgccaaa gagcgagagc ttcctcatct ccattttcaa gtttacggaa ccattcgcgc
     1561 gcttcttcat ccaagctagg gtcattttca gcttcagcgt tgatgcggac atagagttta
     1621 aggagttcat cgattggatg agcttttaca gcttcttcgt cgccccattt tttgtaggca
     1681 acaatcaaca tcccaaattg tttaccccag tctcccaaat ggttgacctt gaccgtttga
     1741 taaccgattt tttggaaaat atgtgacaag ctatctccga taacagttga acgcaggtgg
     1801 ccaatagaaa atggtttagc gatattcgga ctagacatgt cgataacaac attttcttgt
     1861 ttgccaatat tttggtcagc atagtgttct ttttcagttg taacagcttg caatacttga
     1921 gcagaaatgg cagatttatc aaggaaaaag ttaacgtaag gtcctgttgc gacaactttt
     1981 tcaaaggctt ggctgttcat tttttcagcc agttcagccg caatcatttg tggtgcttta
     2041 cgttcgactt tggcaagaga aaaagcaggg aaagcgatgt cacccatttc tgagttttta
     2101 ggggtttcca gtaactttaa aatagcctct tggtccaggc tatcaatgat gctagccaat
     2161 tcgctagcaa tcaattcttt tgtattcatt aagagctcct ttttggactt ttctactatt
     2221 ttaacacaat tttaaagaaa gaagaaaaaa tttttgaaat ctcttgtttt tttggtataa
     2281 tatggttata aatatagtta taaatatgca cgcaagagga ttttatgaga aaaagagatc
     2341 gtcatcagtt aataaaaaaa atgattactg aggagaaatt aagtacacaa aaagaaattc
     2401 aagatcggtt ggaggcgcac aatgtttgtg tgacgcagac aaccttgtct cgtgatttgc
     2461 gcgaaatcgg cttgaccaag gtcaagaaaa atgatatggt gtattatgta ctagtaaatg
     2521 agacagaaaa gattgatttg gtggaatttt tgtctcatca tttagaaggt gttgcaagag
     2581 cagagtttac cttggtgctt cataccaaat tgggagaagc ctctgttttg gcaaatattg
     2641 tagatgtaaa caaggataaa tggattttag gaacagttgc tggtgccaat accttattgg
     2701 ttatttgtcg agatcagcac gttgccaaac tcatggaaga tcgtttgcta gatttgatga
     2761 aagataagta aggtcttggg agttgctctc aagacttatt tttgaaaagg agagacagaa
     2821 aatggcgata gaaaagctat cacccggcat gcaacagtat gtggatatta aaaagcaata
     2881 tccagatgct tttttgctct ttcggatggg tgatttttat gaattatttt atgaggatgc
     2941 ggtcaatgct gcgcagattc tggaaatttc cttaacgagt cgcaacaaga atgccgacaa
     3001 tccgatccct atggcgggtg ttccctatca ttctgcccaa cagtatatcg atgtcttgat
     3061 tgagcagggt tataaggtgg ctatcgcaga gcagatggaa gatcctaaac aagcagttgg
     3121 ggttgttaaa cgagaggttg ttcaggtcat tacgccaggg acagtggtcg atagcagtaa
     3181 gccggacagt cagaataatt ttttggtttc catagaccgc gaaggcaatc aatttggcct
     3241 agcttatatg gatttggtga cgggtgactt ttatgtgaca ggtcttttgg atttcacgct
     3301 ggtttgtggg gaaatccgta acctcaaggc tcgagaagtg gtgttgggtt atgacttgtc
     3361 tgaggaagaa gaacaaatcc tcagccgcca gatgaatctg gtactctctt atgaaaaaga
     3421 aagctttgaa gaccttcatt tattggattt gcgattggca acggtggagc aaacggcatc
     3481 tagtaagctg ctccagtatg ttcatcggac tcagatgagg gaattgaacc acctcaaacc
     3541 tgttatccgc tacgaaatta aggatttctt gcagatggat tatgcgacca aggctagtct
     3601 ggatttggtt gagaatgctc gctcaggtaa gaaacaaggc agtcttttct ggcttttgga
     3661 tgaaaccaaa acggctatgg ggatgcgtct cttgcgttct tggattcatc gccccttgat
     3721 tgataaggaa cgaatcgtcc aacgtcaaga agtagtgcag gtctttctcg accatttctt
     3781 tgagcgtagt gacttgacag acagtctcaa gggtgtttat gacattgagc gcttggctag
     3841 tcgtgtttct tttggcaaaa ccaatccaaa ggatctcttg cagttggcga ctaccttgtc
     3901 tagtgtgcca cggattcgtg cgattttaga agggatggag caacctactc tagcctatct
     3961 catcgcacaa ctggatgcaa tccctgagtt ggagagtttg attagcgcag cgattgctcc
     4021 tgaagctcct catgtgatta cagatggggg aattatccgg actggatttg atgagacttt
     4081 agacaagtat cgttgcgttc tcagagaagg gactagctgg attgctgaga ttgaggctaa
     4141 ggagcgagaa aactctggta tcagcacgct caagattgac tacaataaaa aggatggcta
     4201 ctattttcat gtgaccaatt cgcaactggg aaatgtgcca gcccactttt tccgcaaggc
     4261 gacgctgaaa aactcagaac gctttggaac cgaagaatta gcccgtatcg agggagatat
     4321 gcttgaggcg cgtgagaagt cagccaacct cgaatacgaa atatttatgc gcattcgtga
     4381 agaggtcggc aagtacatcc agcgtttaca agctctagcc caaggaattg cgacggttga
     4441 tgtcttacag agtctggcgg ttgtggctga aacccagcat ttgattcgac ctgagtttgg
     4501 tgacgattca caaattgata tccggaaagg gcgccatgct gtcgttgaaa aggttatggg
     4561 ggctcagacc tatattccaa atacgattca gatggcagaa gataccagta ttcaactggt
     4621 tacagggcca aacatgagtg ggaagtctac ctatatgcgt cagttagcca tgacggcggt
     4681 tatggcccag ctgggttcct atgttcctgc tgaaagcgcc catttaccga tttttgatgc
     4741 gatttttacc cgtatcggag cagcagatga cttggtttcg ggtcagtcaa ccttcatggt
     4801 ggagatgatg gaggccaata atgccatttc gcatgcgacc aagaactctc tcattctctt
     4861 tgatgaattg gggcgtggaa ctgcaactta tgacgggatg gctcttgctc agtccatcat
     4921 cgaatatatc catgagcaca tcggagctaa gaccctcttt gcgacccact accatgagtt
     4981 gactagtctg gagtctagtt tacaacactt ggtcaatgtc cacgtggcaa ctttggagca
     5041 ggatgggcag gtcaccttcc ttcacaagat tgaaccggga ccagctgata aatcctacgg
     5101 tatccatgtt gccaagattg ctggcttgcc agcagacctt ttagcaaggg cggataagat
     5161 tttgactcag ctagagaatc aaggaacaga gagtcctcct cccatgagac aaactagtgc
     5221 tgtcactgaa cagatttcac tctttgatag ggcagaagag catcctatcc tagcagaatt
     5281 agctaaactg gatgtgtata atatgacacc tatgcaggtt atgaatgtct tagtagagtt
     5341 aaaacagaaa ctataaaacc aagactcact agttaatcta gctgtatcaa ggagacttct
     5401 ttgacaattc tccacttttt tgctagaata acatcacaca aacagaatga aaaggagctg
     5461 acgcattgtc gctccctttt gtctattttt taaggagaaa gtatgctgat tcagaaaata
     5521 aaaacctaca agtggcaggc cctggcttcg ctcctgatga caggtttgat ggttgctagt
     5581 tcacttctgc aaccgcgtta tctgcaggaa gtcttaggcg ccctccttac tgggaaatat
     5641 gaagctattt atagtatcgg ggcttggttg attggtgtgg ccgtagtcgg tctagttgct
     5701 ggtggactca atgttgtcct cgcagcctat attgcccaag gagtttcatc cgaccttcgg
     5761 gaggatgcct tccgtaaaat tcaaaccttt tcttatgctg atattgaaca atttaatgcg
     5821 ggaaatctag tcgttcgaat gacaaatgat atcaaccaga ttcagaacgt tgtcatgatg
     5881 accttccaga ttcttttcag acttcccctc ttgttcatcg gttcgtttat cctagcggtt
     5941 caaaccttac cttctctgtg gtgggtgatt gttctcatgg tagtcttgat ttttggtttg
     6001 actgctgtca tgatgggaat gatggggcct cgttttgcca agtttcaaac ccttcttgag
     6061 cgcatcaatg ccattgccaa ggaaaattta cgtggcgttc gtgtggtcaa gtcctttgtc
     6121 caagaaaaag agcaatttgc taagtttaca gaggtctcag acgagcttct tggtcaaaac
     6181 ctttacattg gttatgcctt ttcagtagtg gaacccttta tgatgttggt tggttacggg
     6241 gcggtcttcc tctctatttg gctggtcgcg ggaatggttc agtcggatcc gtctgttgtt
     6301 ggttccatcg cttcttttgt taattaccta agccagatta tctttaccat tgttatggtt
     6361 ggatttttgg gaaattctgt cagccgtgcc atgatttcca tgcgtcgtat tcgagaaatt
     6421 cttgacgcag agccagctat gaccttcaag gatatcccag atgaagagtt ggttggaagc
     6481 cttagctttg aaaatgtgac ctttacctat ccaatggaca atgaaccgat gctgaaagat
     6541 gtgagcttta ctattgaacc tggtcaaatg gttggtgtag ttggagcgac tggtgcagga
     6601 aagtcaacct tggctcaatt gattccacgt ctctttgatc cacagaacgg ggccattaaa
     6661 atcggtggca aggatattcg agaagtgagt gaaggaaccc tgcgtaaaac agtttccatc
     6721 gttctccaac gtgccattct ttttagtgga acgattgcag ataacttgag acaggggaag
     6781 ggaaatgcta ctctatttga aatggagcgc gcagccaata ttgcccaggc tagtgaattc
     6841 attcatcgta tggagaaaac ctttgaaagt ccagttgaag aacggggaac caatttctct
     6901 ggtggacaaa aacaaaggat gtcgattgcg cgtgggattg tcagcaatcc acgtattctg
     6961 atttttgacg attcgacctc agccttggat gccaaatcag agcgcttggt gcaagaagct
     7021 ttgaataagg acttgaaggg gacgacaacc attattattg ctcaaaaaat tagctcggtt
     7081 gtccatgcag acaagatctt ggttctaaat caaggacgat tgattggtca aggcacgcat
     7141 gcagacttgg ttgccaacaa tgccgtttac cgtgaaatct atgaaacaca gaaatgaaag
     7201 acaaactata agaaaagtca atagttttat ctaaattatt tcttatttca atttgatgat
     7261 ttggcgatga ttttagagca cggcaaaaag cccttgaaaa agtccatttt ttcaaaggta
     7321 atcctgtgtt gatttcagaa attacatcac tttttgttcg tcaaatggca gctctttttt
     7381 aggatataaa acagggttcg gataagtttt tttgtaaggt ggatgatggc tacattgtaa
     7441 tgttttcctt attctaactt agtcttaaga taggccttaa aagcaggtga aaagcgaggg
     7501 catgctttgg cagcttgtat gagtgcctac cgcagatgag gggaaccccg tttgatcatt
     7561 cttccagcta aatcaatctg atctgactga taaatagaag aatccagtcc agcgaaagct
     7621 tgtaattgag caggattatc aaaggcatga atattttgaa tctcggctaa aatgaccgcc
     7681 cctaaacgat tcccaatccc agtaaccgtc gtgatgatcg agttgaactc agccatcaag
     7741 tcattgacac atgtttccgc cttgtcaatg agcctcttgt aatgtttgat gttttcatta
     7801 cacgagataa aacgtttatg cgttatcaaa ctcatgacca attgaaacaa aaagctgtgg
     7861 ttagatcctt tcggaaattg tcaagcgatt ggaggaaatg aactaatcca cagcggctta
     7921 ttccaagtat accacttggg ctttggcagt agctaactgc gctaaatata atataaggag
     7981 gagtaaaatg aagacagttc aatttttttg gcattatttt aaggtttaca agttctcatt
     8041 tgtagttgtc atcctgatga ttgttctggc gacttttgcc caagccctct ttccagtctt
     8101 ttctggacaa gcggtgacgc agctagccaa tttagttcaa gcttatcaaa atggcaatcc
     8161 agaacttgta tggcaaagcc tatcaggaat catggtcaat cttggcctgc tggttttggt
     8221 tctatttatc tctagtgtaa tatacatgtg tctcatgacg cgcgtgattg cagaatcgac
     8281 caacgagatg cgcaaaggcc tctttggtaa gcttgctcag ttgacggttt ctttctttga
     8341 ccgtcgacaa gatggcgata tcctgtctca ttttaccagt gatttggata atatcctcca
     8401 agcctttaac gaaagcttga ttcaggtcat gagcaatatt gttttataca ttggtctgat
     8461 tcttgtcatg ttttcgagaa atgtgacgct ggctctcatc accattgcca gcaccccatt
     8521 ggctttcctt atgctgattt tcatcgtgaa aatggcacgc aaatacacca acctccagca
     8581 gaaagaggta gggaagctca acgcctatat ggatgagagc atctcaggcc aaaaagccgt
     8641 gattgtgcaa ggaattcaag aggatatgat ggcaggattt cttgaacaaa atgagcgcgt
     8701 gcgcaaggca acctttaaag gaagaatgtt ctcaggaatt cttttccctg tcatgaatgg
     8761 gatgagcctg attaatacag ccatcgtcat ctttgctggt tcggctgtac ttttgaatga
     8821 taagtctatt gaaacaagta cagccctagg tttgattgtt atgtttgcac aattttcaca
     8881 gcagtactac cagcctatta tccaagttgc agcgagttgg ggaagccttc agttggcctt
     8941 tactggagct gaacgaattc aggaaatgtt tgatgcagag gaggaaatcc gacctgaaaa
     9001 ggctccaacc ttcactaagt tgcaagaaag tgttgaaatc agtcatatcg atttttcata
     9061 cttgcctgat aaacctattt tgaaagatgt cagcatttct tcccctaaag gccagatgac
     9121 agcagttgtt gggccgacag gttcaggaaa aacgactatt atgaacctca tcaatcgctt
     9181 ttatgatgtt gatgctggtg gtatttattt tgatggtaaa gacattcgtg gctatgactt
     9241 agatagtctt agaagcaagg tgggaattgt attgcaagat tcggtcttgt ttagcggaac
     9301 gattcgagac aatatccgat ttggtgtgcc agatgctagt caggaaatgg ttgaggtagc
     9361 agcaaaagca acccacattc acgactatat cgaaagtttg cctgataagt acgatactct
     9421 tattgatgat gaccagagca tcttttcaac agggcagaag caattgattt caatcgctcg
     9481 aaccctgatg acagatccag aagttctcat tctcgatgaa gcaacttcaa acgtagatac
     9541 ggtgacagaa agcaagattc agcatgccat ggaggtggtt gtagcaggta gaactagttt
     9601 cgtcattgcc caccgcttga aaaccattct caatgcagat cagattattg tccttaaaga
     9661 tggagaagtc attgaacgtg gtaaccacca tgaacttttg aagctaggtg gcttttattc
     9721 agaactctat cacaatcaat ttgttttcga atgagaaaga agttgtccta tgtgggcagc
     9781 tttttcttgt ccataaaaaa tgtttatcac agccttaaaa aaaacatatt agacgaaagt
     9841 cattttgagt gatatgatag gactatcgtt agcattcgaa aggagaggca tcatggctag
     9901 aacggttgta ggagttgctg caaatctatg tcccgtagac gcagaaggca aaatcattca
     9961 ttcatctgta tcttgtagat tcgcagagat cattcgtcaa gtcggtggtc tccctttagt
    10021 cattcctgtt ggtgatgagt cagttgtacg tgattatgtg gaaatgattg acaaactcat
    10081 tttgacagga ggccaaaatg ttcatcctca gttttatgga gagaaaaaga ccgtcgagag
    10141 cgatgattac aatctggtcc gtgacgaatt tgaattggca ctcttgaagg aagcgcttcg
    10201 tcagaataaa ccaattatgg caatctgtcg cggtgtccaa cttgtcaatg ttgcctttgg
    10261 tggaaccctc aatcaagaaa tcgaaggtca ctggcaagga ctacctttcg ggacatctca
    10321 ctctattgag acagtagaag gaagcgtggt ggctaagcta tttgggaaag aaagtcaggt
    10381 caattcagtc catcgtcaaa gcattaaaga tttggcaccc aatttccgtg taactgctat
    10441 tgattcaaga gaccaaacta ttgaagcgat tgagtctatc gacgagcacc gcattatcgg
    10501 tttacagtgg catccagagt ttctggttaa tgaagaagat ggcaatttag aattatttga
    10561 gtatttattg aatgaactgt aacgattgga acatctggtc gttctttctt ttattttttc
    10621 aaaattttta gaatgtggta ttttacgcaa acgtttgaat tctgataaaa attggagaaa
    10681 ttcgacaaaa aaacttgaaa aaaacgaagg taagcgttat gatagaaaag aagaaatatt
    10741 ggaggaagaa catgtcacat attaaatttg attattcaaa agttttagac aaatttgttg
    10801 caccacatga agtggaatac atgcaatcac aagtaacagc agcagatgaa ttgatccgta
    10861 aaggaactgg tgctggtagc gacttcttgg gatggttgga ccttcctgaa aaatacgacc
    10921 gcgaagaatt tgaccgcatc ttgaaagctg ctgagcaaat caagtcagac agcgatgttt
    10981 tggttgtgat cggtattggt ggatcttacc ttggtgcaaa agcagcaatc gacttcttga
    11041 atcaccattt tgctaacttg caaacaaaag aagaacgcaa agctccacaa atcctttacg
    11101 caggaaactc aatctcatct acttaccttg ctgacttggt agagtatgtt gcagacaaag
    11161 acttctcagt aaacgtaatt tctaaatcag gtacaacaac tgaaccagcg attgctttcc
    11221 gtgtctttaa agaactcttg gttaagaaat acggtcaaga agaagctaac aaacgtatct
    11281 atgcaacaac tgaccgccaa aagggtgctg ttaaggttga agcagacgct aacggttggg
    11341 aaacatttgt tgttccagat gatatcggtg gacgcttctc agtattgaca gccgttggtt
    11401 tgcttccaat cgcagcatca ggagctgaca tcaaagctct tatggaaggt gcgaatgcag
    11461 ctcgcaaaga ctacacttca gacaaaatct ctgaaaacga agcttaccaa tacgcagctg
    11521 ttcgtaacat cctttatcgt aaaggctatg caactgagat cttggtaaac tatgagccat
    11581 cacttcaata cttctcagaa tggtggaaac aattggctgg tgaatcagaa ggaaaagacc
    11641 aaaaaggtat ctacccaact tcagccaact tctcaactga cttgcactca cttggtcaat
    11701 ttatccaaga aggaactcgt atcatgtttg aaacagttgt ccgtgttgac aaacctcgta
    11761 aaaacgtgct tattcctact ttggaagaag accttgacgg acttggttac cttcaaggaa
    11821 aagacgttga ctttgtaaac aaaaaagcaa ctgacggtgt tcttcttgcc cacacagatg
    11881 gtgatgtacc aaacatgtat gtgactcttc cagagcaaga cgctttcact cttggttaca
    11941 ctatctactt cttcgaattg gcaattgccc tttcaggtta cttgaatgct atcaacccat
    12001 ttgaccaacc aggtgttgaa gcttataaac gtaacatgtt tgcccttctt ggaaaaccag
    12061 gatttgaaga attgagcaaa gaacttaacg cacgtctata atagaagaaa agagtggttt
    12121 acccactctt tttactctct ttatccatag aaattggact cagtcaagac ttgtgatata
    12181 atatagaaag caaaaaggca gatgcctaga taataggaga aactatgtca aaagatatcc
    12241 gcgtacgtta cgcaccaagt ccaacaggac tactacacat cggaaatgct cgtacagcat
    12301 tgtttaatta cttgtatgcg cgccatcatg gtggaacatt tctcatccgt atcgaagata
    12361 ctgaccgtaa acgccatgtc gaggatggtg aacgttcaca acttgaaaac cttcgctggt
    12421 taggcatgga ttgggatgaa agtccagaat cacatgagaa ttatcgccag tctgagcgtt
    12481 tagacttgta tcaaaaatac attgaccaac tattagctga aggaaaagcc tataaatctt
    12541 acgttacaga agaagagttg gcagctgaac gcgaacgcca agaagtagct ggcgaaacac
    12601 cacgctacat caatgaatac cttggtatga gtgaagaaga aaaagcagct tacatcgcag
    12661 aacgtgaagc agcagggatc atcccaactg ttcgtttggc tgtcaatgag tcaggtatct
    12721 acaagtggca tgatatggtc aaaggcgata tcgaatttga aggtggcaat atcggtggtg
    12781 actgggttat ccaaaagaaa gacggttacc caacttacaa ctttgccgtt gccatcgatg
    12841 accacgatat gcaaatctct catgttatcc gtggagatga ccatattgct aatacaccaa
    12901 aacagcttat ggtctatgaa tctcttggtt gggaagctcc agagttcggt cacatgacct
    12961 tgattatcaa ctctgagact ggtaagaagt tatctaaacg tgatactaat acacttcaat
    13021 ttatcgaaga ttaccgaaag aaaggttacc ttccagaagc agtctttaac tttattgctc
    13081 ttcttggttg gaacccaggt ggtgaagatg agattttctc tcgtgaagaa ctcattaaac
    13141 tttttgatga aaaccgcctc agcaagtcac cagcagcctt tgatcagaaa aaactagact
    13201 ggatgagcaa tgattatatc aagaatgcag accttgaaac catctttgaa atggcaaaac
    13261 cattcttaga ggaagcaggc cgtttgactg acaaggctga aaaattagtt gagctctata
    13321 aaccacaaat gaaatcagta gatgagatta tcccattgac agatcttttc ttctcagatt
    13381 tcccagaatt gacagaagca gagcgcgaag tcatgacggg tgaaacagtt ccaacagttc
    13441 ttgaagcatt caaagcaaaa cttgaagcga tgacagatga taaatttgtg acagaaaata
    13501 tcttcccaca aattaaagca gttcaaaaag aaacaggtat taaagggaaa aatcttttca
    13561 tgcctattcg tatcgcagtt tcaggcgaaa tgcatgggcc agaattacca gatacaattt
    13621 tcttgcttgg acgtgaaaaa tcaattcagc atatcgaaaa catgctaaaa gaaatctcta
    13681 aataaagagg atacaataat ggacatctgg gaaaagatgt acgaagaagc acagaaacta
    13741 tacaatccac atgaagtatc agactttgtt tatgccaatc atgttgtagc tgcagtagaa
    13801 gcagaagatg gacaaatatt tacaggattc tgtatggagg gaacctgtgg tgttttccat
    13861 ctctgcgcag aacgggcggc cctcttcaat atgtaccaat tttcaggaca aactaaggtt
    13921 aagaaagtat tagcctttcg agacaaacca ccttatggtg gaagttcagc catgccttgc
    13981 ggtgcttgta gagaattcct tttagagctt aatgctgaaa ataaagatgc agaattcatg
    14041 atggactata atataagaaa aacagttaaa gtcgcagaac taatccctta ttggtgggga
    14101 gaagaacgtg cttctaagtt taatgagcga tagaagagtc agtataattt tggctctttt
    14161 tactataagt tgaaaaaata
//
LOCUS       DAVBNO010000047        13379 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVBNO010000047 DAVBNO010000000
VERSION     DAVBNO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.047"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            451..636
                     /locus_tag="VQ846_001601"
                     /pseudo
     CDS             451..636
                     /locus_tag="VQ846_001601"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1248..1532
                     /locus_tag="VQ846_001602"
     CDS             1248..1532
                     /locus_tag="VQ846_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076148.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            1525..1763
                     /locus_tag="VQ846_001603"
                     /pseudo
     CDS             1525..1763
                     /locus_tag="VQ846_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1859..2077
                     /locus_tag="VQ846_001604"
     CDS             1859..2077
                     /locus_tag="VQ846_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076149.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(2449..4773)
                     /gene="pflB"
                     /locus_tag="VQ846_001605"
     CDS             complement(2449..4773)
                     /gene="pflB"
                     /locus_tag="VQ846_001605"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET3076150.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIDPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            5168..6229
                     /gene="dinB"
                     /locus_tag="VQ846_001606"
     CDS             5168..6229
                     /gene="dinB"
                     /locus_tag="VQ846_001606"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET3076151.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            complement(6376..7221)
                     /locus_tag="VQ846_001607"
     CDS             complement(6376..7221)
                     /locus_tag="VQ846_001607"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET3076152.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            7477..8345
                     /locus_tag="VQ846_001608"
                     /pseudo
     CDS             7477..8345
                     /locus_tag="VQ846_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8646..10535)
                     /locus_tag="VQ846_001609"
     CDS             complement(8646..10535)
                     /locus_tag="VQ846_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET3076153.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFRERTVEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVKEKDQSKQLLTWIFPSILSISLLLSICFYFIYRRKTAPSIK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            10673..12238
                     /locus_tag="VQ846_001610"
     CDS             10673..12238
                     /locus_tag="VQ846_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET3076154.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYN
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKVIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            12238..12978
                     /locus_tag="VQ846_001611"
     CDS             12238..12978
                     /locus_tag="VQ846_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET3076155.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(13087..13302)
                     /locus_tag="VQ846_001612"
     CDS             complement(13087..13302)
                     /locus_tag="VQ846_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076156.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
BASE COUNT         4266 a         2733 c         2488 g         3892 t
ORIGIN      
        1 gaactgacga agtcagtaac atctatacgg taaggcgacg ctgacgtggt ttgaagagat
       61 tttcgaaaag tattagttaa atcttgttta atatctctac accatactat tttacatata
      121 ttatttagcg ccacatagta ctgaactctc tccaaaaacg gttatgcctc tttgaatagg
      181 gcgttatcac aagaaaagca tctccacgtt tcaacttcat atggctcaaa aacaatcaat
      241 tgatgctaaa acctgtacct agatgtttcg gttcataaaa ccatgaaact gtaaaagtgg
      301 atgaaattga tagcgatagt caaatcaaga ggcatcataa ctctaaaaag tcacaatata
      361 taagttcatc ctcggaaaaa tatcattcta attgttgaaa tggatatata aacaggggta
      421 ttaacattaa atgctcatga aacaacgaat acaggtatca aaactatgac aaaacaagtc
      481 cctaaattta ctaaagacac tgctcaactt tacacctgta aatggttgtt gtataataaa
      541 gttacaaaga tgtacgacca cactgttgta aatcatagtg ttcgcgaata tattactgat
      601 agcatttcta caaatacaat tgaagaggct ggataggctt taaacgaaat atgtcagtgt
      661 tttggcattc ctagccttaa tatcatttaa agaattgtat agataaaatt ttttccaata
      721 cagacactcg taacaactgc ttcatttttc taccaacata tttaggaaca ggataagata
      781 caagaggatt aatccataaa tcagttctat accaatctaa gacaaataag ctaaaaaaac
      841 gattgataat aagcaaatag attccaaatt ttctctatct gctcatttta ataaacaata
      901 ctagtgtaac tatccttcca gtcagaaact tgtcaaatca caccgaaaac tcttctaaaa
      961 tttatctcgt taggcaatca agcaaaaact cgacgatagt acaaacatta tcatacagaa
     1021 ttgactgcct aaattatata ctttagtaag gttttcggat aagaaaaaag gttcatttta
     1081 catttctaaa cattcttttc taagatgaaa aacagaattt ttcgattgtg atttaaagca
     1141 acaagaagat tttcagtatc atcctataga tacgagctaa ttaagaaaaa ctacattttt
     1201 gaatataaac tacaataata taaactaaat tttataggag gaagacaatg gattggtacg
     1261 attatatgat acaggcatcc aaacaatcac aattcaacgc aagccattgg tttcgctatt
     1321 tgcgaaaagt tatttttgaa gactattctt atttaacaaa ccaagatgta gaaaagttgc
     1381 tagactccaa agaactaacc cgttttcaaa aaattagctt gaagtatgcc tttcaagagc
     1441 atactccaac tcataaatat gtgatttcat taaataaacc tgctaagtta accaatgttc
     1501 aaaaattgat ggagaaatac aaacatggat aaaatgaaac cggtcttcca agccctaaat
     1561 aagaaattaa ttcaggaaaa tctgacttta acaattatgg ttatgtctta gaatatcatg
     1621 gtttacgtgc cacacaagat gttgatgctt tttatgacca aaatcagaaa atcaatgaaa
     1681 ttattgctcg tgtaggaaaa cagtttaatc ttaacatcca cgaagagtta tggctcaaca
     1741 atcatgttgc aaagcaaata tgaacaaaca acctccctta agtttatgcg aatccttgta
     1801 ctcatttgag aatctgaccg ttttagtagt ccctattgag tatgtgttag gaatgaagat
     1861 gatgagtatc agagagcagg atttgcaaga tattggtgca attattaaat acaaaaactt
     1921 tcattcacca ttcgatacct ttaaatattt aaaagacatg ggatttgata ctattgacct
     1981 ctctgttcta ttagaaggtt ttagttatgc ttatggtatg gattggttag aaaaattttt
     2041 taaagaaaat caagataaac ttcgcgaatt ttattaacag tatcaaacaa gatgattgtc
     2101 aattagccag tcgtcttatt tttatgatga aatgaaatga aataaaatcg gaacaaatca
     2161 cttagagaat tccagtcaat ttctagcact gtttgagccg tttcagcgga ctatagtaaa
     2221 atgaaataag aataggacaa atcgatcagg acagtcaaat cgatttctaa gaatgtttta
     2281 gaagtaaagg tgtactattc tagtttcaat ctattatatt tcagattcgg tccactgttc
     2341 cttcccaaaa acattgcata ttgcgccgaa ggaattgctc atactatccc aagcacaaaa
     2401 ccctcagtcg tttgaccgag ggttcctttt tattattcaa gaacttgatt agctcaatgc
     2461 atccaaggcg tcatccattg aaagaacttc gtggaagaca cgttgtgtca attcagtttt
     2521 ttgttctgga gtgaggtatt tagtgtttac acagtatcca gagatacgta cgataacgtc
     2581 ttcgcctgac atgatttttt cgtaaacatc gttcaagtcc ataacgttca agttaacgtg
     2641 ttgtccaccg ttttcgaagt aaccatcaag gattgttacc aagttatcaa cttgttcatc
     2701 acgagtctta ccaagagcgc gaggtgatac ttgtgtagtc aatgagatac cgtcagctgc
     2761 ataactaaag tcaaggctag aaagtgagtt caagttttgc aaccaaccac ctttagcttt
     2821 gttagatggg ttagcacctg gtgagaagaa ttcaagttta gacaagttca cagaaccatc
     2881 ttcgttgagg tatacacctt tgtgaactgg tgagttacca gtttgtttag agtaagcaac
     2941 gttagatgtg attgtcaaaa gtgatactgt agcttctgcg tctttgtata gtttgtggct
     3001 acgtagacga gttgtgtaag cttcgatcaa ccattctgcc aattcgtttg aacgtgggtc
     3061 atcttcaccc cagcgtgggt agtcaccgat tgtttcgtaa tcgtagatgt agccatcttc
     3121 gtcacggatt ggtttaactg tagcgtattt gatagctgac aatgtatcaa cagtgttagc
     3181 aaatccacag ataccgaatc ccatgttggc acgttgttta gttggcaaga aggccatttg
     3241 aacagcttcg tagttgtacc tatcagtcat gtagtggatg atgttcaagg catctacgta
     3301 agtgtcagtc aaccagtcaa gagatttttc aaagttcgct ttaactgatt cgaattcaag
     3361 aacttcgtca cgaataggat cgatgtcaaa tactttgtag tctttgtgaa catcgtcgta
     3421 accaccattc aaaccagtaa gaagggcttt aagaacgttt acacgagcac cgaagtactg
     3481 gatgttgtgg cgttgttctt cattttctgg atcaagtgga gacacacagc atgagataca
     3541 gctcatttca ccatatccgt ctttagccat tgttgttaca ccttcgtatt ggatagaaga
     3601 gtgtttgtgg ctcatgtgca tacagtagcg acggaagttg tatggcaatt tgtcagtcca
     3661 aagaactgtc aagtttggtt ctggtgagtt accgatgttg tcaagagtgt tcaagaaacg
     3721 gtagtccatc ttagtaacac ggtgacgacc gtcgttaccc ataccagcca tagaagttgt
     3781 gataaaggtt gggtcacctg agtacaattg gtcataagct tttgtacgag caaatttaac
     3841 tgtacgaagt ttcataacga aatcatcaac gaattcttgg atttctgatt cagtaaatgt
     3901 accacgagca aggtcacgtt ctgcaaagat gtccaatacg attggtacac gacctagaga
     3961 tgtagcagca ccgttaatca cacggcagac agccatgaaa gcaatgttaa cccattggat
     4021 tgcttctttc acgttcatcg ctggtttgcg aacatcaacc ccgtaaaggt cacccaggcg
     4081 aacaacttgt tgcaatgctt ggtattgaag gtttacttct tcacgaagac ggattgtttc
     4141 ttcatcgatt tctttgattg cattccagtc gtttactttt tcttgcatca agtagtctgc
     4201 accgtaaaga gcaagacgtg cgtaaacacc gatgatacgt ccgcgtgagt atgcatctgg
     4261 aagaccagtt acagtgtgtg catgacgagc gcgacgaata tttgaagtgt aggcacggaa
     4321 aataccgtcg ttaactgttg ttacatattt agtgaagatt tcgtgaacag ctgggtctgg
     4381 ttcgtatcca ttttctttta aagtagtttc agccatacgg ataccacctt ttggcatgaa
     4441 gttcaatttg aagagttcat cattttggat accaaagata acttcatttt ctttgtcgat
     4501 aaatccagca gggatatcag cgatagatgt tggacgagtg tccattggga aacgagtttc
     4561 ttcgtagtgt gctttagttt cttctacaat tttcttgatg tgaagtgaac gctctgttgg
     4621 tcctgcaagg aagctttcgt ctccatcata aggtgtgtag ttagcttgta caaagcgtga
     4681 tacacttgct ttttctttcc aatctacgcc tttgaagcct tcccaagctt tgtcaaaaat
     4741 atcttgtgct tcaacaaccg tcttaacaac catattaatg tcctcttttt tctagtaaca
     4801 gtcatctgtt acattcataa gacaagtata ccataaagta atcgatttca acaagtgcaa
     4861 aatccctatt tttacacttt cttttctaaa acagtctata ttttatttca aactgtatta
     4921 tatttttgaa aaaaataaag tccttttttc tttttttcag aaaaaagggt ataataaaag
     4981 aaaataagca gtaacactca atggaaatcg aaaagcaaac taggaagcta gccgcaggct
     5041 gctcaaagca ctgctttgag gttgtaaata gaactgacga agtcagctca aaatactgtt
     5101 ttgaggttgc agatggaagc tgacgtggtt tgaagagatt ttcgaagagt ataaaaaggt
     5161 gctaggcatg ttgatttttc ctttgttaaa tgatttgtca agaaaaatca tccatattga
     5221 catggatgcc ttttttgctg cggtggaaat caaggataat cctaaactca gaggaaaacc
     5281 tgtcattatt ggaagcgacc ctcggcaaac aggtggacgg ggagtcgttt ctacctgtag
     5341 ctatgaggct cgagcttttg gtgtccattc tgccatgagt tccaaggaag cttatgaacg
     5401 ttgtccccag gccgtcttta tctcaggaaa ttatgagaaa tacaaagctg tgggactcca
     5461 gattcgagct atttttaagc gctatacaga tttgattgaa cccatgagca ttgacgaagc
     5521 ctatttggat gtgacagaaa ataaactcgg tatcaagtca gcggtcaaaa ttgctcgcct
     5581 cattcaaaaa gatatctggc aagaactcca tctaactgct tctgccggta tttcctacaa
     5641 caagttctta gctaaaatgg cgagtgatta tcaaaaacct catggtttga cagtgattct
     5701 acctgaacag gctgagaatt ttctcaaaca aatggatatt tccaaatttc atggagtagg
     5761 aaaaaagaca gtagaacgtc ttcatcaaat gggcgttttt actggtgctg atttacttga
     5821 agttcctgag gtgaccctaa tagaccgttt tggtagacta ggctatgatc tgtatcgaaa
     5881 ggctcgtggc attcacaact ctccagtcaa atccaatcgc atccgtaaat caatcggcaa
     5941 ggagaaaacc tacgggaaga ttctccgtgc tgaggaagat atcaaaaaag agctgactct
     6001 tctatcagaa aaagtcgctc tcaatctaca tcaacaagaa aaagctggaa aaattgtcat
     6061 tttgaaaatc cgctacgagg acttttcaac tcttaccaaa cgaaaaagtc ttgctcaaaa
     6121 aacacaagat gctagtcaga taagccaaat agccctgcaa ctctatgaag aattaagcga
     6181 gaaagaaaga ggtgtccgcc tattggggat taccctgact ggattttaaa gctcaatgaa
     6241 aatcaaagag caaactagga agctagccgc aggctgctca aaacactgtt ttgaggttgc
     6301 agataaagct gacgcggttt gaagagattt tctaagagta taaaaccttg aagagttgcc
     6361 ccttcaaggt ttttcttata caaataaacg gacaaaactg taaagtagca aaacactacc
     6421 aagcacgata cggtatttac caaaaagggt gaagtcgtgt tttttcacat agctggtcaa
     6481 gaagcgaata gccaccatgc tgaccgcaaa agctactccc atcgcgacca agagcaaaaa
     6541 caattgccca aagctcaaga gttctccggc tttcacaaat ttgaaaatct ttaaggcact
     6601 agctccaaac ataacgggaa tcccaagata gaaggtaaat tctgtcacaa ctgaacgact
     6661 ggttccattt aacaaaccac cgacaatcgt tgcacctgaa cggctagtcc ctggtaaaag
     6721 agcaagaact tggaagagtc cgatatagaa agcggtcgta taaggaagct tgtccaactc
     6781 tgttacactt ggctcgatag cacgcgcttt attgcgcttt tccaaataga tgaaggcaac
     6841 cccgtagata atcaacatga gagcaactga aaccatgtta tggaagtggg tatcaaacca
     6901 atcatcaaat ttaaagacac caagtaaagg caaagtggca atcaagacct tcaaccatag
     6961 tctccaagtc ttacgaactt cctgtttgtc cttagtcggt ttaaaaggat tgagcttgtt
     7021 aaaataaatc accataactg ctaaaatagc accaagctga atcacgacat taaacatgga
     7081 cataaaggct tcattttgat tttggtattg gataaactcc tctgctaaaa tcaagtgacc
     7141 tgtactggaa atcggcaacc attccgtaat tccttcaaca atcccgaaga agatagattt
     7201 taaaatttca ataagataca tatatttctc cttatattat atttaacgca gttagctact
     7261 gccaaagccc aagtggtata cttggaataa gccactgtgg attagttcat ttcctccaat
     7321 cgcttgacga tttccgaaag ggtctaacaa cagctttttg tttcaattgg taatgagttt
     7381 gataacgcat agacgtttta tctcgtctaa ggtaacaagg caaaaagttc gtggataacc
     7441 aagtagactt gaaatactat atctggaggt actcccatgc tttatgtggg cattgatgtc
     7501 gctaaaaata aacacgatgt tacagccttg aatgttcgag gaaaactgtt cttaaaccac
     7561 tcactttttc aaataataaa gctggttttg aactcttaga tctgtctttt cgacagctca
     7621 accaagactg tctcatcgct cttaaacttc tttctgaccc caatcgtgaa caatttcaac
     7681 acgataatcg gcaagtagaa ctaaaaatac tggctagaca tattcatcgt ctcaagaaaa
     7741 aacagtctga ttggaaagta caatacactc gttgtcttga tatcatcttt cctgagttgg
     7801 ataaaatcgt tggaaagcat tcagaatata cctaccaact cttgacacgc taccctaatc
     7861 ctcagaaaag gcttgaggca agatttgata agctgataga aattaagcga ttgaccgctt
     7921 ctaaaattca ggatatcctc tcagtcgcac ctcgttctat cggaacaaca tctcctgctc
     7981 gtgaattcga aatcatcgaa atcatcaaac attacaagag gctcattgac aaggcggaaa
     8041 catgtgtcaa tgacttgatg gctgagttca actcggtcat cacgacggtt actgggattg
     8101 ggggtcgttt aggggcggtc attttagccg agattcgaaa tattcatgcc tttgataatc
     8161 ctgctcaatt acaagctttc gctggactgg attcttctat ttatcagtca ggtcagattg
     8221 atttagctgg aagaatgatc aaacggggtt cccctcatct gcggtgggca ctcatacaag
     8281 ctgccaaagc atgcgctcgc ttttcacctg cttttaaggc ctatcttaag actaagttag
     8341 aataaggaaa acattacaat gtagccatca tccaccttgc aaaaaaactt atccgaaccc
     8401 tgttttatat cctaaaaaag agctgccatt tgacgaacaa aaagtgatgt aatttctgaa
     8461 attaacacag gattaccttt gaaaaaatgg actttttcaa gggctttttg ccgtgctcta
     8521 aaatcatcgc caaatcatca aattgaaata agaaatagtt tagataaaac tattgacttt
     8581 tcttatagtt tgtctttctt ctatcttcca ttatatcata ctttttcagt ctatggtagc
     8641 tctctttaaa aggctccgat actgccgcca cctccgcctc cagagaagcc accgccagaa
     8701 cttccacttc cagaagatac ggagtaggta cttgctgtat ttgcgacact agcataatgg
     8761 ctcatttgcg cgcttgaatg ataaaacata ctgtgccagc cataagctac atagagattg
     8821 atatctggat tttcaacttg aatctgatga accttcatca aatgactaac cttgtccgca
     8881 tagccaaata aggttgcata gaccaagaga cgattccaaa caacaatact ttccaattca
     8941 gccttatcca gtcgtgcaat ctcacgtaac atattttcaa aactggtcca gagatagtag
     9001 acctcagctc ccgcttcatt tagaacacca tcacgattat ctagtcgaag cttccaataa
     9061 tagaaaacag acaaaactaa ccctagaaaa ccaagtattg gcaaagggag gtaaagatag
     9121 ccatgaacgt ctaaactgta caagaacaaa ccaaatccga taaatagggg caagatagtc
     9181 aagacaccca tacccacttg caaagccttt tccaaaccag ttaaaggacg atagtaatct
     9241 gggagccccc agaaggaaac tcgttttctc actccttctt gcatctggtt caatacctct
     9301 tcaaaagaag atttgagttg aagccctctt gcttgaatcc gtttttcatc agaaactttg
     9361 gctctacgat aaagactatc agataccttg taatccgcaa acaaattgga aagagtttct
     9421 tcttttttac ctgaaaaagc tagatttagg cagtctttct caaagcttga caaaccatct
     9481 tcttttacta gcctcaaacc aactgcatct ccttctgaaa tgatagagac attcccacgg
     9541 tctatcacat ctagcaaggt agcttgaata agttgatcaa aggtgaattt tccagctccc
     9601 ttgaccaagg gactcacttc ctccaaggag gtcgagtaga ctgcttctga taaaaccata
     9661 ggctctaatt ccattggggg ttcatagaga cgatgatttt tagcatattt gattgaagga
     9721 gcggtctttc ttctatagat aaaatagaaa cagatgctca ataacaagga gatggaaagg
     9781 atcgaaggga agatccaagt aagtagttgt ttactttgat ctttttcttt aacgatcgaa
     9841 tcctctatct tattaaactc ttctaaacga ttccctttca agccctgatc cctagcgcta
     9901 gcaaaatcag ttcgcggcca gtaggcatgc aattcaactc cacgcttagc cggaagattg
     9961 tctaaacgaa tagtataatc aaggttactc ttttcaaccg ttctctctct aaaaagtttc
    10021 cctgtatgga aaaagagttt ctcagccccc ttgtctcccc ttacatgaaa ttcaaacttt
    10081 tcaatagact ctgaactatc tgtcagaggt tgccaattta attcagcgat atcatcataa
    10141 aggaaaagta aattttttaa gttccagacg aggtcaactt caactatgtc gccctcctga
    10201 cctggattat agactctcac agtataacca tccgcttctt ctgttacttc gctagtcaca
    10261 tctgctagtt ctgcaccgtt tttcgcggcc tgaatctttg gatgagggtc aatgtcaaac
    10321 ccgctaggca tcttaccagc acgtccaagt cccacgattt ggcccttaaa gtcctcctca
    10381 aactggtaaa ctatcttctg tctaaactct gccgtattgt ctgcatgaat atacaaatcc
    10441 ccttgataag agtttatctt gaaatcaatg gcaaaaacag agagtggaag aaggcaaaac
    10501 aagcctaaca ccagtaagaa aaaagttttt ttcatcaact aagccccctt tttatctttc
    10561 ttatcattat agcatttttt cgtaagtaaa ggcttactta tgttgaaaaa ttacgcattt
    10621 ttcgataaaa taggagacag ttatttttta atagatcaga ataggagaaa tcatgagaaa
    10681 aatatactta tctattttca caagtctctt gctgatgcta ggacttgtca atgttgctca
    10741 agccgatgaa tatttacgca tcggtatgga agcagcatat gctcccttta actggaccca
    10801 ggatgatgat agcaacggag ctgtcaaaat cgatgggacc aatcagtatg ccaacggata
    10861 cgatgttcaa atcgccaaga aaatcgctaa ggacttaggt aaagaacctt tggttgttaa
    10921 aaccaagtgg gaaggtctag tccctgccct tacttctggt aagattgaca tgattatcgc
    10981 aggtatgagt ccaactgcag aacgcaaaca agaaattgcc ttttcgagca gttactatac
    11041 tagcgaacca gttttgcttg tcaaaaaaga ttctgcctac gcaagtgcta aatctttgga
    11101 tgactttaac ggtgcaaaaa tcacttctca acaaggggtc tacctttata acttgattgc
    11161 acaaatccca ggtgctaaaa aagaaacagc catgggagac ttcgctcaaa tgcgacaagc
    11221 tcttgaggct ggtgtcattg atgcttatgt ttctgaacgt ccagaagcac tgactgctga
    11281 agctgcgaac tctaagttca agatgattca agtagaacct ggtttcaaaa ctggggaaga
    11341 agatacagct atcgctatcg ggcttcgtaa aaatgacaat cgtattagcc aaatcaatgc
    11401 cagcattgaa accatttcaa aagatgacca agttgccttg atggatcgta tgatcaagga
    11461 acaacctgcc gaagctacaa caactgaaga gactagcagt agtttcttta gccaagttgc
    11521 taaaattctt tctgaaaact ggcaacaact cttgcgtggt gctggtatca ctcttttaat
    11581 ctctatcgtc ggaaccatca taggtctcat tattggactt gccattggtg tcttccgtac
    11641 tgctcctctc tctgaaaaca aagtcattta cggcctacaa aaactagtcg gctgggttct
    11701 caatgtctac attgaaattt tccgtggtac gccaatgatt gttcaatcga tggttatcta
    11761 ctatggaact gcccaagctt tcgggatcaa ccttgaccgt acactggctg ctatcttcat
    11821 cgtttcaatc aataccggtg cctacatgac tgaaatcgtc cgtggtggta tcctagcagt
    11881 tgacaaggga caatttgaag ctgcgactgc tcttggtatg acccataacc agaccatgcg
    11941 taagattgtc ctacctcagg tagtccgtaa catcctacct gcaactggta atgaatttgt
    12001 catcaatatc aaagatacat ctgtattgaa cgttatctct gttgtcgaac tttatttctc
    12061 aggaaatacc gtggcaacac aaacctatca atacttccag acatttacaa tcatcgccgt
    12121 gatttacttt gtcctcacct tcaccgtaac acgtatccta cgctttatcg agcgcagaat
    12181 ggacatggat acctacacta caggtgctaa ccaaatgcaa acggaggatt tgaaataatg
    12241 acacaagcaa tccttgaaat taaacacctc aaaaaatcct atggacaaaa cgaagtgcta
    12301 aaagacattt cactcactgt ccacaaggga gaggtcatct ctatcatcgg aagctctgga
    12361 agcggaaaat cgaccttcct acgctccatt aacctacttg aaacaccaac tgatggacaa
    12421 atcctttatc atggacaaaa cgtcctcgaa aaaggctatg acctcacgca ataccgtgaa
    12481 aagttgggga tggttttcca atcctttaac ctctttgaaa atctcaatgt tcttgaaaac
    12541 acaatcgtcg ctcagacaac tgtcctaaaa cgcgaacgca cagaagctga aaagattgcc
    12601 aaagaaaacc tggaaaaggt cggcatggga gaacgctact ggcaagccaa accaaaacaa
    12661 ctctcaggtg gtcaaaaaca acgtgtggcc atcgctcgtg ccctctccat gaatccggac
    12721 gctattctct ttgatgaacc aacatcagct ctcgatccag aaatggttgg agaagtcctc
    12781 aaaatcatgc aggacctggc tcaggaaggc ttgaccatga ttgtcgtaac ccatgaaatg
    12841 gaatttgccc gtgatgtctc tcaccgtgtt atctttatgg ataagggcgt gatcgctgaa
    12901 gaaggtaaac cagaagacct cttcaccaat cctaaagaag accgaacaaa agagttcctt
    12961 caacgctatc tcaaataaaa agaaaaggct gcatcaaccg tgcagtcttt ttgctgtcct
    13021 caaaatgaaa aggcagattc tctatctcta ctacttgaag gtcaagaatc cgccgttatc
    13081 caaagattaa tcttcaaatt tttcataatt tttttcatag aaatcaatta ggcctagggc
    13141 tgtttcaaat gaaatatttt ttacttttgc acgaccctgc gccagagcaa tgatagacat
    13201 ttcacgcgca ttcgtttctt tagagatacg gtagcctgtg attttcttat cacgaaccca
    13261 gccaacaact gattctactt tttcaaagtt tgacttagcc atgtttttct cctattttct
    13321 tctttataat atttaattgt atacgttttt actacttttg tcaataaaaa aaacatata
//
LOCUS       DAVBNO010000048        13176 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVBNO010000048 DAVBNO010000000
VERSION     DAVBNO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.048"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            276..890
                     /locus_tag="VQ846_001613"
     CDS             276..890
                     /locus_tag="VQ846_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET3076157.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFALILSSVVTGKVG
                     SLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAILRLVF"
     gene            994..1824
                     /locus_tag="VQ846_001614"
     CDS             994..1824
                     /locus_tag="VQ846_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET3076158.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            1978..2832
                     /locus_tag="VQ846_001615"
     CDS             1978..2832
                     /locus_tag="VQ846_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET3076159.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIGDTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            2932..4305
                     /locus_tag="VQ846_001616"
     CDS             2932..4305
                     /locus_tag="VQ846_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET3076160.1"
                     /translation="MFFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLCGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4298..5359
                     /locus_tag="VQ846_001617"
     CDS             4298..5359
                     /locus_tag="VQ846_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET3076161.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5361..6053
                     /locus_tag="VQ846_001618"
     CDS             5361..6053
                     /locus_tag="VQ846_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3076162.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6083..6652)
                     /locus_tag="VQ846_001619"
     CDS             complement(6083..6652)
                     /locus_tag="VQ846_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET3076163.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIAFFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            6787..7401
                     /locus_tag="VQ846_001620"
     CDS             6787..7401
                     /locus_tag="VQ846_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET3076164.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7403..9049
                     /locus_tag="VQ846_001621"
     CDS             7403..9049
                     /locus_tag="VQ846_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET3076165.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGNTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRNFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9061..10347
                     /locus_tag="VQ846_001622"
     CDS             9061..10347
                     /locus_tag="VQ846_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3076166.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQAWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRIPITKQLFIQFVMDVF
                     HLFEHLKADDMTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10399..10932
                     /locus_tag="VQ846_001623"
     CDS             10399..10932
                     /locus_tag="VQ846_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3076167.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRVAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            11010..11480
                     /gene="nrdI"
                     /locus_tag="VQ846_001624"
     CDS             11010..11480
                     /gene="nrdI"
                     /locus_tag="VQ846_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET3076168.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHLYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(11773..13035)
                     /locus_tag="VQ846_001625"
     CDS             complement(11773..13035)
                     /locus_tag="VQ846_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET3076169.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         3901 a         2452 c         3007 g         3816 t
ORIGIN      
        1 tatcttattt caatctgcta tataatagtc catgctatgg ctggattctt ttcagtctac
       61 ttatactctt cgaaactctc ttcaaaccac gtcagcttca ccttgccgta gtacggttac
      121 tgacttcgtc agttctatcc acaacctcaa aacggtgttt tgagcaacct gcggctagtt
      181 tcctagtttg ctctttgatt ttcattgagt attattaaga caatatgagg agaaagatga
      241 aagaaaaagg attttaggag ggggcgcagg cagccatgcc aacggctctt ggctatgtca
      301 gtatcggcct ggcctgtggg actatcggtg cgccctatgt gacacctgtt gagatgggct
      361 tgatgagtct ctttgtttat gctgggagtg cccagtttgc catgttggca ctgattgtgg
      421 ttcaagctcc tgtggcagct attgctatga cggtttttct aatcaacttg cgtctctttt
      481 tgttgagttt acacgcatcg acttatttcc gtcataccag tctttggtac aatatcggta
      541 tgtctagtat cttgacagat gagacctatg gcgttttgat gggtgaattg gcccatacag
      601 acaaggtaaa tcctatgtgg atgcacggaa acaatcttaa cagctatgtg gcttggtttg
      661 tggggacagt agtcggaacg gctctgggtg gcctgctacc aaatccagaa atctttgcct
      721 tgattctttc aagcgtagtg acaggtaagg ttgggagcct tcctcaaatt aaatggctag
      781 acttcttagc cgtctttcca acagcttggg tagcctttcg ctaccgcaat ctagtcggaa
      841 cagttctctt tggagtggtc ttgattgcca tcttgcgttt ggtcttttaa attacccata
      901 aaaaaaactt atcacagaga tagatatcat ataatggcgt aaatgctctt tttctgttaa
      961 gattataagg tattctattt tggaggaaat gacatgaaaa aaatcgttaa atactcatct
     1021 cttgctgccc taggacttgt tgctgcaggt gtgcttgcgg cttgctcagg gggtgctaag
     1081 aaagaaggag aagcagctag caagaaagaa atcatcgttg caaccaatgg atcaccaagg
     1141 ccatttatct atgaagaaaa tggcgaattg actggttacg agattgaagt cgttcgcgct
     1201 atctttaaag attctgacaa atatgatgtc aagtttgaaa agacagaatg gtcaggtgtc
     1261 tttgctggtc ttgacgctga ccgttacaat atggctgtca acaatcttag ctacactaaa
     1321 gaacgtgcgg agaaatacct ctatgccgca ccaattgccc aaaatcctaa tgtccttgtc
     1381 gtgaagaaag atgactctag tatcaagtct ctcgatgata tcggtggaaa atcgacggaa
     1441 gtcgttcaag ccactacatc agctaagcag ttagaagcat acaatgctga acacacggac
     1501 aacccaacta tccttaacta tactaaggca gacttccaac aaatcatggt acgtttgagc
     1561 gatggacaat ttgactataa gatttttgat aaaatcggtg ttgaaacagt gatcaagaac
     1621 caaggtttgg acaacttgaa agttatcgaa cttccaagcg accaacaacc gtacgtttac
     1681 ccacttcttg ctcaaggtca agatgagttg aaatcatttg tagacaaacg catcaaagaa
     1741 ctttataaag atggaactct tgaaaaattg tctaaacaat tcttcggaga cacttatcta
     1801 ccggcagaag ctgatattaa ataatttctt gaaatcatcc attctgagta aggtgggtgg
     1861 tttctcagtt tttagggata tagttttaaa ctatatatct gactatctaa taacaatttg
     1921 tgaaatcaaa caaattgtga gatactagta cggtattatt tttaaggaga aagaatcatg
     1981 aaaatcaaaa aatggcttgg tctagcagcc cttgctacag tcgcaggttt ggctcttgca
     2041 gcttgcggaa actcagaaaa gaaagcagac aatgcaacaa ctatcaaaat cgcaactgtt
     2101 aaccgtagcg gttctgaaga aaaacgttgg gacaaaatcc aagaattggt taaaaaagac
     2161 ggaattacct tggaatttac agagttcaca gactattcac aaccaaacaa agcaactgct
     2221 gatggcgaag tagatttgaa cgctttccaa cactataact tcttgaataa ctggaacaaa
     2281 gaaaacggaa aagaccttgt agcgattgga gatacttaca tctctccaat ccgcctttac
     2341 tcaggtttga atggaagtgc caacaagtac actaaagtag aagacatccc agcaaacgga
     2401 gaaatcgctg taccgaatga cgctacaaac gaaagccgtg cgctttactt gcttcaatca
     2461 gctggcttga ttaaattgga tgtttctgga actgctcttg caacagttgc caacatcaaa
     2521 gaaaatccaa agaacttgaa aatcactgaa ttggacgcta gccaaacagc tcgttcattg
     2581 tcatcagttg acgctgccgt tgtaaacaat accttcgtta cagaagcaaa attggactac
     2641 aagaaagcac ttttcaaaga acaagctgat gaaaactcaa aacaatggta caacatcatt
     2701 gttgcgaaaa aagattggga aacatcacct aaggctgatg ctatcaagaa agtaatcgca
     2761 gcttaccaca cagatgacgt gaaaaaagtt atcgaagaat catcagatgg tttggatcaa
     2821 ccagtttggt aataagaaac agggaggtgg gagagaaaat tccacctctt gcttttgtat
     2881 agagtataga ttgtaaagaa gactattcgt tcatagaaag gtagagagaa tatgtttttt
     2941 cctagcgaac aagaacagat tgaaaaattt gaaaaggatc atgtagccca gcattatttt
     3001 gaggttttgc gtaccttgat ttctaagaaa tcagtctttg cccagcaggt tggactcaag
     3061 gaagtcgcaa actatctggg tgagattttc aagcgtgttg gagctgaagt ggagattgat
     3121 gagagctata cagcgccctt tgtcatggca catttcaaga gttcgcgtcc agatgccaag
     3181 accttgattt tctataacca ctatgacact gtgccagcgg atggggatca ggtctggaca
     3241 gaggatcctt ttacgctttc ggtccgcaat ggcttcatgt atgggcgtgg ggttgatgac
     3301 gacaagggtc atatcacagc tcgcttgagt gctttgagaa aatatatgca gcaccatgat
     3361 gatttacctg tcaatatcag ctttatcatg gagggagcgg aggaatcggc ttcaacagac
     3421 ctagataagt atttggaaaa gcatgcagac aaactctgtg gggcggattt gttggtctgg
     3481 gaacaaggga ccaaaaatgc cttggaacag ctggaaattt ctggtggcaa taagggaatt
     3541 gtgacctttg atgccaaggt aaaaagcgct gatgtggata tccactcgag ttatggtggt
     3601 gttgtggaat cagctccttg gtatctcctc caagccttac agtctcttcg tgctgcggat
     3661 ggccgtatct tggttgaagg cttgtacgaa gaagtacaag agcccaatga acgagaaatg
     3721 gccttgctag aaacttatgg tcaacgaaac ccagaggaag ttagtcggat ttatggattg
     3781 gagttgcctc tcttacagga ggagcggatg gcctttctaa aacgtttctt tttcgagcca
     3841 gcgcttaata tcgaaggaat ccagtctggt tatcaaggtc agggtgttaa gactatttta
     3901 cctgcagaag ccagtgcaaa gctagaggtt cgtctggttc cgggcctaga accgcatgat
     3961 gttctggaaa aaattcggaa acaactagac aaaaatggct ttgataaggt agaattatac
     4021 tataccttgg gagagatgag ctatcgaagc gatatgagcg caccagccat tctcaatgtg
     4081 atcgagttgg ccaagaaatt ctatccacag ggtgtttcag tcttgccgac gacagcgggg
     4141 acaggaccta tgcatacggt ctttgatgcc ctagaggtac caatggttgc attcggtcta
     4201 ggaaatgcca atagccgaga ccacggtgga gatgaaaatg tgcgaatcgc tgattattac
     4261 acccatatcg aattagtaga ggagctgatt agaagctatg agtagagata ttatcaagtt
     4321 agatcagatc gatgtgactt ttcaccaaaa gaagagaacc attacagcgg ttaaggatgt
     4381 gaccattcac atccaagaag gggatatcta cggaatcgtt ggatattctg gagcaggaaa
     4441 atcaaccctt gtacgggtga ttaacctctt gcaaaaacca tctgcaggga aaattaccat
     4501 tgacgacgat gtgatttttg atggtaaggt caccttgacg gcagagcagt tgcgtcgtaa
     4561 acgtcaagat atcgggatga ttttccagca ttttaacctg atgagccaaa agacagcaga
     4621 ggagaatgta gcctttgccc ttaaacactc tggactcagc aaggaagaaa agaaggctaa
     4681 agtagctaag ttgttggact tggttggttt ggcagatcgt gctgaaaact acccttcaca
     4741 attatctgga gggcaaaaac agcgtgtggc aattgcgcgt gccttggcca atgatccaaa
     4801 aatcttgatt tcagacgagt caacttctgc ccttgaccct aagacaacca agcagatttt
     4861 ggccttgttg caagatttga accaaaaatt aggcttgact gttgtcttga ttacgcatga
     4921 aatgcagatt gtcaaagaca ttgccaaccg tgttgcagtt atgcaggatg ggcatttgat
     4981 tgaagagggt agtgtgcttg aaatcttctc aaaccctaaa caacctttga ctcaagactt
     5041 tatctcaaca gctacaggta ttgacgaagc catggtcaaa atcgagaagc aagaaatcgt
     5101 ggaacacttg tctgaaaaca gtctcttggt gcaactcaag tacgctggag cttcaacaga
     5161 cgagccactt ttgaatgaat tgtacaagca ttaccaagta atggctaata ttctctatgg
     5221 gaatatcgaa attctcgatg gtactcctgt tggagaattg gtggtggttt tgtcaggtga
     5281 aaaagcagcg ttggcaggtg cccaagaagc cattcgtcaa gcaggtgtac aactaaaagt
     5341 attgaaggga gtacagtaag atggaatcat tgattcaaac ctatttacca aatgtctata
     5401 agatgggttg ggctggtcag gcaggctggg gaacggctat ctacttaact ctttatatga
     5461 cagttctttc cttcattatc ggaggcttct tggggctagt ggcaggtctc tttctcgtct
     5521 tgacagcgcc aggtggtgtc ttggagaata aagtcgtatt ctggatttta gacaaaatta
     5581 cctcaatttt tcgtgcggtt ccctttatca tcctcttggc aatcttgtca ccactttctc
     5641 acttgattgt taaaacaagt atcgggccaa atgcagccct tgtcccactt tcttttgcag
     5701 tctttgcctt ctttgcccgt caggtgcagg ttgtcttggc tgaattggat ggcggtgtca
     5761 ttgaggcggc tcaagcgagc ggagcgactt tctgggacat cgtgggtgtt tacctatcag
     5821 aaggtcttcc agatttgatc cgtgtgacga ctgtgacctt gatttccctt gttggggaaa
     5881 cagctatggc cggtgcggtt ggagctggtg gtatcggtaa cgtagccatc gcttatggat
     5941 ttaaccgcta caatcacgat gtgaccatct tggcaaccat tattatcatt ttgattatct
     6001 ttgcaatcca attcttggga gatttcttga ctaagaaatt gagccataaa taaaaaagag
     6061 ccgcgtggct ctttttaact gatcagattt tctgtgcaaa ttttttactc aaggcttgtc
     6121 caatcaaagc acccactagg gctccgatga caatacttgc gataaataga aggacagttc
     6181 cagggtttgg agcgaccatg atacggtcga tatattcttg ggattttcct cttgccagaa
     6241 gagtagccat ataggctttg ggcgcaatcc acataagcaa gattggtcct gttgtactaa
     6301 aggcgaaaat aatgaaagaa aggaagttct ttgttttgtc cttgtatttt cctaaatgag
     6361 ctacttcatc tgctaggagg ccacagataa ttccaggaag gaaggaacca gcaccgtgtt
     6421 tagttcccaa gaaaaagaag gcaatgacaa ggccgatagt ggtaatggct ccaaagcgcg
     6481 gaacttttgc gactaggatc atatagacgc taccgccgac aagggcagta aaggcaggcg
     6541 cataaaacat gtttccagtt tggtcaaaga gattgcccaa aaggacacca atccccatgc
     6601 agaggaagta aagaactgca aaaagcagtg tagttaagat agatttcttc atgaattgtc
     6661 tcctgataat tttttcacaa ttctcatttt accatggttt ggtgggattg taaatggata
     6721 ctagaagttt ttgaaaacgc ttgaaatatg atataatggt ttcatagtta tttttagggg
     6781 aatatcatgg gtagattttt agactttgtc tttaatcgtt tctttttagg gatgattgcg
     6841 acagccttct tttggctatt aactttagca ggagggatta tccttggtct agcgccggct
     6901 agtgccacct tgatgagctt atatgcagaa catggttata gctttcggga atacagtttg
     6961 aaggaggctt ggtctcttta caagcaaaat tttgtctcaa gcaacctgat tttctatagc
     7021 tttttaggtg tgggtctagt tttgacctat ggtttgtatc tcttggtgca attgcctcat
     7081 cagaccattg ttcatttgat tgcgaccctt ttgaatgtct tagtagttgc cctgatcttt
     7141 ttggcttata cagtatcttt aaaattacaa gtttattttg ccttgtccta tcgaaatagt
     7201 ctcaaattat ccttgattgg catctttatg agtctagcag ctgtggccaa ggttctcctt
     7261 gggactgtgc tacttgtagc aattggttac tatatgcctg ccctgctatt ttttgtagga
     7321 attgggatgt ggcatttctt tatcagtgat atgttggaac ctgtctatga aatcatccat
     7381 gaaaaattgg cgacaaaata gaatgaagca gttttggcta catacgcttc taagaaccta
     7441 tagttcagtg atgatcatta tcattgcgag ttttgcaatc ttactctctt acgctgactg
     7501 ggattcacgt gaaaaggaag cccagagagt agcccagcgt gtaactgcta gaacagtgag
     7561 tgaaattgaa tattaccata gagagtcaac ccagatagct caggctttag ttgaaaatca
     7621 agctcgtatt gagggaatct ataaatactt tagccttagc atgccagact atttttactg
     7681 gcaattagag cggaaagctt cgccttatat atcagtctct ctgtatgaaa atgttgatga
     7741 cctctatgtt cgaaatgatt ttgtaactgg agtggccatt gcttttcaag attacaagga
     7801 agtctatgtt tctactaaag acaaacgtag tggagaaaaa atcagggctg aggatttcaa
     7861 accagcagga aatagttttg ccattccggt gtcagatcca gtgtcagatc aagatttagg
     7921 agtgatttac atctccttgg atcctgctgt tttataccat gccattgata atactagagg
     7981 taatactccg atggcagtaa cagtgacctc accttttgat acggagattt ttcatattgg
     8041 tgagacagtt gataaggaga gtgaaaattg gctagttggc ttaacttctc atggatatca
     8101 ggttcaggtg gcagttccta aaaactttgt tttacaagga acagtgacta gctctgcttt
     8161 gattgtgggt ttgagccttc tctttattgt cattctttat ctgactttga ggcagacttt
     8221 tgctaattac caaaagcagg tagtggattt agtagaatcc attcaagtca ttgctcaagg
     8281 cgaagagggt cgtcggattg acatttccga gaaagatcag gaattactcc taatcgcgga
     8341 gacgaccaat gatatgttgg atcgattgga aaagaatatc catgatattt accagttaga
     8401 gcttagtcaa aaagatgcta atatgcgagc cttgcaggcg caaatcaatc ctcattttat
     8461 gtataatacg ctggagttct tgcgcatgta tgcagttatg cagagtcaag atgagttggc
     8521 agatatcatt tatgaattca gtagtctctt gcgtaacaat atttccgacg aaagagagac
     8581 cctcctcaaa caggaattag aattttgccg taaatacagc tatctctgca tggttcgcta
     8641 tcccaagtcc attgcctatg gtttcaagat agatccagag ttagagaata tgaagattcc
     8701 taagttcacc ttgcaaccgc tggtagaaaa ttatttcgcg catggtgttg accacaggcg
     8761 gacagataat gtgattagca tcaaggctct taaacaggat ggttttgtgg aaattttggt
     8821 ggtcgataat ggtagaggaa tgtcggctga aaagttggca aatatccgag aaaaattaag
     8881 tcagagaaat tttgaacacc aagccagcta cagtgatcaa aggcagtcta tcgggattgt
     8941 caatgtacac gagcgttttg tgctctattt tggagaccgc tatgccatta ctatagagtc
     9001 tgcagagcaa gccggtgttc agtatcgtat tacaattcaa gatgagtaga aagggagaaa
     9061 atgtataaag tattattagt agatgatgag tacatggtga cagaaggtct gaagcgtttg
     9121 attccctttg ataagtggga tatggaggtc gtcgcaacag ccagtcatgc cgatgaagct
     9181 ctagaatatg ttcaggaaaa tcctgtcgat gtcatcattt ccgatgtcaa tatgccagac
     9241 aaaacagggc ttgatatgat tcgggagatg aaagagatct taccagatgc tgcctatatc
     9301 ctgctctcag gttatcagga gtttgattat gtaaaaagag caatgaacct tagtgtggtg
     9361 gactatttgg tcaaacctgt tgataaggta gagctgggaa atctgctgga gaagattgca
     9421 ggtcagctcg gcgagagagg gaagaaaagt cagactctta gtcaagaatt agacgaggct
     9481 ggatttgtta gttatttagg ggataaggag aattggtgga taggtctatc caaggaaaaa
     9541 caaggttcct tcaccattcc ctactatgtc ttgggtcaag cctggcagat tttcatttct
     9601 gaccaacccc tagatggttt agtcgttaca ccttttgaag ctccttatca agaacatttt
     9661 gaacgctgga agctgaatgc tgagaaaacc ctcttttacg gttctgtaaa tctgcagcag
     9721 tctgagagtc tctttgccta ttacgaaccg atttataggg ttatcattca gggaaatctc
     9781 aatcaaatcg tagaagagtt aaatctcttg gagaaggtag ttcttgaaaa tacgccgcga
     9841 attccgatta ctaaacagct ttttatccag tttgtcatgg atgtcttcca tttatttgaa
     9901 catctcaaag ctgatgatat gacggacatt gtcaaaacca ttcatgctat tcaatccttc
     9961 gatgaattgg tttcttatat caaggaaact ctgatcagct ttttcggtca ataccgtatg
    10021 aatgaaaatg tggtcagtgt gctggaagtc attggtcgtg attaccaaaa agagctttcc
    10081 ctcaaggata tcagtaaggc cctctttatc aatcctgtct atctagggca gttgattaag
    10141 cgtgaaaccg attcgacctt tgcagagtta ctaaacaaac aacgtattaa ggctgcccag
    10201 cagctcttgc tttcaaccag tgacagtatc gaagatattt gttatgctgt tggttacagt
    10261 aaccttggat atttctataa agttttccga aaattgtgcg gaaaatcgcc aaaagcctac
    10321 cgaaaacagg tagaaactat actataagat ttgtattcct ttacaaaatg tgctataata
    10381 ttatcaggag gttctatcat gaaaaagaaa ccaatttatc tatgggtctt gctaatcttg
    10441 tctgccctta tttcagttcc gtctctgttt ggaattgtga gtcccttgcc tagtaaagaa
    10501 gcccttcgtg ttgctcagaa acaagttgca ggggtcaatg ctcagcaatt agaagaccag
    10561 cttaattata cctatagagt agcagaagca tctcattcta tttttaatgt tgctttgatt
    10621 gtgctatcta ctattttagt ggtcgtagcg attgtcttcc ttgttcgtaa aaatttgcaa
    10681 tatgcaaact atacttacgt tggctatgtt ttgttagcta ttattggttc gatttatggc
    10741 tatgttggtt tacaagacgc tgtgcagttg gttcaagatg agagcatgcg tttgacagtg
    10801 agtattggtt caaaagctgt tagcattttc tatatcgtca tcaatgttct cttcctagcc
    10861 cttgtcttct ataagatgtg gcgacaacag aaagccttgg cagaagaaga ggaaacagaa
    10921 gagcttgcct aagtattgac aaaaaagaac tatcaagata caatcttggt agttattttt
    10981 tgattataga taaaatcatg gaggtacata tgaagacaat ttctttagtt tatattagtt
    11041 tgagtggcaa cactgaaagt tttgtgacgc gcttgaaaga ctatctcttg tcccagtaca
    11101 aagggattga ggttcaaaag attcatatca aggatttggt caaggaaggc aaaaatttct
    11161 atgaaatgga ccatctctat gtcgcttttt tgccgaccta tctcgaaggt gggaatggcg
    11221 tggataacgg agatgttgag attttgacaa caccggtggg agattttatt gcctatggta
    11281 acaatgctag taagtgtttt ggtgtggttg ggtcaggaaa tcgtaacttt aataaccaat
    11341 actgcctaac agccaagcaa tacagtcaac gctttggctt ccctgtattg gctgactttg
    11401 aaatgcgagg catgctggaa gatatcaaac atgtcgcagc tattatcgca gatttgtatg
    11461 agttggaaaa ggagaattaa tactcaatga aaatcaaaga gcaaactagg aagctagtcg
    11521 caggttgctc aaagcacagc tttgaggttg cagatagagc tgacgtggtt tgaagagatt
    11581 ctcgaatagt ataaagttct tataagaggt ggttgaagtt gttcaactgt tttttgagta
    11641 taaacagtct ttaagaacgt aaaaaagcat aagttcaggt cttcaaaacc ttgttcttat
    11701 gctttttatc atagaaatat ttgctgaatt ttctcttgaa atgaaacctt atcttcatct
    11761 cgcgcttgtc ccttaccctg cttttccgat tgcgagtgcg tagataaaga agatggcgaa
    11821 gccaaagagg aaaatcaatc ccgcaatggc aaggtgtttg agccaagaag gaagattttt
    11881 gttttcacgc gttaccaagg cgtaattcaa aagagcaaag aaaggtgttg tcaggaaaga
    11941 tccaatcatg gcaaagcgga gcatggttga aacctgacca gcgaagaact tgataatgac
    12001 gataccgatg atagcagtga tggtcatcca gatgttcaaa gatttacgat tgtcctcttt
    12061 ttgactgatt agcagtcgga gagattcctg attaacgcga gaatagccat cgataacagt
    12121 tataactgtt ccaaagatac agaggaaggc aataaaggta atcaagtaac gggaccattc
    12181 gccaagaaca gaggcataca tgcccacgaa ttgagagatg tatttggctg aagcagcttc
    12241 aaccgcctgc cctgtaggat actgaatcag tgctcccagt gccacaaaga agacggctag
    12301 gatagctgtt ccaatataac cagtgttaaa gtcaaacaga gcgtcttctg tgttaaagtt
    12361 gacggtcttt ctcttttcag ctgaccaaag tgaattgata gctgaaattt caataggagc
    12421 cggcatccat cctaggaggg agacgatgaa gggcagagct gccatttgcc aaggtgtctt
    12481 ctcgacaaaa tcagaactgt attctggatg cttgaccgct gctatgataa ctgccagaac
    12541 agtcgcaatg gttaaggcag acataatcca tttgaccatg ccgtctaaaa gtttgtagcc
    12601 tccaaagagt agcatagccc aaatgattgc aacgagaatg agggaccact gagtaatgct
    12661 aagtccaatc attgggaagg cactggcgat gatagctgag cacagaatgg caacaccagc
    12721 cgtgttgacc atagccgaaa agacattgag gataaagaaa atccagagat agagttttcc
    12781 tttttcggca taaccttcaa ccaaagtctt tccagtatca gctgtgtatt cagcaccaaa
    12841 acggaaaaat ggatatttaa agacattggc taagatgact aagagaagta gagaccaacc
    12901 gtaagaaccg ccagcttgag ttgaggatac aatgtgggaa cctccaacag cggcagttgc
    12961 cattaggatt ccaggtccca ttgcttttag cttgcttgcc caggttgatt gatttaagga
    13021 aatagcttgc gacatgataa atactccttt ttgaattttc agaataatct gtatatgtaa
    13081 tctatattct acaaaaaatt ttggaaaaat gcaagaatat tttgaaaaaa gatagaaaat
    13141 ttacaaagaa aatctgaaat aactgttatg atatag
//
LOCUS       DAVBNO010000049        13114 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVBNO010000049 DAVBNO010000000
VERSION     DAVBNO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13114
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.049"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..367
                     /locus_tag="VQ846_001626"
     CDS             <1..367
                     /locus_tag="VQ846_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET3076170.1"
                     /translation="PNLEQVQTMLEQTFTEKHYENTILHSDQGWQYQHDSYHQFLESK
                     GIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKSFQSRKQLEQAIVDYIDYYNNKR
                     IKVKLKGLSLVQYRTKSFG"
     gene            complement(442..972)
                     /locus_tag="VQ846_001627"
     CDS             complement(442..972)
                     /locus_tag="VQ846_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HET3076171.1"
                     /translation="MLDEKRKNYVEDKRQNFPSLPSWKMDLFSEEEKRIYFQTTCEVS
                     SKDSAINKYKIEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDAEGEWTALDDI
                     KNEAYMLKKLADKSYTTNLTDEFYIVDDYFLVQEQVDGLNFEEFIRETEHSLNIREKT
                     LDNIVNIVSDIHKLGI"
     gene            complement(966..1434)
                     /locus_tag="VQ846_001628"
                     /pseudo
     CDS             complement(966..1434)
                     /locus_tag="VQ846_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1984..2661
                     /locus_tag="VQ846_001629"
     CDS             1984..2661
                     /locus_tag="VQ846_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET3076172.1"
                     /translation="MISKRLELVASFVPQGAILLDVGSDHAYLPIELVERGKIESAIA
                     GEVVEGPYQSAVKNVEAHGLEEKIQVRLANGLAAFEEEDRVSVITIAGMGGRLIARIL
                     EEGLDKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            2648..3445
                     /locus_tag="VQ846_001630"
     CDS             2648..3445
                     /locus_tag="VQ846_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET3076173.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            3462..4565
                     /locus_tag="VQ846_001631"
     CDS             3462..4565
                     /locus_tag="VQ846_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET3076174.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARATSRAERVGIRAYTSDFSPFFGQVPELAGVYVASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            4821..5840
                     /gene="galE"
                     /locus_tag="VQ846_001632"
     CDS             4821..5840
                     /gene="galE"
                     /locus_tag="VQ846_001632"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET3076175.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTVTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            5842..6795
                     /locus_tag="VQ846_001633"
     CDS             5842..6795
                     /locus_tag="VQ846_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET3076176.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(6857..7063)
                     /locus_tag="VQ846_001634"
     CDS             complement(6857..7063)
                     /locus_tag="VQ846_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET3076177.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            7115..7591
                     /locus_tag="VQ846_001635"
     CDS             7115..7591
                     /locus_tag="VQ846_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET3076178.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            7600..8271
                     /locus_tag="VQ846_001636"
     CDS             7600..8271
                     /locus_tag="VQ846_001636"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET3076179.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            8402..8740
                     /locus_tag="VQ846_001637"
     CDS             8402..8740
                     /locus_tag="VQ846_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET3076180.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            8743..9372
                     /locus_tag="VQ846_001638"
     CDS             8743..9372
                     /locus_tag="VQ846_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET3076181.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            9612..10763
                     /locus_tag="VQ846_001639"
     CDS             9612..10763
                     /locus_tag="VQ846_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET3076182.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            11081..11830
                     /gene="truA"
                     /locus_tag="VQ846_001640"
     CDS             11081..11830
                     /gene="truA"
                     /locus_tag="VQ846_001640"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET3076183.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQIDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IALILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            11820..12593
                     /locus_tag="VQ846_001641"
     CDS             11820..12593
                     /locus_tag="VQ846_001641"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET3076184.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LVKGDKLLPAVESSKVFVYRAIAQADQYGVRQYEANKNN"
     gene            12571..13032
                     /locus_tag="VQ846_001642"
     CDS             12571..13032
                     /locus_tag="VQ846_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET3076185.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLLDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMAGMIVGFILSQSIKKIK"
BASE COUNT         3748 a         2459 c         2893 g         4014 t
ORIGIN      
        1 acccaactta gaacaagtac aaacaatgtt ggaacagaca ttcacagaga agcactacga
       61 gaatacgatt ctccatagtg accaaggctg gcaataccaa cacgattctt atcatcagtt
      121 cctagagagt aagggaattc aagcatccat gtcacgtaag ggcaacagcc cagacaacgg
      181 tatgatggag tccttctttg ggattctgaa atcggaaatg ttttacggtt atgagaagtc
      241 gtttcagtcg cgtaagcaat tggaacaagc tattgtagac tatattgatt actacaacaa
      301 taaacgaatt aaggtaaaac taaaaggact tagccttgtg caatacagaa ctaaatcctt
      361 cggataaatt aattgtctaa ctttttgggg ggcagtacat ttttggtata tataaaattt
      421 gtaggagcta tatctacaat tttatattcc cagtttatgg atgtcgctta ctatgttcac
      481 aatgttatcc agtgtttttt ctctaatatt taaggagtgt tctgtttctc gaataaattc
      541 ttcaaagttt aacccgtcaa cttgttcctg aacaagaaaa taatcatcca cgatataaaa
      601 ttcatcagtt aaattagtag tataactttt atcggctaat ttttttagca tgtaagcctc
      661 attttttata tcatcaagtg ctgtccattc tccttcggca tcataattca caaaaggtct
      721 tgactgcttg atgattactt tttgcccgtc cgattttcta attgcccgat aaacattccc
      781 tttatttgat ctcttaataa ttttttctat tttgtattta tttattgcag agtccttact
      841 tgaaacttca catgtggttt gaaaataaat ccttttttct tcttctgaaa ataaatccat
      901 tttccagcta ggaagactag ggaaattttg tctcttatct tctacatagt tcttcctttt
      961 ttcatctagc aataaataga tgactttttt atttttttca tcataagctt gtttttttaa
     1021 aaaagcccca tatctataat gaactggaga atgcattcca cattgatagt cagatagtat
     1081 ttttggagcc ttaaattctg acagtctatt cgtaagatta caaatcatac tcttagcttc
     1141 agattctgac ttaggatata gagttataaa tttgttagca gtagggctca tttccctagg
     1201 ggaattaatt ctttttaatt cctctaaatt tttaacaact ttaaagctac aattatttag
     1261 ttgggatagt ttatacacaa tcttaaaaat atttacagcg tcttttattt gggagcttat
     1321 gtgtattttc cacccttgtg caggcacctt atctgataaa taatagtaat tccaaatatc
     1381 accctgttgg aaatttttat cacattttaa acattctttc tctaataaat ccataacaaa
     1441 ctcctaatta ttaaaatagg ctagagacac agcactagcc tattccgtaa gtaaagctac
     1501 ttattttata taaaatataa ctagcgacct gtaattaaac aagtaaaaga acaatactta
     1561 gggagacctg tgacaatgat atcttcctct ggaagtagtt ctaattcaaa caatgtttgt
     1621 tccatgactg tatcctcctg ttatgataaa ttccagaaag catagagcaa attcgtggtt
     1681 cgtctgacta aatttcttat ctgaacacca ccttcaaata caaattttag tcaaactttt
     1741 attttttcta actttctaat tatattctac catgtgtttt tatatgtcaa gaattttctg
     1801 aataaactgt ttatttttta tttttttgac atttttgcaa attgtttaaa atctttcaat
     1861 tggattcact tattacttta ggacattgaa gcttctttcg ccagaggtgc ttatattact
     1921 gggaagttga agattgaaat gtggtataat aagaggtaat agagttttgg aaagagagag
     1981 aagatgattt caaagagatt agagttggta gcttcctttg tgccgcaggg agccatttta
     2041 ctagatgtgg ggagtgacca tgcttatttg cctatagagt tagtcgaaag aggaaaaatc
     2101 gaaagtgcca ttgcaggtga agttgtggag ggaccttatc agtctgctgt caaaaatgtt
     2161 gaggcccacg gcttggaaga aaaaatccaa gtccgtttag ccaatggttt ggcagctttt
     2221 gaagaggaag accgagtgtc tgtcattacc atcgcaggga tgggcggccg tttgattgct
     2281 aggattttag aagaaggctt ggacaagtta gctaacgtag agcgtttgat tctccagccc
     2341 aataatcgtg aagacgactt gcgtatctgg ctacaggatc atggattcca gattgtagca
     2401 gaaagcatct tagaagaagc tggaaagttt tatgagattt tggtggtgga agcaggacaa
     2461 atgaagctat cagccagtga tgttcgcttt ggtcccttct tgtccaaaga agtcagtcca
     2521 gtatttgtcc aaaaatggca aaaagaagct gagaagctag agttcgccct cggacaaatc
     2581 ccagaaaaaa atctggaaga acgtcaagtt ctagtagata agattcaagc tatcaaggag
     2641 gtgctccatg ttagcaagtg aagtgatcca agcttatgaa gccttttgcc ctcaggaatt
     2701 ttctatggag ggagacagcc gtggtctgca aattggcact ttagacaagg gtatccaaag
     2761 ggtcatggtt gccctcgata ttcgtgaaga gacagtggca gaggccattg aaaagggtgt
     2821 ggacttgatt atcgtcaagc acgcgcctat ctttcgtcct atcaaggact tgcttgccag
     2881 ccgtccgcaa aatcagattt acatcgacct gattaagcat gacatcgcag tttatgtcag
     2941 ccataccaat attgatatcg ttgaaaatgg gctcaatgac tggttttgtc agatgctagg
     3001 aatcgaggag acgacttatc tgcaggaaac aggtccagaa cgtggaattg gacgtattgg
     3061 gaatattcag cctcagacat tttgggaatt ggcccaacag gtcaagcaag tctttgacct
     3121 agatagcctt cgaatggtgc attatcaaga gaatgatttg cagaagccta tttcaagagt
     3181 agcaatttgt ggtggaagcg ggcagtcttt ctataaggat gctttggcaa agggggcaga
     3241 tgtctatatc actggtgata tctactacca tactgctcag gatatgctgt ctgatggctt
     3301 gttagcattg gatccaggtc actatatcga agtgcttttt gtggaaaaaa tcgcagcact
     3361 ccttactcaa tggaaagaga aaaaaggctg ggaactagag attttaccta gtcaagcatc
     3421 gaccaatcct ttccgtcata tctagttaga aagtgaagac aatgaaaaaa gttgccatta
     3481 ttggagcagg aattgtggga gcaacagctg cctactacct ctcgagagaa agtgacctag
     3541 aggtgaccgt ttttgaccat gagcaaggtc aagccaccaa ggccgcagca ggaattatca
     3601 gtccttggtt ttccaaacgc cgtaataaag cctggtacaa gatggcgcgc ttgggggctg
     3661 acttttatgt ggatttatta gctgatttag agaaatcagg acaaaaaatc gacttttacc
     3721 agcgttcggg agtctttctc ttgaaaaagg atgaatccaa gttggaagaa ctttatcaac
     3781 tggccctcca gcgcagagaa gaatctccct tgatagggca attagccatt ctggaccaag
     3841 cttcagctaa tgaattattc cctggtttgc agggatttga ccgcctgctc tatgcttctg
     3901 gtggagcgag agtagatggc caactcttgg taactcgtct gctagaagcc agtcatgtca
     3961 agctggtcaa agaaaaagtg actctgacac tcttatcatc aggctaccag attggcgaag
     4021 aggtgtttga tcaggttatt ttggcaacgg gagcttggtt gggggacatg ttagagcctt
     4081 taggttatga agtggatgtc cgtcctcaaa aaggacaact acgagattat cagcttgccc
     4141 aagacatgga agattaccct gttgtcatgc cagaagggga gtgggatttg attccctttg
     4201 caggtgggaa attatccttg ggcgctaccc atgaaaatga catgggattt gatttgacgg
     4261 tagatgaacc cttgctccaa caaatggagg aggcggcctt gcctcattac ccagttttag
     4321 ctagagcgac ttcaagggct gagcgtgtgg gaatccgtgc ctataccagt gatttctctc
     4381 ccttctttgg gcaggtgcca gaattggcag gtgtctatgt agccagtgga ctaggttcat
     4441 caggcctcac aactggtcct atcattggtt accatctagc ccaactgatc caagacaagg
     4501 agttgacctt ggaccctcta aattacccaa ttgaaaacta tgtcaaacga gtaaaaagcg
     4561 aataatactc aatgaaaatc aaagaacaaa ctaggaaact agccgcgggt tactcaaagc
     4621 actgctttga ggttgtgtat agaactgacg aagtcagtaa ccatacccat ggcaaggcga
     4681 cgttgacgtg gtttgaattg gattttcgaa gagtataagc attttactga aattttatca
     4741 ggtagtttag gatggcaaat gacattccct gtcaaaaatg gtaaaataag aaaaaataat
     4801 ctgagaatcg aggaaaaaag atgcaagaaa agattttggt aactggtggt gccggtttta
     4861 ttggaaccca cactgttatt gaattgatcc aagcaggcca tcaagttgtt gtggtggata
     4921 atcttgtcaa tagcaatcgt aagagtttag aagttgttga aagaatcaca ggagttgaga
     4981 ttcctttcta tgaggcagat attcgtgaca cagttaccct cagagatatt ttcaagcagg
     5041 aagaaccgac tggtgtcatt cactttgctg gtttgaaggc tgttggcgaa tcaacacgta
     5101 tccctcttgc ctactatgac aacaatatcg ctggaactgt cagccttttg aaagccatgg
     5161 aagaaaacaa ctgtaaaaac atcatcttca gttcttctgc gacagtttac ggggatccgc
     5221 acacagtgcc catcttggaa gatttcccac tttcagtgac caacccatac ggtcgtacca
     5281 agctcatgct agaggaaatt ttgaccgata tttacaaagc agactcagaa tggaatgttg
     5341 tcttgcttcg ttactttaac ccaatcggag cccatgagag tggtgatttg ggagaaaatc
     5401 caaacggtat tccaaacaat ctcttgccat atgtgactca agtagccgtt ggaaaattag
     5461 agcaagtgca agtgtttgga gacgattacg atacggaaga tggaacaggt gttcgtgact
     5521 atatccacgt tgtcgatttg gctaagggtc acgttgcagc tttgaaaaaa atccaaaaag
     5581 gttcaggact aaacgtttat aaccttggaa ctggtaaagg ttactcagtt cttgaaatta
     5641 tccaaaacat ggaaaaagcg gtgggacgtc ctattcctta ccgcatcgta gaacgtcgcc
     5701 caggtgatat cgctgcctgc tactcagacc cagcaaaagc taaagcagaa ctcggttggg
     5761 aagcagaact cgacatcacc caaatgtgtg aagacgcatg gcgttggcaa agcaagcatc
     5821 caaatggatt tgaagactaa gatgatgatt tcaatcatcg tcccttgttt aaacgaagag
     5881 gaagtacttc ctctttttta tcaggctctg gaagctttac ttccagattt ggaaacagaa
     5941 atcgagtatg tctttgtcga tgatggatca agtgatggga ccttggaact cttaaaggcc
     6001 tatcgggagc aaaatccggc agtccattat atttctttct ctcgaaattt tggcaaagaa
     6061 gcagcccttt atgcaggctt gcaatatgcg acaggagatt tggtggtggt gatggatgca
     6121 gacctccaag atcctcctag tatgttgttt gagatgaaaa atgtactaga caaaaatgta
     6181 gacttggact gcgttgggac acggagaact agtcgggagg gagaaccctt ctttcgcagt
     6241 ttctgtgctg ttctctttta tcgcctcatg caaaaaatca gcccagtagc tctgccgtcg
     6301 ggtgtccgtg attttcgtat gatgagaagg tctgtggtcg atgccatttt aagcttgact
     6361 gagtccaatc gtttttctaa gggactcttt gcctgggtcg gctttaaaac ccactatctg
     6421 gactatccaa atgtcgaaag gcaggctggc aagaccagtt ggagttttag gcaactcttt
     6481 ttttactcca ttgaagggat tgttaatttt tcagatttcc ctttgactat agcctttgta
     6541 gctggtctcc tatcttgttt tctttctctg ctgatgacct tttttgttgt ggttcggacc
     6601 ctcattttgg gcaatccgac atctggttgg acctctctga tggctgttat tctctttctt
     6661 ggaggcattc aactcttgac cattgggatt ctcggtaagt atatcagtaa gatttattta
     6721 gagactaaaa aaagaccact ttatcttatc aaagaaaaaa gtgaccttcc tgattttaca
     6781 gaaaaaataa agtgaaaaga ctataatttt acatggaaat gtgctaaact agagggagtg
     6841 gattatcgcc attgatttag gcttcctggt ttccaatcag ggcgcgagtt gggcaatttt
     6901 taacagcctc taagacatcc tgactaggag aaatttcttt ttccagttgg tcagggtcat
     6961 cgtaaaaacg cacgattcca ttatcgtggt aatcaaataa atcagaataa gtttggcaaa
     7021 gtccacaggc gatacatcgt tcaggtataa gtgtgatttt catgttcata tttatattgt
     7081 aataagaaaa gtaaaaaaaa acaaggagta aggtatggca aaagaaccgt ggcaagaaga
     7141 tatttatgat caagaagaat caagagcagg gcgtcggcat cgaaaccacg gaggggctga
     7201 taggatggct aatcgtattt tgacgatcct agctagtatt ttctttgtaa ttgtggtggt
     7261 gatggtcatc gttctcatct atctatcatc gggggggagt aatcgcacag cagccttaaa
     7321 agactttcat gattctgatg caagtgtagt acaaatctca tcttcaagca gttctcagcc
     7381 tgagcagagt tcagagccag aatctacttc tagtagttca gaagaagctg ctaatcctga
     7441 aggaacgatt aaagttctcg caggagaagg ggaagcagct attgccgctc gtgcaggaat
     7501 ctccattgct cagttagagg ccttgaatcc tgggcacatg gctacaggat cttggtttgc
     7561 taatccaggt gatgttataa aaataaaata ggagccgata tgaaaacaat tcaaattgct
     7621 attgatggtc ctgcttccag cggtaagagt acggtcgcaa agattattgc taaggatttt
     7681 ggattcacct accttgatac aggagctatg tatcgtgcag cgacctatat ggctcttaag
     7741 aaccaattag gagttgaaga agtcgaagcc cttctagcct tgttggacca gcatccaatc
     7801 agctttggac gttcagaaac tggagaccag cttgtttttg taggagatgt ggatattacc
     7861 catcctatcc gtgaaaatga agtgaccaat catgtttctg ctattgcagc aattcctgaa
     7921 gtgcgtgaga aactggtttc tctccaacaa gaaattgccc agcaaggcgg gattgtcatg
     7981 gatggtcgcg atattggaac tgttgtattg ccacaagcag aattgaaaat tttcctagta
     8041 gcttctgttg atgagagagc agagcgtcgt tacaaggaaa atattgccaa gggaattgaa
     8101 acagaccttg aaaccctaaa aaaggaaatt gctgcgcgtg actacaagga tagtcatcgt
     8161 gagacttctc ctctcaaaca agcagaggat gctgtctacc ttgatacaac tggtttgaac
     8221 attcaagaag tagttgaaaa aatcaaagca gaagctgaaa aaagaatgta aatgtagaaa
     8281 agccgataga ttgatatcgg tttttttcta aatttgtcaa aatgctgatt ttagggtata
     8341 atagttgctg ttcgagaaat tttgattttc aaagaatagt gaatgatgat aaggagaaac
     8401 catgaacaat ttaccaaatt gtcccaaatg taactcagag tatgtctacg aagacggtgc
     8461 cctactggtt tgcccagagt gtgctcatga gtggaatcct gctgaagttg cagaagtaga
     8521 agaaggtctt gtcgctatcg atgccaacgg aaataaattg actgatggtg atacagtaac
     8581 tctcatcaag gacttgaaag taaaaggtgc gccaaaagat ttgaaacaag ggacgcgcgt
     8641 gaaaaatatc cgcatcgtag aaggcgacca caatatcgac tgtaagattg atggttttgg
     8701 tgccatgaaa cttaaatcag agtttgtgag gaagatttaa gcatgtcaaa acactataaa
     8761 cttgtatttt atagccgtat cttcttgttt ctagcggctt ttacgggagt ttatcttgaa
     8821 attactaggc atggtggttt tgggatgctt ctctattaca cggttctgtc caacctcttg
     8881 gtaactattt ttacccttta tcttctaaag gttatgagcc gtgtaggtga aaattggcaa
     8941 aggccaagtc tcttgcgctt aaaaggtggg gtcaccatga gcatcatgat tacctgtgtg
     9001 atttaccatt tcctcttggc gcccattgcg actaatttct ataccctaga aaatttcctt
     9061 tgccactata tcgttcccat ctggttttta gcggataccc tcttttttga caaacagggt
     9121 caatacaaga tttgggatcc agcagtgtgg accattttac cctttctgta tatgatgttt
     9181 gctcttttta atggcttggt tctaaaactc aatattccaa atgccaagga taatcctttc
     9241 ccttacttct ttttgaatgt gaacaagggt tggaatgttg tgtttaagtg gtgtctgatt
     9301 atctttgttg cctatatggt agcaggattt attttctact ttatcaagca aatcaagaga
     9361 aagtcatcct aatactcttc gaaaatctct tcaaaccacg tcagtttcca tctgcaacct
     9421 caaaacactg ttttgaacaa cctgcggcta gcttcctagt ttgctctttg attttcattg
     9481 agtataagat acaagggatt cattttcgaa tttaagaagg agtctacaaa ccaatgaggc
     9541 ttcttctttt cttgcttatt tgataactat caaaaaagta gaagattaag agaaatatag
     9601 aaagagattt catgaaacaa tttttagaac gggccagcat tttggctctc tccctcgttt
     9661 tgattacctc cttttccatt tcgagtgccc taccagccat gtttgactat tatcagggtt
     9721 attctaagga acaaattgag ctcttggtga gcttgccttc ctttggaatt atgatgatgt
     9781 tactgctaaa tggtttctta gaaaaaatat ttcctgagcg cttacagatt agtttgggct
     9841 tgctgatttt atcattgagc ggtacagctc ccttctggta ccaagcctat ccctttgtct
     9901 ttggaacacg gcttctcttt ggtttgggtc ttgggatgat caatgccaag gccatttcta
     9961 ttatcagtga acgctaccaa ggaaaaaggc gaattcagat gttagggcta cgcgcttctg
    10021 cagaggtcgt tggagcttct ctcattacct tggccgtcgg tcagttgttg gcctttggtt
    10081 ggacagctat ctttctagcc tatagtgctg gatttttggt gctgcccctt tatctgctct
    10141 ttgtccctta tggaaaatca aagaaagaag tcaagaaaag agcgaaggaa gcaagtcgtt
    10201 taactcgaga aatgaaaggc ttgattttta ccttagctat cgaagcggca gttgtagttt
    10261 gtaccaatac agctattacc atccgtattc caagtttgat ggtggaaaga ggattggggg
    10321 atgcccagtt atctagtttt gttcttagta tcatgcagtt gatcgggatt gtggctgggg
    10381 tgagtttttc tttcttgatt tctatcttta aagagaaact gctcctctgg tctggtatta
    10441 cctttggctt ggggcaaatt gtgattgcct tgtcttcatc cttgtgggtg gtagtagcag
    10501 gaagtgttct ggctggattt gcctatagtg tagtcttgac gacggtcttt caacttgtct
    10561 ctgaacgaat tccagctaaa ctcctcaatc aagcaacttc atttgctgta ttaggctgta
    10621 gtttcggagc ctttacgacc ccatttgttc taggtgcaat tggcttacta actcacaatg
    10681 ggatgttggt ctttagtatc ttaggaggtt ggttgattgt aatctctatc tttgtcatgt
    10741 acctacttca gaagagagct taggattgat tcctaagttt ttcttttgat actcaatgaa
    10801 aatcaaagag caaactatag ttgattgagt ttggaatagt acgctgtagc tgctaaaata
    10861 tttctagaaa ttaatttgac tttcctaata gagttattca tatcttattt caatctacta
    10921 tagaaagcta gccgcaggtt gctcaaaata gtgttttgag gttgcagatg gaaactgacg
    10981 tggtttgaag agattttcga agagtatgag atttttatac ccatacaata tttgttttag
    11041 gtccttgttt acagactaat ttcttgataa aatagaggtt atgacgagat ataaagcaac
    11101 tatttcctat gatggttatg cctttgctgg ctttcagcgc cagtctcatg cgcgtagcgt
    11161 tcaggaagaa attgaaaaaa ccttgaccag attaaataaa gggcaagcca ttactgttca
    11221 cggtgctggt aggacagata gtggggttca tgccctggga caggtcattc attttgacct
    11281 gccttatcag attgatgagg agaaactccg ttttgccttg gatacccagt ctcctgaaga
    11341 tattgatgtg atttcgattg agcttgtggc agatgatttt cattgccgtt atgccaagca
    11401 tagcaagacc tatgagttta ttgtggatag aggacgtccc aaaaatccga tgcgccgtca
    11461 ctatgccacc cactttccct acccactcga tgtggaacga atgcagattg caatcaaaaa
    11521 gctagaggga acccatgatt ttaccggttt tacagcctct gggactagtg tagaggataa
    11581 ggttcgcacc atcacagaag ctagtttaat agtcgatgag acaggacaat ttttgacctt
    11641 taccttttcc ggaaatggtt tcttgtataa acagattcgc aatatggtgg ggactcttct
    11701 caaaatcggt aacaaccgca tgccagtaga gcagattgct ctcatcttgg agaagaagga
    11761 caggcaactt gcaggtccca ctgcagcacc aaatggtttg tatttaaagg agattcgtta
    11821 tgaagaataa tcgtatttta gcactttctg gaaatgatat ttttagtggt ggtggactgt
    11881 cagctgattt ggctacctat accttgaacg gcttgcatgg gtttgtagca gtgacttgtt
    11941 tgacagcctt gacagaaaaa ggatttgaag tctttccaac tgatgatacc atttttcaac
    12001 atgaattaga tagcttgcgt gatgtggaat ttgggggaat taagattggt cttctcccta
    12061 ctgtcagtgt ggctgagaag gccttggact ttatcaaaca acgcccagga gtacctgtgg
    12121 tgttggatcc tgtcttggtc tgcaaggaaa cgcatgatgt agctgtcagt gagctctgcc
    12181 aagagttgat tcgctttttc ccttatgtca gtgtgattac gcctaatctc ccagaagcag
    12241 aattattatc cggtcaggaa attaaaacct tggaagacat gaaaactgca gcgcagaaat
    12301 tgcatgattt aggagctcca gcagtcatta tcaagggagg caatcgtctt agtcaggaca
    12361 aggctgtgga tgtcttttat gatggacaga cctttactat cctagaaaat ccagttatcc
    12421 aaggccaaaa tgctggtgca ggttgtacct ttgcctctag cattgccagt cacttggtta
    12481 aaggtgataa acttttgcca gcagtagaaa gttctaaggt tttcgtttat cgtgctattg
    12541 cacaagcaga tcagtatgga gtaagacaat atgaagcaaa caaaaacaac taaaatcgcc
    12601 cttgtatccc tattaaccgc cctttctgtg gttctaggtt atttcttaaa aatcccaaca
    12661 cctacaggaa ttctaactct tttagatgct ggtgtcttct ttgcggcctt ttactttggt
    12721 agtcgtgaag gagcggtagt cggaggacta gcaagtttct tgcttgacct cttatcaggc
    12781 taccctcagt ggatgttttt tagcttggtc aaccatggct tgcagggatt tttcgcagga
    12841 tttaaaggaa aaagtcagtg gttaggcctt attttagcaa ctattgccat ggtagggggc
    12901 tacgccttgg gttctacttt gatgaatggc tgggcagcag ccctcccaga aattctacca
    12961 aattttatgc aaaatatggc agggatgatt gtaggattta ttcttagtca aagtatcaag
    13021 aagattaagt aatactcttc gaaaatctct tcaaaccacg tcggcttcca tctgcaacct
    13081 caaaacactg ttttgagcag cctgcggcta gctt
//
LOCUS       DAVBNO010000050        13075 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVBNO010000050 DAVBNO010000000
VERSION     DAVBNO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.050"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(278..1501)
                     /locus_tag="VQ846_001643"
     CDS             complement(278..1501)
                     /locus_tag="VQ846_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET3076186.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            1670..2992
                     /locus_tag="VQ846_001644"
     CDS             1670..2992
                     /locus_tag="VQ846_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET3076187.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGGIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTIENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            2994..4880
                     /locus_tag="VQ846_001645"
     CDS             2994..4880
                     /locus_tag="VQ846_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET3076188.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            5048..5392
                     /locus_tag="VQ846_001646"
     CDS             5048..5392
                     /locus_tag="VQ846_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET3076189.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            5402..6814
                     /gene="lacG"
                     /locus_tag="VQ846_001647"
     CDS             5402..6814
                     /gene="lacG"
                     /locus_tag="VQ846_001647"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET3076190.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVREFHQLGIEGKIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            6884..8563
                     /locus_tag="VQ846_001648"
     CDS             6884..8563
                     /locus_tag="VQ846_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET3076191.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGIIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QLISFVFLSLMLVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPCEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(8689..10128)
                     /locus_tag="VQ846_001649"
     CDS             complement(8689..10128)
                     /locus_tag="VQ846_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET3076192.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSVIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(10132..11481)
                     /gene="trkA"
                     /locus_tag="VQ846_001650"
     CDS             complement(10132..11481)
                     /gene="trkA"
                     /locus_tag="VQ846_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET3076193.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVNRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            11669..12517
                     /locus_tag="VQ846_001651"
     CDS             11669..12517
                     /locus_tag="VQ846_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET3076194.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            12532..>13075
                     /locus_tag="VQ846_001652"
     CDS             12532..>13075
                     /locus_tag="VQ846_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET3076195.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKK"
BASE COUNT         3999 a         2093 c         2791 g         4192 t
ORIGIN      
        1 gagaattgta aaaacaaaaa tggggatttc atataaagtt tattctagga gtatgtatct
       61 tgaataaaaa ttgttattta agttctgggg gttatgatga atgtttttat aataataaaa
      121 tgcagctaga agaaaagtta ttttaatata aatcactcaa catagagaga aggcggtttc
      181 ctatatggaa gtcgtcttgt tttcatattg tctaaatacg agatatttac tacaggttat
      241 agttgattta aacaggtcta tagatttcta atatatatta aggtgaaata atatcttgta
      301 atacattgct atgctctatg aaaaaagcaa cgatagctaa tgcacaagct cctatacttc
      361 cacgatgttt gttgtaaggt aaaatttcaa tttctatatt acgagtaaag ggaacgaagt
      421 ggagctggtc ttggatttta tccctgactt catgagtgaa aggttgataa ttaagcaatt
      481 gactgttgat atagatttta tcagcaggat tgatgaggag aagatttgca atagaagtgg
      541 ctaataaatt gactccttta tcaatttgtt gacgtaaagc ggagtcgcct aaattataag
      601 ccgttaaaag ggtgtctaaa tgaatgtctt tttcagtctt tacaaggctt tttagtacag
      661 ttagttggga atttttaaat aataattggg cgtgtttgat taaccaagca tccgaaatat
      721 atgtttgtaa acaacctttt tttccgcatt cacaatattg cccattcaat tcgacaatgg
      781 tgtgtccaat ttctccgata taaggatttt tagaggctcc tattttttct tttgtgaaaa
      841 aggaagtgta aatacctaat ccagcgtgta ggaaaataaa gttatcgggg gtattgtgtg
      901 gattaaaaag gtattgtcct atagccatac aatcgatatt attttttaca ataaaaggaa
      961 aattgaatct tttaattaca tttaataaat taaaagattc ccatatgggg ttgtttgtaa
     1021 tgatacttcc ggagtctttg ttataatgtc caggtattga taagccaaat ccaagtaatt
     1081 ttgtttccaa agggacgtac tctatagctg tttttatcat attggtaatg gtggaattga
     1141 tttctgaaat aggataatct tcattcaaaa tttctgtctc gaaaagagtt ttcccttgta
     1201 agtcacatag actgagaaag atacctagga cgtttatttc aatacctcca acaacatacg
     1261 gaatattatc tgtgatagaa atagtttttc tacgtcttcc agatccggaa aactcgctaa
     1321 attcatcacc agtttcgtat atgagggact gttcgcttag ctcttttatg atgttagtcg
     1381 ttgttgctgg agtaagtcct gtttcattaa caatatctgt acgggatgtt gctggatttc
     1441 gatatagaaa ctccagtata gtggctctat tttgtaaaaa ggctgattgt tttgtaatca
     1501 tttgtttcct cctttggttt attttatatt aaacataaga aaaaatcaat taaaattatt
     1561 attctataca aagaaaaaaa tattgtaaaa acaacaaaaa aatatattga caacgctttc
     1621 tgttagtggt atatttaatt tataaaaaat aaaaaaagga gttccggtga tgaacaatat
     1681 tttagcgttt ttagaaacaa aagtcgctcc gtttggtgaa aaagttggca accaacggca
     1741 tttgaaagct attcgtgaag gatttatgat ggcaatgcct ttgattttag tcggatcttt
     1801 atttcttatt ctaatcagtt ggcctcaaga ggattttaca aattggctga atagtgttgg
     1861 attgctaagt atcttgacaa ctatgaatca gtcaacagta gcgattatct ccttggtcgc
     1921 ttgtttcggt attgcctaca ggttgtcgga aggatatggt acagatggtc cgtcggcagg
     1981 gatcatagcc ttatccagtt ttgtattgat ggcacctcgt ttttcgagta tggtttatga
     2041 taaaaatggg gagcaggtca agcagttatt tggcggcgca ataccatttt ctagcctgaa
     2101 tgcatcttct ttgtttatgg cgattactat tggattggtt acagcagaga tttatcgtat
     2161 gtttatccag cgcggaatta cgataaaaat gccaagtggt gtcccagatg tggtaagtaa
     2221 atcattttca gctcttttac ctggctttac tacttttgtt ttgtgggctc tggtcttaaa
     2281 aggtcttgaa gcggcaggag ttgcaggagg tctcaacgga ctcctaggtg gaattgttgg
     2341 aacaccgctt aagttaattg caggaacgct tccaggtatg attctatgtg ttattgtaaa
     2401 ctcattcttt tggttctgtg gagttaatgg gggacaagtt ttaaatgctt ttgtagatcc
     2461 agtttggtta caatttacta tagaaaacca agaagctgtg gctgcaggac aaacactcca
     2521 acacattatt acattaccgt ttaaagattt atttgtattt attggtggcg gtggagcgac
     2581 tattggtctt gcgatttgtc tcttcctatt tagtaagagt cgtgcgaata aaacattagg
     2641 taagctagct attataccgt ctatttttaa tatcaataca gctattctat ttacgtttcc
     2701 aacagtttta aatccgatta tgctgattcc gtttattgct actcctacaa tcaatgcctt
     2761 gattacctat gtatcaatgg ctgtaggatt agtaccctat acaacaggtg taatccttcc
     2821 gtggacaatg ccaccgatta taggaggctt ccttgcaaca ggggctagtt ggcgaggagc
     2881 tctattacaa gttgttttga ttttggtttc tgtagcaatt tattatccat tcttcaaaat
     2941 tgcagataaa cgcaatcttg aaaaagaaaa agctactgtt ggagggaaat aagatggtta
     3001 tcagagtatt tgatcaacag aaaaatactt attctagctt tgccttagag gaattaagtt
     3061 actatatgaa tcgggtcttt aagactaaca tagagcttgt cgaggagaag gaagcggata
     3121 tttttgtagg attagtcaat aaagaggaca gaaaagacca tgttcttatc tcattagaca
     3181 agggtaaggg aagaattgag tctaatacaa ttgtaggttt acttattgga atttaccgaa
     3241 tgtttcatga atttggggtt gtgtatacta gaccagggcg cagacatgac tttgttccag
     3301 agttacgatt tgaagatttt ttagataaac agctatctat agatgaaaca gccagttact
     3361 atcatagggg agtatgtata gagggagcgg attcatttga aaatatacta gatttcattg
     3421 attggctacc taagattggg atgaacagtt ttttcatcca gtttgaaaat ccttactctt
     3481 ttttgaaacg ttggtatgaa catgaattta atccatatct aaataaagaa caattttcaa
     3541 atgaattagt acaagaattg agtgataggt tggataaaga attgcaaaaa agaggtctta
     3601 ttcatcatcg tgttggtcat ggatggacag gtgaagtttt aggttactct tcaaaatttg
     3661 gctgggaatc aggtcttagt atttcagagg agaagaaacc ctatgtcgct gaaataaacg
     3721 ggaaacgaga attgtttaat acggctccga ttttaaccag cctggatttt tcaaatccag
     3781 atgtagctga taagatggta gaaattatca aggattatgc caagaaaaga cctgatgtta
     3841 actacttaca tgtatggttg tcggatgctc gtaataatat ttgtgaatgc gaaaactgta
     3901 gacaagaatt ggtttcggat cagtatattc gtattctcaa tcaattggat agggctttaa
     3961 cgagtgaggg attagataca aagatttgtt ttctgcttta ccatgagttg ctatgggcgc
     4021 ctcagaaaga aaaattagat aatcctgaac gctttaccat gatgtttgca ccgattacaa
     4081 gaacatttga aatgagttat gcagatgtag attttgacaa ttccatacct acgcctaaac
     4141 cttatctgcg taataaaatt atacttccga attctcttga ggaaaattta tcttatcttt
     4201 ttgagtggca aaaaacattt aaaggagata gtttcgtata tgactatcct ttagggcgtg
     4261 ctcattatgg cgatttaggc tatatgaaaa ttagtcaaac catttacaga gatgtatctt
     4321 atctttctaa cctacatttg aacggttaca tttcgtgtca agaattacgt gccggattcc
     4381 ctcataattt tcctaattat gttatggggg aaatgctctg gaagaagaca agaagttatg
     4441 aagaattgat tgaagaatac ttttctgctt tgtatgggtc aaattggcag tctgttgttg
     4501 aatatttaga aaaattatcc agttattcct catgtgatta ttttaatgca attggcagtc
     4561 gtcaaaatga tgttttagcg aatcattatt atatagctta caatctagct gataattttt
     4621 tgccaattat tgaggaaaat atttctaagt tattaaatag tcaaaaggat gaatggaaac
     4681 agctcagtta tcatcgtgaa tatgttgtta agatggcgaa ggctttatat cttcaagcaa
     4741 ccggaaaaac aaggcaagct caagatgaat ggagaaatgt gttgaattat atccgtgggc
     4801 acgaactgct attccaatct aatttggatg tttatcgtgt aattgaagta gcaaaaaatt
     4861 acgctggttt ccacttataa atcataagta tagaaaatga accaaggtat tcagagaaga
     4921 ttgatcctaa atattatgaa atttaaggat ttttaagata tttagggtcg actttctatt
     4981 tatatcgtag cgaagtcatt ttaataatga tgtgtaaaag atggatcaag attgaggagg
     5041 aagaaagatg aaatcaaaag aagaaataaa tatgcttggt tttacaattg tcgcttacgc
     5101 aggagatgca aggtcagatt tgatggatgc tttggcgttt gcgagagatg gatattttga
     5161 acaggcaaga gaattggttg agtctgcaaa cgactcaata gtgtctgccc atcgagaaca
     5221 gactaattta ttagcggagg aggcatctgg agataatttt gaagtgagtt ttatcatgat
     5281 tcatggtcaa gataccttga tgacaacgat gctattgtat gatcaggtca agttttttat
     5341 tgatgaatat gaacgaattc gaaagattga agaacatatt ggtttgcaat gaggattagt
     5401 catggaaaat ttacaggtta aagccttacc gaaggagttt ttattaggag ctgctaccgc
     5461 tgcttatcaa gtagagggtg caactagggt agatggcaaa ggaacaaata tgtgggatgt
     5521 ttatttgcaa gaaaacagtc cgtttttacc agatccggct agtgattttt attatcgtta
     5581 cgaagaggat atagctttgg cggcagaaca tggtttgcag gctttgcgtt tatctatttc
     5641 ttgggttcgt atatttcctg atatagatgg ggagcctaat atattagctg ttcgttatta
     5701 ccatagagtc tttcagtctt gcttaaaaca taatgtgatt ccgtttgttt ctttacatca
     5761 ttttgattcg cctcagaaaa tgttagaaac aggggattgg ttgaatagag agaatattga
     5821 tcgtttcata cgatatgctc gcttttgttt ccaagaattt acagaagtca agcattggtt
     5881 tacaatcaat gaactgatgt ctcttgctgc aggtcaatat ataggaggtc agtttcctcc
     5941 aaatcatcat tttcaattat ctgaagcaat tcaagcgaat cataatatgt tgttggcgca
     6001 tgctcttgca gtccgcgaat ttcatcaatt agggattgag ggaaagatag gttgtattca
     6061 tgctttaaag ccaggctatc ctattgatgg gcaaaaagaa aatattttgg cagctaaacg
     6121 gtatgatgtt tataataata aatttctatt agatggaact tttttgggct actacagtga
     6181 ggacacgctt tttcacttga atcaaatatt ggaagctaat aattctagct ttattattga
     6241 agatggtgat ttagaaatta tgaagagagc tgcagctctt aatacgatgt ttgggatgaa
     6301 ttattatcgt tcagaattta ttcgtgaata caaaggtgaa aatagacaag aatttaattc
     6361 aacaggaata aaaggacagt cttcttttaa attaaatgct ctaggtgaat ttgtaaaaaa
     6421 acctggtatt ccgacaacag attgggattg gaatatttat cctcaagggt tatttgatat
     6481 gttgcttcgt atcaaagaag aatatcctca acatccggtc atttatttaa ctgaaaatgg
     6541 tacagccctt aaagaagtta agccagaggg cgagaatgat attattgatg acagtaagag
     6601 aatccgttat attgagcaac atttacacaa agttttagag gctcgagata gaggagtcaa
     6661 tattcaaggc tattttatat ggtctttgca agatcaattt tcttgggcga atggctacaa
     6721 taagcgatat ggtcttttct ttgttgatta tgaaacacag aagagatata ttaagaaaag
     6781 tgctctttgg gtaaaagggc taaaacggaa ttaaggttag cgatttgact gatgtttaat
     6841 atattttaac tatgagctta aattttttac aggaggagtt tttatggata agttagtcgc
     6901 tgccattgaa aagcaacaag ggaaatttga aaaaatttct actaataatt atatgatggc
     6961 tattaaagat ggatttattg ctactatgcc tttaattatg ttttcaagct ttttgatgat
     7021 tattattatg attcctaaaa atttcggagt agagttaccg agtccagcta ttgcctggat
     7081 gaggaaagtg tatatgctaa ccatgggagt tttgggtatt attgtttcag ggacagttgc
     7141 aaagtcattg gctggaaatg ttaatagaaa aatgcctcct ggaaaggtaa taaatgatac
     7201 ttctgcaatg ttggcggcca tatgtggcta tcttgtatta actgtaacgc ttgtagttga
     7261 tgagaaaacg ggatctacaa gtttgtcgac aaactattta ggatctcaag gattgataac
     7321 ttcgtttgtc agtgccttta ttactgtaaa tgtttaccga ttctgtatta agcgagacat
     7381 tactattcat ttacctaagg aagttcctgg ggctatatca caagctttta gagatatttt
     7441 tcctttttct tttgttttac ttattagtgg tttgttagat attgtatctc gttttagttt
     7501 agatgttcct tttgcccaag tatttcaaca actattgact cctattttta agggggcaga
     7561 atcatatcct gctatgatgt tgatttggtt tatgtgtgct ttgctttggt ttgttggaat
     7621 tcatggacca tctattgtct tacctgctgt tacagcttta caactgagca atatggaaga
     7681 gaatgctcaa cttcttgcaa atgggcagtt cccttatcat tctttaacac ctaatttcgg
     7741 gaattatatc gctgctattg gaggaacggg ggctaccttt gttgtaccat ttattttgat
     7801 tttctttatg cggtctaaac aattaaaatc ggtaggtaaa gctacaatta ctcctgtttt
     7861 atttgcggta aatgaacctc ttctatttgg tatgcctgtt attttgaatc cctatctttt
     7921 tgtacctttt ttgatgactc caccagtgaa tgtatttcta ggaaaggtat ttattgattt
     7981 ctttggaatg aatggatttt atatccagtt accttgggcc tttcctggtc ccttgggatt
     8041 gttaattgga acgaattttc aacttatctc ctttgtattt ttatctttga tgttagttgt
     8101 cgacatattg atttatttgc cattctgtag agcgtatgat agacagttac tggtgaaaga
     8161 agatattgca agctcaaatg atattatttt agaggaggat acaagtgaaa taatgccttg
     8221 tgagatagat gtaataaaaa gtaaggagtt gaaagtactg gttctttgtg cagggtctgg
     8281 aacaagtgcg caattagcca atgcaattaa cgagggcgct caattagcag aagttagagt
     8341 gattgcgaat tcaggagcgt acggagctca ttatgatatt atgggtgttt atgatttaat
     8401 tattctggcc ccacaagttc ggagttatta tagagagatg aaggtggatg cagaaagatt
     8461 aggtattcag atagttgcta ccagaggaat ggaatatatt catttaacaa agagtccaag
     8521 taaagcctta caatttgtat tggagcatta ccaagctgtg tagtaagttt ttccatcttt
     8581 tatttgagta aagattttgc ttacagatag gcttggattt aaaaacgttc cccctttttt
     8641 aatataagaa tccctctttc acaattgtaa aaagagggat tttgtatttt atctcttaga
     8701 ccaagttctc ttcataaaga gaaggaggat tgggtaaatc tccaagcgcc ctgcaatcat
     8761 tgcaaaggat aggagaattt ttgagatggg actaaagatt gagaaactag aagtggttcc
     8821 tagaataggc ccgatattat tgaaacagct aaagacagcg ctggtcacga ccagaaaatc
     8881 attgctatct aggctgacaa taaagataag cgctagcaaa atcatagcat agatgacaaa
     8941 gtacttgaga atcttatgct gggtatcttt gtcaatcacc gttttattaa catggagggt
     9001 caaaacacgg tggggcgata ggattgacaa aatttggttt ttggcaattt ttgaaaggat
     9061 gaggcctcga ataatcttga gtccacctgc agttgatcca gcagagccac cgattgccat
     9121 gaggaaaagg aggataaact gggagaagag gggccagttg gtaatatctc catatccaaa
     9181 accagttgtt gtaatgatgt tggaaacctg gaagaaggtc atttcaaagc tctttgaaaa
     9241 ccctgggtag aggtagaggg tgttgaggct aatcaagcct gtagaaacca gtacaatgac
     9301 caagtaagcc cgaagttctt cgtcaccaaa gaaggccttg atgcgacgga gcatgaggta
     9361 gtagtagagg ttgaaattta ccccaaaaac cagaactccg atactgacca gataggtaat
     9421 cagtgagctg ccatagtggg caattccgtc gttatagacg gtaaagcctc cagttcccgc
     9481 tgtccccata gcaatgacaa aactatcgta gagaggcata ccagctagat aatagatgat
     9541 gacaaagagg gagaagagag ctagataaag gagatagaga atctgggcag tgttttttag
     9601 tttggataca accttgccaa aaacaggacc tggaacctca gccttcatca cctctaggtg
     9661 gctatttttg gcattgtcca taatagcaag tgcaaaaaca agcactccca tccctccaat
     9721 caagtgggta aaacttcgcc agaagaggag ggaacggctg agaaccgaaa tgtcgttcaa
     9781 aatacttgct ccagtagttg taaacccaga actaatttca aaaaaggcat caataacgct
     9841 agggatttgc ccagaaaaga caaaggggag accaccaaag aaagaccaaa ggatccaaca
     9901 gagggcaacg atcaagactc cctccttggc ataaatccgt tgattttttg gcttctgtaa
     9961 actccctgaa ccgcctaaca atacgagaat ccctatggtc gaaaagaggg ctgtaaagac
    10021 ttggctcgat tcacggtaat agacagcgat agccacagga accaagagga gaacagcttc
    10081 aatcaaaagt aactttgaaa ggaggtaacg aatcatactt ttattcattt cttacctcgc
    10141 aatcaagtca taaatcttgg tgatgtttgg caacaaggtt gttactagga gcttgtctcc
    10201 aacttccaac atatcctccc cagttgggaa aatagtcttg ccctttcgaa taatggctgc
    10261 aataagaacc ccttttttca atttcagttg agaaagaggt ttggcagtca ttttattggc
    10321 ttccttgata tggaattgca gggtttcaat ttggccattg gctagatggt gcatggcttg
    10381 aaggtctgaa tactgggcat taactcgacc acgaataaag tgcataatcg tatctacagc
    10441 gatgctttta ggtgtgatga tacttgaaaa atcaggcgca ttgataatct cgaggagact
    10501 ggtacgattg accttagtaa tatttttctg tacacctacc ctgtcaagga acatagatgt
    10561 aatcagattt tcctcatcga cacctgttag agtcgcaacg gcatcatagt gttgagcact
    10621 ttcttccagc aggatatctt ttgcggttcc atctccttga acgatgtaga gatttgggaa
    10681 tttctcgcta aagaagctgg cgatttcagg attgatttca atgacttttg tatcgatacg
    10741 actatctttg agaataccaa gtagataata ggcaattcta cctgccccaa cgatgagaag
    10801 gctcttcacg gcgcgtgatt taaaataatt atggaagagt atcatatcga cacggttacc
    10861 agtgacaaag attctatctt tatcctgtac agtcatgtca ccgcttggaa tgataatttg
    10921 atgatccctc tctatcgcac agacaatgac attaccaaat tttttacgaa aatcagaaat
    10981 gggcatttgg caaagaccgc tggtggactt gacgacaaat tccatgaggc taacgcgtcc
    11041 accagcaaag cgttcgacag acagggcgtt ggggaagtca atgatattcg cgatagcgcg
    11101 ggcagccaag agctcaggat taacgataag agaaaaaccg agaatattct tttccttgaa
    11161 ataagagtta gaatattcag ggttccgcac ccgaacgata gtttctttag ctcccatttt
    11221 cttggctaga actgctgcaa tcatgttgac ttcatcgtgc tcagtcaggg cgataaagat
    11281 atcacaatct tggacgctgg cttgctcaag aatggcaaaa tcggccccgt taccaaggat
    11341 accaatgata tcaaagcgat tgacaatatg attgagaaca gcttcgtctt gctcaatcag
    11401 caaaacatca tgcttttctg caaccaagga gcgacagagg gcaaaaccaa cttttccccc
    11461 tccgacaagg ataattttca taataaaacc tactttttca tgatgtaact atcataccct
    11521 ttttcaagaa aaaatgcacc tactagctaa taacaagagc ttttagtgaa aattcgctat
    11581 aaggtaaaac tataccctaa ccaattgaaa tagctattag cgactttctc tgaaatatgg
    11641 tatgataaag gatatacaag gagataaaat gaataataat ttactggtat tacaatcaga
    11701 ctttggtctg gttgatggtg cggtatcggc tatgattgga gtggctttag aagagtctcc
    11761 aaccttaaaa atccatcact tgacgcacga tatcacgcct tataatattt ttgaggggag
    11821 ctatcgtctc tttcagacgg tggattactg gcctgaggga acgacgtttg tatcggttgt
    11881 cgatccaggt gtcggttcga aacgtaagag tgtagttgcc aagactgcaa aaaatcaata
    11941 cattgtcacg ccagataatg ggacgctttc ctttatcaag aaacacgttg gcattgtagc
    12001 cattcgtgag atttctgagg tggccaatag gcgtcaaaac acagagcatt cttatacctt
    12061 ccacggtcgt gatgtctatg cctatactgg tgctaaactg gccagtggtc acattacttt
    12121 tgaggaagta gggccagagc tcagtgtgga acagattgta gagcttccag tcgtagcgac
    12181 catcatagaa gatcatctgg tgaagggagc cattgatatt ctggatgtgc gtttcggttc
    12241 gctttggacc tctatcacac gggaagaatt ttacaagctg gaaccagaat ttggtgatcg
    12301 ttttgaagtg accatctatc atgctgatat gctggtctat caaaatcagg ttgtctatgg
    12361 caaatcattt gcagatgtga gaattgggca acccatcctt tacatcaact ctctctatcg
    12421 tttaggtttg gctatcaacc aaggttcctt tgccaaggcc tataatgtag gtgtcggttc
    12481 atcttggacc attgaaataa agaaaataga aggataaaat aggagaatag tatggaaatc
    12541 aaatttacaa ttaaacaagt tgttgctgtc ggaattggcg ctgccctctt tgtcgtcatc
    12601 gggatgatca acattccaac ccctgttcca aatacaagca tccagcttca gtatgcggta
    12661 caagcgctac tttctattat ttttggaccg attatcggtt tgcttgtcgg gttgattggt
    12721 catgcaatca aggactctct tgctggttat ggtctatggt ggacttggat tatcgctagc
    12781 ggactttttg gtctagttgt gggacttttt agaaagtacg ttcgagtgat caatggtgtt
    12841 tttgactgga aagatattct tatttttaac ctcattcaac tacttgcaaa tgcccttgtt
    12901 tggggtgtct tggcaccact tggagatgtt gtgatttatc aagaagcggc agaaaaagta
    12961 tttgcacaag ggattgttgc gggaattgcc aatggtgtat ctgtagctat tgcaggaact
    13021 cttctcttac ttgcctatgc aggaacccaa actcgtgcag gaagtttgaa aaagg
//
LOCUS       DAVBNO010000051        12973 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVBNO010000051 DAVBNO010000000
VERSION     DAVBNO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12973
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.051"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            87..3437
                     /locus_tag="VQ846_001653"
     CDS             87..3437
                     /locus_tag="VQ846_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET3076196.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFVFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            3681..3782
                     /locus_tag="VQ846_001654"
                     /pseudo
     CDS             3681..3782
                     /locus_tag="VQ846_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4023..4493)
                     /locus_tag="VQ846_001655"
     CDS             complement(4023..4493)
                     /locus_tag="VQ846_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET3076197.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4510..6783)
                     /locus_tag="VQ846_001656"
     CDS             complement(4510..6783)
                     /locus_tag="VQ846_001656"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET3076198.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGQAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCKTFSLGQEEQ
                     AIQNQYPDKDFERYSKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            7129..10230
                     /locus_tag="VQ846_001657"
     CDS             7129..10230
                     /locus_tag="VQ846_001657"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET3076199.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDEQEVNLRFLSLSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNYSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            10313..11320
                     /gene="pfkA"
                     /locus_tag="VQ846_001658"
     CDS             10313..11320
                     /gene="pfkA"
                     /locus_tag="VQ846_001658"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET3076200.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            11379..12884
                     /gene="pyk"
                     /locus_tag="VQ846_001659"
     CDS             11379..12884
                     /gene="pyk"
                     /locus_tag="VQ846_001659"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET3076201.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATNSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
BASE COUNT         4021 a         2204 c         2851 g         3897 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttgcatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtagaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctc ttgagaaaca tatcggctat cagccaattt
      961 gctttattac aaatggcttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cacttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggctgggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcct
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcgc atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt tcactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tatttaattc ctttgttttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgatca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgttgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagct ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctctggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggctga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagccgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaattagct gcaggctgct caaaacactg ttttgaggtt gtagataaga
     3781 ctgacgaagt cagctcaaaa catggttttg aggttgtaga tgaaactgac gaagtcagta
     3841 accataccta cggcaaggcg acgttgacgc ggattgaaga gattttcgaa gagtataaat
     3901 cttcctagga taaagcaaaa cgcatagtat caagtgttga aaacccttga tactatgcgt
     3961 tttctgatgt taaagacttt ctaccagttt ttttaagcat aattgttagt tgtagtcatt
     4021 tattattctt caaagaaaaa tggtggggcg aattttttca gttcttcaaa gcacttttga
     4081 gcagtatctg catcttcaca gatgataaga cagacatcat taccacaaag ggtagcgata
     4141 gcgtcaggga agctcaaagt atcaatgata gaaccaaagg attgagccag tccaggaagg
     4201 gtttttagta ggacttggtg ttgaactggg cgcatccaga caagggcgtc ttccatgtag
     4261 agttcgagac gtttttccca ttttgagatg gaaccattgt taagaacata ataagcgcta
     4321 tcttcttcgc ggacttttga taggttcata tttttgatgt cgcgtgagag ggttgcctgg
     4381 gttacttgaa tgtcgttctt agcaagaagg gcttgcaact cagcctgtgt atgaatcttg
     4441 ttttttgtga taagagcgcg tataagttgg tggcggtgtt ctgatttatt cataataatg
     4501 taactccttt tagcaaggta aggtaagcat ggactgagcg aggtcgacag tcaagtggta
     4561 gtctgtattg tcacggatgg tgatttcaaa gtcagtagta tagaggacta aacggagagt
     4621 gtctccttct tttagcttgt aaatagttgg ctgcagttca aattgaacgt ccatccattc
     4681 atctgcagta atatcctcta ctaacagtaa atcatttcta ttttgtaaat taaggtaacc
     4741 ttttgtcacg actcgttgtg cctctggtct aaatggcaat tcacagagat tttccaacat
     4801 gtgatagcga ccgttatcaa tggttctagc acttaaaata gctggataag gttgtaggta
     4861 tttcttttgc ccaaattcta gcagttgggc agataagagc cccttgtttg tactggattt
     4921 gatacgaaga ttgagctgag cgcgaccgtt taggtggaga tctttagtca caggaaggtt
     4981 aatagtaatc tgattagctt tcccttgata gagctctgta ttgaaggttt ggtatgtctt
     5041 actatagcgc tcaaaatcct tatctgggta ctggttttga atagcttgct cttcttgacc
     5101 aagtgagaag gttttacagt tttcttgccc accgaagtta tcaagtgata accaagtctg
     5161 tggagctgta ttgtcctgcc agataacagt aggaagttga aagtctgttt cctgtcctag
     5221 taatttcttg gttaataagg catttatgga ctcacggaag tcaattgatt gccaattgtt
     5281 catgtaaaca tgggcaccat tatggaaaaa gagatgcttg tgtatatgag taggaagagc
     5341 atggaacatc tggtaaacat gaagtggttt gacattccaa tcctgagaac catgagtaaa
     5401 gacaacctct gcctttactt tatgggcatt gagcagataa ttgcggtcat gccaaaactg
     5461 attgtagtcc ccagtttttc ggtctagctg agctttcact ttttctaagt cagcttggtg
     5521 agcttcattg ccacggatat agtcgccagc taagagatta cgagaatagg ttaactcagc
     5581 aagggagtca aagtcctcac ctggataacc acctgggcta gtcaccagac cgttttcacg
     5641 gtagtagttg taccatgatg aaattcctgc ctcggcaatg ataacttcta aaccatcgac
     5701 tcctgtagtc gcaagaccat tggacatggt acctagatag gaaagtcccg ttgtagcaac
     5761 ttttccgttt gaccaatcag ccttgacttg acgctggcgc gtgtgatcag taaaggcacg
     5821 gcaacggccg ttaagccaat cgatgacatt tttataagcc tcgatttgct ggtagtctcc
     5881 attagtcatg aaacctgtcg agtctttggt accaacacct gagacataga gattggcaaa
     5941 gcctctcgga aggaagtagt cgtttagtgt atagctagag ttgatgtgag ttagcttttc
     6001 ctcagcctct gctataagct cagcttgacc ttggggttgg acgagattta gttgaggttt
     6061 ctctagctca atcttgtgag gaagcttaac ctcaagctcg ccctccatct tgtagagagc
     6121 cttgtcacta gccttgtcat tggttccctg atgataaggg ctggctgtca tgatggcagg
     6181 gatttttcca tcaaaacgag ggcgaataat gctaaccttt actaggtctg atagcccttt
     6241 ttggtcagta tcgacacgag actcaacgta aacgacttca cgaatgacat cctggttaga
     6301 aaaagtagcc aaactcttgc cgttaaagta gtggtagtca ttatcctccg gaataagacc
     6361 atcactaaca agttggtcga taagagtatt tccttttttg gtgcgagtat tgagtaactg
     6421 atagagattt tcaatcaagt caccatatat aatgggaaat ccagtttctt tacgaaaaac
     6481 gtcactatct tcgaagtcaa ccaaataaga aaagcctaaa agttgaaaag caacagtata
     6541 aaaaatatct gctgtcagtt catcttctga ttgaaaaaat gtcagcaggt ctgttttttt
     6601 atcagctgct aggatagaaa gtgggtagtt ggtgtcttga taagtgaaaa agaaacgacg
     6661 taaaaaggtt tcaagtgagt ctttgtgatt ggctgtattt tgtaaatcaa agccacattt
     6721 ttttagctca gataagacat tttcttttgg aaaattgata taactatatt gattaaaacg
     6781 catagaacct ccatatagaa tgacagttaa ggttattata tcaaaaaaaa agcagaaagg
     6841 gaattgttaa cttcaaaagg aaataatcca ataaaaatga ataaagtact aaattcaata
     6901 tagagaacag agtaacaata agaataatag atagggtata aaagttctag gagatttata
     6961 ttatatgctt tctattttta tatacaatat agtataaata taaaaatgat gacaaaaata
     7021 caaatgaata gaaaataaat tagtaagctg atgaaatttt tctcaagaga agccatttat
     7081 aggtgaaaat ggtataatat agtgagaagg atagaggaga agtgtaaatt gatcgcacaa
     7141 ctagatacaa aaacagtcta tagttttatg gaaagcgtca tttcgatcga aaagtatgtg
     7201 agagcagcta aagaatacgg ctacactcat ttggctatga tggatattga caatctttat
     7261 ggcgctttcg actttctaga gattacaaaa aaatacggca ttcatccttt gctagggctt
     7321 gaaatgacag tgtttgtaga tgaacaggaa gtgaatttgc gctttttatc tctatctagt
     7381 gtgggctatc agcagttgat gaagctttcg acagccaaga tgcaggggga gaaaacttgg
     7441 tcagtcctgt cccagtacct ggaggatatc gcggtcattg tgccttattt tgatagagtt
     7501 gagtcgttag aactaggctg tgattactat ataggggttt atccagaaac actagcaagc
     7561 gaatttcatc atcctatctt acctctttat cgggtcaacg cttttgaaag cagggataga
     7621 gaagttcttc aagttttaac agcgattaaa gaaaatctac cgctcagaga agttcccttg
     7681 cgttcgagac aagatgtctt tatatcagca agttctttag agaaactatt ccaagagtgt
     7741 tttccgcaag ctttggacaa tttagaaaag cttatttcag gcatttctta cgacttggat
     7801 actagtctga aactgcctcg ttttaatcca gctagaccag cagtagagga gttgagagag
     7861 cgtgctgaac tggggcttgt tcagaagggg ttgactagta aagaatatca agatagacta
     7921 gaccaagaat tgtctgttat tcatgatatg ggctttgatg attatttctt ggttgtttgg
     7981 gatttgttgc gttttggacg atcgaatggc tattatatgg gaatgggaag gggttctgca
     8041 gtaggcagtt tggtttctta tgccttagac atcacgggga ttgacccagt agagaaaaat
     8101 ctgatttttg aacgctttct taatcgtgaa cgctatacca tgcctgatat tgatattgat
     8161 atcccagata tttatcgtcc agattttatc agatatgttg gtaataaata tggtagtaaa
     8221 catgcggcac aaatcgttac tttttcaacc tttggagcca agcaagctct tcgagatgtc
     8281 ttgaaacgct ttggtgtgcc agagtatgaa ttatctgcaa ttactaagaa aatcagtttt
     8341 cgtgacaatc ttaagtcggc ctatgagggc aatctccagt ttcgtcagca aatcaatagt
     8401 aagttagaat accaaaaagc ttttgagatt gcttgcaaga tagagggcta tccaaggcaa
     8461 acctctgtcc atgcggctgg tgttgtaatt agtgaccaag atttaaccaa ctacattcct
     8521 ctaaagtatg gtgatgaaat tccactgact cagtatgatg ctcatggagt tgaggctagc
     8581 ggacttttga agatggactt tctgggacta cgaaatttga cctttgtcca gaagatgcaa
     8641 gagttgcttg ctgaaacaga aggtattcac cttaaaattg aagaaataga tttggaagac
     8701 aaagaaacgt tagatttatt tgcctctgga aatacaaaag gtatctttca atttgagcaa
     8761 cctggtgcca ttcgcttgct caaacgtgtt caaccagtct gttttgaaga tgtcgtagca
     8821 actacttctc taaatcgacc aggtgcaagt gactacatca ataattttgt ggcaaggaag
     8881 catgggcagg aagaagtgac tgttctagat tcagctctgg aggatatttt ggctccaact
     8941 tatggcatta tgctctatca ggagcaggtt atgcaggttg ctcagcgatt tgctggattt
     9001 agtcttggga aggccgatat tttgcgtcga gctatgggta aaaaggatgc ctctgctatg
     9061 catgagatga gggcttcctt tattcaaggc tccatagaag caggccatac tgcggaaaaa
     9121 gcagagcagg tttttgatgt tatggagaag tttgcaggtt atggatttaa tagatctcat
     9181 gcctacgcct actcagcctt ggccttccag ttggcttatt tcaaaacgca ttatccagcc
     9241 attttttatc aggtcatgtt aaattattcc aacagtgatt acttaataga tgcacttgaa
     9301 gcaggttttg aagtagcctc tctatccatc aatactattc cctatcacga taaaattgcc
     9361 aacaagtcta tctatatagg tttgaaatcg attaaggggc tcagcaagga cttggcgctt
     9421 tggattattg aacatagacc ttattctaac attgaagatt ttatagctaa attacctgag
     9481 aattatctga aacttcctct gctagaacct ttggtaaaag ttggtctttt cgattcattt
     9541 gaaaaaaatc gtcaaaaagt atttaataac ttagctaatc tatttgaatt tgtgaaagag
     9601 ttgggaagtt tgtttggaga tgctatttat agttggcagg aatcggaaga ttggacggaa
     9661 caagaaaaat tttatatgga acaagagctt ttagggatag gtgtcagcaa acatccacta
     9721 caagctattg caagtaaggc tatttacccg attaccccaa tcggaaattt gtcagaaaat
     9781 agctatgcta ttattttggt tgaagttcag aaaataaaag tgattcgtac caaaaagggt
     9841 gaaaatatgg ccttcttaca ggcagatgat agtaagaaaa aattggatgt cactctcttt
     9901 tcagacttat atcgtcaggt tggacaggaa ataaaagagg gagccttcta ctatgtaaaa
     9961 ggaaaaatac aatcacgtga tggccgtttg caaatgattg cacaagaaat aagagaagca
    10021 gttgctgaac gcttttggat acaggtgaaa aatcatgaat cggatcaaga aatttcacgt
    10081 attttagaac aatttaaagg cccaatccca gtcatcatcc ggtatgaaga ggaacagaaa
    10141 accatcgttt ctccccatca ttttgtagct aaatccaatg aattagagga gaaattgaat
    10201 gaaatcgtta tgaaaacgat ttatcgctaa aaatacggaa aatagaagaa ttttaaaatc
    10261 aaatgtgata taatcaagaa gaatgttaaa agaaaaagga gcataaacca atatgaaacg
    10321 tattgctgtt ttgactagtg gcggagacgc acctggtatg aacgctgcta tccgtgcagt
    10381 tgttcgtcaa gcaatttcag aaggaatgga agtgtttggt atctatgacg gatatgctgg
    10441 tatggttgcc ggtgaaattc atcccctaga tgcagcttca gtaggagaca tcatttctcg
    10501 tggtggtact tttcttcact cagctcgtta cccagagttc gctcaacttg aagggcaact
    10561 taaagggatt gagcaattga aaaaacacgg aattgaaggt gtagttgtta tcggtggtga
    10621 cggatcttac cacggcgcta tgcgtttgac tgaacatggc ttcccagcta ttggtcttcc
    10681 aggtacaatc gataacgata tcgttggtac tgactttaca atcggttttg acacagcggt
    10741 tactactgcc atggacgcta tcgataagat tcgtgataca tcatcaagtc accgtcgtac
    10801 ttttgtaatc gaagttatgg gacgtaacgc tggtgatatc gctctttggg ctggtattgc
    10861 aactggtgct gatgaaatca tcatccctga agcaggcttc aagatggaag atatcgtagc
    10921 aagcatcaaa gctggttatg aatgtggtaa aaaacacaat atcatcgtct tagctgaagg
    10981 tgtgatgtca gcggctgaat ttggtcaaaa acttaaagaa gctggagata caagcgacct
    11041 tcgtgtaaca gaacttggac atattcaacg tggtggttct ccaactgcgc gtgaccgtgt
    11101 tttagcgtca cgtatgggtg cacatgctgt taaacttctt aaagaaggta tcggtggtgt
    11161 tgcggttggt attcgtaacg aaaaaatggt tgaaaatcca attcttggta ctgcagaaga
    11221 aggggcattg tttagcctta ctgcagaagg taagattgtg gttaacaacc cgcacaaagc
    11281 cgatattgag ctatctagct tgaataagag cttgtcataa tttacaattt agttaataag
    11341 accaaaaagg tcatatatat aaaggagtca caaaaatcat gaacaaacgt gtaaaaatcg
    11401 ttgcaacttt gggtcctgcg gtagaaatcc gtggtggtaa aaaattcggt gaggacggat
    11461 actggggtga aaaacttgat gttgaagctt cagctaaaaa cattgctaaa ttgattgaag
    11521 ctggtgctaa cacattccga ttcaacttct cacacggcga ccaccaagaa caaggtgagc
    11581 gtatggcaac tgttaaactt gcggaaaaaa ttgcaggtaa aaaagttggt ttccttcttg
    11641 atacaaaagg acctgaaatc cgtacagaat tgttcgaagg tgaagctaaa gaatattcat
    11701 acaaaactgg tgaaaaaatt cgtgttgcaa ctaaacaagg aatcaaatca actcgtgaag
    11761 tgattgcgtt gaacgttgct ggtgctcttg atatctatga tgatgttgaa gttggtcgtc
    11821 aagttttggt tgacgatggt aaacttggtc ttcgtgtggt tgctaaagat gatgcaactc
    11881 gtgaatttga agttgaagtt gaaaacgatg gtatcatcgc taaacaaaaa ggtgtgaaca
    11941 tccctaacac taaaattcct ttcccagctc ttgctgaacg cgataacgac gatatccgtt
    12001 tcggtcttga acaaggtatc aacttcatcg caatttcatt cgtacgtact gcaaaagatg
    12061 tgaacgaagt tcgtgcaatc tgtgaagaaa ctggaaacgg acatgttcaa ttgttcgcta
    12121 aaatcgaaaa ccaacaaggt atcgataact tagacgaaat catcgaagca gctgatggta
    12181 ttatgattgc tcgtggtgat atgggtatcg aagtaccgtt cgaaatggtt ccagtttatc
    12241 aaaaaatgat tatcaagaaa gtcaatgctg caggtaaagt tgttatcact gcaacaaaca
    12301 tgcttgaaac aatgactgaa aaaccacgtg caactcgttc agaagtatca gacgtattca
    12361 acgctgttat cgacggaact gacgctacaa tgctgtcagg cgagtctgca aacggtaaat
    12421 acccactcga gtcagtaact acaatggcta caatcgacaa gaacgctcaa gctcttctta
    12481 atgaatacgg acgtcttgat tcagattcat ttgagcgtaa ctctaagaca gaagtaatgg
    12541 cttctgctgt taaagatgct actaactcaa tggatatcaa attggttgta actcttacta
    12601 agacaggtca tactgcacgt ttgatttcta aataccgtcc aaatgctgac atcttagcat
    12661 tgacatttga cgaattgaca gaacgtggct tgatgttgaa ctggggtgtt atcccaatgt
    12721 tgacagatgc tccatcttca actgacgata tgttcgaaat cgctgaacgt aaagcggtag
    12781 aagcaggtct cgttgagtca ggcgatgata tcgttatcgt tgctggtgtg ccagtaggag
    12841 aagctgttcg cacaaacaca atgcgtatcc gcacagtacg ttaagaaaaa tataaaaacc
    12901 tatcatatcc agctttctag cctgtatgat gggctctttt tttgtccagg gggcaaaact
    12961 ttcataatgg att
//
LOCUS       DAVBNO010000052        12573 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVBNO010000052 DAVBNO010000000
VERSION     DAVBNO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.052"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(251..433)
                     /locus_tag="VQ846_001660"
     CDS             complement(251..433)
                     /locus_tag="VQ846_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076202.1"
                     /translation="MAVIFKNVLIEEKTEEEKQHILSILNGEYDLSDDVSCRLRRLNE
                     ESKRLEVFVKETNSLV"
     gene            complement(649..1881)
                     /locus_tag="VQ846_001661"
     CDS             complement(649..1881)
                     /locus_tag="VQ846_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET3076203.1"
                     /translation="MKIAVAGTGYVGLSIAILLAQHHQVVAVDIIPEKVELINKKKSP
                     IQDDYIEKYLAEKELNLVATLNAEEAYENADFIVIAAPTNYDPKKNFFDTSSVEAVIE
                     AVMKYNPDAIIIIKSTVPVGYTKSVRRKFLTDNIMFSPEFLRESKALYDNLYPSRIII
                     GTDKDDKDLVKSAEIFVKMLQEGAVKENIETLYMDFTEAEAVKLFANTYLALRVSYFN
                     ELDTYSEMKGLDTQAIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYSDVPQ
                     EMITAIVQSNRTRKDYIADRILDLAGSYVGNDFYKPDKEKEIVVGIYRLTMKSNSDNF
                     RQSSIQGIMKRLKAKGVTIIIYEPTLESGTTFYGSQVVNDFEIFKKSSQVIVANRFDE
                     TLEDVRGKVYTRDIFKRD"
     gene            complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ846_001662"
     CDS             complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQ846_001662"
                     /EC_number="5.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate 4-epimerase Gla"
                     /protein_id="HET3076204.1"
                     /translation="MRGKTILITGVAGFIGSNLVRKLLELEGTMTIIGLDNLNDYYDI
                     ALKDYRLLQLNKLIQDYPEKNWIFIKGDIADKMLINKIFKSYHPQIVVNLAAQAGVRY
                     SITNPDVYIESNIVGFHNVLEACRHSYEIYDGGVEHLVYASSSSVYGGNKKIPFATED
                     KVDNPISLYAATKKTNELQAYAYSKIYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLR
                     SGSDIEIYNHGNCKRDFTYIDDIVEGIVRVMQSAPKKLVGSDNLPLAPYKVYNIGNSK
                     PENLLDFVDVLQQELIKAGVLPENYDFDSHKKLVPMQPGDVPVTYADTSDLERDFGFK
                     PKTSLREGLRKFAEWYKDYYNV"
     gene            complement(3048..4217)
                     /locus_tag="VQ846_001663"
     CDS             complement(3048..4217)
                     /locus_tag="VQ846_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET3076205.1"
                     /translation="MRLSKKYWFALTSKFLLILLTLLSSIIINRGLGVEKKGDYAYVM
                     NLVEIFYILGGVGLGQAYSTFKRNHSNEYQNKFVFLTFIQGLLFGIIGLIIVLADVPI
                     DYGGVIIVLSSLSIMRTNLTMIAVIENSIKRNKIVLGVSVVYSVMLVLLLLFHLVSIE
                     MVFLVYAINEFLRIILFLYYYNMFPVFEHISIKEFFTIYKFGFLSMILTLLITFNYSI
                     DIIMLKQMSSSYNVGIYSVAVTFSNMFLMVPDSFKEILFGDSAKRTFEKNKAINAIKV
                     SFLFMILLIIGFLSFGEFAITLLYGSSYIKAFPITLVIFLGSLSMIFFKILQPIYISQ
                     GKQSVAIKILSVSALINISLNWYLIPNYQITGAAIASAISYTVCGLFFIIDYLRN"
     gene            complement(4403..5707)
                     /locus_tag="VQ846_001664"
     CDS             complement(4403..5707)
                     /locus_tag="VQ846_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET3076206.1"
                     /translation="MKFSLQSNDIKNKIILLVGVVFSGTLILCSENNDVTIPLFSMIF
                     LSFMAMIIVKFNIFHPYVWYSSFFTIYSISYPLLYRMEYINYGYTKDLIFMQWLALAT
                     LLLTLPSKAIVLSERKINLNNNNYLLFLNNMNTFITTVAIIYLLGSGFRNKGEIYSGA
                     NIVIMLVFSLVYFMILIHTYQLFIQLSTKKWRLLNCNTNTAIVICLFSIVTGERDYMF
                     TLFILDVIILFYFHKIKRFYLVMLVPLAALLIPLSAVFKYTLLSGQVSSINTNNIWFD
                     LLDGEFVSASRNLQILILHNMGNYFEGRSFFNDIVRIFYNTGYSNQTWFMDTFFPNVH
                     STKYGFTLVGEGYVNGGYFGIVMIFMLTGFLMRFLYINAQRNIYGMLIYLYMIPIFIY
                     STRADFANILSPLLKYAILGTLVIVFINRMVLISPLDEMGGE"
     gene            complement(5824..6978)
                     /locus_tag="VQ846_001665"
     CDS             complement(5824..6978)
                     /locus_tag="VQ846_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET3076207.1"
                     /translation="MKVFIATEFRCTIYKNEIYLKPKAYFIYERYAKAFGEIVLCSRY
                     EKVESYPEGYHKANFINKFIPIEGLHQVLFGQNKNKIIEGMIDSDLIVVRIPSIIGSK
                     TADYALKIGKPYLTEIMGDAWDSYWYHSLKGKLLAPYIYAKTKSIVKNANYCIYVTEK
                     YLQDRYPNIKSNIVASNVNITSVENRSLKSRLYKLKKFNPQKISIMTTASVNVRAKGH
                     RFVLEAMKRLEIQGILLDYYLAGDGDQSFLKKKAEELGVANRIHFLGELTTSQIYEYL
                     DKVDLYIQPSLQEGLPRAVIEAMSRACPCIGSNKAGIPELLGPHCLFTPSSSQAIAGS
                     IIDFISMDKEKMVIKQFEFSKKFLSSVLNKRRSDYFAQIRRELNNSDGLD"
     gene            complement(6975..7526)
                     /locus_tag="VQ846_001666"
     CDS             complement(6975..7526)
                     /locus_tag="VQ846_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET3076208.1"
                     /translation="MNRIIKNIVGLLRANICLILLKVCRNKDLTFHYFLKFGKNVTVE
                     QESNAKLILGKKIRVNAGGVLKVRKGAKLKISDDVFLSNNCMIACRKYIDIKSGVKCG
                     PGVLIYDHDYDVSVPGGLKAKKFKTAPVMIGENVWIGANSIVLKGVSIGENSVVAAGS
                     VVTKDIPADTIFIQKRLSREMKL"
     gene            complement(7519..8655)
                     /locus_tag="VQ846_001667"
     CDS             complement(7519..8655)
                     /locus_tag="VQ846_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET3076209.1"
                     /translation="MNLQKKKIMLITNHDDDIYCFRKEVIESLVEYGYEVLVSCPYGD
                     KIELMKSIQFEYEDITIDRRGINPLKDIRLFLSYIRLLRKYRPDVVLTYTVKPNVYAS
                     LAATILGIKYINNVTGLGSVLTMGSCVRSLVLLLFRISFRKSNCVFFQNEYNMKMLKT
                     LGLILGDSKLIPGSGVNIEKYPVQQYPNGGNGIQGETIVFNFIGRILKEKGIDTYLAA
                     AQIIKSRYPKTEFNIIGFIEPTESNYELKICDLEKKGIVYYLGQQKDVIPHITRSHAI
                     IHPSVYGEGMSNVLLENASSGRVLITTDNPGCKEIVKDRETGYIFQGGNVEELVSILE
                     VFLGLENEKRKEMGLQGRMYIEQYFSRQIIIDAYLDKIKGALDE"
     gene            complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ846_001668"
     CDS             complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQ846_001668"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HET3076210.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNTLCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIAMSFARAGYKTLLFDGDIRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITQKCDASILITATGEANKRDVQKAKQQLEQTGKLFLGVVLNKLDISVDKYGVY
                     GRK"
     gene            complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ846_001669"
     CDS             complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQ846_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET3076211.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKPGLTNQDLQAGAYLVKDYREIVLSQDVLEEVISDLKLD
                     LMPKGLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIENALQMT
                     LLGVVPNLGKLK"
     gene            complement(10172..10903)
                     /locus_tag="VQ846_001670"
     CDS             complement(10172..10903)
                     /locus_tag="VQ846_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HET3076212.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            complement(10905..12350)
                     /locus_tag="VQ846_001671"
     CDS             complement(10905..12350)
                     /locus_tag="VQ846_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET3076213.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSKIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADS
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLTDGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNIPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLTVVKAAIQDVMEGR"
BASE COUNT         4238 a         2433 c         1654 g         4248 t
ORIGIN      
        1 aattcactta cttcatatta catttagcat gaaatgaaat aagcgaattc tactttgttt
       61 gcatacaatt aaaaatcaat tcctaaggat gttttaaata actgtgtaaa aggcataatt
      121 tcaccatttc ttagtttttc aatgctaaat tacatagtaa ctggagagct attagaaatc
      181 tctaacttcc tcatagttaa aaaatgacaa tgagatactt attgatttca attcattaac
      241 agaatacgaa ctagacaagt gagttcgtct ccttaacaaa cacttccaag cgtttacttt
      301 cctcattcag tcgtcgtaat ctacaactta catcatcaga taagtcatac tcaccattta
      361 gaatagatag aatatgttgt ttttcttctt cagtcttctc ttcaataagc acatttttaa
      421 aaattactgc cattatcagc acctcacttc gaaggaatat atgcaactat atcctattaa
      481 gtcaatctct actgacaaaa gaagtatatt tacttcacta agtgcgagcg agaatataga
      541 aatatcttta ttgttttaga tgagttgtcg ctatcctcca gctagttttt aactattcta
      601 tcattcaaat gatatagatt attagggact agaaatatac aaattgattt aatctctctt
      661 aaaaatatcc ctagtataaa ctttacccct cacatcttct aaagtctcat caaaacgatt
      721 tgcaacaatc acttgactag atttcttaaa tatttcaaaa tcattaacta cttgactacc
      781 atagaaagta gtaccacttt caagagttgg ttcgtaaata ataatagtaa caccttttgc
      841 tttcaaacgc ttcataatac cttgaataga agattgtcta aaattatctg aattactttt
      901 cattgttaaa cgataaattc ctacaacaat ctctttttct ttatctggtt tatagaaatc
      961 attgcctaca taagatccag caagatctaa aatacgatct gcaatataat cttttcttgt
     1021 ccgattactt tgtactatag ctgttatcat ttcttgtgga acgtcagaat aatttgcaag
     1081 aagttgcttg gtatctttag gaagacagta acccccataa ccaaaagaag gattattgta
     1141 gtgagaacct attctcggat ctaaacaaac tccatcaata atcgcttgag tatctagtcc
     1201 tttcatctca gaataagtat ctaactcgtt aaaataggaa acccggaggg caagataggt
     1261 attagcaaaa agttttactg cttcagcttc tgtaaaatcc atatacaatg tttcaatatt
     1321 ttctttcact gcaccttcct gaagcatttt tacaaaaatt tcagcagact ttaccaaatc
     1381 tttatcatct ttatctgtac ctataataat tcgactcgga tataggttat catataacgc
     1441 ttttgactcc cgtaaaaact caggactaaa cataatattg tctgtaagaa atttcctccg
     1501 tactgacttt gtatatccaa caggaacagt acttttaatt attataattg catcaggatt
     1561 atatttcata actgcttcaa tgacagcctc gactgaagat gtatcgaaaa aatttttttt
     1621 cggatcataa ttagttggag ccgcaataac tataaaatcc gcattctcat atgcttcctc
     1681 tgcattcaat gtagctacga gattcaattc tttttccgcg agatattttt ctatataatc
     1741 gtcttggata ggggacttct ttttattgat taactcaact ttctcaggaa tgatatctac
     1801 ggcaacaact tgatgatgtt gagccagtaa gattgcaata gataatccaa catatcctgt
     1861 acctgctact gctattttca taattttttc ctttctcaaa acactctcgt tatacattat
     1921 agtagtcttt ataccactct gcaaactttc tcaaaccttc tcgtaaagaa gtttttggtt
     1981 taaaaccaaa atctctctct aaatcacttg tatctgcata agtaacagga acatcgcctg
     2041 gttgcatagg aactaatttt ttatgactat caaaatcata attttccggc aaaactcctg
     2101 ccttgattag ctcctgttga agaacatcca caaaatctaa caaattttcg ggtttactat
     2161 tcccaatatt gtacacttta tatggggcta acggtaaatt atcagatccc actagtttct
     2221 tcggagctga ttgcatgacc cttactatcc cctcaacgat atcatctata taagtaaaat
     2281 ctcgtttaca attaccatga ttataaatct caatatcgga accacttcgc aatttatctg
     2341 taaatccaaa ataggccata tctgggcgcc cagcaggacc ataaactgta aaaaagcgta
     2401 agcctgtcga aggaatattg taaatctttg aataagcata cgcttgaagt tcatttgttt
     2461 ttttagtcgc agcatatagt gaaataggat tgtctacctt atcttctgtt gcaaacggga
     2521 tttttttatt tccaccatat accgatgagg acgatgcata aactaagtgt tccacaccac
     2581 catcatatat ctcatacgaa tgacgacaag cttccaacac attatgaaag ccaacgatat
     2641 tactttctat atatacatct ggatttgtaa tggagtagcg tactcctgcc tgggcagcta
     2701 gattaacaac aatctgagga tgataactct taaaaatctt attaataagc attttatctg
     2761 caatatcacc cttgataaat atccagtttt tttctggata atcctgaatt agcttattaa
     2821 gttgaagcaa tcgataatct ttaagtgcaa tatcatagta atcattcaaa ttgtccaaac
     2881 caatgattgt catcgttcct tctaattcca ataatttcct aaccagattt gatccaataa
     2941 aaccagctac tcctgtaatt agaattgttt ttcctctcat atcataatac ctcttgttat
     3001 ttttatattt tcatccacta atacacattc tttaacaact attataatca atttctaaga
     3061 taatctataa taaaaaacaa accacataca gtataagaga ttgctgatgc tatcgcagct
     3121 cctgtaattt ggtagttagg aattaagtac cagtttaaac ttatatttat caaagccgac
     3181 acagataaaa tttttatagc tacactctgt ttaccctgag atatataaat tggttgtaaa
     3241 attttaaaga aaatcatcga taagcttcct aaaaaaatga ctaatgttat aggaaatgcc
     3301 tttatataac tggaaccata aaggagcgta atcgcaaatt ctccaaaaga aagaaatcca
     3361 ataattaata aaatcataaa tagaaatgat acttttattg catttattgc tttatttttc
     3421 tcaaaagttc ttttagccga atcaccaaat aaaatctctt taaatgagtc tggaaccatc
     3481 aaaaacatat tagaaaacgt aactgcgaca ctgtatatac ctacattata actactagac
     3541 atttgcttca acattatgat atcaatacta taattaaatg taattaatag agtaagaatc
     3601 atagaaagaa atccaaactt atatatagtg aaaaattctt taatactaat atgttcaaaa
     3661 actgggaaca tattgtaata gtataaaaat aaaatgatac gtaaaaattc attaatagca
     3721 tataccaaaa ataccatctc tatagaaact aaatgaaata gtaacaacaa tactagcata
     3781 acggaatata ctacgcttac tcctaataca atcttattcc tctttatact attttctatt
     3841 actgcaatca tagtaagatt tgttctcata atagatagac tggacaaaac tattattacc
     3901 ccaccataat ctatcggaac atcagctaaa actattatta aacctattat tccaaacaat
     3961 aatccttgaa taaaagttaa aaaaacaaat ttattctgat actcattaga atgattcctt
     4021 ttaaaggtcg agtaagcctg acctaaacca actccaccta aaatataaaa aatttctact
     4081 aagttcatta cataggcata atctcctttt ttttcaacac ctaatcccct attaattata
     4141 attgaggata aaagtgttaa taagattaac aaaaatttac tcgttaaagc aaaccaatat
     4201 ttcttagata gtctcactta ttttctccgt atgttaaatt ttataacagt aaatttaaaa
     4261 ttatagtata ttgaaataga atatgaacaa aacgattagg gaatttaaag taatttataa
     4321 aaattcttta gaagctgcat tgtactactc caacttcaaa ccactatagt atcaaaaagt
     4381 ataccccacc tttttaaata aactactcac cacccatttc atccaatggt gatatcaaca
     4441 ccattctatt gataaaaaca ataaccaatg ttcccaatat agcatattta agtaatggag
     4501 ataatatgtt agcaaaatca gcacgagtag aataaataaa aataggtatc atatacagat
     4561 atatcagcat cccgtatata tttctttgag cgtttatata aagaaatctc atcaaaaagc
     4621 ctgttagcat aaaaatcatt acaattccaa agtatcctcc attcacatat ccctctccca
     4681 caagagtaaa accatatttg gttgaatgta cgtttggaaa aaaggtgtcc ataaaccaag
     4741 tttgattaga ataccccgta ttataaaaaa ttcttactat gtcattaaaa aaagaacgac
     4801 cttcgaaata attgcccata ttatgtaata tcagaatctg taaattacga ctagctgata
     4861 caaactcacc atctagcaaa tcaaaccaaa tattatttgt atttatagat gaaacttgcc
     4921 cacttaataa agtatattta aaaactgcag ataacggaat cagtagtgcc gcaagcggaa
     4981 ctaacattac aagataaaaa cgttttattt tgtgaaagta aaataatata atcacatcaa
     5041 ggatgaataa agtaaacata taatctcttt cccctgtaac gatagagaag agacagataa
     5101 ctatcgctgt gttagtatta caatttagta gtctccattt tttagtactc aactgtataa
     5161 atagctgata tgtatgaatg agaatcataa aataaaccaa agaaaatact aacattatca
     5221 caatattggc acctgaatat atttcccctt tatttctaaa accacttcct aatagatata
     5281 taattgctac tgttgtgata aacgtattca tattattgag aaataataaa tagttattgt
     5341 tatttaggtt tattttcctt tcgcttaaca caatggcttt agaaggtaga gttaacaaca
     5401 aagttgccaa agccagccat tgcatgaaaa tcaaatcttt tgtataacca tagtttatat
     5461 actccattct atagagtagc gggtaagaaa tagaataaat agtaaagaag ctactatacc
     5521 acacataggg atgaaaaata ttaaatttca caattatcat tgccataaaa cttaaaaaaa
     5581 tcattgaaaa taatggaatt gtaacatcat tattttcact gcataaaatt aaagtccctg
     5641 aaaaaactac acctactaga agtattattt tattttttat atcatttgat tgtagactaa
     5701 acttcattta cttttttcct ttaactaata atttaaacgt aatgcgtatt caaaaatttg
     5761 attggctatg tagtcagttt ttatagcata tttaatatat cgaaacggat tttccatcgc
     5821 attttaatct aaaccatcag aattatttaa ttctcttcta atttgagcaa aatagtcact
     5881 tctgcgttta tttaatacac tcgaaagaaa tttttttgag aattcaaact gttttatcac
     5941 catcttttcc ttatccatac taataaaatc tataatagat cccgcaatcg cttgtgaaga
     6001 ggatggagta aatagacaat gtggccccag caactctgga attcctgctt tattagaccc
     6061 tatacaaggg caagcacggc tcatagcttc aattaccgca cgaggtaacc cttcttgtaa
     6121 acttggttgt atatataaat caactttatc caaatactca taaatttgtg atgtagtcaa
     6181 ttcccctaaa aaatggattc tattcgctac tcccaattcc tctgctttct tttttaagaa
     6241 actttgatca ccatcacctg ctaaataata atccaacaaa ataccttgta tttctaatct
     6301 cttcattgct tccaatacaa atctatggcc cttggctcgt acattcacag atgctgttgt
     6361 cattattgaa attttttgag gattaaattt tttcaactta taaagacggc tcttcaaaga
     6421 tctattctct acagaggtaa tattaacatt tgaagcaacg atattagatt taatattagg
     6481 atatctatct tgtaaatatt tttctgtcac gtatatgcaa taattagcgt tttttacaat
     6541 tgatttagtt ttggcgtata tatatggagc taataatttt ccctttaaac tatgatacca
     6601 gtaagaatcc caagcatccc ccattatttc tgtcagatac ggcttaccta tcttcaatgc
     6661 gtagtctgca gtttttgatc caattataga cggaatacga acaactatta agtcgctatc
     6721 aatcattcct tcaataatct tatttttatt ttgaccaaat agaacctgat gtagaccttc
     6781 tatagggata aatttattaa taaagttcgc tttatgatat ccttcagggt atgattccac
     6841 cttctcataa cgactacata gtacaatctc tccaaatgct ttagcatagc gctcataaat
     6901 aaaataagct ttcggtttta aatatatttc atttttatag attgtacatc taaattcagt
     6961 cgctataaat actttcataa tttcatctcc cttgataaac gtttctgaat aaatatagta
     7021 tcagctggaa tatcctttgt tacaacactt cctgctgcaa ccacactatt ctcaccaata
     7081 ctcactccct tcaagacaat gctgttagct ccaatccaaa cattttctcc aatcataact
     7141 ggggccgtct taaatttttt tgctttcaat ccacctggaa cactaacatc atagtcatga
     7201 tcatatataa gtactccagg accacatttt actccagatt taatatctat gtatttacga
     7261 caagctatca tacaattatt actcaaaaat acatcatcag aaatcttgag ttttgctcct
     7321 tttctaactt tcaatactcc cccggcgttt actctaatct tttttcctaa gattaattta
     7381 gcattagatt cctgttcgac agttacattc tttccaaatt ttaaaaaata atgaaaagta
     7441 agatccttgt ttcgacacac ttttaataat atcaagcaaa tatttgcacg aagtaaacca
     7501 acaatatttt ttataatcct attcatctaa agctcctttt attttatcta aataagcatc
     7561 tataataatt tgacgagaaa aatattgctc tatatacatt ctcccttgaa gtcccatctc
     7621 ttttcgtttt tcattttcta gacctaaaaa aacttccaat atagagacta gttcctcaac
     7681 atttccccct tgaaatatat agcctgtctc tctatcttta acaatttctt tgcaacctgg
     7741 attatctgtc gtaattaaaa cacgtcctga actagcgttt tctagtaata cattgctcat
     7801 tccttcacca tacacactgg gatggataat tgcatgggaa cgggtaatat gaggtatcac
     7861 atctttttgt tgtcccaaat aataaacgat tccttttttt tctaagtcac aaattttaag
     7921 ttcataatta ctctctgtcg gttctataaa gccaataata ttaaactctg ttttgggata
     7981 tcgactctta ataatttggg cagcagccag ataagtatct ataccttttt cttttaatat
     8041 cctaccaata aaattaaaaa cgattgtttc tccttgaatt ccatttccac catttggata
     8101 ctgttgcaca ggatactttt caatgttcac tcctgaccca ggtatcagct tagagtcacc
     8161 caaaatcaag ccaagagttt ttaacatttt catattatat tcattttgaa aaaaaacaca
     8221 atttgatttc ctaaaagaaa ttctaaacag taataacacc aggcttctta cacaagaacc
     8281 cattgttaag acactaccta aacctgttac gttatttata tattttattc ctaaaattgt
     8341 tgcagctagg ctagcatata catttggttt cacggtatat gtcaagacta catccggtct
     8401 atattttctc aataatctta tataactaag aaatagacga atatctttta aagggtttat
     8461 tcctcttcta tcaatagtaa tatcttcata ctcaaattga atagacttca tcaactctat
     8521 cttatctcca taaggacatg acactagcac ttcataccca tactcaacta gtgactctat
     8581 aacttctttt cgaaaacaat agatatcatc gtcatgatta gttattaaca taattttttt
     8641 cttttgtaaa ttcatcaaaa taatcttcct aaaatataat taaccgctac tccattatcc
     8701 tttacaacta tcattttaaa aatagtaatt ttctataagt aattgtttcc aaattgtgac
     8761 actttaaaat tctatacaca tatagaactt acaaactatt tacgaccgta aactccatac
     8821 ttatcaaccg agatatccaa tttatttaaa acaactccca ggaacagttt ccctgtttgt
     8881 tctaattgtt gtttcgcttt ttggacatca cgtttattcg cctcacctgt tgctgttatc
     8941 aagatggacg catcacactt ttgagtgata attgccgcat caataacaat tccaataggt
     9001 ggtgtatcaa caatgatata atcaaaatat ttacgcaatg tttcaatcat atcattaaaa
     9061 tttttacttt gtaacaaggc tgtagggttt ggtgatacag atcccgattg aactacaaat
     9121 aaattttcaa tatttgtatc acataaaccg tgagataaat cagctgtccc agataaaaat
     9181 tctgttagcc ctgtaatttt ttcacgagat ttaaaaaatc ctgacataac tgaatttcga
     9241 atatcgccat cgaacaaaag agttttatag cctgcacgcg caaatgacat tgctatattt
     9301 acggaagtag ttgtttttcc ttcccccgga ttaacagaag taacggaaat tacttttagt
     9361 ttatctccgc tcaactgtat atttgtacac aaggtattgt aatattcttc tgccttctta
     9421 atgaactcca gttttttttg tgctatttct aatgtcggca tctctctctc ctatttcaac
     9481 ttacccaagt ttggtacaac tcccaaaagt gtcatctgca gtgcattttc gatatcttcc
     9541 ggacgtttca cacgagtgtc caaaagttca agaagaagaa ctataacact agttccaatc
     9601 acccctgcca aaaaaccaat tagtgtattg cgtttaatat ttggcgaaga cggtgatatc
     9661 gccggtcttg cctcctccag tgttgtcaca tcagaaacac gagtaatact gataattttt
     9721 tgagcagcta cttctcttaa agagttagcg atacggcttg cctcttcagg aactcgatca
     9781 ctaactgaaa cagagacaat acgggtatca actggtactg ttactttaat tttattagcc
     9841 aaaccttttg gcatcaaatc tagtttcaaa tcagaaataa cttcctccaa aacatcctgc
     9901 gataggacaa tttcacgata gtctttaacc aagtatgctc ctgcctgcaa gtcttgattg
     9961 gttaaaccag gcttctctcc ctgattacgg ttaactacat aaatccgagt cgtactagta
    10021 aactcaggtt tgataacaaa agtactgtag gcaaaagcaa ctgaagaagt tataattgcc
    10081 actaataaaa tgaccaactt tcttttccat aaagctctga atagttgcaa tacatcgatt
    10141 tccaaagtgt tttgttcctt catattttct cctaaattaa ttgatccatt ataatttttc
    10201 tgggattatc tacaaaaagt tctttcgctt ttttcgctcc atatttctta gcaatgatat
    10261 catatgcctg ttgcatatat ggaggtctac tgtctaaatt gtgcatgtca cttgcaacta
    10321 catgaactaa atcacgttcc aaaaaatacc gagctctctt tttcatgaat ttatattttt
    10381 caccaaagaa cttaggtttt gaaacatgat aactatttat ctgagtatag caccccatat
    10441 caatcagttc acgaacacgt ttttcgttat tctctaaagc atcataacgt tcaatatgag
    10501 caattactgg cgtgattccc aacatcaaaa tattgctcaa tcccgtatga atctgacgat
    10561 aggaagtatg catgctaaac tcaatcaagg cataacgact atcattaagg gtaggaattt
    10621 cttttttttc tagcttttct agagcatcca gagtatagta tatctctgcg ccataagcaa
    10681 tgactaaatc atctgctact tcttttgcaa tttcccgaac ctgaagaaag ttttctgcta
    10741 tcttctcttc cggagtttca aacatgccct tgcgacggtg agaggtagag acaatggttc
    10801 tcaccccctg cctgtaggat tctgccaaga gagccttgct ttcctctctt gacttgggac
    10861 catcatctac atcaaagacg atgtgcgaat ggatgtctat cattttatct accctccatc
    10921 acatcctgta tagctgcttt aactacagtt aaactactat catctatttc catcacatag
    10981 aggttactgt ctggcattgc ataagaagga agatccatcc gacctgtccc ttttaaatct
    11041 tgagaattta ctttataatt ccctccactt tccaactgag tattcactaa atctatcata
    11101 gtctcaatcg gtatatttgt ttgaagagaa tcctgcaatc cttgaagaat actactatag
    11161 tttttcaaaa cctctgtaga agttaacttc tgaataattg ctacaatgac cttttgttgg
    11221 ttgcgaccac ggtcacggtc tccatcggtt agtgagtagc gttcacgtac aaaacctaaa
    11281 gcttgctcag agtctagatg gacattccct actgggaaat ggaacttccc atgtagagct
    11341 gaaaactctt gatcattatg aacatctacc cctcccaata agtcaatcat tttcaagaaa
    11401 gaagtgaagt tcaatcgcac atagtaattg atatccactc catagagatt ttctaaggtg
    11461 tgaatggacg aatcaactcc ataaatgccc gcatgggtta atttatcttt ttgattattt
    11521 ccactatctg cgattggtac ataggcatca cgtggcgttg tggtcaagag gattttcttg
    11581 gtatctcgat tgacagtcat taggatattg acatctgatc gcgacaccga actaatagga
    11641 ccataggtgt caattccact aacatagata ttgaaagact gattcttaga cgtcttagga
    11701 gcctctactt ttttagtgaa tcccttggta taaatctttt ttatcttcga tgcgtagtct
    11761 ggatactctg actcgatgat attttcaaag acactattta ggacaatggc cttagtctcc
    11821 cctgcaatca aactcttgta agctgccaag taagacgaac tctggttgac cgtcaaatcg
    11881 gtattctgac ttgacttgat atcagctagt agtttttgaa tattttcatt atcagtccca
    11941 gtcggtgctg tcacactcgt cagttgcgta acattttcga tcttactatc tgctaaaaca
    12001 gcgacactga ttgaatattc tgagtaatta gaagtcgcat ttaaacgatt ggtcagtcca
    12061 acaaactgct gtactgcaaa gagcgacact gagctgacaa ggatagagaa cagcaacaga
    12121 aaaatagtaa acttttcagc ttttttatag ataatcaaga gtagccctac caaggcaact
    12181 agtaggacta acgcagttac cactaggtta agatatctaa aagcaaggat attgtactta
    12241 aagattaaga acaataaaaa acaaattaac aataaataaa tagtcagcaa aacgatatta
    12301 acacttcgct tcactttctg tgaacgtgat tttttaaaac gtctactcat gattaacacc
    12361 tataccttga acatcataca attatatcac ttttttacgg taatgtctac acctttattt
    12421 tcactatctg catctttaag tatcttagta gacttcccgc gaaccaaaaa tatagtaaaa
    12481 tgaaataaga ataggacaaa tcaatcagga cagtcaaatc gatttctaac aatgttttag
    12541 aatcagatgt gtactattct agtttcaata taa
//
LOCUS       DAVBNO010000053        12027 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVBNO010000053 DAVBNO010000000
VERSION     DAVBNO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.053"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(<1..207)
                     /locus_tag="VQ846_001672"
     CDS             complement(<1..207)
                     /locus_tag="VQ846_001672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076214.1"
                     /translation="MENMTNHLLLVQIRVHGYLLDFASSEGQRQKHYRLKNLPQTVEL
                     TVDDVEEDVDLTLPENRSYQEADFF"
     gene            complement(275..916)
                     /locus_tag="VQ846_001673"
     CDS             complement(275..916)
                     /locus_tag="VQ846_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3076215.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(918..2947)
                     /locus_tag="VQ846_001674"
                     /pseudo
     CDS             complement(918..2947)
                     /locus_tag="VQ846_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(3216..4115)
                     /locus_tag="VQ846_001675"
     CDS             complement(3216..4115)
                     /locus_tag="VQ846_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3076216.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLDYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAQ
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(4187..4747)
                     /gene="rnmV"
                     /locus_tag="VQ846_001676"
     CDS             complement(4187..4747)
                     /gene="rnmV"
                     /locus_tag="VQ846_001676"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET3076217.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTVIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(4747..5520)
                     /locus_tag="VQ846_001677"
     CDS             complement(4747..5520)
                     /locus_tag="VQ846_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET3076218.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKNLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAAATTANAERIFGLDSK"
     gene            complement(5825..6718)
                     /locus_tag="VQ846_001678"
     CDS             complement(5825..6718)
                     /locus_tag="VQ846_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET3076219.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLETTPGGDKGGNTGRSDANANAGGGSQAGGPVHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKNKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVAVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(7002..7136)
                     /gene="rpmH"
                     /locus_tag="VQ846_001679"
     CDS             complement(7002..7136)
                     /gene="rpmH"
                     /locus_tag="VQ846_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET3076220.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(7281..8495)
                     /locus_tag="VQ846_001680"
     CDS             complement(7281..8495)
                     /locus_tag="VQ846_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET3076221.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLGIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(8651..8944)
                     /locus_tag="VQ846_001681"
     CDS             complement(8651..8944)
                     /locus_tag="VQ846_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076222.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLIYPGFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            9106..9558
                     /locus_tag="VQ846_001682"
     CDS             9106..9558
                     /locus_tag="VQ846_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET3076223.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIVHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(9593..10981)
                     /locus_tag="VQ846_001683"
     CDS             complement(9593..10981)
                     /locus_tag="VQ846_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET3076224.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            11047..12009
                     /locus_tag="VQ846_001684"
     CDS             11047..12009
                     /locus_tag="VQ846_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET3076225.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
BASE COUNT         3435 a         2824 c         2193 g         3575 t
ORIGIN      
        1 aaaaaaatca gcttcttgat aacttcgatt ttcgggtagg gtcaaatcca catcctcctc
       61 cacatcatcc actgtcagtt caaccgtttg aggtaaattt ttcaaacgat aatgcttttg
      121 cctttgccct tcactactag caaaatcaag caaatagcca tgcactcgga tttgaaccaa
      181 aaggagatga ttggtcatat tttccatgat gaggatatct tataaaccac ctctggcact
      241 aggataatca gataccctgc gaatacggat tttttcacac atgagcaagt ttcctcatat
      301 caatgatttc atcagcctta ttccagacta ggggattatg ggtcgcaatg atgataattc
      361 gattttcatc tttcaaatcc accaagagat tcataacctc ctctgaattt tcaggatcaa
      421 gagctgctgt tggttcatct gccaaaatca agggtggatt tttcaaaata gttttcgcaa
      481 gggcaactcg ttgggcctct cccccagata aagtataaat tttttgttct aaatccaaat
      541 accctagatt aactttttct aaagccccca cttgcctttc caaacgttct acttttgaga
      601 ttttctgacc aacaaaaccc aaatccaaat tttctttgat tgattggttt tctaagaggc
      661 caaaattttg aaagagatag cccatctgat ctcgaaaata ctcacgagtg ggaatggttt
      721 ttaaatcttt cccctgatag agaacccttc ctccatctat cttttctagc tttcccaaga
      781 tattcagcag cgtcgtcttt ccgcttccac tctttccgat taaggcataa accttgccct
      841 tctccagctt gagattcaaa ccagagaaaa tcgcgcggtc attaaatttc ttttccaatc
      901 cttgaatatc aatcatctta ttttcctttc aatactgcca tgctaccagc ttcttctttt
      961 ttgtcctgcc ttactaaaat caagagggcg ttaagcgtaa acaaagctac aactagagcg
     1021 ctaatcaaac catctcttgt caaaatacta gataggacta ggccaagcaa gagaactcct
     1081 ccttgcgcca gtaaatactt gccatgaagc tcatagattg tcataccagc aagacgttta
     1141 atcataagct cccgtctgaa ctgctcaaaa tagagaagat tcatggaatc aaataacaag
     1201 atagccgtag acagggtcaa aatcgtccca ataaggagag agtaaaactc tacccgtttg
     1261 tcatttagta ccttggcaaa aatagctggc tttttaccaa gtaagaaacc ttgtgataca
     1321 gaccttgctc ttttagagct gtgatgctgt ctccatatcg atctagtttc aaggcactat
     1381 tagccgtagt tccccacaac atccctgcaa caggtcttgt tccagtcgct tgcggcgtta
     1441 gaactacaat aatcggatct tttaggtact ggcgtgttgt cttgtaccca tcattataga
     1501 ggaaacgttc ctgtcctgtt tctgtaaaag ctagacttac ctgtgggagg tagtatttct
     1561 gactcgtcac ttctacactt tcagatgaaa agttttgcag gtaatctgta aacaatcctt
     1621 ggtagtaggc agactgctct cgcaagctct caggcaagat cagcccaaac tctccctcag
     1681 acaagttttg catcttgtcc ataaactctg aagaaacggt aatcttttct tctatcagat
     1741 agcgtggtga gacatagata acattccctg acggtgtgta gtcactgaga cgattgccat
     1801 ctaggtccac ttctgctccg tctgagaaat tgtcaacatt gctcataata taaaaagagt
     1861 ctgtattggc taaccgttct tcagtaaatg tctgccactc acgattatcc ttacgcgttc
     1921 cttcttcatc ggcaaatgca ctagaccaac caaaggatag acggtaacgg tctgaggact
     1981 ggctccattt attgctagct ctctccattt cctgcatttc acggtagtgg ggtaggagag
     2041 ctgtcgcact cgatccgacc accaatacag ctaagagttg ccccaccatc atcaatgtca
     2101 tcatacgttt gagagggagt ttccctttca atagatccac cagactattt tcctgtaaac
     2161 caagtagata gacgacactt agtaaggtag aaatccctgc cagggtcaat ccataaagta
     2221 gaagagcaat gatgaccagt tgcaccgttg ccataaacaa gacaccttga taccagagaa
     2281 tccccaatcc caaaagactg gataccagca ctgagcagat aaactgtctc acatcttcta
     2341 acactggtct gagagcaact ccaaacaagc tctcaccagc tattaagcga atccctgcct
     2401 gacgaaggga tttgatccga taaatcaggg tcagactcat aaaggtcagc agaaaaatag
     2461 ctaaactcag tagcaccata ggggtggccg ccaatagtaa gactatcgaa aatggatctt
     2521 ctccattcga aacaaagcct tgatagccaa gttctttcaa ggtggtctgt aagctcactc
     2581 catccaaact tcctgacaca atcaagtaag accctaataa atcactcgtt tctgcactct
     2641 ccttggagga aatggtcaga ccttctggaa gctctccctc accataagtg gcataggtaa
     2701 agagtgtttc tccagcttca tttggctcaa cgattcgacg ggcgatgaga ctattatgct
     2761 ctaccgccaa gtgactcaaa acctctgtca cctcttcgcg actagctact ggttgattta
     2821 tcatcattcc tgtcacatca acagaaggat agttaccaaa taaaatctct tctcgatggt
     2881 caacggaagt tatcaccata taaagcgata ccaataccat tgaaatcaaa ataaataaac
     2941 gtttcatcat ttcctccaaa aagttacttg gtcacttagt gagaaggtga ccacagtcac
     3001 tgcgatgtta agaagcttat taagttcttt ctactagcaa catcttcaaa tacattatat
     3061 ccgttttttc aagaactatc aaggacatca atgtccttaa aatctaacca tttcgtgtca
     3121 tttttagctt atatctaaaa aaacagccct aaagacgaac tttagaactg ttttgatgcg
     3181 agaacaagga catttttgtc actgattcac tatgtttata aatatttttt gacatcctct
     3241 tgccattcct ctgtcaattt ctttactaga aattcatctc caattagctg cgcaaatgtt
     3301 ttagaagact gataaaatga ttcagctaaa ctaaaatctc tttcttctct taatgcttgc
     3361 ttccacaaaa acataaatac aagaggtttc ttttgataat cttgagtttt atccataatt
     3421 tcttgtaaac acgatagata atcattaaaa caatcagtta ctcctacctt ttcaagacaa
     3481 cacattcctg aaataagagt atctctaatt ataaagaagt tttctaattt agttaccttt
     3541 ttctgttcca aaagaaagag cattagcttt tcgatttgat ttataaattt ggcaagatct
     3601 tttctaatga gcatttgata aaaaaacaaa cgaatcaaca acatatcatt tgttgaataa
     3661 acttcttttt ctatgatctg aggcatatgt tcctcaagta ttaactctgc atattcaggc
     3721 cttctagttc tagaagtatc caaacacgcc tgcaatactt cacatgctaa ttgttcttct
     3781 ttaggaagat tatcaaagta tgttttataa atctcttcca aacacgcttc cttcttatcg
     3841 tactcctctt ctttaccata gattggttca cgtaaaatct ggtatttcaa ttctaaataa
     3901 gcaggaggaa gagctgaaaa atccggcata aggctataaa ctggaactcc tagccggcga
     3961 gcaatatagt ctaacttggg caaactcggt tgcgaagctc cactttcaat tcgcgacagt
     4021 tgacgaactg tcagttcttg ctcatctcca caaaaatctg gacgactaat tcctttttct
     4081 atccgaatct ctttaatttt ttgaccaatt aacatcagac gcctcctctt ctacctgctt
     4141 cgaaaaatac tgtgaaactt aaaaaattgt aacggctaca tctttcctac tcataagatt
     4201 tcatagcttc ttccacttct gccaaagtaa ccccaaacaa ctctaggcgt ttgaggagtt
     4261 gcttgccgtt ggaatagccg attcggagag tctctccgag atattctcta cgcttacggc
     4321 tgtctgcccc tgctagaaaa ccaaggcgaa tcaaatcgct acgactaatg tcaaactgac
     4381 tctcatgttc aaattgttct gtcacctgag ctagagccgt tttcaggtct tcatagctgg
     4441 catgctcaat tcccagagaa cgccccttgg tcttggactt gggaacagct tcatctcgct
     4501 tgagaaaggc atgctgaact gttggaatga ccgtcatgat catgcgccga atccgttccc
     4561 cattaaaatc tgggtctgta aagacaatga ctccatgacg ttggtgcagg cgctgaatcc
     4621 gctctatatc ctgatcattg atggcagaac ctcgagtctc ataggtctcc acatcgaaat
     4681 aacgcttgag attgaccgta tcatcacgcc cttcaaccac aattacttgg gaaattttct
     4741 ctttcattac ttgctgtcca atccaaaaat gcgttctgca tttgcagtcg ttgccgccgc
     4801 tagctcttct gtcgtcatac cacgcaagtc agcgataaag tcgaccacat agcgagtata
     4861 ggctgtttta ttttcacggc cacgcttggg tacaggtgct aagtaaggcg catctgtttc
     4921 caccaacatc ttgtccaaag gtaactcttt agctgcttct tggaggtcag ttgccttctt
     4981 gaaagtcacc actcctgaga aggaaatggt cataccaaga tccacaaact tctctgccca
     5041 ctcaagcgtc cctgaaaatg aatgcatgat accaccacga ggaccaacgc cctcactctt
     5101 gataatctca taggtatctt ccagcgcatc acgggtatgg acaacaaaag gcaaatccaa
     5161 gttcttagat agctgaatct gacggcgaaa aacctgctcc tgcacctctt tgggcgctgt
     5221 catccaatgg tagtctaagc caatttcacc taaagccaca accttggaat gttttaactt
     5281 atccaacaag taagcctcaa cttcctctgt ataagtacca gcttctgtag gatgccaacc
     5341 aatagtcgca tagagctgct catactcatc taccaactcc aaggcatgct caatcgtcgg
     5401 tttatcaaaa ccaacaatat tcatctgtgt cacacccatc tcagcagcca aggcaatttc
     5461 ttctgcctca cgacctgcaa attcttctac attcaagtgt gtatgtgtat caaaaatcat
     5521 ctcttctaac ctcgttttct atcttctatt ataccaaaaa agaggagggg cacctaattt
     5581 ttcggtttcc cctcctctct tcaataaaga gctattctgc tatcttttct atccgatatt
     5641 gcccatctcc tattccacag ttagagacag aagagattgg ctacattgat agaatattcc
     5701 tattcagcta aagtatgtga ttatcttggg ttctcacttt tctcacccat cactaggatt
     5761 gatgacttct caaaatcccc cttataatct aactttaagt agagaataga attttctagc
     5821 tgttctattt tctacgtcta gtctttcttc ctagcaaagc aaaaccactt gttgccacca
     5881 ctagaccaag actagcaagt gctgcacttg atgctgaacc tgtgttaggt aattgtttgc
     5941 cagcaggtga ctggtaggta acatcttgca ttgttcctgc agttgcttgc ggtgcacttg
     6001 ttgtcttagg agcagcatta ggagccactg gtctcttagg aactgcaacc atggcaatgg
     6061 cttgcactcc aatcgtttct gcttccttca catcttccat agttttcgca ttttcaatct
     6121 ctttgagagc tgcttgtttt tctgcttcca ctcttgctaa aagttctgct ttttctttat
     6181 cagaaagcgg tgcgcctttg atttcatcct gcttgttctt ggctgctttt tcaatggcat
     6241 ttttagctga ttctttttca gtagctaatt gcttagaagc ttgtgattga gctgagtttt
     6301 gtgaacctgt gtgaactggt ccacctgcct ggctaccacc gcctgcattc gcattagcat
     6361 ctgatcttcc agtattgcct cccttatctc cacctggtgt agtttctagc ttatactctg
     6421 gtgtagcttc ttgagttaca gattcacctt tcgcagattg ttgaactgta tcttttccta
     6481 gaatcgtcac tactgaacca tctgggaatg tgattgttgc tgtaccatct ccagctacat
     6541 tgattgtcgc tccgtctaat gctgaaccat ttgcttgtaa aatagcaacc ttaacttttt
     6601 cttcatctgt taaatgagtt atatctttta catctactct ttgagcaggt gctttaacag
     6661 ttgatttttc agcagtcggt ttagtactat cttgtgcttt tggatcttgt gctaccaatt
     6721 gatttcctgg aattgtaata gctgaaccat ctttaaagac gattgtcgct gtaccatctc
     6781 cagctacttc aactttatag taaaataaaa taataacagg acaaatcgat caggacagtc
     6841 aaatcgattt ctaacaatgt tttagaaaca gaggtgtact attctagttt caatctacta
     6901 taacatcgct tccgaatcca tttgcatcta ctgaactttt ttacaaaaat aaaaatcagt
     6961 agactcgagt tcctactgat ttttgataga attcgtttgg attatgcagc caaaactttg
     7021 cgtcctttac gacgacgagc tgccaatacg cgacgaccat ttttagttga catacggtta
     7081 cggaatccgt gtttgcgcgc acgacgaagt ttacttggtt gataagtacg tttcacgatg
     7141 aatacctcct catagatttt gtattcgttt agccggctat aaagtgatca gttactaaac
     7201 atactttact attctatctg attctttcac ttttgtcaat agctctaggc aaatgtttcc
     7261 agctttttcg aatgcaagcc ctatctacga tactgtttca agaaacgagt catcttttct
     7321 tggatttggg ctaactcatc aacacgagga aggtaaacga tacggaagtg gtctggttcc
     7381 tgccagttaa agcctcgacc atgaaccaag agaacctttt cctgcttcaa gaaatcaagg
     7441 acaaactgct catcatcatc gatacggtac atattgcggt cgatttttgg gaagatatag
     7501 agccccgcct tgggtttaac ggcagacaaa cctggaatat cttgaatggc attatagatg
     7561 aaatttcttt gctcgtagat tcgtccacca ggaagaagca attcatcgac tgattggtga
     7621 ccccccaagg aagtttgtac gacttgttgg gccaaaacgt tagagcaaag gcgcatattg
     7681 gacagcatat tgagcccttc gatatagccc ttaacatgag tcttagggcc agacaagacc
     7741 atccaaccca cacggaaacc tgctatgcgg tgggattttg acagaccatt catgctgaca
     7801 cagaagacat ctggtgccaa gctcgccaca ggcgtatgca catgtccgtc cattaccatg
     7861 cggtcataga tttcatccgc aaagataatc aaatcgtttt gacgggcaat ctcaataatc
     7921 cccagcaaga gttccttagg ataaagggct ccagttgggt tatttggatt gataaggaca
     7981 attgccttgg tattggaagt gatttttgac ttgatatcgt caatatctgg gtaccattcc
     8041 acagcttcat cacagatata gtgaacggca tttcctccag ctaggctgac agccgccgtc
     8101 catagaggat agtctggcat tggcactaag acctcatccc cattgtccaa aagcccctgc
     8161 atggacatga caatcaactc actgacacca tttccaaggt aaatatcatc aatatctacg
     8221 tttgggaatt tcttcagctg gcaatactgc atgatggcct tacgggctga gaaaatccct
     8281 ttggagtcag aatagccttc actatcacgc gcattcataa tcaagtcatg aatgacctcg
     8341 tctggcgctg taaagccaaa ttctgctgga tttcctgtat tcagacgtaa aatcttttct
     8401 ccattcgctc gcatccgcat ggcttcttcc aatacaggac cacggatatc ataagcaaca
     8461 tgctctaact tactagactt gttatattct ttcatcttgt ttcctcaatt catcaggata
     8521 gtaacattat accactagaa agagaatgcg acaagtttac tgaaatgtgt tactagtttc
     8581 gtatagaaga aaaaccttgc taaaggcgag gttaagaacg tagagatagc aggtattttg
     8641 caaagacaca tcacatacca aaagataaac ctagaaaaag caagcctaga aaggagtcat
     8701 aaagaagatt aaacatgagg aaaagccccc atatcatcaa gtaatcccaa cgcccacttc
     8761 gttttgcaac tagaaataag attagatggt agagtacatg aaagactaaa aatccaataa
     8821 aacctggata aataagcggt accaaactct ctaattgcca gatcatgatc acttctacca
     8881 agactgaaac taggataatt ccataataca ggaaatttga tttttgaaaa gagtttttca
     8941 tcattctccc ttccttcact tctttcctgc tatcctttct tttattttat catatattta
     9001 tatagatttt aagccctaat attcttttga aaaatacttt ttttgcttta cttctatagt
     9061 gaaaaggttt acaattatag taagaaaatt tcaggaggtt tcattatggc acaacgttac
     9121 caaaatatca tggtcgcaat cgatggttct aaggaagcgg acttggcttt tgtcaaggga
     9181 gttcattctg ctctacgaaa cgacgctaaa ctcaccatcg tacatgtcat tgacacacgc
     9241 gctctccaaa gcgtatccac ctttgatgct gaagtttacg aagaactcca agtcgacgct
     9301 gaaagtctga tgaaagagta cgaaaaacgt gctaaagatg ctggggtagc agatgttcat
     9361 atcgtcattg aaatgggaaa tccaaagacc ctgctagcac gtactattcc agatgccgag
     9421 gaagtggacc tcatcctcgt tggcgcaact ggtctcaacg cctttgaacg cctcttagtc
     9481 ggctcttcat ctgaatacat actccgccat gctaaggtcg atttgctggt tgtgagagaa
     9541 caagaaaaaa ccttataatc acaaagaaaa ggagccccta gctccttttt gtttacgatt
     9601 tatttctctc tttatggcgt tcgtaagcct tgagctggcg ctgcagttcc tttttaatag
     9661 caggttctgg agcatatttt tcttcccaat tatctggttt taagatttta tgggtcactg
     9721 gatcaaaatg agccttgcca tctggaaaaa ttttccccat attggcctga tggacaatat
     9781 caaaaatacg ttctgggtcc acccccatca agacaaaact gccgtaggtg aagtaaagcg
     9841 tgtcaatcaa ggcatccact tgccctatca aatcttgctg agcaggtgtc ttcttggcta
     9901 ctttacctgc tgccttatca agggcctgat gaagttgcga tacagcttga ccaaaatctt
     9961 cttcagaagg actggctgct cgaacaaact ccaccaattc ttctatttta aaaccagccc
    10021 tatgggttgc accctctaaa tcccaagctc gaggttcttc ttgggttcgt tcatccatca
    10081 tgtggtggaa ggtcttgacc ttattgaaat gatagtcacg gctgacaaag actttttctg
    10141 aagacaaaac accaaaatgt tccaaggcct tgtggatccc atcttgttga ttgctagtgg
    10201 taatgtgctt ggcagcttcc ttgacactgc tactaccatt tcccatagcg accgacatac
    10261 cgacgccagc cagcatttca aggtcattat ctgagtcacc aaaagccatg acttggttga
    10321 ggtcaaagcc atattctttc ccaactcggc gaatgccttc taatttagaa tttccctgat
    10381 tgatgacatc cgatgcaaaa ggattgctac gtgtcaattt caagtcttca aaatcagctg
    10441 ccgccttctc agattcttct ggtgtcatca gcatcaaaac ttggtagata ggctgattca
    10501 tcaggtgaag caggtcctct tccttttggg gaacaacctt gctgaccatg cgattaaaag
    10561 actgactcac cgtccgagtt aaaacagagg gaacgaagcg actaattcgt tgggaaaaag
    10621 aacccagacc aaaggacatg attttagaac ccaacatggc atccttggtc cctagagcaa
    10681 tctccgtgcc ctctttttta gcatagctaa ttagatggcg caaatgtaac ttggaaatag
    10741 ggctcgtgaa caagactctg tctttactaa agatatactg gccattatag gttaccgcaa
    10801 aatccagatc caaatcgtcc atcaattcct taacaaaaaa aggtcctcgt cctgtcgcta
    10861 cgccaactag taccccttgt tctttgacaa tcttaatcgc gtccttagtg gatttcaaaa
    10921 cactcttgcg attgttgacc aaggttccat cgatatcaaa aaaaacagct ttgacttcca
    10981 tcctattcca atctcccctt ttgtgataca atgattatac cacatttcag aaagagttag
    11041 taaatcatgc ctaagaaaat ccttgtttta catacgggtg gaactatttc catgcaggcc
    11101 gatgcttctg gcgctgttgt gacgagttca gataatccca tgaaccatgt gtccaaccca
    11161 cttgaaggaa tccaagtcca cgccttggac ttttttaacc ttccaagtcc ccatatcaaa
    11221 cccaaacata tgctggtcct ctaccagaaa attaaagagg aagcagataa ctacgatgga
    11281 gtggtgatca cacacggaac cgatacttta gaggaaacag cctatttcct tgataccatg
    11341 gaagttcccc atatgcctat cgttctaaca ggagccatgc gtagctccaa tgagctcggt
    11401 agtgatggtg tttataatta cctaagtgct ttacgagtgg ccagcgatga cagggctgct
    11461 gacaaaggag ttttggtcgt tatgaacgat gaaatccacg ctgccaagta tgtcaccaaa
    11521 acacatacga ctaatgtcag caccttccag actccaacac atggccccct cggtctcatc
    11581 atgaaacagg aaatcctcta cttcaaaaca gctgaacctc gtgttcgctt tgaccttgat
    11641 cacatacaag gtttagtccc tatcatctcg gcttatgctg gtatgacaga tgagctgatt
    11701 gatatgctgg atttagaaca cttggacggt ttgattatcc aagccttcgg agctggtaat
    11761 attcccaaag aaacggctca aaaattagaa agccttctgc aaaaaggaat cccagtcgct
    11821 ctggtatcac gatgctttaa cggtattgcc gagcctgttt atgcctacca gggtgggggc
    11881 gtacagttgc aaaaagcagg cgttttcttt gttaaagaac tcaacgccca aaaagctcgc
    11941 ttgaaactcc tcatcgccct caatgccgga ctaacaggac aggctttgaa agactatatg
    12001 gaaggctaat actcttcgaa aatctct
//
LOCUS       DAVBNO010000054        11822 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVBNO010000054 DAVBNO010000000
VERSION     DAVBNO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11822
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.054"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            389..616
                     /locus_tag="VQ846_001685"
     CDS             389..616
                     /locus_tag="VQ846_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076226.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            904..3801
                     /gene="nanA"
                     /locus_tag="VQ846_001686"
     CDS             904..3801
                     /gene="nanA"
                     /locus_tag="VQ846_001686"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET3076227.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
     gene            4816..5268
                     /locus_tag="VQ846_001687"
     CDS             4816..5268
                     /locus_tag="VQ846_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET3076228.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            5287..6624
                     /locus_tag="VQ846_001688"
     CDS             5287..6624
                     /locus_tag="VQ846_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET3076229.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            6703..7587
                     /locus_tag="VQ846_001689"
     CDS             6703..7587
                     /locus_tag="VQ846_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET3076230.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTIFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            7587..8420
                     /locus_tag="VQ846_001690"
     CDS             7587..8420
                     /locus_tag="VQ846_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET3076231.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            8438..10531
                     /gene="nanB"
                     /locus_tag="VQ846_001691"
     CDS             8438..10531
                     /gene="nanB"
                     /locus_tag="VQ846_001691"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET3076232.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            10543..11646
                     /locus_tag="VQ846_001692"
     CDS             10543..11646
                     /locus_tag="VQ846_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET3076233.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDVCKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVRLSEIVK"
BASE COUNT         3997 a         1844 c         2452 g         3529 t
ORIGIN      
        1 gtgttttgag ttgactttgt cagtcttatc tacagcctca aaacagtgtt ttgagcatca
       61 tgcggctagc ttcttagttt gctctttgat tttcattgag tataaaaaca gatgagtttc
      121 tgttttcttt ttatggacta taaatgttca gctgaaacta ctttcaagga cattattata
      181 taaaagaatt ttttgaaact aaaatctact atattacact atattgaaag cgttttaaaa
      241 atgaggtata ataaatttac taacacttat aaaaagtgat agaatctatc tttatgtata
      301 tttaaagata gattgctgta aaaatagtag tagctatgcg aaataacaga tagagagaag
      361 ggattgaagc ttagaaaagg ggaataatat gatatttaag gcattcaaga caaaaaagca
      421 gagaaaaaga caagttgaac tacttttgac agtttttttt gacagttttc tgattgattt
      481 atttcttcac ttatttggga ttgtcccctt taagctggat aagattctga ttgtgagctt
      541 gattatattt cccattattt ctacaagtat ttatgcttat gaaaagctat ttgaaaaagt
      601 gttcgataag gattgagcag gaagtatggt gtaaatagca taagctgatg tccatcattt
      661 gcttataaag agatatttta gtttaattgc agcggtgtcc tggtagataa actagattgg
      721 caggagtctg attggagaaa ggagagggga aaattggcac caatttgaga tagtttgttt
      781 agttcatttt tgtcatttaa atgaactgta gtaaaagaaa gttaataaaa gacaaactaa
      841 gtgcattttc tggagtaaat gtcttatttc agaaatcggg atatagatat agagaggatc
      901 agtatgaatc ggagtgttca agaacgtaag tgtcgttata gcattaggaa actatcggta
      961 ggagcggttt ctatgattgt aggagcagtg gtatttggaa cgtctcctgt tttagctcaa
     1021 gaaggggcaa gtgagcaacc tctggcaaat gaaactcaac tttcggggga gagctcaacc
     1081 ctaactgata cagaaaagag ccagccttct tcagagactg aactttctgg caataagcaa
     1141 gaacaagaaa ggaaagataa gcaagaagaa aaaattccaa gagattacta tgcacgagat
     1201 ttggaaaatg tcgaaacagt gatagaaaaa gaagatgttg aaaccaatgc ttcaaatggt
     1261 cagagagttg atttatcaag tgaactagat aaactaaaga aacttgaaaa cgcaacagtt
     1321 cacatggagt ttaagccaga tgccaaggct ccagcattct ataatctctt ttctgtgtca
     1381 agtgctacta aaaaagatga gtacttcact atggcagttt acaataatac tgctactcta
     1441 gaggggcgtg gttcggatgg gaaacagttt tacaataatt acaacgacgc acccttaaaa
     1501 gttaaaccag gtcagtggaa ttctgtgact ttcacagttg aaaaaccgac agcagaacta
     1561 cctaaaggcc gagtgcgcct ctacgtaaac ggggtattat ctcgaacaag tctgaaatct
     1621 ggcaatttca ttaaagatat gccagatgta acgcatgtgc aaatcggagc aaccaagcgt
     1681 gccaacaata cggtttgggg gtcaaatcta cagattcgga atctcactgt gtataatcgt
     1741 gctttaacac cagaagaggt acaaaaacgt agtcaacttt ttaaacgctc agatttagaa
     1801 aaaaaactac ctgaaggagc ggctttaaca gagaaaacgg acatattcga aagcgggcgt
     1861 aacggtaaac caaataaaga tggaatcaag agttatcgta ttccagcact tctcaagaca
     1921 gataaaggaa ctttgatcgc aggtgcagat gaacgccgtc tccattcgag tgactggggt
     1981 gatatcggta tggtcatcag acgtagtgaa gataatggta aaacttgggg tgaccgagta
     2041 accattacca acttacgtga caatccaaaa gcttctgacc catcgatcgg ttcaccagtg
     2101 aatatcgata tggtgttggt tcaagatcct gaaaccaaac gaatcttttc tatctatgac
     2161 atgttcccag aagggaaggg aatctttgga atgtcttcac aaaaagaaga agcctacaaa
     2221 aaaatcgatg gaaaaaccta tcaaatcctc tatcgtgaag gagaaaaggg agcttatacc
     2281 attcgagaaa atggtactgt ctatacacca gatggtaagg cgacagacta tcgcgttgtt
     2341 gtagatcctg ttaaaccagc ctatagcgac aagggtgatc tatacaaggg taaccaatta
     2401 ctaggcaata tctacttcac aacaaacaaa acttctccat ttagaattgc caaggatagc
     2461 tatctatgga tgtcctacag tgatgacgac gggaagacat ggtcagcgcc tcaagatatt
     2521 actccgatgg tcaaagccga ttggatgaaa ttcttgggtg taggtcctgg aacaggaatt
     2581 gtacttcgga atgggcctca caagggacgg attttgatac cggtttatac gactaataat
     2641 gtatctcact taaatggctc gcaatcttct cgtgtcatct attcagatga tcatggaaaa
     2701 acttggcatg ctggagaagc ggtcaacgat aaccgtcagg tagacggtca aaagatccac
     2761 tcttctacga tgaacaataa acgtgcgcaa aatacagaat caacggtggt acaactaaac
     2821 aatggagatg ttaaactctt tatgcgtggt ttgactggag atcttcaggt tgctacaagt
     2881 aaagacggag gagtgacttg ggagaaggat atcaaacgtt atccacaggt taaagatgtc
     2941 tatgttcaaa tgtctgctat ccatacgatg cacgaaggaa aagaatacat catcctcagt
     3001 aatgcaggtg gaccgaaacg tgaaaatggg atggtccact tggcacgtgt cgaagaaaat
     3061 ggtgagttga cttggctcaa acacaatcca attcaaaaag gagagtttgc ctataattcg
     3121 ctccaagaat taggaaatgg ggagtatggc atcttgtatg aacatactga aaaaggacaa
     3181 aatgcctata ccctatcatt tagaaaattt aattgggact ttttgagcaa agatctgatt
     3241 tctcctaccg aagcgaaagt gaagcgaact agagagatgg gcaaaggaga gatgggcaaa
     3301 ggagttattg gcttggagtt cgactcagaa gtattagtca acaaggctcc aacccttcaa
     3361 ttggcaaatg gtaaaacagc gactttccta acccagtatg atagcaagac cttgttattt
     3421 gcagtagata aggaagatat cggacaggaa attattggta tagctaaagg aagcatcgaa
     3481 agtatgcata atcttcctgt aaatctagca ggtgccagag ttcctggcgg agtaaatggt
     3541 agcaaagcag cggtgcatga agttccagaa tttacagggg gagttaatgg tacagagcca
     3601 gctgttcatg aaatcgcaga gtataaggga tctgattcgc ttgtaactct tactacaaaa
     3661 gaagattata cttacaaagc tcctcttgct cagcaggcac ttcctgaaac aggaaacaag
     3721 gagagtgacc tcctagcttc actaggacta acagctttct tccttggtct gtttacgcta
     3781 gggaaaaaga gagaacaata agagaagaat tctaaacatt tgattttgta aaaatagaag
     3841 gagatagcag gttttcaagc ctgctatctt tttttgatga cattcaagct gatacgaaat
     3901 cataagaggt ctgaaactac tttcagagta gtctgttcta taaaatatag tagattgaaa
     3961 taagatgtga acaactctat caggaaagtc aaattaattt atagaaaaat tttagcagtc
     4021 aaggtgtact gttatgtatt caatatatta tgcggctatt aaccttgtct tctcctaaaa
     4081 ttgactttct tgttttctta tcttgtccac tcgaaacaag tattgtaaga atttgattat
     4141 ttttgaaagt acttttaata tacttgatat agttaaaaaa gatttgaaac taaattccaa
     4201 attagaaaaa gacttgaaat actaaaaaaa aaaagtatac tctaattgaa aacggtaaca
     4261 aaactaattt agagaatgaa atatagagta tttctctctt aaaagttttt ggtgaaacga
     4321 gatgtagaaa ggagatttag ccaaagagtc tattagtgct agaataatag attagaatta
     4381 ttttagaaaa acgaagtgag cagcttataa attcaagtcc ccaaatagat tcatactagt
     4441 atcttttgca aaaaataaag ggcgacttcc ttcatgaata tcaatttcat ctataaggaa
     4501 ggtagctaat tgaactaact tatttattct gtttgtcgct agaaaaatca gacctccttg
     4561 tgaagattga ggagatactt aatgaaaatc aaagaagaaa ctagcaagct agtagcagat
     4621 tgcccaaaac accgctttga ggttgtagat aagactgacg tatataatcc aaggtgaagc
     4681 gactgtggtt tgaagagatt ttcaaagagt ataggctaga gagtagtgtt tttatgtcct
     4741 tctagtagaa aatgctagac agaagaatgg ggaacttgga taggaaaaat agattgagaa
     4801 aggaggttag aagagatgat tattacaaaa attagccgtt taggaactta tgtgggagta
     4861 aatccacatt ttgcaacatt aatagatttt ctagaaaaaa caggactaga aaatttaaca
     4921 gaaggttcga ttgctatcga tggtaatcga ttgtttggga attgctttac ttatctagca
     4981 gatggtcaag caggggcttt ctttgaaaca caccaaaaat atttggatat tcatttagtt
     5041 ttggaaaacg aagaagccat ggctgttaca tcgccggaaa atgtaagcgt tacccaagaa
     5101 tatgatgaag agaaagatat tgaattatac acagggaaag tggaacagtt ggttcatttg
     5161 agagctggcg aatgcctcat cacttttcca gaagatttac atcaacccaa ggttcgtata
     5221 aatgatgaac ctgtgaaaaa agttgtcttt aaagttgcga tttcttaatg tagaaagaga
     5281 agaacgatga aaaaaatgag aaagttttta tgtctagctg gaattgcgct agcggctgtt
     5341 gccttggtag cttgttcagg aaaaaaagaa gctacaacta gtactgaacc accaacagaa
     5401 ttatctggtg agattacaat gtggcactcc tttactcaag gaccccgttt agaaagtatt
     5461 caaaaatcag cagatgcttt catgcaaaag catccaaaaa cgaaaatcaa gattgaaaca
     5521 ttttcttgga atgacttcta tactaaatgg actacaggtt tagcaaatgg aaatgtgcca
     5581 gatatcagta cagctcttcc taaccaagta atggaaatgg tcaactcaga tgctttggtt
     5641 ccgttaaatg attctatcaa gcgtattgga caagataaat ttaacgaaac tgccttaaat
     5701 gaagcaaaaa tcggagatga ttactactct gttcctcttt attcacatgc acaagtcatg
     5761 tgggttagaa cagatttgtt aaaagaacat aatattgagg ttcctaaaac ttgggatcaa
     5821 ctctatgaag cttctaaaaa attgaaagaa gctggagttt atggcttgtc tgttccgttt
     5881 ggaacaaatg acttaatggc aacacgtttc ttgaacttct acgtacgtag tggtggagga
     5941 agcctcttaa caaaagatct taaagcagac ttgacaagcc aacttgctca agatggtatt
     6001 aaatactggg ttaaattgta taaagaaatc tcacctcaag attctttgaa ctttaatgtc
     6061 cttcaacaag ctaccttgtt ctatcaagga aaaacagcat ttgactttaa ctctggcttc
     6121 catatcggag gaattaatgc caacagtcct caattgattg attcgattga tgcttatcct
     6181 attccaaaaa tcaaagagtc tgataaagac caaggaattg aaacctcaaa cattccaatg
     6241 gttgtttgga aaaattcaaa acatccagaa gttgctaaag cattcttaga agcactttat
     6301 aatgaagaag actacgttaa attccttgat tcaactccag taggtatgtt gccaactatt
     6361 aaggggatta gcgattctgc agcctataaa gaaaatgaaa ctcgtaagaa atttaaacat
     6421 gctgaagaag taattactga agctgttaaa aaaggtactg ctattggtta tgaaaatgga
     6481 ccaagtgtac aagctggtat gttgactaac caacacatta ttgaacaaat gttccaagat
     6541 atcattacaa atggaacaga tcctatgaaa gcagcaaaag aagcagaaaa acaattaaat
     6601 gatttatttg aggctgttca gtagatgtaa aagactagaa aataggtggg ctagtgagct
     6661 gaaaagctct agcccaatct tgtaaaagaa gggagaagga gaatggttaa agaacgtaat
     6721 ttaactcgct ggatatttgt tttgccagct atgattatcg taggattact ctttgtttat
     6781 ccgtttttct cgagtatttt ttatagcttt accaataagc atttgattat gcctaattat
     6841 aaatttgttg gtttggctaa ctataaagct gtgctatcag atcccaactt ctttaatgcg
     6901 ttctttaatt caattaagtg gaccattttc tcattagttg gtcaagtttt agtagggttt
     6961 gtattggctt tagctcttca cagagtacgc cacttcaaga aattatatag gaccttattg
     7021 attgttcctt gggcatttcc taccatcgtt attgccttct cttggcagtg gattctaaac
     7081 ggggtttatg gctacttacc taatctaatc gtaaaattag gtttaatgga acatacacct
     7141 gcatttttga cagatagtac atgggcattc ctatgtttgg tgtttatcaa catttggttt
     7201 ggagcaccaa tgattatggt taatgtgctt tcagctttgc aaacagtacc agaagaacaa
     7261 tttgaggctg ctaagataga tggtgcttca agttggcagg tgttcaagtt tatcgtcttt
     7321 ccacatatta aagtggttgt aggacttcta gttgttttga gaactgtatg gatctttaat
     7381 aactttgaca ttatctacct cattactggt ggtggaccag ccaatgctac aacgacgctt
     7441 ccaatttttg cttacaacct gggctgggga actaaattgt tgggtcgtgc ttcagcagtt
     7501 acagtattgc tctttatctt cttggtggcg atttgcttta tctactttgc tatcatcagt
     7561 aagtgggaaa aggagggtag aaaataatga agaagaaatc cagtatttat ttagatattc
     7621 tctcacatgt acttttagtt ggtgcgacca tcgttgcaat tttcccattg gtatggatta
     7681 tcatatcttc tgtcaaaggg aaaggggaat taactcagta tccaacacga ttttggcctg
     7741 aacagtttac attagattat ttcactcatg ttatcaacga tttgcacttc attgataaca
     7801 ttcgaaacag tttaatcatt gccttggcta caacccttat tgcgattatt atttctgcta
     7861 tggcagccta tggtattgtt cgattctttc ctaaattggg agcaatcatg tcgagactac
     7921 tcgtcattac ctacattttc ccaccaattt tgttagcaat tccctattca attgccattg
     7981 ctaaagttgg gttaacaaat agtttatttg gcttgatgat ggtttatcta tcttttagtg
     8041 ttccatatgc agtttggctc ttagttggat ttttccaaac agttccaatt ggaattgaag
     8101 aagcggctag aattgatggt gcaaataaat ttgttacgtt ttataaagtt gtgctaccga
     8161 ttgtagcacc aggtattgta gcaacagcta tttatacatt tatcaatgct tggaatgaat
     8221 tcctgtatgc cttgattttg attaacaata caggaaagat gacagtagca gtagcccttc
     8281 gttcacttaa tggttcagaa atactagact ggggagatat gatggcagcg tctgttattg
     8341 tagttcttcc atcaattatt ttcttctcta tcatccaaaa taagattgca agtggattat
     8401 cagaaggatc tgtgaagtag acgaaagaag gaaaaaaatg aataaaagag gtctttattc
     8461 aaaactagga atttctgttg taggcattag tcttttaatg ggagttccca ctttgattca
     8521 tgcgaatgaa ttaaactatg gtcaactgtc catatctcct atttttcaag gaggttcata
     8581 tcaactgaac aataagagta tagatatcag ctctttgtta ttagataaat tgtctggaga
     8641 gagtcagaca gtagtaatga aatttaaagc agatgaacca aactctcttc aagctttgtt
     8701 tggcctatct aatagtaaag caggctttaa aaataattac ttttcaattt tcatgagaga
     8761 ttctggtgag ataggtgtag aaataagaga cgcccaaaag ggaataaatt atttattttc
     8821 tagaccagct tcattatggg gaaagcataa aggacaggca gttgaaaata cactagtatt
     8881 tgtatctgat tctaaagata aaacatacac aatgtatgtt aatggaatag aagtgttctc
     8941 tgaaacagtt gatacatttt tgccaatttc aaatataaat ggtatagata aggcaacact
     9001 aggagctgtt aatcgtgaag gtaaggaaca ttacctcgca aaaggaagta ttgatgaaat
     9061 cagtctattt aacaaagcaa ttagtgatca ggaagtttca aatattccct tgtcaaatcc
     9121 atttcagtta attttccaat caggagattc tactcaagct aactatttta gaataccgac
     9181 actatataca ttaagtagtg gaagagttct atcaagtatt gatgcacgtt atggtgggac
     9241 tcatgattct aaaagtaaga ttaatattgc cacttcttat agtgatgata atgggaaaac
     9301 gtggagtgag ccaatttttg ctatgaagtt taatgactat gaggagcagt tagtttactg
     9361 gccacgagat aataaattaa agaatagtca aattagtgga agtgcttcat tcatagattc
     9421 atccattgtt gaagataaaa aatctgggaa aacgatatta ctagctgatg ttatgcctgc
     9481 gggtattgga aataataatg caaataaagc cgactcaggt tttaaagaaa taaatggtca
     9541 ttattattta aaactaaaga agaatggaga taacgatttc cgttatacag ttagagaaaa
     9601 tggtgtcgtt tatgatgaaa caactaataa acctacaaat tatactataa atgataagta
     9661 tgaagttttg gagggaggaa agtctttaac agtcgaacaa tattcggttg attttgatag
     9721 tggctcttta agagaaaaac ataatggaaa acaggtccca atgaatgttt tctacaaaga
     9781 ttcgttattt aaagtgactc ctactaatta tatagcaatg acaactagtc agaatagagg
     9841 agagagttgg gaacaattta agttgttgcc tccgttctta ggagaaaaac ataatggaac
     9901 ttacttatgt cccggacaag gtttagcatt aaaatcaagt aacagattga tttttgcaac
     9961 atatactagt ggagaactaa cctatctcat ttctgatgat agtggtcaaa catggaagaa
    10021 atcctcagct tcaattccgt ttaaaaatgc aacagcagaa gcacaaatgg ttgaactgag
    10081 agatggtgtg attagaacat tctttagaac cactacaggt aagatagctt atatgactag
    10141 tagagattct ggagaaacat ggtcggaagt ttcgtatatt gatggaatcc aacaaacttc
    10201 atatggcaca caagtatctg caattaaata ctctcaatta attgatggaa aagaagcagt
    10261 cattttgagt acaccaaatt ctagaagtgg ccgtaaggga ggtcaattag ttgtcggttt
    10321 agtcaataaa gaagatgata gtattgattg gaaataccac tatgatattg atttgccttc
    10381 gtatggttat gcctattctg cgattacaga attgccaaat catcacatag gtgtactgtt
    10441 tgaaaaatat gattcgtggt cgagaaatga attgcattta agcaatgtag ttcagtatat
    10501 agatttggaa attaatgatt taacaaaata aaggagaaaa acatggttaa atacggtgtt
    10561 gttggagcag ggtattttgg agctgaattg gctcgctaca tgcaaaaaaa tgatggagca
    10621 gagattactc ttctctatga tccagataat gcagaggcga ttgcagaaga attgggagca
    10681 aaagtagcaa gttccttaga tgagttggtt tctagcgatg aagtagactg tgttatcgtc
    10741 gcaactccaa ataatcttca taaggaaccg gttattaagg ctgcacagca tggtaaaaat
    10801 gttttctgtg aaaaaccaat tgcgctttct tatcaagatt gtcgcgagat ggtagatgtg
    10861 tgtaaagaaa acaatgtaac ctttatggca ggacatatta tgaatttctt taatggtgtt
    10921 catcatgcaa aagaactcat taatcaagga gttatcggag acgttctata ttgtcataca
    10981 gctcgtaatg gttgggaaga acaacaaccg tcagtatcat ggaaaaaaat tcgtgaaaaa
    11041 tcaggtggtc acttgtatca ccacatccat gaattggatt gcgttcaatt ccttatgggg
    11101 ggcatgcctg aaactgtaac catgacaggt ggaaatgtgg cccatgaagg tgaacatttc
    11161 ggtgatgaag atgatatgat ttttgtcaat atggaatttt ctaataagcg ttttgctttg
    11221 ttagaatggg gttcagctta tcgttggggt gaacattatg tcttaatcca aggaagcaaa
    11281 ggtgccatcc gcttagactt attcaactgt aaaggaactc ttaagctaga tgggcaagaa
    11341 agctatttct tgattcacga atcgcaagaa gaagatgatg atcggactcg tatctatcat
    11401 agtacagaga tggatggagc aattgcttat ggtaaaccag gtaaacgtac tccattatgg
    11461 ctatcatctg tcattgataa agaaatgcgc tatctgcatg agattatgga aggagctcca
    11521 gtatcagaag aatttgcaaa acttttgaca ggtgaagctg ccctagaagc aattgctact
    11581 gcagatgctt gtacccagtc tatgtttgaa gatcgcaaag taagattgtc agaaattgta
    11641 aaataaattt tggtattctc ctatctatag gtcgacttgc tcctctgaaa gtacttttag
    11701 aggagctgtt tgactttgct agtttttgaa actgaaatct attatactac aaactattga
    11761 aagcgtttta attttaaggt ataataatct catagaaata aagaaaagga ggaaagagga
    11821 tg
//
LOCUS       DAVBNO010000055        11716 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVBNO010000055 DAVBNO010000000
VERSION     DAVBNO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.055"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(122..604)
                     /gene="greA"
                     /locus_tag="VQ846_001693"
     CDS             complement(122..604)
                     /gene="greA"
                     /locus_tag="VQ846_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET3076234.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(675..2330)
                     /gene="mltG"
                     /locus_tag="VQ846_001694"
     CDS             complement(675..2330)
                     /gene="mltG"
                     /locus_tag="VQ846_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET3076235.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     ISDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTINELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTDIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(2411..2905)
                     /locus_tag="VQ846_001695"
     CDS             complement(2411..2905)
                     /locus_tag="VQ846_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3076236.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(2902..3366)
                     /locus_tag="VQ846_001696"
     CDS             complement(2902..3366)
                     /locus_tag="VQ846_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3076237.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAENQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     DLDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(3376..4710)
                     /locus_tag="VQ846_001697"
     CDS             complement(3376..4710)
                     /locus_tag="VQ846_001697"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET3076238.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLIRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(4721..5338)
                     /locus_tag="VQ846_001698"
     CDS             complement(4721..5338)
                     /locus_tag="VQ846_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET3076239.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(5386..8484)
                     /locus_tag="VQ846_001699"
     CDS             complement(5386..8484)
                     /locus_tag="VQ846_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET3076240.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELSDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLSGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(8577..9743)
                     /locus_tag="VQ846_001700"
     CDS             complement(8577..9743)
                     /locus_tag="VQ846_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET3076241.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEIQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(9751..10845)
                     /locus_tag="VQ846_001701"
     CDS             complement(9751..10845)
                     /locus_tag="VQ846_001701"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET3076242.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(11003..11656)
                     /locus_tag="VQ846_001702"
     CDS             complement(11003..11656)
                     /locus_tag="VQ846_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET3076243.1"
                     /translation="MFKGQKIAFVGPSGSGKSTIVRLLERFYKPLSGDILMEQSSIYD
                     FNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRLVTDDELMKVLDLVSLGDEIRSMK
                     EGLDTEVGERGRLLSGGQSQRLQIARAYLKDAEILIFDEATANLDADSEYAIISSLYS
                     VLKEKTVVIIAHRLSTVKDVDCIFFLEEGKITGSGTHKELLENHERYARFVQEQMIE"
BASE COUNT         3253 a         2679 c         2220 g         3564 t
ORIGIN      
        1 aagtcagtaa ccatacatac ggcaaggcga cgctgacgtg gtttgaagag attttcgaag
       61 agtagcaaaa tggaaaaagg agtgagtgaa gcacatcgcc tccccactcc tttttctgtt
      121 tttaggctgt tttttcaacc ttcaagattt ttacatcata gctaccaaca ggcgtttcaa
      181 tggttgctgt atcacctgtt ttcttgccaa tcaaggcctg cccaattggg ctttcatttg
      241 aaaccttacc tgcaaaggca tccgcaccag ctgaacctac gataatataa acttcttctt
      301 cgtcctcacc aatttcttgg atggtgactg ttttaccaat cgctacttcg tcctgggcaa
      361 ctgcgtcgct attgacgatt tcagcatagc ggatttttgt ttctaagcta gagatttgtc
      421 cttcgacaaa ggcttgttca tccttagctg cttcgtactc actgttttct gaaagatcac
      481 cgtatgaacg ggcaatctta atgcgttcta ccacttctgg tcgacgaacc aatttcaatt
      541 cttctaattc tttttcaagt ttctcctttt cctcaagggt cataggatat gttttttctg
      601 ccatttttct caactttctt ctgataatat tttctaaaga aaattatgtg aagtatcaca
      661 taattttagt ttgtttagtt taatttgctg ttgacatgtt cagcgacatt gcggtcgtgg
      721 tcttcttgat tgttagcata gtagaccttg ccttctgtga catctgctac aaagtagagg
      781 ttatcgctct tagtttgatt gatgcttgac tcaatcgcat ccagacttgg actatcgact
      841 ggaccaggca tgagacctac atttttataa acattataag gtgaatcaat gtcggtatca
      901 atcgcaacat cctcagctag actgatattt tgccccagtt ttccttgggc atacaagatt
      961 gcaatattac tttgaagtgg catatcacga tttaaacgat tgtagaatac acctgcaatg
     1021 agcttacgat cttctgtctt ggcaccttct ttttcgacca aggaagcaat ggtcaacaac
     1081 tcattgatag tcaagttttt agatttgata gtactatagt aaggagatag gttcttatcc
     1141 atagcagcta acatctcatc aatcaagctc tcaatagttg tgctttcctt gatagagtat
     1201 gtagctggga aaaggtatcc ttccaaacga taacgcgcac cgctgtcttt tacaggcaaa
     1261 ctttccagta aagtaggata tttcgctact gcttgactga taaacgtctc atcttgaact
     1321 ttagccaaga aagcctccgc tgtcaaagac tctttgaagt caccttgcaa ttgacccaca
     1381 gtttgagcaa tctgatccaa ggtataacct tctggaattg tcaaagtcgc aagtacaggt
     1441 tcttgcggtt catctgttcc acctttttgc aactctttga gtaagtcttc tgtactcata
     1501 ctcttttgca aattgtagta acctgctttc aagtcggtat aatttttata cttggcataa
     1561 aaactaaaaa tcagaccatg ctttaccaaa ccagcttttt caagcgtcgt accgatttct
     1621 tgaacgtttg aaccttctgg aattccaacc gtcacatatt tcttagaatt agcatcgata
     1681 ggtaacaaag aatctagcac gtactggtaa ccaaagtaac cacctgctga gagcaaggca
     1741 aggaatacaa ggaatgaaat cacaaaagct ttcacatgtg acttcttctc tttcttaggt
     1801 ttaacgggtt ttgctccttc tctacggcta cgacgtgatg tatcgctgat gatatccact
     1861 gtttctgtag caggcgtagg tgtttctgga cctgcttgtt ctgctttttt ctctgccgtc
     1921 gtataggaaa cagctaccct tgttggggtt tcattgtatt ctctttcaag tttcttaggt
     1981 ctaacaggac ctggacctgg tcttgatcca ctttcttccg ctggagaaga aggtacatct
     2041 tgacttggat gacttggaac accaggagtt tctctttgaa tctcatctgc tggagaagct
     2101 ggtacacctt gacttgggtg agtaggcacg gtaggagctt ttctcataat ctcctctacc
     2161 gttgacaagg aatcagccat gagttcttca gctgaaggtt catttgcagg agtgcgaact
     2221 actgcctcat cttctttcag aacttcatca tagcctttta ctttttctaa atctctcaga
     2281 atctgctctt taaagcttaa tttctcttct tctcttgact tttcactcaa aagtttttcc
     2341 tccttgttga taatccataa tattatatct taaaagtcca aaaaaagcaa tctatgatac
     2401 ttttcctaac ttattttttc atttcccaga ccatatcata ccatgtttcc cctgcaaagg
     2461 ttgactggga ctggccttcg ttgacaaatc catgtttttc ataataggcg atgagatagt
     2521 catgacaggt tagattaatg ccatctctct catcttcaag agcaacttct ttcaaggctg
     2581 tcagcaattt ctgacccagt ccgagtccct gtgcctcctt ggcaatagac agacaggtca
     2641 cagagatata accaccaggc tcatgactat agtcttttat ttcttctgta aaagactggt
     2701 cttgcagatg gcggtgcagg ccaactggtc cttcgatata acccatgatt cttccttctt
     2761 tttcagcaac cagaaaagag gtctgaattt ctctcaaatg tgcttcaaag acagaaggag
     2821 gaatggcttc ttcgaccgaa aaattttcaa attcaagttc aacaatccga tccaaatctt
     2881 ctaatcttgc ttgtctgatt ttcattgttc ctccagataa aagggattaa accaaatcat
     2941 actatagccc tggctagttg catagagcaa agttgcttct tcatcaacaa aaccgttcat
     3001 ttcaaaatag gaaagcagct catcaggact ctccaaacga atccctttgt aatccagatc
     3061 aactgccacc tctttcaagg ctgcaagaag aagtgttccc aagccctgtc tctgatggtc
     3121 agactcgatg actaaagaat gtacttttag atattgcgga ttgtctgact ggggacttga
     3181 taaaatatag cctaaaagtt gattttcatc cctagctaga agaaaggtat ccgcacactt
     3241 acggatactt tcttctaaaa tatgggaaag ttgctgattt tcagctggaa aagacgaggt
     3301 ctgaagtgcc cctatctcag gcaaatcaga cttgcttgcc tgaatgatct taattggaat
     3361 ttccatggga acatcctatt gaacattgct tgtcaagtta gacaagagac gctcaaatga
     3421 gtattcatag gtttggatgt ctcctgctcc cataaagacg taaacagcat tgtcatggtc
     3481 taggagcgga gaaacatttt caacagtaat cacttggtgt tttttgttga ttttgttggc
     3541 taggtcttct accttaacgt caccatgatc tacttcacga gccgagccat aaatttgcgc
     3601 tagataaaca gcatctgctt ggtttaaagc atgggcaaag tcgtccaaca aggcaatggt
     3661 tcttgtaaag gtatgcggtt gaaagactgc tacaatttcc ttgcttgggt atttctgacg
     3721 agccgcatcc aaggtcgcaa taatttctgt tggatgatgg gcaaagtcat cgataatcac
     3781 tgtatcattg acaattttct cagtgaaacg acgtttaaca ccggaaaatg ttttcaagtg
     3841 ctcacgcacc aagttcaaat caaatcctgc tgtgtaaaga agaccaataa cggctgtcgc
     3901 attcatgata ttgtgacgac caaaggttgg aatgtggaat tgccccaagt tttgtccacg
     3961 gaaatgaacg gtgaaggttg aaccagttgt tgaacgaata agatcactag ctacaaagtc
     4021 attgccttca gcttcaaaac cataataata aattggtgca tcagacgtaa tcttacgcaa
     4081 ttcagcatct tcaccataga caaaaagacc cttggtgatt tgtttggcat agtcgttaaa
     4141 ggcattgaaa acatcctcga gacttgtgaa ataatctgga tggtcaaagt caatgttggt
     4201 gataatagag tattctgggt ggtaaggcat gaagtgacgc tcatattcgt cagattcaaa
     4261 gacaaaatat ttggcattgg ccgaaccacg acctgtccca tctccaatca agaagctggt
     4321 atctgtaatg tgagacaaga catgagacaa catacctgtc gttgaagttt ttccatgtgc
     4381 tcctgctact cccatgctaa caaagtcacg cataaagcta cctagaaact catggtaacg
     4441 tttatagctg ataccatttt ggtccgcata ggcaatttcg acgttgttat ctggacgaaa
     4501 ggcatttcca gcgataattt ccatatcacc gtctagattc ttttcatcaa aaggaagaat
     4561 ggtaattcct gcctgctcaa gaccgcgttg ggtaaagtag tacttttcaa catctgatcc
     4621 ctgaaccttg tgccccatct ggtgcaacat caaggccaag gcactcatcc ctgatccctt
     4681 aattccgata aaatgatatg tctttgacat gttttctccc ctattctgtc attctggtca
     4741 gattcaactc ttgggcaacc cgacgttctt gttctgtttg tttacttttt ttattgtaga
     4801 tttggctctt ctttagaaaa tcataattgt ttttctttgg agcaggtgct gacacttctt
     4861 cattcttggt agggatagaa tgaacttctt ccgccaagat ataatgagac tgggtcaatt
     4921 tttggctata tttgacaaat tcaccaggat tttccttttg gaaaggagtt gtcggttgat
     4981 tgccctgtct aactagactg ggctgagaat gacgtctcgc aaggctgaaa tcctgagtta
     5041 gatagttagc agagcgtttc tttttcaagt ctgcacgcgc ttcttcacgc gccacctccg
     5101 catagctctt tccttctttt ttaaccccta aaggagcttt tttaggtttc cctacttgct
     5161 tttcaatcgg ttttactggt ttttcttcag caataggagc ccattctaaa taatttttat
     5221 ctcgatactc acccttgata ttactgatca gatcagactc atcatagaga ttcatgactg
     5281 gcatttcagt caacatgacc tcgtcatctg acaccaatgg aaatcgttct tgtttcattt
     5341 tctatttcct ttcaacactt cattatagcg tattgtcttg atttttcaag tgctggcttc
     5401 agaaattccc aaaatttctc taatttctgc tagggtcaga ctaccacgtg actctgtgcc
     5461 gtccaatact tgtgacacca gatgtttctt ttgttcttgg agttcctgaa ttttttcttc
     5521 aatggttccc ttggtcacca agcgatagac ctcaaccgtt tcttcctgac ccatccgatg
     5581 ggcacggcca atggcttgcg cttccaccgc aggattccac caaaggtcaa ccaagatcac
     5641 tgtatctgca cctgacaggt tcagaccgac cccaccagcc ttgagggaaa tcagaaaggc
     5701 atctctttct ccttgattaa aggccttggt catgtcttgt ctttccttgg ctggggttga
     5761 acccgtaatt ttaaaggaag tcaggcccaa gtctgacagt tcttgttcaa ttttttccaa
     5821 cattcccttg aactgagaga aaatcaagac acggtgtccg ccgtctgcca cctgtaccag
     5881 taggtctcgg agactatcta gtttgccgct ggctccctga taatcttcca taaacagggc
     5941 aggagtgtca catatttgac gcaagcgcat caaaccagat aaaatttcca cacgacttcg
     6001 ctgaaattcc tgttctgaca cttgagccag atggtctcgc atctgttgta actgggcaag
     6061 gtaaatagcc ttttgctggt cttccagttc atttttataa accacctcaa tcaagtctgg
     6121 caattcagtc agaacttctt ctttcttgcg tcgcatcacg aaaggcttga taaactgagc
     6181 cactcgctct gctggcaatt tcataaattc tttcttgctt ggcaaaagtc cgggcatgac
     6241 gatttggaaa atagaccaca actcacccag atggttttca atcggagttc ctgacaaggc
     6301 aaagaccgac ggcaccacaa attgtctcaa ggtctgggca atcttggtct gggcattttt
     6361 catgacctga gcctcatcta agaaaaggaa gtcaaaggcc atcccctgat aaaactcact
     6421 gtcctgacgg aaggtggcat agctagtcac atagatttga tggctctcgg caagaatctc
     6481 ctcacgactt gctttcaaac catgaacaac agccacatcc aactgtgggg caaatttctg
     6541 aaactcatcc gcccagttgt aaatcaaacc cgacggagcg agaatcaaga ctcgactttc
     6601 ttttgtcact tgactagtca aaaaagcaat ggtctgaagg gttttcccaa gtcccatatc
     6661 atcagccaaa atcccaccaa aaccataatg atggagcatc tgcaaccagc caattccctt
     6721 ttcctgataa tctcgcaagt cagccttgac ctgagttgct tgcaaaggaa agtcctctgg
     6781 atgcgtcaaa tcctgggcca gattctggaa ttcttgtgaa aaagaaacac ggtctcgccc
     6841 ttcaaagaga tgagctaaac tgtaggccaa ggatttccga gcctgcaagg tcccatcttt
     6901 taattcaaat tgccccagtt cctgtagatt ttggcgaatt ttcttggttt cttcatcgaa
     6961 aaagtaaact tgattagacg aatcaatata aaaatcctga ttggcaacca aggcctgcat
     7021 ggcttggtcg atttcctcct gggcaatatt ttgaaaatca aactggattt ccaagagacc
     7081 tcccttggag gcaatctgca cctgaggact cgctaggcta taaagctctt ctagtttatc
     7141 tgataggtca acatgcccga gtttttcaaa gactggaatg atatcatgaa aaaaatgata
     7201 gacagactcc gcttttaagg cctgacgcca agattgaaaa tcggcttcaa agcccgcagc
     7261 caaacagact tggaaaattc tttcttctaa gtccgcgtca cttgaaaatg gtaattcttc
     7321 tagctcttgt cggctagata cctgtctatt tccataatca aactgaattt ctaaacgaat
     7381 ccgattatct tcttccctgt caaagtaaaa agagggcgca aaagttttga tttgtagacg
     7441 ttctggagct gaaacggtgc ccatctggat aaaaagagtc agacaggagg ccaatttgtc
     7501 tcgatcactg ctatcaaatt gcaggtattt ctttccttgt tgacccacag gtaacgcttt
     7561 aatttccttg agaagacgca tctgctggtc tgttaaaaaa taaacctgac ctttatggaa
     7621 aagtactgct ccctgataaa agacattgac cctaggactc tcactgattt ccatttcaaa
     7681 atagtccgag tattctgtta ctgtaaaggc aaatagattg gcatcagcat gcatatcctg
     7741 aaaaagcagg gtttggtagc tatccacttg atggtcaaat tgaaaatggg gcaaggccat
     7801 cagtaaattc acaccctgct caaaaaaggt cagagggaaa aagaggtgcc gaccttggtt
     7861 ttggaaaaag aggtctggaa ccagcccttc ctccgttagt ccgtgcaaga aagtcaaaag
     7921 ttcttggctg gcatcatcaa aggcttccca agaaagagac tcctcataaa tcttgccaat
     7981 catatacgac tttctctgct cgacaatcct taaaaaaagt ggaatatccc gaatgacata
     8041 gtatttttgg ctattgattt ggccgattct cagagtccac aagatatgat tggttcctgc
     8101 ttccacctga cccacagctg ataactcata ggcgcattct gattttggag ataaaattcg
     8161 attcaaaaac ttgccaccca aggtcacctt ggtttcaacg gcctcttttt cttcatgacc
     8221 ttcttccaga ctccacaaga tttcctgacc acgctcatca tttttcagaa aatgctctag
     8281 cgctgccaaa tgcacacagt agcccctctt ttgaaaagaa tcacaggcac aaaaaaccaa
     8341 atcatcctct aaactatagc gcagttcttc ttctgcaacg cgagcgtaga gccgattgtt
     8401 cttttccttg atgatatcaa ccttaccagt ttcataaagg gcaacacctt cgatacgaat
     8461 tttccccgga atcaatttag ccatattttc acctttacct tatcttttta ttataccata
     8521 ttttcgccta tgaaaatagc cttctaggaa gacttttctc ctagaaggct ggatttttaa
     8581 cgtttggcaa aagtagccac aatccgctga cagacttctt gcaacagaga tttgggcata
     8641 gctatattga tgcgggcatg gagacttcct tcctctccaa aatccaaacc acggttgagg
     8701 ataaccttgg cttcatttct caacaactct tgcaatgttt catcagtcag gtcataggct
     8761 gaaaagtcaa gccaaatcaa gtaggtacct tgcggtttca tgaccttgat tttagtctct
     8821 tttccaaata gatccaccac ataattgatg tggtcttcaa agacttgctt gagttcctct
     8881 agccaatctt taccgtatcg ataggcagct tctgtcgcca aataacccaa gcctgaaatt
     8941 tcatgctgat tattggccaa caggcgtttc tggaaagcca gtctcaactt aggattttca
     9001 atgactgcat aggaattttt tgttccagca atattaaatg ttttagtggc actgctcaag
     9061 acgatagcaa aatttttgaa ggcaggattg atggtattga aagactggtg tttgtgacca
     9121 aagagggtca aatcttggtg aatctcatcc gaaactaaca aaacaccgtg tttttggcaa
     9181 agttggccaa tcttctccaa cacttctttt tcccaaacac gtccaccagg attgtgaggg
     9241 ttgcaaagaa tatagagttt aacctcctct tccaccaaat ccttttcaag ttggtcaaag
     9301 tcaatctcaa acagaccatc cttttccact aaggaattag taatcaatct acgattattc
     9361 aacttgacac tgcgagcaaa gggtgggtag acaggcgtgt taattaaaac cgcctcgcct
     9421 tcttttgtaa aggtttgaat agctgttgag atggctggta ccacaccctc gataaagaca
     9481 agagcctctt tgtcaaagtt gtaaccgtat tgtgtagctt cccacttttg aatttcctta
     9541 attaagtctt cactggcata ggtataacca taaaccagtt ggtctgcgta agtttgcacg
     9601 gcttggcgga tttcaggcaa gaccacaaag tccatatccg ctatccaagc tggtagaact
     9661 tcactatccg tttctgtttc tttccattta taggtatggt gccctaaacg gttgggcagg
     9721 cttgtaaaat catattttcc catctttgtc ttatccttct aaggcttggc gcaaatctgc
     9781 aatcaaatct ctagcatcct caatcccaat agacaaacgc aagaggtcat ctgtcaaacc
     9841 ataagaatgg cgtacttctg ctggaatatc agcatgagtt tgagtcgttg gataagtaat
     9901 aagactttcc actccgccca aactttccgc aaaagagaag accttgagac tgttcaaaat
     9961 atgaggaatg cgtgtttcat cggctacttt aaaggaaatc atgcctccac gaccagtgta
    10021 gagaacttcc ttaactgctg gagaatcctt caaaaaggca accacttctt gggcgttagc
    10081 tgttgagcgc tccatacgaa gagataaggt cttgagacca cgaagcaact ggtagctgtc
    10141 aaatggagac aagactgccc ctgttgtatt gagattgtaa aaaagcttct cgtatagttc
    10201 taaactattg gtcacaacca ctccagccaa gacatcattg tggcctgcta gatacttggt
    10261 tgctgaatgg agaacgatat ctgctccatc ttcaatcgga cgttggtaga tagggctata
    10321 gaaggtattg tccaccacca ctttggcacc cttagcatga gccaattttg ctagtttttc
    10381 gatatcaaat tccaacatca agggattggt tggggtttcg atatagagaa catccacatc
    10441 cttttctaac tcggcaatca actcttcttc tgtattggca taggtaaaat ggaaacgacc
    10501 ttcctgctcc acttggttaa accagcgaaa agaaccaccg taaagatcac gcactgccaa
    10561 gaccttactt cctactggaa agacgctaaa ggccagtaca atagctgaca tccctgagct
    10621 agtcgctagg gcatagtctg ctgactcaat agccgccaag acttcctcag ccttactacg
    10681 agttggattt ttagtgcgcg tatagtcaaa cccagtagat cgaccaaact ctggatgctg
    10741 ataggtcgtt gaaaaatgaa gcggtgtcac caatgcacct gtcgcttcat ctgacttaat
    10801 acccgcctgg gccaaaattg tgttaatgtg taattccttg ctcatacaat acctccaaat
    10861 ctatagtaac tattgtacca cttattttca tcaactcatt ttcttctttt caagagctag
    10921 ttatagtttc aaactatata aaaagggagt ttttcctgct ccctttaata gactataaaa
    10981 tggtgaatct caaaagacac cttcactcta tcatttgctc ctgcacaaaa cgagcataac
    11041 gctcatgatt ttccagtagt tccttatgag ttcctgagcc agtgattttc ccctcctcta
    11101 agaagaaaat acaatccaca tcttttaccg ttgacaaacg atgcgctata atcacaaccg
    11161 tcttctcctt taatacagaa tagaggctac tgataatcgc atactcagaa tccgcatcaa
    11221 gattagcagt ggcttcatca aatataagaa tttcagcatc ttttaagtag gctctagcta
    11281 tttgaagtct ttggctttgc ccccctgaca agagtcgtcc gcgttcacca acttcagtat
    11341 ctagtccctc tttcatggag cgaatctcat cacctagtga tactaagtct agcactttca
    11401 tcaattcatc atcagttact aagcgattca aaccgagaca aagattgtca cgaatactgc
    11461 cagataagac tgcattattt tgtgaaaccc aagcgatttt acttctccat tcttttaagt
    11521 taaaatcata tatacttgat tgctccatta gaatatctcc tgaaagcggt ttataaaacc
    11581 gctctaacaa acgcacaatc gttgattttc ctgatccaga tggtccaaca aaagcaattt
    11641 tttgcccctt gaaaattgaa caagtaatat cctttaagac aggtcgattt tcatcataac
    11701 caaaatagac atggtt
//
LOCUS       DAVBNO010000056        11698 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVBNO010000056 DAVBNO010000000
VERSION     DAVBNO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.056"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(1..162)
                     /locus_tag="VQ846_001703"
                     /pseudo
     CDS             complement(1..162)
                     /locus_tag="VQ846_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            148..633
                     /locus_tag="VQ846_001704"
     CDS             148..633
                     /locus_tag="VQ846_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET3076244.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAESPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            717..1424
                     /locus_tag="VQ846_001705"
     CDS             717..1424
                     /locus_tag="VQ846_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3076245.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGIEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGKQEGDDSDNIADNSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            1425..2756
                     /locus_tag="VQ846_001706"
     CDS             1425..2756
                     /locus_tag="VQ846_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET3076246.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            2923..3798
                     /locus_tag="VQ846_001707"
     CDS             2923..3798
                     /locus_tag="VQ846_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET3076247.1"
                     /translation="MKFKKILTLAAIGLSGFGLVACGNQSSTSKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            3916..4779
                     /gene="pstC"
                     /locus_tag="VQ846_001708"
     CDS             3916..4779
                     /gene="pstC"
                     /locus_tag="VQ846_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET3076248.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            4772..5587
                     /gene="pstA"
                     /locus_tag="VQ846_001709"
     CDS             4772..5587
                     /gene="pstA"
                     /locus_tag="VQ846_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET3076249.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            5589..6341
                     /gene="pstB"
                     /locus_tag="VQ846_001710"
     CDS             5589..6341
                     /gene="pstB"
                     /locus_tag="VQ846_001710"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET3076250.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            6356..7006
                     /gene="phoU"
                     /locus_tag="VQ846_001711"
     CDS             6356..7006
                     /gene="phoU"
                     /locus_tag="VQ846_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET3076251.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            7082..7507
                     /locus_tag="VQ846_001712"
     CDS             7082..7507
                     /locus_tag="VQ846_001712"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3076252.1"
                     /translation="MTNLSSVDSEELFQFYREQRNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKVLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYKARGQGKTCLTE"
     gene            complement(7681..8061)
                     /locus_tag="VQ846_001713"
     CDS             complement(7681..8061)
                     /locus_tag="VQ846_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET3076253.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGGEDYKLMPSYIELDKECLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            8273..9289
                     /locus_tag="VQ846_001714"
     CDS             8273..9289
                     /locus_tag="VQ846_001714"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET3076254.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            9311..10210
                     /gene="galU"
                     /locus_tag="VQ846_001715"
     CDS             9311..10210
                     /gene="galU"
                     /locus_tag="VQ846_001715"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET3076255.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            10628..11560
                     /locus_tag="VQ846_001716"
     CDS             10628..11560
                     /locus_tag="VQ846_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076256.1"
                     /translation="MENKFVEFIENNKKVIISSVAVGVVLILGFGWYSYNQQQAEQQA
                     KIVQLEKDSKSDKEQVDKLFESFDASSDESISKLKELSETSLKTDAGKDYLNNKVKES
                     SKAIVDFHLQKGLAYDVKDSDEKFKDKATLETNVKEITKQIDFIKKVDETFKQENLEE
                     TLKSLNDLVDKYQKQIELLKKEEEKAAEKAKESSSQSNSSGSASNESYNGSSNSNVDY
                     SSSEQTNGYSNNYGGQDYSGSGNSSTNGGSSEQYSSRNSNIGANNGYTEYRSIDASGH
                     VRDTYHNRSNGDVFDENGNYLGNLGGGIAEPPQR"
BASE COUNT         3632 a         2242 c         2295 g         3529 t
ORIGIN      
        1 catttctttt taccagaatc attcgctaat tcttctttta gggcgattga tttgtacttc
       61 cgtcgcatca atcattaccg tgtcctcaga actgagagga gttcttgaaa tcgtaaaacc
      121 actttgaaca agagttactt caacccattg gctccgacgg ataaagaaga cttgataaga
      181 actctagagt tagctcccca tgttgaaggt ggctatttta gacaaacaaa aaaagcttca
      241 gacactataa accttgctgg taagggaagg gcgctttata ccagtattta tttcttatta
      301 gaagaaacga atccttcaca ttttcaccga ttgacagcag atgaaatctg gtattttcat
      361 gcagaatccc cacttactgt tcacatgatt acagcagatg gtcactacga agcagtcact
      421 ttaggcctgg acatatccaa agggcaacaa cttcactact gtgttcctaa gggcactatt
      481 tggggatcaa cagttgataa ggatgatgct ctggtttctt gtcttgttgc tcctggtttt
      541 gaatttgagg attttgaatt atttgaaagg gtagatctac tggcgaccta tccagagcac
      601 aaggagatga ttgagcgttt gaccagatac tagtctttat aagcaaattt aatctgattt
      661 agcaatgtta ggatttctga taattgtgtt ataatgttac caataggaga taatagatga
      721 caaaacaagt cttattagtg gatgatgaag aacacattct gaaattgctt gactaccatt
      781 taagtaagga aggcttttct actcaattgg taacaaatgg acggaaggcc ttagctttgg
      841 cagaaacaga accctttgat tttatcttgc ttgatatcat gttaccacaa ttagatggca
      901 tagaagtttg taagcggctg agagccaaag gcgtcaaaac tccaattatg atggtttctg
      961 cgaaaagtga tgaatttgat aaggttttgg ccttggaatt aggggctgat gactacctga
     1021 ccaagccttt tagccctaga gaattactgg cgcgtgtcaa ggctgtcctc aggcgaacta
     1081 aaggaaaaca agaaggagat gattcagata atatcgctga caattcttgg ctatttggga
     1141 ccttgaaagt ataccctgag cgtcatgaag tctacaaggc gaataagtta ctgagtttga
     1201 ccccaaaaga atttgaactc ttgctctatc ttatgaaaca tcccaacatg acactgacta
     1261 gagagcgtct tttggaacgt atctgggggt atgactttgg gcaggaaaca cgtttggtgg
     1321 acgttcatat tggtaagttg agggaaaaaa ttgaagacaa tcctaaagcc cctcaattta
     1381 ttcgaaccat tcggggttat ggttataagt tcaaggagtt atagatgaaa cgctaccttc
     1441 aattttggct agtcaatcta agtgttagcc tcattctgat tgcagggatg gcattaactt
     1501 ggatcagtaa aggcatcggt ctctttcttc tagccctttc tttgggacta ggtggctact
     1561 ggctcttttg cctttggaaa tgggaagttg cttttgagac cttgcaccaa cctctcttga
     1621 ccagtagtga atacttttta gaaaaagggc aagaagactt aaagtctttg gctcagtatg
     1681 tgtctggcct aaaaaccaaa gtctcccaac aggatcagca atacaaggac ctagccgaaa
     1741 caatggaggt tcttctgtcg cacctaacca tggggacgtt tttagtttcc gctcaaggtc
     1801 agatgttatt atccagtcgc tctctgcctc attattttcc cgatgtggac ggcgacataa
     1861 gttcacttga tgacctcaaa cggatggata ttcggaattt agttcaccaa gcctttgatc
     1921 aaaaaacaag gttaaaacaa gaagtaagtg ggtttcatga gggtgacttg attttagaag
     1981 tgacagcagt tcccgttttt agccccaccc aatctgtgga agctgtgctc gtcttgctat
     2041 atgatttaac aacgattaga acttatgaaa agttaaattt agcctttgtc tcaaatgcct
     2101 cccatgaatt gaggacacca gttacttcga ttaagggctt tgctgaaacc attaagggga
     2161 tgtcagctga agaagaagcg ctcaaggatg actttctaga cattatttac aaagaaagct
     2221 tgcgtcttga gcatattgtt gagcatcttc ttaccttatc taaggctcaa caaatgccta
     2281 tacaatggac gaccctttct ttggcagaat ttgtacagga tttgactcaa agcttgcaac
     2341 ctcagctcaa gaagaaggat ttacagctaa aggtgcaggt gccagatgat gtcaccctcg
     2401 tatcagatag tcaattactt tcccaaatct tactcaatct tttatccaat gccatccgtt
     2461 acactgaaca agggggaaaa attgaggtca agacccaaaa ggtgaacgaa ggcattaaga
     2521 tttctgtatc agatacaggg attggtatta gtcaattaga gcaggatcgt atttttgaac
     2581 gtttttaccg agttaataaa ggtcgaagca gacaaactgg tggcactggt cttggccttg
     2641 ccattgtcaa agaactcagt caattattag gtggccaagt cacggtgacg agtcagcttg
     2701 gcagaggcag ttgcttcacg atttttcttc ctaaccaatc tttcgcacag gactaataat
     2761 ttttaaagct atttttagga gcagccattt ttcaaaagtg gctttttttg attctttaca
     2821 caatctttac atgaatcagg gataaacctt tacaaagact tgttagacta taaatgtagt
     2881 aagcctacac aagaaaaata catagagata aaggtgatta ttatgaaatt caaaaaaata
     2941 cttactcttg cagccattgg cttatcagga tttgggcttg ttgcctgtgg caatcagtca
     3001 tctacttcca aacagtcagc ttcaggaacg attgaggtga tttcacgaga aaatggctct
     3061 gggacacggg gtgccttcac agaaatcaca gggattctca aaaaagacgg tgataaaaaa
     3121 attgacaaca ctgccaaaac agctgtgatt caaaatagta cagaaggtgt tctctcagca
     3181 gttcaaggga atgctaatgc tatcggctac atctccttgg gatctttaac gaaatctgtc
     3241 aaggctttag agattgatgg tgtcaaggct agtcgagaca cagttttaga tggtgaatac
     3301 cctcttcaac gtcccttcaa cattgtttgg tcttctaatc tttccaagct aggtcaagat
     3361 tttatcagct ttatccactc caaacaaggt caacaagtgg tcacagataa taaatttatt
     3421 gaagctaaaa ctgaaaccac agaatataca agccaacact tatcaggcaa gttgtctgtt
     3481 gtaggttcca cttcagtatc ttctttaatg gaaaaattag cagaagctta taaaaaagaa
     3541 aatccagaag ttacgattga tattacctct aatgggtctt cagcaggtat taccgctgtt
     3601 aaggagaaaa ccgctgatat tggtatggtt tctagggaat taactcctga agaaggtaag
     3661 agtctcaccc atgatgctat tgctttagac ggtattgctg ttgtggtcaa taatgacaat
     3721 aaggcaagcc aagtcagtat ggctgaactt gcagacgttt ttagtggcaa attaaccacc
     3781 tgggacaaga ttaaataaaa tgtttgctcc ataaatctct aaagagatgc agacgtttca
     3841 tcgtacaata agataaagaa ggcaagtagg gaggtgtcgt atctccctta ctttcttcac
     3901 tagaaaggac aagatgtgac aaaacaagcc ttcaaagaag cagtttttag ggcaattttt
     3961 ttcatgagtg caacagtagc tgttgtagct attttgctaa tctgtttctt tatttttagt
     4021 aatggcttac ctttcatggc taactacggc tttgcccgtt ttttattagg cagtgattgg
     4081 tcgccaacga acattccggc aagctatggt attttaccaa tgatcgttgg ttccttatta
     4141 attaccttag gagcgattgt gattggggtg ccaacaggca tcttgacatc ggtgtttatg
     4201 gtttattatt gtccaaagcc cgtctatggc ttcttaaaat cagctatcaa cttgatggca
     4261 gccattccat ctattgttta tggttttttc ggcctacaat tattggtgcc ttggattaga
     4321 acctttttag gaaatggcat gagtgtccta accgcttcgt tactattagg aataatgatt
     4381 ttgccaacca ttatcagttt gtcagaatct gctatccgaa cagttcccaa aacgtattat
     4441 tctggtagct tggctctagg agctagtcat gaacggagta tttttagtgt catcttgcca
     4501 gctgcgagat ctggtatttt atcagcagtt attttaggaa tcggtcgcgc agtaggtgaa
     4561 accatggcag ttattttggt ggcaggcaac cagccaatta ttccaagtgg actcttttca
     4621 ggaaccagaa ccttaacaac caatattgtt ctggaaatgg cttacgcatc aggtcagcat
     4681 agggaagccc ttattgcaac ctcagcagtt ctctttttcc ttattctctt gattaatgcc
     4741 tactttgcct acttgaaagg aaaatcatct tatgagtaaa tacctgctaa aacttctcgt
     4801 ttattgtttt tcagctttaa cctttggctc tctcttttta atcattggtt ttatcctcat
     4861 caaaggctta cctcatctaa gtctatccct cttttcttgg acttatactt ctgagaacat
     4921 ttcccttatg ccagcgatta tttccaccgt tattctggtt tttggtgctc ttcttttagc
     4981 tttgcccata gggatttttg ctggttttta tcttgtggaa tatacaaaaa aagattccct
     5041 ttgtgttaaa atcatgcgat tggcctcaga taccttgtct gggattcctt ccattgtttt
     5101 tggtctgttt ggcatgctct tctttgtagt cttcttaggt tttcaatact ctctgttatc
     5161 aggaatctta acctcagtta tcatggtgtt gccagtcatt attcgctcaa cagaagaagc
     5221 ccttttatct gttagtgata gcatgcgtca agcaagttat ggacttgggg caggtaagtt
     5281 acggactgtt tttagaattg ttctaccagt tgccatgcca ggtattttag ctggagtgat
     5341 actagctatt ggccgtatcg ttggtgaaac agctgccctc atgtatacat taggtacctc
     5401 taccaatacg ccaagtagtc tcatgtcttc aggccgttct ctagccctac atatgtatat
     5461 gctgtcaagt gaggggctac atgtcaatga agcctatgct accggcgtga ttttaattat
     5521 tactgtttta atgataaata ctctatcaag cttattatct cggaaacttg tgaaaggagc
     5581 ttcctagtat gggaacattt tcagtcagac acctagactt attttacggg gattttcaag
     5641 ccttaaaaaa tatttcgatt caattaccag aaagacagat tactgccttg ataggcccat
     5701 ctggttgtgg caaatcaact tttctaaaaa cccttaaccg gatgaacgat ttggttcctt
     5761 cttgccatat tgaaggccaa gtcctcttag atgagcaaga tatttatagt agcaaattca
     5821 accttaatca gctacgtaag cgtgtaggga tggtttttca acagcctaat ccctttgcca
     5881 tgtctatcta tgataacgtg gcttatggcc caaggacaca tggtattcga gacaaaaaac
     5941 aattagatgc cttagtggag aaatctttaa aaggggcagc catttgggaa gaagtcaaag
     6001 atgatcttaa aaagagtgcc atgtccttat ctggcggtca gcagcaacgc ctttgcattg
     6061 cgcgagcttt agcagtagaa cctgatattc tgttaatgga tgagccgact tcagccttag
     6121 accctatctc cactttaaaa attgaagacc tcattcagca actaaaaaaa gattatacga
     6181 ttatcattgt tacccataac atgcaacaag cttcacgtat ttcagataaa actgcttttt
     6241 tcttaacagg agaaatttgc gaatttggag ataccgttga cgtgtttacc aatccaaaag
     6301 atcagcgcac agaagactat atttcaggac ggttcggata aggaaggaaa aacctatgag
     6361 aaatcaattt gacttagaat tgcatgaatt agaacaatcc tttttaggac tagggcaact
     6421 tgtccttgaa acagcttcaa aagccttact ggccttagcc tccaaagaca aggagatggc
     6481 agagctaatt atcaataagg atcatgctat caaccaaggt caaagcgcta tcgaattgac
     6541 ctgtgcccgt ttgttggcct tgcagcagcc acaagtgtct gaccttcgat ttgtgattag
     6601 catcatgtct tcttgttcag accttgaacg tatgggagac catatggcag gcattgccaa
     6661 agctgttttg caactaaaag aaaatcaact agcccctgac gaagaacagt tacaccaaat
     6721 gggtaaatta tccctcagca tgctagccga tttattggtt gcctttcctt tgcaccaagc
     6781 ctcaaaagct attagtattg ctcaaaaaga tgaacagatt gaccaatatt attatgcctt
     6841 atcaaaggaa atcattggac ttatgaaaga ccaagaaacc tcaattccca atggaacaca
     6901 atacctttat atcatagggc atctggaacg cttcgctgat tacattgcta acatttgtga
     6961 acgcctagtc tacctagaaa caggagaact agtggatttg aattaattca actaatcctt
     7021 taatccttaa aagagaagag tacgattaag tgctcttttt tatggttgta aaaaagttca
     7081 tttgaccaat ttaagcagtg tagatagtga ggagctgttt caattctatc gtgaacaaag
     7141 gaatgctgaa aactttatca aagaaaggaa agcaggattc tttggggata agacagatag
     7201 ttcgaccatg attaagaatg aagtacgtat gatgatgggc tgtctggctt ataatctcta
     7261 cctcttttta aagcagctag ctggtgatga agtaaaggtc ttgactatca agcgttttcg
     7321 acgtctcttc cttcatattg ccggaaaata tgtctctact gctagacgac atattctcaa
     7381 attctcaagt ctatacgcct attcaaaaca gtttcaagcc ttatttgata caatctgcca
     7441 gataaatctg atactccctg ttccatataa agctagaggg caggggaaaa catgcctaac
     7501 agaataagtc accttatttt aaaaatcgag catcaaacca agagaggagt ctgccctttt
     7561 ttaggaaaaa atcaagacaa atctcctcaa ttatgtctcg aacatcagaa attaagcaaa
     7621 atcaccagaa ggacagtatt tcaactagct tttctggtaa tttttgaact gtgtagttcg
     7681 ctagtgccag atatgaataa tttgggatga taaatctttc ttcctcaggt agcctatcat
     7741 aatactcttc aaaaatctta tcaaaacact ctctttcttt tgggcgatag tttcatcttc
     7801 gtatgtagga gtcctcatca agaaatactt caattctagg cattccttat ccaactctat
     7861 ataacttggc atcaacttgt aatcttcacc ccccaaacgt tcagcgatat attttaactt
     7921 tgttagtgtt ggtctggatt ctccattttc aattctaatt aattgacgga tacttaattc
     7981 agactcatca ccacaaaatt ctgaacgact gattttttta gccaaacgta atcttttaat
     8041 tttttcgcca aactctcgca acctacaaga acctcctgag ttgtttacct ctatttatag
     8101 catatactga atcaaactat ctatcagatt tcttctcact ttaactaaag actaagagtt
     8161 tatcccttcg tctcggtttt tgtgtatttt tccaccatac cccagtaatg caagtgcaaa
     8221 atcccctaga atatgataga ataagagaaa gaactctatc aaggaggaaa tcatggaaaa
     8281 acaaaccgtc gccgtcttgg ggcctggttc ttggggaacc gccctttcac aagtcttaaa
     8341 tgacaatgga cacgaggtac gtatttgggg aaatcttccc gagcaaatca atgaaattaa
     8401 tacacaccat actaataagc actactttaa agatgtcgtt ctagacgaaa atatcattgc
     8461 ctacaccgac ttagcagaaa cattgaaaga tgtggatgcg attttgtttg ttgtcccaac
     8521 aaaagtgaca cgacttgttg cccagcaagt tgcacaaacc ttggaccata aggttatcat
     8581 catgcacgca tcaaagggat tagaacctga tagccataaa cgattatcaa ccattcttga
     8641 agaagaaatt cctgaacatc tccgtagtga tatcgtcgtt gtttcagggc ctagtcatgc
     8701 agaagagacc attgtgcgtg acctaacttt aataactgct gcttctaaag atttacaaac
     8761 agctcaatac gttcaggagc tatttagtaa tcactacttc cgactttata ccaatacgga
     8821 tgttatcggg gttgaaactg ctggtgctct taaaaatatt attgctgtcg gtgctggagc
     8881 tttacatggt cttggatttg gtgataatgc taaggcagcc atcatcgctc gaggtttagc
     8941 agaaatcacc cgcctagggg tagcactcgg ggccagtcca ttgacctata gcggcttatc
     9001 tggtgtggga gatttgatcg taacgggaac ttccatccac tctcgtaact ggagagctgg
     9061 agatgctctc ggacgaggag aatccctagc tgatatagaa gctaatatgg gcatggtaat
     9121 cgaaggaatt tcaacgactc gagcagccta tgaactagcc caagaacttg gagtctatat
     9181 gcccattaca caggctattt accaagttat ttatcacgga accaatatca aagatgccat
     9241 ttatgacatc atgaacaatg aatttaaagc agaaaatgag tggtcttaac cctctataga
     9301 aaggattttt atgacatcaa aagttagaaa ggcagtcatc cctgctgctg gactaggaac
     9361 tcgattttta ccagcaacca aggcccttgc caaagaaatg ttgccaatcg tagacaaacc
     9421 aactatccag tttatcgtgg aagaagctct caaatcaggt attgaagata ttctagttgt
     9481 cactggtaaa tcaaaacgtt ctattgagga ccactttgat tcaaacttcg aattggaata
     9541 taacctcaaa gaaaaaggga aaacagatct tttgaagcta gttgatgaaa caactggcat
     9601 gcgtctgcat tttatccgcc aaactcatcc acgcggtctc ggagatgctg ttttgcaagc
     9661 caaggctttc gtcggaaatg aaccttttgt cgttatgctt ggtgatgact tgatggatat
     9721 cacagacgaa aaggctgttc cacttaccaa acaactcatg gatgactacg agcgtaccca
     9781 cgcgtctact atcgctgtca tgccagtccc tcatgacgaa gtatctgctt acggggttat
     9841 tgctccgcaa ggcgaaggaa aagatggtct ttacagtgtt gaaacctttg ttgaaaaacc
     9901 agctccagag gacgctccta gcgaccttgc tattatcgga cgctacctcc tcacgcctga
     9961 aatttttgag attctcgaaa agcaagctcc aggtgcagga aatgaaattc agctgacaga
    10021 tgcaatcgac accctcaata aaacacaacg tgtatttgct cgtgagttca aaggggctcg
    10081 ttacgatgtc ggagacaagt ttggcttcat gaaaacatcc atcgactacg ccctcaaaca
    10141 tccacaagtc aaagatgatt tgaagaatta cctcatccaa cttggaaaag aattgactga
    10201 gaaggaataa caaaatcatt tatataaaga ttagccacac ataaattaag taaattctct
    10261 acttgaatct acctatttaa taaaaactaa tgaaaacgct atacttgtat ttgttttttc
    10321 attaaaataa gagtagaata aattagtata gtaaaacaaa aaagcaccga atcggtgcgc
    10381 actttttcaa gttgtgtacg gacaaagcct tattttaact ttgctatgtt gtttctaatg
    10441 gttccaaaat aataaataat tttaaatttg acttaactgt tggagtagtc atggttaaat
    10501 taaatcaacc gagccgaaca taagttgttt aattttgtgg aagctattaa taaaaatata
    10561 ataagggaga aagataggtg taattttaat tttaaagtaa ttgcggacac tatcaaagta
    10621 aaagattatg gagaacaaat ttgtagaatt tatcgaaaac aataaaaaag taatcatttc
    10681 atcagttgca gttggtgttg tattgatatt agggtttgga tggtattcat ataaccaaca
    10741 acaagcagaa caacaagcaa aaattgtaca attagaaaaa gatagcaaat cagacaaaga
    10801 acaagttgat aaactatttg aatcatttga tgcatcttca gatgaatcta tttctaaatt
    10861 aaaagaacta tctgaaactt cacttaaaac cgatgcaggt aaagactatc ttaataacaa
    10921 agtcaaagaa tcatctaaag caattgtaga ttttcatttg caaaaaggtt tggcttatga
    10981 tgttaaagat tctgatgaga agtttaaaga taaagcaact cttgaaacaa atgtgaaaga
    11041 aattacaaaa caaattgatt ttatcaaaaa agttgatgaa acttttaaac aagagaattt
    11101 ggaagaaact cttaaatctc taaatgatct tgttgataaa tatcaaaaac aaatcgaact
    11161 tttgaagaaa gaagaagaaa aagctgctga aaaagcaaag gaatcttcta gtcaaagtaa
    11221 ttcttctggt agtgcttcta atgagtctta taatggatct tccaattcaa atgtagatta
    11281 tagttcatct gaacaaacta atggatattc aaataattat ggcggtcaag attattctgg
    11341 ttctggaaat agttcaacaa atggtggatc atcagaacaa tattcatcta gaaattcaaa
    11401 cattggagca aataatggct atacagaata tagaagcatt gatgctagtg gacatgtaag
    11461 agatacttat cataatcgga gtaatggaga tgtgtttgac gaaaatggga attaccttgg
    11521 aaacttgggg ggcggcattg cagaacctcc acaacgctaa taactatttt agagctgtgt
    11581 tgtttcgaat ggttccaaaa cacattaaaa gctactcatt ttttaagtag cttttttctt
    11641 attcaagttt acatattata ctcaatgaaa atcaaattca aaccacgtca gcatcgcc
//
LOCUS       DAVBNO010000057        11448 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVBNO010000057 DAVBNO010000000
VERSION     DAVBNO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.057"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..129
                     /locus_tag="VQ846_001717"
     CDS             <1..129
                     /locus_tag="VQ846_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076257.1"
                     /translation="AMKASQWFKVSDKWYYVNGSGALAVNTTVDGYGVNANGEWVN"
     gene            complement(359..1339)
                     /gene="dusB"
                     /locus_tag="VQ846_001718"
     CDS             complement(359..1339)
                     /gene="dusB"
                     /locus_tag="VQ846_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET3076258.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1326..2198)
                     /gene="hslO"
                     /locus_tag="VQ846_001719"
     CDS             complement(1326..2198)
                     /gene="hslO"
                     /locus_tag="VQ846_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET3076259.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2363..3790
                     /locus_tag="VQ846_001720"
     CDS             2363..3790
                     /locus_tag="VQ846_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET3076260.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            3949..5457
                     /gene="glpK"
                     /locus_tag="VQ846_001721"
     CDS             3949..5457
                     /gene="glpK"
                     /locus_tag="VQ846_001721"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET3076261.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQMDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEIDD"
     gene            5604..7430
                     /gene="glpO"
                     /locus_tag="VQ846_001722"
     CDS             5604..7430
                     /gene="glpO"
                     /locus_tag="VQ846_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET3076262.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELTLSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7500..8204
                     /locus_tag="VQ846_001723"
     CDS             7500..8204
                     /locus_tag="VQ846_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET3076263.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8501..8704
                     /locus_tag="VQ846_001724"
     CDS             8501..8704
                     /locus_tag="VQ846_001724"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076264.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8791..9051)
                     /locus_tag="VQ846_001725"
     CDS             complement(8791..9051)
                     /locus_tag="VQ846_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076265.1"
                     /translation="MTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            complement(9106..9450)
                     /locus_tag="VQ846_001726"
     CDS             complement(9106..9450)
                     /locus_tag="VQ846_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076266.1"
                     /translation="MSKMFKCMCLLVMSVFCMSSISPSLVVFADDSLSANSKVVSGEA
                     QFENGSSVRFGDTQVNILSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEID
                     LNISFRSYDPNV"
     gene            10490..>11448
                     /gene="ltrA"
                     /locus_tag="VQ846_001727"
     CDS             10490..>11448
                     /gene="ltrA"
                     /locus_tag="VQ846_001727"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET3076267.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAASKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIDLTRRIEQLNLS"
BASE COUNT         3391 a         2226 c         2609 g         3222 t
ORIGIN      
        1 gctatgaaag caagccaatg gttcaaagta tcagataaat ggtactatgt caatggctca
       61 ggtgcccttg cagtcaacac aactgtagat ggctatggag tcaatgccaa tggtgaatgg
      121 gtaaactaaa cctaatataa ctagttaata ctgacttcct gtaagaactc tttaaagtat
      181 tccctacaaa taccatatcc tttcagtaga taatataccc ttgtaggaag tttagattaa
      241 aaaataactc tgtaatctct agccggattt atagcgctag agactacgga gtttttttga
      301 tgaggaaaga atggcggcat tcaagaggct ctttaagaga gttacaggtt ttaaactatt
      361 aagccttctc caattgcaag agggcttcaa tctctgctag ggtgctagct tgcgaaatgg
      421 ctccacggag tttggcagcg ccagatgttc cacggagata gtgaggagcg aggccgcgga
      481 attcacgaac tgcgacgttt tctcctttga ggttaatcaa tcgtttcaag tgttcgtagg
      541 cgatcttcat cttgtcttca aatgtcaaat caggtaggat ttctcctgtt tcaaagtaat
      601 ggttgatttg gttgaagagg taaggatttc ccatggcagc tcggccaatc atgactgcgt
      661 cagcaccaac ttcttcgatg cgttgcttgg cttcttggac agtacggata tcaccgttgg
      721 cgatgaatgg aatcttggtt agagcttggg caaccttgta aagggtctca aggtccgcgt
      781 ggccagtata catttgttca cgggtacggc catgcatggc gagggcagaa acacctgcag
      841 cctcagcagc gagggcattt tctactgcaa gagatgggtc cgcccagccg gtacgcattt
      901 tgacagtaag tgggatatca aggacagact ggaccttgtt gatgatagag tagatcttgt
      961 caggatcctt gagccacata gcaccagctt cgttcttcac gattttgttg acagggcagc
     1021 ccatgttgat atcgacgata tcggtcttgg tgttttcttg gatgaattct gctgcgcgtg
     1081 ctaggctgtc ttcatcgcta ccaaaaagtt ggatagagac agggttttcg ccctcatcga
     1141 tatgaagcat gtgcagggtt ttttcgttgt tgtattggat tcccttgtca gagaccattt
     1201 ccattacaac gagtccagct ccgagctctt ttgctatggt acgaaaggct gagttggtca
     1261 cgccagccat aggcgctaaa acggtacgat tgggaatctc aatattgcca atcataaaag
     1321 gtgtattaag atttgtcacg aatgagttcc tccaggtcct tttcatcaaa gttgtaagta
     1381 gtttggcaga attgacaagt gatttctgca ccgtggtctt cctctttcat ttcctgtaag
     1441 tctgagcttg gaaggctggc aagagcgttc ataaagcgtt catggctaca gtcacattgg
     1501 aaacggattt cttcttcaga aagacgcttg taggcttcgt ccccgtagat agccttgagg
     1561 agggcttcga tatggtcgtc gctttcgaga agagtagaga tagctggcat ttcttggatg
     1621 cgtttttcaa agcgagcaat ctcttctttc ttggctcctg gcaagacttg aactaggaaa
     1681 ccacctgcaa ccttgacctt gtcttcctcg tccaaaagga cattgaggcc gaccgctgaa
     1741 ggcgtttgtt ggctttcagt aaggtaaaag gcaaggtctt caccgatttc tccagagatg
     1801 aggggagtta tagagttgta aggatttcca gtaccgtagt ctgtgataac gaggaattga
     1861 ccatttccaa caaaaggtcc gactaggact tcaccagtcg cagtcttttt gatgtcaaca
     1921 ccaggatttt gaacatagcc tttgacgttc cccttggtat cagcgacggt gataatagca
     1981 cctagagagc tagatcccaa caccttaact gtaagtttgg tatttccttt ttcattggct
     2041 gcgagaatct ggctggcgat aagagttcga ccaagcgcta cagttgagct agcttgggtt
     2101 tgatgttttt cttgagcagt gcggacggtt tcagtgctat caaggacaaa agcacgaaag
     2161 gctccgcttt ctgatatagt tttaataatt ttatccatag ctactatttt agcataaaaa
     2221 tgcccaaagg gggagccgtg tgtttactga ttttcaggat aatggaccag gaaatcagca
     2281 tgaaaataaa aagagaaaca gattatttta gcatttgtaa gatttatgct atgcttaagg
     2341 tagaaaatga aagggataac aaatgtattt aggagatttg atggagaaag ccgagtgtgg
     2401 tcaattttca atcctttcct ttctattaca agagtctcag acgaccgtca aggctgtaat
     2461 ggaagaaaca ggattttcaa aagcaaccct aaccaaatat gtcaccctgc tcaatgacaa
     2521 ggctttggat agtggcttag aactgactat tcactcagaa gatgaaaatc tgcgtctgtc
     2581 tatcggtgca gctaccaagg ggagagatat tcggagcttg tttttggaga gtgctgttaa
     2641 ataccagatt ttggtttatc ttctctacca ccaacagttt ttagcccatc agctggctca
     2701 agaattagtg attagcgagg ctacgcttgg tcgtcacttg gctggtttaa atcagatttt
     2761 gtcagaattt gatttatcca tccaaaatgg acgttggcga ggtccagagc atcagattcg
     2821 ctatttctat ttctgtcttt tccgaaaggt ctggtcgagt caggaatggg aaggtcacat
     2881 gcagaaacca gagagaaaac aggagattgc caatttagag gaaatctgcg gtgcaagttt
     2941 gtctgcgggg cagaaattgg acttggttct ctgggctcac atcagtcaac aacgtcttcg
     3001 ggtcaatgct tgtcagtttc aagttataga agagaaaatg cgagggtatt ttgacaatat
     3061 cttctatctt cgtttgctga gaaaggttcc gtcctttttt gctgggcaac atattccact
     3121 aggagttgag gatggtgaga tgatgatatt cttctctttt ctcctatctc atcgcattct
     3181 tcctcttcat actatggagt atattcttgg ttttggaggg cagttggcag atttactgac
     3241 gcaattgatt caagaaatga agaaggagga actattgggg gattatacag aggaccatgt
     3301 cacctatgaa ctcagtcagc tttgtgctca agtctatctc tataagggct atattttaca
     3361 ggatcgctac aagtaccagt tagagaatcg tcatccatat ttactgatgg aacatgattt
     3421 taaagagaca gcagaggaga tttttcatgc tctacctgct tttcaacagg ggacagattt
     3481 agataagaag attctctggg aatggctcca gttaatcgaa tatatggctg aaaacggtgg
     3541 ccagcatatg cggattggtc tggatttgac atctggtttt cttgtctttt caaggatggc
     3601 agccattttg aaacggtatt tggaatacaa tcgttttatt accattgaag cttatgaccc
     3661 tagtcggcat tatgatttgc tggttaccaa taaccctatt cataagaagg aacagacacc
     3721 agtctattat ttaaaaaatg acttggatat ggaagatttg gcagggattc gtcagttatt
     3781 attcacttaa aaggcttggt taatccaggt cttttttgtg aaattcacac aatctcctca
     3841 cattttttta aaaattaaaa aaagttgata aacaagaaag cgctttattt tgtatactag
     3901 taagtgtaaa gaggaaacac ctcaagatct ttatcaggag gacagtacat gtcacaagaa
     3961 aaatacatca tggccattga ccagggaact acaagttctc gtgccatcat tttcaacaaa
     4021 aaaggggaaa aggttagctc gagtcaaaaa gagtttaccc agattttccc tcaagcaggt
     4081 tgggttgagc acaatgccaa tgaaatttgg aactctgttc agtcagttat tgcgggtgct
     4141 ttcatcgaaa gtggtgtcaa gccaaatcaa atcgaggcaa tcgggattac caaccaacgt
     4201 gaaacaacgg ttgtctggga taagaaaaca ggacttccta tctacaatgc tatcgtttgg
     4261 cagtcacgcc agacagcacc tttggctgag caactaaaaa gccaaggtta tgtggaaaaa
     4321 ttccatgaaa agactggttt gattattgat gcttacttct ctgctaccaa ggttcgttgg
     4381 attttggatc atgtagaagg tgctcaagag cgagcagaaa aaggggaatt gctctttggt
     4441 actatcgata cttggttggt ttggaaattg actgacggtg cggctcacgt gactgactac
     4501 tcaaatgcag ctcgtaccat gctttataac attaaagaac tcaaatggga tgatgagatt
     4561 ttggaaatcc ttaacattcc gaaggctata cttccagaag ttcgttctaa ctccgaaatc
     4621 tacggcaaga cagctccatt ccatttctac ggtggagagg tgccaatctc aggtatggct
     4681 ggggaccaac aagcagccct ctttggacag ttggcttttg agccaggtat ggttaagaat
     4741 acttatggaa caggctcttt catcatcatg aatactgggg aagagatgca gttgtctgaa
     4801 aacaacctct tgacaaccat tggttacgga atcaacggta aggtttatta tgccttggaa
     4861 ggttctatct tcatcgcagg aagtgctatt cagtggcttc gtgacggtct tcgcatggtt
     4921 gaaaattcac cagaatctga aaaatacgct cgtgattctc acaacaacga tgaagtttat
     4981 gtcgttccag cctttacagg tctaggcgct ccatactgga accaaaatgc tcgtggttcc
     5041 gtctttggtt tgactcgtgg aacaagcaaa gaagacttta tcaaggcgac tttgcaatct
     5101 attgcttatc aagtgcgtga catcatcgac accatgcaaa tggatactca gaccgccatt
     5161 caagtactga aggtggatgg tggtgcagcc atgaacaact tcctcatgca gttccaggcg
     5221 gatattttag gcattgacat tgcacgtgct aaaaacttgg aaacaacagc tctaggagcg
     5281 gccttcctag caggtttgtc agtagggtac tggaaagact tggacgagtt gaaactcttg
     5341 aacgagacag gagaactctt tgagccatct atgaacgaat ctcgcaagga acaactctac
     5401 aagggctgga agaaggctgt gaaagcaact caagtctttg cggaaatcga tgactaatac
     5461 tcaatgaaaa tcaaagagca aactaggaag ctagccgcaa gctgtacttg agtacggtaa
     5521 ggcgacgctg acgtggtttg aagagatttt cgaagagtat aatactggca gaataaagcg
     5581 atttatttag aaagtgtgta aatatggaat tttcaaagaa aacacgtgaa ttgtcaatta
     5641 aaaaaatgca ggaacgcacc ctggacctct tgattatcgg tggaggaatc acaggagctg
     5701 gtgtagcctt gcaggcggca gctagcggtc ttgagactgg tttgattgaa atgcaagact
     5761 ttgcagaagg aacatctagt cgttcaacaa aattggttca cggaggtctt cgctacctca
     5821 aacaattcga cgtagaggtg gtctcagata cggtttctga acgtgcagtg gttcaacaaa
     5881 tcgctccaca cattccaaaa ccagatccaa tgctcttacc agtttacgat gaagatggag
     5941 caacctttag cctcttccgt cttaaagtag ccatggactt gtacgacctt ttagcaggtg
     6001 tgagcaacac accaactgcg aacaaggttt tgagcaagga tcaagtcttg gaacgccagc
     6061 caaacttgaa gaaggaaggc ttggtaggag gtggagtgta tcttgacttc cgtaacaacg
     6121 atgcgcgtct cgtgattgaa aacatcaaac gtgccaacca agacggtgcc ctcattgcca
     6181 accacgtgaa ggcagaaggc ttcctctttg acgaaagtgg caagattaca ggtgttgtag
     6241 ctcgtgatct cttgacagac caagtatttg aaatcaaggc ccgtctggtt attaatacaa
     6301 caggtccttg gagtgataaa gtacgtaatt tgtctaataa gggaacgcaa ttctcacaaa
     6361 tgcgcccaac taagggagtt cacttggtag tggattcaag caaaatcaag gtttcacagc
     6421 cagtttactt tgacacaggt ttgggtgacg gtcgtatggt ctttgttctc ccacgtgaaa
     6481 acaagactta ctttggtaca actgatacag actacacagg tgatttggag catccaaaag
     6541 taactcaaga agatgtagat tatctacttg gcattgtcaa caaccgcttt ccagaatcca
     6601 acatcaccat tgatgatatc gaaagcagct gggcaggtct tcgtccattg attgcaggga
     6661 acagtgcctc tgactataat ggtggaaata acggtaccat cagtgatgaa agctttgaca
     6721 acttgattgc gactgttgaa tcttatctct ccaaagaaaa aacacgtgaa gatgttgagt
     6781 ctgctgtcag caagcttgaa agtagcacat ctgagaaata tttggatcca tctgcagttt
     6841 ctcgtgggtc tagcttggac cgtgatgaca atggtctctt gactcttgct ggtggtaaaa
     6901 tcacagacta ccgtaagatg gctgaaggag ctatggagcg cgtggttgac atcctcaaag
     6961 cagaatttga ccgtagcttt aaattgatca attctaaaac ttaccctgtt tcaggtggag
     7021 aattgaaccc agcaaatgtg gattcagaaa tcgaagcctt tgcgcaactt ggagtttcac
     7081 gtggtttgga tagcaaggaa gctcattacc tagcaaatct ttacggttca aatgcaccga
     7141 aggtctttgc acttgctcac agcttggaac aagcgccagg tcttagtttg gcagacacct
     7201 tgtcccttca ctatgcaatg cgcaatgagt tgactcttag cccagttgac ttccttcttc
     7261 gtcgtaccaa tcacatgctc tttatgcgtg atagcttgga tagtatcgtt gagccagttt
     7321 tggatgaaat gggacgattc tatgactgga cagaagaaga aaaagcaact taccgtgctg
     7381 atgtcgaagc agctctcgct aacaacgatt tagcagaatt aaaaaattaa gaaaaaataa
     7441 aagaggtgga gggcagcatt ccttgtcgcc cgtcccttct ttttaatgga gacagaaaga
     7501 tgatgaatga attatttgga gaatttctag ggactttaat tctgattctt ctaggaaatg
     7561 gtgttgttgc aggtgtggtt cttcctaaaa ccaagagcaa tagctcaggt tggattgtga
     7621 ttactatggg ttgggggatt gcagttgcgg ttgcagtctt tgtatctggc aagctcagtc
     7681 cagctcattt aaacccagct gtgaccatcg gtgtggcctt aaaaggtggt ttgccttggg
     7741 cttccgtttt gccttatatc ttagcccagt tcgcaggggc catgctgggt cagattttgg
     7801 tttggttgca attcaaacct cactatgagg cagaagaaaa tgcaggcaat atcctggcaa
     7861 ccttcagtac tggaccagcc atcaaggata ctgtatcaaa cttgattagc gaaatccttg
     7921 gaacctttgt tttggtgttg acaatctttg ctttgggtct ttacgatttt caggcaggta
     7981 tcggaacctt tgcagtggga actttgattg tcggtatcgg tctatcacta ggtgggacaa
     8041 caggttatgc cttgaaccca gctcgtgacc ttggacctcg tatcatgcac agcatcttgc
     8101 caattccaaa caagggagac ggagactggt cttacgcttg gattcctgtt gtaggccctg
     8161 ttatcggagc agccttggca gtgcttgtat tctcactttt ctagtttata ctcttcgaaa
     8221 atcaaattca aaccacgtca gcgtcgcctt accgtactca agtacagctt gcggctagct
     8281 tcctagtttg ctctttgatt ttcattgagt attagaaaac aattatgttg atagagcttg
     8341 ggcaagagcc caatttcagc aaaaaatgaa gtaaatcttc tcataataaa acgcatcata
     8401 tcaagcacga aaattccacg aggtcaacta cagtcagaaa gctgaacaac aagccaaaac
     8461 gcccaaaaaa ggcggcaaaa agcaagcacc tgcaagcaac gtgccgaaat ggtcaaatcc
     8521 tgattatgtc aacgaattag acccaaaaat cgttgatatg ctagtagaat ttcacaagtc
     8581 acaaggcact ttggaaactc ccgaggcgca agcagaaatc gcccaaaaac gtgaagaaat
     8641 cgagcaaagg agagctgagc ttgaggataa aaaacaagag cttttgaacc gcttgaacaa
     8701 atagagtttc gcaagtatta tgcttacaaa ttacttgagc aattaactaa aatataaacc
     8761 ctgcctttat atctaggcag ggtttatatt ttagaaattc acgtaggttg ttacggtttt
     8821 tacataccca gtatagtttg agtttctata gtattcagtg ataaacttcc attttctttg
     8881 agcaacatgg atataagtac ttgttatgta gtatggatat gggctttgtg aatccaagta
     8941 agactgataa gcttgtatac caaaatatgc tccaccaatt attgcacccc atggaccccc
     9001 caataaagca cctatcctac caatcatata actgattcca gcaccagtca tgaagttagc
     9061 gaatgtgtta gcttgtttat tcccatgtat tgtgttgacg taatttcaaa cattaggatc
     9121 gtatgatcta aaagatatat ttaggtcgat ttcattcttt tgataagcca tataaaatgc
     9181 cccattgata tagacgccgt cagcacgtcg ttcaatagtg tctacacttc catctggatt
     9241 gacaacctca agaacttcat cgcttaaaat atttacttgc gtatctccga accgcactga
     9301 tgagccattc tcaaactgag cctcaccaga tacaacttta gagtttgccg ataagctatc
     9361 atcagcaaaa acaacaagcg acggggaaat gctagacata cagaaaacag acataactag
     9421 caaacacatg catttaaaca tcttagacat aacggaaact cttttgtatt tttgattttt
     9481 ttcaactttt attatacaat aaaaccaaat aaaaagaaag cggtaacaat atgcttaatg
     9541 cgaaaatttt ttatatattt ttatgtttga tcgttatcga aactacaggc ttgttgttgt
     9601 tgaaaagagg tctcgaaatg ggttatttag acacagaagc tattatcctc gcagtttttt
     9661 catttgcttt ttacaaccta tgttcattcg cttgggtctg ctctacaata aaaaacaata
     9721 aaaaataaat agacgtattt tcaaaaaaaa aaaaaaatgc atatttatct tagcaaaatg
     9781 acgatttaaa tcgtcgtttt tttgtagtac gacgggcatg tcgtatatct gaggtgtaag
     9841 tcctcggtgg gcacctgcta ccggtgaacc caatagcgat tcccaagcct gactatcgtg
     9901 aggtagcagg gagaggaagg gatagcgaaa tcgtggctct acgaacagga acgtgatagt
     9961 aaggcgtata tagcggataa ggaggcttca aactctaaag tccaaaaagg tagtcgtaac
    10021 ctatatgtgt aaatcacgag agtaattgaa ttcggactaa ggtttgtgtg aaaaagataa
    10081 atctttctag agtctaaaga ctctgcgtca gatttcctat tttcactgta accttttaac
    10141 gtcctcatat cttgtataaa cgaggaaaga tgtacgactt atcccgtgag gtttcatgag
    10201 cgctgaaagc gtagtaacaa cgaatcatga gaagtcagcc gagcccatag tagtgaggaa
    10261 acttctgtag tggaagtgga gcgacggggt gaatactcaa acagtctggg gagagactgt
    10321 ttgaggtctg tcgctagaaa gagaaaacga cagatcgaag taatcctact tcacttgtgt
    10381 ctgtaaaatg agtggtctga tagaactgga ctttgacacg taatgactag atgaaggttc
    10441 accaatataa gatgtccctc aggcaccaaa acaagaaagg aataggcaca tgtcaaaact
    10501 gctagataag atattatcac gcgaaaatat gctggaagcc tacaatcaag taaaatccaa
    10561 taaaggctca gctgggattg atggaatgac tatcgaagag atggataatt atctcagaca
    10621 aaactggcgc ttgactaagg aactgataaa acagagaaaa tataagcctc aaccagttct
    10681 taaagttgag atacctaaac cagacggagg catccgtcaa ctaggaattc caacagttat
    10741 ggatagaatg attcaacagg ccattgtcca agtcatgagc cccatttgtg aaccccattt
    10801 ctcagatacg agttatggtt tcagaccaaa taggtcatgt gaaaaagcca tcatgaagct
    10861 cttagaatac ttaaatgacg gctatgagtg gatagtggat atagacctag agaaattttt
    10921 cgatacagtt cctcaagata gattgatgtc cttagtacat aacattatcg aagacggaga
    10981 tacggaatcc ttgattcgta agtatcttca ttcaggtgtt atcattaatg gtcaacgtta
    11041 taaaacacta gttggtacac cacagggagg aaatttatct cctctcttat ccaatatcat
    11101 gcttaatgaa ttggacaagg aattagaaaa gaggggactt cgatttgtgc gctacgcaga
    11161 tgattgtgtg attacggtcg gaagcgaggc agcctctaag cgtgtgatgt attcagtcag
    11221 tcgttttatt gagaaacggc taggtttgaa agtaaacatg accaagacta agattaccag
    11281 accaagagag ttgaaatatc taggttttgg gttctggaaa tcatcagatg gttggaaaag
    11341 ccgtccacat caagatagtg ttcggagatt taagtttaaa ttgaagaaac taacacagag
    11401 gaaatggagt atagacctaa caagacgtat tgagcaactg aatttgtc
//
LOCUS       DAVBNO010000058        11410 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVBNO010000058 DAVBNO010000000
VERSION     DAVBNO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.058"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(103..2100)
                     /gene="metG"
                     /locus_tag="VQ846_001728"
     CDS             complement(103..2100)
                     /gene="metG"
                     /locus_tag="VQ846_001728"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET3076268.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEITYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2224..3483)
                     /locus_tag="VQ846_001729"
     CDS             complement(2224..3483)
                     /locus_tag="VQ846_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3076269.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3485..4186)
                     /locus_tag="VQ846_001730"
     CDS             complement(3485..4186)
                     /locus_tag="VQ846_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3076270.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4170..5369)
                     /locus_tag="VQ846_001731"
     CDS             complement(4170..5369)
                     /locus_tag="VQ846_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET3076271.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5634..6980
                     /gene="gor"
                     /locus_tag="VQ846_001732"
     CDS             5634..6980
                     /gene="gor"
                     /locus_tag="VQ846_001732"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET3076272.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDSSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RINLPLHTHKVPAKLEKTAKGITIHFEDGTSHTASQVIWATGRRPNVKSLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFTSMYSACTCNRQETRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMRATKADFDATVAIHPTSSEEFVTMR"
     gene            7176..7712
                     /locus_tag="VQ846_001733"
     CDS             7176..7712
                     /locus_tag="VQ846_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET3076273.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            7763..7849
                     /locus_tag="VQ846_001734"
                     /pseudo
     CDS             7763..7849
                     /locus_tag="VQ846_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7918..8133)
                     /locus_tag="VQ846_001735"
     CDS             complement(7918..8133)
                     /locus_tag="VQ846_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET3076274.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            8231..8551
                     /locus_tag="VQ846_001736"
     CDS             8231..8551
                     /locus_tag="VQ846_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076275.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(8582..8920)
                     /locus_tag="VQ846_001737"
     CDS             complement(8582..8920)
                     /locus_tag="VQ846_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076276.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(8932..9651)
                     /gene="trmD"
                     /locus_tag="VQ846_001738"
     CDS             complement(8932..9651)
                     /gene="trmD"
                     /locus_tag="VQ846_001738"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET3076277.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQVYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(9641..10159)
                     /gene="rimM"
                     /locus_tag="VQ846_001739"
     CDS             complement(9641..10159)
                     /gene="rimM"
                     /locus_tag="VQ846_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET3076278.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(10190..10978)
                     /locus_tag="VQ846_001740"
     CDS             complement(10190..10978)
                     /locus_tag="VQ846_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET3076279.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGRNHHYLQWSESEKIAAIIRELSE"
     gene            complement(11082..11325)
                     /locus_tag="VQ846_001741"
                     /pseudo
     CDS             complement(11082..11325)
                     /locus_tag="VQ846_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
BASE COUNT         3274 a         2508 c         2077 g         3551 t
ORIGIN      
        1 cagtgctttg atcaacccgc ggctagcttc ctagtttgct ctttgatttt cattgagtat
       61 aaataaaaaa acagaactag tctgaactag tcctgtctac ttttacccaa tcacacttcc
      121 atttggtaca gctggatcaa ctgtgagaag ggttaatttg ccatcatgtt cagctgatag
      181 aatcataccc tggctgacat attttttcat cattttacgt ggtttgaggt tagcaacgat
      241 ttgaactttc ttgccgacca attcttgttc atttggatag tattttgcaa ttcctgaaag
      301 aatctgacga tcttctccat caccagcatc caagcggaat tgaagcaact tatctgaacc
      361 ttctacttta gacacttctt tgacttctgc gacacggatt tcaaccttgt caaagtcttc
      421 aaacttgatt tcatccttgt ttagtttgag ctcaacttcg tccggattcc attctttttc
      481 gactgctggt ttattgcctt ccatttgttc cttgatatag gtgatttctt cttccatatt
      541 tagacgtgga aagataggtg ttcctttggc aactacagtc acatctgctg ggaagtcagc
      601 caaactcaag ttttcaagac tagaaacttc ttccaaacca agttgagtca aaactgcacg
      661 actagtttcc atcataaatg gttcaatcaa gtgagcaact acacgaatgc tggctgccaa
      721 gtggctcatg acacttgcca attggtcacg aagagcttca tccttggcca agacccatgg
      781 tgcggtctca tcgatgtatt tattggtacg agagatcaga gtccagactg cttcaagcgc
      841 acgtggatag tcaactgctt ccatgtgtgt atggaagtct gcgattgatt gttctgcaac
      901 ctcagcaaga acatgatcaa attcagtcac accttctaca taggcaggga tttgtccatc
      961 aaagtactta ttaatcatgg aaaccgtacg gttaaggagg ttcccaaggt cattagccaa
     1021 ttcatagttg atacggccga catagtcttc aggagtaaag gttccgtctg aaccaactgg
     1081 aaggttacgc atgaggtagt aacgaagtgg atctagtcca taacgctcta ccaacatttc
     1141 agggtaaacg acattccctt ttgacttaga catttttccg tctttcatga caaaccaacc
     1201 atgggcaatc aaacgatcag gtaatttaac atccaacatc ataagaagga ttggccagta
     1261 gatagagtgg aagcgaagga tatcttttcc taccatatgg aagactgttc cattccagaa
     1321 cttgtcaaag ttaccatgtt cgtcttgagc gtagccaaga gctgtcgcat agttaagaag
     1381 ggcatcaatc caaacgtaga caacgtgttt tggatttgat gggacaggca ctccccatgt
     1441 aaaggttgta cgagataccg ccaaatcttc caaacctggc tcgatgaagt tgcgtagcat
     1501 ttcattaagg cgaccatctg gcgtgataaa ttcaggatga gctttgaaaa attcgaccaa
     1561 acggtcttgg tatttgctaa ggcgaagaaa gtatgattct tcagaaaccc attcaacctc
     1621 atggcctgat ggagcaatac caccagtcac atttccagct tcatcacgga aaacttctgc
     1681 cagctggctt tctgtaaaga attcttcgtc tgatactgaa taccaaccag agtattcacc
     1741 caagtagata tcatcttgag caagtaagcg ttcaaagact tgtgcgacaa ctttttcatg
     1801 gtagtcatca gttgtacgga taaatttatc gtatgagata tctagtaatt gccagagttc
     1861 tttaactcca accgccattc catcaacata ggcttgaggt gtaataccag cttcttccgc
     1921 tttctgctgg attttctgac catgttcatc aagacctgtc agataaaata catcgtagcc
     1981 catcaggcgt ttgtaacgtg ctaggacatc acatgcgata gttgtgtagg cagaaccgat
     2041 atgaagtttc ccagatggat agtaaatcgg cgttgtaata taaaaatttt tttcagacat
     2101 aatttttcct ttccaggcaa atgaaacctg tttttctaac acttcattat atcacatttt
     2161 taatgaattt cgatagggaa atccatacaa aaacaagata gacgagtgtc catcttgttg
     2221 atctcattca taacgaaggg cttcaattgg atcaagtttc gatgccttgt tggctggcaa
     2281 gactccaaaa atcataccaa cactagccga aactgcaaga ctaaataggg caactgggat
     2341 tgatactcca acttctatac cttctattaa accttgcagt aacaaacctg ctaaggcagt
     2401 taaaccactt gcaattgtca agccaattaa gccacctaac aaggtcaaaa tcatggattc
     2461 aatcaaaaac tgaattaaaa tattggcacg tgttgcgccc aaagccttac gaagaccaat
     2521 ctcacgagtg cgctctgtca ccgaaaccag catgatgttc atgacaccag ttcctccaac
     2581 aaagagagaa atccctgcga tggaactaat aatcgtcgtc ataaaactaa acgattgttg
     2641 aatttctgca aatacaacgg actcatctgc cacctggtat tctccctgtt gtaagcctgc
     2701 aagctctgtc atttttcgtg ccagttctgg acccagagtt ggggttaaac tggtatcatt
     2761 cactcgaaag acaatactag ctatttcatc tatattaaaa ttcgcagcaa gggagatatt
     2821 ggtagtaata ggcaagccac caaacccata tatttttgac cttttagcct ccggactagt
     2881 ataaacccca atgacccggt aactaaatcc attgacttct acaaccttgt taatagcctc
     2941 ttgaggagat tcaaataaac taatggacaa ttcctcatct agcaaaataa cacttgcaaa
     3001 ctctttgaaa tcttgctctc tcagactacg acctgcaata atttcattct taacagcgtc
     3061 catgtaagtt ctgtttccac ctgtcaaatt agcattctca acctttttat cttgataggt
     3121 caagatggca ttcgttgaat tggttacata gtaactatcc actcccttca gtttagctgc
     3181 ctcttgaacc caggattctt gcggttttgg cggttcaaca ggaacttcct cttcctttcc
     3241 agaaaccgta aaagctgatt gtttctgagt aaaagaccca tctttacttt ttttaggaga
     3301 gaaaaagacg ctaatatttt tctgagattt agtcatatct ttattgactt gacgagatag
     3361 ggaatcaccc aaagccataa tcacaacaac tgatgaaaca ccgataataa tcccaatcat
     3421 agtaagcaaa gaacgcatct tgtgagccat gatagatgaa aaggcaaatt tcagattctg
     3481 catcttagtt ttcctccttt cctaactgag cactgtcaga cgaaatgacc ccatcccgaa
     3541 tgacaatctg acgtttggca taggcagcaa tctcaggctc atgcgttacc atgataatgg
     3601 tttttccttc tttattcaaa tcaaccaata attgcataat ttggttacct gttttggtat
     3661 ccaaggctcc tgtcggttca tccgctagga taatagaagg attgtttacc aaggcacgcg
     3721 caatggctac acgttgcttt tgaccaccag ataattctga aggtaaatgg tgactacgtt
     3781 ctgtcaattc aaccttgtct aaatattcct cagccaactt gcgacgtttt gaagacgaaa
     3841 ctcctgcgta aatcaagggc aattctacat tttgcagagc attgagcttc gatagaagaa
     3901 agaactgctg aaagacaaaa ccgatttgtt ggttacgaac cttagctagt tgtttttcac
     3961 caagcccagc cacttcttga ccttcaagat aatattctcc actggttggt gtatccaaca
     4021 tgccaatcgt attcatcaga gtggacttac cagaaccaga tggccccatg atggctacaa
     4081 attcaccctc attcacttct agattgatat ttttgagaac ctgcagttct tggtcaccat
     4141 tacggtagct tctgaagata ttttttagac taattagttg cttcatcagc cttcacctct
     4201 tttccttctt ccaaggaaga tgttggatta ctgatgacct tagcaccatt tgtcaaacca
     4261 gaagtgattt cttgattttc tgcgtcagca tttcccaatg aaacctcaac ttttttagcc
     4321 ttttgttgtt catccacaat ccagacataa tttttactat catccattac tagactgcta
     4381 acaggaacaa gaatagcctt agttttgctt ttaacctcaa tgttgacaga aaaaccttgt
     4441 ttcaaatcac caacctcgcc tgtcacatca atagtataag ggtatttaga acctgtatta
     4501 ttcccggctg ctggactagc tgcttcacca ttgtttttag gatagtcaga aatatagctt
     4561 aatttcccag tccatttttt atcaggatac actttagaag taaagcttac ttcttgacct
     4621 acagaaaggt tggctagatt gtactcagac aattctccct tgacttgtaa attttcattg
     4681 ctgacaatat gaaccataac ttgactcgcc cctgttggag atttagaaac attgctattg
     4741 acttcgacta cagttccctc tagggtactg agaacagttg ttgcatccaa ttgactttga
     4801 gccttgctta attgcgctgc agcatctgca cgcgcatcac gggcatcacc caattgagca
     4861 tcaatagaag caacagaatt tccagccact ggagttgggc tttgcaccgt tgcatcttct
     4921 cctcctactg gcgctggtaa ctgtggagcc tgagctgaag cggcttcatt tcgtgcttga
     4981 ttgagttcat tgatatgacg atctgcctta gctactgctc gactagctga atcataggcc
     5041 gcctgcgctt ctgaactact gtacttgact aaagcctgcc cttcgctgac cttatcgccc
     5101 acagaaacaa ggatttcatc taaatcaccc ttactagcat caaaataaac atattgttca
     5161 ttttttgctg ttactgtccc tgacaataaa acagaggatg ccacgcttcc ttccttggca
     5221 acaacaagat gagtaggctc atcttttaca gcagtctgag aaggttgtct aaaaagtaaa
     5281 atccccccgg cacccaatac aactacactc gcagcaccga ttgctgcata cagttgccac
     5341 tttttagctt taccattctt tttcttcata atgaaactcc ttttcttttt tacaatactt
     5401 tgctattata ccaaatttcc ctccagcaaa caatacagtt caggattaag caatcgttcg
     5461 gaatttttct tttctattgt actagatata taatacacta tcttgactta tttggcaagc
     5521 ttttttgcaa ttttttgtgc gtagcagatt tttgcaatca aatcaaaaaa agaataaaat
     5581 ataactatca aaaaagacgc tctactattt aaaagattac aaaggagttt tcaatgagag
     5641 aatatgatat cattgctatc ggtggaggta gcggaggaat cgctaccatg aaccgtgctg
     5701 gtgaacatgg agccaaagca gccgttattg aggaaaagaa attaggtgga acctgtgtca
     5761 acgtcggttg tgttcctaaa aaaatcatgt ggtacggggc gcaaatcgct gagactttcc
     5821 atcaatttgg agaagactac ggctttaaga ctactgatct taactttgac tttgcaaccc
     5881 tacgtcgcaa tcgtgaagcc tacattgatc gcgctcgttc ttcttatgat agtagtttta
     5941 aacgcaacgg tgtagacttg attgaaggtc atgctgaatt tgtagattct catactgtaa
     6001 gcgtaaatgg tgaactgatt cgtgctaaac atatcgtgat tgctacaggt gcccatccaa
     6061 gtattcctaa tattcctggt gctgagctag gtggctcttc tgatgatgta tttgcctggg
     6121 aagaacttcc agagtcagtt gccattctag gcgctggtta tatcgccgtt gaattagctg
     6181 gcgtactcca cacttttggt gtcaagacag atctctttgt tcgccgcgat cgtcctttac
     6241 gtggttttga ttcctacatc gttgaaggtt tggtcaagga aatggaaaga ataaacttgc
     6301 cacttcatac tcacaaagtc cctgccaagt tagaaaaaac tgctaaaggc attaccattc
     6361 atttcgagga tggtaccagt cacacagcta gccaagttat ctgggctaca ggtcgccgtc
     6421 caaacgttaa gagcttgcaa cttgaaaaag ctggagtgac tctgaatgaa cgtggcttta
     6481 tccaagtaga tgaataccaa aatactgttg ttgagggaat ctatgctcta ggtgatgtaa
     6541 caggtgaaaa agaactgact ccagttgcaa tcaaggctgg gcgtacccta tcagaacgtc
     6601 tctttaacgg aaaaacaact gcaaaaatgg attactcaac tattccaact gttgtctttt
     6661 cacaccctgc tatcggaact gttggtttga cagaagaaca agctattaaa gaatacggtc
     6721 aagaccaaat caaggtttac aaatcaagct ttacttctat gtactctgct tgcacttgca
     6781 accgtcaaga aactcgtttc aaattgatca cagctggttc agaagaaaaa gttgtcggac
     6841 ttcacggaat tggctacggc gttgatgaaa tgattcaagg atttgctgtt gctatcaaaa
     6901 tgagagcaac caaggctgac tttgacgcaa ctgtagcgat tcacccaact tcatctgaag
     6961 aatttgtaac catgcgttaa tcgaaacaaa agaagctgat ataaatatgt ctgcttcttt
     7021 tttaatgatt ttcgaatcaa caaaaaatta tccaacttga ctagtcacga aaatcggctg
     7081 ttacagtttt tgttaccttg tttttaaaaa atggttgaca tcgactccta tatgagtata
     7141 atagttacta tacattagaa aagaggttaa ctattttgaa aaaagctcac gtttatgcta
     7201 tccctgctat tggggctgct ctcattgctg ttttggcaca aatcagtctt ccaattggac
     7261 ctgttccctt cactctgcaa aactttgcaa tcggcttgat tgctactgtc tttagaccga
     7321 gagaggctgt actttctgct ggactctatc ttcttctagg tgctatcggt cttcctgtct
     7381 ttgcaggagg tggagctggt tttcaggctt tagttggccc tactgcaggc tatctttggt
     7441 tttatctcgt ttactctgga cttacttcct ctctaaccaa cagcaagagt ggtgttgtta
     7501 agatttttct tgcaaacctc ttgggtgatg cccttgtctt tgtcggcggg attctcagct
     7561 tgcatttcct agctggaatg gcatttgaaa aagctcttgc tgtgggggtt cttcccttta
     7621 tcattccaga ccttggcaaa cttctggcta ttagttttat tagccgtccc ctacttcaac
     7681 gccttaaaaa tcaggcttac tttactaact aaaaaaggat atcgagttat catgactcaa
     7741 tatccttttc ttttattttg aaaacttata ctcaatgaaa atcaaagagc aaactagaaa
     7801 gctagccgca ggctgctcaa aacactgttt tgaggttgtg gatgaaactg acgaagtcag
     7861 taaccataca tacggcaagg caaagctgac gcggtttgaa gagattttcg aagagtatta
     7921 gttgccttta aaggcatcca ccatcgtttg aaattcttca tttgagagag taatcccttt
     7981 gcccatttta gtatggtctg gactccaagc acgaatatca aactttgcag gggcaccatt
     8041 aaagctcaca cggttaattt ccttggtcca acctttttcg ttttcagaaa gagtcaacaa
     8101 gtgctcttcg atttcaaatg taaattctgc cattttcttc tcctttttta gtttcattag
     8161 tttattcgta aaatcttgta gattttagga aaattttata taatattgat ataaaagaag
     8221 ggaggccaat atgagacata aattccagca agttctaaat aaaatacatg attttttaaa
     8281 tggatatgac caacctgacc agactgaaac caactccctt acagccacta ttgaagaggc
     8341 tatccagaaa caaaccgctg ttcaccttat cttgtctgag acaagtttta caggtgatat
     8401 catcaaatat gatcagcaag gccagcaaat tatcgtgaaa aattttgcca aaaatgtgag
     8461 ccggattatc cgtataagcg atattcaacg cctgcgattt gtcccctcaa ctgtccaaac
     8521 agcccaaaaa aatagattta agaaagagtg agatgtagtt gcttcatccc actctttttt
     8581 cttagcgaat ttgttcaaaa tgtaaatgaa ctgcaatatg atctccataa ccacttcttt
     8641 ccaagtcacg ttgtaaacga taggaaatgt agtgttctgc aatggtaatg taacctgcgc
     8701 ccaataaacg atgttcaacc atagattgaa tcatactgat agtcgcacgt tccaccttgg
     8761 cttcttgtaa atccaaaact accttcttag tgacttgagc aagattttga cgcaaatcat
     8821 ctgtcaaaac ataaacagtt tgggctgcct tcaagatggc ttggtaaatc ttatctggat
     8881 taaattcagc aatttcgcca ttacgtttga ttacttgcat aggtttctcc tttattcttt
     8941 gttttctttg atttctgcca gcattttttc ttcttctact gtcagttgat aatgttcaag
     9001 taaatccggt ctgcgctcgt aggttttctt taaactctcg tacaatcgcc actgacgaat
     9061 cttttcatgg tggccactca tcaatacatc tggcacgacc atgcctcgat aatcataggg
     9121 acgtgtgtac tgaggatatt ctaaaagacc tgaagaaaaa ctatcatctt ggtggctaga
     9181 ctccttgcca atcacttctg gaatcaggcg aactgtagca tcaatcatgg tcatagctgc
     9241 caattctcca ccagtgagga catagtcgcc tagggaaatc tcatctgtta ccaaggtctt
     9301 aatgcgctca tcataaccct catagtgccc acagataaag attagctctt cctcttgagc
     9361 caaatcttca gcataaacct gatcaaactg ctttccagca ggatcgagga gaataacgcg
     9421 cggatttttc ttttcaatag catcaaagga atcgaaaata ggttgtgctc tgagcaacat
     9481 gccctgaccg cctccgtagg gctcatcatc tacatgacgg gccttttcag cattttctcg
     9541 aaaattatga tactggatat ccaagagccc tttttctcga gcctttccaa cgattgagtg
     9601 ctccagtgga gaaaacatct ctggaaagag ggttaaaata tcaatcttca tcgtctaacc
     9661 cttctaagat ttccacatcg acccgtttat ttggaatatc aacattgaga accactggtg
     9721 ggatataagg taaaagcaaa tcacgtttgc cttttcgttt gaccacccag acatcattag
     9781 cacctggttg caggatttcc ttgatggttc caaccaagct atcaccctca tagacttcca
     9841 aaccgataat ctcgtgatag taaaattcac catcgtctag gtcattcaaa tcttcctcag
     9901 cgaccttgag actgtatccc ttgtactttt cgatagtatt gatatggtac atatctttga
     9961 atttaataat gtcaaagttc ttctgtttac ggtggctagc gatggtcact gtttggacaa
    10021 actgatcttt ttcatcaaac aaagccagct cagctccttt tttaaaccgt tcttctgcaa
    10081 aatccgtcac agacaagact cgcatctcac cctgtaatcc ctgcgtatta acgattttcc
    10141 caacattaaa gtagttcatc ttgtctcctg taatctcctt ttttccatct tattctgaca
    10201 attctcgaat aatagccgca attttttccg attctgacca ttgtaaataa tggtgattcc
    10261 ttcctaaaat gagtttagta ttggaagtcc aatattctga ttctctgtac tctttttctc
    10321 tataagtctg acaaaaaata aatacaggga catgagattc tatcgacaaa ttctcaaaat
    10381 cttcctcagt aatctctcca gatatctgaa attctggatc ttgattttcc aactctaagc
    10441 ctttttcttg cattaattcc cagatttttt tattcgtttc aggactaaat gttgcttgag
    10501 ttaagttctt aaaataaagt tcaggaccac actcgtcaat cagcctcatc tgctcttcca
    10561 tttctggata aggattttct gaaaaatcag caaacatgac ttttttagtt gtcggttcaa
    10621 ttgctactaa agtctgacgc ttaattggtt tctcgagtaa tttgcaagct aaaattccac
    10681 tccaactatg tgcacaaagt atatattcag aaattcctaa ttcttcaagt acttcataaa
    10741 ccgcatctgc aagattatct agattttttc cagcttggtc atgaatcgga ctcctacctg
    10801 tgttcggaaa atcaattgtc aaataaccaa ttgtaggagg aggtttttca agtataagtg
    10861 aaaaattttc ataacttggt agcaaacctg ctccgtttag acaaactagc actttctttt
    10921 gcttttgata agtaacagag aggctaccaa tttctgtaga tacttcaaac ctcttcataa
    10981 agaaatccac tcattctata taatgaatta ttaaaaatcc ttatccttta ttttatcacg
    11041 ttccaaagat tttctcaagt tggaggaagg ggacaacatc tctactttcc cttcaagaat
    11101 ccttccaaat catgtttatg ttggtaatta acggctgcgg ttttgtcttt ctcaaagatg
    11161 gttctagtta ggtcaatatg cttgatagca gcaatagcga cggtgtagtg aatgatatca
    11221 gccagttcta tggcaggttc ctcgttcgaa tcctatccct tcttttcgac cagagcgcct
    11281 attcaaaacc tcggctactt ctccgacttc ctccactaac ttcataaaga gaccttcatc
    11341 agtccgagac tgctgttaat gttcgagtaa gtagtcttgg aattgcctaa acgttcaatc
    11401 ttttatagta
//
LOCUS       DAVBNO010000059        11007 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVBNO010000059 DAVBNO010000000
VERSION     DAVBNO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.059"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(128..1759)
                     /locus_tag="VQ846_001742"
     CDS             complement(128..1759)
                     /locus_tag="VQ846_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET3076280.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2052..6992
                     /locus_tag="VQ846_001743"
     CDS             2052..6992
                     /locus_tag="VQ846_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET3076281.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFVGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     AGEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVQAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQQVNANLSVTVTGQDEASPKTILGIEVSQKP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLRYQGH
                     EVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHEISTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLDAKPNHPSGS
                     ALAPLLEKNKVLVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7082..8278
                     /locus_tag="VQ846_001744"
     CDS             7082..8278
                     /locus_tag="VQ846_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET3076282.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8414..8941
                     /locus_tag="VQ846_001745"
     CDS             8414..8941
                     /locus_tag="VQ846_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET3076283.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9018..9374
                     /gene="glnR"
                     /locus_tag="VQ846_001746"
     CDS             9018..9374
                     /gene="glnR"
                     /locus_tag="VQ846_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET3076284.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9411..10757
                     /gene="glnA"
                     /locus_tag="VQ846_001747"
     CDS             9411..10757
                     /gene="glnA"
                     /locus_tag="VQ846_001747"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET3076285.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10847..10966)
                     /locus_tag="VQ846_001748"
     CDS             complement(10847..10966)
                     /locus_tag="VQ846_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET3076286.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
BASE COUNT         3513 a         2036 c         2414 g         3044 t
ORIGIN      
        1 tagaggtgta ctattctagt ttcaatatac tatatatctg cagaatactc tttcagtttc
       61 attcagccta gtcttgctta ggaaaaagta aaaagccatc caattatgat ggatagcttc
      121 ttggttctta gatttgttct gcaaaaacct tttcagcaag gttcatagcg tggtctgata
      181 cacgagtgta gtgtgagatg atgtcgataa agttgacccc agcttgtgtt gaacattcgc
      241 ctttgttgag gcgtttgatg tgggtttttc tgagaacacg ttctatctta ttgattgctt
      301 catgacgttc tacaagactg cgtgcttttt caatatcatt gttttccaca ctatccagag
      361 catctttgat aaagtcacta gtttggcggt aaacttcctc taattctttc aaggcggcat
      421 cagaaaattc aacatttttc cgttgaagat agtcagtcag attgagtaga gcctccgtgt
      481 ggtctccaat ccgttccaaa tcacgggagg aatcaaggat attggtaagc acttcacttt
      541 ctttttggct gagagcttcg cttgaaaggg caatgagata acgtgttaat tgctcatcga
      601 tggtgttaat tgcttcttcg gttttatgcc ctttttcagc aactttttca tccaagtcaa
      661 tgatgtactt ataggaaagg tcaaaggctt tagcagcgta gtttcctaag tgcaagagct
      721 ctttcttagc atttcctaga gcgatagatg gggcctgttt gatgaaatgt tcatcaagat
      781 ataagggttc gtatttgaca acctcgtcct ctccaggaat aatcttggtt acaaagtaag
      841 ccagagctcc gataaatgga aattggacaa tggtgttggt aatattaaag gttccgtgag
      901 caaaggcgat ggtcatttcc ggtgctagat ttagcgtagc ttcaaaccaa tgaatcagga
      961 cagtaaaagg aactagaaaa ataacgcaga caactgttcc gataacgttg aaggcaacat
     1021 gagctcctgc tacccgttta gctgcaatat tagcccctaa agaggcaatg atggctgtaa
     1081 tggttgtccc gatattgtca ccaaatagaa ctggcaaagc tccctgtagg tcaattagat
     1141 tgccggcgta gaggttttgt aaaatcccaa tggtagccga agaagcttga attagcaagg
     1201 tcaagccagt accgacaaag acacccaaaa caggattctt acttagctca atcatatagt
     1261 ccttaaagac ctgtaaatcc ttgagtggag ccattgcgcc gctcatgaga ttgagggcaa
     1321 aaaagatacc accgacacca aagaggatgc gtccgatatt attgactgtc cgattttttg
     1381 taaaaaagag acagacggca ccgataaaga gcataggtag ggcatagtta cctaatttaa
     1441 aaccgatgag aaaggatgtg acagttgtcc caatattagc acccatgaca atcccgatag
     1501 cctgacgtaa ggttaagaga ccggcactga ccaggccgac tgtaataact gttacaccag
     1561 aactagactg aattagagca gtcatcccaa taccaaccag aactccaaag aaaggattac
     1621 tagtatattt gtcaatgtaa aaacgaaggc gatctccagc agcttgttgc aaaccgtctc
     1681 ccatggtctt gatactatat aagaatagcc ccagaccacc taaaaagtga aataaaattt
     1741 cctgccaatt aatggacatt tctttttcct ccgaaaaata atcgcggaat atctcctatt
     1801 ttattttaaa ggataaaagt aaatctaaca agtgttaatg tcaagtttgt aaaagaaaat
     1861 tataattgat aagtgtatta tttttaagag tttttaacct tattttagat ttgtgaaaac
     1921 agctattgtt ttttgtctaa aaatgtttta ggataaaaat gtgattatca tggttctaga
     1981 gtttgataaa aaactacaga atataaagcg ctttcttgaa aacaacaaaa tcaatctttt
     2041 aggaggagaa aatgaagaat ccattttttg aaagacgttg tcgttacagt attcgtaagt
     2101 tatcagtagg agcctgctcg ctgatgattg gtgctgtttt atttgttggt ccagccttgg
     2161 ctgaagaaac tgcagttcct gaaaatagcg gagctaatac agagcttgtt tcaggagaga
     2221 gtgagcattc gaccaatgaa gctgataagc agaatgaagg ggaacatact agagaaaaca
     2281 agctagaaaa ggcagaagga gtagcgacag catctgaaac tgcagaagca gctagcgcag
     2341 ctaaaccaga ggaaaaagca ggtgaggtgg ttgcagaaac accatctgca gaagcaaaac
     2401 ctaagtctga caaggaaaca gaagcaaagc ccgaagcaac taaccaaggg gatgagtcta
     2461 aaccagcagc agaagctaat aagactgaaa aagaagtcca gccagatgtc cctaaaaata
     2521 cagaaaaaac attaaaacca aaggaaatca aatttaattc ttgggaagaa ttgttaaaat
     2581 gggaaccagg tgctcgtgaa gatgatgcta ttaaccgcgg atctgttgtc ctcgcttcac
     2641 gtcggacagg tcatttagtc aatgaaaaag ctagcaagga agcaaaagtt caagccttat
     2701 caaacaccaa ttctaaagca aaagaccatg cttctgttgg tggagaagag ttcaaggcct
     2761 atgcttttga ctattggcaa tatctagatt caatggtctt ctgggaaggt ctcgtaccaa
     2821 ctcctgacgt tattgatgca ggtcaccgta acggggttcc tgtatacggt acactcttct
     2881 tcaactggtc taatagtatt gcagatcaag aaagatttgc tgaagctttg aagcaagacg
     2941 cagatggtag cttcccaatt gcccgtaaat tggtagacat ggccaagtat tatggctatg
     3001 atggctattt catcaaccaa gaaacaactg gagatttggt taaacctctt ggagaaaaga
     3061 tgcgccagtt tatgctctat agcaaggaat atgctgctaa ggtaaaccat ccaatcaagt
     3121 attcttggta tgatgccatg acctataact atggacgtta ccatcaagat ggtttgggag
     3181 aatacaacta ccaattcatg caaccagaag gagataaggt tccggcagat aacttctttg
     3241 ctaactttaa ctgggataag gctaaaaatg attacactat tgcaactgcc aactggattg
     3301 gtcgtaatcc ttatgatgta tttgcaggtt tggaattgca acagggtggt tcctacaaga
     3361 caaaggttaa gtggaatgac attttagacg aaaatgggaa attgcgcctt tctcttggtt
     3421 tatttgcccc agataccatt acaagtttag gaaaaactgg tgaagattat cataaaaatg
     3481 aagatatctt ctttacaggt tatcaaggag accccactgg ccaaaaacca ggtgacaaag
     3541 attggtatgg tattgctaac ctagttgcgg accgtacgcc agcggtaggt aatactttta
     3601 ctacttcttt taatacaggt catggtaaaa aatggttcgt agatggtaag gtttctaagg
     3661 attctgagtg gaattatcgt tcagtatcag gtgttcttcc aacatggcgc tggtggcaga
     3721 cttcaacagg ggaaaaactt cgtgcagaat atgattttac agatgcctat aatggcggaa
     3781 attcccttaa attctctggt gatgtagccg gtaagacaga tcaggatgtg agactttatt
     3841 ctactaagtt agaagtaact gagaagacca aacttcgtgt tgcccacaag ggaggaaaag
     3901 gttctaaagt ttatatggca ttctctacaa ctccagacta caaattcgat gatgcagatg
     3961 catggaaaga gctaaccctt tctgacaact ggacaaatga agaatttgat ctcagctcac
     4021 tagcaggtaa aaccatctat gcagtcaaac tattttttga gcatgaaggt gctgtaaaag
     4081 attatcagtt caacctcgga caattaacta tctcggacaa tcaccaagag ccacaatcgc
     4141 cgacaagctt ttctgtagtg aaacaatctc ttaaaaatgc ccaagaagcg gaagcagttg
     4201 tgcaatttaa aggcaacaag gatgcagatt tctatgaagt ttatgaaaaa gatggagaca
     4261 gctggaaatt actaactggc tcatcttcta caactattta tctaccaaaa gttagccgct
     4321 cagcaagtgc tcagggtaca actcaagaac tgaaggttgt agcagtcggt aaaaatggag
     4381 ttcgttcaga agctgcaacc acaacctttg attggggtat gactgtaaaa gataccagcc
     4441 taccaaaacc actagctgaa aatatcgttc caggtgcaac agttattgat agtactttcc
     4501 ctaagactga aggtggagaa ggtattgaag gtatgttgaa cggtaccatt actagcttgt
     4561 cagataaatg gtcttcagct cagttgagtg gtagtgtgga tattcgtttg accaagccac
     4621 gtaccgttgt tagatgggtc atggatcatg caggggctgg tggtgagtct gttaacgatg
     4681 gcttgatgaa caccaaagac tttgaccttt attataaaga tgcagatggt gagtggaagc
     4741 tagctaagga agtccgtggc aacaaagcac acgtgacaga tatcactctt gataaaccaa
     4801 tcactgctca agactggcgc ttgaatgttg tcacttctga caatggaact ccatggaagg
     4861 ctattcgtat ctataactgg aaaatgtatg aaaagcttga tactgagagt gtcaatattc
     4921 cgatggccaa ggctgcagcc cgttctctag gcaataacaa ggtacaagtt ggctttgcag
     4981 atgtacaggc tggagcaact attaccgttt atgataatcc aaattctcaa actccgctcg
     5041 caaccttgaa gagcgaagtt ggaggagacc tagcaagtgc accattggat ttgacaaatc
     5101 aatctggtct tctttattat cgtacccagt tgccaggcaa ggaaattagt aatgtcctag
     5161 cagtttccgt tccaaaagat gacagaagaa tcaagtcagt cagcctagaa acaggaccta
     5221 agaaaacaag ctacgccgaa ggggaggatt tggaccttag aggtggtgtt cttcgagttc
     5281 agtatgaagg aggaactgag gacgaactca ttcgcctaac tcacgcaggt gtatcagtat
     5341 caggttttga tacgcatcat aagggagaac agaatcttac tctccaatat ttgggacaac
     5401 aagtaaatgc taatttgtca gtgactgtca ctggccaaga cgaagcaagt ccgaaaacta
     5461 ttttgggaat tgaagtaagt cagaaaccga aaaaagatta cctagttggt gatagcttag
     5521 acttgtctga aggacgcttt gcagtggctt atagcaatga caccatggaa gaacattcct
     5581 ttactgatga gggagttgaa atttctggtt acgatgctca aaagactggt cgtcaaacct
     5641 tgacgcttcg ttaccaaggt catgaagtca actttgatgt tttggtatct ccaaaagcag
     5701 cattgaacga tgagtacctc aaacaaaaat tagcagaagt tgaagctgct aagaacaagg
     5761 tggtctataa ctttgcttca ccagaagtaa aagaagcttt cttgaaagca attgaagcgg
     5821 ccgaacaagt gttgaaagac catgaaatta gcacccaaga tcaagtcaat gaccgactta
     5881 ataaattgac agaagctcat aaagctctga atggtcaaga gaaatttaag gaagaaaaga
     5941 cagagcttga tcgtttaaca ggtgaggttc aagaactctt ggatgccaaa ccaaaccatc
     6001 cttcaggttc tgccctagct ccgcttcttg agaaaaacaa ggtcttggtt gaaaaagtag
     6061 atttgagtcc agaagagctt gcaacagcga aacagagtct aaaagatctg gttgctttat
     6121 tgaaagaaga caagccagca gtcttttctg atagtaaaac aggtgttgaa gtacacttct
     6181 caaataaaga gaagactgtc atcaagggtt tgaaagtaga gcgtgttcaa gcaagtgctg
     6241 aagagaagaa atactttgct ggagaagatg ctcatgtctt tgaaatagaa ggtttggatg
     6301 aaaaaggtca agatgttgat ctctcttacg cttctattgt gaaaatccca attgaaaaag
     6361 ataagaaagt taagaaagta tttttcttac ctgaaggcaa agaggcagta gaattggctt
     6421 ttgaacaaac ggatagtcat gttatcttta cagcaccaca ctttactcat tatgcctttg
     6481 tttatgaatc tgctgaaaaa ccacaacctg ctaaaccagc accacaaaac aaagtccttc
     6541 caaaacctac ttatcaaccg gcttctgatc aacaaaaggc tcctaaattg gaagttcaag
     6601 aggaaaaggt tgcctttcat cgtcaagagc atgaaaatgc tgagatgcta gttggggaac
     6661 aacgagtcat catacaggga cgagatggac tgttaagaca tgtctttgaa gttgatgaaa
     6721 acggtcagcg tcgtcttcgt tcaacagaag tcatccaaga agcgattcca gaaattgttg
     6781 aaattggaac aaaagtaaaa acagtaccag cagtagtagc tacacaggaa aaaccagctc
     6841 aaaatacagc agttaaatca gaagaagcaa gcaaacaatt gccaaataca ggaacagctg
     6901 atgctaatga agccctaata gcaggcttag ccagccttgg tcttgctagt ttagccttga
     6961 ccttgagacg gaaaagagaa gataaagatt aaatatcgaa aaatcttgtg aaatctttcc
     7021 ttatatttcc aaagtgtgat ataatagttt cgaataaaat aaataaagga gtttttgtaa
     7081 catggcaaaa cttactgtta aagacgttga cttgaaaggt aaaaaagtcc tcgttcgtgt
     7141 tgacttcaac gtaccattga aagatggcgt aatcactaac gataaccgta tcacagcagc
     7201 tcttccaact attaagtaca tcatcgaaca aggtggacgt gcaattcttt tctctcacct
     7261 tggacgtgtg aaagaagaag ctgataaagc tggtaaatca cttgctcctg tagcagctga
     7321 cttggcagca aaacttggtc aagatgttgt tttcccaggt gtcactcgtg gtgctgaatt
     7381 ggaagcggca atcaacgctc ttgaagatgg acaagttctc ttggttgaaa acactcgtta
     7441 cgaagatgtt gacggcaaga aagaatctaa aaacgatcct gaacttggta aatactgggc
     7501 atcacttgga gatggtatct tcgtaaacga tgcattcggt acagctcacc gtgcacacgc
     7561 atctaacgtt ggtatctcag caaacgttga aaaagcagtt gctggtttcc ttcttgaaaa
     7621 cgaaattgcc tacatccaag aagcagttga aactccagaa cgtccattcg tggctatcct
     7681 tggtggttca aaagtttcag acaagatcgg tgttatcgaa aacttgcttg aaaaagctga
     7741 taaagtcctt atcggtggtg ggatgactta cacattctac aaagcacaag gtatcgaaat
     7801 cggtaactca cttgtagaag aagacaaatt ggatgttgcg aaagctcttc ttgaaaaagc
     7861 aaatggtaaa ttgatcttgc cagttgactc aaaagaagct aacgcatttg ctggttacac
     7921 tgaagtgcgt gacactgaag gtgaagcagt ttctgaaggc ttccttggtc ttgacatcgg
     7981 tccaaaatct atcgccaaat ttgacgaagc tttgactggt gccaaaacag ttgtatggaa
     8041 cggacctatg ggtgtatttg aaaacccaga tttccaagct ggtacaatcg gtgtgatgga
     8101 cgctatcgtg aaacaaccag gagttaaatc aatcatcggt ggtggtgact cagctgccgc
     8161 agcgattaac cttggccgtg cagacaagtt ctcatggatt agtacgggtg gtggagcatc
     8221 aatggaactt cttgaaggta aggttcttcc aggacttgca gccttgacag aaaaataaga
     8281 ttttataaat aaatcaaaga agagagggat gaaagttcct cttttctttt gcttaaaata
     8341 aaaacgcttc ctctcaacta ttactcataa aaatcaccga tttatgataa aatggaaata
     8401 gaaagttgag attatgagtt attttaaaaa atataaattc gataaatccc agttcaaact
     8461 tggtatgcga acctttaaaa caggtattgc tgtttttcta gttctcttga tttttggctt
     8521 ttttggctgg aaaggtcttc aaattggtgc tttgacagcc gtttttagcc tgagggagag
     8581 ttttgatgag agtgttcatt ttgggacttc gcgtattcta ggaaatagta tcggtggact
     8641 ctatgccttg gtcttcttct tattaaatac ctttttccat gaagcctttt gggtgacctt
     8701 ggtagttgtt ccaatctgca ccatgttaac cattatgaca aatgtagcca tgaataacaa
     8761 agcaggggtt attggtggtg tagcagctat gttaatcatt accctatcaa ttccaagtgg
     8821 tgagacaatt tcgtacgtgt ttgtgcgtgt atcagaaacg tttatgggag tttttgtcgc
     8881 aattatcgta aattacgata ttgatcgtat tcgtctcttt ttagagaaaa aagaaaaata
     8941 atgttacatt ttataacatt atcaattgac gtttgtcttt ttttagacta taatagacag
     9001 aaagaaggaa attgtaaatg aaggaaaaag aatttcgccg aaatatggct gtttttccta
     9061 tcggcagtgt tatgaagttg accgatctat cggcgcgtca gattcgttat tatgaagatc
     9121 aagagttgat caagcccgat cgaaacgaag gaaatcgtcg catgtattcc ttgaatgaca
     9181 tggatcgtct gcttgaaatc aaagattata tctctgaagg ttataatatc gctgccatta
     9241 agaaaaaata tgctgaacgt gaagcgaaat ccaagaaagc ggttagtcag actgaagtac
     9301 gtcgtgcact tcacaatgaa ctcctccaac aggggcgttt tgcttcagta cagtcacctt
     9361 ttggtcgcgg ttaggcaacc gcaagtagtc atagattaag gagaaaactt atgccaatca
     9421 cagctgcaga tattcgtcgt gaagtcaagg aaaaaaatgt tacctttatt cgtcttatgt
     9481 tctcagatat tttgggaacc atgaaaaacg tcgaaattcc tgctacagat gaacagttag
     9541 ataaggtctt gtcgaacaag gttatgtttg atggatcttc tattgaaggt tttgtacgta
     9601 tcaatgagtc ggatatgtac ttgtacccgg acttggatac atggacagtc ttcccttggg
     9661 gagatgaaaa tggaagtgtt gcaggtctga tctgtgatgt ctatacaaca gaaggtgaac
     9721 catttgcggg tgaccctcgt ggtaatttga aacgagctct tcgtcacatg gaagaagttg
     9781 gattcaaatc cttcaacctt ggtccagagc cagaattctt cctatttaag ttggatgaaa
     9841 atggggaccc aacacttgaa gtgaatgaca agggtggcta ctttgatttg gcacctactg
     9901 accttgcgga caacacacgt cgtgagattg tgaatgtctt gaccaaaatg ggatttgaag
     9961 tagaagcgag tcaccacgag gttgcggttg gacagcatga gattgacttt aagtacgatg
    10021 aagttctccg tgcttgtgat aagattcaaa tctttaaact tgttgttaaa accattgctc
    10081 gcaaacacgg actttacgca acatttatgg cgaagccaaa atttggtatt gctggctcag
    10141 gtatgcactg taatatgtcc ttgtttgatg cagaaggaaa taacgccttc tttgatccaa
    10201 atgatccaaa aggaatgcag ttgtcagaaa cagcttacca tttcctaggc ggtttgatca
    10261 agcatgctta caactatact gccatcatga acccaacagt taactcatac aaacgtttgg
    10321 ttccaggtta tgaagcgcct gtttacattg cttgggctgg tcgtaaccgt tcgccacttg
    10381 tgcgcgtacc tgcttcacgt ggtatgggaa ctcgtcttga gttgcgttca gtggatccaa
    10441 tggcgaaccc ttacattgct atggctgttc ttttggaagt tggtttgtat ggtattgaaa
    10501 ataaaatcga agcaccagct cctatcgaag aaaatatcta catcatgaca gcagaagagc
    10561 gcaaggaagc tggtattaca gaccttccat caactcttca taatgctttg aaagctttga
    10621 cagaagatga agtggttaaa gctgctctcg gagatcacat ctacactagc ttccttgaag
    10681 ccaaacgaat cgaatgggca agttatgcaa ccttcgtttc acaatgggaa attgataatt
    10741 atttagacct ttactaatat agaagaaaga ttgcctgtgg gtaaacagtc acacagcagt
    10801 ctaagccatc aaatacctat aacccttaaa atagcgattt cggtcgctat ttatttttcg
    10861 aaaaatcctc ttgactcttt tgcgaattag atttatcatc agacgtgtag aagaaaatca
    10921 aggagtcact atggaaaaat tcaatttcaa aaacaatatc gggcaagaaa acaagttact
    10981 tcaaatcgaa atctacaagt ttactaa
//
LOCUS       DAVBNO010000060        10939 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVBNO010000060 DAVBNO010000000
VERSION     DAVBNO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.060"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(65..1201)
                     /gene="dnaJ"
                     /locus_tag="VQ846_001749"
     CDS             complement(65..1201)
                     /gene="dnaJ"
                     /locus_tag="VQ846_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET3076287.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFATAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1406..1759)
                     /locus_tag="VQ846_001750"
     CDS             complement(1406..1759)
                     /locus_tag="VQ846_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076288.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDYTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(1772..3595)
                     /gene="dnaK"
                     /locus_tag="VQ846_001751"
     CDS             complement(1772..3595)
                     /gene="dnaK"
                     /locus_tag="VQ846_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET3076289.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(4075..4599)
                     /gene="grpE"
                     /locus_tag="VQ846_001752"
     CDS             complement(4075..4599)
                     /gene="grpE"
                     /locus_tag="VQ846_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET3076290.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(4626..5660)
                     /gene="hrcA"
                     /locus_tag="VQ846_001753"
     CDS             complement(4626..5660)
                     /gene="hrcA"
                     /locus_tag="VQ846_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET3076291.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRIADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(5824..6333)
                     /locus_tag="VQ846_001754"
     CDS             complement(5824..6333)
                     /locus_tag="VQ846_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET3076292.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            6941..7241
                     /locus_tag="VQ846_001755"
                     /pseudo
     CDS             6941..7241
                     /locus_tag="VQ846_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818342.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
     gene            7436..9274
                     /locus_tag="VQ846_001756"
     CDS             7436..9274
                     /locus_tag="VQ846_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET3076293.1"
                     /translation="MKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQII
                     HRLRKAGLAKRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYL
                     GLYQQLTGEDGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGM
                     TATPKETKNASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKV
                     DTNGQLIEDRYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQSNARFDKTIVFCVDIDHA
                     ERMRAALVKENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTT
                     GVNAKTCRLIVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDF
                     DGEPVKVLETGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLIT
                     ESLTDYTRKNILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQE
                     IDDFDLLLKLAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGEL
                     ESIETLKLPEFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            9287..10750
                     /locus_tag="VQ846_001757"
     CDS             9287..10750
                     /locus_tag="VQ846_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET3076294.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            10750..>10939
                     /locus_tag="VQ846_001758"
     CDS             10750..>10939
                     /locus_tag="VQ846_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HET3076295.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT         3326 a         2143 c         2146 g         3324 t
ORIGIN      
        1 gatacgactc acatatatac ggcaaggcaa cgctgacgtg gtttgaagag attttcgaag
       61 agtattattc tccatcaaag gcatctttta tatggtcaaa gaagcctttt ttctttggat
      121 ttactttcaa gtcaccagca gtcgcgaatt ctttcaaggc tactttttgg cggtcgttca
      181 agcctgtcgg tgttacgaca ttaacagtaa cgtattggtc accaactgca ccgccacgaa
      241 ggctcggtgc ccccttacta cgtaggcgga atttcttacc agtctgagtt ccctctggaa
      301 taaccaattc aacatcaccg tgaacagttg gaatatctac tgtatcacca agagccgctt
      361 ggacaaagtt gaggttgaga ttgtagaaga tagtcgttcc ttcacgttca aacttgtcgc
      421 tagcttctac agaaactact acatacaagt caccataagg tccaccgtta aagcctgctt
      481 caccttgacc agcgaggcga atttgttgac ctgtttccac accagcaggg attttcacat
      541 gtacgctatg agcttgtttc tcatgacctg ttccatgaca ggttgtacat ggatatttga
      601 tttcttttcc tcgaccgtga cagacatcac aggttacttg gcgacgcatc ataccaagag
      661 gagtctgcgt atcgacgtta atgacaccag cgccatgaca gcgtccacaa gtgactggac
      721 ttgtccctgg cttagcacca gagccattac atgtacgaca gccagcttca cgatgatact
      781 taacttcctt ctcagttccg aagatagctt cttcaaatgt caaattgaca cgatactgga
      841 gatcatctcc ttggcgagga gcgtttggat tgcgcgaaga accgcctccg ccgaagaaac
      901 ttgagaaaat atcctcaaaa ccaccgaagc cacctgcccc attgaaacca ccgaaaccac
      961 cagctccacc aaaaccacca ttggcgcctg cagcaccata ctggtcatag gcagcacgtt
     1021 tttggtcgtc actcaaagtc tcataggctt cttgaacttc cttgtacttg tcctcagcac
     1081 caggctcctt gttgatatct gggtgatatt ttttggaaag cttacgataa gcctttttga
     1141 tctcgtctgc cgaagcgttt tttgacaccc ccagacgatc ataaaattca gtattgttca
     1201 tacaagatac caagaccgta aaattcgact gaaaaatagg aaatctgacg ctggagcgac
     1261 gctcctagac agatttctct tttttccgag aatttaggtc gggttcaatt cctttctttt
     1321 atattgatga gataatactc cagccactcg gccctatccc tttttaccga aaaacagagg
     1381 ctgggttgca acctctgtgg ttagattatt catttgaaat aaaaatggtt gtattatctc
     1441 tagtagtaat cttcaaagac cttttagcta aatcaaactc aaatttatta tagtgtgggt
     1501 cttgtgtttt atataggtgt aactcctgac atatatgatc cgaaaagtaa atcataacat
     1561 ctttaaacac agaagtgtag tcatatttac tcttaccaac agctacttct gtaaattctt
     1621 tataaatgac atttccattt ctttccacag tcaccagcat ttctgttgga ttataatcca
     1681 aattaaataa ttcttcaaaa aactttatct tttcatgata atctgcaatg tcagaaagat
     1741 tatttttatt aacactcata acactagccc cttatttttc cgtaaactct ccgtctacga
     1801 cgtcatcgcc tgcgtttcct gttgcttgtg cgccttctgc tccttcttga gcttgttgcg
     1861 ctgctgcggc ttgttcgtag agtttaacag caagtccttg agctttttcg ttcaatgctt
     1921 caagtttcgc tttcatgtcg tccaagttgt tgtcttcttg agctttctta aggtcatcaa
     1981 gggcagcttg ggcagcgtca cgttctgcgt cgaagccttt accttcagtt tccttgattg
     2041 tcttttcagt cgcaaagatt gcttggtcta cttcattacg aaggtctact tcttctttac
     2101 gtttcttatc ggcttcagcg tttgcttctg catctttcat catgcggtcg atttcttcgt
     2161 cagtcaaacc tgagttcgat tggatgacaa tagtttgttc tttttgagtt ccaaggtctt
     2221 tggccttaac agacacgata ccgttcttgt cgatgtcaaa tgttacttcg atttgaggaa
     2281 ttccacgagg tgcagctggg atatcagtca attggaagcg tccaagagtc ttgttatctg
     2341 ctgccattgg gcgttcacct tgaagaacgt ggatatcaac ggctggttgg ttgtctgctg
     2401 ctgttgagaa gacttgtgat ttagatgttg ggattgtagt gttgcgatcg ataagttttg
     2461 taaatactcc acccattgtt tcgataccaa gtgacaatgg cgttacatca agaaggacaa
     2521 cgtccttgac atcaccagta atcacaccac cttggatagc cgcacccata gcaactactt
     2581 catcagggtt tactgatttg tttggttctt taccagtttc agctttaaca gcttcaacaa
     2641 cggcagggat acgagttgaa ccaccaacaa ggataacttc gtcgatttct gacaagctca
     2701 aacctgcatc tgaaagggct tgacgaactg gaacttttgt acgttcaaca aggtcacgag
     2761 tcaaatcatc aaatttcgca cgagtcaaag tcatttccaa gtgaagaggt ccagcctcac
     2821 ctgcagtgat aaatggcaag ctgatttgtg ttgaagttac accagaaagg tctttcttcg
     2881 ctttttcagc cgcatctttc aaacgttgca ttgccatctt gtcagtagac aagtcgatac
     2941 cgttttcttt cttgaattct gctaccaagt ggtcaatgat tttttggtca aagtcgtcac
     3001 caccaagttt gttgtcccct gcagttgaca atacgtcgaa gacaccgtca cccaattcaa
     3061 ggatagagac gtcgaatgta ccaccaccaa ggtcaaatac caagattttt tcttctttat
     3121 cagtcttgtc caaaccataa gcaagagctg ctgcagttgg ttcgttaaca atacgttcta
     3181 cttcaagacc agcaatttta ccagcgtctt ttgttgcttg acgttgagcg tcgttgaagt
     3241 aagccggaac tgtgataaca gctttggtta ctttctcacc aaggtagtct tcagcgtagc
     3301 ctttcaagta ttgaaggatc atagctgaga tttcttgtgg agtgtattct tttccatttg
     3361 cagaaacttt ttcagaagtt cccatcttag atttgataga gataactgta tctgggtttg
     3421 tgactgcttg acgttttgca gcatcaccaa cgatgatttc tccgtttttg aatgagacta
     3481 cagatggagt tgtgcggttt ccttctgggt ttgcgatgat tttgctttca gttccttcaa
     3541 gaactgcaac tgctgagttt gttgtaccta agtcaatacc gataatttta gacatgtgtt
     3601 tttctccttg ttagttaatt ttctattttt ctttttgata ctcttcttaa gtggcgacgc
     3661 cgtcagagct tgacttcgtc aatctctttg actaaacttt gagcctaagg tctcaaaatt
     3721 tgcgcaaata gcgccacggc gaagagtatc gtgtttggtt cgcttcgctc actattacaa
     3781 agccaaacat attttatctt tcttcatagt ttattgtcgt ttcggacaag ttttctatta
     3841 tgtaaattgc gacacgaaga gtcgaaatcg attttatttc gacgacgagt tagtaaggaa
     3901 gctaggcaaa cgccatagcg attgccgttt tctgacgagt tgctttagct accgtcagaa
     3961 ttgccgaatc gaacacgccc taagctctga gtaaaaaaga tagattttct tgtgttcatc
     4021 gaacacttcg ttaatctcct atttttactg cgagcttttt acgggctttg tatcttagtt
     4081 atacaccact accattgctg ggcgtaggat gcggtcatgg agtttgtagc ctttttgaaa
     4141 gacttgagcg atggtatcta ctgggtgttc atcgtctgct gggagagttt ggatggccat
     4201 atggtagtta tggtcaaatt cgccatctgc tgcgatttct tcaattcctt cttctttcaa
     4261 agcgtgaatc aagctttctt gcaccatccc caagcccttc ttcacatcat ctgtcaaacc
     4321 ttcaactgca agtgcacgct caaggttgtc aagagatggt aagattgctt ttgccaagtc
     4381 ctggctacga taacgttgca agttttgacg ttcttcattg gcacggcgtt ggatattttg
     4441 catttctgca tgagcgcgaa gatatttgtt ttcgaactca tctgcacgtt catttgccaa
     4501 gtccaactca gacttttcag gagttgtttc ttcagctgtt tccacaactt cctcttcttg
     4561 aacttcttct acttcttcat tttttatatc ttgggccatg ttcgcctcct ttaatgattt
     4621 caatcttaat gtacttcgta atgattactg ctgaggtagc ggtaaaaatc tgtcaacttc
     4681 atggtcaaaa cacggttgac cacattgatt tgattgatta gctgttggta atccagatta
     4741 actggaccga taatggctag aattccaact ccccgataag gaatgaggaa cttactacta
     4801 atcaccgcta ggtcagctaa acaggactct tgaccgtctg caatacgaac attttgcatc
     4861 tgatcctcac gcaacccctc acgaatctcc aaggccacct tttgcggttg gtcaaagaac
     4921 tgataggctg ctagattggc aaaattcaag agattgacct tgcccgccat cacaatgttt
     4981 tcgttgaaca tttccttaaa gatgtgttca aagagatcga tgacattatc cgttgttgta
     5041 aagtaacgct ggataatctg cggaatctcc gtccgaatct tgtagtgaat atctaaaacg
     5101 gtgtgaccga ggaaacgttc ctgaatgatg ctcttcagtt tcagcaaatc ctcctgcaag
     5161 aagttccttg gaatcagaaa ctgactagta accgttcgcg actcgtctag ggtaaatacc
     5221 gccaaggctg tatgttgccc caaaacaacg atatcaaagg ctgtcaaacg ttgcctgctc
     5281 ggctcaacat ccagtgccac taccgtacag ccactcaggt ctgttagcaa gttagcagcc
     5341 tcttgcagaa tatcctccaa tttgaagaat tcctgatcaa aggctttgac aatctcatat
     5401 acctcatttt cagccagccg gtgaaaatcc agtgagtgtt tcacatagta ctgaaaacca
     5461 gcaacacttg gcatccgacc gcttgaagta tgagccttct caagcaaccc ttgtttttct
     5521 agttccgcca tgtcattacg aatggttgca ctgctagagt taatagactc ttgcaaggct
     5581 tttgatccga caggttcgtg cgttttggta aagatgtcaa taatcagatt taaaatatcc
     5641 tgctgacgct ctgtaaccat ctcatcactc ctctctgtct gatcaattag cactcaatgc
     5701 atccaagtgc taacttatga ttctattata cacccttaga agagaatgtc aagacaaaaa
     5761 ctcaaaaaat tagcactctt ttatcaagag tgctaaaaat caatctgaag acctagaacc
     5821 ttgctattca tctactcggt atttcctttt tagtctgaaa taaccatgaa ttagataaga
     5881 aatcagaaaa gcatataaaa caaaaataag aaagaaagac atagaaagat cctctctaaa
     5941 caaactcgta caaacaacaa gaccacctaa gaaagtagct gtacaggaac gaagtctaga
     6001 tcgcataact tcccatttga gccccttact catatagact tgatcctctg gaagtaaatc
     6061 agaagacgat ttacgaagaa tcgaacaaga acctgctcct atcaattccc aacctctatc
     6121 tctaaaatct tgcagttcat gcttatattt tttaagaaat ctagaatcat agatacggta
     6181 gatgacatcg tctggttgac attggtcaaa atagaacaaa ccaaaacgac tcgttctata
     6241 cctccaacct ttcaaatgca tctcatgtaa atattcttct ttcttgtcca aatcaacaat
     6301 ggtgaaaatc cgaaattcta ctctgctatt cattgtctta ccccaaaatt agaaaacatg
     6361 cctagcgtta tttattagat aattctttcc acttttgact caatctccaa aaaatataag
     6421 aaatctgaat cgcaaaaact atcaataaaa cccaatctat tatgaaaatc aaaaacactt
     6481 cccaactgaa agaactacct ccagtgacaa actttgagaa aaacggtagt agagctaaaa
     6541 agagaagcaa aataggaagc atccgcattg ttaaaatccg tttggcataa aaaaatcttt
     6601 atttaaacga aaatattatg gcaaaattta cgccagtttt tgaacggctg atgtagatat
     6661 tttatacttt caaaatgttt aaatgtgatt atttattttt gaaaaatagc tcaccagccc
     6721 gactgaaagt gcttatagaa tgataataag tcgcctgccg aaaacagcgg aaaatagcgg
     6781 tgttatgcgg agataatctg acgcgatgcg aaagtatatt gcatacttat tttcaacaat
     6841 ttagcagagt atttttataa gtgtgatata atagaagtat aatttgttct gatagtttat
     6901 tttatggaga agtagatttt tagaatgcgg agggttcaat atggttgagt ttataaagtc
     6961 taagaaagaa atgagtgagg aggatattaa agcaaatttc atcactcctg ctattgtatc
     7021 caaaggatgg aaaaatggtg agcatatcgc ttacgaagaa tacttcactg atggtcgaat
     7081 tgaggttaga ggagataagg ctcgtcgtaa agaaggaaaa aaatcagact attcactgta
     7141 ttaccaattt ggaactcgaa ttgcaattgt tgaggcaaag gataataaac acagcgttcg
     7201 agcaggatta caacaagcta ttgaatatgg agagatttta gatgttccat ttgtttattc
     7261 ttcaaatggt gatggcttta ttgaacacga ccgtatcacg agagaagaac gtgagctgga
     7321 gttagacgaa ttccctactc gtgaagaatt attttctcgt atgacgaagg aaaaaggatt
     7381 gacgtacgaa attacagaag ctatctaaac tccatactat acagacgcct tctcaatgaa
     7441 aacgccacgc tattatcagc aaatagctat caaccgtact attgaaacag ttgccagagg
     7501 acaaaaacga gtaatgtttg tgatggcaac aggaacgggg aaaacgttca tggcttttca
     7561 aattattcat cgccttcgaa aagctggttt ggctaaacga gttttattct tagcggatag
     7621 aaacatctta gtagaccaaa cgatggctga agactttaag ccattcgaaa aggtaatgac
     7681 gaaaattaca ccaaaacttt tgactgctcc tgaaaaatta aattcttttg aaatttatct
     7741 agggctttat cagcaactaa ctggtgaaga tggaactgaa acacattatc aaaaatttga
     7801 caaagacttc tttgatttaa tcgtaattga tgaagcgcac cgtggttcag ctaaggaaaa
     7861 cagtaactgg cgtaagataa ttgattattt cagttctgcg acacagattg ggatgaccgc
     7921 tactcctaaa gaaaccaaga atgcttccaa tacggaatac tttggtgagc caatctatac
     7981 ttatagttta aaacagggaa tcgaggatgg ttttttggct ccatatcgtg ttatgagggt
     8041 taatttagat gtggatgtgg atggttatcg tccagaaact ggaaaagttg atactaacgg
     8101 acaattaata gaagataggt actacggcag gaaagatttt gataaaacca ttgtcattga
     8161 tgatagaacg caaagagttg ccaagtttgt ttctgattat atgaagcaaa gcaatgcacg
     8221 atttgataaa acaattgttt tttgtgttga tattgaccat gccgagcgaa tgcgtgctgc
     8281 acttgtaaaa gagaatctag acttagtcca agaagactat cgttatgtca tgcaagtaac
     8341 tggtgacaac gctgaaggaa aagctcaact ggataacttt atggatgtca attctaattt
     8401 tcccgctatt gtaacaacgt ctaaattatt aacgacagga gttaatgcta aaacatgtcg
     8461 tttgattgtt ttagactcta atatccaatc catgactgaa tttaaacaaa ttattggtcg
     8521 tggcacacgt ctttatcctc aaaaggggaa agaatttttt acgattattg attttcgaaa
     8581 tgttaccaat ttgtttgctg accctgattt tgatggtgag ccagtgaagg tgctggaaac
     8641 aggtgcgaaa acagtcagtg gttccacgcc cggtttcgta gatgaggaag gtgacccagt
     8701 agaaaaatat atcgttacag acaagcaggt taccattctt aattctactg ttcaagtatt
     8761 ggatgaaaac gggaaactga ttaccgaaag cctgaccgac tacactcgga agaatatctt
     8821 aggtagctac gccactttga acgattttat cacagtttgg catacggcgg ataaaaagaa
     8881 gcttatctta gacgaacttt ataaaaaagg agtttatcta gatgctattc gagagtcgga
     8941 gggaatatca gaacaagaaa tcgatgattt tgatttactc ctaaaacttg cctatggtca
     9001 aaaagaatta accaaaacgg aacgtatcaa taaactcaaa caaagcggat atttatataa
     9061 atatagtgag gaagcgcgtg ctgttttgga aattttactg aacaaataca tggataaagg
     9121 tattggagaa ctcgaaagca ttgaaacatt aaaacttcca gaatttcaga tatatggtgg
     9181 aaccttcaaa atcatcaata cttattttgg agataaaaaa cgatatttac aagcaattaa
     9241 agaattggag caagagctat ttacagtagc ttaatgaaag gaaagtatgt caattacatc
     9301 atttgtaaaa agaattcaag atatcactcg aaacgatgct ggtgttaatg gtgatgctca
     9361 acgtattgag caaatgtctt ggttattatt cttaaaaatt tatgatagcc gtgaaatggt
     9421 ttgggaatta gaagaagacg agtatgagtc aattatccca gaggaattaa aatggcgaaa
     9481 ttgggctcat gctcaaaatg gggaacgggt attgacaggc gatgaattac ttgattttgt
     9541 caataacaag ttattcaaag agttgaaaga gcttgaaata acttcaaata tgcctattcg
     9601 aaaaacgatt gttaaatcag cttttgaaga tgcgaacaac tatatgaaaa atggcgtctt
     9661 gttacgccaa gtcatcaatg ttattgatga agttgatttc aatagccctg aagatcgtca
     9721 ttcgtttaat gatatttacg aaaaaattct taaagatatt caaaatgctg ggaactcagg
     9781 agaattttat acgccacgtg cagcgactga ttttattgcc gaagttcttg acccaaaact
     9841 tggagaatca atggcagacc ttgcttgcgg aacaggaggc ttcttgactt cgactctgaa
     9901 ccgtttaagt agtcaacgta aaactagtga agataccaaa aaatataata cagctgtttt
     9961 tggtattgaa aagaaagcat ttcctcatct tttagcagtt acaaatctgt ttcttcacga
    10021 aattgatgac cctaaaattg ttcatggaaa tactttggag aaaaatgttc gtgaatatac
    10081 ggatgatgaa aaatttgaca ttattatgat gaatccacct tttggagggt cagaattaga
    10141 aacaataaaa aataactttc cagcagaatt acggagttct gaaacagctg atttatttat
    10201 ggctgtcatt atgtatcgtt tgaaagaaaa tggtcgtgtt ggagttattt tacctgatgg
    10261 ttttctattt ggtgaaggtg taaaaactcg cttgaaacaa aaactggtag atgagttcaa
    10321 cttgcatacg attattaggt tgcctcatag tgtctttgca ccgtatacag gaatccatac
    10381 gaacattctt ttctttgata aaacaaagaa aacagaagaa acttggtttt atcgtttaga
    10441 tatgccagat ggttataaaa atttctcgaa aactaagccg atgaagtcag aacacttcaa
    10501 tcctgttcgt gactggtggg aaaatcgtga agagattctg gaaggtaagt tctacaaatc
    10561 taaatcattt acacctagtg aattggctga gttgaattat aatttagacc agtgtggttt
    10621 tccaaaagag gaagaggaaa tcttaaatcc ctttgagttg attcagaatt atcaagcgga
    10681 aagagcaact ttaaatcata agattgataa tgtattagct gatattttgc agttgttgga
    10741 ggacaaataa tgacaccaga acaacttaaa gcaagtattc tccaaagagc gatggaaggg
    10801 aaattagtgc cgcaaaatcc caatgacgaa cctgcaagtg aattattaaa gagaattaaa
    10861 gctgaaaaag aaaaacttat cagtgaagga aaaatcaaac gagataaaaa ggaaactgag
    10921 atatttcgtg gtgatgatg
//
LOCUS       DAVBNO010000061        10756 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVBNO010000061 DAVBNO010000000
VERSION     DAVBNO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.061"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..270
                     /locus_tag="VQ846_001759"
     CDS             <1..270
                     /locus_tag="VQ846_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3076296.1"
                     /translation="QHDSYHRFLESKGIQASMSRKGNSQDNGRMESFFGILKSEMFYG
                     YEKTFKSLNQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(386..1189)
                     /locus_tag="VQ846_001760"
     CDS             complement(386..1189)
                     /locus_tag="VQ846_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET3076297.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1464..2219)
                     /locus_tag="VQ846_001761"
     CDS             complement(1464..2219)
                     /locus_tag="VQ846_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HET3076298.1"
                     /translation="MSKHPHYELLNLIGYGLAKFAKLFIKEFQYSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNSRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYVEIVKM
                     LLASEGKQTGITIIEKPIIRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDACLY
                     GDGYDFQVDIQEHSYLAEVKGIRKPKGRVRLTAYEFEKAKEFQSDFILSLVTNLDDIP
                     KLVLVDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2289..4940)
                     /locus_tag="VQ846_001762"
     CDS             complement(2289..4940)
                     /locus_tag="VQ846_001762"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET3076299.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(4933..5511)
                     /locus_tag="VQ846_001763"
     CDS             complement(4933..5511)
                     /locus_tag="VQ846_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET3076300.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5511..6071)
                     /locus_tag="VQ846_001764"
     CDS             complement(5511..6071)
                     /locus_tag="VQ846_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3076301.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSIYFNHRHEDDVMEIGY
                     TLHSDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSQRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWGVI"
     gene            complement(6058..6507)
                     /locus_tag="VQ846_001765"
     CDS             complement(6058..6507)
                     /locus_tag="VQ846_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HET3076302.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(6920..7315)
                     /locus_tag="VQ846_001766"
     CDS             complement(6920..7315)
                     /locus_tag="VQ846_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET3076303.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQTAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASHFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            complement(7411..7665)
                     /locus_tag="VQ846_001767"
     CDS             complement(7411..7665)
                     /locus_tag="VQ846_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET3076304.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(7675..9009)
                     /locus_tag="VQ846_001768"
     CDS             complement(7675..9009)
                     /locus_tag="VQ846_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET3076305.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQIFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(9009..9242)
                     /locus_tag="VQ846_001769"
     CDS             complement(9009..9242)
                     /locus_tag="VQ846_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET3076306.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            9273..9347
                     /locus_tag="VQ846_001770"
                     /pseudo
     CDS             9273..9347
                     /locus_tag="VQ846_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9555..9695)
                     /locus_tag="VQ846_001771"
     CDS             complement(9555..9695)
                     /locus_tag="VQ846_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076307.1"
                     /translation="MKFFWGLLAIIFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(9692..9874)
                     /locus_tag="VQ846_001772"
     CDS             complement(9692..9874)
                     /locus_tag="VQ846_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076308.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKDDNAVVGLGKLFIAD
                     KLMDTARWLIKPEDKK"
     gene            complement(9876..10655)
                     /locus_tag="VQ846_001773"
     CDS             complement(9876..10655)
                     /locus_tag="VQ846_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076309.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIKGE"
BASE COUNT         3148 a         2392 c         1993 g         3223 t
ORIGIN      
        1 caacacgatt cttatcatcg gttcctagag agtaagggaa ttcaagcatc catgtcacgt
       61 aagggcaaca gccaagacaa cggtaggatg gaatctttct ttggcatttt aaaatccgaa
      121 atgttttatg gctatgagaa aacatttaaa tcacttaatc aattggaaca agccattata
      181 gactatattg attattacaa caacaaacga attaaggtaa aactaaaagg acttagccct
      241 gtgcaataca gaactaaatc cttcggataa attaattgtc taactgtttg gggtcagtac
      301 attttaacac ttgatactat gcgttttatt gtgggaatgt ttacttcatt ttttcctaaa
      361 attcaatgtt tactcagtat ttggattatg aaaaatcgag gtctaaatct agatacattt
      421 tttctgaaga caaatcattt tgaccaccga gcaagagatt ttcaaaaaaa gctgttaaaa
      481 actcaggacg tcgctgtaaa atctttgcat tatctaatac caaggcatca cgaaaatact
      541 tggaatgttg ctgaaatggt atattatcaa tatcaaaacc aaactcacga agatactgaa
      601 tcaaaaagac cgttactgtc cgagtgtttc cttcgcgaaa tggatgaatc tgccagattc
      661 ctgaaataaa atgctggatt tgtttaacca catccgcctg agttagtgtc gcatatgcaa
      721 cttgtttttc ctgattaaaa tcataatcta aggtcatttg aatcatggag taatcagagt
      781 acacaacact ttcaccattc aaaacaggtt cattctttgt gatattggtc tgacgaaatt
      841 gccccaccgg aatagagggt tcaaatatat cttgaaacaa ctccttatga atagcaagta
      901 aggtcgcagg actaaagcta aagcctcttc gagacaatag ttctacaata cgtagagaaa
      961 ccaagtctgc ctcttccgta cttgcatcaa tagtatggtg ataagccgtt gcatcctcat
     1021 aaacctgctc ataagtcagt tctccccggg actgtttctc agccaaagat tccatatacg
     1081 ctgatggcac tagattgtca actttctgca gaccaaaacc tatccgccat aaatcacgct
     1141 tcgcttcata agacaagttt ggattgtcaa tgttgtaagt tgattgcata gaaatatcct
     1201 ttcaattgtt tttcttcatt accagtaaat cacgaaattg gttggaaact ggcttaaact
     1261 ataaaaagtc ctcacaatag gttctatgat atatatatac ttcatgtaaa taataaaaga
     1321 gttataatca gcactactga taaattcaat accgatttcg ccatcttaaa acttagtcaa
     1381 acgaataccg ttgatttcca gttcattaag tcgttgacct gtcaagactt ctcgttcctt
     1441 caactggaaa agttaagggg gaactaatac aaatcctcta cgcttctata ttcaatgatt
     1501 tcatttttta taatattctt tttaaactca aaatgtttta gaggattatc aactaatact
     1561 aactttggaa tatcatctag gttggtgacc agagataaaa taaaatctga ttgaaactct
     1621 ttagctttct caaattcgta ggcagttaaa cggacacgac ccttaggttt tcgaatccct
     1681 ttaacttcgg caaggtagga atgttcttga atatctactt ggaaatcata tccatcacca
     1741 taaagacatg catcggttaa ctgtccacct tgaaattttt cttctttatc aaaatgaagg
     1801 ataaaataat tctctgcctc catccccgtt tcctgtagac gtttgaattt agttcgaata
     1861 attggttttt caattatagt aatgcctgtt tgctttcctt cactagcaag taacatttta
     1921 acaatttcaa cataactatg aacatcttca tttccaaaca tcatatcaat taaatcttta
     1981 cgaaaacggt aaacttcagc tttctgccac caaccttttc gactattgtc aaaataagga
     2041 tcaaataaat ccattctatt ttttacaact ccagttgttt cagcaattcc caaagaaacc
     2101 acatagcgat aaaactccga cttactagaa tattgaaatt cttttataaa aagcttggca
     2161 aacttggcaa gaccgtagcc aattaaattt aacaattcat aatgcgggtg tttagacatc
     2221 actttctcca tcaatagata gtaatttcat tatcccaaaa aacacggcat tacaccatgt
     2281 ttctgtgttt atttcaccaa cttcttcatc tcatcaatac gtgctacggt cgcatcatac
     2341 ttggcttggt agtcagcttg tttgtcgcgt tctttttgga cgacttctgg tttggcgttg
     2401 gctacgaagc gttcgttaga gagcttctta ccgaccatgt ccagttcttt ttgccattta
     2461 gcaagttcct tgtcaagacg agccagttct tcttcgacat tgaggaggtc agccaatggc
     2521 aggtagattt ctgctcctgt aatgacgctt gacatagcca gttcaggtgc agggatggtt
     2581 gatgcgattt ccaagtgttc tggatttgta aagcgtttga tatagttgac attgctgtta
     2641 aagaaggctt ccaagtcgct atcgcttgtc ttaacaagga tggtgatagg cttgcttggt
     2701 gctacgttta cttccgcacg cgcattacga acggcacgga tcaagtcttt gagactttct
     2761 acaccagtat gagccgcaag gtcttcaaag gctagattaa cagttgggta tgcagctgtc
     2821 acgatagaac cttctgagat ttgtccaaag atttcctctg tcacgaatgg catgattggg
     2881 tgaaggagac gaaggatctt gtccagagtg taaaggagaa cagatcgagt aatgacctta
     2941 tcgtcttcat tgtcgctgta aaggacttcc ttggtcaatt caacatacca gttggcaaat
     3001 tcttcccaaa tgaagttgta aaggatatga ccagccacac caaactcgaa cttatcaaag
     3061 ttttcagtaa cttttgcaat ggtttcgttg agattgtgga gaatccagcg gtccgtcaca
     3121 ttaccagcct cacctgttgc aacttttgtg acattgtcat gcgccacatc cagcgtcaaa
     3181 ccttcattgt tcatgaggat atagcgagaa atgttccaaa ttttgttaat aaagttccat
     3241 gaagcatcca ttttctcgta agagaaacga acgtcttgac caggagctga gccgtttgac
     3301 aagaaccaac gaagactatc tgtaccatat ttatcaataa catccattgg gtcaatgcca
     3361 tttccaagag atttagacat cttacgtcct tcttcatcac gaattagacc atggataagg
     3421 acgttttgga atggttgacg gcctgtgaat tccaatgact ggaagatcat acgagacacc
     3481 cagaagaaga tgatgtcgta acctgttacc aaggttgaag ttgggaaata acgtttaaag
     3541 tcttctgagt caacttcagg ccagcccatg gttgaaaatg gccagagggc agaactgaac
     3601 caagtatcca agacgtcttc gtcctgagtc catccgtcac cttctggagc ttcttcgccg
     3661 acatacattt caccatcagc attgtaccag gcagggattt ggtgacccca ccaaagctga
     3721 cgagagataa cccagtcgtg gacattttcc atccattgaa ggaaggtatc gttgaaacga
     3781 ggtgggtaga attcgacctt gtcctctgtg tcttggttgg caatggcgtt cttagccaat
     3841 tggtccatct tgacgaacca ttgagtagac aagcgtggct caactacgac acctgtccgt
     3901 tctgagtgac cgactgaatg gacacgtttt tcgattttga caagggcacc gatttcttcc
     3961 aacttagcaa cgactgcctt acgagcttca aaacgatcca tgcctgaaaa ttcaaaggca
     4021 agctcattca tagttccgtc gtcgttcatg acgttgactt gtggcaagtt atgacgttgg
     4081 ccaaccaaga agtcatttgg atcgtgggca ggtgtgattt tcacgacacc agtaccaaac
     4141 tcaggatctg cgtgctcatc tccaacgatt gggatgagtt tattagcgat tggaaggatg
     4201 acgtttttac caatcaagtc cttgtagcgc gggtcttctg gattaaccgc aaccgcaacg
     4261 tccccaaaca tagtctcagg acgagttgta gcaacttcaa gggcgcgtga accatcttcc
     4321 agcatgtagt tcatgtggta gaaggcacct tcgacatcct tgtgaatcac ctcaatatca
     4381 gaaagggctg tgcgagctgc tgggtcccag ttgatgataa actcaccacg gtagatccag
     4441 cctttcttgt aaaggtccac aaagacctta cgaacagctt ttgacaaacc ttcgtcaaga
     4501 gtgaaacgct cacgagagta gtctacagag agccccatct tgccccattg ttccttgata
     4561 gtagtggcat attcgtcttt ccattcccag actttcgtca agaaagactc acgaccaagg
     4621 tcatagcggg aaatgccctc accacgcaag cgctcctcta ccttagcctg agtggcaatc
     4681 cctgcgtggt ccatcccagg aagccaaagg gtatcaaaac cttgcatgcg tttttgacgg
     4741 atgataatat cttgcaaagt tgtatcccaa gcgtgaccaa ggtgaagttt acctgtaacg
     4801 tttggtggtg gaataacgat tgaataaggc ttagcctttt gatcgcctga aggcttgaaa
     4861 acatcagcat caagccattt ttggtaacga ccagcctcaa cctcggctgg attgtattta
     4921 gatgaaagtt ctttagacat gtgtgtgtcc tttctctatt ttgtttattt tattttgaat
     4981 ttgcttagca gcttcttctg cagacaaatt cgtattattt attttaaagt agtggtgcaa
     5041 ctcattcggt tgatattggg aatttaattg aagtgtttca gcggtctcta aaatttctct
     5101 ttcagatacc tcaatatgtc gttttaaggg tttgtgcttt aatcgattct ccgttcgatt
     5161 tcgacgtatg cactcttcaa gacttgtttc caactcaaca aaaagaatct cttgatgata
     5221 atcatccaac aaattctgaa tttgctttaa atatatcaga tgatactgat ttgaaaaatc
     5281 aattacatct gttaaaatta ctgatcgctg atttcttgca cttgctccaa ggaatgaaaa
     5341 agtgattcca cgaacaaatt cccacatttc ctctgtatag tcctgataga tttctagtgc
     5401 aaaatcaatg gcttgatggt tataaaatag ggtagcatcc gtcagtcgag ataattcttg
     5461 accaatggtc atttttcctg atgctggagc accaatgatg aaaagatgca tcaaatcacc
     5521 ccccactcac tcttcagcaa ggcatatctc aaatcatcac agcagttgcc ttgagcatct
     5581 ttacggtctc ttatgcgagc ttcgagggta aagccaagct tttccgcgac tcgttgactt
     5641 tgaaggttat atccaaagca agttagttca atcttgtgaa gacccaaatc tttaaaggct
     5701 aagtcaatca aggcacgcgc agcttctggc acataacctc gcccccaata gtctgagtgt
     5761 aaggtatagc cgatttccat cacatcatct tcatggcgat ggttgaaata gatagagcca
     5821 acgatcttat ccgttccttt gacgacaatc ccgtagcctg ctgggagatt ttccttttga
     5881 ttacgctccg gaagaatgtg ctccagataa taaatctcat cttccaaggt cttgactgga
     5941 ggaaaacctg ctggataggc gacctctggc aaactagcat agtcaaagat atcctcagca
     6001 tccgccacag tacggactcg taaaacgaga cgttctgttt cgattttatc tggcagctca
     6061 gttcttgcca tccttcttcc tcgctttttt gatgaaactg cccttcatat ctacacgctt
     6121 gtccagatag cgataaacgc gctgatatcc atctcccatg aaataggttg gggcaaacag
     6181 ttgattttta aaatgtccct tttcatccag gagttctggg gcaacaagtc gctcaagaat
     6241 cttggcaaag atgtggcaaa taccgtcttc ctcaacaatc ctatctaccc gacaatctaa
     6301 aacaagtgga caggcgtcta aaataggagt ctgagttcgt tcagaaattt cataatgcac
     6361 tcccaaacgt tccaatttct cctgatgact gataaaacca gcctgctcca tcgcaagcat
     6421 agaagtttca tcagaaatat tcacagtaaa tttttgatac tgtttgatct gctctgcggc
     6481 attctctctc gcaacgactc caatcacaac ccaatctcct agactataag aggaactaca
     6541 ggtcgtgatg ttatagccaa aattctaatc ttgatatcct aaaataaaaa caggaaaacc
     6601 ataatatagt ttacttgtgt taaaagattg cttcataaca accccctttg actaagacgt
     6661 aaaagaaaag ccctgccatc tacatgacag ggacgaatgt gtttatccgc ggtaccaccc
     6721 aatttcgggc agttgcccgc aactctcgtc tttaaagcaa aaagacactt ttatttcaat
     6781 ttttcatcca ttagcaacca gttttgacac atttgtggac ttctcagcgc cgccactttc
     6841 tgtaaaatgt gggattcaaa acacctctaa tggctctatt gtagcaagat tttttcggaa
     6901 atgcaaggct caagaaaaat tacttgaact tgatgccatt ttctttcaat ttcttgatgg
     6961 tagctgggcc gattcctttc aagtcaagga gttctttttc agtccagttt ttgaaatgtg
     7021 aagcagactt gattccttca tcatagaaag tcttggcacg atcaagtgga agtccaccca
     7081 aatttgctgc aaaatcttct acagaattag ctactttttg agcttgctct ttggtagctt
     7141 ctactgcttg ttcaactttt ttagaaacag ccttaccagc tgcgcttgct gtctgttctg
     7201 cacgcttcac gacttttttt gtctcatcta caaccttagt cacagtactt gaaaaggcac
     7261 ctgcgcgacg gaggccattt cgtaattgtt ttttacgatt gagtttcttt gacatgataa
     7321 aatcctttca ataaaaaatc cctgctctga caaggattta acataaacat tctatcaaga
     7381 ttttagtgaa aaatcaagaa tctttctcgt ttaataattt cgctcaccaa acaagccttc
     7441 tggcaaatca tggaatccct tgcctacttt gaagttttca aacttaccaa gcaagaactg
     7501 ataagcatcc aagcgactct cgtagcgaat catggcatct ttggcacgct cgtcctggtc
     7561 ttcttcgtaa actttttgac tttccttgtg cgccatatcg ttaatcatga tttgggtatt
     7621 gacccaagct tcaaattcct tcataaattc ttgttcgtaa ctcatttttt ctccttattc
     7681 taatccacga aaatagtccg tatcaatctc acggtagggc tggatatcct gaacatactc
     7741 caacactcct tggaattctc cgtcttcatc gtgtactgca gcataggtga tgtggacaaa
     7801 cttacctcgc gactcagact tgaaccacat ttcatacttg tccttggtcc cctcacgaag
     7861 tcccttcatg atagccttga ccttgtccaa atacttaggc ggatgacaga gttcgacatt
     7921 gcgcccgact tgggacggcg tccgtttgaa aatcatctca tcagctggcg tattgtcatt
     7981 gtaatactgg aaaatatctt ctttattgac aaaggtaatc tccataggga gatgattgag
     8041 gatgagattg gcctgctcga ctgaaagata gccattacca aaagcctgtt gactatggcg
     8101 gtccagcaca gcttcctttt ccttaggggt aaaggtaatg gtaaaatggc cttctggcgt
     8161 atctatgact tgttgaactt gaccttctgc cgtatctagt tgtacaggct cctctgcaat
     8221 cttttcctca ataaagatct gtcgttctgg cacccatttc tctgacggac ggatgatggc
     8281 atagccatag gcatcactct cctccgcaat ctgaagccag tcatcctgag taaaagactc
     8341 aaggagaatc atgagcagga tggactcttc cttgaaaatc atactttcaa actctgtcgc
     8401 aaaagcttca aaagcttcct ttacactgct aattgacact tctggtagtg acttggctgt
     8461 cgttagagct gtttgaaaga gttccctaat ctgatcatcc actccccaca taactttggg
     8521 aggtgaatcg tgtccatagc gctccatgat aggaaagaag agttcttcct tacgttggta
     8581 atggatgtca aattgaccca caagtcccat ctgacgcacc aaacccttac gcatctccgc
     8641 cagcatttcc tcgtcttcca tagactcata ggtatctaac aatctacgaa tgcgaatcaa
     8701 ggccgcacgg agagccagat tttcttcttt gaagacacga actgggtgac ctggatgctc
     8761 agtatctgaa acttcgacac ctttgatagc atttttaaaa agattggcat ggacatcaca
     8821 gagttccata acatcttcaa aagtgacgcc cgaatccgag ttcatcagct cgtgctccat
     8881 aagggaaatc tcaatggctg agacacccgc aaaggtcgca tcaaaacgct cctgaaccga
     8941 ctcaggagag gcgccattat gcaattctaa taaaatatcc cgtaggacat gaatccgttc
     9001 atctgccatt agtctaatcc aatcacttcg tagccattcg cttccagtgt gcgtacaatc
     9061 ttgtccatag gagttcctgc tagcttagaa ccctgtttaa gtgatacttt acgaccaact
     9121 gtattgcgca ttaagggatt ggcaaggggt ttaaaaccca actccactag aatttccaag
     9181 acttctggat gcttgtccac cacttctgca acaggaattg acacatcgat gatattgtcc
     9241 atgacgacct ccattatatg ttctaatact caatgaaaat caaagagcaa actaggaagc
     9301 tagccgcagg ttgctcaaaa cagtgctttg agattgtaga tagaactgac gaagtcagct
     9361 caaaacaccg ttttgaggtt gcggatagaa ctgacgaagt cagtaacata tatacagcaa
     9421 ggtgaagctg acgtggtttg aagagatttt cgaagagtat aaaatgattt tactgcttat
     9481 attataccat atggaaagag aataaaaaac tagcagtgga gttcctgcta gttctctgcg
     9541 acttggatta ccatctagat aagtttaaag aaccaatcca atatcttgta aaacagcaat
     9601 tggactgcaa aagagatgat catccaaaag aatttcacaa tcccaataat cggtttgata
     9661 aaaataatgg caagaagtcc ccagaaaaat ttcatttttt atcctctggc ttaatgagcc
     9721 accgagccgt atccattaac ttgtcggcaa taaaaagttt ccccagaccg acaacggcat
     9781 tatcatcttt ctttatcgtt ttcattactt ttacaccttg cacggtcaaa aagtaattac
     9841 cataaatttt agcaaaagct ttgataggat tcatattact ctcctttgat gatttcagcc
     9901 tcttttcgga ttgtttcaac atcttcttga tattgaactt cactatagtt gactagcttg
     9961 gataattctt tctgcaagct ttcccccatt ggtttcatag ttgcaaaggt taaaccacct
    10021 gaaatgatac cacccaagat aggaataaat ttccccattc ctttggccag ccctcccttg
    10081 gtaagattca caccaaatat tcttaagact ttcttcaaaa taggatacca aagcgtcttt
    10141 gttaaagctt tattaggtac tgttttcatt acctgtttgg caattgttat accaccagca
    10201 cgtagcaaag cagcagttcc attcaccccc aacatgacgc ctagataaag caaaagggta
    10261 ttttgagcat cttcactcaa ctcatcgcgt gaagcccaaa gatcctcata accataaata
    10321 taacctaatt cttgggccaa tttcagagag aaaccataaa attgagccac atcagctgga
    10381 atggtaattg ccatagcaag ccctccaggt aatcctgcca aaacagaagt cccactcgct
    10441 aataaaacat tatccctaat acaggcatta gccactcgat ctaagatttc ttgagataag
    10501 agagacgttg gaccttgttc caataaggta ggaatgtcct gtggacccaa ttctttggaa
    10561 aacttatcca ctaaaaattt cgaacgatca accttaacaa caggtagttt caccacttgt
    10621 tgcaatactt gcatagctaa gtcttgttca gccatataca aactccttat ttcttttttg
    10681 caccttatca tattatattt cctacaaaaa aagcattttc taattagata acattaaaaa
    10741 atacagaacc cctttt
//
LOCUS       DAVBNO010000062        10754 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVBNO010000062 DAVBNO010000000
VERSION     DAVBNO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10754
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.062"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(117..1541)
                     /locus_tag="VQ846_001774"
     CDS             complement(117..1541)
                     /locus_tag="VQ846_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET3076310.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2085..4364)
                     /gene="pulA"
                     /locus_tag="VQ846_001775"
     CDS             complement(2085..4364)
                     /gene="pulA"
                     /locus_tag="VQ846_001775"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET3076311.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4476..6434)
                     /gene="ligA"
                     /locus_tag="VQ846_001776"
     CDS             complement(4476..6434)
                     /gene="ligA"
                     /locus_tag="VQ846_001776"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET3076312.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLLVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6527..7705)
                     /locus_tag="VQ846_001777"
     CDS             complement(6527..7705)
                     /locus_tag="VQ846_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET3076313.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQTIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7785..8648)
                     /locus_tag="VQ846_001778"
     CDS             complement(7785..8648)
                     /locus_tag="VQ846_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET3076314.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGLALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8651..10552)
                     /locus_tag="VQ846_001779"
     CDS             complement(8651..10552)
                     /locus_tag="VQ846_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET3076315.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRTILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEQTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKVTAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3221 a         2371 c         1915 g         3247 t
ORIGIN      
        1 tgtactattc tagtttcaat atactataat accaaaaagt tcgtattttt tctataatta
       61 ttattatttt atagcaaaaa aataaaacca ggaaaacaat ttcctgattt tacacgttat
      121 ttcacatcaa aaacaatgga tttgacattt gtcatcgctt caatgctata tttaatccct
      181 tgcactccag ctccagaacc tttgacacca aggaacggga aattatctgg accacgctgg
      241 gttttattat taatgtggac tgtacctact tcaagttttt cagcaatttc aaaggctttt
      301 ttgaaatcat ttgtaaagac tgatgattga aggccgaatt cagattcgtt ggcaaaggca
      361 atagcttcct ctacactagc cacacgaatg attggtaaaa caggaccaaa tggctcttcc
      421 catgccactt tcatatcttt tgtaacttgg tcaaaaagca ctggccagag gagattgccc
      481 tcacgtttga ttggtgtaag agcctgagcc tctttttctt gtgcatcctc aatcaagccc
      541 caaatgaagt cggctgaagc attgtcaata acaggtgtaa tatcagcatt gtcaaatgga
      601 tcaccaactg ttaatttaga aacttcttcc tgaagcaaag tagctaattt atctgctaca
      661 ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt gtcctgagta gctaaaggct
      721 cccgcaacaa tttgcttggc agcatgttcc aaatctgcat cttctagtac aagagctgca
      781 tctttcccac caagttccaa catgatagga cgcataccag ctaaacgacc aatacgttct
      841 ccaataggag ttgaacctgt aaagttgatg aagttgactt ctttgtgctc aatgatataa
      901 tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa cacctgccgg aatccctgct
      961 tcttcaaatg ctttagccaa caagagtcca gaaatggaac cttgtgttgg tggcttaaac
     1021 atgaccacat tccctgcaat caaggcaggt gcaattttag aagcagataa attaactgga
     1081 taattaaagg gagcaatcgc tagcacgata ccaactggtt cacgacggac aacagccagt
     1141 ttgtttttac ttgctgcctc aaaaccacca ccttccattg cttgtccagt gatacggaga
     1201 ccttcctcag cagcataacg aatcaagtct gctgtacgca ctacttctcc aattgctgct
     1261 ttaatccctt ttgctacttc tttggcaagg atagtaccaa tttcttcctt atcgcgttct
     1321 aaaatagctg ctgttttatg caaataagcc gcacgttcaa ttgctgataa agctcgccat
     1381 gctggcaggg ctgcacgcgc agcttgcata gcctcatcag cttcagtctg agtcatggct
     1441 ggaactgtac ccaattcttc ttgattgatt ggtgaataaa tcgtaatttc ttgttcagat
     1501 gatttccatt ttccatttac taaattctga tatcttgtca aattcttgtc ctcctgaaaa
     1561 tgattaagat atattttacc aaattttcag aaaaccacaa ccctttttgc gaaatttttg
     1621 agattatttt cacaagcttg atttttcaaa gtatactcaa taaaaataaa aaaatccact
     1681 acgtcaaggc gaggctaatg tggtttgaag aaattttcga agagcgtgaa tgagtatcat
     1741 ctatagtaaa ataaaaaaac tgaacaattt ggtttgggac agtcaaacca atttctcaca
     1801 atgttttaga aacacgggtg tgctattcca atttcaggct actataactg tcatagattg
     1861 ctgaaacaaa gtctaggtaa aagtcttcat aataaaaaga cctcctatca aatgttcaaa
     1921 aactttgata ggaggtcttg tttcgtgaaa atatttatca aattttctat acaagtgagc
     1981 tgttagccag gttctttcta ttctttcaat ttcaatgaat ggatttttta ccaatactca
     2041 taactgggaa tttttctgtg taaaaatagc gagatagatg gtatttataa aacactcaag
     2101 acagctagac taatatcatt taaaacatta tcttcttttg agcgactgtt ggttaccaac
     2161 atagctaaat ttcctgcatt ttcaaattga tagggttctg atttagcatt cacaaccacc
     2221 aagaggtgtt ctttgccgtg aacttcatag ataaggcagc cgctatgttc aatcgccgaa
     2281 tgcacaaaga catgatggta aatttcatca tagctagagt aagaaaaggc accagttttt
     2341 gtcttcaatc ggatgacttg acggataaac tcaatactgt cttgacgctc attaatcaag
     2401 ttccagttca cttggttcac actgtcagga gcattatagc tattcatcgc acgctctcta
     2461 tcatcatggg tcaactcacc attttcacca gtcgcaacca gtttggtacg accaaattct
     2521 tgaccgattt ccataaaggc catcccctgc atgagcagat tcatggctgt ggcagtttcg
     2581 accttgcgca tgatttgctc tgaactttgg tctggatgaa gggttgccaa taaatcgtga
     2641 agattgtaat tgtcatgggc ttctacatag ttaagcacct gatttggatg tgtatagctt
     2701 cctaattcac gacttcctag gattgcttta gctagaattg gctctgtcgc agcaccactg
     2761 acaaaacctg acttgatagc accataaact tctccccctt tgacagcatc gcgctgattg
     2821 tcattaaaga aaccaatatt tggcatctgg taggcattgt ccttcttggc cttatcataa
     2881 ggggcaagac ctgttcccat atcccatcct tctccataga ggataatgtt ggagtcgatt
     2941 tcatccaagc tttgacgaat catctgcatg gtcttgacat catgaatccc catcaagtca
     3001 aaacggaagc cgtcaatatt atattcctgc acccagtata gaagagaatc aatcatatac
     3061 ttgcgaaaca tttcgtgttc actggctgtt tcatttccaa cacccgttcc attctggaag
     3121 gtaccatctg gattcatacg ataatagtaa tcagggactg ttgtttggaa tggtgcatca
     3181 acaactgaga aggtatggtt atagactaca tccataatga ctccaatacc cgcatcgtga
     3241 taagcttgaa ccatcacctt caaatcacga atgacctgag ctggatcatc tggattagtt
     3301 gaaaaactag tttctggcgc gttatagttt tgtggatcat aaccccagtt gtaggttaca
     3361 tttccatcct catcgtattc tttatgacgg tctgcaattg gttgcaattg aacataattg
     3421 tagcccagct tcttgatgta atcaaaagca gttgactggc cgtattggtt aactgttcca
     3481 gcctgagcag cacccaagaa agttcctcga agatgttcat ctacacccga tgtaggtgat
     3541 ttagtcaaat cacgaatgtg catttcacag ataactgcct tacatggatt ttccaagcgc
     3601 caagtagcct ccgaaccgtg cttaacctcg aagttttcaa cttgcttttc tacatggctc
     3661 agaatagctg aacgtttgcc atcagggctg gtcgcgattg tataaggatc acgtgtcagt
     3721 gtttggtgat gagggaattg gacttgatac tgataagcct tacctaccaa atcttcctca
     3781 acatccaaac tccagacacc gattgtattg tccttatgat tataagagta gctattgcct
     3841 cttttcatct caaaagtctt ccaaacgggt gcatcattag cagctgattc ataaacgaca
     3901 acttgcactt ctgtcgctgt aggtgaccag agggaaaaat gagcctgatt gtcctctaca
     3961 cggcaaccca attctccttg gtaaccccaa tgatggtcaa aactagcact gttaatggcc
     4021 ttatcaaagg caaaaggatt ttgattttta tagaaaggac tggcaatagc aggattttca
     4081 gagtaataaa tcctatcatc gccttccaaa atccagactt ctgttaatag gggatagtga
     4141 ttaaaacgga tagaatattc tttactagtt tgacctgtat gaaccacaaa attcaagctt
     4201 tctataacat gtgaacttgg gtgttcaaag ctaaataaag ctccaaaata atcttctttg
     4261 taggttagca aatcaattcg ttgatcctga ctttttacaa aggagcaagt gtcatattca
     4321 ccattcttgc gatggtaatg aatgcgcata gggtagttat acatttttta tttttccttt
     4381 ttactttgtt tctatttcac taataaattt ttgtcaatct cgtctcaatt aacagacata
     4441 gtcatattct ctaaactctg tttttaaacg atccattaca aactttctag ccatgcctca
     4501 tctctgacct ggataccaag ttcttgtgct ttttgcagtt tacttccagc gtctgcacct
     4561 accacgacga ggtcggtctt tttagaaaca ctacctgtca ctttggcacc cagactttcg
     4621 agtttacttt tagcttctga gcgcttgagt cgttccaatt ttcctgtcaa taccacggtc
     4681 aaacctgaca aggccgcatc cgctactacc gtctgtcctt tatagtccag attgacccca
     4741 gtttctttca attctctgag cagaatttca gagccttctg tcgcaaaata agtctgaaga
     4801 cttttggcaa tcacgccacc tagactttca atactagcca cttcctctga atctgcctga
     4861 tacagatttt caattgaatg gaaatattga agtaaaagct gactagcctt gcttccgaca
     4921 tgacgaattc ccaaaccaaa taagagcttc tcggcagaat tttcctttga tgcttggata
     4981 gcctgataca gtttagcagc ggacttttcc ttaaccccct ctaaaaggag gaaatcctct
     5041 tcttgcaaac gataaatatc cgccacatcc ttgactaaat tagcagcaaa aagcttctca
     5101 acaatagatg gaccaaggcc tgtaatattc atagcatcac gagaagcaaa gtgaatcaag
     5161 ccttccatga tttgagcagg gcaacgcgga ttgatacaac gtagggccac ttcatcttca
     5221 aagtgcaaca agtcagagtt acaacttgga cagtttgtag ggatatctag tttttcttca
     5281 gaaacccgtt tggactctac cacacgtaaa acggcaggga tgatgtcacc agccttatat
     5341 acaatgaccg tatcgtcttt tcggatatct ttttcagcaa tataatctac attgtgcagg
     5401 gtcgcacggc taacagtcgt accggcaagt tgtactggtg ttagattagc agttggagtt
     5461 acaacaccgg tacggccaac tgtccagtca actaataaga gttgagcttc tttttcttca
     5521 gcagggaact tgtaggctac tgcccacttt ggagccttaa cggtaaaacc aagttcttct
     5581 tgacttgcta ggtcgttgac cttgattacc actccatcaa tatcgtaagg tagattttcc
     5641 cgttcctgtc ctacttcttg gataaaattc cagatttcat ctatgttttc agccaagatt
     5701 cgcttaggat tgaccacaaa acctagttgt tctaggtact tcaaaccctt ttcttggcta
     5761 tcacgagttg aaggactggc ttcttgatag agaaacgttg caagattacg ctttgcaact
     5821 actgctgtat ccaactgacg cagagttcct gctgccgcat tacgaggatt agcaaattca
     5881 ggctctccat tttcttggcg cgcttggtta acttggtcaa aggaagcgcg tggcatgtaa
     5941 cattccccac gaactgtgat atctagttct tctggcaaag tcaaagggat gtccttaaca
     6001 cgcttgaggt tttctgtgat attttcacca attgaaccat ctccacgtgt taccccagca
     6061 accaaaatcc ccttttcata agtcagcgag atagataagc catcgatttt cagctcacaa
     6121 atataggtcg gatgagccac ttccttacga acacgcgcat caaaagcatc tagctcctca
     6181 cgtgaaaaag catcctgcaa actataaaga ggatactgat gactgtattt ttcaaaacca
     6241 tctaaaacct tgccaccaac acgatgagtc ggactgtctg ctagcacttg ctctggataa
     6301 gcagtttcta actcgaccaa ctcacggtaa aggcggtcat actcactgtc tgaaaccgag
     6361 ggattatcgc tggtatagta ctcagtcgca tagcgattga gcaaagcgac taactcattc
     6421 attcttttat tcataagacc attttaccat aaaacaagcc ctcctcacaa acgagaaggg
     6481 cggaaaaaac acttagtttg aaattatttt tgaaactcaa gcaaccttat atcaattttt
     6541 caaaatgagt tcgaacataa ataaacgata tacaagacaa gatgataaca ccacttccaa
     6601 ttatcaggaa agaagagaga tgtacacttg gcaagactgt cataaatcct tttgcaatag
     6661 gcataaatag aatagctaag gtaaaaattg tactcagtac tctcccaaga aattcgctct
     6721 caaccttggt ttgtacttga gtaaaaaagt gaatattaaa aatcgtcata aacaattcac
     6781 aaactaaatt tccagaaaag gaaagaaaag ttggaagtgg taatcccatc ataaaaactc
     6841 cgacacctgt caaagccagt aaaatcaaaa gattataaat attagcttta attttactag
     6901 ctagaagagc cccaatgatg gaaccaatag cccccatagt taaaatactt gcataggctc
     6961 cttctgaccc gtaaagctga ttcgaaaagg gaagtagaaa ttcaaaagct gcaaaaaaga
     7021 aattaacgct ggaagctacc agcaaaagga agaaaatttc ttgctgatgc cagatatagt
     7081 gtaacccatc cttgatatct acaaaaatat ctctcccagt aaaagccttt ttctcttgaa
     7141 cttttgcttc ctcttttgga aggaaagcca ctagaacaaa agcaatgaaa aaagtcagcg
     7201 agtctagcag tagcgtcata tggagacttg caaactgtaa aacaaggaag gaaagaacag
     7261 gagagctaac acctacaacc tgcaaaacca gctctaagcg agaattatag atcacaatct
     7321 catctttctc caccacttca gttatgatag ctttattggc tgtgcgagaa aaggcaaaag
     7381 caatagtctg cacaatgtta gcaacaatca aagcgccaat catccagcta tcattcctta
     7441 tgaaagaaat agccagacaa agaatcccac aaacaagatc tgccgtcatt aaaatcttac
     7501 gacgagaaaa acggtctgaa ataactccgc caaagggatt gacgagaata gatgtgacga
     7561 gctcagaaat ctgatacatt cctaaaactg tctgtcctat agtccccata gaagccaacc
     7621 agacactatt tccataatca tagagcatat ttcccatttt attgatagcc ccacggctaa
     7681 tcaactgcac tgcatagcga ttcatattaa agctcctctc aaattttgaa actattgtat
     7741 caaaaccgaa aggagctttt tattttttcc cttatttggg aaaattaact tttgacaaat
     7801 ttttcgtagt gttcctgata ataggctact tgctctggaa gacctaacac atcaaaaata
     7861 tgcatggcct cttgcatctg cttacagcct tctttacact gtcctttttg atataaggcc
     7921 aaacctttta aataatggaa aacattacgc tcataaagct taataccttt gtcaataatc
     7981 ttctctgtat aagcctcaaa atagttggca ttataaaaag aagaatgctc taaacaatgc
     8041 tggtaacaat tgagggccaa aattaacact aatctcttat ggcgactaat ctcttggtaa
     8101 aattcctccc tctccataac ttctctacca atccgagtga catagtctac atcgtagaaa
     8161 ctatagaggt taccgaaaag aatcaactca tacatggtcc attcttctgt tttgaagaga
     8221 taatctgcta ccttatccaa atcatcctgc ttcatatcat aactcgcatc tctttgacaa
     8281 atcagacctt gtagcaaaat ccagttcagc tcaaaataaa ggggagtcgt cgaactctta
     8341 gacttttcaa gttgttctct ttgaagcttt tgaaaacctg caatatcgtt tgaatagtaa
     8401 agtgggataa tctgtgccat catagacaca tgttcatgat tatgaaaatt ccttgcctta
     8461 tccatgaaat tttcgattgt tacatgaatg ttatccaaaa tctcaaagaa acgggagact
     8521 gccaggtcag actccccaag ctcaaagcga gataactgag aggtagagca ggattcgcct
     8581 gctgcttcct ttaaagaata atttccactt gttcgaaatt cacgaaatac ttttccaaga
     8641 tgttccatct ttacacctgc tctgataatt cttcccactc aagcatagct tcttcctgac
     8701 gatggctgat tttgtccagc tcagcctgta attccatgag tttgtcggca tcgtttgttt
     8761 ccaacatttg ttcagaaatg gcttggcttt gactttctag ctcttcaatt tcagcttcta
     8821 gactttcgat ttgtcgcatg agtttgcgaa cttctttttg actttctttc tgggcctgat
     8881 agtcattgac tggacttgct tcctttgctt gattgctagt tgaagcttcc tcagtctgac
     8941 tcatttctgc tgttactttc ttctcaacat agtagtcgta atctccaagg tagagagttg
     9001 aaccattctc agacaattcc aaaacatgag ttgccacacg attgataaag taacgatcat
     9061 gactgacaaa aagcaaggta ccatcaaagt caatcaaggc attttctagc acttccttac
     9121 tatcaatatc caagtggttg gtcggctcat ccagaatcaa aaagttattg ttttccatag
     9181 acaatttggc taaaagcaaa cgagcttttt cgccaccaga tagcatgccg actgattttt
     9241 taacatcatc tcctgagaaa aggaaggctc caagacggtt gcggatttca acttctggtg
     9301 tcagtttgaa atcattccag agttcatcca gcaccgtatt acttggtgtc agcttgcttt
     9361 gggtttggtc atagtaacca acctcaacat tagcgccaaa gcgcttttct cccttgataa
     9421 acggaatttg atccacgatt gatttgataa aggttgattt tccgatacca ttgggaccaa
     9481 cgatagcgac agcattcatc ttacgaagat ctaggttgat aggttgtgac aagacttccc
     9541 cgtcataacc aatagcagta ttttcaacag tcaaaacaac attgcccgac gtttgttcag
     9601 actggaaggt catgttggct gctttcttgc cagcttcagg cttgtccaaa cgctccattt
     9661 tttctagttg tttacggcga gattgagcac gtttagtcgt tgaagcacga actagattgc
     9721 gattgacaaa gtcttccaga gcagcgattt ccttctgttg cttttcatag ttttttgcct
     9781 cagtaactag cttttgctcc ttcaattcga caaaacgaga gtaattcccc acatagcgat
     9841 ccaaggaatg cttggtcaaa tctagcgtaa ttgtcgcaac cttgtccaag aaataacggt
     9901 cgtggctgac gataatgagg gcaccgctat agtttaccaa gtaattctct agccaggcga
     9961 tggtttcaat atccaagtgg ttagttggct cgtccaagac caagagattg ggcttttcaa
    10021 ggagcatttt ggcaagtgcc aaacgagtat tttgaccacc agaaagctca gcaattttca
    10081 tctgccacat agactcgtca aacttgaatc cattcaaaat cgttcgaata tcagcttcat
    10141 aggtaaagcc acctgcttgg cgaaaattct cagataagcg gtcataatct gacatcagtt
    10201 tatccaaatc ctcaccagac ttttcaccca tctccagctc catctgacgc agttgtctct
    10261 ccgtccgacg caaattatta aagacatgaa gcatttcatc gtagatggta ttttcagact
    10321 caaaacggct atcttgggct aggtaagaca gagaaatatc ttttttctta ttgatttctc
    10381 cgctagttgg ctcctcttct ccaactaaaa tcttcaaaag agtagactta cctgcaccat
    10441 ttttcccaac aagggcaatc cgatctcgtt catcaacctg caggttgata ttatcgaaaa
    10501 gaacctctcc tgcaaaagaa cgttcaattt tattagcttg taaaataatc ataagagtag
    10561 tatagcatgt ttccctaagg cattcaagat aatcgtaagt cttttagtac aacttttata
    10621 acataaaata aactaaatta tgtatatttt atattagatt cttcactatc ttgttggatt
    10681 ttctaaccag ctaatcttgt ttcaaatagt tatcgcacaa gcctattatt taattctttt
    10741 cattatttac gtac
//
LOCUS       DAVBNO010000063        10686 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVBNO010000063 DAVBNO010000000
VERSION     DAVBNO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.063"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            85..366
                     /locus_tag="VQ846_001780"
     CDS             85..366
                     /locus_tag="VQ846_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET3076316.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGIDLLGYTTWS
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            498..1442
                     /gene="manA"
                     /locus_tag="VQ846_001781"
     CDS             498..1442
                     /gene="manA"
                     /locus_tag="VQ846_001781"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET3076317.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1433..2937)
                     /locus_tag="VQ846_001782"
                     /pseudo
     CDS             complement(1433..2937)
                     /locus_tag="VQ846_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3042..3782
                     /locus_tag="VQ846_001783"
     CDS             3042..3782
                     /locus_tag="VQ846_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET3076318.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSETAQYTSDAIAAYVQTNAE"
     gene            complement(3811..4266)
                     /locus_tag="VQ846_001784"
     CDS             complement(3811..4266)
                     /locus_tag="VQ846_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET3076319.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4286..5461)
                     /locus_tag="VQ846_001785"
     CDS             complement(4286..5461)
                     /locus_tag="VQ846_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET3076320.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDNQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDEVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5520..6365)
                     /locus_tag="VQ846_001786"
     CDS             complement(5520..6365)
                     /locus_tag="VQ846_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET3076321.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6490..7047
                     /locus_tag="VQ846_001787"
     CDS             6490..7047
                     /locus_tag="VQ846_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET3076322.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7066..7533
                     /locus_tag="VQ846_001788"
     CDS             7066..7533
                     /locus_tag="VQ846_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET3076323.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7622..8251
                     /gene="upp"
                     /locus_tag="VQ846_001789"
     CDS             7622..8251
                     /gene="upp"
                     /locus_tag="VQ846_001789"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET3076324.1"
                     /translation="MGKIEVINHPLIQHKLSILRRIDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8425..9015
                     /gene="clpP"
                     /locus_tag="VQ846_001790"
     CDS             8425..9015
                     /gene="clpP"
                     /locus_tag="VQ846_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET3076325.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAHETLEYGFIDEIMANNSLN"
     gene            9094..9342
                     /locus_tag="VQ846_001791"
     CDS             9094..9342
                     /locus_tag="VQ846_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET3076326.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9443..10603
                     /locus_tag="VQ846_001792"
     CDS             9443..10603
                     /locus_tag="VQ846_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET3076327.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEVAEVVKP"
BASE COUNT         3384 a         2106 c         2278 g         2918 t
ORIGIN      
        1 tcttctttaa ttgatttcat attactattg tataatgatt tttgatttta gtttagtatt
       61 atcgtagaaa atggactcgg tgctatggat acaccggatg agaatggtta tgtagcagat
      121 gactatcgga ttacttactt agaggcccac atcaaggcca tgcgagatgc catttaccaa
      181 gatgggattg acttgcttgg ttatacgact tggagctgta tcgatccggt ttcagctggc
      241 acaggggaaa tgaataagcg ctatggcttt atctatgtag atcgagataa tgtaggaaat
      301 gggactctca aacgtagcaa gaagaagtcc ttctattggt acatgtcatt tatagcaatg
      361 gtgtaagcat cgagtagagc acatttgttt actattttta taaaatcttc tccctgcttt
      421 ataagatgta aaaaagagtt caaatggaac tctttttttg ctataatgag gggagaaaaa
      481 tcagacagga gattaagatg tcagaaccat tatttttaca atcagttatg caagaaaaaa
      541 tctggggtgg taccaagcta cgtgatgagt ttggctacga catcccaagt gaaaaagtcg
      601 gagagtactg ggctatctca gctcatctaa atggtgtttc taaagttgcc aatggtcgtt
      661 acgagggaac agatcttgct actttgtatg cggaacaccg tgaattattt ggcaatcgtc
      721 cggaacctgt atttccactt ttgaccaaga tcctcgatgc caacgactgg ctcagtgtcc
      781 aagttcaccc agacgatgct tatggactcg agcatgaagg cgaacttgga aaaacagaat
      841 gttggtacat cattgcagca gatgaaggtt cagagattat ctacggtcac aatgccaagt
      901 caaaagaaga actccgccag caaatcgagg ataagaactg ggatgcgctg ttgacaaaag
      961 tgccagttaa agcaggagat ttcttctatg taccaagcgg taccatgcac gctatcgggg
     1021 cgggtatctt gattcttgaa acccagcagt ctagcgatac cacttaccgt gtctatgact
     1081 ttgaccgtaa ggatgacaag ggcaatttac gtgaacttca ccttgaaaaa tctatcgatg
     1141 tcttgaacat tggtgaaccc gcaaatagcc gtccagtgac aatcaaagca gatgatctac
     1201 gttccactct ccttgtatcc aatgatttct tcgcagttta caaatgggaa attactggaa
     1261 aagttaactt tgaaaagaca gctgactaca gcttgttgag tgtcttggct ggtcaaggtc
     1321 aactaactgt tgacgggaaa aactatccaa tccaaaaagg gagacacttt atcctaccaa
     1381 gtgatgttga agcttggact ctggaagggc aagatttaga attaattgtt agtcatccat
     1441 aaaaaagaaa gggcctgagt tcattactca agtccttttg attacataaa ttgggcgatg
     1501 aagaccacga tgatgattgg aatgacggaa cgaagaaacg aagaaggaag agccagactt
     1561 ggaacagtcc ttgtttccat gctctttcat cgagatggag ttcctccatt gcaagagcct
     1621 ttttaaagat atagcctgta aaaagtgaaa ggaagagagc tccaaatggc atgaggagat
     1681 tggaaaccaa gaagtccata gcgtcaaaga aggtcttacc aaaaatgtga acatccgcca
     1741 tgacaccgta agatagggct gaaggaatgc caaagacaaa ggtcaaaatt tctaaaataa
     1801 cactccattt agcacgcttg ctgttgtcct gattggtgat attgtctaca ttgatttcta
     1861 gcatcacgac agaagaagtg acagtcgcaa agaggaagag gacgtagaaa atggttccaa
     1921 aaggcatctt gtcaaagagt tgaggcaaga cgataaagag caggctgggt cccccttcag
     1981 actggatatt gaagggggat cgagcttgga aaatggctag acctgccatg atggatatcg
     2041 agatattcat ggctacgatg gagattcctg actggactag attggttttc ttgtccaagt
     2101 aagaagcata ggtcaacatg actgtaaccc ctagtgagag ggcaaagaaa gattgtccca
     2161 gagcatagag gagaccagcg ctagtcagtt ttgaaaagtc tggtttgagg aagtaaagaa
     2221 ccccttccat ggcatttggc aaactgagag agcgaccgat gataaaaaca aagacgataa
     2281 agagcagggg catcatgact ttcgaagctc tttcaatctc tttttgaacc ccacgtgata
     2341 caatgaagat attcaatagg ataaaggccg cttgagctcc tagggcaatg gctggatttg
     2401 aaatgattga agtaaataac tgagcataat cacccgttcc accaagttgg aacaatttcc
     2461 caaactcaat acctagatag actagaatcc atcctccgat aacactgtaa aaagataaga
     2521 ggataaagag ggcaaaggcg ccaatccaac cgataaagtt gtacttgtta ttcttgccca
     2581 gttttccaaa ggttttgata gcggaaacgc cagcactacg gccaagggca aactcagcca
     2641 gcaggagagg gaaaccgatt aaaatagtgg aaatgagaaa gattagtaaa aagcctccac
     2701 cgccattagc agcagtcatg tagggaaact tccaaacgga accaagcccg atggccagcc
     2761 agcagatgct agaataaaac caagtttcga cccccattgt gatttttctg acatagttaa
     2821 actctgatct cctttttcaa aataagaaaa agctacttga gttgtcaaat agccttctgt
     2881 ttgattgata gacttgacta tcctatcatg ttttctaagg tctgtcaaga aaatcatttt
     2941 caagttttca cacctttctc aaaaaagtta aaaaattttc tcaaaaacgc ttgactctga
     3001 cctaagggga ggggttatac tatcattgta aggaggaaat catgtaccat ataaaagaag
     3061 ctgcgcagct ttcaggtgtc tctgtcaaga ccctgcatca ctatgacaag ataggactct
     3121 tggtcccctt aaagtcggaa aacggctatc gaacctacag tcaagaggat ttggaacgcc
     3181 ttcaggtcat tctttactac aaatatctag gcttttcttt agagaaaata gcagagctgt
     3241 taaaggaaga aaggacagat ttattgcccc atttgactag gcagttggac tatctaactc
     3301 gcgaaaggca acatctggat accttgattt ccaccttgca aaaaactatt caagaacaaa
     3361 aaggagaaag aaaaatgacc attgaggaaa aattcacggg atttagctat caagacaatc
     3421 aaaaatacca ccaagaagcg gtagagaaat atggtcaaga agtcatggga caagcgctcg
     3481 aacgccaaaa aggtcacgaa gacgaggcta cggccgcctt taaccaagtc tttcaaactt
     3541 tggcacaaaa tcttcaagtt ggtttacctg caacagcaac cgaaaaccag gagcaagcag
     3601 ccaagctctt gcaagccatt cgcacttatg gatttgactg ctctattgag gtattcggtc
     3661 atatcggtaa aggttacgtc tacaacccag agtttaagga aaacattgac aagtttggtt
     3721 ctgaaacagc ccagtacacg tcagatgcca ttgcggctta cgttcagaca aatgcagaat
     3781 aaataggcta ggaatttcct agcctatttt ttacttcaaa tcataaagcc agtcgtcacc
     3841 gtttttgtag taaaagaatt cactgagatc ttcttctaga aacacacgaa gcatatcaga
     3901 catatcatcg gttgcaagtt ttagatgaga aagattttca aagtcctccc accaaacttt
     3961 cccttcgtct gaagactgga gttcaccagt aaagtgttct gtcttgtaaa aaaggacgac
     4021 ataacgataa tccttgtcgt cataccagtt tttgatacca cagagttggg gtttggaaat
     4081 gatcagacca gtttcttctt tcacttcacg aatgacagca tcgacaaagg attcgccacg
     4141 ttcaacatga ccaccaggaa aagtaatgcc agaccagtcg ggactaactc ggtcttggac
     4201 caggacctta tctccgtttt taatcataca catgttaaca aattcgactg cctctcttct
     4261 gttcattctt cacaaccttt aatctttaat cataatgcag acttcccgcc acccagccgg
     4321 tacagagggc agaagtgatg ttaaagccac ccgtgtgggc attgatatcc ataacttcgc
     4381 ctgcaaagtg gaggccaggt accagcttac tttcaagggt tttaggattg atttccttga
     4441 gactgactcc acccttggta acaaaggact ttgcaaggga catttttcca gttacaggaa
     4501 ttttaagttc tttaatggac tggacaagtt gttctcgttc cttttcagtc agttgtttga
     4561 ctttttcagg atatccttgt acaaaaaatt cggccaagcg ttctggtaac aaggttttta
     4621 aagcgttttt caaggatttt tcccgatttt cttctagaaa tgtaaccaag tccttctcag
     4681 aaagttgagg caaaacatcg agtgagagaa cctccccacc tttgacaaag ctagacatgc
     4741 gtagggcagc aggacctgac aaaccaaagt gggtaaagag taaatcatga gtgatgacat
     4801 gcttaccata acttagggtc acctcgtcca gagaaatccc ttgtaaggct ttatgtggaa
     4861 aatctgttaa taaaggactt tcagcagcct caagatcggt gatggtatgc ttaaaatggc
     4921 gagcaatctc gtgaccaaaa ccagtcgaac cagtcgaagg ataagactta ccacctgttg
     4981 tgacaatgag tttctcacaa gtgaaggttt gatccgctga cttaaggaca aactggttat
     5041 ctactttttt aacagaaacg atttctattt gagtagcaac ttgaccacct agttcggtga
     5101 ttttcttttc caaagcttcg ataatagtcc gagacttgtc actggctgga aagacgcgtc
     5161 cgtggtcttc gaccttaagt ttaacaccat tttctgtaaa aaagttgatg atgtcatgat
     5221 tatcgaactg ggagaaaaca ctgtaaagaa agcgtccgtt tccaggaatt ccagctagca
     5281 ggttgtctaa gctaccattg ttggtcacat tgcaacgtcc cccaccagtc ccagctaatt
     5341 tttttccaag tttccgattt ttttcgatga ggagggtttt ctgtccataa aagctactgg
     5401 aaatcgtagc catcatacca gcaggtcccc caccgatgac aatagtatca aaatgtttca
     5461 tagctctatt gtaccacaaa aaaacaagag atgatggtca cctcttgtca agaatgcaat
     5521 taatcaattt catagcccat cagcaaaccg ccctcttctg catagaaact gcagagacca
     5581 gaggttggta gaattttaat atccgcttgt gggaaggttt cacggattcg ctctgagagc
     5641 tgttgacaac atttttcgtt attgcgttgg gccatgacaa tacggccacc agcatatcca
     5701 gcttttacta actcatcata ggcagcttga actgatttct ttgatcccct tgctttttgt
     5761 agcaattcga gagttccagt ttcactagct tttccgacca tacgaatgtt gagaaggcca
     5821 acgaccgtac cgataagctt gctcaaacgg ccgttcttca ccaagttatc gactttggct
     5881 aggacaaaga gcaacttagt tttttcttga taggcagtga tagcttcaac cacttcttca
     5941 aaagacaagc cctggtcaat caagtcattc aatttttcta cgagtaggtc aacttcacca
     6001 ccagcagata aactatcaat cacatgaatc ttagtgtcag gatggtcttc cagataaata
     6061 ttctttgcta gttgagcact attgtgactg ccagaaaggg tacctgtgat ggttactagg
     6121 aaaatgtttt tggcaccttc aaatgctcgc aaatagtcat ctgggcttgg acaagctgat
     6181 tttgaagctt ctgcagttgc atacatggtt tccatcattt ggtcaatatc gagactggcg
     6241 tcatcaacaa agacctgatc agctacttga atggttaagg ggacacttac aaaggttgtg
     6301 ttaatagctg gtgttggcag ttgacgataa tcacaaccag agtcagcaat aatcttccaa
     6361 gtcatagaaa ttctccatct ttgtcagtta tacattgaca aaggttctgt ctttttttac
     6421 aattatatca tgaaaactct tgaaacaaaa gcatcatccg tctgttgtga caagtagaaa
     6481 gaaaatgtta tgtctgaacg tagaatctct gaaaagtctc ttgaaaatct cagaaaatca
     6541 aaccaagaat ccaatttatt aaccagagaa gccattgaaa cagccctctt gcaactcttg
     6601 gaaaaaaagg agctgaccaa gattagtatt tctgaattgg tcaaacgtgc aggtgtttcg
     6661 cgtgcggcct tttatcgcaa ttatgactcc aaagaggaaa ttttagagag cgtctttaaa
     6721 cgaactgtcc acaatattat ggaacagctg catcattacg atttaaagac agacctttat
     6781 ttggtttggg ttcacctttt ccgggaggcc agaaaggaag ctagagtaat tcaattggcc
     6841 ttggattacc atctggaaaa aatctttgtc caagccatgc aggaatttct agaaaaatac
     6901 catgggaaat caaaaggtgt cagctcttat cttcattcct tctggagctc agccatcgtc
     6961 tctgtccttc taaaatggat caaggatggc atgaaggtac cagctgaaaa gattgcagat
     7021 ttaggtttac cattttttaa aaaatagaga aaaaggagaa aagagatgac tgaaaaaaga
     7081 ctggcctggg atgagtattt tgcagcccaa gccctactaa ttgcgaatcg ttccacttgt
     7141 aaacgtgcta aagtgggcgc gattctggta aaagataata aggttatttc cactggttac
     7201 aatggttcgg tgtcagggac tgagcattgt attgatcacg aatgtttggt cattgaaggc
     7261 cactgtgttc gcacccttca cgctgaggtc aatgctatcc ttcaaggtgc tgagcgtggt
     7321 gttcccaaag gctttacagc ctatgtaacc cattttcctt gtctcaactg tacaaaacaa
     7381 ttgcttcagg tcggctgcaa gcgcgtggtt tatatcaacc agtaccgaat ggacgactac
     7441 gcccaatacc tttatcaaga aaaagggaca gaattgaccc atttaccact tgagacagta
     7501 cagacagctc ttaaagaggc tgatctaatg taaaaattat caaaaataaa tggtttagaa
     7561 agatttttaa accgtttttt ggtataatga gaagaataaa ttgaaagaag gaattccaaa
     7621 aatgggaaaa attgaagtta ttaatcaccc actgattcaa cacaaattgt caatcttgcg
     7681 tcgtatagat acttctacaa aagcttttcg tgagctagta gatgagattg caatgttgat
     7741 ggggtatgaa gtacttcgtg atcttccact agaagatgtg gaaatcgaaa caccaattac
     7801 aaaaacagtt caaaaacaat tggcaggtaa gaaattggcc atcgtcccaa tcttgcgtgc
     7861 aggtatcggg atggttgatg gtctcttgag cttggttcca gctgctaaag ttggccacat
     7921 cggtatgtac cgtgatgaag aaacacttca accagttgaa tacttggtga aattgcctga
     7981 ggacattgac caacgtcaaa tttttgtagt agacccaatg ttggcaacag gtggctcagc
     8041 aatcttggct gttgattctc ttaaaaaacg tggcgcatca aatatcaaat ttgtctgcct
     8101 tgtatctgct ccagagggtg taaaagccct tcaagaagct catccagatg tagaaatctt
     8161 tacagcagcc ttggatgaac gtttgaacga acacggttat atcgttccag gtcttggaga
     8221 tgctggagac cgcttgttcg gtacaaaata agatcgaaaa gaagaatgac ttggaaaagt
     8281 atttccagtc acgaaaggag gttgggtttt tgtttctgtc taatgaaagc agagcaaaaa
     8341 tttgaccttt tttgaccaag atattataat agtcttatct tcagtcattt gaccaacaaa
     8401 attaaaactc aaaaggagaa atgaatgatt cctgtagtta ttgaacaaac aagccgtgga
     8461 gaacgttctt acgatattta ctcacgtctt ctcaaagacc gcatcattat gctgacaggt
     8521 ccggttgaag acaatatggc taactctgtt attgcccaat tgcttttctt ggatgcccaa
     8581 gatagtacaa aagatattta cctttatgtc aatacaccag gtggttctgt ttcagctggt
     8641 ttggcaatcg tagataccat gaactttatc aaggcagatg tccaaaccat tgttatggga
     8701 atggctgcat ctatggggac tgtcatcgca tcaagtggag caaaaggcaa acgtttcatg
     8761 cttccaaatg ctgaatacat gattcaccaa ccaatgggcg gtacaggtgg tggtacccaa
     8821 caaactgata tggctatcgc tgcagaacac ttgctcaaaa ctcgtaatac cttggaaaaa
     8881 atcttggctg aaaattcagg tcagtcaatg gaaaaagtcc atgcagatgc agaacgtgat
     8941 aactggatga gcgcccatga aacacttgaa tatggcttta ttgatgaaat tatggccaac
     9001 aattcattga actaatgatg atagaaggca aactcgactg ggtttgcttt ttttggtata
     9061 atagggagag atttcttaga aagaggattt atcatgtttg aaaaagtcaa tcgctctggc
     9121 ttgattatct atctttacta taatcgtgat gccaaaaaac tgcaggatta tggagatatt
     9181 acctatcatt ccaagaaaca tcgttactta caactctatg ttccaactca agaagtggag
     9241 caattggtcg gacgcttgag caaggaaaaa tttattaaaa aagttagggt ttgtcatatc
     9301 caagagttgg aaacaccctt tgtgggcaat ctttatcgat aggaaaacgt tatcatcgaa
     9361 aaagttcaag gaaaacattg acaattttct gataattcgg tatattctta acagactatt
     9421 taagaaataa ggagacaaaa agatgaagaa aaaatttgcc ctatcgtttg tggcgcttgc
     9481 aagtgtagca cttcttgcag cctgtggaga agtgaagtct ggagcagtca acactgctgg
     9541 taactcagta gaggaaaaga caattaaaat cgggtttaac tttgaagaat caggttcttt
     9601 agctgcatac ggaacagctg aacaaaaagg tgcccaattg gctgttgatg aaatcaatgc
     9661 cgcaggtggt atcgatggaa aacaaatcga agtagtcgat aaagataata agtctgaaac
     9721 agctgaggct gcttcagtta caactaacct tgtaacccaa tctaaagtat cagcagtcgt
     9781 aggacctgcg acatctggtg cgactgcagc tgcggtagcg aacgctacaa aagcaggtgt
     9841 tccattgatc tcaccaagtg cgactcaaga tggattgact aaaggtcaag attacctctt
     9901 tattggaact ttccaagata gcttccaagg aaaaattatc tcaaactatg tttctgaaaa
     9961 attaaatgct aagaaagttg ttctttacac tgacaatgcc agtgactatg ctaaagggat
    10021 tgcaaaatct ttccgcgagt catacaaggg tgaaatcgtt gcagatgaaa ctttcgtagc
    10081 aggtgacaca gacttccaag cagcccttac aaaaatgaaa gggaaagact ttgatgctat
    10141 cgttgttcct ggttactata atgaggctgg taagattgta aaccaagcgc gtggcatggg
    10201 aattgacaaa ccaatcgttg gtggtgatgg attcaacggt gaggagtttg tacaacaagc
    10261 aactgctgaa aaagcatcaa acatctactt tatctcaggc ttctcaacta ctgtagaagt
    10321 ttcagctaaa gctaaagcct tccttgacgc ttaccgtgct aagtacaatg aagagccttc
    10381 aacatttgca gccttggctt atgattcagt tcaccttgta gcaaacgcag caaaaggtgc
    10441 taaaaattca ggtgaaatca aggataacct tgctaaaaca aaagattttg aaggtgtaac
    10501 tggtcaaaca agcttcgatg cagaccacaa cacagtcaaa actgcttaca tgatgaccat
    10561 gaacaatggt aaagttgaag tagcagaagt tgtaaaacca taatagaaaa atgttgaaat
    10621 agggaatgag cctttgactc actccctgtt tcgatattta atactcttcg aaaatctctt
    10681 caaact
//
LOCUS       DAVBNO010000064        10517 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVBNO010000064 DAVBNO010000000
VERSION     DAVBNO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.064"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..183
                     /locus_tag="VQ846_001793"
     CDS             <1..183
                     /locus_tag="VQ846_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3076328.1"
                     /translation="LTVEDKAYNHALSKERSKVENIFVKVKTFKMFSTTYRNHRKRFG
                     LRMNLIAGIINHELGF"
     gene            251..499
                     /locus_tag="VQ846_001794"
     CDS             251..499
                     /locus_tag="VQ846_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076329.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            630..1802
                     /locus_tag="VQ846_001795"
     CDS             630..1802
                     /locus_tag="VQ846_001795"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET3076330.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTVLSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1802..3076
                     /locus_tag="VQ846_001796"
     CDS             1802..3076
                     /locus_tag="VQ846_001796"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET3076331.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(3111..4076)
                     /locus_tag="VQ846_001797"
     CDS             complement(3111..4076)
                     /locus_tag="VQ846_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET3076332.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     SYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILIIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(4057..5511)
                     /locus_tag="VQ846_001798"
     CDS             complement(4057..5511)
                     /locus_tag="VQ846_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET3076333.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLIFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDCFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            5887..7770
                     /locus_tag="VQ846_001799"
     CDS             5887..7770
                     /locus_tag="VQ846_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET3076334.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGDKVKVGDVLGTFDSNKIATAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            7919..8806
                     /locus_tag="VQ846_001800"
     CDS             7919..8806
                     /locus_tag="VQ846_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET3076335.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(8849..9466)
                     /locus_tag="VQ846_001801"
     CDS             complement(8849..9466)
                     /locus_tag="VQ846_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET3076336.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(9881..10069)
                     /locus_tag="VQ846_001802"
                     /pseudo
     CDS             complement(9881..10069)
                     /locus_tag="VQ846_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3059 a         2055 c         2337 g         3066 t
ORIGIN      
        1 ctaacagttg aagataaagc ctataaccat gcgctatcta aggagagaag caaggttgag
       61 aacatctttg tcaaagtaaa aacgtttaaa atgttttcaa caacctatcg aaatcatcgt
      121 aaacgcttcg gattacgaat gaatttgatt gctggcatta tcaatcatga actaggattc
      181 tagttttgca ggaagtctac tactttcctt attgtctgta aatctactga ccttgttgtt
      241 tatcccagtc atggtttcta gttcgggctc agagtttcaa agtggatggc aagagcatca
      301 attgattgct gagaaggtta gtaaaacact tgataagaca tttgataagg atgtcagaga
      361 aattccgacc agtcagtttt atcaaaaatt tgtagatgag atgggaagga cttactcagg
      421 aaatttgatc ctccaggagc tgataactgt gaatggagct tataaagcta cttatatcgg
      481 tgaactctct agcaactaat cattctttaa aatgatgacc aataagaggc ttgatttggt
      541 gaatcaagtc ttttttctct cttttactgc catttgtgat aaaatagtat agaacgattt
      601 tttacatgaa tgataaaaca gaggtaaata tgacaatcgg tattgataag attggttttg
      661 cgaccagtca atatgtcttg aaattacaag acttagcaga agcgagggga attgaccctg
      721 aaaaattaag taaaggactc ttactcaagg aattgagtat tgcgccccta actgaggaca
      781 tcgtgacctt ggcggccagt gctagtgact ctattttaac tgagcaagaa agacaagaag
      841 ttgacatggt cattgtggcg accgagtcag gaattgacca gagtaaggct gcggccgtct
      901 ttgtgcatgg cttgctgggc atccagccct ttgctcgtag tttcgagatt aaagaagcct
      961 gctatggggc gactgctgcc ctccattatg ccaaattgca tgtggaaaat tctccggagt
     1021 ccaaggtctt ggtcattgca agtgatattg ccaaatacgg tattgaaact ccgggagaac
     1081 ccactcaggg tgccggaagt gtggctatgt tgattacaca aaatccacgc atgatggcct
     1141 ttaataatga caatgtagct cagacccgtg acatcatgga tttctggcga ccaaattact
     1201 cgacaactcc ttatgtaaat ggtgtctatt ctacccaaca atacttggat agtttgaaaa
     1261 cgacttggct tgaatatcaa aaacgctacc agcttacttt ggatgatttt gcggctgttt
     1321 gtttccactt gccttatcct aaattagcgc taaaaggctt gaaaaaaatc atggataaga
     1381 gcctgcctca agagaaaaaa gacctcttac aaaagcattt tgaccagtct attctctaca
     1441 gtcaaaaggt ggggaatatc tacacaggtt cacttttcct tggacttttg tctctcttgg
     1501 aaaatacaga tagcttgaaa gctggggata aaatcgccct ttatagttac ggaagtggag
     1561 ctgtggctga gttcttcagt ggtgaattgg ttgaaggata tgaagcttat ttggataaag
     1621 accgcttgaa caagctcaac caacgaactg tcttatccgt tgcagactat gaaaaggtct
     1681 tttttgagga agtaaacttg gatgaaacaa actctgccca gtttgctggc tatgaaaatc
     1741 aagattttgc cttggttgaa attctcgacc accaacgccg ttatagcaag gttgaaaaat
     1801 aatgaagata agttggaatg gattttctaa aaaatcatac caagagcgcc tcgagctgtt
     1861 aaaagctcag gcgctcctta gtcctgagag acaagctagt ctggagaagg atgaacagat
     1921 gagcgtgact gtggcagacc agctgagtga gaatgtagtg ggaacttttt ctctgcctta
     1981 ttcactggtt ccggaggtac ttgtcaacgg tcaggaatac accgttccct atgtgacaga
     2041 agaaccctct gtggttgcgg cggccagcta tgccagcaaa atcatcaagc gtgcaggtgg
     2101 ttttactgca caagtccatc agcgacagat gattgggcag gtagcccttt atcaagttgc
     2161 taatcctaaa ctagcgcaag agaagattgc cagcaagaaa gcggagctct tggagcttgc
     2221 caatcaagcc tatccttcta tcgttaaacg tggaggtggg gcgcgtgatc tgcatgtcga
     2281 gcagataaaa ggcgaaccag actttctcgt tgtttatatt catgtcgata cccaggaagc
     2341 catgggtgcc aatatgctca acaccatgct ggaagccttg aaaccagtct tagaagaact
     2401 cagtcaggga cagagtctca tgggaatcct gtccaactac gcgaccgatt ctctggtgac
     2461 tgcaagctgt cgcatcgcct ttcgctactt gagccgccaa aaggatcaag gacgagagat
     2521 tgcggagaaa attgcgttgg ctagtcagtt tgcgcaggct gatccttacc gagctgctac
     2581 tcataataaa ggaattttta atggtattga tgcgattttg attgccactg gtaatgactg
     2641 gcgtgccatc gaagctgggg cccatgcctt tgccagtcga gatggacgct atcaaggtct
     2701 tagttgctgg acgctggacc ttgaaagaga agaattggtc ggtgagatga ccctgcccat
     2761 gcctgtagcg actaagggtg gctctatcgg cctcaaccca cgtgtagctc tcagtcatga
     2821 tctactagga aatccttctg ccagagaatt agcccagatt atcgtgtcca tcggtcttgc
     2881 ccaaaatttt gcagccctca aagccttggt aagtacgggc atccagcaag gccacatgaa
     2941 actacaggcc aaatccctag ctctcctagc tggggctagt gaatctgaag ttgctcccct
     3001 agtagagcgc ctcatctcag ataaaacctt taacctagag acagcccagc gctatctcga
     3061 aaatttaaga tcataaaaac tcagacgaat cggtctgagt tttcttgtgt ttaaatactt
     3121 tttcctggta atagtgtaac tggtaagaag taaccagttg tggcgacttc cttgccttca
     3181 atcttttgca agagaagatc aatagtgaga caagcaatct cttccaaagg ttgcttgata
     3241 gtagccaatt gagggtagta attttcgata aagtaggtcc catcatagcc gataaccttg
     3301 agctcttttg ggacagaaat gcccaattct tgagcgattt taatgatcag aatagctgtc
     3361 aaatcatccg aagcaaaaat ggcatctggt ttttcccggg tcaagatatt cttgatttcc
     3421 atttcttttc tgacgggaga aaagtcactg gaaacattga taataggagc ttttgggagt
     3481 acggatgcaa aaccagcgtg gcgcagtccg gttggcgaat tggaattgtc attccctgta
     3541 atcatgatga tagactgggc acctgtcttg accaaggttt gggcagcaag aaccccacca
     3601 gcatagttgt cagaggagac aacagggatg tctggcgata ggtttcggtc aaaggaaata
     3661 atcggcgctg tcacacgatt gtagtcttcg attcctaggt tgtgactacc agaaatgatg
     3721 ccgtctacct gattggcttc caacatttcg atgtattcgc gttccttctc agaatcatgt
     3781 tcactgttgc agatgatggt cttgtaacca tttttgaaga gttggtgttc caatttatca
     3841 atcaattctg catagaaaac attggaaata ttggggaaaa tcaagccgat taacttagct
     3901 gattttcctt gcagactacg agccaggttg ttgggtttat agcttaattc tctcatggct
     3961 tcattgactt tttggatggt tttctcagat agataccctt ttttattgat aacccgagaa
     4021 acggtagtag gactgacgcc tgcaagtttg gcgacatcag ttagttttgc gaccataatc
     4081 taattcatag taagttccag ttgggtttcc agatttgatc aggataccat tttggtccgc
     4141 atgtgggaag acacgaccag aaaatacttt ttctccttta ttgatgaaaa tttcaaagac
     4201 agagttatcg atgaagattg tagcagtagt agcctgattc tcgatagggc aagaacgagt
     4261 tgtcccaaat tcttgggcat actgttctcc agcctggcta cgatccactg tcacttgacc
     4321 gtttacaagg tcaaagttga ttgaaagtcc cttaccttct ttatcagcaa gtaagacaat
     4381 ctcgctctgg ctattagctt ccaagttgag ttcaagttcg taagtgttct tggtttggga
     4441 acggtttgag aaggcttctt cagaagcacg aaggtcctta atagcagcga ctggatactg
     4501 gtagagcttg tcgtctttga tagtgagttc cttgaccaaa gagaaggttc cttggtgatc
     4561 aaaacagtca gatgggtaag aaacatctgg caaaccaagc cagctaactg ctagagcacg
     4621 cccatcagga gcgttgaagg cttgagttgc ataggcttcg aaaccgtaat ccatgttttg
     4681 aagttgagac acatctacca ttttggcatt tttagggtca aaggaagccc caatcttata
     4741 catatttgga aagatattat cgtagtctag aactttctta tccaatcctt gtggacagta
     4801 gagaaggaca ggctgttcct ctacaaagat cagattagga cattccatca tgtaggcagt
     4861 acggtcgtta gcaaagtcaa ggtcgccaac tgcttgccag tttgtgtagt cgttattgac
     4921 agccttgtag agacggacga agcctttttt ctccaagtcc tgtccaccga cgacagcata
     4981 gtattgacct ttaaagttaa aaatttgtgg atcgcggaag tggtcagtag agtctgctgg
     5041 ctggtcaatc aagatcttgt caatctttgt aatcttaccc tccttgtcca tcaaagcacc
     5101 gatctggtat gggtgacgga tccagttttc atcgcgaaca tttcctgtat aaaataggaa
     5161 taagttatcg ccaaattgca tggcagaacc agagtaggca ccgtggctat ctaatggagt
     5221 atctggtaaa actttgattc cagtttcttt aaagtgaacc aaatcatcac tttctagctg
     5281 tgcccaagat ttcaaaccgt gggctgcacc aaaaggaaaa ttctggtaaa agaggatcca
     5341 cttgccatca aagtaagaaa agccatttgg gtcgttgaga agtcctgttt ttggctcaac
     5401 atggtaatga gtatgccatg gagattgtgc catattttcc tttatatgtt gaatttcttc
     5461 ttgcgtccaa tcttgataaa gtctgtaacg acgctcagtt gtccattcca ttcattcctc
     5521 caaaaatctt atcactttta atagtaactg ttttcgataa aaaatgcaag ccttttatgt
     5581 taaaaaagag gaaaaactgt caaacgatta tcataaaata aatacaggaa atcaatcaaa
     5641 tttttacgaa aatgtgaaag ataatgaaat aaaatacttt ttaaccaagg tttcaaactt
     5701 atttttcaag gaattcctga tgaaacagtc aaaaacaatc aatctaaagc ttgcccacta
     5761 agtggataga atatttttat gcaaaacgct tgacatattt ttaaaacatg ataaaataat
     5821 agtcgtaagg gcgaataaaa tcgctttcaa acaagaacaa aatgttatat aaggagattt
     5881 ttgcaaatga acaatcagga aattgcaaaa aaagtcatcg atgccttggg cggacgtgaa
     5941 aatgtcaata gtgttgccca ctgtgcgact cgtctacgtg tcatggtcaa agatgaagag
     6001 aaaatcaata aagaagtgat tgagaacttg gaaaaagttc aaggtgcttt ctttaactca
     6061 gggcaatacc aaattatctt tggtacaggt acagttaaca aaatgtacga tgaagttgtt
     6121 gtacttggat taccaacatc atctaaggat gacatgaaag cagaagttgc taaacaaggg
     6181 aactggttcc aacgtgctat ccgtactttt ggtgatgttt tcgttccaat catcccagtt
     6241 atcgtagcga caggtctctt catgggtgtg cgtggtcttt tcaacgctct tgaaatgcca
     6301 cttccaggtg actttgcaac ttacacacaa atcttgacag atacagcctt catcatcttg
     6361 ccaggtttgg ttgtgtggtc aaccttccgt gtatttggtg gaaatcctgc cgttggtatc
     6421 gttcttggta tgatgcttgt ctctggctca cttccaaacg cttgggcagt tgctcaaggt
     6481 ggtgaagtaa cagcgatgaa cttttttggt ttcatccctg ttgttggttt gcaaggttcc
     6541 gttcttccag ccttcatcat cggggttgtc ggagctaaat ttgaaaaagc tgtccgcaag
     6601 gttgttccag atgtcattga cctcttggta acaccattcg tgacactttt ggtcatgtct
     6661 atccttggac tctttgtcat tggaccagtt ttccacgttg ttgaaaacta catccttatt
     6721 gctacaaaag cgattcttag catgccattt ggtcttggtg gtttcttgat tggtggggtt
     6781 caccaattga tcgtcgtgtc aggtgtgcac cacatcttca acttgcttga agtgcaatta
     6841 cttgctgctg accatgctaa cccattcaac gctatcatca cagctgctat gacagctcaa
     6901 ggtgctgcta ctgttgcggt tggtgttaaa acaaaaaatc caaaactgaa aacacttgct
     6961 ttcccggctg ctctttctgc cttcctaggt attacagagc ctgctatctt cggggtgaac
     7021 ttgcgcttcc gtaaaccatt cttcctttca ttgattgctg gtgcaatcgg tggtggattg
     7081 gcttctatcc ttggacttgc tggtactggt aatggtatca ccatcatccc tggtacaatg
     7141 ctttatgttg gtaacggaca acttccacaa taccttctta tggtagctgt atcatttgcc
     7201 cttggttttg ctcttactta catgtttggt tacgaagatg aagtagacgc aactgcagct
     7261 gcaaaacaag ctgaagtggc tgaagaaaaa gaagaagttg cgccagcagc tcttcaaaat
     7321 gaaacacttg taactcctat cgtcggtgat gttgtcgctc ttgctgatgt caatgaccca
     7381 gtcttctcaa gtggagctat gggacaaggt atcgctgtga aaccaagcca aggcgtggtc
     7441 tatgcaccag ctgatgctga agtttcaatt gcctttccaa cagggcacgc ttttggtttg
     7501 aaaacaagaa atggtgctga agttttgatt catgttggta ttgacactgt atctatgaac
     7561 ggtgacggtt ttgaagcaaa agttgctcaa ggtgataagg tgaaagttgg cgatgttctt
     7621 ggaacatttg actcaaataa aatcgctaca gctggacttg atgatacaac aatggttatc
     7681 gttacaaata cagctgacta cgcttcagta gctccagtcg caacaggttc agttgcgaag
     7741 ggggatgctg tgatcgaagt gaaaatctaa tcaatcctct ctaatgtgaa aacgaacaaa
     7801 tgacatgttt gttcgttttt attagatagt aagatttaca gaggaaaatc taaaaaatag
     7861 agacatttag actttcgaag tatgctataa taaagaaaat aaaaacaaga ggtttatcat
     7921 gacaaaatta tatggaagct tggaagctgg cggtacaaag tttgtctgtg ctgttggtga
     7981 tgaaaacttt aacgttgtag aaaaaacaca atttccaaca acaactccaa tcgaaacaat
     8041 cgataaaacc attgagttct tctcaaaatt cgataacctt gctggtcttg cagttggttc
     8101 atttgggccg attgatattg acaaaaactc aaaaacttat ggctttatca cgacgactcc
     8161 aaaaccaaac tgggcaaatg tggacttgct tggtgccctt cgtcgcgccc taaacgtgcc
     8221 aatgtacttc acaacagacg taaacagctc tgcttacggt gaagtggttg cccgtaacaa
     8281 tgctggtggt cgtatcgaaa acttggttta ctacacaatc ggtacaggta tcggtgcagg
     8341 cgtcatccaa cgtggtgagt ttatcggtgg tgtgggtcac cctgaaatgg gtcattatta
     8401 tgttgctaga cacccaatgg atattgaaaa agagtttaag ggtgtttgtc ctttccataa
     8461 gggatgtctg gaaggctatg cagctggtcc aagtttggaa gctcgtacag gtgtacgtgg
     8521 ggaaactatt gaactcaaca accctgtttg ggatgttcaa gcctactata tcgctcaagc
     8581 tgcggttaat gcgacagtga ctttccgccc agacgtgatt gtctttggtg gaggggtcat
     8641 ggctcaacaa catatgctgg accgtgtccg tgagaaattt acatctcttc ttaatggtta
     8701 cctaccagta ccagatgtgc gtgactatat cgtgacgcca gcagtcgcag gaaatggttc
     8761 tgccacactt gggaactttg ttcttgcaaa agaagtttca aaataaagca caaaatccgc
     8821 ttgggaaacc aaacggattt tttacgtgtc aaagcatttg ccagaaaaag tcaataatat
     8881 aggtcagacc gtaggtataa accacgaggg taaagggttt ggtcagaatg atgatggtca
     8941 tgtagcgctt gaaactcatc ttggtcaggg cagccagcat acagagaaag tcagctgggc
     9001 taatgggcca aatcatcata aaaataaaga agcggtcaaa acgattgccc ttatctagcc
     9061 agccgatgta cttgtcgtag gtgcgcttgc tgacgacaga ctggacaaag gcagctccgt
     9121 ataggcgcac tagataaaag ataatggcac agccaatcac gatgccgata tagttgtaga
     9181 tagtcccgat gatgtgcccg tagataaaga ccccagccac cgaggtcaag gcccctggaa
     9241 tgatagggac gacagtctgt aaaatctgta aaaagataaa gagaggtgga ccccagatgc
     9301 ctgcctgctg gataaaggca gagagggttt ccttggattg taaaatccca gcctgataag
     9361 cccaaataca gaaaatcaaa ctcccaaccc caccgacaat cgatgagata ttgataactc
     9421 gctgcaaaag gggagaaaca gctttcattt gtttatcctg tgacatgtaa actcctcata
     9481 gatagtatat ttctactata ccatattttg gaggagaaag ggggaatgag tcttttttga
     9541 taaggaatag aaagtaaata gcccttcgag acttagaata ggtctgtcaa ctaaaggtta
     9601 tatgcattct aggtttcaaa taatcgcaat tccataaatt taaaagcatt ctaagtttcg
     9661 aatagcttat ctgagataat tttgaggcat tcgcgctttc gaatagtcat tttccctaaa
     9721 gtttagatgt attctgaagc tagaaagact aaaatctaaa agtagtaaga gtctctctct
     9781 gatggacgta atttatggct agtatgaatc ctttgaattg ttagttaatt ttaatattaa
     9841 tcagttctga aataattact aaatatagtt agatggtgaa ttaattattg ctaaatttca
     9901 agttcaagtt agtcatccag aagtcttctc tgggggactt gtagttcaag cattttttag
     9961 gatagttgtt aatccacttt tcgatgaatg cgacttcctt tgtagtcgtt ttcttagttt
    10021 cctgaggtaa ctatctatga atgagcctgt tgtgattttc attagatcct ataggggtgt
    10081 gcataataga tatagcagat tgaaactaga atagtataca tctacttcta aaacattgtt
    10141 agaaatcaat ttgactgtcc tgatcaattt gtcctgttct tatttcattt tactatataa
    10201 tccttagaaa acatatggga caagcggttg aattctgttc cgtccattag tagataaagg
    10261 attactcctt cttgaaatga cagatgaact tgtttcttat aaaaatgttg tctgaaacta
    10321 ttagcctttt tccagaagtt ttagaggaag aaaactttac gaggaaaaag gagttactca
    10381 aataacaatt caggattaaa aatagacagt tgaggagcgg aaggtataaa ttaagtttgc
    10441 tgctgtataa tggatttatc actaatactc ttcgaaaatc tcttcaaacc acgtcagctt
    10501 cgccttgccg tagatat
//
LOCUS       DAVBNO010000065        10507 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVBNO010000065 DAVBNO010000000
VERSION     DAVBNO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.065"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            168..380
                     /locus_tag="VQ846_001803"
                     /pseudo
     CDS             168..380
                     /locus_tag="VQ846_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            280..726
                     /locus_tag="VQ846_001804"
     CDS             280..726
                     /locus_tag="VQ846_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET3076337.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(864..2168)
                     /gene="eno"
                     /locus_tag="VQ846_001805"
     CDS             complement(864..2168)
                     /gene="eno"
                     /locus_tag="VQ846_001805"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET3076338.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2332..2778
                     /locus_tag="VQ846_001806"
     CDS             2332..2778
                     /locus_tag="VQ846_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET3076339.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPLGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(2775..3890)
                     /locus_tag="VQ846_001807"
     CDS             complement(2775..3890)
                     /locus_tag="VQ846_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET3076340.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPSGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASDQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(4004..4228)
                     /locus_tag="VQ846_001808"
     CDS             complement(4004..4228)
                     /locus_tag="VQ846_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET3076341.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAE"
     gene            complement(4173..4544)
                     /locus_tag="VQ846_001809"
     CDS             complement(4173..4544)
                     /locus_tag="VQ846_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET3076342.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4603..6036)
                     /gene="glgA"
                     /locus_tag="VQ846_001810"
     CDS             complement(4603..6036)
                     /gene="glgA"
                     /locus_tag="VQ846_001810"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET3076343.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGSQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6033..7172)
                     /gene="glgD"
                     /locus_tag="VQ846_001811"
     CDS             complement(6033..7172)
                     /gene="glgD"
                     /locus_tag="VQ846_001811"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET3076344.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7162..8304)
                     /locus_tag="VQ846_001812"
     CDS             complement(7162..8304)
                     /locus_tag="VQ846_001812"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET3076345.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8294..10222)
                     /gene="glgB"
                     /locus_tag="VQ846_001813"
     CDS             complement(8294..10222)
                     /gene="glgB"
                     /locus_tag="VQ846_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET3076346.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGSLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFASQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3199 a         2302 c         1848 g         3158 t
ORIGIN      
        1 cgactttttt gaagcttggt ttcagaaatt tctcttacca acattaaccg caccattggt
       61 tattatatta aaacgaagta agaataggac aaatcgatca ggacagtaaa atctaattct
      121 aacaatgttt tagaagtaga ggtgtactat tctagtttca atctactata ttaatggaca
      181 atgcaagatt ccatagaatg gggaagctag aactcttgtg tgaagagttt gggcataaac
      241 ttttacctct tcctccctac tcgcctgagt acaatcttat tgagaaaaca tgggctcata
      301 tcaaaaagca cctcaaaagg gtattaccaa gttgcaatac cttttatgag gctcttttgt
      361 cttgttcttg tttcaattga ctatataaat tgtctaagcg aaacaaccga taagaattgg
      421 cacaaaagcg accgtatttt tgttaccaat acaggaaaac cagttcatag ttctatcttg
      481 agcaagtctc tccagcgagc aaacgaacgc cttaaaaaac caattcccaa acatctgtcc
      541 cctcacatct tcagacacac cactattagc atcttatcag aaaataaaat tcctttaaaa
      601 acaatcacgg acagggttgg tcattccgac tctgaagtca ctacttccat ctacacccac
      661 gtcacaaaga acatgaaaga tgaagcaatc aatgtactgg ataaagttat gaaaaagatt
      721 ttttaaaaag ttttgtccct tttttgtcct ctaaatacaa aaatagccct tcggataaaa
      781 tccgaggggc tagaaacgtt gttaaatcaa cggccgaact tttgaatttc atggttcggg
      841 ataaaatagt tcactgaact attttatttt ttaaggttgt agaatgattt caatccacga
      901 tattcagcta cttcaccaag ttggtcttcg atacgaagca attggttgta tttagcgatg
      961 cggtctgtac gtgaaagtga accagtcttg atttgtcctg cgttagttgc aactgcaata
     1021 tcagcgattg ttgaatcttc agtttcacct gaacggtgtg atacaacagc agtgtaacca
     1081 gcttctttag ccatttcgat agcttcaaaa gtttcagtaa gagtaccgat ttggttaact
     1141 ttgataagga ttgagttagc agcaccttct tggataccac gtgcaaggta gtcagtgttt
     1201 gttacgaaga agtcgtcacc aacaagttgt actttcttac caagacgttc agtaagagct
     1261 ttccaaccat cccagtcgtt ttcatccata ccatcttcaa tagtgatgat tgggtatttg
     1321 ttaaccaatt cttcaaggta gtcgatttgt tctgcagatg tacgaacagc agcaccttca
     1381 ccttcaaatt tagtgtagtc gtaaacttta cgttctttat cgtagaattc tgatgaagca
     1441 cagtcaaatc cgataaatac gtctttacct ggtacatatc cagcagcttc aatcgcagca
     1501 aggatagttt caacaccatc ttcagttcct tcgaaacgag gagcgaatcc accttcgtca
     1561 cctacggcag tttccaaacc acgtgattta aggattttct taagagcgtg gaagatttca
     1621 gcaccgtaac gaagggcttc tttaaatgtt ggcgcaccaa ctggcaagat catgaactct
     1681 tggaaagcga ttggagcgtc agagtgagaa ccaccgttga tgatgttcat cattggagtt
     1741 ggaagaactt tagtgttgaa tccaccaagg tagctgtaaa gtgggatttc aaggtagtca
     1801 gcagcagcac gagctacagc gatagacaca ccgaggattg cattcgcacc caatttacct
     1861 ttgttaggag taccgtcaag tgcgatcata gcacggtcaa tagcttgttg atcacgtaca
     1921 tcgtagccaa tgatagcttc agcaatgatg ttgtttacgt tgtcaacagc tttttgtgta
     1981 ccaagaccac cgtaacgaga tttgtcaccg tcgcgaagtt caactgcttc gtgttcacca
     2041 gtagaagctc ctgatggaac cataccacgt ccgaaagcac ctgattcagt gtaaacttct
     2101 acttcaagtg ttgggttacc gcgtgagtct aggacttcgc gagcgtaaac atcagtaata
     2161 attgacattt tttactctcc ttatgagtta aattttttac acctctataa taccttaaaa
     2221 cccctccttt ttcaagaaaa aacgttatct ttgtgcaact tttccttaac tttataaagt
     2281 aatcgctttc ttttgtctgt tttattctaa cttttatgat atactgtttt catgacagat
     2341 ttatcaaaac aattacttga aaaagctcat ggtgggttaa aaataaatcc ggatgagcaa
     2401 agacgctatc ttggtacttt tgaggaaaga gttcttggat atgtagatat tgacacagca
     2461 aatagccctc agttagaaaa aggcttttta tttattttag aaaaccttca ggaaaaagca
     2521 gagccactat ttgtgaagat ttcaccaact atcgaatttg ataagcaagt tttctactta
     2581 aaagaagcaa aagaaactga tagtcaagcc accatagtat ctgaagagca tattacttct
     2641 cctttgggcc tggttattca tagcaatgca ccagttcaag tagaagaaaa agaccttcga
     2701 cttgcttttc caaaactttg ggaagttaaa aaggaagaac cagccaaaac atccttatgg
     2761 aagaaatggt ttagctaaat cttgcacata tttaataagt gcccaatatt ggcagccgtg
     2821 cgctccagat agagactggc atttttcaaa ctatcttcta aaggttcact tttctccaaa
     2881 atagaaaaga cagcttggat attttcaaat ggtaggggag gtaaatcttc agcaagacta
     2941 ccgcaaatag caataacagg aactccaaca ggggttcttt ttgcaacacc tataggcgct
     3001 ttcccagcaa agctttgact atcaagtctt ccttctccaa caacaaccaa gtcagcatct
     3061 gaaactttct tatcaaagtt gattaagtcc aagcaggtat caattccaga cacgatactt
     3121 gcctgagcaa aggcacacaa accaccagca aggcctccac ctgctcctgc tcctttaatt
     3181 tctaatgttg caggtgagaa tttttcataa aaatcttgga tcgcctgatc tacgactgca
     3241 aacatagtcg gatgtagacc tttttgattg ccaaaagtgt aagtcgcacc ttgatgacca
     3301 cataagggac tcacgacatc tgctagaata tgaatttgaa caccttcagg aattttatag
     3361 caattttctg ttgaaacaga agctaagttt aataaggatt gatcggaagc aggcaagaca
     3421 tttccatccc tatcataaaa ttgataacct aaaccagcag caatccccag tcctccatca
     3481 ttactggccg tgccaccaac accgatataa atatctttaa tccctttaga aatgagatgg
     3541 agaatcaact ctccaatacc acaagtttgg atttgaagtg gatttcgttt ctctagcgga
     3601 atttttccaa gaccaaccaa gtcagctact tcaaatagtg ccagttcccc tttttgaaaa
     3661 tagcgcatgg cttctttttg tccagaaggg tctgtcactt ggatccattt ttcttttagg
     3721 tcaagagaat gtcggatagc atctacagta ccttctcccc catcaccaac agggcagagg
     3781 agacattcta catctgctat cgattgttgg aagcctcttt ttattgcttc agctacctgt
     3841 tgagctgtca agctttcctt aaacgaatcc ggtgcaatta caatcttcat attttccctc
     3901 attctaaaca gtcaatcaaa ggaagaactt ctaaaaaatc cctcttgtca acatgatgtg
     3961 gtatttcttt tttgagcatt tctttggaac aaaaggcgat tcctcactcc gccgacttca
     4021 acattaatag attattgacc ccatcaccga ttgccaccgt tctttcttta gaaagtttta
     4081 gtttctttct ccatttttcc agagtttctt ttttgacctg gggacttata atttgtccaa
     4141 ctaattttcc tgttaaaaga ccttctttga cttcaagctg gttggcagtg aaataggcaa
     4201 taccaaggga ttttgctaat ctctccaact attggtgtaa atccaccaga caccagacca
     4261 actaggatgc cattcttttg gagaatagag atgaactctg ggacatttag cgatagatga
     4321 attgagttga agacgttatc aaagaccaaa ataggaagac cttccaacaa ggacactctt
     4381 tttcttaaac tgctttcaaa gaccaactct cctcgcattg ctcgacttgt aatctgcgaa
     4441 atttccgcct catgacctgc ctctctccct aaaagatcaa tcacttcttc taggattaag
     4501 gttccatcta catccaaaac acacaagcct tttacttgag acatcagttc tcctctctaa
     4561 acagcctaaa aatcgtatga agtcatcata cgattttatc tattaattaa ctaaactatg
     4621 gtacaagtca aggtatgact tgcaggctgt atcccatgag aagtcactct ccatagcttg
     4681 tttttgtagg tttctccaaa tgtctggatg gtttctatac aagtccaatg ctgtttggaa
     4741 agtccaattt aaccaataag gagatagatt gtcaaagcta aagccagtac cgcttccttc
     4801 gattggattg aaagcgcgaa ctgtatctcg caagcctcca acttcatgga ccaatggcaa
     4861 ggttccataa cgcatagcca tcatttgaga caagccacac ggttcaaaac gacttggcat
     4921 gaggaagagg tcacaagcag cgtagatttc ctgagcaagt ttgacatcaa aagtgatatt
     4981 tgttgatagc ttgtctgggt aaatctgagc aaaccatgag aaagctcctt caaaggctgg
     5041 atcgccagtt cccaaaagaa caatctgaac atcttcttgc aagatatggt gaagactttc
     5101 gaccaccaca tcaaaacctt tttgacgtgt caaacgagaa acaattccca ccagtggaac
     5161 gtctgctcta acaggcaagc caactctttc ttgcaatttt gccttatttt tggctttccc
     5221 agacaaatct tcctgattga aatgatagtc taaaagagca tccgtctgag gattataaag
     5281 atcagcatca atcccattca cgataccaga tactttacca gactccattt taagaatctg
     5341 atccaaatta catccaaact gactagtcat aatttcatga gcatagctag gtgaaacagt
     5401 tgaaacacgg ttcgcataga gaatacctgc cttcatccag ttcagacagt tgttccatcg
     5461 aagggtgcca tcagcgtaac gttcaaagcc aactccaaac aaatcaccca acattccttc
     5521 tgaaaattgt ccttggaatt ctaaattatg aatggttaaa actgtttcaa tgtcctcata
     5581 ggcttgaatc caacggtatt tttccttcaa caagaaagga atcatagctg tatggtagtc
     5641 atgaacatgg agaagatcag gaataaagtc aatcctttcc atagcctcaa tggcagccag
     5701 ttggaaaaag gcaaagcgtt ctccgtcatc aaaatcaccg taaacatgac cacggaagaa
     5761 ataatattga ttgtcaataa agtagaaggt tacaccattt aatactgttt tcttaattcc
     5821 acaatactgt ctgcgccaac caacgctcac ctcaaaatga agcacatctt caatctgact
     5881 tccaaattta gcctctacca tatcatagta gggtaaaatc actgcaactt cgtgcccagc
     5941 ttttaccagt gattttggaa gagcgccaat gacgtctccc aaaccacctg tttttgaaaa
     6001 gggtgcaccc tctgctgcta caaataaaat tttcatgaat gaatatcctc tgttacttta
     6061 gcacctttct taaccacaac tggatgttct gcagttcctc gaatcacaac accaggctca
     6121 acttcaaccc ctttgtccaa gatagcatat tcgacctgag ccccttctcc aataacaaca
     6181 cgagggaaga gcaggctatc tttaaccaag ctatccttat ggacatgaat attacgtgat
     6241 agaacagaat tagctacttg accttcaata atactaccag aggcaaactg agaagtgctt
     6301 accttagatg tattagcata gtaagttggc tcttcgtttt tgacctttgt ataaatcttt
     6361 tggtttggtg agaaaagaga atagaatttt tgtgattcaa gcatatcgat attcgcttga
     6421 taataagatt taacagagtg aatattggct agatagcccg tgtactcgta ggcgaaagct
     6481 ccctctttta cagccaaatc ccgtaaaaca tagcgcaatt tctctggatg ttctttttta
     6541 gcttcttctt ccaagtgttc aatcaaccaa ggtgtatcaa cgacaaagat atctgtagac
     6601 atattgaacg tttcagctgt tgacttgcta tcaaagagtt tatgagaaag aacatggtct
     6661 gtttcatcta catccaagat tgcatttact tctgaaatat ctttcttagc tagtttttta
     6721 taaactacag tgataggctc ttttgttgta ctatgtaggt ggaaaacttg gttcaaatca
     6781 atgttaataa gaacatcgca gttgagggca accgtttggt ttgagccaga acgtttcaaa
     6841 taagtaagaa gctgttggta gtattctttt ccaactgtac tactttctac acgggtattg
     6901 taaattccta gatagtaatg gctaagaagg gttgataagc cccactcgcg tcctgaacga
     6961 atatggtcaa atactgagct gatattatcc tgctggaaaa taccaaagac actacgaaca
     7021 cctgcattag caaggcttga aagtgggaag tcaatcaaac gatatttccc accaaatggc
     7081 aaacttgcta ctggacggtg gtccgtcaat gtcgacatat tgtgaaaacc aactgtattt
     7141 cctaaaatgg cagaatattt atcaatcttc atctgttgct acccccacta cttcattata
     7201 tcctacaact tgtacttcat ctgttccatc aatttcgaca ccgtcagaaa taatcgcacc
     7261 ttcaccaata atggcacgtt taatcttagc tccttgacca atgatagctc cactcatgat
     7321 aactgaatca aggacttccg ctccttcgcg aacttgcgcg cctgttgaaa ggatagaatg
     7381 tttaacagtt ccatcaacga aacatccgtc tacaactaat gagtcttcca catgagcatt
     7441 tgccccgagg aagtttggtg gtgaaatcaa gtttcttgag taaatcttcc attgacggtt
     7501 acgactatcc aaggcatttt ctggagaaat atactccatg ttcgcttccc aaagtgactc
     7561 aatagtacca acatctttcc aataaccact aaattcataa gcataaacac tttcacctga
     7621 ctcaaggtaa tttggaatga catttttacc aaagtctgac atgccaacct tgctcttttc
     7681 agcagcgact aacatattac gaaggcgttg ccaatcaaaa atgtagattc ccatagaagc
     7741 ttttgtagat ttaggttgag ctggtttttc ttcaaattca acaatacgat tgttagcatc
     7801 tgtgttcatg ataccaaaac ggcttgcttc tttaagaggg acgtctaaaa ctgctactgt
     7861 caagctggca ttattatcct tatgagactg gagcatatca tcatagtcca ttttgtagat
     7921 gtggtcccca gacaaaatca agacatactc aggattgaca ctgtcgatat agtcgatatt
     7981 ttggtaaata gcgtgactag tcccctcaaa ccaacgattt ccttcacttg cagaataagg
     8041 ttgaagaata gagacacctg aattaatacc gtctagtccc cagcttgaac cattcccaat
     8101 atggttgttg agagcaagtg gttgatactg tgtaacgacc ccaacattat gaatccctga
     8161 gttggcacag tttgataggg caaagtcaat gatacggtag cgcccaccaa attgcacagc
     8221 tggttttgcg atgctttgag tgagtttacc gagacgagtt ccttgcccac cagcaagaat
     8281 caaagctaac atttcatttt tcattttcta ctcctttttg gtttttattt gtgacggttt
     8341 tagtagattt caagcgacgt ttgattttcc atacacttgc tcccatagcc ggtagagtaa
     8401 aggttaaggt ctgctcataa tctttccata gtccttcttg cgtttgaaca gtttgattat
     8461 gttctttcca aacgcctccc cactcttcca actcagtatt ccatacttct tcgtaaattc
     8521 ctgcaacggg tagtccgatt gtaaaatctt tccgctcaac aggtaccata ttaaagatac
     8581 agactaacat ttctcccttt ttacccttac gaataaagga aagaacactc tggtctcgat
     8641 tatccgcatc aatgatttca ataccatcat agctggtatc aatttcccac agacagcgat
     8701 gatctttgta aaactggttt agctgagaag cgaaatactt catcttagca ttcattgggt
     8761 cttctaggtt agaccattcc aactgttctt cagatttcca ttctaggaat tgaccgtatt
     8821 cgctacccat gaagagcaat ttcttaccag ggtgacaaat ttggtacgta tagagattgc
     8881 gcaagcctgc gaattgattg taacgatctc cccacatctt atgcatcata ctcttcttgc
     8941 catgaaccac ttcatcgtgc gagaatggca agagataatt ctccttgaaa acatacataa
     9001 agctgaaagt caccaggtta aagtcatatt tacgataaat cggatcttct tcgtagaaac
     9061 ggaggatatc attcatccag cccatgttcc atttgtagtc aaatcctaga ctaccaatct
     9121 ctttcattcc cgtaatcttg gtcgcagacg aactttcttc tgcaatcatc atcacatctg
     9181 gatattctaa cttaataact tcattcaagc gttgaagaaa ataataacct tcatagttga
     9241 gatttccgcc atctttatta ggtgtccatg gagcattatc atagtccaaa tagagcatgt
     9301 tgctaacagc atccacacga ataccatcca aatgatagac atcaatccaa tgcttaatgc
     9361 aagaaattaa gaaggactgg acttcatttt ttccaaggtc aaaattaagg gcaccccaac
     9421 catggttatg agccttatta tggtcttggt attcaaaagt cggtgtccca tcataatagg
     9481 ctaaggcatc atcgttgatg gtaaagtgac ctggtaccca gtccacaata accccaatat
     9541 tatgggtatg acactcctcg acaaaatctt gaaactcctc tggtctgcca taagcatgct
     9601 ctaaagcgaa gtaacccata agctgatacc cccaactcaa gcccaaagga tgggacatca
     9661 agggcataaa ctcaatatga gtatagttca tttcaacgag ataaggaatg agttcatcct
     9721 tgagctgggc aaaactataa ggactgccat cagaatttct tttccatgat ccagcgtgaa
     9781 cttcataaat attgacagga cgctcttcaa agccccaacg ttttcttcgt gccagccaaa
     9841 gtccatcctt ccatttcttc tcaggaagct ctgttaagat tgcccctgtt cctggacgag
     9901 cctcatacct gacagcaaaa gggtcaatct tcatcagttg atgaccattt tgacgtgtga
     9961 catgatattt gtaaatatgc ccttcttgag ccatattggt aaagacttcc cagaccccaa
    10021 aatcatttct taccattgga atctgatttt caatccagtt ggtaaaatca ccaaccaagt
    10081 gaacagcctg agcattaggt gcccaaacac ggaaggtata gccatgctct ccatttagtt
    10141 cttccctatg tgctcctaga taatgttgga gataaaaatt ttcacccgtc ataaaggttt
    10201 ttaatgcttc tctattatcc atatactccc cttctcctgt aagcgttttc tatgttttta
    10261 ttatactacc tttttagaga agattcaagt aaattactat acttctttaa ttattttgaa
    10321 aatctacaac aagttcactt actcgttcaa ttgtaaatca atattttttc aaaaaattgc
    10381 gaaaacgcct ttctttttct actatagtga aatgaaataa aacatgcgca agtcgattaa
    10441 ggaatttaat ctaatttcta acaatgtctt agaaatcaaa gtgtactatt ttaacttcaa
    10501 tgtacta
//
LOCUS       DAVBNO010000066        10492 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVBNO010000066 DAVBNO010000000
VERSION     DAVBNO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.066"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(26..628)
                     /locus_tag="VQ846_001814"
                     /pseudo
     CDS             complement(26..628)
                     /locus_tag="VQ846_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931989.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(1214..1990)
                     /locus_tag="VQ846_001815"
     CDS             complement(1214..1990)
                     /locus_tag="VQ846_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET3076347.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            2112..2858
                     /locus_tag="VQ846_001816"
     CDS             2112..2858
                     /locus_tag="VQ846_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET3076348.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     RQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            2871..3851
                     /locus_tag="VQ846_001817"
     CDS             2871..3851
                     /locus_tag="VQ846_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET3076349.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            4046..4366
                     /locus_tag="VQ846_001818"
     CDS             4046..4366
                     /locus_tag="VQ846_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET3076350.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            4412..4720
                     /locus_tag="VQ846_001819"
     CDS             4412..4720
                     /locus_tag="VQ846_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET3076351.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            4737..6035
                     /locus_tag="VQ846_001820"
     CDS             4737..6035
                     /locus_tag="VQ846_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET3076352.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            6186..8624
                     /locus_tag="VQ846_001821"
     CDS             6186..8624
                     /locus_tag="VQ846_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET3076353.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            8643..9311
                     /locus_tag="VQ846_001822"
     CDS             8643..9311
                     /locus_tag="VQ846_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET3076354.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            9329..10417
                     /locus_tag="VQ846_001823"
     CDS             9329..10417
                     /locus_tag="VQ846_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET3076355.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
BASE COUNT         3437 a         1487 c         2087 g         3481 t
ORIGIN      
        1 aatttgtcct gttcttattt cattttacta taccatcaat aagtgtgaaa agatctttta
       61 cataagacca agccttatta tctttgtaac caccttgagc ttgtagcata tttgttaatt
      121 gagcaaaggc gctagaagag tttgctgggt cagcagttgc gatttttcct tttagttcag
      181 gtttgagaag gtcgctatat ccttcgatgt tcatgccttt agttaaatca gggtggacga
      241 ttaaaacact accatctagt gtataaggag tagagaagcc agttgtgttt tgatattctt
      301 tgataacatt atcattttct tttgaaatat agttttcaaa gagttctccg tgggtagcat
      361 attgtgtata agaaccacca aagataacat cagctacagg aacttctttt tctgactcta
      421 gttttttgaa aagttctcca gtaccagctt gaatcagttc tattttgata ccatattttt
      481 cttcaaaggc aggaatagtt gctccaatta agccttctga gtttggtgaa taaacgacta
      541 gcgaaccacc gtctccttta tcagatgaac tgtcatcggc agattcatta gaagaacaag
      601 cagcataata catccatttc tttttcatga tggatacctc cgttgtgtta tttaagttta
      661 ttttaaaaca atgtaagcgt ttttaaaacc tacaattcta ttctatagtg tattgaatct
      721 ataacagtac accttgactg ctaaaatatt tctataaatt aatttgactt tcctgataga
      781 gatgttcaca tcttatttca attcactata ttagagtaaa attctctaca aaaaaaagaa
      841 tagcctattt tactattttt ccgagtgatt tcaattcctt tggggaaata tggagatact
      901 ttttaaatcc tgacaaatgg ttgtttcttt ttctaaatcg gtgatactgt atcggagaat
      961 gcgcgtgagg tcacaaaggc tgcgatagag cttctatgga gaatttcttt ttggagagat
     1021 tttttaaagg aatgagacat ccgctacctc cttggaaggt tttttgtgat aagtatagca
     1081 gtttaaaaat tctagagcaa ggtaaaatag aaaaggtttg gctaaaattt tttagctaaa
     1141 ccttttgaaa agttaagtat gtattataga tgcttgttat ctgcaaatat ctagagcatt
     1201 tcaaggaaat aaactagaaa taacaattaa tatggtggtt cagaaatacc ttttgataat
     1261 caataagatc ttcaggatga agtgcgttga ctccatccat ttcatatttc cgatttaata
     1321 aacgatattt gttttcacca aattgatgaa aagggagtag ttgaacttgg tcgatattta
     1381 atgagttaaa tagagtagcg aatttttctg catcctctaa actattgtta aaattaggaa
     1441 taactgggat tcttaaaacg atagttttat tttgagaaaa agcataatga atgtttttaa
     1501 taatcatttg attaaaaacc ccagtcactt ttttatgttt tatagaatta taatgtttta
     1561 ggtctgtgta gataaaatcc acatattgaa ttaaatcgat aaatttttca tgatcaacaa
     1621 aggcagtagt ttcaatggca gtgtgtatgt gatgttcttt agctgatttt aagatggctt
     1681 tagcaaattc aaactgagca aatatttcac ctcctgataa agttaaacct ccaccggatt
     1741 cttcgtaaaa ttctttgtct tttaatacct ctgtaataat ttcttctaca gtcttttctt
     1801 caccgactaa ggtgaatttc tctcgttgag catctttcat tttttcaggt ttcattcttt
     1861 gagattcagg attagaacac catggacagc gcagaggaca tccttttaaa aaaacagttg
     1921 tacgaatacc cgggccgtca tgaattgaaa agtgttgaat attaaaaata attcctttag
     1981 atatttccat aagagttcct cctcttcata agtctatcct atcacaaaaa cgaaagaaaa
     2041 acaattacga acgaaaggtt aatttgttgt tttttgcttt ttattttggt agaataaaac
     2101 ggaggtgtaa aatgaaaaga ttggaacaaa ttattaaatt agtatcagaa tatgaaaaga
     2161 tcgacgttaa tacattatcg gaaaaattaa atgtatcgaa agtaacgatt agaaaagatt
     2221 tagataaatt agagtcaaaa ggtttattac acagagagca tggatatgct gtattaaata
     2281 gtggagatga cttaaatgta cgtttgtcaa ttaattatga aattaagaga aaaattgttc
     2341 aggaagcagt aaaattggtg tcagataatg aaacaataat gatagaatct ggatcgacct
     2401 gtgctttact tgctgaggaa atttgcaagc aaaaaagaaa tgttacgatt gtaacaaatt
     2461 cattttttat agcaaatttt gtgagagctt atgattcatg tcgtattatt gttcttggtg
     2521 gagagtttca gaaggattca caggttactg tgggaccttt attaaaagaa atgatacaga
     2581 cttttcatgt gcgtcaagct tttgttggga cagatggcta cgataaagag atgggcttta
     2641 caggaaaaga tttaatgcgc agtgaggtag ttcaatatat ttcagcagcg tcggataaag
     2701 tcattgtact aactgactca agtaaatttg ataaaagggg tacagtaaga agatttgctt
     2761 taagtcaagt ctatgaagta ataacagacg aaaaactttc taaacaaaat atagctacat
     2821 tagaaaatgc tgggataatg gttaaggtag tttcgtaaga ggttaagtgt atgaatcaag
     2881 ataggaataa actgctctct aaaattgctt atctgtatta tattgaaaac ttaaatcagt
     2941 cacaaatagc agcaaaatta ggaatttata gaacctctat tagtagaatg ttaacagaag
     3001 caaggaatgc aggaattgtt aaaattgaaa tagagaattt tgataccaat atgtttaagt
     3061 tggaaaatta tgtaaaagaa aaatatggtc tggaaagttt agaaattatt ccaaatgaat
     3121 ttgatgataa tccaacaatt ttatctgaaa gaatttctca agttgcagca ggtgtcctta
     3181 ggaatctaat tgatgataat atgaaaattg gcttttcttg ggggaaaagt ttaagtaatt
     3241 tagtagattt aattcacagt aaaagtgtcc gaaatgttca cttctatcct ctagcaggtg
     3301 gtcctagtca catacacgct aaataccatg tgaatacact gatttatgaa atgtctagaa
     3361 aatttcatgg agagtgtaca tttatgaatg caacgattgt gcaagaaaat aaattgttag
     3421 cagatggtat tttgcaatca agatattttg aaaatttgaa aaatagttgg aaagatttag
     3481 atatagctgt agtcggaatt ggtgatttta gcaataaagg aaagcatcaa tggttagaca
     3541 tgcttacaga ggatgacttt aaagaattga ccaaagttaa aactgtagga gaaatttgtt
     3601 gtcgattttt tgattcaaaa ggtaaagaag tctatgaaaa cttacaggaa agaacgatag
     3661 caatctcttt ggaagattta aaaaatattc ctcaaagttt agctgttgct tacggtgata
     3721 cgaaagtatc ttcgattctt tctgtcttgc gtgctaattt agtaaatcat ttgattacag
     3781 acaaaaatac aattttaaaa gttttggaag aagatgggga tttgactttt agagagattc
     3841 taggtgagtg aaaatgatag actgattcag tttatcgttt ttctttttag ttgattgcac
     3901 atttgtgctt atataaacaa aaatagttta tctgttgttt ttggattgac aactttatta
     3961 tgtagttgta ttctatagtt acaaaagaaa attttaaaat ttcaaatgaa aaaagctttt
     4021 tacatagtga aatgaggagg aatttatgga aattattgtt ccagatcaaa ttatcatggg
     4081 tttaatttta tatgctggcg atgcgaaaca acatatttat aaagcattag attacataaa
     4141 gaacggtaca tgtgaacgat gtgaagaaga aatccagtta gctgatgccg ccttattaga
     4201 agctcataat ctacaaacaa aattcttggc acaggaagcg tctggtacaa agacagaaat
     4261 tacagctctc tttgttcatt cacaagatca tctcatgact agtatgacgg agattaattt
     4321 aatcaaagaa attattagtt tgagaaaaga acttcataaa aaataatact agagtattat
     4381 cattgttatt aacatagagg aggaaaacat aatggtgaag attggtttgt tttgtgcagc
     4441 aggtttttct actggtatgc ttgtaaataa tatgaaaatt gcagcgcaag ctagtggagt
     4501 tgaggcagaa atagaggcgt tttctcagtc taaattagcg gattatgcgc caaatataga
     4561 tgttgcacta ttgggtccac aagttgctta tacattagat aaatcaaaag aaatttgtga
     4621 taagtgtgat gttccgatag ctgttattcc gatgatggac tatggtatgt tagatgggaa
     4681 aaaagtatta gatttggccc tatctttgat tagtgggtaa gaaaaggaga tttattatgt
     4741 caaagatgga tgttcagaaa atcattgcac cgatgatgaa gtttgtgaat atgcgtggca
     4801 ttatagctct aaaagatggg atgttagcaa ttttgccatt gacagtagtt ggtagtttgt
     4861 tcttgattat gggacaattg ccgttcgaag gattaaataa gagcattgct agtgtttttg
     4921 gagctaattg gacagagccg tttatgcaag tatattcagg aacttttgct attatgggtc
     4981 taatttcttg tttttcaatt gcctattctt atgctaagaa tagcggagta gaggctttac
     5041 cagctggagt tctatctgta tctgcattct ttattttgct aagatcatct tatatcccta
     5101 aacaaggtga ggcgattggg gacgctatta gtaaagtttg gtttggaggc caaggaatta
     5161 tcggtgctat cattataggt ttggtagtag gaagtattta taccttcttt ataaagagaa
     5221 aaattgttat taagatgcca gaacaagttc cacaagctat tgccaaacag tttgaagcaa
     5281 tgattccagc atttgtaatt ttcttatctt ctatgattgt atatatttta gcgaagtcat
     5341 tgactaatgg cggaacattc atagaaatga tttattctgc tattcaagtt ccgttgcaag
     5401 gtttaactgg atctttgtat ggtgctattg gaattgcatt ctttatatca tttttgtggt
     5461 ggtttggtgt tcatgggcaa tcggtagtaa atggagtagt gacagctctg cttttatcta
     5521 atcttgacgc taataaagct atgttagcct ctgctaatct atcattagaa aatggtgcac
     5581 atattgttac tcaacaattt ttagattcat ttttaattct atcaggttca gggattacgt
     5641 ttggtcttgt agttgccatg ctttttgcag caaaatcaaa acaataccaa gccttaggaa
     5701 aagttgcagc ttttccagca atatttaacg taaatgagcc agttgtattt ggatttccga
     5761 ttgtcatgaa tccagttatg tttgtacctt tcattcttgt tcctgtactt gcagctgtga
     5821 tagtatatgg agctattgca acaggtttca tgcagccatt ctcaggggta acattgcctt
     5881 ggagtacacc agctattcta tcaggatttt tggtgggtgg atggcaaggt gttgtgactc
     5941 agttattagt attggtgata tctacagtgg tttactttcc attctttaag atacaagatc
     6001 gtttagctta ccaaaatgaa atcaaacaat cttagaggta tttgtgtgtt actgttaaac
     6061 tcacacattt gtgctaaaaa ttagagagtt aaaatttttc tagttaaaag cttgaaaatt
     6121 tctataaaaa tcggtattat attttcgaaa gaaataaaaa tattttcgaa agaaaggtgc
     6181 ttacgatggt aaatacagaa gtagcaagaa caacaatcaa gacagaatat tttggcagcc
     6241 ttactgaaag gatgaacaaa tatcgagaag atgttttaaa taaaaaacct tatattgatg
     6301 ctgagagagc agttctagca acacgcgcct atgaacgata caaggaacaa cctaatgtcc
     6361 taaaacgtgc atatatgctg aaagaaattt tggaaaatat gtctatctat attgaagaag
     6421 aatctatgat tgcgggaaat caagcttctt ccaataaaga tgctcctatt tttccggaat
     6481 atacgctaga atttgttctc aatgagttgg atctttttga aaagcgtgat ggagatgttt
     6541 tctatattac agaagaaaca aaagaacaac ttagaagtat tgctccgttt tgggaaaata
     6601 ataatttacg tgctagagct ggtgccttat tacctgaaga agtgtcagtt tatatggaaa
     6661 caggattctt cggtatggaa ggtaagatga attctggaga tgctcactta gcagttaact
     6721 atcagaaact tttgcaattt ggtttaagag gttttgaaga gcgggctcgt aaagcaaaag
     6781 tagctctaga tttaacagat ccagcaagta ttgataaata tcatttttac gactctatat
     6841 ttattgtaat cgatgctatt aaagtatatg caaagcgctt tgttgctctt gctaaaagtt
     6901 tagccgaaaa tgcaaatcct aaacgtaaga aagaattact tgagattgca gatatttgct
     6961 ctagagtccc atatgaaccg gcaactactt ttgcagaagc tattcaatca gtttggttta
     7021 ttcaatgtat tttacaaatt gaatctaatg gccactctct ttcatatggc cgttttgatc
     7081 aatatatgta tccatatatg aaggctgatt tagaaagtgg taaagaaaca gaagatagca
     7141 ttgttgaacg tctgacaaat ctttggatta agacaattac aattaataag gttcgcagtc
     7201 aatcacatac attttcttca gcaggaagtc ctttatatca aaatgttaca attggtggac
     7261 agactcgaga taagaaggat gctgttaacc cattatctta tttggtatta aaatcagttg
     7321 cacaaaccca tctaccgcaa cctaatctaa ctgtacgtta ccatgcaggt ttagatgctc
     7381 gtttcatgaa tgagtgtatt gaagtgatga aacttggttt tggtatgcct gcatttaata
     7441 atgatgagat tattattcct tcttttattg caaaaggagt attggaagat gatgcttatg
     7501 attacagtgc cattggatgt gttgaaacgg cagttccagg gaaatggggc tatcgttgca
     7561 caggtatgag ttatatgaac ttccctaagg ttctacttat cacgatgaat gatggaattg
     7621 atccggcttc gggtaaacgg tttgcaccaa gctttggtca ttttaaggat atgaagaact
     7681 tttctgaatt agaaaatgct tgggataaaa cactaagata tttgacacga atgagtgtta
     7741 ttgttgaaaa ttctattgat ttatcattgg aacgagaagt tcctgatatt ctatgttcag
     7801 cattgactga tgattgtatt ggtcgtggaa aacaccttaa agaaggtgga gcagtatatg
     7861 attatatatc aggattgcaa gttggaattg caaatttgtc ggattcatta gctgcaatta
     7921 aaaaattggt gtttgaggaa gaacgtataa gcccaagtca gctttggcat gcactggaaa
     7981 cagattatgc cggagaagaa ggtaaggtca ttcaagaaat gttgattcat gatgcaccta
     8041 agtatggtaa tgatgatgat tatgctgaca aattggttac tgctgcttat gacatttatg
     8101 ttgatgaaat tgctaaatat cctaatacac gttatggaag agggcctatt ggaggaattc
     8161 gttattcagg aacatcttct atatcagcca acgtagggca gggacgtgga acattagcaa
     8221 ctccagatgg acgcaacgcg ggtacaccgt tagcagaggg ttgttcacca tcacataata
     8281 tggatcaaca cggccctaca tctgttttaa aatctgtttc aaaattacca acagatgaaa
     8341 tcgtaggtgg ggttctctta aatcagaaag taaatcctca aacgttagcc aaagaagaag
     8401 ataaattaaa actaattgct ttgttacgaa cattctttaa tcgtttacat gggtaccata
     8461 ttcaatacaa tgttgtttcc agagagacgt tgattgacgc tcagaaacat cctgaaaaac
     8521 acagagactt aattgttcgt gttgcaggat actctgcatt cttcaatgtt ctttctaagg
     8581 caacccaaga tgacattata ggacgtactg agcatacttt gtaaaataaa gaggttcttt
     8641 ttatggaatt tatgcttgac acattaaatt tagatgagat taaaaagtgg tctgaaattt
     8701 tgccgctagc tggggtaact tcaaatccca ctattgcaaa aagagagggt tctattaatt
     8761 tttttgaacg aatcaaagat gtaagagaat tgattggctc cacaccctct attcatgttc
     8821 aggtgatttc tcaagatttt gaaggcatct taaaggatgc tcatgaaatt cgaagacaag
     8881 caggagatga tatatttatc aaagtacctg ttactccagc tggattacgt gcaataaagg
     8941 tgctaaaaaa agagggctac catatcactg caacagctat ttatacagtt attcagggat
     9001 tattagctat cgaagtagga gcggattacc tagctccata ttataataga atggaaaatc
     9061 tgaacattga ttcaaattct gtcattcgtc aattagctct tgctattgat agacagaact
     9121 ctcctagtaa gattttagct gcatccttta aaaatgtagc acaagtaaat aatgctttag
     9181 ctgcaggtgc gcatgctgtt acagcaggag cagatgtttt tgaatcagct ttcgccatgc
     9241 catctatcca aaaggcggtt gatgattttt ctgacgattg gtttgttact caaaatagtc
     9301 gttccattta gatagagagg aaatacatat gagaattttt gctagtcctt ctagatatat
     9361 tcagggggaa aatgccttgt ttgaaaatgc caaatcaatt ttggatttgg gaaattaccc
     9421 gattctatta tgcgatcagt tggtttatga tattgttgga aaacgatttg aagattatct
     9481 acataggtat ggtttccata ttgttctggc gctatttaat ggtgaagctt ctgacaatga
     9541 aatcaatcga gttgttgcct tggctgagaa agaaaattgt gatagtatta tcggtcttgg
     9601 tgggggaaag acgattgata gcgcaaaagc tattgcagat ttgattgaaa agcctgttat
     9661 tattgctcca acaattgcat cgaccgacgc acctgtatct gctttatctg ttatttatac
     9721 agatgaaggt gcatttgatc attatctatt ttattctaaa aatccagatt tagttttggt
     9781 tgatacaaaa gttatttcac aagcccctaa gcgtttatta gcgtctggta ttgcagatgg
     9841 tttagcaact tgggttgagg cgcgtgcggt tatgcaggca aatggaaaaa ctatgttggg
     9901 acaacagcaa acattggctg gagttgcaat tgcgaagaaa tgtgaagaaa cgctgtttgc
     9961 agatggttta caggctatgg cagcttgtga agctaaagtg gtgacaccag cattagaaaa
    10021 tattgttgaa gctaatactt tattgagtgg tctaggtttt gaaagtggag gattagctgc
    10081 ggcgcatgca attcataatg gttttactgc attgacaggt gacattcatc atttaacaca
    10141 tggtgaaaaa gtagcttatg gaactttagt acaactatta ttggaaaata gacctaaaga
    10201 agaacttgat aagtatattg agttttacaa aaaaattggt atgccaacaa ctctaaaaga
    10261 aatgcatttg gatcaagttg gatatgatga tttaataaaa gttggtaaac aagcaactat
    10321 ggagggtgag acaattcatc agatgccgtt taagatttcg ccctcagatg ttgctcaagc
    10381 tattatcgct gtagatgcct atgtaaattc aaaataaaca ataaggacta ctgttttcca
    10441 aatggtagtc ttttattgat ccctgtattg aattctatag cagattgaaa ta
//
LOCUS       DAVBNO010000067        10323 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVBNO010000067 DAVBNO010000000
VERSION     DAVBNO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10323
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.067"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            89..1264
                     /gene="mutY"
                     /locus_tag="VQ846_001824"
     CDS             89..1264
                     /gene="mutY"
                     /locus_tag="VQ846_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET3076356.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1320..2024
                     /locus_tag="VQ846_001825"
     CDS             1320..2024
                     /locus_tag="VQ846_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3076357.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2017..3366
                     /gene="vicK"
                     /locus_tag="VQ846_001826"
     CDS             2017..3366
                     /gene="vicK"
                     /locus_tag="VQ846_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET3076358.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3368..4177
                     /locus_tag="VQ846_001827"
     CDS             3368..4177
                     /locus_tag="VQ846_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET3076359.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKNKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4268..4510
                     /locus_tag="VQ846_001828"
     CDS             4268..4510
                     /locus_tag="VQ846_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET3076360.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVANLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4512..4766
                     /locus_tag="VQ846_001829"
     CDS             4512..4766
                     /locus_tag="VQ846_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET3076361.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEGPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4813..5556
                     /locus_tag="VQ846_001830"
     CDS             4813..5556
                     /locus_tag="VQ846_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET3076362.1"
                     /translation="MKKKCFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLVKLT
                     KLLKLTRLLRIIGLIGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDVVPTSIFGKWLAVLLMLVGIGTIGMLTSTLTNFFVKDNPDE
                     QIKLDKLKDELSSQRILIEKQSEKIEELH"
     gene            complement(5846..6832)
                     /locus_tag="VQ846_001831"
     CDS             complement(5846..6832)
                     /locus_tag="VQ846_001831"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET3076363.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7026..9494
                     /gene="gyrA"
                     /locus_tag="VQ846_001832"
     CDS             7026..9494
                     /gene="gyrA"
                     /locus_tag="VQ846_001832"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET3076364.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIALRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEE"
     gene            9494..10237
                     /gene="srtA"
                     /locus_tag="VQ846_001833"
     CDS             9494..10237
                     /gene="srtA"
                     /locus_tag="VQ846_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET3076365.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         3210 a         1752 c         2291 g         3070 t
ORIGIN      
        1 agtagataga aaagttatat actatctatt gaaaatatta taaatcagat acaaagacag
       61 gtgaaacctt ctgtgttata ctagagatat gttagatttg aaagaatacg gtatcgtcat
      121 gtggccggag gagaaagtca tttctttccg tgagaaactt ctcgcttggt atgatgaaaa
      181 caaaagagat ttgccttgga ggagaagtaa aaatccttat cacatctggg tatctgaaat
      241 catgcttcag cagaccaggg tggatacagt tatcccttac tacgaaagat ttttggactg
      301 gtttccaact gtcgaaagtc tggcaactgc gcctgaggag agtttactga aagcttggga
      361 gggcttgggc tattattctc gagttcgcaa tatgcaggct gcagcccagc agattatgac
      421 tgactttggt ggccaatttc caaataccta tgaaggaatt tccagcttga aagggattgg
      481 accttacaca gcaggagcca tttccagtat tgcttttaac ttgcctgagc cagctgtaga
      541 tggtaatgtc atgcgggtct tggcgcgtct gtttgaagtc aaccacgata ttgggattcc
      601 aagtaatcga aaaatttttc aggcaatgat ggagatcttg attaatccag atcgtccggg
      661 tgattttaat caagccttga tggacttagg gtctgatatt gagtctcctg taaatcccag
      721 acctgaagaa agcccagtca aggactttag tgcggcatat cagaatggaa ccatggaccg
      781 ttatccaatt aaatctccta agaaaaagcc ggttcctatt tatcttaaag ccttagttgt
      841 gaaaaattct caaggacaat ttttacttga aaaaaatgaa agcgaaaagc tcttggcagg
      901 tttttggcat ttccccttta tagaagttga taacttttcg caagaagagc agtttgacct
      961 ctttcatcag gttgcggaag aaagtgtgaa ctctggcccc agtccagaag agagtttcca
     1021 gcaggactat gacctagatg ttgattggct tgatgtttgt tttgatacag tccagcatgt
     1081 ttttagtcat cgaaaatggc atgtacagat tgtagcaggt caggtgagtg acttccatga
     1141 tttttcagat agggaagttc gttggctttc accagaagaa ttcaagaatt acccacttgc
     1201 caaaccccaa caaaaaatct ggcaggctta tgcacaagcc aacttagaca gtagccaaga
     1261 ctagctattg tgtcttctga ctattttttt ggtataatag tagagaaaaa ggtgaacata
     1321 tgaaaaaaat actaattgta gatgatgaga aaccaatctc ggatattatc aagtttaata
     1381 tgaccaagga aggttacgaa gttgtaactg cttttaatgg tcgtgaagcg ctagagcaat
     1441 ttgaagcaga gcaaccagat attattattc tggatttgat gcttccagaa attgatggtt
     1501 tagaagttgc taagaccatt cgtaagacaa gcagtgtgcc cattcttatg ctttcagcca
     1561 aagatagtga atttgataag gttatcggtt tggaacttgg ggcagatgac tatgtaacga
     1621 aacccttctc caatcgtgag ttgcaggcgc gtgttaaagc tcttctgcgt cgttctcaac
     1681 ctatgccagt agatggtcag gaagcagata gtaaacctca acctatccaa attggggatt
     1741 tagaaattgt tccagacgcc tacgtggcta aaaaatatgg cgaagaacta gacttaaccc
     1801 atcgtgaatt tgagcttttg tatcatttag catcgcatac aggtcaagtc atcacgcgcg
     1861 aacacttgct tgagactgtc tggggttatg actattttgg tgatgtccgt acagttgatg
     1921 tgactgtacg acgtctgcgt gagaagattg aagatacgcc cagccgacca gagtatatct
     1981 tgacacgccg tggtgtaggg tattacatga gaaataatgc ttgatttact gaaacaaacc
     2041 atttttacca gagattttat ctttatcctg attttgttag gtttcatcct tgttgtgacc
     2101 ctcttattac tggaaaatag acgtgataat attcagttga agcaaatcaa tcaaaaggtt
     2161 aaagatttga ttgcaggaga ttattccaag gttcttgata tgcaaggtgg gtctgaaatc
     2221 accaatatta ccaataattt gaatgacttg tcggaggtta ttcgtctcac tcaggaaaat
     2281 ctagaacaag agagtaagag gctaaatagt attctgtttt atatgacaga tggggttctt
     2341 gcgaccaacc gtcggggtca gattatcatg attaacgata cagccaagaa gcaactgggg
     2401 ttggttaagg aagatgttct gaatagaagc attttggaat tgctcaagat agaagaaaac
     2461 tatgaattgc gtgatttgat tacccaaagt ccagaattgt tgctagattc ccaagatata
     2521 aatggtgaat atttgaacct tcgagttcgc tttgccttga tacgtcgaga gtctggcttt
     2581 atttcaggtt tggtggctgt tttgcatgat acgacggagc aggagaagga agaacgcgaa
     2641 cgaagactct ttgtttccaa tgttagccat gagttacgga ctcctctgac tagcgtaaaa
     2701 tcctatcttg aagccttgga tgagggggct ttgtgtgaaa ctgtagcacc agactttatc
     2761 aaggtttctc ttgatgagac caaccgtatg atgcgcatgg tgacggatct cctccatctt
     2821 tcacgtattg ataatgctac cagtcaccta gatgtggaac tgattaactt cactgctttt
     2881 attaccttta tcctcaatcg ttttgacaag atgaaaggac aggaaaagga gaaaaaatat
     2941 gagttggtga gagattatcc catcaattct atctggatgg aaattgatac agataagatg
     3001 acgcaggttg tcgacaatat tttaaataat gctattaaat attcgccaga tgggggtaaa
     3061 atcactgtca gaatgaagac aactgaagac cagatgattt tatccatttc tgaccacggt
     3121 ttggggattc ctaagcagga tttaccacgt atctttgacc gtttctatcg tgtggatcgt
     3181 gctagaagtc gtgcacaagg tggtacaggt ctaggactgt ctatcgctaa agaaattatc
     3241 aaacaacata agggctttat ttgggccaag agtgaatacg gcaagggttc aacctttacc
     3301 attgtgctcc cttatgataa ggatgcagtg aaagaagaag tatgggagga tgaagtagaa
     3361 gactagaatg agtgaaatag gctttaaata cagtatttta gcgtcgggtt ccagtggaaa
     3421 ttctttttat ctggaaacct caaaaaataa gcttttagta gatgcaggct tgtctggcaa
     3481 gaaaattacc agtctgctag ctgaaattaa ccgtaagcca gaagacctgg atgccatctt
     3541 gattacccat gagcattcag atcatatcca tggagtaggc gttttggctc gcaagtatgg
     3601 tatggatctt tatgccaatg aaaagacctg gcaagctatg gaaaatagta aatatcttgg
     3661 caaggtggat tcttcgcaaa agcatatttt tgaaatgggt aaaaccaaaa cctttggaga
     3721 tatcgacatc gagagttttg gtgtaagcca tgatgcagtc gcaccgcagt tctatcgctt
     3781 tatgaaggat gataagagtt ttgtcctttt gacagataca ggttatgtca gtgaccgtat
     3841 ggcgggcatt gtcgaaaatg cggatggcta tcttatcgag gccaaccatg atgtagagat
     3901 tttgcgatca ggttcttacg cttggcgact caaacaacga atcctatctg accttggtca
     3961 cctttctaac gaggacggtg ctgaagctat gattcggacg ctaggaaatc gtactaagaa
     4021 gatttacctt gggcatttat ctaaggaaaa taatatcaag gaactggctc atatgaccat
     4081 ggtcaatcag ctggctcaag ctgatctggg agtcggagta gactttaagg tttatgatac
     4141 ctcaccagat accgcaacac cattgacaga gatataaaaa gaacgctgag aaggtgttct
     4201 ttttatattg actgaacacc taaaaagtaa tacaatggtg ttaccattaa aaaagggagc
     4261 acaaaagatg actactatta cattaaaagt ttctgaagct gataaaacat ttatgaaagc
     4321 aatggctaag tttgaaggag tttccctttc ggaacttatt cgcaccaaaa ctcttgaagc
     4381 cctagaagat gaatacgatg ctcgtgtggc aaatttagcc tatcaagagt atttagaaga
     4441 cttggaaaaa ggagttgaac ccattacttg ggaagaaatg atgcatgatt taggcttgaa
     4501 ggatgaataa tttgtataaa ttagttccaa caagacgttt tatcaagcaa ttgaaaaaat
     4561 tggaccgtta tacgcagaag ctaattacaa actatttaca aaccaatgtt ttggaaggcc
     4621 caagacgaca cggaaaggct ttggttggta atcgcgttgg tcaatggcgc tatagaattg
     4681 gtaattatcg agttatcgta caaattgtag atgatgaatt agtcgttgct actctagaag
     4741 ttggtcatcg gagagatatt tattgaatta aagctgagta ataacaatat agaaagtaag
     4801 gagagtaaaa atttgaagaa aaaatgcttt tttgcagatt attatgatac aacaattatt
     4861 ttacttgctt taatctctgt tattctggta ttacttggat ttgctgagat gattgatttg
     4921 gacaatccgc cttatagtat tattgattta gtaatctggg gtgtttttgt aattgattat
     4981 agttggcgct tttttattac taaaagaaaa tggcgcttta ttcttgaaaa tatttttgat
     5041 ttattggcta ttctcccttt aaatgctatt ttcacagttt ttcgattagg gcgtatcttt
     5101 cgtttagtta aactaacaaa attactgaaa ctgactcgtt tacttagaat aattggactt
     5161 ataggtaaat tagaaagaaa aattagtaga ttcttacgga caaatggcct gatttatatc
     5221 ttatatgtta atatctttat tgttctagta gggagttcga ttttatccgt tgttgaagaa
     5281 aaatccttct cagatagtct ctggtgggct cttgtgacgg taactactgt tggttatggt
     5341 gatgttgttc ctacttcaat ttttggtaaa tggttagctg tcttattaat gcttgtcggt
     5401 attgggacaa taggaatgtt aacgagtacc ttgacgaact tttttgtaaa ggataatcca
     5461 gatgaacaga taaaacttga taaactcaaa gacgaattat cgagccaaag aatattaata
     5521 gagaaacaat ctgaaaaaat cgaagaacta cattgaatga taaaagactt agttgaaaaa
     5581 atatcataaa ggtgagacaa aacaaaactt gattcgagga gaaatcaagt aagtcttgac
     5641 acaatagaac gcatattatc aggtataaaa acttgatact atgcgtttta tagagaaata
     5701 aaataaaatc tgaacaaatc aattaggaat ttaaagcatt ttataacaac gttttagagt
     5761 gatagggtgc tatttcaact tcaacctact ataatacaga aaaaaaacaa ctccctgatg
     5821 attcaaggag ttgtctatag ttaaattagt ttttagaagc ttcttggaat tctggatttt
     5881 tccatgcttc gtcaataata gcttgcaatt ctttagcaga tgcttgcatt ttttgagttt
     5941 ctgcatcgtt caatggaatg tttactggac gaacgatacc atgtgcacca acaacagctg
     6001 gttgaccgat aaagacattc tcaactccgt attgaccttc ttggaatact gaaagtggaa
     6061 gtactgcgtt ttcatcgtca aggattgctt tagtgatacg agcaagggct actgcgatac
     6121 cgtagtatgt tgcacctttt ttgttgatga ttgtgtaggc tgcatcacga acaccttcga
     6181 acaattcaat caattcagct tcttgaacat tttgagtgtc tttaaggaat tcttcaaggt
     6241 ttacaccagc gatgttagcg tgtgaccaaa cagcgaactc agagtcaccg tgttcaccca
     6301 tgatgtaggc gtgcactgaa cgagcatcca catccaattt ttcagcaagt gcttgacgga
     6361 aacgagctga gtcaagtgaa gtacctgaac cgataacgcg ttctttaggg aaaccagaga
     6421 atttccaagt tgagtaagtc aaaacgtcaa ctgggttagc agcaacaagg aagatacctt
     6481 tgaaaccaga ttcaacaact tgagttacga ttgatttgtt gatagcaagg tttttaccta
     6541 caaggtcaag acgagtttca cctggttttt gaggtgcacc tgcagtgatc acaacaaggt
     6601 cagcgtctgc acagtcagag tattgagctg catagatttt tttaggtgaa gtgaaggcaa
     6661 gggcgtgact aaggtcaagc gcatcaccaa cagctttttc atgcaattgt ggaatttcga
     6721 taattccaag ctcttgtgca attccttggt taacaagtgc aaaagcgtaa gatgaaccta
     6781 cagcaccatc accgacaagg ataacttttt tgtgttgttt agttgaagtc attgttttaa
     6841 acatctcctt aattttatta ggggattttc cctagacaac ttcattctat cacttttaaa
     6901 aaactttgtc acgaatatgc cttatagttc tcgatgtaaa cgttttagtg gtttagaggc
     6961 tgaaatagat gggaatttat ggtataatgt tgttacttac taattgtgaa atgaggcatt
     7021 tattaatgca ggataaaaat ttagtgaatg tcaatctgac aaaggagatg aaggcaagtt
     7081 ttatcgacta cgccatgagt gttatcgtag cgcgagctct tcctgatgtt cgagatggct
     7141 taaaacctgt tcaccgtcgc attctctacg gaatgaatga attgggtgtg accccagaca
     7201 aaccccataa aaaatctgct cgtattacag gggatgtcat gggtaaatat cacccacacg
     7261 gggattcctc tatttatgaa gccatggtcc gtatggctca atggtggagc taccgttaca
     7321 tgcttgtaga tggtcacggg aattttggtt ccatggatgg agatagtgct gccgctcaac
     7381 gttataccga ggcacgtatg agcaagattg ctctggaaat gcttcgtgat atcaacaaaa
     7441 atacagttga tttcgttgat aactatgatg ccaatgaacg ggaacccttg gtcttgccag
     7501 cgcgttttcc aaaccttttg gttaatggag caactggtat cgcggttggg atggcaacca
     7561 atattccacc tcataatctg ggtgaaacca ttgatgcagt gaagttggtc atggataatc
     7621 ctgaagtgac taccaaggac ttgatggaag tcttgcctgg accagatttt ccaactggtg
     7681 ctcttgtcat ggggaaatca ggtatccata aggcttatga aacaggtaaa ggttcgattg
     7741 ccctacgttc tcgtacagag attgaaacga ctaagactgg tcgtgagcgc atcgttgtaa
     7801 cagaatttcc ttacatggtc aataaaacca aggtgcatga gcatattgtt cgcttggttc
     7861 aggaaaaacg cattgagggt atcacagcag tacgtgatga gtcaaaccgt gaaggtgttc
     7921 gatttgttat tgaagtcaag cgcgacgcct cagccaatgt tattctcaat aacctcttca
     7981 aaatgaccca aatgcaaacc aattttggtt tcaatatgct cgctatccaa aatggtatac
     8041 cgaaaatttt gtctcttcgt cagattttgg atgcttatat cgagcaccaa aaagaagtgg
     8101 ttgttcgtcg tacacgtttt gataaggaaa aagcggaagc gcgcgctcat atcttagaag
     8161 gtctcttgat tgcgctagac catatcgacg aagtgattcg tatcatccgt gctagtgaaa
     8221 cggatgcgga agctcaagct gagttgatga gcaagtttaa gctttctgaa cgtcaaagtc
     8281 aagctatcct tgatatgcgt cttcgtcgtt tgacaggttt ggaacgcgat aagattcaat
     8341 ctgagtatga tgacctcttg gctctgattg cggatttagc agatattctt gctaagcctg
     8401 aacgtgtttc tcaaattatc aaagacgaat tggatgaagt taaacgtaaa ttttctgata
     8461 agcgccgtac agagttgatg attggacagg tcttgagtct cgaggatgag gacttgattg
     8521 aagaatcgga tgtcttgatt accctttcta acagaggcta cattaagcgt ttggatcagg
     8581 acgagttcac tgctcaaaaa cgtgggggtc gtggtgtcca aggaacggga gtgaaagatg
     8641 atgactttgt tcgtgagtta gtgtcaacta gcacccatga tcatctgctc ttcttcacaa
     8701 acaagggacg tgtctatcgt cttaaaggtt atgaaattcc tgagtatggt cggactgcca
     8761 aagggctacc agtagtcaat ctcttgaaat tggatgaaga cgaaagtatt cagacggtta
     8821 tcaatgttga gtctgatcgc agtgatgatg cttatctctt ctttacaacc cgtcacggta
     8881 ttgtgaagag aaccagtgtt aaggagtttg ccaatattcg tcaaaatggt ctcaaagcgc
     8941 tgaatttaaa ggatgaagat gagttaatca atgtcttgtt ggcagaagga gatatggata
     9001 ttatcattgg taccaagttt ggttatgcag ttcgctttaa tcaatcagcc gttcgtggta
     9061 tgagtcgtat cgccactggt gtaaaaggtg ttaaccttcg tgaaggagac acagttgttg
     9121 gtgccagctt gattactgat caagatgagg ttcttattat cacagaaaaa ggatatggta
     9181 agcgtacagt cgctactgaa tacccaacaa aaggtcgtgg tggtaaggga atgcagacag
     9241 ctaaaattac cgaaaaaaat ggcttgctgg ccggtcttat gactgttcaa ggggatgagg
     9301 atttgatgat tatcactgat acaggtgtca tgattcgaac caatcttgcc aatatttcac
     9361 aaacaggacg tgcaactatg ggagttaaag taatgcgcct ggatcaagat gctcagatag
     9421 tgactttcac aacggttgcg gtggcagaaa aagaagaagt tgggacagaa aacgaaacag
     9481 aaggtgaaga ataatgtctc aaaaaaataa taaaaagaaa aacaagcgaa aaaatctgct
     9541 gacaaatatc ctagcaggat ttctgatatt actgtcactg gctttgattt ttaatactca
     9601 aattcgaaat attttcatag tctggaatac caataagtat caagttagcc aggtatcaaa
     9661 agaaaaatta gaagaaaatc aggatacaga aggcaatttt gactttgatt ctgtcaaagc
     9721 tatctcttcg gaagctgttc taacttctca atgggatgct caaaaattac cagttattgg
     9781 gggaattgca attcctgaat tggaaatgaa tttgccgatt tttaaaggac ttgataatgt
     9841 taatctcttc tacggagctg gtacaatgaa acgcgagcaa gtaatgggag aaggaaatta
     9901 tagtctagct agtcaccata tctttggtgt tgataatgct aataaaatgt tattttctcc
     9961 tttagataat gctaaaaatg gcatgaagat ttatctaacc gataaaaata aagtttatac
    10021 ttatgaaata cgtgaagtca aacgtgtgac accggatcgt gttgatgaag ttgatgatag
    10081 agatggggtc aatgaaatca cattagtaac ctgtgaagac cttgctgcta cagaacgtat
    10141 tattgtcaaa ggtgatttga aagaaacaaa agattattca caaacatctg atgaaattct
    10201 aacagctttc aatcaaccat ataaacaatt ttattaatac aaatcagtga aatcatggat
    10261 ttcactgatt ttttaagatt ttcataaaaa tatagtaaaa tgaaataaga ataggacgaa
    10321 tcg
//
LOCUS       DAVBNO010000068         9717 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVBNO010000068 DAVBNO010000000
VERSION     DAVBNO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.068"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            198..293
                     /locus_tag="VQ846_001834"
                     /pseudo
     CDS             198..293
                     /locus_tag="VQ846_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(546..1166)
                     /locus_tag="VQ846_001835"
     CDS             complement(546..1166)
                     /locus_tag="VQ846_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET3076366.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1166..1648)
                     /locus_tag="VQ846_001836"
     CDS             complement(1166..1648)
                     /locus_tag="VQ846_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET3076367.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1648..2490)
                     /locus_tag="VQ846_001837"
     CDS             complement(1648..2490)
                     /locus_tag="VQ846_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET3076368.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2585..3385)
                     /locus_tag="VQ846_001838"
     CDS             complement(2585..3385)
                     /locus_tag="VQ846_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET3076369.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDNQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3529..4908
                     /gene="glmU"
                     /locus_tag="VQ846_001839"
     CDS             3529..4908
                     /gene="glmU"
                     /locus_tag="VQ846_001839"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET3076370.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILESLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNKRLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4918..5463
                     /locus_tag="VQ846_001840"
     CDS             4918..5463
                     /locus_tag="VQ846_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET3076371.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5475..5789
                     /locus_tag="VQ846_001841"
     CDS             5475..5789
                     /locus_tag="VQ846_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET3076372.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5806..6498
                     /locus_tag="VQ846_001842"
     CDS             5806..6498
                     /locus_tag="VQ846_001842"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET3076373.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIVSHEVV
                     LVESGIGKVMSAMSVAILAVHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6575..7066
                     /gene="rocS"
                     /locus_tag="VQ846_001843"
     CDS             6575..7066
                     /gene="rocS"
                     /locus_tag="VQ846_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET3076374.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7140..7730
                     /locus_tag="VQ846_001844"
     CDS             7140..7730
                     /locus_tag="VQ846_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET3076375.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            7739..9502
                     /locus_tag="VQ846_001845"
     CDS             7739..9502
                     /locus_tag="VQ846_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET3076376.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3008 a         1687 c         2068 g         2954 t
ORIGIN      
        1 taattttaaa gactttttga ccagcctcat ttttttaact tgatactcag tgaaaagcaa
       61 agattaaact aggaagctag ctgtaggctg ctcaaagaac agctttgagg ttgcagataa
      121 aacttgtgag gtaaccaaca tatataatgt gaagctgacg tggtttgaat agattttaga
      181 agggtatgag tctggaagtt ttaatggata atgcaagatt ccatagaatg ggtaagctag
      241 agttcttatg tgaagagttt gggcataaac ttttaccttt tcctccctac tcatattagt
      301 atagaaaagt gaatctaaaa tagtacataa ctgcttctaa aacattctta taaattgatt
      361 taaattctca aatcatatta ttcagttctt atttcatttt gctctacaat cctgttgaga
      421 agacacgtgt tcatatcaaa aaggtattgg caagttgaaa taccttttta caaggttctt
      481 ttgtcttatt tttgtttcaa ctgactatat ctcctatggt tctagttcag aaggctaggc
      541 tataattatg attgataaga agtatcattc caagtattga gagtgaatgt ttcaaaatca
      601 tgggtttcta taatggtcag gctggcattt gctagaccgc catctttacg aagaagtggt
      661 tctttatagc ctaggagagt acgaagactg gcagtaagat tggcgccgtg tccgacaatt
      721 agaatacgct cagctggact atcttttaat gatttgataa attggatggt ccgctgagtt
      781 gtactataga gggattcggc tccgaacatt cgagtgtcaa attgagcaag atttgaacga
      841 aaagcctgga tttgttgcgg gtaaatagct tccagggttg caattttcaa accttctaac
      901 ttcccaagtt gccattcacg gagattagga acgatttcta aagaacaggg ggtatggagt
      961 tgactttgga taatctcagc agatttgacc gctcgaggta aatcacttga ataaatctga
     1021 tcaaaaggaa tttccttgag atactgacca agtcgtttta gggtttcaat ggattcagga
     1081 agaagaggag aatcaccgct agcaccttga aaacgacctt cttggttcca gagggtacga
     1141 ccgtggcgga caaagtagag tttcattact tgatgtcctc caaaatatct acaaaatctg
     1201 cctttacaaa gctagccaag tcttgtggcg cgacgataat gctgtgtccg acttcgcctg
     1261 cagagacaat cattcgatcc aaatctagag caattttatc gataaaaatg ggataattgt
     1321 gtttctgacg aattccgaca ggattattgg ctccatgaat gtaaccagtt gttttttcta
     1381 agtccttttg tggaatcatg ctcacttttt tattgccaga aattttagct agtttctttt
     1441 cagacaagtg ctgagtgata gggacaattc cgataatcgg tccggtcttg tctcccaaaa
     1501 gcgccaaggt tttgaaaatc tgatctcgtt cataaccttg aggaagctct ccttctaggg
     1561 cattgatttg aatcccctga tgagggatag ctgctttaga taggatttgt tccaccaatg
     1621 tttttttgat tttaactttt tttgccatta tttatattta tcctccaatt gactcatcca
     1681 aataccaagc cagattccca gcgcaaagaa gaaggcgatg atgacataac cgacaagtga
     1741 aagtcctgtg tattggatac tttcagcgtt tcctgcattt ggaattaaga tcaaaagggt
     1801 acttgatagg acgataccga tgatgaaatg atagacgcgt gagtggtagt tgtttaaggc
     1861 ataatccatc aattttgaaa aaacgatgag agttgcacct gcaccaattc caatcggaaa
     1921 gaaggttccc aagagatcaa aagttttaaa accagtcaac ataggagcat agagtcccaa
     1981 aatcaaaagt aaattcgatg ggctgaggcc aggaaccaag acgccaaggg ccaatagtgc
     2041 gcctgctagg acgaagttaa gaaagctggc gcttaaggtt ccaacgacaa aatttaaggc
     2101 atatagtcct aatccagaaa tgataaaggt tgtccataac caagctaaat caatcttgtc
     2161 tcggtcagat tctcgagttg attctttgag gaggctagga actgtaccga taatagctcc
     2221 cgcaaagctc cataatacaa agacctgata attttcaagc aggtattcaa tcgggtagga
     2281 aaataagccg attcccagaa gcataccgat ggcaactgga ataaagtaca aaacattttc
     2341 tttaaggtct ttaaagggat gggccagaaa gccaatcatt cgttcataga ttcctaagat
     2401 tgctgctaga actcctccgg aaattcccgg taggataaat ccaagagcaa ttacaatccc
     2461 tttaataacg cgtgctaacc atgagagcat atatttcctc caactatttc tatttcaaaa
     2521 aatccttact atattgtatc acaatcagac acaaaaagaa aaagcaaatg ataaacaaat
     2581 gcttttaagg ttttaaaaaa agctttcgaa aggtttcttc tttatttttt aagggagaga
     2641 taacgttgat atctaaatcg tggtcaaagc cggcaatttt tcctttagat gtgtattggt
     2701 gaatatcata atctaaatca gttttaggac tgctctccaa aaatcctgag tctgagccgt
     2761 aggacggaat ccaaacagag gtaaacttgc ctgtatcaat actgtgttct tccatgaagt
     2821 agacaccaac gtagatgccg atgtttttag cacctagtga tgctagtttt gctcgaaagt
     2881 tttcgacacc ttcgttcata ttagacatgg ttttgtcttc cacgtcaagc caatagtaac
     2941 tagggctgta aggagaggca gcattgtaga aaacttcggc agccttttcc atttcttgga
     3001 cactttttcc agctacataa gcgtagacag caactgggac attccgcttt tgaagttcag
     3061 tgatatgact cttataggcc ttgtctattc cattgataaa tgaagcatca ttttcttttg
     3121 tcgtttgagc accactgtga acacgaacaa tagcacctga aatattttgt gagagggcat
     3181 cgtagttgat ttcctcagga cgctgccagc cagagaggtc aataatcggt ttgtctaagt
     3241 gtttcaaagc ctgtgcttca atctgtgcta tattggattt tgttttaaac gattggttgt
     3301 cattaagtgg gcgattgatg attaaaatga acatcataat cccaaaaaaa ctaaataaaa
     3361 taagtggatg aatttgtttt ctcatatctt ataattctac cctaaaaatc aaaaaaaatc
     3421 aaaaaaatgg gttaaggaag agactttaga gcattttttc attcaagagt gcggaatgat
     3481 ttgaaatatg gtataataaa agggaatttc tacagaaaag agaagattat gtcaaatttt
     3541 gccattattt tagcagcggg taaagggact cgcatgaaat ctgatttgcc aaaagttttg
     3601 cacaaggttg cgggtatttc tatgttggaa catgttttcc gtagtgtggg agctatccaa
     3661 cctgaaaaga cagtaacagt tgtaggacac aaggcagaat tggttgagga ggtcttggct
     3721 ggacagacag aatttgtgac tcaatctgaa cagttgggaa ctggtcatgc agttatgatg
     3781 acagaaccta tcttagaaag tttgtcagga cacaccttgg tcattgcagg agatactcct
     3841 ttaatcactg gtgaaagctt gaaaaacttg attgatttcc atatcaatca taaaaatgtg
     3901 gccactatct tgactgctga aacggataat ccttttggct atggacgaat tgttcgtaat
     3961 gacaatgctg aggttcttcg tattgttgag cagaaggatg ctacagattt tgaaaagcaa
     4021 atcaaggaaa tcaacactgg aacatacgtc tttgacaaca agcgtttgtt tgaggctttg
     4081 aaaaatatca ataccaataa cgctcaaggc gaatactata ttacagacgt cattggtatt
     4141 ttccgtgaaa ctggtgaaaa agttggcgct tatactttga aagattttga tgaaagtctt
     4201 ggggtaaatg accgtgtggc gcttgcgaca gctgagtcag ttatgcgtcg tcgcatcaat
     4261 cataaacaca tggtcaacgg tgttagcttt gtcaatccag aagcaactta tatcgatatt
     4321 gatgttgaga ttgcttcgga agttcaaatc gaagccaatg ttaccttgaa agggcaaacg
     4381 aaaattggtg ctgagactgt tttgacaaac ggtacttatg tagtggacag cactatcgga
     4441 gcaggagcgg tcattaccaa ttctatgatt gaggaaagta gtgttgcaga cggtgtgaca
     4501 gtcggtcctt atgctcacat tcgtccaaat tcaagtctgg gtgcccaagt tcatattggt
     4561 aactttgttg aggtgaaagg atcttcaatc ggtgagaata ccaaggctgg tcatttgact
     4621 tatatcggaa actgtgaagt gggaagcaac gttaatttcg gtgctggaac tattacagtc
     4681 aactatgacg gcaaaaacaa atacaagaca gtcattggag acaatgtctt tgttggttca
     4741 aattcaacca ttattgcacc agtagaactt ggtgacaatt ccctcgttgg tgctggttca
     4801 actattacta aagacgtgcc agcagatgct attgctattg gtcgcggtcg tcagatcaat
     4861 aaagacgaat atgcaacacg tcttcctcat catcctaaga accagtagga gcctatcatg
     4921 gagtttgaag aaaaaacgct tagccgaaaa gaaatctatc aaggaccaat atttaaactg
     4981 gtccaagatc aggttgaatt accagaaggc aagggaactg cccaacggga tttgattttc
     5041 cacaatgggg ctgtctgtgt tttagcagta acggatgaac aaaaacttat cttggtcaag
     5101 cagtaccgca aagctatcga ggctgtctct tacgaaattc cagccggaaa attggaagta
     5161 ggagaaaaca cagcccctgt cgcagctgcc cttcgtgaat tagaggaaga aacagcctat
     5221 acagggaaat tagaactctt gtacgatttt tattcagcta ttggcttttg taatgagaag
     5281 ttaaaactat atttagcaag cgatttgaca aaagtggaaa atccgcgtcc gcaggatgag
     5341 gatgaaacct tggaagtcct tgaagtgagc ttagaagaag cgaaagaatt aatccaatca
     5401 ggtcatatct gtgatgccaa gacaattatg gctgttcagt attgggagtt gcagaaaaaa
     5461 tagaggaggt cagtatgggt aaatctttat taacggatga aatgattgaa agagctaata
     5521 gaggcgaaaa aatttcaggt cctcctttgc tggatgataa tgaggaaact aagattttac
     5581 caacctcttc ttcccgtttt ggttatgcca atcctaagga tcatggtttt agccaggaaa
     5641 ccttgaagat tcaggtcgaa ccatctattc ataaaagccg tcgcattgaa aataccaaga
     5701 gaaatgtctt caattctaag ttgaataaaa tcttattcgc ggtcatcttt ctcttgattt
     5761 tgcttgtttt agcaatgaaa cttttgtaat agaaaaggaa ttgaaatgaa aataggaatt
     5821 attgctgcta tgccagaaga actggcttat ctggtccagc atttagacaa tgcccaggag
     5881 caagttgttt tggggaatac ctatcataca ggaaccattg tttctcatga agtcgttctt
     5941 gtagaaagtg gaattggtaa ggtcatgtct gctatgagtg tggcgatttt ggctgttcat
     6001 ttccaggtgg atgcccttat taatacgggt tcagctgggg cagtagcaga aggtatcgct
     6061 gttggggatg tcgtgattgc tgacaaatta gcctatcatg acgtggatgt cacagctttt
     6121 ggctatgctt atggacaaat ggcgcaacaa ccgctttatt tcgaatcaga caaaaccttt
     6181 gttgctcaaa tccaagagag tttatctcaa ttggaccaaa actggcatct tggtttgatt
     6241 gctacaggag atagttttgt tgcaggaaat gacaagatag aagcgattaa gtcccatttc
     6301 ccagaagttt tagccgtgga gatggagggg gcagctattg ctcaagcagc gcatgccctc
     6361 aatctcccag tcttagtcat cagagctatg agtgacaatg ccaaccatga agcaaacatc
     6421 ttttttgatg agtttattat cgaagctgga cgtcgctctg cccaagtctt gttgaccttt
     6481 ttgaaggctt tagattaagc ggaaatttga cagtttttct agcttatgat aagatttaag
     6541 taaagaaaag ctagaaaacg tttcagagga tattatgagt attgaaatga ccgtcagtga
     6601 gattgcagag gtcttaggat tatctcgcca agcaatcaat aaccgtgtca aagaattacc
     6661 agaagaagac acagataaaa atgacaaagg ggtaacagtt gttaccagaa gtggcttgat
     6721 taagctagaa gaaatctata aaaaaacgat ttttgaagat gagcctgtca gtgaagatgt
     6781 caaacaacgt gaactgatgg agattctagt ggatgagaag aatgcagaaa ttcttcgtct
     6841 ttatgaacaa ttaaaagcca aggatcgtca gttatcagaa aaagacgagc agatgcgtat
     6901 caaagaccgt cagattgctg agaaagacaa acaattagat cagcaacaac aattgaccct
     6961 tcaagccatg aaggatcaag aaaatcttaa gctagagctg gaccaagcaa aagaagaagt
     7021 ccaatccact aagaaaggct tttttgctcg tttatttgga ggataatcaa ggagctgttt
     7081 aggttaaatg ctaacctgat ggcttctttt gtttgtgaat agtagaggag aggtacagaa
     7141 tggaacgatt agaagattac attgcttttg acttagaatt taatcagcac gaaggacaaa
     7201 cacacttgat tcaagtatcg gcagtgcgtt ttagagatgg tcgggaaatc gatgcctatg
     7261 attcctatgt tcataccagc gtccctctaa aaagctttat caatggtttg accgggatta
     7321 cagctgagac cttaaaagat gctcctccag ttgagaaagt cttagaagaa ttccaagagt
     7381 ttgtgggttc tttgctgatg ataggctaca atgctgctaa aagtgattta cctattttag
     7441 cagagcatgg tttagactat agccaacagt acaaggtgga tttgtatgat gaagcttttg
     7501 aacgtcggag ttcagattta catggtattg ccaatctcaa attgcaaact gttgcctcat
     7561 ttcttggatt tcaaggtcag tctcataata gtttagagga tgctcggatg acggcgcgtg
     7621 tttacgaatc cttcttagag tcggatcaag ctagggaatt gttagagaca gaaagtagct
     7681 tatcaaacaa tccttttggt gtcttggact tgtctcaatt atttgactag gagtgcctat
     7741 gaaacctgaa aaacctaaaa aattgggtct taaacagatt tacctcaatc ttgataaaat
     7801 cctctttcta tttttcttga tttttatgat agtggagtat ttgtgggtac cattcaattc
     7861 atggttggct agcttcttat tagatcagac aggttatctt tttatttcct ataacaatgt
     7921 cttagccatc cttactcatt ctccattgat tagtctagct tttttagcct tgattgtaat
     7981 taaccttctg gttgcttatt ttcaaatagg actcctcttt attggtgctc gtcatctcct
     8041 ctataatgaa aagcgaaggc taattgagta tatccgcaag gttttccatg aaagttttgg
     8101 atttatgaaa aagctaactg gcgctaaagc cttgtttatc tttttctata tagctatgct
     8161 gtttcctttt atacggaaaa tgctgaagat ttattatctt aataaaatcg tcattccaga
     8221 gtttatccaa gcttatttag aagatagata ttggatgtgg tggctgagca tcctacttct
     8281 atctcttatc tttctctatg tctctgtcag attgatgttt gcccttccga agattgtata
     8341 tgatcagctg actgttcgag aagcagtgat gtttagcttg gagaaaacca agaagagagt
     8401 tactttttat gcttggaatt tattttacat cttgctcaaa gcacatttat tattttatct
     8461 tcctttgatt cctttattat tggctcaaac tttggtagat gatatcactc agagagagtc
     8521 tctgattctt ggtattttga attttgtcgt gattaaaaat ctctattata tggctctgac
     8581 ttattttttg gttaaatttg tttcattttt gacaggaaag gagctggata tgcttcctag
     8641 gagagaaaaa gatcacatcg tgcgatgggg tgtcatgact tgtgccagtc ttttctttgc
     8701 cttagaaggt tatatttatc tggaggctcc catggttcat ctacctcaac ttatttctca
     8761 ccgaggggtt tccaatgcaa atgggattca aaatacagta gagtccttgg aaactacagc
     8821 acaactcaaa ccagacttgg tggagacgga cgtgcaggaa acaaaagatg ggcagtttgt
     8881 catgatgcat gatgctaact tgaaaaatct agcaggtatc aataaaagtc ctcaagactt
     8941 aaacttggag gagcttaaag ggattgatat ttttgaaaat ggctaccaga ctaaaatttc
     9001 aagctttgaa gattatctca gtcgagccaa cgaacttggt caaaaattac taattgaaat
     9061 taaaaccagt aaaaaagata gtccagacat gatgaaccgc tttttagccc gttatgctgc
     9121 aaagctcaag atttatggac atcaaatcca gtctttagac taccatgttg tcgaaaaagt
     9181 aagacagtat gatgccgaac tgccagttta tttcatcatg ccctacaatt ctgtctttcc
     9241 taaaacaaga gcgacaggat atactatgga gtactcaacc ttggatgaat attttgtaag
     9301 caaactatgg acaacggatc agaaacttta tgtctggact gtcaatgatt cagaagctat
     9361 cagcaaatct cttcacttag gagtagatgg agtgattaca gatgatttgg aaaaagttca
     9421 gcgggaaata gaagtagccc aagaagatcc agaatatacg gatttgctct tgaaaaaagc
     9481 attggaattc tttgaatttt agtaataaaa aagaggaacg gttgttcctc ccatgatagg
     9541 taagtattct tgtgaatgct tgcttttttg tggttgcaat ccgtttacga gaattgtttg
     9601 tattgttctt gtttcaattt actttatagt gctttgaagt tggaatgcta cactataact
     9661 tataaaatat agtaaaatga accaaaaata gtacacaatg tggtataatc tttttat
//
LOCUS       DAVBNO010000069         9451 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVBNO010000069 DAVBNO010000000
VERSION     DAVBNO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9451
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.069"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..116
                     /locus_tag="VQ846_001846"
     CDS             <1..116
                     /locus_tag="VQ846_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET3076377.1"
                     /translation="TPEQAKQILEYGVRGIVVGGAITRPKEITERFVAGLK"
     gene            140..1063
                     /locus_tag="VQ846_001847"
     CDS             140..1063
                     /locus_tag="VQ846_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclically-permuted mutarotase family protein"
                     /protein_id="HET3076378.1"
                     /translation="MKIDVINHLDSPGLAGMICRRINNRILVYGGSYFPNNDPLKSSK
                     VQSNKIRVYDGKFNLLFEQDGQISPDKGITIEVENEIYYILGSSIYRITIDESVEEIC
                     IGDFDFQLESGYGCYYNGHLFFGFQESYLFNIETKELFKKSDFPVSGRGQGLSVKYQN
                     ELYYLGGANNEAYLDGYKYIFEKDKWEKLEFELPSSVLGASSIQINESDLLILGGFNE
                     IAYNKAVIDLATPGYREEYFSKGRDFFNWNRSLRILDLETGKVDIIANDERFALCGAG
                     FLKTDNGYYVVSGECSPGRRTSDILLLEEEM"
     gene            1145..2773
                     /locus_tag="VQ846_001848"
     CDS             1145..2773
                     /locus_tag="VQ846_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET3076379.1"
                     /translation="MKKIVKLALTAAAVLIAVVGLVACSSGSKKSDSSESGKEKVFYL
                     SGLLQGTGDRIPYVWQNGDGLVPYLLYRTLLMADSKYEKSTPDLAKDWKKSDDEKTYE
                     FTLKDGLKWSDGEALTASDVEFSVKLALKASVINGIFPSTFTKIEGAKDFKDDKADKI
                     SGLTVDGNKVTFKLTAPVGNFEQMMTQFFILPKHKLESENPLELNNSSFWTAPVTSGM
                     YKVKEISAGNFLEYDKNENYENEKPKFDKVVFNYISDPVLALQDGKSYFYSTNKPQEI
                     SQLDAVSTLTKKPIDILFYRYFIANLAGVEGNGDSLIANPKVREAILYAINRDELAKS
                     VFSGIADVNNTGVPSSLEAHLKSANKFEYNPEKAKQLLKEAKYDNSRNLILAYYYKDQ
                     ASQDFMQAVSYQLNEVGIKNEVVQITSDATTGLFKTRKYDLAYKGLSSFGYETWYGEY
                     SSTNTNFKNITNGETSFDELSKKLSETSDVKERNKILASLQKLEQEKLLKLNLFTFKN
                     FLYLNTEKVLIPDDVQFGNPFYKFDYKFEKWDAK"
     gene            2882..3832
                     /locus_tag="VQ846_001849"
     CDS             2882..3832
                     /locus_tag="VQ846_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3076380.1"
                     /translation="MKKYILKKVLFMIPMLLIISFLVFIALDLTPADPLTYMVSPDMA
                     SSAEQIEKLRQQLGLNDPVLVRYGRWLWQLLHGDFGYSIVNGNPISKIVGQALPATFE
                     LAFVSLIISTIVGIVIGVISAVKQNGIIDNVARFLAVIGTAIPQFFFGILILNFFAIQ
                     LRILPIGGRFTSGDFTFINRVEHLILPVMAMSIALVAALMRYTRNSMLDVFNADYVKT
                     ARAKGVPEWKVYFKHIFRNAMRPILVLLIFRLPMLIGGSVIIESVFSWPGIGSIILSS
                     VTAGDYPVIMITTLMISVVVLFASLLVDIMAAILDPRIRY"
     gene            3849..4700
                     /locus_tag="VQ846_001850"
     CDS             3849..4700
                     /locus_tag="VQ846_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3076381.1"
                     /translation="MKPMPLWKRNLKKFYNNKLAFIGFICFMLILLACILAPLLTTYS
                     PDVVDLGSMNRPPSAKHILGTDKLGRDVFARILYGGRVSIQVGMYGAICGAVIGTVLG
                     GIAGYFGGKIDALLVRLAELFLTFPNMIVILLLSSIFGQGVFNLIFVFSVMGWMTTFR
                     MVRNEFMSLKQETYVDVCRAFGFSDSRIIFNNILRNAISPVIVSLSLNVAGFILSEAG
                     LSFLGVGVPSDIPTWGNIINAAKTADVIKNSWWLWLVPGSIITLFVLSINFIGDGLRD
                     IMDPKQQ"
     gene            4713..6695
                     /locus_tag="VQ846_001851"
     CDS             4713..6695
                     /locus_tag="VQ846_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3076382.1"
                     /translation="MNQTVLDVKNLKTEFITDKKAFPIIKDVNFTLERGKVLGIVGES
                     GCGKSVTVNTVLNLLPKNGRIAEGEITYFDEDKPIELQKLKQYGKEFRKLRGENISMI
                     FQDPMSALNPVYTIGNQITEVLHEHYDISKEEANKRAIEMLEKLGIHNAAERMNDYPH
                     QFSGGQRQRIVIAIAMVCNPDILIADEPTTALDVTIQAQILDLLDDLRKEHGTSIILI
                     THDLGVIAQIADEVAVMYAGEVVEVGTVAQIFDQPKHPYTRSLLRSIPNPENMDKKLH
                     VIQGSVPSISEISEKGCRFANRIPWLEKEHHEEEPQLHDLGDGHFVRCSCYKHFFFED
                     KEEETLAEKHVGNVVLEVKNIKKYYYPKKQLFKPLGSPLKALDDVSLELRKGTTIGIV
                     GESGSGKSTIAKSLMKLHEITDGEINIDLNGKTQNIYGIKRKEDLDFRKKVQMVFQDP
                     YASLNPTKKIYNSFDEPMIVHNIGNKEERFERMKESLKMVNVPVEYLERYPHEFSGGQ
                     RQRLCIARALCMKPEILILDEPVSALDLSVQAQVLNYLVEIQNKEDLTYVFISHDLGV
                     VKYMCDYLYVIHKGRIVEAGSREDIYNNPMHIYTKKLLAAIPEVDYHFKEALATKRKE
                     NEVEFKAQYHEFYDEDGRAYDLKQVSPTHFVALKPE"
     gene            6705..8114
                     /locus_tag="VQ846_001852"
     CDS             6705..8114
                     /locus_tag="VQ846_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076383.1"
                     /translation="MKLNKKIYFFDKGIFNPYRMSNVKLKVNQAVKDKEIFFTEEKPW
                     EIRFDNSYPNVFFDDLAGVYRCYYSTFTDDEESEKYSLEERKERQYLPRAKRIVSLCY
                     AESKDGVNWVKPNLGITEFRGSTENNIIGHFLHGTSVFLDKHDADENRRYKMFTKIDY
                     GNGVHFIAVAFSKDGLHFDEYIKVPNFNPRADTHNHIIYDEALNRYVLITRTWRDSLR
                     LPCVSTSADFINWTPIQEILNVCDYENQIYSMPIFKRGDYILGLASVFHEGDQLNKNY
                     DTVDLQLTYSYRHVGWHYLNTDTPLIPRGKGQYGDGEFDCGCIYSSAPVTIGDRTYFY
                     YMGGNGQHTNFRETSLSRAYIEKDHFAYWDIKRPEYPGVLYTNGFIFLNDQVYLDADI
                     AAGGFVTIELFENNHTPMEITASLEKIEDGRYQVLFSEPLPRTQTRLKISFKNAKIYA
                     IEGNLDIFRIESDNALLRG"
     gene            8119..8607
                     /locus_tag="VQ846_001853"
     CDS             8119..8607
                     /locus_tag="VQ846_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HET3076384.1"
                     /translation="MKYDLYDNCIELLKEREVTIEDMAALVIFSQQKYYPELTLDDAS
                     YAIQRVLKKREVQNVIMTGIELDKLAEAQKLSPEFQKIMEKDNPLYGIDEVIVLSILN
                     LYGSIAFTNYGYLDKLKPLILERLNENHEGVCNVFLDDIVGAIAAAACSKIAHNHASD
                     EI"
     gene            8895..9302
                     /locus_tag="VQ846_001854"
     CDS             8895..9302
                     /locus_tag="VQ846_001854"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET3076385.1"
                     /translation="MTVINKFVLMEQAKKVLKNAYFPYSKFPVGAAILFKDGKVITGA
                     NIENVSFGVTNCAERSAIFYGTSQGYRKGDILAIAVAGETEDYLPPCNICRQVMVEFC
                     EPDTLVFLLNGKGNILELRLEELVPYSFSSLEM"
     gene            9334..>9451
                     /locus_tag="VQ846_001855"
     CDS             9334..>9451
                     /locus_tag="VQ846_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET3076386.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDK"
BASE COUNT         3232 a         1340 c         1781 g         3098 t
ORIGIN      
        1 atacaccaga acaagccaaa caaatccttg aatatggagt gcgaggaatc gttgttggtg
       61 gcgctattac tagaccaaaa gagattacag aacgttttgt agcgggatta aaatagcatg
      121 ccagagtaag aggtgtaata tgaaaattga tgttataaat catttagact caccaggatt
      181 agctgggatg atttgtagac ggattaataa tagaatttta gtttatggag gtagttattt
      241 cccaaataac gatcctttaa aaagttcaaa agttcaatca aataaaatta gagtttatga
      301 cgggaaattt aatcttctat ttgaacaaga tggacagatt tctccagata aaggaataac
      361 gattgaagtt gaaaatgaaa tctattacat tctaggctcg agcatttatc gtattacgat
      421 tgatgaaagt gtagaagaaa tctgtatcgg ggattttgat ttccaactag agagtggtta
      481 tggttgttat tataatggcc acttattctt tggatttcag gaaagttatc tgttcaatat
      541 tgagactaaa gaacttttta aaaaatctga cttcccagtt tctggtagag gacaaggttt
      601 atcagtcaaa tatcaaaatg aattgtatta tttaggtgga gcgaacaatg aagcttattt
      661 agatggatac aaatatattt ttgaaaaaga taagtgggaa aaacttgagt ttgaattacc
      721 aagttcagtt ttaggagcgt caagtataca aataaatgaa agtgatttat taatccttgg
      781 tggctttaat gaaattgctt ataataaagc agttattgat ttagcaaccc ctggctatag
      841 ggaagaatac ttttctaagg gaagagattt ctttaattgg aatcgtagct tgcgaatact
      901 agatttagag acaggtaagg tcgatattat tgcaaacgat gagcgttttg ctctttgtgg
      961 ggcaggattt ttaaaaactg ataatggata ttacgttgtt agtggggaat gtagcccagg
     1021 aagacgtact tctgacatac ttttgttaga ggaggaaatg taacgaagag tttaaatttt
     1081 taattactgc aatacttact taatcacata aataattact taatacaaat aaaggagtat
     1141 taaaatgaaa aaaattgtta aattagcttt aacagctgca gcagttttaa tagctgtagt
     1201 aggattggtt gcttgtagtt cgggaagcaa aaaatctgat tcttcagagt ctggaaaaga
     1261 aaaagtattc tatttatcag gtttgcttca aggaactgga gatcgtatcc cttacgtttg
     1321 gcaaaatggt gatggtttag ttccatattt gttataccgt acactgttaa tggcagattc
     1381 aaaatatgaa aaatcaactc cagatttagc taaagattgg aaaaaatctg atgatgaaaa
     1441 aacttatgaa tttacactta aagatgggtt gaaatggtct gatggagaag ctttgacagc
     1501 ttccgatgtg gaattcagtg ttaaattagc attgaaagct tcagtaatta atggaatttt
     1561 cccttcaaca ttcacaaaaa ttgaaggagc taaagatttc aaagacgata aagcagataa
     1621 aatttctggt cttacagttg atggaaataa agtaacattt aaacttactg ctccagttgg
     1681 taactttgag caaatgatga ctcaattctt tattcttcca aaacataaat tagagtctga
     1741 aaatcctctt gaattaaata atagctcatt ttggacagct cctgttacaa gcggtatgta
     1801 caaagtaaaa gaaattagtg caggtaattt cttagagtat gacaaaaatg aaaactatga
     1861 aaatgaaaaa ccaaaatttg ataaagtagt attcaactat atttcagatc cagttttagc
     1921 attgcaagat ggtaaatcat acttctattc aacaaacaaa cctcaagaaa ttagtcagtt
     1981 ggatgctgta tctactttaa ctaaaaaacc aattgatata ctattctatc gctactttat
     2041 cgctaactta gcaggtgttg aaggtaatgg agattccttg attgctaatc ctaaagttag
     2101 agaagctatt ctatatgcaa ttaatcgtga tgaattagct aaatctgtat ttagtggaat
     2161 tgcagatgtt aataatactg gtgttccttc aagcttagaa gctcatctga aatcagcaaa
     2221 taaatttgaa tataatccag aaaaagcaaa acaacttctt aaagaagcta aatacgataa
     2281 tagcagaaat ctaattttag cttattacta taaagatcaa gcctctcaag atttcatgca
     2341 agcagtaagt tatcaattga atgaggttgg aatcaagaac gaagttgtcc aaattacttc
     2401 tgatgcaaca acaggattgt tcaaaacacg taaatatgac ctagcttata aaggattatc
     2461 atcatttggt tatgaaactt ggtatggtga atatagttca acaaatacta actttaaaaa
     2521 tattacaaat ggcgaaacaa gttttgatga gttgagcaaa aaattgtctg aaacttctga
     2581 tgtaaaagaa agaaacaaaa ttttagcaag tctacaaaaa ttagaacaag aaaaactatt
     2641 gaagttaaac ttattcacat ttaaaaactt cttgtattta aatactgaaa aagttttaat
     2701 tcctgacgat gttcaatttg gtaatccatt ctataaattt gattacaaat tcgaaaaatg
     2761 ggatgctaaa taaaatcgtt taaaattatc agtgattaag tttgataatc ttttaggagt
     2821 gtagagatgc tgtttattga atcatgtatc tctcactctt gttttttcag gagaaattaa
     2881 aatgaaaaaa tatattctaa aaaaagtatt attcatgatt cctatgttgt tgattattag
     2941 ctttttagta ttcattgcac tagatttaac accagcagat ccattgacat atatggtatc
     3001 tccagatatg gctagtagtg cagaacaaat cgaaaagcta agacagcaat taggtttaaa
     3061 tgatccagta ttagttcgct atggaagatg gctttggcaa ttactccatg gtgattttgg
     3121 atacagtatt gtaaatggta acccaatttc caaaattgta ggacaagcat tgcctgcaac
     3181 ttttgaatta gcttttgtgt cattaattat ttcaacaatt gttggtattg taattggggt
     3241 tatatcagcc gttaaacaaa atggtattat cgataatgtt gctcgttttc tagctgttat
     3301 cggaactgcg attccacaat ttttctttgg tattttaatt ttaaacttct ttgcaattca
     3361 gttaagaatt ttacctattg gtggtagatt taccagtgga gattttacat ttattaatag
     3421 agttgaacac ttaattctac cagtaatggc tatgtcaatt gcattggtag ccgctttaat
     3481 gagatacaca cgtaattcta tgttggatgt ttttaatgca gattatgtta aaacagctcg
     3541 tgcaaaaggt gttcctgaat ggaaagttta ttttaaacac atatttagaa atgctatgcg
     3601 accaatttta gtgttattga ttttccgtct accaatgtta attggtggat cagtaattat
     3661 cgagagtgtc ttttcttggc ctggaattgg ttctatcatc ctatctagtg taacagcagg
     3721 tgactatcct gtaattatga ttaccacatt aatgatttct gtcgtagtgt tatttgctag
     3781 tctacttgta gatattatgg ctgcaatatt agacccacgt attcgttatt aggaaggagg
     3841 tcaatgttat gaaacctatg cctttatgga aaagaaattt gaaaaaattc tataataaca
     3901 aattggcctt tatcggcttt atatgcttca tgctaattct attagcatgt attctagcac
     3961 ctttattgac aacctattct ccagatgttg ttgatttagg atctatgaac cgtcctcctt
     4021 cagcaaaaca cattctggga acagataaac taggtcgtga cgtatttgct cgaatcctat
     4081 atggtggtcg agtttcaatt caagtaggga tgtatggagc gatttgtgga gcggttattg
     4141 gtactgtact tggtggtatt gctggatatt ttggtgggaa aattgatgct cttctagttc
     4201 gtctagcaga attattctta acattcccga atatgattgt cattctcttg ctttcaagta
     4261 tttttggaca aggtgtattc aatcttatct tcgtattctc agtaatgggt tggatgacaa
     4321 cgttccgaat ggttcgtaat gagttcatgt ctttgaaaca agaaacatat gtggatgtat
     4381 gtagagcttt tggtttttct gacagtcgaa ttatctttaa taatatttta agaaatgcta
     4441 tctcaccagt aattgtatcg ttgtctttaa acgttgctgg tttcattctc agtgaggcag
     4501 gtcttagttt cttaggagta ggtgttcctt ctgatattcc tacttgggga aatatcataa
     4561 atgctgccaa aacagccgat gttattaaaa atagttggtg gctgtggtta gttccagggt
     4621 caatcattac tcttttcgtt ctttctatca actttattgg tgatggatta cgagatataa
     4681 tggatccgaa acaacagtag gaggttgaca aaatgaatca aacagtttta gatgtaaaaa
     4741 acttaaaaac tgaattcatc actgataaaa aagcatttcc gattattaaa gatgtaaact
     4801 tcactttgga aagagggaaa gttttaggga ttgttggtga atctggatgc ggtaagagtg
     4861 taacagtgaa tacagtgtta aacttattac ctaaaaatgg acgcattgct gaaggtgaaa
     4921 tcacatattt cgatgaagat aagccgatag agcttcaaaa attaaagcaa tatggtaaag
     4981 aatttcgaaa gttacgtggt gaaaacatat caatgatttt ccaagatcca atgtctgcat
     5041 tgaatccagt atatacaatt ggtaatcaaa ttactgaagt ccttcatgaa cattatgaca
     5101 tttctaaaga ggaggcaaat aaacgtgcta tagaaatgtt agaaaaactt ggaattcata
     5161 atgctgcaga acgcatgaat gactatccac accaatttag tggagggcag cgtcaaagaa
     5221 tagtcatagc gattgcaatg gtatgtaatc cagatattct aattgcagat gaaccaacaa
     5281 ctgcccttga tgtaacaatc caagcacaaa tacttgattt acttgatgat ttacgtaaag
     5341 agcatggaac ttctattatc ttaattactc acgatttagg cgttattgct caaattgcgg
     5401 atgaagttgc agtgatgtat gctggtgaag tcgttgaagt tggaactgta gctcagatct
     5461 ttgatcaacc taaacacccg tatactcgtt ctctattaag atctattcct aatcctgaaa
     5521 atatggataa aaagctacat gttatccagg ggagcgtacc ttcaatttct gaaatatctg
     5581 aaaaaggatg cagattcgca aatcgtattc catggttaga aaaagaacat catgaagaag
     5641 aacctcaatt gcatgattta ggtgacggac attttgtaag gtgttcttgc tataaacatt
     5701 tctttttcga agataaggaa gaagaaacat tagcggaaaa acatgtaggt aatgttgttt
     5761 tagaagtaaa aaatattaaa aagtactact atcctaagaa acaactattc aagccactag
     5821 gttctccgtt gaaagcactt gatgacgtat ctctagagtt aagaaaagga actactattg
     5881 gtatcgtagg agagtctgga agcggtaaga gtacgattgc gaaatcatta atgaaactcc
     5941 atgagattac agatggtgaa atcaatattg atttgaatgg gaaaactcaa aatatttatg
     6001 gaattaaacg taaggaagat ttggatttcc gtaagaaagt tcaaatggta ttccaagatc
     6061 cttatgcatc attgaaccca actaagaaaa tttataattc atttgatgaa ccgatgattg
     6121 tccataatat tggaaacaaa gaggaacgct ttgaacgtat gaaagaatcc ttgaagatgg
     6181 taaacgtgcc agttgagtac ttggagcgtt atcctcatga attttcaggc ggacaacgtc
     6241 aacgtttatg tattgctcgt gccctttgta tgaaaccaga aattttaatt ttagatgaac
     6301 ctgtttcagc attagatcta tctgtacaag cgcaagtttt gaattatctt gttgaaatcc
     6361 aaaataaaga agatttgact tatgtattta tttctcatga cctaggtgtt gtgaaataca
     6421 tgtgtgacta tctatatgtt attcataaag gacgtattgt agaagctggt tctcgagaag
     6481 atatctacaa taacccaatg catatttaca ctaaaaaact attagctgct attccagaag
     6541 ttgattatca cttcaaagag gcattggcaa caaaacgtaa agaaaatgaa gttgaattta
     6601 aagcacagta ccatgaattt tatgatgaag atggccgtgc ttatgatctt aagcaggttt
     6661 caccaacaca ttttgtagcg ttaaaaccag aataggtggt aaatatgaaa ttaaataaaa
     6721 aaatatattt ctttgataaa ggaattttta atccttatcg aatgagtaat gtaaaattaa
     6781 aagttaatca ggcagtaaag gataaagaaa ttttcttcac tgaggaaaaa ccatgggaaa
     6841 ttcgttttga taacagttat ccaaatgttt tctttgatga tttagcaggt gtttatcgtt
     6901 gctactattc tacatttact gatgatgagg agtctgaaaa atactcttta gaggagcgta
     6961 aggaacgtca atatctacct cgtgcgaagc gtattgtttc actttgttat gctgaaagta
     7021 aagatggtgt taactgggtg aaaccaaatc taggtattac agaatttcga ggatcaactg
     7081 aaaataatat tatcggacat tttttacatg gtacatctgt ttttctagat aaacatgacg
     7141 cagatgaaaa tcgtcgttat aaaatgttta caaagattga ttacggtaac ggtgttcatt
     7201 ttattgctgt agcattttca aaagatggtc tacactttga tgaatacatt aaagtaccaa
     7261 acttcaatcc acgtgcagac actcataacc atatcatata tgatgaagca ttaaatcgct
     7321 atgtgctaat tactcgtact tggagagatt cattgcgctt gccgtgtgta tctacgagtg
     7381 ctgatttcat taactggact ccaattcaag aaattttgaa tgtctgtgat tatgaaaatc
     7441 aaatttattc tatgcctatt tttaaacgtg gcgattatat actaggttta gcttcagttt
     7501 tccatgaagg agatcaactg aacaagaatt acgatacagt ggaccttcaa ttgacttata
     7561 gttatcgtca tgttggttgg cattatctaa acactgatac tccacttatt cctcgtggta
     7621 aaggtcaata tggtgatgga gaatttgact gtggatgtat ctattcttct gcaccagtta
     7681 ctatcggtga ccgtacttat ttctactaca tgggaggaaa tggacaacat acaaacttcc
     7741 gtgaaacaag cttgtctcgt gcttatattg agaaagatca ctttgcatat tgggatatta
     7801 aacgtccaga atatccaggt gttttgtata ccaatggatt tattttcctc aatgatcaag
     7861 tttaccttga tgcagatatt gcagctggag gctttgtaac aattgaattg ttcgagaata
     7921 atcacactcc aatggaaatt acagcttccc ttgaaaaaat agaagatggt cgctatcaag
     7981 tgttattcag cgaaccttta cctcgaactc aaactcgact gaaaattagc tttaaaaatg
     8041 ctaaaatcta cgcaatcgaa ggaaatttag atattttcag aattgaatca gacaatgctc
     8101 tattgagggg gtaagattat gaaatatgat ttatatgata attgtattga acttctaaaa
     8161 gaacgcgaag ttacaattga agatatggca gcgttagtaa tattttcaca acaaaaatat
     8221 taccctgaat taactcttga cgacgcttca tatgcaattc aacgtgtact aaaaaaacgt
     8281 gaagttcaga atgtaattat gacaggaatt gagttagata agttagctga agcacaaaaa
     8341 ctatctccag aatttcaaaa gattatggag aaagataatc cattgtatgg gattgatgaa
     8401 gtaatcgtct tatcgatatt gaatttatat ggttctattg cctttacaaa ttatggctat
     8461 ttagataaat taaaaccact gattttagaa agattgaatg agaatcacga aggtgtttgt
     8521 aatgtatttc tagatgatat tgttggtgca atagccgcag cagcatgtag taaaattgca
     8581 cacaatcatg ctagtgatga aatataacta ttgaatttag tatcgtaaat aataaagcgt
     8641 ttggtttcta gattattgtc attcaaattg gataatgata aatctagaaa ccaaatatat
     8701 tttccgcata aaatgaggga ttatatatct cactttttta caattgtata gtggattaaa
     8761 actagaatag tgcatctcta cttctaaaac attgttagaa atcgatttga ctgtcctgat
     8821 tgatttgtac tgttcttatt tcattttact ataatttagt gtgagtaaaa tataataata
     8881 aaggagttaa aacaatgaca gtaattaata aatttgtttt aatggagcaa gcaaaaaaag
     8941 ttttaaaaaa tgcttacttt ccgtattcca aatttcctgt aggtgcagca attttattta
     9001 aagatggtaa agtaattaca ggagcaaata tagaaaatgt atcgtttggt gtaactaact
     9061 gtgcagaacg tagtgctatt ttttatggga cttctcaagg atatagaaaa ggagatattc
     9121 ttgcgattgc tgttgcaggt gaaactgaag actacttacc accatgcaac atttgtcgtc
     9181 aagttatggt agaattttgt gagcctgata ccttagtctt tctattaaat ggtaagggaa
     9241 acatacttga attgcgatta gaggaattag tcccatattc gttctcaagt ctagaaatgt
     9301 aattacgtag tatgaaataa ggagaaaaaa acaatgtcag atttgaaaaa atacgaaggt
     9361 gtcattccag ccttctacgc atgttatgat gatcaaggag aagtaagccc agaacgtacg
     9421 cgtgccttgg ttcaatactt cattgataaa g
//
LOCUS       DAVBNO010000070         8994 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVBNO010000070 DAVBNO010000000
VERSION     DAVBNO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8994
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.070"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(358..1965)
                     /locus_tag="VQ846_001856"
     CDS             complement(358..1965)
                     /locus_tag="VQ846_001856"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET3076387.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2355..2942)
                     /locus_tag="VQ846_001857"
     CDS             complement(2355..2942)
                     /locus_tag="VQ846_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET3076388.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(3070..3519)
                     /locus_tag="VQ846_001858"
     CDS             complement(3070..3519)
                     /locus_tag="VQ846_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3076389.1"
                     /translation="MCFVPYYKVNYCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3524..3913)
                     /locus_tag="VQ846_001859"
     CDS             complement(3524..3913)
                     /locus_tag="VQ846_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HET3076390.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3941..4567)
                     /locus_tag="VQ846_001860"
     CDS             complement(3941..4567)
                     /locus_tag="VQ846_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET3076391.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDPVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4581..5144)
                     /locus_tag="VQ846_001861"
     CDS             complement(4581..5144)
                     /locus_tag="VQ846_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET3076392.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5309..5543)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5597..6100)
                     /locus_tag="VQ846_001862"
     CDS             complement(5597..6100)
                     /locus_tag="VQ846_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET3076393.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6293..7123)
                     /locus_tag="VQ846_001863"
     CDS             complement(6293..7123)
                     /locus_tag="VQ846_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET3076394.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(7108..8790)
                     /locus_tag="VQ846_001864"
     CDS             complement(7108..8790)
                     /locus_tag="VQ846_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3076395.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLQTELVKETSELQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
BASE COUNT         2722 a         2058 c         1637 g         2577 t
ORIGIN      
        1 tggattaaat taaaaaagta taatgtattg aatctataac agtacatctt gactgctaaa
       61 atatttctat aaattaattt gacttttctg atagagatgt tcacatctta tttcaattca
      121 ctatacaatc cagttcctaa aactttgcta gaaattgatt tggctttccg aattaatttg
      181 ttcaaatttt atttcatttc aatatactga taaatccatt cactagcaca gggcaagagg
      241 gagtcgctat tgatttcaac ggtagcaggt acatacagag gactcctacc tcagatatac
      301 tgcaatatca tcgtatgcaa aaaacctctg agatttttct caaaggttct gattttgcta
      361 attgctgttc tcaaccgctg cagtaacaaa ggcagtgtag agttcttctg gtcggtttgg
      421 acggcttgac agttcagggt gatactgaca agctacaaag aatttatttt caggaatttc
      481 cacgatttct accaaacgat tgtctggaga aactcctgaa aagacaaaac ctgctgcctc
      541 aaactgctca cggaaggcat tattaaactc ataacggtga cggtgacggc gttgcaccac
      601 ttcttgattg tgataagcag cagcagcctt agagccacgt ttcaacttag acggataaag
      661 tcccaaacga agggttccac ccatatcctc aatatcaatc tgatcacgca tgatatcaat
      721 gatagggtat tttgtttctg gtgcaagctc tgcagaattg gcaccttcaa gacctaaaac
      781 gtgacgagca aactcgatac atgtcaactg cattcccaag cagactccca acattggaac
      841 atcattttca cgcgcatagc ggatggcttg gattttccct tctgtaccac gttgaccaaa
      901 accacctggt acgatgatcc cgtccgcatc agacaagagt tctgctacat tctctgctgt
      961 cacatcattg gcattgaccc aattgatttt aacttctaca tcattgacat agccagagtg
     1021 tttcaaggct tcgaccactg agatataggc atcttgcaac tccacatact taccaacaag
     1081 ggaaatctta acttgtttct tgaggttcat gaccttgtcc accatggctg accattctgt
     1141 catatccgct gctggtgcgt ctaatttcaa atgatcacaa acaatttggt ccatcccttg
     1201 tgcctgcaag ttcagtggaa tttggtaaag gtgttcaaca tccaacgatt cgataacggc
     1261 ttctggtgcc acatcacaga actgggccag tttattttta attccttgac cagctggctc
     1321 ttctgtacga ataaccaaca tatttggttg gattcccaag ccacgcaatt ctttgacaga
     1381 gtgttgggtt ggtttggttt tcatttcacc agcagccttg aggtaaggaa gcaaggttgt
     1441 atggatatac atgacattat ccgcacccac atctgccttc atctgacgaa gagcctctag
     1501 gaatggcaag gactcgatat ctcctactgt tccaccaacc tctgtgataa tgacatcaga
     1561 gtcggtcgtt agagcggcac gcttgatttt ttctttcaaa gcatctgtga tatgaggaat
     1621 gacttgaaca gttgccccaa ggtattctcc acggcgttct ttacgaagaa cttcactgta
     1681 aattttccca gttgtcacgt tggaatattt gttgagattg atatcgatga aacgttcata
     1741 gtgacccaag tccaaatctg tctcagctcc gtcatctgtc acaaaaactt ccccgtgctg
     1801 gtaaggactc atggttcccg gatcaatatt gatataaggg tcaaactttt gaatggttac
     1861 tttgagacca cgatttttca agagacggcc tagactcgct gccacaatcc ctttcccaat
     1921 ggacgatacc acaccaccag ttacaaaaat atatttcgta gacatagatt cctctttcta
     1981 aaatgctcaa ggtcttgtta actacgaggg gatatagaaa acaagaccct actaataaga
     2041 ataatctggg acgactgcac cagattcaca actaatactc ttcgaaaatc tcttcaaacc
     2101 gcgtcaacgt cgccttgccg tagatatgtg ttcctgactt tgtcagtctt atctacaacc
     2161 tcaaaacagt gttttgagca gcctacggct agtttcctag cttgctcttt gattttcatt
     2221 gagtataaaa tacaagaaga taacttcttg aaaaaacaaa aatagctccc taagaactag
     2281 ggagccccga cctctaaaag aggtgcccaa acaatatgat acctaaaaat aggataattg
     2341 tcaatagcta actcttattc ctcgctggtt tcagcgtcgt catctgaaaa ctcgtcgtcg
     2401 tcttcgttga gatccacatc ttctcccaag tcatctggag cgatttcgtt gatttctgca
     2461 tcataagctt ccacttcatt tttttcatca tctggatttt catcatcgta tgaaagagct
     2521 ggatctgctt cgtatgcatc ttcgtcttct ggatcatctg cattgtagtc aatggcatct
     2581 gaatcaccat ccataaaggc attgacacgt tttttcttag cttttggtgc tacttcatcg
     2641 tcgtcatttt cttcaagagc gatgatttct tcgtcgattt cgtccacacc ataccatgaa
     2701 cgaagacccc atttgttgtc cccaagtgag atgaagctac cgtcaaagtt caactctgtg
     2761 tagaacaaag gcaaagcttc gcggatatcg ctgtttgatg ttccaaggta gttttgaatt
     2821 tcgtttacaa gatcgctaaa atgcatctca tgatcgcgac cacgaagttc caagatagca
     2881 cgcgctacct caatcataga tagttcactt ttttcttgcc cagcaaatac ttctaattcc
     2941 aaagcgtttc tcctcattta tactactatc gccagagcga acagactctg acctcatttt
     3001 atcatttact ctttatttta cgataatttt gcggaatagt caaaggttaa gggggagaaa
     3061 gtggcaggat tagactaatt ccaatataaa actccttcct ttttctgttg ctccattttc
     3121 cacaaatcca agcgacttga aacacctcct agaagcatga ttgtaggtgt agatttcctt
     3181 gactctcaat tctttccatc cttttactcg agccaattca atcaaagcac ttagaatctt
     3241 ttttccaagt cctcgatgtt ggtaagcgga attcccaatc acaatgggga gattatcctg
     3301 agatagtgta atatccccaa ttggaaacca ttctcccttc tccttgactt caatccaaaa
     3361 aagctcacca tgccgatcca aataggaata catggcttcc aaggtcgctt gactgtaagg
     3421 aagcttcacc ccatctacga ggtaaaccaa gttcacatcc tgataccaag caaaagcttc
     3481 ctcacaataa ttgaccttat aataaggaac aaaacacaat gcactacctt ttcaacaaaa
     3541 gagttgctgc ttgattaaaa ccatcacacc agttatacca ttttgcttca tacccatctt
     3601 gagctaggat acgatcttct aaatcaaaaa cagagtaaat ctttctttcc tcgcaagctt
     3661 gcgcatagag atgatatagt tcatcaccac catctctatc ccactcagca gaaatcgtat
     3721 cccgacctgc caataaagcc tgataagccc tgtgatgccc atctgtaatc agcaaacaat
     3781 ctccaaaggc aagaatactg attggatcga cttggattgt ttctgccgac tggtaaagca
     3841 tctgaatatc ttgcaacttc ttttctgata aatatagttg agtcggatga agatctgcta
     3901 tattgacttt catttctttc tcctcaaggg aattcgatac tcacttctgt ttgcctttaa
     3961 atcgccattg gaagcggagc ttgtcataga agggaaactc gataaacagg actcccaagc
     4021 ccacacagag actggcaagg acgtctgatg ggtaatgaac tcccagatag actcttgata
     4081 ccagcacact gactaggtag aggccaagga cgatttgtac gatttttctc cagactggat
     4141 ctttaatccg ctgactaaga ataacaatca aagtacctac catcaaggtt acagccagag
     4201 aatgcccact tgggaaggaa aatcccttct cctccaccag atgtaagata gctggtcgtg
     4261 ggcgctggta gatattttta aaggtcacga ttaaaagacc tgccaaagcc agatttccca
     4321 gcatgaagaa actttctatc ttccatcgct tacgataaaa gacaaaagct gtaatgacaa
     4381 cccaagtgat aatcactggg atatcaatca gacgtgtgag ggatcgaaaa agaatagtca
     4441 aataatctgg taagtctcct cgaatggcag tctgaatcga ttggtcaaaa ttgaccaaca
     4501 tttcagggta aaatttgacc atatagccaa gaataacgaa aagtaaaagg gcaaaactgc
     4561 ccttcattaa aaatgtttgt ttatctttca tagtgtttta aggttggttt caaaaggacg
     4621 tacaacaacc agaatgaaac ggaaaagatt acaccctcaa tcaagttaaa aggtaatacc
     4681 atggtcatta ggtagttgga aagtcccaaa atttttccaa tatcaaagtt agcaaactta
     4741 gcgtacaaag gaacagcata aacatagttg agaaccaaca tggccaaggt taaaccaaca
     4801 gttccagcta gagagcctag taggaaacga agggttgtcc gttccttttt ccaaatcaaa
     4861 gcaaatacga tgacaaaaac tcccaaagct acgatattca tcggcaaacc aatgtaagta
     4921 ttcactcctt ggctgttaag aagcaatttc aatagtgagc gaagcaagag cactcctaga
     4981 gcagcaggca aatccatgac caccagaccc acaaggactg gcaagatact aaattcgatc
     5041 ttgaggaaag atgccgctgg taaaagcgga aagtcaaagt acatcagcac aaatgagatg
     5101 gctgataaaa ttgcaatggt cgaaagtcga cgtgtgtttg tcataacagg ttcctccaat
     5161 tttctataaa atcagaagaa gttggaaagg attcctctat ctattctcac tttttatatc
     5221 ccaaaagttc cctcttactc tattaaagaa aaacaaagca agtggttaca atccggctat
     5281 aaatctatca aaacagacaa agctattctt tcgtcttctc ccatccagac tatactgtcg
     5341 gttgtggaat ctcaccacat cagcttgcgc tcgcggactt ctttaaagag aaggaaatag
     5401 agatttttct tttagactag gcgacaagct gtaggcataa cttctggtta ggcaaagctt
     5461 gtcaacaaag tataaaagga aaagatcatt tcctagttaa agtcaccgcc ggtcgggaat
     5521 cacaccctgc cctgaagact cttttatcat aacaaaaaac gcttgcaagc gcaagcattt
     5581 tgttcatttt cattttttat ttcaatttat ccacttcata ggtatggacg agctcaagac
     5641 ctgcaaagtt ctgctggcga agagcctcat agacaatcat gcagacggta ttagacacat
     5701 tgagactgcg gacatgttca tcattcatgg gaatacggag agccttctca ggatgttctc
     5761 gcataaaatc ttcaggtaag cccttgtctt cacgtccaaa gagaaaataa tggtcctcgt
     5821 cagtcgataa atccacctca gaatagactt tttccgcgaa tttcgaaatc agatagagtt
     5881 ttcccttcat ctgagatatg aaatcttcca aactctcgta aaaataaatc tctagcttat
     5941 cccagtaatc caatccagcc cgcttcatct tgcggtcatc aataggaaag cccataggct
     6001 tgatgatgtg gaggggagaa ttggtcgcag cgcaagtacg cgcgatattg cccgtatttt
     6061 gtggaatttg aggttcaaat aatacaatgt gatttgtcat gacttgcttc ctttcaccat
     6121 tgcaaaaaaa tagccacact gcccggagtc aagctcagca aacagcgtgg ttaaggcatc
     6181 gttaacttac ctcacaacag gtttgaagta aatcagcgaa actactttct tagtataaca
     6241 ctttcagaat cattgtcaat agaaatgact tgattttttc aattttttca agctatttcc
     6301 aagggttgta aaatcgtccc tgattctgca agataagtag taaactaact actaaaaaca
     6361 aggttgccaa gagcaaggta atatagtctc cttttttcaa ggcctgataa ctataccatg
     6421 tgcgtttttt ctctttccca aagcggcgaa gctccatggc agtcgcaatg gtatcaatgc
     6481 gttctagcga gctaaaaatc aagggcgtaa taatgagcag attgcctttg attcgttgca
     6541 taagagaagc tttcttggat aattccatcc cacgcgcctc ctgagacatc ttgatagtaa
     6601 agaattcttc ctgcaaatct ggaatatagc gcaaggtcag gctgacagaa taagcaatct
     6661 tatagggcac accaatttga tttaaactgg aagcaaactg actaggatgg gttgtcatca
     6721 aaaagataat agccagagga atggtgcaaa gatacttaat ggccaaattt agcagataaa
     6781 agagctcctg gctggttaga gtgtagacac cgattccctg ccaaatcaca cttctctctc
     6841 cataaagtcc aaccccatac tcgggagaaa agagatagac catcaaaacg tttaaaacgg
     6901 caaataccgt cgcaaaaacg gctacaaagg aaacatcttt aaagcgaatt tctgataaat
     6961 agaggagaaa gactgaaaag atggcaatca gcaagagcat tctggtatca tagctaatca
     7021 tggccgccaa tgataccaga atgaaaaaga gaagtttccc agctcctgac aagcgatgaa
     7081 tcacagtatc tctatgctgg taaccgatta atttagcttg catccctctc tcctttcttt
     7141 gtaaaatgcc gttaaatcca gtggatccac atctagtttc ttagccaagt taaagatgga
     7201 ggtttctttt agattggctt ttactaacag ctcaggatcg ctcaacagac tggctggaac
     7261 agtatcggca atcaattctc catccaccat gacaaggacc cggtctgaat aatccagcat
     7321 caattgcata tcatgggtaa tcatgacaat ggtatgccct ttttgatgta actcttcgag
     7381 aaattccata atctcagtat agttcttctg atcttgacct gcagtcggtt catctaggag
     7441 aataatttca gctcctaaga ccaaaattga agcaatggtg acacgttttt tctgaccaaa
     7501 tgacagggca gaaataggcc aattacggaa ttcataaagt ccacagattt tcaaggtttc
     7561 atagactctc gtttcaattt ccttctcatc cacacctcgc aaacggagcc ctagagccac
     7621 ctcatcaaaa atcatattgg ttgaaatcat ttgattagga ttttgtagca catatcctac
     7681 tcgttccgcc cgctctgcaa cagaatcgcc ttttatatcc tgtttttccc aaagatagcg
     7741 tccttccgtc tgaataaagc tacttatagc cttggctaga gttgatttcc ctgctccatt
     7801 ttttccgaca atagcaatct tttcaccctt tttaatatct aaatgtaggg attttaaaat
     7861 cggtctatca tcataagaaa aagatacttc ctctagtcta aagagtgact gcaattctga
     7921 ggtttctttt accagttccg tctgtaactg gacttgccct tttgagatag acaagttatc
     7981 cagatctgct aattgttctt ccttgactaa gtccacacct aattgacgga gagtggttag
     8041 ataaaggggt tctcgaattc cattttgagt caataaatca gtcgcaagca actggtcagg
     8101 gctcccatta aaaaggatac gaccatcgtt tatcaagaca atccgatcca cagggcgatg
     8161 cagaacgtcc tccaaacggt gctcgataat cagagtcgtc gtcccctctt ccttatgaat
     8221 ctggtcaatc aattcgataa tatcctgacc tgacttggga tctagattgg cgagtggctc
     8281 atcaaacaag agaatcggac tttcatcaat caagacacca gccagactga ctcgctgctt
     8341 ttgtccacct gacaaatcct gaggacgctg atccagtaaa ggaagaaggt ccagcttttc
     8401 agcccattta taaacacgac ctttcatctc atctagggct gtcacatcat tttccagagc
     8461 aaacgccaaa tcttctgcca cagacaagcc aataaactgc ccatctgtat cctgcaaaac
     8521 tgtgctaacc agatgagact tatcatagat gctcatatca aaggctactt gaccctttat
     8581 caaaaattct ccatatgtct gacccttgta aatattggga ataatcccat tcaaacactg
     8641 acccaaggta gatttacctg acccagatgg tccaacaatt aagactttct ctcccttgta
     8701 aatggtcaag tctatccctt gcaaggtcgg ttcttgttgt gtttcatacc ggaaagagaa
     8761 atccttccac tcaattatag cttctttcat cttactctct tcattcgctt cttagacttc
     8821 tattttatca taaatcaagc ccttcttgca gtctcttctc ttaaaatctt agcgccaaaa
     8881 agattcctat cctagcttac ttgcctaact aatctataaa catcgaaaaa gactagttgc
     8941 ccagccttcc ccatcatttt atactcttcg aaaatctctt caaaccacgt cagc
//
LOCUS       DAVBNO010000071         8327 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVBNO010000071 DAVBNO010000000
VERSION     DAVBNO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.071"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(549..857)
                     /locus_tag="VQ846_001865"
     CDS             complement(549..857)
                     /locus_tag="VQ846_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET3076396.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(854..1162)
                     /locus_tag="VQ846_001866"
     CDS             complement(854..1162)
                     /locus_tag="VQ846_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET3076397.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNTPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(1167..2462)
                     /locus_tag="VQ846_001867"
     CDS             complement(1167..2462)
                     /locus_tag="VQ846_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET3076398.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(2480..3889)
                     /locus_tag="VQ846_001868"
     CDS             complement(2480..3889)
                     /locus_tag="VQ846_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET3076399.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            4047..4775
                     /locus_tag="VQ846_001869"
     CDS             4047..4775
                     /locus_tag="VQ846_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET3076400.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            5234..5428
                     /locus_tag="VQ846_001870"
     CDS             5234..5428
                     /locus_tag="VQ846_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET3076401.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(5793..7103)
                     /locus_tag="VQ846_001871"
     CDS             complement(5793..7103)
                     /locus_tag="VQ846_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET3076402.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            7340..8212
                     /locus_tag="VQ846_001872"
     CDS             7340..8212
                     /locus_tag="VQ846_001872"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET3076403.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         2589 a         1457 c         1583 g         2698 t
ORIGIN      
        1 gatagagttg ttcacatctt atttcaattc actatagttt aatttaagag tagtatttac
       61 taaggcccaa ttaaaatcaa agagcaaacg agaaaacgat gacaaggtgt gttgcttttt
      121 gatttctaaa gagtataatg atagatctct ataaaataag tgcgaaggaa atgagctttt
      181 ataatccttt cgttttaaaa tactatctca gatattctta tatcgacaag aagtttttga
      241 gtcattccct catcatacat attaaataaa tagtggctca ttcaattttt cactagaata
      301 ataagctagt atagtaaact gaaataagat ataaacaaat aaattggagc ttaacatcta
      361 tttccagcaa ttttttagaa attgcagtgg actattctag attcaataca ttataaaaac
      421 tagagtaaaa gaaaaggatt ggatcttgtg taatgcagga tccaatcctt tcaatcattt
      481 tgtccaactt ttggaggttc ctacaatgta gccgtcatta ataaagacag atgggaacga
      541 cagtgttcct atttattttg atagagatcg atgaattctt tagatagcaa ctgaataatc
      601 tctgttgaag ccatttggtc ttctgcatgc ataaatagca aggagaatcc tattttttct
      661 ccagtagctt ctttttgtat gagattagag tgaatcttgt gcgcttctac taaggagtct
      721 tccgcttctt caactttaat tttcgcttct tttaaatttc ccgccttagc tagttggatg
      781 gcttcaataa aggatgattt ggctgctcca ctattggcaa tgagctgaaa acagatatat
      841 tccatttctt ctgtcatctt atttctccta tccatgcaag tgcttgttcc agaacttttg
      901 ctccattcat cattccgtaa tcccgcatat caatggtatc tacaggggta tttcctgcaa
      961 tttctttcac agcaagtaac tcataacgaa tttgtggccc aattagaatg acatctgctt
     1021 catggatatt ctttttagct tctgtcattg attttgcttg gatagaaatt tcaatcccac
     1081 gttcagtcgc actttgttgc atttttttaa caagcatact tgtcgacatt cccgcattac
     1141 atactaataa aatttgtttc ataatcttaa ccttccattt cttgttcaac aactttgtca
     1201 ttaactttga taaatggaat gtatagaaga actccaagtg caaagatgat gaattgaact
     1261 agaactgctc tcacgtcccc tgctgttgct aaccatgcat ttaagaatac tggtgtagtc
     1321 caaggaactt gtataaatgc aggactcatg aatcctgtaa ctgttgctaa gtagctgatt
     1381 aaaataccaa ggactggaac tgtgataaat ggaatagcta atgaaatgtt ataaacgatt
     1441 gggtaaccga ataatactgg ttcattgata ttgaagatac caggtccaaa agataattta
     1501 gccacgtttt tagaaacagc attgcgactc actaagaatg ttgctattaa taaacataat
     1561 gtagatccac taccacccat taaagcgaat gtttgtattt gtgataagtt gatgatgtgt
     1621 ggaatggctt gtccattatt tgctgcagtg atgttttcag taatgttaat taatagtaat
     1681 ggttctagga tggcactgta aataactgct tggtgaatac caaatagcca taacatattt
     1741 cctaaagagt aaataataat gaccccgatt aagcttgtac caatatgacg aattggttct
     1801 tgaataaaga ttgtaatgat tgagattaag ttcattccag ttatattgaa taataatgct
     1861 gaaacaaccc caaataagga gatgacggtc atgactggaa gtaatacgct aaatgatcta
     1921 ctaacagctg gtggaatatt ttcaccaagg ttcatttgta aagctttaac gtttgataat
     1981 tcaatgaata attctgttgc aataatcgct acgataaccc cggcgaacat tgcgcctgta
     2041 cctgtgttgt tgaatgaaag aacacctgaa atgtttaccg catcttttgc tccgtcagga
     2101 actacagaaa ctgtatttgg catcatcaca attaaagaaa ctaatgatag cattgatgct
     2161 gctaacgggt tttcgaaatc tctgttttta gctaagaaat aaccaaccat tacagcaata
     2221 atcatacctg aaatacttaa agtaccgttt gcaattgtta ttccccaata ttggaatttt
     2281 gttaatgtat ccccttggaa aatccactta aataccgtgt tgttcaaaag aacgattaaa
     2341 cctgccaaaa tatataatgg cattactgtt acgaatgcat ctcttagggt ttttaaatga
     2401 atttggttcc ctagtttacc agcaaaggtg gcaaattgat ctataaattt tgagtttttc
     2461 atctgaatat ctcctctctt tactcctcaa tttctacttc aaaaccgtta ttgtcactta
     2521 ctttgcgata ccatcttcct gagctcttga tggttctctt ttgcgtttct aaatcaacgg
     2581 agataaatcc gtaacgattc ttatatgcat tattccaaga ccagcaatca aatgcggtcc
     2641 aagcgtggta tccaaaacag tgacttccct cttcaatggc tttatgtagc catcttaaat
     2701 gttcttcata aaattcaata cgatacacgt catcgataac tccattttca tcgataaacc
     2761 gtgcttcgtt ttcaacaccc attccgtttt cactgataaa ccatggaata ttatcatatt
     2821 cttccttcac aatcatagca atatcataaa tggctttcgg aaaaatttcc caaccacgat
     2881 atggattcat gcgacgctcc ggccattcat actctttgaa gtattggtct ggcatccagg
     2941 gcgtctgata ttcctcagga tttgcttgtg cttgaacacg ttttggatgg tagtagttta
     3001 ctccaagaaa atcaaccgta tttgatttca tcagttgaag ctctttttcg gtatgactcc
     3061 ataacacgcc atctctctct agctgtttta ccaatctttc tgggaaagtt cctttaacag
     3121 ctggattcaa gaagactttg ttaaagaagt cttctgtaaa tcgacttgct tctaagtctt
     3181 ctggagaatt acttcttgga taagcaggtg tcaagtttaa aataatccca atctttccat
     3241 caagtcctaa tgagcgatat agttgaatca cttttgcact agcaaggttt agattataga
     3301 tgacttgtac ggcctctttt ccctttcctt ttagatttgg ataatggaaa gcatataagt
     3361 atcctgcttc tggaatgacc attggctcat tgaaagttgt ccagtaatga accttatctc
     3421 caaagcatgt gaaagcagtc ttggcaaact tcacgaataa ctccactaca tgtttgcttt
     3481 cccaaccacc gtatttttga agaagttcca ctggtaaatc aaaatgatgt aaattcatca
     3541 caagatccat ctggttcttt ttagcttctt caatgatggc attgtagaaa gcaatacctt
     3601 ttggatcagg ctcacctgtc tctaaattct tgatgagtcg actccattgg atggaagtgc
     3661 ggaaagaatt aactccaatt tccttcatca agtggaaatc ttcggtgtaa gtatgaaaaa
     3721 agttactggc tacaagaggt ccgacattat cgaaaaaatc ttctggatgc gttttgaacc
     3781 agtagtccat cacattttca tgtacttttc catattgacc ttctgtctga ggtccgctag
     3841 aagcagctcc ccaccaaaac agattaggaa atactaactt ttttttcatg ctcaacctcc
     3901 tttctattat tctatacata aagtatagca taattttaaa atttgtctag acatttttaa
     3961 aaattgttga tacatatctt gtgtttgcct catttcttga actttgttat actactatta
     4021 gaatattata gagagaacca gattaaatga gtacaaaata tttatttatt tacaatgaga
     4081 ttcgtgaaaa gattctttgt aataaatata ccatgaacga acaattgcct gatgaaatga
     4141 cattagctaa acagtttgcc tgtagtcgaa tgacgatcaa aaaagcttta gacttgttag
     4201 tttctgaggg cttaattttt agaaaacgtg ggcagggaac ctttgttctc tctcgtggca
     4261 gctcaaaaag aaaattaatc gttccagaaa gagatatccg gggactgaca aaaatatctg
     4321 aagatgctca ttctacaatt gactcgagga ttattcactt caaattagaa tttgcaaatg
     4381 aatttttagc agaaaaacta caggtcgctt tgcagagtcc agtttataat atttaccgtc
     4441 tgcgtattat tgacggtaaa ccttatgttc tggaacaaac ttatatgagt accgatgtta
     4501 ttccaggtat tactgaagat attttacaaa aatcgattta caattacatt gaaggaaagt
     4561 taggattgca tattgccagt gctacaaaaa tcttgcgagc ttcttctagt tcagaaaatg
     4621 agcaacatta cttgcagctc cttccaacgg aaccggtatt tgaagtagaa caagtggctt
     4681 atttggataa cggaactccg tttgagtact cgattagtcg tcatcgctat gatttatttg
     4741 aatttaattc ttttgcatta cgacattcct cctaggagaa aatgtgaaaa tgaagccaat
     4801 cttttacaga ctctagttta agaaaaattt aaaacagggc aagaaggtcc catctatgct
     4861 taaatggttt ctcttttcta aataagatgg ctttaaaaga gtgatcgttt tatccatcat
     4921 gttgaaaaat atcttcgtat agcttataga gtaggtactg aaattgttca cctgatctac
     4981 ttcttatagt tatttagttt taaatagtgt ttcaaacatt cttacactga cgagaagttt
     5041 ttgagtcttt tcttgtaaca catatagtat actgaggtta gaatagtaga ctgtgacttc
     5101 taaaaaattg ctagaaatga atttcaatct cccaatttat ttgttcatat cttcttttaa
     5161 tatattaaat aaattctgaa tcataatcat ttaaaaaaat tttatttttt atttttcatt
     5221 acgaataata tagatgaagg ggaaagagta tgaaaacaga actgtttctt ttgctattag
     5281 ttcaaaagga gaaaaaatga aagtagaaaa tatttcgtat agggtggatc atcgtatatt
     5341 gtttgataat atttcttttg atacttcgag ttcaggcgtg acattaatta ctggtaaaaa
     5401 tggtacagga aagtcaactt tactatagta gattgaaact agaatagtac acatctactt
     5461 ctaaaatatt gttagaaatc gatttgacta tcctgatcta tttgtcctgt tcttatttca
     5521 tttcactata tctcaaattg agtatgacga agtgcgctcc catgtcctgg gaacgcactt
     5581 cgtcatattt ttcatattct tgaatccatc gataaagact attgggatga atttttaaag
     5641 ttgaactaat tatttttaca ggatgaggtt tacagcagag agtttgaagg ctttatcaaa
     5701 ggtttttctt ggcataatga cttttcctcg tttccactta attttgtgtc tactttatta
     5761 taccaagtcc acccttaagt tagataatga atctaactta aggaagctag aaggatgaga
     5821 atccaggtgg tcaagagtcc caaacttaag ctgataggga cacccagaat aatttgcttt
     5881 ttgaaggcaa gaccgcgttc ctctatattg gggagtgaga gttgaatgag agaaccagct
     5941 gatgaaaagg gtgagatatt agtagataga gcgccaataa cggtggctgt tgtgagtaag
     6001 tgaatatcaa tctgaggatt ttgagcactg atgatagcaa tgatgggaaa gagggctgga
     6061 gctacaacgg atagggtgga actaaagagt gacatcactc cggctatcac acaaaagaac
     6121 agaggtaacc agaaatgagg aatggttgtt gtcatgaggt gccctatcag tgtgactaaa
     6181 cctgacttga ccgctagaga cattagtaag ctcatgccgc agagcatgat aattgtagcc
     6241 cagggaactt tagccaaaat ggcttcttgc ttccctaatt tgagccttaa ggcgaggcag
     6301 accatgagta ttgagacaaa gccaatatca aatgtttttt gataagtagc tatccaggcg
     6361 atgtttggga aaatgagatg caacaaggga aaaagccaaa ccaaaaccat gctgctgatc
     6421 atgagcaagg tggtttgtct ttgaaccttg ctgaggagtg gtggttggtc aatagtcaag
     6481 gatgagtttg ttcttccctt actatagtgg ctgtaacagg ataataaaag caagacgatg
     6541 agtgggtaga taatgctgac gataaagata tgattgccaa gtgaaaaagc ttgctcttcc
     6601 catcccattt gcttaaacag gccttgaaag acaatgcctg agctactggt tatcaaatta
     6661 gcccctcctg aagctcccca attgacggct tgagctccaa tcaaagggtg tttgtccgct
     6721 ttttgacaga gggtaatcgc tagaggacag caaacggcca tagtagtgaa aaatccagca
     6781 cctaaagcag acaaaagggt tgccatcagg tataaaatca tgtagagggc gttaggatgg
     6841 gtgcgtgtgc ggtagagaat gtgttgagcc aagacatcaa gagtaccgtt agttgttgca
     6901 acgttataaa agagagagac gctaaaaatg gtaaaaaaga gtgaggttgg ccaaaaatga
     6961 agaagttctt tggggcttaa tcccatgaga gtggttgcga tgaggtaaga aaaagcaata
     7021 gccagcaggc caatattgat tttggtgcgg taaccaattc caatggctag agcaatggcg
     7081 ctaatcatta ttaaatgaat cattgaattg tcctttcagt tagaatatgg aaataggttg
     7141 gcaattttgg cgctcaaaag accacctgtt tcctattggt acttgactat tatacacaaa
     7201 aaaaatacaa ttgtcttgac aattgtattg acacttgtta gaattaaaaa tgaggtattc
     7261 aggcttgtga tttcctatgg aagttcatag tgattgcctc tagtgcttac aagtgatatt
     7321 aaaaatagag gatctagtga tgtcaatcat ttcaactgat ttaacccctt ttcaaataga
     7381 tgatacattg aaagcagcct tgcgagaaga tgttcattcc gaagattaca gtaccaatgc
     7441 catttttgat catcatggcc aagccaaggt gtcgcttttt gccaaggaag ctggtgtttt
     7501 agcggggcta accgtttttc aaagggtttt taccctattt gatgccgagg tgaccttcca
     7561 gaatcctcat caatttaagg atggggatcg tttgactagt ggcgatttgg ttttagaaat
     7621 cataggctcg gtgagaagtc tcttaacatg tgaacgcgtt gccttgaatt ttttacaaca
     7681 tttatcaggg attgcttcga tgacagctgc ttatgtagaa gccttaggcg atgattgcat
     7741 taaggtattt gatactcgaa aaactactcc taatttacgt ctttttgaga aatatgccgt
     7801 gagagttggc ggtggctata atcatcgctt taatttatca gatgctatcc tgctaaaaga
     7861 caatcacatt gcggcagtag gtagtgttca aaaggcaatt gctcaagcgc gtgcctatgc
     7921 cccttttgtg aaaatggtcg aggtggaagt ggaaagcctt gctgctgccg aagaagctgc
     7981 ggcggcgggt gctgatatta tcatgttgga taatatgtca ttggaacaga ttgaacaggc
     8041 cattacccta attgcaggac gttctcggat tgaatgttct ggaaatattg atatgaccac
     8101 tattagccgt tttcgtggtt tagcgattga ttacgtctcc agtggtagtt taacccatag
     8161 tgctaagagt cttgattttt ccatgaaggg gttaacctac cttgatgtct aagttgtaaa
     8221 ataaactaac tttttaaagg atgtctttcc tctagaacga gttttatgtc agatagttta
     8281 aacgcctctt caaatatagt aaaatgaacc aaaaatagta cacaatg
//
LOCUS       DAVBNO010000072         7485 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVBNO010000072 DAVBNO010000000
VERSION     DAVBNO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.072"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            158..313
                     /locus_tag="VQ846_001873"
                     /pseudo
     CDS             158..313
                     /locus_tag="VQ846_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            513..3788
                     /gene="rexB"
                     /locus_tag="VQ846_001874"
     CDS             513..3788
                     /gene="rexB"
                     /locus_tag="VQ846_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET3076404.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGKQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3785..7435
                     /gene="addA"
                     /locus_tag="VQ846_001875"
     CDS             3785..7435
                     /gene="addA"
                     /locus_tag="VQ846_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET3076405.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLERFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLSSVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEVKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
BASE COUNT         2277 a         1574 c         1593 g         2041 t
ORIGIN      
        1 cacattgtgt actatttttg gttcatttta ctatagtaga ttgaaataag atacgaacag
       61 cttgattagg gaaatcaaat taatttctag aaatatttta gcatccacgg tgtactatta
      121 tagtttcaat ttactatagt tcaatcattc tatcaagaat attgaatata taaaggcacg
      181 taatattaaa acttcttctg atatcgaatt agagtccgat gttgatggcg ataaatcaga
      241 caatctacca gtcgacatta agatactggg acaacacatt gaagtgtatt ccgtacctaa
      301 gaaacaatca taaaactcaa agccttgtta ccgaggcttt tttattatgt gattttcagt
      361 agccagactt attctatcta atacaagtaa agctaagtat ttaagattta gcagatgatg
      421 aagaaaagaa aggatggcct aaatatttta acagatttac gaggctaatc atggtataat
      481 atagggtgat gaaaaattcc aacgaggctg agatgaaatt actttatact gatattcgga
      541 cttctttgac agaaattcta acaagagagg cagaagagct agttgcagct ggcaagcggg
      601 tcttctacat tgcccccaac tctctttctt ttgaaaagga acgcgccgtg ctggaatgct
      661 tgtcccagca ggcttctttt tcgattaccg tcacgcgctt tgctcaaatg gctcgttatc
      721 tggtcttgaa tgatttacca gctaaaacta ctcttgatga tatcggtctt gggttggcct
      781 tttacaaatg ccttgccgaa ctcgatccca aggacttgcg tgtttatggc gctattaagc
      841 aggatcctca attgatccag cagttaattg agctttacca tgagatgacc aaatcccaga
      901 tgagtttttt ggacttggag aatttaacag atgaggataa gagggcggat ttactcttga
      961 tttttgagaa agtaacagcc tatcttaatc aaggtcagtt agcccaggga agtcagttgt
     1021 cccatttgat tgaggctatt gagaatgaca aggtaagtag tgattttaat caaatcacct
     1081 tggtcattga cggctttact cgtttttctg ctgaggaaga gcgggttgtg gacttacttc
     1141 acggcaaagg tgttgagatt gttatcgggg cttatgctag taagaaagcc tataccagtc
     1201 cttttagcga gggcaatctc taccaagcca gcgtaaaatt tctccatcat ctggcttcta
     1261 aataccaaac gcctgctcag gactgttctc aaactcatga gaagatggat agttttgaca
     1321 aggcctctcg tttgttggag tcttcttatg acttttcaga actcgctttg gatgtcgatg
     1381 agaaagaccg tgaaaattta caaatctggt cttgtttgac gcaaaaggag gagttggagc
     1441 tagtagcccg tagtattcgt cagaaattac atgagaactc agacctgagc tacaagcatt
     1501 ttcgtattct cttgggggat gtagcttctt accagttatc tctcaaaacc atttttgacc
     1561 agtatcagat tcctttttat cttggtagaa gcgaagccat ggctcatcat cccttgactc
     1621 agtttgtcga gtctatttta gctttaaaac gttaccgttt tcgtcaggag gatttgatta
     1681 atcttcttag aactgatttg tatactgacc tcagtcagtc tgatattgat gcttttgagc
     1741 aatatatccg ctatcttggt atcaatggct tgccagcctt tcagcaaatc ttcaccaaat
     1801 cccaccatgg aaaatttaat cttgagcgtt tgaatgtcct ccgcctgaga attttagcac
     1861 ctcttgaaac cctctttgcc agccgaaaac aaaaggctga aaacctccta caaaaatgga
     1921 gtgtctttct aaaagaagga gctgtgacca agcagttaca agatttgaca accactttgg
     1981 aagctgtaga acaggaaaga caagccgaag tttggaaggc tttctgccat gttttagaac
     2041 aatttgcgac tgtttttgct ggttcacagg ttagtctgga agacttccta gccttgctcc
     2101 attctggaat gagtttgtcc caataccgta ccattccagc aacagtggac actgttctgg
     2161 tgcagagtta cgatttgatt gcaccattga ctgctgactt tgtctatgct attggactaa
     2221 ctcaggacaa tttaccaaaa atttctcaaa acaccagtct tctgacagat gaagaaaggc
     2281 aaaacctaaa ccaagcgacc gaagaaggcg ttcaattact gattgccagc agtgaaaatc
     2341 tcaagaaaaa tcgctacact atgctttcct tggtcaattc tgctcgtaag cagttgttct
     2401 tgtcggctcc aagccttttt aacgaaagtg aaagtaagga atctgcctat cttcaagagt
     2461 tgatccattt tggatttagg cggagagaga agaggatgaa tcacaaagga ctgtctaagg
     2521 aggatatggg gtcctatcac agtcttttgt ctagtctggt tgcctatcac cagcagggtg
     2581 agatgagcga tactgagcaa gatttgactt ttgtcaaggt tctgtcgcgt gtcataggta
     2641 aaaaactaga tctgcaaggt ctggaaaatc cagctatccc aaccagtcca agcagcaaga
     2701 ccttagccaa ggacaccttg caagctctct atcctgccaa acaggagttt tacctgtcta
     2761 cgtcgggttt gacagagttt tatcgcaatg aatacagtta tttcctacgc tacgttttag
     2821 gcttgcagga ggaattacgt ttgcgtcctg atgcccgtag tcacgggaat ttcttgcatc
     2881 gtatctttga acgcgcctta cagttgccta atgaagattc ctttgaccaa cgtctagaac
     2941 aagctattca agaaaccagt caagaacgcg aatttgaagc tatttatcaa gaaagtttgg
     3001 aagcccagtt taccaaggaa gttttgcttg atgttgcacg gacgactgga catattctcc
     3061 gacacaatcc agccatcgaa actatcaaag aagaagcaaa ttttggtgga aaagaccaag
     3121 cctttattca attagacaat ggacgcagtg tctttgtacg aggcaaggtg gaccggattg
     3181 accgtttgaa agctaatgga gcgataggag tagtagacta caaatccagt ctgactcagt
     3241 tccagtttcc tcatttcttt aatgggctca attctcagtt accaacctat cttgctgccc
     3301 taaaaagaga agggaagcag aactttttcg gcgccatgta cttggaaatg gctgaacctg
     3361 tccaatctct gatggcggta aaaagtctgg caggagcagt ggtagaagcc agcaaatcta
     3421 tgaaatacca agggctcttc ttggaaaaag aaagcagtta tttaggcgaa ttttataaca
     3481 aaaacaaggc taatcaactg acagatgagg aatttcagct cctactggac tacaatgcct
     3541 atctttacaa gaaagctgct gagaagattt tagcaggccg gttcgccatc aatccttata
     3601 ctgaaaatgg cagaagcatt gccccatacg tccagcaaca tcaggctatt acaggctttg
     3661 aagccaatta ccatctgggc caagcccgtt tcctagaaaa attggaccta gctgatggca
     3721 agcgtctggt cggagaaaaa ctcaagcaag cttggtttga aaaaataaga gaggagttaa
     3781 atcgatgaag cttattccct ttttaagtga ggaggagatt caaaaactgc aagaagcaga
     3841 agcaaattcg agcaaggaac agaagaaaac tgccgaacaa atcgaagcta tctacacttc
     3901 tggtcagaat atcctggtct cagcatcggc tggttctgga aagacctttg tcatggcaga
     3961 gcgcattctg gaccaattgg cgcgtggtgt cgaaatttct caactcttta tctcaacctt
     4021 taccgtcaag gctgcaactg aacttaaaga acgtttagag aaaaaaatca gcaagaaaat
     4081 ccaagaaaca gatgatgtcg atctcaaaca acacttgggt cgccagttgg cagacctacc
     4141 caacgctgcc atcggaacca tggactcttt cacacaaaaa ttccttggca aacatggtta
     4201 tctgcttgat attgcaccta atttccgtat tttacaaaac caaagcgagc aacttcttct
     4261 cgaaaacgaa gtctttcatg aggtctttga agcgcattac caaggtaaac agaaagagag
     4321 ctttagtcat ttgctgaaaa actttgctgg gcgtggcaag gacgaacggg gtctgcggca
     4381 gcaagtctat aaaatctatg acttcctcca atccaccagt aatcctcaaa agtggctgag
     4441 tgaatctttc ctcaaaggct ttgagaaagc tgattttacc agtgaaaaag aaaaactgac
     4501 tgagcaaatc aaacaagccc tctgggattt ggaaagattt ttccgttacc atctggataa
     4561 cgatgccaag gagtttgcaa aggctgccta tttagaaaat gttcagttaa ttctggatga
     4621 aattggctcc ctaaatcagg agttcgatag tcaggcttat caggcagtac ttgcgcgtgt
     4681 tgtcgccatc tctaaggaga aaaacggtcg tgctctgact aatgccagcc gtaaggctga
     4741 tttgaagccc ctggctgatg cctacaacga agagagaaag acccagttta ctaaactagg
     4801 acaattatca gaccagatag cgattctcga ctatcaagaa cgttatcatg gagacacttg
     4861 gaaactagct aaaaccttcc aatctttcat gagcgatttt gtagaggctt atcgtcagag
     4921 aaaacgacag gaaaatgcct ttgaattcgc tgatatcagc cattacacca ttgagatttt
     4981 agaaaatttc ccacaagttc gtgagtctta tcaggagcgc ttccatgaag tcatggtcga
     5041 tgagtatcag gataccaacc atattcaaga acggatgctg gaattgttgt ctaatggcca
     5101 caatcgcttt atggtgggag atatcaagca atccatctat cgtttccgtc aggcagaccc
     5161 gcagattttc aatgagaaat tccaacgcta tgcgcaaaat ccccaagaag gcaagctcat
     5221 tctcctcaag gaaaatttcc gtagtagttc agaagtgctg tcagcaacca atgatgtctt
     5281 tgaacgtctc atggaccaag aggtcggcga aatcaactat gataacaagc accagcttgt
     5341 ttttgccaat accaaactga ctcccaatcc agacaacaag gcagaatttc tcctctacga
     5401 caaggacgat acaggtgagg aagaagagag tcaaacagaa acgaaactaa caggcgaaat
     5461 gcgcttagtt atcaaggaga ttctgaaact tcatcaagaa aaaggtgttg cctttaagga
     5521 aattgccctt ctgacctcca gccgcagtcg taatgaccag attctcctcg ccctgtctga
     5581 gtacgggatt cctgtcaaaa ctgacggaga gcaaaacaat tatctccaat ccctagaagt
     5641 gcaagtcatg ctagacactc ttcgtgtcat tcacaatccc ctgcaagact atgccttggt
     5701 tgcccttatg aagtctccaa tgtttggttt tgatgaggat gagctagcac gtttgtccct
     5761 tcagaaagca gaggataaag tccacgaaaa tctctatgag aaactggtca atgcacaaaa
     5821 aatggcaagt agtcaaaaag gcttgattca cacagctcta gctgaaaaac taaagcaatt
     5881 catggatatc ctagcttctt ggcgcttgta tgccaaaacc cactctctct atgacttgat
     5941 ttggaagatt tacaacgacc gtttttatta tgactatgtt ggggctttgc cgaatggtcc
     6001 tgctaggcag gccaatctct atgccctagc actgcgtgct gatcaatttg aaaagagcaa
     6061 tttcaaaggt ttgtcgcgtt ttattcgtat gattgaccaa gtcttagaag cccagcacga
     6121 tttgtcaagc gtggccgtcg caccgccaaa agatgcagta gagctcatga ccatccacaa
     6181 gagtaaaggg ctggagtttc cttacgtctt tatcctcaat atggatcaag atttcaacaa
     6241 gcaagactct atgtcagaag tcattctcag tcgtcagaat ggtcttggtg tcaaatatat
     6301 tgccaagatg gagacagggg cagtagaaga ccactatcct aaaaccatca aactctccat
     6361 tcctagtctg acctataggc agaacgaaga ggaattacag ctagcaagct attctgagca
     6421 gatgcgtttg ctgtatgttg ctatgacgcg ggctgagaaa aagctctatc ttgtcggcaa
     6481 gggttctcgt gaaaagctgg aagtcaagca atacccagca gccaaaaatg ggaaactaaa
     6541 tagcaatact agactgcaag cacggaattt ccaagattgg ctttgggcta tcagtaaagt
     6601 gtttactaag gacaagctca actttagtta tcgttttatt ggcgaagatc agttgaccag
     6661 agaagctatc ggagagttgg aaaccaagag tcctctccaa gatagctccc aagcagacaa
     6721 tcgtcagtca gataccatca aagaagctct ggaaatgctg aaggaggtgg aagtttataa
     6781 tactcttcac cgcgcagcta ttgaacttcc tagtgttcaa accccaagtc aaatcaagaa
     6841 attctacgaa ccagttatgg atatggaagg tgtcgagatt gctggtcaag gtcagtcagt
     6901 aggcaagaaa atcagcttcg atttgccaga tttttcaacc aaagaaaagg taactggagc
     6961 tgagattggt agtgctactc acgaactcat gcagagaatt gacctcagcc agcaactaac
     7021 ccttgctagc ctaacagaaa cactcaaaca agttcaaact agccaagctg tcagagacaa
     7081 gatcaatctt gataaaattc ttgctttctt tgacacagta ctcggtcagg aaattcttgc
     7141 taataccgac catctctatc gcgagcaacc tttctccatg ctcaaacgag accaaaagag
     7201 tcaggaagac tttgttgtcc gtggtatcct tgatggctat ctgctttacg aaaacaaaat
     7261 tgttctgttc gactacaaga cagaccgcta tgatgaacca agtcaactcg tagaccgcta
     7321 tcgtggtcag ttagctctat acgaagaggc tttatcacga gcctattcga ttgaaaatat
     7381 tgaaaaatac ttgattttac tcggtaaaga cgaggttcaa gttgtaaaag tataacttat
     7441 actctttgaa aatctcttca aaccacgtca gcttcgcctt gctgt
//
LOCUS       DAVBNO010000073         7474 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVBNO010000073 DAVBNO010000000
VERSION     DAVBNO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.073"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            52..693
                     /locus_tag="VQ846_001876"
     CDS             52..693
                     /locus_tag="VQ846_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET3076406.1"
                     /translation="MRAGVLGANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAGA
                     FSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLDAAYIQNGECKTSAQLLT
                     NKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYRI
                     PATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(735..2723)
                     /locus_tag="VQ846_001877"
     CDS             complement(735..2723)
                     /locus_tag="VQ846_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3076407.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(2725..3483)
                     /locus_tag="VQ846_001878"
     CDS             complement(2725..3483)
                     /locus_tag="VQ846_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3076408.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            3911..4102
                     /locus_tag="VQ846_001879"
     CDS             3911..4102
                     /locus_tag="VQ846_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076409.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(4456..4728)
                     /locus_tag="VQ846_001880"
     CDS             complement(4456..4728)
                     /locus_tag="VQ846_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET3076410.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(4787..5236)
                     /locus_tag="VQ846_001881"
     CDS             complement(4787..5236)
                     /locus_tag="VQ846_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET3076411.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTQRYRCGLCRGKLLLINQPED"
     gene            complement(5223..7352)
                     /locus_tag="VQ846_001882"
     CDS             complement(5223..7352)
                     /locus_tag="VQ846_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET3076412.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVSVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
BASE COUNT         2210 a         1562 c         1472 g         2230 t
ORIGIN      
        1 tcagaaacaa atcacgaaat cgattcaaat tttgcaggtc gtttaaatat cctgcgcgca
       61 ggtgttcttg gtgctaacga tggaattatt tccattgctg gtgtggttat cggggttgcc
      121 agtgccacga ccaatatctg gattatcttt ttatcaggat ttgcggctat cttagctggt
      181 gccttttcaa tggctggtgg agaatatgta tccgtttcaa ctccaaaaga taccgaggaa
      241 gctgccgttt cgcgagaaaa actcttgtta gaccaagata gggaactagc caaaaaatcc
      301 ctcgatgctg cttatatcca aaatggagaa tgcaaaactt ctgcccaact cttgaccaat
      361 aagatctttc ttaaaaatcc actcaaggct ctggtagagg aaaaatatgg gattgagtat
      421 gaagaattta ccaatccttg gcacgctgcc atttctagct tcgttgcctt tttccttaga
      481 agtttgcctc caatgctgtc agtgaccatt tttccaagtg attaccgcat ccctgctact
      541 gtccttattg tcggtgtggc ccttcttctc actggttaca ctagtgccag acttggaaag
      601 gatccgacta gaacagctat gattcggaac cttgctattg gtctcttgac catgggagtt
      661 accttcctgc tcgaacaact tttcagcatt tagaatacaa gaaatacctc gattttgaag
      721 tcgaggtatc ttttttacat ttgcacaatc ttgcgataac ttcttgaagt aatcatgaaa
      781 atcagcacat aggcgatgag gaagatagcg cagatagaca aggtcacaat caacatcata
      841 gtcgtatcca gtacaccaat cacttttaaa atcaggctaa gcatatggta ggcaaaggcg
      901 agatgtatga aggcaaagag caaaggaagg aagaaaacag ttaaaacctg tttgttgatg
      961 gtttgcttga tttgcttttg gtccaaacca actttctgca agataataaa gcgttcacgg
     1021 tcttcgtagc cttcagaaat ttgtttgtag tagatgacca gaacagttcc gaccataaag
     1081 ataatggata ggaaaatacc gataaagaag acaccaccaa agagggcact catctgagaa
     1141 ctagcatctg ctagattgct accataaaca tagctacctt ctgtgtctaa ttgagcatta
     1201 aattgattga ggtagttttc atactcctca gcgaccttga gttgttcttc ttcgctggca
     1261 tctacattca taccaccgta aaactgatta tagatagccg aatctgggaa ttgatccaaa
     1321 aaggcttgta aatcaggaac aacaaggtaa ttgtaatcag tagtcaagat attaaactta
     1381 tttggaacat ggttcacaat gaaatcttta ttaaattctt ctttgacaga aaattgatga
     1441 tcatttagag ttagagcttt ctgtcctttc agtccgtcat ttttggcaaa gagaccgacc
     1501 tcatttcctg atagagacag tttttgacca gtcatatttt cataatcttt ttggtcaaat
     1561 accatgaaaa ctgtttttgg ttggacacgg ttttgtcctt tttcaaaaat agttaacttg
     1621 gttccttctt gattcgcaac agcaaagtaa gtgtaacgaa gcacttcttt ctctttaatc
     1681 ttataacctt tgtcacttgc aaactggctc aagagtttgt ccaaatcttc tttttcaaca
     1741 ttttgccctg aaaccccaaa atcatgagga tttagaactt ttttaaagga ttctgcggaa
     1801 ttgaaaatgc ttgtcgctgc tgacatggtt accaaaacca ttgttgacaa aatagcgata
     1861 gttgctagtc caactgcatt tttcttcata cggaaaatca agttagaaac agatatgaga
     1921 ttattaggtt ggtaatagta tttcttattt ttctttaaga tttgaaggaa aacggtaatc
     1981 cctgcattga acaaaagata agtaccaaag ataaccagta aaacagctat gaagaaggtt
     2041 gttaaggctg taagaggatc ttttaccgta agggcaagat aatatccaat ccctaaactt
     2101 atggaaccaa gaatagtttg gagaggaagg aagcgacctt ttttctctcc gctagctttc
     2161 tcacgtgaga gctggagggc attcatacgg gcgattcgaa gggcattcag gaacatgagg
     2221 cctaggaaaa tcaaaccgaa gacaagaagt actgtaatga caactttcat ctggaaggta
     2281 gcgaccagtt ccaccttcaa tttcatcagt ttgagtagga aagcgaaaat caacttgtca
     2341 aacaaggctc caataccgat tcctgctcca acagttagaa tcccaaatag cactaactcc
     2401 ttaaaggtca tactgattag atggcgcttc tccaagccta acatgccata tatacccagt
     2461 tccttggaac ggtttttcat gacaaaacta ttggcataga ggacgataat ggctgacgca
     2521 agggtaacga caaacatacc aaatccaagt gttgcttgaa tggtggttcc tccacggatt
     2581 tccgcaatct ttggattgaa ggttagggag taaaagagat aggtgatggt gactgccaag
     2641 agaacagcca gtgcaaaggg atagtagagt ttgcggtttt taatcaagtt cgataccgct
     2701 aacttattgg ttaatcgaaa catactaatt cacctcgctt gccatgacag tcaaggtatc
     2761 agagatttct tggaacatct gacgttctgt cttctctcca cggtagattt gattgtaaag
     2821 aatgccgtct ttgataaaga gaacacgctt ggccctgcta gcagctgctg ttgagtgggt
     2881 taccatgagg atggtttgcc cacgctcatt gatttcatta aagacatcaa gtaaggctgc
     2941 agatgacttg gaatcaaggg ctcctgttgg ctcgtccgca aggagaattt caggttctgt
     3001 gatgatggcg cgggctactg ctacacgctg tttctgacca ccagaaatct cgtaagggta
     3061 cttctcttgc aattggttga tacccagatt ttcagctgtc atcaccaatt tcttcatcat
     3121 ctccgttata ggtcttcttg acaagacaag cggaagcaag atattgtcct taacagacag
     3181 agtatctagc aagttaaagt cttggaagac aaatcctagc ttttcacgcc ggaaactaga
     3241 agcctgtgaa tttttaatag ttgctgtatc agttccgttt aggtaaacct gcccacgact
     3301 tggtttatcc agcatagcta ggatattgag cagagtggat ttaccagaac cagactcacc
     3361 catgatagca acgtagtcac ccttttctac ggtgaagtga atatccttga gggcctctac
     3421 ttggttgccc tgaaaacgtg ttttataaat tttttgaacg tgttttacat ctaaaagtgt
     3481 catgagaatc tcctttctta atatcccatc aaatgaaatg tagcttgcat catagtgcat
     3541 cccagctgaa cgcgctcaat ttctttgtga cttggttttc tagttttctt atgtaagatt
     3601 tgtttcatga tgttactcct ttgttcttta tgagtctagt ttacatcaaa aaaagaccgc
     3661 ttgccataac ctaaccttac aaaagagact ttaatcttac atttttgtaa gattgggtga
     3721 aatataagca aaattactta gaaaatgatt tgatttccta tttttaaagt tgtaaaatat
     3781 aagtgtgaaa aggcttactt tgaaaaggag gaagcgggaa ctaacatttt agtaaggtta
     3841 cctagataac ataccattca atttaacact tgcaagtcgt tcaacctata aggaaagaag
     3901 gtgcgcttta atgaaacaca aagaacatat actcatcgga cttctctatc tgctctctcc
     3961 attcatcggt cagctcttag ttgaacacac acactttatc ggtacagaat ttaccggcac
     4021 tgcttatgtt atttgctggt tatcagttgt tatcagcatc caccattttt ccaaaaatgt
     4081 attgtctcag cagcaaaaat agattaaaaa actgagttta aattactgct tttataataa
     4141 aatgaaataa gaacaggaca aatcgatcag gatagtcaaa tcgatttcta acaatgtttt
     4201 agaagtagag gtgtactatt ctagttccaa tccactatag tttgtggatg aagctggcga
     4261 aatattatta atactattca aaaatcaaag tgcaaactat gtcaacttca cattgccgac
     4321 taaattacag cctgtggctg gcttcctagt ttgcactttg atttttattg agtataaaag
     4381 taaaaaagct ttaaatcaag cagtttagac tattgattta aagatttttg cttattccaa
     4441 atcttctgta gaacctcacc aaaaccagcc ttcattgtcg tcaatggctt gggcttcttt
     4501 gcgtttacgt gggcctgttc cgtacatttc tgcttcgatt tcttccttgg taggcatgat
     4561 agaagctagg atgatataga taatcacgcc aagtccaaag tttgcaactg taaaaatggc
     4621 aaacaagaaa cgaactaggg taacatcaaa attccacttg tctgacagac ctgccagaac
     4681 tcctgaaatc atgcgattgc gtctcatttt ataaaattta ctgttcatga tatttcctct
     4741 ttctgctagg ttagacatag tatatcacgg ggaggctcgg ctttcatcag tcctcaggct
     4801 gatttatcag aagcaattta cctcgacaaa gtccacagcg atagcgttgg gtatcaatcc
     4861 tacgcttgcg ctgatacctt tgctggcagg attgacaacg ataaagcttg agtggtttag
     4921 agttactatt gggcaaggat ggtacaaacc ttaatccatc cactgctttc aaaagttcct
     4981 taaaatcccg atccttgtgt tgatagccct tcccttggaa ataaaggtga taatgacaga
     5041 gttcatgtcg gacaattttc ctaaaaacct ccatacccag tttctgataa accttgggat
     5101 taaaatccag atgcccatct ttggggaaaa atcgcccacc tgtcgaacgt agacgcctat
     5161 tccactggac atgatggata aaagctctcc cgaagtcttc tagtgaaacc tgcttgacat
     5221 aatcagtcag tttcatttgg agcgaggagc gacagattaa ctttttcacg ttcagtatca
     5281 attttcttga cccaaacggt caccaaatct ccaactgaca ccacttgact agggtgtttg
     5341 ataaacttgc gactcatatg agaaatatga atcaagccgt cctcgtgaat cccgatatca
     5401 acgaaggcac cgaagtcaac aacgttacgc acaacacctt ctagcttctg acctaccact
     5461 aggtctttga tatctaggac atcttggcga agcacaggtg cgtcaaagga atcacggaaa
     5521 tctcgacctg gtttgagaag atctgcaatg atatctttaa gggtttctgg accgaggtct
     5581 agctcttgag ccatttcctt gactgaaagg gacttgagtt tgctttgggc ttcttcgttt
     5641 aggtctttaa tatctaaacg tttgaagagt tccttaactg cagtgtaatt ctctgggtga
     5701 actcctgtat tatcaaggat attgctactt tcagggatac gaaggaaacc agcagcctgc
     5761 tcaaaggcct tggctcccag acgaggaact ttcttgattt gggcgcgtga agtgattttt
     5821 ccttcttcct cgcggtattt gacaatattt tcagagatag ttttgttgag tccagctacg
     5881 tgtgaaagaa gagctgggct agctgtattg acattgacac caacttggtt aaccactgta
     5941 tcgacaacaa agtccagact ctcagatagt ttcttctgac tgacatcgtg ttggtattga
     6001 ccgacaccaa ttgacttagg atcgattttg accaattccg caagaggatc ttgcaaacga
     6061 cgggcgatag aaatggcaga gcgtttttca acggtcaagt ctggaaactc ctgacgagca
     6121 agttcgctgg cagaatagac agaagcacca ctttcattaa cgataacata gctgacttca
     6181 gggaaatctt tcagaacttc cgctacaaaa gcttcacttt cacgactggc cgttccattt
     6241 ccaatggcaa taatctctac accgtattga ccaattaaat ctgctaaatc tttcttggct
     6301 tcttcgattt gacgagctga tgctggttta acaggataaa taacctgagt tgtcagcatt
     6361 tttcctgttg catccacgac agctaacttg gcacctgtac gaaaggctgg gtcaaatcca
     6421 agaaccacgc gccctttcag tggagcaacc aagaggagat tgcgcagatt gtcagaaaaa
     6481 agttggatag ctccttcttc agctttctca gttaattctg tccgaatacg acgctcgata
     6541 gcaggcaaga cctttttctt aacggattgc tgaacaactt catcaatata agcatttttc
     6601 accttgaaac gagtagcaaa gaaggcaaga atacggtccg tcgcatgttc aaaaccgatc
     6661 ttcaagacac caagtttctc cccacgattg agagccaagg tacgatagcc ttgcatagtt
     6721 ccaactgtct ctgaaaaatc ataataaatc tgaaaaacct gcttttcatc aagactttca
     6781 tccttggctt gagaagtgag tttagagtgt ctcagcactt cctgataagt catagaacgc
     6841 aaggtcacat cttccgataa ggcttcgacc aaaatatcaa ctgcaccggt caaggcttcc
     6901 ttgccagtcg caaatccttc acagacgaac ttttcagctt ctttctctaa gtcaactata
     6961 ttctgcaaaa tcaagcgagc aagaggaaag agtccagctt cacgggcaat ggttgccttg
     7021 gtacgacgct tttccttata aggaagatag agttcttcaa cgtctgctaa tttttcggca
     7081 actaagatag cttcttccaa ttccttggtc aacttacctt gttcttgaat cttagctaag
     7141 acagcttcct tacggtcatt gagatttgtc agacttttat ccaaatcaat aatagcctta
     7201 atcgccacct catccagact accagtcatg tccttgcgat aacgcgcgat aaagggaata
     7261 gtcgcccctt cagctgtcaa acttagaacg gtatcaattt gctttaacgt cactcccaaa
     7321 tcctgagaga ttttttcata ttttttatcc ataaatctat tataccacaa gctggaaggc
     7381 tttctccaat ccttttcata ctcaataaaa atcaaaaagc aaactaggaa gctagccgta
     7441 ggctgctcaa aatactgttt tgaggttgtg gata
//
LOCUS       DAVBNO010000074         7458 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVBNO010000074 DAVBNO010000000
VERSION     DAVBNO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.074"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..116
                     /locus_tag="VQ846_001883"
     CDS             <1..116
                     /locus_tag="VQ846_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET3076413.1"
                     /translation="TPEQAKQILEYGVRGIVVGGAITRPKEITERFVASLK"
     gene            135..1652
                     /locus_tag="VQ846_001884"
     CDS             135..1652
                     /locus_tag="VQ846_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET3076414.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            1777..3105
                     /locus_tag="VQ846_001885"
     CDS             1777..3105
                     /locus_tag="VQ846_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET3076415.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            3358..4224
                     /locus_tag="VQ846_001886"
     CDS             3358..4224
                     /locus_tag="VQ846_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET3076416.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            4240..5079
                     /locus_tag="VQ846_001887"
     CDS             4240..5079
                     /locus_tag="VQ846_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET3076417.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            5351..5803
                     /locus_tag="VQ846_001888"
     CDS             5351..5803
                     /locus_tag="VQ846_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET3076418.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            5900..6244
                     /locus_tag="VQ846_001889"
     CDS             5900..6244
                     /locus_tag="VQ846_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076419.1"
                     /translation="MVSSKHAARASFLDGQGITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGISIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            6528..7172
                     /locus_tag="VQ846_001890"
     CDS             6528..7172
                     /locus_tag="VQ846_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET3076420.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLLELGIVSLSLLDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLTWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            7341..>7458
                     /locus_tag="VQ846_001891"
     CDS             7341..>7458
                     /locus_tag="VQ846_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET3076421.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDK"
BASE COUNT         2290 a         1322 c         1519 g         2327 t
ORIGIN      
        1 atacaccaga acaagccaaa caaatccttg aatatggagt gcgaggcatc gttgttggtg
       61 gcgccattac tagaccaaaa gagattacag aacgcttcgt tgctagtctt aaataagatg
      121 tgagagggag ttttatgttt aaagttttac aaaaagttgg aaaagctttt atgttaccta
      181 tagctatact tcctgcagca ggtctacttt tggggattgg tggtgcactt tcaaacccaa
      241 ccacgatagc aacttatcca atactagaca atagtatttt tcaatcaata ttccaagtaa
      301 tgagctctgc aggagaggtt gtattcagta atttgtcact acttctctgt gtgggattat
      361 gtattggctt agcgaaacga gataaaggaa ccgctgcgtt agcaggagta actggttact
      421 tagttatgac tgcaacgatc aaagctttgg taaaactttt tatggcagaa ggatctgcaa
      481 ttgatactgg agttattgga gcattagttg tcggaatagt tgccgtatat ttgcacaacc
      541 gatataacaa tattcaatta ccttccgctt taggattctt tggaggttca cgcttcgttc
      601 ctattgttac atcgttctct tctatcttga ttggctttgt cttctttgtt atttggccac
      661 ctttccaaca acttcttgtt tctacaggtg gatatatttc tcaggcgggt ccaattggaa
      721 cttttctata tggattttta atgagacttt ctggagcagt aggcttacat catataattt
      781 accctatgtt ttggtatact gaacttggtg gtgttgagac tgttgcagga caaacagtgg
      841 ttggagctca aaaaatattt tttgctcaat tagccgatcc ggcccattct ggattattta
      901 cagaaggaac aaggtttttt gcaggtcgtt tctcaacaat gatgttcggt ttaccggctg
      961 cctgtttagc gatgtaccat agtgttccta aaaatcgtcg taaaaaatac gcgggtttgt
     1021 tttttggagt tgctttaaca tcttttatta ccggtattac agaaccaatt gaatttatgt
     1081 ttctattcgt cagtccggtt ctatatgttg ttcacgcatt ccttgatggt gttagcttct
     1141 ttattgcaga cgtcttaaat atttcaatag gaaacacatt ttcaggaggt gtaatcgatt
     1201 tcactttatt tggaattttg caggggaacg ctaagacgaa ttgggttctt cagattccaa
     1261 ttggacttat ttggagtgtt ttgtattata ttatttttag atggttcatt actcaattca
     1321 acgttctaac gccaggacga ggagaagaag tagattctaa agaaatttct gaatccgcag
     1381 attcaacttc aaatactgca gattatttaa aacaggatag cctacaaatt atcagagcct
     1441 tgggtggatc aaataatata gaagatgtag atgcttgtgt gacacgttta cgtgtagctg
     1501 taaaagaagt taatcaagtt gataaagcac ttttaaaaca aattggtgca gttgatgtct
     1561 tagaagtgaa gggtggcatt caagcaatct atggagcaaa agcaatctta tataaaaata
     1621 gtattaatga aattttaggt gtagatgatt aagtacttac tgacttaata aaaaacagag
     1681 gagagtgatg gatgagtagg atgaaatgaa atcgcataca agaaataaag aactcattat
     1741 ccaagttgga tacgcttatt acataggaga atacaaatga aatttagaaa attagcttgt
     1801 acagtacttg cgggtgctgc ggttcttggt cttgctgctt gtggcaattc tggcggaagt
     1861 aaagatgctg ccaaatcagg tggtgacggt gccaaaacag aaatcacttg gtgggcattc
     1921 ccagtattta cccaagaaaa aactggtgac ggtgttggaa cttatgaaaa atcaatcatc
     1981 gaagcgtttg aaaaagcaaa cccagatata aaagtgaaat tggaaaccat cgacttcaag
     2041 tcaggtcctg aaaaaatcac aacagccatc gaagcaggaa cagctccaga cgtactcttt
     2101 gatgcaccag gacgtatcat ccaatacggt aaaaacggta aattggctga gttgaacgac
     2161 ctcttcacag atgaatttgt taaagatgtc aacaatgaaa acatcgtaca agcaagtaaa
     2221 gctggagaca aggcttatat gtatccgatt agttctgccc cattctacat ggcaatgaac
     2281 aagaaaatgt tagaagatgc tggagtagca aaccttgtaa aagaaggttg gacaactgat
     2341 gattttgaaa aagtattgaa agcacttaaa gacaagggtt acacaccagg ttcattgttc
     2401 agttctggtc aagggggaga ccaaggaaca cgtgccttta tctctaacct ttatagcggt
     2461 tctgtaacag atgaaaaagt tagcaaatat acaactgatg atcctaaatt cgtcaaaggt
     2521 cttgaaaaag caactagctg gattaaagac aatttgatca ataatggttc acaatttgac
     2581 ggtggggcag atatccaaaa ctttgccaac ggtcaaacat cttacacaat cctttgggca
     2641 ccagctcaaa atggtatcca agctaaactt ttagaagcaa gtaaggtaga agtggtagaa
     2701 gtaccattcc catcagacga aggtaagcca gctcttgaat accttgtaaa cggatttgca
     2761 gtattcaaca ataaagacga caagaaagtc gctgcatcta agaaattcat ccagtttatc
     2821 gcagatgaca aagagtgggg acctaaagac gtagttcgta caggtgcttt cccagtccgt
     2881 acttcatttg gaaaacttta tgaagacaaa cgcatggaaa caatcagcgg ctggactcaa
     2941 tactactcac catactacaa cactattgat ggatttgctg aaatgagaac gctttggttc
     3001 ccaatgttgc aatctgtatc aaatggtgac gaaaaaccag cggatgcttt gaaagccttc
     3061 actgaaaaag caaacgaaac aatcaaaaaa gctatgaaac aatagtcctt agttattcta
     3121 tgaaaaagta attttttaaa gaacctaaga gtgtataccc ccttttccct ctacacagat
     3181 agtgtaagaa aagggggctt ttgtttaaaa tgtaagaaac tgtcacgaaa ttaaaatgaa
     3241 gttcttacat aagcgaatca taaaaaattt cattttgatt ttaaaacagt tcaagaaagt
     3301 caaaaaatta ttctatttga aagagaggtg ccgactgtga aagtcaataa aatccgtatg
     3361 cgggaaacag tgatttccta cgctttccta gcaccagtat tattcttctt tgtcatcttt
     3421 gtgttggctc cgatggtgat gggcttcatt acaagtttct ttaactactc aatgactaaa
     3481 tttgagtttg taggcttgga taactatatc cgtatgttta aagatcctgt ctttacaaaa
     3541 tctctgatta acacagttat tttggttatt ggatctgtac cagttgttgt tctattctca
     3601 ctctttgtag catctcagac ctatcatcaa aatgtcattg ccagatcctt ctaccgtttc
     3661 gtcttcttcc ttcctgttgt aacgggtagt gttgccgtga cagttgtttg gaaatggatt
     3721 tatgacccac tatcagggat tctaaacttt gtccttaagt caagccacat catcagccaa
     3781 aacatttctt ggttgggaga taaaaactgg gcattgatgg cgattatgat tattctcttg
     3841 accacttcag ttggtcagcc catcatcctt tatatcgctg ccatggggaa tattgacaat
     3901 tcactggttg aagcggcgcg tgttgatggt gcaactgagt ttcaagtttt ctggaagatt
     3961 aaatggccaa gccttcttcc aacaactctt tatattgcaa tcatcacaac aattaactca
     4021 ttccagtgtt tcgccttgat tcagcttttg acatctggtg gtccaaacta ctcaacaagt
     4081 accttgatgt actaccttta cgaaaaagcc ttccaattga cagaatacgg ctatgccaac
     4141 acaattggtg tcttcttggc agtcatgatt gctatcgtaa gctttgttca atttaaagta
     4201 cttggaaacg acgtagaata ctaaagaaag gagacagcta tgcaatctac agaaaaaaaa
     4261 ccattaacag cctttactgt tatttcaaca atcattttgc tcttgttgac tgtgctgttc
     4321 atctttccat tctactggat tttgacaggg gcattcaaat cacaacctga tacaattgtt
     4381 attcctcctc agtggttccc taaaatgcca accatggaaa acttccaaca actcatggtg
     4441 cagaaccctg ccttgcaatg gatgtggaac tcagtattta tctcattggt aaccatgttc
     4501 ttagtttgtg caacctcatc tctagcaggt tatgtattgg ctaaaaaacg tttctatggt
     4561 caacgcattc tatttgctat ctttatcgct gctatggcgc ttccaaaaca agttgtcctt
     4621 gtaccattgg tacgtatcgt caacttcatg ggaatccacg atactctctg ggcagttatc
     4681 ttgcctttga ttggatggcc attcggtgtc ttcctcatga aacaatttag tgaaaatatt
     4741 cctacagagt tgcttgaatc agctaaaatc gacggttgtg gtgagattcg taccttctgg
     4801 agtgtagcct tcccgattgt gaaaccaggg tttgcagccc ttgcaatctt taccttcatc
     4861 aatacttgga atgactactt catgcaattg gtaatgttga cttcacgtaa caatttgacc
     4921 atctcacttg gggttgcgac catgcaggct gaaatggcaa ccaactatgg tttgattatg
     4981 gcaggagctg cccttgctgc tgttccaatc gtcacagtct tcctagtctt ccaaaaatcc
     5041 ttcacacagg gtattactat gggagcggtc aaaggataat actctgagaa aatctcttca
     5101 aactacgtca gcgtcacctt gccatactta agtattgcct gcggttagct tcctagtttg
     5161 ttcttcaatt ttcattgagt ataggaaaat caatctatca agatacagaa gtatatttta
     5221 tagatttaga gaatatagag gttataagtg tctacaaaat ggagggtatg cagttacttt
     5281 atgaagtttt gtcagacact tataaactta agaatggttt tagttaacta tcagaaacga
     5341 aggaaagagt atgatttttg acgatttgaa aaacatcacc ttttacaaag ggattcatcc
     5401 caatttagac aaggctatcg actatctcta ccaacatcgt aaagattcat tcgaattagg
     5461 aaagtatgag attgatggag ataaagtctt tctagttgtt caggaaaatg tcctcaatca
     5521 agttgagaat aatcaatttg aacaccataa gaactatgca gatttgcatt tgctggtaga
     5581 aggacatgaa tattcgagct acggttcacg tatcaaagac gaggcagtag cattcgacga
     5641 agcgactgac attggctttg ttcattgtca tgaacactac ccactcttgt tgggttatca
     5701 taattttgcg attttcttcc caggtgagcc acatcagcca actggctatg caggtatgga
     5761 agaaaaggtt cgaaaatatc tctttaaaat tttgattgat taaaaatagg atgaattgtt
     5821 tttttgtaaa gctttgacaa tactctacca tgaaattgat ctttgtgagg tagagaaatg
     5881 agaataaaat atttaaaaat tggtatcttc taagcatgct gcaagagcta gtttcttaga
     5941 tggacagggg attacagttg atgagatgtc ctggataatt aatggcattg tgaatgcatt
     6001 gattggtaga tacataaaat taggtactta tgcagctaag tatggtatta gtatagcacg
     6061 ctcgatctta agtagggcag ctgcaactgc agcagcaaga gtaggattac tgaccaagat
     6121 ttctggatgg attttacgag tagctgtgaa tgtagctgat gtatatggta attttgccaa
     6181 caatattgct gcagcttggg atgcatatga taaaattcct aacaatggtc gtataaactt
     6241 ttaaaatgcg agaatgaaag cactttgtat ttttttattg aatatgttag cttggacagt
     6301 gcttgcaatg ataattcgtg gagggctaga tggatttgat aggcatactt ggagtactat
     6361 tttaattgct tcgctgttcg gggtatatga ttataagccc atagataaaa atagaaaaaa
     6421 gtccaaaaga aaaaagaaaa aatagatttg ttcatggtag ggacttatga aagctttact
     6481 gacaaaaaag aaaacagttt acaaagaaaa atgatggagg agcaaacatg gcacaaaaag
     6541 gagtaagcct tatcaaggca gcatttgata cagataactt tctcatgcgt tttagtgaga
     6601 aggtcttgga catcgtgaca gccaatcttc tttttgtcgt ctcttgtttg cccatcgtga
     6661 cgattggagt ggctaaaatc agcctctacg agaccatgtt cgaagttaag aagagcagac
     6721 gggtgcctgt ttttaaaatc tatctaagat ctttcaagca aaatctgaaa ctaggtcttc
     6781 agttaggtct gcttgagttg ggcattgttt cattaagcct cctagatctt tatctctttt
     6841 ggggtcaaac agctctgccc tttcaattgc tgaaagctat ttgtctaggc attctgattt
     6901 tccttacgat tgtgatgttg gctagctatc caatcgcggc acgttatgat ttaacttgga
     6961 aagaaattct tcaaaaagga ttgatgttgg ctagttttaa ctttccttgg ttcttcctca
     7021 tgttagccat tcttgtcctc attgtgatgg ttctttatct gtctgccttc agtctactct
     7081 taggtggctc agtcttcctg ctttttgggt ttggactatt ggtctttatc cagactggat
     7141 tgatggagaa aattttcgca aaataccaat aggagcctta tttctgaaac tactttcaaa
     7201 ggctccaaac gctattctat aagcgagaaa ctaaaatcta agtgtctata agatattgaa
     7261 agccttttcg gtaaagtgta tactaaagaa gtaaagaaaa atctgcttta gcagaaaata
     7321 aaaataatag gagaaaatct atgtcagatt tgaaaaaata cgaaggtgtc attccagcct
     7381 tctacgcatg ttatgatgag caaggagaag taagtccaga gcgtacgcgt gccttggttc
     7441 aatacttcat tgataaag
//
LOCUS       DAVBNO010000075         7455 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVBNO010000075 DAVBNO010000000
VERSION     DAVBNO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.075"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(173..1588)
                     /locus_tag="VQ846_001892"
     CDS             complement(173..1588)
                     /locus_tag="VQ846_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET3076422.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1601..3439)
                     /locus_tag="VQ846_001893"
     CDS             complement(1601..3439)
                     /locus_tag="VQ846_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET3076423.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVIPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     DVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3457..4296)
                     /locus_tag="VQ846_001894"
     CDS             complement(3457..4296)
                     /locus_tag="VQ846_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET3076424.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(4597..7276)
                     /locus_tag="VQ846_001895"
                     /pseudo
     CDS             complement(4597..7276)
                     /locus_tag="VQ846_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         2446 a         1569 c         1025 g         2415 t
ORIGIN      
        1 ccaatctatc ctaagttaac tatttataat cggctctctg taacaaaatt aaattaatta
       61 atccccctat aaaataaaga ggtttagaat ttgatgtctt ccaaacctct ttatttcata
      121 tttaatgaga tgctgcctta accgtgataa tatctaatca tcaataaaca aactatttga
      181 ataaggattc tccatttgat tcaatcactc ctttatacca agtaaaggac attttcttat
      241 atcgattgaa tgtgccattt ccatcatcat ttcgatcaac ataaatgaga ccgtacctct
      301 tagaaagctg tgcagtggac atagaaacgc agtcaataca tccccaagac gtatagccca
      361 taatttccac accatcttgt agagcttcag ctacttgcaa taaatgttct ttcatatact
      421 gaattctata atcatcttgg actgttaagt tattaaattc atcttttatt agttgatctt
      481 tagcacctaa tccgttttcc actataaata atggaatttg ataacggtca taatatctat
      541 ttaaaattat acgtagtcca attggatcaa tttgccatcc ccactcagaa gactctaaat
      601 aaggatttac taaaccacca ataatattcc cttctcctga attatactgt gttggaagag
      661 cagattgagt cacactcatg taatagctaa aggataaaaa atctacagta taatttttta
      721 ataactctgc atcttcagct gcaaactcta tgttaatgtc attttcctta aaatatcttt
      781 ttgcataatt cggataataa cctctaacat gcacatctga aaatagataa tttagattct
      841 catactcatg agtcgcccat acatcttttg gatttggagt cattggataa gctggcatag
      901 ctaataccat acaccccacc ttaaactctg aattaatctc acgagcaatt tttgtaacca
      961 aactcgaggc gactaattca tgatgtatag cttgatataa ttcttgtttc gaaagattct
     1021 ccttaggtat atctattcct ccactagtaa acggtaattc caaaacagag tttacttcgt
     1081 taaatgtaag ccaatattta actttatctt tatacctttc taaaactgtt cgagcaaatt
     1141 tttcataaaa atgaatcatt ctcctatcaa tccatccatg atattttctt gctaaatata
     1201 atggagtctc atagtgtgaa agagttacaa gtggttctat cccgtgagca tgtagttcat
     1261 caaacaattc atcataatat ttcaacccag cttcgttagg ttcttcctca tctccttttg
     1321 gaaaaattct actccatgca atagaagtac gaaaaacatt aaagcccatt tcagaaaaca
     1381 aggatatatc ttccttatat ttatgataaa aatcaatacc tatcaatttt aagttatctt
     1441 ctgtaggatt ttctgttgct tctcctaatc cacctttggg taacacatcc tgaactgata
     1501 agcccttacc atcttcatta tatgctccct ctacttgatt agctgcaaca gctccacccc
     1561 aaagaaaatc atctggaaaa atggtcataa ctttcctcca ttataatatt accagtaatt
     1621 ccttagaatg ctcgattgtc tgattattag gtaatactaa tacatctaga aaatcattgg
     1681 tattcgttac aattactggt gtaactgttt cgtagccttt agtcttgatt aaattcaagt
     1741 ccatttcaaa aatcaactga tttttgaaaa ctctgtctcc ttcttctaca tgactaataa
     1801 aaccttgacc ttttagctca acagtatcta atccaatatg aattagtaac tcaacaccct
     1861 catcactctt caatccaatt gcgtgcttag tcggaaaaat atttgtaatt ttcccatcaa
     1921 atggtgcata aaccttacct tcacttggaa taatcgctac tccgtctcca attagtttat
     1981 ctgaaaatgt tttatcctgg acatcgctta acggaatgat ttctcctgat ataggagaaa
     2041 atatcatttt tttatttgaa actccagctt taacttctaa attgctagaa ctctcttctt
     2101 catcgattcc aaatatataa gctaatacaa aggtaataac aaccgaaata accgccacaa
     2161 ttaaagcatt tacaatattt gatggtacat cagaataaat aaattgaggc aacgctatca
     2221 aagatgggac agcaaataga tacgctttaa cactagtaag acctgcaaat aatcccgcta
     2281 atccaccacc aatcatagct gcataaagcg gttttttata ttttaaagtc acaccatata
     2341 atgcaggttc ggtaatccct gcaagtaagg ctgagaaacc tgctgcaaaa gcaatttgtt
     2401 ttgtattatt atttttactc tttaatgcaa cagccatcga agcagcccct tgagctaagt
     2461 ttgaccctaa cattgctgga agaattaata cgtctggagt agcaatagat gccgccaaaa
     2521 aaataggtgc aaaagcccaa tgcattccag tcataacaat aaatggcata atagcaccaa
     2581 gaatagctaa tgtaagccat ccagctacat catacatttg cccaactaga tttgataatc
     2641 cttcaccaac aattactcca ataggcccga ctacaactaa ggcaatacag cttgatacta
     2701 ataatactag cgtaggttgc aaaaaactct tagtaacagc tggtgttaat ttagcaatca
     2761 ttttttcaat atatttcatc aaccaaacca taataagaat tggaacgact gacgaaccat
     2821 aactagctgg tatcacaggt acaccaaata aactaagagg attccctgat tgcaccattt
     2881 gaacaaaatt tggatggaga agtacacctg ctacagacat agctaatgta gatgttactt
     2941 ttaatttttg tgatgcagaa taagctaata acagcggtaa gaaataatat ggagcatccc
     3001 caaaaaatgt caaaaaagca atagtctgag aatctgattg caatatacca agcattggta
     3061 aaataattac caagactttc agcatacctc cccctaacat tgctggaatg attggagtca
     3121 tggaaccagc gatatactca atgattcttt ctaaaatatt ccctttgtgc ccttgaacaa
     3181 ctgaatcgga ttcaaaattg ccaagtttaa cgaattcttt ataataatta gctacatcat
     3241 taccaagtat aatttgatat tgtccattct ttttcataat acctattaca cctggtatct
     3301 tcttcacatc atcatcattg actaaatttt catcttttaa ttctaatctt aaacgtgtta
     3361 cacaatgggt aactctattg acattttttt cacctccaat tacatcgagg attttttgta
     3421 ccttatcttt ataactcatg gtattctcct attctattaa tctaaatttt ttgttaagcg
     3481 acgaatatga gccatcaaat aaactaattc actagaagtc agcaaataat tgtactcagt
     3541 ttgtataaac attgccacct gttcaccaca ttcatattct ctaggatatt tatttttcat
     3601 taatgctaac aagtcttcat catcatcgtc acaataagtt gtattttcaa ccaagcgatg
     3661 ggcaaataat tttatatgag ttatgaatct ataataatca atagaatctt ggttaaactc
     3721 agtacaatag aaatcttgaa tgattttctc aattcccata attatttcag tgattttata
     3781 cgactcttga aaattatttt ctaaattagc attaacaaaa tgtagcgcta tgaatgcagc
     3841 ttcgtcaatt ggaagttcaa tacctaactc tttttttatt atttgtaaag cccttttccc
     3901 aagctcaaat tcttctttat aatatttcga gatttcccag cgtaggggat tcgaaataat
     3961 aactccttct ttataacgtt ctatactcga agaaatatga tccgttagat ttatatagat
     4021 aatctcatca aaattattac ctaatgttat tttacctagg ttaattattt tttcggtgat
     4081 gtacaccaac tccaaaggaa cgttaataaa catctctgta aaacgggtca tattatctct
     4141 atttttgaga aaaaaacttt tctcaatatc aattggattt actttatctc cagtttttct
     4201 tccaaaagct attccccttc caaaaagaag aatatcgata tcttttttac tttgcgcaat
     4261 tacggcattg tggtttagta ttcgttttat gatcataaaa tttccctttt aaaataaaag
     4321 gaacctactt aaaaaacatc aagaaacttc ttctgatgtg attaaatagg ttcctgctca
     4381 cttgtattag tgataacatc cttttatttc aatttttaat ggtgacatga agcttaaata
     4441 attaataact ctgtaaccgt ttttatttta tgatatttca atctatttgt caagtactta
     4501 taaaaatttt tttattttgt gtgattcgaa tgtgataatg gcaacagtct cactaaagaa
     4561 atgaaaatca tacatagaaa tttttaaaaa ttttctctat cgtaaacctg ccactagttc
     4621 agaaactata ctagctgatt ccaaacgtaa tatggtcatc tcatctacct tccctaataa
     4681 tggcattgca ccataccaaa caatagtctt attcaccaaa agaataggcg ttactatttt
     4741 tgattgagta aaagacactt taattttctg gctcttcaac tggtctagcc atgctttatt
     4801 tgcaaccgtc tctggtatac atatctcaat ttgactaatc ctaaattctt ttagaaaatt
     4861 catcagtttt gtctggtgca cataaggtaa aataagcaga cattcctgtc tttcccctgc
     4921 taagtcctct ctcaacacct tctcatatct actatcaaca taaacgaatt gtttctcctc
     4981 accctcgatg acacgataat ccatctttcg ataagctact tgtcgtttct gaaacatctt
     5041 ttctaaataa ggaacatgaa tatccacata atcgaaaata cgcaccaaag acttatcctt
     5101 gtagtttcta tgaatccgac ctgcatactg aattaaatta tttttccaag aaaagggtgc
     5161 tgccaagata agcgtgtcca actgaggtaa gtcaaaacct tcgccaatgt attttccagt
     5221 agacaacaaa acaaaccctt tatctaactg ttctaacgtc tccagtaaac tcgttctttc
     5281 tcggactttg gtttttccgc taataatgta acagtcatca acctctttct ctttcaataa
     5341 cttttcaaag acatctatct gttgaattcg attaactaaa accaagatat tccgtccttc
     5401 tgccacttgg gctagaatat ccttgagaat caactgattc ctcgctgagt ctgtagcaat
     5461 ccaatcactg agctgtataa aattacttgc tttggtcttt tcaatctcca aatgaccaaa
     5521 agaagtgaat cttaattgca attgccgttt aaaatccgtt tccctcttat cagcagtatg
     5581 gagtatctca ccaattctct gaaaaacaat aggctcatga ccattcttac gctcaggcgt
     5641 agccgtcaaa ccgtaaagat acttccctct aaactgagca acaacttttt caaacatcaa
     5701 ggcagagaca tgatgacact catccacaat catcatctca tactcatcca aaagactttg
     5761 actattttcc aacttaaata gagattgaat cataacgaca tcaaccagtt tactcagcca
     5821 tttcttagtc ccaccgtact gtccaacata gccgattacc ttttcacgac ctgatgccgt
     5881 ataacggata gcctcctcct cttcgaaagt caaaaagcag tttaagcgat ctagccattg
     5941 gtctaagagt tgcttattat ggactagaat aattgttttt gttttccgtt cagagataag
     6001 agcagcacct aaaacggtct tcccaaaacc agtctccgca tgaagtaaac cattttcttt
     6061 agaagtcata tctgacaggg ctaactcttg ctcaaaagtg agttctccct taaattccac
     6121 gctaatagac ctttgtacct ttctcctatc ttccacagat acctgcttaa atttatcttg
     6181 caatggatat aacaagcctc ttggcagcca taaataataa tcggattctc caaataaata
     6241 cattctctca ggaatttgat aggttggctg tcgcatagcc tgctttaaat aaaattcggg
     6301 attagaaaat gaagccatat ttttcaagaa aaacagtgtc ttgggtgtca cagacgattt
     6361 ttcaagttgg atcatattgg ataagacgat cttcaactcc ttgtccaatt cttgcttgcc
     6421 taactcttct tggattaaca gtgccacttt agcagttgaa accctctgaa tttcttgtag
     6481 atacctccat tggtcttcat aaggctgaaa ctgttcatcc acaaagaccg ttcgcccttg
     6541 atggtaagct tctccttgaa aaggcaaggc aatcaaattt ccaaatcccc cccttaggaa
     6601 ggacatcctg atttggaaac atgcgatcaa aagaatcaaa ggacagttgc atactttcct
     6661 gcattgccag ttctatcagt ttctttccaa acaagcgagc ctctcgactc ggaatcgctt
     6721 cctcaaagaa gaaccaaata tggagtccgt gacccgaacg agaaatctct aaatgggctt
     6781 ccatctggcg ttccctggct attcttcgaa tggttaaacc agcttctttc caatctcctt
     6841 catcaaaatc cagtaccaaa aaatgacagc tatcatctaa gtgcatagga aagataccga
     6901 tagctgtctc cccacaaaaa tgagatttca aaacggagtc cgtcaatgtc tggaaactcc
     6961 gtttttcaag tggcaactgc ttccaaccat aatcatagga aggaaaatac tgaattttcc
     7021 cttgatcatt gataaaactc ttagcataaa catcataacg gccagcaaac acggaggtaa
     7081 acaattgtaa tttttcttgc gtcgtcaagt gactactttg aagttggaac atctcctcga
     7141 accgagagcg ttccatcaca aactgactct tgtctttaag acgtttacac aagaaaaact
     7201 cactatcccc attaaaagaa gaaaagacat caagtacttc taccacaatt ccatccaaag
     7261 acacaaaaat agccataaca atcacctcct tgattcctat aggctgatta taacaagaat
     7321 ggctgaaatt gtacacgaaa ataaaatccg aatagcactc attttgtatg cgactaatag
     7381 tccatctcgc tccggaatgt acgaagtaaa tagagtttaa acgagcagtc tataaaattc
     7441 aaaaaacgca tcata
//
LOCUS       DAVBNO010000076         7385 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVBNO010000076 DAVBNO010000000
VERSION     DAVBNO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.076"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            226..6642
                     /locus_tag="VQ846_001896"
     CDS             226..6642
                     /locus_tag="VQ846_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET3076425.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     VVDKETENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDTKTSNEEARIKE
                     DSNQSQGDNAHSSANKGTENPKKEDRLVYIAEFKDKESGEKAIKGLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKG
                     MISYYLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKE
                     KSSFTIDRNISTIRDFENKDLKKLIKKKYKEEDDFVTGGKRTVELDYKYDDKGNITAY
                     EDESALEYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKFIEI
                     KATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKET
                     KAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLKEDLQREDHS
                     QKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGL
                     KKKNQD"
     gene            complement(6938..7284)
                     /locus_tag="VQ846_001897"
                     /pseudo
     CDS             complement(6938..7284)
                     /locus_tag="VQ846_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2882 a          991 c         1425 g         2087 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttatataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taactacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaagcatct ctgataaaga aaaagtagta
      361 gtagataagg aaacagaaaa taaagagaaa cataaagata ttcataatgc tatagaaact
      421 tcaaaggata ctgaagaaaa gaaaacaaca attattgagg aaaaagaagt tgttagtaaa
      481 aatcctgtga tagacactaa aactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatcccaag gagataatgc acactcgtct gcaaataaag gcacagaaaa tccgaaaaaa
      601 gaagatagac ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagggactat caaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 tcatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccatttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttagggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgagg atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacacggatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccagatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgcg gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctatttgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgacaaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcag gctgatgaaa aggggatgat ttcctattat
     4201 ctagtagatc ctgatcaaga ttcatctaaa tatcaaaagc ttggcgagat tgcagaatct
     4261 aaatttaaaa atttaggaaa tggaaaagag ggtagtctaa aaaaagatac aactggggta
     4321 gaacatcatc atcaagaaaa tgaagagtct attaaagaaa aatctagttt tactattgat
     4381 agaaatattt caacaattag agactttgaa aataaagacc taaagaaact cattaaaaag
     4441 aaatataagg aagaagacga ctttgtaact ggtggaaaaa gaacagtaga acttgattat
     4501 aaatatgacg ataaaggaaa tataactgcc tatgaagatg aaagtgctct agaatatgaa
     4561 actgaaaaat ttgacgaaat aaaatcaaaa atttatggtg ttctaagccc atctaaagat
     4621 ggacactttg aaattcttgg aaagataagc aatgtttcta aaaatgccaa ggtatattat
     4681 ggaaataact ataaatttat agaaatcaaa gcgactaagt atgactcaca ttcaaaaacg
     4741 atgatatttg atttatacgc taatattaat gatattgtag atggattagc ttttgctgga
     4801 gatatgagat tatttgttaa agatgacaat caaataaaag ctgaaactaa aattagaatg
     4861 cctgaaaaaa ataaggaaac taaagcagaa tatccgtatg tatcaagtta tgggaatgta
     4921 atcgaattag gagaaggaga cctttcaaaa aacaaaccag acaatttaac taaaatggaa
     4981 tctggtaaaa tctattctga ttcagaaaaa caacaatatc tgttaaagga taacatcatt
     5041 ctaagaaaag gctatgcact aaaagtgact acctataatc ctggaaaaac ggatatgtta
     5101 gaaggaaatg gagtctatag caaggaagat atagcaaaaa tacaaaaggc caatcctaat
     5161 ctaagagtcc tttcagaaac aacaatttat gctgatagta gaaatgttga agatggaaga
     5221 agtacccaag ctgtattaat gtcggctttg gacggcttta acattataag gtatcaagtg
     5281 tttacattta aaatgaacga taaaggggaa gctatcgata aagacggaaa tcttgtgaca
     5341 gattcttcta aacttgtatt atttggtaag gatgataaag aatacactgg agaggataag
     5401 tccaatgtag aagctataaa agaagatggc tccatgttat ttattgatac caaaccagta
     5461 aacctttcaa tggataagaa ctactttaat ccatctaaat ctaataaaat ttatgtacga
     5521 aatccagaat tttatttaag aggtaagatt tctgataagg gtggttttaa ctgggaattg
     5581 agagttaatg aatcggttgt agataattat ttaatctacg gagatttaca cattgataac
     5641 actagagatt ttaatattaa gctgaatgtt aaagacggtg acatcatgga ctggggaatg
     5701 aaagactata aagcaaacgg atttccagat aaggtaacag atatggatgg aaatgtttat
     5761 cttcaaactg gctatagcga tttgaatgct aaagcagttg gagtccacta tcagttttta
     5821 tatgataatg ttaaacccga agtaaacatt gatcctaagg gaaatactag tatcgaatat
     5881 gctgatggaa aatctgtagt ctttaacatc aatgataaaa gaaataatgg attcgatggt
     5941 gagattcaag aacaacatat ttatgtaaat ggaaaagaat atacatcatt tgatgatatt
     6001 aaacaaataa cagacaagac actaaacatt aagattgttg taaaagattt tgcaagaaat
     6061 acaaccgtaa aagaattcat tttaaataaa gatacgggag aggtaagtga attaaaacct
     6121 catagggtaa ctgtgaccat tcaaaatgga aaagaaatga gttcaacgat agtgtcggaa
     6181 gaagatttta ttttacctgt ttataagggt gaattagaaa aaggatacca atttgatggt
     6241 tgggaaattt ctggtttcga aggtaaaaaa gacgctggct atgttattaa tctatcaaaa
     6301 gataccttta taaaacctgt attcaagaaa atagaggaga aaaaggagga agaaaataaa
     6361 cctacttttg atgtatcgaa aaagaaagat aacccacaag taaaccatag tcaattaaaa
     6421 gaggatttac aaagagaaga tcattcacaa aaatctgatt caactaagga tgttacagct
     6481 acagttcttg ataaaaacaa tatcagtagt aaatcaacta ctaacaatcc taataagttg
     6541 ccaaaaactg gaacagcaag cggagcccag acactattag ctgccggaat aatgtttata
     6601 gtaggaattt ttcttggatt gaagaaaaaa aatcaagatt aagataaaag ctatagaaaa
     6661 aaatggttta tgtactgaga ttagatagtg aggtgatgac atagttttgt gaaaatagcc
     6721 atttataact caattattta gtttacttta ctttactagt gatactattt ggagttatta
     6781 atggacttag tttatataac taatgaattg attgaaaggg ttagtattga caatattggt
     6841 catattgact agaaaataga gtctatcaaa atttaaaggc taatagaggt gatgagacaa
     6901 tttcggctct ttgtcaactg tagtgggttg aagtcagcta agctcgagaa aggacaaatt
     6961 ttgtcctttc ttttttgata ttcagagcga tgaaaatccg ttttttgaag ttttcaaagt
     7021 ttcgaaaacc aaaggcattg cgcttgataa gtttgatgag attattggtc gcttccagtt
     7081 tggcattaga atagtgtagt tgaagggcat tgacaatctt ctctttatct ttgaggaagg
     7141 ttttagagga tgaacttgat tcagattgtc ctcaatgagt ccgaaaaatt tgtcaggctc
     7201 cttattctga aagtgaaaaa gcaagagttg atagagatta tagtggtgtt tcaagtcttc
     7261 tgaatagctc aaaagtttat ctatagtaga ttgaaactag aatagtacac ctctgcttct
     7321 aaaacattgt tagaaatcga tttgactgtc ctgaatgatt tgtcctgtta ttatttcatt
     7381 ttact
//
LOCUS       DAVBNO010000077         7224 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVBNO010000077 DAVBNO010000000
VERSION     DAVBNO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.077"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(83..523)
                     /locus_tag="VQ846_001898"
     CDS             complement(83..523)
                     /locus_tag="VQ846_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET3076426.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLKKETRLRFLEEMSVVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLNGRGPVWWVPFEEMTAETRQAKPDEIRLPAKQNMV"
     gene            complement(525..1562)
                     /locus_tag="VQ846_001899"
     CDS             complement(525..1562)
                     /locus_tag="VQ846_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET3076427.1"
                     /translation="MNQTVEYIKELTAIASPTGLTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLRSNLVD"
     gene            complement(1660..2985)
                     /gene="brnQ"
                     /locus_tag="VQ846_001900"
     CDS             complement(1660..2985)
                     /gene="brnQ"
                     /locus_tag="VQ846_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET3076428.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVIGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(3156..3248)
                     /locus_tag="VQ846_001901"
     CDS             complement(3156..3248)
                     /locus_tag="VQ846_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET3076429.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(3291..3443)
                     /locus_tag="VQ846_001902"
     CDS             complement(3291..3443)
                     /locus_tag="VQ846_001902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET3076430.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLECAHNLPLSQ
                     LADSYD"
     gene            complement(3603..4181)
                     /locus_tag="VQ846_001903"
     CDS             complement(3603..4181)
                     /locus_tag="VQ846_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET3076431.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(4235..5635)
                     /gene="pepV"
                     /locus_tag="VQ846_001904"
     CDS             complement(4235..5635)
                     /gene="pepV"
                     /locus_tag="VQ846_001904"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET3076432.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(5648..6253)
                     /locus_tag="VQ846_001905"
     CDS             complement(5648..6253)
                     /locus_tag="VQ846_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET3076433.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            6424..7224
                     /locus_tag="VQ846_001906"
     CDS             6424..7224
                     /locus_tag="VQ846_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET3076434.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
BASE COUNT         2118 a         1623 c         1444 g         2039 t
ORIGIN      
        1 aaaagtggta cgctgaacac caacaagacg cttaaatcgt gcatcagtta gttgtttact
       61 tgcttcatca ttcatagaac tactatacca tattttgttt cgcaggaagt ctgatctcat
      121 ccggttttgc ttgacgggtt tctgcggtca tttcttcaaa agggacccac cagactggcc
      181 cacgtccatt gagaccgtga ccattcatgt cacctgttcg tctaggaaac agatgccaat
      241 gaagatgggc atcgccattt cctagcagtt cgatattcat tttctcagca gcaaaggcct
      301 tggcaactgc ctcttggacc acactcattt cttctagaaa acggagtctt gtctcctttt
      361 tcaaatggtg caattccgtg acatgttcct tggctagaaa gagactatag cctgcaaaat
      421 actgatggtc tccaatcaca agatagcctg tttccaactc tttgacaaag tagggatttt
      481 ctcccttctt gatgagctca attctctggc aaataaggca catatcaatc tactaaattg
      541 cttctaagat aggcatcgac cattcgttct gttgcgacca ccgagtcaat atgagtgcgc
      601 tcataagaat gactagactc gatacccgca ccaagaaggg catgcttgac ctctgcccct
      661 gcagacatag ccgctgaagc atccgaacca taaaatggat agatgtccag cttaaaagga
      721 atatcttgct ctttagccaa ggcaaccaag tgttgacgga agtcatagtg ataaggacct
      781 gaagcatcct tgacacagat agatactgtg tactcatctg tctgctggtc atctcccata
      841 gctcccatat ccacagccag atattctact acctgagcag gaatgttaga attagcacca
      901 tgtcctactt cttcaaaaac tgaaaaagca aaatgagttg ttactggcaa ttcaatcttc
      961 tctttcttat aaatacgaag caggttgagc aaaatcgctg cgctgacctt atcatccaaa
     1021 tgacgagact tgataaaacc tgtatcagtc acaacagttc gtggatcgaa gctgataaaa
     1081 tcaccgacct caatcccaag agcacgagtt tctttttcat tgcttacttt tacatccaaa
     1141 cgcacttcca tattgtcctg cgtgcgttct gcagttcctg catccttgta gacatggcaa
     1201 gaagtttggt ggatgaggat ggttcctgat accttttgac ctgtgctagc cacatgaacg
     1261 gtacagtttt caccttcaat catgttccaa ggaaaaccac cgatacggtc caatttaaga
     1321 cgaccatctg gtttgacagc acgaacaata gcacccagcg tgtccacatg ggcagtcaca
     1381 tagcgatgtt gctcatcatt ttgacctttg atggtcacat tgacaccacc cttggctgtg
     1441 cgaactggct ggtaaccaaa accttctaga gtcttgacta aataattcga aatttcacga
     1501 gttaagcccg ttggcgatgc gatggctgtc agttctttga tatattctac tgtttgattc
     1561 atttcttcac ctctttcgct acctattata acacatttat catcggataa aaaaagaaaa
     1621 tcactcctgt agacttggct acaaaagtga tttaagtgat tattccattt caaaaacatc
     1681 gctttcttgc ttgtttggta gaaccaatga gagcaagatt ccaacaatgg ctggcaccaa
     1741 ccatgggaga gatgccttgg caaaaggaag aacgctaaca agatttgcaa gaaactcaac
     1801 cttaaacgag cttcctagta cgcttgcaat ggcaataact gtaaccacag caattgtcaa
     1861 ctgcatacct ggttttgaaa gggcaacaaa tttgttgaca atgacaatca taacgatagc
     1921 aatcgtgatt gggtacaaga taaccagtac tggaattgag tacttgataa tcgcatcaag
     1981 acccaaattg gcaatagcaa atccaatcaa ggtaaaggct gtcgcataaa ccttgtagct
     2041 gatttgtggg aagcgctcat taaagaactc agctgttgac acaatcaaac caacagtcgt
     2101 tgtgaagcag gttacggtaa ccatagctgc aaggaagagt tgagctgttg agccaaagat
     2161 ttcttgagtg gcttgtgaca agatgtaaac acctggtgtt ccacccttca tcgcttcagc
     2221 tggtactggg aaatgatttc caagaaaacc taaaccaatg taaagagcgc tgaaggcaag
     2281 ggcaacaacg ataccaataa cccaaatagt tgaaatgtat tctttcttac ttgaaaatcc
     2341 aagttgtttc aaggtttgaa ctgcgattac gctaaaggcc actgaggcaa gggcgtccaa
     2401 ggtattgtaa ccttctagga aacctgtacc aaaggcagaa gcttgataag cagctgaagc
     2461 agcttgagga cttgttccac catatttgat agctcccaga acgaccaaga taacaatcaa
     2521 aattgcaaag actggcgtta aaatacgtcc aatgcggtct aagatttttg atggattaag
     2581 cgaaatcaaa taggctgccg caaaatacag aaccgtaaat acaatcaagc caagtccttt
     2641 atttgcatcc gacaaaaggg ggctaatccc tacttcgtaa gctgttgtag cagtacgtgg
     2701 ggtagcaaag aatggaccga ttgacaagta aagaactgag aggtaaagag tcgcaaacca
     2761 aggcgctatc ttcgttgaaa tctcgtagat atatccttta ggatttagcg ttccaataat
     2821 aagggtcaag acggcgatac caacgcctga aaagacaaaa cctgcgatgg caggaagaaa
     2881 atgttctcca gatagagcac ctagagaagg cggaaaaatc aagttccccg caccaaaaaa
     2941 tattccaaac aggagcaaac ctgttagggt acctttttta gccataaaaa tctccttcat
     3001 atttataaaa tactgaccta gtataccacg attaggagtc tgaaaaaagc atcacgaggc
     3061 aaatattaaa tgttttcaat tttcttaaaa ataagaatta tctaaaaata aaactctcct
     3121 acccagtcca caccaagtag gagaaatgca aaattttaaa gttcttcaaa ggctaacatg
     3181 ccaccctgga cattgataac attataacct tgttctaaca ggaattggca agcacgcgcc
     3241 gatctcattc cagatttgca aataatataa tgcaagcgat ctttgtccaa ttaatcataa
     3301 ctatcagcca attgactaag cggtaggttg tgggcacatt ctaaatgaag agctgcaaac
     3361 tcatccactt ctctcacatc cactagagaa agctggtcgt tttggtaaag ctggtaaaat
     3421 gcgtcaaagg ctatttcttt cattcttttt cttctaatac tcttcgaaaa tctcttcaaa
     3481 ccacgtcagc gtcgccttac cgtgcgtatg ttaccgactt cgtcagtttt atctgcaacc
     3541 tcaaagctgt actttgagca gcctacgact agcttcctag tttgctcttt gattttcatt
     3601 gactataaaa tggttttaat tctttttttc aaatctggca ctacttctgc ctcaaaccaa
     3661 ggatttttgg ccatccagat ttgatttcgt ggtgatgggt gaactagcgg aaaataggct
     3721 ggcagatagt ctttatagtg tttcaccctc tccgttacct tcccactgat tttctcctgt
     3781 aaatagtagg cttgggcata ttgcccaatc aagagggtta actgaatatc aggcaattcc
     3841 tgtaagagct gcggatgcca tttttctgca aaacctgtac gaggcggaag atcacccgac
     3901 ttgccatgtc ctggaaagta gaaatccata ggcaaaacag caaaataacc tgaattgtaa
     3961 aaggtatctt catccacacc tagccagtcc cgcaagcggt caccactttt atctttccag
     4021 taaaggcctg cttcttgagt tttaagtccc ggagcctgac cgatgatatt gatgcgagca
     4081 gtttttggcg ctgcaaagag aggctcaatg ccacgctctg tatagctggc attctgtgaa
     4141 tccgccatga tagcctgttt gattctttcg atttgagaca tctactttcc ctccaaaaag
     4201 aagtccaagc ataagatctc agacttctat cgttttattt gatcaattcg taaatagctt
     4261 cggcataaat tgctgctgct cggaaaagat catccaaggc gataaattca ttggcttggt
     4321 gcatggtatc aatcgagtct gggaacatag caccgtaggc aactccgcgt tctagcaagc
     4381 gaccaaaggt tccaccaccg atgacttgtt catgaccttt aaggccagtt tgtttttcat
     4441 agatattcaa caaggtttgc acaagtggat cttccattgg cacatagtga ggcgtgtgac
     4501 cgtgttcaga caggctaaca gaaacaactg gcaagttttc aaggattgac ttgatttgtt
     4561 ctggacttgt tccttttgga tagcggatgt tgagggcaat ggtattatca gcacttgttt
     4621 catcgaagcg gaagacgccg gcattcatag aaagagcacc catcttttca tccacatgag
     4681 caatcttaag attttcaccc tcatgatcgt tcaagagaat tttacctgcg atgtcaaggt
     4741 agtctttggc tggaccagca aagccaaact ggctgaggaa gagggcaagg taagttgcgc
     4801 cattgacacc tgaagcaggc atagcaccgt gggctgattt accaatgatc gtcaccttgt
     4861 atttgccagc ttcttcttgg agttctcctc taagtttgtg ttctgcaaca aaggcatcta
     4921 gtttagcttg caagtcagcc aaatcacctg aaacgactgc tgttgctgat tctggtacca
     4981 tattttcacg taaaccacct gtaaagctgt gaagacgggc agcacctgta ttttctcctg
     5041 caaagtggag gtattccgtg atatttcctt tttcaccatt gatgattgga aattcagcat
     5101 ctggagagaa accgaaatct ggtttggcaa gtcctacgtg ctcaaagtag tagtccatgt
     5161 ctgcccagcc tgattcttcg tctgttccaa cgatgaagcg aactttctta gaagttggaa
     5221 gacccaattc tttgatgatt ttcaaaccat agtaacaagc tgttgtagga cccttatcgt
     5281 ccgaagcccc gcgcgcataa aggcgaccat ctttgatagt tggtgtgtaa ggatctgtgt
     5341 cccaaccgct accagcaggc accacatcca tatgggcaaa gattccgaga acttcttctc
     5401 catcaccaaa ctcaaaatgt cctgcgtagt tatcgacatt cttagttggg tagccatcgc
     5461 ggtctgcgat ttcaaggaat ttctccaagg cttttactgg accaggtcca aatggatgct
     5521 gggcatcagc cttgctatca tcacgttctg agttgatttc caaaaggcta aacaagtcag
     5581 ccaagaggtc ttctttgcgt ttttctactt ctgctgtaaa atcaattgct gtcatttttt
     5641 cttctttcta tcttttctcg atgatttcat ctactggcaa gcggtagctt ggttccaatt
     5701 tgtcgtctgt gtaacccact gtgatcaaga gttctgggcg gaaacggtct tcgatttcca
     5761 aaacttcatt aacttttgat ttgtcaaaac caaggataat attggaacca attccttggt
     5821 ctgtaagagc gagaaccaag ttcatagcaa ccaaacctgc attgagtgct aggtagtcgc
     5881 tgacttgttg ttcattgtag cgtgcaaatt cagctggcaa atttttcatg aagtattgaa
     5941 gttgctcttc agaaaagtta ttagcaccac caacacgggc aatcttacga gcacgtttgg
     6001 ctaagtccgt atctgtaaac aaggcaatgg ttacaggcgc tgatgatacc tgttcaaaat
     6061 tggaaccata agctaacttt gccagttcag catttttctc acgtaccacc acaaatttcc
     6121 aaggctggct gttgtgggcg cttggcgcca aggttgcaat ttcgatagcc gtacgcacat
     6181 cttttggatc aacaggctta tcagtaaaat gcttagtcgc atgacgtttt ttatttaatt
     6241 caagaaattt cataatcgat ttccttttct aattctactg cttccattct accataattt
     6301 gaaagagctt gcagggattt ttcatgatta agtttttcta tcacagacat aaaagatata
     6361 tattggttta tcaggaacat ttctgataaa ctagcaagta ctttacattt gaatggagac
     6421 agtatgaaaa aatttagcct attactagct atcctaccat ttttggttgc ctgtgggaat
     6481 caagctacac ccaaagagac tagcgctcaa aagacaatcg tccttgctac agctggcgac
     6541 gtgccaccgt ttgactacga agacaagggc aatctgacag gcttcgatat cgaagtttta
     6601 aaggcagtag atgaaaaact cagcgactac gagattcaat tccaaagaac cgcctgggag
     6661 agcatcttcc caggacttga ttctggtcac tatcaggctg cggccaataa cttgagttac
     6721 acaaaagagc gtgctgaaaa atacctttac tcgcttccaa tttccaacaa tcccctcgtc
     6781 cttgtcagca acaagaaaaa tcctttgact tctcttgacc agatcgctgg taaaacaaca
     6841 caagaggata ccggaacttc taacgctcaa ttcatcaata actggaatca gaaacacact
     6901 gataatcccg ctacaattaa tttttctggt gaggatattg gtaaacgaat cctagacctt
     6961 gctaacggag agtttgattt cctagttttt gacaaggtat ccgttcaaaa gattatcaag
     7021 gaccgtggtt tagacctctc agtcgttgat ttaccttctg cagatagccc cagcaattat
     7081 atcattttct caagcgacca aaaagagttt aaagagcaat ttgataaagc gctcaaagaa
     7141 ctctatcaag acggaaccct tgaaaaactc agcaatacct atctaggtgg ttcttacctc
     7201 ccagatcaat ctcagttaca ataa
//
LOCUS       DAVBNO010000078         6944 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVBNO010000078 DAVBNO010000000
VERSION     DAVBNO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.078"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(57..287)
                     /locus_tag="VQ846_001907"
     CDS             complement(57..287)
                     /locus_tag="VQ846_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076435.1"
                     /translation="MKRVILVAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(305..946)
                     /locus_tag="VQ846_001908"
     CDS             complement(305..946)
                     /locus_tag="VQ846_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET3076436.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(958..1692)
                     /locus_tag="VQ846_001909"
     CDS             complement(958..1692)
                     /locus_tag="VQ846_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET3076437.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(1695..1892)
                     /locus_tag="VQ846_001910"
     CDS             complement(1695..1892)
                     /locus_tag="VQ846_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076438.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            complement(1892..2098)
                     /locus_tag="VQ846_001911"
     CDS             complement(1892..2098)
                     /locus_tag="VQ846_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET3076439.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(2108..2422)
                     /locus_tag="VQ846_001912"
     CDS             complement(2108..2422)
                     /locus_tag="VQ846_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076440.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(2647..2727)
                     /locus_tag="VQ846_001913"
                     /pseudo
     CDS             complement(2647..2727)
                     /locus_tag="VQ846_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(2718..4007)
                     /locus_tag="VQ846_001914"
     CDS             complement(2718..4007)
                     /locus_tag="VQ846_001914"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET3076441.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRAYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVSQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     RQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(4104..5333)
                     /locus_tag="VQ846_001915"
     CDS             complement(4104..5333)
                     /locus_tag="VQ846_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET3076442.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SILCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAVIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFFDNS"
     gene            complement(5526..6377)
                     /gene="rgg"
                     /locus_tag="VQ846_001916"
     CDS             complement(5526..6377)
                     /gene="rgg"
                     /locus_tag="VQ846_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HET3076443.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            6501..6842
                     /locus_tag="VQ846_001917"
     CDS             6501..6842
                     /locus_tag="VQ846_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET3076444.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
BASE COUNT         2249 a         1442 c         1278 g         1975 t
ORIGIN      
        1 ctctttgatt ttcattgagt ataatacaaa aatccgatga acttcaccgg actcttttat
       61 tttgaatttt tgcctgcttt acgcttttca gcgatttcgg ctgcctttcg aggcaagaca
      121 atttccgtta tgtaagccgt cccaaaacgc agtacacctg caataggagc aaagacaact
      181 gctagatagt tatagaagaa atcgcctttg aaggcataag ctagcgctcc aatgatgaaa
      241 aatagaacga ctgcctgaat cactgctact aaaattactc gtttcatgtg acctcctgac
      301 tctattatag catgagaatc atcaaaaagc cgactaaatt attcaaagcg tgaagagaaa
      361 tactgtagac cagacctttt ctgctaatgt aagccaaacc caaactaaaa ccaaggctaa
      421 aatagacaaa aaattgttgc acatcacctg gaaaatgaat caaggcaaat agaagactag
      481 ataccagaag aaaaatcagg gttcgtttac tattgtcctg cttaggaaag agatagcgtg
      541 ctaacatccc tctaaaaaca atctcttccg tcaaaggagc aaaaataacc acagcaaaga
      601 atgagaaaag tggttgagac aaggtcaagt ctgtcgctat ttgttgattt actgaaggat
      661 catctggcaa gaagaattga acgaccagag ataagaacca aaccaagaca ggaagccaaa
      721 taaatcgatt aaagccgctc ttctcaatat gaacaggagc cttctgatac catttgtaaa
      781 tgccgtacac atatactcca gccaaggcca catagagtag agtaacagca tagggtgaag
      841 cgcctaaagc aagcgacgca gtcgcgagcc cctgaataaa gccatagata aataaaaagg
      901 atagaagggc tagaagaatc cagccaaggt ttttaagtaa tttcatagat aactccttta
      961 tttgaaataa cgttttacca taggtaactg catcacattg atataaacat ggatggctcc
     1021 tacaagcaag aaagctagta actgaatctc tcctgtcaag aaagaaatga taataagaaa
     1081 aatatataag gctggtaaga catattggtg taattggaat aaaattcgaa aactctgttc
     1141 caaattagcc tgacgctccc cttcatcata agaatttata tagttcaaga catcctttgg
     1201 tgtagcgaaa aattccaaat caaactgacg aacaatcgca atggttttaa aaagagattt
     1261 ttgagcgact aagaatacca caaagagtaa gaaagaaagg aaaaatgttt gagggtttgt
     1321 atgcaatata atcacctcac ttaatgaaat aaaaatagcc aatggaatcg ctacacctgt
     1381 aatattaaaa gcaatggttc caaactcaag attccgatac atttgcacat aataggtttc
     1441 attcagatcg tcatccattt cctcttgata caaggaatga aattttctgc ttttctttaa
     1501 gaaattgaaa gtcaaaaaca tactaatgaa acctatcagt aaacaaatag ctgatatcca
     1561 tggcatcaag gcttttacat ctaaaataat ttcgtgggat tcgacacgtg ccttaaacat
     1621 ccctacaaac atgcccaaga acccccccaa gacaatagac atcaaaaata acaatctacg
     1681 tttctttttc atattcattc tcctttttca cttgctagat ttttggattt cttttcaatc
     1741 cattcagtta ctgggatgag agcaaagtag acccaaacaa attggtcgct ttgataggga
     1801 ttaaaccagc ttaggtccat cccaatcagt agaaatacgc tgactaataa agctatgacc
     1861 actacataat aaatcacttt atacttgttc atcactcgtc ctcctccaaa cgaaataccg
     1921 attcgactgt ttcgttgaaa atttgagata ttttcagggc aatgataatg gatggggtgt
     1981 actcatcccg ttctagtagg ctaatggtct gtctggaaac ccctgccagt ttggctaggt
     2041 cggtttgatt gagaccatcg cgagctcgaa gctcttttag acgatttttt agttgcacgt
     2101 tacacaccta ctctccgtca aattcaacgg tttggatatc ttcaatacgt tgcaacttga
     2161 atttttcttt tcccgtatta tcaacactac gtagtttgac ccattcctca tcaacatcca
     2221 caacttccca gttatctggc ccaatataca ctcccgttat aattggttcc tttccaatca
     2281 tttcttgtaa taatctcgac atttctgcgt ttcctttctc ttttcgctca agttttttga
     2341 ttttattctc tagtttcttg atttttttag aattattaga ataaaagaaa atcataaata
     2401 gtataaatcc tagtacccac attataactc ctttctgctt cctatttctt aacttgaatt
     2461 cattgtaaca tatctttttc tttttgacaa ttatagttgt caaaaagttt atgatttttg
     2521 tcattttgca aaagaaaaag gtcaggagta agttcctgac cactttatct atcattaata
     2581 ctcttctaaa atctcttcaa accacgtcag cttcaccttg ccgtaggcat ggttactgac
     2641 ttcgtcagtt tcatctacaa cctcaaaacc atgttttgag caacctgcgg ctagcttcct
     2701 agtttgctct ttgattttca ttgagtataa aatcctagtt tttcaaagat ttctgagaag
     2761 ttttggctga ttgtctcaag tgacacttgc acttcttctc gggtttggtt gttcttgact
     2821 gtcacttgtc tgctttcgac ttcgctctct cctagggtga tgagggtctt agccgcaaag
     2881 acatcggctg acttgaactg agctttgagc ttacggttga ggtaatcacg ctctgctttg
     2941 aaaccttgtt ggcgaagagc ttggactagt tctagggctt tgacatttgc tccatcaccc
     3001 aagactgcga tatagacatc tagggcattt tcgataggga gggccacgcc ttgtttttca
     3061 aggatgagaa gcaggcgctc tacaccaagc ccaaaaccaa atccagcagt ttcagggcct
     3121 ccaaagtaag caaccaaacc atcgtagcga ccacccgcac agactgtcaa gtcattgccc
     3181 tcaatctctg tgataaactc gaaaatggtg tggttgtagt agtccagacc acgcaccata
     3241 ttggtatcga tgatgtaatc tactccaaga ttttccaaca tctgactcac agcatcaaaa
     3301 tgagtttggc tttcttcatc aagaaagtcc aagatagacg gcgcattctc tactgccacc
     3361 ttgtcttctt tttccttaga gtccaagaca cgaagaggat tttcctccaa gcgacgttgg
     3421 ctatccttag acaaggtctc cttgagcggt gtcaaatagt caatcaaggc ttggcggtag
     3481 gctgcacggc tctcaggatt tccaagagtg ttgaggtgca atttgacacc ttgaataccg
     3541 atttccttca aaaaatgggc tgccataacg attgtttcca catcggtagc tggattgcta
     3601 gaaccaaaac actcaacacc aatctggtgg aattggcgca agcgccctgc ctgtggacgc
     3661 tcataacgga acataggtcc catgtagtag aacttgcttg gcttttgcac ttctggagcg
     3721 aagagtttat tttccacata ggcacggaca acgggcgcag ttccttctgg acggagggta
     3781 atatggcggt cacccttgtc atagaaatcg tacatttcct tggttacaat atctgttgta
     3841 tctccgacag agcggctgat aacctcgtaa tgctcgaaaa taggcgtacg cacttctgcg
     3901 tagttgtagc gcttgaaaat ttcacgggca aagccctcaa cgtactgcca cttagcagac
     3961 tcagcaggta aaatatcctg cgttcctttt ggtttttgta atttcatagg gaatcctctt
     4021 taaacttaat agtcttattt taccataaat agagggatta aaacagtaag aaaaaaatta
     4081 ggatttagat atcatttttg agattaagaa ttgtcaaaaa aaatagctag caaggaaaga
     4141 ccaacaaata gcatccaagt caactgtata ttccatacgg ctactagtga aaaacaagct
     4201 gttcccacag gtatggataa ggtaaacaat agacctaaaa aattactagt acgagctaga
     4261 acctctggag ctagattttt catgagcatg gcactaatct ttggttgaac tttaccagac
     4321 acatacagag taaagaagag aaatagcaaa ccaagcacga cttgattgaa taaattagcc
     4381 aaaccaacta gactaagccc tacggtcgcc cacatcatca atctaggcaa ggactgcttc
     4441 ccaaaataat cattgcccgt aaggctactg atgatgactg ctactaaaac acagaattga
     4501 ttgataaata gtgcctctgt ataagaaaaa ttcaagagag aatggctcaa aaagaagata
     4561 ttataaattc cacccaaagc gccacccaag gaattaataa gcaagacagc aaagagcata
     4621 aaaccaaagt ttttctgtcc acttttaaga aaaacgagac gtaaatttcg gtaaattgtt
     4681 aggaactggt ctttgataga aagcttctca ttttttaagt tttcaccatc agcagatgac
     4741 attgacaggc tcaatttgct ttttcctaaa aagaggatag tggctgatac taggaaaaag
     4801 caggcattga ttcccgcaac gagagaaaaa ttgttgaccg atagagctaa gagccagact
     4861 ccgaaagctt gaccaccaat agctgaaata taggtgatga actgtgaaaa agaataagcc
     4921 tccatcagat catcttcagc tactttttcc ttaataagag gcatacgcag gccacctgca
     4981 aaatcactga tgatatcact aatgacattg atcaaacaca ggatagaaaa ggcaaagaga
     5041 ctagcttgct gaacaactag ggctgctaga aaaaatagaa ccgcctgaaa caaaccgcta
     5101 tagaccatcc atttgacctt gtccctcgtg taatctgccc gaatccctgc aaaaactgta
     5161 aagagggtcg gaagaatcat gacaatattc gccatagcaa cagcaaaaga tgcttgtgac
     5221 aaggtcgatg catagacgat aaagaccagg ttgaaaatcg aagcaccaaa agcattgaag
     5281 aagcgtgata aagtcaaaat tctataagct ggatttctaa acaatagttt catagataat
     5341 ctcccttctt gttcgaacct ctatatgaag tatgactata gtatagcact tagaaatttg
     5401 agagggagaa ctcttgaata tatgcaacaa ataaaatgag caaaaagaaa cgatagaact
     5461 tttgtaatga cctgagagag ggcaatagtt ctatcgcttc aaattttttc ttggtttgca
     5521 gatattcaac attcggtcgt atttctatag tattcggcag atttattaca gccaagcatc
     5581 tcaaaaatac ggacagcatc ctccatcttt ttctggcctt ccttgactct accttgcttg
     5641 ctatcaagga gaccttctgc ccacagataa acaattcgga aataggtctc attttccttg
     5701 tagaaatgct cttcgataac acgtttaaaa taataggcat tggtaaattc ttcacactca
     5761 atactagcta aaaagccgtt caatagtata gtatgaaaaa ggtttcgatt gccagacatt
     5821 tccattagaa aatcagattt acgtaccatt tctcgtacat atctagtaaa aagagaaaca
     5881 gataaaaatg gagaactgac tgaaaataaa ttgagttcat agattcccca gatctcggta
     5941 gaaaacaaat aatcatgaag gacttttcct tcctctgctg ttaagtctac cctttcatct
     6001 atgctcttca tataagactt gataataatg gcatttagaa tatgtttctg tttgttgtga
     6061 gaatgggcat gcttttgata ctccctgcga tataagtcct caagaggtgc tatattcttt
     6121 ggttccaaga catctgtaat ttcttttctc aactcagaat ctgtatcata ctggaaacct
     6181 ctcgccagaa agaggatctc ctccacactg gcagatatat tttccagagc aaatagaaac
     6241 ttttccaccg aaagctcact ctgacctgtt tcaaaacggg acaacataga cggcgaaaat
     6301 tgtcctccgg ttgcttgtct cagtgagata tttcttgact ctcgtaattg tctaaagact
     6361 tttccaatct gctccataga cttccccttg attccgtatt ttcttcattt tatcatattt
     6421 ttcagaaaat taatcaaaag cttgccaaat tgtcagaatt atgagaaaat agaggatatt
     6481 tatcacatgg agggactgct atgagagacg atatcaaaat caatgaccgt gctttggcct
     6541 tgcaagacca aattatcgaa aaactagaga aagtttttga tacagatgtg gaattggatg
     6601 tttacaatct aggtctgatt tatgaaatca atctggatga aacggggctc tgcaagattg
     6661 tcatgacctt caccgatact gcctgtgatt gcgccgaaag cctgcctatt gaaattgtgg
     6721 caggtctgaa acaaatcgag ggtatcaaag atatcaaggt tgaagttacc tggtcgcctg
     6781 cttggaaaat cacacgaatc agtcgctatg gccgtattgc ccttggacta ccacctcgtt
     6841 aagcaggcca atcactttta aagatgaaaa aagcaagccg aaaatggctt gctttttgat
     6901 actcaatgaa aatcaaaggg caaactagaa aactagccgc aggt
//
LOCUS       DAVBNO010000079         6792 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVBNO010000079 DAVBNO010000000
VERSION     DAVBNO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.079"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..379
                     /locus_tag="VQ846_001918"
     CDS             <1..379
                     /locus_tag="VQ846_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630-like element IS630-Spn1 family
                     transposase"
                     /protein_id="HET3076445.1"
                     /translation="RGKVSGRRYQRISLVAGLTNGELIAPMTYEETMTSDFFEAWFQN
                     FLLPTLTTPSVIIMDNVRFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKK
                     HLKKVLPSCNTFYEAFLSCSCFN"
     gene            440..1402
                     /locus_tag="VQ846_001919"
     CDS             440..1402
                     /locus_tag="VQ846_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET3076446.1"
                     /translation="MNMSNITIDGQEIDLSQTMPSYAQVVSLYDDTSISVSSNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVEPLRYE"
     gene            1579..2586
                     /gene="gap"
                     /locus_tag="VQ846_001920"
     CDS             1579..2586
                     /gene="gap"
                     /locus_tag="VQ846_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET3076447.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2653..3489)
                     /locus_tag="VQ846_001921"
     CDS             complement(2653..3489)
                     /locus_tag="VQ846_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET3076448.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTFTTLSNTFEKELKK
                     NG"
     gene            3615..5810
                     /locus_tag="VQ846_001922"
     CDS             3615..5810
                     /locus_tag="VQ846_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET3076449.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVDLTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIANHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5863..6015
                     /gene="rpmG"
                     /locus_tag="VQ846_001923"
     CDS             5863..6015
                     /gene="rpmG"
                     /locus_tag="VQ846_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET3076450.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6025..6201
                     /gene="secE"
                     /locus_tag="VQ846_001924"
     CDS             6025..6201
                     /gene="secE"
                     /locus_tag="VQ846_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET3076451.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6256..6792
                     /gene="nusG"
                     /locus_tag="VQ846_001925"
     CDS             6256..6792
                     /gene="nusG"
                     /locus_tag="VQ846_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET3076452.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2114 a         1190 c         1465 g         2023 t
ORIGIN      
        1 aagaggcaaa gtatctggaa gaagatatca gaggatttct ttggttgcag gtctaacaaa
       61 tggtgaatta atcgctccaa tgacttacga agagacgatg acgagcgact tttttgaagc
      121 ttggtttcag aattttctct taccaacatt aaccacacca tcggttatta ttatggataa
      181 tgtaagattc catagaatgg ggaagctaga acttttatgc gaagagtttg ggcataaact
      241 gttacctctt cctccctact cacctgagta caatcctatt gagaaaacat gggctcatat
      301 caaaaagcac ctcaaaaagg tattaccaag ttgcaatacc ttttacgagg cttttttatc
      361 ctgctcttgt ttcaattgac tatatatcac ggatacagtt gggaaagaag ctattaaata
      421 tttggagagt ccttttggga tgaacatgtc aaatattact atagatgggc aagaaataga
      481 tctgagtcaa acgatgcctt cttatgctca agtagtgagt ctttatgatg atacaagtat
      541 ctctgttagt agtaatgaga cggacaaagt gttggctgga tccctctata ctgatacaaa
      601 tgaacaggga ttaacgattc caagcagttt actaaaaaat tggaatgaac agacaggaaa
      661 aaatttgaca gctaatgatc ttattggcaa atcagtctca gccagcattg tagaaagtgc
      721 tgccgaaact agtaagattg ctcaatttca aacgaagatt gtacgtgtaa tcaatgatga
      781 agatgacatg gaggacagca acagtttcat gctgtctcat caaatggaaa cgattttgaa
      841 agaggctgga tttacgaaag ctgtatctta ttttatcttg gaactcaaag atccatcaca
      901 gacaaaagta gtaacagaag aattacagaa aaataagaag tatactgtgc tttctcaaca
      961 gagagttctt gatattgtga ttacctttat tcgtgttatt cagggattat tgattgtgct
     1021 ttcatcacaa gctattgtgg tagcagcggt tatgattggt atcattattt acatcaatat
     1081 catgcaacgt tctaaggaaa taggtgtcat gaaggcagtt ggttatcaga atcgtgatgt
     1141 caaaggaatt tttatttacg aggctatctg gattgtaggc atcgccttgc tgctggcatt
     1201 tttggttgca caaggggtgg gaagtttggc gaatgcgatt gtaagtcact tttacccatc
     1261 catcactaag gtttttgaat taaatctttt atctgtttta ggaactctag ttttcgctct
     1321 attacttggt tatgtctcag cctacttccc ggcgcgtaag attagtaaaa tggatcctgt
     1381 agaaccgtta cgctatgaat agtaagggat tccttgattt acagtcttaa aaagaaacta
     1441 acatagaaaa acaatggcca tacttgcaat tcaactcaaa tagttgtata ataggtttgt
     1501 tgaaaggtga tttgtagtga ttcaagttac tcttttcaca ataaaaattt catgattttc
     1561 ataaggagga aatcactaat ggtagttaaa gttggtatta acggtttcgg acgtatcggt
     1621 cgtcttgctt tccgtcgtat ccaaaacgta gaaggtgttg aagttacacg catcaacgac
     1681 cttacagatc cagttatgct tgcacacttg ttgaaatacg acacaactca aggtcgtttc
     1741 gacggtactg ttgaagttaa agaaggtgga tttgaagtta acggtaaatt cgttaaagtt
     1801 tctgctgaac gtgatccaga gcaaatcgac tgggctactg acggtgtaga aatcgttctt
     1861 gaagctactg gtttctttgc taagaaagaa gcagctgaaa aacaccttaa aggtggagct
     1921 aaaaaagttg ttatcactgc tcctggtgga aacgacgtta aaacagttgt attcaacact
     1981 aaccacgacg ttcttgacgg tactgaaaca gttatctcag gtgcttcatg tactacaaac
     2041 tgcttggctc caatggctaa agctcttcaa gataactttg gtgttgttga aggattgatg
     2101 actactatcc acgcttacac tggtgaccaa atgatccttg acggaccaca ccgtggtggt
     2161 gaccttcgcc gtgctcgcgc tggtgctgca aacatcgttc ctaactcaac tggtgctgct
     2221 aaagctatcg gtcttgtaat cccagaattg aacggtaaac ttgacggatc tgcacaacgc
     2281 gttccaactc caactggatc agttactgaa ttggtagcag ttcttgaaaa gaacgttact
     2341 gttgatgaag tgaacgcagc tatgaaagca gcttcaaacg aatcatacgg ttacacagaa
     2401 gatccaatcg tatcttcaga tatcgtaggt atgtcttacg gttcattgtt tgacgcaact
     2461 caaactaaag ttcttgacgt tgacggtaaa caattggtta aagttgtatc atggtacgac
     2521 aacgaaatgt catacactgc acaacttgtt cgtactcttg aatacttcgc aaaaattgct
     2581 aaataattct tgagttgata gaaagcaagg ctttgtggtc ttgcttttta tatagaaaaa
     2641 tggatgacac gatcatccat tcttttttaa ttctttttca aatgtatttg aaagggtagt
     2701 gaaagttagc ctctctaaag taagtgggtg ggtaaaggaa agtcggaagg catgaagcat
     2761 aagccggctt gtctttgatt tactattata gagagggtct cccaggatag gaagattatg
     2821 atgcaaaagg tgcacacgaa tctgatgggt tcgccctgtc tttagcttgc aatgagccaa
     2881 ggaagtcttg tttgagaatt gctttaatct gcttacatgc gtttcagcat attgcccatt
     2941 ttttgcatca actattcttt ttctacgatc atggcgatca cgtccaattt tgtctctgaa
     3001 aacaagttct tttctgttga tatttccatc aactagagcc caatattctc tagaaatctc
     3061 ttttttctcc aataagcgat tgagaatggg caggataaaa ggatttttgg caaagagaac
     3121 taagccactg gtttccatgt ccagacgatg aacgacatag caggtttggc caacataggt
     3181 actgacatgg ttaagaaggg caatttcgtt tggttgatta ccatgcgttt tcatcccctc
     3241 tggtttgttt acaataatca agtgttgatc ttgataaact tcctgcacta agtctgggtt
     3301 gccccaaggg atcgtctttt ggggataatc ttcctcgtca aaagtcaact ggcaaacatc
     3361 tccaggattt acgatttcgt tccagtggac ttcttcttga tttatcaaaa tatgtttctt
     3421 gattctcaaa aaatgacgga tttttctagg gatgaggagt tgttcctcaa gtaattgctt
     3481 taccgtcatt tgaggtatag aggcgggtaa tgtaaatgtg aattgcatac agatattgta
     3541 acaaaaaaag ccctatttgg ataggaaata gctaaattct tgtcttccta tgatgaagat
     3601 gataaaataa acgcatgaaa ttagataaat tatttgagaa atttctttct ctttttaaaa
     3661 aagaaacaag tgaactagag gactctgatt ctactatctt acgtcgctct cgtagtgatc
     3721 gaaaaaaatt agcccaagta ggtccgattc gaaaattctg gcgtcgttat catctaacaa
     3781 agattatcct tatactaggt ttgagtgcag gcttgctagt tggaatctat ttgtttgctg
     3841 tagccaagtc gaccaatgtc aatgatttgc aaaatgcctt gaaaactcgg actcttattt
     3901 ttgaccgtga agaaaaagag gctggtgcct tgtctggtca aaagggaacc tatgttgatc
     3961 tgactgatat cagtaaaaac ttgcagaatg ctgttattgc gacagaagac cgttctttct
     4021 ataaaaatga cgggattaac tatggccgtt tcttcttggc tattgtcact gctggacgtt
     4081 caggtggtgg ctctaccatt acccaacagc tggctaaaaa cgcctattta tcacaggatc
     4141 aaactgttga gagaaaagcg aaagaatttt tccttgcctt agaattaagc aaaaaatata
     4201 gtaaggagca aattctaacc atgtacctta acaacgctta ttttggaaat ggtgtgtggg
     4261 gtgtagaaga tgcgagtaag aaatactttg gagtttctgc atcagaagtg agtctggatc
     4321 aagctgcgac tctggcaggg atgctcaagg ggccggaact gtataatccc ttgaattccg
     4381 tagaagattc tactaatcgg cgcgatactg tcttgcagaa tatggttgca gcaggatata
     4441 ttgataaaaa ccaagaaacc gaagctgctg aagttgatat gacttcgcaa ttgcacgata
     4501 agtatgaagg aaaaatctca gattaccgtt acccctctta ttttgatgcg gtggttaatg
     4561 aagctgtttc caagtataat ctaacagagg aagagattgt caataatggc taccgcattt
     4621 acacagagct ggaccaaaac taccaagcaa atatgcagat tgtttatgaa aacacatcgc
     4681 tatttccgag ggcagaggat ggaacgtttg ctcaatcagg aagtgtagct ctcgaaccga
     4741 aaacaggggg agttcgtgga gttgtcggtc aagttgctga caatgataaa actggattcc
     4801 ggaatttcaa ctatgcaacc caatcaaagc gtagtcctgg ttctacaatt aagcctttag
     4861 ttgtttatac gccagcagtt gaagcaggct gggctttgaa taagcagttg gataaccata
     4921 ccatgcagta tgacagctat aaggttgata actatgcagg gatcaaaacg agtcgagaag
     4981 ttcctatgta tcaagccttg gcagaatcgc ttaatctacc tgctgttgcc actgttaatg
     5041 atttgggtgt cgacaaggct tttgaggcag gcgaaaaatt cggactcaac atggaaaagg
     5101 tcgaccgtgt tcttggtgtc gccttgggaa gcggtgttga aaccaaccct cttcaaatgg
     5161 ctcaagcata cgctgccttt gcaaatgaag gtttaatgcc tgaagctcat tttattagta
     5221 gaattgaaaa tgctagtgga caagttattg cgaatcataa aaattcacaa aaacgggtga
     5281 ttgataagtc tgtagctgac aagatgacca gtatgatgtt ggggactttc accaacggta
     5341 ccggtattag ttcatcgcct gcagactatg tcatggcagg gaaaactgga acaactgaag
     5401 cagttttcaa tccggagtac acaagtgacc agtgggtaat tggttatact ccggatgtag
     5461 tgattagcca ctggcttggc tttccgacca ctgatgaaaa tcactatcta gctggctcta
     5521 cttcaaacgg tgcagctcat gtctttagaa acattgccaa taccatttta ccttatacgc
     5581 caggaagtac ctttacggtt gaaaatgctt ataagcaaaa tggaattgca ccagccaata
     5641 caaaaagaca agtacaaacc aatgataata gccagacaga tgataatttg tctgatattc
     5701 gagggcgtgc gcaaagtcta gtagatgagg ctagccgggc tatctcagat gcgaagatta
     5761 aggaaaaggc tcaaacaata tgggattcga tagtcaatct atttcgctaa gatgcttgtc
     5821 aaagcctagc tttcttgtta taatggataa gatggaggcg ttatggcact aaaaaaagca
     5881 agcctagctt gtgcggtttg tggttcgaga aactattcaa tcaagatcag cggaaacccc
     5941 aagcctacac gactagaagt aaataaattt tgtaagcatt gtggcaagta cactacacac
     6001 agagaaacga gataggagag agcgatgcgt tttattggag atatttttag acttcttaaa
     6061 gacacaacat ggccaactcg caaggaaagc tggagagatt ttcgttctat catggaatac
     6121 acagctttct ttgtagtaat tatttacatt tttgaccagt tgattgtttc aggtttgatt
     6181 cgatttatta acatttttta gaagattagt ggagttaatt acactagaaa tcttctattt
     6241 atgaaaggaa atatcatgga tagttttgat aaaggatggt ttgttttaca aacttattct
     6301 ggttatgaaa ataaggtgaa agaaaatcta ttacaacgtg cacaaaccta caatatgttg
     6361 gataatattc tacgcgttga aattccaaca caaacagtgc aagttgaaaa aaatggaaag
     6421 agaaaagaag tagaagaaaa tcgctttcca ggttatgttc ttgtagaaat ggtcatgaca
     6481 gatgaagctt ggtttgttgt tcgaaacaca ccaaatgtta caggatttgt cggatcacac
     6541 gggaacagat caaaaccaac tccattattg gaacaagaaa ttcgtgacat tttggtatct
     6601 atgggacaaa ctgttcaaga atttgatttc gatgttgaga ttggtcaaac tgtacgtatt
     6661 attgatggtg cttttgcaga ctacactggt aagattacag aaattgataa taacaaagtg
     6721 aaaatgatta tctctatgtt tggtaatgac acagttgcag aagtaaacct aaaccaaatc
     6781 gcagaattat aa
//
LOCUS       DAVBNO010000080         6748 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVBNO010000080 DAVBNO010000000
VERSION     DAVBNO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.080"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(58..384)
                     /locus_tag="VQ846_001926"
     CDS             complement(58..384)
                     /locus_tag="VQ846_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET3076453.1"
                     /translation="MVEYISRKYFLTEEEFQAEKICNQSLVELFQKKYSWHSLNDFGS
                     STYDKNYASIFYEYWRSFLTVDKLVENLGSVQAVLDSYHLWANTEKTFPLLDWFVQQK
                     LIEKEI"
     gene            complement(351..635)
                     /locus_tag="VQ846_001927"
     CDS             complement(351..635)
                     /locus_tag="VQ846_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET3076454.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMTLISGSKRGWLNILVASIS"
     gene            complement(908..1750)
                     /locus_tag="VQ846_001928"
     CDS             complement(908..1750)
                     /locus_tag="VQ846_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET3076455.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALVWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(1864..3255)
                     /locus_tag="VQ846_001929"
     CDS             complement(1864..3255)
                     /locus_tag="VQ846_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET3076456.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIDQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(3490..3867)
                     /locus_tag="VQ846_001930"
     CDS             complement(3490..3867)
                     /locus_tag="VQ846_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET3076457.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIALV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            4082..5059
                     /locus_tag="VQ846_001931"
     CDS             4082..5059
                     /locus_tag="VQ846_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET3076458.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLTDKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYKTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            5056..6138
                     /locus_tag="VQ846_001932"
     CDS             5056..6138
                     /locus_tag="VQ846_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET3076459.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRIGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(6202..6748)
                     /locus_tag="VQ846_001933"
                     /pseudo
     CDS             complement(6202..6748)
                     /locus_tag="VQ846_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2039 a         1483 c         1137 g         2089 t
ORIGIN      
        1 tctgccactg atgcagtgaa atgtcaattt cttagacatt gtttactcct ttagttttta
       61 tatttctttt tcaattaatt tctgctgaac aaaccaatct aacaagggaa aagttttttc
      121 tgtatttgcc cataaatgat aagaatctaa gaccgcttgt acactaccta aattttctac
      181 caacttatct actgtcaaaa agctgcgcca gtattcataa aaaatacttg catagttctt
      241 atcataagtc gaagaaccaa aatcattcaa tgaatgccaa ctatacttct tctgaaaaag
      301 ttctacgaga gattgattac aaattttttc cgcttgaaat tcctcttctg tcaagaaata
      361 cttgcgacta atatattcaa ccatcccctc ttcgaaccag atataagagt cataaccatt
      421 cgtctattat ttgtataggc tggaacagcg ctttccctaa taatctgtgt agcagcatta
      481 aaatctgacc aaataacggc ttgcggtaaa tcacagactg caagttcatt ctttaaaaaa
      541 gattgataat ctttcaactt ttcagtattt cttactacaa aatcacgaaa tgtagctagt
      601 tgactatcgt cttttacaag ataaaggttg tccacggctc tcctttcttt cacacaatag
      661 actgaataag caaaagaacc acatctgaca atcctaggtt gatttaacct aggggcgtca
      721 gaaacgcggt tccaccctaa tttattactt cattgttttt gaaaatacta cagaaagcgc
      781 cagctcatta ttttgcccta cttggctccc actgtcccaa gctcgcttga agaacgccat
      841 aagactttct ttcctgctga ctattatatc aaaaattaga ataatgtaca attcttttta
      901 tgattttcta gaaatccaca tcatcaactc gtggagcacc cgattgaaca gcaatcgttt
      961 ttaaggtttc tctctcctca tgactcagtt caattccaaa gcaatcaaga ttagcttgaa
     1021 tacgagaggt tgtgacagat tttggaagtg gtaaaaatcc ttctgccaag ctccagacca
     1081 aggctatctg agcaatcgat tttccgtgat ttgctgcgat ttcttgcact tgcttgctat
     1141 caaatagttc tccctgacca aaaggccccc aagcttccaa taaaattccc ttttcacgac
     1201 agtaagctac gacttgttct tgatacacac ctggcgccaa gcgaacttga ttgaccgcag
     1261 gaacgatagt tgcagtttca agcaaggcat ccaaatgatg gggaagaaaa ttgctaacgc
     1321 cgatagcacg gattttccct tcttgataga ggtcttccat cgctctccaa acttccgcat
     1381 tgcgagtttt ccatgcgtca ttttctctga gcggttttgg gttcggccaa tgaatcaaat
     1441 acaaatccaa ataatccaag cccagttttt ctatagattt ttccaaagct tgacgagttt
     1501 gctcataggt ttgctgacta ttccaaagct tggtagttac gaacatttct tcacgtggaa
     1561 ctccgctatc tttgattgct tgaccaacac tttcttcatt ctgataaatc gccgccgtat
     1621 caatatgacg ataaccagcc ttcaaggctt ctaacactgc acgataggct tcttctccat
     1681 ccttagcctt aaaagttcca aatcccaata ctggaatttc tactccatta tttaattgat
     1741 aggtattcat atgcttctcc ttctatttta ttcatcaatt atagcataaa acatttttta
     1801 agtagtacta tattctgtaa aaaataagtc tgtcaaatat ctaacagacc tattttttct
     1861 tgcttattca tcacgactat agtacagctc atgagctttt aggcgagtat tgagcatctc
     1921 tggtatggtc acaaaggtat aaccttgatt tttcaaatac tcaatgaccc ttggcaaggc
     1981 attgactgtc ggactgtgaa tatcatgcat caaaacgata gagccgttag ctacttgatg
     2041 ctgaatttct gtcaaaatag atgcttcatt tttactcttc cagtccagac tatccacatc
     2101 ccacatgata aagctcaaat ccaagctatt gcgaatatca tctgtaatag caccataagg
     2161 tggacgcatg agtttagaac tagaacccag cactttagtt agcacatcct cagtatcagt
     2221 aatctgcttt ttagcttcat caagagagag ttgcgagaga atcggatggc tccaactatg
     2281 gtttccaaca acatgacctt cagattttat cctcttcacc aagtcctcat tcccagaaac
     2341 atttttccca agcacaaaga aagtcgcttt aatatcatat ttagctaggg tctctaatac
     2401 ctgcggggtc gttgctggat ttggaccatc atcaaaggtt agagcgacaa ctttttgatg
     2461 tttcttatca aagtaagatt ggtacaaggc cgcatctttt tcgagtaagt acgaagattg
     2521 gataacatca aagaaagcag atactggcaa ggctatctct tctaaatttt caaccacagg
     2581 acttggataa aggataatct gactatcctt gtaatcaaaa ttccatgcag acaagtcttg
     2641 gtcagagaag ttttttacaa tctgctcact ctggtcttga tctatttttt tatcctctat
     2701 gaaggaggtc aactctttta tcagctgttc cttagcctta ctagcatctg aaaacagttg
     2761 gtcaagtgta aaaggttgcc catcttctgt taagtgaacc tttcctagac tggtcttttc
     2821 agtctcttca atcttaaaag caactaaatc atagatttgt ttggtcacat tacgattaac
     2881 gactcccttt aaacctgact cttcttgctc tgtatagtag aaaacaagat tgtccttact
     2941 ttctagcttg tccttaacat cttgatttat cagctcccta acagaggaaa tcactttctc
     3001 cccttggaga ggataatagg caatcacttc ggcttgcccc tgacgaaaat gctccttctg
     3061 atttccctca ctcaattgat catctttctc ttttttgagc gattcaatct tttgctcaaa
     3121 acttttttgc tggtagatct taaaagcaat aaatccgcct aataggcaaa tagaaattgc
     3181 taaaatacca atcaaagcca ataatacatg tctcgttttc ccgtgtctgc cacgtcctag
     3241 tctactttta ttcatattta tatcataaca aatttgagca ataatttcaa tgatacaatt
     3301 tggcaatcca caattcctct agcaagccaa agcatcttac atcaactcca acatccgtat
     3361 ctgtagagat tatgtagttt tctatctatg gaatttctag tagattgaag ctagaatagt
     3421 agacctatac ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatc gatttgtcct
     3481 gttctcattt cattttacta taaaactatg aaaagctagg tcatctgaga agtaattact
     3541 atcgacaatt gcgatagcgt atactagtgc gatttgtttg tagctctggt gggtatcacc
     3601 ttcacgttgg cgaatttttt ctaggttttg attaacctga ctgcacagat aagcccatag
     3661 gcgattgatg aaaaaattct gatgatgaac ctgaatttcg atgataactt ggattccatt
     3721 atctaactcc gccaagacgt ctatactagt atagaaatct tgcgctgagt agggcatgga
     3781 aggcaagacg tgaatgttac ttccctccaa aatcgtcaca ttttttgctg gcaagtccag
     3841 catatcgcgg ataaattgac aaatcattag ttgggctgat acctggatgt ctgagaatcc
     3901 tccttttcct ccttctatta tagcacattt ttaaatctag gtttgctaga ttctatgctt
     3961 ctatctattt attcggaaaa gaaagctaat agcttatttg aaattttctc ctctctgttg
     4021 gatagtcccg tctatctatg ttataatgaa agaaggattt aaaatcggaa aaggagtatt
     4081 tatgctaaaa ttaggtgtca tcggaactgg cgctatcagc catcatttca tagaagcagc
     4141 ccataccagt ggagaatacc aactggtcgc aatctattct agaaaactag aaactgcagc
     4201 aacctttgct tctcgctatc agaatatcca actattcgat caattagaag tcttcttcaa
     4261 atcttctttt gatttagtct atatcgccag tccaaactcc ttacattttg ctcaggcaaa
     4321 agctgccttg tctgctggta aacatgttat tctcgaaaag ccagctgtca ctcaaccaca
     4381 agaatggttt gatttgattc aaacagctga aaaaaataac tgttttatct ttgaagcagc
     4441 tcgtaattac cacgagaaag cctttactac tatcaaaaac tttttaacag ataagcaagt
     4501 gttaggagca gatttcaact atgccaaata ctcttccaag atgcctgact tgttggctgg
     4561 acaaacacca aatgtctttt cagaccgttt tgctggtgga gccctcatgg atttggggat
     4621 ttatcccctc tatgctgctg ttcgtctttt tgaaaaagct aatgacgcga cttaccatgc
     4681 tcaacagctt gacaatagca ttgacctaaa tggagatggt atcctcttct acccagacta
     4741 tcaagttcac atcaaggctg gaaaaaacat cacttccaat cttccttgcg agatttatac
     4801 aacagatgga accttgactc tcaacacgat tgagcatatt cgctcagcta tttttaccga
     4861 ccaccaagga aatcaagtcc agcttcctat ccaacaggct cctcatacga tgactgagga
     4921 agtcgctgca tttgcacaca tgatccagca accagatctg aatctctaca aaacttggct
     4981 gtatgatgca ggctctgttc atgagctact atataccatg cgccagactg ctggcattag
     5041 atttgaggca gaaaaatgaa aaccaaacta ccgactgaat ggcaagaact gagtgaccaa
     5101 ctcggtttcc aagaattcac ccccattcaa actcaactat ttgagcccct tcttgctgga
     5161 gaaaacctcc taggagtgag ccaaaccgga actggtaaga ccctagcgta tctcctacca
     5221 agtcttctca gactacaaaa gaaaaaagcc caacaactct tgattctagc accaaacacg
     5281 gaacttgctg gacagatttt tgatgtatgt aaaacgtggg cagaagctat cggattgact
     5341 gctcaactct tcctttcagg ttcaagtcag aaacgacaaa ttgaacgtct taaaaagggg
     5401 ccagaaattc tgattggaac tcctggccgt atctttgaac tcattaaact taaaaaaatc
     5461 aagatgatga atgtggaaac catcatcctg gatgaatttg accaattact agatgattct
     5521 cagattcact ttgtagaaaa aatcactcac tacgcacctc gtgaccacca actggtctac
     5581 atgagtgcca cgaccaagtt tgaccaagaa aagattgtgc cgaatacacg cactattgat
     5641 ctctctgacc aaaaattgga caatatccag catttctaca tgcaggtaga ccaacgtcat
     5701 cgagtggata tgctacgaaa actggctcat gtagaggatt tccgtggtct agtcttcttc
     5761 aatagcctgt cagaccttgg gaatgccgag gaaaaactac agtatcgtga tatattgact
     5821 gtttccctcg ctagtgatgt taatgttaaa tttagaaaaa tcatcttaga aaagtttaaa
     5881 gataaccaac taaccctgct tcttgcaact gaccttttgg ctcgtggaat tgatatcgat
     5941 agcctagaat gtgtcgtaaa ctttgatatt cctagagata gcgaaaccta cacacaccgt
     6001 gctggtcgta ctggccgtat aggcaaggaa ggatatgtta ttactctcgt cactcatcca
     6061 gaagaaatca aaaaacttaa aaagtttgca agtatccgag aaattgtcct aaaaaaccaa
     6121 gaactctata tcaaataaag ccccattgct ctatacacat ccagtgtatg gagctttttt
     6181 ataagcaatt caattttata ctcatctgct ttcaaaaagc attctattcc atctccgatt
     6241 aacgatggac tttatcacct ccttctccag tccttgtatg acatcttgga gttgattcat
     6301 gacatcttcc aaagttcgaa aggctttatt cttaaatcca cgtttacgaa tctctttcca
     6361 cacttgttca atgggttcat ctctggtgtg tatggaggaa taaaggtaaa atcaatatta
     6421 gtcggaatct ttaaggtact tgatttatgc catatagcat tgtccataac gagtaaaaaa
     6481 taatcatctg gataagcttg tgaaagctct tctaaaaagg cgttcatcca ctcagtatta
     6541 catccaccag ctattaagaa aaatgattcg cctgtatggg catcaacagc tccataacaa
     6601 tagcgaaatt ctcgtatata gtgactatgg acatgtggac ctactcctat tggagaccaa
     6661 caagatccca gtttactgat tctaccgaaa ccagcctcat cttggtacat caagcgaacc
     6721 ttatgaaaag gtctactggt tttaaagc
//
LOCUS       DAVBNO010000081         6726 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVBNO010000081 DAVBNO010000000
VERSION     DAVBNO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.081"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            138..860
                     /locus_tag="VQ846_001934"
     CDS             138..860
                     /locus_tag="VQ846_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET3076460.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            1077..2411
                     /gene="pepC"
                     /locus_tag="VQ846_001935"
     CDS             1077..2411
                     /gene="pepC"
                     /locus_tag="VQ846_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET3076461.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSR
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAVLNKLLRQDAQILRDLLVSGADQVTVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(2467..3378)
                     /locus_tag="VQ846_001936"
     CDS             complement(2467..3378)
                     /locus_tag="VQ846_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET3076462.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(3402..4205)
                     /locus_tag="VQ846_001937"
     CDS             complement(3402..4205)
                     /locus_tag="VQ846_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET3076463.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(4233..5222)
                     /gene="manX"
                     /locus_tag="VQ846_001938"
     CDS             complement(4233..5222)
                     /gene="manX"
                     /locus_tag="VQ846_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET3076464.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(5455..6474)
                     /gene="adhP"
                     /locus_tag="VQ846_001939"
     CDS             complement(5455..6474)
                     /gene="adhP"
                     /locus_tag="VQ846_001939"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET3076465.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKI
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         2018 a         1388 c         1477 g         1843 t
ORIGIN      
        1 agatagaact gacgaagtca gtaacatata tacggcaagg cgacgttgac gcggtttgaa
       61 gagatttttg aagagtatga aacgaaaagg aggttggtca ttgtactagc ctccttttga
      121 tatgatataa tagttctatg agattagata aatttttagt tgcctgtgcg gtgggaagtc
      181 ggactgaggt caaaaacttg ctcaaggctg ggcgcgtgac tgtaaatggt aaaaaagaaa
      241 aatcagctaa attgcagatt gatgaaaaaa tagatgagat tcgctttgat gggcaagtgt
      301 tggagtatga agagtttgtc tactacatga tgaacaagcc caaaggagtt atctcagcga
      361 ctgaggatcc caagcacaga accgttctgg acttgttgga tgacttggcg cggagtaagg
      421 aagttttccc agtaggacgc ttggatattg acacgcatgg tcttttgctc ttgaccaatg
      481 atggtcagct ggctcatgtt cttctttcgc ccaagcgtca tgtggacaag acttatctgg
      541 cacaagtcaa gggaatcatg acccaagaag atgtggagac atttgctgag ggtattcctc
      601 tcaaagactt tacctgtcaa cccgctatac tggagcttgt atccatagat acagaaaaga
      661 atcaaagcca aatccgtgtg accattgcag aagggaagtt tcatcagatc aagcgtatgg
      721 tgggctactg tggcaaggaa gtggtggact tgcaacgatt gactatggga actttagtat
      781 tagatgagaa cctggaacga ggggaatggc gtcgcttgac caaggaagaa ttagaaattc
      841 tccgcgctaa tattatttaa tttagtttaa tactcaatga aaatcaaaga gcaaactagg
      901 aaactagccg caggctgtac ttgagtacgg caaggcgacg ttgacgtggt ttgaagagat
      961 tttcgaagag tataaattcg aaaaaggtga gggacaatgt cctcgccttt tatgtttttt
     1021 agttgcttcc tttgtgaaaa gagttataat agactgtaga ataaaaggag gaatctatga
     1081 acgcgattca agaatcattt actgataaac tatttgccaa ctatgaagca aatgtaaaat
     1141 accaagcgat tgaaaatgct gccagccaca acggaatttt tgcagctctg gaacgtcgcc
     1201 aaagccgtgt agacaacaca cctgttttct cattggattt aaccaaggac aaagtcacta
     1261 accagaaagc gtctggtcgt tgctggatgt ttgcggctct caacaccttc cgccacaaac
     1321 tcatctcgca atacaaattg gagaactttg agttgtcaca agcccacact ttcttctggg
     1381 ataagtatga gaaatcaaac tggttcttgg agcaagtcat tgcgacttca gaccaagaat
     1441 tgactagccg caaggttagc ttcttactcc aaacacctca acaagatggc ggtcaatggg
     1501 atatggtcgt ttccctcttt gaaaaatacg gtgtcgtgcc taagtcagtt tatcctgagt
     1561 ctgtttcatc tagcagcagt cgtgagctaa atgcggtcct taataaattg cttcgtcaag
     1621 atgctcaaat cttgcgtgat ttgcttgttt ctggtgcaga tcaagtgact gttcaagcta
     1681 agaaagaaga cctcttgcaa gaaatcttta actttcttgc tatgtcatta ggacttccac
     1741 cacgcaagtt tgactttgct tatcgcgata aagataacaa ctacaaaagt gaaaaaggaa
     1801 tcacaccaca agagttctac aagaaatatg tcaatcttcc tttagaagac tacgtttctg
     1861 ttatcaatgc tccaactgct gataaacctt acggaaaatc ttacacagtt gagatgttgg
     1921 ggaatgtggt tggtagccgt gcagttcgtt acatcaacgt tccaatggag cgcttgaaag
     1981 aattggcgat tgcccaaatg caagcaggtg agactgtttg gtttggttct gatgtcggcc
     2041 agctcagtaa ccgtaaggct ggaatccttg cgacagatgt ttatgacttt gaatcaagca
     2101 tggacattaa acttactcaa gacaaggctg gacgtttgga ctatagcgaa agcttgatga
     2161 cccacgccat ggtcttgaca ggtgttgact tggacgaaaa tggtaaatca accaagtgga
     2221 aggttgaaaa ctcatgggga gacaaggtcg gtacagatgg ttactttgtt gcctcagacg
     2281 cttggatgga cgaatacaca taccaaatcg ttgttcgtaa ggaattgctg acagcagaag
     2341 aacaagctgc ctatggagca gaaccaattg tacttgcacc atgggatcca atgggagcct
     2401 tggctgaata aaagcataga aaaaaggaat cagattttag aacctggttc ctttttagtt
     2461 gcttgattac atgatgtgaa gaacatgtgc cacaataccc actgcgaaga gagcaaggat
     2521 aatagtgatt ggagatactt ttttcttaag taagtacatg caaagtaaag taaggagtag
     2581 tcctgataat ccaggaatca acatatccaa gttttgttgg aaagtagtaa ctttttcagg
     2641 agtttgagac aatccttgtc ctacttgtgc gaatgcttct tggatacctt tagacccttc
     2701 tggcaattta tcccaatgga tataagcctt ttcatctagt tgaactttag aaacatcgaa
     2761 agcaaattta atatttaccc agcgttgaac aaggacagca agaatgaaca tcccaaggat
     2821 agaagctcct ttagtgatat cttgaaggat accaccagac atatctttag tgatttctga
     2881 tccagcctta tatccaatct cttgaacata ccacaagaat gacatacgaa tcaagttcca
     2941 tgcaacaaag aagaggagtg gacccaagat attgccagta agggcaagtg aagcaccgag
     3001 agatccaagg attgggcgca ctgtaaacca gaatactggg tcaccgatac cagcaagagg
     3061 tcccatcata ccgattttaa ccccttggat agcagcgtca tcgatttcca caccgttagc
     3121 acgttcttct tcaagcgcaa gagtaacccc catgactgga gcagctacgt atggatgagt
     3181 gttgaagaac tcaaggtgac gctcaagagc agcgatttga tcttcttttt tagtatagag
     3241 ttttttgata gctggaatga gtgtataagc ccagcccaag ttttgcatcc gttcaaagtt
     3301 ccaagaccct tgtaagaagg ttgaacgcca ccaaactttt ttacgatctg atttagttaa
     3361 ttgaagtttt tcagtcatga tgttttcagt cctttcttat cttagtagtc ttctaggata
     3421 tcgccgattg ggtcgttaga agttgcggct cctccgccac catttccacc agttttagaa
     3481 aggtgaaggt agataagagc gatagcaacg ccgatagcac cgaatccgat tagagtaata
     3541 tctgacacag cagcgagaac gaaaccaaga gcgaagaatg gccatacttc acgagttgcc
     3601 atcatgttga taaccatggc gtaaccaacg gcaacgacca taccaccacc gatagccata
     3661 ccatctttga gccagtctgg catggcacta aggatacttt gtacagtttc agttggtacc
     3721 ataaggagaa gagctgcagg aagcgcgata cgaagtcctt ggaaaagtag cgcgatgaaa
     3781 tgcgcacgct ccacagcgcc gaagtcacct tttttagcgg cagcatctgc agtatgaacc
     3841 aaaccaactg aaattgtacg aacaatcatt gtcaagaaaa gtccagctac agcaagaggg
     3901 atagcaaccg cttgggcaac accgatacca gtcttggtaa agtcaccacc aagaaccatg
     3961 ataatggcag cagcgacaga agcaagtgca gcatcaggag cgatagcagc accgatattt
     4021 gaccaaccaa gggcaatcat ttgaagcgat ccaccgagga taatccctgc ttccaagtga
     4081 cctgttacaa gcccaataag ggtacaggct acaagtggtt ggtgaaattg gaactggtcg
     4141 aggatgcctt caagacctgc aaagaaggct acaacgacta ctaaaaccat agaaataata
     4201 gacatgttta aaatcctttc ataaataatg gcttatttga cattggcttt gttaatcaag
     4261 tcaaacaaat cttttttaga atcatttggt actttacgga catcaaattc aacacccaag
     4321 tcacgcattt tttcaaatgt agcaacgtct tctttgtcca tagacaaaac ggtattgacc
     4381 aatgttttac ctgttgagtg agccatagaa ccaacattaa gagtcttgat tggcacgccg
     4441 ccttcgatgg cacgaagggc atcttgaggt gtttcaaaca agataagggc atgtgtttct
     4501 ccaaaacgtg ggtcttttga aatctcaatc agtttttgaa ttggaaccac gttagccttg
     4561 acattacctg gagctgcttg tttaatcaat tctttacgaa ggtcgtcttt agccacgtta
     4621 tctgaagcaa cgatgatacg atttgctttt gaatctggag tccaagcagt tgcaacctga
     4681 ccgtgaagta gacgtgtgtc aagacgggca agattgattt tcaatttacc gtctccgata
     4741 acagttcctt ctgggatagc agtttgggca actggagcag ctgcagcgct tgcaacttct
     4801 tcgactggat ttagctcttc tggaagagct ttgatgccat ctttggcttc tttaatgata
     4861 ttagcagcga ctttttctac acctgcagca gcgtccatga ggcgctctgt gtaggcttga
     4921 atcaacatcg gtaagttaag tcctgtgatg atggcaaact tacgctcagg attttctccc
     4981 atcacgcgac tagcttggtt aaatggagaa ccactccaaa ggtcagccaa aactagaacc
     5041 tcatcttctg cgtcaaatgc agcaacagcg ttattaaact tagcgtatag atcatcagga
     5101 ccttcatttg gcataaaggt cacaacttga accttttctt gttcaccaaa gatcatagat
     5161 cctgactgat gaatacccgc agcaaattcg ccgtggctcg caataatgat tccgatactc
     5221 attattgtca ttcctccttt tttgttttag ttttcattta agaaaacttt aagactgttt
     5281 aagtataaac cgttttcata aaaaaagcaa ctatttatca taaaaaatta aaaaattttt
     5341 atttttgtat gttgatatgt tgagtttttg aggagtaatc ttgaattctt atttaaaatt
     5401 taatataaaa aagttggaag aagcttccaa cttttaaaat aggtttctat tagattagtg
     5461 ggtgaagtcg agtaccatac gtccttggat ttggcctttt tccatttcgt cgaaaatggc
     5521 aacagcatct tctactggac gtttttgaac aactgggact accagacctt ctgcaccaaa
     5581 ttggaaggct tcttctaagt ctttacgagt tccgacaaga gaaccgatga cttggattcc
     5641 atcgaggact gttttaacga tgctgagttc catcatttca gaaggaagac caacagcgac
     5701 gacgcgacca ccagcacgaa tggagtcaac agcctggttg aaggcaactt tagacacagc
     5761 agttacgaca gctgaatgag ctcctccatc agttttttct ttaatgagtc cagctacatc
     5821 ttcaacttcg aggccgttaa tcacaatgtc agcgcctact tcttttgcaa gggcaagttt
     5881 gtcattgttg atatcgactg cgataacatg agcattgaat acttttttag cgtattgaac
     5941 agcgaggtta ccaagtccac cagcaccgta aagaacaacc cattggcctg gttcaatttt
     6001 tgcttctttg atagctttat aggttgttac tccagcacat gtgatagaag aagcttgggc
     6061 tggatcaagt ccgtcaggaa ctttgacagc atagtcagca gttacgatac attgttcagc
     6121 cataccaccg tctactgagt agccagcatt tttcactgta cggcaaaggg tttcgcgacc
     6181 agttgtacag tattcgcaag tgccacatcc ttcaaagaac caagcaacgc tgacgcggtc
     6241 accgacttta aggcttttca catctggagc aatctcttta acgataccga taccttcgtg
     6301 cccaagaaca cgtcctggga cttgaccaaa gtcaccatga gcaacgtgga ggtcggtgtg
     6361 gcaaacacca cagtattcaa cttctacaag tgcttcccca gtttcaagtg gacggagtac
     6421 tttttcttca atagcaacac cagtgctttc tggatttaca acaacagctt tcatagctat
     6481 cctccttgat attgaccgag aacagggaca acgggactgg attgattttt tgtcaacaga
     6541 gtcgagtgtg acgagctctc ttgtatttat atgtgaagta tatcacaaaa gaaagccttt
     6601 tccaaggttt tggagacaaa aagtagaaca atcgattaac tggttgatga gactgtgaaa
     6661 agagcacaaa gaccagcttg caagctggtc atactcttcg aaaatctctt caaaccacgt
     6721 cagctt
//
LOCUS       DAVBNO010000082         6627 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVBNO010000082 DAVBNO010000000
VERSION     DAVBNO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.082"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            170..370
                     /locus_tag="VQ846_001940"
     CDS             170..370
                     /locus_tag="VQ846_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076466.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDQK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            412..816
                     /locus_tag="VQ846_001941"
     CDS             412..816
                     /locus_tag="VQ846_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076467.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1047..1754
                     /gene="ccdA2"
                     /locus_tag="VQ846_001942"
     CDS             1047..1754
                     /gene="ccdA2"
                     /locus_tag="VQ846_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET3076468.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1765..2322
                     /gene="sdbB"
                     /locus_tag="VQ846_001943"
     CDS             1765..2322
                     /gene="sdbB"
                     /locus_tag="VQ846_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET3076469.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDASNFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAETAFKEMN"
     gene            2697..4088
                     /locus_tag="VQ846_001944"
     CDS             2697..4088
                     /locus_tag="VQ846_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET3076470.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIIAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLILLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4231..5148
                     /gene="adcAII"
                     /locus_tag="VQ846_001945"
     CDS             4231..5148
                     /gene="adcAII"
                     /locus_tag="VQ846_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET3076471.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDSDVFVYHSHTLESWAGILDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLEADPQNDKTYLENLEENMSVLAEELK"
     gene            5156..>6627
                     /gene="phtB"
                     /locus_tag="VQ846_001946"
     CDS             5156..>6627
                     /gene="phtB"
                     /locus_tag="VQ846_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HET3076472.1"
                     /translation="MKINKKYVAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQEH
                     SHNHGGGSNDQAVVAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSD
                     LSASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISS
                     LLRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRI
                     ARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPSPQPAPSNPIDEKLVKEAVRKVGDGY
                     VFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYD
                     LLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLV"
BASE COUNT         2032 a         1215 c         1410 g         1970 t
ORIGIN      
        1 gtaaaatgaa ataagaacag gacaaatcga tcaggacagt aaaatcgaat tctaacaatg
       61 ttttagaagt ccatatgtac tattctagtt tcaatatatc ataactgttc ctagtcaacc
      121 tgcaagccct atcccagctc caagctcagc tccgactcaa gatcctagag tggtagcaag
      181 cgcttcggct agctcaacga gcacacaagc tcaagagcaa gttgacaagt ctgaacttcg
      241 tgccttgagt caagagctag accaacgctt gaaagctttg gcaacagtat ctgatcagaa
      301 aatcgatgca accaaggctg tcctcctaga tgctcaaaaa gctccggaag atagtgcctt
      361 gacagagtaa ggtctgcata ctgcagtaga gtctgtgaag gctgggctgc cttggactct
      421 ctcaaagatg tgaaagcaaa tgctagcgac agcaagcctg cacaggacaa gaaggatgca
      481 aaacaaggaa cggaagatag taaggattca gataagatga ctgaaacaaa ctcagttccg
      541 gcaggagtga ttgtggtcag tctacttgcc ctcctaggcg tgattgcctt ctggctgatt
      601 cgccgtaaga aagagtcaga aatccagcaa ttaagcacgg aattgatcaa ggttctagga
      661 cagctagatg cagaaaaagc ggataaaaaa gtccttgcca aagcccaaaa ccttctccaa
      721 gaaacccttg atttcgtgaa agaagaaaat ggctcagcag agacagaaac taaactagta
      781 gaggagctta aagcaatcct tgacaaactc aagtaaaaca tgatttatga aatgtcagac
      841 ctaaaagaca ttttcgttca caaaagcagc gatgagtaat ccttgtcgtt gctttttggc
      901 tattttgggg gataagatcc gccttgacta gcaaaagaaa agattggtcc aatagtgccc
      961 gatacgattt tgcgtagcag aaagcttaca aataaaattg aatatagtaa aataggatta
     1021 gaattattaa gaaagttggt tctttattgg aaacgatagt atttttaatc tctgtttttc
     1081 tagcaggtgt tttatccttt ttttctcctt gtatttttcc tcttctgcca gtctatgctg
     1141 ggattttatt ggatgatcaa gaaagtgcaa aaagcttttc tttgtttggg agaaaggttc
     1201 tctggtcagg cttgattcga acactttgct ttatcgctgg tatctctctc attttcttta
     1261 ttctaggctt tggtgctggt tactttggtc atattctcta tgcaaattgg tttcgatatg
     1321 gcatgggagc tattattatc attttgggtc ttcaccagat ggaaattttt catttgaaga
     1381 aattagaagt tcaaaaaagt tttaccttta aaaaatcaga ttctaatcgt tattggtcag
     1441 cttttttact tggtattacc tttagctttg gttggacacc ttgtattggt ccagttttaa
     1501 gttctgtttt agcacttgcg gcttctggag gcaatggcgc ttggcaagga gcgatttata
     1561 ctctcattta cactctgggc atggcccttc ctttcttggt attggcacta gcttcaggtc
     1621 tagtcatgcc atattttagt aaaatcaagc gtcatatgat gctactaaag aaaattggtg
     1681 gtttcctcat tgttttaatg ggaattttgt tactattagg acaagtaaat gttctagctg
     1741 gaatttttga ataaaggagg acaaatgaaa aaagtaatgt ttgctggctt aagtctcttg
     1801 tcattagttg tattgatggc ctgtggtgag gaagaaacta aaaagactca agcagcacaa
     1861 cagccaaaac aacaaacgac tgtacaacaa attgctgttg gaaaagatgc ttcaaacttc
     1921 acattgcaat ccatggatgg caaagaagtt aagttatctg attttaaggg taaaaaggtt
     1981 tacttgaagt tttgggcttc atggtgcggt ccatgcaaga aaagtatgcc agagttgatg
     2041 gaactagcgg cgaaaccaga tcgtgatttc gaaattctta ctgtcattgc accaggaatt
     2101 caaggtgaaa aaactgttga gcaattccca caatggttcc aggaacaagg atataaggat
     2161 atcccagttc tttatgatac caaagcaacc accttccaag cttatcaaat tcgaagcatt
     2221 cctacagaat atttaattga tagccaagga aagattggaa agattcaatt tggtgctatc
     2281 agtaatgcgg atgcagaaac agcatttaaa gaaatgaact agaatcgata aaatctgatt
     2341 acaagatgta gtcagatttt ggtaacttct aaaactaact tccagtttcc cacaacctag
     2401 cttttagtat gagaaaaacg cgtgacatca gtggaaatcc gtcttagact aacttccact
     2461 ggttttcttt ttcttgatat ataagggttc aaaagcgaaa agactcagaa aaaagtgcac
     2521 gacaaatagc cctaaaacag agtcgtttta gagtaacttc cagttttggg agagagatgg
     2581 aagttacttt gagaagttac ggtagtcaga tttttttaga aaaacatttt ataaatattc
     2641 acatatctcc tatatttatg gtaaaataga atcatcagtt tattttggag tcaaagatga
     2701 atatatttag aacaaagaat gttagtttag ataaaacaga gatgcatagg catttgaagt
     2761 tatgggattt gattttgctg ggtattggag ccatggtagg gacaggtgtc tttacaatca
     2821 caggtactgc agctgctaca cttgctggtc cagcactagt gatttcaatc gttatttctg
     2881 ccttgtgtgt gggattatca gccctctttt ttgcagaatt tgcctcgcga gtacccgcta
     2941 caggaggtgc ctatagttac ctctatgcta tcttaggaga attccctgcc tggttggctg
     3001 gttggttaac catgatggag ttcatgacag ccatatcagg cgtagcttcg ggttgggcag
     3061 cttattttaa aggcttgctc tctcaatacg ggatagccct tcctcaggct ttaaatggta
     3121 cctttaatcc tcaagcaggg acatttgttg atttattgcc tattcttgtc ttggtcttgg
     3181 tgacttcgct tgttttacta aatgccaagg cagccttacg ctttaattcg attctagtta
     3241 ttttgaaatt ttccgcttta gctctctttg ttttagtagg aatttggaat attaagtttg
     3301 ataattggag caattttgct ccttacggtt ttggacaaat ctatggagct agtactggta
     3361 ttatagctgg tgcgtccttg atgttcttcg gttttttggg atttgaatcc atctctatgg
     3421 ctgtagatga agtcaagact cctcaaaaaa atattcctag agggattgtc ttatcgcttt
     3481 ctatcgtaac cattctctat gccttggtga cccttgtttt gactggtgtg gttcactata
     3541 gtcatttaaa tgtcgatgat gccgttgcct ttgcccttcg tagtgttggg attagttggg
     3601 cagccaacta tgtgtcatta gtggctatct tgaccttgat tacagtttgt atttcgatga
     3661 cctatgccct atcgcgtatg atttacagtt tagcacgtga tggcttagtg cctgctgcct
     3721 ttaaggaact gacgaagact agcaagatac caaagaatgc tactatcttg acaggtctag
     3781 cttcagcagt agcagcagga atgttcccgc tagccagtat tgcagccttt ttaaatattt
     3841 gtaccttagc ctacttgatc ctgctggctt atggtctgat tcgcttacgg aaagaaaaag
     3901 gaatgcccaa agctggggaa tttaaaacac cattggtacc tctgttacca attttatcga
     3961 tcatcatttg tttatccttc atgttacagt ataatatgaa tacctggatt gctttcctag
     4021 ttgcgttgtt agtaggaagt attatttact tcacttatgg ctataagcat tctaccatag
     4081 aagaataaag tgaaagtcta ttgagttgtt gacactcagt agatttttat atgtgaaaga
     4141 aatttcaaat tttttctaaa aatatcttga cagattaaat tttcaggagt agaatattta
     4201 ctagttaatt aaatggttaa ggagttgttc atgaagaaac aaaatttatt tttagtcctg
     4261 ttaagtgtct ttcttttgtg cttgggggct tgtggtcaaa aggaaagtca gacaggaaag
     4321 gggatgaaaa ttgtgaccag tttttatcct atctacgcta tggttaagga agtatctggt
     4381 gacttgaatg atgttcggat gattcagtca agtagtggta ttcactcctt tgaaccttcg
     4441 gcaaatgata tcgcagccat ctatgattca gatgtctttg tttaccattc tcatacactc
     4501 gaatcttggg caggaattct ggatccaaat ctaaaaaaat ccaaagtgaa ggtcttagag
     4561 gcttctgagg gaatgacctt ggaacgtgtc cctggactag aggatgtgga agcaggggat
     4621 ggagttgatg aaaaaacgct ctatgaccct cacacatggc tagatcctga aaaagctgga
     4681 gaagaagccc aaattatcgc tgataaactt tcagaggtgg atagtgagca taaagagact
     4741 tatcagaaaa atgcgcaagc ctttatcaaa aaagctcagg aattgactaa gaaattccaa
     4801 ccaaaatttg aaaaagcgac tcagaaaaca tttgtaacac aacatacagc cttttcttat
     4861 ctagcgaaga gatttgggct taatcaactt ggtattgcag gcatctctcc tgaacaagaa
     4921 ccaagtccac gacaattaac agaaattcag gaatttgtta aaacctataa ggttaaaacg
     4981 atttttacag aaagtaatgc ttcttcaaaa gtagctgaaa ctcttgtcaa atcaacaggt
     5041 gtgggtctta aaactctgaa tcctttagag gcagacccac aaaatgacaa gacctattta
     5101 gaaaatctgg aagaaaatat gagtgttcta gcagaagaat taaagtgagg aaagaatgaa
     5161 aatcaataaa aaatatgtag caggttcagt ggcagttctt gccctaagtg tttgttccta
     5221 tgaacttggg cgttaccaag ctggtcagga taagaaagag tctaatcgag ttgcttatat
     5281 agatggtgat caggctggtc aaaaggcaga aaatttgaca ccagatgaag tcagtaagag
     5341 ggaggggatc aacgccgaac aaatcgtcat caagattacg gatcaaggtt atgtgacctc
     5401 tcatggagac cattatcatt actataatgg caaggtccct tatgatgcca tcatcagtga
     5461 agagctcctc atgaaagatc cgaattatca gttgaaggat tcagacattg tcaatgaaat
     5521 caagggtggt tatgtcatta aggtaaacgg taaatactat gtttacctta aggatgcagc
     5581 tcatgcggat aatattcgga caaaagaaga gattaaacgt cagaagcagg aacacagtca
     5641 taatcacggg ggtggttcta acgatcaagc agtagttgca gccagagccc aaggacgcta
     5701 caccacagat gatgggtata tcttcaatgc atctgatatc attgaggaca cgggtgatgc
     5761 ttatattgtc cctcacggca atcacttcca ctatattcct aagagtgatt tgtctgccag
     5821 tgaattagct gctgcccaag cctattggaa tgggaagcag ggatctcgtc cttcttcaag
     5881 ttctagtcat aatgcaaatc cagctcaacc aagattgtca gagaaccaca atctgactgt
     5941 cactccaact tatcatcaaa atcaagggga aaacatttca agccttttac gtgaattgta
     6001 tgctaaaccc ttatcagaac gccatgtgga atctgatggc cttattttcg acccagcgca
     6061 aatcacaagt cgaaccgcca gaggtgtagc tgtccctcat ggtaaccatt accactttat
     6121 cccttatgaa caaatgtctg aattggaaaa acgaattgct cgtattattc cccttcgtta
     6181 tcgttcaaac cattgggtac cagattcaag accagaacaa ccaagtccac aaccgactcc
     6241 agaacctagt ccaagtccgc aaccagctcc aagcaatcca attgatgaga aattggtcaa
     6301 agaagctgtt cgaaaagtag gcgatggtta tgtctttgag gagaatggag tttctcgtta
     6361 tatcccagcc aaggatcttt cagcagaaac agcagcaggc attgatagca aactggccaa
     6421 gcaggaaagt ttatctcata agctaggagc taagaaaacc gacctcccat ctagtgatag
     6481 agaattttac aataaggctt atgacttact agcaagaatt caccaagatt tacttgataa
     6541 taaaggtcga caagttgatt ttgaggcttt ggataacctg ttggaacgac tcaaggatgt
     6601 ctcaagtgat aaagtcaagt tagtgga
//
LOCUS       DAVBNO010000083         6207 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVBNO010000083 DAVBNO010000000
VERSION     DAVBNO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.083"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(482..707)
                     /locus_tag="VQ846_001947"
                     /pseudo
     CDS             complement(482..707)
                     /locus_tag="VQ846_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1555..2427
                     /locus_tag="VQ846_001948"
     CDS             1555..2427
                     /locus_tag="VQ846_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET3076473.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(2573..2917)
                     /locus_tag="VQ846_001949"
                     /pseudo
     CDS             complement(2573..2917)
                     /locus_tag="VQ846_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            3060..3371
                     /locus_tag="VQ846_001950"
     CDS             3060..3371
                     /locus_tag="VQ846_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076474.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKKREELRQIITTIFEQKMNKHSI
                     K"
     gene            3375..4091
                     /locus_tag="VQ846_001951"
     CDS             3375..4091
                     /locus_tag="VQ846_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET3076475.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(4221..5036)
                     /locus_tag="VQ846_001952"
     CDS             complement(4221..5036)
                     /locus_tag="VQ846_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET3076476.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(5002..5940)
                     /locus_tag="VQ846_001953"
     CDS             complement(5002..5940)
                     /locus_tag="VQ846_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET3076477.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMFFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIPYDSIFSFSWLF"
BASE COUNT         1960 a         1073 c         1251 g         1923 t
ORIGIN      
        1 aaataagata tgtacaaatt gattagaaag tcaaatgaat ttctacaaat gttttagtaa
       61 tcgcaatcta cttgtctaga ttcgatctga tatattttcg atttaatgat atggtattta
      121 aaacctccaa agtagcttgc tccattcttt tacttacgtg agtgtagatg ttatttactg
      181 ttttagcgtt tttgtgttcc actctaacca ttatagcatt cttctcagct agtgtactaa
      241 ggagtgtgtg cctgaaaata tgggaactaa ggggctggtt tatcggtttc tctagtttag
      301 tatttgcctt ttgcaaagtg atgttaaatg cctttctcta aattcacata tcactattgt
      361 ttaacaaaat ctaatctact ttaggtcact tattcttttt ttgaaatgta gaatgaactt
      421 tttcaaagtt tttcgaatct tttaaaatct gtttgtttta tatcgccatt ctcccccttt
      481 tttaattctc cctatatagc ctgatagctt tcccgatggt acgaatatgg ttgctttcgt
      541 ctaggtggat gtcggggtat tcgggattga gtttttttga ggcagccttg gcggagtttc
      601 ttgacatagt tagtgccgtc tacttggaag atgccgatgg tattatagtc aatctgtggg
      661 gtattcttga taaataggta gtcgctgttt cttatctttg gctccatgga cttgctgacg
      721 acataagcga ttgggtcgta gtcgtctggg ataatggaaa ctccataact aaatcgtcgt
      781 cctgcatcga gcggctacct gcagagataa actacctaac acgagagtaa gtagtctgtc
      841 tgtagtcgtc cagtctgatg atttttacga tacttcgttt ttctgatcat acagttgcct
      901 ctcggcatag gtcagaactt taccttgtct gggtggtgcc cgttggtcgt agatagattg
      961 gatatcgcta ggagaatcct tttgaactgg aggaaagagg gcatcaatca agctactgaa
     1021 tactttaact aagtcaaata tagtattttt cttagtagac ctaacccttt tttcataatt
     1081 tctaatggtg tttttactta tacctatctt agtacccaat tcttattgag tccaaccatt
     1141 actagtctat attgttttat agttgattga gtttggaata gtacgctgta gctgctaaaa
     1201 catttctagg aattaatttg cctttcctaa tagagttgtt catatcttat ttcaatctat
     1261 tatatttttc gcctctaaca atcgcaatct cttctttatc tacgaacgaa atcgctttct
     1321 atttttgtaa gtaaagcatc acacgaaatc cacgaaaatg aaaacctttg ttgtgttttc
     1381 gtaaaaaatt tgttgacaga gcacgaaacg cctactataa ttaaatcaag tttaaggaaa
     1441 taacaaaaaa acaaatcgga gggaaagacg atgaacacat taaatgagaa agtaatcaat
     1501 atctgtaaag cagtagttaa agaaacttta atccaagaca tttaggagaa agaaatgaag
     1561 aaatcaagaa aattagccac attaggaatc tgttcagcct tgtttttagg cttggctgct
     1621 tgtcaacaac aacatgctac ttctgagggt acgaatcaaa ggcaaagcag ttcagcgaaa
     1681 gttccatgga aagcttcata caccaaccta aacaaccagg taagtacaga agaggtcaaa
     1741 tctctcttat cagctcactt ggatccaaat agtgttgatg cattttttaa tctcgttaat
     1801 gactataata ccattgtcgg ctcaactggc ttatcaggag atttcacttc ctttactcac
     1861 accgaatacg atgttgagaa aatcagtcat ctctggaatc aaaagaaggg cgatttcgtt
     1921 gggaccaact gccgtatcaa tagttattgt cttttgaaaa attcagtcac cattccaaag
     1981 cttgaaaaga atgaccagtt gcttttccta gataatgatg cgattgataa aggaaaggtc
     2041 tttgattcac aagataagga agagtttgat attctatttt cgagagttcc aactgaggca
     2101 actacagatg tcaaggttca cgctgaaaag atggaaacat tcttctcaca atttcaattc
     2161 aatgaaaaag ctcgaatgct gtctgtagtc ttgcacgaca atctggatgg cgagtatctg
     2221 tttgtaggcc acgttggggt cttagtacct gctgatgacg gtttcttatt tgtagagaaa
     2281 ttgactttcg aagagcccta ccaagcgatt aaatttgcta gtaaggaaga ttgctacaag
     2341 tatttgggca ccaagtatgc ggattataca ggcgagggac tggctaagcc ttttatcatg
     2401 gataatgata agtgggttaa actttaatag tgttatgata tgacagtaat cttaggattt
     2461 ttatttctat ttttgataag ttggtgggtt atttcaatcg ttaatagcca gaaaccacgc
     2521 aaaaatgaaa catttattgg ctatgtccaa agatacgata ttgatgggaa tggctatcaa
     2581 tcattaccgt gtcctcagaa ctgagaggag ttcttgaaat cgtaacacca ctttgaacaa
     2641 gagttacttc aacccattgg ctccgacgga gtaagttgct ttcgtggata ccaaaaacag
     2701 ccgcaatttg ttcataagtg cgatattctc gcacttattg aagagtggcc ataagaaggt
     2761 cttctaggct taatttaggt tttcgtccac cttttgcgtg tttaagttga taagctgttt
     2821 ttaatacagc taacatctct ttaaaagtcg tgcgttgaac accaacaaga cgcttaaatc
     2881 gtgcatcggt tagttgttta cttgcttcat aattcataga actattatac catattttgt
     2941 ttcgcaggaa gtctaatgct aaaatcttat cagtgagaag atttcttaaa gtttattctt
     3001 tgtagtttca tctgtattag tacataattt aacttagaga aaataggagg actaatcgaa
     3061 tggaattaaa agattttaca gaaaaagaac aggaaatgat taagaaaagg cttacaatgt
     3121 ctaatattag tgacaaagaa actactgaga agattctggc gctagtccca caagacttga
     3181 ttaagcgaat cccgtttttt gtcagaaaac atgctacaac acgtacgatt aaacgcattt
     3241 caattgaata ccctgaactc tacgctgtag ctcaaacaag tggtgagatt ccagaaaaaa
     3301 aacgcgaaga attgcgtcag attatcacaa ctatctttga acaaaagatg aataagcata
     3361 gtattaagta gagaatgaaa aaatatttta ttggcggttt gggaagcaat gcctatcata
     3421 gcaaggattt tcttcaagaa ctagattcgc aggtctattt tctaaatcca tatgaaaagc
     3481 atcttcgaga tgaaacagaa ttgaaatcat ggtttaaaaa tgagattgta gaggaagaat
     3541 ctatctgtct gataggtcat tctattggag gcgatttagc tcgttatttc gcatcggaat
     3601 ttgaagaagt aaagaaactg attcttttgg atggtggcta tctagattta gataagattt
     3661 tacctttgga tacagagtta gaggaaacta aaaattatat caaatctcaa attatttcgg
     3721 acttagatgt tcttacttct aaagaaaaat ctgaagcaaa gcattggtca gaaaatatgg
     3781 agaaagctgt aagacagtcc tatcactgga atgttgagta taatagatat gagttggcta
     3841 taaattatga aaatatagaa gcgatactcc gcctacggag aaaaatacaa gcttttaaga
     3901 gagaagtggg agataccttg tttattagtc ctcgctatcc taatgaagct acatggagag
     3961 aggaagccct aaaagaattg ccagactatt ttgatactat ttttttagaa aactttggcc
     4021 atgagcttta tactcaagca cctaaagaaa tcgctagtct aatgaatgag tggctcgctt
     4081 attttctatg agctacttgt attgctggaa atggtataag atgaaaatct acaagtcact
     4141 aattttcata taaccaaaaa acgaacaggc tctttttctg ttattcagaa gagaacttgt
     4201 tcgtttttat cttgattttc ttaaaaagct ctatatacat agggattttc tggtttatcg
     4261 actttggtaa agctgtcgat ttccaaggtt ggaaggtttt gtttgagttc tttatggtag
     4321 tgattgacca gttttccttt ggctgaaatc caagtgttgt tttcatactg ccgggtattg
     4381 cccttggtca gcaatccata gacaccagaa tctgcgatgc agtgaataat gccgaagcgg
     4441 aacagaaatt ggctattggc atgactgggg tcattataga caaagcctgt gaactggatt
     4501 gtcttaccct caaattcatc tggatagtcg tagatagcct ccatgacctc catatagttt
     4561 tcattagtga tctgaatact atcttgggat aagtatttat ccgccgccgt tcgcatttcc
     4621 ttttcatagg ctgattttga aaaataagaa ctggtatctg gtttcaaata ttggcttgtc
     4681 gtcccttcgc ttgtctgaat ggctagatcc gttccttccg atagggggaa atgataacct
     4741 ttagcagaaa cggtctgaga atccaagcta acagttggga aagttaagcc gatgacaagt
     4801 ggaatagcca gaagagaaat actcgtcatc ttggctaatc ggctgttcag atgactgtgg
     4861 gttttgactt gcttcatcca gatatacaat tgaacgatag ccaagataaa agaaagcacc
     4921 atggagatat aggccagata ggaatagtgc atgttgatgt actggtttag tttgcccgac
     4981 agatggaggt aaatagtcag ttcaaaatag ccagctaaaa ctaaaaatcg aatcataggg
     5041 gatgactcca atcaagagag aatagactaa gacgacaaga gttacaattg ttatgaagtg
     5101 actgataaat cgtgctttca agtaattttt catcatgaga atatttttga tatccagcat
     5161 tggaccaatg acgagaaagg ccagaactgg tgccaaaccg aaactcgaga gaagagaagc
     5221 acctataaag gcatccgcct cactacagag cgaaagaaga aaggctaaaa tcatcaagag
     5281 caggatggca aaaagagggg tcgcactgat agaggtcaga atccgagtcg gaacgtagac
     5341 ctgtattata gaagcaaaga ggcagccaaa taccaaataa cgccccgtat caaaaaattc
     5401 atcaatggcc tgcacaaaga cttgaaaaac tttttttgca gaactcaagt aagaaaaatc
     5461 atgctcatga caagccagac gattttcttt ctgaatcggt tcttgccaga aaaatcctag
     5521 aaaaattcct agtattacag ccacaagaat ggaacccaga gctcgtaata gggcgacatg
     5581 gaaggagttg ccaaaggcag aataggtcgc aaaaagaaca atgggattga taacaggtgc
     5641 tgtcacaaga aaaggaacgg ccgtgtaact tggaaccttt ttttccagaa aacgattgat
     5701 gatggggacg attccacatt cacaagaagg gaaaaacata ccgacgaagg tcccaaaaaa
     5761 gattctcccc caacgatttc gagggagaaa atgataaacc ttgtcaggtg tgatataaac
     5821 ttcaatcagc cctgagacaa tgcttcctat cagaacgaag ggaagggctt caatgataat
     5881 ggagagaaaa atggctccag tttgaagtac actagatggg agttgttgaa aagcagtcat
     5941 ctagtttttc ttttctgctt tggctttttt ctctttgtca tcagggaaag taacttgttt
     6001 caaatcaggg agtttagcaa actcttcaaa catcttgtcc aagtcgtctc gttttgtaaa
     6061 ttttacagcc ataggtgtac ctcgattcta aattctacct ctattatact attattttgc
     6121 ggaaaaggga gttttttaat ttgttttcga tagttgatac tcttcgaaaa tctcttcaaa
     6181 ccacgtcagc ttcgccttac cgtagta
//
LOCUS       DAVBNO010000084         5088 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVBNO010000084 DAVBNO010000000
VERSION     DAVBNO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.084"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(1..580)
                     /locus_tag="VQ846_001954"
                     /pseudo
     CDS             complement(1..580)
                     /locus_tag="VQ846_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(960..1820)
                     /locus_tag="VQ846_001955"
     CDS             complement(960..1820)
                     /locus_tag="VQ846_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET3076478.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            complement(1836..3113)
                     /locus_tag="VQ846_001956"
     CDS             complement(1836..3113)
                     /locus_tag="VQ846_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET3076479.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            complement(3106..3657)
                     /locus_tag="VQ846_001957"
     CDS             complement(3106..3657)
                     /locus_tag="VQ846_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3076480.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            complement(3673..4932)
                     /locus_tag="VQ846_001958"
     CDS             complement(3673..4932)
                     /locus_tag="VQ846_001958"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET3076481.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
BASE COUNT         1561 a         1157 c          821 g         1549 t
ORIGIN      
        1 tatattttca tgagcccttg ataaccactg tcagccaaga ttttaccagc ttgtccgata
       61 tttctgcgac tcattttgaa caacttcata tcatgacaat agttcacagt gatatccaaa
      121 gaaacaattc ttccttgact tgtgacaatc gcttgagcct tcatagcgtg aaatttcttt
      181 ttaccagaat aattggctag ttgatttttt tagggcgatt ggtttttact tccgtcgcat
      241 caatcattac cgtgtcctca gaactgagag gagttcttga aatcgtaaaa ccactttgaa
      301 caagagttac ttcaacccat tggctccgac ggattaagtt gctttcgtga ataccaagat
      361 cagccgcaat ttgttcataa gttcgatatt ctcgcacata ttgaagagtg gccataagaa
      421 ggtcttctag gcttaattta ggttttcttc caccttttgc atgtttaagt tgataagctg
      481 tttttaatac agctaacatc tcttcaaaag tggtacgctg aacatcaaca agacgcttaa
      541 atcgtgcatc agttagttgt ttacttgctt catcattcat agaactacta taccatgttt
      601 tgtttcgcag gaagtataat ataaaaaaat aaatttaaga ttggcaatac gaacgttaaa
      661 cagaattcca tttctctttt tagaatcaaa acgacaatat tgtggaatct ctacaggaac
      721 aatagactgc gacactaaaa tatcctccct tagttttact ttactaatag tatataggag
      781 gagagccctg aatcctagac cctttgttat gatgcgctta caaacagagt tggaaaaaat
      841 cttaaaagca gaaaaacgca tgatatcagc ccctaacact tgatacaatg cgttttatta
      901 tgaaaagatc tactggattt tttatcaaaa taaacttcca gtagtctttt cactttttat
      961 taataagttc cttcttcacc ttggctagtc aagataacag gtccatcttt cgtaatgaca
     1021 aattggtgtt catactgaca tgacaatcca ccgtcaatgg tcttatgcgc ccaaccagtt
     1081 ttcatatctg tatcaatttc ccaatcgcct gtattgatca ttggttcaat ggttaagacc
     1141 attccttcac gaagacggag tccacgacct gcaataccat agttaggaac cattggttct
     1201 tcgtgcatag ttgggccaac accatgacca accaaatcac gcactacacc gtaaccacga
     1261 ctttcagcgt attcttgaat agccgcaccg atatcaccga tacgatttcc aacaacagct
     1321 tgctcaatac ccttgtacat agcttctttg gttacatcca tcaagttttt gacttcttcg
     1381 gacggtgtac caacagcata agcccaacat gagtctgcta aaccaccaga atagctctga
     1441 gtatattttt tcatttgttc aacattgttg aagtttaatt ttgagacatt caggtcagat
     1501 ttagcaatgg gacctcccaa aaccatatca actttgagca aatcaccatc tttcaagata
     1561 taatgacgag ggaaagcgtg agccacttca tcgttaagag agcaacaggt agcataagga
     1621 tagtccatca tggcaccgtc aaccccaatc tgaagtggaa ggaaattttc ttctttacaa
     1681 cgacggcgga catattcttc aacttcccac atatctacgc ctggcttaat caaatcacgt
     1741 aagcctatat gaatacttgc tagaaaatca ccagccttgt ccatagcttc gatttcacga
     1801 gctgatttta atgttatcat cttttctcct agtttctaat taattttaac agtcacattt
     1861 gcttttgaaa caatctggtg accatgataa atatcgtaat caattatagc tgaccgtctc
     1921 gtatgatgaa taatccgtgc ctgaatgcgc aatatatcat ctatctgaac agcctgcaaa
     1981 aagtagatca gcatctgctc gataatgaga ttgcgaccac tattaacaac taaatcttgg
     2041 gtcatgtggg tcagaatttc tgccaataca ccattagcca aaactccatt tttttctagc
     2101 ataaagggtt ccactgtaat gactacttca tcatggtgat aagagagctt ttgtccaatc
     2161 tgctcagaaa aagttggtag agccgaaact tgggaacggc tcatcttctc catgacatct
     2221 cgtcgcgtca caacgccaag caaagtttga ttgcttcgaa caactggtac catttcaaag
     2281 tcttctgcga tcatccgttg actcacattg gcaatatttg tcgataatcc aaccaaaaat
     2341 agactacgag acataacctt atcaattgtc gtgcttggtg atttatcacc agcgtctctc
     2401 atggttacaa cgccaacaac gacctgatgt tgattgataa cagggaaacg gctgctacga
     2461 ttcttacgaa ccaagtccaa ataatcttta actgtatctg tctctctcag aaaaccatac
     2521 tcatgactag ggcgataaag tttctcaact gtcagaatat cagtcttgat ttggacattt
     2581 gacaaggctt tattgatcat ggtcgcgacg gtaaaggtat catgcttact tcttagaaca
     2641 ggaattcctt tttgattggc cagtttaagc acatcatcat gaacctgaaa tccccctgta
     2701 accagaactg cattttcatt ttccaaggct agcaactgaa tacgggttcg gtctccgaca
     2761 atcaagagcc ccccatcatg aaggtaagac aagatatttt gttcagtcat ggcaccaatt
     2821 gaaaacttac taaattctct ctctaaacct tcttgcccag ccagaacctc agaagaagtc
     2881 acttctgcaa tttcagcaaa cgttaatctc tctatagcaa ctttctggga tttaacacga
     2941 attgttccac ttctaggacg ggtctccaca attccacggt tttcagcttc tttaatagcc
     3001 cgataggctg ttccatcact aactcctaga tgattcgaaa tgctacgaac actgaccctt
     3061 ttacctactg gtaattcctc caaatagctt agaatttcct gatgcttact cattgaactc
     3121 tccttttaca taccgaaatt caagataatc ccgcattttt cttgtgtaac gatcacttcc
     3181 cttaaactga cggaacaaac taaatccaga cttaagagca actctttggc tagcttcatt
     3241 ttcaaggtgg gtaatgatag ataattgttt taagccaaat tcctcaaaag aaagctgaca
     3301 aatttttcta acaacctctg tcataaatcc ttgcgaccaa gcatcttttc tcaaaaaata
     3361 gccaagctca gcttcttttt tgatttcatc taacttctca aatttaatag aaccaatcat
     3421 ttgttgattt ttctggtcac aaattgccca cactcccaaa ggggacttca taaagtaatt
     3481 ggccagtgca tattgacttt cttccagact tgcctgcgtt gggaaaataa attgaagatt
     3541 ttctggattt gaagctatct cgcggaagtc ctgactatca ctaaaaaaga aaggacgcaa
     3601 atacaagcga tccgtttcaa aaaaagaaaa cattgctaat ttggtccaaa tattcataaa
     3661 cacctctacg gtttaatcct caacaagtct aatatccgct cctaaattac gtaatttttc
     3721 gataatatca gaataaccac gtaagataaa ctcgatattg gtaatttcag ttttaccttc
     3781 agccataagc ccagcaatga ctagtgcagc cccagctctt aagtcggtcg ctttaacact
     3841 ggccccacgt aaatcacgtc cacccgtgta caaaatatga ccatttgttg tcgaaatatc
     3901 cgcatccatc tttgctagtt caaaaacatg atttacacgt ttttcgtaaa tcgtatcgac
     3961 aattgtacca cgaccattcg ctcttagtaa aagaggggta agcggttgtt gcaaatcagt
     4021 tgcaaagcct gggtaaggag ctgtcttaat attgattgct ttcaaattag actgttcctc
     4081 gacaaaaatg ctgtcttcag atacagtcat tctcactccc atttcttcca acttagcaat
     4141 aaacccttcc aggtgttcgt aaagaacatt atttatacga attcctttac caactgcagc
     4201 agctaaagat atatatgttc cagcttcaat gcggtctgga atcacctgat gacgtgtccc
     4261 atgtaatctt tcaacaccat caataatgat gatattagtt cctgccccac ggatatgggc
     4321 acccatatta ttcaagagag tagctacatc aataatctca ggttcacggg ctgcattttc
     4381 aataatagta cgaccatttg ctttaaccgc agcaatcatc gtattaatcg ttgctcccac
     4441 actaaccgta tccatgtaaa tacttgcacc atgaagtcct gtatctttag cagataactt
     4501 catgttatct ccctcgtagc tagcagtggc acccatagct tcaaacgcct taaggtgtaa
     4561 gtcaatcgga cgaggaccaa gatcacatcc tcccggtaga ccaactgtcg cttcaccaaa
     4621 acggcctaag aggctcccat aaaaatagta agatgcacga agactgttaa ttttaccata
     4681 aggcattgga atattttgaa cacctcttgg atcaatctcc aagacatcgt cataacgctt
     4741 aacagtagct cccatcaatt ccatgatttc gacaagactg gctacatccg aaatatctgg
     4801 aacacaatcc aaagtcacca catcatcagc caagataata gctggaatta aggccacaac
     4861 actattttta gcaccactaa tagtaatttc accttgcagt ggtaatccac cattgataac
     4921 aatttttctc attctaagtt ttcaatactc tttcaagatt tctaataagg tcttcaaaat
     4981 aaagtatatc ataaatttat ccttttttcc atccttttca attttattgg ataaatcaga
     5041 gttgacgagt tagtaactga attactaata ctcaataaaa atcaaaga
//
LOCUS       DAVBNO010000085         4594 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVBNO010000085 DAVBNO010000000
VERSION     DAVBNO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.085"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(1..170)
                     /locus_tag="VQ846_001959"
                     /pseudo
     CDS             complement(1..170)
                     /locus_tag="VQ846_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(216..449)
                     /locus_tag="VQ846_001960"
     CDS             complement(216..449)
                     /locus_tag="VQ846_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET3076482.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(455..1447)
                     /gene="plsX"
                     /locus_tag="VQ846_001961"
     CDS             complement(455..1447)
                     /gene="plsX"
                     /locus_tag="VQ846_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET3076483.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLVADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDIGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1444..2214)
                     /gene="recO"
                     /locus_tag="VQ846_001962"
     CDS             complement(1444..2214)
                     /gene="recO"
                     /locus_tag="VQ846_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET3076484.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2211..3380)
                     /locus_tag="VQ846_001963"
     CDS             complement(2211..3380)
                     /locus_tag="VQ846_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET3076485.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKVVILNYPANPTGITYSREQLETLAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQTIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3529..4539
                     /locus_tag="VQ846_001964"
     CDS             3529..4539
                     /locus_tag="VQ846_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET3076486.1"
                     /translation="MSFLSKNGAGILVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNALESATIVKLT
                     RTLAIIPITIFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
BASE COUNT         1199 a         1181 c          868 g         1346 t
ORIGIN      
        1 ttctcaacct tgcttctctc cttagatagc gcatgattac aggctttatc ttcaactgtt
       61 agcggcttga gtttgctgga tttacgtgga gtttgtgctt gaggatatat cttcatgagc
      121 ccttgataac cactgtcagc caagatttta ccagcttgtc cgatatttct catttctaaa
      181 aaccatctac ttccgttgac ttggaactcc gattgctatt ttccttgaat gattttaacc
      241 acatctccta cgttttggag ttggtcaatt tcttcatcgc tgatttcgat actaaattca
      301 tcttccagag tcaagataaa ctccatcaag tcaacagaat ccgcatccaa atcgtctttc
      361 agactcaagg attctgtcac gacaaagtcc tctccctgtc gctcttggat aatggtcaca
      421 atacggtcaa aaatttcttt ttctgtcatc tcttttattc tcctgaaaat tcacgcgcag
      481 tctgggcaac cacgtctgtt tctagcatgg tacggatctg acgaatcgta ctataaacag
      541 ccttggcatc gcttgagcca tgagtcttga caacaggtgc cttaacacca aacaagaccg
      601 ctccaccaat atctgaataa ttgagttgtt ttttcaaacc tctgaggctg tccttgagaa
      661 ggagggcacc tagtttcgct cgaagaccac cacctgtaat agctgtcttg agcaagccca
      721 tgattcccat agctgtccct tcgatggatt tgagcacagc gtttcccgtg aaaccatctg
      781 ccacaacaac atctgcaacg ccattcatca aatcacgcgc ttccacattt ccgataaagt
      841 tcaaactttc atcagccact agtaattcat aagtttcctt acgaagcgga tcgcccttgc
      901 tactctctgt tccgttattg agcaagccaa cacgtggttg cgcaatgcca cgaacatttt
      961 tagcatagaa ggatcctaga accgcgtatt gatggaggtg ctgggctgta ttttccgcat
     1021 tggcaccgag gtctagcatg tcaaatccct tcccatctac agtcggcaaa gtcgacataa
     1081 gcccaggacg gtcgatattc ttgatacgac ccacgatgaa gaatccagca gccaacaagg
     1141 cacctgtatt cccagccgaa aggacagcgt ctgcttcacc atctttgaca gccttggctg
     1201 ccaataccat actggcattt ttcttattcc taatagctct cgtaggttca tcatccgaat
     1261 caatcttctc atccgtatgg ataatgctga cgcgctctgt cgctgtcaga tattgcttga
     1321 ttttagcttc atctccgtaa agttgaacct cgatatctga aaagtcagat agggcttgat
     1381 tgacaccctc aacaatggcc tgaggtgcgt aatcgccccc catggcatct actgcgattt
     1441 ttttcatttc ttttcctctt ttagtaattg tccccagtct gctagggaat caataaattt
     1501 ctttgatttt aggtgaatcc caacgtactc ttcatataat tgatccataa attggcgtag
     1561 ctcttgcttg attccaggct tgagcgaaat ggtctccaaa gtctcaaaat caatagcttg
     1621 aaattgattg agcagatagg ggatattggg attgagatga caacgtctct tatcctcatg
     1681 ataatgctct ggacagaggc aggctccata tttgaaagaa aagtcaaaag cctgaccaac
     1741 ccgatggcag aagacacact cattaaaatt gaggctgatt ccaaatcgag tcaaaatttg
     1801 aatttcaaaa atattggtca aaacctgata atccaagcct gcttccatca actccaaagt
     1861 cttttgcaaa aaagcaaaca agggagcatc ctgctgattg tcctgcaaac tagcatctgc
     1921 aagagctgcc acataggtcg cataggccat gacaaagagg tcactattaa tcttgggaaa
     1981 agtcatgacc tcatgatagt cttcgatgta actgagtccg tcatcattga ttcgcaagag
     2041 aaatcgtgcc agcaccaagg gctgaataac aggcgccagc ttagactgac cagcgtgttt
     2101 gacaaaaaac atgcgtttgc caacctgctc tgtaaaaatt ttgacgagct tgtcatcctc
     2161 acgaaaattg cgattgtaaa gcaccaagcc ttgactcgtg atagactgaa tcatgcttct
     2221 ctcatgtact cctcaagtcg tttcatggct tctttgatag tctccatgct ggctgcataa
     2281 gataggcgga cgtagccttc cccgtaacgt ccaaaggctg caccagggat aaaggcaacg
     2341 gccttcttct gagcaaaatc cttcagaaaa gcaaaggagt cttgattgta gcccgctgga
     2401 attttagcaa aaatatagaa ggcaccgtct ggtttgataa tctcaaaacc aagagcagtc
     2461 attttttcga tgatatagtc ccgacgttgg atatattcct tcttcatggg ctccgcatcg
     2521 tttttaccag ccgtcaaggc ttctaccgca gcatgttgcg ccatggtatt tgcggcagtg
     2581 accaagtact ggtgactctt gattaactgg gctgtgaagg tcgcaggagc gaaaatcagc
     2641 cccaaacgcc aacctgtcat ggcatgcgat ttagacaaac cattgataat aatagtctgg
     2701 tctctcaaca tcgttcctag agacacatgg gcttcgcctg tgtaggtcaa ttctgagtaa
     2761 acctcatcac agacaacaaa aatttcatac ttgcgtaaaa cagctgccaa ggtctctaac
     2821 tgctctcgac tgtaggtaat tcctgtcgga ttggctggat agttgagaat aaccaccttg
     2881 agcttatcac cctgctccaa aatggccttc tccaacatct caggagtcaa gacaaaacca
     2941 ttttcagtcg tatcaatctc aacaatttct gccccaacta agttaacaat cggttcatag
     3001 cctggataag caggagctgg caaaagtacc ttgtctccct cttccaaaat agccgtcaaa
     3061 gtcgcagata aagcctctgt cgccccaatt gtaaccaaga tttcattttc aggagcatag
     3121 tccagttggt acttttcctt aacaaagtca ctagctgcct gacgtagagt cagcagacca
     3181 ctcatccctg tatagtagga ttggttctga tcaatcgctc gcttggccgc ctccttgaca
     3241 tggtctggcg ttgtaaaatc aggttccccc aaggtcaaac gcaagacccc aggaatctcc
     3301 gaaatagcct ggtcaaactg acgaatcaac gaaacttgaa ttttatctaa ctgtttatta
     3361 aagcgcttag ttaagtccat agatacctcc tactttcaaa acgtatctct attatactac
     3421 aaaagcctcc cgaccgcaaa aatacattag agaatagctt tccactttct attttacttt
     3481 tcctctttac aggtctataa tggtaacaga ttctatttgg aggtttttat gtcatttcta
     3541 tcaaaaaatg gagcaggcat cttggtctgc cttctcattt ctatcgtatc ttggtactta
     3601 ggaggattct tccctgtgat tggcgcaccc gtttttgcga tttttatggg catgctccta
     3661 catccctttc tctcgtctta taaacaactg gatgctggat tgacctttag ttccaagaaa
     3721 ttgctccagt atgccgttat cttgcttggt tttggtctca atatctctca agtcttcgca
     3781 gttgggcaat cttcactccc tgtcatcctt tccaccattt caatagcctt gattgttgcc
     3841 tacctcttcc agcgcttctt tgccctggat acaaaactgg ctaccttgat tggagtaggt
     3901 tcttctatct gtgggggttc tgccattgca gcgacagcgc ctgttatcca tgctaaggaa
     3961 aaggaagtag cccaagccat ctccgttatc tttttcttca atgtcttggc tgcgctcatc
     4021 tttccaaccc tcggcacctg gcttcatcta tccaatgacg gcttcgccct ctttgcagga
     4081 actgcggtca atgatacttc ctctgtaacc gccacagcta gcgcttggga cagtctctac
     4141 cagaccaatg ccctcgaatc cgcaaccatt gtcaaactca cgcgcaccct agctatcatc
     4201 cccatcacta tctttctctc ctactggcaa agtcgcgagc aaaaaaataa gcaaggctta
     4261 caactgaaaa aagtcttccc actttttatc ctttacttca tccttgcatc gctcctaacc
     4321 acccttctga cctctctcgg tgtgtctagt agtttcttta cccctctcaa acaactctct
     4381 aaattcctca ttatcatggc catgagtgcc atcggtctca aaactaatct ggtcgctatg
     4441 gtcaaatcca gtggaaaatc cattgttctc ggagccgtct gctggattgc catcatcctc
     4501 accagccttg gtatgcagac ccttatcggc attttctaat actcttcgaa aatctcttca
     4561 aaccacgtca acgtcgcctt gccgtatata tgtt
//
LOCUS       DAVBNO010000086         4587 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVBNO010000086 DAVBNO010000000
VERSION     DAVBNO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.086"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(80..517)
                     /locus_tag="VQ846_001965"
     CDS             complement(80..517)
                     /locus_tag="VQ846_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET3076487.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLKAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(602..3235)
                     /gene="polA"
                     /locus_tag="VQ846_001966"
     CDS             complement(602..3235)
                     /gene="polA"
                     /locus_tag="VQ846_001966"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET3076488.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3491..3841
                     /locus_tag="VQ846_001967"
     CDS             3491..3841
                     /locus_tag="VQ846_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET3076489.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
     gene            4056..4160
                     /locus_tag="VQ846_001968"
     CDS             4056..4160
                     /locus_tag="VQ846_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET3076490.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGG"
     gene            4165..4398
                     /locus_tag="VQ846_001969"
     CDS             4165..4398
                     /locus_tag="VQ846_001969"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076491.1"
                     /translation="MFSQLRMREEQALLAQDYALETARAGGIEQGLERGKVEGREERK
                     LFAFLDIVRQDLLTSEVASQQLGMSVSEFEALL"
BASE COUNT         1399 a         1091 c          846 g         1251 t
ORIGIN      
        1 gcccgcggct agtttcctag tttgctcttt gattttcatt gagtataaca caaaaggtag
       61 cccatcagct acctttttct tatgcttcct caatcaagcg agtatgttct ctcttgatac
      121 agcgattcat cacgatatca tcacatccac catcacgcaa aatctctttc gcttttaaac
      181 tttcaagtcc tagctgtgcc caaaaaatct tggcatcagc tttgagaaaa tcacgcgcca
      241 catcgggcag aaattcactg cgacgataaa cattgacaat atctacagga aaaggaattt
      301 cagcgaggct agcataagcc ttttcaccca agatttcgcc acctgccgcc ttgggattga
      361 ctgggatgat tttatagccc cgagcctgca tttcctttgt cactcgattg ctggttgttt
      421 cttcacggtc agacaaaccc accacagcaa gggttttact cgttgcgaga tactgacgaa
      481 tcacgccatc acttggattg ataaattctt gactcataga aaacctcctt tttcatcagt
      541 atagcacatt ttgaaaaggt ttgcagaatt ctactacaaa aaaggaggac tagccccctt
      601 tttatttagc ctcgtaccag gttgcccctt cattctcatc tgcgataaga ggaacactga
      661 gttgaatggc ttcttccatg gtttgtttca ccaatttttt catctctacc aattcagatt
      721 taggcacttc aaggacgatt tcatcgtgca cttgtaacag catcttagtc tgataaccac
      781 ctgcaaccaa ggctttatcc agctgaatca tggcaatctt gagaatatct gctgccgaac
      841 cctggatagg tgagttgata gcagttcgct ccgcaaaacc acgaatattg aagttgcgcg
      901 aattgatatc tggcaactca cggcgacgct taaagagggt ctctacatag cccttatcac
      961 gcgcctcccg caccacttca tccatgtagt ttttaatacc tggaaaacgt tcaaagtagg
     1021 tatcaatgta ggctttggct tccttacgac taattcccaa attattagac aagccaaagt
     1081 ctgaaatccc ataaaccact ccaaagttaa ctgccttggc attgcgacga tcatttgcag
     1141 tcacattatc aggacgctca atgccaaaga cccgcatggc tgtcgaagta tggatatctg
     1201 ccccctcttg gaaggcctta atcaagtgct catccttaga aatatgcgcc aaaacgcgca
     1261 attcaatctg tgaatagtca gagctgagta gcacactatc ctcccactct ggcacaaaag
     1321 ccttccgaat caagcgccct tgttccaatc gggcaggaat attttgcaag tttggatcca
     1381 cactagacaa acgcccggtc tgggtcaaat cctgcatata gcgagtatga atctttccat
     1441 cagccaaaat ccagtcctgc aagccaatta cataagtaga ttgaatctta gcaatttgac
     1501 ggtaatccag gattttctta acaatcggag caataggagc gagacgctcc aacacatcca
     1561 ctgctgtcga ataacctgtc ttggttttct tagtgtattc tagaggaagt cccaatttct
     1621 caaagagaag cacgcccaac tgcttaggcg agttgacatt aaactcctca ccagccagct
     1681 cgtaaatctc ttgagtcagt ttttcaatga caagctcatt ttcagcctgc atctcaagca
     1741 aggtctcttt cttgaccata atcccagcaa tttccatctt ggcaaggaca aaagccagag
     1801 gttgctccat atcataaaga agctctaatt gcccattttc gctgagtttt tcaagtaaaa
     1861 taggctctgt ttctaccaaa acagcaagtt tacaagctaa gtgttccaag aatttctcac
     1921 gttcaggaat ggccttttta acacccttac cgtagaaagt ttcatcatca accaagtaag
     1981 tctgaccata aagactagcg atggtcgcaa tttcattgtc ctccacagtc gaaaggaggt
     2041 atttagccaa acggatgtca aaagcaggcg cctgcaaatc cacaccaaaa cgttgcaaaa
     2101 gaactttaac cttcttaaag tcataaactc tcagagatgt tttttctaag aaatccttga
     2161 aaatcgggtc ttgcaacagc tcaagcttgt ctgtggcata gagcttatcc ccacaagacc
     2221 aggcaaatcc aaccaaatta tccgtatggt aattctcacc aaaaagctca aagtggaaga
     2281 tagactcttc actcagcata tcttgactga tttggtcaac aatagtaaaa tccaaactct
     2341 cagacacatc agctgacgac acatttaaag cctgctttag ctgtttgaag cccatctcat
     2401 cgtagaattt cccaagattt tcaacatctg gaccactata gaccaagtcc tctaaaccaa
     2461 tcgcaatcgg tgccttggta tcaatggtcg ctagtgtttt agataaaaag gcctgttcct
     2521 tgtcattgat gagattttcc ttcatcttag aagtcttcat tccatcaata ttttcataaa
     2581 tcccctcaag cgaaccatgc tccagcaaga gcttaatacc cgtcttttca ccgactttgg
     2641 tcaccccagg gatattatcc gacttatcac ccatgagcgc cttgagatcg ataaactgag
     2701 ctggtgtgag gcccatttct tccatgaggt aatctggcgt aaaggcctca aactcagcca
     2761 cacctttctt ggaaatttca accaccgtat gctcatccgt cagctgaatc aaatccttgt
     2821 ccccactgac aatggtaata tcaaaaccat cctgctctgc tagcttatcc agcgtcccaa
     2881 tgatgtcatc cgcctcatac tgagccagct catagtgacg aatccccata tgatccagca
     2941 actcacgaat gaaaggaaat tgctcacgaa actcatcagg agtcttggcc cgaccaccct
     3001 tatagtccgc atacatctct gtccggaagg tcgtctttcc cgcatcaaaa gccaccaaaa
     3061 tatgactcgg ctcaacccgc tccaacaaat gactcaacat caactgaaaa ccataaatcg
     3121 cattggtatg caaaccagcc gcattcttaa aacggtccaa ctgctgatac agcgcaaaaa
     3181 acgcccgaaa agctacagaa gacccatcaa tcaataataa ttttttctta tccatacacc
     3241 cattataaag gaaagaatca aaaaatacca ttgggaagag ctagagcaag tatttttcaa
     3301 actttttccg aataaataga tagagccaga gaatttagta aacctagatt taaaaatgtg
     3361 ctataatata gtatattgaa tctataatag tacaccttga ctgctaaaat atttctataa
     3421 attaatttga ctttcctgat agagttattc acatcttatt tcaactcact atagaaggag
     3481 gaataggagg attctcagac atccgggcat cagcccaact aatgatttga ttgctaagaa
     3541 aatattcagc aatccagaaa tcacttgtca atttattcgc gatatgctgg acttgccagc
     3601 aaaaaatgtg accattttgg agggaagcga tattcacgta ttactctcca tgccttactc
     3661 ggtgcaggat ttttatacca gtatagacgt cttggcggag ttggataacg gtactcaagt
     3721 aattattgag attcaagtcc atcatcagaa ttttttcatc aatcacttgt gggcttacct
     3781 gtgcagtcag gttaatcaaa atcttgaaaa aattcgtcag cgagaaggtg atactcacta
     3841 gatctacaaa cacatcgctc ctgtttacgt cattgctatc gtggatagta attatttctc
     3901 agatgacctg gcttttcata gctttagtat gcgcgaagac acaacaggtg aggtattggc
     3961 gattaccaac aatggacagg aaaaccatct ggttaagatg gcattcttgg aattaaaaaa
     4021 tacagagaaa ccagcaaaga caaggttcgc aagccatggt tggagttttt cggcaacaag
     4081 ccctttaccc agcaaccgca acgagccatt acccaagcaa atcaactgct ggactacaag
     4141 agctggtccg aggaggatag gaaaatgttt agtcaactac gtatgcgaga agaacaagcc
     4201 ttgttagcgc aggactatgc cttggaaact gctagggcgg gaggtattga acaaggctta
     4261 gagcgtggga aagttgaagg aagggaagaa aggaaacttt ttgccttcct agacatagta
     4321 cgccaagatc ttctgacttc tgaggttgcc agccagcaat taggtatgtc agtatctgaa
     4381 tttgaggcac tgttgtaaaa tggctccata atatccatag tgggtaaatc ccctatggat
     4441 attatggagc ctattttgtg tagaaaaaaa gtcccatatg acctataatg aaaagcgaca
     4501 aaacaactca ttagaaagaa tcatatggaa caattacatt ttatcacaaa attactagac
     4561 attaaagacc ctaatgtcca gatttta
//
LOCUS       DAVBNO010000087         4526 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVBNO010000087 DAVBNO010000000
VERSION     DAVBNO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.087"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..278
                     /locus_tag="VQ846_001970"
     CDS             <1..278
                     /locus_tag="VQ846_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET3076492.1"
                     /translation="GSWYYLNANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWY
                     YLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVDGYEVNANGEWV"
     gene            679..1800
                     /gene="mnmA"
                     /locus_tag="VQ846_001971"
     CDS             679..1800
                     /gene="mnmA"
                     /locus_tag="VQ846_001971"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET3076493.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1941..2396
                     /locus_tag="VQ846_001972"
     CDS             1941..2396
                     /locus_tag="VQ846_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET3076494.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALILQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2406..4319
                     /gene="mnmG"
                     /locus_tag="VQ846_001973"
     CDS             2406..4319
                     /gene="mnmG"
                     /locus_tag="VQ846_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET3076495.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
BASE COUNT         1306 a          918 c         1105 g         1197 t
ORIGIN      
        1 acggttcatg gtactaccta aacgctaacg gtgctatggc aacaggttgg gctaaagtca
       61 acggttcatg gtactacctc aacgctaacg gttcaatggc aacaggttgg gtgaaagatg
      121 gagatacctg gtactatctt gaagcatcag gtgctatgaa agcaagccaa tggttcaaag
      181 tatcagataa atggtactat gtcaatggct taggtgccct tgcagtcaac acaactgtag
      241 atggctatga agtcaatgcc aatggtgaat gggtttaagc cgattaaatt aaatcatgtt
      301 aagaacattt gacattttaa ttttgaaaca aagataaggt tcgattgaat aaatttatgt
      361 tcgtattctt taggtaccta tcttatgatt tcaggaaatg tcattaaaaa aacgactcct
      421 tttctctaac ttgaaaaata gattagagaa aatgggttgt tttatttatt atagttattt
      481 gaatgaagat aagaagaagg tatactcaca tcattcacat aatctgtata ttgactataa
      541 gttttaaaaa acaattttta agctcttcct tgtcttctct aaccaagcgt gttataatga
      601 atactgctca agcgaccttc aatcgtgaag cacacacgac cttcaatcgt gaataaacga
      661 atagatggga gacttaccat gagtgataac tctaaaacac gtgttgtcgt ggggatgagt
      721 ggtggtgttg attcgtcggt gacggctctt ttgctcaagg agcagggcta cgatgtgatc
      781 ggtatcttca tgaagaactg ggatgacaca gatgaaaacg gcgtctgtac ggcgaccgaa
      841 gattacaagg atgtggttgc ggtggcagac cagattggca ttccctacta ctctgtcaat
      901 tttgaaaaag agtactggga ccgcgttttt gagtatttcc tagcggaata ccgtgcaggg
      961 cgcacgccaa atccggacgt tatgtgcaac aaggaaatca agttcaaggc ctttttggac
     1021 tatgccatga ccttgggggc agactatgta gcgactgggc attatgctcg agtggcgcgt
     1081 gatgaggacg gcactgttca catgcttcgt ggcgtggaca atggcaagga ccagacctat
     1141 ttcctcagcc aactttcgca agaacaactt caaaaaacca tgttcccact aggacatttg
     1201 gaaaagcctg aagttcgaaa actagcagaa gaagcaggcc tttcgactgc taagaagaaa
     1261 gactcgacag ggatttgctt tatcggagaa aagaacttta aaaacttcct cagcaactac
     1321 ctgccagctc agcctggtcg catgatgact gtggatggtc gcgatatggg cgagcatgca
     1381 ggtctgatgt attatacaat cggtcagcgt ggcggactcg gtatcggtgg gcaacacggc
     1441 ggtgacaatg ccccttggtt cgttgtcgga aaagatctaa gcaagaatat tctctatgta
     1501 ggacaaggat tctaccatga ttcgctcatg tcaactagcc tagaagccag tcaagtccac
     1561 tttactcgtg aaatgccaga agagtttacg ctagaatgta cggctaaatt ccgttaccgt
     1621 cagcctgact ctaaggtgac cgtccatgtc aaaggagaca aggcagaggt catctttgcg
     1681 gaaccacaac gcgcgattac accaggccag gcagttgtct tttacgatgg cgaagagtgt
     1741 ctaggtggtg gtttgattga caatgcttac cgcgatggac aagtttgtca gtacatttag
     1801 attgacaaat tttctcaatt tgctacaata ataacagcaa tagaaatgat ggtcaaagct
     1861 catggatgtt gcaggctttt ttgtcctgca cttctttgga gttttgactg tttttgtgtc
     1921 gtttaaggga aaggacaaga atgactcaac aagactttcg gacaaaagta gacaatacgg
     1981 tttttggtgt tcgggcgaca gctttgattc ttcaaaatca caagcttcta gtcaccaaag
     2041 acaagggcaa gtattacact atcggcggtg cgattcaagt caatgaaagc acggaagacg
     2101 cggtagtccg tgaagtgaag gaagaactgg gtgtcaaagc tcaagctggg cagctagctt
     2161 ttgtggttga aaatcgtttt gaacaggacg gtgtttccta tcacaatatc gagtttcatt
     2221 atctggtgga cttacttgag gatgccccat tgaccatgca ggaagatgag aaaaggcagc
     2281 cctgtgagtg gattgacttg gataagcttg aggatatcca gctagttcca gcctttttaa
     2341 aaacggccct accagattgg gaaggccaac taagacacat tcatcttgag gaataggaga
     2401 gaaacatgac ttatcatttt actgaagaat acgatattat tgtaattggt gcgggacacg
     2461 ctggggttga ggcttccttg gccgctagcc gtatgggctg taaggtcctg cttgcgacca
     2521 tcaatattga aatgctggct ttcatgcctt gtaatccctc tatcggtggt tctgctaagg
     2581 ggattgtcgt acgtgaagtc gatgccctcg gcggcgagat ggccaagacc attgacaaga
     2641 cttacatcca gatgaagatg ctcaacacag ggaagggccc agccgttcgt gcccttcgtg
     2701 cgcaggctga taaggaactt tactctaagg aaatgcgcaa gacagttgaa aatcaagaaa
     2761 atctgaccct tcgtcaaacc atgattgatg agattttggt ggaagatggc aaggttgtcg
     2821 gtgtgcgtac agccacccat caagaatatg ctgctaaggc tgttattgtg acgacaggga
     2881 ctgctctccg tggggaaatt atcatcggag acctcaagta ctcatcaggt cctaaccaca
     2941 gcttggcttc tattaaccta gctgacaatc tcaaggaact gggtctcgaa atcggtcgtt
     3001 tcaagacagg aacccctcca cgtgtcaagg cttcttctat caattacgat gtgacggaaa
     3061 ttcagccagg agacgaagtg cctaatcatt tctcatacac ttcacgtgat gaggattatg
     3121 tcaaagatca agtgccatgc tggttgacct ataccaatgg taccagtcat gagattatcc
     3181 aaaacaacct ccaccgtgcg cctatgttta caggtgtggt caagggagtg gggcctcgtt
     3241 actgtccgtc gattgaagac aagattgtgc gctttgcgga caaggaacgt caccaactct
     3301 ttcttgagcc agaagggcgc aatactgagg aagtctatgt tcaaggactt tcaaccagtc
     3361 tgcctgagga tgtccagcgt gacttggttc attccatcaa aggtttggaa aatgcagaga
     3421 tgatgcggac aggttatgct attgagtatg atatggtctt gcctcatcag ttgcgtgcga
     3481 ctttggaaac caagaaaatc tcaggtctct tcactgctgg tcagacaaat ggaacatcag
     3541 gttatgaaga agctgctggc caagggatta tcgcgggtat caatgcggct ctgaaaatcc
     3601 aaggtaaacc tgagttgatt ctaaaacgaa gtgacggtta tatcggggtg atgatcgacg
     3661 acttggtgac caagggaacc attgaacctt accgtctctt gaccagtcgt gctgaatacc
     3721 gtctcattct tcgtcatgac aatgctgata tgcgcttgac tgagatggga cgcgagattg
     3781 gccttgtgga tgatgaacgc tgggctcgtt ttgaaatcaa gaaaaatcaa tttgataatg
     3841 agatgaaacg cctagacagt atcaaactca agccagtcaa ggaaaccaat gctaaggttg
     3901 aggaaatggg cttcaagccg ttgacagatg cggtgacagc caaagaattc cttcgccgtc
     3961 cagaagtttc ttaccaagat gtggtggcct tcatcggacc agctgcagaa gacttggatg
     4021 acaagattat cgaattgatt gaaacagaaa tcaagtacga aggctatatt tcaaaagcca
     4081 tggatcaggt tgccaagatg aaacgtatgg aagaaaaacg cattccagcc aatattgact
     4141 gggatgacat cgattctatt gcgacggaag ctcgtcagaa gttcaaactc atcaatccag
     4201 aaaccatcgg ccaagccagc cgtatttcgg gagtaaaccc agcagatatt tctattttga
     4261 tggtgtatct ggaaggtaaa aatcgtagta tttctaaaac tcttcaaaaa tcaaaatgat
     4321 acgtcgtcgg cttcttacga atgagttcaa agcttggctt tgattcatct ccagcctccc
     4381 atagttcccc gaactatggg agctaactca agttatgcat tttcctagcc ttctggtctg
     4441 attttcattg agttttaatc tcaaaagaaa gcatagagaa aaacagctcc gaagacggag
     4501 ctgttttgtt gacttgtact caatga
//
LOCUS       DAVBNO010000088         4473 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVBNO010000088 DAVBNO010000000
VERSION     DAVBNO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.088"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(1..371)
                     /locus_tag="VQ846_001974"
                     /pseudo
     CDS             complement(1..371)
                     /locus_tag="VQ846_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(447..881)
                     /locus_tag="VQ846_001975"
     CDS             complement(447..881)
                     /locus_tag="VQ846_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3076496.1"
                     /translation="MLLVLVSLVHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSHLTYYWTF
                     LSGKAENQAITLYHHDQRRSGLVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(957..1807)
                     /gene="rfbD"
                     /locus_tag="VQ846_001976"
                     /pseudo
     CDS             complement(957..1807)
                     /gene="rfbD"
                     /locus_tag="VQ846_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
     gene            complement(1873..2922)
                     /gene="rfbB"
                     /locus_tag="VQ846_001977"
     CDS             complement(1873..2922)
                     /gene="rfbB"
                     /locus_tag="VQ846_001977"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET3076497.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLVAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGIYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(2932..3528)
                     /locus_tag="VQ846_001978"
     CDS             complement(2932..3528)
                     /locus_tag="VQ846_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET3076498.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     VLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENLEEADVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(3529..4386)
                     /gene="rfbA"
                     /locus_tag="VQ846_001979"
     CDS             complement(3529..4386)
                     /gene="rfbA"
                     /locus_tag="VQ846_001979"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET3076499.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSTDGLAIGEEFISDDSVALIL
                     GDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYP
                     RSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKVYLDRGDLSVELMGRGFAWL
                     NTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKNEYGQ
                     YLLRLIGEA"
BASE COUNT         1245 a         1043 c          818 g         1367 t
ORIGIN      
        1 ttggaaagga ccagatgtcc gtctttcaaa atagtcttaa aggtttcttc atacttgagg
       61 ctgtattcaa ttgcccttcc tagttttgaa cctgctaaaa ctgactgacg ccggcaccaa
      121 gcaaagaagt cttccattag gggctggaga tgttcttgac gtttctgtag tcgttcatca
      181 gctggcaaag cctcccagtc tctttccaag gaaaataact gatcacaata agctaaacct
      241 ttagctccta aggatgattt atccgtttgc ttggggggcg cttcaaaaaa cttccttctc
      301 acatgtgccc aacatccaac aagttcagct tcttccagtt gacgataagc ttctagcggt
      361 tcgcagttta ccaagcttag cgtcgccttg ctcctaaacc ttggactgct atcgcatagg
      421 cagaactaga ggactaaagt cctaagttac tgccgcaaca tatcacaatg cacatagcca
      481 gaataatctc ctaggaattc ttgtactact aaaccactcc gacgctgatc atggtggtac
      541 agcgtgattg cttgattctc agctttccca gacaaaaaag tccaatagta ggtcagatgg
      601 ctatcactct ctagaacccg ataagaggtt tcatccgcat gaagaagagg ttgttctaac
      661 aacttttctc gtaaaaggtt ataaagggac tctaaatagt attgacttgc cttgatatgc
      721 caattagaga tttccttacg tgtgattggt aaacccatcc tagcccaatc ttcttcttgg
      781 cgataattgg gtaccttcag attaaacttc tgatggatgg tgtgagcgat aatagaaact
      841 gagccaaagc tatgcaccaa agagaccagc accaacaaca agataattgt acatcttaag
      901 ctcctttgga gttcattatc tgcctctttt attagactag aaaatcattc tactacttat
      961 ctcacttctt gtttgtaaaa ttcttgcaat gcatcttgcc aagttggaat aacaaatcca
     1021 gtagctttgg ctttggccag gctcatcgtt gagtttagcg gacgttgagc tttggctgga
     1081 aattgactgg atctactggc ttgacttcga catctgtatc tttcaaaatt tcaactgcaa
     1141 aatcatacca tgttgtgtct tctgtcgcat catttgacaa atgataataa ccaaattcct
     1201 tacgattttc agctaggtag atcatgaatt cagccaaggt acgagtccaa gtcggacgac
     1261 cgtactggtc atttacaact gttaaagtct tatgagtttt cgcaagattt tgcatggtaa
     1321 aaacgaagtt tttgccataa tttccaaata cccaggcagt acggataata tagaaattag
     1381 acacatgctt ctcaactaac tcttccccca tacgcttagt gcgtccatat tctgtctgtg
     1441 gatctggtcg gtcatcaact tcccactctt gtccaactgg tttcttaccg tcaaagacct
     1501 agtccgtaga aatataaact agagttgcac catgcttttc agatgctttt gcgacatttt
     1561 ttgtccccat cacattgatg gcgaagtcca actcttttcc ttcatcctct gctgcatcaa
     1621 cagcggtgta ggctgcacaa tggtagacta aagtcggttt cacctcttca aaaactttct
     1681 caaccatttc ttcattggta atgtccatct cagccacatc tactgccacg tattcttcat
     1741 tacgttcatc caataaatag cgaagttccg ttcctaattg gccatttgcc cctgtaatta
     1801 aaatcatctc tagacctttc taatatgtaa ctcttcccaa tatagcaata aaaagcagcg
     1861 atttcctgct ttttatactg taataatctc ctgagtctta gcataattgg cttcaacagc
     1921 ttctttttct gctttccacc attcttggtt atctgtatac cacttgattg ttgccttgag
     1981 cccagcttca aagttggtaa attcaggttt ccaccccaac tcatcacgga gcttgctggc
     2041 atcaatcgca tagcgaaggt catgtcctgc acggtcagtc acatgatcat aggcatccgt
     2101 agcttgtccc atttccttaa ggataagttc caaaacttcc ttattgttct tctcaccatc
     2161 agccccaatc aagtaggttt caccgatttg cccttttgtc aagattgtcc aaactcctga
     2221 agaatggtca ttggtatgaa tccagtcacg aacgttctta ccttcaccgt aaagttttgg
     2281 cttaattcca cttaggatgt tagtaatctg acgtgggatg aatttttcga tatgttgata
     2341 aggaccgtag ttatttgaac agttggaaat cgttgccttg actccaaaag aacgcaccca
     2401 ggctttgaca atcaagtctg atgcggcctt ggttgatgag tacggagagc ttgggttgta
     2461 ttttgtttca gcagtaaatt tctcacctgg tccttcacca tgacctggca aatcttctcg
     2521 taaagggaga tccccataaa cttcatctgt cgatacatgg tggaagcgaa tatcatactt
     2581 acgagcagct tctaaaagag tatagattcc aatgaagtta gtatgaataa atggcgatgg
     2641 atcattgagc gaattatcat tgtggctttc cgctgcataa tgaacgatag catctgcttg
     2701 agcaaccaac ttgtctacca actccgcatc agcaatgtca ccaacaacta actcaacacg
     2761 attgcctaaa atttcctcaa tattcgcgcg gtttccagca taagtcaact tatctaggac
     2821 tgtcacgtga acatctggaa agttctcgta aacataatgg acaaagttag aaccgataaa
     2881 gccagctcca cctgtcacga taatattttt gtattcagtc atattctttc cttattccaa
     2941 atcttctttt ttcaaaggct tcacgtcctt aagtagggga tgatgtttgt ctgcttcaga
     3001 tacgtctgct tcttccagat tttcccattc aataccaagg cttggatcag cgtagttcac
     3061 aaaggcatac ttgggtttga gttcaagagc ccagtaatca ttgaccagat agctatatga
     3121 cactgtatct gataaaactt ggaagccatt agctacgcct cgaggaacaa agattccctt
     3181 gcttgcatca attactgtct gataggtatt cccaaaggtt tcaccctcgc gtagatcaac
     3241 ccaagaaccc agaactttcc ctccatctgc tacagagatg tacttatccc aaggctctgc
     3301 gtggaggcct cgaaggacat ttttacgtga gaaggataca ttgttttgca attttccttc
     3361 tgcaaagaaa gactctggaa atccaagtgg aagcactttt tccttttgga aattttcttt
     3421 aaaccagcca cgattatctc catgaacggg gatatcaaac tccaacatgc ctggaatagc
     3481 ttcaaccttg cgtgccgcaa gcgtcttacc gaaaaaatta tctgtcatct atgcttctcc
     3541 aatcaaacgg agcagatact gtccgtattc atttttctta agtggttggg ctaaggccaa
     3601 tacatcttct cgactgatat agcccatacg gtaagcaatt tcttctaagt ttgctacctg
     3661 aacattttgc atccgttgca ctgtttcgat gtactgtgaa gcctctagta aactttcatg
     3721 agttccagta ttcagccaag caaagccacg tcccataagc tcaacagaca aatcaccacg
     3781 atctaggtaa accttgttta catctgtaat ttccagttca ccacgagggc ttggtttaat
     3841 actcttggca atttcgacaa catcgttatc atagaaatac agtcctgtca ctgcatagtt
     3901 ggagcgagga tactccggtt tttcttctat ggaaatagca ttcatgtctg tatcaaactc
     3961 aaccacacca aaacgctctg gatccttcac ttggtagcca aaaacagtcg cacctttctc
     4021 tttcttggct gctttttgaa gcattgtgct cagaccagga ccatggtaga tattgtcgcc
     4081 taaaatcaag gcaacgctat cgtcagaaat aaactcttcc ccaattgcca aaccatctgt
     4141 acttggttgc tctgcataag aaagtttgat cccaaactca gatccgtctt gaagaagctc
     4201 ttgaaatcga tgtaaatcct gtggagtgga aataatcaaa atatccctaa tcccagccaa
     4261 catcaatgtt gaaagtgggt agtaaatcat cggtttatca taaaccggca taagttgttt
     4321 tgacgcagcg cgagtcaaag gatataaacg tgtccccgaa ccacctgcta gaataatacc
     4381 tttcatagta gggtaccttt ctttgttatt ttagagtcaa ttactattaa ttgtgtactg
     4441 ctctaaaaag aaaacaatca tcccatgttt tat
//
LOCUS       DAVBNO010000089         4347 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVBNO010000089 DAVBNO010000000
VERSION     DAVBNO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.089"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(60..659)
                     /locus_tag="VQ846_001980"
     CDS             complement(60..659)
                     /locus_tag="VQ846_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3076500.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTHPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(661..1758)
                     /locus_tag="VQ846_001981"
     CDS             complement(661..1758)
                     /locus_tag="VQ846_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET3076501.1"
                     /translation="MLERLKSIHYMFWISLIFMVFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGILLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVGGLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLVYLLVILAFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVQVENHLDKASLTQDV
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(1758..2495)
                     /locus_tag="VQ846_001982"
     CDS             complement(1758..2495)
                     /locus_tag="VQ846_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3076502.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQVVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSVVLFGLTIYLQGHKENA"
     gene            complement(2497..3381)
                     /locus_tag="VQ846_001983"
     CDS             complement(2497..3381)
                     /locus_tag="VQ846_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET3076503.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(3368..3562)
                     /locus_tag="VQ846_001984"
     CDS             complement(3368..3562)
                     /locus_tag="VQ846_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076504.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLCQFIRGALEFGFM
                     IFVVFPMGMEWLKGESHDGG"
     gene            complement(3559..3765)
                     /locus_tag="VQ846_001985"
     CDS             complement(3559..3765)
                     /locus_tag="VQ846_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076505.1"
                     /translation="MGEEEMRNKMIIAMSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         1421 a          963 c          701 g         1262 t
ORIGIN      
        1 tattgttcca ttcactaccg tcaattttag cacatagtct tcatgaaaat attatatcat
       61 cataaccaac cagattcttt tgcgatatta gctgcctctg ttcgattacc agcatccagt
      121 tttgaaagaa tattggtgac atagtttcga atagttccgt ttgagaggta aagctgatct
      181 gcgatttctt ggttggataa gccccgggcg attcccttta aaacagcaat ctcttgttct
      241 gtcaacggat taggatgcgt catcaccact tccatcaatt caggcgaata ctccttgcgc
      301 ccttcgagga cggtgtgcaa ggtttgcatg aggtctgcaa tgcttctttc tttcaagaca
      361 taagcatcca ctccagcctt gaccgcacgt tcaaaatacc ctggacgctt gaaggtcgtc
      421 accacaacca cctttgtttc aagcctttct gatcgtatcc actccaagac ttcaagacct
      481 gtcttaacag gcatttctac gtcaaggatg gcgatatcta cagactcctt ttctaatagt
      541 tggattgctt cttgcccatt cttggcttga aggacagact ccacatctgg ttgaagcgtg
      601 agcaattgac acatggcatc tcgcaacata ctttgatctt ctgcgactaa tactttcatc
      661 ttagtttctc tccttataag gtagtcgaac ttgcacttcc gttggatgtt tctgactgat
      721 tacacttact tctcctgaaa atggaaaaac acgatctcgg actgtatgga gctcatctcc
      781 ttttagaaaa gcaaagccgc agccatcatc acttactgtt aaaatgagtt ccttctctgt
      841 ccgttctaat tttaagtaag ctttagacgc tttggcatgt ttgatgatat tggtcaccaa
      901 ctcaagcaaa atcatagaag ccattgactc cacatcctgg gtcaagctag ccttgtctaa
      961 gtgattctca acttgaacct caattccagc aatttctaac atctttttca cagtctctag
     1021 ttcggatgtc aaagttctag acttaagatt ttccacaatg gttcgcactt cattcatgga
     1081 atccttgctg atctggtgaa tttcttttaa ttccttttcc acctgtggat aagcctccat
     1141 ctgaaataac tgcaaggcta gatcagtttt aacactaagc atagcaaagg tatgccccag
     1201 actatcatgc aaatcttgac cgatacgatt gcgttcattt tcagcaagca atagatttat
     1261 ctgggcattt tgcttaacct gtgcttcttt caaatcctcc acaattcgaa tccgaaccaa
     1321 gccaaaagtc attaaatcga caaaagcaag aattacaagt agatagacta gaaactcaac
     1381 ttcgattctc tgaaaaatca acagttgacc cacaacaagg acttgagcaa gaagaaaagt
     1441 ccagacatgt aaagacttta aaccacctac gctgaaatga tagcttaaga gattggatag
     1501 gaaaaagaaa aaccagatat aattaacagc cacaaaggca gtattcccaa ctacataagt
     1561 cagcaggatg ccccaatata gccaagatag acgctggctc ttagttgtta aaacacccaa
     1621 atacgccact acaaatagaa tatcaatcaa taaatgccag gcagaaagcc acccagtcac
     1681 tacaggtagg atggggaaaa ccataaaaat taaactgatc caaaacatat aatgtatgct
     1741 tttcagtctt tcaagcatta agcattctcc ttatgacctt gaaggtaaat ggtcaaacca
     1801 aacaaaacta ctgaaaaaac aagtaaataa actgtggctg atagattgat gccaccctca
     1861 tttaagaagg tcttgagcaa ctccatcaac tgataggtcg ggagacactt acctactact
     1921 tgcatccagt ctggaaataa agaaatgggc atccagagtc cgcctaaaac agccaatcct
     1981 agataaagaa gattgcccac aacagacatc agctgactag ttggtaagag agtcaaggtc
     2041 aagccaagtg ctacaaaagc aatactcccc acaatcagca aaatcgcagc tccaacccat
     2101 tttactagag gcatgtctac acctcttaca aaatgtccaa ctgtaaaaac aaccagaatt
     2161 gagaccaaat aatcaaccag catacttgtt atctttgata gataatattc taccatattt
     2221 acagggctat gacgcaatgt tttctgccag ttgttcatct tatcggtatg taaaacagct
     2281 ggaaatgaga ggatagttgt cgacatcatg gaaaaggcag tcatggagat gaggtaatca
     2341 cgcataaaat tcgcgagttc acctggtgtg tcctgataga taccagaaaa aaataaatag
     2401 aaagccgtcg gcatccctac cgacaataga taatagatta attgtcgttt ggtcaataaa
     2461 aattctatct tactaagtgc tatccatcgt ttcatcttag ttatctccct tctgcgtctc
     2521 ttcaaagatt gtattcaaca aactacgatt attaacttcg atttcttgca tcctacatcc
     2581 tgcttgggct aatagttccc agaaagcatc tgcttctcga gtgactactt gtaaagaatc
     2641 ttgctttagg gtccagtttt caaccaaatt tgactgctct acgacttcct tgtaagctat
     2701 aggaaggata aagtgctttt ctatttcctc gctacgcata gctagaggcg tcgtatctcg
     2761 aatcaactct cccttattta agagcaaaat gcggtctgca gtgtgttcca cttcctcaat
     2821 ataatgggac gaatagagaa tggtgactcc ttgcgctttt aactcctgga caatttccca
     2881 aaaacgttga cgagttgagg tatccatgga cgcagtaggc tcatctaaaa aaacaatctt
     2941 tggtcgccca attaaggtca agacgaaaga aaagagacgc ttttgaccgc ctgataattt
     3001 ttctgccaac tgttcttttt gttgcttgac aaactgcaat agttgatcaa tttcctgatt
     3061 gctcaaagga tttggataga tacgttgaaa gaaagcaatc aactctttaa cttttaattt
     3121 ctgaacgatt acattttctt gagggaggta agccctagta tagtctaact tagaactcgt
     3181 cactggtaaa ccttgaatag atacttgacc gcttgtgacc agtttatctc caagcaaaca
     3241 atctaagagt gttgtctttc cggcaccatt tggaccaatt aaggcaacac attcaccatc
     3301 gttgatttca aaggagatgt tccgcaaaat ttccttgtct tttattttct tactcaattt
     3361 ctcaacttta accaccgtca tgagactctc ctttcaacca ctccattccc atagggaaaa
     3421 cgacgaaaat cataaatcca aactccaaag caccacgaat gaattggcaa agcaaggctt
     3481 gatcaaacca atcagtaaac atttccacta accataccaa gagtgacagg ccgataaaga
     3541 aatagatgat ccctctcttc attcttcaaa ctcctttttc acatctctga ctaatttcaa
     3601 accttctcta acaagccaag acatgattcc aaagccagca aagaccttcc aaggaaaatg
     3661 gtagaaatcc tcatccaatc ccgaaaacat gagataggtc ataactcctg ctactactaa
     3721 actcattgca ataatcattt tatttctcat ctcttcttcc cccatttcat actacaatta
     3781 tagtctttta aaatcagagg aggcagaagc ttctgtcact agaaaatatg acaaatgtca
     3841 taaaaaattc tgttcaaaac aagcaagata cattatacaa taaaacacaa tcagaaaaat
     3901 ctaaggcaac ttcctcaaaa gagatatcaa actcaattca caccataatg taaactaata
     3961 cttatttaaa atcaaaaaga gtagaaattt ttatcagaca aacacatata tagtgtattg
     4021 aatctataac agtaggcctt aaatactaaa atatttctat aaattaattt aactttcctg
     4081 atagagctgt tcatatctta tttcaattct ctatattata cgttgaacaa aacccttcta
     4141 ttgctttctt aaaaatttat aagagttata aaatctgtta aatttcaatg tgtataccta
     4201 aactacggta tttattgaaa agactggaga caaaaagtat acgctgccaa aatgaattac
     4261 tgaaaatcaa aaaagagaga accaaactga ttccctctta atgtatataa tatctagttt
     4321 taaaaataca cactcacata tctctgt
//
LOCUS       DAVBNO010000090         4335 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVBNO010000090 DAVBNO010000000
VERSION     DAVBNO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.090"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(1..648)
                     /locus_tag="VQ846_001986"
     CDS             complement(1..648)
                     /locus_tag="VQ846_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET3076506.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(757..1275)
                     /locus_tag="VQ846_001987"
     CDS             complement(757..1275)
                     /locus_tag="VQ846_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET3076507.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1502..2371)
                     /gene="rsmI"
                     /locus_tag="VQ846_001988"
     CDS             complement(1502..2371)
                     /gene="rsmI"
                     /locus_tag="VQ846_001988"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET3076508.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESSHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2374..2691)
                     /gene="yabA"
                     /locus_tag="VQ846_001989"
     CDS             complement(2374..2691)
                     /gene="yabA"
                     /locus_tag="VQ846_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET3076509.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2731..3621)
                     /locus_tag="VQ846_001990"
     CDS             complement(2731..3621)
                     /locus_tag="VQ846_001990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET3076510.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3618..4256)
                     /locus_tag="VQ846_001991"
     CDS             complement(3618..4256)
                     /locus_tag="VQ846_001991"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET3076511.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
BASE COUNT         1193 a          989 c          701 g         1452 t
ORIGIN      
        1 ctaggattct agttttgcag gaagtctaat gtaattctca agctgtgagt tcattatgat
       61 ttgtaaatcc gatatatctt cagcagctaa aaacacattt tgaaatgttc caagtccgtt
      121 ttcaccaaaa ctatattctc tcttactata cttactctta ttattctcta tatagttaga
      181 gtcaaggttc ttaacctctt gaagttcatt ttctttactt ctgaggttaa aatcttttac
      241 ttctgaagtt aagttttttg acttctgtaa gtcattttct tttacctgaa atatactcat
      301 aaagtcttta acataaatga tgttcggctt accaagtcca agccttactc tttcgatcag
      361 tcctattcct ttttttacat caagctcatc taatgttttt atggcagttg gctttgagat
      421 atttcttctt ttcataattt cttcgacagt aaaatagata aatactctgc cttccttgtc
      481 tatccagtta ttcttaaatg acattcctgt ctgtttaaga agtatggaat aaaggataat
      541 tgcttctgct gataagcccc gaaattcttc tctatcaact aatatctccg gtacttttaa
      601 gaagttaaat ctttccgctt ctctgttata aaaatattca aagtccatgc aagatcacct
      661 cccttcttaa aaatttgcaa agaaaaagac gatagttttt cttctatcgc ctttggtaac
      721 catttttatg aaatttttta aagttaaaaa tataatttat aatactatct tcttccattt
      781 tctgatttta gttctgaaag ttccaaaagg tgcaaccgta tttacatgaa taaacttata
      841 cacttcccaa gtcgctgttt tagtcgcttc atcagcccac tttctcatat gcggttcaaa
      901 taattcttcc tcactcaacg aatcaatcat tgcagagata gaattaatat tttcatttaa
      961 ttttgctttc aactcttgca gagataaatg agcgtaggta tctgtgaacc actgatataa
     1021 ttcaccaagt tgattccatt taaatttatc cgatggtgtt tttacttgaa gcccctttct
     1081 ttcatcttct tcccatttaa gaaccaagtt ggtccaacca acctgataag caaggttttc
     1141 tgctggagtt ctgtcaactt catcagctct cttatctttt aaattttctg gaatattatc
     1201 aaattctaaa atatatttct caaatgtttt ctctatctca gcttttagtt cttctttatt
     1261 ttcgtatgtt cgcaaaaaat caccttcttt ctgatttatc aatcctttga tttaaaatga
     1321 ccttattttt ctatttatac cttctaagaa cgctataact gctgctccaa ccatcggtat
     1381 tccgtatggg cttaataaaa aacttaatac caaagcttct ataccaagtg atacatcttt
     1441 atattaccat cactaattaa gttaaacaga aacagacaga attattacat tctgtctccc
     1501 tttattgttt ttcttcccaa tcgtggtagg cagcgtagag ttgactctta ttccactggt
     1561 aaatcttagc tacttcctta atagcttgat ttttcttcat gccttgctgg atacgggctt
     1621 ggatttctaa gaacaagtcc tcctcgtctt tttcctccac atcctgactg gcaccttcaa
     1681 caataagaag gcattcgccc ttgagtggcg tttcagcgat gctttccagc aattcagaaa
     1741 tggtacctct ttggtattct tcatagattt tagtcaattc cctgaccaag actaccgagc
     1801 ggtcaccgta gacttctagc atattttcca atgtatctgc cacacgatgg gacgattcat
     1861 aaaaaatctg agtttcagga tagtcttttt tcgagtcgaa aaattgcttt tgctgacctg
     1921 attttctcgg taaaaagccg taaaagatat gtggctgcgg cgctaaacca ctggcaatca
     1981 aggcagaaat tcctgcagag gcacctggaa cagtcacaac tgcaatttct tcctcaatag
     2041 ctgccttaac caaatcatga ccagggtctg aaatgctagg caaaccggca tcagagacct
     2101 gagcaatact ttgccctgct ttcaagaaac caatcaaatc aggaattttt tccttggcat
     2161 tgtgctcatg aaaactgatc tgcttggtgg aaatgtcaaa atgcttgagc aaaagacctg
     2221 tattgcgcgt atcctcagca gcaatccagt ccacttcttt caaggtctgg atagcgcgaa
     2281 aagtcatatc atctagattg ccaatcggcg ttgccactag atacagcttg ccatagggag
     2341 actgcccctt aaaacttttt tgaatttgca tgcctactcc ctgtataaca actcatcaca
     2401 gaacatacat tcttcgtcct gctctcgacg ttgtccataa aaatcattac atacgtgaaa
     2461 tccatcacgg taaatacgac ggacactttc gcgaacatgc ttggccttga cgggagcgtc
     2521 tgcttccacc tcacccaagc gttctcgcaa cttactattt tccaagcgaa gagctgtatt
     2581 ttcctctacc aggctcttga gatttttctt gatggcttcc acatcggcca aggttaccaa
     2641 taattgttgg gaaaaatcat ccagcgcgtc aaataattct tttttgtcca taaaacagct
     2701 ctttcctttc tttatcattc atttttgagt ttatatttct ttcaagacca gatattccat
     2761 ggcattttga aagctgacat tagcttgcca catttttcta gcttctagta aatcttgtag
     2821 aatcactctt actcttacct gcaagaggtc ctgcccacag agaacttcaa gaatccgtaa
     2881 aacctgatcc tgtttttcct tatcatctgc caagttggct aatttggcaa cctgtagata
     2941 actttctttt ttcttagcta ctaaccaagt cagcaggcgt tcactttcat cgaccaaggt
     3001 ccaaaaactt gcctgattag ccaacttttc tgcttcagct cgcgattgac taaacttagc
     3061 taaaagagtc gcttttttct taacaagtcc catttgttct aagagtaaga taagtttttc
     3121 ttcttgcttt ttaaagtgga agatctgggt ccgacttcgg attgtcggta acatcttttc
     3181 ctcatcgcta gtcaagaaga aaatataaac ttcactctgg ggttcttcga tgaccttgag
     3241 cagagaattg gctgcgttgg gatgcatttt atccgcttgc tcgatgataa agacctgttg
     3301 ctggctttca atccctgctt gagaaaactg tcccaccaat tccctaatgc gttctgtctt
     3361 gatgacctga ttgactggct taatcaaggt gacatcggga aattctccct gttcaatcag
     3421 cttgcaactt cggcatttct cacatggtaa gacgccaact ttatccgtac aaaagaggct
     3481 cttagctaaa aattgcgcta tttccaagct tccaaagaaa cctgaaaaga gataggcgtg
     3541 attgagctgg tcttgttcta agatacggac aaaacggtca aactgagctg gttgccaagc
     3601 ctttagttga tcttgtttca tttggccaag cccattccgt caaacaagac agccttggta
     3661 gtttccacaa cttgctccaa agggagacta gcatcaatct tgacaatgcg atttccctct
     3721 ttatccagaa gagaaaggta gccttgacga acttttttat gcaagtccaa cccttccaaa
     3781 tccaaacgat taacctcgcg gtcactatta gcagcaatac gagccagccc ttcttccacc
     3841 tcgatgtcaa aatagagtgt caaatcgggt ttgaggccat ctgtcgcaaa ctgattgagc
     3901 cagtcaatgg cttcaatatc taagccacga ccaaatccct gataggcaac agaactatcg
     3961 ataaaacgat ccatgatgac caacttgcca gcttcaaggg ctggaagaac tttttccacc
     4021 aaatgctgtc tgcgactggc aatatagaga agtagctctg ttttagcatc catctgagta
     4081 tgacttggat ccaaaatcac ttcccgaatc ttctccccaa tcaagactcc gccaggttca
     4141 cgggtcgtca gcacctttac tcctttttcc tctaaaattg gtagcagagc ctctaaaaca
     4201 ctggtcttgc ctgctccctc tggtccctca agagagacta aaaatccttt tgacatgtct
     4261 aactcatttc ttttttacta cttctattct atcaaaaaaa taggggtttg tgacaatctt
     4321 tttgtcttct cattt
//
LOCUS       DAVBNO010000091         4287 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVBNO010000091 DAVBNO010000000
VERSION     DAVBNO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.091"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            1..142
                     /locus_tag="VQ846_001992"
                     /pseudo
     CDS             1..142
                     /locus_tag="VQ846_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(135..308)
                     /locus_tag="VQ846_001993"
     CDS             complement(135..308)
                     /locus_tag="VQ846_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET3076512.1"
                     /translation="MNIKKRVLSAGLTFASVLLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYTDNIVN"
     gene            complement(467..2074)
                     /locus_tag="VQ846_001994"
     CDS             complement(467..2074)
                     /locus_tag="VQ846_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET3076513.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2227..3711
                     /locus_tag="VQ846_001995"
     CDS             2227..3711
                     /locus_tag="VQ846_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET3076514.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIITGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3806..>4287
                     /locus_tag="VQ846_001996"
     CDS             3806..>4287
                     /locus_tag="VQ846_001996"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET3076515.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1240 a          980 c          816 g         1251 t
ORIGIN      
        1 cttttacctc ttcctcccta ctcacctgag tacaatccta ttgagaaaac atgggctcat
       61 atcaaaaagc acctcataaa aggtattacc aagttgcaat accttttatg aggctctttt
      121 gtcttgttct tgtttcaatt gactatatta tcagtgtaat agtctaatag atagttaaat
      181 gtagttggat ttccactaaa ggttgatgag taagtttttg tatctgaacc tgattggccg
      241 caagcagcta aaagcaaaac agatgcaaaa gtcaggcctg cactaaggac acgcttcttt
      301 atgttcatct tctttctcct taatagtggg aatttgtaaa gttaattgaa tttcgagaat
      361 gaaagtttat aaaccttggt tataaaaaac aaaggatttc tgctttttat acagtcctcc
      421 cttgttttta tatgatttca attttaaatt tttctgcaaa aaatatttat agtaattcca
      481 cacagaaagc atcccatgga actaagattt gtttttcaaa gacttcttga gctagggtgt
      541 tttcaatcaa gacagatttg acttttcctt ctactgtcaa gtcttgctct tcattggaca
      601 agttagccac aactaggaag cgacggtcgc catccttacg tatataagca aagaccttat
      661 cagccgtatc aagcaattca aagtcagctc gaactagcca gctattctcc ttgcgaattt
      721 ggaccagttt ctgataggta tagaaaatag aatctggatt tgccagcgct tcttggacat
      781 tgatcatctc gtaatttgga ttaaccgcca accaaggttg acctgttgag aaaccagcgt
      841 ttttgctctc gtcccattgc ataggggtac gggcattgtc acgtccaata acacggatac
      901 tgtccatgat ttcttcaatc ggaacacctt tttcaagagc ctcacgcgca tagttgagag
      961 attcaatatc ttctacttga tccagcgttt caaagggata gttggtcatg ccaatttcct
     1021 caccttggta gatataagga gttcctctca tgagatgaag caagatggca aaggctttgg
     1081 cagatttttc gcggtattct tggtcatttc cccagattga gacaatacga gggaggtcat
     1141 ggttgttcca gaagagggaa ttccagccgt cctcaactcc taactctgtc tgccatttgt
     1201 tgaagatttc ttttaactta gcgatattca gctctttttg atagtgccat ttaggctgac
     1261 cttcctgata ctgaagaccg atatgttcaa actggaagac catagacaat tcttgcccct
     1321 ttggatcaga gtagagcttg gcaatctctg gcgttgctcc ccaagtctcc cctactgtca
     1381 agagatcctt atctccaaag gtcgcctgat tcatttcctt gagataggga tggagcatag
     1441 gaccattatt gactaccttc tcgtcaggaa ttttgccaat catgtcaata acatccatac
     1501 ggaaaccacc aataccctta tcaatccaga agttcatcat ctcataaatt ttctggcgaa
     1561 gtttttcatt ttcccagttg agatccggct gtttcttgct gaaaaagtgg agatagtatt
     1621 gacctgactt ttcatcgtat tcccaagcag acccactaaa gatagaatct aggtcattgg
     1681 gttcatcgcg ccagatatag tagtctcgct cagggctatt aggattttca caggcctcta
     1741 caaaccaggc atgctcatcc gaggtatgat tgaccaccaa gtccatgatg atacggatat
     1801 cacgcttctt agcttctgca atcagttgat ccatatcctc catggttcca aaaatcgttg
     1861 caatcgcttg ataatcagca atatcgtagc cattatcatc catagggctg tcataaacgg
     1921 gagaaagcca aattgctgtg attcctagct tggctagata gtccaactta ctggtaattc
     1981 ctggcaaatc gccaattcca tctccattac tatccataaa actctttgga tagacttgat
     2041 agactacggc attgtgccac catttttctt gcatcttctt acctcattat tttcataatc
     2101 tatagtctat gatagcactt tttaaaattt tgtgcaagcg tttgcttaat ctttttaatt
     2161 tcttttttag ctccgtagtt tcctaacttc caatttttta ttataataga gtccagaggt
     2221 aaaaaaatga aaaaacaagc ttttagttct gaacaatatt tgaatttaca acgcgaccat
     2281 attttggagc gcattaacca atttgacggc aagctctact tagagtttgg cggtaaaatg
     2341 ttagaagatt tccacgctgc tcgtgtcctt cctggttatg aacctgacaa caaaatcaag
     2401 ctcttgcaag aattgaaaga gcaggttgag gttgtgattg ccattaatgc tagcaacatt
     2461 gaacattcca aagcacgtgg cgacttaggc atttcttatg accaagaagt tcttcgtttg
     2521 attgacaaat tcaatgaatt agggattttt gttggttccg ttgtcattac acaatacgct
     2581 ggccaacccg ctgcagatgc cttccgcaat caacttgaga aaaacggaat tgattcttat
     2641 cttcattatc caatcaaagg atatccgacg gatatggatc acatcatttc cccagaaggc
     2701 atgggcaaaa acgactacat caaaaccagt cgcaacttga ttgtcgtaac cgctcctgga
     2761 cccggttctg gaaaattggc aacgtgtatg tccaatatgt accacgacca aatcaatggc
     2821 atcaagtctg gctacgctaa atttgaaacc ttccctgttt ggaatcttcc ccttcatcac
     2881 ccagttaact tggcttatga agctgccaca gctgaccttg atgatgtcaa catgattgac
     2941 cccttccatc ttcaaaccta tggagaaacc actgtcaact acaaccgtga tatcgaaatc
     3001 ttcccagtgc tcaagcgcat gttggaacgt attctcggaa aatcaccata cgcttcaccg
     3061 acagatatgg gtgtcaacat ggttggtttc gctattacag atgacgaggc tgctgtcgaa
     3121 gcttctaaac aagaaatcat ccgccgttac tatcaaacag ttcttgactt caaagctgaa
     3181 aaagttggcg aagctgccgt caagaaaatt gagttactca tgaacgacct cggtatcaca
     3241 cctgcagacc gtaaggttgc tgtcgttgcg cgccaaaaag cagaagaaac tggtggacca
     3301 gccctagcct ttgaattgcc aaatggggaa atcatcactg gtaagaactc agaactcttt
     3361 ggccctacag ccgctgcctt gatcaatgcc atcaaaaaat cagctgacat cgctaaagaa
     3421 gtaaaactaa tcgagcctga agttgttaag ccaatccaag gtcttaaaat cgatcatctc
     3481 ggtagccgca atccacgcct tcattcaaat gaaatcctga ttgcacttgc tatcacagct
     3541 acagaaaatc ctgatgctgc ccgcgctatg gaagaactcg gcaacctcaa aggaagcgaa
     3601 gcccactcaa ccatcatctt gactgatgaa gacaagaatg tccttcgtaa actgggtatc
     3661 aacgtaacct ttgacccata ctaccaatac gaccgcttat atcgtaagta aagatttcaa
     3721 cctctccagc ctcggagagg ttttctctct gtctcaggag caacctttat gatataatga
     3781 gagaagaaaa ctgaaaggat ttaccatgtc aaaagaagtt attgtcgaaa gttttgaact
     3841 tgaccacacc attgtcaaag caccctatgt tcgcttgatt ggggaagaaa caggaccaaa
     3901 aggagacatc atctccaatt atgatattcg cttggtgcaa ccaaacgaag actctatccc
     3961 tactgccggc cttcacacta tcgagcacct cttagccaaa ctcatccgta cccgcattga
     4021 cggcatgatt gactgttcac catttggttg ccgcacaggc ttccacatga ttatgtgggg
     4081 acgtcacacc agtgctaaaa tcgcagctgt tatcaaggat tcgctcaagg aaatcgctga
     4141 aactactact tgggaagatg tcccagggac aaccatcgaa tcttgcggaa actacaagga
     4201 ccacagcctc ttctctgcta aagaatgggc aaaactcatc ttggaacaag ggatttcaga
     4261 tgatgccttt gaacgtcatg tgattta
//
LOCUS       DAVBNO010000092         4193 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVBNO010000092 DAVBNO010000000
VERSION     DAVBNO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.092"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            720..1712
                     /locus_tag="VQ846_001997"
     CDS             720..1712
                     /locus_tag="VQ846_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET3076516.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQNNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINKEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1714..2532
                     /locus_tag="VQ846_001998"
     CDS             1714..2532
                     /locus_tag="VQ846_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET3076517.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2534..3319
                     /locus_tag="VQ846_001999"
     CDS             2534..3319
                     /locus_tag="VQ846_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET3076518.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3358..3636
                     /locus_tag="VQ846_002000"
     CDS             3358..3636
                     /locus_tag="VQ846_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET3076519.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTGFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3621..3818
                     /locus_tag="VQ846_002001"
     CDS             3621..3818
                     /locus_tag="VQ846_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3076520.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1171 a          717 c          904 g         1401 t
ORIGIN      
        1 gctatatatt atggtatcga atcttcatca gagtgataaa attaatcaat taatatctga
       61 ttacaaacag aatatgaaag ctttttatat cactattgaa aaatttatac gagatgatga
      121 aagccttaag tgttatttta taaaggttat ttcaagtcgt tccaaggtaa caagtctaga
      181 tcagattgaa gctgataaaa cgatacaaag aaaatattca agtgagctaa aaaaatttat
      241 tggattttat aatgagatta tttgtgagga aaatagtttc ctacatgtac gaaagaggtg
      301 gtcgagttgg tttaggtagt cggtgcgtga gttgataatt ctcagggtat ggacttcttt
      361 ttcatgaatg aggtaaaaga gcaggtattg tttagagaca atcattctga gcatattttc
      421 tggatagagg gagtatccga ttttatgatc aaagttaata ccgccctctg gtgagaagat
      481 gagtaggttg gtaatttaaa ctattaaaca gaatttttga ttaaaagtat tattttatga
      541 gagaaatcct aattttacaa tccataggca aacgcttgca tttcgttttt tattggacta
      601 taataggttg gtataaagcc ttttgtagta ataaaatgta gaaggtgtag aaagtaagga
      661 tttagaatat ttgtagttaa aaacacaatg ttgctattcc ttacgatagg gagatagata
      721 tggcaatgat agaagtggaa catcttcaga aaaattttgt gaagactgtt aaggaaccgg
      781 gcttgaaggg ggctttgcgc tcctttattc atcctgaaaa gcagaccttt gaagcggtca
      841 aggatttgac ctttgaggtt ccaaaagggc agattttagg atttatcggg gcaaatggtg
      901 ctgggaagtc gacaaccatt aaaatgctga caggaatttt gaaaccaaca tctggttttt
      961 gtcggattaa cggcaagatt ccccagaaca atcggcaaga ttatgtcaaa gatattggcg
     1021 tagtctttgg acaacgcacc cagctatggt gggatttggc tctgcaagag acctacactg
     1081 tcttaaaaga gatttatgat gtgccaaact cgctctttca taagcgcatg gactttttga
     1141 atgaagtctt ggatttgaag gactttatca aggatcccgt gcggactctt tcactgggac
     1201 aacggatgcg ggcggatatt gcggcctcct tgctccacaa tcccaaggtt ctttttttag
     1261 atgagccgac cattggtttg gacgtttcgg ttaaggataa tattcgtcgg gcaattactc
     1321 agatcaataa agaggaagaa actaccattc ttttgaccac tcacgatttg agtgatattg
     1381 agcaactttg tgatcggatt ttcatgattg acaaggggca agagattttt gatggaacgg
     1441 tgagccaact caaggagacc tttggtaaga tgaagactct ctcttttgaa ctgctaccag
     1501 gtcaaagtca tctcgtctct cactatgaag gtctgtctga tatgaccatt gatagacaag
     1561 gaaacagcct caacattgaa tttgatagtt ctcgctacca gtcagctgac attatcaagc
     1621 aaaccctatc tgattttgaa atccgcgatt tgaagatggt ggatacggat attgaggata
     1681 ttatccgtcg cttctaccga aaggagctct aggatgatca aattgtggag acgttataaa
     1741 ccctttatca atgcaggggt tcaggagttg attacttacc gagtcaactt tattctctat
     1801 cggattggcg atgtcatggg ggcttttgtg gccttttatc tctggaaggc tgtctttgat
     1861 tcttcgcaag agtctttgat tcagggcttc agtatggcgg atatcaccct ctacatcatc
     1921 atgagttttg tgaccaatct tctgactaga tccgattcgt cctttatgat tggggaggag
     1981 gtcaaggatg gctccattat catgcgtttg ttgcgaccag tgcattttgc ggcctcctat
     2041 cttttcaccg agcttggttc caagtggttg atttttatca gcgttggcct tccattttta
     2101 agtgtcattg tcttgatgaa aatcatatcg ggtcaaggta ttgtagaggt gctaggatta
     2161 actgtccttt atctttttag cttaacgctc gcctatctga ttaacttttt ctttaatatt
     2221 tgctttggat tttcagcctt tgtgtttaaa aatctttggg gttccaacct acttaagact
     2281 tccatagtgg cttttatgtc ggggagtttg attcccttgg cattctttcc aaaggttgtt
     2341 tcagatattc tctccttttt gcctttttca tccttgattt atactccagt tatgatcatt
     2401 gttggaaaat acgatgccag tcagattctt caggcactcc ttttgcagtt cttctggctc
     2461 ttagtgatgg tgggattgtc tcagttgatt tggaaacggg tccagtcctt tatcaccatt
     2521 caaggaggtt agtatgaaaa aatatcaacg aatgcatctg atttttatca gacaatacat
     2581 caaacaaatc atggaatata aggtggattt tgtggttggt gtcttgggag tctttctggc
     2641 tcaaggcttg aatctcttgt ttctcaatgt catctttcaa catattccat tcctagaagg
     2701 ctggaccttt caagagatag ctttcattta tggattttcc ttgattccca agggaatgga
     2761 ccatctcttt tttgacaatc tctgggcact agggcaacgc ctagtccgaa aaggagagtt
     2821 tgacaagtat ctgactcgtc ccatcaatcc tctctttcac atcctagttg aaacctttca
     2881 gattgatgcc ttgggtgaac tcttagtcgg tggtatttta ttgggaacaa cagtgaccag
     2941 cattgtttgg actcttccaa aattcctgct tttcctagtt tgtattcctt ttgcgacctt
     3001 gatttatact tctcttaaaa tcgcaacagc cagtatcgcc ttttggacta agcagtcagg
     3061 cgccatgatt tacatcttct atatgttcaa tgactttgct aagtatccga tttctattta
     3121 caattctctt cttcgttggt tgattagctt tatcgtgcct ttcgccttta cagcctacta
     3181 tccagctagc tatttcttac aggaaaagga tgtgttcttt aacgtaggag gtttgatgtt
     3241 gatttctctg gttttctttg ttatttccct taaactttgg gataagggct tagattccta
     3301 cgaaagtgcg ggttcgtaaa agctaaagta agactaaaat caagaaagaa acttatgatg
     3361 tttgtaattg aagaagtcaa ggatgaaaat caaaaaaagg cagttgtagc tgaggttttg
     3421 aaggatttgc cagaatggtt tggaatccca gaaagcacac aagcctatat agaaggaacc
     3481 acgacactgc aagtttggac cgcctatcag gagagtgatt tgactggatt tgtaagctta
     3541 tcctattcga gtgaagattg tgcagagatt gattgtctcg gcgtaaaaaa gcttatcaag
     3601 gtagaaaaat tgggagccaa ttgcttgcta ctttagagag tgaagctcgt aaaaaagttg
     3661 gttatctgca ggtcaaaaca gtagcagaag gttctaataa agattatgat cgaacaaatg
     3721 acttttatcg aggtcttggc tttaaaaagt tagagatttt tcctcaacta tggaatccgc
     3781 aaaatccttg tcagattttg attaaaaagc ttgaataata ttatttgaca tctattctca
     3841 gagtgctata ctgtaagtgt aatcgccgat ttagctcagt tggtagagca aggcactcgt
     3901 aaagcctagg ttataggtag ataaacgact gaggatttga aaaaatagat aggtagaaga
     3961 taaccgttaa gccttattct tagcggttat ttatattgtt taatagcgct gatatttcat
     4021 caattatgcc tgttttcgtg tttctggtag ttgttcaagt ttattgctac tatttttgat
     4081 ggtatgaatg tgcttataat gtatcccggt taacgaaagt tttggactta tactcttaga
     4141 aaatctcttc aaaccacgtc aacgtcgcct tgccgtgcgt atggttactg act
//
LOCUS       DAVBNO010000093         3984 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVBNO010000093 DAVBNO010000000
VERSION     DAVBNO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.093"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(382..915)
                     /locus_tag="VQ846_002002"
     CDS             complement(382..915)
                     /locus_tag="VQ846_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET3076521.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1004..1780)
                     /gene="recX"
                     /locus_tag="VQ846_002003"
     CDS             complement(1004..1780)
                     /gene="recX"
                     /locus_tag="VQ846_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET3076522.1"
                     /translation="MKITKLEKKKRLYLMELDGQQTSYITEDTIVRFMLSRDKVISKE
                     ELTEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPSRALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYSKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            complement(1790..2566)
                     /locus_tag="VQ846_002004"
     CDS             complement(1790..2566)
                     /locus_tag="VQ846_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HET3076523.1"
                     /translation="MNSKTPRFSILDFPEQLRSYIKGANLSDSSSHSSATVLYIDSGY
                     YLKIDQKGRLAQETKIASWFETEGLGTPVITYLSANKDFLLTKEAIGSNALAFLNQPE
                     RLCQTLAQALNKLHSLKPQSFPSENHLKRYKEKALKNYQKGTFYNKTLLPQFHIHSRE
                     EAYQLIQEKGYILKADAFIHGDACLPNFILKDASHFSCFIDLGLAGFSDRHIDLFWAI
                     WSLHYNLQDATYAELFLDYYGREYVNMDKLRLVAAFEAFR"
     gene            2623..3978
                     /gene="rlmD"
                     /locus_tag="VQ846_002005"
     CDS             2623..3978
                     /gene="rlmD"
                     /locus_tag="VQ846_002005"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET3076524.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKENHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
BASE COUNT         1266 a          698 c          745 g         1275 t
ORIGIN      
        1 atttcatttt actatactat tacgaaatta gatatataaa tgaaaacttg cctcaaggta
       61 ggtttttttg ctagtagaaa agcgatagac agaaattgtt ggactcttaa aaaaatgaag
      121 ttttgctcaa aagagcttct taaattaaaa cgagcaatta tcagttcaaa tctgtttcaa
      181 aagaagaacg gataaattaa tcgatgcttt gtttttgagt taggaagtta agcatgccaa
      241 actgagtcgt aggaataaat ctctttctag taatgtgttg taactctgct ataatggatt
      301 tattcctttt tgtgtttaca caatttattt tatactacca aaaaaacaag gtcaggattt
      361 tgttcctgac ctttgacaac tttaccgatt ctttagttct acatagcgct tgtaccaaat
      421 gtttacatag gcttctgaga aaggaccacg tccattgtta atccaatcaa caagaatttt
      481 gacatgttct tttaaaatat agtccaagtc atcagaataa ttcattttgc gtttgtgacg
      541 ctcgtactct tcaacgtcca agagacgttt ttccccatct gtaaaaattt taacatccaa
      601 atcgtaatca atatacttca gtgcttcttc atccagatag taggggctag ccatattgca
      661 atagtaagaa gttccattat cacgaatcat ggcaatgata ttaaaccaat atttcttgtg
      721 aaagtaaaca atagccggtt ctcgagtgac ccaacgacga ccatcacttt cggtaacaag
      781 tgtatgatcg ttgacaccaa taatggcgtt ttctgttgtt tttagtacca tggtgtcccg
      841 ccaagttcgg tggagactcc catcatgctt ataactttga attgtaataa agtcgccttc
      901 ttttggaagc ttcataacta accaactttc tacaatttat aagtttatca tttactattg
      961 taccataaaa ttacctaaaa tctgtgaatt tcacttggaa atattaaaga tattctctaa
     1021 gagcgcttgc tatatccgaa aaatcgtagc cctttcgtgc taaaacttga gttaaacgct
     1081 gcttcagttc gtatccttca tactttcgag catacttact atattgttta tcaagttcct
     1141 tgaagatgag ttcttgagtc gtttcttggt caacttggct atctaagtcg tcaaaggcaa
     1201 ttttagcatc agaataagag aagcccttgt tagtcaagtt ctggataatc ttatcttgca
     1261 gggcacgaga tggaagtttt ccctcatatt ttttcaatag tttattagct acacgttgag
     1321 caacttcaga aaaatcaaat tctttcaagt tctcttctat agtagatttt gaaatccctt
     1381 tttgagctag tttctgagtc agcacataag gacccttgtc tcctgaaagt tgattggtat
     1441 tgatgatagc ataagcgtac tgaccatcat taatccactt atcttcttta agattagcaa
     1501 taacttgaga aacgatgttt ttatcaatat cgtatttttt cagatattct ctgacttctt
     1561 tttcagtgcg tgctttaaag gataagtggt agagagcgag attcttacca taagaaaact
     1621 gggcaaaatc ctgaatctcg gtcagttcct ctttactaat gaccttatct cgagacagca
     1681 taaaacggac aatggtatct tccgtgatat aagaggtttg ctgtccatct agttccatca
     1741 agtagagacg ttttttcttt tcaagttttg tgattttcat aatttccttt catctaaatg
     1801 cttcgaaagc tgcaacaagt cgtagtttat ccatatttac atattctcta ccgtaatagt
     1861 cgagaaataa ttcagcatac gtagcatctt gtaggttgta atgaagggac caaattgccc
     1921 aaaagaggtc gatatgtcgg tcactaaaac cagccaatcc caagtcaata aagcaagaaa
     1981 aatgagaggc atccttcaag atgaaatttg gcaggcaggc atctccatga ataaaagcat
     2041 cagctttgag aatgtaacct ttctcttgta tcagttggta agcttcttca cgactgtgaa
     2101 tgtgaaattg aggcagaaga gtcttgttat aaaaagtccc cttctgatag ttttttaagg
     2161 ctttttcttt ataacgcttc agatgatttt ccgacggaaa agattgtggt ttaagactgt
     2221 gaagtttgtt taatgcttga gccagggttt gacagagtct ttcgggttga tttaaaaaag
     2281 cgagagcgtt gctgccaatg gcttctttag tcagaaggaa gtccttattt gctgataaat
     2341 aggtaatcac tggagttccc aatccttcag tttcgaacca acttgcaatc ttagtttctt
     2401 gagctaatct gcctttttga tctattttta aatagtaacc tgaatctata tataggacag
     2461 ttgcacttga atgagaagaa ctatcagaga gattagctcc cttgatatag cttcgtaatt
     2521 gttcagggaa atctagtata ctaaagcgag gtgtttttga attcatgctt ttattgtaac
     2581 atattcttaa atatcttaga aactccatgg tataatagac atatgaatct gaaagtgaaa
     2641 caaaaaatac cattaaaaat caagcgcatg ggaattaacg gtgagggaat cggcttttac
     2701 caaaaaacat tagtctttgt accaggagct ctcaaaggcg aagatatcta ttgtcagatt
     2761 acttctatta gacgcaactt tgttgaagca aaattactga aggtcaacaa gaagtctaaa
     2821 tttcgaattg tgccatcttg tactatttat aatgaatgcg gaggctgcca aatcatgcac
     2881 ctgcattatg ataagcagct ggagttcaag acggacttac ttcatcaagc gctgaaaaaa
     2941 tttgctcctg caggatatga aaattatgaa attcgtccaa ctattggaat gcaggaacca
     3001 aaatattaca gagctaagtt acaatttcag actcgaaaat ttaaaaatca ggtcaaggcg
     3061 ggcttatatg cacaaaactc tcactattta gtagagttga aagactgcct ggtacaagat
     3121 aaggaaaccc aagtgattgc taatcgctta gcagaattac ttacttatca ccagattcca
     3181 atcacggatg agagaaaagt tctaggtgtc cgtactatta tggtccgacg cgcgagaaag
     3241 accggacagg ttcagattat tattgttaca aaccgccagc ttaatttaac tcaattggta
     3301 aaagagttgg ttaaagattt cccagaagtt gtgacagtag ctgttaatac aaatacagct
     3361 aaaaccagtg agatatatgg tgaaaagaca gagattatct gggggcaaga gagtattcaa
     3421 gaaggtgtac tcaattatga attttcacta tcccctcgag ctttttatca actaaatcct
     3481 gagcaaacag aagtcctcta tagcgaagca gtaaaagcgc tggatgttga taaagaaaac
     3541 catttgattg acgcttattg tggagttgga acgattggat ttgcctttgc aaagaaagta
     3601 aaaacactca gaggtatgga tattattcca gaagctattg aagatgccaa gcgaaatgct
     3661 aaaagaatgg gatttgacaa tactcattat gaagctggaa cggcagaaga gattattcct
     3721 cgttggtaca aggaaggcta ccgagcagat gctttgattg ttgacccacc acgtacaggt
     3781 ctggatgata agttattaga tactattctt acttatgtac cagaaaaaat ggtttatatt
     3841 tcttgtaatg tttcgacctt ggctcgtgat ttggtacgct tagtagaagt ctatgatctt
     3901 cattatatcc agtcggtcga tatgttccca catacagctc gaactgaagc tgttgtaaaa
     3961 ttaataaaaa aagtttaaaa aagg
//
LOCUS       DAVBNO010000094         3893 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVBNO010000094 DAVBNO010000000
VERSION     DAVBNO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.094"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(112..1782)
                     /locus_tag="VQ846_002006"
     CDS             complement(112..1782)
                     /locus_tag="VQ846_002006"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET3076525.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2012..2701
                     /gene="coaB"
                     /locus_tag="VQ846_002007"
     CDS             2012..2701
                     /gene="coaB"
                     /locus_tag="VQ846_002007"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET3076526.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2713..3264
                     /gene="coaC"
                     /locus_tag="VQ846_002008"
     CDS             2713..3264
                     /gene="coaC"
                     /locus_tag="VQ846_002008"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET3076527.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETLDEKTL"
     gene            3248..3811
                     /locus_tag="VQ846_002009"
     CDS             3248..3811
                     /locus_tag="VQ846_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET3076528.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
BASE COUNT         1171 a          874 c          693 g         1155 t
ORIGIN      
        1 gtttgaaaag attttcgaag agtataaatc ttattgatga actgcttgca gtctgagaaa
       61 aaatgagctt ggatattatt tccaaactca cttaaagtca atttcaatcc actagaacaa
      121 gcctagcaca gttccatcgc tttcaacatc catgttgaga gctgctggac gttttggaag
      181 acctggcatg gtcataacat caccagttaa ggcaacgatg aagcctgcac ctaattttgg
      241 taccaattca cgaatggtaa tttcaaagtt ttctggtgct ccaagcgcat ttggattgtc
      301 tgagaaactg tattgagttt tagccataca aattggcaat ttgtcccaac cgttttgaac
      361 gatttgagca atttgtgttt gagctttctt ctcaaagttc actttgctac cacgatagat
      421 ttcagtgaca attttttcaa tcttttcttg gacagaaaga tcattatcgt acaaacgttt
      481 atagttagct ggattttcag caattgtctt aacaactgtt tcggcaagtg ctactccacc
      541 ttctgctcca tcagcccaga cactagccaa ttcaactggt acatcgattg aggcacagag
      601 ttcttttaag actgcaattt cagcttctgt atcagataca aattcgttaa tagctacaac
      661 tgctggaata ccgaacttac ggatattttc aacgtggcgt ttcaagttag caaaacctgc
      721 acgaactgcc tctacatttt cttcagtcag agcgtcttta gccacaccac cattcatctt
      781 aagggcacga agggttgcga caataacaac tgcatctgga gatgttggca agtttggtgt
      841 cttgatatca aggaatttct cagcaccaag atccgcacca aaaccagctt cagtaacagt
      901 gtaatcagcc aagtgaaggg ctgttgtcgt cgccaaaaca gagttacagc catgagcgat
      961 attggcaaat ggaccaccgt gtacaaaggc aggtgtaccg taaattgtct gaaccaagtt
     1021 tggcttaata gcatccttca aaatcaaagc caaggcaccc tcaacctgca aatcacctac
     1081 agaaacaggc gtacggtcat agcgataacc aataacgata ttcgccaaac gacgtttcaa
     1141 gtcctcgatg tccgttgcca agcaaagaat tgccatgatt tctgaagcaa ctgtaatatc
     1201 aaaaccgtcc tcacgtggaa taccgtttag aggaccacca agaccaacag tcacatggcg
     1261 gagcgcacgg tcgttcaagt ccacaacgcg tttccagagg atacgacgtt gatcaattcc
     1321 cagctcattc ccttggtgca agtggttgtc aatcaaggca gaaagggcat tgttggcagt
     1381 tgtaatagca tgcatatctc cagtaaagtg gaggttgatg tcttccattg gcagaacttg
     1441 tgcataccca ccaccagcag caccaccctt gatccccatg actggaccaa gagacggttc
     1501 gcggatagca atcatggttt tcttgccaat cttgttcaag gcatccgcaa gaccaatggt
     1561 aagcgtcgac tttccttcac ctgcaggtgt tgggttgatg gcagtaacca agatcaattt
     1621 accgactgga ttgctctcaa ctgcacgaat tttatcaaag ctgagtttag ccttgtactt
     1681 tccgtacaac tccaaatcgt cataagaaat accaagtttc tctacaacat caacaattgg
     1741 cttcaactca atactctgtg cgatttcaat atctgttttc attcaaaatt cctctaacct
     1801 cttatatgat aattcattat atcacaaaac aagattttta acatcctaaa actctctaaa
     1861 cgttcgtaaa tatctctgtt tttaagactt ttagagtcct ttcttaaatt ttatatggct
     1921 ttatagtttg aaactataat aaatcttcgt ttttaccaaa aatttatcac tttcatttta
     1981 cttaccgctt atttttgtgt acaatagtgc tatgaaaatt ttagttacat cgggcggtac
     2041 cagtgaagct atcgatagcg tccgctctat cactaaccat tctacaggtc acttggggaa
     2101 aattatcaca gagactttgc tttctgcagg gcatgaagtt tgtttaatta cgacaaaacg
     2161 agctctgaag ccagagcctc atcctaacct aagtattcga gaaattacca ataccaagga
     2221 ccttctaata gaaatgcaag aacgtgttca ggattatcag gtcttgatcc actcaatggc
     2281 tgtttctgac tacactcctg tttatatgac agggcttgag gaagttcagg ctagctccaa
     2341 tctaaaagaa tttttaagca agcagaatca tcaggccaag atttcttcaa ctgatgaggt
     2401 tcaggttttg ttccttaaaa agacacccaa aatcatatcc ctagtcaagg aatggaatcc
     2461 tactattcat ctgattggtt tcaaactgct ggttgatgtt accgaagatc atctggttga
     2521 cattgcacga aaaagtctta tcaagaatca agcagattta atcatcgcga atgacctgac
     2581 tcaaatttca gcagatcagc accgagctat atttgttgag aaaaatcagc ttcaaacagt
     2641 ccagactaaa gaagaaattg cagaactcct ccttgaaaaa attcaagcct atcattctta
     2701 gaaaggaaaa ctatggcaaa cattctcttg gctgtaacgg gttcaatcgc ctcttataag
     2761 tcggcagatt tagtcagttc tctaaaaaaa caaggccatc aagtcactgt cttaatgact
     2821 caggctgcta cagagtttat ccaacctttg acactacagg tactctcaca gaatcctgtc
     2881 cacttggatg tcatgaagga accctatcct gatcaggtca atcatatcga acttggaaaa
     2941 aaagcagatt tatttatcgt ggtacctgca actgctaaca ctattgcaaa actagctcac
     3001 ggatttgcgg acaacatggt aaccagtaca gctctagccc taccaagtca tattcccaaa
     3061 ctaatagctc ctgctatgaa tacaaaaatg tatgaccatc cagtaactca gaataatctg
     3121 aaaacattag aaacctacgg ctatcagctg attgctccta aggaatccct actagcttgt
     3181 ggagaccacg gacgaggagc tttagctgac ctcacaatta ttttagaaag aataaaggaa
     3241 actctcgatg aaaaaacgct ctaatattgc acccattgct atcttttttg ctaccatgct
     3301 cgtgatacac tttctgagct cacttatctt taaccttttt ccatttccaa tcaaaccgac
     3361 cattgttcat attcctgtca ttattgccag cattatttat ggtccacgag ttggggttac
     3421 acttggattt ttgatgggat tacttagctt gacggttaac acgattacga ttctaccgac
     3481 aagctacctc ttctctccct tcgtaccaaa cggaaacatc tactcagcta tcattgccat
     3541 tgtcccacgt attttgattg gtttaactcc ttacttagtc tataaactga tgaaaaacaa
     3601 gactggtctg attttagctg gggctcttgg ttccttgaca aatactatct ttgtccttgg
     3661 aggaatcttc ttcctatttg gaaatgttta taatggaaat atccaacttc ttctggcaac
     3721 cgttatctca acaaattcaa ttgctgaatt ggtcatttct gcaattctaa ccctagccat
     3781 tgttccacga ctacaaacct tgaaaaaata aaaacaatct ccgctttcaa acctgaaggt
     3841 ggagattttg ctctatacag tttcttttta atgaaatctt tactatagta aaa
//
LOCUS       DAVBNO010000095         3774 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVBNO010000095 DAVBNO010000000
VERSION     DAVBNO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.095"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            130..1675
                     /locus_tag="VQ846_002010"
     rRNA            130..1675
                     /locus_tag="VQ846_002010"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1727..1799
                     /locus_tag="VQ846_002011"
     tRNA            1727..1799
                     /locus_tag="VQ846_002011"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1760..1762,aa:Ala,seq:tgc)
     gene            1924..>3774
                     /locus_tag="VQ846_002012"
     rRNA            1924..>3774
                     /locus_tag="VQ846_002012"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1079 a          763 c         1109 g          823 t
ORIGIN      
        1 agtgacaaag acctttgaaa actgaacaag acgaaccaat gtgcagggcg ctacaacata
       61 agttgtagta ctgaacaatg aaaaaaacaa taaatctgtc agtgacagaa atgagtgaga
      121 actcaaactt ttaatgagag tttgatcctg gctcaggacg aacgctggcg gcgtgcctaa
      181 tacatgcaag tagaacgctg aaggaggagc ttgcttctct ggatgagttg cgaacgggtg
      241 agtaacgcgt aggtaacctg cctggtagcg ggggataact attggaaacg atagctaata
      301 ccgcataaga gtagatgttg catgacattt gcttaaaagg tgcacttgca tcactaccag
      361 atggacctgc gttgtattag ctagttggtg gggtaacggc tcaccaaggc gacgatacat
      421 agccgacctg agagggtgat cggccacact gggactgaga cacggcccag actcctacgg
      481 gaggcagcag tagggaatct tcggcaatgg acggaagtct gaccgagcaa cgccgcgtga
      541 gtgaagaagg ttttcggatc gtaaagctct gttgtaagag aagaacgagt gtgagagtgg
      601 aaagttcaca ctgtgacggt atcttaccag aaagggacgg ctaactacgt gccagcagcc
      661 gcggtaatac gtaggtcccg agcgttgtcc ggatttattg ggcgtaaagc gagcgcaggc
      721 ggttagataa gtctgaagtt aaaggctgtg gcttaaccat agtaggcttt ggaaactgtt
      781 taacttgagt gcaagagggg agagtggaat tccatgtgta gcggtgaaat gcgtagatat
      841 atggaggaac accggtggcg aaagcggctc tctggcttgt aactgacgct gaggctcgaa
      901 agcgtgggga gcaaacagga ttagataccc tggtagtcca cgctgtaaac gatgagtgct
      961 aggtgttaga ccctttccgg ggtttagtgc cgtagctaac gcattaagca ctccgcctgg
     1021 ggagtacgac cgcaaggttg aaactcaaag gaattgacgg gggcccgcac aagcggtgga
     1081 gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca tccctctgac
     1141 gactctagag atagagtttt ccttcgggac agaggtgaca ggtggtgcat ggttgtcgtc
     1201 agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaacccct attgttagtt
     1261 gccatcattt agttgggcac tctagcgaga ctgccggtaa taaaccggag gaaggtgggg
     1321 atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgctac aatggctggt
     1381 acaacgagtc gcaagccggt gacggcaagc taatctctta aagccagtct cagttcggat
     1441 tgtaggctgc aactcgccta catgaagtcg gaatcgctag taatcgcgga tcagcacgcc
     1501 gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag agtttgtaac
     1561 acccgaagtc ggtgaggtaa ccgtaaggag ccagccgcct aaggtgggat agatgattgg
     1621 ggtgaagtcg taacaaggta gccgtatcgg aaggtgcggc tggatcacct cctttctaag
     1681 gataaggaac tgcgcattgg tcttgtttag tcttgagagg tcttgtgggg ccttagctca
     1741 gctgggagag cgcctgcttt gcacgcagga ggtcagcggt tcgatcccgc taggctccat
     1801 tggtgagaga tcaccaagta atgcacattg aaaattgaat atctatatca aatagtaaca
     1861 agaaaataaa ccgaaaacgc tgtagtatta ataagagttt atgactgaaa ggtcagaaaa
     1921 taaggttaag ttaataaggg cgcacggtgg atgccttggc actaggagcc gacgaaggac
     1981 gtgacaaacg acgatatgcc ttgggtagct gtaagtaagc gatgatccat ggatttccga
     2041 atgggggaac ccaacaggta atacctgtta cccacatctg ttaaggatgt gaggaggaag
     2101 acgcagtgaa ctgaaacatc taagtagctg caggaagaga aagcaaaagc gattgcctta
     2161 gtagcggcga gcgaaacggc agaagggcaa accgaagagt ttactcttcg gggttgtagg
     2221 actgcaatgt ggactcaaag attatagaag aatgatttgg gaagatcagc caaagagagt
     2281 aatagcctcg tatttaaaat agtctttgta cctagcagta tcctgagtac ggcgggacac
     2341 gtgaaatccc gtcggaatct gggaggacca tctcccaacc ctaaatactc cctagtgacc
     2401 gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc ccgggagggg agtgaaatag
     2461 aacctgaaac cgtgtgccta caacaagttc gagcccgtta atgggtgaga gcgtgccttt
     2521 tgtagaatga accggcgagt tacgttatga tgcgaggtta agttgaagag acggagccgt
     2581 agggaaaccg agtctgaata gggcgcctta gtatcatgac gtagacccga aaccatgtga
     2641 cctacccatg agcaggttga aggtgcggta agacgcactg gaggaccgaa ccagggcacg
     2701 ttgaaaagtg cttggatgac ttgtgggtag cggagaaatt ccaaacgaac ttggagatag
     2761 ctggttctct ccgaaatagc tttagggcta gcgtcgacat tagagattct tggaggtaga
     2821 gcactgtttg ggtgaggggt ccatcccgga ttaccaatct cagataaact ccgaatgcca
     2881 atgaattatg gtcggcagtc agactgcgag tgctaagatc cgtagtcgaa agggaaacag
     2941 cccagaccac cagctaaggt cccaaaataa ttgttaagtg gaaaaggatg tggggttgca
     3001 cagacaacta ggatgttagc ttagaagcag ctattcattc aaagagtgcg taatagctca
     3061 ctagtcgagt gaccctgcgc cgaaaatgta ccggggctaa aacaatttac cgaagctgtg
     3121 gataccttta taggtatggt aggagagcgt tctatgtgtg atgaaggtat accgtgagga
     3181 gtgctggaac gcatagaagt gagaatgccg gtatgagtag cgaaagacag gtgagaatcc
     3241 tgtccaccgt aagactaagg tttccagggg aaggctcgtc cgccctgggt tagtcgggac
     3301 ctaaggagag accgaaaggt gtatccgatg gacaacaggt tgatattcct gtactagagt
     3361 atgtagtgat ggagggacgc agtaggctaa ctaaagcaga cgattggaag agtctgtcta
     3421 agcagtgagg tgtgaattga gtcaaatgct taattctata acattgagct gtgatgggga
     3481 gcgaagttta gtagcgaagt tagtgacgtc acactgccaa gaaaagcttc tagcgtttaa
     3541 acatactcta cccgtaccgc aaaccgacac aggtagtcga ggcgagtagc ctcaggtgag
     3601 cgagagaact ctcgttaagg aactcggcaa aatgaccccg taacttcggg agaaggggtg
     3661 ctgactttaa gtcagccgca gtgaataggc ccaagcaact gtttatcaaa aacacagctc
     3721 tctgctaaat cgtaagatga tgtatagggg gtgacgcctg cccggtgctg gaag
//
LOCUS       DAVBNO010000096         3580 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVBNO010000096 DAVBNO010000000
VERSION     DAVBNO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.096"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(180..791)
                     /gene="rpsD"
                     /locus_tag="VQ846_002013"
     CDS             complement(180..791)
                     /gene="rpsD"
                     /locus_tag="VQ846_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET3076529.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(986..2038)
                     /locus_tag="VQ846_002014"
     CDS             complement(986..2038)
                     /locus_tag="VQ846_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET3076530.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2035..2733)
                     /locus_tag="VQ846_002015"
     CDS             complement(2035..2733)
                     /locus_tag="VQ846_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET3076531.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2900..>3580)
                     /locus_tag="VQ846_002016"
     CDS             complement(2900..>3580)
                     /locus_tag="VQ846_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HET3076532.1"
                     /translation="PFTAGVNHVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPA
                     LSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKP
                     DLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGLLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT          973 a          806 c          705 g         1096 t
ORIGIN      
        1 attataccac attgtgtact atttttggtt cattttacta tatcactttg gactatatcg
       61 gtctagttat gtctcaacga ttatagctgg atactaagag aaatcaaaaa agcactaagg
      121 gagaacgtcc caaagtgctt aatatcaagg cctcaaagcc tgtcttattc aataaaatat
      181 tacaacatct tgttgtagaa ttcaacgaca agtgcttcgt tgatttctgg gttgatttcg
      241 tcgcgttctg gcaagcgagt caatgaacct tccaactttt cagcgtcgaa tgatacgaat
      301 gctggacgtc caagagtagc ttctactgct tcaaggattg ctggaacttt caatgatttt
      361 tcacgaactg agatcacttg acctggagtt acgcggtatg atgggatatc aacgcgtttc
      421 ccgtcaacaa ggatgtgacc gtggtttacg aattgacgag cttgacgacg agtagtcgcg
      481 agaccaagac ggtaaacaac attatccaaa cgacgttcca aaagaagcat aaagttgaaa
      541 cctaggattc cacctttgat ttttgtagct tgtacgaaca agttacggaa ttgtttttca
      601 cctacaccgt aagtgaaacg aagtttttgt ttttcagcca attgcaaacc gtattctgac
      661 aatttagaac ggttgtttgg tccgtgttgt cctggtacgt agttacgacg tgccaattct
      721 ttacctgtac ctgtaagtga aaggccaagg cgacgagctt gtttccaaga tggtcctgta
      781 taacgtgaca tatgaatgtc ctcctgatat aaataatatt tcggtggaaa tagtcactta
      841 gaaagccctg attcgtgcag atgcccttcg cctaaacagc caaggttact tatcataaga
      901 cacctgttga cgagcttcat gctttcctgc tgatatttca cacaaaatcc attctaccat
      961 gaatagctgg atttgtaaag gggttttagg ctttattttc actaccagag aggttgagaa
     1021 cgagggtaaa ggtacttcct agaccgtact ggctgctgac tgtgatttcc ccacccaatt
     1081 gatgggccaa ttcacgcgca atcgcaagtc ctaatccatg accacctgtc ttcatgttac
     1141 gcgaagtttc gacacgataa aggcgtttga aaatattttc caaatcctct ggggcaatgc
     1201 cctgcccttc atcggtcaca ctgattgaaa gctggtcctt ctccagctta gccaccactt
     1261 ccagcttggt tcctggagca gaatatttaa aagcgttatc gaccagattc accaagatac
     1321 gagaaagctt agcataatct ccctcaatcc gggcagactc tgggattacc tgcaagtgga
     1381 catctcttct ctcctgctca atcaaaaact gaaattcact catgcactca attaagagct
     1441 tgtccagaaa aatactgtct ttactggtag tttccacctg atttctagct gtgtttaggg
     1501 tcaaaaaatt caactcctca accagtttat tgagcctctc cgtctggcgt ccaatggttg
     1561 ctagataatg agcttgctcc gactccttga taatcccatc caaaatccct tctaccgtcg
     1621 cttggatcga agtgatagga gtcttaatat catgcgacaa ctgagcaatc atcaagccct
     1681 tttctcgttc gctttcttcc aaggaatcaa agcttacctg caaatcatgg gacatctcat
     1741 taaaagtttg ccctaattgc tgaaattcta caggaccttg aacctccaaa tttgaaggaa
     1801 aatccttggc cgctacccgc ttggcatgct ccttgagttt gcccaacgac gtaaagactg
     1861 gcaataggag aaagagacta atcccagcac cgacaaggct ggcaacgatg gtcatcccaa
     1921 gcaaaaagta aatctcgcct ttcgcaatca gcattttttg aacagcccaa aaaacaacca
     1981 aaatggttaa gagggttgaa ataatatatc caaccaaaat ataacttttt agtttcatgt
     2041 ttgtcctctc ggtttctcta tcttatatcc caacccccaa actgtcttaa tagtgggcgt
     2101 ttggtcacta ctatattttg ccagctcctg tcgaagagca tggatatgca cattcaaggt
     2161 attggtgtca tccacgtagt cttctttcca gatcttttca tagaggtctg tcttggagaa
     2221 aactcgctct ggattgctag ctaaaatcca cagcaattca aatgatttaa cagttaaatc
     2281 cagcatttct tctcctattt gaacttcatg actactatga ttcattttta gattgccaag
     2341 ggaaatcagc tctgtttcgc ccccacgatg aaggcggcgc aaaatattgt ggacacgcaa
     2401 aaccagttca cgtgggctaa aaggcttagc aataaaatca tctgctccca agctcaggcc
     2461 gtaaatcttg tcctgttcac tggtcttagc agtaataaat aggaaaggct gctctggtga
     2521 taagtattga acctcactga ttaaatcata accatccatc cgaggcatca tgacatctgt
     2581 gataatcaaa tcaatcggtt ttttcttgaa cagctctagc gcttccagtc catcatgggc
     2641 taccaagacc tgataacctg cctgaattaa gtatctctga tgaatatctg tgatttctac
     2701 ctcgtcgtca acgagtaaaa ttgtctttcc catctgtctc tcctttgata aaaacagtgc
     2761 tatactgctt taagtataac actattttta ttgttttacg atgattttaa tctgctattg
     2821 aaagaaagtc ctgattgctg attgagttgt taaactaatc ttagtcagcc cactatccat
     2881 catttatttc cttagctgat tagttttctt cttttttgtt tttcaaacct agaccaccga
     2941 gtaaacctgc aagcgcaagc ccaagtaaac caatacttgc cattgatgtt tgagtctcac
     3001 cagtatttgg tagcatagct ttatcctctg acatcatcgt atcagacatc ttgttagcag
     3061 aagcagccat gttttcacct gccatcgtgt tggtagaact tgtcatggtg tcagcaggca
     3121 tgctatctgt agtacctgta gcatgattgt gattcatcgg agtcacgcca gaaccagagt
     3181 tagaaggtga taatgaacca tttgctgtgt ctgatgtttc tttaacattt atcttaatag
     3241 tgactttttt agttgctacg atgttgtcca agtctggttt accgtctttg ttaccataga
     3301 cattgactgt agcgctgtaa gtttgagtgc cgtttgctcg gaactggtca atgagcgctt
     3361 gtttttcttt gccagctaca tttccgtcca aggctacttg atagaagtat tgacctttgg
     3421 tcttcacgtt ttcgcctagt ggagataggg ctgggttttt agcgtcgccg ttatctgacc
     3481 atggtgcctt gtcagatgcc ttgagcaaga gacgagtcaa catgccatca cctgcgaaga
     3541 gttcgtatgg aatcacatgg ttgacacctg ctgtgaatgg
//
LOCUS       DAVBNO010000097         3075 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVBNO010000097 DAVBNO010000000
VERSION     DAVBNO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.097"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..249
                     /locus_tag="VQ846_002017"
     CDS             <1..249
                     /locus_tag="VQ846_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET3076533.1"
                     /translation="YQGLMKIYPQAQIPRKSSKLKPLIAEDKAYNHALSKDRSKVENI
                     LAKVKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            330..1046
                     /locus_tag="VQ846_002018"
     CDS             330..1046
                     /locus_tag="VQ846_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET3076534.1"
                     /translation="MKKRYLVVTALLALSLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            1355..1780
                     /gene="rplK"
                     /locus_tag="VQ846_002019"
     CDS             1355..1780
                     /gene="rplK"
                     /locus_tag="VQ846_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET3076535.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            1988..2677
                     /gene="rplA"
                     /locus_tag="VQ846_002020"
     CDS             1988..2677
                     /gene="rplA"
                     /locus_tag="VQ846_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET3076536.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
BASE COUNT         1037 a          581 c          681 g          776 t
ORIGIN      
        1 tatcaagggc tcatgaagat atatcctcaa gcacaaattc cacgtaaatc cagcaaactc
       61 aagccgctaa tagctgaaga taaagcctat aaccatgcgc tatctaagga tagaagcaag
      121 gttgagaaca tccttgccaa agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat
      181 catcgtaaac gcttcggatt acgaatgaat ttgattgctg gtattatcaa tcatgaacta
      241 ggattctagt tttgcaggaa gtctattaaa agattagtca aacgtttatc atcagaagta
      301 gataaactat tagaaataaa ggagtagaaa tgaagaaaag ataccttgtc gtgacagcct
      361 tgctagcctt gagtctagca gcttgttcac aagaaaaagc aaaaaatgaa gatggcacag
      421 ctaagacaga acagacagcc aaagctgatg ggacagtcgg cagtaagtct caaggggctg
      481 cccagaaaaa agcagaagtg gtcaataaag gtgattacta cagcatccaa ggaaaatacg
      541 acgaaatcat cgtagccaat aaacattatc ctttgtcgaa agactataat ccgggggaaa
      601 atccaacagc caaggctgag ttggtcaaac tcatcaaagc gatgcaagag gcaggtttcc
      661 ctattagtga tcattacagt ggttttagaa gttatgaaac tcagaccaag ctctatcaag
      721 attatgtcaa ccaagatgga aaggcagcag ctgaccgtta ctctgcccgt cctggctata
      781 gcgaacacca gacaggcttg gcctttgatg tgattgggac tgatggtgat ttggtgacag
      841 aagaaaaagc agcccaatgg ctcttggatc atgcagctga ttatggcttt gttgtccgtt
      901 atctcaaagg caaggaaaag gaaacaggct atatggctga ggaatggcac ctgcgttatg
      961 taggaaaaga agctaaagaa attgctgcaa gtggtctcag tttggaagaa tactatggct
     1021 ttgaaggcgg agactacgtc gattaatact cttcgaaaat ctcttcaaac cacgtcagcg
     1081 tcgccttacc tactgactgc gtcggttcta ttcacaacct caaaacagtg ttttgagctg
     1141 acttcgtcag ttttatctgc aatctcaaag ctgtactttg agcagcctac agctagcttc
     1201 ctagtttgct ctttgatttt cattgagtat aaaaaataaa cttttctctt gcaattccag
     1261 ataaatagtg tataatggat gggtatgtga aaaacatact tgtgggaggt aaaaatctct
     1321 aattaccgcc aaaaccacaa aggaggattt aaaaatggct aaaaaagtcg aaaaacttgt
     1381 aaaattgcaa atccctgctg gtaaagctac accagctcca ccggttggac ctgctcttgg
     1441 tcaagctggt atcaacatca tgggattcac aaaagagttc aacgctcgta cagctgacca
     1501 agctggtatg atcattccag ttgttatctc agtttacgaa gataaatcat ttactttcgt
     1561 tacgaaaaca ccaccagctg ctgttctttt gaaaaaagct gcaggtgttg aaaaaggatc
     1621 aggtacacct aataaaacta aagttgctac agttactcgt gcacaagtac aagaaattgc
     1681 agaaactaag atgccagatt tgaacgcagc aaacgtagag tctgcaatgc gtatgatcga
     1741 aggtactgct cgttctatgg gattcactgt tgttgactaa tcaataacac agtagaaaat
     1801 ctcacagcag tctattagtt gcttttcata ctaggcaagt gactgaggct tgtacttggg
     1861 tacagcaagg gagcttaagg ccgtaggaag agaaaaatag tagactgaaa acccgcaaga
     1921 cttcatcatt tcgagaagtg acgtgggaga tgaaaatcga ttgaaccact tacaaggaga
     1981 atagaaaatg gctaaaaaaa gcaaacaact tcgtgctgct cttgagaaaa tcgacagcac
     2041 aaaagcatac agcgtagaag aagctgtagc acttgcaaaa gaaactaact ttgcaaaatt
     2101 tgacgcaact gtagaagttg cttacaactt gaacatcgac gttaaaaaag ctgaccaaca
     2161 aatccgtgga gcaatggtat tgccaaacgg tactggtaaa acttcacgtg ttcttgtttt
     2221 cgcacgtggt gcaaaagctg aagaagcaaa agctgctggt gcagactttg ttggtgaaga
     2281 tgaccttgtt gctaaaatca acgacggttg gttggatttc gacgtagtta tcgctacacc
     2341 tgatatgatg gctcttgttg gacgtcttgg acgtgtcctt ggaccacgta acttgatgcc
     2401 aaaccctaaa actggtactg taacaatgga tgttgctaaa gcagttgaag agtctaaagg
     2461 tggtaaaatc acttaccgtg ctgaccgtgc aggtaacgtt caagcaatca tcggtaaagt
     2521 atcatttgaa gctgaaaaat tggttgaaaa ctttaaagct ttcaacgaaa caatccaaaa
     2581 agcaaaacca gctacagcta aaggaactta cgtaacaaac ttgactatca caactactca
     2641 aggtgttggt atcaaagttg acgtaaactc actttaatca gtagttaaag taatgtaaaa
     2701 aagttgaaga cgctatgtct caactttttt tgatatacga cgggcatgtc gtatatctga
     2761 ggtgtaaatc ctcggtgggc acctactact ggtgaaccca atagcgattc ccaagtctga
     2821 ctatcgtgag gtagcggatt aaaacggtct ggggatagac cgttttaagt ctgacgctgg
     2881 aaataagaat cgtcagagga agggatagcg aaatcgtggc tctacgaaca ggaacgtgat
     2941 aataaggcct atatagcgga taaggtatct ggctggggcg accactatca attatagtaa
     3001 aatgaaataa gagtaggacg aatcgatcag ggcagccaaa tcgatttcta acaatgtttt
     3061 agaagaagag atgta
//
LOCUS       DAVBNO010000098         2901 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVBNO010000098 DAVBNO010000000
VERSION     DAVBNO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.098"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(64..1005)
                     /locus_tag="VQ846_002021"
     CDS             complement(64..1005)
                     /locus_tag="VQ846_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET3076537.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(983..2746)
                     /gene="aspS"
                     /locus_tag="VQ846_002022"
     CDS             complement(983..2746)
                     /gene="aspS"
                     /locus_tag="VQ846_002022"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET3076538.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVVNATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EGTAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT          845 a          686 c          577 g          793 t
ORIGIN      
        1 aaccatatat acggtaaggc gacgctgacg tggtttgaag agattttcga agagtattaa
       61 tcactaatta tctatctcaa caaatcttcc tagaatatga acattttccg agacagagac
      121 aaaggagctt ggatccactt gtgtcataat ctgtttaaat tcattaaact ctgcacgtgt
      181 aatgacagtg attaaaactg cctttctctc gtgattatag gttccttctg catcatggat
      241 catggttgct ccgcggtgca attttttatg gattttttca attaccttct ctggatgatt
      301 tgtcacaatc atggcctgca tacgcttttg cttagtaaag actgcgtctg tcacacgact
      361 agagacaaag atggtaatca tagaataaag agcgtatttc caaccaaagg tcaaacctgc
      421 tatcagcatg atagttccat ttaccaagaa agaaatacta ccgacattct tacccgtttt
      481 cttgcgaata gtcaggctga cgatatccgt cccaccactg gagatattgt ttcgaagagc
      541 aaaaccaatc cccaaaccca taacaacacc cccaaaaagg gcattgataa tgggatcctc
      601 cgtcaaggtt gccacaggga caaactggat aaagaaggaa ctcatagata ccgtgataaa
      661 ggtaaagacg gtgaacttat ggccaatctg ataccaagct aagaccatca aagggaagtt
      721 aatggcgtaa aagcttagcg aaatcggaat atgaaaacca aaccagtgat tactcaaggc
      781 agagataatc tgtgccagac ctgttgcacc actcgaatac acatgccctg gttggaaaaa
      841 gaaattaact gctactgctg ataaaaaacc atagaccaga gaggccgaaa tcttctcatc
      901 atacttttct cgagagatac tttgtaagac acgtaaaatt tttatctgat aagcaaagcg
      961 gcgcagataa tagcgccacc gcttaattcg ttttgtttgt ttcatcttct tctacttgta
     1021 agctgagttc ctctagttgt ttgagagcga ctgttgatgg agcttgtgtc attgggtcag
     1081 ttgccttgtt gttcttagga aaggcaatga cttcacggat attttcttct ccagcaagca
     1141 acatgacaaa acggtcaagc ccgatagcca aaccaccgtg tggtgggaaa ccatagtcca
     1201 tggcttcaag aaggaaacca aactggtcat tggcttcttc agttgagaaa ccaagagcct
     1261 tgaacatgcg ttcttgaagg tctttttggt tgatacgaag gctaccacca ccaagctcat
     1321 aaccgttcaa gacgatatcg taagcaatgg cacgaacctt agccaaatca ccttctaatt
     1381 cgtgagcagt cccttcctgt ggaagtgtga atggatggtg ggcgctcatg tagcggcctt
     1441 cttcttcaga ccattcaaac atcggccagt caaccaccca aaggaagttg aacttatcat
     1501 tatcaatcaa gccaagctct ttagcaatac gtccacgaag ggcacccagt gttgcattaa
     1561 ccacttcaag cgtatccgcc acaaagagaa ccaagtcctt atcttcaaga gcaagcgctg
     1621 ttgtcaattc ttcttggata ccagtcaaga acttggcaac tggtccgttt aattctccat
     1681 caaccacctt gacccaagca agacctttgg caccatactg tttggctact tccgtcatct
     1741 tgtcgatgtc tttacgtgaa tagttgtccg cagctccttt gaccacaatc gcttttacag
     1801 caggtgcttc tgaaaagact ttaaagtcta cacctttgac cacttctgtc aagtcctgaa
     1861 gcaacatgtc aaaacgagta tctggcttgt cagaaccgta aagagccata gcatcatcgt
     1921 atttcatacg agggaacggt agcgttactt cgatgccttt tgtttccttc atcacgcgcg
     1981 cgatcaagcc ttctgtaata tcttggattt ctttctcagt aaggaaggac gtttccaagt
     2041 cgacctgagt aaattcaggc tggcggtctc cacgcaagtc ctcgtcacgg aaacatttaa
     2101 cgatttggta gtaacggtca aaaccagcat tcatcaagag ctgtttcgtg atttgtggac
     2161 tttgaggaag agcgtaaaaa tgccccttat taacacgaga cggcactaaa taatcacgcg
     2221 ccccttcagg cgttgacttg gaaaggaatg gtgtctccac gtcgataaac tccaactcat
     2281 ccaaatagtt gcggatagag tgggtcacct tggcacgaag tttaagattt tccaacattt
     2341 ctggacgacg aaggtcaagg taacggtaac gcaaacgtgt atcgtcattt gcctcaatgc
     2401 catccttaat ctcaaatggt gttgtcttag ctgtgttaag cacaataaga gctgtcacgt
     2461 ttaactcaac cgcaccagtt ggcaacttat cattggcttg ctcacgcgca gcgacctgac
     2521 cagtcacctc aataacaaat tcgctacgaa ggctttcagc tgttgccata acctctgcag
     2581 atactttttc agggttgata accaactgca tgattccttc acggtcacga agatcgataa
     2641 agatcaaacc accaaggtca cgacgacggc caacccatcc tttcaaggtt atttcttgtc
     2701 cgatgtgttc ctcacgaaca cgaccagcat acatactacg tttcattatt tctctcctct
     2761 tttattctgt tactatttta ccataaaagc gcaggctctt catgaaaatc atcagaaaag
     2821 tttgccagtc tttaaaagtc aggtgaaagc cctaaaaatt agcgctaatg ctcttcgaaa
     2881 atctcttcaa accacgtcag c
//
LOCUS       DAVBNO010000099         2658 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVBNO010000099 DAVBNO010000000
VERSION     DAVBNO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.099"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            448..2553
                     /locus_tag="VQ846_002023"
     CDS             448..2553
                     /locus_tag="VQ846_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET3076539.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTSEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
BASE COUNT          850 a          480 c          614 g          714 t
ORIGIN      
        1 tcctgatcga tttgtcctgt tcttatttca ttttaatata tagttttgag atggttgata
       61 gaactgacga agtcagctca aaacatggtt ttgaggttgt agataaggag aagctgatgt
      121 ggtttgaaga ggttttcgaa gagtgtaaaa aaatattggt cagttaaagc caaaactctt
      181 gaccttttct gaccaatgta gtatattatg aacgtaaggt aaatgaaagc cttactagaa
      241 cacttattta aagtaaaata gaaagaggtg ggtctatgtt tcatctagaa atcttcagaa
      301 gtaaagatgg tctactcctg cttgaaaaag aaaaaccaga aatagtacgt agggtagcgg
      361 tttaactagt ctaaattttt taaaacaatg gtcaaagata gtcaatatca gtaatcataa
      421 ctaagtaaac aaaaagaggt aaagaatatg aataacaact ttaataattt taataacatg
      481 gatgatttat ttaaccaatt gatgggtggt atgcgaggat atagttctga aaatcgccgt
      541 tacttaatta atggacgcga agtcacatct gaggaatttg ctcactatcg tgcgactggt
      601 caattaccag gaaatgcaga aactgatgtg caaatgccac aacaggcatc aggtatgaaa
      661 caagacggtg tccttgcaaa actaggtcga aacttgacag cagaagcgcg tgagggtaag
      721 ttggatcctg ttatcggacg aaacaaggaa attcaagaaa catctgaaat cctctcacgc
      781 cgcaccaaga acaatcctgt tttggtcgga gatgcaggtg ttggtaagac agcagttgtc
      841 gaaggtctag cgcaagccat tgtgaacgga gatgttcctg ctgctatcaa gaacaaggaa
      901 attatttcta ttgatatctc aggtcttgag gctggtactc aataccgtgg tagctttgaa
      961 gaaaatgtcc aaaacttagt caatgaagtg aaagaagcag ggaatattat cctcttcttt
     1021 gatgaaattc accaaattct tggtgctggt agcactggtg gagacagtgg ttctaaagga
     1081 cttgcggata ttctcaagcc agctctctct cgtggagaat tgacagtgat tggggcaaca
     1141 actcaagacg aataccgtaa caccatcttg aagaatgctg ctcttgctcg tcgtttcaac
     1201 gaagtgaagg tcaatgctcc ttcagcagag aatactttta aaattcttca aggcattcgt
     1261 gacctctatc aacaacatca caatgtcatc ttgccagacg aagtcttgaa agcagcggtg
     1321 gattattctg ttcaatacat tcctcaacgt agcttgccag ataaggctat tgaccttgtc
     1381 gatgtaacag ctgctcactt ggcggctcaa catccagtaa cagatgtgca tgctgttgaa
     1441 cgagaaatcg aaacggaaaa agacaagcaa gaaaaagcag ttgaagcaga agattttgaa
     1501 gcagctctaa actataaaac gcgcattgca gaattggaaa ggaaaatcga aaaccacaca
     1561 gaagatatga aagtgactgc aagtgtcaac gatgtggctg aatctgtgga acgaatgaca
     1621 ggtatcccag tatcgcaaat gggagcttca gatatcgaac gtttgaaaga tatggctcat
     1681 cgcttgcaag acaaggtgat tggtcaagat aaggccgtag aagctgtagc tcgtgctatc
     1741 cgtcgtaacc gtgctggttt tgatgaagga aatcgcccaa tcggcagctt cctctttgta
     1801 gggtctactg gggttggtaa gacggagctt gctaagcaat tggcactcga tatgtttgga
     1861 acccaggatg cgattatccg tttggatatg tctgaataca gtgaccgcac agctgtttct
     1921 aagctaattg gtacaacagc aggctatgtg ggttatgatg acaatagcaa taccttaaca
     1981 gaacgtgttc gtcgcaatcc atactctatc attctcttgg atgaaattga aaaggctgac
     2041 cctcaagtta ttacccttct cctccaagtt ctagatgatg gtcgtttgac agatggtcaa
     2101 ggaaatacag taaacttcaa gaacactgtc attattgcga cctcaaatgc tggatttggc
     2161 tatgaagcca acttgacaga agatgcggat aaaccagaat tgatggaccg tttgaaaccc
     2221 ttcttccgtc cagaattcct caaccgcttt aatgcagtca tcgagttctc acacttgact
     2281 aaggaagacc tttctaagat tgtagatttg atgttggctg aagttaacca aaccttggct
     2341 aagaaagaca ttgacttggt agtcagtcaa gcggctaaag attatatcac agaagaaggt
     2401 tacgacgaag tcatgggggt tcgtcctctc cgtcgagtgg ttgaacaaga aattcgtgat
     2461 aaggtgacag acttccactt ggatcattta gatgctaaac atctggaagc agatatggaa
     2521 gatggcgttt tggttattcg tgagaaagcc taagacagaa ttttgaggat aaaaaagaag
     2581 gagccagctg aaaaaaactg gttcctttct aggtacgacg ggcatgtcgt gtagtaggtt
     2641 gaaactagaa tagtacac
//
LOCUS       DAVBNO010000100         2535 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVBNO010000100 DAVBNO010000000
VERSION     DAVBNO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.100"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..559
                     /locus_tag="VQ846_002024"
     CDS             <1..559
                     /locus_tag="VQ846_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET3076540.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYAWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
     gene            616..1413
                     /gene="psrA"
                     /locus_tag="VQ846_002025"
     CDS             616..1413
                     /gene="psrA"
                     /locus_tag="VQ846_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HET3076541.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
     gene            1424..2029
                     /locus_tag="VQ846_002026"
     CDS             1424..2029
                     /locus_tag="VQ846_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET3076542.1"
                     /translation="MVKLTTCYFCDYGSYYGNIPMNWVVIKIKDIFSMNTGLSYKKGD
                     LSINNKGVRIIRGGNIKPLEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHI
                     GKFARIDKDYDGVVAGGFIFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQA
                     LYNIPKTTLSELLIPLAPFEEQELITQKVEKLFEKVNQLWK"
     gene            complement(1977..>2535)
                     /locus_tag="VQ846_002027"
     CDS             complement(1977..>2535)
                     /locus_tag="VQ846_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET3076543.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIISKVDLLFQKVSQLFE"
BASE COUNT          864 a          363 c          439 g          869 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagggat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgc
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcggggg ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      721 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      781 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
      841 ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctgat
      901 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
      961 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1021 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1081 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg tcgaagtggt
     1141 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1201 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1261 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1321 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1381 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtggtca aactgactac
     1441 ctgctatttt tgtgattatg gctcttatta tgggaatata cctatgaatt gggttgttat
     1501 aaaaataaaa gatatttttt caatgaatac aggtctttct tacaagaagg gcgatttaag
     1561 cattaataat aaaggtgtta gaattatacg tggtggtaat attaagcctt tagaattttc
     1621 tctgttggat aatgattact acattgatac acaattcatc tcctctgaac aagtttattt
     1681 aaaacataat cagctaataa cacctgtatc aacctcttta gaacatattg gaaagtttgc
     1741 aagaatcgat aaagactatg atggtgttgt ggctggtgga tttattttcc aattaacacc
     1801 attcgaaagt tcagagatta tttcaaaatt tctattattt aacttgtctt ctccgttatt
     1861 ttataaacaa ttgaaagcaa taactaaact atcaggtcaa gctttatata atattcctaa
     1921 aactacactg agcgagctat taattccgtt agctcctttt gaggaacagg aacttattac
     1981 tcaaaaagtt gagaaacttt ttgaaaaagt aaatcaactt tggaaatgat tcttttcatc
     2041 tcttcatgat tagaaatagg gattaataat tcggagatac tggtactatt taatgttttc
     2101 cctttgatag catcttttga atcacctaaa gtagagataa gtggcaaaaa tatcattaag
     2161 taatctctga taatattttc tttattagca taggggaata tcgatataat ggcttcatta
     2221 tgagtggcag gaatatccaa tatggcaact tttccaatag ataatttaaa actcattaat
     2281 aaagttcctt taggtgaaat gtctattttc tttgatttta atgctaattt agaaatagat
     2341 tctctcgtat tagttacata accagatata ggcatatctg atatagatac ccaaggtatt
     2401 tcagttcccc aaaaagtagc ttcactgcgt ggaggagttt ttcctattct gaagttaact
     2461 aggctagcaa atttaatata tctccatgct tctgggattt catatatagg ataagaggtt
     2521 gtttcgtctt tgttc
//
LOCUS       DAVBNO010000101         2481 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVBNO010000101 DAVBNO010000000
VERSION     DAVBNO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.101"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(173..838)
                     /locus_tag="VQ846_002028"
     CDS             complement(173..838)
                     /locus_tag="VQ846_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET3076544.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            complement(852..2231)
                     /locus_tag="VQ846_002029"
     CDS             complement(852..2231)
                     /locus_tag="VQ846_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET3076545.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRFIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
BASE COUNT          813 a          565 c          463 g          640 t
ORIGIN      
        1 cccgcagcta gtttcctagt ttgatctttg attttcattg agtattactc tctcttgtca
       61 cttccttcta ttttaccata aagtccagcc tttgaagaac ttttactaga agacaagggg
      121 cttctgtctc tatttgccat cttgggcatc aaaaaagagg ggtcatccct ctttacgaat
      181 tcaatgctac tagggtatcc aaatactggt tgttgatgac tgccaaaata taggtatctg
      241 ctttcaagag gtcatctggt ccaaattcaa catccaatgg ggaattttcc tgctctcgga
      301 aacccaaaat attcagattg tatttgccac ggaggtctaa tttactcaga ctttgacctg
      361 cccaagactg aggaattttc atctccacga tagacacatt tttatccaac tgaaagacat
      421 caacactatt atggaaaaga atggtctgtg ctagagactg ccccatttca tactctggcg
      481 agataaccga gtcagctcca atcttttcta gcactttctt agcggtctga cttttgacct
      541 tagcaataac agtcggtacc cccaaactct tacagtgcat aaccgcaagc acactcgact
      601 ccagattttc acctgtcgcg actacaacgg tatcgcaggt atcaatccct gctgatctca
      661 ataattcttc atctgtgatg tcaccaatca ctccacgcgc caaaactggc tcaaactgat
      721 tgatgcgctc tgcgtggtca tcaatagcga taatattcat atcctgcttg gctagggcag
      781 ctaggacact gctcccaaaa attcccaagc ccaaaattcc aatcgtacga tctgacatgc
      841 tctttccttt cttaaccaat actaatatct gctttcatat agtgaatcat atctttcttt
      901 tctggatggt aatctgccaa gctaacaaac aaggtcaagg gaccaattcg tcccataaac
      961 ataagtggca tgataacact gagagccaat ttcccaaggt caggagtcag atttgccgtt
     1021 acaccaactg tactaagagc tgaaatggtt tcaaatatga ggtggataaa gggaggattg
     1081 cctttggctg ttatccctag cagaatcaat cctatcaaga agctcatcaa aaagataata
     1141 aagacactaa aggatttttg aaccgttcgc ggcgcaatcg ttcgtctcgc aacattggca
     1201 tgaggcaagc ctagaagctc acttcgtgca aagaccaaga ggacaaaaaa tgtcgtaatc
     1261 ttgagtcccc cagctgttcc tccaggtgcc ccacctagaa acatctgtaa gatataaatc
     1321 aaaagagtca caggatgagc ctgagtataa tctatcgtag aaaagccagc tgttcgcatc
     1381 gtcactgttt gaaaaaagct aactaaaacc ttatcggcaa cagggagatt gccaatcgtt
     1441 ccagcattgt tccactcaag aaagagagta gttgccgttc caaataacaa caaacctata
     1501 gtcaacaata gtacaagctt cgtatgaaag tgcagacgtc cttttttctt tcttcctaca
     1561 tgaccagcca aatcaaacca gaccataaaa ccaaggccgc ctgtaataat caagcctgca
     1621 ataaccagat tgaccagtaa atcggtctga aaagcaaata aactggtgct ccctaaatta
     1681 tcaaaaccgg cattacagaa ggctgagatc gctagaaaaa tggaactaaa aagaccacgt
     1741 ccccagccaa gttgaggaat aaagcgaaaa ctaagcaaaa tagctcccaa gctctcaacc
     1801 aaaaaggtcg tgagaaaaat agaatagaca aactttctca aagatcgagt ttctccataa
     1861 ctaaaactat cctgaatagt tgcacggcta cgaagactaa gcttttgctt gctctggata
     1921 tagaaaaccc caataaaggt catgagccct agaccaccga tctgaatcaa gagcaaacag
     1981 attatttggc cccagatatt ataggtgtga gctactggaa gggttgagag acctgtcaca
     2041 cagactgcag agacagcagt gaaaagatga tcaaaataag tcgctcgtga gctttcaact
     2101 tggacaaagg gcaagctcaa aagaagagag cctagtaaaa tgaccaaggc aaaactcaaa
     2161 aaaatacgac gggctggcga taaacgagat aagcctagct ctattttttc cttagataat
     2221 ttgaataaca tgtttccatt gtaccataaa aagttcgaaa tgacttagat aggataaggc
     2281 aaaatctact aacaccaagc ctaaaaaatg ccaccttttt attgatagct taggcaagaa
     2341 caatacttga tagatgtgac tataggtcaa aaaagcaaga caggagcttt taacgccagc
     2401 tactctatat gcttttcagg agaaaatttc tctttttgat tataggcaat gagaaccaag
     2461 gcatcgctta gaaagctagt c
//
LOCUS       DAVBNO010000102         2364 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVBNO010000102 DAVBNO010000000
VERSION     DAVBNO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.102"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..1523
                     /gene="phtA"
                     /locus_tag="VQ846_002030"
     CDS             <1..1523
                     /gene="phtA"
                     /locus_tag="VQ846_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET3076546.1"
                     /translation="FDPAQITSRTARGVAVPHGDHYHFIPYSHMSELEERIARIIPLR
                     YRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSNSSLVSQLVRKVGEGYVFEEK
                     GISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKENVAPRDQEFYDKAYNLLTEA
                     HKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDLLAFLAPITHPERLGKPNSQI
                     EYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYVTPHMGHSHWIGKDSLSDKEK
                     VAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVKGEKRIPLVRLPYMVEHTVEV
                     KNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLFATIKYYVEHPDERPHSNDGW
                     GNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVPQVETEKVEAQLKEAEVLLAK
                     VTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKLLALLKGSNPSSVSKEKIN"
     gene            1681..>2364
                     /locus_tag="VQ846_002031"
     CDS             1681..>2364
                     /locus_tag="VQ846_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET3076547.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAA"
BASE COUNT          831 a          431 c          476 g          626 t
ORIGIN      
        1 ttttcgaccc agcgcaaatc acaagtcgaa cagctagagg tgttgcagtg ccacacggag
       61 atcattacca cttcatccct tactctcaca tgtctgaatt ggaagaacga atcgctcgta
      121 ttattcccct tcgttatcgt tcaaaccatt gggtaccaga ttcaaggcca gaacaaccaa
      181 gtccacaacc gactccggaa cctagtccag gcccgcaacc tgcaccaaat cttaaaatag
      241 actcaaattc ttctttggtt agtcagctgg tacgaaaagt tggggaagga tatgtattcg
      301 aagaaaaggg catctctcgt tatgtctttg cgaaagattt accatctgaa actgttaaaa
      361 atcttgaaag taagttatca aaacaagaga gtgtttcaca cactttaact gctaaaaaag
      421 aaaatgttgc tcctcgtgac caagaatttt atgataaagc atataatctg ttaactgagg
      481 ctcataaagc cttgtttgaa aataagggtc gtaattctga tttccaagcc ttagacaaat
      541 tattagaacg cttgaatgat gaatcgacta ataaagaaaa attggtagat gatttattgg
      601 cattcctagc accaattacc catccagagc gacttggcaa gccaaattct caaattgagt
      661 atactgaaga cgaagttcgt attgctcaat tagctgataa gtatacaacg tcagatggtt
      721 acatttttga tgaacatgat ataatcagtg atgaaggaga tgcatatgta acgcctcata
      781 tgggccatag tcactggatt ggaaaagata gcctttctga taaggaaaaa gttgcagctc
      841 aagcctatac taaagaaaaa ggtatcctac ctccatctcc agacgcagat gttaaagcaa
      901 atccaactgg agatagtgca gcagctattt acaatcgtgt gaaaggggaa aaacgaattc
      961 cactcgttcg acttccatat atggttgagc atacagttga ggttaaaaac ggtaatttga
     1021 ttattcctca taaggatcat taccataata ttaaatttgc ttggtttgat gatcacacat
     1081 acaaagctcc aaatggctat accttggaag atttgtttgc gacgattaag tactacgtag
     1141 aacaccctga cgaacgtcca cattctaatg atggatgggg caatgccagt gagcatgtgt
     1201 taggcaagaa agaccacagt gaagatccaa ataagaactt caaagcggat gaagagccag
     1261 tagaggaaac acctgctgag ccagaagtcc ctcaagtaga gactgaaaaa gtagaagccc
     1321 aactcaaaga agcagaagtt ttgcttgcga aagtaacgga ttctagtctg aaagccaatg
     1381 caacagaaac tctagctggt ttacgaaata atttgactct tcaaattatg gataacaata
     1441 gtatcatggc agaagcagaa aaattacttg cgttgttaaa aggaagtaat ccttcatctg
     1501 taagtaagga aaaaataaac taatgaaaaa tgaaagtctc gataaagagg ctttcatttt
     1561 tattatgtat atatgtaaaa ttcttgacaa gcaatattaa aaagagtaaa ctattaacta
     1621 gttaattaac cggtttatta ctttatagtg aatcaaatat acttaagaaa agaggaaaga
     1681 atgaaaatca ataaaaaata tctagctggg tcagtagcta cacttgtttt aagtgtctgt
     1741 gcttatgaac taggtttgca tcaagctcaa actgtaaaag aaaataatcg tgtttcctat
     1801 atagatggaa aacaagcgac gcaaaaaacg gagaatttga ctcctgatga ggttagcaag
     1861 cgtgaaggaa tcaatgccga acaaatcgtc atcaaaataa cagaccaagg ctatgtcact
     1921 tcacatgggg accactatca ttattacaat ggtaaggttc cttatgacgc tatcatcagt
     1981 gaagaattgc tgatgaagga tccaaactac cagctaaagg acgaggatat tatcagtgaa
     2041 atcaagggtg gttatgtgat taaggtagat ggaaaatact atgtttacct taaggatgca
     2101 gctcatgcgg ataatgtccg tacaaaagaa gaaatcaatc gacaaaaaca agagcatagt
     2161 caacatcgtg aaggtgggac tccaagaaac gatggtgctg ttgccttggc acgttcacag
     2221 ggacgctaca ccacagatga tggttatatc tttaatgctt ctgatatcat tgaagatact
     2281 ggtgatgctt atatcgttcc tcacggcgac cattaccatt acattcctaa gaatgagtta
     2341 tcagctagcg agttagctgc tgca
//
LOCUS       DAVBNO010000103         2359 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVBNO010000103 DAVBNO010000000
VERSION     DAVBNO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.103"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            72..610
                     /locus_tag="VQ846_002032"
                     /pseudo
     CDS             72..610
                     /locus_tag="VQ846_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            677..1087
                     /locus_tag="VQ846_002033"
     CDS             677..1087
                     /locus_tag="VQ846_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET3076548.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            1203..>2359
                     /gene="pavB"
                     /locus_tag="VQ846_002034"
     CDS             1203..>2359
                     /gene="pavB"
                     /locus_tag="VQ846_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET3076549.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKALWSDNGDAKNPALSPLGENVKTKGQY"
BASE COUNT          719 a          464 c          528 g          648 t
ORIGIN      
        1 gcgacgttga cgcggtttga agagattttc gaagagtata agaaaaaatc agtcccctaa
       61 aggagcagat tatgaagtta ttgtctatcg ccatttctag ctataatgca gcagcctatc
      121 ttcattactg tgtggagtcg ctagtgattg gtggtgagca agttgggatt ttgattatca
      181 atgacgggtc tcaggatcag actcagaaat cgctgagtgt ttagctagca agtatcctaa
      241 tatcgttaga gccatctatc aggaaaataa aggccatggc ggtgcggtca atcgtggctt
      301 ggcagaggct tctgggcgct attttaaagt agttgacagt gatgactggg tggatcctcg
      361 tgcctacttg aaaattcttg aaaccttgca ggaacttgag agcaaaggtc aagaggtgga
      421 tgtctttgtg accaattttg tctatgaaaa ggaagggcag tctcgtaaga agagtatgag
      481 ttacgagtca gtcttgcctg ttcggcagat ttttggctgg gaccaggtcg gaaatttctc
      541 caaaggccag tataccatga tgcactcgct gatttatcgg acagatttgt tgcgtgctag
      601 ccagttctaa ctgcctgaac atacttttta tgtcgataat ctctttgtct ttacgcccct
      661 tcagcaggtc aagaccatgt actatctgcc tgtcgatttc tatcgttatt tgattgggcg
      721 tgaggaccag tctgtcaatg agcaagtgat gattaagtgc attgaccagc aactcaaggt
      781 caatcgactc ttggtagacc aacttgattt gtcccaagtg agtcatccca aaatgcgaga
      841 atatctgctg aatcatattg aaatcacgac ggtgatttcc agtaccctgc tcaaccgatc
      901 tggaacagcg gagcatctgg caaaaaaacg ccaattgtgg acctatattc agcagaaaaa
      961 tccagaagtc tttcaggcta ttcgtaagac catgttgagc cgtttgacca aacattctgt
     1021 cttgccagat cgcaaactgt ccaatgtcgt ctatcaaatc accaaatctg tttatggatt
     1081 taattaatat aagtgtttta taagagggat ttaagaaaaa ttttaacttt ttcttagtcc
     1141 tttttaattt caggagatta tactagagtc atcaaataaa gaaagactct aaggagaatc
     1201 ctatgaaatt caatccaaat caaagatata ctcgttggtc tattcgccgt ctcagtgtcg
     1261 gtgttgcctc agttgttgtg gctagtggct tctttgtcct agttggtcag ccaagttctg
     1321 tacgtgccga tgtggtcaat ccgaccccag gtcaagtctt acctgaagag acatcgggaa
     1381 cgaaagaggg tgacttatca gaaaaaccag gagacaccgt tctcactcaa gcgaaacctg
     1441 agggcgttac tggaaatacg aattcacttc cgacacctac agaaagaact gaagtgagcg
     1501 aggaaacaaa ctcttctagt ctggatacac tttttgaaaa agatgaagaa gctcaaaaaa
     1561 atccagagct aacagatgtc ttaaaagaaa ctgtagatac agctgatgtg gatgggacac
     1621 aagcaagtcc agcagaaact actcctgaac aagtaaaagg tggagtgaaa gaaaatacaa
     1681 aagacagcat cgatgttcct gctgcttatc ttgaaaaagc tgaagggaaa ggtcctttca
     1741 ctgccggtgt aaaccaagta attccttatg aactattcgc tggtgatggt atgttaactc
     1801 gtctattact aaaagcttcg gataatgctc cttggtctga caatggtact gctaaaaatc
     1861 ctgctttacc tcctcttgaa ggattaacaa aagggaaata cttctatgaa gtagacttaa
     1921 atggcaatac tgttggtaaa caaggtcaag ctttaattga tcaacttcgc gctaatggta
     1981 ctcaaactta taaagctact gttaaagttt acggaaataa agacggtaaa gctgacttga
     2041 ctaatctagt tgctactaaa aatgtagaca tcaacatcaa tggattagtt gctaaagaaa
     2101 cagttcaaaa agccgttgca gataacgtta aggacagtat cgatgttcca gcagcctacc
     2161 tagaaaaggc caagggtgaa ggtccattca cagcaggtgt caaccatgtg attccatatg
     2221 aactcttcgc aggtgatggt atgttgactc gtctcttgct caaggcatct gacaaggcac
     2281 tatggtcaga taacggtgac gctaaaaacc cagccctatc tccactaggt gaaaacgtga
     2341 agaccaaagg tcaatactt
//
LOCUS       DAVBNO010000104         2142 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVBNO010000104 DAVBNO010000000
VERSION     DAVBNO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.104"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            129..1622
                     /locus_tag="VQ846_002035"
     CDS             129..1622
                     /locus_tag="VQ846_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET3076550.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            1635..>2142
                     /locus_tag="VQ846_002036"
     CDS             1635..>2142
                     /locus_tag="VQ846_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET3076551.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKGLITKRK
                     LQLDELNLL"
BASE COUNT          755 a          322 c          426 g          639 t
ORIGIN      
        1 gtttgaagag attttcgaag agtataaaat attcaggtga cgcatagata tagttaattg
       61 aagctttgtt tgaaatctga taaaataatg atattactaa gttttaaaaa ctaaagaaaa
      121 gggaagatat gattacaggc gaattaaaaa ataaaatcga tcagctgtgg gaaattcttt
      181 ggacagaagg aaacgcaaat cctttaacaa atattgaaca gttgacttat ctcttattta
      241 tgaaagattt ggatagtgtc gagcttggac gtgaaagtga tgctgaattt ctagggattc
      301 cttatgaggg agtttttcca aaagataaac ctgaataccg ttggtcaact tttaaaaata
      361 taggagatgc tcaggaagtt tatcgtttaa tgactcagga gatttttccg tttattaaaa
      421 atctcaaggg ggatacagat gatacagcct tttcacgata tatgcgagaa gctatttttc
      481 aaataaataa acctgctacg cttcaaaagg caatttctat cttagatgtt tttccaacta
      541 ggggattaga tgtagatttt gataatgaca aacaaagtat tactgatatc ggagatatct
      601 atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa tggacagttc cgtacacctc
      661 gtcacatcat cgatatgatg gttgagttga tgcaaccgac tatcaaagat atcatctcag
      721 atcccgctat gggttctgct ggcttcttag tatctgctag ccgttactta aagcgtaaga
      781 aagatgaatg ggaaaccaat acagataata tcaatcattt tcataatcag atgtttcatg
      841 gaaatgatac ggatacgact atgttgagac ttggggcgat gaacatgatg ctacatggag
      901 tagaaaatcc acaaatcagt taccttgact cgctgtctca agataatgaa gaagccgata
      961 aatatacttt ggttttagca aatcctcctt ttaagggctc acttgactac aattcaacct
     1021 ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga attactcttt ctttctcttt
     1081 tcttgcgaac tttaaaacca ggtggacgag cagcagttat cgtacctgat ggtgtccttt
     1141 ttggttcgtc taaagctcat aaaggaattc gtcaggaaat tgtagagaat cataagcttg
     1201 atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta tgctggagtt tcaactgcca
     1261 ttctcatctt tacaaaaact ggtaatggtg gtactgacaa agtctggttt tacgatatga
     1321 aagcggatgg tttaagtttg gatgataagc gacaaccgat tagcgacaat gatattccag
     1381 atattatcga acgctttcat catcttgaaa aagaagcaga acgtcagaga acggatcaat
     1441 ctttctttgt tccagttgct gagataaagg aaaatgatta tgatttgtct atcaataaat
     1501 ataaagagat tgagtatgaa aaagttgagt atgaaccaac agaggtcatc ttaaagaaaa
     1561 tcaatgattt agaaaaagaa attcaagctg gcttggctga attagaaaaa ttactcaagt
     1621 agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt cttatctcta aaaaaaggca
     1681 agaaagccac tgtacttgct gaacaaacaa ctctaagcca acgttatatt caaatagatg
     1741 atttaagaaa taataataat ttaaaattca ctgaaagttt aaatatgact gaagcactcc
     1801 cagatgatat tctgatagca tgggatggag ctaatgcagg aacagttggt tatggattat
     1861 cgggagctgt tggtagtaca attacggtct taaaaaagaa tgagcgatac aaagaaaaaa
     1921 ttatatcaga ttacttggga gtctttttgg aaagtaaatc gcagtattta cgagaacatt
     1981 caacaggtgc aacaattcct catttaaaca agaatatatt acttgattta caattagaat
     2041 tgctaggtat cgaagaacaa gagaacatta tctgtattct taatacgatt aaagggctta
     2101 ttactaaaag aaaattgcag ttagatgaac taaacttgct cg
//
LOCUS       DAVBNO010000105         1912 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVBNO010000105 DAVBNO010000000
VERSION     DAVBNO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.105"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(400..780)
                     /locus_tag="VQ846_002037"
                     /pseudo
     CDS             complement(400..780)
                     /locus_tag="VQ846_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
     gene            complement(1202..>1912)
                     /gene="phtB"
                     /locus_tag="VQ846_002038"
     CDS             complement(1202..>1912)
                     /gene="phtB"
                     /locus_tag="VQ846_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HET3076552.1"
                     /translation="HQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGS
                     LIIPHYDHYHNIKFEWFDEGLYEAPKGYSLEDLLATVKYYVEHPNERPHSDNGFGNAS
                     DHVQRNKNGQADTNQTEKPNEEKPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESP
                     EESPEESEEPQVETEKVKEKLREAEDLLGKIQNPIIKSNAKETLTGLKNNLLFGTQDN
                     NTIMAEAEKLLALLKESK"
BASE COUNT          557 a          388 c          293 g          674 t
ORIGIN      
        1 caattaacta atttaatcac ttaaaaaaat tcgaacactt tctatacaac tgacagctca
       61 aatctttcag aatagaacaa tactaactat cgagcacccc gtattcataa atatatgtgt
      121 aattctagac ctagaattcc tataaactaa atgctttcat actcttccaa gtaattgatt
      181 gccttaaatt ttaatttttg aaggtttcta aagctagaat agccccatca caatcagttt
      241 tgattgattc acaatttaga aacactatag tttcactcct gttaaaataa aaaggaactg
      301 cataaagcaa tccctttctg attttgaaat catttactta acattttata gttgagataa
      361 tcaatagctt acctataaaa agagttatag taaaattcct tatttattga tttcaagctc
      421 cgctaactgt gtttgaataa ctgacagttc tgcaccagcc tgaagaagag cagctgcatt
      481 ataggcacct tctacaattg gaaccctgtt gatgatgata cttttatcac tgaaatcagt
      541 caccattttt aagttcattt tagcaaaacc taggtcaaaa aaggcaagta aagtatctgc
      601 tgtattttcg gaaacaaccc tatctactta atcaaaactc gttccaattc ctccgccctc
      661 ggttcctcct acataagtaa tcggaacatc tttagctact ttactaatca gttcaacaac
      721 accttctaca atgtatttgg aatgtgaaac gataacaaga ccaataccaa tactttccat
      781 caaaccaccc cagtttctaa aatagcagta aagagtaatc ctgatgagaa tgatccagga
      841 tcaatatgtc caagaaacca catgctccta agacaagagc taacagactg gccatcaata
      901 atagtattgt tctttttttc atcattactc cttaactagt gtttaactga tcaattagcc
      961 agtaaatagt ttatctttat ttacactatc tgttaagata tagtaaaatg aaataagaac
     1021 aggacagtca aatcgatttc taacaatgtt ttagaagtag aggtatacta ttctagtttc
     1081 aatctactat attttgcaca ttttcatgaa aaaaatgaga actagaactc acattctgct
     1141 ctcatttttc gttttcccgt tctcctatcc tgtttttagg agttagaaaa tgctgctacc
     1201 tttacttact ctcctttaat aaagccaata gtttttcagc ttctgccata atagtattgt
     1261 tgtcctgggt gccaaatagt aaattatttt ttaatcctgt gagagtctct ttggcattgg
     1321 acttgataat tggattctgg atttttccaa gtaaatcttc agcctctctc agtttttctt
     1381 taaccttttc agtctcgacc tgaggttctt ctgattcctc tggtgattct tctggtgatt
     1441 cttcttctgg ttcctctgtt ggttttggag actctggttt ctcgctttgc ggtttctctt
     1501 ctcgaggggt ttcttcctca ggtttttctg tctgaggttt ctcctcgttt ggtttttccg
     1561 tttgattggt atcagcttga ccatttttgt ttctttgaac atggtcgcta gcgttaccaa
     1621 aaccattatc tgaatgcgga cgttcgtttg gatgttcgac atagtacttg acagtcgcca
     1681 aaagatcctc aagactatac cccttaggtg cctcataaag gccttcgtca aaccactcaa
     1741 atttgatgtt atggtaatgg tcataatgag gtatgattaa actaccgttt ttgacttcta
     1801 cagtatattg aagattgtaa ggcatacgat caagtggcac cttcttagct gctttcacgc
     1861 ggttgtagat agcttctgct ccttttgcct cagtatttcc tgaatcctga tg
//
LOCUS       DAVBNO010000106         1867 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVBNO010000106 DAVBNO010000000
VERSION     DAVBNO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.106"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(<1..917)
                     /locus_tag="VQ846_002039"
     CDS             complement(<1..917)
                     /locus_tag="VQ846_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET3076553.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREVASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLPYPRKGKALKKQASTNFKPAGQ
                     SIAQRPEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLSDVFSEEHIYYAHPYASWERGTNENHNRFIRR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCL"
     gene            complement(1362..1760)
                     /locus_tag="VQ846_002040"
     CDS             complement(1362..1760)
                     /locus_tag="VQ846_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET3076554.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          504 a          379 c          325 g          659 t
ORIGIN      
        1 tccaagcatt ttttaggata gttgttaatc cacttttcga tgaatgcgac ttctttggga
       61 gtcattttct tggttccctt aggtaaccat ctacgaatga acctgttgtg attctcatta
      121 gttccccttt cccaagaggc ataggggtgc gcataataaa tgtgctcctc agaaaataca
      181 tcagacaagc gattgaattc cgttccatta tctgccgtga tggaaagaat cttgtgttgt
      241 tttaagatga gttttagagc ctgattgacc acctcagcac ttttatttgg aatcaatcgg
      301 atgatctgat gtctactctt tcgatccgtc aagacaagca agcagtagtt tttcgctctc
      361 gtaagtagaa ccgtatcaat ctcataatgc ccattctcca agcgaagatt gatagcttca
      421 ggccgctgtg cgatggattg accagcaggt ttaaagttgg tgctggcctg tttcttaagc
      481 gcttttcctt ttctagggta aggcaaatcc tgcttgctta accccaattt tccatgatga
      541 atccaatagt aaatggttga aattcccacg ttaacccctt tagccatcac catcatttca
      601 ggcgaaaatt tttggttatg atagtggaga atcttttcct ttagttcctt ggtcaagctt
      661 gatttcttga ccgagcgctt gcgattgttt tcataagact gttgagcgta gtcggcagaa
      721 taaacctctt tgaagcgccc ttttccaaga cattgtcgga ctgtcccacg cttgatttca
      781 gtgtggatag tttgaggagc ttttccaagt agagaggcaa cttctctatt tgattttcct
      841 tcttttttcc atctttcgat taagcgacgg ctatcgattg tcaaatgttt accttttgta
      901 gtataattgt cttgcatttc tgtgcctttc ttgtgtttgt ggttgaacaa caagtataac
      961 acagaggtgt tttcttatgc ctacaagagc tttcattatt tccattttct tttggatttc
     1021 actctattct gaaaaacttg tgtatattta ctgaagctag caagtcttac ctgtaaattt
     1081 aatgaaagca acacaaaatt cgagagggga atctcggatt aatagataga gagtttttag
     1141 tttaaataaa ttgtttaaaa tatcaacaac atcacttctt ttcttaacct gataagtctt
     1201 gattcctaat tttggggcta cgattatatt gtcctcaata tcgtctagaa agacacaatt
     1261 tctaggttat aactggtatt tatcgatagt tactcatata catcagtcca cctccatact
     1321 tatgtgcgag cctctctttg tattatacct ccatactcac cttacagatt cttttggtaa
     1381 taatatcttt gcctaatgta gagatagtct tgcaaagaaa aaacttcctt gtagccatgt
     1441 ttctgataaa agtccggtgc ctggaactgg taagtattga caaaggcaaa acaacaattt
     1501 cgattcttag cttcactttc tgcctgttgc aatagttttg aaccgattcc ttgccctcgc
     1561 agttcctctt ttacaaacaa atactcgatt tctagccaat ttccaaaagt ctctgctatc
     1621 aaacctgcca ggagattgcc cttttcatct tcgacataaa gattaagtgg ctcactttca
     1681 gcctcttctc tttttgaacg gttataaaca cgaatcagat tccctatttc ttgcgattta
     1741 tgtgattcct tattttccaa tctaatgaaa tgaaataaaa catgcgcaaa tcgattaagg
     1801 aatttaatct aatttctaac aatgtcttag aaatcaaagt gtactatttt aacttcaatg
     1861 cactata
//
LOCUS       DAVBNO010000107         1860 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVBNO010000107 DAVBNO010000000
VERSION     DAVBNO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.107"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(3..968)
                     /locus_tag="VQ846_002041"
     CDS             complement(3..968)
                     /locus_tag="VQ846_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET3076555.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1086..1499)
                     /locus_tag="VQ846_002042"
     CDS             complement(1086..1499)
                     /locus_tag="VQ846_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HET3076556.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(1496..1732)
                     /locus_tag="VQ846_002043"
     CDS             complement(1496..1732)
                     /locus_tag="VQ846_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HET3076557.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
BASE COUNT          541 a          363 c          276 g          680 t
ORIGIN      
        1 ttttaagaga tgaatttttg gtgtgaatgc ttgacattat tttgattgac catggcatag
       61 gccagggttg tgtcaatctt gctatgacct agcagttttt ggacttgttc gataggcata
      121 cctttatcaa ttgccttagt cgctaaagtt cttctgaact tatgtgggtg aaccttttgt
      181 atgccgagtt tgtctcctaa ctttcttaag cgaatctcta caccagcaat tccaagtctc
      241 tgggcttttc caactagcgt tacaaaaaga gcagggtgac tatcttttct gtcgttaaga
      301 taatttctta aatgaatttt cgtccgagcg tcaaaatata ctggtctctc cttctttcct
      361 ttaccaaaga caacacactc tctgttttca aaatcaatat ctgaacgatt caactgtaca
      421 agctccccta cacgcatacc tgtcgatgct agtaggtcta ttattgccaa atctctcaaa
      481 ttttcacagt tatcacgcat aatttccaaa tgttcatcag tataagtttc cttcacattt
      541 tgctcagtct taattttctt tatccgtcga atgggatttt taatgatata ttcctcttgc
      601 tcaagccaag caaaaaaaga agacaatata cgcctaatat tgtctaaatt tgccttacta
      661 caatcctttt cagactggta atttgctaaa taactcctta aatcatctgt tgtgagttgt
      721 gtcacagact ctttaatagc attaaacaag ttttcaatcg tcctctggta ataacgaatt
      781 gtttttgagc tacagccctc tacctgcttg gcggtaataa atagttgcaa caggttatta
      841 ttcgaatact tctgctgctt ttttccgtct actagatgat gttctaaact ctctctcaac
      901 gctagtaatt gttcattatt taaaagaggt aacatttctt gcatgataat tgtaactttt
      961 gattccataa tttctcactt tcttattcat caataaaaag agacaatatc agtttctgca
     1021 ttacagaatt ctaatattgt ctgttcattt attttcttgt gctcattcaa atccagcata
     1081 ctaggttact ttttgacctt ttctctaaca gaatgttcag aaacccattt ggagtagctt
     1141 gtcatttttt catcagttaa agcttctact gcgatggtta cacacatttt tactgcttct
     1201 tctaccgtta cagaaaaacg atagccgttt aattgtaaaa atttgaccaa aacgaagaaa
     1261 gcagttcttt tattagcatt agcaaaaaca tgcttcttta tcaattggac aaatagtatc
     1321 gttgctttat caaaaattgt tggataaaga gacttcccaa agacaaattg ttctggtaag
     1381 ttcacaatca tatttaaggc agaagaacta actgtttgaa ttttctcatt tggagaatac
     1441 cgttgaattg ctaaagcatt tattttttca atttgctttt ctgtcagata gattgtcatt
     1501 tttccaccaa agctttgaaa acatcgtcgt attcgtcaaa tattttatct aaatttaagt
     1561 caaaggcatc atcagataca taagaaacct gctgccccaa ttcttcagga gtaaagataa
     1621 tctcaccact tggtaacaat ttcgcttcgt atttaacacc acttggaata ttaaattcac
     1681 ttggaatcgt aatggtgatt gaatttcctt gttttcttgt ttttactacc atctgatttc
     1741 ctcctttgaa gtattataac aaatcattac cgtaattaca atggcagaat atcagccaaa
     1801 atactcctgc atcagagatt tcttcaaagt ttcaagttct tccagagatt tttggattgc
//
LOCUS       DAVBNO010000108         1646 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVBNO010000108 DAVBNO010000000
VERSION     DAVBNO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.108"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(242..1006)
                     /locus_tag="VQ846_002044"
     CDS             complement(242..1006)
                     /locus_tag="VQ846_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET3076558.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVVNYKDEEICLVQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIERVLD
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEDCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLNQYDSRDWGSEAFNKALELSLASVHHL"
     gene            1228..1524
                     /locus_tag="VQ846_002045"
     CDS             1228..1524
                     /locus_tag="VQ846_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET3076559.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPDAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
BASE COUNT          522 a          404 c          316 g          404 t
ORIGIN      
        1 gaagctgacc tggtttgaag agattttcga agagtatcat ttcatctcac tctaaatctt
       61 accaacataa aacgcatagt atcaaagttt tcatcacctg atatgatacg tttttctgat
      121 tttaaagatt tttcccaacc tcaacatgcc taggacaaga ggaaggtaac ctcgtttaaa
      181 catatttttg aaaagtatga gctaggcatt ttatgataaa acaaatcctt tgccagtaca
      241 actaaaggtg gtgaacactt gctaaactca gttctagcgc cttattaaaa gcttccgagc
      301 cccagtcacg actgtcgtac tggttcaagt ccgctagaga atctgctgtg aacaacaatt
      361 caccccagag aaccccacgc aattgagcta ctgccgcaag agcagaacac tccatctcca
      421 caacagcaca gtcttcttcc ttgcgataag ccaccttttc agccgtttct cggtaaaaac
      481 catctgtgga ccaggtcatg acttcttcat aaggaatccc tctatcatcc aaaactcgct
      541 caatggcagc aatagcctct ggctgcattt ccatataacg agaaggtgcc acatagtggt
      601 aactggctcc ttcatctcgc agagcgcgaa cagggactag aaaggcattt tcctctatat
      661 cagctaggac accacaggtc ccagtagaga taatctgctc cacaccatag ccaatcaacc
      721 aatccataaa ctgggctgct ggagctgaac cgacaggagc ctgaaccaga caaatctcct
      781 cgtccttgta gttcacgaca taaactggat aggtcttggt ggcagaaaca aattcgccaa
      841 cacagttcgc ccctacttcc ctcgcatagc ggtcaatctc ctcacctaaa aaagcataga
      901 cacacttctt tggcaactgc aaatccaacc cctcgtgatt gggcatgata accgcctgag
      961 gattatcatc aaactctaaa ataggaatcg catgtttctg aatcatagcc cacctcctct
     1021 aattttgtac tattgtatca aaatctgaca aagtgtcaag gaggaagctg atgaactgag
     1081 gagaaaaaca agcctacatc gtcagctgcc acagtggttt gcgcagctat atcgcagagc
     1141 ctatcctcaa gcaagcagga tttaccgtcc aaaaccttga cggcgcttat tcactataca
     1201 aaatgactaa cccagaagga gtagaatatg gaaactagta tcagcatggc tgacttttat
     1261 ggaaaatatc aaaatgaaaa tctagaactt atcgatgtcc gtgaagcaca tgaattccaa
     1321 gcaggacatg caccagatgc caaaaatctt ccattaagta ccttagagca aggctacaaa
     1381 gaactcaaac ctgaccatga atactatgtt atttgtcaag gaggagtccg ctctgcatct
     1441 acctgccaat ttctcagctc ccaaggcctc accgttacca acgtagaagg tggtatgaat
     1501 gcttggcccg gtcaagtaga ataatcaaga ggagctgggc aaaaactcaa tttcaggaga
     1561 agatgaagta atactcaatg aaaattaaag tgcaaactag gaagttagcc gcaggctgct
     1621 caaaacactg ttttaaggtt gtggat
//
LOCUS       DAVBNO010000109         1465 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVBNO010000109 DAVBNO010000000
VERSION     DAVBNO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.109"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            84..491
                     /locus_tag="VQ846_002046"
     CDS             84..491
                     /locus_tag="VQ846_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET3076560.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKID"
     gene            656..955
                     /locus_tag="VQ846_002047"
     CDS             656..955
                     /locus_tag="VQ846_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET3076561.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1067..>1465)
                     /locus_tag="VQ846_002048"
     CDS             complement(1067..>1465)
                     /locus_tag="VQ846_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET3076562.1"
                     /translation="SEIIAYNLSCSPNLEQVQTMLEQAFKEKHYENTILHSDQGCQYQ
                     HDSYHRFLESKGIQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKTFKSLNQLEQAI
                     IDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
BASE COUNT          487 a          284 c          258 g          436 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact tctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tccaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattgacta atactcttcg aaaataaaat ttaaaccacg tcagcgtcgc cttaccgtac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcagctag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgttg atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataata aaacgcatag
     1021 tagcaggtgc cgaaatgaac tgcaccccaa aagttagaca attaatttat ccgaaggatt
     1081 tagttctgta ttgcacaggg ctaagtcctt ttagttttac cttaattctc ttgttgttgt
     1141 aataatcaat atagtctata atggcttgtt ccaattgatt aagtgattta aatgttttct
     1201 catagccata aaacatttcg gattttaaaa tgccaaagaa agattccatc ataccgttgt
     1261 cttggctgtt tcccttgcgt gacatggatg cttgaattcc cttactctct aggaaccgat
     1321 gataagaatc gtgttggtat tgacagcctt ggtcactatg gagaatcgta ttctcatagt
     1381 gcttctcttt gaatgcctgt tccaacattg tttgtacttg ttctaaatta ggcgaacaag
     1441 aaagattata ggcgataatt tcgct
//
LOCUS       DAVBNO010000110         1414 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVBNO010000110 DAVBNO010000000
VERSION     DAVBNO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.110"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..464
                     /locus_tag="VQ846_002049"
     CDS             <1..464
                     /locus_tag="VQ846_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3076563.1"
                     /translation="YAMFDKEQKRGYKQSARNLANWHYNDKEDSYTHPDGWYYRFHHT
                     KYQKTQTDFQQKIKVYYADEPESAPQKGLYMNERYQNLKAKECQALLSPQGRQIFAQR
                     KIDVEPVFGQIKACLGYKRCNLRGKRQVRIDMRLVLMANNLLKYNKRTTQN"
     gene            complement(614..808)
                     /locus_tag="VQ846_002050"
                     /pseudo
     CDS             complement(614..808)
                     /locus_tag="VQ846_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(835..>1414)
                     /locus_tag="VQ846_002051"
     CDS             complement(835..>1414)
                     /locus_tag="VQ846_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET3076564.1"
                     /translation="RLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEM
                     LKNPRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGA
                     MPELFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEDLNIG
                     SVRSPLTPVTEEDRPVVEEAAALIRETKERFL"
BASE COUNT          445 a          284 c          287 g          398 t
ORIGIN      
        1 aatatgccat gtttgataag gaacaaaaga gaggctataa acagtcagct agaaacttag
       61 cgaattggca ctataatgac aaggaggata gctatacaca tcctgatggc tggtattatc
      121 gttttcacca taccaaatat cagaaaacac agacagactt tcaacaaaaa atcaaggttt
      181 actacgccga cgaacctgaa tcagcccctc aaaagggact gtatatgaac gaacgctatc
      241 aaaacttgaa agctaaagaa tgtcaggcgc ttttatctcc ccaaggtaga cagattttcg
      301 ctcaacgcaa gattgatgtg gaacctgtct ttgggcagat aaaggcttgt ttgggttaca
      361 agagatgtaa tctgagaggg aagcgtcaag tgagaattga catgagattg gtacttatgg
      421 ccaataacct cctaaaatac aataagagaa cgactcaaaa ttaaaaagct agagttccac
      481 aattggaaat ctttagcttt tttgtgattg agaactattt tatcctagac tcttttgatt
      541 tttaaagaga acttaaatca ttctctaatt aaagtaggaa gaatacacta atttagtaaa
      601 gtaaatatta aatttatttt attttaaaat gatagtaggc tccaagcata tcagcagtgt
      661 tttgatgatg agcaaattct aatcttgtgt tctcagccaa acttggaacc aaggcttctt
      721 tcaaggctgc acgaatcttt ggtttgagga tagcttcttg tcccatgatg ccacccccga
      781 gaatgaccac ctctggattg gcaacgtagt gcgtcataaa tacctcctta tggattagag
      841 gaagcgctcc ttggtttcac gaatcaaggc agcagcttct tctacaactg gacgatcttc
      901 ttcagtcact ggtgtcaatg gtgaacgaac agatccaata ttcaagtctt cattgatctt
      961 caaaacttct ttgatgacac cgtacatatt tccatgagca gaagtgagtt taccaatgat
     1021 tgcgttgata gcatactgca attcacgcgc tgtttctagg tccttatccg caatcaactg
     1081 attgagtttc aagaagagtt ctggcatagc accataagta ccaccgatac cagccctagc
     1141 ccccatgagg cgtcctccta ggaactgctc atcaggacca ttaaagacga tatggtcttc
     1201 tccaccaagg ctgacaaagg tttggatatc ttgaactggc atagaagagt tcttcacacc
     1261 gataacacga ggatttttca acatttctgt gtaaaggctt ggagtcaaag caacccctgc
     1321 caattgagga atgttgtaaa taacgtagtc tgtgtttgga gctgcagaac tgatatcgtt
     1381 ccagtatttg gcaactgagt attctggcaa gcgg
//
LOCUS       DAVBNO010000111         1409 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVBNO010000111 DAVBNO010000000
VERSION     DAVBNO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.111"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            283..1293
                     /gene="ccpA"
                     /locus_tag="VQ846_002052"
     CDS             283..1293
                     /gene="ccpA"
                     /locus_tag="VQ846_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET3076565.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
BASE COUNT          440 a          232 c          318 g          419 t
ORIGIN      
        1 tcctgttctt atttcatttt actataactt taggagggct tagtttcctc ctttttgttt
       61 agtctaaggt ggtagcaagg agctaaacct aggttttcta tgaaattttt tcaatcaatc
      121 ttcaaaaaac acttgaaagt gtttacaatt agtgatagaa tggaataagt agaaatatga
      181 aaacgaaatt ttcacatctt cacttttgta agtagatata gagcttcaat cagcacctct
      241 acttgtaaaa gaatatgtga gatagaaagg acaggaaaag gaatgaatgc agatgataca
      301 gtaaccattt atgatgtcgc tcgtgaagca ggtgtttcca tggcgacggt cagccgtgtg
      361 gtcaatggca ataaaaatgt aaaagagaat acccgtaaaa aagtgctaga ggtaattgat
      421 cgtttggatt atcgtccaaa tgcagttgcg cgtggtcttg caagtaaaaa gacaaccact
      481 gtcggtgtcg tgattccaaa tattaccaat ggttattttt cgagtttggc taaggggatt
      541 gatgatattg cagaaatgta caagtacaat attgtcctag ctaatagcga tgaagataac
      601 gagaaagaag tttctgttgt caataccctc ttttcaaagc aggtagatgg cattatctat
      661 atgggttatc acttgacaga taaaattcgc tcagaatttt cgcgctcacg tactccgatt
      721 gttctcgcag gaactgtcga tgttgagcac cagttgccaa gtgtcaatat tgactataag
      781 caagcaacaa ttgatgcagt gagttacctt gctaaagaaa atgagcgtat tgctttcgtt
      841 agcggtccgc tagtggatga catcaatggt aaggttcgtt tagttggcta caaggaaacc
      901 ttgaaaaaag caggaatcac ttatagtgag ggtttggtat ttgaatctaa atatagctat
      961 gatgatggtt acgccttagc agagcgtttg atttcatcaa atgcaactgc agcagttgtg
     1021 acaggtgatg agttggcagc aggagtcttg aacggtttgg ctgataaagg tgtttctgtg
     1081 ccagaagatt ttgaaattat tactagtgat gattcacaaa tctcacgctt tacccgtcca
     1141 aacttgacaa cgattgccca acctctttat gaccttggtg ctattagtat gcgtatgttg
     1201 accaagatta tgcataagga agagttggaa gaacgtgaag ttctcttacc tcatggtttg
     1261 acagaacgta gctcaacacg aaaacgtaaa tagaaaaaat cagggaatcg agaagatttc
     1321 ctgattttgt gctcttaaac aagagatgtt gacttaatga ataccataga gcggactagg
     1381 cggctggtta aaggctgcgc aacacactg
//
LOCUS       DAVBNO010000112         1267 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVBNO010000112 DAVBNO010000000
VERSION     DAVBNO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.112"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            250..590
                     /gene="tnpB"
                     /locus_tag="VQ846_002053"
                     /pseudo
     CDS             250..590
                     /gene="tnpB"
                     /locus_tag="VQ846_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(623..1267)
                     /locus_tag="VQ846_002054"
                     /pseudo
     CDS             complement(623..1267)
                     /locus_tag="VQ846_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          382 a          251 c          228 g          406 t
ORIGIN      
        1 aatttcataa taaaaacacc ccaaaagtta gattttttct gtctaacttt tggggggcag
       61 ttcattctac acccactatt atgcgttttt atcattttga aatctactgc taatcaatga
      121 agaagcaaag atcaaactag gcagctagtt acaagttacg taagcgcgtc ataacaaggt
      181 atctatccta attccccaac ttccagtata ctagataaaa aataattatc ggaggaaagg
      241 tattgtacta tgataattca actaagtgat ttaggtcaag ttcaccttgt ttgtagcaag
      301 atagatatga ggcagggaat agactcatta gcctatgtag ttaaaaccca ctttgaattg
      361 gatcctttct ccggtcaagt ctttctcttt tgtggtggac gtaaagaccg ctttaaagcc
      421 ctttactggg atggtcaagg attttggcta ctatataaac gctttgagaa cggcagactg
      481 acttggccca gtacagaaaa ggatgtcaaa gctctcacac ctgaacaagt agactggctt
      541 atgaagggct tttctatcac tccaaaaata aatccatcag aaagtcgtga tttctattga
      601 aatgaggact ttctttttat actcatctgc tttcaaaaag cattctagtc catctccgat
      661 taacgatggg ctttatcacc tccttctcca gtccttgtat gatatcttaa agttgattca
      721 tgacatcttc caaagtttga aaggctttat ttttaaatcc acgtttacga atctctttcc
      781 acacttgttc aatggggttc atctctggtg tgtatggagg aataaatgca aagccaatat
      841 tagtcggaat ctttaaggta cttgatttat gccatatagc attgtccata acgaataaaa
      901 gataatcatc tggataagct tgtgaaagct cttctaaaaa gacgttcatc cactcagtat
      961 tacatccacc agctattaag aaaaatgatt cgcctgtatg ggtccataac aatagcgaaa
     1021 ttctcgtata tagtgactat ggacatgtgg acctactcct attggagacc aacaagatcc
     1081 cagtttactg attctaccga agccagcctc atcttggtac atcaagcgaa ccttatgaaa
     1141 acgtctactg gttttaaagc gctttctttt cttcttgaat tgaaatttta tttttagacg
     1201 cgacaatggt ttgagcgtct gctttcttag gatgttctgg acgtggcata atatttcgcc
     1261 aaccatg
//
LOCUS       DAVBNO010000113         1180 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVBNO010000113 DAVBNO010000000
VERSION     DAVBNO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.113"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(<1..96)
                     /gene="rrf"
                     /locus_tag="VQ846_002055"
     rRNA            complement(<1..96)
                     /gene="rrf"
                     /locus_tag="VQ846_002055"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(173..>1180)
                     /locus_tag="VQ846_002056"
     rRNA            complement(173..>1180)
                     /locus_tag="VQ846_002056"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          271 a          344 c          252 g          313 t
ORIGIN      
        1 cacagggggc aacccccaac tacttccggc gttctagggc ttaacttctg tgttcggtat
       61 gggtacaggt gtatctccta ggctatcgtc acttaactct gagtaatacc tactcaaaat
      121 tgaatatcta ttcaattcaa gaaaaccatt cgctttcata ttctcagtta ctttggataa
      181 gtcctcgagc tattagtatt agtccgctac atgtgtcgcc acacttccac ttctaaccta
      241 tctacctgat catctctcag ggctcttact gatatataat catgggaaat ctcatcttga
      301 ggtgggtttc acacttagat gctttcagcg tttatccctt ccctacatag ctacccagcg
      361 atgcctttgg caagacaact ggtacaccag cggtaagtcc actctggtcc tctcgtacta
      421 ggagcagatc ctctcaaatt tcctacgccc gcgacggata gggaccgaac tgtctcacga
      481 cgttctgaac ccagctcgcg tgccgcttta atgggcgaac agcccaaccc ttgggaccga
      541 ctacagcccc aggatgcgac gagccgacat cgaggtgcca aacctccccg tcgatgtgaa
      601 ctcttggggg agataagcct gttatcccca gggtagcttt tatccgttga gcgatggccc
      661 ttccatacgg aaccaccgga tcactaagcc cgactttcgt ccctgctcga gttgtagctc
      721 tcgcagtcaa gctcccttat acctttacac tctgcgaatg atttccaacc attctgaggg
      781 aacctttggg cgcctccgtt accttttagg aggcgaccgc cccagtcaaa ctgcccgtca
      841 gacactgtct ccgataggga tcacctatct gggttagagt ggccataaca caagggtagt
      901 atcccaacag cgtctccttc gaaactggcg tcccgatctc ttagactcct acctatcctg
      961 tacatgtggt acagacactc aatatcaaac tgcagtaaag ctccatgggg tctttccgtc
     1021 ctgtcgcggg taacctgcat cttcacaggt actaaaattt caccgagtct ctcgttgaga
     1081 cagtgcccaa atcattacgc ctttcgtgcg ggtcggaact tacccgacaa ggaatttcgc
     1141 taccttagga ccgttatagt tacggccgcc gtttactggg
//
LOCUS       DAVBNO010000114         1087 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVBNO010000114 DAVBNO010000000
VERSION     DAVBNO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.114"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(796..1056)
                     /locus_tag="VQ846_002057"
     CDS             complement(796..1056)
                     /locus_tag="VQ846_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076566.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELELVKSTILQNDDISLDQNEIFEILKNFFDG"
BASE COUNT          362 a          203 c          174 g          348 t
ORIGIN      
        1 tggtattatc aatcatgaac taggattcta gttttgcagg aagtctatta tatatatgca
       61 cagacatatg aaatcttttt gatacagcct tctttttaca tagagagaca gcagttatga
      121 tatatttatc cattcacaac atcttctgta ccaatactac aataattggt acattttgat
      181 tacacaatcg tttcaaaaaa tatgatggaa gactgagtat tttatagtaa ttcccttcct
      241 atcaactaga tcaaccagtt gagtgttgaa tgttgaaaca ccctagcctc agcaaaggac
      301 tatgatcagc atttcctagc caataggacg ctccaaatca cctattctaa gtccatcact
      361 agcttggtca gtaagataca gactgcaagg caagactgac gtggttgatg agattttgga
      421 agagtattac aatcatagat tcaccagtag acttgaccgc agatttaact atagtagatt
      481 gaaactagaa tagtacactt ctgcttctaa aacattgtta gaaatcgatt tgactgtcct
      541 gatcaatttg tcctgttctc gtttcatttt actatactaa aaaaccacct gaaacaaatc
      601 aagtgactag aggataaaaa caaagactgg gcaaaaagtt ttgaaaagca aaaaacgcat
      661 aatatcaggt cttgaaaaac cttgatacta tacgtttgat tgtgggaaga ttgacttcat
      721 tttctcctga aattgaagtt ttttctagtt tccaggtata gacgaattca atatagccat
      781 ctcgctacaa ttattttaac catcgaaaaa atttttcaga atttcaaaaa tttcattttg
      841 atctaatgaa atgtcatcat tttgaagaat agttgacttc acaagctcta attctatcac
      901 aaagcggtgc atgtctgctt ttaccgcact acttgatgtt atagccgata acttttcatc
      961 tcctagcaag gaagctgtca aacgggtaag gtcattcaaa tgctttttaa tggacttact
     1021 taaaccttga gcacctgtag cagagcgttc cctcatatcc agccaagctt tagccttaaa
     1081 aataatt
//
LOCUS       DAVBNO010000115         1029 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVBNO010000115 DAVBNO010000000
VERSION     DAVBNO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.115"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(225..515)
                     /locus_tag="VQ846_002058"
     CDS             complement(225..515)
                     /locus_tag="VQ846_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076567.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQISERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(503..755)
                     /locus_tag="VQ846_002059"
                     /pseudo
     CDS             complement(503..755)
                     /locus_tag="VQ846_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
BASE COUNT          303 a          193 c          143 g          390 t
ORIGIN      
        1 gtggatgttt ctggtctaaa gtcatctgaa tctttgagat gagctttata aatcgctttt
       61 ttcagttttt gcactggtgt ttcgataaac tcaaactttt tagccgtggt attgcctgat
      121 tttatagtat attgaaacta gaatagtaca cctctacttc taaaacattt ttagaaatcg
      181 atttgactgt cctgatcgat ttgtcctgtt cttatttcat tttactatat ttgagccact
      241 tcgtctttaa cggctttatt cataagctct tgtaattttt ctttactatc aattacttct
      301 gattttccgt tgtaatttat tgtaataggt tttaacttac ctaatttctc gacacgctca
      361 gaaatttgat cttttttgaa ggctgcatat gtttttccta agattttttc gaaaatatat
      421 ttatcagata gcggtttgcc ttcttcttca gcttggtttt tgtattgatt tgaaacataa
      481 ggaacaaatc cttcatagta acctaatgct cccataagtt caaaagcttg ttttctaatt
      541 caaaccattg caactcagat ttcagctttt cagataaatc ctgctcatcc aaataatgac
      601 ttgaaattag tgctgaactc gtttctgtat cctgtacagg ctgagcaccc ataccagcaa
      661 aaaaataaac tcgttcctag caagaccgaa caagctccta ttgcatattt cctcaaagaa
      721 aaacgctgct ttctctcaaa ttgaaattct ttcatcccat ctcccatcat tcattattac
      781 tgtatatttt gtatatcaga aatagtttgt attcacaaat ctttctagtt attcccttat
      841 cattcctaat taagggagat aacatacaat aatttttagt taaatgtata tcgatgtttt
      901 ttgtttttct taataaacgc aatacaaaaa gagcctgttg ccaagctctt tatactcaat
      961 gaaaatcaaa gagcaaacta ggaaactagc cacaggttgc tcaaaacacc gttttgaggt
     1021 agtagataa
//
LOCUS       DAVBNO010000116         1027 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVBNO010000116 DAVBNO010000000
VERSION     DAVBNO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.116"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(1..645)
                     /locus_tag="VQ846_002060"
                     /pseudo
     CDS             complement(1..645)
                     /locus_tag="VQ846_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            640..894
                     /locus_tag="VQ846_002061"
     CDS             640..894
                     /locus_tag="VQ846_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET3076568.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIGAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGEHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
BASE COUNT          302 a          183 c          201 g          341 t
ORIGIN      
        1 aggctttatc ttcagctgtt agcggcttga gtttgctgga tttacgtgga gtttgtgctt
       61 gaggatatat cttcatgagc ccttgataac cactgtcggc caagatttta ccagcttgtc
      121 cgatatttct acaactcatt ttgaacaact tcatatcatg actatagttc acagcgatat
      181 ccaaagaaac aattctccct tgacttgtga caatcgcttg agccttcata gcgtgaaatt
      241 tctttttacc agaatgattc gctaattgtt ttcttagggc gattgatttg tacttccgtc
      301 gcatcaatca ttaccgtgtc ctcagaactg agaggagttc ttgaaatcgt aacaccacct
      361 ttgaacaaga gttacttcaa cccattggct ccgacggagt aagttgcttt cgtggatacc
      421 aaaatcagcc gcaatttatt cataagtgcg atattctcgc acatattgaa gagtggccat
      481 aagaaggtct tctaggctta atttaggttt tcgtccacct tttgcgtgtt taagttgata
      541 agctgttttt aatacagcta atatctcttc aaaagtcgtg cgctgaacac aaacaagacg
      601 cttaaatcgt gcatcagtta gttgtttact tgcttcataa ttcatagaac tactatacca
      661 tattttgttt cgcaggaagt ctattggaaa gtaagaaata ttggagctga ggcgattaga
      721 agaaattgtg agcgtggtgc tattttttca ggtaaaataa aacatcacga agattctcag
      781 tttaaaggag agcactatgt tgaatgttat gctgttttag ataatacggt tatagcaaga
      841 gatagaataa cagtccctat cgatccgtta tgtggaaaag attttataga gtagcatata
      901 attgattctt aactggaata ctctctatct ctttacatca agaaaatgac taaacaggga
      961 agtttgcctt cttccctttt tttgttatac tagtagaaga aaaaaataga aagatttgtg
     1021 ggagtga
//
LOCUS       DAVBNO010000117         1003 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVBNO010000117 DAVBNO010000000
VERSION     DAVBNO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.117"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            77..945
                     /locus_tag="VQ846_002062"
                     /pseudo
     CDS             77..945
                     /locus_tag="VQ846_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          319 a          208 c          164 g          312 t
ORIGIN      
        1 cctattttgt tgtagaaaaa aagtcccata tgacctataa tgaaaagcga ccaaacaact
       61 cattagaaag attcatatgg aacaattaca ttttatcaca aaactgctcg atattaaaga
      121 cccaaacatc aagattctag atatcatcaa tatggatacc cacaaagaaa ttatcgctaa
      181 gctggattat gaggctccat cttgccctga ttgtggaagt ctaatgaaga aatatgactt
      241 tcaaaaaccg tctaagatcc cttatcttga aacgactggt atgcctacta gaattctcct
      301 tagaaagcgt cgattcaagt gctatcactg ttcaaaaatg atggtcgctg aaacttctat
      361 cgtcaagaag aatcaccaaa tccctcgtat catcaaccaa aagattgctc aaaagttaat
      421 tgaaaagatt tctatgactg atattgccca tcagctttcc atctcaactt caactgttat
      481 tcgtaagctc aatgattttc actttaaaca tgatttttct tgtcttcctg agattatgtc
      541 ttgggatgag tatgctttta caaagggaaa gatgagcttc attgcgcaag attttaacaa
      601 tctcaatatc atcactgttc ttgagggtag aacacaagct atcattcgaa atcactttct
      661 tcgctacgat agagttgtcc gatgtcgcgt caaaatcatt actatggata tgtttagccc
      721 ttactatgac ttggctaaac agtttcgctt tcaaatttct aggctcaggc tgaaacagtc
      781 tcccagactg tttcactccc gaatgctaaa atcgtgttgg atcgctttca cattgtacaa
      841 catcttagcc gtgctatgag tcgtgtgcgt gttcaaatca taaatcagtt tcatcgaaaa
      901 tcccatgaat acaaggctat caagcgctac tggaaactca ttcaatagga tagtcgtaaa
      961 ctgagtgata aacattttta tcgtcctact tttcgtatgc att
//
LOCUS       DAVBNO010000118          795 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVBNO010000118 DAVBNO010000000
VERSION     DAVBNO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.118"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            388..>795
                     /locus_tag="VQ846_002063"
     CDS             388..>795
                     /locus_tag="VQ846_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET3076569.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEHLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKNLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGH"
BASE COUNT          279 a          119 c          146 g          251 t
ORIGIN      
        1 tggactaaag ttgtacaaga aaagtgcaaa taagaaatct ccagattagg aactatatat
       61 gagttcacta atctggagat ttttcaatag acttcgttat tgggcggtta ggatatgatc
      121 actgacttcg tcagtcttat ctacaacctc aaaacagtgt tttgagcaac ctgcggctag
      181 cttcctagtt tactctttga ttttcattga gtattagaac agaaaaaatg cttggagtat
      241 ttgtttgtgt gtttattttt atataacaaa ctataaacaa aacaaaaata taaaaaaaga
      301 gacaaaaaag aacagaaagt aattgacaac ggtttcatat agtgttatac ttatggcata
      361 aagttaatgg aaagaaggga aaaacttatg gtattagatt atttctttga caaaaacctt
      421 gtgttttgct tagaagcgga taatcaagaa catctctttg atcaggttgc aactttattg
      481 gaagaacgag aaattgtaac tccaacttat cgtgaagcct tgatcacgcg tgaaaagtca
      541 tttccaactg gtttagatat ggaatttcta ggaaagaact tgccaaatgt agcgattcct
      601 cacacagata ttgttcataa tctagctgag aaagtggtgg ttgttcgatt agaaaaacca
      661 gtaacttttc acaatatgat agctcctgat aaggaagtag aagtatccct actcttcttt
      721 attattaaca attcaagttc aagtcaaaca aatattctgg ctcagttgat ggactttttc
      781 acaggaaatg gacat
//
LOCUS       DAVBNO010000119          765 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVBNO010000119 DAVBNO010000000
VERSION     DAVBNO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.119"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(74..147)
                     /locus_tag="VQ846_002064"
     tRNA            complement(74..147)
                     /locus_tag="VQ846_002064"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(111..113),aa:Arg,seq:acg)
     gene            complement(157..242)
                     /locus_tag="VQ846_002065"
     tRNA            complement(157..242)
                     /locus_tag="VQ846_002065"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(206..208),aa:Leu,seq:taa)
     gene            complement(250..321)
                     /locus_tag="VQ846_002066"
     tRNA            complement(250..321)
                     /locus_tag="VQ846_002066"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:gcc)
     gene            complement(344..416)
                     /locus_tag="VQ846_002067"
     tRNA            complement(344..416)
                     /locus_tag="VQ846_002067"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Thr,seq:tgt)
     gene            complement(435..516)
                     /locus_tag="VQ846_002068"
     tRNA            complement(435..516)
                     /locus_tag="VQ846_002068"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(480..482),aa:Leu,seq:tag)
     gene            complement(522..594)
                     /locus_tag="VQ846_002069"
     tRNA            complement(522..594)
                     /locus_tag="VQ846_002069"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(559..561),aa:Lys,seq:ttt)
     gene            complement(619..691)
                     /locus_tag="VQ846_002070"
     tRNA            complement(619..691)
                     /locus_tag="VQ846_002070"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Asp,seq:gtc)
     gene            complement(694..765)
                     /locus_tag="VQ846_002071"
     tRNA            complement(694..765)
                     /locus_tag="VQ846_002071"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(731..733),aa:Val,seq:tac)
BASE COUNT          195 a          225 c          168 g          177 t
ORIGIN      
        1 cgaacctgcg acaccttggt cccaaaccaa gtactctacc aagctgagct acttcccgag
       61 ttaaatagaa aaatgcaccc tagaggagtc gaacctctaa ccgcctgatt cgtagtcagg
      121 tactctatcc agttgagcta agggtgctcc atattatgcc gaggaccgga atcgaaccgg
      181 tacgatcgtt accaatcgca ggattttaag tcctgtgcgt ctgccagttc cgccaccccg
      241 gcctctctaa gcgaacgacg ggattcgaac ccgcgacccc caccttggca aggtggtgtt
      301 ctaccactga actacgttcg cactgttttc ttctatctaa aaatgccggc tacatgactt
      361 gaacacgcga ccctctgatt acaaatcaga tgctctacca actgagctaa gccggctcat
      421 ttgttatatc ttaatgcggg ttaagggact tgaaccccca cgccgttaag cgccagatcc
      481 taaatctggt gcgtctgcca attccgccaa acccgcatat atgacccgta ctgggctcga
      541 accagtgacc cattgattaa aagtcaattg ctctaccaac tgagctaacg agtctaaaat
      601 aacttgcgtt atcttaaacg gtcccgacgg gaatcgaacc cgcgatcttc gccgtgacag
      661 ggcgacgtga taaccgctac actacgggac ctttgggagt taacgggatc gaaccgctga
      721 ccctctgctt gtaaggcaga tgctctccca gctgagctaa actcc
//
LOCUS       DAVBNO010000120          759 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVBNO010000120 DAVBNO010000000
VERSION     DAVBNO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.120"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..>759
                     /locus_tag="VQ846_002072"
     CDS             <1..>759
                     /locus_tag="VQ846_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET3076570.1"
                     /translation="DGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTS
                     SIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLI
                     ASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLS
                     EQQRIVEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDE
                     SVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQG"
BASE COUNT          297 a          110 c          121 g          231 t
ORIGIN      
        1 tgatggaagt actcaagaaa ttgatgttcc ttatgatatt cctgatactt gggagtgggt
       61 gaggataaaa tcaatttatt ggaattttgg gcaaaataag ccagagaaat cctttaggta
      121 tatagatacg tctagtattg atagaaaaaa gaacataatc aactacaaaa atctacaata
      181 tctttcacct gaacaagcgc cttcccgtgc cagaaaatta gtttcgcaga atagtgtctt
      241 attttcaaca gttagaccat atctaaaaaa tattgctgta gttagagaac ttaaagagta
      301 tttgatagct agtacagcat ttattgtttt ggatacttta cttaacgaaa catatttgaa
      361 atattattta ttatcagata attttatcaa tcgtgttaat aataaatcta ctggaacaag
      421 ttatcctgca atcaatgatt ataattttaa tctattatta attgctctcc ccccactatc
      481 cgaacaacaa cgaatagtag aagcaatcga atcagcttta gaaaaagtag atgaatatgc
      541 tgaaagttat aatagactag aacagctaga taaagaattt ccagataaac taaaaaaatc
      601 tattcttcaa tatgctatgc aaggaaaatt agttgaacaa gacccaaatg atgaatcagt
      661 cgaagtttta cttgaaaaaa tacgagcaga aaaacaaaaa ctctttgaag aaggcaagat
      721 taaaaagaaa gatttggaca tttctattgt ttcccaagg
//
LOCUS       DAVBNO010000121          700 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVBNO010000121 DAVBNO010000000
VERSION     DAVBNO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.121"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            3..305
                     /locus_tag="VQ846_002073"
     CDS             3..305
                     /locus_tag="VQ846_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET3076571.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVPVFDNIRLPAKQNMV"
     gene            313..>700
                     /locus_tag="VQ846_002074"
     CDS             313..>700
                     /locus_tag="VQ846_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HET3076572.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEILAVLKTAYQLKHAKGG
                     RKPKLSLEDFLMATLQYVREYRTYEEIAADFGIHESNLIRRSRWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKTISE"
BASE COUNT          230 a          131 c          144 g          195 t
ORIGIN      
        1 aaatgattaa aattcttgct gcctgcggtg caggtgttaa ctcaagtcac caaattaaaa
       61 gtgctctaga agaagaactt tcaaaccgtg gttatgatgt tcactgtgat gcagttatgg
      121 tgaaagatgt aaacgaagac cttatgaaag gttacgatat ctttacacca atcgctgcaa
      181 cagaccttgg ttttgaacca ggtatcccag ttatcgaagc tgggccaatc ttattctgta
      241 tcccagcaat gagcgttcca gtatttgaca atattagact tcctgcgaaa caaaatatgg
      301 tatagtagtt ccatgaatta tgaagcaagt aaacaactaa ctgatgcacg atttaagcgt
      361 cttgttggtg ttcaacgcac gacttttgaa gagatattag ctgtattaaa aacagcttat
      421 caacttaaac acgcaaaagg tggacgaaaa cctaaattaa gcctagaaga ctttcttatg
      481 gccactcttc aatatgtgcg agaatatcga acttatgaag aaattgcggc tgattttggt
      541 atccacgaaa gcaacttaat ccgtcggagc cgatgggttg aagtaactct tgttcaaagt
      601 ggtgttacga tttcaagaac tcctctcagt tctgaggaca cggtaatgat tgatgcgacg
      661 gaagtacaaa tcaatcgccc taaaaaaaca attagcgaat
//
LOCUS       DAVBNO010000122          630 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVBNO010000122 DAVBNO010000000
VERSION     DAVBNO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.122"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            47..408
                     /locus_tag="VQ846_002075"
                     /pseudo
     CDS             47..408
                     /locus_tag="VQ846_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
BASE COUNT          207 a           76 c          119 g          228 t
ORIGIN      
        1 ggctagtttc ctagtttgct ctttggtttt cattgagtat aacacattgt tagaagttgg
       61 tttaaatttc ctaatcagtt tgttcacatt taccttcgat atattatatc ccatagttaa
      121 ggttggtcat acagatgatt atagtcatgg agccgtaaaa cttagtgttt ctttagttga
      181 caaagatgcc atgaaaaaat atctgtaact gtaataggat attttgaaat aaatatagat
      241 gaaaatatca ccgatattct atacgtaaat ggtactgcta ttctttgtcc ttatttacgt
      301 tctattgttt caatagtttc ggcaattgat agcagtgaag caatgttgct acctaccatt
      361 aatgttttag agttactaga taaatctcaa ccttttgaag aagaataatt tattagctca
      421 ctaaattgag ggtaaggaaa agtaaaagca gtaagaaaaa tgtcttgcat tatacagcaa
      481 ccttttggga atgagtagat ggattgaata aaatttgatt aagagtggat gatttatctg
      541 tagattatta ttggacagtt agtcttgaag tagtctaaga attaggttat aatcagtaga
      601 agccttgcta ataatgagga ggttagttta
//
LOCUS       DAVBNO010000123          601 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVBNO010000123 DAVBNO010000000
VERSION     DAVBNO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.123"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..>601
                     /locus_tag="VQ846_002076"
     CDS             <1..>601
                     /locus_tag="VQ846_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET3076573.1"
                     /translation="FDPAQITSRTANGVAVPHGDHYHFIPYSQLSPLEEKLARIIPLR
                     YRSNHWVPDSRPEQPSPQPTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYV
                     FEENGVPRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDL
                     LARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLV"
BASE COUNT          192 a          135 c          122 g          152 t
ORIGIN      
        1 ttttcgaccc agcgcaaatc acaagtcgaa ccgccaatgg tgttgctgta ccgcacggag
       61 accattatca ctttattcct tattcacaac tgtcaccttt ggaagaaaaa ttggctcgta
      121 ttattcccct tcgttatcgt tcaaaccatt gggtaccaga ttcaagacca gaacaaccaa
      181 gtccacaacc gactccggaa cctagtccaa gtccgcaacc tgcaccaaat cctcaaccag
      241 ctccaagcaa tccaattgat gagaaattgg tcaaagaagc tgttcgaaaa gtaggcgatg
      301 gttatgtctt tgaggagaat ggagttcctc gttatatccc agccaaggat ctttcagcag
      361 aaacagcagc aggcattgat agcaaactgg ccaagcagga aagtttatct cataagctag
      421 gagctaagaa aactgacctc ccatctagtg atagagaatt ttacaataag gcttatgact
      481 tactagcaag aattcatcaa gatttacttg ataataaagg tcgacaagtt gattttgagg
      541 ctttggataa cctgttggaa cgactcaagg atgtctcaag tgataaagtc aagttagtgg
      601 a
//
LOCUS       DAVBNO010000124          599 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVBNO010000124 DAVBNO010000000
VERSION     DAVBNO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.124"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(21..500)
                     /locus_tag="VQ846_002077"
     CDS             complement(21..500)
                     /locus_tag="VQ846_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET3076574.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          160 a          139 c           78 g          222 t
ORIGIN      
        1 attttttttt gcatgactta ctagtgggta cggatagtaa actccttaaa cacaatgcgt
       61 aagtctctta atactctttg acgccctcga aatcgttcat tgcttaagac gcgttctagt
      121 tcttcttgtt tctctttact ttgtttgttt ctataatctc ttagtgtttc atgaaagaga
      181 taataatcat ctagccagag acccccctca cttatacgat gactgatctc tcccacttct
      241 tcatagggtt ctttatcgta tctacgcttc tgactttcct gcttacggat atagtctaaa
      301 attcgatttc gaaacttggt tttgaaatat ttccttaaac gtggaatatc gtctaccagt
      361 ccttcttctc tactaatcaa ttcatgtaag cagagcatgc cttcttggtc ccaatccgac
      421 aattcccata aatgaaggta atattcattt ctacacttat aaacagtccc ttggacttct
      481 tcatacaatt ctttaatcat aattttcccc tttctagata cagtctaaca gattagaaaa
      541 cacatttgtt ccattttctc cattctgttc cctttagctc ctaaactgca caaaaaaga
//
LOCUS       DAVBNO010000125          536 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVBNO010000125 DAVBNO010000000
VERSION     DAVBNO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.125"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(<1..534)
                     /locus_tag="VQ846_002078"
     CDS             complement(<1..534)
                     /locus_tag="VQ846_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET3076575.1"
                     /translation="MEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAAEQGGAVGIR
                     ANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIEVIALDCTKR
                     ERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTLSGYTSYSPK
                     VDGPDFELIKKLCDAGVD"
BASE COUNT          141 a          133 c          103 g          159 t
ORIGIN      
        1 atctacaccg gcatcacaga gtttcttaat caattcaaaa tctggaccgt ctacttttgg
       61 actgtaggat gtgtagcctg ataaggttgt tccgacaaag tcaattcctg cttctacagc
      121 tgctagccct tcttcgaaga tactagtatc agccatcaaa agctgattag gatatttctc
      181 cttaacctga cgaatgaact cttgaatttc caaaccatcg tagcgttcac gcttggtaca
      241 atccagagca atcacctcga tgtccagttc tgccaattca tcaacttctt tcatagtagc
      301 cgtgatgaag ggttcctgag gtggataatc acgtttgata atcccaatga ttggaagttt
      361 agtgacttcc ttaatttcct tgatatcgcg aacactgttt gctcggatac cgactgctcc
      421 accttgctca gccgctttga ccagcaaggg aatcacccct cccgcttctg tataaagcgg
      481 ttcatgagga agagcctgac aagaaacgat gattccatct ttgatttgct caatca
//
LOCUS       DAVBNO010000126          535 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVBNO010000126 DAVBNO010000000
VERSION     DAVBNO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.126"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(<1..110)
                     /locus_tag="VQ846_002079"
     CDS             complement(<1..110)
                     /locus_tag="VQ846_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3076576.1"
                     /translation="MVNCEPLEAYRQLAEAELVGCWAHVRRKFFEATPKQA"
     gene            complement(183..410)
                     /locus_tag="VQ846_002080"
     CDS             complement(183..410)
                     /locus_tag="VQ846_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3076577.1"
                     /translation="MRERLLTQPLLHADETSYRVLESHSHLTYYWTFLSGKVENQAIT
                     LYHHDQSRSGSVVQEFLGDYSGYVHCDMLRQ"
BASE COUNT          155 a          125 c          108 g          147 t
ORIGIN      
        1 gcttgcttgg gggtcgcttc aaaaaatttc cttctcacat gcgcccaaca tccaacaagt
       61 tcagcttctg ccagttgacg ataagcttct agcggttcgc agtttaccaa gcttagcgtc
      121 gccttgctcc taaaccttgg actgctatcg cataggcaga actagaggac taaagtccta
      181 agttactgcc gcaacatatc acaatgaaca tagccagaat aatctcctag gaattcttgt
      241 actaccgaac cactccgact ctgatcatga tggtacagcg tgattgcttg attctcaact
      301 ttcccagaca aaaaggtcca atagtaggtc agatggctat gactctctag aacccgataa
      361 gaagtttcat ccgcatgaag taagggctga gtcaatagtc tctctcgcaa gaggttataa
      421 aggggcttta ggaataggag ctttcacaag cttatccaga tgattatctt ttactcgtta
      481 tggacaatgc tatatggcat aaatcaagta ccttaaagat tccgactaat attgg
//
LOCUS       DAVBNO010000127          522 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVBNO010000127 DAVBNO010000000
VERSION     DAVBNO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.127"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            1..522
                     /locus_tag="VQ846_002081"
                     /pseudo
     CDS             1..522
                     /locus_tag="VQ846_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          201 a           88 c          108 g          125 t
ORIGIN      
        1 aaatcgttac tattctcctg atttaaaaca agaaatgatt cataaagtct gacatgaagg
       61 ctagactaaa gatagagttt ctcttgaata cggtctccca agtcgtacga tacttcttaa
      121 ctggctagca caatacagga aaaacgggta tactattgtt gagaaaccaa gagggagagt
      181 acctaaaatg ggacgtaagc caagaacgag acctgaagag aggacagaat tagaacgtct
      241 tcaagcagaa aatgagtacc tgagagcgga gaatgccatc ctaaaaaagt taagagaact
      301 ccgattgaag gaggaaaaag agaaagaaga aagatagaaa ttgttcaaga attaatgact
      361 gagttttcgt tagatcttct tctaaaagcc attaaactag ctcgttggac ctactactat
      421 cacttgaaac agttagacaa accagataag gaccaagagc ttaaagctga aattcaattc
      481 atttttatcg aacacaaggg aaattatact tatcgccggg tt
//
LOCUS       DAVBNO010000128          513 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVBNO010000128 DAVBNO010000000
VERSION     DAVBNO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.128"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            1..513
                     /locus_tag="VQ846_002082"
                     /pseudo
     CDS             1..513
                     /locus_tag="VQ846_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT          154 a           99 c          114 g          146 t
ORIGIN      
        1 tatgaggagt tcggactcga ctctctcctt caagaaacac gtggtggtcg taaccatgca
       61 tatatgacag ttgaggaaga gaaagccttt cttgcccgcc atttgaaggc tacagaggca
      121 ggagaatttg ttacaattga tgccttattt caggcttata aaaaggagtt aggtcattcc
      181 tacacacgtg atgccttcta tcaactgttg aagcgccatg gttggcgaaa tattacgcca
      241 cgtccagaac atcctaagaa agcagacgct caaaccattg ttgcgtctaa aaataaaatc
      301 tcaattcaag aagacaagaa agcgttttaa atatagtaga cgttttcata aggttcgctt
      361 gatgtaccaa gatgaggctg gtttcggtag aatcagtaaa ctgggatctt gttggtctcc
      421 aataggagta ggtccacatg tccatagtca ctatatacga gaatttcgct attattatgg
      481 agctgttgat gcctatacag gcgaatcatt ttt
//
LOCUS       DAVBNO010000129          488 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVBNO010000129 DAVBNO010000000
VERSION     DAVBNO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.129"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            379..488
                     /locus_tag="VQ846_002083"
                     /pseudo
     CDS             379..488
                     /locus_tag="VQ846_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          195 a           74 c           77 g          142 t
ORIGIN      
        1 gactaaaatc aaaaagcatt atataatagt gatatgaaat caactaaaga agaaatccaa
       61 accatcaaaa cacttttaaa agactctcgt acagctaaat atcataaacg ccttcaaatc
      121 gttctatagt aaaatgaaat aagaacagta caaatcgatc aggacagtca aattgatttc
      181 taacaatgtt ttagaagtag aggtgtacta ttctagtttc aatctattat atttcgtctg
      241 atgggcaaat cttataaaga gattatagaa cttttatagt agtttgaaat aagatgtgaa
      301 caactctatc aggaaagtca aattaattta tagaaatatt ttagcagcca aggtgtactg
      361 ttatagattc aatacactat agactgtaat caaacaacga tttggcgaaa tgtaaaaaaa
      421 tatgaggagt tcggactcga ctctctcctt caagaaacac gtggtggtcg taaccatgca
      481 tatatgac
//
LOCUS       DAVBNO010000130          476 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVBNO010000130 DAVBNO010000000
VERSION     DAVBNO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.130"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(91..228)
                     /locus_tag="VQ846_002084"
     CDS             complement(91..228)
                     /locus_tag="VQ846_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET3076578.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEALNIRLEVK
                     N"
     gene            complement(372..446)
                     /locus_tag="VQ846_002085"
                     /pseudo
     CDS             complement(372..446)
                     /locus_tag="VQ846_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410533.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope"
BASE COUNT          170 a           79 c           69 g          158 t
ORIGIN      
        1 tatgtgcttc aagtatataa aaaggcgtaa aaatacgcct atagataatg gggttgaaat
       61 aggtttattg ttgatgagat tgtagataat tcaatttttt acttccaatc gaatattcaa
      121 agcctccacc ttttctgcct gtaattgttc atcataaaat tcaatatctt caggattttc
      181 cccttgacaa cctcggcaga aatattcttc cgctcgatca ggattcaaaa atcgacaagc
      241 acaaacaaaa cagtcgccat catcatttat tgagataata tagtagattg aaataagatg
      301 taaacaaatc gattaggaaa gttaaattaa tttctagaaa tttttagcag atgtagtgta
      361 ctattctagt ctcaatttac tatggcttca aatatatctt tcgaaaaaat atttacagat
      421 gtgtaatttt gaagcttgca aaagttagcc ccaattattc acccctaatc taaaaa
//
LOCUS       DAVBNO010000131          445 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVBNO010000131 DAVBNO010000000
VERSION     DAVBNO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.131"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            1..445
                     /locus_tag="VQ846_002086"
                     /pseudo
     CDS             1..445
                     /locus_tag="VQ846_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          172 a           72 c           87 g          114 t
ORIGIN      
        1 aaatcgttac tattctcctg atttaaaaca agaaatgatt aataaagtct gacatgaagg
       61 ctggactaaa gatagagttt ctcttgaata ctgtctccca agtcgtacga tacttcttaa
      121 ctggctagca caatacagga aaaacgggta tactattgtt gagaaaacaa gagggagagt
      181 acctgagagc ggaaaatgcc atcctaaaaa agttaagaga actccgattg aaggaggaaa
      241 aagagaaaga agaaagacag aaattgttca agaattaatg actgagtttt cgttagatat
      301 tcttctaaaa gccattaaac tagctcgttc gacctactac tatcacttga aacagctaga
      361 taaaacagat aaggaccaag agcttaaagc tgaaattcaa tccattttta tcgaacacaa
      421 gggaaattat ggttatcgtc ggatt
//
LOCUS       DAVBNO010000132          377 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVBNO010000132 DAVBNO010000000
VERSION     DAVBNO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.132"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(1..377)
                     /locus_tag="VQ846_002087"
                     /pseudo
     CDS             complement(1..377)
                     /locus_tag="VQ846_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          100 a           67 c           71 g          139 t
ORIGIN      
        1 agtacttact ggaatggcaa attcagtcac atctgtgtag cactgttcca ttgttttaga
       61 gccttcaaat tggccttgaa tgagattctc tgccttcttg ccaacgtctc ctttaatgag
      121 aagaatattt tcgtttctgt cgcgttttag cttgtaaatt gagtactttc atcaagcctt
      181 gaactctttt atgatttacc agataagcac gatttcttag ttctaaatga atacagcgat
      241 aagcataatt tcccttgtgt tcgataaaaa tggattgaat ttcagcttta agctcttggt
      301 ccttatctgg tttgtctagc tgtttcaagt gatagtagta ggtccaacga gctagtttaa
      361 tagcttttag aagaaga
//
LOCUS       DAVBNO010000133          348 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVBNO010000133 DAVBNO010000000
VERSION     DAVBNO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.133"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(<1..>348)
                     /locus_tag="VQ846_002088"
     CDS             complement(<1..>348)
                     /locus_tag="VQ846_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3076579.1"
                     /translation="KTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTILPHSAYSETL
                     YQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIKE
                     MKEGFFGDKTDSST"
BASE COUNT           87 a           74 c           84 g          103 t
ORIGIN      
        1 ggttgaacta tccgttttat cgccaaaaaa tccctccttc atctccttga tgaaattctc
       61 ggcttgacca cgtccacgat aaagctgaaa ctggtcttgg cttgttccac tcgtcatatt
      121 tgtaacgaga gaaataacat cgtagaacaa gtttccttct tttcgttcag agaactggca
      181 gacacgacgc ttgtgcgacc aagatcctgc ttgatagaga gtttctgagt aggcggagtg
      241 gggcaagatg gttaagtcct catcctgtgg gcaagggagg gaaaggtctc caagacggct
      301 cagaacagta tttttcttga gttttatgag gtagtattgc cctgtttt
//
LOCUS       DAVBNO010000134          297 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVBNO010000134 DAVBNO010000000
VERSION     DAVBNO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.134"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..>297
                     /locus_tag="VQ846_002089"
     CDS             <1..>297
                     /locus_tag="VQ846_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET3076580.1"
                     /translation="GKLERYDIVLTTRGTVGNVAYYDELIKYKHLRINSGMVILRPKT
                     PNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQNEFADFVV"
BASE COUNT          109 a           56 c           47 g           85 t
ORIGIN      
        1 aggcaaactt gagcgttatg atatagtctt gacaacaaga ggtactgttg gaaatgtagc
       61 gtactacgat gaattaataa aatataaaca tttacgtata aattcaggta tggtaatatt
      121 acgtcccaag acaccaaatc taaatcagaa atttattatc catgttttaa ggaataataa
      181 ttatagtcga gtgatatcag gaagtgctca gcctcagtta ccaattacaa aattaaaaaa
      241 aatacttctc cccctccccc cactagccct ccaaaatgag ttcgcagact ttgtagt
//
LOCUS       DAVBNO010000135          297 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVBNO010000135 DAVBNO010000000
VERSION     DAVBNO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.135"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            116..>297
                     /locus_tag="VQ846_002090"
     CDS             116..>297
                     /locus_tag="VQ846_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HET3076581.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCR"
BASE COUNT           95 a           57 c           49 g           96 t
ORIGIN      
        1 cttagatcct ggatggtttc ttttttcacc caatgggtgt tttttactag acaaaaaaga
       61 gtttcccctt tatggtataa gtgtagaaaa aaacacaaaa agaaaggaaa ctcacatgaa
      121 cagtttacca aatcatcact tccaaaacaa gtctttttac caactatctt tcgatggagg
      181 tcatttaacc cagtatggtg gtcttatctt ttttcaggaa cttttttccc agttgaaact
      241 aaaagagcgg atttctaagt atttagtaac gaatgaccaa cgccgctact gtcgtta
//
LOCUS       DAVBNO010000136          284 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVBNO010000136 DAVBNO010000000
VERSION     DAVBNO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.136"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
BASE COUNT           86 a           55 c           79 g           64 t
ORIGIN      
        1 tctgaggtgt aagtcctcgg tgggcacctg ttaccggtga acccaatagc gattcccaag
       61 cctgactatc gtgaggtagc ggattaaaac ggtctgggga tagaccgttt taaatctgac
      121 gctggaaata agaatcgtca gaggaaggga tagcgaactc gtggctctac gaacaggaac
      181 gtgatagtaa ggcgtatata gcggataagg gggcctcaaa ctctaaagtt caaaaaggta
      241 gtcgtaacct atatgcgtaa atcacgagag caactgaatt tgtc
//
LOCUS       DAVBNO010000137          267 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVBNO010000137 DAVBNO010000000
VERSION     DAVBNO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.137"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            complement(<1..>267)
                     /locus_tag="VQ846_002091"
     CDS             complement(<1..>267)
                     /locus_tag="VQ846_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET3076582.1"
                     /translation="ELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEGVAYNAHYRAHGY
                     HPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLL"
BASE COUNT           91 a           49 c           60 g           67 t
ORIGIN      
        1 aagagaagtt gattaaaccg ttctaaaaca ggtgtgataa agctgtctgc ctcttcagaa
       61 caataacgat taccaggacg aagctgggca ttgaaacaat aacctgtctt cccctcgaaa
      121 gcataaagag gatgatagcc atgagcacga tagtgggcgt tataagcaac accttcttgc
      181 ttgccataag ttgtgaaatg ggtagaatcg atatctacaa tgagttggtt tagctggtga
      241 aactgtaaaa agaattcgac caattca
//
LOCUS       DAVBNO010000138          251 bp    DNA     linear   BCT 11-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain NIG 460-09
            SAMEA1012576-rid20172633.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVBNO010000138 DAVBNO010000000
VERSION     DAVBNO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1012576
            Sequence Read Archive: ERR059373
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001979963.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001979963.1
            Genome Coverage       :: 629x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 00:07:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,092
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 1,911
            CDSs (with protein)               :: 1,911
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 2 (5S, 23S)
            tRNAs                             :: 40
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 78 of 134
            Pseudo Genes (incomplete)         :: 54 of 134
            Pseudo Genes (internal stop)      :: 34 of 134
            Pseudo Genes (multiple problems)  :: 27 of 134
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1012576-rid20172633.denovo.138"
                     /strain="NIG 460-09"
                     /db_xref="taxon:1313"
     gene            <1..>251
                     /locus_tag="VQ846_002092"
     CDS             <1..>251
                     /locus_tag="VQ846_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET3076583.1"
                     /translation="PIRHPERLGKPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDI
                     TSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKG"
BASE COUNT           84 a           51 c           60 g           56 t
ORIGIN      
        1 ccgattcgtc atccagaacg tttaggaaaa ccaaatgcgc aaattaccta cactgatgat
       61 gagattcaag tagccaagtt ggcaggcaag tacacaacag aagacggtta tatctttgat
      121 cctcgtgata taaccagtga tgagggggat gcctatgtaa ctccacatat gacccatagc
      181 cactggatta aaaaagatag tttgtctgaa gctgagagag cggcagccca ggcttatgct
      241 aaagagaaag g
//
